LOCUS       DANLYI010000001        43449 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.001, whole genome shotgun sequence.
ACCESSION   DANLYI010000001 DANLYI010000000
VERSION     DANLYI010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 43449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43449
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.001"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            complement(65..940)
                     /locus_tag="PXD81_000001"
     CDS             complement(65..940)
                     /locus_tag="PXD81_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDL5536078.1"
                     /translation="MWIWFTLLAAVAQAGRNAFQKQLSVNVPVLGVTLARFFYAVPLA
                     SGYLYFLYQYDDNLNIPHFPPLFFVYAIGAGLAQILATSLMVQLFHLKNYAIGVGLAK
                     SEAVWAALLGVLFFGVTISGIGWLGVVLGGVAIFLMTGSGNLRQLSWKTLFLGIASGL
                     CFALTSLWVREASLQLNSHYLLSAAWVLFSVISFEAVILVIYLALLQKETLIKFFRFP
                     KLGMLTSLFSFLGSFGWFNAMSLNHVAFVKTLGQVEIFFILGISYFVFKERLKIQDFI
                     GLILVVIAAILVVLG"
     gene            1048..1884
                     /locus_tag="PXD81_000002"
     CDS             1048..1884
                     /locus_tag="PXD81_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HDL5536079.1"
                     /translation="MLPHIYIGTGGYADTDLIGTLYPFGTAKEDFLFVYSQHYDTLEI
                     NSTFHAPIGQKAFEGMVQKAEGRLNFSVKLHQDFSHQRTATLDQAQLFLNALAPFTAE
                     GLLANLFVQFPTQFERMPLNRRYLAELCQWFADYPLAIEFRHPSWHIPAVFDTFAKSS
                     NLIWCNVDYPQNIGLPAFHFFANQRTAYLRLHGRNPNWWKGQSAAERHDYRYSDEELK
                     GLAELLFSRRTEFDRLYLYFENTTKSHAFYNIQTLKGFLAEKGFQIKPVPENLLGQQS
                     LF"
     gene            complement(1959..4811)
                     /locus_tag="PXD81_000003"
     CDS             complement(1959..4811)
                     /locus_tag="PXD81_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HDL5536080.1"
                     /translation="MVQAVSFSHFFAIHYFYFFLHKTKGQCTAKPLFSILLFHLFPKN
                     SAKFLLFFYFLVLMVLYSNEVANGKLLITGGTNLNGTLSAEGTSEIILSGRPTPHAYD
                     KVANKEVLIEGDWINRSFNATIFAAKDNGKLEISRNVANINGNFNLTDNATAQIGFTQ
                     GTSQACIRSDRTGVATCNINATLSETDLNSWERTKVAGNVSLADNSTFSLGSKADLTG
                     SITAQESTKVQLNDGSIANLTGLSTTGIFNGANGSTVNLSGVWTTLTNTVVDVLNVAN
                     GSQIHLNTPTATKQNTLTANTLTGNGIFKFVADLTKTASDIVTIKDTASGQFSIEIST
                     NGSAHSAQKIKLFETTNEEDFSLNLANEVRSGEYRYHLLKEGNVYYLNPEKEEEPKIT
                     QKGPSETADPLPEYTGDIEHSEKGPGETADPLPEYTGDIEHSEKGSGETAEPLPKYNG
                     NAHLIASELTAQTNAVLGVTTVLDHVIHSEGTQKQNTLTANTLTGNGIFKFVADLTKT
                     ASDIVTIKDTASGQFSIEISTNGSAHSAQKIKLFETTNEEDFSLNLANEVHSGEYRYH
                     LLKEGNVYYLNPEKEEEPKITQKGPSETADPLPEYTGDIEYSEKGPGETADPLPEYTG
                     DIEHSEKGPGETADPLPEYTGDIEHSEKGSGETAEPLPKYNGNAHLIASELTAQTNAV
                     LGVTTALDHVIHSEGTQNWKVWSKTDYQTTEHRSAGYGFNQDANVIHLGLEKALDNQV
                     TLGMILSQSKARNNFDHYYSGKGRLTMLSMYAKKTWQNGVFVAIDTGFGKASNRLTYQ
                     ANTVKLDRSVFVTGLSIGKAWESANVNIIPSFSARYHHLSSAGNQLVDAKIETNAVDL
                     LALQAGLSINKTLELNGLQIKPEIGSYFVDASHGKLRTRFNNLQIEQQMGRYFKQEAG
                     ISANYRNINAGIQAGFLTGNTLNKQRYISFKVTYEW"
     gene            complement(4859..5536)
                     /locus_tag="PXD81_000004"
     CDS             complement(4859..5536)
                     /locus_tag="PXD81_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL5536081.1"
                     /translation="MNSIIRKEWFTASEVAGMGNLPEQPSNVTRRATKNNWLKRQIEG
                     KRGVAFEYHYSSLPISVQQELGFAQTAVKEEPRTLVAAQTADEMERVPFYNVQASAGF
                     GAFNSEVYAPDDYIGLSKRWLDVRGFYLSYLAFITASGDSMYPTISDGDMLLVNRATT
                     QPKDGKIYVFRQGEQLWVKRVQGIMGGIRLISDNKELYSPIDVIFDETLDFEVIGQVV
                     YIGHEMI"
     gene            5750..5968
                     /locus_tag="PXD81_000005"
     CDS             5750..5968
                     /locus_tag="PXD81_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL5536082.1"
                     /translation="MARNKRVRDMSNFEIRGELMKIGKSLSQLGIENGLAKTTVRNAL
                     DKPYPKGEQIIAKAIGKTPQEIWPSRYE"
     gene            5978..7948
                     /locus_tag="PXD81_000006"
     CDS             5978..7948
                     /locus_tag="PXD81_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mu transposase C-terminal domain-containing
                     protein"
                     /protein_id="HDL5536083.1"
                     /translation="MKEWFLAKEIAGLSGVPELPNSVSRLATKEGWQKRQIQGVRGVT
                     YEYHLTSLPIETQQQLRLNAALAVIPQAAELQPKRDDPALIARLNNATDKGRDKAKGK
                     AEACMQLQAFLDQGFSYTQAEAGAATAKNVSQGSLKNWYYKVKGHPVHLWQAILISES
                     GRSKKPQLKAKITEEAWDCFLADYLRPEKPDLRASYRRTQAIAKQYGWQMASLQTFQR
                     RVLAEVPYEVILLKREGANAVAKLVPALQRTVKDILAGEWINGDGYQHNVFVKWHTGE
                     IVRPKTWFWQDVRTRKILGYRTSISENTDSIRHALMDVIFNVGIPKTLTLDNTRAAAN
                     KAMTGGIENRYRFKHTELDPKGIMPILGIDVHFTSILYGEGHGQAKPIERAFGRGGIG
                     EKIDKRPELSGFYTGRNAQETPDNYNGGKDGVDYNTFLKAIAAGIEEYNSQTERHTEM
                     CRGELSFNQVWERDYHPSNVRQASPEQLRLLFLQAETVSIKRNGSFTLKAAGKLYGLT
                     NVYWAESLIGITDKKVVARFDPDDLHGNVYVYNLEGQFLAEAVCREAKGFGDTSASRE
                     QGRLYQKVVKSAKAQAEALELLEAHELASLAPQVDVPEPIEKKVKEVLVKEDFIVDFN
                     TVRKATVVEETEEISIFDEVLLKQEKQLRIVE"
     gene            complement(8128..8616)
                     /locus_tag="PXD81_000007"
     CDS             complement(8128..8616)
                     /locus_tag="PXD81_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5536084.1"
                     /translation="MLFRSLTDDEIHQIALDSARATFSGFKLKRANAIVMKNCITLFK
                     LSTINFCLAEYHSSFHHLTSIDDLDGIMPTVKKVAQMLSLQESKALIWLLYLKWLFVM
                     RQRSLQVLLENSADLIQGEVIAQPLPTFVKAVLAFQLETYHGLPEWIPEFDSAEFWQS
                     LS"
     gene            8648..9589
                     /locus_tag="PXD81_000008"
     CDS             8648..9589
                     /locus_tag="PXD81_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDL5536085.1"
                     /translation="MTLIDQIKQHIAESGSSQGKVAKEAGINAGALSAYLNENYKGNN
                     EELEAKLIAYLERIEAKKREFVEAPSFIETKTANQIFGSLRFAQNTGVLAIIHGASGV
                     GKTQAAREYRKRYANVWLVTASPSRSSLSEVLYEIALELGMNDAPRRKGTLARLIVRK
                     MKGTAGLLIIDEADHLPYEALEELRLLQEEVVNESDIGAGVGLVLIGNDKVYTRMKGG
                     INPAHEYGRLWSRSAKRTSIQKTKKEDTQAVAKAWGLSENQEALKVMQAITETGGGLR
                     ILTHTLRLAAMVAKDRMIPLTADLIQLARKDLLSTGD"
     gene            9592..9906
                     /locus_tag="PXD81_000009"
     CDS             9592..9906
                     /locus_tag="PXD81_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5536086.1"
                     /translation="MRTPKPKHSFNRTNQAACRFLKQTQKAIIRLNSLGFTVLNIDFT
                     RIKPRIEVEIGNNKHIAQGLIYEGKAYRYSFGKSEDLGRWEGYYTMLEGIRVWWRQAA
                     IH"
     gene            9918..10154
                     /locus_tag="PXD81_000010"
     CDS             9918..10154
                     /locus_tag="PXD81_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5536087.1"
                     /translation="MKTLALLTALLGLVGCDLVQSKQAKNYIGNNLTEICIDNVVYLI
                     YSGDKKGGITPKVNKDFYPYTCTNKEERQNGKTR"
     gene            10138..10431
                     /locus_tag="PXD81_000011"
     CDS             10138..10431
                     /locus_tag="PXD81_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5536088.1"
                     /translation="MAKPDETPITKAECQSQLAELGVQYKKLPMAITRHICNATTNIH
                     GKVIKVSVVERVGYGVQITAQGNEKSCLVTYEAMLGMAEAMGLFDEVKEQNND"
     gene            10553..10771
                     /locus_tag="PXD81_000012"
     CDS             10553..10771
                     /locus_tag="PXD81_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005606434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HDL5536089.1"
                     /translation="MNDELAAISASYAQHNRYQHYAEKAAEAERKGNYKEAQDHWEVA
                     KLSAKKTANRDWAEQRAEFCKRMHNKPF"
     gene            10781..10963
                     /locus_tag="PXD81_000013"
     CDS             10781..10963
                     /locus_tag="PXD81_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005822929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5536090.1"
                     /translation="MTETVKAQLNSQLNEAIIQLIQAQKYLNQDDAIRSGVYIGTVQD
                     LLPKVHLKLLTANRKH"
     gene            10980..11369
                     /locus_tag="PXD81_000014"
     CDS             10980..11369
                     /locus_tag="PXD81_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5536091.1"
                     /translation="MAITEEQWKEIKQKLDSIIGRVKFRYKEHLLTVDVVQIKRSLKL
                     AVYVDGEIDGAWTKEGHEIRPYLEEVWYRKERPFFNAKEKKEYRGLMSKKKLNEKIVT
                     YSPMFPSPTALTRQYKKLDGLELIEVI"
     gene            11418..11705
                     /locus_tag="PXD81_000015"
     CDS             11418..11705
                     /locus_tag="PXD81_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5536092.1"
                     /translation="MNKNEFFKKYHLEGSLAILDLDANDDQSQFCYQALLEYRELPLD
                     TFLAEYSDDEDMTESEFIELMLDELEWIIRRAKNAKRFVKKRQKTMREEIM"
     gene            11824..12369
                     /locus_tag="PXD81_000016"
     CDS             11824..12369
                     /locus_tag="PXD81_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1018 domain-containing protein"
                     /protein_id="HDL5536093.1"
                     /translation="MTDKAKLIQLIHIAKSQLAMDDLSYREMLKRLTNKTSSTKCTVV
                     ELHKVLHELQTKGAKVKYFAKRSKKPTAYSPATGETAVKSQIAHKIRAVWITMGKHGF
                     LNDPSEKALNAYMRKVINNKTRPTILLNVSALEAGDASYMLEILKKWHKRVMIKALSK
                     SCTVDPKISYDELVEWYNEVM"
     gene            12356..12889
                     /locus_tag="PXD81_000017"
     CDS             12356..12889
                     /locus_tag="PXD81_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005693508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5536094.1"
                     /translation="MKLCRCPICHSDIHLEALVEDEAGRELLGKISQLTHGVAQPMVA
                     YLGLFKPQKSNLNNSRALKILNDVLALYPCSLLLAQALSETVVSIRKKRQQAMENGQK
                     IEPLPNHNYLKSVYETQKVHFAVVRTGKTERETMKAELEKTRNAIAYVQRFVDLGREE
                     VVKNSPEYQIWLAHKPK"
     gene            13054..13413
                     /locus_tag="PXD81_000018"
     CDS             13054..13413
                     /locus_tag="PXD81_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDL5536095.1"
                     /translation="MELVDIFEEKAPEVLTDLAKHIEVALEEKCQFSPEKAKQIGIDI
                     AQQMAQNWGGEVIYIPRNLLMALNERDMKIYEEFNGNNHRELARKYNVSMQWVYKIVK
                     KVHKDEVARRQMSMFEM"
     gene            13549..14058
                     /locus_tag="PXD81_000019"
     CDS             13549..14058
                     /locus_tag="PXD81_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 108 protein"
                     /protein_id="HDL5536096.1"
                     /translation="MSQNLTFRQIFDRLIGHEGGYVNHPQDPGGETHWGITKRTAVAN
                     GYMYAMKNMTREQAYQIYEKAFWQRYRCAELKPAVAYQFFDAVVNHGFGNASRMLQRA
                     VGVADDGIIGNITLQAINAKSESDLLMLFNAERLKFYTKLRNFPTFGKGWVNRVAGNL
                     EYAAQDNEV"
     gene            14061..14330
                     /locus_tag="PXD81_000020"
     CDS             14061..14330
                     /locus_tag="PXD81_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2644 domain-containing protein"
                     /protein_id="HDL5536097.1"
                     /translation="MAKLFKELLSTNGTLSTTSTVQFFGTLCVFGLMVYAVITQQPYA
                     ESLLNNVLIYLFGATTAKGVVTSYQAKIKGGIHKQQSTFKEEEGC"
     gene            14324..14671
                     /locus_tag="PXD81_000021"
     CDS             14324..14671
                     /locus_tag="PXD81_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2681 domain-containing protein"
                     /protein_id="HDL5536098.1"
                     /translation="MLMQQILGVIGVIAAILSYALFKSWQLKRERKKNAILQAEKQQQ
                     AVEIEQKKAEVKYVYISKKNNEKVTRSSASDIDDKLQQHGYFRDNNRLHGVQSDLSEP
                     CGYAGNETSDSCS"
     gene            14698..14850
                     /locus_tag="PXD81_000022"
     CDS             14698..14850
                     /locus_tag="PXD81_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5536099.1"
                     /translation="MKYIKKLRKRWQIWRFLKNHPEVAQRRDLLAIAIKQGVENPVGR
                     ARRREP"
     gene            14850..15185
                     /locus_tag="PXD81_000023"
     CDS             14850..15185
                     /locus_tag="PXD81_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2730 domain-containing protein"
                     /protein_id="HDL5536100.1"
                     /translation="MDLFTVFWESIRANFGFISALLTIVVTAFWLKLDSKYAKKNDLG
                     ELLEIAKSHDNRLTTLETKVENLPSAVDVEKLKTLVTDVKGDTKATSKQVDSISHQLG
                     LLLEAKLKE"
     gene            15187..15483
                     /locus_tag="PXD81_000024"
     CDS             15187..15483
                     /locus_tag="PXD81_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5536101.1"
                     /translation="MSLRDLLTQDQRLVILRSLAEAGYDANESIISDCLDLYGHDISR
                     DLVRTHLSWLEEQGLLTLERLRDGYMVASITQRGLDVAQGRVVVSGVKRPRPKI"
     gene            15506..16078
                     /locus_tag="PXD81_000025"
     CDS             15506..16078
                     /locus_tag="PXD81_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3486 family protein"
                     /protein_id="HDL5536102.1"
                     /translation="MSEKNTRGRASKVDLLPPNIKTQLAMMLRDKQYSQSQILEEIND
                     LIRDCGLPDEMLLSKTGLNRYASRMERLGAKIRNAREVAEIWTKQFGEMPQTDIGKTL
                     MEMVKQIAFETSLKLGEQEGGIEPKQLALLSSAIQRLEQAESLSFKREQAIRKEVAQQ
                     AAETAEKVVVQAGLSAETVRTIKEQILGIA"
     gene            16078..17634
                     /locus_tag="PXD81_000026"
     CDS             16078..17634
                     /locus_tag="PXD81_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase family protein"
                     /protein_id="HDL5536103.1"
                     /translation="MALLNNRPLNELAPECRSFLESIHAFNPNELLLGYQKRWIADDS
                     QLKIAEKTRRCGLTWAEAADNALIASTRKSDGGSDVFYIGSNKEMAREYIDAVAMWAK
                     AFNRVAGEIQEEIFEDEDKDILTYVIYFSSGFKVKALSSNPKNLRGMQGVVVIDEAAF
                     HEYLAEVLKAALALTMWGAKVRIISTHNGSENLFNELITDSRAGRKRYSVHTITIEDA
                     CRDGLYQRICQVVTKKTWSPEAEKEWIENLLNDTASEEDALEEYFCVPKFGSGLWLSR
                     ALIERQMSEATPVIRFTAKDGFSLVAEPTRYAEIAEWCELELEPILATLNPDLLHFMG
                     EDFARSGDMTSLVVLAQQQNLVRKVAFIVELGNMPYKQQEQIVMFMLKRLPHFAGGAF
                     DARGNGGYLAEAARDEFSTLIDCVQLSEKWYREHTAPFKAALEDGELEAIPKDADILA
                     DLRSFQVVKGVPRIPDKRTKSADGKSKRHGDTAISLLLAHYASRQLVQMPVEPVSRRP
                     RHSQQLTEGY"
     gene            17753..19207
                     /locus_tag="PXD81_000027"
     CDS             17753..19207
                     /locus_tag="PXD81_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF935 family protein"
                     /protein_id="HDL5536104.1"
                     /translation="MAKKNNLISEIATRARSIDYWAFGYYLPNPDPILKRMGKDISVY
                     RELLSDGQVRSGVRRRKAAIKGLEWRITTTNNEKVDEQIYQIFNRLPMNNLITEMLNA
                     ALYGYHISEVIWAKEGELFVPTEIIGKKPEWFMFDDENLLRFRTKENWADGELLPDDK
                     FLLTTQEATQDNPYGLGDLSLCFWAATFKKGGFKYWLEFTEKYGSPWLIGKHPRQAHN
                     TDKERLADALEGMISSAIAVIPEDSSVEIKDAAGKSASTDAFEKFLNFCKAEINIALL
                     GQNQTTEQESNRASAQVGLEVMEDIRSDDQAMIEATFNTLLQWIVKYNFNVEQLPKFE
                     FYEQEAINTDQVERDSKLHAMGVRFSKAYFEREYGFEAGDIEVQAVEKAEKSANTTEF
                     SEGQRRTQVPMPVPILEQLEHEGEAMVDDWLQGVRDKLAQADSLEDFQSQLDSLIPEL
                     SFAEYGELLALGSTVAELAGRQSVNDEIKAKRNA"
     gene            19200..20477
                     /locus_tag="PXD81_000028"
     CDS             19200..20477
                     /locus_tag="PXD81_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head morphogenesis protein"
                     /protein_id="HDL5536105.1"
                     /translation="MRKFTFKQQAQYFEKKLNLRTNSYLDILGEEHDYFFVVAGANRN
                     EVLSEFRKAVDEAIHNGETLENFRKRFDEVVAKTGWEYNGGRNWRTRIIYDTNVYGAY
                     NRGRLQQHLELAEDMPYWEYQHNDNAHPRPQHVAWDGLVLRYDDPFWKYHYPIKAYGC
                     HCTVQALDDFDLKHDGKTVSPPPEIEFEQKTVGVRTGNSRVVTVPKGVDVGFTPHNFD
                     NLKAGRTANVDKLLMQKMVTADPIFASKAVSDLLAKEPVAKLHNQAFKNWVNDVANAK
                     GSALSQVENMQLLGVLPVAVIERLQQINLAPQSAIIAITKGDMVHALRELKQNTGIAV
                     PLEFWEKLPAKLQYPNAILLEKQQAETTLLFIYDVAQTKIAVKVNYDATLQDRHGAKG
                     RQKVLLNRAVTGSLFQDTTGLKSSKYEVLLGEM"
     gene            complement(20679..21023)
                     /locus_tag="PXD81_000029"
     CDS             complement(20679..21023)
                     /locus_tag="PXD81_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5536106.1"
                     /translation="MMVNAEQAIELVYDLLLSRQWLVTKAEKLPLDPLSEKEAVMFLY
                     TLDQQTEASWLQLTPEQRATANGLIMDFIAKCLTSTKQWLVSDNIVPELQAIEIIKHE
                     IFLSHNSLVMPN"
     gene            21087..21551
                     /locus_tag="PXD81_000030"
     CDS             21087..21551
                     /locus_tag="PXD81_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HDL5536107.1"
                     /translation="MIKITLNNTQAVKSLQQIANQLEQPRRLYGILGETLKKIHTARF
                     KAEQDPDGNNWKPLASSTLALKRKRGKSTKILRQDGYLADKTAYNVTSDNVEFGSQEV
                     YARLHQFGGKAGRGKKVTVPARPWLGVGDKDEQLLLRKAEWHLGQIIGRMGK"
     gene            21786..22901
                     /locus_tag="PXD81_000031"
     CDS             21786..22901
                     /locus_tag="PXD81_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HDL5536108.1"
                     /translation="MTQIEIFKAGKRYDANGTLIDITPEMLQQTVAAYNPEFHEAPLV
                     IGHPKSNNPAWGWVKALSLEGDVLKAEVDQLDAEFSEMVASGKFKKVSAAFYLPDSPN
                     NPHKGVLSLRHVGFLGAMPPAVKGLKQVEFAEDDDFVVFSEWGVASLFSRFRDWLVSE
                     FGLEKADKALPPHEIDWLKEEAMRSQISKQVHAEQATPEPIFNEPAQPEGEPEMSAEE
                     KEELERLKAENEQLKAEKAQAEAEKMEAELEAEKAGNAEFCEGLVKQGKLAPVAKAAF
                     VQALNNLSVLKAGREPEFNEGEDVVSQFKSAMSQSPQIIQFGEYATADKANPQEAEAV
                     EYAEGTDPVSIEADQKVRAYMKAHNVGYAAAFNAIYR"
     gene            22932..23858
                     /locus_tag="PXD81_000032"
     CDS             22932..23858
                     /locus_tag="PXD81_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007524268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HDL5536109.1"
                     /translation="MTTHNLQKLRVQDPVLTELAQGYHNNELVAETLMPTVEIEKEAG
                     KIPTFGRLAFRLPSTVRNLRGSSNRLDPEDIGAIDVALEEHDVEYAIDYREENEAIFS
                     LRQFALNTTQDVIALGREKAVATLALDESRYETDNKVTLSGTSQFSHKDADVFKVFDT
                     AIRAVKRAIGRKPNVCVISGDVWAALKEHPAVVEKLKYSQVAIVTPEVFAKLIGIETV
                     KIGEAVYEESGQLKDIWSKDIVVAYVAPRSTGGKGTVYEPSYGYTVRRQKGLFVDTYK
                     ESGGKIEVVRTTDIHKPHLVGAAAGYLIKAAV"
     gene            23927..24208
                     /locus_tag="PXD81_000033"
     CDS             23927..24208
                     /locus_tag="PXD81_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5536110.1"
                     /translation="MAKFIVLHTPILHDGKRYEVGAEIELTAEQAENNAINLKAVEAV
                     PKPPKNASKPEDEPKPEGEPKPEDAVTEDDVKLAEAKIAEAEQPKGNKK"
     gene            24208..24642
                     /locus_tag="PXD81_000034"
     CDS             24208..24642
                     /locus_tag="PXD81_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1320 domain-containing protein"
                     /protein_id="HDL5536111.1"
                     /translation="MYVQIDELLTAFSRKIVVQLSNDEPTATEPDLKMLETAVKVANE
                     RIDAALRSRYTLPLADVPTLINQHALTLARYWLYARRPETKMPDTVKETYQQAVKELE
                     QIATGKLHLGIAESSQILEDDVLADNSEYEVRANARIDTSRY"
     gene            24648..25145
                     /locus_tag="PXD81_000035"
     CDS             24648..25145
                     /locus_tag="PXD81_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gp37 family protein"
                     /protein_id="HDL5536112.1"
                     /translation="MSATQPILEDICQHLRQALPEWEVELFPNDPSTYHLSHINGAVL
                     ISYLASKFEKPRTTQAVLQVRHVQIALTVLTRDLHNDVGALDLLDKLRLLVVGFKPQN
                     CGECWLIDEFFNGSSEETGLWQYQLILQTETQQVQHQAVENLPKFVTAHLRRQDQPGS
                     PGLKT"
     gene            25230..26615
                     /locus_tag="PXD81_000036"
     CDS             25230..26615
                     /locus_tag="PXD81_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HDL5536113.1"
                     /translation="MTFHHGSETERVNGGSVPVSVVDSAIIGIVGTAPIGAVNELTVC
                     LTKKDFAKFGTILNQGFTLPDAFDILARYAAGKVYVVNVLDPSRHRTTVTDEVLTLDR
                     NTLTAQTAKTGIIAVTRVKDGANVLNADRYSVDLQTGVVTFVSAPSAPKISYIYADPS
                     KVTEEDIKGGVDSLTGKRKGFELLRDGFNLYGADAKILICPEFDKTASCAAALATLAD
                     QLHAKAYIQLPKGTSLSKAIQGRGPAGTINASASNENVRHFFPYALGSSNTLESLATH
                     AAGLRMLIDVEKGYWFSTSNKELKGVIGLEIPLTARVDDKQSETNLLNAVGITTIFNS
                     FGTGFRLWGNRSSNFPTVTHISNFEVASRTGDIIDESIRQAELQFIDLPIDDALLDSF
                     LETIDTFLRTQKSLVGYSVSLDYEYDLVDAFSRGQVPIVYEYTPKIPGERISNKSVMT
                     RKYLANLVSQR"
     gene            26626..27141
                     /locus_tag="PXD81_000037"
     CDS             26626..27141
                     /locus_tag="PXD81_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail tube protein"
                     /protein_id="HDL5536114.1"
                     /translation="MSIAINQIVNGNVYINGNSQMGRVNEVKIPDIEFEFIEHKGLGM
                     HGVIKLPAGTNAIEAEITWDSFYPEVRAVMLNPYKYTQLQIRSNLQVFNSQGLAAEEP
                     MVTIMNVSAGKIGGTGHKNKENAEFADTLNVHSIKQTVAGRELLFIDVLANIYRVNGV
                     DVLQKFRTNIG"
     gene            27237..27551
                     /locus_tag="PXD81_000038"
     CDS             27237..27551
                     /locus_tag="PXD81_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HDL5536115.1"
                     /translation="MANAAQQVLTNLRVQTTYKLQYPIALPDGRVIETLDVRRPKGKD
                     FRLLDEKGFDPEKDGVKILRFYIQQLTTLVPEDIDELDAADIKGLNNLLEDMLIEGKS
                     DK"
     gene            27548..27700
                     /locus_tag="PXD81_000039"
     CDS             27548..27700
                     /locus_tag="PXD81_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005658873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GpE family phage tail protein"
                     /protein_id="HDL5536116.1"
                     /translation="MSELDTVYADLAWWFKFSPSELLELDLVDIPKWVEQMNRQIKAG
                     YGQILR"
     gene            complement(27679..27981)
                     /locus_tag="PXD81_000040"
     CDS             complement(27679..27981)
                     /locus_tag="PXD81_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5536117.1"
                     /translation="MLTKLNNRITEWAGFEACVTRTECTAKWLLAVALVGWSIFYWLD
                     AIQSIQSLSEGVSFVLIYGLILTILAWVGVYLAWGVAILLTLATEPFIWLYRKICP"
     gene            28029..30686
                     /locus_tag="PXD81_000041"
     CDS             28029..30686
                     /locus_tag="PXD81_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HDL5536118.1"
                     /translation="MANNLAIGMVIGASLSSGFHGAFSGAKKKLEQLEQAVAKNQQSH
                     SKLGSELTALRSKQATLYAEMSRASSKGGAGLALMQQEYKKIGKEIANISREQRKWST
                     ELEKSIAKQTKLRNAMERHTQAKENRDELKGKIIGTVASAAMGVGVMKTYMEQEEAAN
                     NLKISMLKADGSFGKFEEIGKIADTLGTDLPGTKKDFYKLAMALKKQGVSDDVLTGGA
                     LKTSAELNVLLDMDQESGGEFLAKFMESHRLNENELPQAADYLQRAMFAGGLSKEQMY
                     ESMKYYAPKLNSMKLTGAENTEKVLAIEAMAGQQGLEGSTFGTGLNMMLSRMNKGPEM
                     LKAAAKGMKAEAREMMEAVGVEFNFWDKNGSFKGIDGMLAEMTKFEKIRKKFGDKGVG
                     LVAEELFGIEGGRLADILAMKGQKGLDEMLSKMREQASLQDRINLKTNTLGAALESLG
                     GAWETFVGDIGSVFAEDIKGFSKGLQSAVEWLVPFVSKHKEAIKFVGGLVAGLVILRG
                     VLQAGSFVIQTVTMAMNALKIAAMTNPIVLVIGAIAAGAYLIYSHWDSIAPWFANLWQ
                     NVTAYFNQFWTWVKGVWSGVTDWVSSVWNGVATFFGQLWTTITSLFSVAWTNIQTIWG
                     GATAWFSGLWDGIKSAAFGMFDSISQKVSSVVASIKSFLGFGDDVDKMGAKIGNVATQ
                     VISKEHSGQLSKTADMAANTGFSSGGFTGQGGKYTPAGIVHKGEYVMTKEATSRLGVG
                     MLDSLNYGGAMGFTQPVLQPILGKQKGFFGSLWDDVKFGANFVGNLLGLNQGSLKTPD
                     FNPNATNASGQNSSIFSNYEPLNRNAVIQQESSQAPQIVVNFNPTINVNGNATQEAQS
                     QIMQQLQMSAYEFEKLLNRVLDQRSRRAY"
     gene            30696..31619
                     /locus_tag="PXD81_000042"
     CDS             30696..31619
                     /locus_tag="PXD81_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL5536119.1"
                     /translation="MYFMLGNIAFEPVNLTEFSEQHTAEFAEHAVLKGKPRLQAMGEK
                     LNELNFSLRLHHTIGGVESRYQALLEAKAKQAALALIWGSRYKGDYVIVDISSTTLFT
                     DGRGNALAREMQISLKEFVGDNDGGVLGEALNFGGGSLLGSILPSGAVTTLSQIKTAV
                     SRGVELYNSGKRLVDEVQNTVAIIRQLKNDPATALAYLPAALANLDGALGHFGNLTGS
                     SATLGGLQEKLPAAVVFSEEIGEIYQSLQTMRSSLGNASANSWDNWFTPAESALTEVT
                     DSFDHLAKPVAKMTAWIVLRTDEEAENDTVA"
     gene            31603..31830
                     /locus_tag="PXD81_000043"
     CDS             31603..31830
                     /locus_tag="PXD81_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail protein X"
                     /protein_id="HDL5536120.1"
                     /translation="MTQLLKHIVKQGERWDNLSYQYYGNAFEYGRIIDANPHIGFCEV
                     LPIGVTIYIPVLNVKPTNNENMPPWLRGRDA"
     gene            31823..32887
                     /locus_tag="PXD81_000044"
     CDS             31823..32887
                     /locus_tag="PXD81_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5536121.1"
                     /translation="MRNVPKPDFSLFYEKTNITAEIEPHLVQLTYIDHLEGQSDELTV
                     EFEDIQGKWIRQWFPTQGDKLKAAIGYQGEFLTDIGEFEIDEVEYSYKPSTINLKALS
                     TGISKANRTLKPKAYENTTLAQVVAKVADNLKLKVVGKIKAIPIKRITQYQERDVEFL
                     ARLAREYHHSFKIVGNQLVFTDKSELGQSEPVLMLEERDTISLRLRDRIKDTAKAVEI
                     SGYDTSGKKVVKKRKKAKALRPNIKQAKSASEDTLKIITRGESQEQIDARGEAALATQ
                     NEDQAAGDIELIGNPKLVAGATILLRNFGVFSGKYLIKSSRHTISRSGGYTTHIEVRM
                     LEFIADDLITLGMEAGNANA"
     gene            32874..33410
                     /locus_tag="PXD81_000045"
     CDS             32874..33410
                     /locus_tag="PXD81_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HDL5536122.1"
                     /translation="MQTHNFGATYQEGIVSAVDPKTHKVRCKIPALEDLETAWLSFLT
                     PNAGGNQFYCLPDVGELVAMLLDAQGEGGCVFGAIYNEQDPTPVADSDMWVKKFSNGT
                     EISHNRKTGDVVVKTNGHLTATAQGGATINANTVVNGTLHATGKITSDEEVSAPVVKQ
                     GTVELGTHKHGGSPEPNK"
     gene            33465..33827
                     /locus_tag="PXD81_000046"
     CDS             33465..33827
                     /locus_tag="PXD81_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HDL5536123.1"
                     /translation="MNRQTSIQLTHWQLAPSTDTVEVVQGIDDIHLCIVNILSTQKGT
                     DILRPEFGSDHFRYIDYPEDVAIPNFVREITTALQKWEKRIEVEEVLVDGSAPHFIFT
                     VMWHLTDDVYREIYRTTV"
     gene            33837..34940
                     /locus_tag="PXD81_000047"
     CDS             33837..34940
                     /locus_tag="PXD81_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HDL5536124.1"
                     /translation="MTEAIKIIDDDVKTVLAEAIADYEKRTGKILQPAHIERSIIQTY
                     AYREQLLRKGINHAFLQTFPQFATGIALDLCGETFGCYRLRDKPARTLLRFSVSGEHT
                     AIVIPKGTQVAINDELYFSTLNDDVITPLITYVEIDAECNQVGVIGNGWESGRVSKLK
                     TALATNYKVTVENIDITSGGLMQEEDEPYRKRILAAPEAFSNCGSIAAYDYHVRSVSQ
                     DIADVNISTPRGGLVRIAVLTQKGLPDTRLLDDIKKHVSAERLRPLCDMVEVIKPTER
                     RYQITARLILLDGYREDLVKAKAREALQLYLSDKTKKLGLDIVPSAIISALRVEGVYD
                     VNLIQPVKTIINETEWANCTAISVEVEEERRDG"
     gene            34933..35499
                     /locus_tag="PXD81_000048"
     CDS             34933..35499
                     /locus_tag="PXD81_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL5536125.1"
                     /translation="MASLHYADIIVRDPKYKALADLSKRLNLLDTSQIMTTLVDLLGD
                     ECIPLLAEKWSVTGYDGLLVADSENSKRALIKRAIELHQLKGTPWSIREVLRRLGFGE
                     VEIDEGLKNRLYESTYVSGIPENERWAHYAIRLSNPITNEQARNIRKVLRNFAPARCS
                     LAVLDYKADPIRYNNKATYNGTYNHGSS"
     gene            35509..37788
                     /locus_tag="PXD81_000049"
     CDS             35509..37788
                     /locus_tag="PXD81_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HDL5536126.1"
                     /translation="MAGVQETAQWENHVYRIEENDPVHGGENGVTNKPIKHLANRTLY
                     LRRLLTEAGQRINPKKITATSKNSNDLTGHTHEIDHASLTQKGIVQLNASIQSTSTTE
                     SATPSAVKAAYDKAATAEQKATLANEKVEAIKVGGRNLLIQSNTPHNGNAYSVRYALA
                     EAPKVGEEIVVTLWGELGSQRTGIGVYNSQGATELAKLVKIRDGVYQGKGIWRKPMSE
                     GRERTPNDTHLNVYFYPKTATDNNRIERIQLERGSIGTDWTPNEADLKAEISAIRTKL
                     IPVRGGNVATTLNAAKRSDWFREMQIPYQSGWGYYTQAFHNSGTQHNYSNLPLTGRYY
                     FIAELYHNSGYSHLRITYPSLKRTFESAINLNNEDLILDWREVVFLKDGLYQGTFKTT
                     GTLESAYRLRAARDDERYQPYLQMVDMGYDLANPPVGKNKIIGSVDYYVKNGTSESSK
                     ATMMAAAMPDKNVFWEVALWNAAQQRNVLFKGYSKTNNLSIGKTTDNERDRVQVNGTI
                     QATAPADNANNDQVPTTAWVRKMAKILADGKVSKAGDTMTGHLFIKNGEYSAVHTYNT
                     SGWFAKWEAAPLSDSHFAAIVYANDRETVINRVLIPKKNGTVALINDIADTVRAAVSR
                     SDKSRSRTFPLQTGKKYTTTGSVTIYPDGRIVQIFHLKNIKAAWFDYEASAVGDRHRV
                     VNIPLWTAMPNKIFDVDVKTVRPSNNASTYYIEAAEWLSAWQIYGNDTNKSSVNINLS
                     RFRGGKDEDMDLYVTVEGY"
     gene            37789..38391
                     /locus_tag="PXD81_000050"
     CDS             37789..38391
                     /locus_tag="PXD81_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5536127.1"
                     /translation="MAYFVNVIDEQGNYELIDDEFIGLYPDLDFNTLPKLTDKQYQTY
                     LAKANGKERFVNGEFVYEQIKVDMQAVISAEKSAKLAEINQKAQAFINDLAKYNETPK
                     FERDTWLEQAKEAKAWVADPTVQTPTLELIAQMRGIPLDTLRQKAYEKAMAYQTVAAI
                     VAGQRQGYEDRLEQAETLEQIQAIEPMYQLPQGANNEQTD"
     gene            38375..38650
                     /locus_tag="PXD81_000051"
     CDS             38375..38650
                     /locus_tag="PXD81_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5536128.1"
                     /translation="MSKLTKLKSWSYHVLIAIDQLFNALTGGAADETFSSRCYRGAVL
                     AENPKKRWRFWHSFLNCLFLDPKHCYAAYKAEVHRKQYPKAFRLGGI"
     gene            38650..38937
                     /locus_tag="PXD81_000052"
     CDS             38650..38937
                     /locus_tag="PXD81_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5536129.1"
                     /translation="MKKQNLKLVRGDDVSFRFSIKDSTGQPLALKDLWFDLHLVSYQG
                     EVLIALSTDDGSIETAEAGQMVLHFSHQQTARAEWTSANYDLQLTHDYTRV"
     gene            38947..39111
                     /locus_tag="PXD81_000053"
     CDS             38947..39111
                     /locus_tag="PXD81_000053"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5536130.1"
                     /translation="MTSIIELDLTDDLIIEVQLDSTIITGDSFLANLPDLLQIYNQAK
                     SDSSKELNND"
     gene            39104..39808
                     /locus_tag="PXD81_000054"
     CDS             39104..39808
                     /locus_tag="PXD81_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5536131.1"
                     /translation="MTKHKNHENHPIAITFYQLGKDVGDINRRLAVIEQREQSAQPSQ
                     PQAEAVTMPQQDAVAINNINAVLEAVEKYKTFEKYNTQWQERFEKFLSRNPNDYELSE
                     EQRQKLYHTMCQHLWQVNFTEINSKQYELPIPQQFQGKFGYIVKTSGGSTEISFELLS
                     ETHSTYLYPDSYIPEGGGDVMLKPAYIYLVIPDLVAMREPEVVGVSPLKLNILQKHLL
                     TPQKLQEQKPLNTKAN"
     gene            39977..40144
                     /locus_tag="PXD81_000055"
     CDS             39977..40144
                     /locus_tag="PXD81_000055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5536132.1"
                     /translation="MNKSLVALAVAAMASKAQTKQQASKQASKQASKQASKQASKQAS
                     KQASKQASIVR"
     gene            40141..40980
                     /locus_tag="PXD81_000056"
     CDS             40141..40980
                     /locus_tag="PXD81_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5536133.1"
                     /translation="MEKVFKQAPLPFSGQKRMFLSHFKQVLNANIEADGKDWTIIDVF
                     GGSGLLAHTAKREKPLARVIYNDFDNYAERLNHIKETNQLRQEIYQIVDEIIPKNKRI
                     SNEIKAKIINKINDFEGFKDLKCLSSWLLFSGEQVATLDELFKHDFWHCVRQSDYPEA
                     TGYLDDIEVVSESFHQLLPRFHDKEKVLLILDPPYLCTRQESYKQENYFDLIDFLRLI
                     DLTKPPYIFFSSTKSEFIRFIHYMVQNRKENWQAFEGAERIVVNASASYNGKYEDNMV
                     YKF"
     gene            complement(41017..43449)
                     /locus_tag="PXD81_000057"
                     /pseudo
     CDS             complement(41017..43449)
                     /locus_tag="PXD81_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249629.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="S6 family igA-specific metalloendopeptidase"
BASE COUNT        13479 a         8713 c         9906 g        11351 t
ORIGIN      
        1 ttattggttt tgcgtgggct tacgcccacc ctacaaagct agataaaaaa cgtaaaaatc
       61 cgatctagcc cagcaccacc aagattgcgg caatcactac taaaatcagc cctataaaat
      121 cttgaatttt aagccgttct ttaaacacaa aataggaaat accgagaata aagaaaattt
      181 ccacttgtcc aagcgttttt acaaaggcaa cgtggttcag gctcattgca ttgaaccaac
      241 cgaatgagcc gagaaatgaa aacaggctgg tcaacatacc taattttgga aaacgaaaga
      301 atttaatcag tgtctctttt tggagaagag ctaggtatat aaccaatatc actgcctcaa
      361 agctaatcac gctaaaaagt acccacgccg ctgaaagcaa atagtggctg ttgagttgca
      421 ggcttgcctc tcgcacccaa agtgatgtga gggcgaaaca taaaccgctg gctatgccga
      481 gaaagagtgt tttccacgaa agttggcgta gattaccgct gccggtcatt aagaaaatcg
      541 ccactccacc tagtaccacg ccaagccagc caatgccact gatggttaca ccaaaaaata
      601 acacgcccag taaagccgcc catactgcct cgcttttcgc cagcccgaca cctattgcat
      661 aattttttaa gtgaaaaagt tgcaccatta gcgaagttgc cagaatttga gccaacccag
      721 ccccgatagc atatacaaaa aacagcggtg gaaaatgggg aatgttgagg ttatcgtcat
      781 attggtataa aaaatagaga taaccgcttg ccaacggcac agcatagaaa aagcgggcga
      841 gcgttacccc caatacaggc acattgacgc ttaattgctt ttgaaaggca ttgcgaccgg
      901 cttgagccac ggctgccagc agagtaaacc aaatccacat aatgatgatg aaataagaaa
      961 agatagccaa atcttactct tttattctca gtttgcaagc ggttaaaaaa ccattaaaat
     1021 ttgcaaaaaa ttgaaagaat aaacacaatg ctccctcata tttatattgg caccggcggc
     1081 tatgccgaca cagatttaat cggcacgctt tacccattcg gcactgcaaa agaggatttt
     1141 ctctttgtct atagccaaca ttacgacacg ctggaaatta acagcacatt ccacgccccc
     1201 atcgggcaga aagcctttga aggaatggtg cagaaagcgg aaggtcggct gaatttttcg
     1261 gtgaaactgc atcaagattt cagccatcaa cgcacggcga ctttagatca agcacaatta
     1321 tttcttaatg cgttagctcc gttcaccgct gaagggttat tggcaaattt atttgttcag
     1381 ttcccaactc aatttgaacg tatgccactt aaccgccgtt accttgccga gttgtgccaa
     1441 tggtttgcgg attatccgtt agcgattgaa tttcgccacc caagctggca tattccggcg
     1501 gtgtttgata cctttgcgaa aagctctaat ctgatttggt gtaatgtgga ttacccacaa
     1561 aatatcggct taccggcatt tcactttttt gccaaccaac gtactgctta tttacgctta
     1621 catggtcgta acccgaattg gtggaaaggg cagagtgcgg cagaacgcca cgattaccgc
     1681 tattcagacg aggaattaaa aggcttggca gaattgcttt ttagccgcag aacggaattt
     1741 gacaggctct atctctactt tgaaaacacc actaaaagcc acgcctttta taatattcaa
     1801 accctaaaag gctttttggc ggaaaaagga tttcaaatca aacctgtgcc ggagaatttg
     1861 cttgggcagc agagtttgtt ttgatgggta atttacattg ttgaggagta aataaagggc
     1921 catgccattt agtaatggat agcccttaat ctcaatattt accattcata tgtaactttg
     1981 aacgagatat aacgctgttt atttagcgta ttccccgtta agaaacctgc ctgaattcct
     2041 gcgtttatat tgcgataatt agcggatata cctgcttctt gtttaaaata acgccccatt
     2101 tgctgctcta tttgtaaatt attgaaacgt gttcttaatt tcccatggct tgcatcaaca
     2161 aaatagctac ctatttccgg ttttatttgc aggccattta gttcaagcgt tttattaatt
     2221 gacagaccag cttgaagagc gagaagatca acagcgtttg tttcgatttt tgcatctaca
     2281 agttgattac ctgctgaaga taaatgatga taacgggcgc taaatgatgg gattatattc
     2341 acgtttgcag attcccatgc tttaccgata ctaagccctg ttacaaatac tgaacgatct
     2401 aattttactg tatttgcttg ataagttaag cgattgcttg ctttcccaaa accggtatct
     2461 atcgcaacaa aaacaccatt ttgccatgtt ttcttcgcat acattgatag cattgttaag
     2521 cgacctttac cgctataata atggtcaaaa ttatttctcg cttttgattg agataatatc
     2581 ataccaagtg tgacctgatt atctaaggct ttctctaatc ctagatggat aacgttcgca
     2641 tcttggttaa atccatatcc ggcagagcga tgctctgtcg tttgataatc tgttttagac
     2701 catactttcc aattttgggt cccttccgaa tgaattacat gatctaatgc tgtagttaca
     2761 cctaatacag cattagtttg cgcagttaac tcggatgcga tcaaatgtgc atttccattg
     2821 tatttaggta atggctcagc ggtttcaccc gatccttttt ctgaatgctc aatgtctcct
     2881 gtgtattcag gtaacggatc agcagtttca cccggccctt tttccgaatg ctcaatgtct
     2941 cctgtgtatt caggtaacgg atcagcagtt tcacccggcc ctttttcaga atactcaata
     3001 tccccggtgt attcaggtaa tgggtcagcg gtttcacttg gccctttttg agtaatttta
     3061 ggttcttcct ctttctctgg attgaggtaa tacacattcc cttctttgag taaatggtag
     3121 cgatattcgc ctgagtgcac ttcatttgct aaattaagag aaaagtcttc ttcatttgtt
     3181 gtttcaaata atttaatctt ttgggctgaa tgggcgctac catttgtgct aatttctatc
     3241 gaaaattgac cgcttgcggt gtctttaatt gtgactatat cgctcgctgt tttcgtcaga
     3301 tcagcgacaa atttaaatat gccgttccct gtgagggtat tagcggtaag cgtattctgt
     3361 ttttgggtcc cttccgaatg aattacatga tctaatactg tagttacacc taatacagca
     3421 ttagtttgcg cagttaactc ggatgcgatc aaatgtgcat ttccattgta tttaggtaat
     3481 ggctcagcgg tttcacccga tcctttttct gaatgctcaa tgtcgcctgt gtattcaggt
     3541 aacggatcag cagtttcacc cggccctttt tcagaatgct caatatcccc ggtgtattca
     3601 ggtaatgggt cagcggtttc acttggccct ttttgagtaa ttttaggttc ttcctctttc
     3661 tctggattga ggtaatacac attcccttct ttgagtaaat ggtagcgata ttcgcctgag
     3721 cgcacttcat ttgctaaatt aagagaaaag tcttcttcat ttgttgtttc aaataattta
     3781 atcttttggg ctgaatgggc gctaccattt gtgctaattt ctatcgaaaa ttgaccgctt
     3841 gcggtgtctt taattgtgac tatatcgctc gctgttttcg tcagatcagc gacaaattta
     3901 aagatgccgt tccctgtgag ggtattagcg gtaagcgtat tctgttttgt tgcagttgga
     3961 gtgtttaaat ggatttggct accatttgcg acattcaaga catctacaac agtgttggtt
     4021 aaggttgtcc ataccccaga aagattaacg gttgaaccat tggcgccatt aaatattcct
     4081 gttgtactca agccggttaa attagcaata cttccatcat taagttgaac cttagtggac
     4141 tcctgagctg taattgagcc agttaagtct gctttactgc ctaacgaaaa tgttgaattg
     4201 tcagctaaag agacattgcc agccactttg gttctctccc agctgtttaa atcagtctct
     4261 gataaagtag cgttaatatt acaggttgct acgcctgtgc ggtcagagcg aatacatgct
     4321 tgtgaggtac cttgggtaaa accaatttga gcggtagcat tatctgttaa attaaaatta
     4381 ccgttaatat tggcaacatt acggctgatt tcaagcttgc cattgtcttt agctgcaaag
     4441 attgttgcgt tgaacgatcg gttgatccag tcgccttcaa tcaggacttc tttattcgct
     4501 actttatcat aggcatgagg tgttggacgg cccgataaaa tgatttcgct tgtaccttct
     4561 gcagaaagcg taccgttgag gttcgttccc ccagtgataa gcaatttgcc gtttgctact
     4621 tcattactgt atagaaccat taaaactaag aaataaaaga ataataggaa tttggcggaa
     4681 tttttcggga ataagtgaaa cagaagtatt gaaaacaggg gctttgcggt gcattgcccc
     4741 tttgttttat gtagaaaaaa gtaaaaataa tgaattgcga aaaaatggct aaaactaacc
     4801 gcttgtacca ttaaaacaac gaaaaagtaa aaatgattta agtagcttta aatggcgttt
     4861 aaatcatctc gtgtccaatg tacacaacct gcccgataac ctcaaaatct aacgtttcat
     4921 caaaaatcac atcaataggg ctgtatagct ctttattatc gctgattaag cgaataccgc
     4981 ccataatgcc ttgcacacgt ttaacccaaa gctgctcgcc ttggcggaac acataaattt
     5041 taccgtcttt aggctgggtg gtggctcggt ttaccaatag catatcgcca tcactgatgg
     5101 ttgggtacat tgaatcacct gaagcggtaa taaatgcaag atagctcaaa tagaagccac
     5161 gcacatctag ccatcgttta cttagcccaa tatagtcatc aggggcatac acctcgctat
     5221 tgaaagcacc aaagcctgct gaagcctgta cattataaaa cggcacacgt tccatttcat
     5281 ctgcagtttg ggctgccact aatgttctag gctcttcttt tactgccgtt tgagcaaagc
     5341 ctaactcttg ctgcacgctt attgggagag agctgtagtg gtattcaaaa gcaacccccc
     5401 tttttccctc tatttgtctt ttgagccaat tatttttggt agctcttctt gttacatttg
     5461 atggttgttc aggtaaattt cccattccag ctacttcaga tgccgtaaac cattcctttc
     5521 taattataga attcataaaa ccccttttga attcaagtgg aattcaaaaa aatttacaaa
     5581 caaattctct tgaaaatcaa ttagttaatt aaataaaacc gaaaaacact aaattctttt
     5641 gaattctctt attgaattca tttggaattc gctatattat tcgttctgta gttaaatgat
     5701 ttacatcatt taagtagttt ctaaaactaa ctttcaagga tctcacaaaa tggcaagaaa
     5761 taaaagagtt cgagatatga gtaatttcga aattcgtggt gagctgatga aaatcggtaa
     5821 gtccttatct cagttaggta ttgagaatgg attagcaaaa accacagttc gtaatgcgtt
     5881 agataagcca tatcccaaag gggagcaaat tattgcaaaa gcgattggta aaacaccgca
     5941 ggaaatttgg ccctctcgtt acgaataagg agcttttatg aaggaatggt ttttagccaa
     6001 agaaatcgca ggattatctg gagttccaga attaccgaat agcgtttcaa gattggcaac
     6061 caaagaagga tggcaaaaac gacaaattca aggtgtaaga ggtgttactt acgaatacca
     6121 cctcaccagc ctacccattg aaacccaaca acaacttaga ctaaacgccg cccttgcggt
     6181 aataccgcaa gcggcagaac tacaacccaa acgagacgac cccgcactta tcgcaaggct
     6241 caataatgcc accgataaag gcagggataa agcaaaaggc aaggcagaag cctgtatgca
     6301 gttgcaagcc tttctcgacc aagggtttag ttatacgcag gcagaagcgg gagcggcaac
     6361 cgctaaaaat gtgtcgcaag gttctttaaa aaattggtac tacaaagtga aaggtcaccc
     6421 cgtccattta tggcaggcga tcttaatttc cgaaagcggt aggagtaaaa agccgcagct
     6481 gaaagccaag attactgaag aagcgtggga ctgctttttg gcggattacc tccgccctga
     6541 aaagccggat ttacgggcaa gttaccgccg aacccaagcc atcgccaagc aatacggctg
     6601 gcaaatggca agtttacaga ctttccaacg ccgagtgttg gcggaagtgc cttacgaggt
     6661 gattttgctc aaacgggaag gagcaaatgc ggttgccaag ttagtgccgg cactacaacg
     6721 cacggtgaaa gatattctcg caggagaatg gattaacggc gatggctatc aacacaatgt
     6781 gtttgtgaaa tggcacaccg gcgagattgt ccgccctaaa acgtggtttt ggcaagatgt
     6841 tcgcacccgc aagattttag gttatcgaac atcgatttcg gagaatactg acagcatacg
     6901 ccacgcatta atggatgtga tttttaacgt aggcataccg aaaacgctca cgctggataa
     6961 cacccgagca gcagcgaata aggcaatgac cggtggtatt gaaaaccgct accgctttaa
     7021 acataccgag ttagacccga aaggcattat gccgatttta gggattgatg tgcattttac
     7081 ctcaattctt tatggtgaag gacacgggca agccaagccg attgaacgtg ctttcgggcg
     7141 aggcggaatt ggcgagaaaa tagataaacg ccctgaatta agtggtttct atacgggcag
     7201 aaatgcacaa gaaacccctg ataactataa cggcggtaaa gacggtgtgg attacaacac
     7261 attcttgaaa gccatagcag cagggataga agagtacaac tctcaaaccg aacgccatac
     7321 cgaaatgtgc cgaggagaat tgagttttaa ccaagtttgg gagcgggatt accacccaag
     7381 caacgtaaga caagccagcc ctgaacagtt gcggttactg ttcttacagg cagaaacggt
     7441 cagcattaaa cgcaacggca gttttacgct gaaagcagcc ggcaaacttt acggcttaac
     7501 taacgtttac tgggcagaaa gcctgatagg gataacggat aagaaagtag tggcacggtt
     7561 cgaccccgat gacttacacg gcaacgtgta tgtgtacaac ctagaggggc aattcttagc
     7621 cgaagcggtt tgccgagagg cgaaaggatt tggtgatacc agtgcaagcc gtgagcaagg
     7681 tcgcttatat cagaaagtag tgaaaagtgc caaagctcaa gccgaagccc ttgaattact
     7741 ggaagctcac gaactggcaa gcctcgcccc acaagtggac gtgcctgagc cgatagagaa
     7801 gaaggtaaaa gaggtgctgg tgaaagaaga ttttatcgtg gatttcaaca ctgtgcgtaa
     7861 agcaacggtg gtggaagaaa ccgaagagat cagtattttt gatgaagtgt tactgaaaca
     7921 agagaaacaa cttcgcatag tggaataact agcggtcagt ttttgcaaaa aatttgcaaa
     7981 tttttctaaa aaaacgaccg cttgaaatga gatttaaaca gggtttaaac gcctttaagg
     8041 agtaaaaaat gacattaaca gggcaagctt accgagtgat tttcaaggaa ctcaaagagc
     8101 aacaagagag tgaatttgaa caggctttca agaaagggat tgccaaaatt cagcagaatc
     8161 aaactcagga atccattctg gtaaaccgtg atatgtttca agctgaaaag ctaacactgc
     8221 tttcacaaaa gttggaagcg gttgagcaat tacttcgcct tgaattaaat cagccgaatt
     8281 ttcaagcaaa acttgaagac tacgctgacg cattacgaac aaccatttta agtaaagtag
     8341 ccaaataaga gctttagact cttgaaggct taacatctga gccacttttt ttactgtcgg
     8401 cattataccg tcaagatcat caatagaagt taaatgatga aaagaggaat ggtattcagc
     8461 taaacaaaaa ttgattgtac taagtttaaa caaagtaatg caatttttca tcacgatggc
     8521 atttgctcgt tttaacttaa aaccagagaa ggttgcacga gcagaatcaa gggctatttg
     8581 atgaatttca tcatctgtta aagaacgaaa gagcataaaa acctccaaac aaaaacagga
     8641 caccattatg acattaatcg accaaattaa acaacacatc gccgaaagcg gcagttctca
     8701 ggggaaagtt gccaaagaag ccgggattaa tgccggggcg ttatcggctt acctgaatga
     8761 aaattacaaa ggcaataatg aagaacttga agcaaagctg attgcttatt tagagcgtat
     8821 tgaggcgaag aaaagagagt ttgtcgaagc tccgagtttt attgaaacca aaaccgcaaa
     8881 ccaaattttc ggttcgttac gatttgctca aaatactggc gtgcttgcca ttattcacgg
     8941 tgcgagtggc gtgggtaaaa cacaagcggc tagagagtat cgtaaacgct atgcgaatgt
     9001 atggttggtt accgcaagcc cttctcgttc ctcattaagt gaggtgttat atgaaattgc
     9061 tcttgaactt ggaatgaacg atgcaccacg ccgcaaaggg acattggctc gtttaattgt
     9121 tcgcaaaatg aaaggcacag caggtttatt aattattgat gaagcagacc atttacctta
     9181 cgaagcctta gaagaattaa ggctgttaca agaagaagta gtgaatgaaa gtgacatcgg
     9241 agctggtgtt ggcttagtgc tgattggcaa tgacaaagtc tacacccgaa tgaaaggcgg
     9301 aattaaccct gctcacgaat atgggcgact ttggtcacgc agtgccaaac gcaccagtat
     9361 tcagaaaacc aaaaaagaag atacccaagc ggtagccaaa gcgtgggggt tgagtgaaaa
     9421 ccaagaagca ctcaaggtaa tgcaagccat tactgaaact ggtggcggat tacggattct
     9481 gacacacacc ttacgattag cagcgatggt agcaaaagac cgaatgattc ccttaactgc
     9541 cgatttaatt cagctcgcac gtaaagattt actctcaaca ggagattaac aatgagaacc
     9601 ccaaaaccca aacacagttt taaccgcacc aaccaagcag cgtgccgttt tttaaaacaa
     9661 acccaaaagg caattatccg cttaaacagt cttggcttta cggtgctgaa tattgatttt
     9721 acccgtatta agccccgcat tgaggtggaa atcggcaata acaagcacat tgctcaaggc
     9781 ttgatttatg agggtaaagc ctaccgctac agcttcggta aaagcgaaga tttaggcaga
     9841 tgggaaggct actacaccat gctggaaggt atcagagtgt ggtggcgaca agcagcaatt
     9901 cactaggaga aaaacagatg aaaaccttag cactattaac cgccctttta gggcttgtag
     9961 ggtgtgattt agtccaaagc aaacaagcta aaaattacat cggcaacaat ttaactgaaa
    10021 tctgtataga taacgtggtg tacctgattt attccggtga taaaaaaggc ggaattaccc
    10081 cgaaagtaaa taaagatttt tacccttata cctgtacaaa caaagaggaa agacaaaatg
    10141 gcaaaacccg atgaaacacc cattacaaaa gcagaatgtc aatctcagct tgcagagttg
    10201 ggggtgcaat ataaaaaact gccaatggca attacaagac atatttgtaa tgccacaacc
    10261 aatatacacg gcaaagttat aaaggtcagt gtggtagaga gagttggata tggcgtccag
    10321 attaccgcac aaggtaacga gaaatcttgc ttagtaacct atgaagcaat gttaggtatg
    10381 gcagaagcaa tgggattatt tgatgaagtt aaggagcaaa ataatgacta aaaaaacaac
    10441 cagagtaaaa agtgcaaccc aaagtgcgat ttatcaaagc cgagatgaag tgcaagtggc
    10501 tataaaaatc attggtgata aacagcgtga attgcaacgt ttagctacgg caatgaatga
    10561 tgaattagct gctattagtg ccagttatgc tcaacataac cgctaccaac attatgccga
    10621 gaaagcggct gaagcagaac gcaaaggcaa ctataaggaa gctcaagacc attgggaagt
    10681 tgcaaaatta tcagcaaaaa agaccgctaa ccgagactgg gcggaacaac gagcggaatt
    10741 ttgtaaacgt atgcacaata aaccgtttta gggggaagtg atgacagaaa ccgtaaaagc
    10801 acaacttaac agccaattaa atgaggcaat tatccagttg atacaagctc aaaagtattt
    10861 aaaccaagat gatgccattc gcagtggagt gtatatcggt acggtgcagg atttattgcc
    10921 aaaggtgcat ttaaagttat taacggcaaa tcgtaaacat tgatagagat gaggtaaaaa
    10981 tggctattac agaagaacaa tggaaagaaa ttaagcaaaa gctagatagt atcataggga
    11041 gagttaaatt tagatacaaa gagcatttac tcacggtaga tgttgtacaa attaagcgat
    11101 ctttaaaatt ggctgtgtat gttgatgggg aaattgatgg agcttggaca aaagaaggac
    11161 acgaaattcg cccttattta gaagaagttt ggtatcgcaa agaacgccct ttttttaatg
    11221 ctaaggagaa gaaagagtat agaggtttaa tgagcaaaaa aaaactgaat gaaaaaatag
    11281 taacctactc acctatgttt ccatcaccaa ctgcactgac tcgtcaatat aagaaactag
    11341 atggcttaga gttgattgaa gtgatttaaa acccatttac agcccattta accccgagtt
    11401 atttaaagga caacaaaatg aataaaaacg aattttttaa gaaataccac ctagagggtt
    11461 cactagctat tttggatttg gatgcaaatg acgatcaaag ccaattctgt taccaagcat
    11521 tgttggagta tagagaactc ccgttagaca catttcttgc agagtactca gatgatgagg
    11581 atatgacaga gtcagagttt attgagttaa tgcttgatga attagagtgg attatccgca
    11641 gagctaagaa tgctaaacgg tttgttaaaa aacgacaaaa aacgatgagg gaggaaatta
    11701 tgtaacagct caacccaacc aacgccacat catccgtcta gctgagaaac aggtgtggcg
    11761 tttgttttaa gtgggcaatc aaaagcgata acgtttttaa ttgtttattt atcggagtaa
    11821 caaatgacag acaaagccaa gctaatccag cttatccata ttgccaagtc gcaactagca
    11881 atggacgatt taagctaccg tgaaatgctg aaacggctca ccaataaaac cagctcgacc
    11941 aaatgcacgg tggtcgagtt gcacaaagtc ttacacgaac tgcaaaccaa aggagcaaaa
    12001 gtaaaatatt ttgccaaacg cagcaaaaaa ccgaccgctt acagcccggc aacaggggaa
    12061 acagcggtaa aaagccaaat tgctcataaa atccgagcgg tatggataac aatgggcaaa
    12121 cacggatttt taaacgaccc aagcgaaaaa gcattaaatg cttatatgcg taaggttatc
    12181 aataataaaa ctagaccaac aatattatta aatgtctctg cattagaggc tggagatgca
    12241 agttatatgc ttgaaatcct caaaaaatgg cataaacgtg tgatgattaa agccctaagc
    12301 aaaagttgca cagttgatcc aaagataagc tacgatgaat tagtggagtg gtacaatgaa
    12361 gttatgtaga tgcccgattt gccacagtga tatacaccta gaagctctcg tagaagatga
    12421 agccgggcgt gaactactcg gcaaaatcag ccaattaaca cacggtgttg ctcaaccgat
    12481 ggttgcttat ttaggcttat ttaaaccgca aaaaagtaac cttaataact caagagcctt
    12541 gaaaatactc aatgatgtat tagcactcta cccttgctca ttgctactgg ctcaagccct
    12601 atctgaaacg gtagtaagca tacgcaaaaa acgtcagcaa gcaatggaaa acgggcagaa
    12661 aattgaaccg ctaccgaacc ataactattt aaaatcggtg tatgaaacgc aaaaagtcca
    12721 ttttgctgtg gtgagaaccg gcaaaaccga acgcgaaaca atgaaagccg aactggaaaa
    12781 aacacgcaat gcgattgcct atgtgcaacg gtttgttgat ttaggacgtg aagaggttgt
    12841 taaaaatagc cctgagtatc agatttggtt agctcataaa cccaaataac acaagccacc
    12901 aaaaggtggc tttttaactt tttgaaagtg cgataaaacc tgatttaaac aggctttaaa
    12961 cgggttttaa gattgagttt aaaaaaatat ttcacaaaat aagaatggtt ttttatacaa
    13021 taaccgaaac ggtttttgga taggaaatag acaatggaat tagtggatat ttttgaagaa
    13081 aaagccccgg aagtgttaac cgatttagcc aaacatattg aagtcgcatt agaagaaaaa
    13141 tgccaattta gccccgaaaa ggcaaagcaa atcggtattg atattgccca acaaatggct
    13201 caaaactggg gtggcgaggt aatttatatt cctcgcaatt tgttgatggc tcttaatgag
    13261 cgagatatga aaatttacga agagtttaac ggtaacaatc accgtgaatt ggctcgtaag
    13321 tacaatgtat caatgcagtg ggtatataaa attgtgaaga aagtccataa ggacgaagtt
    13381 gcccgcaggc agatgagtat gtttgagatg taaatcattt tttcccagcc ctgatcattt
    13441 tcagggcttt ttgtgtatta aaagaacgca gtctttaaac ccgtttaaaa tcaatttttc
    13501 agcaaattct ttaaactcct tttaaagcaa ttttaatagg agtgtgctat gtctcaaaat
    13561 cttacctttc gccaaatttt tgaccgttta atcggacacg agggcggcta tgtcaatcac
    13621 ccacaagacc cgggcggaga aacccactgg ggaattacca aacgtaccgc ggttgctaat
    13681 ggttatatgt atgcaatgaa gaatatgacc cgtgagcaag cctaccaaat ctacgaaaaa
    13741 gccttctggc aacgttatcg ttgtgcagag cttaagccag cagttgctta tcaatttttc
    13801 gacgcagtgg ttaatcacgg ttttggtaat gctagtcgta tgttacagcg tgcagtaggc
    13861 gtagcggatg atggcattat tggcaatatt accctgcagg caattaatgc aaaatctgaa
    13921 agcgatctct taatgctatt taatgccgaa cgtctgaagt tttacacaaa attacgcaac
    13981 ttccccactt ttggtaaggg ttgggtcaat cgtgtggcag gcaatttaga atacgcagca
    14041 caagataatg aggtttaaaa atggcaaaac tatttaaaga actactctca accaatggca
    14101 cattatccac cactagcacc gtgcagtttt ttggcacatt gtgtgtattt ggtttaatgg
    14161 tttatgcagt aattacccaa caaccttatg ccgaaagcct attaaataat gtgttgattt
    14221 atctttttgg ggcaactacc gctaagggtg tagtaaccag ctatcaagcc aaaattaaag
    14281 ggggtatcca taaacaacaa agtacattta aagaggagga gggatgttaa tgcaacaaat
    14341 tttaggcgtg attggcgtca tcgcagcaat attgtcttat gctttgttta aaagctggca
    14401 actaaaacgt gagcgtaaaa agaatgccat attacaagct gaaaaacagc aacaagcggt
    14461 tgaaattgag caaaaaaaag cagaggtaaa atatgtgtat atatcaaaga aaaacaatga
    14521 aaaagttact cgcagctctg ctagcgatat tgatgacaag ttgcagcaac acggttactt
    14581 ccgtgacaac aacaggttgc acggcgttca gtctgattta tccgaaccgt gcggatacgc
    14641 tggaaacgaa acgtcagatt cttgctcata atctgacttt tgaggaaatt tgccaaaatg
    14701 aaatacatta aaaaattacg taaacgctgg caaatttggc gttttttaaa aaatcatcct
    14761 gaggttgcac aacgccgaga tttattagcg attgccatta aacagggcgt agaaaaccct
    14821 gtagggcgtg caagacggag ggagccataa tggatttatt tacagtcttt tgggagtcta
    14881 tccgtgctaa ctttggtttt atttctgcgt tactcaccat tgttgttacg gcattttggc
    14941 tcaagctaga tagcaaatac gccaagaaaa atgatcttgg tgaattgctg gaaattgcca
    15001 aaagccacga taaccgctta accaccttag aaaccaaggt agaaaacctg ccaagtgcgg
    15061 tggacgtgga aaagctgaaa accttagtca ctgatgtaaa aggcgatacc aaagccacct
    15121 caaagcaggt ggatagtatc agccaccaac tcggcttgtt attagaagca aaattaaagg
    15181 aatgatatgt cgttaagaga tttactcact caagaccaac ggctggtgat tttacgctca
    15241 ttagcggaag ccggctatga tgccaatgag tcaattatta gtgattgcct agatttatac
    15301 ggtcacgata ttagccgtga tttagttcgc acccatttaa gttggcttga agagcaaggc
    15361 ttacttacct tagaacgttt gcgagatggc tatatggtgg caagcattac ccagcgtggc
    15421 ttagatgttg ctcaagggcg tgttgtggta agtggcgtta aacgccctcg ccctaaaatt
    15481 taaacggtat ttaaggagcg tttaaatgag cgagaaaaat actcgtggtc gagcatcaaa
    15541 agtcgattta ctgccgccga atattaaaac ccaactggcg atgatgttgc gtgataagca
    15601 gtactctcag tcacaaattt tagaagaaat taatgactta attcgggatt gtggcttgcc
    15661 tgatgaaatg ctgttaagca aaaccggatt aaaccgctac gcaagccgta tggaacgctt
    15721 aggggcaaaa atccgtaatg cccgggaagt tgccgaaatt tggactaagc aattcggcga
    15781 aatgccacaa accgatattg gcaaaaccct aatggaaatg gtcaagcaaa ttgcctttga
    15841 aacctcattg aaattaggcg aacaagaagg cggtattgag ccaaaacagc ttgctttgct
    15901 ctcgtctgct attcaacgtt tagaacaagc cgaaagtttg agttttaaac gggaacaggc
    15961 aatccgtaaa gaggtcgcac aacaagccgc tgaaaccgct gaaaaagtgg tggtgcaagc
    16021 gggtttatcc gctgaaaccg tccgtacgat taaagagcaa attttaggaa ttgcataatg
    16081 gcactactca ataacagacc gctgaatgaa ttagcccctg aatgccgttc gtttttagag
    16141 agcattcacg cttttaatcc taacgaactg ttattgggct atcaaaagcg gtggattgcg
    16201 gacgacagcc aactcaaaat tgccgaaaaa acccgccgtt gcggtttaac gtgggcagaa
    16261 gcagcagata acgccttaat cgcaagcacc cgcaaatcag acggtggctc tgatgtattt
    16321 tacattggct caaataaaga gatggcacgt gaatatatcg atgctgtcgc aatgtgggca
    16381 aaagccttta atcgtgtggc tggagaaatc caagaagaga tctttgaaga cgaagataaa
    16441 gacatcttaa cctatgtaat ctacttctca tcgggtttta aggtcaaggc attatccagt
    16501 aatccgaaaa acctgcgtgg tatgcagggc gtggtggtga ttgatgaggc ggcattccac
    16561 gaatacttag cggaagtctt gaaggcggcg ttagctctca caatgtgggg ggcgaaagtc
    16621 cgtattattt cgacccacaa cggctcggaa aatctgttta atgaactgat taccgatagc
    16681 cgtgccggcc gcaaacgcta ctctgtgcat acgattacga ttgaagatgc ctgccgtgat
    16741 gggctatatc aacgtatctg ccaagtagtg acgaaaaaaa cgtggtcacc ggaagcagaa
    16801 aaagagtgga ttgaaaacct actcaacgac accgccagcg aagaagacgc attggaagaa
    16861 tacttctgcg taccgaagtt cggctcaggc ttatggctct ctcgtgcttt gattgaacgg
    16921 cagatgagcg aagccacgcc tgttatccgc tttacggcaa aagatggttt cagccttgtg
    16981 gctgaaccga cacgctatgc ggagattgcc gaatggtgtg aattagaact tgagccgata
    17041 ttggcaaccc ttaaccctga tttgttgcat tttatgggcg aagactttgc acgcagcggc
    17101 gatatgacct ctctggtggt gcttgctcag caacaaaacc ttgttcgtaa agtcgccttt
    17161 attgttgagc ttggcaatat gccctacaag caacaagagc aaatcgtgat gtttatgctt
    17221 aaacgcttac cgcattttgc cggtggggct tttgacgccc gaggcaatgg tggctattta
    17281 gccgaagctg cacgagatga atttagcacc ttgattgact gcgtgcagtt atcggaaaaa
    17341 tggtatcggg aacacaccgc cccctttaaa gcggcattgg aagacggcga gctggaagct
    17401 attccaaaag atgcggatat tttggctgat ttgcgttcat ttcaggtggt gaaaggcgta
    17461 ccacgcatac cggataaacg cactaaaagt gcagatggga aaagcaaacg acacggtgat
    17521 accgcaattt ctttgctgtt ggctcattat gccagccgac aattagtgca aatgccagtt
    17581 gagccggtat cacgccgtcc acgccatagc caacaattaa cagaaggata ttaacaaatg
    17641 agatatgtac cactttgttt aatgatgtgt gcctcattct acctatttat taacaacatt
    17701 gacggctggg gttggggaat tcttttatct tttattttag cgagtgcagc ttatggcaaa
    17761 gaagaataat ttaattagtg aaattgccac ccgtgccaga agtattgatt actgggcatt
    17821 tggctattat ctgccgaacc ctgacccgat tttaaaacga atggggaaag atatttccgt
    17881 ttaccgtgag ttgttatcag acgggcaagt tcgctcaggc gtacgccgtc gaaaagcggc
    17941 gattaaaggc ttagagtggc gaattaccac cactaacaat gagaaagtgg acgagcagat
    18001 ttatcaaata tttaaccgct tgccgatgaa taacctgatt accgaaatgc tcaatgcagc
    18061 actctatggc taccatatca gcgaggttat ttgggcaaaa gagggtgagt tatttgtgcc
    18121 aacagaaatc attggtaaaa agcctgaatg gtttatgttt gacgacgaaa acctactgcg
    18181 tttccgtacc aaagaaaact gggcggacgg cgaattatta ccagatgata agttcttgct
    18241 tactacgcaa gaagccaccc aagataatcc ctacggttta ggcgatttat ccctctgctt
    18301 ttgggcggcg acctttaaaa aaggcggttt caaatactgg ttggaattta ccgaaaaata
    18361 cggctcaccg tggttgattg gcaaacaccc tcgccaagct cacaatacag ataaagagcg
    18421 actagctgat gccctagagg gaatgattag ctcagcgatt gcggttatcc ctgaagatag
    18481 ttcggtcgaa atcaaagatg cagcgggcaa atctgccagc accgatgcct ttgaaaagtt
    18541 cctcaatttt tgcaaggcag agatcaatat cgccctactt gggcaaaacc aaaccaccga
    18601 gcaagaaagc aaccgtgcca gtgcacaagt aggcttggaa gttatggaag atattcgctc
    18661 ggacgaccaa gcgatgattg aagccacctt caacaccttg ttgcagtgga ttgtgaaata
    18721 taacttcaat gtggaacaac tgccgaagtt tgaattttac gagcaagaag ccatcaacac
    18781 cgaccaagtt gaacgagaca gtaaactcca cgcaatgggc gtgcgttttt ccaaagccta
    18841 ctttgagcgt gaatatggct ttgaggcggg tgatattgag gtgcaagcgg tcgaaaaagc
    18901 ggaaaaatct gcaaatacca cagaatttag cgaaggacaa cgccgaaccc aagtgccaat
    18961 gcctgtgcca attttggaac agctagaaca cgagggagaa gcaatggtgg acgattggct
    19021 acaaggggtg agagataaac tcgctcaagc agatagtctg gaggatttcc aaagtcagct
    19081 agatagcctt atccctgaat taagctttgc cgaatatggc gagctgttgg cgttaggctc
    19141 aacggtggca gagcttgcag gcagacaatc ggtaaacgat gaaattaagg caaaacgcaa
    19201 tgcgtaaatt tacctttaaa cagcaagccc aatattttga gaaaaaactc aatctccgca
    19261 cgaatagcta ccttgatatt ctgggcgaag agcacgacta ttttttcgtg gtggctgggg
    19321 cgaatcgtaa tgaagtgttg agtgagtttc gcaaggcggt ggatgaggct attcacaacg
    19381 gcgaaacctt agagaacttt cgcaagcggt ttgatgaggt tgtggcaaaa acaggctggg
    19441 agtacaacgg cggacgaaat tggcgaacac ggattattta cgataccaat gtttacgggg
    19501 cgtataaccg tggacgtttg caacaacatt tagagcttgc ggaggatatg ccttattggg
    19561 aatatcaaca caacgacaac gcccaccctc gcccacagca cgtggcgtgg gacggcttgg
    19621 tgctgcgtta tgacgatccg ttttggaaat atcactatcc aataaaagcc tacggttgcc
    19681 actgcaccgt gcaagcgtta gatgattttg acctcaagca cgatggtaaa accgtcagcc
    19741 caccacctga aattgagttt gaacaaaaaa cggtaggcgt gcggacaggc aattcacgag
    19801 tcgtaactgt gccgaaaggg gttgatgtgg gttttacacc gcataatttt gataatctca
    19861 aagccgggag aacagcaaat gtagataagt tgctaatgca aaaaatggtt accgccgatc
    19921 ctatatttgc ctccaaagca gtcagtgatt tattggcaaa agagccggtt gcaaagttac
    19981 ataaccaagc ctttaaaaat tgggtaaatg atgttgccaa tgctaaagga agtgcattat
    20041 cccaagtaga gaatatgcag ttgttagggg tactacctgt tgcggtgatt gaacggttac
    20101 agcagatcaa tttagctccg caatctgcaa ttattgcaat aacaaaaggc gatatggtac
    20161 acgctctacg agagctaaaa caaaataccg gtattgccgt accgttggaa ttttgggaaa
    20221 agttgcctgc aaagttacaa tacccaaatg ccatcttgtt ggaaaaacaa caagcagaaa
    20281 ctacattgct ctttatctat gacgtagctc aaaccaaaat tgccgtaaaa gtcaattatg
    20341 atgcaacttt acaagataga cacggagcaa aaggacggca gaaagtctta ctgaatcgag
    20401 cggttacagg tagtttattc caagatacaa ctggattgaa aagttcaaag tatgaagtgc
    20461 tattgggaga aatgtagcgg cgaaggactc gaaccttata atgtaggtta aacctacaac
    20521 ctttaccaat taggaaacta ccgctacatt gtgtaaattc actatacccc taaattattt
    20581 tttaatcaat aggagaaaca aaatggcaga aaaacaactt tcctttaaag tagccgaaat
    20641 agtgccaatg tcagctatta acactatgcg gcacttaatt agtttggcat tacaagcgaa
    20701 ttatgagaga gaaaaatctc gtgcttgata atttcaatgg cttgtagttc gggaactata
    20761 ttatctgaaa caagccattg ttttgttgat gtcagacatt tagcgatgaa atccataatt
    20821 aaaccattag cagtagctcg ttgttccgga gtaagttgta accacgacgc ctctgtttgt
    20881 tggtctaatg tataaagaaa cataacggct tctttttctg ataatgggtc aagtggtaat
    20941 ttttcagctt ttgtgactaa ccattgacgg gaaagcaata aatcataaac cagttctatt
    21001 gcttgttctg catttaccat catattctaa ttccttaata tatcagggca acattaccca
    21061 atctatattt tactgagatt tgcttaatga tcaaaatcac cctcaacaac acccaagcgg
    21121 tcaaatcgtt acaacaaatt gcaaatcagc tggaacaacc tcgccgactg tatggcattt
    21181 taggggaaac cctaaagaaa attcacactg cacgctttaa agcggaacaa gaccccgatg
    21241 gcaacaattg gaaaccgtta gccagtagca cgcttgcttt aaaacgtaaa cgaggtaagt
    21301 ccactaaaat cttacggcaa gacggctacc ttgccgataa aacggcttac aatgttacaa
    21361 gtgataatgt tgagtttggc tcgcaggaag tgtatgcacg gttgcatcaa ttcggcggta
    21421 aagccggacg aggcaaaaaa gtgactgtac cggctcgccc gtggttaggc gtaggcgata
    21481 aagatgagca gttactttta cgcaaagctg agtggcattt agggcaaatt atcggtcgta
    21541 tgggaaaata accgcttaaa atcaaattta acgcataaaa acgcctctgt gcgtttttat
    21601 tttttattcg cctgattatt cgactcaaaa aatttaaacg ctgttaaacg catttaaacg
    21661 gtatttaaac ggtattgccg attgtgataa atcctcttta ttaaaacacc ctcgaaaaat
    21721 ctttaaacca gtttaaaagc taatttctct ccaaatcctt attattgccc catcacggag
    21781 gcaatatgac ccaaattgaa atttttaaag ccggtaagcg ttatgacgcc aacggcacgt
    21841 taattgacat tacccctgaa atgttgcaac agaccgttgc tgcctataac cccgaatttc
    21901 acgaagctcc gctggtgatc ggacacccga aatccaacaa ccctgcgtgg gggtgggtaa
    21961 aagccctttc ccttgagggc gatgtactaa aagcagaagt agatcagcta gacgctgagt
    22021 tttcagaaat ggtggcaagc ggcaaattca aaaaagtctc ggctgctttt tacttacctg
    22081 atagcccgaa taacccacat aaaggcgtgt tatcgctacg ccacgtgggg tttttagggg
    22141 caatgccacc ggcggttaaa ggcttaaagc aggtggaatt tgcagaagat gatgattttg
    22201 tcgtcttttc tgaatggggg gttgcaagtc tatttagtcg ttttcgtgat tggttggtga
    22261 gtgagttcgg gcttgaaaaa gccgataaag ctcttccccc acacgaaatt gattggctga
    22321 aagaagaggc aatgcgaagc caaattagca aacaggtaca cgccgaacag gcaacacctg
    22381 aaccgatttt taatgaaccc gcacaaccag aaggagaacc tgaaatgagt gcagaagaga
    22441 aggaagagct tgaacgctta aaagcggaaa acgagcagtt gaaagccgaa aaagcccaag
    22501 ccgaagcaga gaaaatggaa gcagagctgg aagctgaaaa agctggtaat gcagagtttt
    22561 gtgaaggttt agtcaagcaa ggcaaacttg ccccggtggc aaaagcggcg tttgtgcagg
    22621 ctttaaataa cctttcagta ttaaaagccg gtcgtgagcc ggaatttaat gagggcgaag
    22681 atgtggtttc acagtttaag tctgcaatgt cgcaatcacc acagattatc caatttggag
    22741 aatatgccac agcagataaa gccaacccac aagaagcgga agcagtggaa tatgctgaag
    22801 gcacagatcc agtttcgatt gaagccgacc aaaaagtgcg agcttatatg aaagcccaca
    22861 atgtaggcta cgctgcggca tttaatgcga tttatcgcta atgtttaacc cacaactgaa
    22921 aaggaatact aatgacaact cataacttac aaaaactgcg tgtacaagac cccgttttaa
    22981 ccgagctggc acaaggctat cacaataacg agctggttgc cgagacctta atgccgactg
    23041 tcgaaatcga aaaggaagca ggcaaaatcc caactttcgg gcgtttagct ttccgattgc
    23101 cttctaccgt gcgtaacttg cgtggttcat ctaaccgttt agatccagaa gatattgggg
    23161 cgattgatgt ggcgttagaa gaacacgatg tggaatatgc cattgattat cgtgaagaga
    23221 acgaagccat tttttcttta cgtcaatttg ccttaaatac cacccaagat gtgattgcgt
    23281 taggtcgtga aaaagcggtt gcaaccctcg ccttagatga aagccgttat gaaacagaca
    23341 ataaagtcac gctgtccggc accagtcaat tcagccataa agatgctgat gtgtttaagg
    23401 tatttgatac cgctattcgt gccgtgaagc gggctatcgg tcgtaaaccg aatgtgtgtg
    23461 tgatttccgg tgatgtgtgg gcagcgttaa aagaacatcc ggcagtggtt gaaaagctca
    23521 aatacagtca agtagcgatt gttacccctg aggtgtttgc caaactgatc ggcattgaga
    23581 ccgtaaaaat cggtgaagcg gtgtatgaag aaagcggtca gctcaaagat atttggagca
    23641 aagatattgt ggtggcgtat gttgctcctc gttcgaccgg aggcaaaggc acggtttacg
    23701 agccgtctta tggttacacg gttcgccgtc aaaaaggctt atttgttgat acttataaag
    23761 agagtggcgg caaaattgaa gtggtacgca cgaccgatat tcacaaacct catttagtgg
    23821 gtgcggcagc aggctattta attaaagctg ccgtataagc cacataccat agggtgcgtt
    23881 tactaacgca ccattatctc aaacacatta catagaggaa aaaacgatgg ctaaatttat
    23941 tgttttacat accccaattt tacacgatgg caaacgctat gaggtggggg cagaaattga
    24001 actcaccgct gaacaagctg agaacaatgc gatcaactta aaagcggtag aagctgttcc
    24061 aaaaccacct aaaaatgcct caaaacctga agatgagcca aaacccgaag gtgaacctaa
    24121 gcctgaagat gcagtgacgg aagatgatgt gaagctggct gaagcaaaaa ttgcagaagc
    24181 agagcaacca aaagggaata aaaaataatg tatgtgcaga ttgatgagct actcacggcg
    24241 tttagtcgca agattgtggt gcagctcagt aatgatgaac cgacagcgac cgagcctgat
    24301 ttaaagatgc ttgaaacggc agttaaagtg gcaaatgagc gaattgatgc agcgttacgc
    24361 tctcgctata ccttgccgct ggctgatgtg cctacgctta ttaatcagca cgcattaacc
    24421 cttgcccgtt attggcttta tgcccgccgt cctgaaacca aaatgccgga caccgtgaag
    24481 gaaacctacc aacaagcggt gaaagagttg gaacagattg caacaggcaa actgcattta
    24541 ggcattgctg aaagcagtca aattttagaa gacgatgttt tagccgataa cagtgaatat
    24601 gaagttcggg cgaacgctcg cattgatacc agtcgttatt aaagattatg tcagccacac
    24661 aaccgatttt agaagatatt tgccaacact tgcggcaagc attgcccgag tgggaggtgg
    24721 aactcttccc gaatgaccca agcacctatc atctctcgca cattaacggt gcggtgttga
    24781 ttagctattt ggcaagcaaa tttgagaaac ctcgcactac acaagcggtg ctgcaggttc
    24841 gccacgttca gattgcttta acggtgctga cccgtgattt gcataatgat gtcggggcgt
    24901 tggatttgct ggataaattg cggttattgg ttgtggggtt taagcctcaa aactgcggtg
    24961 agtgttggtt gattgatgaa ttttttaatg gctcaagcga agaaaccggg ctttggcaat
    25021 atcagttgat tttgcaaacc gaaactcagc aggtgcaaca ccaagcggtc gaaaatctgc
    25081 caaaatttgt aaccgctcat cttcgccgtc aagaccagcc tggttcgccc ggacttaaaa
    25141 cttaaatcgt agggtgcgtt tgctaacgca ccatcaacat cacctgattt tacggtgcgt
    25201 tggcaaacgc accctacaag gagaatatta tgacgtttca ccacggttct gaaaccgaac
    25261 gagtaaacgg tggctctgtg ccggtatcgg tggttgatag tgccattatc ggcattgtcg
    25321 gtactgcccc aatcggcgca gttaatgaat tgaccgtctg tttaaccaaa aaagattttg
    25381 ccaaattcgg cacgatttta aaccaaggct tcaccttacc tgatgccttt gatattttag
    25441 ctcgttacgc agcgggtaaa gtgtatgtgg tgaatgtgct tgacccttcc cgccaccgta
    25501 ctaccgtgac cgatgaagtc ctcacactgg atcgcaatac gctgaccgct caaacagcga
    25561 aaaccggcat tattgcggtg acgagagtga aagacggtgc aaacgtgctg aatgctgatc
    25621 ggtattcggt cgatctgcaa accggcgtgg tgacctttgt ctctgcaccg tctgccccga
    25681 aaattagcta tatctatgct gacccgagca aagtgaccga agaggacatt aaaggcgggg
    25741 tggatagctt aaccggtaaa cgcaaaggtt ttgaactatt gcgtgatggt tttaaccttt
    25801 acggtgcgga tgccaaaatc ttaatctgtc cggaatttga taaaaccgca agctgtgccg
    25861 ctgcccttgc cacccttgcc gaccaactgc acgctaaggc gtatattcaa ctgccgaaag
    25921 ggacgagctt atcaaaagct attcaaggac gtggcccggc agggacgatt aacgcctcgg
    25981 caagcaatga gaacgtacgc catttcttcc cgtatgcgtt ggggtcaagc aatactcttg
    26041 aaagccttgc gacccacgcc gccggtttgc gtatgttgat tgatgtggag aagggctact
    26101 ggttcagcac ttccaataaa gagctgaaag gggtgatcgg gttggaaatt ccgctgacgg
    26161 ctcgggtgga cgataagcaa agcgaaacta atctgctcaa tgcggtaggg attaccacca
    26221 ttttcaatag ttttggcaca ggcttccgct tatggggcaa ccgttcgtct aacttcccga
    26281 ctgtgaccca tatcagcaac tttgaagtcg cctctcgcac aggcgatatt attgatgaaa
    26341 gcattcgcca agccgaattg cagtttattg atttaccgat tgatgatgcg ttgctggata
    26401 gcttccttga aaccatcgac actttcctgc gtacgcagaa atcgttggta ggttattcgg
    26461 tgagcttaga ctatgaatat gacttggttg atgcattcag ccgtgggcaa gtcccgattg
    26521 tgtatgaata cactcctaaa attccgggtg aacgtatcag caataaatct gtgatgaccc
    26581 gtaaatactt ggcaaacttg gtgtcgcaac gataagggga aataaatgag tattgcaatt
    26641 aaccaaattg taaacggaaa tgtttacatt aacggcaaca gccaaatggg gcgagtgaat
    26701 gaggtcaaaa tccccgatat tgagtttgaa tttatcgaac acaaaggcct tggaatgcac
    26761 ggtgtgatta aattacctgc cggcacgaat gcgattgaag cagaaatcac ttgggacagt
    26821 ttttatccgg aagtgcgtgc ggtaatgctt aatccgtata aatacactca gttgcaaatc
    26881 cgctctaacc tgcaagtgtt taactcgcag ggcttggcag ccgaagagcc gatggtcacc
    26941 attatgaatg tgtctgccgg taaaatcggc ggcacagggc ataaaaacaa agaaaatgcc
    27001 gaatttgccg acacgttaaa tgtacattca attaaacaga ccgttgccgg tcgtgagctg
    27061 ttatttattg atgtgctggc gaatatctac cgtgtgaacg gggtggatgt gttgcaaaaa
    27121 ttccgcacca atatcggtta gtaaaatctt taaagccgtt taaaatcaat ttaaacaccg
    27181 tttaagtaaa ctcctttgtg aagttaaaca acaaccactc acaaaggagt ttttttatgg
    27241 ctaacgcagc tcaacaagta ttaaccaatc ttcgtgtaca aaccacttat aaattgcaat
    27301 accctatcgc tttaccagat ggtagagtta ttgaaacctt agatgtacgt cgtccaaaag
    27361 gtaaagattt tcgtttgctt gatgaaaaag ggtttgaccc tgaaaaagat ggcgtaaaaa
    27421 ttctacgctt ctatattcaa cagctcacca cacttgtgcc ggaagatatt gatgaactcg
    27481 atgctgccga tattaagggc ttaaataacc tattggaaga tatgcttatt gagggaaagt
    27541 ccgacaaata agtgaactag ataccgttta tgctgatctc gcttggtggt ttaagttttc
    27601 accaagcgag cttttagaat tggatttagt ggatattccg aaatgggtag aacagatgaa
    27661 caggcaaata aaagccggtt atgggcagat tttacggtag agccaaataa agggttcggt
    27721 ggctaaagta agtaaaatgg cgacgcccca agctagatac acaccaaccc acgccaagat
    27781 tgtcaaaatc aatccataaa tgagtacaaa actaacgccc tcgcttaggc tttgtataga
    27841 ttgaatcgca tctaaccagt agaagatact ccaaccgact aatgctaccg ctaacaacca
    27901 tttagcggtg cattctgttc ttgttacaca agcctcgaaa ccagcccact cagtaattcg
    27961 attatttaat ttagtcagca tacaccacct ccttaaactt tccctatttt acgctaagga
    28021 tttaaccaat ggcaaataat ttagctatcg gaatggtaat tggtgcaagc ctatcatcag
    28081 gttttcacgg tgctttttca ggagcaaaga aaaagctaga acaattagaa caggctgtcg
    28141 ctaaaaatca gcaaagccat tctaaattag gttctgagct tactgcattg cgtagtaaac
    28201 aagctactct ctatgcagaa atgagccgag ccagtagtaa aggtggtgct gggcttgctc
    28261 ttatgcaaca ggaatataaa aaaattggca aagaaattgc caatattagc cgagagcaac
    28321 gcaaatggag tacagaatta gaaaaatcta ttgctaagca aactaaatta cgcaatgcaa
    28381 tggaacgtca tacacaagct aaagaaaacc gagacgagct taaagggaaa ataattggaa
    28441 cggtagcttc tgcggcaatg ggtgtcggcg tgatgaaaac ctatatggag caagaagaag
    28501 ctgcgaataa tcttaaaatt tcaatgctga aagcagatgg ttctttcggg aaatttgaag
    28561 agattggcaa gattgctgac acattaggga cagatttacc gggaacaaaa aaagatttct
    28621 acaagctcgc aatggcatta aagaaacaag gtgtttctga tgatgtgctg accggagggg
    28681 cattaaaaac ttctgccgaa ctgaatgtct tgcttgatat ggatcaggaa agtggtggag
    28741 agtttttagc gaagtttatg gaatctcacc ggctcaatga aaatgagtta ccacaagcag
    28801 ccgattatct gcaacgagca atgtttgccg gcggtttaag taaagagcag atgtatgaat
    28861 cgatgaaata ctatgctcca aaactgaact caatgaaact tacaggggct gagaataccg
    28921 aaaaagtgct ggcgattgaa gcaatggcag gtcagcaagg cttagagggt tcaacctttg
    28981 gtacgggctt aaatatgatg ttaagccgta tgaataaagg accggaaatg ctcaaggcag
    29041 cagccaaagg aatgaaagcc gaagctcgag agatgatgga ggctgtcgga gtagaattta
    29101 atttctggga taaaaacggc tcattcaaag gcattgatgg aatgctggct gaaatgacga
    29161 aatttgaaaa aatccgcaaa aaattcggtg acaaaggtgt aggtttagtt gcagaagagc
    29221 tgtttggcat tgaaggtggg cgtcttgctg atattttggc tatgaaaggt caaaaaggct
    29281 tagatgaaat gctgagtaaa atgcgtgaac aagccagcct tcaagatcgc attaacctta
    29341 aaacaaatac attaggtgct gccctagaat ctttaggagg agcgtgggaa acctttgtgg
    29401 gggatattgg ctctgtcttt gccgaagata taaaagggtt ttcaaaaggc ttgcaatctg
    29461 cggtggaatg gcttgtgcca tttgtatcaa aacataaaga ggcgattaaa tttgtaggtg
    29521 gtttggttgc tggacttgtt attttacgag gcgttttaca agctggttct tttgttattc
    29581 aaacggtaac tatggcaatg aacgccctca aaattgcagc aatgaccaat cctattgtct
    29641 tagtcattgg agcaatcgca gcgggtgctt acttgattta tagccattgg gatagcattg
    29701 ccccttggtt tgcaaatctt tggcaaaatg tgaccgctta cttcaatcaa ttttggactt
    29761 gggtaaaagg tgtttggtcg ggcgtgacgg actgggtgtc ttctgtgtgg aatggtgtag
    29821 ccactttttt cgggcagtta tggactacga ttactagctt atttagtgta gcttggacaa
    29881 atatccaaac aatatgggga ggagcaacag cttggttctc tggtttatgg gatgggatta
    29941 agtctgcggc ttttggtatg ttcgacagta ttagccaaaa agtcagctct gttgtagcaa
    30001 gcattaagag ctttttaggc tttggcgatg atgtggataa aatgggggca aaaattggca
    30061 atgtagctac ccaagtcatc agcaaagaac actcaggaca actcagcaaa accgcagata
    30121 tggcagccaa tacagggttc tctagcggtg gtttcaccgg gcaaggcggt aaatataccc
    30181 ctgccggtat tgtgcataag ggcgaatatg tgatgacgaa agaagcgacc tctcgtttgg
    30241 gtgttggaat gctggatagc ttaaactatg gcggtgctat gggtttcact caacctgttt
    30301 tgcaaccgat tttaggtaag caaaaaggct tttttggctc actttgggac gatgtgaaat
    30361 tcggggcaaa ctttgtcggc aatctgcttg gcttaaatca aggcagcctg aaaacgcctg
    30421 attttaaccc aaatgcgacc aatgcaagcg gtcaaaattc gtcgattttt agcaattatg
    30481 aaccactcaa ccgaaatgcg gtaatacagc aggaaagctc gcaagcaccg cagatagtcg
    30541 tgaactttaa cccgacaatc aatgtaaacg gtaatgctac gcaagaggct caatcgcaaa
    30601 ttatgcagca gttgcaaatg agtgcttatg agtttgagaa attgctcaat cgtgtgctag
    30661 accaacgaag ccgtagagcc tattaggagt gatttatgta ttttatgtta ggcaatattg
    30721 cctttgagcc ggtgaattta actgaattta gcgagcaaca taccgccgaa tttgccgaac
    30781 acgctgtgct aaaaggcaaa cctcgtttac aagcgatggg cgaaaagcta aatgaactta
    30841 atttttctct tcggttgcac cataccatag gcggtgtaga gagccgttat caagccttgc
    30901 ttgaagccaa agcgaagcaa gcggcattgg cgttaatttg gggcagtcgt tataagggag
    30961 attatgtaat tgtcgatatt tcatcgacca cattgttcac cgatggcaga ggcaatgccc
    31021 tcgctcgtga aatgcaaatc agcctgaaag agtttgtggg cgataatgac ggtggtgtgt
    31081 tgggtgaggc gttaaatttt ggtggtggct cactgcttgg ctctattttg ccaagcggtg
    31141 cggtaactac cctatctcaa atcaaaacag cagtcagccg aggtgtggag ctttataaca
    31201 gcggcaaacg cttagttgac gaagtgcaaa ataccgtggc gattattcgg cagttgaaaa
    31261 atgacccggc aactgcattg gcatatttac ccgctgcatt agcgaattta gacggggcat
    31321 tggggcattt tggtaatttg accggctcat cggcgacctt gggcggtttg caggagaaac
    31381 taccggcagc agtggtcttt agcgaggaaa tcggcgagat ttatcagagt ttgcaaacaa
    31441 tgcgaagcag tttgggtaat gcctcggcaa atagctggga taattggttt acaccggcag
    31501 agagtgcatt gacagaggtc acagacagct ttgaccatct agcaaaaccg gtggcaaaaa
    31561 tgaccgcttg gattgtgctg cgtaccgatg aggaggcaga aaatgacaca gttgcttaaa
    31621 catatcgtca aacagggtga gcgttgggat aacttgagct atcaatacta cggcaatgcg
    31681 tttgaatatg gtcgaattat tgatgccaat ccgcatatcg gtttttgcga ggtgttgccg
    31741 ataggggtaa caatttatat ccctgtactt aacgttaagc caacgaataa tgaaaatatg
    31801 ccaccgtggc taagggggcg tgatgcgtaa tgtaccgaag cccgattttt cactgtttta
    31861 tgaaaaaacc aatatcactg ccgagattga gccacatttg gtgcaactga cctatatcga
    31921 ccatttggaa ggtcaatcgg acgaactcac tgtggaattt gaagacattc aaggcaaatg
    31981 gatacgccaa tggttcccga ctcaggggga taagctcaaa gcggcgattg gctatcaggg
    32041 cgaatttctg acggacatcg gtgagtttga aattgatgaa gtggaatata gttataagcc
    32101 ttccactatt aacttaaaag ctctaagcac cggtatcagc aaagccaacc gtactttaaa
    32161 accgaaagcc tatgagaata cgactcttgc tcaggtggtg gcaaaggtag cagataacct
    32221 gaaattaaag gtagtcggta agattaaggc tatcccgatt aaacggatca cccaatatca
    32281 agagcgggac gtggagtttt tggcacgcct tgcccgagag tatcatcaca gctttaaaat
    32341 cgtgggcaat cagctggtct ttaccgataa aagcgaactg gggcaaagtg agccggtgtt
    32401 aatgttggaa gaacgggata ctatttccct acgtttgcga gatcggatta aagacactgc
    32461 aaaggcagtg gaaatcagcg gctatgatac aagcggtaaa aaagtggtga aaaaacgcaa
    32521 aaaggcaaaa gccttacgcc cgaatataaa acaagctaaa tcagccagtg aagatacgct
    32581 gaaaatcatc acacgaggcg aaagtcaaga acagattgat gcacggggtg aggctgcgtt
    32641 ggctacgcaa aatgaagatc aggcggctgg tgatattgag cttatcggca atcctaaatt
    32701 ggttgccgga gcaacgattt tattgcgtaa ctttggcgtg tttagtggca agtatctgat
    32761 taaatcatca cgccatacga ttagccgtag cggtggttat actactcata ttgaggtcag
    32821 aatgttggag tttattgccg atgatttaat cacattaggt atggaggctg gcaatgcaaa
    32881 cgcataattt tggggcgact tatcaagaag ggattgtatc cgctgttgat ccgaaaacgc
    32941 acaaagtgcg gtgtaaaatc cctgcgctgg aagatttaga aactgcttgg ctgtcgtttt
    33001 tgaccccgaa cgccggtggc aatcagtttt actgtttgcc cgatgtgggc gagctggtgg
    33061 caatgctact tgatgcacaa ggcgaaggtg gctgtgtgtt tggggcaatt tataacgagc
    33121 aagacccgac accggttgcc gatagcgata tgtgggttaa gaaattcagc aatggcacgg
    33181 agatttcgca taatcgcaaa accggcgatg tggtagtgaa gactaacggg catttaaccg
    33241 caacggctca aggtggggct acgattaatg caaacaccgt agtcaatggc acattgcacg
    33301 ctaccggtaa aatcacctct gacgaagaag tctctgctcc tgttgtgaaa caaggcacgg
    33361 tagagcttgg cacacataaa cacggcggta gccctgaacc taataagtaa ttctttaaac
    33421 cagtttaaaa tcccttttaa agatagccct gtatcatcag ggctatgaac agacaaactt
    33481 ccattcaatt aacccactgg caacttgcac cctcaaccga tacagttgag gtggtgcaag
    33541 gtattgatga tattcatctt tgtattgtca atatcctttc aacccaaaaa ggcacggata
    33601 ttttacgccc tgaatttggc tctgaccact ttcgctacat tgactaccct gaagatgtgg
    33661 ctatcccgaa ttttgtacgg gaaattacaa ccgccttgca aaaatgggaa aaacgcattg
    33721 aggttgaaga agtattggtg gacggttcag ctccgcattt tatttttact gtgatgtggc
    33781 acttaaccga tgatgtttat cgtgaaattt accgaacaac ggtataagga taggcaatga
    33841 cagaagcgat taaaatcatt gatgacgatg taaaaaccgt acttgccgaa gcgattgccg
    33901 attatgaaaa acgcaccggt aaaatcttac aacctgcaca tattgagcgt tccattattc
    33961 aaacctacgc ttaccgtgag cagttattac gcaaaggcat taatcacgcc tttttacaaa
    34021 cctttccgca gtttgcgacg gggattgcgt tggatttgtg cggggaaact tttggttgtt
    34081 atcggcttag agataaacct gcccgtactt tactacgctt ttcagtaagt ggtgaacata
    34141 cagctatagt tatccctaaa ggaacgcagg tggcaattaa tgacgagcta tacttttcaa
    34201 cgctcaatga cgatgttatt actccattaa ttacctatgt ggaaattgat gcagagtgta
    34261 atcaggttgg tgttatcggt aatggttggg aatcaggaag agtcagtaaa ctaaaaacag
    34321 cactggcaac taattataaa gtaacagttg aaaacattga tattacgagc ggtgggttga
    34381 tgcaggaaga agatgaacct taccgtaagc gaattttagc cgcaccggaa gcctttagta
    34441 actgtggctc tatcgctgca tatgattacc acgtccgctc tgtatcccaa gatattgcag
    34501 atgtgaatat ctctacgcct aggggtggct tggtaagaat tgcggtattg acccaaaaag
    34561 ggttaccgga tacccgatta cttgatgata ttaaaaaaca cgttagtgcc gaacggctac
    34621 gcccactttg cgatatggtc gaggttatta agcccacaga acgccgttat caaattacgg
    34681 cacgtttaat attgttagat ggctaccgtg aggatttggt aaaagcaaaa gcccgagagg
    34741 cattacaact ctatttgtca gacaagacta aaaaactcgg tttagacatt gtgccgtcgg
    34801 ccatcatttc agctttaaga gttgagggtg tttacgatgt gaatttaatt cagccagtca
    34861 aaactattat caacgaaaca gaatgggcga actgcacagc aatcagcgta gaagtcgaag
    34921 aggagcgtag agatggctag cttgcactat gcggacatta ttgtgcgaga ccctaaatat
    34981 aaagcattag cggatttaag caagcggtta aatttgctcg atacttcgca aattatgact
    35041 acgctggtgg atttattggg cgacgaatgt atcccattgc tggcagaaaa atggtcggta
    35101 accggctatg acggcttatt ggttgcagat agcgaaaact caaagcgagc cttaattaaa
    35161 agagcaatcg aactacatca actaaaaggc acgccttggt caattcgtga agtattacgc
    35221 agactaggtt tcggagaggt tgagattgac gagggactga aaaatcggct gtatgaaagc
    35281 acttatgtga gcggaatccc cgaaaacgaa cgctgggcgc actatgcgat taggttgagt
    35341 aacccgatta ccaatgagca agctcgcaat atccgtaagg tcttacgcaa ttttgctccg
    35401 gctcgttgct ctcttgcagt tttggattat aaagcagatc caattcgata taacaataaa
    35461 gcaacctaca acggcacata taaccacggt tcaagttaag gataaattat ggcaggtgta
    35521 caagaaaccg cacagtggga aaaccacgtt tacagaattg aagaaaatga cccagtccac
    35581 ggaggagaaa acggtgtaac caataagcca attaagcacc tagcaaaccg cacgctctat
    35641 ctgcgtagat tgctcaccga ggcaggacaa cgcattaacc cgaaaaaaat cacagctacc
    35701 agcaaaaata gtaatgattt aactgggcat actcacgaga ttgaccacgc aagcctaacg
    35761 caaaaaggca ttgtgcagtt aaatgcgagt atccaatcaa ctagcacaac tgagtctgca
    35821 accccttcag cagttaaagc agcctatgat aaagcagcaa cagcagaaca aaaagcgaca
    35881 ttggcaaatg agaaagtcga ggcgattaaa gtaggcggac gaaacctcct cattcaatcc
    35941 aacactccac acaacgggaa tgcgtactca gtcagatacg cattggctga ggctccgaaa
    36001 gtaggcgaag agattgtagt cactctatgg ggtgaactcg gctctcaacg tactggaatt
    36061 ggcgtttaca actctcaagg tgctacggag ttggcaaaac tggtcaaaat tcgtgatgga
    36121 gtttatcaag gtaaaggcat ttggcgtaag ccgatgagtg agggacgaga gcgaacgcct
    36181 aacgacacgc acctgaatgt gtatttttac ccgaaaaccg caacagataa taaccgtatt
    36241 gagcgaatcc agcttgagcg aggctcaatc ggtacagatt ggacgccaaa cgaggcagac
    36301 ttaaaggcag agattagtgc aatccgcact aaactaatcc ctgtacgggg tggcaatgtt
    36361 gctacaacct taaatgcggc taagcggtcg gattggttta gagaaatgca aatcccttat
    36421 caaagtggtt gggggtatta cacccaagca tttcataact cagggacgca acacaactac
    36481 agcaatttac cgctgacagg tcgttattat tttatcgccg aactctatca taactctggt
    36541 tattctcact tgcgtatcac ctatccaagt ttaaaacgca cctttgagag tgctatcaac
    36601 cttaacaatg aggatttgat tctagattgg cgtgaggtgg tgtttttaaa agatggattg
    36661 tatcaaggca cttttaaaac aacaggcaca ctagagtctg cttatcgtct aagagcggct
    36721 cgtgatgatg aacgttatca gccatacctg caaatggtcg atatgggcta tgaccttgcc
    36781 aatcccccgg taggaaaaaa caaaattatt ggcagtgttg attattacgt taaaaacggc
    36841 acaagcgagt cttccaaagc gacgatgatg gcagcggcaa tgcctgacaa gaacgtattt
    36901 tgggaagtag cattgtggaa tgcagcacag caacggaatg tattgtttaa ggggtatagc
    36961 aagactaaca atttgagtat tggtaaaacc accgacaatg agcgagaccg agtacaagtc
    37021 aatggcacaa tccaagccac cgcaccggct gataacgcca ataacgacca agtgccgact
    37081 acggcttggg tgcgtaagat ggcaaagatt ttagctgatg gcaaggtgag taaagccggt
    37141 gacacaatga ctgggcattt attcatcaag aatggggaat actctgcggt acatacctat
    37201 aacacaagtg gttggttcgc aaaatgggag gcggccccat tatctgattc gcactttgct
    37261 gccattgtat atgccaacga cagagagaca gttatcaatc gggtgttaat tcccaagaag
    37321 aatggcacag tcgctttaat taatgatatt gccgataccg ttagagccgc tgtgagtcgt
    37381 agtgataaat cccgtagtag aacttttcct ctgcaaacgg ggaaaaaata tacaaccaca
    37441 gggagcgtta cgatttatcc agatggccga attgtgcaga ttttccactt aaaaaatatt
    37501 aaagcggcgt ggtttgatta tgaggcaagt gcggtaggag ataggcatag agttgtcaat
    37561 atccctcttt ggactgcgat gccaaataaa atttttgatg tcgatgttaa aactgttcgc
    37621 ccctcaaata atgcctcaac ttactacata gaagcggctg aatggctgag tgcgtggcag
    37681 atttacggta atgacacgaa caaatcaagt gtcaatatta atctatctcg attcagaggt
    37741 ggaaaggatg aagatatgga cttgtatgta acagttgagg gatattaaat ggcatatttt
    37801 gtgaatgtga ttgatgagca aggcaattat gagctaattg atgatgagtt tattgggctg
    37861 tatccggatt tggattttaa cacattaccc aaactcacgg ataagcaata tcagacctac
    37921 cttgccaaag caaacggcaa agagcgattt gtaaatggcg aatttgtgta tgagcaaatt
    37981 aaggtggata tgcaagcggt catttccgct gaaaaatctg caaagttagc agagattaac
    38041 caaaaagcac aagcctttat caatgattta gccaaataca acgaaacgcc taagtttgag
    38101 cgtgatactt ggcttgagca agcgaaagag gcaaaagcgt gggtggctga cccaaccgta
    38161 caaacgccaa cccttgagtt aattgcgcaa atgcgaggca taccgctcga cacgctacgc
    38221 caaaaagcct acgaaaaagc gatggcgtat caaacggtgg ctgctattgt agcagggcaa
    38281 cggcaaggat acgaagaccg cctagaacaa gcggaaacct tagagcaaat acaggcgatt
    38341 gagccgatgt atcaattacc gcaaggagca aacaatgagc aaactgacta aattaaaatc
    38401 gtggagctac cacgtcttaa ttgcaataga tcaactcttt aacgctctca ccggtggagc
    38461 agcggacgaa acattttcta gccgttgcta ccgtggtgca gtgcttgcgg aaaatccgaa
    38521 aaaacgttgg cgtttttggc actctttttt gaattgctta tttttagacc caaagcactg
    38581 ctacgccgct tataaagcgg aggtacaccg caagcagtat ccgaaggcat ttagacttgg
    38641 ggggatttaa tgaaaaagca aaacttaaaa ttagtgcggg gcgatgatgt gagttttaga
    38701 ttcagcatta aagacagcac cggacaacct cttgcgttaa aggatttgtg gtttgatttg
    38761 cacttagtca gctatcaagg tgaggtgctg attgctctct ctactgatga tggcagtatt
    38821 gagacagcag aggctggtca gatggtgtta cattttagtc accagcaaac tgctcgagct
    38881 gaatggacaa gtgccaatta tgacttgcaa ctcacgcacg actatacaag agtctaggag
    38941 gtggagatga cctctatcat tgaactagac ctgactgatg acctcatcat tgaggtgcag
    39001 ttagatagca caatcattac cggcgatagc tttttagcca acctgccgga tttactccaa
    39061 atttacaacc aagcaaagtc agatagcagt aaggagctta acaatgacta aacacaaaaa
    39121 ccacgaaaac cacccgattg ctataacatt ctaccaactt ggcaaagatg tcggcgacat
    39181 taaccgccgg ctagcggtta ttgagcaacg tgagcaatca gcacagccaa gccaaccaca
    39241 agctgaggcg gttacaatgc cacagcaaga tgctgttgcc attaataaca tcaatgccgt
    39301 tttggaggcg gtcgaaaaat acaaaacttt tgaaaaatac aacactcagt ggcaagagcg
    39361 ttttgaaaaa ttcctgtctc gtaacccgaa tgattacgag ttaagtgaag agcaacgcca
    39421 aaaactttac cacacgatgt gccagcattt gtggcaggtg aactttactg aaatcaatag
    39481 taaacagtat gaattaccga tcccgcaaca attccagggt aagtttggct atattgtcaa
    39541 aacctcgggc ggtagcacag aaattagttt tgagttgcta tctgaaactc acagcactta
    39601 tctttatccg gatagctata ttcccgaggg cggtggcgat gtaatgctca agccggctta
    39661 tatttattta gtgattccgg acttagtggc aatgcgtgag ccggaggtgg tgggggtttc
    39721 accgctgaaa ctgaacattc tgcagaaaca cctgctgaca cctcagaaac tacaggagca
    39781 gaagcccttg aacacgaagg caaactgatt gatggcgtat ggcattttga gcgagagccg
    39841 gtaaatccac cgagagatca gaattattat gtggcaggta acctgttcta caacggcata
    39901 ccggattgat aaattgatgt ttcagtaaac ctaattaggg gggaatcccc cttttttatt
    39961 attataagga aagaaaatga ataaatcttt agtagcgtta gctgttgcag cgatggcatc
    40021 gaaagcacaa actaaacagc aagcaagcaa gcaagcaagc aagcaagcaa gcaagcaagc
    40081 aagcaagcaa gcaagcaagc aagcaagcaa gcaagcaagc aagcaagcaa gcattgtgag
    40141 atagagaaag tatttaaaca agctccttta cccttttcag ggcaaaaacg gatgttttta
    40201 tcccatttca aacaggtgtt aaatgcaaat attgaagcag acgggaaaga ttggactatt
    40261 atagatgttt ttggtgggag tgggttgctt gcacataccg caaagcgtga gaaaccgtta
    40321 gcaagagtga tttataatga ttttgataat tacgccgaac ggctaaacca tatcaaagag
    40381 acaaatcaac ttcgccaaga aatctaccaa attgttgatg aaattatacc aaaaaataag
    40441 agaattagca atgaaattaa agctaaaatc atcaataaaa tcaatgattt tgaggggttt
    40501 aaagacctca aatgcttaag ttcttggtta ttgtttagcg gtgagcaagt tgcaacttta
    40561 gatgagttat ttaagcacga tttttggcac tgtgtgcggc aaagtgacta tcctgaagca
    40621 acgggttatc ttgatgatat tgaggtggtt agtgagtcct ttcatcaatt attgccgaga
    40681 tttcacgaca aagagaaagt attattgatt cttgacccac cttatctttg cacaagacag
    40741 gaaagctata agcaggaaaa ttactttgat ttaattgatt tcttacggtt gatagacctt
    40801 actaaaccac cttatatctt ctttagctca accaaaagcg agtttattcg ctttattcac
    40861 tatatggttc aaaacagaaa agaaaactgg caagcgtttg agggagcgga aagaatagtg
    40921 gttaatgcct cggcaagtta taacggcaaa tatgaggata atatggtgta taagttctaa
    40981 aaatttaaac gccctttgat agtgatttaa agggcgttaa aaaaccatta aaattaagaa
    41041 agtaaagctc ctgttttctt tcgtagtttt cgtggttgcg tttcgcagtt ttgttggctc
    41101 gctacaatta ctgttcattg taatgtttaa tcgaccattg gttttgctag gatcagtttc
    41161 accaaaataa ccgttaaaac cttggacggt gccttctgct gctttgattt tttcagcaag
    41221 tatttgttgc tttgcagcac tttcatctgt taccttgagt acttcaaagt attcgttttc
    41281 acgaatgaga ccagtagcaa agtctaaatc gatgaaaact ttacgcgcat taaaaatagc
    41341 tgtgatctct gcaacgctta aaggtttttt caaacggata aaattagttg gcaagtgtgg
    41401 aaaacgttga tatgtgaaaa tatcatttgt ccaaatgacg agaaacctga tctggactag
    41461 taattgcttg aactttcact tggtcaattg ttggactaac tatgccggtt agtgtaatgt
    41521 ttgctgtttg ggctgtatta tgattgacaa ccatcgctcc ttcatcgcta ttctgaatac
    41581 ggcggaacgt gagatcattg ccgttgacat ctaagcgccc accacgataa ccaaaataga
    41641 tattgttagg atccatttga tctgatgaat tgaggattac ggtaggtctg ccactcacaa
    41701 tacctagctc tgaaaatgca gattttttgt tattttcatc cgcttgttga tttaatataa
    41761 cagtgccgtc accaacgcta acggagcctt tgttttctcc tttgccgttg atagttagtg
    41821 tcccttgtcc gatttttgat aatctgtcac cagcagggtt actgattttc cagatcacat
    41881 ttttatcttt atcaatatca acaccggctc ctaaccaagt aatgcctgct ttcatacctt
    41941 ccacagtggt attaccatga aaatggattg ctcctgcacc ttggttgata ttctctgtga
    42001 gacttagcgt attgttttca cccaaaatat ggaatgtttt accgtgttgg agttgaggat
    42061 tccaagggtt atcgcctgta ttaccattat gaccttgaag tgttggtaat gaagtatctg
    42121 ctaaattaac tgtaagagga gcattccctc ctacgattga gctggtattg ccagaaggtg
    42181 tccaagtgag tctagcacca tttgcattaa tagcgcctgc attattttct ttttctaaag
    42241 cacgtacata atgtgggtgg tgatagttcc agtaagattt ataaacatct cccccaagcg
    42301 tattattctc tgagaaatag tcactatata cacctaacac aacccatttt ttggtacgaa
    42361 catcataacc aaaaagagcg gagccactat cgccaggtaa accgagagtt gttaagggac
    42421 cgtagccgtg gtctttaaat acatcgttta tggtattatt ttcagttttt gctactaaaa
    42481 tacggctgtt aaaatcgctg cctggaacgc ttggatcgcc attagtaaca ggcagaacgc
    42541 tacctcctgt caaaaaagga ccgttaccga cttcaatacg cccgttgttt aagcgatctc
    42601 tttcgttgtt aaccactgct tgtctaccag aaccggcacg aataaacatg gggaaaataa
    42661 aagggtcgct aaatacacta taaaaatctt gtacattttc ggtagtgcgt tcaatctcat
    42721 tagctggagc gacttcagtt actaatttcg ataacctagg tgcgtggtag tcccagcggg
    42781 gcgttgggtt acctgaacca tctaaaatca gtttttctcc ttcgtcatta cgttcacgtt
    42841 tttctatgcc tttcccgaga tctgttcttt ctttatttgg atcaggcata tagttattac
    42901 ggcgcaccac actataattg taatgatggt cgtcggcttt ttcgctagat gaatcaccaa
    42961 attgaatgtc ccgatagcct tggttgtggt ttacactaat aacgtattgt ggattaacta
    43021 atgttgcaat ccctgtttga cgagtaatgg aagagaagtc aatcattgga atagacatag
    43081 tctcacctgt tactgtttca aaaacaggta gatcattaga gtcttcatca cctccaccat
    43141 agagaaattc ataacaaccg ggtaaacttt cattatcaaa gcattcagat aggcttacat
    43201 tcggcttagg tgaaatattt gattccactt caacctgctt agttggtgta acctgtaaaa
    43261 aagtaccgat gtttttgccc gttttatcaa taactggaat attggttgcc ccaacaaaaa
    43321 atttgccttt attttctgct aaatcacgaa aatattgata atcgacatca ccacgtacaa
    43381 tagatgccag taatgttggt gaaagagaaa tagcaattga tgttgctaga agagagagct
    43441 tgaaagtat
//
LOCUS       DANLYI010000002        42565 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.002, whole genome shotgun sequence.
ACCESSION   DANLYI010000002 DANLYI010000000
VERSION     DANLYI010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 42565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42565
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.002"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            <1..1599
                     /gene="pckA"
                     /locus_tag="PXD81_000058"
     CDS             <1..1599
                     /gene="pckA"
                     /locus_tag="PXD81_000058"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HDL5536134.1"
                     /translation="RIEQELADLGITNVKEIVRNPSYEQLFEEEMKSDLKGYEKGVLT
                     NLGAVAVDTGIFTGRSPKDKYIVLDETTKDTVWWTSDAAKNDNKPMTQSTWQSLKTLV
                     TDELSNKRLFIIDAFCGASPEHRINVRIITQVAWQAHFVKNMFIRPTEEELKDFKPDF
                     VVMNGSQVTNPNWKEQGLNSENFVAFNITEGVQIIGGTWYGGEMKKGMFSMMNYFLPL
                     KGVASMHCSANVGEEGDVAVFFGLSGTGKTTLSTDPKRKLIGDDEHGWDENGVFNYEG
                     GCYAKTINLSIENEPDIYRAIRRDALLENVVVREDGTIDFDDKSKTENTRVSYPIYHI
                     DNIAVPSKAGHAKKVIFLTADAFGVLPPVSKLTPEQTKYYFLSGFTAKLAGTERGITE
                     PTPTFSACFGAAFLSLHPTQYAEVLVKRMEAAGAEAYLVNTGWNGTGKRISIKDTRGI
                     IDAILDGSIEKAEMSTLPIFNLAIPTALPGVETHILDPRNTYEDKAQWQAKAEDLAGR
                     FVKNFEKYATNEEGKALIAAGPKL"
     gene            1692..2693
                     /gene="bioB"
                     /locus_tag="PXD81_000059"
     CDS             1692..2693
                     /gene="bioB"
                     /locus_tag="PXD81_000059"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HDL5536135.1"
                     /translation="MTTFQLRLKAKDELTPHPTAQYWRKCQVEGLFDLPFMDLVFRAA
                     QVHRENFDANAIQLSTLLSIKTGGCPEDCEYCPQSARYDTGIEKQTMLDVETIVEKAK
                     IAKERGASRFCMGAAWRGPRPKDIKVVSEIINAVKSLGLETCGTFGMLEEGMAEELKQ
                     AGLDYYNHNLDTDPERYNKIIHTRNHEDRMDTLGKVRNAGLKVCCGGIVGMNETRPER
                     AGLIASLANLDPQPESVPINQLIKVEGTPLTNAEDLDWTEFVRTIAVARITMPQSFVR
                     LSAGRTTMPEAMQALCFMAGANSIFYGDKLLTTENPEEDHDRQLMDKLDLYPLQYIA"
     gene            2853..2950
                     /gene="ffs"
                     /locus_tag="PXD81_000060"
     ncRNA           2853..2950
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PXD81_000060"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            2985..3692
                     /gene="gloB"
                     /locus_tag="PXD81_000061"
     CDS             2985..3692
                     /gene="gloB"
                     /locus_tag="PXD81_000061"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HDL5536136.1"
                     /translation="MLQITPIPALKDNYIWAIRNGQDVIIIDPSEHQPALNFIAKNAL
                     NLTAILITHNHHDHTDGVSGIVEQYPNIPVYGPQEVAEFANVIVSPEQHISLFGYDVR
                     IIESGGHTAGHISYLFGYDYLFCGDALFSGGCGRVFTGDYQAQFDALQRFKNLPNSVK
                     AYPAHEYTKSNLKFAEAVLPPSCVLSEYQEQVDLLRSQNKPTLPTTIGLEMQVNPFMQ
                     AVDLAEFIALRQQKDNF"
     gene            3851..4564
                     /gene="uppS"
                     /locus_tag="PXD81_000062"
     CDS             3851..4564
                     /gene="uppS"
                     /locus_tag="PXD81_000062"
                     /EC_number="2.5.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005619022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="di-trans,poly-cis-decaprenylcistransferase"
                     /protein_id="HDL5536137.1"
                     /translation="MNFDPQNMPKHVAIIMDGNGRWAKQKGKLRIFGHQNGVKAVRSA
                     VSFAAKHHIQVLTLYAFSSENWSRPETEVSALMSLFMKALDSEVKQLHKNNIRLKIIG
                     DKSAFSENLQKRIAESEKLTENNTGLILNIAANYGGYWDIVQAAQQLAVHVKSEQLAV
                     EQITPALFQQHLVTENQPQVDLLIRTSGEQRISNFLLWQIAYAELFFSPVLWPDFNDE
                     AFSDAIYAYQQRERRFGGC"
     gene            4588..5457
                     /locus_tag="PXD81_000063"
     CDS             4588..5457
                     /locus_tag="PXD81_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005622689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HDL5536138.1"
                     /translation="MLKERVLSAIIMIIAVLAAIFWLSPLPLTLVLSAIVVAAMWEWA
                     QFAGFKHPVPRAIVAMVTICLLLFAIVANTDYIRATRFITDETTPILFIGCIWWLIAL
                     VLVATYPNSAKLWEKSVAAKFLFGLATLVPFLIGTLALRFHNYAFDPYQGTYLFLYVC
                     LLVWGADSGAYFAGRAFGKRKLAPKVSPGKSWEGVIGGVLTSSLIAFTFLQLTPANVF
                     GKALATTPFILVSVGMVAISVLGDLAESMFKRQAGIKDSSQLIPGHGGILDRIDSLTA
                     AVPFFATFFFFVL"
     gene            5461..6771
                     /gene="rseP"
                     /locus_tag="PXD81_000064"
     CDS             5461..6771
                     /gene="rseP"
                     /locus_tag="PXD81_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005603714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HDL5536139.1"
                     /translation="MTSIIAFFILICVLVFVHEYGHFWAARKCGVKVIRFSIGFGKVL
                     FKKKDKQGTEFVFSLIPLGGYVQMWNDETDINAPAQQALNTKSVLQRAFIIIAGPAAN
                     FIFAILAYWVVFIAGIPTVKPVIGEILPNTIASQARIPTELEITKIGHQSIQDWESAS
                     LALVGFVGNKNVPLEGNINGNSTQKYELDLSNWSIDSAKENPLTALGIRPKSAKVTAE
                     IKQVVENSPASQVGIQAGDRILQINQQPFNWFNLVELVQAGKPIELKIEQRGQIKDLV
                     VQPEKKDERYIIGVIPSYEPLADKYRTELKYDILTAFYKSIEKVWSLTQTILQFIGNL
                     ISGDLSIKNLGGPISMAKGAGATAEIGLVYYLSFMALISVNLGVMNLFPLLPLDGGQL
                     VLLAVEAIRGKALSEKIQLKFQQIGFAFVLSLMLFAFANDIIHF"
     gene            6886..9267
                     /gene="bamA"
                     /locus_tag="PXD81_000065"
     CDS             6886..9267
                     /gene="bamA"
                     /locus_tag="PXD81_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005600511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HDL5536140.1"
                     /translation="MKKLLLSSLLIANGVVAAPFVVKDIRVEGVQPTTGESIISSIPV
                     RVGQTATDTDVSNVVRHLFSQQRFADVRATREGNTLVIKVAEKPIIGKVEIEGNQAIP
                     KDALEQNLKANLINQGEIFDAAKLEAFKEGLIEHYHSTGRYEAKIDTSVSNNTEGGVN
                     IKLAINEGEVAKAKTIKFEGNNAFSDKKLLENLDIQPDVSWWNIFESSKFEQTAYQKD
                     LETLRDFYLNRGYAKFAIQDTDVQFNDNKTEVALTYKLDEGSQYHVSEMRIIGNTANM
                     DSQLNALLKEFKPGQLFRKSDLTQIEEEIKSILGDAGYGSAKVDIYPQFNEENKTVKI
                     SFVVDAGRRIYVRKIRFEGNGVTADSTLRREMRQQEGAWLSTNKASLGKARLERTGFY
                     ESVDMSMPNVEGTADQVDVVYKIKERNTGSINFGIGYGTKNGISYQAGIKQDNFLGMG
                     STISLNGTRNNYGTSVNLGYTEPYFTKDGVSLGGNIFYEDYDNSDNDSMASYKRQTYG
                     INGTLGFPVNENNSYYLGLGYTHDKIKNAAREYTREKYVKSMNFEFDPAADYYKKIKA
                     DDFDFSLGWNYNSLNRGYFPTEGTTANIGGKITIPGSDNKYYRVSADFRNYYPLNREH
                     KWVISTKASIAYTNGFGGKEVPFYQLYTAGGIGTLRGFSYGAVGPQAIYYNTKSGKFD
                     SMNNDIIGGNALAAASLELITPTPFVSEKYQHNVRTSLFVDAASAWNTKWKKDEYTNL
                     PDYGDFKRFRASAGIAFQWMSPIGPLSFSYAKPIRKYDNDDIEQFQFNIGSSF"
     gene            9347..10138
                     /locus_tag="PXD81_000066"
     CDS             9347..10138
                     /locus_tag="PXD81_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005614636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HDL5536141.1"
                     /translation="MKKLFKMTGLAVALASTTGIANAADTIGFVDPSYVLQNHPVLLD
                     ASAKFDKFMKESQEKFAADEKKLADENKTLTAERNKINEDAQKLQNEQKNVEASIKKK
                     VAALEKDAPRLRSKEIQARQNAIQKEANAFQAKVTAIQKREADFAKKAEAFQKRADEF
                     QKRIEQANKENGGINPQEAQKQAVDEVNATIKEIAKSKGYTLVLQPQVALYAEDEGKD
                     ITEAVLDAIVKKHPEIKIEKPATEAQPATEKETKAEETKPEEAKK"
     gene            10138..11163
                     /gene="lpxD"
                     /locus_tag="PXD81_000067"
     CDS             10138..11163
                     /gene="lpxD"
                     /locus_tag="PXD81_000067"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005603709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HDL5536142.1"
                     /translation="MANFRLIDLAEHIGGTLRGNADLAIQSIAALDKADSSQITFISN
                     AKYRENLTACNAGAIIVSPADVEFCRADQNLIIVANPYVAYAQLAQYMDSTPKAASEI
                     HPSAVISPEAKLGNNVSVGANAVIESGVELGDDAVIGAGCFVGKNSKIGARTQLWANV
                     SVYHNVQIGADCLIQSSTVIGSDGFGYANDKGQWIKIPQTGGVIIGNRVEIGACTCID
                     RGALDPTVIEDNVIIDNLCQIAHNVHIGFGTAVAGGVIMAGSLKVGRFCQIGGASVIN
                     GHMEICDGAIITGMGMVMRPITEKGIYSSGIPLQTNKEWRKTAALVMNIDEMNKRLKS
                     LEKRFND"
     gene            11192..11650
                     /gene="fabZ"
                     /locus_tag="PXD81_000068"
     CDS             11192..11650
                     /gene="fabZ"
                     /locus_tag="PXD81_000068"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HDL5536143.1"
                     /translation="MTTENREPNIIEVTEIMDMLPHRYPFLLVDRVTDYEEGKWLKAI
                     KNVTFNEPCFTGHFPGAPIFPGVLILEAMAQATGVLAVATYGKLSEDELYYFAAIDNA
                     RFKRPVVPGDQLQLEVEFLKEVRGITKFTGKAYVDGKLVCEADLMCARRK"
     gene            11684..12478
                     /gene="lpxA"
                     /locus_tag="PXD81_000069"
     CDS             11684..12478
                     /gene="lpxA"
                     /locus_tag="PXD81_000069"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HDL5536144.1"
                     /translation="MRLIDSTAKISPLAIIEEGAEIGAHVEIGPFSVIGKDVKIGART
                     KIHSNVVINGMTEIGEDNHIFQFASIGEINQDLKYQGEPTKVVIGNRNRIRESVTIHR
                     GTVQGGGVTKIGDDNLFMINTHIAHDCSIGNRCIIANNGTLAGHVTLDDFVIVGGMSA
                     IHQFVVIGSHVMLGGGSMVSQDVPPYVMAQGNHARPFGINFEGLKRRGFDKPTMHAIR
                     KVYKLIYSSGKTLEECLVEIEQIAATEPAIAIFKQFFKRSTRGIIR"
     gene            complement(12516..14516)
                     /gene="mnmC"
                     /locus_tag="PXD81_000070"
     CDS             complement(12516..14516)
                     /gene="mnmC"
                     /locus_tag="PXD81_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HDL5536145.1"
                     /translation="MGKLTFATLTFNDADTPVSEQFDDIYFSTQDGLAESYYVFQEGN
                     QLWEKWQSHPKEAFVIAETGFGTGLNFLAVADKFQQFRAAFPQSKLKRLYFISFEKFP
                     LTASQLADIHQRYPQFATLSENLTACWQPRQIGCQRYHFEQVYLDLWFGEIAENLPQL
                     GDLYSGTIDAWFLDGFSPDKNPEMWNEQLYLQMFRLSANGGSFATFTASSMVRRGLEA
                     AGFAVKKRKGFGKKREMLWGEKPLDSQVSEPNYPYFYAKNETADDIAIIGGGVASFFV
                     ALSLLEKGKQVTLYCKDETLAINASGNLQGAIYPQLSDDDERNVQFYIHSFDYALQRL
                     QQLKSNIEFEHNLSGVVLYAYDEKTAKKLEKMVEQTADDTLLKSCSQAELSEKLGLSV
                     PNSGAFIPQAGWLSPVQLVRNGFTWLEQKGLKIVLNHKVEHLTFSNGMWQWQTRGETV
                     RHQAVVLANGHTLNEFSQANGIPLYPVRGQVSQIPTTSELQKLKCVLCYDGYLTPMSA
                     YGTHCIGASHIRDNAETEFSLSEHWENVAKLQQNVTVCEWTQGIDLSENKAKVGIRAA
                     FRDRVPMVGAVHHFEQQKSQYANLYNQLRRKQAVENAEIFTNLYMVNGLSSRGLTTAP
                     LLGELLASLIVNEPVPISEDIWHNLSPNRAWIRKLLKGSTVA"
     gene            complement(14741..16177)
                     /gene="trpCF"
                     /locus_tag="PXD81_000071"
     CDS             complement(14741..16177)
                     /gene="trpCF"
                     /locus_tag="PXD81_000071"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HDL5536146.1"
                     /translation="MQNIVQDNAQPNQPTILQKIVQDKAIWVAQKEQAFPLSEFQHKI
                     VKSDHDFYAALAKGTHQEPAYILECKKASPSKGLIRAEFDLDSIAQVYKNYAAAISVL
                     TDEQYFQGDFRYIDQVKRQTTQPILCKDFMISAYQVYLARFYNADAILLMLSVVNDET
                     YRELATLAHELGMGVLTETSTEAEFERALALGAKVIGVNNRDLHTLTIDMNRIVRLVE
                     KYQAQISADVCLISESGINDHQQVKNIKPYVHAFLIGSSLMGSADLNNAVRAVIFGEN
                     KVCGLTRVQDVKAVYEQGALYGGLIFAEGSPRQLSLRQAQELVVNAPLRYVGVFQNQA
                     VEFVEKMAKQLELFAVQLHGSEDEQYIAELAEKLGSKTQIWKALSVDVEVENLEFNDN
                     PQIARYVLDSKVGNQQGGTGKTFNWALIPETLKQKALLAGGIGTENIEQALAQGCVGL
                     DLNSGVESAKGVKDLQKLTACFGKIIQA"
     gene            16265..16576
                     /locus_tag="PXD81_000072"
     CDS             16265..16576
                     /locus_tag="PXD81_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005623108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HDL5536147.1"
                     /translation="MKSQVIQHLSYLEIALRVNQLWEATPPSPEALANEQPFCVETLT
                     PTQWLQWIFIPRMRAILDANADLPRNFAITPYLEEALKNEGYLQAICEPLLGLEKLLK
                     D"
     gene            16730..17458
                     /gene="truC"
                     /locus_tag="PXD81_000073"
     CDS             16730..17458
                     /gene="truC"
                     /locus_tag="PXD81_000073"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009874610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HDL5536148.1"
                     /translation="MTLDILYQDNSLIAINKPAGVLVHRSWLDKHETVFAMQTLRDQI
                     GQHVFPIHRLDRPTSGVLLFALNSETARIMSEQFEQHLVQKTYLAVVRGYLQGSAQID
                     YPLKVILDKIADKFSQEKEAQAAITDYKGLATVEMPYPVGKHQTSRYSLVELSPKTGR
                     KHQLRRHLKHLFHPIMGDSKYGDLHQNRALVEKSGVSRLMLHAHKLQFQHPKNLQKIE
                     IIASLDEQWQRLFAFFDWNFTQYY"
     gene            17476..17640
                     /locus_tag="PXD81_000074"
     CDS             17476..17640
                     /locus_tag="PXD81_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HDL5536149.1"
                     /translation="MDNSLLSLTHEQQQAAVEEIQKLMAQGISAGEAIQIIANQLRDK
                     YQILEKSNNE"
     gene            17633..18109
                     /locus_tag="PXD81_000075"
     CDS             17633..18109
                     /locus_tag="PXD81_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5536150.1"
                     /translation="MNKLWTVLALTITSHYSFAAAEKIVTKDLPAEGKPAVVLQVFDE
                     SEKIAKPIKGNKLFISKKQTRLCWHSINLPVQNKVTIAEAFYAPAAMKLASPGSKINS
                     SADKKNHTIITEINLANNQVLNRCWGFDKTDPVGKYKMEIQINDHIFKGLEFEIVK"
     gene            complement(18146..18997)
                     /locus_tag="PXD81_000076"
     CDS             complement(18146..18997)
                     /locus_tag="PXD81_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HDL5536151.1"
                     /translation="MENQKQAEIELKIMLEQQNVALVENYLNQTSPFPVLAHQTELLG
                     NTYYDTPEQFFATQKMGLRVRSVNQQYEITLKTQGEIVGGLHIRPEYNLALPNSQPDF
                     QTLVEKYQLPFENSEQIAESLQAIFSTDFTRKTWLVQVGMSDIEVALDQGIIKNHFGE
                     EPICELEFEIKQGNLGDLFTLVEAMPKADGMWLSNLSKAQRGYLLGQAVKWEQKIATA
                     MDGENSEKLTQLLADFICVGNESLEVLVRFNQCTQQQFSNWQQARAFVKSKDYLLHNL
                     TNLKTMI"
     gene            19058..20467
                     /locus_tag="PXD81_000077"
     CDS             19058..20467
                     /locus_tag="PXD81_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HDL5536152.1"
                     /translation="MFSKIHHKLNNFVQRGLDNHLRLAVTGLSRSGKTAFITSLVDQL
                     LHINKGGNSHLNLFAPARHGQILSVKRVEQGDLTIPRFEYDKNRQSLENQPPQWPEST
                     TGISEIRLAIRYQRQHSLLRHIKETGTLYLDIFDYPGEWLLDLPLLSLSYKEWSQTQQ
                     TIHKDERAELATPWLQAVKKLDLFAKTDENLLAEFSQQYTDYLLACKAQGMQYIQPGR
                     FVLPTENAKGAPVFQFFPLLDLSEAEWEKLEQSDRNSIFHTLKKRYEHYQKKIVKPFY
                     EDYFSQFDRQVILADCLTPLNYGYNEFMEMKLGLQQLFKHFHYGNRSLFHRLFSSNID
                     KLLFIATKADHITSDQVPNLESLMRQLVQEGGRHAEFDGIETGYLAISSIQATDAVKV
                     TQQDKIIKAIRGIRSSDRKQVTLYPGSVPNRIPDKNYWQFNRFEFDQFEPKQLDYSQA
                     IPHFRMDSVLQFLLADMFE"
     gene            20513..20863
                     /locus_tag="PXD81_000078"
     CDS             20513..20863
                     /locus_tag="PXD81_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDL5536153.1"
                     /translation="MNTVISQRKPIHWVGSTLDDIREFPDNIKRELGFDLDLVQQGLL
                     PRDFKAMANLGSGVMEIRVKDPSGAFRLVYLAKFKDAIYCLHAFQKKTQKTAPKDIAV
                     IKARYAAVKELENE"
     gene            20856..21179
                     /locus_tag="PXD81_000079"
     CDS             20856..21179
                     /locus_tag="PXD81_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HDL5536154.1"
                     /translation="MNNIDTKIRHTTEAQGNVFEDLGFPIDEAQKLKSASQQLIETKL
                     MLMNEMSNWIDKNNLKQSEAASILGVSRPRISDMVNGKLEKFTLDTLVTFVAKTGKTI
                     QLQLS"
     gene            21287..21766
                     /gene="smpB"
                     /locus_tag="PXD81_000080"
     CDS             21287..21766
                     /gene="smpB"
                     /locus_tag="PXD81_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HDL5536155.1"
                     /translation="MAKKPKVASNTIALNKRARHEYFIEDEIEAGLELQGWEVKALRA
                     GKGNISDSYVIFRHGEAFLFGAMITPLNMASTHIVSDPTRTRKLLLNKRELDSLFGKV
                     NREGYTIVATSMYWKNAWVKVKIGLAKGKKLHDKREDIKDREWQVAKQRIMKNANRG"
     gene            21969..23276
                     /gene="serS"
                     /locus_tag="PXD81_000081"
     CDS             21969..23276
                     /gene="serS"
                     /locus_tag="PXD81_000081"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009874613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HDL5536156.1"
                     /translation="MIDQNLLRTNLEDVATALKIKRNFILDVERVKTLEEQRKTLQVK
                     TETLQAERNTRSKNIGAAKARGEDISALLAEVDSMGNELDTAKVELDKVQAEIRELLL
                     SVPNLPADEVPLGKDDSENLEVLRWGEPRQFDFEVQDHVTLGENLNGLDFAAGVKLTA
                     SRFVVMKGKLARLHRALSQFMLDLHTEQHGYVETNVPFLVNHDTLYGTGQLPKFGEDL
                     FHTQPLTGQDPNEVQRPFGLIPTAEVPVTNLVRDEIIDEDSLPLRYTAHTPCFRAEAG
                     SYGRDTRGLIRMHQFEKVEMVQIVAPEKSMEALEELTGHAEKVLQLLGLPYRKVLLCT
                     GDMGFGAAKTYDLEVWLPAQNTYREISSCSNMWDFQARRMSARCKAKGDKKTRLVHTL
                     NGSGLAVGRTLVAVLENYQNADGSITVPEVLRPYMGGLEVITA"
     gene            complement(23335..23661)
                     /locus_tag="PXD81_000082"
     CDS             complement(23335..23661)
                     /locus_tag="PXD81_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5536157.1"
                     /translation="MVSVDFKNNLLEFIFYGLFSEHTQTNIINGLQFYPKAGVAFRKD
                     LRFRKRAVYQREIMVFYQLDKIDEPKKVTIIAFRLGRMKNPIRLDTVQNRRVVTEIFM
                     NYPEKD"
     gene            complement(23654..23866)
                     /locus_tag="PXD81_000083"
     CDS             complement(23654..23866)
                     /locus_tag="PXD81_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5536158.1"
                     /translation="MSIVIVDTDFVVHKEDEEWTTEEIKMIEEHLLEEQSAQYQVKRE
                     VPKQFYADIAERLSRGDLTAWEQLDG"
     gene            24053..26638
                     /gene="leuS"
                     /locus_tag="PXD81_000084"
     CDS             24053..26638
                     /gene="leuS"
                     /locus_tag="PXD81_000084"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005601197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HDL5536159.1"
                     /translation="MQQQYNPSEIEPKVQRYWAENKVFKAVKDVTKEKYYCLSMLPYP
                     SGKLHMGHVRNYTIGDVVSRYQRMIGKNVLQPMGWDAFGLPAEGAAVKNNTAPAKWTY
                     ENIEYMKGQLKMLGFSYDWDREVTTCRPEYYKWEQWFFTELYKKGLVYKKTSTVNWCP
                     NDETVLANEQVHEGCCWRCDTPVEQREIPQWFIKITDYAEELLNQLDNLPQWPDQVKT
                     MQRNWIGRSEGVEITFKIAGSNVELPVYTTRPDTFFGVSYVAIAAAHPLAELAAQNNP
                     ALAEFIREAKNTKVAEAELATMEKKGMATGLFAIHPLTGKEVPVWVANFVLMHYGTGA
                     VMAVPAHDERDFEFAQKYDLQINQVIQPLDGSEWDFSKAAYTEHGKLINSAEFDGLEF
                     DVAFTAIADKLEAMGVGKRQVNFRLRDWGVSRQRYWGAPIPMMTTEEGEVVTVPLQDL
                     PVILPEDVVMNGVQSPIKADPEWAKTTYNGKPALRETDTFDTFMESSWYYARYTCPQY
                     HEGMLDSDEANYWLPVDQYIGGIEHATMHLLYFRFFHKLLRDAGILNSDEPATKLLCQ
                     GMVLADAFYYTSPTNERIWVSPTQVTLERDEKGRIIKATDPQGRELVHSGMTKMSKSK
                     NNGIDPQEMVEKYGADTVRLFMMFASPAEMTLEWQESGVEGAKRFLGRVWNLVYSYAQ
                     NPATTALDVNALDKDQKELRRDVHKTIAKVSDDIGRRQTFNTAIAAVMELMNKLTKAP
                     LETEQDKAVMAEALSAVVRMLYPITPHICFELWQVLGNEQTIDFAPWVQADESAMVED
                     EKLVVVQVNGKVRGKITVSATATEDEVKAIAKADPNVAKFLDGQQIVKEIYVPFKMLS
                     FAVKA"
     gene            26838..27326
                     /locus_tag="PXD81_000085"
     CDS             26838..27326
                     /locus_tag="PXD81_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5536160.1"
                     /translation="MIKKLSLIAVFALTACGWHFKNNEVLPESFRTLTFESADQHSEM
                     SRILRNQLQLSDVKLVPSTANVAKLRLVSTSTDSKVVSVFKQAREAEKILTLNVDAIA
                     DIPNKGQYPLEVSVHRTFFDDSRAALAKSAEKEMIMKDMYEQASRQLIIKMAGLKKEL
                     SK"
     gene            27369..28394
                     /gene="holA"
                     /locus_tag="PXD81_000086"
     CDS             27369..28394
                     /gene="holA"
                     /locus_tag="PXD81_000086"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005623136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HDL5536161.1"
                     /translation="MQKIFPETLQFSLNKGLQPFYLLTGNDLLLVNETKDAIIHTARL
                     NGFDEKKEVEIKNDTNWEELFEAAQSMGLFFARQIMVLNFPDSPTATQFKKLAELCAF
                     IHSDLLFILHLPKFSKTMEKQAWFTQIELNLVQINCQTPELSKLPQWVSHRAKAMNLQ
                     IENEANQLLCYSYEGNLLALKQALQLLQLNATDGKITLTKAQEVVEHSAQFTPFQWID
                     ALFEGKIQRAQRILYHLQNEEVQAVVLLRIVQKELMILLEITRNPTAVVSSEQSLFIG
                     NLRTEFDRLKIWQNRRPFYNQITQRLTYQKLYALIQQLAELERKVKQEFSDDIWQELE
                     RFSAQFT"
     gene            complement(28395..28580)
                     /gene="yacG"
                     /locus_tag="PXD81_000087"
     CDS             complement(28395..28580)
                     /gene="yacG"
                     /locus_tag="PXD81_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HDL5536162.1"
                     /translation="MPETIVNCPTCEKEVVWSKESKYRPFCSERCQIIDLGDWAAEKH
                     SIASEEAELFSEDLEKY"
     gene            complement(28562..29197)
                     /gene="coaE"
                     /locus_tag="PXD81_000088"
     CDS             complement(28562..29197)
                     /gene="coaE"
                     /locus_tag="PXD81_000088"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HDL5536163.1"
                     /translation="MTYVVGLTGGIGSGKTTVSDLFSALGVEVIDADVVARQVVEPGT
                     PLLAKIVEHFGEEILTAEKALERTKLRQIVFNNEEEKTWLNNLLHPAIREEMVKKLQE
                     STACYVIWVVPLLIENKLTEYCDRILVVDVSPEIQLERATKRDNSKIETIKNIMAAQV
                     SREERLSYADDVIENNLPFEQGLLLIKEQVQALHYKYLALAKKEKECQKLL"
     gene            complement(29233..29889)
                     /locus_tag="PXD81_000089"
     CDS             complement(29233..29889)
                     /locus_tag="PXD81_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HDL5536164.1"
                     /translation="MLILFSLFIAVWLKAEIPRFAIRVNRQIYQETTSLVNIGLTEQQ
                     FLAQSVLQPKQPRSANLFFLLFPLIMLCSESPIIGTIFILLCFLSLLDMCYYLTDIRY
                     LVLIFLLVLWESLADFQHQTFTFTLAFCLLIGLVSQLMFKKEAFGSGDMLLLVALSPL
                     FRLEKMLLLILTASLLGIGYYLAYWLMKKQKLAKLAFIPFISTAMLLVLMLESRGINY
                     "
     gene            complement(29892..31088)
                     /locus_tag="PXD81_000090"
     CDS             complement(29892..31088)
                     /locus_tag="PXD81_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HDL5536165.1"
                     /translation="MTKIYEFHWQAQSRFGQKQRGKRLATNRDMLENLLLAQGYQHIR
                     ISRNFVLSANPKKEEVNSLLSQLALLVNSAMPLKQALSMLLQNCRNIRLYLWLNELIQ
                     HIESGYAFSQSLEKLGEFVTNQEIQLIKMGEQSGKLGIMLTNLAESRAKLEKLAKKVK
                     KILFYPVIVLLISLALSIGLLVLIVPQFAQLYGSKDKTLPLITDILFTLSECLIEQGT
                     VLLGTLLLAVIFYLFFAKKSRLVAKWKMALWAKMPLFSRIIEQSRIIFFSQNIALMLN
                     AHIPLDVALRSFLSEKNDDPILQQEIGTMLNLLQQGYKFSQGINPNVFGAEMAQMLEV
                     GEKSGNVAKMCEHISEMYQQKLDYQIDILSQLLEPMLMLIMGVIVGTIIVGLYLPIFD
                     MGALVE"
     gene            complement(31085..31537)
                     /locus_tag="PXD81_000091"
     CDS             complement(31085..31537)
                     /locus_tag="PXD81_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilB"
                     /protein_id="HDL5536166.1"
                     /translation="MEYSVTDLNSQRFIEISAEQWQKNCNEKQILLRYLAIPLQETEE
                     QLWLGIDDMQNLNACEIFAFIYHKQIEPVLISSDELKFLLNALSPEQSSRQSEIYEEN
                     TAYSLAHSEQVDRNDPIIQILENLFKFCLQHNASDIHFEPQKEKLLRQ"
     gene            complement(31558..32037)
                     /gene="ppdD"
                     /locus_tag="PXD81_000092"
     CDS             complement(31558..32037)
                     /gene="ppdD"
                     /locus_tag="PXD81_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HDL5536167.1"
                     /translation="MQKIVHFRPLGIRQLHRAFTLIELMIVIAIIAILATIAIPSYNS
                     YTQKAALSELLQASASYKSDVEVCIYNSGGLDNCSGGTNGIQANKTSSNTTKYLNSIA
                     VTKGVITVTGKGSLNGYGYTLTPSFSNNTISWTASCSGNDTSLFPANFCSTTVTPNG"
     gene            32218..33597
                     /gene="radA"
                     /locus_tag="PXD81_000093"
     CDS             32218..33597
                     /gene="radA"
                     /locus_tag="PXD81_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HDL5536168.1"
                     /translation="MAKAPKTAYVCNDCGAEYSRWMGQCKECKAWNTISEVRLISSKE
                     AAKSDRFSGYAGETSGKVQTLSDISLQEVPRFSSGFNELDRVLGGGVVPGSAILIGGH
                     PGAGKSTLLLQVMCGLAQHLPTLYVTGEESLQQVAMRANHLGLPTANLKMLSETSVEH
                     ICNIADQEKPKIMVIDSIQVMHLSDIQSSPGSVSQVRECASFLTRYAKTRQVAIIMVG
                     HVTKDGTLAGPKVLEHAIDASLLLEGESDSRFRTLRSQKNRFGAVNELGVFAMTEQGL
                     KEVKNPSAIFLSRSEEQTPGSSVMVLWEGTRPLLVEIQALVDHSMLANPRRVAVGLDH
                     NRLSLLLAVLHRHGGLQMSDQDVFVNVVGGVKVTETSADLALILALISSFRNRPLPQD
                     LVIFGEVGLAGEIRPVTSGQERISEAAKHGFKRAIIPHGNVPKKAIKDMQVFTVKKLS
                     DALDILYNL"
     gene            complement(33652..33882)
                     /locus_tag="PXD81_000094"
     CDS             complement(33652..33882)
                     /locus_tag="PXD81_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HDL5536169.1"
                     /translation="MDYVEILGYVAMILVACSFLLKDVIKLRVVNSLGCACFVVYGLL
                     IGSIPVTGLNALVVCINLYYISKGNRPTAAHS"
     gene            complement(34002..34496)
                     /gene="folA"
                     /locus_tag="PXD81_000095"
     CDS             complement(34002..34496)
                     /gene="folA"
                     /locus_tag="PXD81_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HDL5536170.1"
                     /translation="MKISIIVARTINKVIGKDNQMPWHLPVDLAWFRQNTLGKPVIMG
                     RKTYESIGRLLPKRPNIILSRSGFTVEGAYAVQSLEQAVELAKNFANTAEIMIIGGGE
                     LFKQALPLAERLYLTEIQADIEGDTFFEFDEQNWALVEEKWSEIDENNPFRCRFMCLE
                     RKSV"
     gene            34572..34727
                     /locus_tag="PXD81_000096"
     CDS             34572..34727
                     /locus_tag="PXD81_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl kinase"
                     /protein_id="HDL5536171.1"
                     /translation="MATLFLVTSEQIMSKTIVIKFGTSTLTHGTKSLSRPYMLELVKL
                     NYTNSIV"
     gene            34724..35692
                     /gene="proB"
                     /locus_tag="PXD81_000097"
     CDS             34724..35692
                     /gene="proB"
                     /locus_tag="PXD81_000097"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HDL5536172.1"
                     /translation="MIVVTSGAAAAGRDYLGHPELPKTLASKQMLAAVGQSQLIRVWE
                     NLFDIYNIHIGQMLLTRADLDDRERFLNARDTLDALLAQKIIPVINENDAVATEEFKV
                     GDNDNLSALVAILAQADQLYLLTDQEGLFSADPRSNPDAKLLSVVEKITPEIRQIAGG
                     SSTGLGTGGMSTKITAADIATRSGVETIIASGSRPNVLVDLANGQNIGSKFLVQSDRL
                     EGRKRWLFAAPQVGEIVVDFGAEQALLQHKSLLPVGVSAIKGEFTRGEVIKIFSQDGK
                     ALALGIARYSSEALLRIKGKKSKEIESLLGYEFGSVAVHSDEMVVY"
     gene            35692..36456
                     /locus_tag="PXD81_000098"
     CDS             35692..36456
                     /locus_tag="PXD81_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HDL5536173.1"
                     /translation="MELGIDVLAILFAVATLAGFIDAIAGGGGLLTIPALMAAGLPPA
                     MALGTNKLQACGGSLSSSLYFIRKKAVNLKQIWLLILCTFLGSAAGTIFVQMINVDFL
                     KTLLPFLILIIGIYFLVSPKIGDEDRKQRISFTLFAFTAAVALGFYDGMFGPATGSFM
                     TLAFILLLGFNLSKAVAHAKVINFTSNFASLIFFTLGGAVMWKIGLIMMVGQFIGGTL
                     GAKMVMTKGKKLIRPMLVTMSFMMVGKMLYEQGIFN"
     gene            36459..37091
                     /gene="nth"
                     /locus_tag="PXD81_000099"
     CDS             36459..37091
                     /gene="nth"
                     /locus_tag="PXD81_000099"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005721589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HDL5536174.1"
                     /translation="MNKAKRIEILTRLRNENPKPTTELNYSNPFELLIAVILSAQATD
                     KGVNKATDKLFPVANTPQAILDLGVDGLKEYIKTIGLFNSKAENIIKTCRDLIEKHNG
                     EVPEDRDALEALAGVGRKTANVVMNTAFGHPTIAVDTHIFRVSNRTNFAPGKNVVQVE
                     EKLLKVVPDEFKVDVHHWLILHGRYTCIARKPRCGSCIIEDLCEFKEKTE"
     gene            37288..38538
                     /locus_tag="PXD81_000100"
     CDS             37288..38538
                     /locus_tag="PXD81_000100"
                     /EC_number="2.7.7.72"
                     /EC_number="3.1.3.-"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional CCA addition/repair protein"
                     /protein_id="HDL5536175.1"
                     /translation="MDIYLVGGAVRDQLLGLPVKDRDFLVVGATAEQLLAQGFQQVGA
                     DFPVFLHPETKEEYALARQERKNGNGYNGFICDFSPNVTLEQDLIRRDLTINAIAQDE
                     NSTLFDPYGGVQDLENRLLRHVSPAFSEDPLRVLRVARFAARFHSFGFTIAPETLKLM
                     REMVQSGELKHLTAERVWLETQKAFETDNPHVYFDILRLIGALKVLFPELDRLFGVPQ
                     PPQHHPEIDSGKHTLMVLQQAKRLAKKAENPTALLFAALCHDLGKGLTPADILPHHYG
                     HEVKGIQPTRELANRLKIPTEIKDFALLVTEYHTHCHKMAELRPETVIKLFNSLDVWR
                     KPNRFFDFLFACEADARGRLGFEQRNYPQPELAKKYYQAAIEIDVQQVIQDGFEKQAI
                     REELNKRRVYAVRSIKLDSDLINH"
     gene            complement(38639..39574)
                     /locus_tag="PXD81_000101"
     CDS             complement(38639..39574)
                     /locus_tag="PXD81_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HDL5536176.1"
                     /translation="MSESKPFPITTGFFAMPLGLGALGLAWLNATPITPYATTIGNII
                     AGTSVILWVALLGCYVYRVFFCFEQIKAEFMHPVQCCLFSLVTMTTMISGDVLYVWDF
                     YLGELLIFLGVIVHLIFLVFIIGGLWKGTQFLPETLHPVHYLPPIAGSFTMASSLSLI
                     GYSEIASLFLGIGIISWLVYEPTMLQRIRLSPLFPIARPSLGIVLAPAFVGASAYFSM
                     GGELDLLAKMLWGYGFLQFCFILRNLKWIAENGFSMGFWAFSFGLAAMARGAIEFYQF
                     EPLSFIGLAGFTIANFGIGLLLIFTLKLWLQQSRA"
     gene            complement(39703..39897)
                     /gene="iscX"
                     /locus_tag="PXD81_000102"
     CDS             complement(39703..39897)
                     /gene="iscX"
                     /locus_tag="PXD81_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005656371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HDL5536177.1"
                     /translation="MKWTDTRMIAENLYDMNPDLDPTTVRFTDMHKWICEMEDFDDDP
                     EASNEHILEAILTIWLEEYE"
     gene            complement(39897..40238)
                     /gene="fdx"
                     /locus_tag="PXD81_000103"
     CDS             complement(39897..40238)
                     /gene="fdx"
                     /locus_tag="PXD81_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006717450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HDL5536178.1"
                     /translation="MPKIVFLPHEEFCPEGMVVEAKAGDNLLEVAHEAGVEIHHACDC
                     SCACTTCHVVIREGFDSLNDTSDQEEDMLDKAWGLEIDSRLSCQCIVGEEDLVVEIPK
                     YNLNHANEAAH"
     gene            complement(40257..40622)
                     /locus_tag="PXD81_000104"
     CDS             complement(40257..40622)
                     /locus_tag="PXD81_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity 51 family protein"
                     /protein_id="HDL5536179.1"
                     /translation="MSNYVKTEISEQFRTVAVEFDLEDNRIFELGERINTEYDVAYMN
                     GYNWAAFLRAYLSIYRPSLLELLEEDPDVGCYYVTYPLSEKSKEKARELKKIIVYLVE
                     NEEEILNFIGEHLDNIEWD"
     gene            complement(40632..42485)
                     /gene="hscA"
                     /locus_tag="PXD81_000105"
     CDS             complement(40632..42485)
                     /gene="hscA"
                     /locus_tag="PXD81_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HDL5536180.1"
                     /translation="MALLQISEPGQTAAPHQHKLAVGIDLGTTNSLVATVRSGQTQVL
                     LDEQERALVPSVVHYAETEKTVGIEAFAKAALDPKNTVISAKRLIGRSLADVQQRYQN
                     LPYQFVASENGLPLIQTAQGNKSPIEVSADILSHLAQFAEKRLAGELSGVVITVPAYF
                     DDAQRQSTKDAARLAGLNVLRLLNEPTAAAIAYGLESGQEGVIAVYDLGGGTFDLSIL
                     RLRKGVFEVLATAGDTALGGDDFDHLLAEWIAEQANYKPQTASEQRELLTLATQTKVA
                     LSQAVEFEVKFANWIGVVSRSQFNELIQPLVKRSLMTCRRALKDAGVEAEEVREVVMV
                     GGSTRVPFVREQVGEFFGKTPLTSIDPDKVVAIGAAVQADILVGNKPDSDMLLLDVVP
                     LSLGIETMGGLVEKIIPRNTTIPVARAQEFTTGKDGQTAMTIHVLQGERELVEDCRSL
                     GKITLRGIPAMAAGVAQVRVTYQVDADGLLNVTAMEKSTKVQASIQIKPSYGLTDEEV
                     TQMIKSSMANAKEDMEARQLVEQRVEADRVIDSVVTALKEDGSAVLSVEEFQAIEAQL
                     ARLIELKEGTDRFAIQQGIKEVDLATQEFAARRMNLSIQKALAGKKMDDLA"
BASE COUNT        13017 a         8943 c         8482 g        12123 t
ORIGIN      
        1 cgtattgaac aagaacttgc agacttaggc attacaaatg taaaagaaat cgtgcgtaac
       61 cctagctatg agcaactgtt tgaagaagag atgaaatcgg atcttaaagg ttatgaaaaa
      121 ggcgtcctaa caaatttagg cgcagtagcg gtagataccg gcattttcac ggggcgttcg
      181 ccgaaagata aatatatcgt attagatgaa acgacaaaag ataccgtctg gtggacatct
      241 gatgcagcta aaaatgataa taaaccgatg actcaatcta cttggcaaag tttaaaaacc
      301 ttagtgaccg atgagttatc taataagcgt ttatttatta ttgatgcttt ctgtggtgca
      361 agccctgagc atcgcatcaa tgtgcgtatt attacccaag ttgcgtggca agctcacttt
      421 gtgaaaaata tgtttattcg tccaacagaa gaagaattaa aagatttcaa accggatttt
      481 gtggtaatga acggttccca agtaactaac ccgaactgga aagagcaagg cttaaattca
      541 gaaaactttg tggcatttaa tatcacagaa ggtgttcaga tcattggcgg tacttggtac
      601 ggtggtgaga tgaaaaaagg tatgttctct atgatgaact acttcttacc attaaaaggt
      661 gtcgcttcaa tgcactgttc agccaatgtg ggcgaagaag gtgatgtggc ggttttcttt
      721 ggtttatcag gaaccggtaa aacgacacta tcaaccgatc cgaaacgcaa attgattggt
      781 gacgatgaac acggttggga cgaaaacggc gtatttaact atgaaggtgg ttgctatgcg
      841 aaaaccatca acctttctat tgaaaacgag ccggatattt atcgtgcaat ccgccgtgat
      901 gcgttattag aaaacgtggt tgtgcgtgaa gatggcacta ttgattttga tgataaatca
      961 aaaacagaaa atacccgtgt gagctatccg atttatcata ttgataatat tgccgtgccg
     1021 tcaaaagcag gacacgctaa aaaagtgatt ttcttaacgg cagatgcatt cggcgtattg
     1081 ccaccggtgt ctaaattaac gcctgaacaa actaaatact acttcttatc aggctttacc
     1141 gcaaaattag caggtacaga gcgtggtatt actgagccaa ccccaacttt ctctgcttgt
     1201 ttcggtgcgg cgttcttatc actccacccg acacaatatg ctgaagtgtt agtgaaacgt
     1261 atggaagcag ccggtgcgga agcctattta gtgaataccg gttggaacgg cacaggtaaa
     1321 cgtatttcga ttaaagatac acggggtatt attgatgcca tcttagacgg ctcaattgag
     1381 aaagcggaaa tgagtacatt accaatcttc aatttagcaa ttccaactgc gttaccgggt
     1441 gtagaaaccc acattttaga ccctcgtaac acttatgaag acaaagcaca atggcaagct
     1501 aaagcagaag atttagctgg cagatttgtg aaaaactttg aaaaatatgc gaccaacgag
     1561 gaaggcaaag cattaattgc tgctggtccg aagttataag ttgtaattat aagttataat
     1621 cgctaaaaaa ttagtttagg actaaacctt gaatgaggtt tagtcctttt gtataaagga
     1681 gaagtacaat aatgacaaca ttccaattaa ggctgaaagc caaagatgag cttaccccac
     1741 acccaacagc acaatattgg cgaaaatgcc aagtggaagg actatttgat ctgccgttta
     1801 tggatttagt gtttagagcg gcacaagttc accgtgaaaa ttttgatgct aatgcgatcc
     1861 agctttcaac gttactctca attaaaacag gaggctgtcc ggaagattgc gaatattgcc
     1921 cccaatctgc ccgctatgat acgggaatag aaaagcaaac aatgcttgat gtagagacga
     1981 ttgtagaaaa agctaaaatt gccaaagagc gtggagcaag ccgcttttgt atgggggctg
     2041 cgtggcgagg acctaggcct aaagatatta aagtagtctc agaaatcatc aatgcggtga
     2101 aatcattggg gctagaaacc tgtggtactt tcggtatgct tgaagaggga atggcagaag
     2161 aattaaagca agcagggctg gattactaca accataattt agataccgac cctgaacgct
     2221 ataacaaaat tatccatacc cgaaatcacg aagatcgaat ggacacttta ggtaaagttc
     2281 gcaatgccgg cttgaaagtt tgctgtggcg gtattgtggg aatgaacgaa acccgccccg
     2341 agcgagcagg cctaattgca agcctcgcta acctcgatcc tcaacctgaa agcgtaccga
     2401 ttaatcagtt gattaaagta gaaggtacac cgcttaccaa tgccgaagat ttagactgga
     2461 cagaatttgt tcgtaccatt gcggtagcaa gaattactat gcctcaaagc tttgtccgac
     2521 tttctgctgg cagaactact atgccggagg caatgcaagc cctctgtttt atggcaggag
     2581 caaattctat tttctatggc gataaattac ttactacaga aaatccggaa gaagatcatg
     2641 atcgccaatt aatggataaa ttagatttat atccgctaca atatatagcc tgagtgccaa
     2701 tgattttcgc ctgaatgaaa attaaagaat caatcgttat tttacgcaaa tttctaacat
     2761 aaaataaccg cttgcaaaat tcgttcttgt cttattttta aatttatcta taatgtgagg
     2821 ccttacgcaa gcttgcgtaa ttcgatgggg cgttattggt tcctcgcaat ggtgttcagc
     2881 ttacttgatc aggttcggaa gaaagcagtc tgggttgaaa tttccgtgtg ccgagggagg
     2941 ctggtaatgc cccacccttt tttcagttct atcaggagaa cgttatgctc cagatcaccc
     3001 ccattccggc attaaaagat aattacattt gggcaatccg taacgggcaa gatgtgatta
     3061 ttattgatcc atcagaacat cagccagcat taaactttat tgcaaaaaat gcgttaaatt
     3121 taaccgctat cttgattacc cataatcacc acgaccatac tgatggggtg agtggtattg
     3181 ttgagcaata tccgaatatc cctgtttatg gtccgcaaga agtggctgaa tttgccaatg
     3241 tgattgtctc gcctgaacaa catatcagcc tgtttggtta tgatgtaagg attattgaaa
     3301 gtggtgggca taccgcaggg catatcagtt atttatttgg ctatgattat ctgttttgtg
     3361 gcgatgcgtt attctccggc ggttgcgggc gggttttcac cggtgattac caagcccaat
     3421 ttgatgcgtt acaacgcttt aaaaatctgc ctaactcggt taaagcctac ccggctcacg
     3481 aatacaccaa gagcaatttg aaatttgcag aagcggttct accaccaagc tgtgtattat
     3541 cggaatatca agaacaggtt gatcttttac gttcgcaaaa caaaccgact ttgccaacca
     3601 ctattggctt ggaaatgcag gtgaacccgt ttatgcaagc ggtcgattta gccgaattta
     3661 ttgcacttcg tcagcaaaaa gacaattttt aacctcatat ttctaattaa gcattaaagc
     3721 ctctcacttt aatgcttttt ggttaaataa aacctttgca aacatcgctt gtcaattcca
     3781 ctctggctta tcctatggta tagttacgac tatttttagc ttaatttatt attttagtga
     3841 gtaaaattct gtgaacttcg atcctcaaaa tatgcctaag cacgttgcca ttattatgga
     3901 tggcaatggc agatgggcta agcagaaagg aaaattacgc atttttggtc atcagaatgg
     3961 tgtaaaggca gttcgttctg cagttagttt tgcagcaaag catcatatcc aagttttaac
     4021 cctctacgca tttagcagtg agaattggag cagaccagaa actgaagttt cagctttaat
     4081 gtctctgttt atgaaagctt tagattctga agttaaacaa ttacataaaa ataatattcg
     4141 cttaaagatt ataggcgata aatccgcctt tagcgaaaat ttacaaaaac gcattgccga
     4201 atcggaaaaa ttaaccgaaa acaatactgg gctaatctta aacattgctg ccaactatgg
     4261 tggttactgg gatattgttc aagctgctca acaactagca gtgcacgtta aatctgaaca
     4321 acttgctgtg gagcagatta caccagcatt gtttcaacaa catttagtga cagaaaacca
     4381 gccgcaagtg gatttattaa ttcgaaccag tggtgagcag cggatcagca attttctatt
     4441 atggcaaatt gcttatgccg agctgttttt tagcccggtt ttatggcctg attttaatga
     4501 tgaggctttt agtgatgcaa tttatgctta tcagcaacgt gaacgccgat tcggtggttg
     4561 ttaatataaa aagaataagg aaatacaatg cttaaagaac gtgtactttc agcgattatt
     4621 atgattattg ccgtgctggc tgccatattt tggctctcac cacttccttt aactttagtg
     4681 ttatcggcaa ttgttgtggc tgcaatgtgg gaatgggctc aatttgccgg ctttaaacac
     4741 cctgtgccac gtgcaattgt ggcaatggtg acaatctgct tattactttt tgcgatcgtt
     4801 gcgaatactg attatatccg agccacccgt tttattactg atgagactac ccctatcctc
     4861 tttattggct gtatttggtg gttaattgcc cttgtcctag tggcaactta ccccaactcg
     4921 gcaaaactgt gggaaaaatc tgttgcagct aaattcctat ttggacttgc taccttagtg
     4981 ccgtttttaa ttggcacgct cgcattgcgt ttccataatt atgcatttga tccataccaa
     5041 ggaacttatt tattccttta cgtctgttta ttagtttggg gagctgattc aggtgcttat
     5101 tttgctggca gagcctttgg caaaagaaaa cttgccccaa aagtatctcc gggtaaatcg
     5161 tgggaaggcg taattggtgg cgtactcacc tctagtttga ttgcctttac ctttttacaa
     5221 cttacaccgg caaatgtatt cggcaaagca ttagccacta ccccatttat tttagtttcg
     5281 gtcggtatgg ttgctatttc agtcttaggc gatttggcag aaagtatgtt taaacgccaa
     5341 gcaggcatta aagacagtag ccagttaatt cctgggcatg gaggtatttt agatcgtatt
     5401 gatagtttaa ccgctgccgt tccgtttttt gctacattct tcttttttgt actatagaat
     5461 atgacgtcca tcattgcatt ttttatttta atttgcgtac tggtttttgt gcatgaatac
     5521 gggcactttt gggctgctcg caaatgcggg gtaaaggtga tccgtttttc gatcggattt
     5581 ggtaaagtct tatttaagaa aaaagataaa caaggaaccg aatttgtttt ttcacttatt
     5641 cctctgggcg gctatgtaca aatgtggaat gatgaaacgg atatcaacgc tccagctcaa
     5701 caagctctta acacaaaatc tgtattgcaa cgtgcattta ttattatagc cggtcctgct
     5761 gcaaatttta tttttgcgat tttggcatac tgggtcgttt ttattgccgg tattccaact
     5821 gttaaacctg ttattgggga aattttacca aacactatcg cctcccaagc aagaattcct
     5881 accgaattgg aaattacaaa aatcggtcat caatctatcc aagattggga aagtgcatct
     5941 ttagcactag ttggttttgt gggcaataaa aatgtaccgt tagagggaaa tatcaacggc
     6001 aactcaacac aaaaatatga actcgattta tctaactgga gtattgactc agccaaagaa
     6061 aacccattaa ccgcattagg tattcgcccc aaaagtgcaa aagtcaccgc tgaaattaaa
     6121 caagttgtcg aaaattctcc tgcaagccaa gtgggtattc aagcaggtga tcgtattctt
     6181 caaattaacc agcagccatt taattggttc aatttagttg aactggtaca agcaggtaaa
     6241 cctattgaac ttaaaattga acaacgaggt cagattaaag accttgtcgt ccaaccagaa
     6301 aaaaaagacg aacgctatat tatcggggta ataccaagtt atgagcctct tgcagataaa
     6361 tatcgtaccg aattaaaata tgatattctt accgcatttt ataaaagtat cgaaaaagtg
     6421 tggtctctta cccaaaccat actccaattt atcggtaatt taataagcgg tgatttgtcc
     6481 attaaaaatt taggtgggcc aatttcaatg gcaaaaggag ccggtgcaac agctgaaatc
     6541 ggcttagttt attaccttag ttttatggct ctaatcagtg tcaatttggg ggtaatgaac
     6601 ttatttccac ttttaccctt agatggtggg caacttgttt tattggctgt tgaagcaatt
     6661 cgtggaaaag cattatcaga aaaaatacaa ctcaaattcc aacaaattgg atttgcattc
     6721 gtgctgagtt taatgttatt cgcctttgcc aacgatataa ttcactttta acaagcggtt
     6781 aaaaaaggtg acttttttgc aaaaagcacc ctgacatcaa aatccataat atggatttaa
     6841 actatattct taataatttt gcaaaacgat cataggataa tttcaatgaa aaaattatta
     6901 ctttcctctc ttttaattgc aaacggtgta gttgctgcac ctttcgtagt aaaagacatt
     6961 cgtgttgaag gtgtgcaacc tacaacaggc gaatctatca tttcatctat tccggttaga
     7021 gtaggtcaaa cagcaacgga tactgacgta tctaatgtcg tccgccatct attcagccaa
     7081 caacgttttg cagatgttcg tgcaacacga gaaggcaata cgcttgtcat caaagtcgct
     7141 gaaaaaccga ttattggcaa ggtagagatc gaaggtaacc aagcgattcc taaagatgcc
     7201 ttagagcaaa atttaaaggc aaacttaatt aaccaaggtg aaatttttga tgctgccaag
     7261 cttgaagctt ttaaagaagg actaatcgaa cattatcact ctaccggccg ttatgaagcc
     7321 aaaattgaca catctgtttc gaataacacc gaaggtggcg ttaatattaa gttagcgatt
     7381 aatgaaggtg aagttgctaa agccaaaaca attaagtttg aaggtaataa tgccttctct
     7441 gataaaaaat tattagaaaa cttagatatt caacctgatg tttcttggtg gaatattttt
     7501 gaaagtagca aattcgaaca aaccgcctat caaaaagact tagaaacatt aagagatttt
     7561 tatttaaatc gtggctatgc aaaatttgct atccaagata ccgatgttca attcaatgat
     7621 aataaaacgg aagttgcatt gacctataaa ttagatgaag ggtcacagta ccatgtcagt
     7681 gaaatgcgca ttatcggtaa tacagccaat atggatagcc agctcaacgc cctactaaaa
     7741 gaattcaagc ctggtcaatt attccgtaaa tcagacctta cacaaattga agaagagatt
     7801 aaatctattt taggggacgc aggttatggt tcagctaaag tagatattta tcctcaattt
     7861 aatgaagaaa ataaaaccgt aaaaatcagt tttgttgtgg atgccggtcg tcgtatttat
     7921 gttcgtaaaa tccgttttga aggtaatggt gtcactgcag attcaacatt acgtcgtgaa
     7981 atgcgccaac aagaaggtgc ttggctttcc acaaacaaag cttctctagg taaagctcgt
     8041 ttagaaagaa ccggtttcta tgaaagtgtt gatatgtcaa tgccaaatgt agaaggtaca
     8101 gccgatcaag ttgatgtagt gtataaaatt aaagaacgca ataccggtag tattaacttt
     8161 ggtatcgggt acggcacgaa aaacggtatc agctatcaag caggaattaa acaagataat
     8221 ttcttaggta tggggtcaac tatcagtcta aatggtaccc gtaataacta cggtaccagc
     8281 gttaacctag gctataccga accttacttt actaaagatg gtgtaagttt aggcggtaat
     8341 attttctatg aagattatga taactcagac aatgattcaa tggcttcata taaacgccaa
     8401 acctatggta ttaacggcac attaggcttc ccggtaaatg aaaataactc atactactta
     8461 ggtttaggct atacgcacga taaaattaaa aatgcagcac gtgagtacac tcgtgagaaa
     8521 tatgtaaaat caatgaactt tgagttcgat ccagctgccg attattacaa aaaaatcaaa
     8581 gctgatgatt ttgatttttc tctcggttgg aactacaaca gcttgaatag aggctatttc
     8641 ccgactgaag gaactaccgc aaacattggc ggtaaaatca caattccagg ctctgataat
     8701 aaatattacc gagtcagtgc agactttaga aattattacc cgttaaaccg tgagcacaaa
     8761 tgggtaattt caacgaaagc tagcattgct tatactaatg gatttggtgg taaagaagtt
     8821 ccgttctatc agctttatac tgccggaggt attggtacac taagaggttt ctcttacggt
     8881 gcagtagggc ctcaagcgat ttactataat acaaaatccg gcaaatttga tagcatgaat
     8941 aatgatatta tcggtggtaa tgcgttagct gcggcaagct tggaattaat cacaccaacg
     9001 ccatttgtga gcgaaaaata tcagcacaat gtaagaacct ctctctttgt tgatgctgca
     9061 agtgcatgga atacaaaatg gaagaaagat gaatatacga acttgcctga ctatggtgat
     9121 ttcaaacgtt tccgtgcttc cgccggtatt gcattccaat ggatgtctcc aatcgggcca
     9181 ttatcattct cttatgcgaa accaattcgt aaatatgata atgatgatat tgaacaattc
     9241 cagttcaaca ttggtagttc attctaatta accacaagcg gttaataaat gtaaaaaatt
     9301 tgcaacttag ccgcttattt caaaactgtt catacaagga aaacaaatga aaaaattatt
     9361 taaaatgact ggcttagccg tagctttagc ctctacaacc ggaatcgcga atgctgcaga
     9421 tactattggt ttcgtagatc caagctatgt attacaaaac caccctgtat tattagatgc
     9481 ttcggcaaaa tttgataaat tcatgaaaga aagccaagaa aaatttgctg ctgatgaaaa
     9541 aaaattagca gatgaaaata aaaccttaac ggctgagcgc aataaaatta atgaagatgc
     9601 gcaaaaatta caaaatgaac aaaaaaatgt agaagcttct atcaagaaaa aagtggcagc
     9661 attagagaaa gatgcgcctc gcttacgttc aaaagagatt caagcacgtc aaaatgcgat
     9721 tcaaaaagaa gcaaacgcat tccaagctaa agtaacagca attcaaaaac gtgaagctga
     9781 ttttgcaaaa aaagcagaag ccttccagaa acgtgcagat gaattccaaa aacgtattga
     9841 acaagcgaat aaagaaaatg gtggcattaa cccacaagaa gcgcaaaaac aagcggttga
     9901 tgaagtaaat gcaaccatta aagagatcgc aaaatcgaaa ggttacactt tagtattaca
     9961 accacaagta gcactttatg ctgaagatga aggtaaagat attactgaag cagtacttga
    10021 tgctattgtt aaaaaacatc cggaaattaa aatagaaaaa cctgcaactg aagctcaacc
    10081 agctacagaa aaagaaacca aagctgaaga aactaaacca gaagaagcga aaaaataatg
    10141 gcaaatttcc gtttaattga tttagcggag catatcggcg gtactcttag gggtaacgcc
    10201 gatttggcta ttcaaagtat tgctgcttta gataaagccg acagctcgca aattaccttt
    10261 atctcaaatg caaaatatcg ggaaaatttg accgcttgca atgctggagc tattatcgtc
    10321 agcccggcag atgtagaatt ttgccgagca gatcaaaatc ttatcatcgt tgccaatccg
    10381 tatgttgctt atgctcaact ggctcaatat atggattcta ctccgaaagc agcgagcgaa
    10441 atccacccaa gtgcggtgat ttcacctgaa gctaaactgg gtaataacgt atcggtaggg
    10501 gcaaatgccg tgattgaaag tggcgtagaa ttgggcgatg atgccgtgat cggtgcaggc
    10561 tgttttgtgg gaaaaaacag taaaatcggg gcaagaaccc aactttgggc aaatgtttcg
    10621 gtgtaccaca atgtacaaat cggagctgat tgcttgattc aatcctcaac ggttatcggg
    10681 agtgatgggt ttggctatgc gaatgacaaa gggcaatgga ttaaaatccc acaaaccggt
    10741 ggtgtgatta tcggtaatcg tgttgaaatt ggtgcttgca cttgtattga ccgaggtgca
    10801 ttagatccaa ccgtaatcga agataatgtg attattgata acctttgcca aatcgcccat
    10861 aatgtacaca ttggttttgg cactgccgtt gccggcggcg tcattatggc aggcagtcta
    10921 aaagttggtc gcttttgcca aattggtgga gcaagtgtta tcaacggtca tatggaaatc
    10981 tgtgatggtg ccataattac cggaatgggt atggttatgc gcccaattac agaaaaagga
    11041 atttactcat caggtattcc attacaaacc aataaagaat ggcgaaaaac cgcagcactt
    11101 gtaatgaata ttgatgaaat gaacaaacgc ttaaaatcgt tagaaaaacg ttttaacgat
    11161 taattttatt agaggtctta aagtgacaga aatgacaact gaaaatcgtg agccaaatat
    11221 tattgaagta accgaaatta tggatatgtt accgcatcgc taccctttcc tgttagttga
    11281 tcgcgtaacc gattatgaag aagggaaatg gttaaaagcc attaaaaatg taaccttcaa
    11341 tgaaccttgt tttaccgggc attttcctgg cgcaccaatc ttcccaggag tattgattct
    11401 ggaagcaatg gctcaagcaa ctggcgtatt agccgttgcc acttatggta agttaagtga
    11461 agacgaactc tactattttg ccgcaattga caatgctcgc tttaagcgac cggttgtgcc
    11521 gggcgatcaa cttcaacttg aggttgaatt tcttaaagaa gtacgtggta ttactaaatt
    11581 taccggtaaa gcatacgttg atggcaaatt agtctgtgaa gcagatttaa tgtgcgctcg
    11641 ccgtaaataa ttcgatataa gcccaaacta gagggaacaa actatgcgtc taattgattc
    11701 aacagcaaaa attagcccgc ttgccatcat tgaagaaggt gctgaaattg gcgctcacgt
    11761 tgaaattggt ccattttcag tcatcggtaa agatgtcaaa atcggggcta gaacgaaaat
    11821 tcactcaaat gttgtcatta acggcatgac tgaaattggt gaagataacc atattttcca
    11881 atttgccagc attggtgaaa tcaaccaaga tctaaaatac caaggtgaac caaccaaagt
    11941 ggtgatcggt aatcgtaacc gtattcgtga aagtgttact attcaccgag gtacggtaca
    12001 aggcggtggc gtgacgaaaa tcggtgatga taacttattt atgatcaaca cccacatcgc
    12061 acacgactgc tctatcggta accgttgtat tatcgccaat aatggtacgc tagcaggtca
    12121 cgttacccta gatgatttcg tgattgtggg aggaatgtcc gccattcacc aattcgttgt
    12181 gattggttca cacgttatgc ttggaggcgg ttcaatggtc agccaagacg taccgcctta
    12241 tgtcatggca caaggtaacc acgcacgccc atttggtatt aactttgaag ggttaaaacg
    12301 tcgtggcttt gataaaccaa ctatgcacgc tattcgcaaa gtctataaac tgatttactc
    12361 cagcggaaaa acattggaag agtgcctagt agaaattgag caaattgcag caacagaacc
    12421 tgcgattgct atttttaaac aattcttcaa acgttctacc cgtggtatta ttcgttaata
    12481 aacgaaaccc cgaaatcaat tcggggtttg ttttatcaag cgaccgtcga acctttcaac
    12541 aattttcgaa tccaagctcg atttgggcta aggttatgcc aaatatcctc actgattggc
    12601 acaggctcat tcacaatcag gcttgctaac aattccccta gaagtggtgc agtcgtcaaa
    12661 cctcgagaag acaggccgtt taccatatac agattggtaa aaatttcagc attttctacc
    12721 gcttgtttcc gtctcaactg gttgtataaa ttggcatatt ggcttttttg ctgctcaaaa
    12781 tgatgcactg ccccaaccat cggtacacga tcacgaaacg ccgcacgaat accgactttg
    12841 gctttatttt cagataaatc aattccttgc gtccactcac aaacggttac attttgctga
    12901 agttttgcaa cattctccca atgctccgat aaactaaact cggtttctgc attatcccga
    12961 atatggcttg ctccaatgca atgcgtgcca taggctgaca ttggcgttaa ataaccatca
    13021 taacaaagca cacacttcag tttttgtaat tcgcttgtag tcggaatttg gctcacttgc
    13081 cctcgtaccg gatacaacgg aatgccgttc gcttggctaa attcattcaa cgtatgtcca
    13141 tttgccagca caaccgcttg atgtcgaacc gtttctcccc tcgtctgcca ttgccacatt
    13201 ccattagaaa aggttaaatg ctcaactttg tgattcagta caatcttcaa ccctttctgc
    13261 tccagccaag taaagccatt tctaacaagt tgtaccggtg aaagccaacc agcttgcgga
    13321 ataaatgccc cgctgtttgg cacgcttaaa cctaattttt cacttaattc tgcctgtgaa
    13381 caagatttta ataaggtgtc atcagccgtc tgctccacca ttttttccag ttttttagcc
    13441 gttttctcgt cataggcgta aagcactacc ccgcttaaat tatgctcaaa ctcaatgttg
    13501 gatttcagtt gttgcaagcg ttgcaatgca taatcaaaac tatggatata aaactgtaca
    13561 tttcgctcgt catcatcgct taactgtggg taaatcgccc cttgtaaatt tcctgaagca
    13621 tttattgcca aggtttcatc tttgcaatac agcgtgactt gcttgccttt ttctaacagc
    13681 gataacgcaa cgaagaaact cgctactccg ccaccaataa ttgcaatgtc atcagcggtt
    13741 tcatttttgg cataaaaata ggggtaatta ggctcggaaa cttggctatc taacggtttc
    13801 tcgccccaaa gcatttctcg ttttttgcca aatcctttgc gttttttcac tgcaaaacct
    13861 gcggcttcca gccctcgtct gaccatactt gaagcggtaa aagtagcaaa actgccgcca
    13921 ttggcactta gtcgaaacat ttgcagatat aactgttcgt tccacatttc tgggttttta
    13981 tcaggcgaaa agccatctaa aaaccaagcg tcaatcgtgc cgctataaag atcgcctaat
    14041 tggggtaagt tttcagcaat ctcaccgaac cataaatcta aataaacctg ctcaaaatga
    14101 taacgttggc aacctatttg gcgaggctgc caacaagcgg ttaaattttc agataatgtt
    14161 gcaaattgcg gatagcgttg gtgaatatca gctaattggc tggccgtcag aggaaatttc
    14221 tcaaatgaga taaaatagag tcgttttagt ttactttgcg gaaatgctgc ccgaaactgt
    14281 tggaatttgt cagcaacggc taaaaaattc aagcccgtgc caaagccggt ttccgcaata
    14341 acaaaagcct ctttcgggtg agattgccac ttttcccaca gttggttacc ttcttgaaac
    14401 acatagtaac tttccgccaa accatcttgg gtagaaaaat aaatatcatc aaattgctcc
    14461 gacaccggtg tatctgcatc attaaatgta agtgtcgcaa aagtcagttt acccatcata
    14521 tccccttaaa aacaaaacac ggaaaacccc aagattttcc gtgtttcaac cagttcaata
    14581 gtaccaatgt tagaaaatgt tgctggcaaa aataacgatc ataccaagtg cgcctacaac
    14641 cgttagcatt gaataaccaa aagtacccat tttacaatcc tcattgttaa ttatgattaa
    14701 aacggcttta ttctagcata atttccatcg ctttgaatgc ctatgcttga ataattttgc
    14761 caaaacaagc ggtcaatttt tgtaaatctt ttacgccttt ggcactttcc acgccagaat
    14821 ttaaatctaa cccgacacag ccttgagcaa gggcttgctc aatattctca gtgccaatac
    14881 cacctgccaa taacgctttt tgttttaagg tttctggaat caacgcccaa ttaaaggttt
    14941 tgcctgtgcc gccttgctga ttgcctactt tgctgtctaa tacataacga gcaatctgtg
    15001 gattatcgtt aaattctaga ttttcaacct caacatcaac cgataaggct ttccagattt
    15061 gagttttact acctaatttc tctgccagtt ctgcaatgta ttgctcatct tccgagccgt
    15121 gcaattgaac tgcaaataac tcaagctgtt ttgccatttt ttccacaaat tcgaccgctt
    15181 gattttggaa cacccccaca taacgcaacg gtgcattcac caccaattct tgggcttggc
    15241 gaagtgaaag ctggcgaggc gagccttcgg caaaaattaa accgccatat aatgcccctt
    15301 gctcataaac cgctttcaca tcttgcacac gggttaagcc acacactttg ttttcaccaa
    15361 aaatcaccgc ccgcaccgca ttattgagat cggcacttcc cattaaactg ctgccgatta
    15421 aaaaagcatg aacatacggc ttaatgtttt tcacttgctg gtgatcgtta atacccgatt
    15481 ctgaaatcaa acagacatca gccgaaattt gggcttggta tttttccacc aaacgcacaa
    15541 tgcggttcat atcaatcgtg agcgtgtgca aatcacggtt attaacgcca attaccttcg
    15601 cccctaaggc taaggcacgt tcaaattccg cctccgtgct ggtttcagtc aaaacgccca
    15661 ttcctaactc gtgagccagt gtggcaagtt cacggtaggt ttcatcattt accaccgata
    15721 acatcaataa aatcgcatca gcattataaa aacgggcaag gtaaacctgg tatgccgaaa
    15781 tcataaaatc tttacacaaa atcggctgtg tggtttggcg tttgacttga tcgatataac
    15841 ggaaatcgcc ttgaaaatat tgctcatcgg ttaaaaccga aatcgccgca gcgtaatttt
    15901 tatacacttg ggcaatactg tctaaatcga actcagcacg aattaaccct tttgacggtg
    15961 aagctttttt acattctaaa atataagctg gttcttggtg tgtgcctttg gcaagagccg
    16021 catagaaatc atggtcgctt tttacaattt tgtgctgaaa ttcacttaac ggaaaggctt
    16081 gctctttttg tgccacccaa atggctttat cttgtacgat cttttgtaaa atcgtcggtt
    16141 ggtttggttg agcgttatct tgaacgatgt tttgcatata aattcctaga gcgaaatcgg
    16201 tttgaatggt aaaatctgcc cttattttta cccgaaaaag tctcatttca caagcaaaac
    16261 taagatgaaa tcacaagtta tacagcattt atcttattta gaaatcgccc tgagagttaa
    16321 tcaactttgg gaggcgacac caccaagccc tgaagcctta gccaatgagc aacctttctg
    16381 cgtggaaacc ttaaccccaa cccaatggct acaatggatt tttattccca gaatgcgggc
    16441 cattttagat gcaaatgccg atttacctcg caattttgca atcacccctt atttagaaga
    16501 ggcattgaaa aacgaaggtt atttacaagc gatttgtgaa ccgctattag gtttggaaaa
    16561 gttgttgaaa gattaacttc tctctcagtt cttttattaa tctgacaaat tcgttgatag
    16621 tgccagcgtc cacgctggta ctaattttaa cggttaatta atcaaaattt ggcacaagcg
    16681 aggacgcttg cgccatcata gagatttgat aaattatact taatgtatta tgaccttaga
    16741 tattttatac caagacaact cgcttatcgc catcaacaaa cctgccggag tgttggtgca
    16801 tcgttcttgg ttagataaac acgaaacggt gtttgcaatg caaaccctga gggatcaaat
    16861 cgggcaacac gtttttccga ttcaccgcct tgaccgccca acctcaggtg tattgctgtt
    16921 tgctctcaac agcgaaaccg cccgaataat gagcgagcag tttgaacaac acctagtaca
    16981 aaaaacttat ttggcggtgg tgcgtggcta tttacaaggt tcggcacaaa ttgattatcc
    17041 gctcaaagtg attttagata aaattgcgga taaattttct caggaaaaag aggctcaagc
    17101 ggcgattact gattataaag gcttagcaac ggttgaaatg ccttaccctg tcggcaagca
    17161 ccaaacctct cgttatagtt tggttgaact tagccctaaa accgggcgga aacatcagct
    17221 tcgccgccac ctgaaacacc tgttccaccc gattatggga gacagcaaat atggcgactt
    17281 acaccaaaac cgtgctttag ttgaaaaatc aggcgtaagt cgtttaatgt tacacgccca
    17341 taaattgcaa tttcagcacc cgaaaaattt gcaaaaaata gagataattg catcgcttga
    17401 tgagcaatgg caacgcctct ttgcattttt tgactggaac tttactcaat attattagac
    17461 aaaaaagagg attgaatgga taattcactt ttatcattga cacacgagca acaacaagcc
    17521 gcagtagaag aaattcaaaa actgatggca caaggcatca gtgccggaga ggcaattcaa
    17581 attattgcta atcaattacg tgacaaatat caaatattgg agaaatcaaa caatgaataa
    17641 attatggaca gtgcttgccc taacaataac ttcccactat agctttgctg ccgctgaaaa
    17701 aatagtcact aaagatttac cggcagaagg aaaacctgca gtcgtgctac aggtttttga
    17761 tgaaagtgaa aaaattgcaa aacctattaa gggaaataag ctctttatta gcaaaaaaca
    17821 aacaaggctt tgctggcaca gtattaatct tcctgtacaa aataaagtta ctattgcaga
    17881 agctttctat gcccctgctg caatgaaatt agcttctccc ggctctaaaa ttaattcttc
    17941 tgcagacaag aaaaatcata ctatcattac tgaaataaac cttgcaaata atcaagtatt
    18001 aaatcgttgc tggggttttg ataaaactga ccctgtcggt aaatacaaaa tggaaattca
    18061 aattaacgat cacattttca aaggtttaga atttgaaatt gtaaaataac ctaaaagcca
    18121 cagagtaaca ccctgtggct ttttgctaaa tcattgtttt taaattggtt aaattgtgca
    18181 gcaaataatc tttactcttc acaaaggctc tcgcctgttg ccagttgcta aattgttgct
    18241 gagtgcattg attaaaacgt accagcactt ccaaactttc attgccgaca caaataaaat
    18301 cagctaataa ttgggttaat ttttcactgt tttcaccatc cattgcagtt gcaattttct
    18361 gctcccattt aaccgcttgt ccgagcaaat aaccccgctg ggctttgctt aaattactca
    18421 accacatccc atccgctttt ggcattgcct caaccaaagt aaataagtcg cctagatttc
    18481 cttgcttgat ttcaaactcc aattcacaaa tcggctcttc accaaaatga tttttaatga
    18541 tgccctgatc taacgctacc tcaatgtcag acatccccac ttgcaccaac caagttttac
    18601 gagtgaaatc ggtgctgaat atcgcttgca agctttctgc aatctgctct gaattttcaa
    18661 acggcaactg gtatttttcc actaatgttt gaaaatccgg ctggctattg ggtaaagcta
    18721 aattatattc cggacgaata tgcaaaccgc ctacaatttc cccttgtgtt tttaacgtga
    18781 tttcatattg ttgattcaca gaacgcaccc gcagccccat tttttgcgtg gcaaaaaatt
    18841 gctcgggcgt atcataatag gtgttgccta acaattccgt ttgatgagca agcacaggga
    18901 atggcgaggt ttgattcaag tagttttcca ctaacgccac attctgctgt tccaacatta
    18961 ttttcagttc gatttctgct tgtttttggt tttccatttc acacgcccct gcaacgttgt
    19021 ataatcaagc ggttatttta tcaataaatt ttgcaaaatg ttttcaaaaa ttcaccataa
    19081 actgaataac tttgtccagc gtggactcga caaccattta cgcctcgccg ttaccggctt
    19141 aagccgtagt ggcaaaacgg catttattac cagcttggtt gatcagcttt tgcatatcaa
    19201 caaaggcggt aattcgcatt taaacctgtt tgcgcctgca cgccacgggc aaattctatc
    19261 ggtaaaacgc gttgagcaag gggatttaac tattccccgc tttgaatatg ataaaaaccg
    19321 ccaaagcctt gaaaatcaac cacctcaatg gcctgaatcc accactggca ttagcgaaat
    19381 tcgccttgcg attcgctacc agcggcaaca ttctctgcta cgtcatatta aagaaaccgg
    19441 cacgctttat ttggatattt tcgattaccc gggcgaatgg ctgttagatt tacccctgct
    19501 ctccctttcc tataaagaat ggtcgcaaac gcaacaaacc atacataaag atgagcgtgc
    19561 agagcttgcc acaccttggt tacaagcggt taaaaaactc gatctttttg caaaaaccga
    19621 tgaaaattta ctggcagaat tcagccaaca atacaccgat tatttgctcg cctgcaaagc
    19681 tcaaggaatg caatatattc agccggggcg atttgtgttg ccaactgaaa atgccaaagg
    19741 tgcaccggta ttccaatttt ttccgttatt agatttaagc gaagctgagt gggaaaagtt
    19801 ggaacaaagc gacagaaaca gtattttcca taccctcaaa aaacgctatg agcattacca
    19861 gaaaaaaatc gtcaaaccgt tttatgaaga ttacttctct caatttgacc gccaagtgat
    19921 tttggcagac tgcctcacac cgttaaatta tggctataac gaatttatgg aaatgaaatt
    19981 agggttacag caactgttta aacatttcca ttatggcaat cgttcgctat ttcaccgtct
    20041 gttttcgtcc aacattgata aattgctatt tatcgcaaca aaggcagatc acattactag
    20101 cgaccaagtg ccgaatttgg aaagcctaat gcgacaatta gtgcaagaag ggggaagaca
    20161 cgcagaattt gatggtatcg aaaccggcta tttagccatt tcctcgattc aagcaaccga
    20221 tgcggtgaaa gtgactcagc aagataaaat cattaaagcg ataagaggga tccgttccag
    20281 cgatagaaaa caagtgacac tctaccctgg cagcgtgcca aatcgcatac ccgataaaaa
    20341 ttactggcaa tttaaccgtt ttgaattcga ccaattcgag cccaagcaac ttgattatag
    20401 ccaagcgatt ccacacttca gaatggattc ggtgctacaa tttttattgg cggatatgtt
    20461 tgagtaaaat cttgttgcat atttccatta aaggtataaa attttataca ttatgaatac
    20521 tgtaatttcc caacgaaaac caattcactg ggttggatca accttagatg atattcgtga
    20581 atttccggat aacattaaaa gagaactcgg ttttgatctt gatttagttc agcaaggctt
    20641 attaccgagg gattttaaag cgatggcaaa tttgggttca ggcgtaatgg aaattcgagt
    20701 aaaagatccc tcaggggctt ttcgcttagt gtatttagct aaatttaaag atgcaattta
    20761 ctgtttacac gctttccaaa agaaaacgca aaaaacagcc cctaaagaca ttgctgtcat
    20821 taaagcacgc tatgctgccg taaaggagtt agaaaatgaa taatattgat actaaaattc
    20881 gccatactac agaagctcag ggtaatgttt ttgaagatct tggtttccct attgatgaag
    20941 ctcaaaaatt gaaatctgcc agtcagcaac tgattgaaac taaactgatg ctaatgaatg
    21001 aaatgagcaa ctggattgat aaaaataatc tcaaacaatc agaagcggct tctattctag
    21061 gtgttagccg ccctcgaatt tctgatatgg tcaatggcaa gttagaaaag tttaccctag
    21121 ataccttagt cacttttgtt gcaaaaaccg gcaaaacaat ccaattgcaa ttatcctgat
    21181 atttgcaaaa ttttactcaa attcaaccgc ttgttgcttt tatcattcac taacaaggtt
    21241 atttcaagta aaattgtgac taatttttta ttaataaaca caaattatgg caaaaaaacc
    21301 taaagttgca tcaaatacga ttgcgttaaa taaacgtgcc agacacgaat atttcattga
    21361 agatgaaatt gaagccggct tagagctaca gggctgggaa gtcaaagcat tgcgtgccgg
    21421 aaaaggcaat atcagcgata gctatgtcat ctttcgccac ggcgaagcct tcttattcgg
    21481 ggcaatgatt acgcctttga atatggcatc aacccatatt gtcagcgacc cgacccgcac
    21541 ccgcaaactt ctactgaata aacgtgagtt agactcactg ttcggtaaag ttaatcgtga
    21601 aggttatacc attgttgcca cctcaatgta ttggaaaaat gcgtgggtga aagtaaaaat
    21661 cggcttagcc aaagggaaaa aactgcacga taaacgtgaa gatattaaag accgtgaatg
    21721 gcaagtggct aaacagcgta ttatgaaaaa cgccaatcgt ggctaaatga ataattaccc
    21781 tctccccttg tgggagaggg acagaaaaat tgcgttcagc aatttttcag ggaaaggggg
    21841 atttgtaaaa gttcaaggaa aaacaacgct tgccctctct ctgaaaaata tttctgaacg
    21901 aaatattttt cggtctctct cccataaagg gagagagtga aacacaaatt taaactaagg
    21961 aacatacaat gatcgaccaa aacctacttc gtaccaattt agaagacgtt gctacagctc
    22021 ttaaaatcaa acgtaatttt attttagatg tagagcgagt aaaaaccctt gaagaacagc
    22081 gtaaaacgct acaagttaaa accgaaacct tacaagcaga acgcaacact cgctccaaaa
    22141 acatcggtgc ggcaaaagct cgtggcgaag atatttcagc tttattagcc gaagtagatt
    22201 caatgggcaa cgaattagac acggcaaaag tagaattgga taaagtacag gcagaaatcc
    22261 gtgagttgtt gctttctgtg ccaaacttac cggcagatga agtaccactc ggtaaagacg
    22321 atagcgaaaa cttagaagta ttacgttggg gcgaaccacg tcaattcgac tttgaagttc
    22381 aagatcacgt tacccttggc gaaaacttaa acggtttaga ttttgctgcc ggtgtaaaat
    22441 taaccgccag ccgttttgtg gtaatgaaag gtaaacttgc ccgcttacac cgtgcattat
    22501 ctcaatttat gttggattta cacactgaac aacacggcta tgtggaaacc aatgtgccat
    22561 ttttagtcaa tcacgacact ctatacggca caggtcagct accaaaattt ggcgaagatt
    22621 tattccatac tcaaccatta acagggcaag atccaaacga ggtgcaacgc ccattcggtt
    22681 tgattccaac cgctgaagtg ccagtaacga acttagtgcg agatgaaatc attgatgaag
    22741 acagcttacc actgcgttat accgcccaca ccccttgttt ccgtgcagaa gccggttctt
    22801 atgggcgtga tactcgtggt ttaattcgta tgcaccaatt tgaaaaagtg gaaatggtgc
    22861 aaattgtcgc ccctgaaaaa tcaatggaag ccttagaaga attaaccggt cacgcagaaa
    22921 aagtcttaca gcttttaggc ttgccatacc gcaaagtgct actctgcact ggtgatatgg
    22981 gctttggtgc agcaaaaacc tacgacttag aagtttggct accggcacaa aacacttacc
    23041 gtgagatttc ttcttgctca aatatgtggg atttccaagc ccgccgaatg tctgcacgtt
    23101 gtaaagctaa aggcgacaag aaaactcgct tggttcacac cttaaacggc tcaggtttag
    23161 cggttggtcg cacactggtc gctgtgttag aaaactacca aaatgccgat ggctctatta
    23221 ctgtgcctga ggtgttaaga ccttatatgg gtggtttgga agtaattacc gcttaagatt
    23281 aataaatgaa accaatctca gaaatggggt tggttttttt aataaatctt atccctaatc
    23341 tttttcagga taattcataa aaatttcagt aactactcgg cggttttgca ctgtatctaa
    23401 acgaatagga tttttcattc tccctaaacg aaatgcaata atggtgactt ttttcggttc
    23461 atctatttta tctaattgat agaataccat tatttcccgt tgataaaccg ctcgctttct
    23521 gaaacgtaaa tccttgcgaa atgcgacacc tgcttttgga taaaactgta aaccattgat
    23581 gatattcgtt tgagtatgct ccgaaaataa tccatagaaa ataaactcta atagattatt
    23641 tttaaaatca acactaacca tctaactgct cccaagcagt taaatctcct cgtgataaac
    23701 gttcagcaat atcagcataa aattgtttcg gtacttccct ttttacttgg tattgagctg
    23761 attgttcttc taataaatgt tcttctatca tcttgatttc ttctgttgtc cactcttcat
    23821 cttctttatg tacaacaaaa tcggtatcta caatcacaat agacatacct agctccttca
    23881 agtactggtt atatattctg tattttgcct gttctaaatt cagctgtcta gcttttctgt
    23941 tactcacaat ttgcaaattt ttcatcaaat tccaccgctt gccattcaaa aaaaggcgtt
    24001 tttgcggtat cctatcggca attttttcag ttaaatttaa aggttaaatt ttatgcaaca
    24061 gcagtataac ccaagtgaga ttgaacctaa ggttcagcgt tattgggcag aaaataaagt
    24121 ttttaaagca gttaaagacg ttaccaaaga gaaatactat tgcctttcaa tgttgcctta
    24181 tccgtcaggt aagctacata tggggcacgt tcgtaactac accattggtg atgtagtgtc
    24241 tcgttatcag cgtatgatcg gtaaaaatgt attacagccg atgggttggg acgctttcgg
    24301 tttaccggcg gaaggtgctg cggtgaaaaa taataccgca ccggcaaaat ggacttacga
    24361 aaacatcgaa tatatgaaag gtcagcttaa aatgttgggc ttctcttatg actgggatcg
    24421 tgaagtcacc acttgccgtc cggaatacta caaatgggag caatggttct tcacagaact
    24481 ttataaaaaa ggcttagtgt ataaaaaaac ctcaaccgta aactggtgtc cgaatgatga
    24541 aactgtgctt gccaacgagc aagttcacga aggttgttgc tggcgttgtg acaccccagt
    24601 ggaacagcgt gaaattccac aatggtttat caaaatcact gactatgctg aagaattact
    24661 caatcagtta gataatctcc cacagtggcc ggatcaagta aaaaccatgc aacgcaactg
    24721 gatcggtcgt tccgaagggg tagaaatcac ctttaaaatt gccggttcaa acgtagaatt
    24781 accggtttac actacccgtc cggatacctt cttcggcgta agctatgtgg cgattgctgc
    24841 ggctcaccca ttagcagaat tagctgcaca aaataacccg gcattggctg aatttatccg
    24901 tgaagcgaaa aacaccaaag tggcagaagc agaattagcc accatggaga aaaaaggaat
    24961 ggcaaccggc ttgttcgcca ttcacccgtt aacaggaaaa gaagtaccgg tttgggttgc
    25021 taactttgtg ttaatgcact acggtacagg tgcggtaatg gcggttccgg ctcacgatga
    25081 gcgtgacttt gaatttgcac aaaaatacga tttgcaaatt aatcaggtaa ttcaaccgct
    25141 tgatggctca gagtgggatt tcagcaaagc cgcttacacc gaacacggta aattaatcaa
    25201 ctctgccgag tttgacggct tggaatttga tgtggctttc actgcgattg ccgataaatt
    25261 agaggcaatg ggtgtaggta aacgccaagt gaatttccgt ctgcgtgact ggggtgtttc
    25321 tcgtcaacgt tattggggtg caccaatccc aatgatgacc acagaagagg gcgaagtggt
    25381 taccgttcca ttacaagatt taccggtgat tctccctgaa gatgtggtaa tgaacggcgt
    25441 gcaaagccca atcaaagccg acccagagtg ggcgaaaacc acttacaacg gcaaaccggc
    25501 attgcgtgaa accgatacct tcgatacctt tatggagtcc tcttggtatt atgcacgtta
    25561 tacttgccca caatatcacg aaggaatgtt agattcagac gaggcaaact actggctacc
    25621 ggtcgatcaa tatatcggcg gtattgaaca cgccacaatg cacttgctct acttccgttt
    25681 cttccataaa ttgttgcgtg atgcgggtat tttaaattcg gacgaaccgg cgactaaact
    25741 gttatgccaa ggtatggtgc ttgccgatgc gttctactac accagcccaa ctaacgagcg
    25801 tatttgggtc agcccgacac aagtcacctt agaacgtgat gagaaaggtc gtatcatcaa
    25861 agcaaccgat ccacaagggc gtgagctggt tcacagcggt atgactaaaa tgtcgaaatc
    25921 aaagaacaac ggtatcgatc cgcaagaaat ggtggaaaaa tatggtgctg acacagttcg
    25981 cttgtttatg atgtttgcct cccctgcgga aatgacatta gaatggcaag aatccggcgt
    26041 tgaaggggca aaacgtttct taggtcgtgt ttggaattta gtttatagct atgcccaaaa
    26101 tccggcaacg actgcattag atgtgaatgc cttagacaag gatcaaaaag aacttcgccg
    26161 tgatgtacac aaaacgattg ctaaggtgag tgatgatatt ggtcgtcgtc aaaccttcaa
    26221 tacagcgatt gctgcggtga tggaattgat gaacaagctc accaaagccc cacttgaaac
    26281 cgaacaagat aaagccgtaa tggcagaagc tttaagtgcg gtggtgcgta tgctttaccc
    26341 aatcacgcca catatctgct ttgagttatg gcaagtgtta ggtaatgaac aaaccatcga
    26401 ttttgcccct tgggtacaag ccgatgaatc cgcaatggtg gaagatgaaa aattagtggt
    26461 agtacaagtc aacggaaaag tgcgtggcaa aatcactgtt tcagctactg ctacggaaga
    26521 tgaagttaaa gctatcgcta aagccgatcc aaacgttgct aaattcttag atggtcagca
    26581 gattgtaaaa gaaatctacg ttccatttaa aatgttgagc tttgcggtta aggcgtaaca
    26641 aacacactct cccttatatg ggagagagac cgataaatat ttcgtgcaga aatatttatc
    26701 agagagaggg tacaagcggt tattttcttg gaaaattttg caaaaaatag ccccctctcc
    26761 ctgctttttc ttctaaacga agaaaaacct gtccctctcc cacaagggga gaggggaata
    26821 aacaaggaaa tctcattatg attaaaaaac tctctctcat cgctgttttt gctcttaccg
    26881 cctgcggttg gcactttaaa aacaatgagg tattacctga atcgttcaga acattaacct
    26941 ttgaaagtgc agatcaacac agcgaaatgt ctcgaatttt acgcaatcag ttacaactaa
    27001 gcgatgtaaa attagtgcct agcacggcta atgtggcaaa attacgtttg gtttcaacct
    27061 caacagacag caaagtggtt tctgtgttta aacaagctcg tgaagcagaa aaaatcttaa
    27121 cgctaaacgt tgatgcgatt gccgatattc caaataaagg gcaataccct ttagaagttt
    27181 cggttcatcg tactttcttt gatgactctc gtgcagcttt ggcaaagtct gctgaaaaag
    27241 agatgattat gaaagatatg tatgaacaag cctctcgcca attaattatc aaaatggcgg
    27301 gcttaaaaaa agaactctca aaataatcat tgtaggggcg aaatatgttt cgcccattgt
    27361 ttatctgtat gcaaaaaatc tttccggaaa cacttcaatt ttcattaaac aaaggactac
    27421 agccctttta tctattaaca gggaatgatc tgctattggt gaatgaaacc aaagatgcca
    27481 ttattcacac cgcccgtctc aacggttttg atgaaaaaaa agaagttgaa atcaaaaacg
    27541 atactaactg ggaagaactt ttcgaagcag ctcaatcaat gggattgttt tttgcacgcc
    27601 aaattatggt gctgaatttt ccggattccc ccacagcgac acagtttaaa aaactggctg
    27661 agctttgtgc ctttattcat agtgatttgc tctttatcct gcacttacct aaattttcca
    27721 aaacaatgga aaaacaggct tggttcacgc aaattgagct aaatctcgtg caaattaact
    27781 gccaaacacc ggaacttagc aaacttccac aatgggtcag tcatcgagca aaagcgatga
    27841 atttgcaaat tgaaaatgaa gcaaaccaac tcctttgtta tagctacgaa ggcaatctac
    27901 tggcgcttaa acaggcatta caactgctac aactcaatgc aactgatgga aaaatcacgc
    27961 tgacaaaagc ccaagaagtg gttgaacact ccgcccaatt tactccattt caatggattg
    28021 atgcattatt tgaaggaaaa atccaaagag ctcaacgcat tttgtaccat ctgcaaaatg
    28081 aagaagtaca agcggtcgtt ttattaagaa ttgtgcaaaa agagttgatg attttactcg
    28141 aaatcactcg caacccaacc gcagtcgttt ccagcgaaca atcacttttt atcggtaatt
    28201 tacgaacaga atttgaccgc ttgaaaattt ggcaaaaccg cagacctttt tacaaccaga
    28261 tcactcaacg actgacttac caaaaacttt acgccctaat ccaacaactg gctgaattag
    28321 agcgtaaggt gaaacaagaa tttagcgatg atatttggca agaattagag cgtttttccg
    28381 ctcaatttac ctaactaata cttttctaaa tcttcgctaa acaactccgc ttcttcactg
    28441 gcgatagagt gcttttctgc cgcccaatcg cctaaatcaa taatttgaca acgctcgcta
    28501 caaaacgggc ggtatttact ttctttactc cacaccactt ctttttcaca agtcgggcag
    28561 tttacaatag tttctggcat tctttttcct tttttgctaa tgctaagtat ttataatgta
    28621 aggcttgaac ctgctcttta attaaaagca agccctgttc aaatggcaag ttattttcaa
    28681 tcacatcatc agcataactt aatctttctt cacggctgac ttgggctgcc ataatatttt
    28741 taatggtttc aattttactg ttatcacgtt tggttgctct ttccagttgg atttcaggag
    28801 aaacatcaac aaccaatata cgatcacaat attcggtgag cttattttca attaaaagcg
    28861 gcacgaccca aatcacataa caagcggtcg attcttgcaa tttttttacc atttcttctc
    28921 gaatggcggg gtgaagtaaa ttattgagcc acgttttttc ctcttcattg ttaaacacaa
    28981 tttggcgaag tttagtgcgt tctaatgctt tttcagcggt taagatctct tcaccaaaat
    29041 gctctacaat cttggctaaa agcggtgtac ccggttcaac aacttggcga gccaccacat
    29101 cagcatcaat tacctctacc ccaagagctg aaaataaatc agacactgtg gttttgccac
    29161 tgcctattcc accggttaag cctacaacgt aagtcataaa aattgattcc tcaaaataaa
    29221 aacaaaccaa ttctagtaat taatccctct gctttcaagc attagcacca gcaacatcgc
    29281 tgtgctaata aacggaataa acgccaattt tgctaatttc tgctttttca ttagccaata
    29341 agccaagtaa taaccaatcc ctagcaggct ggcggttaat atcaataaga gcattttttc
    29401 gagccgaaag agtggggata acgcaaccaa taacaacata tcgcccgagc caaatgcctc
    29461 ctttttaaac ataagctgtg aaaccaagcc aatcaacagg caaaaagcaa gggtaaaggt
    29521 aaaagtttgg tgttggaaat cggctaaact ctcccataac accaacaaaa atataagcac
    29581 gaggtagcga atatccgtta ggtaatagca catatccagc agtgataaaa aacagagcag
    29641 aataaaaatc gtgccgataa tagggctttc cgagcagagc ataatcaatg gaaaaaggag
    29701 aaaaaataga tttgccgatc ggggttgttt cggttgtagc accgattgag ccaaaaactg
    29761 ttgctcggtt aagccaatat tcactaatga agttgtttct tggtaaattt ggcgattgac
    29821 tcgaatggca aatcgaggga tctccgcttt gagccaaacc gcaatgaaga gggaaaacag
    29881 tattaacatc gctactccac caaagccccc atatcaaaaa tcggtaaata cagcccaaca
    29941 ataatagtgc cgacaatcac gcccattatc aacattagca tcggttctaa tagctgagac
    30001 aaaatatcaa tttggtaatc taatttttgt tggtacatct cactaatatg ctcacacatt
    30061 ttcgccacat tgccactttt ctccccaacc tcaagcattt gtgccatctc agcaccaaac
    30121 acattcgggt tgatcccttg tgaaaatttg tagccttgtt gtaacaaatt aagcatagtg
    30181 ccaatttctt gctgtaaaat cgggtcatca tttttctcgg ataaaaacga cctcaaggca
    30241 acatcaagcg gaatatgagc attcagcatt aaggcaatat tttggctgaa aaagataatg
    30301 cgagattgct caataattcg gctaaacagt ggcattttag cccatagagc cattttccac
    30361 tttgcgacta acctgctctt ttttgcaaaa aacaggtaaa aaatgaccgc tagtagtaac
    30421 gtgccaagta atactgtgcc ttgctcaatc aagcattcgg aaagggtaaa taaaatatcg
    30481 gtaatgaggg gtaaagtttt gtcttttgag ccataaagtt gggcaaactg cggtacaatc
    30541 aaaaccaata gcccgataga taaagcgagc gaaatcagca acacaatcac cgggtaaaac
    30601 aggattttct tcactttttt cgccaatttt tccaatttcg ctcggctttc agctaaatta
    30661 gtcagcataa tgcccagttt tccgctttgt tcgcccattt tgattagctg aatttcctga
    30721 ttggtcacaa attcacccaa tttctccaac gattgtgaaa aagcatagcc actttcaata
    30781 tgctgaatca gctcattaag ccatagataa aggcggatat ttcggcagtt ttgcaacaac
    30841 atagataatg cctgttttaa cggcatggca gaattaacca acaatgcaag ctgtgatagc
    30901 aagctattca cctcttcttt tttcgggttt gctgaaagca caaaatttcg gctaatccga
    30961 atatgctgat aaccttgtgc cagcaaaagg ttttccaaca tgtctcgatt cgttgccaac
    31021 cgcttgcccc gctgtttttg cccaaatcgg ctctgggctt gccaatgaaa ttcatagatt
    31081 ttcgtcattg cctaagcaat ttctcttttt gtggttcaaa atgaatatct gaggcgttgt
    31141 gttgcaaaca gaacttaaat aaattctcta aaatttggat aatcggatcg tttctatcca
    31201 cttgttccga atgagccaat gaatacgccg tattctcctc ataaatctca ctctgccgac
    31261 tactctgctc aggcgaaagg gcattgagta aaaacttcaa ttcatcggag gaaatcaaaa
    31321 caggctcaat ctgtttgtgg tagataaacg caaaaatctc acaggcattg agattttgca
    31381 tatcatcaat ccctagccaa agctgttctt ctgtttcttg cagcggaatg gctaaatatc
    31441 gcaataaaat ctgcttttca ttgcaatttt tctgccactg ctctgctgaa atctcgatga
    31501 acctttggct gtttaaatcc gttaccgaat actccatttt gatttcctca cctcaaatta
    31561 gccgtttggt gtaacagttg tgctacagaa attagctgga aataggctcg tatcattgcc
    31621 actacaactt gccgtccaac taatcgtatt atttgagaac gacggtgtta aggtatagcc
    31681 ataaccattc agagagcctt tgccggttac cgtaatcacg ccttttgtta ccgcaattga
    31741 gtttaggtat ttagtggtat tactactggt tttattcgcc tgaatcccat tcgtgccacc
    31801 ggagcagtta tccaacccgc cactgttgta aatacagact tcaacgtctg atttatacga
    31861 agcagatgct tgtaataact ccgataacgc cgccttttgg gtgtatgagt tataggaagg
    31921 gatcgcaata gtggcaagaa tggcaataat cgcaatcacg atcatcaact caatgagggt
    31981 aaatgcccga tgcagttggc gaatgccaag cggtctaaaa tgtacaattt tttgcatata
    32041 aattccttat aaattgcacc aacaaattgt ggcgaatcaa gtgtgcaaat tttttgcctg
    32101 ctttgcgacc ggcaaatttt aatttttcga cgcagatcga aaaaataaaa tttttatctg
    32161 tttatccata cagttttttg ttagaatttg ccaaatttac tcaattagga atcaagaatg
    32221 gcaaaagcac ctaaaaccgc ttatgtttgt aacgattgcg gggctgaata ttcccgttgg
    32281 atggggcaat gtaaggaatg taaggcttgg aataccatca gtgaagtgcg gttgatttcc
    32341 agcaaagaag cggcgaaatc tgaccgcttt agcggctatg ccggtgaaac gtcaggcaaa
    32401 gtacaaacgc tgtctgacat cagcttgcag gaagtgcctc gctttagcag tggctttaac
    32461 gagctggatc gtgtactcgg tggcggcgta gtgccggggt cagcaattct gatcggcggt
    32521 cacccggggg cggggaaaag tacgttatta ctgcaagtaa tgtgcggttt agcccaacat
    32581 ctgccaacgc tgtatgtcac cggtgaagag tcgttacaac aagttgcaat gcgggcaaat
    32641 cacttaggct taccaacggc aaatctgaaa atgttgtctg aaacctctgt cgagcatatt
    32701 tgcaatattg cagatcaaga aaagcccaaa attatggtga ttgactcgat tcaggtgatg
    32761 cacttgtcgg atattcagtc ctcacccggc agtgtttcac aagtgcgtga atgtgcatct
    32821 ttccttaccc gctacgccaa aacccgccaa gtggcgatta ttatggttgg gcacgtcact
    32881 aaagatggca cgcttgccgg cccgaaagtg ttggaacacg ccatcgatgc ttccttgctg
    32941 ttagaaggcg aatccgattc ccgtttccgt actttgcgta gccagaaaaa tcgttttggt
    33001 gccgtcaatg aattgggcgt atttgcgatg accgaacaag ggctaaaaga ggttaaaaac
    33061 ccatctgcga tttttttaag ccgtagcgaa gagcaaacgc cgggcagttc ggtgatggtg
    33121 ttatgggaag gaacacgccc attgttggtt gaaattcaag ccttagtcga tcactcaatg
    33181 ctggcaaacc ctcgccgtgt ggcggttggc ttagatcata accgtttatc gctattactt
    33241 gcggtgctac accgacacgg tggcttgcag atgtcggatc aagatgtgtt tgtaaatgtg
    33301 gtcggtgggg tgaaagtcac ggaaaccagt gccgatttag ccttaatttt ggcgttaatt
    33361 tccagcttcc gtaaccgtcc attgccgcaa gatttggtga tttttgggga agtcggctta
    33421 gcaggggaaa tccgcccggt taccagtggg caagagcgga tcagcgaagc cgcaaaacac
    33481 ggctttaaac gagcgataat tcctcacggc aatgtcccga aaaaggcaat taaagatatg
    33541 caggtgttca ccgtgaaaaa actcagtgat gcgttagata ttctgtataa tctctaaaat
    33601 caagaaccgc ttgtaagtct gtcttacaag cggtctgttt taggtaaaaa tttacgaatg
    33661 tgcggcagtt ggtctattgc ctttggaaat atagtataaa ttaatacaca ccaccaacgc
    33721 atttaagccg gtgaccggaa tcgagccaat cagcaaacca taaacaacga aacaggcaca
    33781 acccagcgaa ttcaccaccc gcagtttaat cacatctttt aataaaaaag aacaggcaac
    33841 taaaatcatc gccacatagc cgagtatttc aacatagtcc atttagaacc tcctcaaata
    33901 aaataaactg ccgatatttt aaaggctgtt tttttatttg tatctagctc taaaaatagc
    33961 aaaggtttgc gttcacattt ttacttttaa ccgcttagtt attaaacaga ttttcgttct
    34021 aaacacataa aacggcaacg gaaagggtta ttttcatcaa tttctgacca cttttcttct
    34081 actaacgccc aattttgttc atcaaattca aagaacgtat ccccttcaat gtctgcttga
    34141 atctcagtta aataaagtct ttctgctagt ggtaaggctt gcttaaacag ttccccccca
    34201 ccaataatca ttatttcagc tgtatttgca aaatttttcg ctaattcaac cgcttgttcg
    34261 agcgattgta ccgcataagc cccttccacc gtaaaacctg aacgggaaag aataatatta
    34321 gggcgttttg gcaataacct gccgatagat tcataggttt tccgccccat aatcaccggt
    34381 ttgccgagcg tgttttggcg aaaccaggct aaatctaccg gcaaatgcca cggcatttgg
    34441 ttatctttgc caatcacctt gttgattgtt cgggcaacaa taatgctgat tttcattttt
    34501 tctcctgtta tgcaaattgg tttgatttta tatcaaatta agcgtgattt tacaccctaa
    34561 aagtgatact tttggcaaca ttatttttag ttacaagtga acagattatg tcaaaaacta
    34621 tcgtgataaa atttggcacc agcactctca cacacggcac taaaagttta agccgtcctt
    34681 atatgttaga gctggtaaag ctcaattaca ccaacagcat cgtgtgattg tggtgacatc
    34741 cggagctgcc gctgccggtc gggactattt aggacaccct gaactcccta aaaccttagc
    34801 ctctaagcaa atgttagcag cggtcgggca aagtcagctc attcgagtgt gggaaaattt
    34861 attcgatatt tataatatcc atatcgggca gatgctctta acccgagccg atctggacga
    34921 tcgtgaacgt ttcctcaatg cccgtgatac ccttgatgcc ctgcttgcac aaaaaatcat
    34981 tccggtgatt aatgaaaacg atgccgttgc taccgaagaa tttaaagtag gcgataacga
    35041 taatttatcg gctttagttg cgattttggc tcaagcagat cagctctatt tattaaccga
    35101 ccaagaaggc ttattcagtg ccgatcctcg ctcaaaccct gatgctaaat tgctctcggt
    35161 ggtggaaaaa atcacgcctg aaatccgcca aattgccggt ggtagttcaa ccggtttagg
    35221 cacgggcgga atgagtacca aaattactgc tgctgatatt gctacccgct ccggtgtgga
    35281 aaccatcatc gcctccggtt ctcgcccaaa tgtgttagtt gatttagcaa acgggcaaaa
    35341 catcggcagt aaatttttag tccaatctga ccgcttggaa ggcagaaaac gctggttatt
    35401 tgctgctccg caagtgggtg aaattgtggt agatttcggg gcggaacaag ccttgcttca
    35461 acataaatca ttactgccgg tgggagtttc tgccatcaaa ggcgaattta ctcgaggtga
    35521 agtaatcaaa attttcagcc aagacggcaa agccttagca ctcggcattg cccgttatag
    35581 cagtgaggca ttgctgcgaa ttaaaggtaa gaaatcaaaa gaaattgaat cgctccttgg
    35641 ttatgaattt ggttctgttg cagtgcatag tgacgaaatg gtggtgtatt aatggaattg
    35701 ggaattgatg ttttagcaat cttgtttgcc gttgcgaccc ttgccggctt tatcgatgcc
    35761 atcgctggcg gcggtggctt actcactatt cctgcattaa tggcagcagg gcttcctccg
    35821 gcaatggcac ttggcacgaa taaattacag gcgtgtggcg gatcactctc ttcctcactc
    35881 tattttattc gcaaaaaagc tgttaattta aaacaaattt ggttgctaat tctatgcact
    35941 tttttaggct ctgccgccgg tactattttt gtgcaaatga tcaatgtcga tttcttaaaa
    36001 acattgttgc ctttcttaat tctgattatc ggcatctatt ttttggtcag ccctaaaatt
    36061 ggcgatgaag atcgtaaaca acgcattagc tttaccctat ttgcctttac cgccgctgtc
    36121 gctctcggtt tttatgatgg aatgttcgga ccggcaaccg gctcttttat gactctggca
    36181 tttattttgc tgctcggctt caacctctct aaagccgtag cacacgccaa agtgattaat
    36241 tttacctcca actttgcctc attaattttc tttacgcttg gtggtgcggt aatgtggaaa
    36301 atcggcttaa ttatgatggt cgggcaattt attggcggca cactaggtgc gaaaatggtg
    36361 atgacaaaag gcaaaaaatt aatccgtccg atgctagtga ctatgtcttt tatgatggtc
    36421 ggcaaaatgc tctacgagca aggaattttt aattaacaat gaataaagca aaacgtattg
    36481 agattttaac ccgtttacgc aatgaaaatc caaaaccgac aactgaactc aattacagta
    36541 atccgtttga attattaatt gccgtgattc tatccgccca agcgaccgat aaaggcgtaa
    36601 ataaagccac cgataagctc ttccctgtcg ccaacacgcc acaagcgatt ttagatttag
    36661 gcgtagatgg gctaaaagag tacatcaaaa ctatcggctt atttaacagc aaggcagaaa
    36721 atattatcaa gacctgccga gatctgatcg aaaaacataa cggcgaagtg cctgaagaca
    36781 gagatgccct cgaagccctt gccggtgttg gcagaaaaac cgccaatgtg gtgatgaata
    36841 ccgcattcgg acaccctacg attgcagtcg atacccatat tttcagggtg tcgaaccgca
    36901 cgaacttcgc tcccggcaaa aatgtagttc aggtagaaga aaaactgctc aaagttgtgc
    36961 cggacgaatt taaagtcgat gttcaccatt ggttgatttt acacggacgc tacacttgca
    37021 ttgcccgtaa gcctcgttgt ggttcttgta ttattgaaga tttatgtgag tttaaagaga
    37081 agacggaata acattatctg gtggtattta atgaataact cccctcttta gaaaagtagg
    37141 ggctggggga gatttgatac tatttcaaca tttcgtatga gcgtatagaa attctgccaa
    37201 atcttcccta tcccctcttt gctaaagagg ggaactagat cgagcgtaaa atataacaag
    37261 cggtcatttt tttgagattt tttgcaaatg gatatttatt tagtcggtgg agcagtgcgg
    37321 gatcaacttc ttgggctgcc agtgaaagat agagattttt tagtggttgg cgcaactgcc
    37381 gaacaacttt tagcccaagg atttcaacaa gttggggcag attttccggt gtttttacac
    37441 cctgaaacca aagaagaata tgcccttgct cgccaagaac gcaagaacgg caatggttac
    37501 aacggcttta tttgcgattt ctccccaaac gtcacgcttg agcaagattt gatccgccga
    37561 gatttaacca ttaacgccat cgcccaagat gaaaacagca cactttttga tccttatggc
    37621 ggtgtgcaag atttagaaaa tcggctactc cgccacgttt ctcctgcatt cagcgaagat
    37681 ccgctacggg tgctacgggt tgcccgcttt gccgcccgtt ttcattcatt tggttttacc
    37741 attgctcctg aaaccctgaa attaatgcga gagatggtac aaagcggtga attgaaacat
    37801 ttaaccgctg aacgtgtgtg gttggaaaca cagaaagcct ttgaaaccga taatccgcac
    37861 gtttattttg acattttacg cttaattggg gcattaaaag tgctattccc tgaactggat
    37921 cgtctgtttg gcgtaccaca accacctcaa caccaccctg aaattgactc cggcaaacac
    37981 actctaatgg tgctacaaca ggcaaaaaga cttgcaaaaa aagcggaaaa tccgaccgct
    38041 ttactctttg cagccctttg ccacgactta ggcaaaggct taacgcctgc tgatattttg
    38101 cctcatcact acggacacga agtcaaaggc attcaaccaa cccgagagtt agccaatcgg
    38161 ttaaaaatcc caactgagat taaagatttt gcccttttag tgaccgaata ccacacgcat
    38221 tgccataaaa tggcggaact tcgccctgaa acggtaataa aactgtttaa cagcctagat
    38281 gtgtggcgta aaccaaaccg cttttttgat ttcctgttcg cctgtgaagc ggatgctaga
    38341 gggcgattag gttttgaaca gcggaattac ccccaacctg aattggcgaa aaaatattac
    38401 caagccgcaa tcgaaattga tgtgcagcaa gttattcaag acggttttga aaaacaagca
    38461 attcgggaag agctgaataa acgcagagtg tatgcggtaa gaagcatcaa gctagatagc
    38521 gacctaataa accattaggt tatccgccac tttctcaaaa tcaattccca aagttcaagt
    38581 tggttaaatg tatggattct ttaaaaggtg gcgttatttt agtgaattta taatgttcct
    38641 atgctcttga ttgttgtagc cacaatttca gggtaaagat cagcaataac ccaatgccga
    38701 aattggcaat agtgaagccg gctaacccaa taaaagataa aggttcaaat tgataaaatt
    38761 caatcgcacc tcttgccatt gcagctaaac caaatgaaaa tgcccagaaa cccatcgaga
    38821 agccattttc agctatccat ttgaggtttc gtaaaataaa acagaattgt aagaatccat
    38881 atccccataa catttttgcc aacaagtcta attctccacc catagaaaaa taggcagaag
    38941 cgcccacaaa tgccggagca agaacaattc ctaaagatgg tctagcaatc ggaaaaagcg
    39001 gactaagccg aattctttgt aacatagtgg gttcatatac taaccaggaa attatgccaa
    39061 tgcctaaaaa taaagaggca atttcactgt agccaattaa gcttaatgaa cttgccatag
    39121 tgaagctccc tgcaattggc ggtaggtaat gtaccgggtg tagcgtttcc ggcaaaaatt
    39181 gtgtgccttt ccataatcca ccaataataa aaaccaagaa aattaaatgc acaatgacgc
    39241 ctaaaaaaat caatagttcg cctaaataaa aatcccatac atagagcaca tcaccgctta
    39301 tcatggttgt cattgtcact aaggaaaata aacaacattg cactgggtgc ataaattctg
    39361 ctttaatctg ctcaaaacaa aaaaacacac gatacacata acagcctaat aaggctaccc
    39421 aaagtatgac tgacgttcca gcaatgatat taccaattgt tgtagcataa ggagtgatag
    39481 gcgttgcatt taaccaggca aggcctaacg cccccagccc taatggcatt gcaaaaaagc
    39541 ctgtagttat tggaaaaggt ttagattcac tcataaattt atcctcatat tgaaataatg
    39601 cataactgta ttcggcttta taacagtcat atgtaaaaaa ttttattttt ctaaccgctt
    39661 gtaataagaa aaacctaaag cacttgcttt aggttcctac tactattcat actcttctag
    39721 ccagatagtt aaaatcgctt caagaatatg ttcatttgat gcttccggat catcatcgaa
    39781 atcttccatt tcacaaatcc atttatgcat atctgtaaaa cgaaccgtgg tcgggtctaa
    39841 atccgggttc atatcataca gattttcagc gatcattcgg gtatctgtcc atttcattag
    39901 tgcgctgcct catttgcgtg atttaaatta tattttggaa tttcaacaac caaatcttct
    39961 tcaccaacaa tacattggca acttaaacgg ctgtctattt ccaatcccca agctttatcc
    40021 agcatatctt cttcttgatc tgaagtatca tttaacgaat caaaaccctc acgaattacc
    40081 acgtggcaag tggtacaagc acaagaacag tcgcaagcgt gatgaatttc aacgcctgct
    40141 tcgtgtgcta cttctaataa attatctccg gctttagctt caacaaccat accttccggg
    40201 caaaattctt cgtgtggaag aaaaacaatt tttggcattt taaagtttcc ttattactaa
    40261 tcccattcaa tattatccag atgttcgccg atgaagttta aaatctcttc ctcattttca
    40321 accagataaa caataatctt ctttaattcc cttgctttct ctttactttt ctcggaaagc
    40381 ggataggtaa cataataaca tcctacgtcc ggatcttctt ctaataactc taataggctt
    40441 ggacgataaa tactaagata agcccgtaaa aatgcagccc agttataacc gttcatatag
    40501 gcaacatcat attcagtatt aatccgctcg cccaactcaa aaatgcggtt atcctctaaa
    40561 tcgaattcaa ccgcaacggt tctgaattgt tctgaaatct cggtttttac atagttagac
    40621 atactgcctc cttatgctaa atcgtccatt tttttaccgg ctaatgcttt ttgaattgat
    40681 aaattcatac gccgtgctgc aaattcttgc gtagctaaat ccacctcttt gataccttgc
    40741 tgaattgcaa agcgatcagt accttctttt aattcgatta aacgggctaa ttgtgcttca
    40801 atcgcttgga actcctccac actcaacact gcagagccat cttctttcaa tgccgttacc
    40861 acgctgtcaa tcactcggtc ggcttctact cgctgttcaa ctaactggcg ggcttccata
    40921 tcttctttgg catttgccat tgaggattta atcatctgcg taacttcttc atcggttaag
    40981 ccataagacg gtttgatttg aattgaagcc tgcactttgg tcgatttttc cattgcagtc
    41041 acgtttaata aaccgtctgc atcaacttgg taagttaccc gaacctgtgc aacccctgct
    41101 gccattgccg gaatgccacg caaagtgatt ttaccgagag agcgacaatc ttcgactaat
    41161 tcacgctcac cttgtaaaac gtggattgtc atcgccgttt gaccgtcttt accggtagta
    41221 aactcttgag ctcgagctac tgggatagta gtgttgcgtg gaataatttt ctccaccaaa
    41281 ccacccatcg tttcaatacc aagcgaaagt ggcacaacat caagcaacaa catatcggaa
    41341 tccggtttgt ttccaactaa aatatccgcc tgcactgccg caccaatggc aacaacttta
    41401 tccgggtcga tagaggttaa tggcgtttta ccgaaaaact cgccgacttg ttcacgcaca
    41461 aacggtacac gggtcgaacc gcctaccatt accacttcac gaacctcttc tgcttcaact
    41521 ccggcatctt tcaaggctcg gcgacaagtc atcagcgagc gttttacaag cggttgaatt
    41581 agctcattaa attgcgaacg gcttactacg cctatccaat ttgcaaattt cacttcaaat
    41641 tcaaccgctt gcgaaagtgc gactttggtt tgagttgcca aagttaataa ttcacgctgc
    41701 tcgcttgccg tttgaggttt gtaatttgcc tgttcagcaa tccattccgc taataggtgg
    41761 tcgaaatcat cgccaccgag tgcggtatca cctgcggttg ctaatacttc aaacacacct
    41821 ttgcgtaaac gcaaaataga taaatcgaaa gtaccgccac ctaaatcgta aacggcaatc
    41881 acaccttctt gtccgctttc taaaccataa gcaatcgctg ctgccgttgg ttcattgagc
    41941 aaacgtaata catttaaacc cgcaagacgt gcggcatctt tggtactttg gcgttgtgcg
    42001 tcatcaaaat aagccggtac ggtaattacc acgcctgaaa gctcaccggc taaacgcttt
    42061 tcggcaaatt gtgccaagtg gcttaaaata tccgccgaaa cttcaatcgg gcttttatta
    42121 ccttgcgctg tttggattaa aggtaaaccg ttttcgctcg cgacaaattg ataaggtaaa
    42181 ttttgataac gttgctgaac atctgccaga gaacgcccaa tgagacgttt agctgaaatg
    42241 acggtatttt tcgggtcaag tgcagctttg gcaaaggctt caatcccaac tgttttttct
    42301 gtttccgcat aatgcactac tgacggcact aatgctcttt cttgttcatc aagcaatacc
    42361 tgagtctgcc cgctacgcac agttgccact aatgaattgg ttgtacctag atcaattcct
    42421 accgctaatt tatgttgatg tggtgcagcg gtttgaccgg gttcagaaat ttgtaataat
    42481 gccatttatt tatctcttta tctttctaac ttagttctct attgtattaa atggagtgaa
    42541 atatattgtt taagagaagc tggag
//
LOCUS       DANLYI010000003        40483 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.003, whole genome shotgun sequence.
ACCESSION   DANLYI010000003 DANLYI010000000
VERSION     DANLYI010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 40483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40483
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.003"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            193..1830
                     /gene="pyrG"
                     /locus_tag="PXD81_000106"
     CDS             193..1830
                     /gene="pyrG"
                     /locus_tag="PXD81_000106"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005595836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase (glutamine hydrolyzing)"
                     /protein_id="HDL5536181.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLASILEARGLNVTIMKLDPY
                     INVDPGTMSPTQHGEVFVTEDGAETDLDLGHYERFIRSKMSKANNFTSGKIYSEVLRK
                     ERRGDYLGATIQVIPHITNEIKARVLEGGKGRDVAIVEVGGTVGDIESLPFLEALRQL
                     AVDIGREKTLFMHLTLVPYIPTAGEVKTKPTQHSVKELLSIGIQPDVLICRSDRAIPE
                     NEKKKIALFCNVPERAVISLKDVDSIYRIPELLKSQGLDSFVCDRFRLDCKEADLSEW
                     EQVLYCQANPTGEVTIGMVGKYVELPDAYKSVNEALKHAGLTHRLTVNIKYIDSQDIE
                     TKGTELLAGLDGILVPGGFGYRGVEGKIRTAQYARENKIPYLGICLGMQIALIEYARN
                     VAGLTEANSSEFDKDCSQPVIGLITEWQDAEGNVETRSDESDLGGTMRLGSQQCHLIE
                     GSKARELYGAETIVERHRHRYEVNNKLLPQIEAAGLKVSGLSADRKLVEIIEVPNHPW
                     FIAAQFHPEFTSTPRDGHPLFAGFVKAAKDHQESRKA"
     gene            complement(1998..2594)
                     /gene="nfuA"
                     /locus_tag="PXD81_000107"
     CDS             complement(1998..2594)
                     /gene="nfuA"
                     /locus_tag="PXD81_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010944401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HDL5536182.1"
                     /translation="MEEQTIHISISDAAQAHFARLLEQQEEGTNIRIFVVNPGTPGAE
                     CGVSYCPPNAVEETDTEFKFNRFSAFVDDISLPFLDEAEIDYVTDPMGSQLTLKAPNA
                     KMRKVADDAPFIERLDYVIQTQVNPQLASHGGKVTLIEITEDKYAVLQFGGGCNGCSM
                     VDVTLKEGIEKQLLAMFPDELVGVKDITEHQHGEHSYY"
     gene            3041..4399
                     /locus_tag="PXD81_000108"
     CDS             3041..4399
                     /locus_tag="PXD81_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005595863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)-translocating NADH-quinone reductase
                     subunit A"
                     /protein_id="HDL5536183.1"
                     /translation="MITIKKGLDLPIAGKPEQVIHNGNTVTEVAMLGEEYVGMRPSMK
                     VREGDVVKKGQVLFEDKKNPGVVFTAPASGTVTAINRGEKRVLQSVVIKVEGDEQITF
                     ARYEASQLASLTSEQVKQNLVESGLWTAFRTRPFSKVPALDAEPSSIFVNAMDTNPLA
                     ANPEVIIAEHQQAFFDGLTVLSRLFESKKVIHLCGSADSNILNAAQSRQINNVETNRF
                     KGPHPAGLSGTHIHFIDPVGATKQVWYLNYQDVIAIGKLFTTGELFTDRVVALAGPQV
                     KNPRLVRTRLGANLSQLTANELNAGENRVISGSVLSGAIATGVHDYLGRYALQVSALL
                     EGREKAFFGMIAPYSDKYSITRTTLGHFAKKLFNFTTAVHGSERAMVPTGAYERVMPL
                     DIIPTLLLRDLAAGDTDSAQALGCLELDEEDLALCTFVCSGKNNWGPLLRQALDKIEK
                     DG"
     gene            4402..5637
                     /locus_tag="PXD81_000109"
     CDS             4402..5637
                     /locus_tag="PXD81_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit B"
                     /protein_id="HDL5536184.1"
                     /translation="MGLKNLFEKMEPAFHKGGKYEKWYTLFEAAYTIFYTPGTVTRKD
                     AHVRDAIDSKRMMLIVWLALFPAMFYGMYNVGAQGILAALKMGTLTDLVANNWHYSLA
                     DSLGSVADAGWGTKMLLGATYFLPIYLTVFAVGGFWEVVFAMVRKHEINEGFFVTSIL
                     LALIVPPTLPLWQAALATTFGVVVAKEVFGGVGKNFMNPALAGRAFLFFAYPGQISGD
                     AVWVAADGFSGATALSQWAVGGQGALQHVATGETITWMDAFLGNIPGSIGETSTLMLI
                     IGAAIIVFARIASWRIIAGVMIGMAATATLFNVIGSDTNPLFSMPWHWHLVLGGFAIG
                     MFFMATDPVSAAFTNKGKWAYGILIGFMCVLIRVVNPAYPEGMMLAILFANLFAPIFD
                     YLVVQGNIKRRLARVANNG"
     gene            5630..6403
                     /locus_tag="PXD81_000110"
     CDS             5630..6403
                     /locus_tag="PXD81_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)-translocating NADH-quinone reductase
                     subunit C"
                     /protein_id="HDL5536185.1"
                     /translation="MAKFNKDSVGGTLTVVVLLSLICSLIVAGAAVMLKPTQDIQKQL
                     DKQKNILQAAGLLQKDTNVQETYTKFIEPKIVDLATGDYVEGVTGFDARAAAKDPAQN
                     VVIAPEDDKANIKTRAKYAEVYLVKDESGKTTQVVLPMHGNGLWSIMYGFVAVQPDAN
                     TVNGITYYEQGETAGLGGEIANPNWQKNFVGKKLYNEQNQVALRVGKGASADKDHGVD
                     GLSGATLTSNGVDGSFKYWFGQNGFGPYLAKFKAAAGAN"
     gene            6403..7029
                     /locus_tag="PXD81_000111"
     CDS             6403..7029
                     /locus_tag="PXD81_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005717817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit D"
                     /protein_id="HDL5536186.1"
                     /translation="MADNNNLKKLLLSPIVDNNPIALQILGICSALAVTTQLQTAVVM
                     AIAVSLVTAFSSMFISMIRNYIPNSIRIIVQMAIIASLVILVDQILRAYAYDLSKQLS
                     VFVGLIITNCIVMGRAEAFAMKSGPVESFVDGIGNGLGYGAMLIIVAFLRELIGSGKI
                     FGITILETVQNGGWYQANGLFLLAPSAFFIIGFVIWAIRTWKPEQVEK"
     gene            7031..7627
                     /gene="nqrE"
                     /locus_tag="PXD81_000112"
     CDS             7031..7627
                     /gene="nqrE"
                     /locus_tag="PXD81_000112"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005529392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit E"
                     /protein_id="HDL5536187.1"
                     /translation="MEHLLSIFVKSIFIENMALSFFLGMCTFLAVSKKVSTAFGLGIA
                     VIVVLGIAVPANQLVYTHVLKEGALVEGVDLSFLNFITFIGVIAALVQILEMILDKFF
                     PALYSALGIFLPLITVNCAIFGGVSFMVQREYNFVESVVYGVGAGTGWMLAIVALAGL
                     TEKMKYSDVPAGLRGLGITFITVGLMALGFMSFSGIQL"
     gene            7638..8867
                     /gene="nqrF"
                     /locus_tag="PXD81_000113"
     CDS             7638..8867
                     /gene="nqrF"
                     /locus_tag="PXD81_000113"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit F"
                     /protein_id="HDL5536188.1"
                     /translation="MDSNFIFGIIAFTALVLVLAVIILFAKSKLVDSGDITISINNDP
                     EKGITLPAGGKLLGALASKGIFVSSACGGGGSCGQCKVQVKSGGGEILPTELSHISKK
                     EAKEGWRLACQVNVKSSMDVELPEEIFGVKKWECTVISNDNKATFIKELKLQIPEGEE
                     VPFRAGGYIQIEAEPHTVHYKDFDIPKEYHEDWDKFDLWRYTSKVDEHIIRAYSMASY
                     PEEKGIIMLNVRIATPPPRNPDVPPGQMSSYIWSLKPGDKVTISGPFGEFFAKDTDAE
                     MVFIGGGAGMAPMRSHIFDQLKRLKSKRKMSFWYGARSEREMFYVEDFDGLQAENDNF
                     KWYVALSDPLPGDREDYFRGFIHNVLYENYLKNHEAPEDCEYYMCGPPVMNAAVIGML
                     KSLGVEDENILLDDFGG"
     gene            8979..9413
                     /locus_tag="PXD81_000114"
     CDS             8979..9413
                     /locus_tag="PXD81_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase substrate binding
                     protein"
                     /protein_id="HDL5536189.1"
                     /translation="MEQLDITVLENAFNSLEKTIVSLENQAWFNRQEAIIQDTLIAGA
                     IQKFEFVYELSIKMLKRQLKNIESNDTDIDSTEFRDILRMAAQYGLIESPEVWLDYRK
                     MRNITSHTYDQNKAQQVYNGISNFLESTYFLVKQLQKRNSYD"
     gene            9406..9699
                     /locus_tag="PXD81_000115"
     CDS             9406..9699
                     /locus_tag="PXD81_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HDL5536190.1"
                     /translation="MIDLTESQRQQVLDIFKKYLPDTPVWVFGSRIKGTAKAYSDLDL
                     ALITKQPLTIRQQTELELAFTDSDLPFTVDLIDWASCNESFKQIILQRYEVLI"
     gene            9748..10782
                     /locus_tag="PXD81_000116"
     CDS             9748..10782
                     /locus_tag="PXD81_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HDL5536191.1"
                     /translation="MKKFFLLLIAFFTLSACEKAPEQITLSGKTMGTTYTVKYIDNGE
                     VENLISPEKMKNELDGLLIEVNNQMSTYQQDSEISRFNQAKQANQAVEISQDFAKVVQ
                     EAIRLNKVTEGALDVTVGPLVNLWGFGPDKRLNKVPSDEQIAERSAYVGIDKILLEMS
                     SKPKLTKSAANLYLDLSSIAKGFGVDKLAEHLETIGVSNYLVEIGGELRGKGKNLQGL
                     DWRIAIEKPVMTQGQAAQITVPLHNLAMATSGNYRNYFEDEKGNRLSHIIDPKQLRPV
                     NHNLASITVFAPTTMTADGLSTGLFVLGAEKALAVAEREKLAIFLIVKNGDKFETQMS
                     SEFSKLIQSQ"
     gene            10795..11040
                     /gene="nqrM"
                     /locus_tag="PXD81_000117"
     CDS             10795..11040
                     /gene="nqrM"
                     /locus_tag="PXD81_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Na+)-NQR maturation NqrM"
                     /protein_id="HDL5536192.1"
                     /translation="METVLLTFGFFVAVIFAMSIGFIVKGKTIKGSCGGITALGMKKM
                     CDCEEPCDNLKSKIADGTADPEEVARFNKEPQFYEVK"
     gene            11042..11458
                     /locus_tag="PXD81_000118"
     CDS             11042..11458
                     /locus_tag="PXD81_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase substrate binding
                     protein"
                     /protein_id="HDL5536193.1"
                     /translation="MNRDIRWKQRFDNYKKSVSYLQAEAEKYADTDIDVIKKGIIQSF
                     EITHELAWKLMQDILKWEGEVDIYGSKTASRLAFNRGLITQGEVWLEMIKSRNITVHT
                     DDETILTEEFNKIIKAYLPLFIEFEQRVETLCQTLD"
     gene            11440..11751
                     /locus_tag="PXD81_000119"
     CDS             11440..11751
                     /locus_tag="PXD81_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HDL5536194.1"
                     /translation="MPNFGLSERATKTITDILAKYPEVEQAIIYGSRAKGNYREGSDV
                     DLTFKGKDLTYDIVKRIWGDLDDSDSPYLFDLSIYHQLTNANFIEHIDRVGKVFYQRE
                     L"
     gene            11862..13025
                     /gene="mnmA"
                     /locus_tag="PXD81_000120"
     CDS             11862..13025
                     /gene="mnmA"
                     /locus_tag="PXD81_000120"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HDL5536195.1"
                     /translation="MTTQLTSKTYDTHFPKLTPEQLAENSKKKVIIGMSGGVDSSVSA
                     FILQQQGYQVEGLFMKNWEEDDDTDYCTAAAELADAQAVADKLGMKLHKINFAAEYWD
                     NVFEYFLAEYKAGRTPNPDILCNKEIKFKAFLEYAAEDLGADYIATGHYVRRTSDDQD
                     AKLLRGLDENKDQSYFLYALNKKQIGQSLFPVGEIEKPIVRAIAEDLGLATAKKKDST
                     GICFIGERKFKDFLARFLPAQPGEIRTVEGKLVGRHDGLMYHTLGQRKGLGIGGVKGL
                     SEDPFYVVEKDLINNVLIVAQGHDNSALLSSGLIAKQLTWVDMQPLRENLRCTVKTRY
                     RQADIACEIQVIDDETIRVMFDEPQIAVTPGQSAVFYLGEVCLGGGIIESQLK"
     gene            13142..14521
                     /gene="argH"
                     /locus_tag="PXD81_000121"
     CDS             13142..14521
                     /gene="argH"
                     /locus_tag="PXD81_000121"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HDL5536196.1"
                     /translation="MALWGGRFTQQADAKFKYFNDSLRFDYRLAIQDIEGSIGWAKAI
                     TSVGILTVAELEQLIAALKEVRAEVESNLTIILKDDAEDIHSWVESKLIEKVGDLGKK
                     LHTGRSRNDQVAVDIKMWCKVQAVALQERIRALQERLVSVAEENQESVMPGYTHLQRA
                     QPVTFAHWCMAYYEMLERDFSRLSDANKRMSTCPLGSGALAGTAYGIDRDLLARDLGF
                     EEATRNSLDSVSDRDHILELLSTASISMMHLSRFAEDLIIFNSGESGFLEMSDRVTSG
                     SSLMPQKKNPDACELIRGKSGRVFGALNGLLTTLKGLPLAYNKDMQEDKEGIFDALET
                     WQACLEIAELVLVDIQVNTERTREAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VYAISKKEPLEALSINEFQQFHHSIGDDVYPILSLESCLEKRCAKGGVNPQRVAEAIA
                     VAKANLASK"
     gene            14689..15687
                     /locus_tag="PXD81_000122"
     CDS             14689..15687
                     /locus_tag="PXD81_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4115 domain-containing protein"
                     /protein_id="HDL5536197.1"
                     /translation="MTESVNSTQAGTALSLGQQLKAAREALNLSITDVAQKTNLKKSH
                     IESLENDIFILQNVAPTFVRGYVRNYVKFLRLPESLISSVNYGEVTIPKEVTKVSSVK
                     PSSNSQGKTLKYLTVLVLLAALGMTLLWWWQGYQKEQENREQFVSSAPVAEVANTATN
                     NDASAQTSVPVQQPIQVSEQPAQVAETVVAAEPALTEKVEPTQAASSPVTQEDVKVVE
                     EVKQADATTAQVEAVNVLQQSQSEQAPTEQPVAVTNDELRIEITGSQSWITVRGAKNK
                     RLAEKLYNNGETLSFNENEQYRLTIGAPANVKVYYKGQEVPLKIDGRVARIHLPLQ"
     gene            15788..16900
                     /gene="ispG"
                     /locus_tag="PXD81_000123"
                     /gene_synonym="gcpE"
     CDS             15788..16900
                     /gene="ispG"
                     /locus_tag="PXD81_000123"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HDL5536198.1"
                     /translation="MIHQSPIKRRVSKKIYVGNVPVGGDAPITVQSMTNTRTTDVEAT
                     VAQIKSLERVGADIVRVSVPTMDAAEAFKAIKQQVSVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRTVVDCARDKNIPIRIGVNAGSLERDLQEKYGEPTPQALLES
                     ALRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARAGSV
                     KSAVGLGLLLAEGIGDTLRVSLAADPVEEVKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EIDVIATVNELEQRLEDIITPMDVSIIGCVVNGPGEALVSDLGVTGSNKMSGFYLDGV
                     RQKERFDNAKLIDQLEAKIRARVAQQHNRIDIEQIG"
     gene            16906..18177
                     /gene="hisS"
                     /locus_tag="PXD81_000124"
     CDS             16906..18177
                     /gene="hisS"
                     /locus_tag="PXD81_000124"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005617650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HDL5536199.1"
                     /translation="MAKTIQAIRGMNDCSPSESPLWQWVEQKVRNVLASYGYSEVRMP
                     IVESTPLFARAIGEVTDVVSKEMYTFWDNDEQLTLRPEGTAGCVRAAIERGWIYNNEQ
                     RLWYMGPMFRHERPQKGRYRQFHQAGVEVFGIANPEIDAELIILTARLWKELGIDQHV
                     SLQLNSIGSLEARANYRSALVKFLENHTALMNEEEKERLVKNPLRILDTKNEALQNVL
                     NDAPKLLDYLDDDSREHFTQLCALLDAMGIRYEVNPKLVRGLDYYNKTVFEWVTSALG
                     SQGTVCGGGRYDGLVEQLGGHATQGVGFAMGLERLVLLVQEVNKEIDLPKTVDVYIVF
                     SGEGATLNAFQLAEQIRSELPHVGVMTHCSGGKFQKQFKRADKVEAQIALVIGESEVA
                     EKQVVIKDLRSGAEQITIAQSELIAELSKRF"
     gene            18187..18807
                     /locus_tag="PXD81_000125"
     CDS             18187..18807
                     /locus_tag="PXD81_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005601670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HDL5536200.1"
                     /translation="MSNEYLNKTEEQQFEDAKNWFKENGTPILVAIILACAATAGWNF
                     WKNHQLETAQKTSASYQSVMESYLQNPSKNQPLVEKFIADNKDSSYAAFAQFEVAKQA
                     VEKGNFSAAKTALQSVLTYSDSTLQSIARFRLAQIDFQLKSYDEALASLAQIQDKAWE
                     LRKQILTGDILAAKGDIPAAKSAYEQAKTTANPQEQTLIDVRLNNL"
     gene            complement(18870..19973)
                     /gene="dnaN"
                     /locus_tag="PXD81_000126"
     CDS             complement(18870..19973)
                     /gene="dnaN"
                     /locus_tag="PXD81_000126"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HDL5536201.1"
                     /translation="MQITITREQLLKPLQQVCGVLSSRPTLPVINNLLLEIEGNRLSL
                     TGTDLEVELTTQAQLLQAVDFAGKFTIPAKKFLDISKSLPDDSVISVQFEEDKAIIKA
                     GRSKFSLATLPASDYPNLMDWKPEVDFSIDQSTLARLIDATQFSMANQDARYFLNGMK
                     FETEGNLLRTVATDGHRLAVCTMVLNQELLTHSVIVPRKAVLELSRLVASNDETVRLE
                     IGSSNIRITMNGIVFTSKLIDGRFPDYRRVLPRNADRILEAETETLKRALMRAAILSN
                     EKFRGVRLTLSNNLLKITANNPEQEEAEEIIDVAYDSPEMEVGFNVSYVLDVLNTLKC
                     QRVRFSLVDSSSSCLIEDCDNSSAEYVIMPMRL"
     gene            complement(19992..21341)
                     /gene="dnaA"
                     /locus_tag="PXD81_000127"
     CDS             complement(19992..21341)
                     /gene="dnaA"
                     /locus_tag="PXD81_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005609583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HDL5536202.1"
                     /translation="MEQIVSSLWSDCLNHLQTKVSPTDYSTWLRPLQASSENGELTLY
                     AQNQFVENWVKDKFLSEITELARFLAKNDNLVVTLKVGIKPTEQPKVSPNLKNESSEF
                     SNTGFKTGLNPNQTFDNFVQGRSNQLAKLVAQQVVDNLGESHCNPLSFYAGTGLGKTH
                     LLHAIGNELVKRTPGTRVLYIHLERFYRDIIKAINSSNNDAEKIKKFYRSLDVLMIDD
                     IQFLANKPKVQEEFLHLLNALFEQRKQIILTSDVLPKNIDKIDTAIKSRLSGGISATI
                     EPPELETRVAILRQKALERGADLQEDAAYLMGQKLRTNVRELEGALNRVIAWQKLTLR
                     PITVDAVRETLQDLFASFDYLITIENIQRIVAEYYGIKVADIKSKSRARSIARPRQMA
                     MALAKELTNHSLPEIGREFGGRDHTTVMHACKTINELRDTESGIQEDYIHLTRKLSS"
     gene            complement(21528..23375)
                     /locus_tag="PXD81_000128"
     CDS             complement(21528..23375)
                     /locus_tag="PXD81_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL5536203.1"
                     /translation="MFEKLFKWFEARVETYPNEMPKTPKSGLIPFIYDATKGMRFYIL
                     LLTVLVAAVGIIEAVLFRFMGDLVDWVNKYSPTELWAEKGWTIIGMFFIALLAVVFVY
                     LASSIRFQSLQGVFPMRLRWNFHRLMLGQSLGFYQDEFAGRVSAKVMQTALSVRDVIM
                     TCADMLVYVLVYFTTSSVILLQLDGWLFVPFILWVISLAATIIYFVPKLAQAAQEQSD
                     ARSLMTGRITDAYANIATVKLFSHGNRESAYAKESMEGFMVTVHKQMRLVTVIETVTN
                     LTSMALIISTAGIGLWLWGSSVVTAGAIATSTALALRIKGLSQWIMWEFARLFENLGT
                     VQDGMITLSKPHNVVDKIDAKPLNVTKGEIKFENVDFAYDPKKPLLKDFDLTIKPGEK
                     VGLVGRSGAGKSTLTNLLLRFYDIQDGSITIDGQNIRDITQESLRSQIGLVTQDTSLL
                     HRSVRENLMYGRPNATEEEMIKAVEQAAASEFIPNLQDAKGRTGFDAHVGERGVKLSG
                     GQRQRIAIARVMLKDAPILLLDEATSALDSEVEVAIQENLTQLMEGKTVVAIAHRLST
                     IMAMDRLIVLDKGEIVEQGTHEELLAQNGVYARLWAHQSGGFLPENTDI"
     gene            complement(23444..23953)
                     /gene="luxS"
                     /locus_tag="PXD81_000129"
     CDS             complement(23444..23953)
                     /gene="luxS"
                     /locus_tag="PXD81_000129"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005706864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HDL5536204.1"
                     /translation="MPLLDSFKVDHTKMNAPAVRVAKTMTTPKGDTITVFDLRFCRPN
                     IDILPVRGIHTMEHLFAGFMRDHLNSEQVEIIDISPMGCRTGFYMSLIGSPSAEEVVK
                     AWTASMEDALHKIPDVSAIPELNEYQCGSYKEHSLKEAHQIARNVLAAGIGINRNEDL
                     ALDEKLLNP"
     gene            complement(23971..26637)
                     /locus_tag="PXD81_000130"
     CDS             complement(23971..26637)
                     /locus_tag="PXD81_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MCE family protein"
                     /protein_id="HDL5536205.1"
                     /translation="MTMEENTHTVVDAQIRQPRKISPFWLLPIVAFIIGALLFFQILK
                     EQGETITIRFSKGDGITAGKTAIRYQGLQIGQVKRVYFVDNLKEVEVQAEINPEAKSI
                     LRKQTKFWLVQPSASLAGVSGLDAIVSGNYITLLPGEGDFVDEFIAEEDPPAITVSDG
                     DLLIRLISDDIGSITVGASVYFRKVPVGNIADYRFTKDQKKIEIDVVINQKYAHLVKK
                     ESRFWNISGISADVNLQGIKINMDSLASVVQGAVAFDSPDNMEIAEQGQKFELYPDLK
                     SAKRGIEINVKLPLTHNLKINETGVFHQNVQIGMLSQLKLPQVKDNGVPQQESGQHKQ
                     IDGVLLIDPSYENLLRSQTKILLKEPKFSLNTEQLTKVGELLRGVYFEVHPGEGEPQS
                     EFVVQNEADYLLSLPNLLSFSLIAPQAYGVDTNQGIYYSDVQIGEIVKRSLELDKVRF
                     DVIIYPPYRNLIGSNSKFVAISNIDMSVGLDGLRVHAGSPSEWLKGGVRLINGKNDGS
                     PKKQYALYKDIESAESGMATLDKKATTTLSADSLSGISEGSLVLYRDFQVGEVLKITP
                     KQQKFEVELFIQPNYRHLLTEKSRFWIEPATSVDVSLKGVNITTAPLMRTLKGAISFD
                     DGGSKGNKTLYASKEKATSGNTYLTLIAKDASKLSEGMDIKYMGLTVGQIEKLELQNA
                     KKQIKATAYIQRKYYPLIAKAGSKFNVISPEISTSGFKNLDAALQNYITVDVGSGQAQ
                     NQFVLADTDTVKTTYANGFPIIVETTNANGIQPEAPVLYRGMQVGIVSRLGLSELGDR
                     VLIYVNIQDKYKHLVRTNTQFWQASGYTMDVSLQGVSMNSGTMSQLLNGGIEFSTPYT
                     KVVKPQAQPNRRFFLQRKLPDEAPAWDQGIAE"
     gene            complement(26720..27160)
                     /gene="fur"
                     /locus_tag="PXD81_000131"
     CDS             complement(26720..27160)
                     /gene="fur"
                     /locus_tag="PXD81_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014990964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HDL5536206.1"
                     /translation="MSEANIKRLKEVGLKVTEPRLTILALMQDIRDEMQHFAAEDIYK
                     ILLEKGSDIGLATVYRVLNQFEEAGILTRHNFDANKAVFELNMNHEHDHIICMDCGKV
                     FEFKDPDMERRQREISEKHGMELTNHSLYLYGKCSNLTNCDEKK"
     gene            complement(27196..27720)
                     /gene="fldA"
                     /locus_tag="PXD81_000132"
     CDS             complement(27196..27720)
                     /gene="fldA"
                     /locus_tag="PXD81_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006717621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HDL5536207.1"
                     /translation="MAVVGLFYGSDTGNTENISKMIQKKLGAELIDIRDIAKSTKEDI
                     EAYDFLMFGIPTWYYGESQADWDDFMPTLKEIDFNGKVVAIFGCGDQEDYAEYFCDAM
                     GTVRDVVEPNGGVIVGHWPTAGYTFEVSQALVDDDTFVGLCIDEDRQPELTTERVNKW
                     CEQLKEEMCLDQLA"
     gene            complement(27726..28001)
                     /gene="ybfE"
                     /locus_tag="PXD81_000133"
     CDS             complement(27726..28001)
                     /gene="ybfE"
                     /locus_tag="PXD81_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HDL5536208.1"
                     /translation="MAKQDSDCITLDLFATVPKVGRPRTNPLDREQQLRINKRNQLKR
                     DKSSGLKRVELKLHSNLVKLLEEQASEQGVSRGQLIETILNNYLTNR"
     gene            28242..29228
                     /locus_tag="PXD81_000134"
     CDS             28242..29228
                     /locus_tag="PXD81_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HDL5536209.1"
                     /translation="MSNIHLAIAADFTLAEKLLEALANQDLGIENISAVELEPFGEEQ
                     NLRFGAKAVAQYALDEVNWAEFSHVLFAGKMAQAEVLAEAVKAGCVILDLYGITALIG
                     DVPVVVPSVNDENVANLRERNIVALANPQISQLALALKPLLNEPLKQVWVTSLLPSAY
                     FGDEKVKELAGQTARLLNGIPFDENAERIAFDVVPANVQGEEKSLPFSKAFELQIAKV
                     LPNLTACTTFHSVQVPVFYGVSQMVSVQSEYQLDANALSEAWKAENWLNFNENSVITA
                     VKNGENEEQNALEISQLLAKSEQEVQFWTVADEQRFSLAFLAVKLLKLVLAY"
     gene            29325..30620
                     /locus_tag="PXD81_000135"
     CDS             29325..30620
                     /locus_tag="PXD81_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005617710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HDL5536210.1"
                     /translation="MLEKLFQLKAKGSNVKTEIIAGITTFFTMVYIVFVNPSILGVAG
                     MDTQVVFVTTCLIAAFGTIAMGLFSNLPIALAPAMGLNAFFAFVVVQKLGYSWQVGMG
                     AIFLGSVGLFLLTVLQVRYWFMSAIPLGLRVGIGAGIGLFIALIGFKNMGLVVPNPAT
                     LVALGDLHDPKVLMGILGFFIIVVLAAKGIHSGVLISIVIVTALALAFDPAVSFNGIM
                     SMPPSLGAVVGQVDIAGAFDVALLGMIFSFMLVNLFDSSGTLIAVTTKAGFADEQGRF
                     PKMKQALLVDSTAAMAGSYMGTSAISTYIESGSGVSVGGRTGLTAVVVGILFLLTIFF
                     SPLAGLVPAYATAGALVFVGILMASSLIEVKWDDLTEATPAFITTAMMPFTYSITEGI
                     AFGFISYCVMKAFTGRWKEINLSVLVVSLLFIAKFVWVG"
     gene            30740..31108
                     /locus_tag="PXD81_000136"
     CDS             30740..31108
                     /locus_tag="PXD81_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/DsrF/TusD sulfur relay family protein"
                     /protein_id="HDL5536211.1"
                     /translation="MSNSVQKLLFIINSAPYGNETFFSALRLALQIQEQHKANTEIKL
                     FLMSDAVTAGLAKQNPAEGYHIQQMLEILTAQGANVKLCKTCTNARGITDLPLADGVE
                     IGTLIELADWTIQADKVLNF"
     gene            31108..32301
                     /locus_tag="PXD81_000137"
     CDS             31108..32301
                     /locus_tag="PXD81_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDL5536212.1"
                     /translation="MQNIDVVVIGAGAAGLFCAAQLGQAGKKVVVLDSGKKVGRKILM
                     SGGGFCNFTNLDVSAGNYLSQNRHFVKSALSRYTQWDFISLVSHYGISYHEKELGQLF
                     CDESSQQIVDLLQAECNKGKVEILLRQAVISVEKVANFFEISTACGKFQSEHLVIATG
                     GLSMPALGASPFGYQLAEQFGIPVVPPRASLVPFTWKESDKYFAELSGIALPITVSAN
                     GKSFSNQMLFTHRGLSGPAILQISNYWELGETVEIDLLPSQSITDILSELRQSSPKLQ
                     LKTVLSRYLPKKLVEVWLEQKLLTDKVIADLSKKDLTLLEQFIHHWHFLPNGTEGYRT
                     AEVTKGGVDTDYISSKTMEAKNTAGLYFIGEVLDVTGWLGGYNFQWAWSSAFACAEGI
                     IEKSK"
     gene            complement(32372..32590)
                     /gene="infA"
                     /locus_tag="PXD81_000138"
     CDS             complement(32372..32590)
                     /gene="infA"
                     /locus_tag="PXD81_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HDL5536213.1"
                     /translation="MAKEDCIEMQGTILETLPNTMFRVELENGHIVTAHISGKMRKNY
                     IRILTGDKVTVEMTPYDLSKARIIFRAR"
     gene            complement(32700..33359)
                     /locus_tag="PXD81_000139"
     CDS             complement(32700..33359)
                     /locus_tag="PXD81_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009875525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HDL5536214.1"
                     /translation="MYISREKITKTLLLAGITLLCCGIVAVILMGVNQFKLGSQLASI
                     NQVSNLSHLLVRQQANIFSFLLVKNAKTDELVDALDSFAKEDFIIDANIYSPNGVLLA
                     QSQNAKDLKVLLDSEQSAKTQQIVEPIFSQQALVGFLRVTFDAEYGQTTKARVEQQFH
                     QLYGEIIILLLTGILLASSINYYFRRKIHHVQISPKPLMSQVKTQTQRFHSRRRWIKR
                     K"
     gene            33445..34308
                     /gene="serB"
                     /locus_tag="PXD81_000140"
     CDS             33445..34308
                     /gene="serB"
                     /locus_tag="PXD81_000140"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase SerB"
                     /protein_id="HDL5536215.1"
                     /translation="MTNTIFIYSKTLNEREIEQFSQQTAAILLKQAVYLDYKIAFFST
                     NLTACELRETAQKINADISDLTIIPTLNQGGLLLMDMDSTAIKIECIDDIAKLAGTGE
                     VVSAITASAMRGELDFEQSLRKRVGTLENASESILQKVRENLPLMDGFEQMVAILKEH
                     GWKLAIASGGFDYFADYLKQKYGLNFAVSNQLEIIDGKLTGKVLGKVVDAQHKADTLQ
                     NLATTFNIPPNQWVAVGDGANDLPMLKTASLGVALHAKPKVQAQADFVVNFGDLTALC
                     LLLNANHLFQH"
     gene            34324..34815
                     /locus_tag="PXD81_000141"
     CDS             34324..34815
                     /locus_tag="PXD81_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HDL5536216.1"
                     /translation="MPSFDIASEITMHEVRNAVENANRVLSTRYDFRGVEAVIELNEK
                     NESIKLTTESDFQLEQLIEILIGSCVKRGIEHSSLDIPTESEHHGKLYSKEIKLKQGI
                     ESDMAKKITKLIKDSKIKVQAQIQGEQVRVTGKSRDDLQAAIQLVKGADLGQPFQFNN
                     FRD"
     gene            34900..35076
                     /locus_tag="PXD81_000142"
     CDS             34900..35076
                     /locus_tag="PXD81_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5363 domain-containing protein"
                     /protein_id="HDL5536217.1"
                     /translation="MMSEQTEKKGWFKKALEKYDAFCDELGVNQGACRGCVPVVKFDP
                     EPEKEQPEKSENKT"
     gene            complement(35124..35420)
                     /locus_tag="PXD81_000143"
     CDS             complement(35124..35420)
                     /locus_tag="PXD81_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5536218.1"
                     /translation="MKKHHIIILTLASASVVACSSNTPVQVVNGDKNIDYSQPKTIYI
                     SETNTQPPALTKQEKIKPSNATALCFNGSYSTDIQNPCKKDGGVKQYFAHHHAN"
     gene            complement(35497..36465)
                     /gene="mdh"
                     /locus_tag="PXD81_000144"
     CDS             complement(35497..36465)
                     /gene="mdh"
                     /locus_tag="PXD81_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HDL5536219.1"
                     /translation="MCQSKKVALLGAAGGIGQSLALLLKLNLPAKSELSLYDISPVTP
                     GIAVDLSHIPTDVKVTGFAGEDPTEALKDADVVVISAGVARKPGMTRADLFNTNATIV
                     HNLVEKAAKVCPKACIAIITNPVNTIIPIAAEVLKKAGVYDKNKLFGVTTLDVIRANT
                     FVAEAKDVNVKYVRVPVIGGHSGTTILPLLSQATVNGLKLEFTQEQIEQLTHRIQNAG
                     TEVVEAKAGGGSATLSMAQAGAEFALGLVRALIGEDVIRYAYVDNANGETSPAFFAYP
                     IRLGTNGVEKVLPIGNLSEFEKDQLEQLIPVLNDEIQLGQTFNKNA"
     gene            36661..37122
                     /gene="argR"
                     /locus_tag="PXD81_000145"
     CDS             36661..37122
                     /gene="argR"
                     /locus_tag="PXD81_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005713376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HDL5536220.1"
                     /translation="MDKIDSLSEAFKSLLKEEKFGSQSEIVAALQEMGFDNINQSKVS
                     RMLSKFGAVRTRNPKMEMVYQLPVELSVPTTSSPLKNLVVDIDHNDALIVVKTSPGAA
                     QLIARLLDSMGKAEGILGTIAGDDTIFITPVRNVQIEKLVKNVTQLFETSL"
     gene            37172..38068
                     /locus_tag="PXD81_000146"
     CDS             37172..38068
                     /locus_tag="PXD81_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HDL5536221.1"
                     /translation="MHIFITGGTGFIGRELIQQLKSENHHFTLLTRDTEAKSDDLQIN
                     YCYSLDELVDLNDFDAVINLAGEPIFDKRWTDEQKQKLIESRVNLTNKLSQLMNASSN
                     PPHTFLSGSAIGIYGDLPPSANFYTESTACYQDCFTNRLCQQWEAAALKAASDKTRVC
                     LIRTGTVFSPEGGALKKILPLYRCGLGGKLGSGKQYWAWISRRDHVQAVIFLLKNANC
                     SGAYNFVAPNPVRNEEFNRILANAVNRPAFFTVPEFALKLGLGERACLVLDNQQLIPE
                     KLLAASFVFQDPILTENVFLNP"
     gene            38113..40311
                     /gene="uvrD"
                     /locus_tag="PXD81_000147"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             38113..40311
                     /gene="uvrD"
                     /locus_tag="PXD81_000147"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005608557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HDL5536222.1"
                     /translation="MDFSLLLDGLNDKQREAVAAPIGNYLVLAGAGSGKTRVLTHRIA
                     WLIGVENVPESNILAVTFTNKAAAEMRHRIEYTLSQSGDNRLFGMWVGTFHSIANRLL
                     RSHYLDADLPQDFQIMDTEDQQRLLKRLLKLHNIDEKHFPPKHVAWYINAQKDKGKRA
                     KDIDHYNDPNEKKLVEIYQIYQDACDRAGLLDFAELLIRAYELFKTKSTILQRYQQRF
                     QQILIDEFQDTNNIQYDLIRLLAGDVGKVMIVGDDDQSIYGWRGAQVENIQRFLNDYD
                     NAQTIRLEQNYRSTGNILSCANELIANNDNRLGKNLWTDSGDGEPVDIYCAFNELDEA
                     RFVASQIKQWKEDEGDLAECAVLYRSNSQSRVIEEALIQANIPYRIYGGMRFFERQEI
                     KDALAYLRLIANRQDDAAFERVINTPTRGIGDRTLDTLRQITRGRQITLWQAIQVAVQ
                     EEQLSGRATSSLLRFVELINALDQETEQMSLAEQTDFVIKKSGLYEMYKQEKGEKGEV
                     RIENLEELVTATKQFVKPDEAEELTDLTAFLTHASLEAGEAQASQHEDYVELMTLHSA
                     KGLEFPRVFIVGVEEGIFPSGMSFDEGRLQEERRLAYVGITRAKKKLTISYAESRRLY
                     GKEERHVPSRFIAELPEEHIREVRLRGSINRAASFNQANSFAKNPTKTTASFLNDDTW
                     KMGQKVNHAKFGQGTIINVEGSGEATRLQVAFVGNGIKWLIAKMANLERI"
BASE COUNT        11929 a         7765 c         8849 g        11940 t
ORIGIN      
        1 gcaaataata gtgtattaat taagattagt cggcattatt gaaaaaagaa ttaatctgac
       61 tgcttgccga ttttccatta gcaaaaaaat gtaatttgat gtaaactact ttcccgtcct
      121 gatagtttct tttttacgaa gtaaactgat attctgaatt tccaacctaa tcaatcagca
      181 aaggcaatca caatgacaac gaattatatt ttcgtcacag gcggtgttgt atcttcttta
      241 gggaagggta ttgcggcagc ctctttagca tctattctgg aagctcgtgg tttaaatgta
      301 accattatga agcttgatcc ttatatcaat gtcgatccgg gtacaatgag tccaactcag
      361 cacggggagg tatttgtgac cgaagacggt gcggaaaccg atttggattt aggtcactac
      421 gagcgtttta tccgttcaaa aatgagcaaa gccaacaact ttaccagcgg taaaatttac
      481 tctgaagtgt tgcgtaaaga acgccgtggc gactatttag gtgcaaccat tcaggttatt
      541 ccgcatatta ccaacgagat taaagcccgt gtgttagaag gcggtaaagg gcgtgatgtc
      601 gccattgtag aagtaggtgg cacggtgggg gatattgaat cactgccatt cttagaagca
      661 ctccgccaat tagcggtgga tatcggtcgt gaaaaaacct tgtttatgca tttaacctta
      721 gtgccttata ttccaaccgc aggggaagtg aaaactaagc cgactcagca ttcagtaaaa
      781 gagttgcttt caatcggtat tcagccggat gtgttaattt gccgttcaga tcgtgctatt
      841 ccggaaaatg agaagaaaaa aatcgccctt ttctgtaatg tgccggagcg tgcggttatt
      901 tcgcttaaag atgtagattc gatttatcgc attccggaat tattgaaatc gcaagggtta
      961 gattcctttg tgtgcgatcg tttccgttta gattgcaaag aagccgatct ttcagagtgg
     1021 gaacaagtgc tatactgcca agcaaaccca accggcgaag tgactatcgg tatggtcgga
     1081 aaatatgtgg aattacctga tgcgtataaa tcggtgaatg aggctttgaa acacgcaggt
     1141 ttaacgcatc gtttaacagt gaatattaaa tacattgatt cacaagatat tgaaaccaaa
     1201 ggcacagagt tattggcagg cttagacggc attcttgtgc cgggcggctt tggctatcgt
     1261 ggcgtggaag gcaaaattcg aaccgcacaa tatgcccgtg aaaacaagat tccttactta
     1321 ggtatctgtt taggtatgca gatcgcccta attgaatatg cccgtaatgt ggcaggttta
     1381 accgaggcaa attcttctga atttgataaa gattgctcac agccggtgat tggcttaatt
     1441 accgaatggc aagatgcgga agggaatgtg gaaacccgca gcgatgagtc agatttaggt
     1501 ggtacaatgc gtttaggctc acaacaatgc catttaattg aaggctcaaa agctcgtgag
     1561 ttatatggtg cggaaacgat tgttgaacgt catcgccacc gttacgaagt aaacaataag
     1621 ctgttaccac aaattgaagc ggcaggctta aaagtgagcg gtttatcggc agatcgtaaa
     1681 ttagtggaaa ttattgaagt gccaaatcac ccttggttta tagcagcaca attccatccg
     1741 gaatttacct caactccgcg tgatggtcat ccattatttg caggttttgt gaaagctgcg
     1801 aaagatcacc aagagagtcg taaggcttaa tcaaacttaa caagcggtcg aatttactca
     1861 tttttttgta caaacgcttg cgtttgaaca tcggctttgc tgatggcacg aagtgcgagc
     1921 gaaagcgagt aaatttgacc gcttgctcat taattataag aaagggataa atcaatgatt
     1981 tatccctttt agtattttta atagtaagaa tgttcaccgt gttggtgttc ggtaatatct
     2041 ttcacaccaa ctaattcatc agggaacatt gccagaagct gtttctcaat tccctctttt
     2101 aaggtcacat ccaccattga gcaaccgtta caaccaccgc cgaattgtag cacggcatat
     2161 ttatcttcgg tgatttcaat taaggtcact ttaccgccgt ggctggcaag ttgtggattg
     2221 acttgggttt gaatcacata atctaaacgc tcgataaacg gtgcatcatc agccacttta
     2281 cgcattttgg cgttaggggc tttgagggta agttgtgaac ccattgggtc ggttacataa
     2341 tcaatttccg cttcgtctaa aaacggtaag ctaatatcat cgacaaatgc agagaaacgg
     2401 ttaaatttaa attcggtatc cgtctcttca actgcattgg gtgggcagta agagacaccg
     2461 cattctgccc ccggtgtgcc gggattgacc acaaaaatac ggatattagt tccctcttct
     2521 tgctgttcta atagtctggc aaaatgggct tgtgctgcat ctgatattga aatatgaatg
     2581 gtttgttctt ccatttaaat tatcctgttt tttaatattt tacaaaattt atcaactatt
     2641 ttacgcctga atttcaaaat tgcctagcta aaaaaatttt tgcaaattgt taataaaatt
     2701 taaccgcttg aagggaaatt ggattaacta attatggtta atttatgtta agagggaatt
     2761 gaaatcttga tttatttcaa attttaccgc gggaatatgc aaaaaatagg gggttttcct
     2821 agccagaagg ggagcttttt aggtaaaata agaagattat ttttagttaa ccttttgtta
     2881 agaaaagata aaggttgatc acagatttga gaacttaatg tggcttttaa aaataggtgg
     2941 gggaccattg tttattttta agtaaagctg cggctcaata gaagcgattg cgttagcgca
     3001 atcatatttt ttaacccaaa agaaaagtag tgcaaacaat atgattacaa tcaaaaaagg
     3061 cttagatctt ccgattgcgg gaaaacctga gcaagtaatc cataacggta ataccgttac
     3121 tgaagttgct atgcttggtg aagaatatgt gggtatgcgt ccttcaatga aagtgcgtga
     3181 aggcgatgta gtaaagaaag gtcaggttct ttttgaagat aaaaagaatc cgggcgtggt
     3241 atttactgcc cctgcaagtg gtaccgtaac ggcaattaac cgtggcgaga agcgtgttct
     3301 tcaatcggta gtaattaaag ttgagggtga tgagcaaatt acctttgctc gttatgaagc
     3361 cagccaatta gcttcattaa cttcagagca agtaaaacaa aaccttgtag agtcaggctt
     3421 atggacggct ttccgtacac gtccgtttag caaagtgcct gcattagatg cagaaccttc
     3481 ctctattttt gtcaatgcga tggataccaa tccacttgca gcaaatccgg aagtgattat
     3541 tgcagaacac caacaagctt tctttgatgg cttaactgta ttaagtcgtt tatttgaaag
     3601 taaaaaagtg attcatctct gtggcagtgc agactcaaat attttaaatg cggcacaaag
     3661 ccgtcagatc aataatgtgg aaactaaccg ctttaaaggt ccgcaccctg caggtctttc
     3721 tggtacgcat attcacttta ttgacccggt aggtgcaact aaacaagttt ggtatttaaa
     3781 ttaccaagat gtaattgcta tcggtaaatt atttacgacc ggtgagctat ttactgaccg
     3841 tgtagtcgca ttagccgggc cacaagtgaa aaatccacgt ttggtgcgta ctcgtttagg
     3901 ggcgaatctt tcacaattaa cagccaacga attaaatgcg ggtgaaaacc gtgtcatttc
     3961 aggttctgtg ttaagtggtg ctatcgcaac gggagtacac gactatttag gtcgttacgc
     4021 attacaagtg tctgctttat tagaagggcg tgagaaagcg ttcttcggta tgattgctcc
     4081 ttactctgat aagtattcaa ttacgcgtac tactttaggg cattttgcga agaaattatt
     4141 taacttcaca acagcagttc acggtagtga gcgtgcaatg gtaccaacag gggcttatga
     4201 gcgtgtaatg ccgttggata tcattccaac actcttattg cgtgatttag cagctggaga
     4261 tacagactcc gctcaagctt taggctgctt agagttagac gaagaagatt tagcgttatg
     4321 tacttttgtg tgttccggta aaaacaactg gggaccatta ttacgtcaag cgttagataa
     4381 gatcgagaag gacggttaaa aatgggtttg aaaaatcttt ttgaaaaaat ggaacccgct
     4441 ttccataaag gtggaaagta tgagaaatgg tacacgctat ttgaagctgc ctatacgatt
     4501 ttctatacac cgggtacggt cactcgtaaa gatgctcacg tgcgtgatgc gattgactct
     4561 aagcgtatga tgttaattgt atggttagcg ttattccctg cgatgttcta cggaatgtac
     4621 aatgttggtg cacagggtat tctagcggca ctcaaaatgg gaacattaac ggatttagtt
     4681 gctaacaact ggcactacag cttagccgat agtttaggct ctgtagctga tgcaggctgg
     4741 ggaactaaaa tgttgctggg ggcaacctat ttcctaccaa tctacttaac agtatttgca
     4801 gtgggcggtt tctgggaagt ggtgtttgca atggtgcgta agcacgagat caacgaaggt
     4861 ttctttgtaa cctctatctt actagcatta attgttcctc caacattacc gttatggcaa
     4921 gcagcccttg caactacctt tggtgttgtg gtcgcaaaag aagtgttcgg tggcgtgggt
     4981 aaaaacttta tgaaccctgc gttagccggt cgtgctttct tgttctttgc ttacccgggt
     5041 caaatttcag gtgatgcagt atgggttgcc gcagacggct tctcaggtgc aactgcactt
     5101 tctcaatggg cagttggtgg tcaaggtgca ttacagcacg ttgcaaccgg tgaaaccatt
     5161 acttggatgg atgcgttctt aggtaatatt ccgggttcta tcggcgagac ctctacctta
     5221 atgttgatta ttggtgctgc aatcattgta tttgcccgaa ttgcttcttg gcgaattatt
     5281 gccggtgtaa tgattggtat ggcggcaact gcgacattat ttaatgtaat cggttctgat
     5341 accaacccat tattctctat gccttggcat tggcatttag tattaggcgg ttttgcgatc
     5401 ggtatgttct ttatggcaac cgacccggta tcagctgcat ttaccaacaa aggtaaatgg
     5461 gcatacggta tcttaatcgg ctttatgtgt gtattaatcc gtgtggtaaa cccagcatac
     5521 cctgaaggta tgatgttagc gatcttattt gctaacttat ttgctccaat ctttgactat
     5581 ttagtagttc aagggaatat caaacgcaga ttagcgaggg tagcaaacaa tggctaaatt
     5641 taataaagat agcgttggcg gaacgctaac cgttgtagtt ttattaagtt taatttgttc
     5701 tttgattgtg gcaggtgcgg cagtaatgct taaaccaaca caagatattc agaaacaact
     5761 tgataaacag aaaaacatct tacaagcggc cggtttattg caaaaagata ccaatgtgca
     5821 agaaacctat accaaattta ttgagccgaa aattgtcgat ttagcaacag gtgattacgt
     5881 tgagggtgta accggttttg atgctcgtgc agcagctaaa gatcctgctc aaaatgtagt
     5941 tatcgcacct gaagatgata aggcgaatat caaaacgcgt gctaaatatg ctgaagttta
     6001 tttagtaaaa gatgaatctg gcaaaacgac tcaagtagta ttaccaatgc acggtaacgg
     6061 cttatggtca attatgtacg gtttcgttgc ggtacaacct gatgcaaata ccgttaacgg
     6121 tattacttac tatgaacaag gtgaaacggc aggcttaggt ggcgaaattg caaacccgaa
     6181 ctggcagaaa aactttgtag gtaaaaaact gtataacgag caaaaccaag ttgcgttaag
     6241 agtgggtaaa ggggcatctg cagataaaga tcacggtgtt gatggtttat ctggtgcaac
     6301 attaacctcg aacggtgttg atggttcatt caaatactgg tttggtcaaa atggttttgg
     6361 tccatattta gcaaaattta aagcagcggc aggagctaac taatggcaga caataacaat
     6421 cttaaaaaat tattgttatc gccaattgtc gataacaacc cgatcgcatt acagatctta
     6481 ggtatttgtt ctgcgttagc ggtaacgact caattacaaa cagcagtagt aatggcgatt
     6541 gcggtaagtt tagttactgc attctcaagt atgttcattt caatgattcg taactatatt
     6601 ccaaatagta tccgtatcat tgtgcaaatg gcgattattg catcattagt aattttggtt
     6661 gaccaaatct tacgcgctta tgcttacgat ctgtcaaaac aattatcggt attcgtaggc
     6721 cttattatta ccaactgtat cgtaatgggg cgtgcagaag cattcgcgat gaaatcaggc
     6781 ccggttgaaa gttttgttga tggtatcggt aacggtttag gctacggtgc aatgttaatt
     6841 atcgttgcct ttttacgtga acttatcggt tcaggtaaaa ttttcggtat caccattctt
     6901 gaaactgttc aaaatggcgg ttggtatcaa gcaaacggtt tattcttatt agcaccaagt
     6961 gcgttcttta ttatcggatt tgtgatctgg gcgattagaa cgtggaaacc ggaacaagtg
     7021 gagaaataag atggaacatc ttctgagtat ctttgttaag tccatattta ttgaaaatat
     7081 ggcactttct ttcttccttg gtatgtgtac attccttgcg gtgtctaaga aagtttcaac
     7141 tgcttttggt ttaggtattg cggtaattgt agtacttggc attgcagttc cagctaacca
     7201 attagtttat acccacgtat taaaagaggg tgcattagta gaaggtgtgg atttaagctt
     7261 cttaaacttt atcactttca tcggggttat tgcggcactt gttcaaattc tggaaatgat
     7321 tttagacaag tttttcccgg cactttatag tgcattaggt atcttcttac cacttatcac
     7381 cgtaaactgt gcgatttttg gtggtgtatc gtttatggta caacgtgagt ataactttgt
     7441 agaatctgtg gtttatggcg taggtgcagg tacaggctgg atgttagcca tcgttgccct
     7501 tgcaggttta accgagaaaa tgaaatattc cgacgtgccg gcaggcttac gtggtttagg
     7561 tattaccttt attacggtag gcttaatggc gttaggcttt atgtcattct caggcattca
     7621 attataagga gcatatcgtg gatagtaatt ttattttcgg tattatcgca tttacggctc
     7681 tggtattagt gcttgcggtg atcattcttt tcgctaaatc aaaattagtt gattcaggtg
     7741 atattaccat ctcaatcaac aacgatccgg aaaaaggcat tacgctaccg gctggtggaa
     7801 aattactcgg tgctttagca agtaaaggga ttttcgtttc gtcagcttgt gggggcggtg
     7861 gctcttgcgg tcaatgtaaa gtacaagtga aatcaggcgg tggtgaaatt cttccaactg
     7921 agttatcgca catttctaag aaagaagcga aagaaggctg gcgtttagct tgtcaggtaa
     7981 acgtgaaatc ttcaatggac gttgaacttc cggaagagat ctttggtgtg aaaaaatggg
     8041 aatgtaccgt tatctcaaac gataacaaag caaccttcat caaagagctt aaacttcaaa
     8101 ttcctgaagg cgaagaagta ccgttccgtg cgggtggtta tatccaaatt gaagctgaac
     8161 ctcataccgt tcattataaa gacttcgata ttccaaaaga gtaccacgaa gactgggata
     8221 aatttgactt atggcgttat acttcaaaag tggacgagca tattattcgt gcatactcaa
     8281 tggcttcata tccggaagag aaaggcatta ttatgcttaa cgtgcgtatt gctacacctc
     8341 caccacgcaa tccggacgta ccgccgggtc aaatgtcttc ctacatttgg tcattaaaac
     8401 cgggtgataa agtaacaatt tcaggtccat tcggtgagtt cttcgctaaa gataccgatg
     8461 cggaaatggt ctttatcggt ggtggtgcag gtatggcacc aatgcgttca catatcttcg
     8521 accaattaaa acgtttaaaa tctaaacgta aaatgtcatt ctggtatggt gctcgttctg
     8581 agcgtgaaat gttctatgtg gaagattttg atggtcttca agcagaaaac gataacttca
     8641 aatggtatgt ggcactttct gatccattac cgggcgacag agaagattac ttccgtggct
     8701 ttattcataa cgtgctttat gagaactacc tcaaaaacca cgaagcacct gaagactgcg
     8761 aatactatat gtgtggtcca ccggtgatga atgctgcggt aatcggtatg ctgaagagtt
     8821 taggtgtaga agacgaaaac atcttattag atgactttgg tggttgataa accttaactt
     8881 ctgactacaa gcggtcaaat ttgcaaaatt gaccgcttga tttaatttat attattaagt
     8941 tttggtaatt taataatgtg aattttaagg ggaagcaaat ggaacaactg gatattacgg
     9001 tacttgaaaa tgccttcaat tcccttgaga aaacgattgt cagccttgag aatcaagcgt
     9061 ggtttaaccg gcaagaagca attattcaag acactttgat tgcaggagca atccaaaaat
     9121 ttgaatttgt ttacgaattg tcgataaaaa tgctcaagcg tcagctcaaa aacattgagt
     9181 caaatgacac tgatattgat agcaccgagt tccgtgatat tttgcgaatg gctgctcaat
     9241 atgggctgat tgaatcaccg gaagtttggc tggattatcg caaaatgcga aatattactt
     9301 cgcatactta tgatcaaaat aaagcacagc aggtttataa cggaatttca aactttctgg
     9361 aaagtacgta ttttttagtt aaacagctcc aaaaacgcaa tagctatgat tgatttaact
     9421 gagtcacaac gccaacaggt gttggatatt ttcaaaaaat acttgcctga tacacccgtt
     9481 tgggtttttg gctctcgcat taaaggcaca gccaaagcct attcggatct tgatttagca
     9541 ttaatcacca aacaaccgct cacaattcgg caacaaacag agttagagct tgcgtttacg
     9601 gattctgacc tacctttcac agtagatctg attgactggg caagttgcaa cgaatcgttt
     9661 aaacaaatta ttttacagcg atatgaagtg cttatttaat cgctaaatat gctataattt
     9721 ccccaatttt taacttttgt gacaccaatg aaaaaatttt ttcttctttt aattgctttt
     9781 tttactttaa gtgcttgtga aaaagcccct gagcaaatta cgctgtcagg caaaaccatg
     9841 ggaacgactt acaccgtaaa atatattgat aatggtgaag tggagaattt gatttcgcct
     9901 gaaaaaatga aaaatgagct ggatggctta ttgattgagg tgaataatca gatgtccacc
     9961 taccaacaag attcggaaat tagccgtttt aatcaagcaa agcaggcaaa tcaagcggtc
    10021 gaaatttcgc aggattttgc aaaagtggta caagaggcaa tccgtttaaa taaagtaacc
    10081 gaaggggctt tagatgtaac cgttggtcca ttggtgaact tgtggggctt cgggccggat
    10141 aaacgcttaa ataaagtgcc gtctgatgaa caaattgcag agcgttctgc ttatgtcggc
    10201 attgataaaa ttttgcttga aatgagtagt aaacccaagc tcaccaagtc agcagctaat
    10261 ctttatctag atttatcgtc tattgcaaag ggtttcggtg tagataaatt agccgagcat
    10321 cttgaaacta taggtgtgag caactattta gttgaaattg gtggcgaatt acgaggcaaa
    10381 ggaaaaaatt tacaaggctt agattggcgg attgcaatcg agaagccggt aatgacacaa
    10441 ggtcaagccg cacaaattac cgtgccgttg cacaatttgg cgatggcaac ttcgggcaat
    10501 tatcgtaatt attttgaaga tgaaaaaggc aaccgtcttt cacatattat cgacccaaaa
    10561 cagttacgtc cggtaaacca taatttagcc tctatcaccg tatttgcccc aactacaatg
    10621 acggcagacg gcttatcaac cggtttattt gttttaggag cggaaaaagc attagcagtg
    10681 gcagagcgtg aaaaattagc tattttttta attgttaaaa acggcgataa atttgagaca
    10741 caaatgtcga gcgaatttag caaacttatt caatcacaat agttggagtg aaaaatggaa
    10801 actgtattat taacctttgg cttttttgta gccgtaattt ttgcgatgtc gattggtttt
    10861 atcgtgaaag gcaaaaccat caaaggcagc tgtggcggta tcaccgcttt aggtatgaaa
    10921 aaaatgtgcg actgcgaaga accttgcgat aacctaaaat ctaaaatcgc agacggcaca
    10981 gccgaccctg aagaagtggc acgttttaat aaagagccac aattttatga agtgaaataa
    11041 tatgaataga gatattcgct ggaaacagcg gtttgataac tataaaaaat cagtaagtta
    11101 ccttcaagca gaagcagaaa aatacgctga taccgacatt gatgtaatta aaaaaggaat
    11161 tatccagagt tttgagatta cacacgagtt agcgtggaag ttaatgcaag atattttaaa
    11221 atgggaaggt gaagttgata tttatggttc aaaaacggct tctcgtttgg cttttaatcg
    11281 tgggctaatc actcagggcg aagtttggct agaaatgata aaaagccgca atataacagt
    11341 tcatactgac gatgaaacta ttttaactga agaatttaat aaaattatca aggcgtatct
    11401 gcctttattt attgaatttg aacagcgagt agaaacacta tgccaaactt tggactaagc
    11461 gaacgagcga cgaaaactat taccgatatt cttgcgaaat accctgaagt ggagcaagcc
    11521 attatttacg gctcgcgtgc taaaggtaat tatcgtgaag gctcagatgt agatcttact
    11581 tttaaaggaa aagatcttac ttacgatatt gtcaaacgca tttggggtga tttagacgat
    11641 tccgactcgc cctatttatt tgacttgtcg atttatcatc aattaaccaa tgccaacttt
    11701 attgaacata ttgatagagt aggaaaagtc ttttaccaaa gagaactcta atttcttaat
    11761 aggcaagcgg ttcattttgc aaaatttttt gcaaatttca ccgcttgttg tttttattat
    11821 taaccaatgt gcttcgcctt aaccgaagtt acgggagatt tatgacaact caattaacct
    11881 caaaaaccta cgacactcat ttccctaaat taaccccaga acaacttgct gaaaacagca
    11941 agaaaaaagt cattatcggt atgtccggtg gggtggattc ctcggtttcc gcttttattc
    12001 ttcaacagca gggctaccaa gtggaaggct tgtttatgaa aaactgggaa gaagatgatg
    12061 atacggatta ttgtaccgcc gccgccgagc ttgccgatgc tcaagcggtt gccgataaac
    12121 tgggtatgaa actgcataaa attaattttg cagcggaata ttgggataat gtatttgaat
    12181 atttcttggc agaatacaag gcaggaagaa cgccaaatcc ggatattttg tgtaataaag
    12241 agatcaaatt taaagccttt ttggaatatg cggcagaaga tcttggtgcg gattatattg
    12301 cgaccgggca ttacgtccgc cgtacgagcg atgatcaaga tgcgaaactt ttgcgtggct
    12361 tagatgaaaa caaagatcag agttactttt tatatgcgtt aaataaaaag cagatcgggc
    12421 agagtttatt cccagtgggt gaaattgaga agccgattgt gcgagccatt gccgaagacc
    12481 taggattagc aaccgcaaag aaaaaagatt ctaccggcat ttgttttatc ggtgagcgta
    12541 aatttaaaga tttcttagct cgttttctac cggctcaacc gggggaaatc cgcaccgttg
    12601 agggcaaatt agtcggtcgc catgatgggc tgatgtatca cacgcttggg caacgcaaag
    12661 gcttgggtat cggtggggtg aaagggctaa gtgaagatcc gttttatgtg gtggagaaag
    12721 acttaattaa caatgtattg attgtggcac aaggacacga taattcagcc ctgctttcaa
    12781 gtggcttaat tgcgaaacag ctcacttggg tggatatgca gccgttgcgt gaaaatttac
    12841 gctgcacggt aaaaacccgt tatcgtcaag cagatattgc gtgtgaaatc caagtgattg
    12901 atgatgaaac cattcgagtg atgtttgatg aaccgcaaat tgccgtgacg ccggggcagt
    12961 ctgccgtgtt ttacttaggt gaagtttgct taggcggtgg cattattgag tcacaattaa
    13021 aatagttata aataaatatt gaaatatttt gcatatttat gcaaaataag cgaaaagtta
    13081 aggcggtaaa acgccttttt cattttgatc gatacaaaga ataatataca aggaacagac
    13141 tgtggcactt tggggtggac gctttacaca acaagcagac gcaaaattta aatattttaa
    13201 cgattcatta cgttttgatt accgcttggc gattcaagat attgaaggct caatcggctg
    13261 ggcaaaagcc attacttcgg taggtatttt aacggtagca gaattagaac aactaatcgc
    13321 agcattaaaa gaggtgagag cagaggtaga atctaacctt accatcatct taaaagatga
    13381 tgcggaagac attcacagct gggttgaatc taagctgatt gaaaaagtgg gcgatttagg
    13441 taaaaaattg cacaccgggc gtagccgtaa tgaccaagta gcggtggata ttaaaatgtg
    13501 gtgcaaggtg caagcggtgg cattgcaaga aagaatccga gctttgcaag agcgtttggt
    13561 ttccgttgcc gaagaaaatc aagagtcggt gatgccgggc tatacccatt tacaacgagc
    13621 tcagcctgtg accttcgctc attggtgtat ggcgtattat gaaatgctag aacgggattt
    13681 cagccgtttg agtgatgcta ataaacgtat gagcacttgc ccgcttggtt caggggcatt
    13741 ggctggtacg gcttacggca ttgatcgtga cttattagcc cgtgatttag gctttgaaga
    13801 agccacacgc aacagtttag acagcgtgtc agatcgtgac catattttag agctgctctc
    13861 tactgcctcg atcagtatga tgcacctctc tcgctttgct gaagatttaa tcatttttaa
    13921 tagtggcgaa tcaggcttcc ttgaaatgtc ggatcgtgtg acctcaggct catcacttat
    13981 gccacagaag aaaaaccctg atgcttgtga gctgattcgt gggaaatccg gtcgggtatt
    14041 tggggcatta aacggtttat taactacact caaagggtta ccacttgctt ataataaaga
    14101 tatgcaagag gacaaagagg gtatttttga tgcgttggaa acttggcagg cttgtttaga
    14161 aattgcagaa cttgtactgg ttgatattca agtgaatacc gaacgcaccc gagaagccgc
    14221 tcagcaaggt tatgcgaatg cgaccgagtt ggcagattat ttagtggcga aaggcgtgcc
    14281 attccgtgaa gctcatcata ttgttggcga agcggtggtt tacgcaatca gcaaaaaaga
    14341 gccacttgaa gcattaagca ttaacgaatt ccagcaattc catcattcga ttggcgatga
    14401 tgtttaccca attctctcgt tagaatcttg cttagaaaaa cgttgtgcca aaggtggagt
    14461 gaacccacaa cgtgtggcag aggcgattgc ggtagccaaa gccaatttag cttcaaagta
    14521 aaaccggata caagcggtca tattttgcaa aaaaattgaa aaatatgacc gcttgccgat
    14581 taaattttca attttttacg cattaagatt gcaaaatagg cattacctct ttaaacttcc
    14641 gaataatttt tgaattttat ttctactact ttctttaaag agttttatat gacggaatca
    14701 gttaattcta ctcaagctgg tacagcactt tctttagggc aacaattaaa agcggctcgt
    14761 gaagcgttaa atctctctat tacagatgtt gctcaaaaaa ccaacttaaa aaaatcacat
    14821 attgaatcac ttgaaaatga tatctttatt ctacaaaatg ttgccccaac ttttgtgcgt
    14881 ggctatgtgc gtaattatgt gaaatttttg cgtttacctg aatcccttat ttcgagcgtt
    14941 aattatggtg aagtcactat tccaaaagaa gtcactaaag tttcttcagt aaaaccttca
    15001 tcaaattcac aaggtaaaac cttaaagtat ttaacggtat tagtattgct tgcggcatta
    15061 ggaatgacat tactttggtg gtggcaaggg tatcaaaaag aacaggaaaa tcgcgagcaa
    15121 tttgttagct ctgcaccagt tgctgaagtg gcgaataccg ctacaaataa tgatgcttca
    15181 gcacaaactt ccgtgcctgt tcagcaacct attcaggttt cagagcagcc tgctcaagta
    15241 gcagaaacgg tagttgcagc agaaccggca ttaactgaaa aagtagagcc tacacaagca
    15301 gcatcatcac ctgtaacaca agaagatgtt aaagtggtag aagaagttaa acaagctgat
    15361 gccaccactg cacaggttga ggcagttaat gttttacagc aatctcaatc ggaacaagcc
    15421 cctacagaac agcctgttgc ggtaacgaat gatgagcttc gtatcgaaat taccggttca
    15481 caaagctgga ttacagtgcg gggtgctaaa aataaacgtt tagcagaaaa attatataac
    15541 aatggtgaga ctttaagttt caatgagaat gagcaatatc gtttaaccat tggtgcgcct
    15601 gccaatgtaa aagtgtacta taaaggtcaa gaagtaccat tgaaaattga tgggcgtgta
    15661 gctcgtattc atttaccgtt acagtaagct tgatttaacg atgaaacgca cgtaaagcta
    15721 gcatttaacg atagctgtct ttacgagcgt tttctgtttt tataaatttt cacaattagc
    15781 taaaaatatg atacatcaat cacctattaa acgtcgggta tcgaagaaaa tctatgtggg
    15841 taatgtgcct gtaggcggcg atgcaccgat tacggttcag tcaatgacca acacccgcac
    15901 aaccgatgtg gaagccaccg ttgctcaaat taaatcgctg gaacgggtcg gggctgatat
    15961 tgtgcgtgtg tctgtgccga caatggacgc tgctgaggca tttaaagcga ttaaacaaca
    16021 agtgagtgtg ccattggtgg cggatatcca cttcgattac cgcattgcgt tgaaagttgc
    16081 cgaatatggg gtggattgct tgcggattaa cccgggtaat atcgggaatg aagagcggat
    16141 ccgcacggtg gtagattgtg ccagagataa aaacattccg atccgaatcg gggtaaatgc
    16201 cggatcatta gagcgtgatt tgcaggaaaa atatggcgag ccgacaccac aggcattatt
    16261 agaatcggca ttacgccacg ttgatcactt agatcgcctt aactttgatc aattcaaagt
    16321 cagcgtgaaa gcctcagatg tatttctagc ggttgaatct taccgacttc ttgcaaaaca
    16381 gattgatcag cccctgcatt taggtattac tgaagcaggc ggagcgagag ccggttcggt
    16441 gaaatcggcg gtaggcttag gtctgttgct agcggaaggg attggtgata ccttgcgtgt
    16501 gtcattagcg gctgatcctg ttgaagaggt gaaagtcggt tttgatattt tgaaatcgct
    16561 gcgtattcgc tctcgtggga tcaactttat tgcttgccca acctgctccc gccaagaaat
    16621 tgatgtgatt gcgacggtaa acgagctaga acaacgttta gaagacatta tcacgccgat
    16681 ggacgtttcg attatcggct gtgtggtaaa cggtccagga gaggcgttgg tgtctgattt
    16741 aggggtaacc ggcagcaata aaatgagcgg cttttattta gatggcgttc gtcaaaaaga
    16801 gcgttttgac aacgcaaaat taatcgacca attagaagct aaaattcgtg ccagagttgc
    16861 acaacaacat aatcgtatag atattgaaca aataggataa atatagtggc aaaaacaatt
    16921 caagcaattc gaggaatgaa cgactgctca ccaagtgagt ctccattatg gcaatgggtt
    16981 gaacagaaag tgcgtaatgt gctggcaagt tacggctatt cggaagtacg tatgccgatt
    17041 gtagaaagca ctccgttatt tgcccgtgcc attggcgaag taaccgatgt ggtgtcgaaa
    17101 gagatgtaca cgttctggga taacgatgag caattaacgc tccgcccgga aggtacagcc
    17161 ggttgtgtgc gtgcagcgat tgagcgtggt tggatctaca ataatgagca acgcttatgg
    17221 tatatggggc cgatgttccg ccacgaacgt ccacagaaag ggcgttaccg ccaattccac
    17281 caagccggtg ttgaagtgtt tggcattgca aatccggaaa ttgatgcgga gttgattatt
    17341 ctcaccgcac gcctatggaa agagttaggc atcgatcaac atgtttcttt gcaacttaac
    17401 tctatcggct cgttagaagc tcgtgcaaat tatcgctcag cattagtaaa atttttagaa
    17461 aatcacaccg ctttaatgaa cgaagaagag aaagagcgtt tggtgaaaaa cccgctacgg
    17521 attttagata ccaaaaacga agcactacaa aatgtattaa acgatgcccc aaaactgctc
    17581 gattatttag atgatgacag ccgtgaacat tttacccaac tttgtgcctt acttgatgca
    17641 atgggaatcc gttacgaagt caatccaaaa ttggtgcgtg gtttagacta ttacaataaa
    17701 accgtgtttg aatgggtcac ttcagcctta ggctcacaag gcaccgtatg tggcggtggg
    17761 cgttatgacg gcttagtcga acaactcggt ggacacgcaa cccaaggcgt tggctttgca
    17821 atgggcttag agcgtttagt gctgttggta caagaggtga ataaagagat tgatttacca
    17881 aaaacggtgg atgtgtatat cgtattctcc ggtgaagggg caacactaaa tgccttccaa
    17941 cttgcagagc agattcgtag cgaattgcca cacgttggcg taatgacaca ctgttccggc
    18001 ggtaaattcc aaaaacaatt taagcgtgca gataaagtag aagcgcaaat tgcgttagtg
    18061 attggcgaaa gcgaagtggc tgaaaagcaa gttgttatca aagatttacg ttcaggagca
    18121 gaacaaatca cgattgctca atcagagctg attgctgagc tatctaaacg attttaggag
    18181 aaaaagatga gtaacgaata tttaaataaa accgaagagc agcaatttga agatgccaaa
    18241 aattggttta aagagaacgg gacaccgatt ttggtggcga ttattttagc ttgtgcggca
    18301 actgccggct ggaatttttg gaaaaatcac cagcttgaaa cggcacaaaa aacatccgct
    18361 tcataccaaa gcgtgatgga gagttatttg caaaatccaa gcaaaaatca accgcttgtt
    18421 gagaaattta ttgccgataa taaagattca agctatgccg cttttgccca atttgaagta
    18481 gctaaacaag cggtcgaaaa agggaatttt tctgcggcaa aaacagcgtt acagtcagtt
    18541 ttgacttatt cggattcaac cttacaatct atcgctcgtt ttcgtttggc tcaaattgac
    18601 ttccaactaa aatcttacga tgaagcactg gcaagtctgg ctcaaattca agataaagcg
    18661 tgggaattgc gtaagcaaat tctaacaggc gatattcttg cagcaaaagg cgatattcct
    18721 gcggcgaaaa gtgcttatga gcaagctaaa acgaccgcaa atcctcagga acaaacctta
    18781 attgatgtac gtttgaataa cttataaaaa caaagcctga ttgaaataaa atcaatcagg
    18841 cttttttgtt ggtttagtgt tgctgtgaat tataaacgca ttggcataat cacatattcg
    18901 gcagagctgt tgtcgcaatc ttcaattaag caactagaac ttgaatcaac taagctaaaa
    18961 cgcacacgtt ggcattttaa ggtgtttaac acatctaata catagctaac gttaaagccg
    19021 acttccattt ccgggctgtc gtaggcaaca tcaatgatct cttccgcctc ttcttgctcc
    19081 ggattgtttg cggtaatttt gagtaaatta ttgcttaagg ttaaacgcac ccctctaaat
    19141 ttctcattag aaagaatcgc tgcacgcatt aatgcccgtt taagggtttc ggtttccgct
    19201 tctagaatgc gatctgcatt gcgaggcaga actcggcggt aatccgggaa tctgccatca
    19261 atcagtttag aggtaaatac aatgccgttc attgtaatac ggatattgct tgaaccgatt
    19321 tccaaacgca cggtttcatc gttgctggca accaagcggg aaagctcaag tacggctttg
    19381 cgtggcacga taaccgaatg agtcaaaagc tcttgattta gcaccattgt gcaaaccgct
    19441 aaacggtgcc catctgtggc aacggtgcgt aataaattgc cttcggtttc aaatttcatt
    19501 ccgtttaaga aataacgggc gtcttggttt gccattgaga attgggtggc atcaatcaga
    19561 cgggcaaggg tagattgatc aatcgagaaa tccacttccg gtttccaatc catcaaattc
    19621 gggtaatcac ttgccggtaa tgtagctaaa ctaaatttac tacgtcctgc tttgataatg
    19681 gctttatctt cttcaaattg taccgaaatc acgctgtcat caggtaggct tttactgata
    19741 tctaggaatt ttttagctgg aatggtaaat tttccggcaa aatcgaccgc ttgtagcagt
    19801 tgtgcttggg tggttaattc cacttctaaa tcggtaccgg ttaaagatag acgattacct
    19861 tcaatttcta ataaaagatt gttaataaca ggcaaagtcg gacggctact taatacgcca
    19921 cacacctgtt gtaaaggctt aagtaattgt tctctggtaa tggtaatttg cataatcatt
    19981 ccttcatcgg gctaagatga taatttgcgt gttaaatgga tataatcttc ttggataccg
    20041 ctttcggtat cgcgtaattc attaatggtt ttgcaggcgt gcatcacggt agtatgatcc
    20101 cgaccgccaa attcccgacc aatttccggc aagctgtggt tggttagctc ttttgccagt
    20161 gccattgcca tttggcgagg acgggcaatg cttcttgccc gacttttcga tttaatatcg
    20221 gcaactttta tgccgtaata ttcggccaca atgcgttgaa tattttcaat cgtaatcaag
    20281 taatcaaaag aggcaaacag atcttgcaag gtttcacgca ccgcatcaac cgtaatcggg
    20341 cgaagggtca atttttgcca cgcaatcaca cggtttaatg ctccctccaa ttcacgcaca
    20401 ttggttcgga gtttttgccc cattaaatag gcggcatctt cttgcaaatc tgctccacgt
    20461 tctaaggctt tttggcgaag aattgccact cgggtttcca attcaggtgg ctcaatggtc
    20521 gcactaatac cgccgcttaa acgggattta atcgctgtgt caattttatc gatatttttt
    20581 ggcaaaacgt cagaggttag aataatctgc tttctctgct caaataaagc attgagtaga
    20641 tgtaaaaact cttcttgtac tttcggttta ttggcaagaa attgaatatc gtcaatcatt
    20701 aacacatcaa gcgagcggta gaattttttg attttttccg catcgttatt ggaactgttg
    20761 atcgccttaa taatatcacg ataaaaacgt tccaaatgaa tatatagcac ccgagtaccc
    20821 ggtgtgcgtt taactaattc attgccgata gcgtgtaata agtgggtttt gcccaaaccg
    20881 gtgccggcat aaaacgagag tgggttgcaa tggctttcgc ctaaattatc caccacttgt
    20941 tgagccacta atttagctaa ttggtttgag cgaccttgta caaaattatc aaaggtttga
    21001 tttggattta gcccggtttt aaagccggta ttgctgaatt ctgaactctc gttttttaga
    21061 tttgggctga cctttggctg ttctgtcggt ttaatcccaa cttttaacgt aaccaccaaa
    21121 ttatcatttt tcgccaaaaa acgggcaagc tcggtaattt ccgataaaaa tttatctttt
    21181 acccagtttt caacaaattg attttgtgca taaagtgtaa gttccccatt ctcagaactc
    21241 gcttgcagag gacgcagcca agtgctgtaa tcagttggtg agactttcgt ttgtaagtga
    21301 ttaaggcaat cagaccaaag ggaagacaca atttgctcca aaattattgt tattaaaggt
    21361 cataatgata cctgaaaaaa ggggagaaat cttgcaaatt tttgagagat tttatggggg
    21421 cttggaagcg attttaattg gcgaacaagc ggtcgttttt tgttgaaatc ttgcaaattg
    21481 ctaacaaaaa atgaccgctt gcttagtatg atgtgtgatg gagttgatta aatatccgta
    21541 ttctccggca agaacccacc gctttggtga gcccaaagtc tggcgtaaac accgttttga
    21601 gcaagcaatt cttcgtgcgt gccttgttca acgatttcgc ctttatctaa cacgataagt
    21661 ctgtccattg ccataatggt agagaggcgg tgggcaatcg caaccacggt tttaccttcc
    21721 attaattgcg tgagattttc ttgaattgcc acttctactt cagagtctaa cgcactggtt
    21781 gcctcatcga gtaataaaat cggagcgtct tttagcatca ctcgggcaat cgcaatccgt
    21841 tggcgttgtc cgccggaaag ttttacccct cgttcgccaa cgtgtgcatc aaaaccggtt
    21901 ctgcctttgg catcttgcag gtttggaata aattcactgg cggcagcttg ttcaacggct
    21961 ttgatcatct cttcttcagt ggcgtttggg cgaccgtaca tcaagttttc acgcacagag
    22021 cggtgtaaca gcgaggtgtc ttgagttact aaaccaattt gtgagcgaag gctttcttgg
    22081 gtaatatcac ggatattttg cccgtcaatc gtgattgaac cgtcttgaat atcgtaaaaa
    22141 cgcaataata aattggtgag cgttgattta cctgcaccac tacgcccgac taagccgact
    22201 ttttcccccg gttttatagt gaggtcaaaa tctttcagta gcggtttttt cggatcgtag
    22261 gcaaaatcca cattctcaaa tttaatttcg cctttggtta catttaacgg tttcgcatcg
    22321 attttatcta ccacattgtg tggtttggaa agcgtaatca taccgtcttg taccgtacct
    22381 aaattttcaa atagacgagc aaattcccac ataatccatt gtgataagcc tttaatgcgt
    22441 aaagccaaag cggttgaggt ggcaatcgcc cctgcagtta ctacgcttga accccaaagc
    22501 cataaaccga tacccgcagt tgaaattatt aatgccatcg aggttagatt agttacggtt
    22561 tcaattacag tcactaaacg catctgttta tgcactgtca ccataaatcc ttccatcgac
    22621 tctttcgcat aggcagattc acggttgccg tgcgagaaga gtttgacggt ggcaatgtta
    22681 gcgtaagcat cggtaattcg accggtcatt aaacttctgg catccgattg ctcttgagca
    22741 gcttgggcta atttcgggac aaagtagata atggttgccg ctaatgagat gacccataaa
    22801 ataaacggca cgaataacca gccgtcaagt tgtaataaaa tcacgctgga ggtggtgaag
    22861 taaaccagca catacaccaa catatcggca caagtcataa tcacatcacg cacggaaagg
    22921 gcggtttgca ttacttttgc agacactctt cctgcaaatt cgtcttgata gaaacctaaa
    22981 ctttgcccaa gcattaagcg gtggaagttc caacgcaaac gcattggaaa taccccttgc
    23041 agagattgaa agcgaatgct gctggcaagg taaacaaaca ccactgcaag tagggcaata
    23101 aaaaacatac cgataatcgt ccagcctttt tctgcccaaa gttctgttgg cgagtattta
    23161 ttgacccaat cgactaaatc gcccataaag cgaaacagaa ccgcttcgat aataccgaca
    23221 gctgcaacta atacggtcag tagtaagata taaaaccgca ttcctttagt ggcatcgtaa
    23281 ataaaaggaa tgagacctga ttttggagtt ttcggcattt catttggata ggtttccacc
    23341 cttgcctcaa accatttaaa cagtttttca aacataaatt tgtgatttcc gaataaaaat
    23401 gcaggggcaa atagccattt gcccccgaaa aaatgtgaaa aaattatgga ttaagtaatt
    23461 tttcgtctaa ggctaaatct tcgttgcgat ttataccaat gccggcagct aatacgttgc
    23521 gggcaatttg atgtgcttct tttaaagaat gctctttgta agagccacat tgatattcat
    23581 tcagctcagg gattgccgat acatcaggaa ttttatgtaa cgcatcttcc attgatgccg
    23641 tccaagcttt gacaacttct tctgctgaag gcgatccaat cagtgacata taaaaaccgg
    23701 tacgacaacc cattggtgaa atatcaatga tctccacttg ttcgctattg aggtgatcac
    23761 gcataaaacc ggcaaaaaga tgttccatcg tgtgaatccc acgcaccggt agaatatcaa
    23821 tattcggacg acagaaacgg agatcgaaca cggtaatggt atcgcctttc ggtgtggtca
    23881 ttgtttttgc aacacgcacg gctggtgcgt tcattttggt gtgatctact ttaaaactat
    23941 ctaataaagg cattttttac tccatcaata ttattccgca attccttgat cccaagccgg
    24001 ggcttcatca ggtagtttgc gctgtaagaa aaatcgtctg tttggctggg cttgaggctt
    24061 caccacttta gtgtaagggg tagagaactc aataccgcca tttaataact gcgacattgt
    24121 gccggagttc atacttacac cttgcaggct cacgtccata gtgtaacctg aagcttgcca
    24181 gaattgggta ttggttctga ctaagtgctt gtatttatct tgaatattga cataaataag
    24241 cactcgatcg cctaactcac ttagccctaa acggctgact attcccacct gcattccacg
    24301 gtaaagcacc ggtgcttccg gctgaatacc gttagcattg gtggtttcga caataatggg
    24361 gaaaccgttg gcataggtgg tttttaccgt atcggtatct gcaagaacaa attggttttg
    24421 agcttgccct gaaccgacat caaccgtaat gtaattttgt agtgctgcat ctaaattttt
    24481 aaagccggag gtactgattt caggcgagat aacattaaat ttactgccgg cttttgcaat
    24541 taacggatag tatttccttt gaatgtaagc ggtcgctttt atctgttttt ttgcattttg
    24601 tagctcaagt ttttcaattt gacctacggt taagcccata tatttaatgt ccattccttc
    24661 gcttaactta ctcgcatctt ttgcaatcag cgtgagataa gtattgcccg aagtggcttt
    24721 ctccttactt gcataaagcg ttttattgcc ttttgaaccg ccatcatcaa agctgatcgc
    24781 cccttttaag gtacgcatta atggagctgt agtgatattc acgcctttga gtgacacatc
    24841 aacactcgtt gccggctcaa tccagaaacg gcttttctcg gttaatagat gacgataatt
    24901 tggctggata aatagctcaa cctcaaattt ttgttgtttc ggggttattt ttaacacctc
    24961 gccgacctga aaatcacgat atagcaccaa agagccttcg ctgataccag ataatgaatc
    25021 tgccgaaagg gtagtggtgg cttttttatc taaggtagcc atgcctgatt ccgcactttc
    25081 tatatcttta taaagtgcat attgtttttt cgggctaccg tcatttttac cgttaattaa
    25141 gcgaacaccg ccttttaacc attcagatgg tgaaccggcg tgaactctta acccatctag
    25201 ccccaccgac atatcaatgt tggatatcgc aacaaatttg ctgttactcc caatcaggtt
    25261 gcgataaggc ggataaataa tgacatcaaa acgtactttg tctaactcta ggctacgttt
    25321 gacgatttct ccgatttgca catcgctata gtaaatcccc tgattggtat ctaccccata
    25381 ggcttgagga gcaattaagc taaagctaag taaattcggt aaacttaata gataatcggc
    25441 ttcgttttgt accacaaatt ccgattgagg ttctccttcc cccgggtgaa cctcaaaata
    25501 gacaccacgc agcagctcac ctactttcgt cagctgctct gtgtttaagg agaatttcgg
    25561 ctctttgagc aagattttag tttgggaacg tagtaaattt tcataacttg ggtcaattaa
    25621 taaaacccca tcaatctgtt tatgctgccc tgattcttgc tgtggcacac cgttgtcttt
    25681 tacttgcggt aattttagct gcgaaagcat accaatttgc acattttggt gaaaaacacc
    25741 agtctcatta atttttaaat tatgtgtaag cggtagtttt acgttgattt caattccacg
    25801 tttagctgat ttcaaatccg gatatagctc aaatttctgt ccttgctcgg caatttccat
    25861 attatcaggc gaatcaaaag caaccgcacc ttgtactacc gaggcaaggc tgtccatatt
    25921 gattttaata ccttgtaaat ttacatcagc gctaataccg ctaatattcc aaaatcggct
    25981 ctcttttttt actaaatgag cgtatttttg attaatgacg acatcaattt cgattttttt
    26041 ctggtctttg gtaaaacgat aatcggcaat attacccact ggcactttac ggaaataaac
    26101 actcgctccg acagtaatag agcctatgtc atcagagatt aaacggatca gtaaatcgcc
    26161 atcgctcacg gtaatagccg gaggatcttc ttctgcaata aattcatcaa cgaaatcgcc
    26221 ttcaccggga agtagggtaa tatagttacc agaaacaatc gcatctaagc ctgaaacgcc
    26281 agccaaagaa gcacttggtt gcactaacca aaatttagtc tgtttgcgta aaatactttt
    26341 ggcttcaggg tttatttctg cttgcacttc cacttctttt aaattatcga caaaataaac
    26401 acgcttaacc tgaccaatct gcaagccttg gtaacgaata gcggtttttc cggcagtaat
    26461 gccatcacct ttactaaatc gaatcgtgat tgtttcacct tgctctttta aaatttggaa
    26521 aaatagcaaa gcaccaataa taaaagcaac tattggtaac agccaaaaag gagaaatttt
    26581 gcgaggctgc cggatttggg catctacaac agtgtgggta ttttcttcca tcgtcatttt
    26641 aaattaggga aacctatata gaacaatacc gaagacccaa agggacttcg gtaatagaaa
    26701 taagtcaaaa taagaaatat tattttttct catcacagtt ggttaaatta ctgcatttac
    26761 catacagata taaactgtgg ttagtcaatt ccattccgtg tttttcactg atttcacgtt
    26821 ggcgacgctc catatcagga tctttaaatt cgaacacctt accacaatcc atacaaataa
    26881 tatggtcgtg ttcgtggttc atatttaatt cgaatactgc tttattcgca tcgaaattat
    26941 ggcgggttaa aatgccggct tcttcaaatt ggtttaagac acgataaacc gttgctaaac
    27001 caatgtctga gcctttttct aacagaatct tataaatatc ttctgctgca aaatgttgca
    27061 tttcatcacg aatatcttgc attaatgcaa ggatagtcaa acgaggctcg gttactttta
    27121 agcctacctc ttttaaacgt ttaatatttg cttcagacat atatgccact tatttatctt
    27181 tatccaaaat agttattatg cgagttggtc taagcacatt tcttctttca actgctcgca
    27241 ccatttatta acacgttcag tggttaattc aggctgacga tcttcatcaa tacataagcc
    27301 gacaaaagta tcatcatcaa ctaaggcctg agaaacctcg aaagtgtaac cggcagttgg
    27361 ccaatgacca acaatcacac caccatttgg ctctaccaca tcacgcacag tacccattgc
    27421 atcacagaaa tattctgcat aatcttcctg atcaccgcaa ccaaaaattg caacgacttt
    27481 gccgttaaaa tcaatctctt ttagggtagg cataaaatcg tcccaatcgg cttgcgattc
    27541 accataatac caagttggga tcccaaacat cagaaaatcg tatgcttcaa tatcttcttt
    27601 tgtgcttttg gcaatgtctc gaatatcaat taattcagcc cctaattttt tctgaatcat
    27661 ttttgaaata ttttctgtat tgcctgtatc gctaccgtaa aataaaccta caactgccat
    27721 tgctactacc tatttgtgag ataattattt agaattgttt caattaactg cccgcgtgaa
    27781 acaccttgtt cagacgcctg ttcctctaat aactttacta aattcgaatg cagttttaat
    27841 tctacccgtt ttaaaccgga agatttatct cgttttagtt ggttacgttt attgattcga
    27901 agttgttgtt ctctatcaag aggatttgta cgaggacgcc caacttttgg tacagtcgca
    27961 aacagatcta aggttataca atctgaatct tgttttgcca ttttgtttta ctattgggct
    28021 ttagctaatc ctcagtttta atgagaatga taagctactt ttaaagtgtg aactatatca
    28081 aaagttcaat catttgaaaa gtaaattaca ggcaaaacaa gcggtgtttt taggtttaaa
    28141 atttgcaaaa accataaatt acacattatg agttatgatt tttctgaagc gtgaaattag
    28201 cttataatga aaccgaataa cgttatgaaa aaggagatag tgtgtcgaat atccatttag
    28261 cgattgccgc tgattttacg ctggcagaaa aattacttga agcattagca aatcaagatt
    28321 tagggattga aaatatttca gccgttgagc ttgagccgtt tggtgaagag cagaatttac
    28381 gctttggggc aaaagcagtc gcacaatatg cgttagatga agtaaattgg gcggaattta
    28441 gccatgttct atttgccggc aaaatggcac aagcggaagt gttggcagaa gcggtgaaag
    28501 ccggctgtgt gattttagat ttatacggca ttaccgcact catcggcgat gtaccggtag
    28561 ttgtgccaag tgtaaatgat gaaaatgtcg caaatttgcg tgagcgaaat atcgttgctt
    28621 tagccaatcc gcaaatttca caactggcgt tagccttaaa gccattgttg aacgagccgt
    28681 taaaacaggt gtgggtaacc tcacttttac cgtctgccta ttttggcgat gaaaaagtca
    28741 aggagcttgc cggacaaact gcccgtttgt taaacggcat tccgtttgat gaaaatgcgg
    28801 aacgcattgc ctttgatgtt gtgccggcta atgtgcaagg agaagagaaa agtctgccgt
    28861 tctcaaaagc ctttgaactg caaattgcaa aagttttacc aaatttgacc gcttgtacta
    28921 ccttccacag cgtgcaagtg ccggtctttt atggggtttc ccaaatggtg tcggtgcagt
    28981 ctgaatatca gttagatgct aatgcgttaa gtgaagcttg gaaagccgaa aattggctga
    29041 attttaatga aaattcagtg attacagcgg tgaaaaatgg cgaaaacgaa gagcaaaatg
    29101 cgttagaaat cagtcagtta ttggcgaaat ccgaacagga agtgcagttc tggacggtcg
    29161 ccgatgaaca gcgtttcagt ctggcatttt tagcggtgaa attactaaaa ttagttttag
    29221 cgtattaagt gcttgcaaaa gtatttattc cgtgataaaa atcgtcaacg cccgagcaac
    29281 cgattgcttg ggcgttgtca ttttgtttaa acaccgaagg tcttatgtta gaaaaattat
    29341 ttcagctcaa agcaaaaggc agtaatgtaa aaaccgaaat tatcgccggt attactacct
    29401 ttttcacaat ggtttatatt gttttcgtca atccgtctat tttaggcgtg gcgggaatgg
    29461 acacccaagt agtgttcgtg actacttgtt taattgccgc ttttggtact atcgcaatgg
    29521 ggttattcag taatttaccg attgctcttg caccggcaat gggcttaaat gccttttttg
    29581 cattcgtagt ggtgcaaaaa ttaggttact cgtggcaagt gggtatgggg gcgattttct
    29641 taggctctgt cggcttattc ctattaactg tattacaagt tcgttactgg tttatgtctg
    29701 cgattccact tggtttacgt gtaggtattg gtgccggtat cggtttattc attgccctaa
    29761 tcggttttaa aaatatgggc ttggtggtgc caaatccggc aacgcttgtg gcactaggtg
    29821 atttgcacga cccgaaagtg ttaatgggga ttttaggctt tttcatcatt gttgtattag
    29881 cggcgaaagg tattcactca ggcgtgttaa tttcaattgt gattgttacc gctcttgcct
    29941 tagcctttga cccggcggtg agctttaacg gcataatgtc tatgccacca agccttgggg
    30001 cagttgtggg gcaggttgat attgccggtg cttttgatgt tgccttactc gggatgattt
    30061 tctcctttat gttggtaaac ctgtttgact cttccggcac cttaattgcg gtgactacca
    30121 aagcaggctt tgcggatgaa caagggcgtt tcccgaaaat gaaacaagct ctgctagtag
    30181 atagtaccgc tgcaatggca ggctcttata tgggaacttc agcgatttct acctatattg
    30241 aaagtggctc cggcgtttca gttggcggtc gtaccggctt aactgcggtg gtagttggga
    30301 tcttattcct attaaccatt ttcttctcac cacttgcagg gttagtacct gcctatgcaa
    30361 ctgcgggggc attggtattt gtaggaattt taatggcatc tagcttaatt gaagtaaaat
    30421 gggacgattt aactgaagct acgccggcat ttattactac cgcaatgatg ccgtttactt
    30481 actccatcac tgaagggatt gcattcggct ttatcagcta ctgcgtaatg aaagccttta
    30541 cagggcgttg gaaagaaatc aacctttctg ttttagtggt ttcacttctc tttattgcta
    30601 aatttgtgtg ggtgggttaa gctctacccc cctctccctg atttttcttc taaacgaaga
    30661 aaaatctatc cctctcccac aaaggggaga gggtatttga ttcattatct atgtgtttcg
    30721 tgtattccga ggtaaaacaa tgtctaattc agttcaaaaa cttcttttta tcatcaattc
    30781 tgctccttac ggcaatgaaa cgtttttcag tgcgttgcgt ttggcattac aaattcaaga
    30841 acaacataaa gccaatacgg aaattaaact tttcttaatg tcggatgcag tcaccgcagg
    30901 gctggcaaag caaaatccgg cagagggtta tcatattcag caaatgctgg aaattttaac
    30961 cgcccaaggg gcaaacgtaa aattatgcaa aacctgtact aatgctcgag ggattacaga
    31021 tttgccttta gccgatgggg tagagattgg cacattaatt gaactggcag attggacaat
    31081 tcaagcagat aaggtactga atttttaatg caaaatattg atgttgtggt gattggtgcc
    31141 ggtgcggcag ggctgttttg tgctgcccaa ctggggcaag ctggcaaaaa agtggtggtg
    31201 ttagacagcg gtaagaaagt cggacgtaaa attttaatgt ctggcggtgg tttttgcaat
    31261 tttacgaatt tagacgtaag tgccggaaat taccttagcc aaaaccgcca ttttgtgaaa
    31321 tccgcccttt cacgttatac ccaatgggat tttatttcgc ttgtcagcca ctatggcatt
    31381 agctatcacg aaaaagagct agggcagttg ttttgtgatg aaagctcgca acaaattgtc
    31441 gatttactac aagcagaatg caacaaaggc aaggttgaga ttctgttacg gcaagcggtc
    31501 atttctgtgg aaaaagttgc aaattttttt gaaatttcga ccgcttgtgg caagttccaa
    31561 tccgaacact tagtgattgc aaccggaggg ctttcaatgc cggctctcgg ggcaagccct
    31621 tttggctatc aacttgccga gcagtttggt attcctgttg tgccaccacg tgcaagcctt
    31681 gtgcctttta cttggaaaga gagcgataaa tattttgccg agctttccgg cattgcactg
    31741 ccgattactg tttccgccaa tggcaaaagt tttagcaatc agatgttgtt tacgcacaga
    31801 ggcttatccg gcccggcaat tttgcagatt tccaattatt gggaattggg ggaaacggta
    31861 gagattgatc tgctaccttc gcagtccatt accgatatat tgagcgaact acgccaatct
    31921 tcgccgaaat tacagctaaa aaccgtatta tctcgttatt tacctaaaaa attggttgaa
    31981 gtttggctgg aacagaaatt gctgaccgat aaagtgattg ccgatttgag taaaaaagat
    32041 ctgaccttat tagagcaatt tatccaccat tggcatttct tgccaaacgg cacggaaggc
    32101 tatcgtacag cggaagtaac caaaggcggt gttgataccg actatatttc atctaaaacg
    32161 atggaagcga aaaatacagc agggctttat tttatcggcg aagtgctgga tgttaccggc
    32221 tggcttggtg gttataattt ccaatgggcg tggtcatctg cttttgcctg tgcagaaggg
    32281 attattgaaa aaagcaaata actaaaaaag caaataacta aaaaaccaaa caaaaaggcg
    32341 aacacaattg ttcgccttat tttatgctga attatcttgc acggaaaata atgcgtgctt
    32401 tgcttaaatc gtatggagtc atttcaaccg ttactttatc accggttaaa atacgaatgt
    32461 agtttttacg cattttgcct gaaatatgtg cggtaacaat atgaccgttt tctaattcca
    32521 cacggaacat tgtatttggt aaggtttcta aaatcgtacc ttgcatctca atgcaatctt
    32581 ctttagccat ttattcctct ttaaagtttt aatttttata tgatgccatt cggcgaaaaa
    32641 cggcggatat tatacgctta ttcgctcaaa ttacaatcgt ttatccattt gagtgtaaac
    32701 tatttacgct taatccaacg gcgacgtgag tgaaaacgct gggtttgggt ttttacttga
    32761 ctcataagtg gttttgggct tatctgtaca tgatgaattt tacgacgaaa atagtagtta
    32821 atgctacttg ccaacaatat acctgtgagt agcaaaataa ttatttcccc ataaagttga
    32881 tgaaattgtt gctctactcg tgctttagta gtttgcccat attctgcatc aaacgtgacg
    32941 cgtaaaaagc caaccaacgc ctgttgagaa aatattggct caacaatttg ctgagttttg
    33001 gcagattgct ctgaatcgag caatactttt aaatctttcg cattttggct ttgagccagt
    33061 agcacgccat ttggtgaata gatattggca tcaataataa aatcttcttt ggcaaaacta
    33121 tccaacgcat caaccagctc atcggttttg gcatttttaa ctaacaaaaa cgaaaaaata
    33181 tttgcttgct ggcgaaccaa taggtgtgaa aggtttgata cttgattaat gctggccaat
    33241 tgtgagccta gcttaaattg gtttacgccc attaaaataa ccgccacgat cccacagcag
    33301 agtagcgtaa tccctgccag caaaagcgtt ttcgttattt tttcacgact aatgtacatc
    33361 gaaattccct caaaatgatg cgtgattgta gcacgctttt ctgttagatt agtgcagtaa
    33421 atttaaacaa cctaaaataa gacaatgaca aacacaattt ttatctattc aaaaacctta
    33481 aacgagcgtg aaattgaaca gttttcacaa caaacggcag caattttgct gaaacaagcg
    33541 gtctatttag actacaaaat tgcatttttt tccacaaatt taaccgcttg cgagctgcgt
    33601 gaaacggcac agaaaatcaa tgcggatatt agcgatctca ctattattcc aaccttaaac
    33661 caaggcggtt tgctattaat ggatatggat tccaccgcta ttaaaattga atgtattgat
    33721 gatattgcca aacttgccgg caccggcgag gtggtgtccg ccattactgc cagtgcaatg
    33781 cggggcgagc tggatttcga gcaaagttta cgcaagcgag tcggcacgtt ggaaaatgca
    33841 tcggaaagca ttttgcaaaa agtgcgggaa aatctaccgc ttatggacgg ctttgagcag
    33901 atggttgcta ttcttaagga acacggctgg aaattagcga ttgcgtccgg tggatttgat
    33961 tacttcgccg attacttaaa acaaaaatac gggttgaatt ttgcggtctc taaccaactg
    34021 gaaattattg acggtaaatt aaccggcaag gtgttaggca aagtagtgga tgctcaacat
    34081 aaagccgata ccttacagaa tttagcgaca acctttaata ttccaccaaa tcaatgggtg
    34141 gcagtcggcg atggggcaaa tgacttacca atgttaaaaa ccgccagttt aggcgttgct
    34201 ttgcacgcta aaccaaaagt gcaagcacaa gctgattttg tggtaaactt cggggactta
    34261 acggcattat gtttattact taatgccaat catttatttc aacattaatt gagaggaaca
    34321 acaatgccat catttgatat tgcatctgaa atcacgatgc acgaagtgcg taatgcggta
    34381 gaaaatgcca atcgtgtatt aagcacccgc tacgatttcc gtggggtgga agccgttatt
    34441 gagttaaatg aaaaaaatga aagcattaag ctcacgaccg aatcggattt ccaattagag
    34501 cagttaattg agattttaat cggctcttgt gtaaaacgtg ggatcgagca tagttcatta
    34561 gatatcccta cagaaagtga gcatcacggt aaactctatt ccaaagaaat taagctcaag
    34621 caagggattg agtcagatat ggcgaagaaa attaccaagc tgattaagga ttctaaaatc
    34681 aaggttcaag cccaaattca aggcgagcaa gtgcgtgtaa cgggcaaatc tcgtgatgat
    34741 ttacaagccg caattcagct agtgaaaggg gcagatttag ggcagccatt ccaatttaat
    34801 aatttccgtg attagcaatt cctgcaatta atgatgacaa gtggaaaaat tcagcaaaat
    34861 ttgaccgctt gcattaataa ttcaacataa aaggaagaga tgatgagcga gcaaacagaa
    34921 aagaaaggct ggtttaaaaa agcccttgag aaatatgatg ccttttgcga tgagctaggg
    34981 gtaaatcaag gtgcttgccg aggttgtgtc cctgtggtga agtttgaccc tgaaccggaa
    35041 aaagagcagc cagaaaaatc agaaaataaa acatagaaaa aatgcacaat ggtaagatac
    35101 attgtgcatt ttttatagga tttttaattg gcgtgatgat gggcaaaata ttgcttaact
    35161 ccgccatctt tcttgcaagg attctgaata tcagtagagt aactaccgtt aaaacaaaga
    35221 gcagtagcat tcgatggttt aatcttttcc tgtttagtta gggctggcgg ttgagtattg
    35281 gtttcactaa tatagatcgt tttaggttga gaatagtcta tatttttgtc gccatttacg
    35341 acttgaacgg gggtgttcga gctgcaagct acaaccgatg ctgaagccaa tgtaagtata
    35401 atgatatggt gttttttcat gggtaatttc cgtttttata tttttaattg agagaatgtt
    35461 aaaaagggct gatttctcaa cccttaaagt tatgccttaa gcatttttat taaaagtttg
    35521 tcctaactga atttcatcat ttaatacagg aattaactgc tctagttgat ctttttcaaa
    35581 ttcgctgaga ttgccgatag gtagaacttt ttccacacca tttgtaccta aacgaatcgg
    35641 gtaagcaaag aatgccggag aggtttcgcc attggcatta tctacataag cataacgaat
    35701 gacatcttcc ccgattaagg ctctcactaa accaagtgca aattctgctc ctgcttgagc
    35761 catagataag gttgctgagc caccacctgc ttttgcctca accacttcag taccggcgtt
    35821 ttggatacga tgagttaatt gctcgatttg ttcttgagtg aactcaagtt ttaagccatt
    35881 tactgttgct tgtgaaagca gtggaagaat agttgtgccg gaatgtccgc caatcacagg
    35941 cactcttaca tatttcacat ttacgtcttt cgcttcagcc acaaaggtat tcgctcgaat
    36001 aacatctaat gttgtaacac cgaaaagttt atttttgtca tacacaccgg cttttttcaa
    36061 tacttctgcc gcaatcggaa taatggtgtt taccgggtta gtaataattg caatgcaagc
    36121 tttagggcaa actttcgctg ctttttcgac taagttatgc acgatagtgg cattcgtatt
    36181 gaataaatcg gcacgtgtca tccccggctt acgggcaaca ccagcagaaa taacgaccac
    36241 atcggcatct tttagtgcct cagtcggatc ttcacctgca aaaccggtta cttttacatc
    36301 agttggaata tggcttaaat ccactgcaat tcctggagta acaggggaaa tatcgtaaag
    36361 agataattca gatttagccg gtaaattgag ttttaataat aaggcgagtg attgaccgat
    36421 tccacctgca gcacctaaaa gggcaacttt tttactttga cacataaaat ttctcctaaa
    36481 tgttatggaa gtttagacca atttagtcta agccaatttt ttgggcgatt caaacggcaa
    36541 atctcatttt tgagacatag agcaaatttt tagctgattt ttatgggttt tattgattta
    36601 tttgcataaa taagcaagaa ttatgaatat ttttaagttt ttgtgaataa gagtgtgaaa
    36661 gtggacaaga tagatagttt atcagaagcg tttaaatcgc tgttaaagga agaaaaattc
    36721 ggctcgcaaa gtgaaattgt tgccgcacta caagagatgg gctttgataa tatcaaccaa
    36781 tccaaagttt ctcgaatgtt atcaaaattt ggtgcagtca gaacccgtaa tcctaaaatg
    36841 gaaatggtgt atcagctacc agtcgaatta agtgtaccga ctacctccag cccacttaaa
    36901 aatttggtgg tagatattga tcataatgat gcgttaattg tggtcaaaac cagccccggt
    36961 gcggctcaac tgattgcacg tttgctcgat tcaatgggta aagccgaagg tattttaggc
    37021 actattgctg gcgatgatac gatttttata acgccggtac gcaatgtgca aattgaaaaa
    37081 ttggtgaaga atgtgacaca gttatttgaa acatcattat agaataagta aaggaggact
    37141 taatgttctc cttatttttt aggagattat tatgcacatt tttataacag gtggtacggg
    37201 ttttattggg cgtgaattaa tccagcaatt aaaatctgaa aatcaccact ttacacttct
    37261 tactagagat acagaagcga aaagtgatga tttacagatt aattattgct attcacttga
    37321 tgaacttgtt gatttaaatg attttgatgc agtaattaac cttgccggtg agccgatttt
    37381 tgataaacgt tggacagacg aacaaaaaca gaaattgatt gaaagccggg ttaacctaac
    37441 gaataagctc agtcaattaa tgaatgcaag ctctaaccct ccgcatactt ttctctccgg
    37501 ttcggcaatc gggatttatg gcgatttgcc tccttctgca aatttttata cagaatcgac
    37561 cgcttgttat caagattgct ttaccaaccg gctttgccaa cagtgggaag ccgctgcact
    37621 aaaagccgca agtgataaaa cccgagtgtg tttaattcgt accggcacgg tattttcgcc
    37681 cgaaggcgga gcattaaaga aaattttacc gctttatcga tgcggattag gcggaaaact
    37741 tggttcaggc aaacaatatt gggcgtggat cagccgccgg gatcacgtac aagcggtcat
    37801 ttttctgctc aaaaatgcaa attgttcggg tgcttataat tttgttgcac caaatccggt
    37861 gagaaatgaa gaatttaatc gtattttagc gaatgctgtt aatcgcccgg cattctttac
    37921 tgtgcctgaa tttgccttga aattaggctt aggcgaacga gcttgtttag tcttagataa
    37981 tcaacagctc attccggaaa aactactcgc agccagtttt gtattccaag atccgatttt
    38041 aaccgagaac gttttcttaa acccataatt taggctataa tgctgtataa aagcacagat
    38101 taaaaggtga ttatggattt ctcattatta ttagacggtt taaatgacaa gcagagggag
    38161 gcggttgctg ccccgattgg taattattta gtgttagccg gggcgggttc gggcaaaact
    38221 cgggttttaa cccatcgcat tgcgtggctg attggggtgg aaaatgtgcc ggaatccaat
    38281 attctggctg ttacctttac caataaagcg gcggcggaaa tgcgacatcg gattgagtac
    38341 actctttccc aatcgggcga taatcgattg ttcggaatgt gggttggtac tttccacagc
    38401 attgccaacc gcttacttcg ttcccactat ttagatgcgg atttaccgca agatttccaa
    38461 attatggata ccgaagatca gcaacgttta ctcaaacgtt tactgaaact gcataatatt
    38521 gatgagaaac attttccgcc aaaacacgtt gcgtggtata tcaacgccca aaaagataaa
    38581 ggcaaacggg caaaagatat tgatcactat aacgatccga atgaaaaaaa attggtggag
    38641 atttaccaaa tctatcaaga tgcctgcgac cgagccggtt tgttagattt tgccgagctg
    38701 ttgattcggg cttatgaact attcaaaacc aaatcaacca ttttgcaacg ttatcaacaa
    38761 cgttttcagc aaattttgat tgatgagttt caagacacca acaatattca gtacgactta
    38821 attcgtttgc ttgccggcga tgtcggcaaa gtgatgattg tcggcgatga tgatcagtct
    38881 atttatggtt ggcgtggggc acaggttgag aatatccaac gtttcttaaa tgattatgac
    38941 aacgctcaaa ccattcgctt agagcaaaat taccgctcta ccggcaacat tttaagctgt
    39001 gccaatgaat taattgccaa taacgataac cgcttaggca aaaatttatg gacagatagc
    39061 ggtgatggcg agcctgtgga tatttactgt gccttcaacg aacttgatga agctcgcttt
    39121 gttgcctcgc aaatcaagca atggaaagaa gacgagggtg atttggcgga atgtgcggtg
    39181 ttgtatcgca gcaacagcca atctcgtgtg attgaggaag ctttaattca agcgaatatt
    39241 ccttatcgca tttacggtgg aatgcgattc tttgaacgcc aagaaattaa agatgctctg
    39301 gcttatttac gcttaatcgc caatcgtcaa gacgatgcgg cgtttgaacg ggtgatcaac
    39361 acgccaacca gaggcattgg cgatcgtacc ttagacacac tccgtcaaat tacccgtggt
    39421 cgccaaatta cgctgtggca agcaattcaa gtggcggtgc aagaagaaca actttcaggg
    39481 cgtgccactt cttccttatt gcgttttgta gagctgatta atgctcttga tcaagaaacc
    39541 gagcagatgt cgcttgccga acaaaccgat tttgtgatta aaaaatcggg tttatatgaa
    39601 atgtataagc aagaaaaagg cgaaaagggc gaagttcgga ttgaaaactt agaagagtta
    39661 gttaccgcaa ctaagcagtt tgtgaaacct gacgaagcgg aagagctgac cgatcttacc
    39721 gcatttttaa cccacgcctc acttgaggct ggagaagccc aagcctcgca acacgaagat
    39781 tatgtcgaac tgatgacgct acattccgcc aaaggattgg agtttccccg tgtgtttatt
    39841 gtgggcgtgg aagaagggat tttcccaagc ggaatgagct ttgatgaagg caggttgcaa
    39901 gaagaacgcc gtttggctta tgtcggcata acccgagcta agaaaaaact gacgattagc
    39961 tatgccgaat caagacggct ttatggtaaa gaagaacgcc acgttccgtc tcgctttatt
    40021 gctgaattac ctgaagagca tattcgagaa gtgagattga gaggctcaat taaccgagcg
    40081 gcaagtttta accaagctaa ttcatttgca aaaaatccga caaaaacgac cgcttccttt
    40141 ttaaacgatg atacttggaa aatggggcag aaagtgaacc acgctaaatt tggacaaggc
    40201 acgattatta atgtggaggg ctcaggtgaa gccacccgtt tgcaagttgc ctttgtcggc
    40261 aacggcatta aatggctgat tgccaaaatg gcaaatttag agaggattta ggcgaataaa
    40321 aagttttttg caggaatata gatggcgaga tatattgccc cttaacctta aactaagggt
    40381 aaataaaatt aaataatgca agctagatat aatgtaaagt atcagaaata agatattaat
    40441 ttaagataaa gaatagatgg ctggggtact aggattcgaa cct
//
LOCUS       DANLYI010000004        36239 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.004, whole genome shotgun sequence.
ACCESSION   DANLYI010000004 DANLYI010000000
VERSION     DANLYI010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 36239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36239
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.004"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            complement(5..625)
                     /locus_tag="PXD81_000148"
     CDS             complement(5..625)
                     /locus_tag="PXD81_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HDL5536223.1"
                     /translation="MCRFPLFETLSIIDGKVQNLQYHQQRFEQATQEYFGCEPRFSLA
                     DILVVPEASQRGKVRCRIDYNATEFELKFFIYSPKKVERFRLVEVENWDYHLKFSDRK
                     RFDLLPILQNEEVIILNNGYVSDCSIGNLLFLKQGIWYSPQHYLLKGTQLSKLLDEGK
                     VVLAKIAKQDLHQYEKIMLINALNPFEEPRALPCSQIYFCDLDRKS"
     gene            complement(603..1577)
                     /locus_tag="PXD81_000149"
     CDS             complement(603..1577)
                     /locus_tag="PXD81_000149"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005612571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HDL5536224.1"
                     /translation="MQDFIHQANHYGQAKRPFFFLIDFEQQKPLIFPLEQAHASGLFF
                     EFFCKSNLEKPVEKPTKAFELSANPIPFSEYQTGFELVKKEIQAGNSYLLNLSYPTAI
                     ETNYSLAEIFAASKAKYKCYLQGRFVCFSPECFVRIENNRIFSYPMKGTINANEENAE
                     QKLMASEKEFTEHNTIVDLIRNDLALVAKNIEVTKYRYVEKVETHRGAILQTSSEICG
                     ELGENWQENIGSILAKLLPAGSISGAPKEKTVSIIQQAELGKRGYYTGIFGYFDGESL
                     ESAVAIRYIAQTSNGLVFHSGGGITSQSVLTDEYNELLEKVYVPISAI"
     gene            1687..3294
                     /gene="rmuC"
                     /locus_tag="PXD81_000150"
     CDS             1687..3294
                     /gene="rmuC"
                     /locus_tag="PXD81_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005601633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HDL5536225.1"
                     /translation="MHFQEIGIYIALAVCVLVGVYLFFLKSRYQRNAYELSQDLGKIT
                     ENFEQIQQKYEVLAQEKNQLEQWAIQQQTKYEAVSERLNERDGQLQRFQQKIELAEQQ
                     ENQLERYINELKERVGSSQAKAESLEEQLQFSQSSLSTKERENQALFSRLNEVQNELT
                     ELRTTLSEKQANFEAQQRNFIEVKQQLNVEFQHLAQQILDEKSKRFSETNQSSLEALL
                     KPFKEQIEGFQKRVNEVHSESLKGSANLEAEIKRVLQIGVSMSEEAQNLATALKGNNK
                     IAGNWGEVQLESALQSAGLLAGEHYQAQESFRDEEGRRFAPDFVVHLPDQKHLIIDSK
                     VSLVAYDQAVRSEENFAISQALDEHCRSLRNHIEGLSKKNYSALLGIKSPDFVLMFIP
                     IEPAYIEAMKHDPQLFNFGYERNVILVSHTTLMPILRTVANLWRIERGNSEAREISEK
                     AGEIYNQVCAVADRLAKLGNTLNTANNQYNQTVTSLVGRQGLLGKVERFQHLSSKASQ
                     IVPQVELIENEVDTIKLEMVSRELLEE"
     gene            3357..3941
                     /locus_tag="PXD81_000151"
     CDS             3357..3941
                     /locus_tag="PXD81_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component II"
                     /protein_id="HDL5536226.1"
                     /translation="MLKKLLIIDNHDSFTFNLVDLLRKLDVPAKVVLVEELDLNEVEH
                     FSHILISPGPDVPRAYLQTFAMLERYHQTKSILGVCLGHQTICEFFGGKLYNLNDVRH
                     GQQRTLTQIEPNPIFKGLPKQLKVGLYHSWGILQNSLQNTPLVPTAVCDQNVLMAFKH
                     QHLPIFGVQFHPESFITEFGQQMLANWLEITQDF"
     gene            4038..5120
                     /gene="prfA"
                     /locus_tag="PXD81_000152"
     CDS             4038..5120
                     /gene="prfA"
                     /locus_tag="PXD81_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005713004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HDL5536227.1"
                     /translation="MKDSIINKLESLSERHEELQALLGDASIISDQDKFRAYSKEYSQ
                     LEEVVGTFNRWKKLNNDIEEAQLLLDDPDMKDMAAEEIAENKAEIENLEQHLQILLLP
                     KDPNDEYNAFLEIRAGTGGDEAGIFAGDLYRMYSRYCESKRWRIEEMSANESEQGGYK
                     EIIVKISGEGVYGQLKFESGGHRVQRVPKTESQGRIHTSACTVAVMPELPESEMPEIN
                     PSDLRIDTYRSSGAGGQHVNTTDSAVRITHIPTGIVVECQDERSQHKNKAKALAVLAS
                     RIVQVEQERQAAEQADTRRNLLGSGDRSDKIRTYNYPQGRVTDHRINLTVYRLDEVMN
                     GKIDELIQPIITEYQADQLAALSEQN"
     gene            5232..5834
                     /locus_tag="PXD81_000153"
     CDS             5232..5834
                     /locus_tag="PXD81_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5536228.1"
                     /translation="MRKLGLLAVFTLVLSACSNSTKIEQTILSKQDIVGSWVCAIKYD
                     DLKVSTLDFSEFKANGEMTNFGDISDENFEPIKFTYVTGDKGKWDLKGNRLVIDYDLS
                     ERKVKRTTPKKFLEFLKQEERKASKTAKKLLKYEQGIFNILSDQKNTENSKIILDILK
                     FGHSRMAIQQNMGDKVYSGGCVTADKAEEYLDILKNRNSK"
     gene            5834..6700
                     /gene="prmC"
                     /locus_tag="PXD81_000154"
     CDS             5834..6700
                     /gene="prmC"
                     /locus_tag="PXD81_000154"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005616471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HDL5536229.1"
                     /translation="MDYREWLTFAEQKLLENSGADPYLNAKFDANLLLQAVTKRSKSA
                     IFAFSETLLSESELAELAEKLARRAKGEPMAYILGYREFWSLPLKVSPATLIPRPDTE
                     RLVELALEYANKRLGNQKMLQILDLGTGTGAIALALASELGEKAQIIGVDFQNEAVQL
                     AEENRRNLGLNQVTFLQSDWFSSLANQQFDLIVSNPPYIDESDENLTVGDVRFEPLTA
                     LVADNKGLSDLQKIIENAPLHLKPQGALMLEHGWKQGNLVRDLFDLHLWEQVETAQDY
                     AGNDRVTKAILK"
     gene            6709..7575
                     /locus_tag="PXD81_000155"
     CDS             6709..7575
                     /locus_tag="PXD81_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015674537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB1 family protein"
                     /protein_id="HDL5536230.1"
                     /translation="MDDIIEELLKELEDPKVTISETELKKFLYREILRLTTLIDKNVS
                     EQQVFGQMSALVKKARYNVNGEGDEERINQLLHLVYREWGFHCYYEDYFHTENLLLNQ
                     VIRKRRGMPVSLGAVALYLSAVLDLPLYPVNFPTQLVLRAEIHQPNGATKVRFINPWN
                     GEFLTIEMLNKWLEGEMGFGVEVTPDLLRRAEPQELLERVETVFKMALTREGKYEETL
                     RLIEYRLAFSPEDPYEIRDRGMVLASMDCYQAALEDINYFIDQCPEDPSAEILKMEVK
                     GLQQKSQESVVH"
     gene            7665..8519
                     /gene="kdsA"
                     /locus_tag="PXD81_000156"
     CDS             7665..8519
                     /gene="kdsA"
                     /locus_tag="PXD81_000156"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012073169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HDL5536231.1"
                     /translation="MTDKIVKVGNIEVANHKPFTLFGGMNVLESRDMALRVCEQYVEV
                     TQKLNVPYVFKASFDKANRSSIHSYRGPGMEEGLKIFQELKQTFGVNIITDVHEIYQC
                     KPVAEVVDVIQLPAFLARQTDLVEAMARTGAVINVKKPQFLSPGQMGNIVEKIAECGN
                     DQVILCDRGTNFGYDNLVVDMLGFGIMKKVSKGCPVIFDVTHSLQCRDPFGAASGGRR
                     DQVTELARSGMAIGLAGLFLEAHPDPNSAKCDGPSALPLSKLEAFVSQMKAIDELVKS
                     FEEIDTSK"
     gene            8740..9687
                     /locus_tag="PXD81_000157"
     CDS             8740..9687
                     /locus_tag="PXD81_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HDL5536232.1"
                     /translation="MLNIVFLDRTGIPATHDIPRPNFPHTWTEYATTSADQTFERVKE
                     ADIIITSKVLLQRDLLAKLPKLKLIAITATGTNNVDLAAAKELGIAVKNVTGYSSVTV
                     PEHVIGMIYSLKHRLADYHRDLITSDRWATCGQFCYVDYPIHDVRGSTLGIFGKGNIG
                     AEVARLAQAVGMQVIFAEHQGATEIREGYTAFEEVFKQADIISLHCPLTEQTQNLINA
                     ETLALMKPTAYIINTGRGPLIDEQALLEALEQGKIAGAALDVLVKEPPEKDNPLMLAA
                     KRLPNLLITPHVAWASDSAVTTLVNKVTQNMEEFVATGM"
     gene            9785..10957
                     /locus_tag="PXD81_000158"
     CDS             9785..10957
                     /locus_tag="PXD81_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005609574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit"
                     /protein_id="HDL5536233.1"
                     /translation="MNLTPFFIAFRYFRSKSADRFGRLVTNLASLGIVLGVMALVIVL
                     SVMNGLENMQKTNLLSTLPHAIITPQEGHFAKGEKLALPEFAKQSVVINRANVIIQSS
                     QGINAGQLIGVENATDDPLLVAENIPALLPSGEFKVVIGSRLANKLNVTVGDKLRLMI
                     TENSQYTPLGRVPVQRLFEISGVYQSNREASEFTLFANLADVGRLLRISEDQVQGVRL
                     YLQDPFQVTELSQYFDAERYQISDWREQKGEFFQAVKMEKNMMGLLISLIIVVAISNI
                     VTSLSLMVVDKQGEIAILQTQGLTKKQVMQIFVFQGAIVGVIGSIIGGLLGVLIAYNL
                     DQIILLLNPSIHLPTLISGTQIAVIVGVSMLLSLLCTLYPAYRASKIEPAQALRYE"
     gene            10974..11657
                     /gene="lolD"
                     /locus_tag="PXD81_000159"
     CDS             10974..11657
                     /gene="lolD"
                     /locus_tag="PXD81_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HDL5536234.1"
                     /translation="MTELLRCEHISKFYDEGEQKVQVLKEVSFSMNEGELVAIVGSSG
                     SGKSTLLHTLGGLDQPSTGEVWIRGQSLQQLSSDKLALLRNQNLGFVYQFHHLMADFT
                     ALENVMMPMLIGKQNKTEAANRAEKMLQAVGLAHRMTHRPSALSGGERQRVAIARALV
                     NNPALVLADEPTGNLDQKTTESIFELIQQLNQEQNIAFLLVTHDLNLANKLSRSLVMR
                     DGVLSEGSL"
     gene            11654..12919
                     /gene="lolE"
                     /locus_tag="PXD81_000160"
     CDS             11654..12919
                     /gene="lolE"
                     /locus_tag="PXD81_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HDL5536235.1"
                     /translation="MSTPFFISWRYQRGKQKNKLVSLIALFSNIGIALGVAVLIIGLS
                     AMNGFERELNNRVLSVVPHAELVSFQGNQPQPIFDSKKLEALIKKSQNVTASSPFVSF
                     TALIENGAQLKIAQVRGVDPKKQDQASQLSQFIPPEQWQAFNQQFQADEDGLILGAGI
                     AKALEVEAGDEVTLLIPQPTEDGKLAQPLRFNLNVTGILRLDGQLDHSYALIPLNKAQ
                     ELMEYQANEVSGLEISLANPFDVQQLEMPQLNGYTQPLYLNTWIDKFGYMYNDIQLIR
                     TVMYIAMVLVIGVACFNIISTLIMAVKDKQGDIAILRTLGANNGFIRRIFLWYGLISG
                     MKGALFGLILGVILSLNLTAIIKAIEAFFEVKLLSDGVYFVDFLPSELHWQDVVYVLA
                     ATIILSLFASLYPANRAAKLEPAKVLSGH"
     gene            complement(12994..14925)
                     /gene="thrS"
                     /locus_tag="PXD81_000161"
     CDS             complement(12994..14925)
                     /gene="thrS"
                     /locus_tag="PXD81_000161"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011200229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HDL5536236.1"
                     /translation="MPIITLPDGSQRSFDNPVSVMEVAQSIGAGLAKATIAGRVNGER
                     RDASDIISQDSTLEIITAKDEDGLEIIRHSTAHLLGHSIKQLFPDVKMAIGPTIENGF
                     YYDIDLDRSLTQEDLDALEKRMLELAKTNYDVVKRPVSWQEARDTFEKRGEPYKMAIL
                     DENIERTATPALYHHEEYIDMCRGPHVPNMRFCHHFKLQKVAGAYWRGDSKNKMLQRI
                     YGTAWADKKQLAEYLQRLEEAAKRDHRRIGKALDLYHMQEEAPGMVFWHNDGWTIFRE
                     LETFVRTKLKEYDYQEVKGPFMMDRVLWEKTGHWQNYADLMFTTQSENREYAIKPMNC
                     PGHVQIFNQGLKSYRDLPIRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IESEVISCIKMVYDIYSTFGFTNIAVKLSTRPENRIGDDAMWDRAEKGLANALRNNGL
                     EYEIQEGEGAFYGPKIEFALRDCLGREWQCGTVQLDFALPGRLAASYVAEDNGRRTPV
                     MIHRAILGSIERFIGIITEEYAGFFPTWLAPTQAVVMNITDSQADYVQQVVKQLSDAG
                     IRVKADLRNEKVGFKVREHTLRRVPYMLVCGDKEIAEGKVSVRTRKGADLGTYLVSDL
                     VEILKNQIKARELKLLGEE"
     gene            complement(15092..16441)
                     /locus_tag="PXD81_000162"
     CDS             complement(15092..16441)
                     /locus_tag="PXD81_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL5536237.1"
                     /translation="MNSTKFYSLYSGIFFLFFGYGLFLNSAGIKLAEMGITDIIIGLL
                     NAAFFIGAALSTIFAHRIVSSVGHIRSFSVFCAVFAIAALGHIMIDTLWIWGVLRIIL
                     GFCHYSLLLLVESWLSEKTNVDTRGKVLATYNIIFYLAFILGVSLLSLELSSRNIFIL
                     STILVVMSMIPIALTKMVQPDIPARERISIPKLFAISPLALATSFISGMLVNGFFTMA
                     SVFLLKFSFSLSEISLYLMISMIGGFISQMPMAILSNRIGRRNAILACSVIATLTALA
                     GLFAIFQENLTAWGQYIIAFFFGCSLFSLYALSIARANDELPNNMNTVEVSRGLLFCY
                     GVGALVAPPLIGFAMSLSAEYGFYALFGLFSLSLFICAFRTKSIPKAERAEMQYATPI
                     ATASPLEEIEVVEDALIPFDEEAVQEYQAQLEILEQDPQTENENIELDEGAAENSKV"
     gene            complement(16716..17207)
                     /locus_tag="PXD81_000163"
     CDS             complement(16716..17207)
                     /locus_tag="PXD81_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HDL5536238.1"
                     /translation="MFTTQLIQTKRWLEANSITTFWLKTFIGATLLAIFAQISVPMYP
                     VPITGQTLALTVVGFAMGRKAATAAVLLYLFEGAIGLPVFAGGASGFHHFFGPTGGYL
                     LGYIPTAYFLGYYSDKGVLNSFWKSLFVALIASVITFAFGLAQLSLYQKAQYYKRAYI
                     HLF"
     gene            17288..18055
                     /locus_tag="PXD81_000164"
     CDS             17288..18055
                     /locus_tag="PXD81_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--protein ligase"
                     /protein_id="HDL5536239.1"
                     /translation="MVKPVYIYCDEGSSEIGVQSLLLAVKQHLQRPAYLINAKQIIQS
                     KFSDGCLLIMPGGADLPYCKKLNGEGNRRIRQFIEQGGAYLGICAGAYYGCAELNFVG
                     RDYSVNGNRELALFNGIAGIAKGSLPQFTNGQLYDKNIGSKAIVEVTLAEGGKMPFYY
                     HGGCCFEPNSTTVIYNPLAYYPDGSVAMIEGELGRGYYLLSGVHFELQAVVYWQKIVN
                     CCENLDIKTQEEAICSNFNVNYGSQIWQHLKQRLNQA"
     gene            complement(18046..18840)
                     /locus_tag="PXD81_000165"
     CDS             complement(18046..18840)
                     /locus_tag="PXD81_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HDL5536240.1"
                     /translation="MRFFDTHTHLDYLAESLNLSIPELVENAKQHQVECILIVAVFAK
                     NFAKVTACAKQKPENLVYGLGLHPLYIQQHQQQDLILLENLLSQQDPQCTAIAEIGLE
                     KAVSEVSTPELWKKQCEFLEAQLSLAKKFNLPVNLHSRKSHDQLFVFLKKAQLTKTGV
                     VHGFAGSYEQAKRFVDLGYKIGVGGTITYERANKTREAIRKLPLESLLLETDSPDMPV
                     FGFQGQPNRPERLLVTFNSLCELRTESPNLIAETIWQNSERLFFYA"
     gene            complement(18901..20487)
                     /locus_tag="PXD81_000166"
     CDS             complement(18901..20487)
                     /locus_tag="PXD81_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005667512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein A"
                     /protein_id="HDL5536241.1"
                     /translation="MLWFFFCVAVLVLGYFTYGKIIEKIFVINPKRATPAYAMNDGVD
                     YMPMSKTKIWLIQLLNIAGTGPIFGPILGALYGPVAMLWIVIGCIFAGAVHDYFCGML
                     SIRNGGATMPNLAGKFLGRPVKLFINVMALILLLLVGVVFVASPAQLLSTITMDIAGA
                     TQSAMELGDVEAIKESVASGGVTVWGMDKATVISVWTLIIFAYYILATLLPVDKIIGR
                     IYPFFGALLLFMSVGMVYGLVSAHLSATDPIDFFRTVNADGQGLTWEKFTQNFQVKGD
                     VPIWPLLFLTISCGALSGFHATQTPLMARCAQNEKEGRFIFYGAMITEGVIALVWCAV
                     GLSFYENPQALQDAISAGSPSKVVYDSSLHFLGFIGGIFAVLGVVVLPITSGDTAFRA
                     ARLQLAEIFGLEQRTLVKRLYIAVPLFVLGFIVSKVDFSVLWRYFTWANQMTAMVMLW
                     TAAGYLYRYKKFHWVCSIPAWFITTVCATYLANNKIGFGLDYELSVYIGFAITVVCVI
                     CFFAFLKPSEARDEDAYVTN"
     gene            complement(20673..22526)
                     /gene="dxs"
                     /locus_tag="PXD81_000167"
     CDS             complement(20673..22526)
                     /gene="dxs"
                     /locus_tag="PXD81_000167"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HDL5536242.1"
                     /translation="MEKKYPLLSEINSPEDLRLLSKDQLQTVVDELRAYLLESVSQTS
                     GHLASGLGVVELTVALHYVYQTPFDQLIWDVGHQAYPHKILTGRRDQMHTIRQKDGIH
                     PFPWREESPYDVLSVGHSSTSISAGLGIAVAAEKENAGRKTVCVIGDGAITAGMAFEA
                     LNHAGALHTDMLVILNDNEMSISENVGALNNHLARIFSGSLYTSVRDGSKKILDKVPT
                     IKNFMKKSEEHMKGVIFSPESTLFEELGFNYIGPIDGHNIDELVKTLSNMRELKGPQF
                     LHIRTKKGKGYTPAENDPIGYHGVPKFDPTAAELPKSKAAPTYSNIFGDWLCEMAERD
                     PKIIGITPAMREGSGMVEFSKRFPEQYFDVAIAEQHAVTFGAGLAIAGYKPVVAIYSS
                     FLQRAYDQLIHDVAIQNLPVIFAIDRAGIVGADGQTHQGAFDLSFMRCIPNMTIMAPS
                     DENEMRKMLYTAYTMNTPSAIRYPRGNAKGVKLEPMQRLEVGKGNIIREGKKVAILNF
                     GALLDEAKIVAEKHNYTLVDMRFIKPLDEALLQKVADSHELLVTLEENAIQGGAGSFV
                     NEYLQNIGKIKPLVMLGIPDFFVPQSTQAEAYADLGLDAQGIEAKIQKFDI"
     gene            complement(22571..23383)
                     /gene="suhB"
                     /locus_tag="PXD81_000168"
     CDS             complement(22571..23383)
                     /gene="suhB"
                     /locus_tag="PXD81_000168"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012621556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HDL5536243.1"
                     /translation="MNPMLNIAIRAARKAGNIIAKGYEQSPQDTQVLQKNTNDYVTEI
                     DKAAEAAIIDVIRKSYPEHSIVTEESGVLNGSNENVQWVIDPLDGTTNFVKRLPHFAV
                     SIAVRENGRTTVGVVYDPIRNELFTAVRGEGAKLNEFRLRIDAERRDLKGTVIATGFP
                     FKVIRHRDAHLNMLEGLMNNGVADFRRTGSAALDLAYVAAGRVDGFFEIGLKPWDFAA
                     GELIAREAGAIVTNFVGGTDYLKTGNLVVGNGRVVKEILNSIQPTLTEELKA"
     gene            23564..24598
                     /locus_tag="PXD81_000169"
     CDS             23564..24598
                     /locus_tag="PXD81_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA methyltransferase"
                     /protein_id="HDL5536244.1"
                     /translation="MQFKENNQPRFKHREERRSDECSPRFSDKGKMRGNEGKSDRPHK
                     LHFKRDEAYSRSEQIYPAEGKKASGEGSVQIWVKGGNSSVKEKKNGPLSPRAPEKIKK
                     NRLEEMKVYGENACISLFQQRPESIVRLWATVEGAKKLGDLMSYLAQHKKAYHVIDRE
                     EMERVTGTEHHGDMCLLVKKTASFVLEGYLQVPKKQDCLVLLDGVNNAQNVGGIIRTC
                     AFYGVKGIITENVDSLNSSAAARVAEGGLEFVRALETKNKHIALVQLRQAGYQIVHLT
                     RHKQAPSLTKIKLAEKVVFVLSEIPASDVAYAEDTTIQLSFNNPLNSGLNVAVNAGIL
                     LNQWYQTHIL"
     gene            24608..25492
                     /locus_tag="PXD81_000170"
     CDS             24608..25492
                     /locus_tag="PXD81_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HDL5536245.1"
                     /translation="MNQQKPFEVIAIVGTPRHDSAFETHLAVYNWLKDRDYQILVETE
                     VATQLQLKESYSLDQIGQRAELVIVIGGDGNMLGIARALAKYRVPLIGINRGNLGFLT
                     DIAPQTAFEQLYSCLVKNEYVIEERFLLEAFIERNGKIIAANNALNEIVVHPTQVARI
                     IEFEVYIDGKFAFSQRADGLIVATPTGSTAYSLSAGGPILTPNMNAMALVPMHPHTLS
                     SRPLVVDGDSQISLRFAKYNTPDLEVICDGQVNMHFTPEDRVLVRKSPDKLQLLHLKD
                     YSYFTVLGSKLGWASKLF"
     gene            25631..26608
                     /locus_tag="PXD81_000171"
     CDS             25631..26608
                     /locus_tag="PXD81_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1852 domain-containing protein"
                     /protein_id="HDL5536246.1"
                     /translation="MTTNFKCQVHTIEFNEDYQPEQSTRLTTNFANLARGENRKQNLQ
                     NAIAMINNRFNDLANWDNPNADRYSVKLDIVSLDIDVEGKGEFFPAIEWLKTYVIDYK
                     TGKTIDGIIGNNFSSFVRDYDFSVLLLDHNKDKPQFSVPEGYGELHGKLFKHLLASPE
                     YTAKYKKPPVICLSVATTRTYTRTTNVHPILGVEYLQDSYSLTDAYFAKMGLKVRYFM
                     PPKSVAPYAFYFDENADLLNDYTNLELISTIATMEAFQKIYRPEIYNANSVAGQVYKG
                     SLTYQDYSVTKVEYDREERTQLAIKQGKYTEEHFIKRYQAILEAWATEE"
     gene            26696..27727
                     /locus_tag="PXD81_000172"
     CDS             26696..27727
                     /locus_tag="PXD81_000172"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HDL5536247.1"
                     /translation="MSNILLPTSTAGSLPKPSWLAEPEKLWSPWKFEGEELLQAKRDA
                     LLVSLAEQIAAGIDIVSDGEQTRQHFVTTFIEHLEGVDFSKREIVRIRNRYDASVPSV
                     VGAVSRPKSVFVDDAKFLRAHTDKPIKWALPGPMTMIDTLYDGHYKSREKLAWEFAKI
                     LNEEAKELEAAGVDIIQFDEPAFNVFFDELNDWGVAALERAAEGLKCETAVHICYGYG
                     IKANTDWKQTLGNEWRQYEESFPKLQQSKIDIISLECQNSRVPMDLIELVRGKKVMLG
                     AIDVATNIIETPEQVADTLRKALQFVDADKLYPSTNCGMAPLSRQVAQGKLQALSQGA
                     AILRAEFAK"
     gene            27815..28036
                     /locus_tag="PXD81_000173"
     CDS             27815..28036
                     /locus_tag="PXD81_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5536248.1"
                     /translation="MLNLFKKPIKKSSFEAWAKLMDDVAKVAILAIPVILYGEYSLLM
                     KFTHSITLFAVVYFLLATARLFRETAEEM"
     gene            28312..29574
                     /locus_tag="PXD81_000174"
     CDS             28312..29574
                     /locus_tag="PXD81_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000919159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HDL5536249.1"
                     /translation="MLKRDMNIADYDAVLWQAIQDEDRRQEEHIELIASENYASPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKALFGADYANVQPHSGSQAN
                     AAVYMALLNPGDTILGMSLAHGGHLTHGASVSFSGKIYKAEQYGITDEGLIDYEALRK
                     QAQEVKPKMIVAGFSAYSQVMDWAKMREIADEVGAYLFVDMAHVAGLIAAGVYPNPLP
                     HAHVVTTTTHKTLAGPRGGLILSASGDEEMYKKLQSAVFPAGQGGPLMHVIAAKAVCF
                     KEALEPEFKAYQQQVVKNAKAMVEVFKQRGFDVVSNGTENHLFLVSFVKQGLTGKAAD
                     AALGAANITVNKNSVPNDPQKPFVTSGIRVGTPSVTRRGFKEAEVTALAGWMCDVLDS
                     IGKDNHEQVIAETKAKVLDICKRLPVYA"
     gene            29732..30568
                     /locus_tag="PXD81_000175"
     CDS             29732..30568
                     /locus_tag="PXD81_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChaN family lipoprotein"
                     /protein_id="HDL5536250.1"
                     /translation="MLPRISSFLASLFMAFFLLGCSVSTQSLNEQNVDSIGQIIDLNT
                     NQTISFTELLSRLSSQDYVLVGEEHTQLIHHQIELYLFNQLTKKAKLHLIALEMLNVD
                     QQPAIDRIQFNRPVNLSATDLREAIQWQKWDWKMYQDLVVESLGSNSRVIATNLTDKE
                     VEILLNGAEPLKGSVSTSSEVKQKIAQLLLSMNHGMPYPMENMVAVQQFRDRRMAEKL
                     VKNALSTSLLIAGNHHVRKDLGVPLHIAEYDRTKKVAVLMLKTEREEITSSQADYLWV
                     TQ"
     gene            30622..32779
                     /locus_tag="PXD81_000176"
                     /pseudo
     CDS             30622..32779
                     /locus_tag="PXD81_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484758.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
     gene            32886..33524
                     /gene="purN"
                     /locus_tag="PXD81_000177"
     CDS             32886..33524
                     /gene="purN"
                     /locus_tag="PXD81_000177"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005693938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HDL5536251.1"
                     /translation="MKKFVVLISGNGSNLQAMIDAQKSADTSGQICGVICNKADAYGL
                     IRAKQAGIPTFVFSRKDYQSNVEMDLAIAEQIEQLGAELIVLAGYMKILTPEFTQHFA
                     GKILNIHPSLLPKYPGLNTYQRAIEAGESEHGTTIHFVNEEVDAGAVVLQAKVPIYPE
                     DEIEDVMARVVEQEHRYYPLVIEWFCSGRLVSQHGKAYLDGKLLPACGYAED"
     gene            complement(33545..34351)
                     /locus_tag="PXD81_000178"
     CDS             complement(33545..34351)
                     /locus_tag="PXD81_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL5536252.1"
                     /translation="MSNLLTCQNLSIVHQQKKLLEIQEIAFEQGKFTAIIGPNGAGKT
                     TLLKALSGNNAQTNSAVFYQHQRLTDLNGYALAQKRAMLSQQSQIAFPLKVEELVQLG
                     REPYRQSEFAKFNEKITAWAMETMSLTALKERSSIQLSGGEQHRAHIARVLAQLVPEP
                     NADLSGSWLLLDEPTNHLDIHHQYQLFSLLQKLKQQGLTIIAILHDPALAINQADHIL
                     MLKNGEKFAEMSAKAIAESCLLDELYEMKMGCRYCPHSGQYYLCPALAES"
     gene            complement(34351..35337)
                     /locus_tag="PXD81_000179"
     CDS             complement(34351..35337)
                     /locus_tag="PXD81_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HDL5536253.1"
                     /translation="MKLHLTLIFTLIISLVICTHLGAMSLPLSAWHCNNAECESAAYI
                     LWQIRLPRLLGALFIGAALAISGATMQGLFRNPLVDPGIIGVSSGAALAAALYIVVIQ
                     PLLPESISYYALPIAAFLGGWLVTFMLYLLSQRNGQLHIAIMLLIGIALAAFSGALTG
                     ILIYVADDNQLRSLTFWSMGSLAGMNWQMVAMMAAVVFICSPLIWREHRALNALTLGE
                     EDAQYIGFDIAKVKRRLVFYVALLTGTSVACVGAIGFVGLVIPHLMRLLGGSNHRHLL
                     LNSLVCGAILLMIADTLARTLAAPAEIPIGIFTALFGAPFLAVMVWKSGKHI"
     gene            complement(35348..36214)
                     /locus_tag="PXD81_000180"
     CDS             complement(35348..36214)
                     /locus_tag="PXD81_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL5536254.1"
                     /translation="MEYKMRTLLFSFIALSMCLNTTKAAEKIVSTAGYASEIVAALGK
                     ADKLVGVDTTSVKPQTIMEKKPKIGYRRQLSAEGILSLTPDLIILAPDAGPNAVIEQI
                     KASGIPLLHLGEKPSLAGIREDIQQIAKAIGAESEAKQLTETILADEKKLADIRSQHS
                     TDKEVLVLIDTASQGIFGLGKKSVGDHFLTLLNLNNSFNAEGNKPLANEALAASNADV
                     ILLASRNQAKESATISKLAESSPQYAQLTNTQAGKKGCVFAINILDALGFGPYTAQYA
                     HQILTTIQPCLK"
BASE COUNT        11032 a         7216 c         7836 g        10155 t
ORIGIN      
        1 ttttttagga ttttcgatca agatcacaaa aataaatttg cgagcaaggc aatgcccttg
       61 gttcctcaaa gggattaaga gcattaatca acataatttt ttcatattga tgcaaatctt
      121 gcttggctat tttggctaaa acgactttcc cttcatcaag caatttacta agctgagtgc
      181 ctttgagcaa ataatgttgc gggctatacc agattccttg ttttaggaac agtaaattgc
      241 cgatggagca atcactgacg tagccattat tgagaataat cacctcttca ttttgtaaaa
      301 tagggagcaa atcaaaccgc ttgcggtcgc taaatttgag gtgataatcc caattttcca
      361 cctcaaccaa gcgaaaacgt tctacttttt tgggcgaata gataaaaaat ttcagctcaa
      421 attctgttgc gttgtaatct attcggcaac gtactttgcc ccgttgagaa gcctctggca
      481 caactaaaat atcagcaagg ctaaaacgag gttcacagcc aaaatattct tgtgttgctt
      541 gctcaaaacg ttgttgatga tactgtaagt tctgaacttt gccgtcaata atcgaaaggg
      601 tttcaaatag cggaaatcgg cacatagact ttctctaaca gttcattata ttcatcagtt
      661 aacacacttt gtgaggtaat gccaccacca ctatgaaaca caagcccgtt tgaggtttga
      721 gcaatgtaac gaattgccac cgcactttct aagctctcgc catcaaaata accaaaaata
      781 ccggtgtagt aaccccgttt tcctaattct gcttgctgaa taatcgaaac cgttttctct
      841 ttcggtgcac cgctaatgga gccggcaggt agtaattttg ccaatatcga accgatattt
      901 tcttgccaat tttcacctaa ttcgccacag atctcggaac ttgtctgcaa aattgcccct
      961 cgatgggttt ctactttttc cacataacgg tatttagtaa cttcaatatt tttcgccacc
     1021 aacgccaagt catttcggat caaatccaca atggtattat gttcggtaaa ctctttttcc
     1081 gaagccatta atttctgctc ggcattctcc tcatttgcat taattgtgcc tttcatcggg
     1141 taggaaaaaa tgcggttatt ttcaattcgc acaaagcatt caggggaaaa acagacgaac
     1201 ctgccttgca aataacattt atattttgct ttgctggcgg caaaaatttc cgccaatgaa
     1261 taattggttt caatggcagt cgggtagctc aaattcagca agtagctatt gccggcttga
     1321 atttcctttt tcactaattc aaacccggtt tggtattcgc taaacggaat cggatttgcc
     1381 gataattcaa aagctttagt cggtttttca accggctttt cgagattcga tttgcaaaaa
     1441 aattcaaaaa aaagaccgct tgcgtgagcc tgttctagcg gaaaaataag tggcttttgc
     1501 tgctcaaaat caattaaaaa gaaaaaaggg cgttttgctt gcccgtagtg attggcttgg
     1561 tgaataaaat cttgcataga aaacacaatg gctgaaattg gctgaatcat caaaagctct
     1621 gttataatgc aaagcaatag atttgaaaag cattttttaa aaaaggtttt accgatgaac
     1681 aacaccattc attttcaaga aattgggatc tacatcgccc ttgccgtttg tgtgctggta
     1741 ggcgtttatc tctttttctt gaaatcccgt tatcagcgaa atgcctacga attaagccaa
     1801 gatcttggca aaatcaccga aaattttgaa caaattcagc aaaaatatga ggtgttggct
     1861 caagaaaaaa atcagcttga gcagtgggca attcaacaac agaccaaata tgaagcagtg
     1921 agtgaacgtt taaacgagcg agatgggcaa ttgcaacgat tccaacaaaa aatcgaactt
     1981 gccgaacagc aagaaaacca gctggaacgc tatattaatg agctaaaaga acgtgtgggt
     2041 tcttcccaag caaaggcaga aagtttggaa gaacagctac aatttagcca aagttcactc
     2101 tcaactaaag agcgggaaaa tcaagcgtta tttagccgtt taaacgaggt acaaaacgag
     2161 ctgaccgaac tgcgtactac gctttcggaa aaacaggcaa attttgaagc acaacaacgc
     2221 aattttattg aggtaaaaca acagcttaat gtggaatttc aacatttggc acaacaaatt
     2281 cttgacgaaa aaagcaagcg ttttagcgaa accaatcaaa gctcgttaga agctctactg
     2341 aagccgttca aagagcaaat tgaaggcttt caaaaacggg tgaatgaggt acatagtgag
     2401 tcgctcaaag gctcggcaaa tttagaggct gaaattaagc gggtgctaca aattggcgtt
     2461 tcaatgtcgg aagaggcaca aaatctggca acggcattaa aaggcaataa taaaattgcc
     2521 ggcaactggg gtgaagtaca gcttgaaagt gccttacaat ctgccggttt actcgcaggc
     2581 gagcattacc aagcccaaga gagttttcgt gatgaagagg ggcgacgttt tgcccccgat
     2641 tttgtggtgc atttgccgga tcaaaaacat ctgattattg acagtaaagt ctcgttagta
     2701 gcgtacgatc aagcggtcag atcagaagaa aattttgcaa tttctcaagc cttagacgaa
     2761 cattgccgtt cgctacgcaa tcatattgag ggattgtcta agaagaacta cagtgcatta
     2821 ttaggcatta aaagcccgga ttttgtactg atgtttattc cgatagaacc ggcttatatt
     2881 gaagcgatga agcacgatcc gcaactgttt aactttggct atgaacgcaa tgtgattttg
     2941 gtttctcaca ccaccttaat gccgatttta cgcacggtcg ccaatttatg gcggattgaa
     3001 cgaggcaaca gcgaggctcg tgaaatcagc gaaaaagccg gcgaaattta caaccaagtg
     3061 tgtgcggttg ccgatcggtt agctaaatta ggtaacacgc taaataccgc aaataatcaa
     3121 tacaatcaaa ccgttacctc gttagtggga cggcaaggct tactgggcaa agtggaacga
     3181 ttccaacatc tttcaagcaa agcaagccaa attgtgccgc aggttgagct gattgaaaat
     3241 gaggtggata ctattaaatt ggaaatggta tcgagagaat tattggaaga atagtcaaat
     3301 ctctttattc cctacaaaaa tatggggtga tttgaaatac taaagaaaat aataaaatgc
     3361 taaaaaaact attaatcatc gacaaccacg actcttttac cttcaacttg gtggatttac
     3421 tccgtaagct tgatgtgccg gcgaaagtgg tgctggttga agagcttgac ctcaatgagg
     3481 tggagcattt tagccatatt ttgatttcgc ccggccctga tgtgccgaga gcctatctac
     3541 aaacctttgc gatgttggag cgttatcatc aaaccaaatc aattctaggc gtttgcttgg
     3601 ggcatcaaac gatttgcgag ttttttggcg gcaagttgta taacctaaac gatgttagac
     3661 acgggcagca acgcacgctc actcaaattg agcctaaccc catatttaaa ggtttgccta
     3721 agcagttgaa agttgggttg tatcactctt ggggcatttt gcaaaattcg ttgcaaaata
     3781 caccgcttgt accaaccgca gtttgcgatc aaaatgtgtt aatggcgttt aaacaccaac
     3841 atttgccgat ttttggcgtg cagtttcacc cggaatcttt tatcactgaa tttggtcaac
     3901 aaatgttggc aaattggctt gagattacac aagattttta gcgtgaaatc cgctataatc
     3961 cgtcagttat ttaccctttt aaaatctgta ggggcgaaaa atttttcgcc ttacttaata
     4021 tgcaagagat aatttaaatg aaagattcga ttatcaataa attagaaagt ttaagtgaac
     4081 gccacgaaga actacaggcg ttacttggcg atgcctcaat tatttccgat caagacaaat
     4141 tccgtgctta ttccaaagaa tattcgcaat tagaagaagt ggttggcacc tttaaccgct
     4201 ggaaaaagct gaacaatgat attgaagagg ctcaattatt actcgatgat cctgatatga
     4261 aggatatggc agcggaagaa attgcggaaa ataaagccga aatcgagaat cttgagcaac
     4321 atttacaaat tttattattg ccgaaagatc cgaatgatga atacaacgcc ttccttgaaa
     4381 tccgtgccgg aacgggcggt gacgaggctg gtatttttgc cggtgattta taccgtatgt
     4441 acagccgtta ttgcgaaagt aaacgctggc gaattgaaga aatgtcggca aatgagagtg
     4501 aacaaggcgg atataaagag atcatcgtta aaatttcagg cgaaggcgtt tacggtcagc
     4561 ttaaatttga atcgggcgga caccgtgtac aacgtgtacc gaaaaccgaa tcacaagggc
     4621 gtattcacac ctctgcttgc acggtagcgg taatgccgga gttgccggaa agcgagatgc
     4681 cggaaatcaa tccatctgat ctgcgaattg acacctaccg ctcatcgggt gcaggcggtc
     4741 agcacgttaa taccaccgac tctgcagtgc ggattactca tatcccgacc gggattgtgg
     4801 tcgagtgtca agatgagcgt tcacagcaca aaaataaagc caaagcgctg gcagtgttag
     4861 cctctcggat tgtacaagtt gagcaagaac gccaagccgc agagcaagcg gatacccgcc
     4921 gtaacttact tggctcaggc gaccgttcgg ataaaatccg cacctacaac tacccgcaag
     4981 ggcgtgtgac tgatcaccgt attaacctca cggtttatcg tttagacgaa gtgatgaacg
     5041 gcaaaattga tgaattgata caaccgatta tcactgaata tcaagcggat cagttagcgg
     5101 cgttgtcgga gcaaaattaa ttttattgta agggtacact gtgtgtgcct gccaaatata
     5161 aatttgcaaa attttgacta tttttgatcg cttgtagttt ctaaaaagtt taaaaatttg
     5221 aggaaaacaa aatgagaaag ttaggtttat tagcagtatt tactttagta ttatcagcct
     5281 gttcaaattc aacaaaaatt gaacaaacca ttctttccaa gcaggatatc gtaggctctt
     5341 gggtatgcgc tattaaatac gatgatctaa aagtaagcac tttagatttt tctgaattta
     5401 aagctaatgg cgaaatgacg aatttcggtg atatttctga tgagaatttt gagcccatta
     5461 aatttactta tgtaacagga gataaaggga agtgggactt aaaaggaaat cgattagtga
     5521 ttgattatga tctttctgaa cgtaaagtaa aaagaacaac accaaaaaaa tttttagagt
     5581 tcttgaaaca ggaagagcgt aaagcctcaa aaacagctaa aaaactcctt aaatatgaac
     5641 aagggatttt caatattctg agtgatcaga aaaatactga aaacagtaaa ataatattag
     5701 atattttgaa gtttggtcat tcaagaatgg cgattcagca aaatatggga gacaaagttt
     5761 attcaggtgg ttgtgtaaca gctgataaag cagaagagta tttagatatc ctaaaaaata
     5821 gaaattctaa ataatggatt accgagagtg gctcactttt gctgagcaaa aattacttga
     5881 aaatagcggg gcagatccat acctaaacgc aaaatttgat gcaaatttgc tattgcaagc
     5941 agtcacaaaa cgttcaaaat cggccatttt tgctttttcg gaaaccctac tttctgaaag
     6001 cgagcttgca gaattagcgg aaaaattagc tcggcgtgcc aaaggtgaac caatggcata
     6061 tattttaggc tatcgggaat tttggtcgtt gccgttaaag gtttcaccgg caacgctgat
     6121 tcctcgccct gatacggaac gactggttga gttggcgttg gaatatgcta acaagcggtt
     6181 aggaaatcag aaaatgttgc aaattctgga tctcggtacc ggcacggggg cgattgcgtt
     6241 ggcactggcg agtgaattag gcgagaaagc ccaaattatc ggggtggatt tccaaaatga
     6301 ggcggtgcaa ctggcagaag aaaatcgccg aaacttaggc ttaaatcaag ttacattttt
     6361 acaaagcgat tggttcagtt cgcttgcaaa tcaacaattt gatttaattg tctcaaatcc
     6421 gccttatatt gatgaatcgg acgaaaattt gacggtggga gacgtacgtt ttgaaccttt
     6481 aaccgcatta gttgccgata ataaggggtt gagcgatttg caaaaaatca tcgaaaatgc
     6541 accgcttcac ttaaaaccgc aaggggcgtt aatgctggaa cacggctgga aacaaggtaa
     6601 cttggttcga gatttatttg atttacactt gtgggaacag gtagaaactg ctcaagatta
     6661 tgccggcaat gaccgagtaa ccaaagcgat tttaaaataa ggcagaaaat ggacgatatt
     6721 attgaagaat tgttaaaaga gttggaagat ccgaaagtaa ccatcagcga aaccgagctg
     6781 aaaaagtttc tttatcgtga aattttacga ttaactacgc tcattgataa gaatgtaagt
     6841 gagcaacaag tgtttggtca gatgtctgcg ttggtgaaaa aagctcgcta taacgtgaat
     6901 ggcgaaggcg atgaagagag aattaaccaa ttacttcatt tagtctatcg tgaatggggc
     6961 tttcattgct actatgaaga ctatttccac accgaaaatt tgttgctaaa tcaagtaatt
     7021 cgcaaacggc gtggaatgcc ggtttcactt ggggcggtgg cgctttattt atccgccgta
     7081 ttggatttgc cgctttaccc ggttaatttt ccgactcagc ttgtgttacg ggctgaaata
     7141 caccagccga acggggcaac caaagtgcgt tttatcaacc cgtggaacgg tgaatttctc
     7201 accatcgaaa tgctgaataa atggctggaa ggagaaatgg gctttggcgt agaagttacc
     7261 cctgatttgt taagacgagc agaaccacaa gagttgctgg aaagggtcga aactgtgttt
     7321 aaaatggcac ttacccgtga aggcaaatat gaagaaacct tacgcttgat tgaataccgc
     7381 ttggcattta gcccggaaga tccgtacgaa atcagagacc gaggaatggt actggcaagt
     7441 atggattgct accaagcagc attagaagat attaattatt tcattgacca atgccctgaa
     7501 gatccttctg ccgaaatttt aaaaatggaa gtgaaaggac tgcaacaaaa aagccaagaa
     7561 agtgtggtgc attaagctga gaagtagccc caaaggggct gagctatgat taacctagta
     7621 cggctctgcc gtgctaggca gaaaataaga aacaaggact ccctatgaca gataaaattg
     7681 taaaagttgg caatattgaa gtggctaatc acaaaccgtt tacgcttttt ggtggtatga
     7741 atgtgctgga aagccgtgat atggcactta gagtatgtga gcaatatgtt gaagtcacgc
     7801 aaaaactaaa tgtgccttat gtgttcaaag cctcatttga caaagcaaac cgttcttcaa
     7861 tccactctta ccgtggaccg ggaatggaag aaggcttaaa gattttccaa gaattaaaac
     7921 aaacctttgg cgtgaacatc attaccgatg ttcacgaaat ttaccaatgt aaacctgtgg
     7981 cagaagtagt tgatgtgatt caactaccag catttttggc tcgccaaacc gatttagtgg
     8041 aagcaatggc tcgtaccggt gcggttatta acgttaaaaa gccgcaattt ttaagcccgg
     8101 gtcaaatggg gaatatcgta gaaaagattg ctgaatgcgg caacgatcaa gtgattttgt
     8161 gcgaccgtgg cactaacttt ggctacgata atctagttgt ggatatgctt ggtttcggca
     8221 ttatgaaaaa agtgtcgaaa ggttgcccgg tgatttttga tgtgactcac tcattacaat
     8281 gccgtgatcc attcggtgcg gcttccggtg gtcgcagaga tcaagtaacc gaactggcac
     8341 gctccggtat ggcgattggt ttagccggct tgttcttaga agctcacccg gatccaaact
     8401 cggcgaaatg tgatggtcca tctgcattac cgctgtcaaa attagaagcc tttgtttcac
     8461 agatgaaagc gattgatgag ttggttaaga gctttgaaga aattgatact tccaaataaa
     8521 ctttctccgc attaaatcct ttcctacttt ctttgcttcg ccaaagaaag taggcaaaga
     8581 aaggcgaccc tacttcgcta cttttcttcg cttagtgtga atgggcttaa cgaaaaattt
     8641 acattcgctt cgctcagagt aaattttcct acccattaaa acacgctcag gtagctcaga
     8701 agggagttag aaaggtaaaa gacagaatag gagaaaaaaa tgctaaatat tgtttttctt
     8761 gaccgtaccg gtattcctgc cactcacgat attcctcgtc cgaattttcc acatacttgg
     8821 acggaatatg ccaccacctc tgccgatcaa acttttgagc gggtaaaaga agcggatatt
     8881 atcattacca gcaaagtgtt actccagcgt gacttgctgg ctaaattacc aaagctgaaa
     8941 ttaattgcga tcacagcaac cggcacgaac aatgtggact tagcggcagc aaaagagtta
     9001 gggattgcag tgaaaaatgt aaccggctat tccagcgtta ccgtgccgga acacgtcatt
     9061 ggtatgattt actccctgaa acatcgttta gccgattatc accgtgattt aattacgtct
     9121 gaccgctggg caacttgcgg ccaattttgc tatgtagatt acccgatcca cgatgtgcga
     9181 ggctcaacct taggcatttt cggtaaaggg aacatcgggg ctgaagtggc acggctggca
     9241 caagcggtcg gaatgcaggt tatttttgca gaacatcaag gcgcaactga aattcgagaa
     9301 ggctacaccg cctttgaaga ggtattcaaa caagcagata ttatttcact acactgccct
     9361 ttaaccgagc agactcaaaa cctgattaat gcagaaacct tagccttaat gaagccgacc
     9421 gcttatatta tcaatacggg tcgaggccca ctgattgatg aacaggcatt gcttgaggca
     9481 ttagagcaag gcaagattgc cggcgctgcg ttagatgttt tagtgaaaga gccgcctgaa
     9541 aaagacaacc ctttaatgtt agccgcaaaa cgtttaccga atttactgat taccccacac
     9601 gttgcgtggg caagcgattc tgcggttaca acactagtga ataaagtgac acaaaatatg
     9661 gaagagtttg tagcaacggg tatgtagctt gaagtagggg ctggtcctgt gccagcccta
     9721 atattaatag aatgagaggg cgggtataaa acccgcccct accaatatca ttttagaaaa
     9781 acagatgaat ttaacccctt tttttattgc ttttcgctat tttagatcga aaagtgctga
     9841 ccgtttcggg cggttagtca ccaatcttgc cagccttggc attgtgctgg gcgtaatggc
     9901 attggtgatt gtgctgtcgg ttatgaacgg tttggaaaat atgcagaaaa ccaatctgct
     9961 atccaccttg ccgcacgcta tcatcacccc tcaagagggg cattttgcca aaggtgaaaa
    10021 actggctcta cctgaatttg ccaagcaaag tgtggtgatt aaccgtgcga atgtgattat
    10081 ccaaagttca caaggcatta acgccggtca gttaatcggc gtggaaaatg ccacagacga
    10141 tccgttgtta gtggcggaaa atatccccgc attattgcct agcggtgaat ttaaggtagt
    10201 aattggctct cgtttagcca ataaattgaa cgttacggta ggcgataagc tccgcttaat
    10261 gatcactgaa aacagccaat atacgccact tgggcgagtg ccggtacagc gtttgtttga
    10321 aatttcgggc gtttatcaat ctaatcgtga agcgagcgaa tttaccctct ttgctaattt
    10381 agctgatgtc gggcgtttgc ttcgcatcag cgaagatcaa gtacaaggcg tgcgtttata
    10441 tttgcaagat cctttccaag ttaccgaact tagccaatat ttcgatgcag aacgctacca
    10501 aatcagtgat tggcgtgagc aaaaaggcga atttttccaa gcggtgaaaa tggaaaaaaa
    10561 tatgatgggc ttactcatca gcctgattat tgtggttgca atttccaaca ttgtgacttc
    10621 actcagtttg atggtggtag acaaacaggg cgaaattgca attttacaaa cccaaggctt
    10681 aaccaaaaaa caagtgatgc agatttttgt gttccaaggg gcgattgtcg gagtaattgg
    10741 ctcgattatt ggtggtttgc tcggcgtatt gatcgcctac aatttagatc agattatcct
    10801 gctattaaac ccgagcatcc atctgcctac cctaatttca ggcacgcaaa ttgcggtcat
    10861 tgtaggggtg tcgatgttgc tttcactcct ttgcacgctt tacccggcat accgagcctc
    10921 aaaaattgag ccggcacagg cattgagata tgaataataa gaacggaaat aaaatgacag
    10981 aattacttcg ctgtgaacat atcagcaaat tttacgatga gggcgaacag aaagttcagg
    11041 tgctaaaaga ggtgtcattt tcgatgaacg aaggtgagct ggtagcgatt gtcggtagtt
    11101 caggttccgg caaaagcacc ctattgcaca cattaggcgg gctagatcag ccaagcaccg
    11161 gtgaagtctg gattcgaggg caatctctgc aacagctcag ttcagacaaa ttagcgttat
    11221 tgcgtaacca aaacttaggc tttgtgtacc aatttcatca cttaatggca gactttaccg
    11281 cccttgaaaa tgtgatgatg ccgatgctga tcggcaaaca gaacaaaact gaagctgcca
    11341 atcgtgccga gaaaatgcta caagcggtcg gattagctca tcgaatgaca catcgcccat
    11401 cagcactttc aggcggcgaa cgccaacggg tggcgattgc ccgagcgttg gttaataatc
    11461 cggctctggt acttgccgat gaaccgaccg gtaatttaga ccaaaaaacc actgaaagca
    11521 tttttgagct gattcaacag ctcaaccaag agcagaacat tgcgttttta ttagtaacgc
    11581 atgatttaaa tctagcgaat aaactttcac gtagtcttgt aatgcgagat ggcgtgttaa
    11641 gcgaggggag cttatgagta cgccgttttt cattagctgg cgttaccaac ggggcaaaca
    11701 gaaaaacaaa ctggtttcac tgattgctct cttttccaac atcggtattg cccttggggt
    11761 tgcggtatta attatcgggc taagtgcaat gaacggcttt gagcgagagc tgaataatcg
    11821 tgtgctttcg gttgtgccac acgctgaact ggtttcgttt caaggcaatc agcctcaacc
    11881 aatttttgat agcaaaaaat tagaggcatt gataaaaaaa tcgcaaaatg tgaccgctag
    11941 ttcgcctttt gtcagcttta ccgccttaat tgaaaatggg gctcagctta aaatcgccca
    12001 ggtgcgaggg gttgatccta aaaagcaaga tcaggccagc caattaagcc aatttattcc
    12061 acccgagcaa tggcaagcgt tcaatcagca atttcaagcc gatgaagatg gcttgatttt
    12121 aggagccggt attgccaaag cgttagaagt tgaggcagga gacgaagtta cgcttttaat
    12181 tcctcagcca acagaagacg gcaaattagc ccagcccttg cgttttaacc taaacgtaac
    12241 cggcattttg cggctagatg ggcagctgga tcacagttat gccttaattc cactgaataa
    12301 agctcaagag ctgatggaat accaagcgaa tgaagtgtca gggcttgaaa tcagccttgc
    12361 caatccgttt gatgtacaac agcttgaaat gccacaactt aacggctata cccaaccgct
    12421 ttatttgaat acgtggattg ataaattcgg ttatatgtat aacgatattc aactgattcg
    12481 caccgtgatg tacattgcaa tggttctcgt gattggcgtt gcctgtttta atattatttc
    12541 caccttgatt atggcagtga aagacaaaca aggcgatatc gcgattttac ggacactggg
    12601 ggcaaataac ggttttatcc gccgcatttt cttatggtat ggcttaattt caggtatgaa
    12661 aggggcgtta ttcgggctga ttttaggtgt aattttatcg ctaaatttaa ccgctattat
    12721 caaagcgatt gaagcattct ttgaggtgaa attgctttca gacggggttt attttgttga
    12781 tttcttacca agtgaattac attggcaaga tgtcgtctat gtattggcag ccacgattat
    12841 cctaagcctt tttgccagct tataccctgc caaccgagcc gctaaattag aaccggcaaa
    12901 agtgttaagt gggcattaat cattttccta ttctaataaa aaagcagcta cccctcgatt
    12961 atgtcagaaa ggtagctgct tttatttatg ttattactct tcgcctaata atttcaactc
    13021 acgagcctta atttggtttt ttagaatttc aactaagtct gaaacgaggt aagtacctaa
    13081 gtctgcacct ttacgggtac gcacagacac tttaccttcg gcgatttctt tatcaccgca
    13141 aaccaacata taaggcacac ggcgtaaggt gtgctcacgc actttgaagc cgactttttc
    13201 gttgcgtaag tctgctttca cacgaatgcc tgcatcagaa agctgtttca ccacttgttg
    13261 cacataatct gcttggctgt cggtaatgtt cattaccact gcttgggttg gggcaagcca
    13321 agttgggaag aaaccggcat attcttcggt gataatcccg atgaaacgtt cgatcgagcc
    13381 taaaatcgca cggtgaatca ttaccggtgt gcggcgacca ttatcttctg ctacataaga
    13441 ggctgctaaa cgacccggta gagcgaagtc aagttgcact gtgccacatt gccattcacg
    13501 tcctaagcaa tcacgcaatg caaactcaat tttcggcccg tagaatgcac cttcgccttc
    13561 ttgaatttca tattcaagtc cgttgttgcg taaggcgttg gctaagcctt tttctgctct
    13621 gtcccacatt gcatcatcac cgatacggtt ttccggacgg gttgaaagtt ttactgcaat
    13681 gttagtaaaa ccgaaggtgc tgtaaatgtc gtacaccatt ttgatacagc tgattacttc
    13741 tgactcgatt tgttcttcag tacagaaaat atgggcatca tcttgggtaa aaccacgcac
    13801 acgcattaag ccgtgtaatg aaccggacgg ctcgttacgg tgacacgaac caaattccgc
    13861 catacggatc ggtaaatcac ggtaagattt caagccttga ttaaagattt gaacgtgacc
    13921 cgggcagttc atcggcttaa ttgcatattc acggttttcc gattgggtag tgaacattaa
    13981 atctgcgtag ttttgccagt gtccggtttt ttcccaaagc acacgatcca tcataaacgg
    14041 accttttact tcttggtaat cgtactcttt caatttggta cgcacaaaag tttcaagttc
    14101 acggaaaatc gtccagccgt cattgtgcca gaacaccata cccggtgctt cttcttgcat
    14161 atggtataaa tctaacgctt taccgatacg gcggtggtca cgttttgccg cttcttccaa
    14221 acgttgtaaa tattcggcta attgtttttt atcagcccaa gctgtaccgt aaatacgttg
    14281 tagcatttta tttttgctgt cgccacgcca gtaagcaccc gctacttttt gtaatttgaa
    14341 gtggtggcag aaacgcatat ttggtacgtg tggaccacgg cacatatcaa tatattcttc
    14401 gtggtgatat aacgcaggcg ttgccgtacg ttcaatattt tcatctaaaa tcgccatttt
    14461 gtatggctca ccacgtttct caaaggtatc tcgagcttct tgccagctta ccgggcgttt
    14521 taccacgtca tagttggttt ttgccagctc taacatacgc ttttcaagag cgtctaaatc
    14581 ttcttgagtc aaagagcggt ctaaatcgat atcgtagtag aaaccgtttt cgatagtcgg
    14641 gccgatagcc attttaacgt ctgggaataa ttgcttgatg gagtgaccta acaagtgagc
    14701 ggttgagtgg cgaatgatct ctaaaccatc ttcgtctttg gcagtaatga tttcaagtgt
    14761 gctgtcttgg ctgatgatat cagacgcatc acggcgttca ccgtttacac gaccggcaat
    14821 ggtggctttc gctaaacctg ccccgataga ttgggcaact tccattacag aaactgggtt
    14881 gtcaaatgaa cgttgtgaac cgtccggtaa agtaataatt ggcataataa atccttacag
    14941 tggtaatcca tacgagagat tacttgtgtt gtttgaatga aaaaaccgcc tacaagcggt
    15001 cttttttgat taaaattttg caatttgctt attatttccc ttacaagtgg ggaataaaag
    15061 cactgaaatt ctagcataaa acaaagaaaa attaaacttt gctgttttca gcggcccctt
    15121 catcaagctc aatattttca ttttctgttt gtggatcttg ttcaagtatt tcaagctgag
    15181 cctggtactc ttgtaccgct tcttcatcaa atggaataag tgcgtcctca acaacttcaa
    15241 tttcttcaag tgggcttgcg gttgcaattg gggtagcata ttgcatctct gctcgttccg
    15301 ctttcggaat tgatttggtg cggaaagcac aaataaataa actaagtgag aacagtccaa
    15361 aaagtgcata aaagccatat tcagcggaaa ggctcatcgc aaaaccgata agcggtggag
    15421 caactaacgc ccccacacca tagcaaaaca gtaaacccct gcttacttca accgtgttca
    15481 tattattcgg caattcatcg ttggcacggg caatgctcaa agcatataag ctaaacagac
    15541 tacagccaaa gaaaaaggcg ataatatact gtccccaggc ggtcaaattt tcttggaaaa
    15601 ttgcaaataa tccggcaagt gcggttaaag tggcaatcac tgaacaagct aaaatggcgt
    15661 tacgtctgcc aatacgattc gataatatcg ccatcggcat ttggctgata aaaccaccga
    15721 tcatcgagat catcaaataa agcgagattt cacttaatga aaagctaaac ttcagcagaa
    15781 agactgacgc cattgtaaaa aagccgttta ccaacattcc gctaataaag ctggttgcta
    15841 aggctaacgg tgaaatcgca aatagtttag gaatactaat gcgctctcga gccggaatat
    15901 caggctgaac cattttagtt aaagcaattg ggatcatcga catcacaacc aaaatggtag
    15961 ataagataaa aatgtttcgg ctactaagtt ctaaacttaa taaacttacg cctaaaataa
    16021 aggcgagata gaagataatg ttataagtgg caagcacttt gcctctggta tcgacattgg
    16081 ttttttcgct taaccaactt tcgactaaaa gcagcaagct ataatgacag aatcccaaaa
    16141 taattcgtaa cacgccccag atccacaaag tatcaatcat aatatgaccg agagcggcga
    16201 tagcaaaaac agcacagaaa acgctaaatg aacggatatg cccaacactt gatacaatgc
    16261 ggtgagcaaa aatagtgctt aatgccgccc caataaagaa agcagcgtta agtaaaccga
    16321 tgatgatgtc cgttatcccc atttctgcca gtttgatccc tgctgaattt agaaataaac
    16381 cgtagccgaa aaacagaaaa aagatacctg aataaaggga gtaaaattta gtgctattca
    16441 tcaatgctcc ttcaaacatt ctgccagtat taaatcattg taccgattta atacctttta
    16501 ggcaaaaaag ttattattag cgttgagaaa ataacttttg gctattttga ttaaaaaatc
    16561 aaatgaaggt gattttgaaa tgtagtaaaa aatagagcaa tcagaattga aaaagggttt
    16621 gccgtaacaa agcctttaga aagattagag tttcgagaag aatttgtagc ttgggctaac
    16681 caaaacactt gctaggagtg ctttaagcac accacctaaa ataaatggat ataagcccgc
    16741 ttgtaatact gtgccttctg gtacaaagaa agttgagcca agccgaaagc aaaggtgatc
    16801 acacttgcaa ttaaggcgac aaataaactt ttccaaaaag agtttaatac gcctttatcg
    16861 ctataatagc ctaagaaata ggcggttgga atatagccta ataagtatcc gcctgttgga
    16921 ccaaagaaat gatggaaacc tgaagcaccg ccggcaaaga caggtaaacc aatcgcccct
    16981 tcaaaaagat aaagtaatac tgcagcagtt gcggctttac gacccattgc aaaaccaacc
    17041 acagttaaag caagtgtttg ccctgtaatg ggtacagggt acatcggcac agaaatttgt
    17101 gcaaaaatag caagcagtgt tgctccaata aaagttttca accaaaatgt agttatacta
    17161 ttggcttcta gccaacgttt agtttgaatt aattgcgtag tgaacataat cctctccttt
    17221 aatatggtta aaatgttctc aaccgattat ataagaagat gtcttttagg gtaagagtga
    17281 atttaaaatg gtaaaaccag tttatattta ttgtgatgaa ggtagcagtg aaattggtgt
    17341 tcaatcactg ttattggcag taaaacagca tttacagcgt ccggcttatc tcatcaatgc
    17401 caaacaaatt attcaaagca agttttccga tggctgttta ttaatcatgc ccggtggagc
    17461 cgatttacct tactgcaaaa aattaaacgg agaaggcaat cgtagaatcc gccaatttat
    17521 tgaacaaggt ggagcttatc tcggtatctg tgccggagct tattatggct gtgctgaatt
    17581 gaattttgta gggcgtgact atagcgtaaa tggtaatcga gaactagctt tatttaatgg
    17641 cattgcgggc attgcgaaag gcagtctgcc gcagtttacc aacgggcaac tttacgataa
    17701 aaacattggt agcaaagcga tagttgaagt aacacttgca gagggtggaa aaatgccgtt
    17761 ttattatcac ggtggttgct gttttgaacc aaattcaaca acagtgattt acaacccgtt
    17821 ggcttattat cctgatggct cagtagcaat gatagaggga gagctagggc gaggttacta
    17881 tctgttgtcg ggtgtacatt ttgaattaca agcggtcgtt tattggcaaa aaattgtaaa
    17941 ttgctgtgaa aatcttgaca taaaaacaca agaagaagca atttgctcta attttaatgt
    18001 aaattatggc tcgcaaattt ggcaacatct aaagcaacga ctaaatcaag cgtaaaaaaa
    18061 taacctttcg ctattctgcc aaatggtttc tgcaatcaga tttggtgatt ctgttcttaa
    18121 ttcacataga ctattgaaag tcactaaaag ccgttccggt cggttcggct ggccttgaaa
    18181 gccaaacacc ggcatatcgg gcgagtcggt ttccagtagt aaactttcaa gaggtagttt
    18241 gcggatagcc tcacgggttt tgttagcacg ttcataggta atagttcccc ccacaccaat
    18301 tttataaccg agatcgacaa aacgttttgc ctgttcatag ctgccggcaa agccgtgaac
    18361 cacaccggtt ttggttagtt gggctttttt gaggaacaca aaaagttgat cgtggctttt
    18421 acgtgaatga agattaacgg gtaaattaaa ttttttcgca agggaaagtt gtgcctctaa
    18481 aaattcacat tgctttttcc ataattcagg ggtagaaact tcacttaccg ctttttccaa
    18541 accgatttcg gcaatagcgg tacactgtgg atcttgttgg cttagcaaat tttccaataa
    18601 gattaaatct tgttgttggt gctgttgaat atacagtgga tgcaacccta atccatacac
    18661 caaattttcc ggtttttgtt tggcacaagc ggtcactttt gcaaaatttt ttgcaaatac
    18721 ggcaacgata agaatgcatt caacctgatg ctgtttggca ttctcaacca attccggaat
    18781 gcttaaattc aggctttctg cgagatagtc gaggtgagta tgagtgtcaa aaaatcgcat
    18841 aataaaacca ataaaaacgg ggactagatt gtccccattt ttagcatttt acgcaagcaa
    18901 ttagtttgtt acataagcat cttcatcacg agcctcagat ggcttgagga aagcaaagaa
    18961 gcaaataaca caaactaccg taatcgcaaa accgatataa actgataatt cataatctaa
    19021 accgaagccg attttgttgt tcgcaaggta agttgcacac acggtagtga taaaccatgc
    19081 aggaatcgag catacccagt ggaatttttt atagcggtat aagtaacctg cggcagtcca
    19141 taacataacc attgcagtca tttggtttgc ccaagtgaag taacgccata atacgctgaa
    19201 atcaactttt gagacaatga aaccaagtac gaataatggt acggcaatat acaaacgttt
    19261 tactaaggta cgttgttcta aaccaaagat ttccgctagt tgtaaacgag cggctcggaa
    19321 tgcagtgtca cctgaggtga ttggtaatac tacaacgcct aatacggcaa aaataccacc
    19381 gataaaccct aagaagtgta acgagctgtc atataccact ttagacggtg aaccggcaga
    19441 gatcgcatct tgtaaggctt gtgggttttc atagaatgaa agacctactg cacaccatac
    19501 taatgcaatt accccttcag taatcattgc accgtagaaa ataaaacgac cttctttctc
    19561 attttgcgca cagcgagcca ttaatggggt ttgggttgcg tggaaaccgg ataatgctcc
    19621 gcaagaaata gttaagaata gtaatggcca aattggaaca tcacctttca cttggaagtt
    19681 ttgggtgaat ttttcccacg ttaagccttg accgtctgca ttcactgtac ggaagaagtc
    19741 gataggatca gtcgcactta agtgagcgct gactagacca tataccatac ctacagacat
    19801 aaagagtaat aatgctccga agaaaggata aattcgaccg atgattttat caacaggtag
    19861 taatgtagca agaatatagt aagcaaaaat gattaacgtc cacacagaaa tcacggttgc
    19921 tttatccata ccccaaacgg ttacgccacc tgatgctaca gattctttaa ttgcttcaac
    19981 atcacctaat tccattgcac tttgggttgc acctgcaata tccatggtga tggtagagag
    20041 taactgagca gggcttgcaa cgaaaactac gcctactaat aaaagcaaga tgagcgccat
    20101 tacgttgatg aataacttga ctggacgacc caagaatttc cctgccagat ttggcatcgt
    20161 tgcgccaccg ttgcggatac ttaacatacc acagaagtag tcgtgtactg caccggcaaa
    20221 aatacaacca atcacaatcc aaagcattgc aactggtccg tataaggcac caagtattgg
    20281 tccaaaaata gggccggtac cggcaatgtt taataattga atcagccaaa tcttggtttt
    20341 agacattggc atatagtcaa caccgtcgtt cattgcataa gccggcgttg ctcttttcgg
    20401 gttaatcacg aagatttttt caatgatttt tccgtaagta aagtagccaa gaacaagcac
    20461 ggctacacag aaaaagaacc acaacatagt agaaatccta taaattgaga taaatgatca
    20521 tttttagcag aactgataaa ggcgtgccat tgtaagtgag gagtgaggat aaagtaaaat
    20581 gaattgttag atttgtgtga ataatttact gaaaatgtga ggttcatcaa aaaatccctt
    20641 tgttctgcgt caatcaaagg gactttttaa agttaaatat cgaatttttg aattttcgct
    20701 tcaatgcctt gggcatctaa tccaagatcg gcataagctt cagcttgagt agattgggga
    20761 acgaaaaagt caggaatacc aagcattaca agcggtttga ttttgccgat attttgcaaa
    20821 tattcattca caaaactacc tgcgccacct tgaatagcat tttcttcaag cgtaactaac
    20881 aactcgtgtg aatccgccac tttttgaagt aatgcttcat ctaatggttt aatgaaacgc
    20941 atatctacca acgtgtagtt atgtttttca gccacaatct ttgcttcatc aagcaaagca
    21001 ccaaaattta aaatcgcaac ttttttacct tcacgaataa tgtttccttt acctacttca
    21061 agtctttgca ttggctcaag ttttacccct ttagcattgc ctcgagggta acgaatagca
    21121 cttggcgtat tcatcgtgta agcggtataa agcatttttc gcatttcatt ttcatcgctc
    21181 ggtgccataa tggtcatatt gggaatacaa cgcataaaac ttaaatcaaa tgcaccttgg
    21241 tgagtttgcc cgtcagcgcc gacaattccg gctcggtcaa tcgcaaaaat caccggcagg
    21301 ttttgaatag caacatcgtg aattaattgg tcataagctc gctgcaagaa gctggaataa
    21361 attgcgacta ccggtttgta acctgcgatt gctaagcctg ccccgaaagt cactgcgtgt
    21421 tgttcggcaa tcgccacatc aaaatattgt tcagggaaac gtttggaaaa ttctaccatt
    21481 cccgagcctt cacgcattgc cggcgtaatg ccgataattt tcggatcacg ctctgccatt
    21541 tcacatagcc aatcgccaaa aatattggaa taagtaggtg ctgcttttga ttttggtaac
    21601 tcggctgcgg tagggtcaaa tttcggaacg ccatggtagc caatcggatc attttccgca
    21661 ggtgtgtagc ctttgccttt tttcgtgcga atatgcagaa attgagggcc tttcagctca
    21721 cgcatattgc tcaaggtttt gactaactcg tcaatattat gtccatcaat cgggccgata
    21781 taattaaagc ctaactcttc aaacaaagtg ctttcagggg agaaaatcac ccctttcata
    21841 tgctcttcgc tttttttcat aaagtttttg atggtaggca ctttatctaa aattttctta
    21901 ctgccgtcac gcactgaagt atagagcgaa ccagaaaaaa tgcgggcaag gtggttgttt
    21961 aatgccccga cattttcaga gattgacatt tcattatcat ttaagataac caacatatcg
    22021 gtatgcaatg cacctgcgtg gtttaatgct tcaaatgcca ttccggcagt gattgcacca
    22081 tcaccgatta cacagacggt tttacggccg gcattttctt tttctgctgc caccgcaatg
    22141 ccaagtccgg cactgatcga agtagaagaa tgcccaacgc ttaacacatc gtaagggctt
    22201 tcttcacgcc aagggaaggg gtgaatacca tctttttggc ggatagtgtg catttgatct
    22261 cggcgcccag taaggatttt atgtgggtaa gcttgatgac caacgtccca aattaattgg
    22321 tcgaaaggcg tttgataaac ataatgcaaa gctaccgtaa gctctactac ccctaagcca
    22381 gaggctaaat gtccgctagt ttgactgact gattccaata aataggctcg cagttcatca
    22441 actacggttt gaagctggtc tttagaaagt aaacgtaaat cttccggtga gttgatttcg
    22501 cttaacagag gatacttctt ttccataatg ttttattacc cacaaccatc aaataataga
    22561 tgatattaaa ttaagctttg agttcttcgg tcaaagtagg ctgaatgcta tttaaaattt
    22621 ctttaaccac acggccgtta ccaacaacca gattacctgt tttcaagtaa tctgttccgc
    22681 caacaaagtt agtcacaatt gcaccggctt cacgggcaat aagttcaccg gcggcgaaat
    22741 cccacggttt taagccgatt tcaaagaaac catcaacacg acccgctgca acataggcaa
    22801 gatctaaagc ggcagaaccg gtacggcgga aatcagctac tccgttattc attaagcctt
    22861 caagcatatt taaatgtgca tcacgatgtc tgatgacttt gaaaggaaaa ccggttgcaa
    22921 ttacagtgcc ttttaaatca cggcgttctg catcaatgcg taaacggaac tcgtttaatt
    22981 tagccccttc gccacgcacc gcagtgaaaa gctcgttacg gataggatcg taaactaccc
    23041 ctactgttgt acgtccgttt tcacgcacgg caatcgacac agcaaaatga ggtaagcgtt
    23101 ttacgaaatt ggttgtacca tctaatggat cgatcaccca ttgtacattt tcattgctac
    23161 cgtttaacac gccggactct tcggtcacga tcgagtgttc agggtaagat ttacggatca
    23221 catcaataat tgccgcttcc gctgctttat cgatttcagt cacataatca ttcgtatttt
    23281 tttgtaaaac ttgtgtatct tgaggcgatt gttcatagcc tttggcaata atattgcctg
    23341 cttttcgtgc cgcacgaata gcaatgttta acattgggtt catatttcca ccagatttta
    23401 aagaacataa taaagcacga attataaaga atgtagcgaa ataaagaaac aacaagcggt
    23461 ctaatctgtg aaaaattttg caaaaaaatg gctttttcta accgcttgga gtttggattc
    23521 gttataatac ctcccattca agccacccat ttaggtacta aaaatgcaat ttaaagaaaa
    23581 taaccaacct cgttttaaac acagagaaga acgccgttca gatgagtgtt ccccccgttt
    23641 ttcagataaa ggcaaaatgc gaggaaatga aggtaaaagt gacagaccgc ataagctaca
    23701 ttttaagcgt gacgaggctt attcacgctc tgagcagatt tatccagcag aaggcaaaaa
    23761 agccagtggt gaaggtagtg tgcaaatttg ggtaaaaggt gggaatagct cagttaaaga
    23821 gaaaaaaaac ggcccgcttt caccgagagc gcctgagaaa attaagaaaa atcgcttgga
    23881 agagatgaaa gtctatggcg aaaatgcctg tatcagtttg ttccaacaac gtcctgaatc
    23941 tattgtacgt ttatgggcaa cagtggaggg ggcaaaaaaa ttaggcgatt taatgagcta
    24001 tttggctcag cacaaaaaag cctatcacgt tattgaccgt gaagagatgg agcgagtcac
    24061 cggtactgaa catcacggcg atatgtgctt attggtgaaa aaaacggctt catttgtgct
    24121 agagggctat ttacaagtgc cgaaaaaaca agattgcttg gtactgttag atggtgtaaa
    24181 caatgctcag aatgtggggg gaatcatcag aacgtgtgct ttttatggtg tgaaaggcat
    24241 tattactgaa aatgtggata gcttaaactc cagtgctgca gctcgtgtcg cagagggtgg
    24301 tttggaattt gtgcgagcgt tagaaactaa aaacaagcat attgctttgg tacaactccg
    24361 ccaagccggt tatcaaattg tccacctcac acgccataag caagcccctt ctttaaccaa
    24421 aattaagctg gcggaaaagg tggtttttgt attaagtgag attcctgcaa gtgatgtggc
    24481 ttacgcagaa gacacaacaa tacagctttc ctttaataat ccgttaaata gtggtttaaa
    24541 tgtggcggta aacgcaggta ttctacttaa tcaatggtat caaacccaca ttctttaagg
    24601 aattgaaatg aatcagcaaa aaccgtttga agtgattgcg attgttggca cacctcgcca
    24661 cgacagtgca tttgaaacac atcttgccgt ttataattgg ttaaaagatc gtgattatca
    24721 aattttagtg gaaacagagg ttgccactca gttacagctt aaggaaagct acagtttaga
    24781 tcagattggg cagcgcgcag agttggtgat tgtgataggt ggcgatggta atatgcttgg
    24841 catcgcccga gctttggcta aataccgagt gccactgata ggcattaacc gtggcaatct
    24901 gggtttttta accgatatcg cacctcaaac ggcttttgaa cagctttata gttgcttagt
    24961 caaaaatgag tatgtgattg aagagcgatt cctgttagag gctttcattg agcgcaacgg
    25021 taaaattatt gccgcaaata atgccttgaa tgaaattgtg gttcacccaa cccaagtggc
    25081 tcgaattatt gaatttgaag tttatattga cggaaaattc gctttttcac aacgtgcaga
    25141 tggcttgatt gtggcaacgc ctacaggctc aaccgcctat tcactttctg ctggtggccc
    25201 gattttaacc ccgaatatga atgcaatggc attagtgccg atgcaccctc acactctttc
    25261 ttctcgtcct ttggtggttg atggcgatag tcaaatttcg cttcgttttg ccaaatataa
    25321 tacccctgat ttagaagtga tttgtgacgg gcaggtgaat atgcacttta cgcctgaaga
    25381 tagagtcttg gtgcgtaaaa gcccggataa attacagctg ctccatttaa aagattatag
    25441 ctactttact gtattaggtt ctaaattagg ctgggcaagt aagttgtttt aaacacaagc
    25501 ggtgtttttt ctgattaaat ttgcaaatgc aatatgaatt gatttcatat acatttcaaa
    25561 aatatatcat ttttattcaa taaaaatttg tggttcaata agctatctga aaaataaagg
    25621 ggacaataaa atgacaacaa attttaaatg ccaagtgcat actattgaat ttaatgaaga
    25681 ttaccaaccg gagcaaagca cccgtttaac caccaacttt gccaaccttg cccgtggcga
    25741 aaaccgcaaa cagaacctgc aaaatgcgat tgcgatgatc aataaccgtt ttaacgattt
    25801 agcgaactgg gataacccga atgcggatcg ttattcggtg aaattggata ttgtctcact
    25861 ggatattgat gtggaaggca aaggcgagtt tttccctgcg attgagtggc tgaaaaccta
    25921 tgttatcgac tacaaaaccg gcaaaaccat tgatggcatt atcggtaata acttctcttc
    25981 ttttgtgcgt gattacgatt tcagcgtatt gttgttagat cacaataaag acaaaccgca
    26041 atttagcgtg ccggaaggct atggcgagtt acacggcaag ctgtttaagc atttattggc
    26101 atcgcctgaa tataccgcaa aatataaaaa accaccggtg atctgtttaa gcgtggcaac
    26161 cacccgtacc tacacaagaa ccactaacgt tcacccaatt ttaggtgtgg aatatctgca
    26221 agatagttat tcattaactg atgcctattt tgcgaaaatg ggcttaaaag tacgttactt
    26281 tatgccgcca aaatccgttg caccctatgc gttctacttt gatgagaatg cagatttact
    26341 caatgattac actaacttag agctaatcag cacgattgcc acaatggaag cgttccaaaa
    26401 aatttaccgt ccggaaattt acaatgccaa ttcggtcgca gggcaggtct ataaaggcag
    26461 cctgacttac caagattatt ccgtcaccaa agtggaatat gaccgtgaag aacgcaccca
    26521 gttggcgatc aaacagggta aatacaccga agagcatttt attaaacgtt accaagcgat
    26581 tttagaggct tgggcgacgg aggaataaac gtagggtggg cgtaagccca ccataaaatg
    26641 agatataacc aatggtgggc ttacgcccac cctacttttt ataggaaaat tcaaaatgag
    26701 caacattctt ctcccaacct ctaccgccgg cagcctacca aaaccttcgt ggcttgccga
    26761 gccggaaaaa ctgtggtcac cttggaaatt tgaaggcgag gaactacttc aagccaaacg
    26821 tgatgccctc cttgtttccc ttgccgaaca aattgcggca gggattgata ttgttagtga
    26881 tggcgaacag acccgccagc actttgtgac cacctttatc gagcatttgg aaggggtgga
    26941 ttttagcaag cgtgaaatcg tgcgcattcg taaccgttac gatgccagcg tgccgagcgt
    27001 ggtgggggca gtttcccgcc cgaaatcggt gtttgtggac gatgccaaat tcttgcgtgc
    27061 gcataccgac aaaccaatca aatgggcatt acccggcccg atgacaatga tcgacactct
    27121 ctatgacggt cattacaaaa gccgtgaaaa attggcgtgg gaatttgcca aaattctcaa
    27181 cgaagaagcc aaagagttgg aagcggcagg ggtggatatt attcaatttg acgaaccggc
    27241 atttaacgtc ttcttcgatg agctgaacga ctggggcgtg gcggcgttag agcgtgcggc
    27301 ggaaggctta aaatgcgaaa cggcggtgca tatctgctac ggctacggca ttaaagccaa
    27361 taccgactgg aaacagaccc tcggcaacga atggcgtcaa tacgaagaga gtttcccgaa
    27421 actgcaacaa tcaaaaatcg acattatctc gttagagtgc caaaactccc gtgtgccaat
    27481 ggacttaatc gaattagtgc gtggcaaaaa agtaatgctg ggagcgattg atgtggctac
    27541 caacattatc gaaacaccgg aacaagtggc agatacgtta cgcaaagcat tacaatttgt
    27601 cgatgcggat aaactttatc caagcaccaa ctgcggaatg gcaccacttt cacgccaagt
    27661 cgcacaaggt aaattacagg cactcagcca aggggcagcg atattgcgtg cggaattcgc
    27721 aaaataattt tgcgatctgc atcgaaaata tcaactttgc tttttgctcg tttccagatt
    27781 ttcttccaaa ataacaggaa atgacggagg cactatgcta aatctattta aaaagccaat
    27841 taagaaaagc tcctttgagg cttgggcaaa actaatggac gatgtggcaa aagttgcaat
    27901 tttagcaatt cctgttattc tctatggaga atattcactg ctaatgaaat ttacacactc
    27961 aattacctta tttgcagtag tttatttttt gttagcaacg gcaagattgt ttcgagaaac
    28021 agcagaggag atgtaaatgg acggattgac aatcggatta tttcttttgc ttgcattttc
    28081 atttgctgca taccaatggg caaagcattt aaaagatcat aatcattaat tcaatgaata
    28141 atactaagcg gtcatatttg gtaaattttt tacaaaatgt gaccgcttgt ttttctttca
    28201 ctcgaagtta atttttatat aaataatact atctatgaaa ctaaaaaatg cgtaaaataa
    28261 cgtgaaatca acttacgcaa acgtttactt aagcttatta ggagtccaaa aatgttaaaa
    28321 cgtgatatga atatcgctga ttacgatgca gtattatggc aagccattca agatgaagat
    28381 cgccgtcaag aagaacatat tgaattaatt gcatctgaaa actatgcaag cccacgtgta
    28441 atgcaggctc aaggctcaca attaaccaat aaatatgcag aaggttatcc gggtaaacgt
    28501 tactatgggg gttgtgagta tgtggatatt gtagaacaac ttgcgattga ccgtgcgaaa
    28561 gcattgttcg gggcagatta tgccaacgtt caaccgcatt caggctcaca agcgaatgca
    28621 gccgtatata tggcgttgct taatccgggt gacactattt taggtatgag tttagcgcac
    28681 ggcgggcatt taacccacgg tgcttcagtt agcttctctg gtaaaattta taaagcagaa
    28741 caatacggca ttactgatga aggcttaatt gattatgaag cattacgcaa acaagcccaa
    28801 gaagtgaaac cgaaaatgat tgttgctggt ttctctgctt attctcaagt tatggattgg
    28861 gcaaaaatgc gtgagattgc cgatgaagtt ggggcatatt tatttgtgga tatggctcac
    28921 gttgcaggct taattgcggc gggagtttat ccaaatccgc ttcctcacgc tcacgtcgtg
    28981 acgacaacaa cccataaaac cttagccggc cctcgaggtg gtttgatttt atctgcatct
    29041 ggcgatgaag aaatgtataa gaaactgcaa tctgccgtat tccctgctgg tcaaggtgga
    29101 ccattgatgc acgttattgc ggcaaaagcg gtttgtttta aagaagcttt agagccggaa
    29161 tttaaggcat atcagcaaca agttgtaaaa aatgcaaaag caatggttga ggtatttaag
    29221 caacgtggtt ttgatgtggt ttcaaacggt acggaaaacc atttattcct tgttagcttt
    29281 gtaaaacaag gtttaaccgg caaagcggca gatgctgcat taggagctgc gaacattacc
    29341 gtgaataaaa actcggtgcc gaacgaccct caaaaaccat tcgtgacttc aggtattcgt
    29401 gtgggtacgc cttctgtgac ccgccgtggt tttaaagaag cagaggtgac agcattagcc
    29461 ggctggatgt gcgatgtgct agatagcatt ggtaaggata atcacgaaca ggtgattgca
    29521 gagactaaag cgaaagtttt agacatttgt aaacgtttac cagtgtacgc ataatctcaa
    29581 ctttacactt tttcaagcgg taatattttt tagaaatatt accgcttttt gttttatcaa
    29641 tcattttctt tatctgtttt ctgaaaatgg attgattttt ctttttttac ataacataat
    29701 gataataatt ctcattaatt tattttttgt tatgttacct cgtatttcct cttttttagc
    29761 tagccttttt atggcttttt ttcttttagg ctgctcggtt tcaacgcaat cccttaatga
    29821 acaaaacgtg gactctatcg gccaaattat tgatctgaat accaatcaaa cgatttcatt
    29881 tactgaacta ctttcccgtc tatcttctca agattacgtt ttagttggtg aggagcatac
    29941 tcagttaatt caccaccaaa ttgaattgta tttatttaat caacttacga agaaagccaa
    30001 gttacattta attgcgttgg agatgttaaa tgtggatcag cagcctgcga ttgatcgcat
    30061 tcagtttaac cgaccggtta atttatcagc cacggattta agagaggcaa ttcaatggca
    30121 aaaatgggat tggaagatgt atcaagattt agtggtagag agcttaggtt caaatagtcg
    30181 tgttatagcc actaacttaa cagataaaga ggttgaaatt ttattaaatg gggcggagcc
    30241 attaaaaggt tctgtatcca cttcctctga agttaagcaa aagatcgcac aactgttgct
    30301 gtcaatgaat cacggaatgc cttacccgat ggaaaatatg gtggcagtac aacaattccg
    30361 tgatcgcaga atggcagaaa aattagtaaa aaatgcgtta tcgactagct tgctgattgc
    30421 aggtaaccac cacgttcgca aagatcttgg cgttccgttg catattgcgg aatatgaccg
    30481 aacaaaaaaa gtagcggtgc taatgcttaa aacagagaga gaagaaatta cttcttcaca
    30541 ggcagattat ctttgggtca ctcaataagg agaattatga aatttaatat aatcacacgc
    30601 attttgtttc cggttttgtg tatgggagga tcgcaaactg cattggcaga ggaaaaggcg
    30661 aaaaatattg cggagcttga tactgtgacg gtttatggca ctgtggaaaa ccctaatttt
    30721 gagaatttca gtaaagccgg ctcggtgagt tctcgagcaa tgaatgtgaa tgcaatgcaa
    30781 agtttagata gtattgtccg ttctgtcgca ggtggctata cccaaattga cccggggcaa
    30841 gggggcgtga gtgtgaatat tcgtaatatg agtggtcttg gtcgggtaaa taccttagtt
    30901 gatggcgtgc cacaaacaca gcttggtaca tcagcaaacg gaggtggtaa gttccacact
    30961 gatggtaacg ggccaatgag ccaattcagc actttaattg atcaaaattt tttaacccga
    31021 gttgatattg cgaaaggcta caacaatggt gcagccggat tgaatgcttt agccggtagt
    31081 gctaatttta aaacgattga tgtcgaagat attctactag aacaacataa agtcggcgta
    31141 ttaagccgtt ttaattatgg caataatgat ctgggaaaaa acggaatgct tgcactagcc
    31201 ggaaaagttg atgtgctgga aagtggttct cttggcagtt tattggcttt tagcaaaagt
    31261 agtatcagtc aaaattatcg ccgaggtaat ggcacaatgg caacggattc tgcttatatg
    31321 caagggttaa aaaatcaccc tgaatcttat atgggaaaaa ttgcctttaa gccaaatcaa
    31381 gattcagagt ttaaattgtc cggacgtcaa tataaaaata gcttagtggg gcgtaatatt
    31441 gatagtgtga cctattcctt agaatccagt taccagcctc aatctgattg gattgatctg
    31501 aaattcctaa cagcagtaac agaaactaag caagatttcc acgatgatgc aaaactatgg
    31561 acattagaag atgcctcaac caaaaacaaa atgtggatgt ttgatttgaa taacaccagc
    31621 cgttttgagt cagggaaata aatattcccg tgatttaccg gaaagtaaga ataatgatgg
    31681 agcgattagc aatgccgctt ttgggccggc aggtaagcaa aatattgatt ccctttacct
    31741 agacaccgaa tggaaatacg gcattgtcac tttaaattca agtcttcact attcacgcta
    31801 tcagttaaaa ggatataagc cagaatgcga tagcgatgat atggattatt gtttcccttc
    31861 ttttgggttt gatgttaata aaaaacatta taagttgaat ccttctgcca ctttagcatt
    31921 tcaggttgct gattggtttc aaccttttgt cagttacaaa caaacctccc gttcgcctaa
    31981 tgtgcaagaa atgtttaata ccagtcgaaa tggcttgagt gtgaacccgt ttttgaaacc
    32041 agaaagtgcg aaaacctatg agattgggtt taatattcat caagataatg tattactcaa
    32101 taatgatatc ttggggctaa aagcggtata ttttaactca cgcattaaag actatttgta
    32161 taacctctcg ttgtatggtt gtgggccaac agtatgtcga aattcaagtg atgctaccaa
    32221 tgctatacag ttccaaattt atgtgaatca ccctgaaaag gttaaaaatc gaggttggga
    32281 agttgagctg gcttacgatt ccgatgcttt ttttgtcaat ggtagttata cccgaactga
    32341 aagcacagca cccacttcaa aaggtgcgac tattctttat ggatttggca ctgaagctta
    32401 tacccagcta ccaaaagatt atgcgacttt ggagctaggg gctaaattgc tggataaaaa
    32461 attaacactg tctagcattt ttaaatatac aggcaaagcg aaacgtgtcg gtattgattt
    32521 agacgaagat ggcaacatta ttcacgagca gttaccgaat attccggtga ttatcgattt
    32581 atatgcaaat tacaaagtga ataaacattt aaccttaaaa gccggggtac aaaatgtgat
    32641 gaaccgaaat tatcttgatg ccttaaatgc gtataactct tccaccgcag aagagtatga
    32701 ctatgataca gacacctatc gtttcacaaa tagtgccaga ggtcgaactt acacactagg
    32761 ggcagaggtg cgtttctagc ttgaaagtgg cttattttaa ccaatacgcc ctttttcttt
    32821 cctgtactga tgttgagatt caaggcataa tatccagaac gatgaatatt cagccggtgt
    32881 gtaatatgaa aaaatttgtg gttttaattt cagggaatgg ttctaaccta caagcaatga
    32941 ttgatgcgca aaaatctgcg gatacaagcg gtcaaatttg cggtgtaatt tgcaataagg
    33001 cagatgccta tgggctgatt cgagctaaac aggcgggaat cccgacattt gtattttctc
    33061 gtaaagatta ccaaagcaat gtggaaatgg atcttgcgat tgccgaacaa attgagcagt
    33121 taggagctga gttaattgta ttagccggct atatgaaaat tttaacgcct gaatttacgc
    33181 aacattttgc tggcaaaata ctcaatattc acccttcctt attacctaaa tacccagggc
    33241 taaacactta tcaaagagcg atagaggctg gggaaagtga acacggcacg actattcatt
    33301 tcgtgaatga agaagtggat gctggtgcgg tggtattaca agctaaagtg ccgatttatc
    33361 ctgaagatga gattgaagat gtaatggctc gagtggttga gcaagaacat cgatattatc
    33421 cgttggtgat tgagtggttc tgttcgggta gattagtttc tcagcacggc aaagcgtatt
    33481 tagacggtaa actactgcct gcttgtggat atgcagaaga ttaaacgata gggtgcatcg
    33541 caccctaact ttcagctaat gcagggcaaa gataatattg cccgctgtga gggcaatagc
    33601 gacagcccat tttcatttca tacagctcat ctaataaaca actttctgca atggctttag
    33661 cggacatttc agcaaatttt tctccatttt ttagcattaa gatatgatcg gcttgattta
    33721 ttgccaatgc aggatcgtgc aaaatcgcaa tgatagttaa gccttgttgt ttcagctttt
    33781 gtagtaagga aaaaagctga tattggtgat gaatatccaa atggttggtg ggttcatcaa
    33841 gcaaaagcca gcttccgctt aaatcggcat tcggttcagg aacgagttga gccagcactc
    33901 gagcaatatg cgctcgatgt tgttctccac cggaaagttg tatgctactt ctttctttta
    33961 atgcggttaa agacattgtt tccatagccc aagcggttat tttttcatta aattttgcaa
    34021 attcactttg gcgatatggc tctcttccga gttgtaccaa ttcttctact tttaagggaa
    34081 aggcaatttg gctctgttgg ctgagcattg cccgtttttg agccagagca tatccgttta
    34141 agtctgttaa gcgttgatgt tgataaaata cggcactgtt agtttgtgca ttattaccgc
    34201 ttaaggcttt gagcaaggtt gttttgccgg ctccgtttgg accgatgatg gcggtaaatt
    34261 ttccttgttc aaaggcaatt tcttgaatct ccagcaactt tttttgttga tgaacgatgc
    34321 ttaaattttg gcaagttaat aaattgctca ttagatatgt ttcccacttt tccataccat
    34381 tactgcaagg aatggcgcac caaataaggc ggtaaaaata ccaatcggga tttctgccgg
    34441 ggcggctagg gttctggcaa gagtatctgc aatcattaat aagattgcac cgcaaaccaa
    34501 actattgagt aataaatggc ggtggttact gcctcccagt aaacgcatta aatgtggaat
    34561 aaccagcccg acaaagccga tagccccaac acaggcaacg cttgtgccgg ttaataaggc
    34621 aacgtaaaac acaagacgac gtttgacttt tgcaatgtca aaaccaatgt attgtgcatc
    34681 ttcttcacct aatgttaagg cgtttaacgc acggtgttcc cgccaaataa gtgggctaca
    34741 aatgaagaca accgcagcca tcattgccac catttgccaa ttcatacctg ctaaagatcc
    34801 catactccaa aaggttaaac tacgaagttg gttatcatcg gcaacataaa tcagtatgcc
    34861 ggttaaagcg ccactaaatg cagctagagc aataccaatt aatagcataa tggcgatatg
    34921 tagttgcccg ttacgttgag aaagtaaata gagcataaaa gtcaccagcc agccccctaa
    34981 aaaggcggca ataggtagag cataatagct gattgattcc ggcagtagtg gctgaatcac
    35041 cacaatatat aatgccgcag ccaatgcagc acctgaagag acaccaataa tccccggatc
    35101 tactaacgga ttgcgaaaca gcccttgcat tgttgctccg ctaatagcga gggctgcccc
    35161 gatgaataat gcccctagta atcgaggcag gcggatttgc cataaaatat aggctgcact
    35221 ttcgcattca gcgttgttgc agtgccaagc ggataggggt aatgacatcg cccctaaatg
    35281 ggtacaaatc actaagctga taattaaagt gaaaattaag gttaaatgca gtttcaaagc
    35341 gtatccgtta ttttaaacaa ggttgaatag tggtgagaat ttggtgtgca tattgagcag
    35401 tgtaaggccc aaagcccaag gcatctaaaa tattgattgc aaaaacacag ccttttttgc
    35461 ccgcttgtgt atttgtgagt tgggcgtatt gtgggctgga ttctgctaat ttactgatcg
    35521 ttgcagattc ttttgcctga ttacgggagg ctaataaaat aacatcggca ttgcttgcag
    35581 ctaaagcctc attggcgagt ggcttattgc cctcagcatt gaaactattg tttagattta
    35641 acaaggttaa gaaatgatca cccacactct ttttccctag cccaaagata ccttgcgatg
    35701 cagtgtcaat cagcactaag acttctttgt cagtagaatg ttggctacga atgtcggcta
    35761 attttttttc atctgccaga atggtttctg ttaattgttt tgcctctgat tctgcaccaa
    35821 tcgccttggc aatttgctga atatcctcac gaatccctgc taagctcggt ttctctccta
    35881 agtgaagtaa tggaataccg ctggctttaa tttgctcaat aactgcgtta gggccggcat
    35941 caggggctaa aataatcaaa tctggggtta aagagagaat accttcagca gaaagctggc
    36001 gacgataacc gattttaggt tttttctcca ttatcgtttg aggtttaact gaagtggtat
    36061 ccacaccgac taacttatca gcttttccta gtgctgcgac aatttcggaa gcatagcccg
    36121 cagttgaaac aattttttct gccgcctttg tggtatttaa acacattgat agggcaatga
    36181 aagaaaaaag taaggttctc attttatact ccatttgatt tttatctaaa cttaaataa
//
LOCUS       DANLYI010000005        31412 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.005, whole genome shotgun sequence.
ACCESSION   DANLYI010000005 DANLYI010000000
VERSION     DANLYI010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 31412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31412
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.005"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            complement(<1..928)
                     /gene="rbsC"
                     /locus_tag="PXD81_000181"
     CDS             complement(<1..928)
                     /gene="rbsC"
                     /locus_tag="PXD81_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005753457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HDL5536255.1"
                     /translation="MATQTKQFQLGRFLIEQRSFVALFILIVSVSMINPDFFSVDNIL
                     NILRQTSVNAIIAVGMTFVILIAGIDLSVGSVLALTGAVAATLVGSELPILLVIPLVL
                     LLGTAFGGISGAIVAKGKVQAFIATLVTMTLLRGITMVYTDGRPISTGFSDQADAFAF
                     IGTGYLFGIPVPIWIMAVVFGLAWYILKHTPIGRYIYALGGNESATQLSGINVNKIKI
                     FVFAVSGFLSALAGLIVTSRLSSAQPTAGVSYELDAIAAVVVGGTSLMGGKGRVMGTL
                     VGALIIGFLNNALNLLDISSYYQMIAKALVILA"
     gene            complement(938..2434)
                     /gene="rbsA"
                     /locus_tag="PXD81_000182"
     CDS             complement(938..2434)
                     /gene="rbsA"
                     /locus_tag="PXD81_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
                     /protein_id="HDL5536256.1"
                     /translation="METLLKMSGIDKEFPGVKALSNACLSVYGGRVMALMGENGAGKS
                     TLMKVLTGIYSKDAGSISYLGKEVTFKGPKDSQEAGISIIHQELNLVGNLTIAENIFL
                     GRESKTAWGAIDWKKMYAEADKLLARLGVKHSSHQLCAELSIGEQQMVEIAKALSFES
                     QVIIMDEPTDALTDTETASLFKVIRELKAENRGIVYISHRLKEIFEICDDVTVLRDGQ
                     FIGERVIADIDEDQLIEMMVGRRLEEQYPHLHQERGELLLEVKNLSGSGVNNVSFQLH
                     KGEIVGISGLMGAGRTELMKVLYGALPKTAGSIELNGKAISNHCPQDGLNNGIVYISE
                     DRKGDGLILGMSVKENMSLTSLDHFSKAGSIRHDAEKLAVDDFIEMFNIKTPSREQQI
                     GLLSGGNQQKVAIAKGLMTRPNVLILDEPTRGVDVGAKKEIYQLINEFKKDGLSILMV
                     SSDMPEVLGMSDRILVMHEGQISAEFSRAEATQEKLLAAAIGKSTGLN"
     gene            complement(2444..2863)
                     /gene="rbsD"
                     /locus_tag="PXD81_000183"
     CDS             complement(2444..2863)
                     /gene="rbsD"
                     /locus_tag="PXD81_000183"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005753460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HDL5536257.1"
                     /translation="MKKTLNLNAQLSHSIATLGHTDSLTVCDAGLPIPNTIERIDLAL
                     TAGVPSFLQTFDVVVSELFVERVLLAEEIKHKNPQVLAALLERLTQLEQTQQNKIQID
                     YVNHEIFKEQTHYSKAVVRSGECSPYANIILYSGVPF"
     gene            3093..3353
                     /locus_tag="PXD81_000184"
     CDS             3093..3353
                     /locus_tag="PXD81_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhL family 4Fe-4S dicluster ferredoxin"
                     /protein_id="HDL5536258.1"
                     /translation="MALLITHKCTNCDMCLPECPNEAISVGDDIYIIDPVLCTECVGH
                     YEKPTCQKVCPITNCIIKDPDNIETEEQLWERFVMIHHPDQI"
     gene            complement(3385..4275)
                     /locus_tag="PXD81_000185"
     CDS             complement(3385..4275)
                     /locus_tag="PXD81_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate-binding protein YgfZ"
                     /protein_id="HDL5536259.1"
                     /translation="MAMNFKQVSQSYPNLCVPLSQYCLIEIAGVDAEKYLQGQLTCDV
                     AKISVGEHTLTSHCDPKGKMSALLRLYRAENEKFMAIIHRSLLPEALTQLKKYAVFSK
                     VTFTELDTPLYGMAGEAAFAKLSENMTALRLTTGQPRAIVWGEELETTADEQLWTLMD
                     IQDGIPVLLQQNQFELIPQATNLQALESAISFTKGCYIGQETVARAKYRGANKRALFT
                     LAGNFESEISLPEVASAIEMQLGENWRATGTVLTSATDENTLWVQVVLNKDVEPNTKF
                     RANGVALEMVELPYSLAENE"
     gene            complement(4284..5147)
                     /locus_tag="PXD81_000186"
     CDS             complement(4284..5147)
                     /locus_tag="PXD81_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011272075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HDL5536260.1"
                     /translation="MIYSMTAFAHLEMKKEWGNAVWEIRSVNQRFLETYFRLPEQFRN
                     LEMTLRERLRSALTRGKVECSLRIDLSKTQNSEIALNKEYAAQVVQSLKWLKETANEG
                     EINLVDVLRFPGVVDNESQDLDQIAQDLLAGFEQILADFIAMRAREGENVQALILQRL
                     EAISVEAAKVQAQMPEILQWQKDRLQQRFDELNLQLDPQRLEQEMVLLAQRVDVAEEL
                     DRLQLHVKETTNILKKGGAVGRKLDFMMQELNRESNTLASKSINADVTNSAVELKVLI
                     EQMREQIQNLE"
     gene            5428..6144
                     /gene="rph"
                     /locus_tag="PXD81_000187"
     CDS             5428..6144
                     /gene="rph"
                     /locus_tag="PXD81_000187"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HDL5536261.1"
                     /translation="MRPNNRANNQVREIKITRNYTRYAEGSVLIEFGETKVLCNATVE
                     ESVPRFLKGQQQGWVTAEYGMLPRATHSRTQREAAKGKQGGRTMEIQRLIARSLRAVV
                     DLKALGERTVTVDCDVIQADGGTRTASITGACVALYDAMNKLVANGTLKTNPMKGLVA
                     AISVGIVAGEAVCDLEYLEDSNAETDMNVVMVEDGRLVEVQGTAEGEPFSHQELLTLL
                     DLAKQGISQLVEAQRKALAE"
     gene            6368..8667
                     /locus_tag="PXD81_000188"
                     /pseudo
     CDS             6368..8667
                     /locus_tag="PXD81_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005759321.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
     gene            complement(8745..9446)
                     /locus_tag="PXD81_000189"
     CDS             complement(8745..9446)
                     /locus_tag="PXD81_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005614693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HDL5536262.1"
                     /translation="MDLQNRENFLNKLAQRMGRPRQNVPEPIPVLENNHAITRLTDRP
                     QPQLCQEFIDFARVMMADVVEAKEADLPQAIIDICEKYGGASVIVNNDQRLKDLGITA
                     AIQAKYEDSYVWDFNQAETNFEKARTANIGVVYGEYGLAESGGIVLFSDKEFGRSVSL
                     LPEKSVVVLRKSTVLPRVAQLAQILHEKAQRGERMPSCVNIISGPSATADIELIKVVG
                     VHGPVNKIYLVIDDL"
     gene            complement(9451..10860)
                     /locus_tag="PXD81_000190"
     CDS             complement(9451..10860)
                     /locus_tag="PXD81_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster-binding protein"
                     /protein_id="HDL5536263.1"
                     /translation="MSYLQTNTLPFKQRVEQQVNNEIVRKALVKAQETIGANRQRMVD
                     ELGNWEEWRDDAKAIRNHVLANLDAYLYQLSEKVTQNGGHVFFAETAEEATDYIKNVA
                     KAKNAKKIVKSKSMVTEEIGMNHVLEAEGIKVVETDLGEYLLQIVGDKPSHIVVPAIH
                     KDRHRIRQEMHDVLGYQGSETPEEMTAFVRQKIREDFLEADIGISGCNFAVPETGSVC
                     LVTNEGNLRMATTVPKTHIAVMGMERIAPTFKEVDVLITMLARSAVGAKLTAYNTWLT
                     GPRLEGETDGPEEFHLVIVDNGRSKILESEFQEVLRCIRCGACLNTCPAYRQIGGHGY
                     GSIYPGPIGSVISPLLGGYEEFKELPYACSLCTACNSVCPVKIPLAQLILKHREKIAD
                     AGLTPMAERLSIFGFNFANSHPTLWKVGVKVGAKVASKLIKNGKAPVNFGALGEWTKA
                     RDLPTAEGESFREWFNNRG"
     gene            complement(10862..11593)
                     /locus_tag="PXD81_000191"
     CDS             complement(10862..11593)
                     /locus_tag="PXD81_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HDL5536264.1"
                     /translation="MNVNFYVTCIADVMKAGVAKNTVLLLEKLGCKITFLEKQGCCGQ
                     PAINSGYTKQALAGMKNLVETFEANDYPIVAPAGSCVYAIKTYPDHFERFGEKEWAER
                     AKKVADRFYDLTDFIVNKLGVKDVGAYLPGKAVYHPSCSLFRKLGIKDEPIALLQHVK
                     GLELLPIKNQETCCGFGGTFSVKMAEISGEMVKEKVANVDTDEPDYLIGADVSCLMNI
                     GGRLSREGKPIKVMHIAEVLMQGGK"
     gene            complement(11737..13341)
                     /locus_tag="PXD81_000192"
     CDS             complement(11737..13341)
                     /locus_tag="PXD81_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005818537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HDL5536265.1"
                     /translation="MALFLSVFPIVLLIYLMVKRNALPSYVALPLTALLIFVLQLTYF
                     GNDTTLIFANIIAGLGDVLTPITVIFGAILFNRFSEVSGATNTMRKWLGTINPNPVAQ
                     LMIIGWAFAFMIEGASGFGTPAAIAAPILVGLGFKPLQVAMLALVMNSVPVSFGAVGT
                     PTWFGMGPLLKDGLLTDAQVLEIGSITALIHSIAAFIIPVMALRLIVSWKEIRQNIVF
                     IYISIFACVIPYFIIAQFNYEFPSLVAGAIGLLVSVWVANMGIGLAKSENHLDGDKAT
                     FGEVAKALLPTGLLIFILVITRIQQLPLKAMLNDATAWIVSSLGFANFEISQGLIFAL
                     KNILGTNVATSYKLLYVPALIPFVVTVLICLPIFSVSGKNTQEIFGTSFKQVKKPFLA
                     LLGAIVMVKLMLIGGVDSMVQIIGKNFAEFTGQSWTLFASFLGAIGAFFSGSNTVSNL
                     TFGTVQYATAQQVGLSVTLILALQSVGGAMGNMVCINNIVAVCSVTGIEKQEGNIIKK
                     TAVPMFVYGVIAAISALFIVPMFYNL"
     gene            complement(13631..14722)
                     /gene="ychF"
                     /locus_tag="PXD81_000193"
     CDS             complement(13631..14722)
                     /gene="ychF"
                     /locus_tag="PXD81_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HDL5536266.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANYPFCTIEPNTGVV
                     PMPDPRLDALAEIVKPERVLPTTMEFVDIAGLVAGASKGEGLGNKFLANIRETDAIGH
                     VVRCFENDDIVHVAGKIDPADDIEIINTELALADLDSCERAIQRLQKRAKGGDKDAKF
                     ELSIMEKILPVLENAGMIRSVDLDKDELHAIKGYNFLTLKPTMYIANVNEDGFENNPY
                     LDRVREIAEKEGAVVVPVCAAIESEIAELDDEEKVEFLQDLGIEEPGLNRVIRAGYRL
                     LNLQTYFTAGVKEVRAWTVSVGATAPKAAAVIHTDFEKGFIRAEVIAYQDFIDNKGEA
                     GAKEAGKWRLEGKDYIVQDGDVMHFRFNV"
     gene            complement(14908..15495)
                     /gene="pth"
                     /locus_tag="PXD81_000194"
     CDS             complement(14908..15495)
                     /gene="pth"
                     /locus_tag="PXD81_000194"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HDL5536267.1"
                     /translation="MSEIKLIVGLANPGVKYEETRHNAGEWLINELARIYNVSLKEEP
                     KYFGKVAKINTAQGEVRLLVPTTFMNLSGKAVGALANFYRIKPEEILVAHDELDLPPG
                     SVKLKKGGGHGGHNGLKDIIAALGNNKEFYRVRIGIGHPGHKDLVANYVLGKPSPSDQ
                     TLLNSAVDEAARCVEVLFKDGMVKATNRLNSFKAN"
     gene            complement(15568..16443)
                     /locus_tag="PXD81_000195"
     CDS             complement(15568..16443)
                     /locus_tag="PXD81_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HDL5536268.1"
                     /translation="MMKKFIPVMIAALVLSACSSQPAKRKSTPRIKATAAYIPTENSA
                     YVVFDLNQKKFLEGKNFNLVRPIASVTKLMTAVVFLEENRYGASCQTQILPSDADFLK
                     GTTSPLPKNLNIACSELLKAMLVRSDNYAAHALAHATHLNKAQFLARMNSKAREIGMT
                     QTYFGDSSGLSSDNVSTVVDLAKLAGYAASKPEIQQLSNSPLVSVYAGGRYFSMKNTN
                     SMVRSQSYQALVSKTGYTREAGYNLAFIAKGDCRGKRIGVVSLNNDNAASRTDFTESM
                     LAKYQCVPNPYRQYD"
     gene            16665..18245
                     /gene="prfC"
                     /locus_tag="PXD81_000196"
     CDS             16665..18245
                     /gene="prfC"
                     /locus_tag="PXD81_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014326123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HDL5536269.1"
                     /translation="MSYPQEVNKRRTFAIISHPDAGKTTITEKVLLYGNAIQTAGSVK
                     GKGSAQHAKSDWMEMEKQRGISITTSVMQFPYNDCLVNLLDTPGHEDFSEDTYRTLTA
                     VDSCLMVIDSAKGVEERTIKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVESVLK
                     IKCAPITWPIGCGKLFKGVYHLYKDETYLYQTGQGHTIQEVRVVKGLNNPELDQAVGD
                     DLAQQLRDELELVQGASHEFDHTEFIKGELTPVFFGTALGNFGVNHFLDGLTEWAPAP
                     QARQSDEREVSPSEEKFSGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLKHVRIG
                     KDVVISDALTFMAGDRSQAEEAYAGDIIGLHNHGTIQIGDTFTQGENFKFTGIPNFAP
                     ELFRRIRLRDPLKQKQLLKGLVQLSEEGAVQVFRPLINNDLIVGAVGVLQFDVVVSRL
                     KTEYGVEAIYETVNVATARWVEGKDAKKFEEFKRKNEANLALDGGDNLTYIAPTMVNL
                     NLAQERYPDVQFFKTREH"
     gene            18504..20078
                     /locus_tag="PXD81_000197"
     CDS             18504..20078
                     /locus_tag="PXD81_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009874733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL5536270.1"
                     /translation="MKKWLIPLTVAAFCVSACDDKNSTQATAEKPQVENTQSQKNAEI
                     LPLATVVAPWEINSLDPNQSGVIFQRMNLAETLVEADLNGNLIPALATDWRSNEEGTI
                     WRFTLREGVKFHNDQVLTAQAVVNSLNIAKSKPGVLAKAFIKEIRAVDNKVEIELTKA
                     FVPFPPFLTHYTALILAPESYNEKGEVTQIIGTGAFKPTKIEAPQKLEAVQFEGYWGK
                     KPQVQQANYLASSRSETRMLMAQSEPSSLVFNLDTASVARLKADPNLQLVSGSIARTI
                     QLKMDVAKPFFNDLAIRQALSQAINRKAIAEQVLKIENGAADQILPKAFADWRVDTAE
                     QVVDIAKIKQNLTASGYTFDEQGRLTKDGKPFSFTMRTFSDRPELPVIATILQAQWKQ
                     IGVEVNVSVGNFSEIPAGHQDGSLEMALYALNYGKTLDPYGVIASDFANGGSDWGVMN
                     WQNTEVDNAVKSLETERDPQKAKALKQTVSKIIYNDLPIIPVVYYQQNVVAHKEVKNV
                     TLDPFERRFFLELLSK"
     gene            20298..21230
                     /locus_tag="PXD81_000198"
     CDS             20298..21230
                     /locus_tag="PXD81_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL5536271.1"
                     /translation="MHKILFRRLIQILLVIWSVGTLTFILTRQLSGDMAYRIAASRYG
                     YDQADSAAAEAVRAELGLDQPWWQSYFSWLSDLAQFNLGKSLVTGDPVWSEIQHQFGH
                     TLSLAIVALILAILIGPPLGLLAARKPNGFFDRLTLVFSTLFRSVPAFIIAIGLITLF
                     SAQLKWLPAGGYGCWQHFILPALTLAIGLSTVSVRVTRSAMVQVKGSEFYQFSQLKGL
                     SRWHTFLRHGLPNIAVPVIAYHAVQLVYLIEGVVVVESLFAWPGSGHALVHAIIARDV
                     PMIQGTTLVMGGLFVVLNTIADLLSAWIDPRIKR"
     gene            21240..22022
                     /locus_tag="PXD81_000199"
     CDS             21240..22022
                     /locus_tag="PXD81_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL5536272.1"
                     /translation="MKISISQYLGGTILLAMLGFAFLQPYFYPMDIGFQDLTNILSNP
                     NEIAWFGTDHLGRDMLARLASAIRLSFGLSLFSVICALIAGLITGIMAGFFGGWIDRI
                     FSFICDLVMALPGLLLVLLFAALSPGSFWTLYLGISLVMWVEFFRVIRSISSTMANSS
                     EIQASRLMGMGWFYSFKRHLLPRLLPMIATLSAFSLGNAILALATLGFVSVGLRPPTA
                     ELGLMMTELFPYYYEAPLIFLQPVVAVFLMVLSLQLLSRKIK"
     gene            22111..23523
                     /locus_tag="PXD81_000200"
     CDS             22111..23523
                     /locus_tag="PXD81_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL5536273.1"
                     /translation="MKLLDIQNLSVETENRLQLVEPISLQLKAGKNITILGETGSGKS
                     LLIQAIMGALPDGLIASGEISVKNCKISTESDRLQTLWGKTLVMLPQEPRRSLNPIMA
                     IGKQLWESFHYVAGKPSSQAKTESKAYLAKLGLGESVENYPHQLSGGMAQRASFAIAT
                     AAGGKILLADEPTKGLDPISKGKVIELLKSAYQNGGGLLTITHDIEVAEQLGGTILVM
                     KKGELLEQGEAKALLNNPQHPYTKALISADPKHWQPLQDLQNLSQKQPLVSVKNLSIA
                     RGKKTLFSNLSFDLHKGEVLGIVGHSGIGKSSLADVLCGLLKPKSGEVVWHSHSHKKH
                     QVLKLYQDPPEAFAPNVSLKTLLDDVINKHQLDRLPIPSLLQQLSLNPEILQRNAENV
                     SGGELQRVAILRALLLEPVLLFADEVTSRLDPITQQETIELLINQCRARNCSLILVSH
                     DPYLIEKSCDKVINLEEYVG"
     gene            complement(23610..24353)
                     /gene="rlmB"
                     /locus_tag="PXD81_000201"
     CDS             complement(23610..24353)
                     /gene="rlmB"
                     /locus_tag="PXD81_000201"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014992094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HDL5536274.1"
                     /translation="MSEQIYGIHAVKAFLDNAPERLIEVLVLKGREDKRLMPLLNELQ
                     RLGVAIQQVNRQTLDNKSQGEVHQGIIARVVPQKELNEHDLEAILSQKKNPLLLILDG
                     VTDPHNLGACLRTADAAGVDAVIVPKDKSAQLTPTARKVACGAAEVMPLIRVTNLART
                     MRDLQERHNVWIVGTAGEAESGIYEAKLTGSIALVMGAEGDGMRRLTREHCDQLISIP
                     MAGSVSSLNVSVATGVCLFEIVRQKLAGQ"
     gene            complement(24408..25775)
                     /gene="pssA"
                     /locus_tag="PXD81_000202"
     CDS             complement(24408..25775)
                     /gene="pssA"
                     /locus_tag="PXD81_000202"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005616526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HDL5536275.1"
                     /translation="MLILNKQRKAEYLLKSLPYLPQVAEQVQFLASSKAFKQQILQLI
                     QTAKKRIYITALYFEKDEAGQEVLAALYQAKRNNPELEINILVDWHRAQRGRIGEAEN
                     LSNADWYAKVKYEQGLPAGQEIAFYGVSINGREVFGVLHLKGFVFDDTILYSGASINN
                     VYLHQFERYRYDRYHVIENKVLADSWVTFIQQHLLTNEAVKRLDTENRPKTAEIRAQI
                     KHFRKQLSREAYLFNGVAENGRLAVSPLVGLGRRKNQLNKVIEALFYQTEQKLTICTP
                     YFNFPHSLRTRIEWLLANGKSVEIIVGDKTANDFYTKPDEKFTMASALPYLYEKNLRA
                     FAKRLDGYVQNKQLTIRLWKDGENSYHLKGVWVDNRYILLTGNNLNPRAWRLDAENAI
                     LISDPQHQLSEKAETELNQICQHTQILTHYSDLEVLTDYPENVRKLLKKFGRVKLDKI
                     VKMLL"
     gene            complement(25845..27392)
                     /locus_tag="PXD81_000203"
     CDS             complement(25845..27392)
                     /locus_tag="PXD81_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tannase/feruloyl esterase family alpha/beta
                     hydrolase"
                     /protein_id="HDL5536276.1"
                     /translation="MMKIIAFLFIFLSVFSHTALANSMAEQCVALKSNSPEKTAIYKA
                     EWVEKSAELPAYCLVQGEIGKRVEVDNVPYGIQFELRLPEQWNEKFLFQGAGGVGGVI
                     FPAIGKLYPHGASGANGLNRGYAVVSNDSGHATRDLVFTADPEARSNYAYASIGKVTT
                     VAKQLIAEFYRKPSKHNYIMGCSNGGREAMMAAMRYPQEFDGVIAGSPGFRVSRSVLA
                     EVWDNRALLAVAPKNGDGDKILSQALTQQDLDVIANGVLTRCDKLDGLADGLINAWEQ
                     CDFQPEMVAKQLGQKKVALIKTIFEGAKNSRGELIYSPWAYDSGINAKGWRDWKLGDS
                     QTAQPNSISFKMGLKSLTHYYLAPRVPSRDPLSVDLDQASEQVKAVGSIHDTDQVDLS
                     AFKQNGGKMLIYQGVSDPIFSAVDLKNWYQSLQQAVENPQHFAKLFFVSAMNHCGRGA
                     TVNDFDMLTALENWVEKGLEPESIAAKAGELYPNKAKQIPLCAYPKVAIYQTGEDPNR
                     LTSYQCR"
     gene            complement(27517..30333)
                     /gene="ileS"
                     /locus_tag="PXD81_000204"
     CDS             complement(27517..30333)
                     /gene="ileS"
                     /locus_tag="PXD81_000204"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011608339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HDL5536277.1"
                     /translation="MTIDYKNTLNLPETGFPMRGDLAKREPDMLKNWYDKNLYQKIRT
                     ASKGKKTFILHDGPPYANGNIHLGHAVNKILKDMILKSKTALGFDTPYVPGWDCHGLP
                     IELKVEGIVGKPNEKISAAEFRQACRDYAKEQVEGQKADFIRMGILGDWDNPYLTMNF
                     NTEANIIRTLGKVIANGHLYKGSKPVHWCLDCGSSLAEAEVEYEDKVSPSIYVRFNAV
                     DVNAVEEKFNAQGKGRGALSAVIWTTTPWTIPSNRAIAINPELDYALVQLGDERVLLA
                     VDLVEDVAKAAGVESAEILATTKGENLELLRFNHPFYDYSVPFIFGDHVTTDGGTGLV
                     HTAPDHGADDFVVARKYNIEMAGLISNDGKFKADTPFFAGLGVFESNDKVVEKLQEVG
                     ALLKLSRIKHSYPHCWRHKTPIIFRATPQWFIGMETQGLRQQALGEIKSVRWIPSWGE
                     ARIDTMVANRPDWCISRQRTWGVPMAMFVHNETEELHPRTLELIEEVAKRVEEKGIQA
                     WWDLDPAELLGEEAKDYRKVPDTLDVWFDSGSTYASVVEQRPEFNGNSADMYLEGSDQ
                     HRGWFMSSLMLSTATNGKAPYKQVLTHGFTVDEKGRKMSKSLGNVIVPSEVWNKNGAD
                     ILRLWVASTDYTGEITVSHNILNSAGESYRRIRNTARFLLANLNGFDPKSDLVQPQEM
                     IALDRWAVACALEAQNDIKEAYDNYQFHTVVQRLMRFCSIEMGSFYLDIIKDRQYTTK
                     ADSLARRSCQTALWHIAEALVRWIAPILSFTADEIWGYLPQVEGRSEFVFTEEFYTGL
                     FGLTEQDKLDDAYWQQILKVRAEVNRVLEQARKDKVIGAGLEAKVTVFANDEIRPLLE
                     QLGNELRFVLITSQAIVKPLAEADIAEGELAGLAVKVENADGEKCPRCWHYATDIGSH
                     SGHEEVCGRCVENVAGEGEKRLFA"
     gene            complement(30436..31398)
                     /gene="ribF"
                     /locus_tag="PXD81_000205"
     CDS             complement(30436..31398)
                     /gene="ribF"
                     /locus_tag="PXD81_000205"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HDL5536278.1"
                     /translation="MQLIRGFHNLDKHIALNQGCVLSIGNFDGVHLGHQNILARLCDR
                     ALDLGLPSVVMLFEPQPREFFAKKSGNSTACNPPARLMRLRDKLKYLEEAGVDFVLCV
                     RFSEKFAQISADDFISDLLAKRLKVRYLSVGDDFRFGAKRAGSFETLRNAGLKYRFAV
                     EESHTHSLNEERISSSLVREALQNNELDLAEKLLGKPYSILGRVAHGNKLGRTIGFPT
                     ANIMLNRLVTPIQGVFAVKVKTKQGSFNGIANVGNRPTINGTKPLLEVHIFDFNASIY
                     GEAIEVIFLKKLRNEIKFPDFEALKAQIEKDRLQAVEFFKEFAR"
BASE COUNT         8969 a         7231 c         6185 g         9027 t
ORIGIN      
        1 ctgctaaaat aaccaacgct ttagcaatca tctgataata tgaagaaata tccaataaat
       61 taagggcatt gtttaagaaa ccgataatta atgccccgac aagcgttccc attacacgcc
      121 ctttaccgcc cattagactg gttccaccaa caactaccgc tgcaattgcg tctaactcat
      181 aggacacacc tgccgttggc tgagcagaag agagacgtga agtcacaatt aagccggcaa
      241 gagcagataa aaaaccactc acggcaaata cgaagatctt aattttattc acattgatgc
      301 ctgaaagctg ggttgccgat tcgttgccac ctaaagcgta aatgtaacgc ccaatcgggg
      361 tgtgtttcaa aatataccaa gctaacccaa acactaccgc cataatccaa atcggcacag
      421 gaataccaaa taaatagcct gtcccaataa acgcaaaggc gtctgcttgg tcagaaaagc
      481 cggtgctaat cggtctgcca tcggtataaa ccatcgtaat cccacgcaat aaggtcattg
      541 tgaccaaagt cgcaataaat gcctgcactt tacctttagc aacaatcgcc ccactaatac
      601 caccaaatgc cgtccctaat aataaaacca gcggaatcac caacaaaatc ggcaattcag
      661 aaccgactaa ggtcgctgca accgcaccgg ttaaggctaa aaccgaaccg accgataaat
      721 caatgcctgc aattaaaatc acaaaggtca ttccgaccgc aataatcgca ttcaccgagg
      781 tttggcgtag aatatttaaa atattatcca cgctgaaaaa atcagggtta atcattgaaa
      841 cactgacaat taaaataaat aacgctacaa aagaacgttg ctcgattaaa aatctgccaa
      901 gttgaaactg ctttgtttga gttgccataa acacacctta atttaatcct gtacttttgc
      961 caatagctgc tgccagtagc ttttcttgtg tcgcttctgc tcgtgaaaat tctgcactga
     1021 tttgcccttc gtgcatcact aaaatgcggt cgctcattcc caacacttcc ggcatatcgg
     1081 acgaaaccat caaaatacta agcccatctt ttttaaattc attaattaat tgatagatct
     1141 cttttttcgc ccccacatca acaccccgtg tcggctcgtc taaaatcagc acattcgggc
     1201 gggtcatcaa ccctttagcg atcgccactt tttgctgatt accaccggaa agcagcccga
     1261 tttgttgctc ccgacttggg gttttaatgt taaacatctc aataaaatca tcgaccgcca
     1321 atttttctgc atcgtggcga atactgccgg ctttggagaa atgatcgaga gaggtcaaag
     1381 acatattttc ttttaccgac attcccaaaa tcagaccatc gcctttacga tcttctgaaa
     1441 tatacacaat gccgttattc aacccatctt gcggacaatg gttggaaatc gcttttccat
     1501 ttaactctat cgagccggct gttttcggca atgcaccata aagcaccttc attaattctg
     1561 tacgccctgc ccccattaag ccggaaatac ccacaatttc acctttgtgt aattggaatg
     1621 acacattatt caccccactt ccacttaaat tcttcacttc aagtaataac tcgccacgct
     1681 cttggtgtaa atgcggatat tgctcttcca aacggcgacc aaccatcatt tcaattaact
     1741 gatcttcatc aatatcggca attactcgct cgccgataaa ttgcccgtct cttagcaccg
     1801 tcacatcatc acaaatttca aaaatctctt ttaaacgatg ggaaatataa acaatgcctc
     1861 gattttccgc ttttaactct cgaataactt taaacaacga tgctgtttct gtatcggtta
     1921 aagcatcggt cggttcgtcc ataataatta cttgagactc aaaactcagg gctttcgcaa
     1981 tttccaccat ttgctgctcg ccaatggata actccgcaca aagctgatgg ctactgtgtt
     2041 ttaccccaag tcgggcaagc agtttatccg cctcggcata catttttttc caatcaatag
     2101 ctccccaagc ggttttggac tcacggccca aaaaaatatt ttcagcaatg gttaaattac
     2161 cgactaaatt cagctcttgg tgaataatgc taataccggc ttcttgtgaa tctttcgggc
     2221 ctttaaaggt aacttctttg cctaaatagc taatagagcc ggcatctttg ctataaatcc
     2281 ccgtcaatac tttcatcaag gtggatttac ctgccccatt ttcgcccatt aatgccatta
     2341 ctcgcccacc gtaaacagat aagcacgcat tgctcaaggc tttcacgccg ggaaattctt
     2401 tatctatccc gctcattttt aataaggttt ccatcattcc accctaaaac ggcacgcccg
     2461 aatataaaat aatattggca tacggtgaac actcacccga acgcacaaca gctttactgt
     2521 aatgtgtctg ctctttaaag atttcgtggt tcacataatc aatttgaatt ttattctgtt
     2581 gagtttgttc taattgggtt aaacgctcca aaagagctgc taacacttgc ggatttttat
     2641 gtttaatttc ttcagcaagt agcactcgtt caacaaataa ttcactaact actacatcaa
     2701 atgtttgtaa aaaacttggc acaccggcgg ttaaagccaa atcgatccgc tctattgtgt
     2761 ttggaatcgg caaaccggca tcgcaaaccg ttaaactatc ggtatgcccg agtgtcgcaa
     2821 tactgtgcga aagttgtgca tttagattta gcgttttttt catactaacc cctttatttt
     2881 cgctccatca cgttggaaaa tactccatcg aaacgtttcg atggcatcat aaaaaatttt
     2941 cttaatcgga taaacaacca aattgcaaaa atgtgaagta gttcaaatta ttttgcttta
     3001 taataggtag catttagtga caagcggtaa gatttgcaaa tttttttgca aaatcgaccg
     3061 cttgtttaat ttttattttc tcaatataaa ctatggcttt attaattacc cacaaatgta
     3121 ccaactgtga tatgtgcttg ccggaatgcc cgaacgaggc gatttcggtg ggcgatgata
     3181 tttatatcat cgatccggtg ttatgtaccg aatgtgtcgg gcattatgaa aagccgactt
     3241 gccagaaagt ttgcccgatt accaactgca ttatcaaaga tcctgataat atcgaaaccg
     3301 aagagcagtt gtgggaacgt tttgtgatga ttcatcaccc tgatcagata taaaaaaacg
     3361 gtcataccct gatgaccgtt cgttttactc attttctgcc aaactataag gtaactcgac
     3421 catttccaac gctacaccat tggctctaaa cttggtgtta ggttcaacat ctttattcag
     3481 caccacttgc acccacaggg tattttcatc agtcgcactg gtcaacacag tgccggttgc
     3541 tcgccaattt tcgcccagtt gcatttcaat cgcagaagca acttccggta agctgatttc
     3601 gctttcaaag ttgccggcaa gggtaaataa agcacgttta ttcgccccac gatatttcgc
     3661 tctcgctacc gtttcttgcc cgatgtagca acctttggtg aatgaaatcg cagattcaag
     3721 tgcctgtaaa ttggttgcct gtggaatcag ctcaaactgg ttttgttgca agagaacagg
     3781 aatgccgtct tgaatatcca ttaatgtcca gagctgttca tcggcagtcg tttcaagctc
     3841 ttcgccccaa acaatggccc gaggctgtcc tgtggttaaa cgcaaagcgg tcatattttc
     3901 gcttaatttt gcaaatgctg cttcacctgc cataccataa agtggggtgt ctaattccgt
     3961 gaaggtcact tttgaaaaaa ccgcatattt tttaagttgc gtcagggctt caggcaataa
     4021 gctgcggtga atgatcgcca taaatttctc attttctgca cgatacaaac gcagtaacgc
     4081 actcattttg cctttcggat cacaatggct ggttagcgta tgttcgccca ccgaaatttt
     4141 tgccacatcg caggttaatt gcccttgaag atatttctca gcatctaccc ctgcgatttc
     4201 aattaaacaa tactgagaaa gcggcacgca gagatttgga tagctctggc tcacttgctt
     4261 gaaattcatt gccataatct gtcctattct aaattctgaa tttgttcacg catctgttcg
     4321 atcaacactt tcagctccac cgccgaattg gtcacgtccg cattaatcga ttttgaggcg
     4381 agcgtgttgg attcacggtt tagttcttgc atcataaaat ccagtttacg cccaaccgca
     4441 ccgccttttt tcaggatatt ggtggtttct ttaacgtgta attgcaagcg gtctaattcc
     4501 tccgccacat caacacgttg ggcaagtaac accatttctt gttctaagcg ttgtggatca
     4561 agctgtaaat tcagttcatc gaaccgttgt tgtaaacgat ctttctgcca ttgcagaatt
     4621 tccggcattt gggcttgtac ctttgcagcc tcaactgaaa ttgcttctaa acgttgtaag
     4681 atcagggctt gcacgttctc gccctctctg gctcgcattg cgataaaatc tgccagaatt
     4741 tgttcaaagc cggctaataa atcttgagca atttggtcta aatcttggct ttcattatcc
     4801 accacgcccg ggaaacgtag cacatcaaca aggttgattt cgccttcatt tgcggtttct
     4861 tttaaccatt ttaatgattg aaccacctgt gcggcatatt ctttgtttaa cgcaatttca
     4921 ctgttttggg ttttgcttaa atcaatgcgt aagctacact ctactttgcc tcgtgtcagt
     4981 gcagaacgta aacgctcacg caaggtcatt tctaggttgc gaaactgctc cggcaaacga
     5041 aaataggttt ctaaaaagcg ttgatttacc gagcgaattt cccacaccgc attgccccac
     5101 tcttttttca tttctaaatg ggcaaatgct gtcatactat aaatcattgg tattccttgt
     5161 gtttttataa gtaaagctct ctccccttga gggagagaga ccgaaaaata tttcgttcag
     5221 aaatattttt cagagagagg gaggaatttg taaaatttga tataaaaata accgcttacc
     5281 cctctccctg atttttcttc tgaacgaaga aaaatccgtc cctctcccac aaggggcgag
     5341 ggctatgtaa attctttgct aaagaaaaat cacattttac tgtgttcttt gctaaaatgg
     5401 taaaaatttt agcaaaagga aaagaaaatg cgtccaaata atcgagcaaa caaccaagtt
     5461 agagaaatta aaattacccg taactacact cgctatgccg aaggctcggt attaattgag
     5521 tttggcgaaa ccaaagtatt gtgtaacgct acggtagaag aaagcgtgcc acgcttttta
     5581 aaaggtcagc aacaaggttg ggttaccgca gaatatggta tgttgccacg agccacccac
     5641 tctcgcactc agcgtgaagc ggcaaaaggc aaacaaggcg gacgcacaat ggaaatccaa
     5701 cgcttgattg cccgttctct gcgtgcggtg gtagatttaa aagccttagg cgaacgtacc
     5761 gttaccgtag attgtgatgt tattcaagcc gatggaggca ctcgcaccgc ctcaattacc
     5821 ggtgcgtgcg tagcattgta cgatgcaatg aataagttag tagctaatgg aacactaaaa
     5881 actaacccaa tgaaaggttt agtcgcagca atttctgtcg gtattgtagc aggcgaagcg
     5941 gtgtgcgatt tagaatatct ggaagattcc aacgctgaaa ctgatatgaa cgtggtgatg
     6001 gtcgaagatg gtcgtttagt ggaagtacaa ggcacggcag aaggcgaacc gttctcgcac
     6061 caagaattac tgacattgct agatttagcg aagcaaggca ttagccagct agttgaagct
     6121 caacgtaaag cgttagctga ataattcact tctctttttg attttctttt taaataaaat
     6181 ctacatctga caaaacagaa taaccgttct gattcagatg tagatttttt tatttccctt
     6241 ttcaaaatac actcaatcat aaaataacaa ttattttttt atggaaatat tgcacttctt
     6301 acaaaatatc tgtaaatttc attgccatta tttttttatg gctatttatt ctagaaggac
     6361 tctaaacgtg ttaaagaaaa attatctcac tgtatcaatt ctccttgcca ttagtggagt
     6421 aggctacgct aatgaaatct ctttagagac tattactgtt gatggaaata cgccttcaac
     6481 caaagggaaa ttactaggtg gtgaactaaa tagcaatgaa tctgttgttg atgaaaaaaa
     6541 ttaaaacaag gtagtattac attaggtaac gctttatctg gtgaattagg cattcattca
     6601 agccagttcg gaggcggtgc aagtacccca attattcgtg ggcaagagag taaacgtgca
     6661 aaaatcttac aaaataatgg cgaaaactta gatatgtcgg gaatgtctcc tgatcatgca
     6721 gtaaccgtag atgcgctact tgctaaacgt attgaaattt tacgtggtcc aactacttta
     6781 ctttacagtg caggtaatac ggcaggtgta attaatgtgg tagacaataa aattccaacc
     6841 gctattcccg aaaaaggcta tgaggggcaa tttggtgtgc gttttggctc agccagtaaa
     6901 gagcgtttaa cctatgcagg ctcaaccttt gcccttggaa atcatttagc cttgcgggta
     6961 cagggaatgt ataacaaagc ttccgaatac tatgctccac actttacgat tgaaggcaaa
     7021 ccttaccacc gcgtaccaga tagcgatgta caatcacaaa ccggcacagt aagcctttct
     7081 tggattggag agcgaggaca tcttggtatt gcttacactg atcgccgtga taaatatgga
     7141 ttaattggtc acacacacaa atacgatcac tatactatta gtattattcg ccaagccgtt
     7201 atgtttgcaa aaggttattt acgtttttat ccgcaccttg cagaagaagg agatattgac
     7261 tataacaacc ctggtatccg tttactgcat actcatattc cgggaggctc acattatgga
     7321 caagatactc acgagcacgg caaaccttgg attgatatgc actccaagcg gtatgatatt
     7381 gatggaagtt tgcaaaatcc acttcctggt tttgaagaag ccaaaatatc agctaactat
     7441 gtagattact atcatgatga aaaagatgga aaaagagttg aaaattactt caagaacaaa
     7501 ggaaaaaatc tacgtttcga attggttcac aaagagtgga aagggctaaa aggagctatt
     7561 ggtgtgcaat ataccaacca atctacctca gcacttgcac ttgaagcttc acgagctgca
     7621 aaagtattta ataaacagcc tttactcaat aatccaaaaa caaaattatg gagtctcttt
     7681 gcgattgaac gccttaattt aggtgacttt acctttgagt taagtgggcg ggcagaacgt
     7741 caaaaaatag caatggatta tgatgtgaaa ttaattgatc gctggctagg ctttaataca
     7801 cctatgccaa acttggaccc acataaagac aaaggttatt cttatagctt tgcaactcat
     7861 tggtatttcg caccaaacca taagttaaca ttaaatgctg cacatcaaga acgtttacct
     7921 aatgctcagg aactctatgc tcatggtaaa cacattgctt taaatgcatt tgaggctggc
     7981 aataaaaatc taaaaaaaga gcgttctaac caaattgagc tatcattagc ttatgttggc
     8041 gacaaatggg attataaatt aaatctctac cacacccgct atggcaacta tatttaccca
     8101 ttaactctta atgataatcg tggacctaaa tcttttaccg atgaatataa cttgaaagtg
     8161 aatagatatt atcaaggcga agctcgtttt agtggggcag aaggcgaaat tggttatcta
     8221 tttacaccaa attatcgctt agccgtattc ggagactatg tccgtggaaa gttagtcaac
     8281 ttacctaata ttgcaatgag ttacaacatt tggacaggtg aagttgacaa atgggcttct
     8341 cagccagaca tttctgcacc tcgtatacca ccactacgtt taggagcaag atttaatgct
     8401 gacttcaacc taaactggtc tggaatgtta gaatattacc gagtatttgc tcagaaaaaa
     8461 gtctcgaaat acgaacaagt tacccctggc catcatcaag taaaccttgg cgtaacctat
     8521 agtaatcatt ttaatcaaac agaataccaa gtattcctta aagtggataa cttgcttaat
     8581 cagaaaatgt accaacatgc ctcatatcta ccacatattc cacaaatggg tagaaatgca
     8641 atgcttggaa tgaatatcag tttttaattt gtaaatctct acaaaaatat aaccgcttgt
     8701 aaatttctat ctacaagcgg ttactttcag tggatttttt gcaattagag atcgtcaatt
     8761 accaagtaaa ttttatttac cgggccgtga acgccaacta ctttaatcaa ctcaatatcc
     8821 gcagtcgccg atgggcctga gatgatattc acacaagatg gcattctttc acctcgttgg
     8881 gctttttcgt gcagaatttg tgcaagctgt gccacacgag gtaacactgt acttttgcgt
     8941 aacacaacca ccgatttttc gggcaataag ctcactgaac gcccaaattc tttatcggaa
     9001 aacagtacaa taccgccaga ctctgccaag ccatattcac cgtaaaccac accgatatta
     9061 gcagttctgg ctttttcaaa gttggtttca gcttggttga aatcccacac atagctgtct
     9121 tcgtattttg cttgaatggc tgcggtaatc ccaagatcct ttaaacgttg atcgttattc
     9181 acgatcacac tcgccccgcc atatttttca caaatatcaa taatggcttg tggtaaatcc
     9241 gcctcttttg cctctactac atctgccatc atcactcggg caaaatcgat aaactcttgg
     9301 caaagttgtg gttgagggcg atcggttaaa cgggtaatcg cgtggttgtt ttccaacaca
     9361 ggaattggct caggcacatt ttggcgtggt ctgcccatac gctgtgccaa tttatttaag
     9421 aaattttcac gattttgtaa atccattttc ttatcctctg ttattaaacc attcacggaa
     9481 actttcgcct tcggcagtcg gtaaatcacg ggctttcgtc cattcaccta acgccccaaa
     9541 attcaccggt gctttgccgt ttttaatcaa tttgcttgcc actttcgccc ccacttttac
     9601 gccgactttc caaagggttg ggtgtgagtt agcgaagtta aagccaaaaa tagacaagcg
     9661 ttctgccatt ggcgttaagc cggcatccgc aattttctca cggtgtttta agataagctg
     9721 tgccagtgga attttcactg gacaaacact gttacaagcg gtacaaagtg agcaagcata
     9781 cggcaattct ttaaactctt cgtagccacc gagcaatggc gaaataactg aaccaatcgg
     9841 gcccggatag attgaaccat agccgtgacc gccaatttgg cgatacgccg ggcaagtgtt
     9901 caaacatgca ccacagcgga tacaacgcaa cacttcttgg aattcgcttt ctaagatttt
     9961 agaacgaccg ttatcgacaa tgaccaagtg gaactcttcc ggaccatcgg tttcgccttc
    10021 aagtctagga cctgtcagcc aagtgttgta agcggtgagt tttgccccta ccgcactgcg
    10081 agccagcatg gtaattaata catcaacctc tttaaaggtt ggggcgatac gttccatacc
    10141 cattactgca atatgcgttt tcggcacagt tgttgccata cgcagattac cttcgttggt
    10201 cactaaacaa accgagccgg tttccggcac cgcaaagtta cagcctgaaa taccgatgtc
    10261 tgcctctaag aaatcttcac ggattttttg gcggacgaat gcagtcatct cttcaggtgt
    10321 ttccgaacct tgataaccta gcacatcgtg catttcttgg cggattctgt ggcgatcttt
    10381 gtggattgca ggtacaacaa tatgagaagg tttatcgccc acaatttgca ataaatattc
    10441 gcccaaatcg gtttcaacca ctttaatgcc ttctgcttct aaaacgtggt tcatcccaat
    10501 ctcttcggtt accatcgact tcgattttac gattttcttc gcatttttag ctttcgccac
    10561 gtttttgatg taatcagtgg cttcttcggc ggtttcggca aagaaaacgt gtccgccgtt
    10621 ttgggtgact ttttcgctta attggtagag ataagcgtct aaattggcta aaacgtggtt
    10681 acgaattgct ttcgcatcat cacgccactc ttcccagttg cccagctcat ccaccatacg
    10741 ctgacggttt gcaccgattg tttcctgagc cttaactaag gctttacgca caatctcgtt
    10801 attaacttgc tgctcaacac gttgtttaaa cggcagggtg ttagtttgta aataagacat
    10861 cttattttcc tccctgcatt aacacttcgg caatgtgcat cactttgatt ggcttacctt
    10921 cacggcttaa acgaccgcca atgttcatca agcagcttac atctgccccg attaaataat
    10981 ccggctcatc ggtatctaca tttgcgactt tctcttttac catctcgccg gaaatttctg
    11041 ccattttgac cgagaatgtg ccaccaaaac cacagcaagt ttcttggttt ttgattggta
    11101 ataactctaa ccctttaacg tgctgtaata aagcaatagg ctcatcttta atccccagct
    11161 tacggaataa gctgcaagac gggtgataaa ctgctttgcc cggtaaataa gccccaacat
    11221 ctttcacgcc cagtttgttc acaataaaat cggttaaatc atagaaacga tcagccactt
    11281 tcttcgcacg ttctgcccat tctttttcgc caaagcgttc aaaatgatcc ggataggttt
    11341 tgatcgcata tacacaagaa cctgccgggg cgacaatcgg gtaatcatta gcttcaaagg
    11401 tttcaaccaa gtttttcata cctgctaaag cttgcttagt gtaaccgctg ttgatcgcag
    11461 gttgtccaca gcaaccttgt ttttctaaga aagtgatttt acaacctaat ttttccaata
    11521 aaagcacagt attttttgca acccctgctt tcattacatc tgcaatgcag gttacgtaaa
    11581 aattaacgtt cataaagaac tcccacagta aatataaata agaattaaac tcaaaaaatg
    11641 ccgttctaag acggcgaaag gaagaggtgg atttgcaaaa aatttgctaa atctcaccgc
    11701 ttcctttcta aatcaagtaa gctgattatt aactcattat aggttataga acataggtac
    11761 tataaacaat gccgaaatcg cagcaataac gccatacacg aacattggca cggctgtttt
    11821 cttaataata ttgccttctt gtttctcaat gccggtaaca gaacacacag ccacgatgtt
    11881 gttaatacac accatattcc ccattgcacc accaactgat tgcaatgcaa gaattaaggt
    11941 aacagataac ccgacttgct gtgccgttgc atattgtact gttccgaagg ttaagttaga
    12001 taccgtattt gaccctgaga agaacgcacc aattgcacct aagaacgagg caaataatgt
    12061 ccaagattga ccggtaaatt ctgcgaagtt tttaccaatg atctgaacca ttgagtctac
    12121 accaccaatt aacattaatt ttaccatcac aatggcacct aaaagtgcta agaatggttt
    12181 tttcacttgt ttaaatgaag tgccaaaaat ctcttgagta tttttacctg ataccgagaa
    12241 aatcggtaag caaattaata cggtcacaac aaatggaatc aacgcaggaa catacagtaa
    12301 tttatagcta gttgctacat tcgtacctaa aatatttttc agtgcaaaaa ttaagccttg
    12361 gctaatttca aagttagcga aaccaagcga actgacaatc caagctgttg catcatttag
    12421 cattgctttt aatggaagtt gctgaatacg ggtgattacc aaaatgaaga tcagtaaccc
    12481 tgttggtaat aatgcttttg ccacttcacc aaaggtcgct ttatcgccgt caaggtggtt
    12541 ctcgcttttc gctaaaccaa tacccatatt tgctacccag actgacacca ataaaccaat
    12601 cgccccggca actaatgatg ggaattcgta gttaaattga gcaataatga agtaaggaat
    12661 gacacaggca aaaatgctga tataaataaa cacgatattt tgacgaattt ctttccagct
    12721 cacaattaaa cgcaatgcca taactggaat aataaacgca gcaatagagt ggatcagtgc
    12781 agtaatagaa ccaatttcaa gcacttgtgc atcggttagt aaaccatctt ttaataatgg
    12841 tcccatacca aaccaagttg gtgtgccaac cgcaccaaat gacaccggca cagagttcat
    12901 tactaaggct aacattgcca cttgtaacgg tttaaagcct aaaccaacca gaattggagc
    12961 tgcaattgcc gccggcgtac cgaaacctga tgcgccttca atcataaatg caaatgccca
    13021 accgataatc atcaactgtg ccaccggatt cgggttaatt gtccctaacc atttacgcat
    13081 agtgttggtt gcacctgaaa cttcagagaa acggttaaat aaaatcgccc cgaaaataac
    13141 ggtaattggg gttaatacat cacctaaacc ggcaataata ttggcaaaga ttaacgtggt
    13201 atcattacca aaataggtaa gctgaagcac aaaaattaat aacgccgtaa gtggtaaagc
    13261 cacataagac ggtaacgcgt tacgttttac cattaaataa atgagcaaaa caattggaaa
    13321 tacgctcagg aatagagcca tactgcacct ctttatatga ttataaaatg acctaactgg
    13381 tcggataggt gcgaattgta ctcttaaaaa aaactaatgt aactaggttg ttacaatttt
    13441 gtgatctagc tcgcattttt aatgattgta tggttaaaaa agagccatat ttattttcat
    13501 ttaatactta atagttagcc ctatttaaga catttttacc gaatcgagca atatgcaatt
    13561 aattaacaaa aaaaggccgc ttgtagatca cagatcaaca agcggtcttt tttaggcaaa
    13621 aatttgcaaa ttaaacattg aaacggaagt gcattacatc gccgtcttga acaatataat
    13681 ctttaccttc caaacgccat ttacccgctt cttttgcacc ggcttcacct ttattgtcta
    13741 tgaagtcttg gtaagcaatt acttccgcac gaataaagcc tttctcaaag tcagtgtgaa
    13801 tcacggctgc tgcttttgga gcggttgcac ctaccgaaac cgtccacgca cgcacttctt
    13861 ttacccctgc ggtaaagtag gtttgtaagt taagtaaacg gtagcctgca cgaataacac
    13921 ggtttaagcc cggctcttca atgcctaaat cttgtagaaa ttccactttt tcttcgtcat
    13981 caagctcggc aatttccgat tcaatcgctg cacacaccgg tacaaccacc gccccttctt
    14041 tttcggcaat ttcacgcaca cggtcaagat aagggttatt ttcaaaaccg tcttcgttca
    14101 cgttggcaat atacattgtc ggttttaaag ttaagaagtt gtagccctta atagcgtgca
    14161 gttcatcctt atctaaatct accgaacgaa tcataccggc attttcaagc acaggtagga
    14221 ttttttccat aatcgataat tcaaattttg cgtctttatc gccacctttc gcacgttttt
    14281 gtaaacgctg aatggcacgc tcgcaactgt ctaaatcggc aagtgccaat tcggtattga
    14341 tgatttcaat atcatcagct gggtcaattt tccccgcaac gtgaacgatg tcatcatttt
    14401 caaaacaacg cacaacgtgg ccaatcgcat cagtttcacg aatattggct aagaatttat
    14461 tacctaaacc ttcgcctttg cttgcacctg ccactaagcc ggcaatatcc acaaattcca
    14521 ttgtagttgg taacactcgc tcaggtttca caatttccgc caacgcatct aagcgagggt
    14581 caggcattgg taccacaccg gtatttggct caatcgtaca gaacggatag tttgctgcct
    14641 caatacctgc tttagttaat gcattgaaaa gggtcgattt accgacattt ggtaaaccca
    14701 caataccaca tttaaatccc attttttgtt ccttagaatt cccctctccc cttgcgggag
    14761 agggataaat ttgagcttcg ttcagaagcg aaaatgaggg agagggcgaa attgcaataa
    14821 tttcctcaaa tatgaccgct cccctctctc tgaaaaatac ttctgaacga agtatttttc
    14881 ggtctctctc ccacaaggga gagagtttta atttgcttta aaactattta aacggttagt
    14941 tgccttaacc attccgtctt taaataaaac ttctacgcaa cgtgcggctt catcaaccgc
    15001 actattcagt aatgtctgat cgcttggtga aggcttgccc agcacataat tcgccactaa
    15061 gtctttatgc cccgggtgtc caataccaat tcgcacacgg taaaactctt tattattgcc
    15121 aagggcggca ataatatctt tcaagccgtt atgaccaccg tgtccgccgc cttttttcaa
    15181 tttcaccgaa ccgggtggta aatccaattc atcgtgagcg accaaaattt cttccggttt
    15241 aatccgataa aaattggcta acgctcccac tgctttacca cttaaattca taaaagtagt
    15301 cggcactaat aaacggactt cgccttgagc ggtattaatt tttgctactt tgccaaaata
    15361 tttcggctct tcttttaggc taacgttata tattcgggca agttcgttaa tcagccactc
    15421 gcctgcatta tggcgggttt cttcatattt tacccccggg ttcgccagcc cgacaattaa
    15481 tttaatttct gacacaatct tgtctcaatt catttaattt aaaaggcagt attttagaga
    15541 aaaaccgcta ttttctcaag gcaaccatta atcatattgt ctatacggat ttggtacgca
    15601 ttgatacttc gctaacatcg attcagtgaa atcggtgcgt gaagcagcgt tatcattgtt
    15661 taaactaacc acaccaattc gcttgcctct gcaatcccct ttagcaataa aggcaagatt
    15721 ataacccgcc tcacgggtat aacctgtctt actcactaat gcctgatagg attgggaacg
    15781 aaccatactg ttggtgtttt tcatagagaa ataacgacca cctgcataaa cactcactaa
    15841 tggcgaatta gacaattgct gaatttcagg cttacttgcc gcatagcctg ccaatttagc
    15901 aagatcaacc accgttgaaa cattatccga agataagcct gagctatcac caaagtaggt
    15961 ttgagtcata ccgatttcac gggctttgct gttcattcga gccaaaaatt gggctttatt
    16021 caaatgtgtt gcgtgggcaa gggcgtgagc ggcatagtta tcggagcgaa ctagcatcgc
    16081 ttttaacaat tcactacagg caatgttaag atttttcggc aatgggctgg tcgtgccttt
    16141 taagaaatca gcatctgacg gcaaaatttg ggtttggcaa ctcgccccat agcggttttc
    16201 ttctaagaaa acgactgctg tcattaattt tgtgaccgaa gcaatcgggc gaactaaatt
    16261 gaaattctta ccttccaaaa atttcttttg gtttaagtca aacaccacat aagcggaatt
    16321 ttcggttgga atataagcag cggttgcttt aatccgtggt gtgcttttcc tttttgccgg
    16381 ctgggaggaa caggcgctta acaccagagc cgcaatcata acaggaataa attttttcat
    16441 cataatagat tcatcgataa taaagtcaaa gctggttatt ctaccctgtt tccaaaattg
    16501 tgccaaagca agcggtcgat ttttacaaaa tttttgcaaa tctagccgat tttgcaaaat
    16561 ttccgccaaa tttaaccgct tgccctctcg cctcaaatca gcgaaaataa cgcttaattt
    16621 tttggcggac tttccgccct ttttatttta attggttgag aaaaatgtca tacccacaag
    16681 aagttaataa acgtcgcacc tttgcgatta tttcccaccc cgatgcgggt aaaaccacca
    16741 ttaccgaaaa agtattgctc tacggaaatg caatccaaac tgccggctcg gtaaaaggca
    16801 aaggctcggc acaacacgct aaatccgact ggatggagat ggaaaaacaa cgtggaattt
    16861 cgattaccac ctccgtgatg cagttcccgt acaatgattg cttggtaaac ttactcgata
    16921 caccggggca cgaagacttc tcggaagata cttatcgtac tttgactgcg gtagatagct
    16981 gcttaatggt tatcgactcg gcaaaagggg ttgaggaacg caccattaaa ttaatggaag
    17041 tgacccgctt gcgtgatacg ccaatcctaa cctttatgaa caagctagac cgtgatattc
    17101 gtgacccgat ggagctgtta gatgaagtgg aaagcgtgct gaaaatcaaa tgtgcgccga
    17161 tcacttggcc gattggttgc ggaaaattgt ttaaaggggt atatcacctt tacaaggacg
    17221 aaacctactt ataccaaacc ggacaaggtc ataccattca agaagtacga gtggttaaag
    17281 gcttaaataa tccggaactg gatcaagccg ttggcgatga tttagcccag cagttgcgtg
    17341 atgaattaga gctggtacaa ggcgcttcgc acgaattcga tcacaccgaa tttatcaaag
    17401 gcgagctgac accggttttc tttggcactg cactgggtaa cttcggggtc aatcatttcc
    17461 ttgacggttt aaccgaatgg gcaccggcac cacaagcccg tcaatcggac gaacgtgaag
    17521 tttcaccaag cgaggaaaaa ttcagcggtt tcgtatttaa aattcaagcg aatatggatc
    17581 cgaaacaccg agatcgagtt gcttttatgc gtgtcgtttc aggtaaatat gaaaaaggaa
    17641 tgaagcttaa acacgtccgt atcggcaaag atgtggtgat ctccgatgcc ttaaccttta
    17701 tggcgggcga ccgttcacaa gccgaagaag cctacgccgg cgatattatc ggcttgcaca
    17761 atcacggcac gatccaaatt ggtgatactt tcacccaagg cgaaaatttc aaattcaccg
    17821 gtattccgaa cttcgcccct gagctgttcc gccgaatccg cctgcgtgat ccgctcaaac
    17881 agaaacaact gctcaaaggc ttggtacaac tttccgaaga gggtgcggtg caagtattcc
    17941 gtccgttaat caacaacgat ttgatcgtgg gagcggttgg ggtgctacag tttgatgtgg
    18001 tggtttcacg gctgaaaacc gaatacggtg tagaggcaat ttatgaaacc gtaaacgttg
    18061 ccacagcccg ttgggtcgaa ggcaaagatg ccaaaaaatt tgaagagttc aaacgcaaaa
    18121 acgaggcaaa ccttgcccta gacggcggag acaacctcac ctacattgct ccgacaatgg
    18181 tgaacttaaa cctcgcccaa gaacgttacc ctgacgtaca gttctttaaa acgagagagc
    18241 actaaaacca ataggggcga aaaatctttc gcccctacaa ataaagcaca agcggtcaat
    18301 tttttttccg aattttacaa tactaggcat tctgttcctc aaatagccta aatattcccc
    18361 tatcaacaat ccttatcttg agattattct gcctattaat taaccactca aaaataccct
    18421 cgcagaaaac tctacttttt ttatggaaat atgctaaagt tcgccaatat tcatagccct
    18481 gattttttta tggagaaaat aaaatgaaaa aatggttgat tccattaact gtcgcagcgt
    18541 tctgtgtttc tgcttgcgat gataagaaca gcactcaagc aaccgcagaa aaaccgcaag
    18601 tagagaatac tcaatcgcaa aaaaatgcgg aaatattacc gcttgcaacc gtggttgccc
    18661 catgggaaat caatagcctt gacccgaatc aatcaggcgt aattttccag cgtatgaact
    18721 tagcggaaac attggtcgaa gccgatctta acgggaattt aattccggca ttggccactg
    18781 actggcgcag taacgaagag ggaacaatct ggcgttttac cttgcgtgaa ggggtaaaat
    18841 tccataacga tcaagtactt accgcacaag cagtggtaaa tagtttgaat atcgcaaaaa
    18901 gcaaaccggg tgtgttagca aaagccttta tcaaggaaat tcgagcggta gataacaaag
    18961 tggaaattga gctaactaaa gcttttgtgc cgtttcctcc ctttttaaca cactacaccg
    19021 ccttaatttt ggcacctgaa agctataatg aaaaaggcga agtgacccaa attatcggta
    19081 caggtgcgtt taaacccaca aaaattgaag ctccgcagaa attggaagcg gtgcaatttg
    19141 agggctattg gggcaaaaaa ccgcaagttc agcaagccaa ttatttagcc agcagccgta
    19201 gcgaaacccg tatgttaatg gcacaaagcg agccgtcatc actggtattt aatttagata
    19261 ccgccagtgt agcaaggctg aaagccgatc ctaacctgca attagtcagc ggatctattg
    19321 cccgtactat tcagttgaaa atggacgtgg caaagccgtt ttttaacgac ttagcgattc
    19381 gccaagcctt atcgcaagcg attaatcgca aggcgattgc tgagcaggtc ttaaaaattg
    19441 agaacggagc tgccgatcaa attttaccga aagcctttgc cgactggcgt gttgataccg
    19501 cagaacaagt ggtcgatatt gcaaaaatta aacaaaatct gaccgcttcc ggctatacct
    19561 ttgatgaaca aggtcgctta accaaagacg gcaaaccgtt tagctttacc atgcgcacct
    19621 tctcagatcg tcctgaatta ccggtgattg ctactatctt acaagctcaa tggaaacaaa
    19681 ttggcgtgga agtgaatgtt tcggtgggta atttcagcga aattccggcg gggcatcaag
    19741 acggcagttt agaaatggct ctttatgcct taaattatgg caaaacctta gatccatatg
    19801 gcgtaattgc ctccgacttc gccaatggtg gcagtgattg gggggtaatg aattggcaaa
    19861 atactgaggt agacaatgca gtcaaatcgc tcgaaaccga gcgtgacccg caaaaagcga
    19921 aagcattaaa gcaaacggtt agcaaaatta tttacaatga tttgccaatc attccggtgg
    19981 tttattacca acaaaatgtg gtggcacata aagaagtaaa aaatgtgacg ctagatccat
    20041 ttgaaagacg cttctttttg gagttattaa gtaagtagtt taattcaaaa tactcctcgc
    20101 tttagaaaag ggaaggtagg atttttcagt agtaatttgc gctcttaggt gaataaatag
    20161 tgacagaatt atcgtttttt ctcatttttc acctattttc tccgcttatt tcttctgcca
    20221 aatcttccct tcccctcttt gctaaagagg gggatcagaa cgcataaaat catcttttta
    20281 ataaatgaaa taatacaatg cacaaaattc tcttccgccg tcttatccaa attctactgg
    20341 tgatttggtc ggttggcaca ctcacattta tcctcacccg ccaactttcg ggtgatatgg
    20401 cataccgcat tgctgccagc cgctatggtt atgatcaagc agatagtgct gctgctgagg
    20461 ctgtgcgtgc cgagctggga ttagaccaac cttggtggca aagctacttc agctggctaa
    20521 gtgacttggc tcaatttaat ttaggcaaat cattggtgac cggtgatccg gtctggtctg
    20581 aaattcagca ccaattcggg cataccttaa gccttgcgat tgtggcactt atcttagcaa
    20641 tccttatcgg gcctccgctc ggtttgttag ccgcccgtaa gccaaacggc ttttttgacc
    20701 gcttgacact ggtattcagt accctgtttc gctccgtgcc ggcatttatt attgcgattg
    20761 gcttgattac gctgttttcc gctcaactaa aatggctacc tgccggcgga tacggctgtt
    20821 ggcaacattt tatcttgcct gccttaacgc ttgcgattgg cttgagtacg gtttcggtac
    20881 gagtcacccg ttctgcaatg gttcaggtaa aaggctcaga attttaccaa ttttcacaat
    20941 taaaaggctt aagccgttgg catacctttt tacgtcacgg tttgccgaat attgccgtgc
    21001 cggtcattgc ctaccacgca gtacaattag tttatttaat tgaaggcgtt gtggtggtcg
    21061 aaagcctatt tgcttggccc ggcagcggac acgcactggt tcacgccatc attgcccgtg
    21121 atgtgccgat gattcaaggt acaacgctcg tgatgggcgg attgtttgtt gtattaaaca
    21181 ccattgccga tctattaagt gcttggattg acccacgaat taagcgatag gacatcaaga
    21241 tgaaaatttc aatttcacaa tatttaggcg gcactattct ccttgcaatg ttgggatttg
    21301 cctttttaca gccttatttt tacccgatgg atattggctt tcaagattta accaatatcc
    21361 tttccaatcc aaatgaaatc gcttggttcg gcaccgatca tctcgggcgg gatatgcttg
    21421 cccgtcttgc ctctgctatt cgtctttcgt ttggcttatc actgtttagc gtgatttgtg
    21481 ccttaattgc aggcttaatt accggcatta tggcaggctt tttcggtggc tggattgacc
    21541 gcattttcag ctttatttgt gatctagtga tggcactccc cggcttactt ttagtattgc
    21601 tctttgccgc cctttctccc ggctcatttt ggacgctcta tttagggatt tcattggtaa
    21661 tgtgggtgga atttttccgt gtgatccgct ctatcagttc aacaatggca aacagctcgg
    21721 aaattcaagc ctcacgccta atgggaatgg gctggtttta tagcttcaaa cgccacttat
    21781 tgccccgttt attaccgatg attgccactt tatcggcatt ctcgctcggc aatgcgattt
    21841 tagctctcgc cacacttggc tttgtcagcg tagggcttcg cccgcctaca gcagaattag
    21901 gcttgatgat gaccgaacta ttcccttact actacgaagc accgctgata tttttgcaac
    21961 cagtggttgc cgttttctta atggtactca gcttacaatt attgtctaga aaaattaaat
    22021 aatgcgtagg ggcaggtcct gtgcctgccc aaaattctta ttatatttat gggcgggcac
    22081 agggcccgcc cctacagtaa taaaatgaag atgaaattac tcgatatcca aaacctatct
    22141 gttgaaaccg aaaacagatt acagctggtc gaaccgatta gcctacaatt aaaggcagga
    22201 aaaaatatta ctatcttagg cgaaaccggc tcaggcaaaa gcctgctgat tcaagcgatt
    22261 atgggggcgt tacccgacgg cttaatcgca agcggtgaaa tttcggtaaa aaattgcaaa
    22321 atttccaccg aatccgaccg cttgcaaaca ttgtggggta aaaccttggt aatgctgcca
    22381 caagagcctc gccgctcgct taatccgatt atggcaatcg gcaagcagtt gtgggaaagt
    22441 tttcactatg tggcaggcaa accatcatct caagccaaaa cggaaagcaa agcctattta
    22501 gccaaacttg ggctgggcga atcggttgaa aactacccac accagctttc cggtggaatg
    22561 gctcaacgtg cctcttttgc gattgcaact gccgccggtg gcaaaatttt acttgccgat
    22621 gagccgacca aagggcttga cccaatcagt aaaggcaaag tgattgaact gttaaaatcc
    22681 gcctaccaaa acggtggagg cttgctcacc attactcacg atattgaagt tgccgagcaa
    22741 ctcggtggca ctattttagt gatgaaaaaa ggcgaactgt tggagcaagg tgaggcaaaa
    22801 gcgttgttaa ataacccaca acacccttac accaaagcac ttatcagtgc cgatccaaaa
    22861 cattggcaac cattgcaaga tctgcaaaat ttaagccaaa aacaaccgct tgtttcggtt
    22921 aaaaatttga gtattgcccg tggtaagaaa acgctgttta gcaacctttc ctttgactta
    22981 cacaaaggcg aagtgcttgg gattgtcggg catagtggga ttggcaaaag ctcgcttgcc
    23041 gatgtacttt gtggtttgct taagccgaaa tcgggcgagg ttgtgtggca cagccacagc
    23101 cataaaaaac accaagtgtt gaagctttac caagacccac cggaagcctt tgctccgaat
    23161 gtcagtctaa aaaccttgct tgatgatgtg attaacaagc accaattaga tcgactgccg
    23221 attccaagtt tactgcaaca actttcgctc aacccagaaa ttttgcaacg caatgcggaa
    23281 aatgtctccg gcggcgagct acaacgagta gcaattttgc gagccttgct cttagaaccg
    23341 gtactactgt ttgccgatga agtcacttct cggcttgacc caatcacaca acaagaaacg
    23401 atagaattgc tgatcaacca gtgccgagcg agaaattgca gtctgatttt ggtcagtcac
    23461 gatccttatc taattgagaa atcgtgcgat aaagtgatta atttggaaga gtatgtgggc
    23521 taacaagcgg ttcaatttct gagatttttt gcggctctaa aaacaaaaat gcgaagacat
    23581 cagttcttcg catttttctt acttattcct tattgcccag ccagcttctg ccgtacaatt
    23641 tcaaacagac agaccccggt tgcaactgag acattcaaag aagatactga gcccgccatt
    23701 ggaatgctga taagctggtc gcaatgctca cgggtaaggc gacgcattcc gtcgccttct
    23761 gcccccataa ctaacgcaat cgagccggtt aattttgctt cataaatgcc cgattccgcc
    23821 tcacctgccg tgccgacaat ccagacatta tgccgttctt gtaaatctcg cattgtgcga
    23881 gccagattgg taactcgaat cagtggcatc acttccgctg ccccacaggc gactttgcga
    23941 gcggttggtg taagctgagc agatttatct ttcggcacaa taaccgcatc aacccctgcc
    24001 gcatcagcgg tacgcaaaca agcccctagg ttgtgagggt cggtcacgcc atctaaaatc
    24061 aacaagagtg gatttttctt ttggcttaag atcgcctcta aatcgtgttc attcagctct
    24121 ttttgtggca cgactctggc aataatccct tgatgcactt caccttgcga tttgttatct
    24181 aaggtttggc ggttcacttg ttgaattgcc acccctaaac gttgtaattc atttaatagt
    24241 ggcattaagc gtttatcttc acgccctttt agcaccagca cttcaattaa acgctcaggt
    24301 gcattatcca aaaaggcttt cactgcgtga atgccataaa tttgttcgct catattttat
    24361 ccttaataat tgggcgaacc catcagctcg cccatataaa tacctaacta aagcaacatc
    24421 ttcacaattt tatccaactt aaccctgccg aattttttca gcaatttacg cacattttca
    24481 gggtaatcgg ttaaaacttc caaatcactg tagtgagtta aaatctgcgt atgttggcaa
    24541 atttggttta actccgtttc tgctttttca cttaattgat gttgaggatc ggaaattaaa
    24601 attgcatttt cggcatctaa ccgccacgct cttgggttaa ggttattgcc ggtcagcaaa
    24661 atatagcggt tatccaccca aacccctttt aagtggtagc tgttttcgcc atctttccat
    24721 aagcgaatgg ttaactgttt attttgtaca taaccgtcca accgttttgc aaaggctcgt
    24781 aagttctttt catacaaata aggcagagcc gatgccatcg tgaatttttc gtccggcttg
    24841 gtgtagaaat cattagcggt tttatccccc acaataattt caaccgattt gccgtttgcc
    24901 aatagccatt caatacgggt tctcagagaa tgcggaaaat taaaatacgg agtgcaaatc
    24961 gttaatttct gctcagtttg ataaaacaac gcttcaatca ctttatttaa ctgattctta
    25021 cgtcgcccta agcctactaa aggagaaacc gctaagcgac cattttcagc aacaccgtta
    25081 aataaatacg cttcccgact taattgtttg cggaaatgct taatttgcgc acgaatttca
    25141 gccgttttcg ggcgattttc cgtatctaaa cgttttaccg cttcattggt taaaagatgc
    25201 tgttgaataa acgtcaccca gctatcggca agaactttat tttcaatgac gtggtaacgg
    25261 tcataacggt aacgttcaaa ttgatgcaaa tacacattat taatgcttgc tccgctatac
    25321 aaaatcgtat cgtcaaatac gaagcctttc aaatgcaata caccaaacac ttctcgtccg
    25381 ttaatcgaca caccgtaaaa ggcaatctct tgccctgccg gtaaaccttg ttcgtattta
    25441 acttttgcat accagtcggc gttagataaa ttctctgcct caccaatacg cccacgttgg
    25501 gctcggtgcc aatcaaccaa aatgttaatt tccagttcag gattatttcg cttcgcttga
    25561 tataaagcag ctaatacttc ctgcccggct tcatcttttt caaagtaaag agcggttata
    25621 taaatacgct tttttgccgt ttgaatcagc tgtaaaattt gttgtttaaa cgctttgctc
    25681 gaggcgagaa actgaacctg ctctgccact tgtggtaagt atggcaggct tttcaatagg
    25741 tattcagctt tacgttgttt gttcaaaatt aacatcattg attccaaaat tttcaatcgt
    25801 tgcctgctat tttacactga aatcgccaat aaagcgattt tcgcttaacg gcattgatag
    25861 ctggtcaaac gattaggatc ttctccggtt tgataaattg cgactttcgg ataagcacaa
    25921 agcgggatct gcttggcttt attagggtaa agctcaccgg ctttggcggc aatactttcc
    25981 ggctccagtc ctttctcaac ccaattttca agggcagtga gcatatcaaa atcattcacc
    26041 gttgcccctc gcccacaatg gttcattgcc gacacgaaaa acaattttgc aaaatgttgc
    26101 ggattttcga ccgcttgttg taggctttga taccaatttt tgagatcaac tgccgaaaaa
    26161 atcgggtctg aaacgccttg ataaatcagc attttgccac cgttctgctt aaaagcggat
    26221 aaatcaactt gatctgtatc gtgaatgctc cctaccgctt tcacttgttc gctggcttga
    26281 tccaaatcta cgctgagtgg atcacggctc ggcactctgg gggctaaata gtaatgggtc
    26341 aaacttttca atcccatttt aaaactaatg ctgttgggct gagcggtttg gctatcacct
    26401 aatttccaat cccgccagcc tttagcgtta atgccggaat catacgccca agggctataa
    26461 atcagctcgc ctcgactgtt tttcgccccc tcgaaaatcg ttttgattaa agcgactttt
    26521 ttctgcccca gttgctttgc caccatttcc ggctggaaat cacactgttc ccacgcatta
    26581 attaaaccgt ctgctaagcc gtctaactta tcgcaacgtg ttaatacgcc attggcaatc
    26641 acatctaaat cttgttgagt gagggcttgg gataaaattt tgtcgccatc accattcttc
    26701 ggagcaactg ccaacaaggc tcgattatcc catacttccg ccaagactga acgggaaacc
    26761 cgaaagccgg ggctgcctgc aatcacaccg tcaaattcct gtggataacg cattgccgcc
    26821 atcatcgcct cacgaccacc attcgagcag cccataatgt aattgtgctt gctcggtttt
    26881 cggtaaaatt cggcaatcaa ctgcttggca accgtggtca ctttgccgat agaggcatag
    26941 gcataatttg agcgagcttc gggatctgcg gtaaacacta aatctcgagt ggcgtgacca
    27001 ctgtcgttac tcactactgc atagcctctg tttaagccat ttgcaccact cgccccgtgc
    27061 ggatagagct taccaatggc agggaaaatc accccaccta caccgcccgc cccctgaaat
    27121 aagaattttt cattccattg ttcgggtaaa cgtagctcaa attgaatacc gtaaggaaca
    27181 ttatccacct ccacccgttt tccaatttca ccttgtacta aacagtaagc cggcaactca
    27241 gcagatttct caacccattc agctttataa atagccgttt tttccggcga gttagatttt
    27301 aacgcgacac attgttcagc catagaatta gccaaagcag tgtgagaaaa aacggagaga
    27361 aaaataaaaa gaaaagcgat tattttcatc atttacatca atacaagcgg ttatttttcc
    27421 gaaaattttt gcaaatttta aactgagtgt ctatctatcg ctggaaaagc taaggcagag
    27481 tttcctctgc cttagaaaga tgaattgaat tacattttaa gcaaacaagc gtttttcgcc
    27541 ttcgccggct acgttttcca cacaacgtcc gcaaacttct tcgtggccag agtgcgagcc
    27601 gatgtcggta gcataatgcc agcaacgagg gcatttctca ccatctgcat tttccacttt
    27661 tactgcgagt ccggctaatt cgccttccgc aatatctgct tccgccagtg gtttaacaat
    27721 cgcttgtgag gtaatcagca caaagcgtaa ttcattgcca agttgttcta ataacgggcg
    27781 aatttcatca ttggcgaata ccgtcacctt ggcttctaag ccagcaccaa ttactttgtc
    27841 tttacgggct tgttccagca cacggttgac ttccgcacgc acttttaaga tttgttgcca
    27901 gtaagcatca tctaatttat cttgctcggt taaaccgaat aatccggtgt agaattcttc
    27961 ggtaaagaca aattcacttc tgccttccac ctgcggtaag tagccccaaa tttcatcggc
    28021 tgtgaaggat aaaatcggtg caatccaacg tactaacgct tctgcaatat gccataacgc
    28081 tgtttggcag ctacggcggg caaggctgtc ggctttggtg gtgtattggc ggtctttgat
    28141 aatatctaag tagaaagacc ccatttcaat cgagcagaaa cgcattaaac gctgtactac
    28201 cgtgtggaat tggtagttat cataggcttc tttgatgtcg ttttgagcct ctaaagcaca
    28261 agccaccgcc cagcggtcta aggcgatcat ctcttgcggt tgaactaaat cactttttgg
    28321 atcgaaaccg ttcaagttcg ccaataagaa acgggctgtg ttacgaatac ggcggtatga
    28381 ttccccggca ctgtttaaaa tattgtgcga aacggtaatc tcaccggtat agtcggtgga
    28441 ggcaacccat aagcgtaaaa tatctgcacc gtttttgttc cacacttcac ttggcacaat
    28501 tacgttgcca agcgatttgg acatttttct gcctttctca tctacggtaa aaccgtgagt
    28561 tagcacttgt ttgtatggtg ctttgccgtt agtagcagtg gaaagcatta aagatgacat
    28621 aaaccaacca cgatgttggt ctgaaccttc taaatacata tccgctgaat tgccgttaaa
    28681 ttcagggcgt tgttctacaa cagaggcata ggtcgatcct gaatcaaacc acacgtctaa
    28741 ggtatccggc actttgcggt aatctttggc ttcttcaccg agcaattccg caggatctaa
    28801 atcccaccac gcttgaatgc ctttttcttc tacacgtttc gccacttctt caattaattc
    28861 taaagtgcga ggatgtaatt cttcggtttc attatgcaca aacatcgcca ttggcacgcc
    28921 ccaagtacgt tggcgtgaaa tacaccagtc tgggcggttt gccaccatcg tatcaatacg
    28981 agcttcaccc cagcttggga tccaacgcac cgatttgatt tctcctagtg cttgctggcg
    29041 taagccttgg gtttccattc cgataaacca ttgtggggta gcacggaaga taatcggggt
    29101 tttgtgtctc caacagtgcg gataactatg cttgatgcgg gataatttta ataatgctcc
    29161 cacttcttgt aatttttcga ctactttatc gttggattca aacacaccta agccggcaaa
    29221 gaacggggtg tctgctttga atttaccgtc attggaaatt agccctgcca tttcaatgtt
    29281 atatttgcga gcgaccacga agtcgtccgc accgtgatcc ggtgcagtat gcactaaccc
    29341 tgtaccaccg tctgtggtaa cgtggtcgcc aaaaataaat ggcacgctgt aatcatagaa
    29401 tgggtggtta aagcgtaata attctaagtt ttcgcctttg gtggttgcta aaatctcagc
    29461 agattcaacg cctgctgctt ttgcaacatc ttcgactaaa tcgaccgcta gtaacacacg
    29521 ctcatcacct aattggacta aggcatagtc taattcaggg ttgatcgcaa tcgcacggtt
    29581 agacggaata gtccaagggg tagttgtcca aataaccgca gataatgccc ctctaccttt
    29641 accctgtgcg ttgaattttt cttctaccgc atttacgtcc acagcattaa aacgcacata
    29701 aatagaggga gaaactttat cttcatattc cacttcagct tccgctaacg aagatccaca
    29761 atctaagcac caatgtaccg gttttgaacc tttgtataaa tgcccgttcg caatcacttt
    29821 gccgagcgtg cgaataatat tcgcctcagt gttaaaattc attgttaggt atgggttatc
    29881 ccaatcacct aaaataccca tacggataaa atcggctttc tgaccttcta cttgttcttt
    29941 cgcatagtca cggcacgctt ggcgaaactc ggcagccgaa attttctcat ttggtttacc
    30001 aacaatccct tctactttca gctcaatcgg caaaccgtga cagtcccagc ccggcacata
    30061 aggggtatcg aaacctaatg cggttttcga tttcaaaatc atatctttta aaattttatt
    30121 cactgcgtga cctaagtgaa tattaccgtt agcatacgga ggaccatcat gcaaaataaa
    30181 ggttttcttg cctttcgagg cagtacgaat tttttggtat aaatttttgt cataccaatt
    30241 ttttaacata tcgggttcac gtttcgcgag atccccacgc ataggaaagc ctgtttcagg
    30301 caagtttaag gtgtttttgt agtcaatagt cattttttta tttctcgtta aaattatctg
    30361 ttttatgcct agcacggcat agccgtacta ggttaagcat agctcagccc ctctggggct
    30421 ggaaactaga aaggctcacc ttgcaaattc cttaaaaaat tcaaccgctt gcaagcgatc
    30481 tttctcaatc tgggctttta gtgcttcaaa gtcgggaaat tttatttcat tgcgtaattt
    30541 ttttaaaaag atcacttcaa tcgcctcacc gtaaatagag gcattgaaat caaaaatatg
    30601 cacttctaat aacggcttag taccgttaat ggtcgggcgg ttgccgacat tggcgattcc
    30661 gttaaaactg ccctgtttgg tcttcacttt taccgcaaat accccttgaa tcggcgtaac
    30721 caaacgattg agcataatat ttgcggtagg aaaaccaatg gttctgccga gtttattacc
    30781 gtgtgcaacg cgtcccagaa tcgaataagg cttgccgagt aatttctctg ccaaatctaa
    30841 ctcattgttt tgtaaggctt ctcgcactaa tgagctgcta attctttctt cgtttaagct
    30901 atgggtatgg ctttcttcca ccgcaaagcg atattttaag ccggcattgc gtaaagtttc
    30961 aaaactgccc gcccgttttg ccccaaagcg aaaatcatcg cctacactta aatagcgtac
    31021 tttcagtctt ttagcgagca aatcgctgat gaaatcgtct gccgaaattt gagcaaattt
    31081 ttcactaaaa cgcacgcata gcacaaaatc cacaccggct tcttccaaat attttagctt
    31141 atctcgcagg cgcattaagc gggcgggcgg gttacaagcg gttgaatttc cacttttttt
    31201 tgcaaaaaac tctcgaggtt gtggctcaaa taacatcaca actgagggca atcctaaatc
    31261 taaagctcta tcacaaagac gagccaaaat attttgatgc ccaagatgca caccatcgaa
    31321 attcccgata gagagaacac agccttgatt taaagcgatg tgtttgtcta agttgtggaa
    31381 acctcgaata agttgcataa aagataaacc at
//
LOCUS       DANLYI010000006        30727 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.006, whole genome shotgun sequence.
ACCESSION   DANLYI010000006 DANLYI010000000
VERSION     DANLYI010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 30727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30727
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.006"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            complement(4..456)
                     /gene="ssb"
                     /locus_tag="PXD81_000206"
     CDS             complement(4..456)
                     /gene="ssb"
                     /locus_tag="PXD81_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDL5536279.1"
                     /translation="MAGINKVIIVGNLGNDPEMRTMPNGEAVANISVATSESWTDKNT
                     GERREVTEWHRIVFYRRYAEICGQYLRKGSKIYIEGKLRTRKWQDQNGQERYTTEIQG
                     ELLQMLDSRNATNSVDGQQSNYQAMSQSPKATHPQDLPMDNFDDDIPF"
     gene            complement(812..1297)
                     /locus_tag="PXD81_000207"
     CDS             complement(812..1297)
                     /locus_tag="PXD81_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012340855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3158 family protein"
                     /protein_id="HDL5536280.1"
                     /translation="MNLISDEIYRQLVKDSGLNTSLKQLFSHFDTGSDYELLQEQFTQ
                     ARAYFMAAQDSMVQSVRQDLSPLAVYMIKDKASSSGGTFLRWRSMQNARTGGTVWQPI
                     VDDKSVPVEVRKKVVAVEKDRILINMQISVFNHILRQLADCAEKLKEVDNAVAKSDLN
                     S"
     gene            complement(1306..2064)
                     /locus_tag="PXD81_000208"
     CDS             complement(1306..2064)
                     /locus_tag="PXD81_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012340856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03761 family integrating conjugative element
                     protein"
                     /protein_id="HDL5536281.1"
                     /translation="MTTQLDPQLGPLRSEITFTLHTQYAHKLWVGRPMIRNEEGKVTQ
                     SSIISVPNCFAMLTQIQRAASEDDPYADDYLIQFEESVINYRKEIQKLTNDIVTLYAK
                     MLPEGISIERCANISPISYPIYVNSQLGYQLLYLLGDFDSLCRSVMTAAHIAIINRAE
                     AQDWIEAGARLIRKCFGIVERYKSSGITRRDYQEKNARYQAAIKRMGYTLSDAVLTGE
                     HRAEFAPFIKQNTTVEEEQSVETHITAQTNESQK"
     gene            complement(2243..3433)
                     /locus_tag="PXD81_000209"
     CDS             complement(2243..3433)
                     /locus_tag="PXD81_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5536282.1"
                     /translation="MFSSTNKPTEHGLLFFGNQHETVPTRLLQDPCLTPRAKFAWQLI
                     KCNVQAFQGGLFPSYETLSKMLSDRPYAEAKLSEKVVTQTLYLLRLTRWLTLCETVRD
                     ERGYVQGNVYILHDEPVPILDAIQLNSDYLSFLDKCTKHKDSLINGVANHIVESLITD
                     KTKWHYVSHLDCLQSRYQIYQQQQLVKDKQPSQTEQELSKIQQNILTSTMEVRDFDEE
                     VRKKSSKNQTSNKGVRAKNEDKSLILDLLPLGKSDVQYRTLYINKYCTGEPSEITWPQ
                     EIPLTSLEQKLTAQAMKGLDLGLCQAILLEVQQRIIQKQDVKKPKGYLYTLIQRAKQG
                     EFKPYYFELGKNLPSQNQANNNSAHSIQKLTEQPTEKPVLTSADREQMASRMQKFRAE
                     LLLQ"
     gene            complement(3592..4149)
                     /locus_tag="PXD81_000210"
     CDS             complement(3592..4149)
                     /locus_tag="PXD81_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2857 domain-containing protein"
                     /protein_id="HDL5536283.1"
                     /translation="MIKPITVNEALIGNALLNIKEGNIHPCLKMGFSEEQLKAINNLS
                     IDEIMDISNSVVSFAKVEINHETFWKLLAIAQANTQQRQIIDRALLLGASIEMLHQYF
                     GLSTSEVSARRQLLGIEEKMGRKAAASDEESTHIWEIWQRYKQRMEILDSQEGLELLC
                     LIAEEGNMNLTVVWKLVSEWYSKHK"
     gene            complement(4142..5788)
                     /locus_tag="PXD81_000211"
     CDS             complement(4142..5788)
                     /locus_tag="PXD81_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012340859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HDL5536284.1"
                     /translation="MTKDEKRLAAIAKGLNVSPISNTAPSYQTATPTHYSSDVEYITV
                     TLDKLRPYEHNPRKTRNPNFEMIKESIRRRGLDHKPNITRRPGEDFYIIADGGNTRIQ
                     ALKELFTETKDPKFWSISCQYKPWQGDTADSVEAELNLLIGHLIENDTRADLSFIEKA
                     LGILQAKEYYEKKLGKSLSSRELSSELENDGYIISHALILKMERCVNYLYPHIPEVLF
                     SGLGHTQIDKLLAIRNNAEEVWAAHQFDTDVTFESLWSENLSKFNEATPFQAKEFQSE
                     LITAMVEALEGKVSFETLYLDIDLDEQKFKKIAAKQQEIDNLVEKTVDQINTRQEQQT
                     AKSEPLIKNENKAVTPQKAQHIIDEVIDDNESDDEQNNAITDLSQLGSSLLDNQDNQE
                     DYLSQIAHNFGLTPGMSIQEQREQRAEANGLTFACEGRQPVEDIWLIHPARQYRSEAY
                     SLALDIAESMGIDHLVEHIVKQPVDYTYAMKPADTELSPSALTLYSFLSMLQTAEHKD
                     ENSCVLDSKFLFELDDLTLVKIFRLIRVSRHIRQQGGEND"
     gene            complement(5797..7158)
                     /gene="dnaB"
                     /locus_tag="PXD81_000212"
     CDS             complement(5797..7158)
                     /gene="dnaB"
                     /locus_tag="PXD81_000212"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HDL5536285.1"
                     /translation="MSSLNKSLVQPHSIEAEFSVIGGLLLDNELFDEVSSKINEGDFY
                     NFTHRLLFKHISELIEAGKPADILTLDQYLTQKKVIEDLGGFAYLAEIVKNTPSTANI
                     HAYTDIVLLYSKQRQLLKLGQFIVDQTQSAKTPDKFEAVVDDIEKKITEFSLSDNTKS
                     AADLSDIFANMLSRMELSAKNGDPVTGTPTGIQGIDEATTGGQPGDLIVIGARPSMGK
                     TAFSQTIAYHTLKKFESAPVFYHSMEMPADQILQRFLAMRARVGLQNIRQADSLDDEE
                     WSKLTSAMGYILEHWKNRLVIDDEGSLTPQKLRTKVRQNARKYGKPAAIFIDYIQLMQ
                     TSQRYENRNLEIANISRSLKLLAKEMDCPVYALSQLNRSVEQRANKRPINSDLRESGS
                     LEQDADVILFIYRDEVYDADSPFKGTAEIIIGKQRNGPLTSVMTNFRSEFALFENRTN
                     TTH"
     gene            complement(7170..7994)
                     /locus_tag="PXD81_000213"
     CDS             complement(7170..7994)
                     /locus_tag="PXD81_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012340861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HDL5536286.1"
                     /translation="MKPLQNGTNKPFTITIASTKGGSAKSTNAANIGAFCAEHGLRTL
                     LIDTDTQPTLSSYYELEYQAPGGTYEFLHFKDVDPTHIISRTTIPNLDLIQSNDPSNK
                     ISPMLRDSPDGALRFSLLLQKIEGYDVIIIDTRGTRDITVDMSVLAADVLFCPILPHI
                     LSAKEFIRGTIGMYQDLETFTAFGFTLPPLKAMINCVDHTKDVKLVSDYLHTLFQNEF
                     DENKTLLEFSVPDRVAYREAATFSKPVYRQSQAEYETISQLCALLMPQFANSHFSA"
     gene            complement(8092..8177)
                     /locus_tag="PXD81_000214"
     tRNA            complement(8092..8177)
                     /locus_tag="PXD81_000214"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8141..8143),aa:Leu,seq:caa)
     gene            complement(8489..9949)
                     /gene="xseA"
                     /locus_tag="PXD81_000215"
     CDS             complement(8489..9949)
                     /gene="xseA"
                     /locus_tag="PXD81_000215"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015587093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HDL5536287.1"
                     /translation="MSNILTVTQLNYSVRHLLEMEMSHIWLTGEISNFSQPVSGHWYL
                     TLKDDKAQVRCAMFKMKNVRVNFRPQNGMQVLVRASVTLYEPRGDYQLIIESMQPAGE
                     GLLQQQFEQLKAKLSAQGLFAQEHKKPIPTFVKTVGIITSSSGAALQDILHILQRRDP
                     SLSVVIYPALVQGKEATQDIVNMIELANRRNECDVLIVGRGGGSLEDLWCFNEESVAY
                     AIYHSQIPIISAVGHETDVTIADFVADLRAPTPSAAAELVSRDQNELKRQLQHQFDKV
                     NLAFDRVWNHKFNRFQQLPLRLNAQHPSKQIQSQQQRFDYMEYRLEQAIYALVAAKKQ
                     VGKELNVRLIHQHPQKQIERQSQMLKQLELKLHWQMEQQLSKKQQKWKNITQRFERNP
                     LPYQLYQQQTMVAKMSQRLAYSIEKIMLKEQRHFQELCTKLDGLSPLKILTRGYSITQ
                     TKDGKTLTSTENIERGEAIITQLKTGKIISQVVDFL"
     gene            complement(10100..10176)
                     /locus_tag="PXD81_000216"
     tRNA            complement(10100..10176)
                     /locus_tag="PXD81_000216"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10140..10142),aa:Met,seq:cat)
     gene            10386..11492
                     /gene="mltA"
                     /locus_tag="PXD81_000217"
     CDS             10386..11492
                     /gene="mltA"
                     /locus_tag="PXD81_000217"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005597277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HDL5536288.1"
                     /translation="MNWKAYKKAIAAASVVAIATVLVACSSSGQKGKTTSYDAEKAKF
                     GAKYNGRHFMPYGTHFQAGRLGLNAAPVNQLDFLQQLSNVRAYSSLADSYAIAYGKIS
                     RWISSGGNINDLAKYGISTRLMRGEDGYQNVLMTGYYSPVIKARRTPQGQYQHPIYAM
                     PSSKRFSRAEIYAGALQGQGLELAYSNSMLDNFLLGVQGSGYVDFGDGRLNYFAYAGQ
                     NGFKYASVGRLLVEDGEIPKEKMSVQAIREWGQRNPSRVQGLLERNPSYVFFKNDPTG
                     QVKGSAGVPLVPLASVASDRSIVPSGSVLLVEMPLIDSKGNWTGKHEMRLMVALDVGG
                     AVKGHHFDLYQGIGDKAGHIAGLMKHYGRVWVLN"
     gene            11505..11954
                     /locus_tag="PXD81_000218"
     CDS             11505..11954
                     /locus_tag="PXD81_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5536289.1"
                     /translation="MLKKLSAIFLLLPSAVLANNLQNITASTEPKYKISEIDVRILIR
                     QLNNIEQCIYPELAKPGYQQIYANWNLAENLTMQYFEYQLLKELLGEENQKLMQNDTL
                     STEYFHLLHSRLNHQKANVDPEKCDTFKPRYKEIYKSMENALTKKNQ"
     gene            complement(11988..13505)
                     /gene="purF"
                     /locus_tag="PXD81_000219"
     CDS             complement(11988..13505)
                     /gene="purF"
                     /locus_tag="PXD81_000219"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005647425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HDL5536290.1"
                     /translation="MCGIVGIIGNSPVNQAIYDGLTLLQHRGQDAAGIVTIDDENRFR
                     LRKANGLVSDVFQQEHMVRLQGNVGIGHVRYPTAGSSSVSEAQPFYVNSPFGITLVHN
                     GNLTNNAELKARLYNEARRHVNTNSDSESLLNIFAYFLDLYSTQHLSPDNIFETVRKT
                     NDSIRGAYACIAMIIGHGMVAFRDPFGIRPLVLGKREIEGKTEYMFASESVALDVVGF
                     EFVRDVLPGEAIYVTFDGQLHSQICADNPKLNPCIFEYVYFARPDSVIDGVSVYSARV
                     HMGELLGEKIKREWGRIIDDIDVVIPIPETSNDIAVRIANMLYKPYRQGFVKNRYVAR
                     TFIMPGQAQRKSSVRRKLNAIASEFKGKSVLLVDDSIVRGTTSEQIVEMARAAGAKRV
                     YFASAAPEIRYPNVYGIDMPTCEELVAYDRSVEEVAQMIGVDKLIFQDLEALYKSIQL
                     ENPTIHRFDDSVFTGEYITGDVDKCYLDSIARSRNDKAKAEAAKQATNLEIHNER"
     gene            complement(13520..14023)
                     /locus_tag="PXD81_000220"
     CDS             complement(13520..14023)
                     /locus_tag="PXD81_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HDL5536291.1"
                     /translation="MVDLIVIGIIAFSVLISLWRGFTNEALSLLGWVIAFFVASSFYP
                     YLSGYLTQINSVYLQNSEFLRNGIAAAILFILTLIVCGVINGLLGKVIDLSGMSITDR
                     ILGGAFGALRGILIVSAILFFLDTFTQASQTELWKSSLLIPHFDFIVKWFFEQLQANS
                     SFLNTTK"
     gene            complement(14169..14630)
                     /gene="rnhA"
                     /locus_tag="PXD81_000221"
     CDS             complement(14169..14630)
                     /gene="rnhA"
                     /locus_tag="PXD81_000221"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HDL5536292.1"
                     /translation="MKLVEIFTDGSCLGNPGKGGIGILLRYNGYEKTVSKGYFQTTNN
                     RMELRAVIEALAMLKEPCKVQLNSDSQYMKNGIQKWIFNWKKNDWKTSDKKPVKNKDL
                     WVALDQEIQRHQIEWSWVKGHSGHRENEICDELAKQGANNPTLDDVGYIAD"
     gene            complement(14723..16054)
                     /gene="argG"
                     /locus_tag="PXD81_000222"
     CDS             complement(14723..16054)
                     /gene="argG"
                     /locus_tag="PXD81_000222"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005722481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HDL5536293.1"
                     /translation="MSATILEALPLGQKVGIAFSGGLDTSAALLWMRKKGAVPYAYTA
                     NLGQPDEDDYNAIPKKAMEYGAENARLIDCRQQLAHEGIAAIQCGAFHISTGGIPYFN
                     TTPLGRAVTGTMLVAAMKEDDVNIWGDGSTFKGNDIERFYRYGLLTNPKLKIYKPWLD
                     QLFIDELGGRLEMSKFLIENGFDYKMSVEKAYSTDSNMLGATHEAKDLEELSTGMKIV
                     KPIMGVAFWDESVEIKAETVSVTFEEGVPVALNGKRIDDAVELIMEANRIGGRHGLGM
                     SDQIENRIIEAKSRGIYEAPGMALLHIAYERLVTGIHNEDTIEQYRINGLRLGRLLYQ
                     GRWFDPQALMLRETAQRWVAKAITGTVTLELRRGNDFTILNTESPNLTYEAERLSMEK
                     VEDAPFTPADRIGQLTMRNLDIVDTRGKLAVYTDAGLLAANNSVIPQLGKK"
     gene            16238..17278
                     /locus_tag="PXD81_000223"
     CDS             16238..17278
                     /locus_tag="PXD81_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HDL5536294.1"
                     /translation="MKKLLVIRNDKIGDFMVCFPAFAMLKQSMPNVEITALVPSYTAP
                     LAELCPSIDKIIIDTANKKDKNEFNRILQAVKNEQFDAVICFVSDWHNAKLTWQSGIK
                     YRLAPATKLFQFLYNHRLTQRRSQSAKAESEYNLDLARAFLSKHNIPIVEPHTPYLSV
                     EQQTVQAQKAKLCEQLNITTVKKWVFVHSSTGGSANNLSIEQYAELIQGILAEFDCYV
                     VLTAGKGESEKAQQLAQQINHNNVVVYDKNEGLQDFTHSLACADLFIAGSTGPLHISG
                     ALNIPTIGFYPSRLSAIPRRWKPINDANKHIAFCPPSDKASQMNLTLISISEALKTIV
                     PFIRRMWNTPSL"
     gene            complement(17343..18101)
                     /locus_tag="PXD81_000224"
     CDS             complement(17343..18101)
                     /locus_tag="PXD81_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDL5536295.1"
                     /translation="MPTLSVAMIVKNEAQDLRACLDTVKDWVDEIVILDSGSTDETPQ
                     IAAEYNARFYTNTDWQGFGKQRQLAQQYVTSDYVLWLDADERVTAELKQSILQAVKND
                     EKNTVYKISRLSEVFGKQIRHSGWYPDCVVRLYRTEFAKYGDELVHEKVHYPKEAKIQ
                     KLQGDLLHFTYKDIHHYLVKSASYAKAWAIQRANAGKTASLFDGVTHAIGCFVKMYLL
                     KAGFLDGKQGFLLAVLSAHSTFVKYADLWNRTRK"
     gene            18228..18542
                     /gene="yhbY"
                     /locus_tag="PXD81_000225"
     CDS             18228..18542
                     /gene="yhbY"
                     /locus_tag="PXD81_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010786380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HDL5536296.1"
                     /translation="MSITLTTKQKQFLKGLAHHLSPVVMLGGNGLTEGVLAEIDNALD
                     HHELIKVKVAGADRETKQLIIDAILRETKAAEVQTIGHILVLYRQSEEKKISLPKATK
                     AI"
     gene            18603..19325
                     /locus_tag="PXD81_000226"
     CDS             18603..19325
                     /locus_tag="PXD81_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HDL5536297.1"
                     /translation="MFKKFSLYTLAMLLIPVVTWLANWHWSLAIQYAEFDFDYFLYLI
                     TETGSVPYAIITCVVFALLLARVVRKRYSLIFVITVCVASMVATQAIKTAMKSTFQEP
                     RPFVTALFQDKTDDFYQLTREQRGLAVMEKIGANQNFVTAHQAKEVGYSFPSGHTIFA
                     VSWLLLVVGFCRNMRGQAVVLAHFFAISWAALMLISRLRLGMHYPIDLFTSSLIAFLI
                     NLALFVWFVPWLESKKWFAKKP"
     gene            19394..19555
                     /locus_tag="PXD81_000227"
     CDS             19394..19555
                     /locus_tag="PXD81_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5536298.1"
                     /translation="MEMSIEKIAELLSDKEKDDYPFSIEERQFAAQASADTWEAILNN
                     TIQPFDFKK"
     gene            19563..19925
                     /locus_tag="PXD81_000228"
     CDS             19563..19925
                     /locus_tag="PXD81_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5536299.1"
                     /translation="MYQVFIAEPKDPNLEKGLLKHFIKSYLTQEELDFLHKILTNLPN
                     IRPFWGDTGYKYIVIDNVYFLYEVSESETKVILLGTKFNNKRKTFNFANTKTQQKSMK
                     KWINDLAIYKPHKINQSQ"
     gene            19938..20855
                     /gene="nagK"
                     /locus_tag="PXD81_000229"
     CDS             19938..20855
                     /gene="nagK"
                     /locus_tag="PXD81_000229"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005708433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HDL5536300.1"
                     /translation="MAIYYGLDVGGTKIELAAFNEKLEKLHSERVPTPQTNYQDWLTA
                     IKTLVETADTKFDEKGTVGLGIPGFVNRETGIAEITNIRVADGKTILKDLAECLGREV
                     RAENDANCFALSEAWDESNKPYSTVLGLIIGTGFGGGFVFDGKIHSGRIGMAGEVGHM
                     QLNYHALKLLDWDKAPIYECGCGNQACLDTYISGRGFELLFNDLVGEKISAKAIIERF
                     YQQETKVVAFVEKYIELMAISISNLITILDPDMIVFGGGLSNFDYIYQALPKALPKHL
                     MRSAKVPVIKKAIHGDSGGTRGAAALFLK"
     gene            complement(20926..22875)
                     /locus_tag="PXD81_000230"
     CDS             complement(20926..22875)
                     /locus_tag="PXD81_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HDL5536301.1"
                     /translation="MYQRSQSRTVLFPILLFVGINLVILTLSRLVLGVWQSDRVSAVD
                     GWIPLILQGIRIDISSLCWLFGLPALFSVLCFHKNVLGSIWQTVLRIWLTVGSVFILF
                     MEVATPNFIETFDLRPNRLFIEYLVHPREVFTMLINGHLWAVICGIGIAMISLIIYWK
                     LSGWATKNLHFPSWKMRPLLALLVAAIVFVGGRSSFAHRGINPAMVAFSSDPLVNSLV
                     LNSGYSVLYAVQQLKDEDKSSEQYGKMSVEEMFNIVKQVRNRPASDYISDEIPTLTQN
                     KATYQGKPKNIVIILEESLGAQFVGSLGGKNLTPNLDKLSEQGWYFTNLYATGTRSVR
                     GIEAVTAGFTPTPARSVVKLTKSQTNFFSIAELLKRQGYHTSFIYGGEKHFDNMASFF
                     YGNGFQQIIDEKDYKNPKFTATWGVSDEDLFDKANETFNQLHKSGKPFFSLVFSSSNH
                     DPFEFPDGKIELYEQPKQTRHNAAKYADYAIGHFFELAKKSEYWQDTVFLVIADHDSR
                     AVGEHLVPIQHFHIPALLLGEHIQPRTDNRLVSQLDMPTTLLSVAGISGQYPMIGYDL
                     TQDIDPNRAFMQYDATQAMMRGNDVVVMRPNLPIEGFSYDKANYKLTPKEQPESFKKE
                     ALAHALLGSYLYKNQLYKLPEETKQ"
     gene            complement(22908..23696)
                     /locus_tag="PXD81_000231"
     CDS             complement(22908..23696)
                     /locus_tag="PXD81_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HDL5536302.1"
                     /translation="MQVTELNIYPIKSTQAIQVSQAFVEPKGLNFDREFMLAEPNGKF
                     ITARKDPELYRLAAFPIATGVMITHTSGQKCVALYQDFVEEQSSEVWGTHFNAKVAAE
                     AVNQWLSEIFGRAVQLRWLGSQSQRIVANFADHPMSFADSNPVSLMSLKSLEQVQQWS
                     PIPLEMARFRPNIVIDGNVAFEEEQWQQVQIGEVLFTKAALCTRCILITRDLNTLELD
                     PKSEPFRTLKEKHCDEKGKPVFGIHLVPQNSGVIKVGDPVIIQL"
     gene            23821..24051
                     /gene="xseB"
                     /locus_tag="PXD81_000232"
     CDS             23821..24051
                     /gene="xseB"
                     /locus_tag="PXD81_000232"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HDL5536303.1"
                     /translation="MAKKPTQDFESTLKELEAIVTHLESGDLPLEEALNEFETAIKLV
                     QQGQERLQKAEQRIQILLNKNDTAPLSDYERE"
     gene            24057..24950
                     /gene="ispA"
                     /locus_tag="PXD81_000233"
     CDS             24057..24950
                     /gene="ispA"
                     /locus_tag="PXD81_000233"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005617324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HDL5536304.1"
                     /translation="MSYLLATDLTHLQQRINHFLAEKLKTTDSVGSPLVEAMAYAVLL
                     GGKRVRPFLIYATGRMLGVPLEKLDHSAAAMEAIHAYSLVHDDLPAMDDDRLRRGKPT
                     CHIAFDEATAILAGDALQSFAFELLATDPHLTDREKVVQVTELATASGAKGMCLGQSL
                     DLIAENKAVSLEELELIHRNKTGALILSSVMMGLNLSEYSQNVAIKQPLVNYAKAIGL
                     AFQVQDDILDVIGETDKIGKIVGSDEHLNKSTYPKLLGLDGAKQKAEMLYQTALHSLE
                     QLPFDTTALKELANFIVKREN"
     gene            complement(24985..25785)
                     /locus_tag="PXD81_000234"
     CDS             complement(24985..25785)
                     /locus_tag="PXD81_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012262817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HDL5536305.1"
                     /translation="MCQLLGMNCNTPTDITFSFDGFRRRGGLTDHHTDGFGIAFFEGK
                     GVRVFRDNRPGASSPMAEAISQYQIKSLNVIAHIRKATRGDVNLENTHPFIRELWGEN
                     WVFAHNGTVEGVGVCEECHYQPIGTTDSEAAFCRIVSELKEMYPAKPSEKEIFDAVVQ
                     ITSRIAEHGVFNFVLSNGHWMIARCTTNLHYVCRKAPFGKALRDDDVSIDFSQYTKIT
                     DKVTIIATQPLTKNENWTKMKNGGYVFFKDGDVVYEIEGTLPEAQPHV"
     gene            complement(25910..26695)
                     /gene="znuB"
                     /locus_tag="PXD81_000235"
     CDS             complement(25910..26695)
                     /gene="znuB"
                     /locus_tag="PXD81_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005724066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HDL5536306.1"
                     /translation="MFDILFPAWLAGVLLAFITAPLGAFVVWRKMAYFGDTLSHSALL
                     GVALGIFLELDPYLAVIGMTILLALLLVWLEQKTNYSVDTVLGIIAHTSLSLGVITIS
                     LLDNVRVDLMSYLFGDLLAINFSDVVFIGIGATLICLILMLFWKRLLSITISPELAQI
                     EGLNVARLRLLLMILTALTIALSMKFVGALIITSLLIIPSATARRFAKTPEKMVLIAI
                     FFSIIAVSGGLLLSSWKDTPAGPSVVICAGLIFLLSLFKKEYH"
     gene            complement(26782..28119)
                     /locus_tag="PXD81_000236"
     CDS             complement(26782..28119)
                     /locus_tag="PXD81_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HDL5536307.1"
                     /translation="MSSLSFDFSEDFRPLAAKMRPRNLAEYVGQSHLIGEGKPLRRAI
                     EAGHSHSMIFWGPPGTGKTTLAEIIAHHLDAEVERISAVTSGVKEIREVIEQAKLNRQ
                     AGRRTLLFVDEVHRFNKSQQDAFLPHIEDGTIIFIGATTENPSFELNNALLSRARIYI
                     LKPLQAVEILKILQMAIADTERGLGNETLVLEDDVLALLADYVNGDARFALNCLELMV
                     DMAQDSAKGKVLNKSLLTEVLGERQARFDKGGDRYYDLISALHKSIRGSSADGALYWY
                     ARILTAGGDPLYVARRLLAIASEDIGNADPRAMQIAIVAWDCFTRVGAYEGERAIAQA
                     IIYLAVAPKSNAVYQAFNEAKRLAKEAKDYDVPAHLRNAPTQLMKSLGYGEEYRYAHN
                     EPNAYAAGENYFPPELKDTEFYFPSERGMEKQIKEKMAWLKAQDQASPIQRYK"
     gene            complement(28129..28617)
                     /gene="mreD"
                     /locus_tag="PXD81_000237"
     CDS             complement(28129..28617)
                     /gene="mreD"
                     /locus_tag="PXD81_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HDL5536308.1"
                     /translation="MRTNPIFQILVIIAIFVVAFVLEIMPWPVGFQGLRPTWVVLVLI
                     YWALALPDKMSVGTAFIAGIVWDLILGSILGIHALVLSVAIYFVAKYHLILRNLSLWL
                     QSLLVMAYIVLIRFFIFVIEYILHSAEFNSQELLGAVISGVLWSWIFLLMRHIRRQLR
                     LR"
     gene            complement(28617..29672)
                     /gene="mreC"
                     /locus_tag="PXD81_000238"
     CDS             complement(28617..29672)
                     /gene="mreC"
                     /locus_tag="PXD81_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HDL5536309.1"
                     /translation="MKPIFAKAPSLGMRLIIAVIFSIMLIISDGRSSLMIQARNLLET
                     AVSGLYYFANTPRSILDGVSGNFIDNNKLQLENNLLKEQLREKSADLLLLDQLKVENQ
                     RLRLLLSSPLRQDEYKKIAEVLAAEMDAYRQQVVINQGRLDGAFVGQPIIDERGVVGQ
                     VISVGEKSSRVLLLSDITHAIPVQVLRNDVRGIASGTGVNNELVIDNLPRSIDVVKGD
                     VLVTSGLGGRFPEGYPVAIVETVENDTKSQFARIVARPLASFDRLRYLLLLWPTAEEK
                     NYSQSLSPAQVREVVEERRNSVNPFERLKQMSNKKTEVQLEAKEEQIENDEINENPAA
                     QPEAENEQNYHTEEGAE"
     gene            complement(29774..>30727)
                     /locus_tag="PXD81_000239"
     CDS             complement(29774..>30727)
                     /locus_tag="PXD81_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005607179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HDL5536310.1"
                     /translation="DEPSVVAVRQDRMGSLKSIAAVGSHAKLMLGRTPKSIMAIRPMK
                     DGVIADFFVTEKMLQHFIKQVHSNNFMRPSPRVLVCVPAGATQVERRAIKESAIGAGA
                     REVYLIEEPMAAAIGAGLPVHEAMGSMVIDIGGGTTEVAVLSLNGIVYSTSVRIGGDK
                     LDEAIIAYVRRHFGSAIGEATAERIKQEIASAKIESDDDVKTMEVHGHNLAEGAPRTF
                     ELNSRHVLEAIEQPLNGIVEAVKSVLEQCPPELAADIFERGMVLTGGGALLHNLDRLL
                     SDSTGVQVVVAEDPLTCVARGGGKALEMIDMHGGDVFSIDD"
BASE COUNT         9119 a         6506 c         5877 g         9225 t
ORIGIN      
        1 aactcagaac ggaatatcat catcaaagtt atccattggt aaatcttgtg gatgtgttgc
       61 ttttggagat tggctcatag cttgatagtt agattgttgt ccatctacgc tatttgtagc
      121 attacggcta tctaacattt gtaatagttc accttggatt tcggttgtat aacgctcttg
      181 cccgttttgg tcttgccact tacgtgtccg taattttcct tcgatataaa ttttagagcc
      241 tttacgtagg tactgaccac aaatttcagc ataacggcga taaaaaacga tacgatgcca
      301 ttcagtgact tctcgacgtt ctccggtatt tttatccgtc caactttcag aagttgccac
      361 actaatatta gcaactgctt caccattagg catagtccgc atttcaggat cgttgccaag
      421 attaccgaca ataatcactt tattgattcc tgccatcgta tttactcctt caataaatta
      481 agttagttct attaaacttg attcagccta agaaaaaagt atttaattaa aatttggtta
      541 gagtgaattt ttcattttta cgtgtgcaat cacttattcc tccgttgtaa aggctttttc
      601 ttcgtgaagg taaggggctt gtacccttac ctttaaaagg ccttttaatg caggatttac
      661 aaactgcact ttttcaggga ttacaaaccg gattttcagg tattacaaac ttgaatatgt
      721 cctaattaac ggactgtttg ttttaccacg tcagcccacc gtggttggcg tttacacaca
      781 accaatatag gagttgttat atataaattt actacgaatt taagtctgat ttcgcaacgg
      841 cattatctac ctctttcaac ttttcagcac aatctgccaa ctgtcgcaaa atatgattaa
      901 acaccgaaat ctgcatatta atcaaaatcc tgtctttttc tactgcgaca acttttttcc
      961 tcacctccac tgggacagac ttatcatcta caatcggctg ccaaactgtg ccaccggttc
     1021 tggcattttg catactacgc cagcgtaaaa atgtaccgcc tgaggatgag gccttatcct
     1081 taatcatata aacagccaaa ggtgaaagat cctgccgcac agactgcacc atactatcct
     1141 gtgcagccat aaaataagcc cgagcttgag tgaactgttc ttgcaaaagt tcatagtctg
     1201 agccagtgtc aaaatggcta aatagctgtt tcagagaagt attcagccca gaatctttga
     1261 ctaattgccg ataaatttca tcagagatga ggttcatatc actccttatt tttgagattc
     1321 atttgtctgt gccgtgatat gggtttctac ggattgctct tcttcaacag tagtgttttg
     1381 cttgatgaag ggagcaaact cagcacgatg ctcaccggta agtacagcat ctgagagtgt
     1441 atagcccatt cgttttatcg cagcttgata gcgagcattc ttttcttgat agtcccgacg
     1501 agtgattcct gaactcttat aacgctcaac gatcccaaaa catttccgga ttaaccttgc
     1561 accagcttca atccaatctt gagcttctgc acgattgata atcgcaatat gagctgccgt
     1621 cattactgaa cggcaaaggc tatcaaaatc accaagtaag tagagtaatt gataccctag
     1681 ctgtgaatta acataaatcg ggtaagaaat cggggaaata ttggcacagc gttcaataga
     1741 aattccctcc ggcaacattt tggcgtagag cgtgacaatg tcgttggtga gcttttggat
     1801 ttccttccga taattgatca cactttcttc aaattgaatc agataatcgt cagcataagg
     1861 atcatcttct gatgcagccc gttgaatttg ggttagcatt gcaaagcaat ttggtacgct
     1921 gataatggag gattgagtta ctttgccttc ctcattacga atcattggtc gccccaccca
     1981 taatttatgg gcatattggg tatgtaacgt aaaagtgatc tcactacgaa gtggaccaag
     2041 ttgaggatct aattgagtgg tcatatatgc tccttgttta tttgattatt ttttagtctt
     2101 tggaaagggg ctttccattg tctgtttttt aatctttgca gacactgcct acgctgttta
     2161 ttttttatcc attgtgtacc tggtacacac tgattatttt tcactcacta ttaagcccct
     2221 taacagtgat tgttttttaa tcttattgta aaagtaattc agctctaaac ttctgcatac
     2281 gactagccat ttgctctctg tctgctgaag ttaagaccgg tttctcagta ggctgctcag
     2341 tcagtttttg tatagaatga gcagaattat tgttagcctg attctgtgag ggaagatttt
     2401 ttcctaattc aaaatagtag ggtttaaatt caccctgctt cgcccgctgg ataagcgtat
     2461 agagataacc tttcggtttt ttcacatcct gcttttgaat aatgcgttgt tgtacttcca
     2521 acaaaatagc ttggcataaa ccgaggtcta atcccttcat tgcctgtgca gtaagttttt
     2581 gttcaagtga agttaatggg atttcttgtg gccaagtaat ttcgctcggt tccccagtac
     2641 agtacttatt aatatataaa gtacggtact gtacatctga cttccctaat ggaagtaagt
     2701 ctaaaatcaa tgacttatct tcatttttag ccctaactcc cttattggaa gtctggtttt
     2761 tagaggattt tttcctgact tcctcatcaa aatccctaac ttccatagtg gaagttagga
     2821 tgttttgttg aattttgctt aattcttgtt ctgtttgcga tggctgttta tctttcacca
     2881 gctgctgttg ctggtatatc tgataacgac tttgtaaaca gtctaaatga gagacatagt
     2941 gccatttggt tttgtctgtt attagacttt caacaatatg atttgccaca ccattaataa
     3001 ggctatcttt atgtttggtg catttatcta aaaaagagag atagtcgcta tttaattgaa
     3061 ttgcatctaa aattggcaca ggttcatcat gtaggatata aacattacct tgtacataac
     3121 ctcgctcatc acgcactgtt tcacataaag taagccagcg agtaagacgg agtaaataga
     3181 gtgtttgagt cacgactttt tccgataatt tagcctccgc ataaggtcta tcggataaca
     3241 ttttgctgag cgtctcataa gagggaaata atcctccttg aaaggcttga acattgcact
     3301 taatgagctg ccaagcaaac tttgctcttg gtgtaaggca gggatcttgt aataatcgag
     3361 tagggactgt ttcgtgttgg ttgccaaaaa atagcaatcc atgctctgtt ggcttattcg
     3421 tgctgctaaa cattgtttct ctccaccagg ataaaagagt ttttctcttg ctattctctc
     3481 tcctatggct ataatgcacg ggacttctgc aaagtccttg taattcataa gatagagata
     3541 caagactcaa tatcgaatgc cccaaagctc caacttcggg gcatttattt tttacttatg
     3601 ttttgaatac cattcggaaa ccagtttcca aacaaccgtt agattcatat tgccttcctc
     3661 agcaatcaga catagaagct ctaatccttc ttgtgaatct aggatttcca ttcgttgttt
     3721 gtatctttgc caaatttccc aaatatgggt agattcttca tcacttgctg ccgcttttcg
     3781 tcccattttt tcttcgatac cgagtaattg acggcgagcc gagacttcag aggttgataa
     3841 accaaaatac tggtgcaaca tttcaataga ggctccgagc aataatgctc ggtcaataat
     3901 ttgtcgctgt tgagtattgg cttgtgcaat agccagtaat ttccaaaagg tttcgtgatt
     3961 aatctccacc ttagcaaaag agacaacgga gttgctaata tccataattt catcaatact
     4021 gagattattg atcgctttta gttgctcctc agaaaatccc atttttagac aaggatggat
     4081 atttccctct ttaatattga gcagggcgtt accaatcaat gcttcattta cggtaatcgg
     4141 cttaatcatt ttctcctcct tgttggcgaa tatggcgact gacccgaatg agacggaaaa
     4201 ttttgactaa ggtaagatca tcaagctcaa acaggaattt ggaatctaag acacagctat
     4261 tttcatcttt atgttcagca gtctgtaaca ttgagaggaa tgagtagagt gttaaagcag
     4321 acggtgaaag ttcagtatct gccggtttca ttgcataagt gtaatccact ggttgtttca
     4381 caatatgttc aaccagatgg tcaatgccca tactttcggc aatatctaag gccagtgagt
     4441 aggcctctga gcgatattga cgtgcggggt gaatcaacca aatatcttca accggttggc
     4501 gaccttcaca agcaaaagtc agtccattcg cttcagctcg ttgctctcgc tgttcttgaa
     4561 tactcattcc cggagtgagt ccaaagttat gggcaatttg ggatagataa tcttcttgat
     4621 tatcttggtt atcgagcaat gaagagccaa gctgagataa atcagtaata gcgttattct
     4681 gctcatcatc actttcgtta tcatcaatta cctcatcaat aatatgttga gctttttggg
     4741 gagtaacggc tttattttca tttttgatga gaggttcaga ttttgcagtt tgttgttcct
     4801 gtctggtatt gatttgatcg actgtttttt caactaaatt atcaatttct tgttgtttag
     4861 ccgctatttt tttgaatttt tgttcatcta agtcaatatc cagataaagt gtttcaaaac
     4921 tcactttacc ctctaaagct tcaaccattg ccgtaatcaa ttcactttgg aattcttttg
     4981 cttgaaatgg tgtagcctca ttaaatttgg ataaattttc tgaccagagc gattcaaaag
     5041 taacatcagt atcaaattga tgggctgccc atacctcttc agcattatta cgaatagcta
     5101 atagtttatc gatctgtgta tgaccaagac cactaaaaag aacttctggt atatgtggat
     5161 ataagtaatt aacacaacgt tccattttaa gaatgagagc atgagagata atatatccat
     5221 cattttctaa ctcagaagat aactctctcg aagaaagaga cttacctaat tttttctcgt
     5281 aatactcctt cgcctgcaaa atccccaatg ctttttcaat aaatgacaaa tccgcacggg
     5341 tatcattttc aatcaaatgc ccgataagta aattgagttc agcttcgaca ctgtcggcag
     5401 tatcaccttg ccaaggttta tattgacaac tgatggacca aaattttgga tctttggtct
     5461 cggtaaagag ctccttgagt gcctgaatac gagtattacc accgtcagcg ataatataga
     5521 aatcttctcc cggacgacgg gtgatattcg gcttatgatc taaccctcga cgacgaatag
     5581 actctttgat catctcaaag ttagggttac gggttttccg agggttatgc tcatacggac
     5641 gcaacttatc cagcgttact gtaatatatt caacatcgct actgtaatgc gttggtgtgg
     5701 ctgtttggta ggatggagcc gtatttgaaa taggtgaaac atttaatcct tttgctatcg
     5761 ctgccagtct tttttcatct tttgtcataa tctatcctaa tgcgttgtgt ttgttctgtt
     5821 ttcaaatagg gcaaattcac ttctgaaatt cgtcattacg gaagtaagtg gtccgtttct
     5881 ttgcttgccg ataatgattt cagcagtgcc tttaaacgga gaatccgcat cataaacttc
     5941 atctcggtag atgaataaaa tgacatcggc atcttgctca agtgagccgg attcccgtaa
     6001 atcagagttg atcgggcgtt tattggcacg ttgttccaca gaccggttca gctgagagag
     6061 ggcataaacc ggacaatcca tctcttttgc cagtaatttg agtgaacgag agatgttggc
     6121 aatttctaaa ttgcgatttt cataacgttg cgaggtttgc attaactgaa tgtaatcaat
     6181 gaaaatagct gccggtttgc catatttacg agcattttgt cgaactttag tgcgtaattt
     6241 ttgaggcgtg agactacctt catcatcaat aactaaacga tttttccaat gttctaggat
     6301 atagcccatt gctgaggtta gttttgacca ttcctcatca tccaaactgt ctgcctgtct
     6361 aatattttgc aatcccactc tggcacgcat agctagaaaa cgttgtaaaa tctgatctgc
     6421 cggcatttcc atgctgtgat agaacacagg agccgactcg aattttttaa gtgtatggta
     6481 agcgatagtt tgagaaaatg cggtttttcc catagaaggg cgggcaccaa tcacgattaa
     6541 atcgcctggc tgtccacctg ttgtcgcttc gtcaatacct tgaataccag ttggagtacc
     6601 tgtcacagga tctccatttt tagccgagag ttccatacga gagagcatat tggcaaaaat
     6661 atcacttaaa tctgctgcag attttgtatt atctgatagg ctaaactcag taattttttt
     6721 ctcaatgtca tcaacaacag cttcaaactt atctggcgtt ttcgcactct gtgtctgatc
     6781 aacaataaac tgccccagtt ttaaaagctg tcgctgttta ctatataaga gaacgatatc
     6841 cgtataagcg tggatattag ctgtagatgg agtgtttttg actatttctg caagataagc
     6901 aaatccgccc aaatcctcga tcactttttt ctgggtaagg tattgatcca atgtcaatat
     6961 atctgctggt ttaccggctt caatcaattc ggaaatatgc ttaaacagta gacggtgagt
     7021 aaaattataa aaatccccct cattgatttt gctactcact tcatcaaaga gttcattatc
     7081 caagagtaat ccaccgatta ctgaaaattc agcctctata gaatgcggct gtacaagtga
     7141 tttatttaag ctagacatac attctccctc tatgctgaga agtgagagtt tgcaaactgt
     7201 ggcatcagta acgcacaaag ctgactgatc gtttcatatt cagcttggct ctgacgatag
     7261 accggttttg aaaaagttgc cgcttcacga tatgccacgc ggtcaggtac cgaaaattcc
     7321 agcaatgttt tgttttcatc aaactcattt tggaagagag tatgaaggta gtcggaaacc
     7381 aatttgacat ctttagtgtg atcaacacag ttaatcatcg ctttcagcgg tggtagggta
     7441 aagccaaatg ccgtaaaggt ttcgaggtct tgatacattc caatcgtgcc acgaataaac
     7501 tccttagcag ataaaatgtg gggaagaata ggacagaata aaacatctgc tgccaataca
     7561 gacatatcga cagtaatatc tctggtacca cgagtgtcaa taatgatgac atcgtaacct
     7621 tcaattttct ggagcagtaa actaaaacgt aatgcaccgt caggtgaatc tctcagcatt
     7681 gggctgattt tatttgacgg gtcattagac tggatcaagt caagatttgg gatagtcgtt
     7741 ctagaaataa tgtgggtagg atctacatct ttaaaatgca gaaattcgta agttccaccg
     7801 ggagcttgat attccaactc ataataagag cttaatgtgg gttgggtatc ggtatcaata
     7861 agaagggttc ttagaccgtg ttctgcacag aatgcaccaa tgttagcagc attcgtactc
     7921 ttggcagagc cacctttagt tgaagcaata gtaatggtaa aaggtttgtt tgtaccgttt
     7981 tgtaatggtt tcataattta ctctcttaac aagggttaag gagtattggc cattaatacg
     8041 gtgtatttga attgattatt tgaaagagag aaactacaag tgtgattcaa atggtgcctg
     8101 aggtcggact cgaaccgaca cggttattca ccaacggatt ttgaatccgt catgtctacc
     8161 aatttcatca ctcaggctaa gagtgctttt tattcaatcg cctgtatgtc ctatcagttt
     8221 acgcataaga ttttgaatcc gctgcgtcta ccgatttcgc cacactggct taatttttgc
     8281 acgattatag ggaaatatga ccgcttgtgc aagcgtaaaa atgccttttg aaatcggttg
     8341 atttaaaaat cagcactcaa ttcatcttta caaaaatagt tatgcacatc acctgtggat
     8401 aactaagtga acaaccctgt ttataaatat ttaacctact gattttacta taaataattt
     8461 taaaaattgg cagcgttcaa aatttcccct acaaaaaatc cactacttga cttattattt
     8521 ttcctgtttt tagttgggtg ataatagcct caccacgttc aatattttct gttgaagtaa
     8581 gcgttttacc gtctttagtt tgggtaattg agtagcctct ggtcaagatt ttcaatgggc
     8641 taagtccatc aagtttagtg cataactctt gaaaatgtcg ctgctctttc agcattattt
     8701 tttcgataga ataagccaag cgttgcgaca tttttgccac catcgtttgt tgctgataaa
     8761 gttgataagg tagcgggttg cgttcaaacc gttgcgtaat gtttttccat ttttgctgtt
     8821 ttttgctgag ttgctgctcc atttgccaat ggagtttcag ctccaattgc ttaagcattt
     8881 gggattggcg ttcaatctgc ttttgcggat gttggtgaat taaacggaca tttagctctt
     8941 tccccacctg ctttttggct gcaacaagtg cataaattgc ctgctcgagg cggtattcca
     9001 tataatcaaa acgttgctgt tgcgattgaa tttgtttgct tggatgctga gcattcaggc
     9061 gtaaggggag ttgttggaaa cggttaaatt tatggttcca aactcggtca aaggctaaat
     9121 tcactttatc aaactgatgt tgtaattggc gtttcaattc attttgatct ctgctgacta
     9181 attccgcagc agccgaaggc gttggtgcgc gcaaatctgc aacaaaatca gcaatggtca
     9241 catcggtttc gtgtcccacc gcactgataa tcgggatttg tgaatgataa atggcataag
     9301 cgacactctc ttcgttgaaa caccataaat cctctaatga accgccacct cgcccgacaa
     9361 ttaatacatc acactcattg cggcggtttg ccagttcaat catattcaca atatcttgcg
     9421 ttgcttcttt gccttgcacg agagccgggt aaataaccac tgataagctc ggatctcgtc
     9481 gttgcaaaat atgtaaaata tcttgtaatg cggctccgct tgaggaggta ataataccaa
     9541 cggttttaac aaaagtagga attggctttt tatgctcttg agcaaacaag ccttgagctg
     9601 aaagttttgc tttaagttgc tcaaattgct gctgtaacaa gccttcgccc gccggttgca
     9661 tactctcaat aatgagctga tagtcacctc gtggctcata gagtgtaaca cttgcccgca
     9721 ccagcacctg cataccgttt tgtgggcgaa aattcactct tacatttttc attttaaaca
     9781 tcgcacaacg cacttgtgct ttgtcatctt ttaaagtgag ataccaatgc cccgaaactg
     9841 gttggctgaa atttgagatt tcgccggtta gccaaatatg gctcatttcc atttcgagca
     9901 aatggcggac ggaatagttg agttgggtaa cagttaaaat attgctcata ataatcaaaa
     9961 aagcgtaaat gtagtagaag tgtaacgaga aatgaaagcg agaggaagct tttgcagtca
    10021 acaagcggtt attttttgct gttttttggc aaataaaacg aaaaccctcg atgtgattca
    10081 ctatcgaggg tttggaatgt ggttgcgggg gccggatttg aaccagcgac cttcgggtta
    10141 tgagcccgac gagctaccaa gctgctccac cccgcgtctg atgaggctat aatatatcaa
    10201 tttgacataa tgacaagtaa aatcatacaa aattgctcta agtgatgtaa attacaacag
    10261 atatagtatc aaaatacgct tattgttgta aaaacaatca aattatattc tggttttatt
    10321 ttgtcgctgt ttttgctatt cttctgttta tttttcacat tttattaacg atataaggat
    10381 attcgatgaa ttggaaagca tataaaaaag ctattgcggc agctagcgtg gtagcgattg
    10441 caacggtatt agtggcgtgt tcgtcttcag gccaaaaagg caaaacaacc tcttatgatg
    10501 cggaaaaagc taaatttgga gcaaaatata atggtcgtca ctttatgcct tatggcacac
    10561 attttcaagc tggacgttta ggattaaatg ctgctcctgt taatcaacta gactttttac
    10621 agcagctttc taatgtcaga gcctatagta gcttggcaga ttcttatgct atcgcttacg
    10681 gaaaaatcag tcgttggatc tcatcgggcg gtaatattaa tgatttagca aaatatggta
    10741 ttagtactcg tttaatgcgt ggcgaagatg gctaccagaa tgtgttaatg acagggtatt
    10801 actcgccggt tatcaaagcc cgtagaacgc ctcaggggca ataccaacac ccgatttatg
    10861 cgatgccgtc tagcaaacgt ttttcacggg cggaaattta cgcaggagca ttgcaaggtc
    10921 agggcttaga actggcttat agtaattcaa tgctcgataa cttcttatta ggtgttcaag
    10981 gtagcggtta cgttgacttt ggtgatggtc gcttaaacta ttttgcttat gcagggcaaa
    11041 atggctttaa atatgccagc gttgggcgtt tattagtaga agatggcgaa attccgaaag
    11101 agaaaatgtc agtgcaggct atccgtgaat gggggcaacg taaccctagc cgtgttcaag
    11161 gtttgttgga gcgtaatcca tcgtatgttt tctttaaaaa cgatcctacc ggtcaggtaa
    11221 aaggctctgc cggtgtgccg ttagtaccac ttgcatctgt tgcatcagat cgtagcattg
    11281 tgccatcagg tagcgtgttg ttggtcgaaa tgccgttgat tgatagcaaa ggaaattgga
    11341 ccggcaaaca cgaaatgcgt ttaatggtgg ctcttgatgt tggcggtgca gtgaaaggcc
    11401 atcacttcga tttatatcaa gggattggcg ataaagcagg ccatatcgca ggtttaatga
    11461 aacattacgg ccgtgtttgg gtattaaatt aatacagggt aactatgtta aaaaaactat
    11521 ctgccatatt cttacttcta ccgagcgccg tgcttgcaaa taatttgcaa aatataaccg
    11581 cttcaactga gcctaaatat aaaatctcag aaatagatgt cagaattttg atacgccagc
    11641 tcaataatat tgagcaatgt atctatcctg agttagctaa acccggttac cagcaaatct
    11701 acgcaaattg gaatctggct gaaaatttaa cgatgcaata ttttgaatat cagcttctaa
    11761 aagagttgct cggagaggaa aatcagaaac tgatgcaaaa tgatacgctt tctaccgagt
    11821 atttccattt actgcattct cgtttaaatc accaaaaagc aaatgtagat cccgaaaaat
    11881 gcgatacatt taaacctaga tataaagaga tttataaaag tatggaaaat gcactcacta
    11941 agaaaaatca atagtaaaaa actcgcagtt ttcactgcga gtttctttta tctttcgtta
    12001 tgaatttcta aattggtggc ttgttttgcc gcctctgctt ttgctttatc gtttcgagat
    12061 cttgctatac tgtctaaata gcatttatct acatcacctg taatatattc tcctgtaaat
    12121 acagagtcat caaagcgatg aatagtcgga ttttccagtt gaatagactt ataaagtgct
    12181 tcaaggtctt ggaaaatcaa tttatccacc cctatcatct gtgcaacctc ttccactgag
    12241 cgatcataag ccactaattc ttcacaagtc ggcatatcaa tgccatacac attcgggtag
    12301 cgaatttccg gtgcggcaga ggcaaaataa acccgttttg caccagctgc tcgtgccatt
    12361 tccacgattt gttcagacgt tgtacctcgt acaatagaat catcaaccag taacacgctt
    12421 ttgcctttaa attcagaggc aatcgcattt aatttacggc gaaccgagct tttacgctgt
    12481 gcttgccccg gcataataaa agttcgagct acatagcggt ttttaacaaa cccttgacga
    12541 tagggtttat acaacatatt agcaatacgt accgcaatat catttgaggt ttcaggaatc
    12601 gggatcacca catcaatatc atcgataatt cgtccccatt cacgtttaat tttctcacct
    12661 aataattcgc ccatatgcac tcgtgcagaa tatacagaaa cgccatcaat gacggaatca
    12721 ggacgggcaa aataaacata ttcaaaaata caaggattca gttttggatt atcggcacaa
    12781 atttgcgaat gtaattgccc atcaaaggta acataaatcg cttcacccgg cagcacatct
    12841 cgcacaaatt caaaccccac tacatcaaga gccacacttt ccgaagcaaa catatattcg
    12901 gttttaccct cgatttcacg tttacccagc actaacgggc gaataccgaa tgggtcacgg
    12961 aaagcaacca taccgtgtcc gataatcatc gcaatgcaag cataagcacc acgaatgcta
    13021 tcattggttt tacgaaccgt ttcaaagata ttgtctgggc ttaaatgctg agtggaatag
    13081 agatctaaaa agtaagcaaa aatattaaga agggattcag aatcagaatt agtgttcaca
    13141 tggcggcggg cttcgttgta taagcgagct ttaagttccg cattattagt taaattaccg
    13201 ttgtgaacta aggtaatacc gaaaggtgaa ttgacataaa atggctgggc ttcagacaca
    13261 cttgagctac ctgctgttgg gtagcgaacg tgaccaattc caacattgcc ttgtaatctc
    13321 accatatgct cttgctggaa aacatcgctg actaagccgt tagctttgcg taagcggaaa
    13381 cgattttcat cgtctatggt gacgatacct gcggcatctt gtcctcggtg ttgaagtaat
    13441 gttaaaccat cataaatcgc ctgattcacc ggcgaattcc caataatacc gacaatgccg
    13501 cacatataga aaaacctctt tatttagtcg tgtttaaaaa actggaattt gcttgtaact
    13561 gttcaaagaa ccacttcaca ataaagtcaa aatgcggaat taatagcgat gatttccata
    13621 attccgtttg gcttgcttgg gtaaaggtat ctaagaaaaa taaaatagcc gacacaatta
    13681 aaataccacg caatgcccca aaagcaccgc ctaaaatacg gtcggtgata gacattccgc
    13741 tcaaatcaat cactttgcct aataaaccgt tgatcacacc acaaacaatt aaagtcaaaa
    13801 tgaacagaat cgccgctgca ataccattgc gtaaaaattc tgaattttgc aaataaaccg
    13861 aattaatttg tgtgagataa ccgcttaaat aaggataaaa cgagcttgcc acaaaaaagg
    13921 cgattaccca accaagtagc gataatgctt cattggtaaa acctcgccat aaactgataa
    13981 gcactgaaaa agcaatgatt ccgataacaa ttaaatcaac cataacacac cttaaagcgt
    14041 aaagctctcc tattctacaa aaaaagaaaa cgtttgcgta gcactatttc gctaaaaata
    14101 agtgctttat gcaaatacaa gcggttaaat tcggtaaaaa atttacacaa tccgaccgct
    14161 tgtaaaacct agtctgcaat ataaccaaca tcgtccaacg ttggattatt tgctccttgt
    14221 ttagccagct catcgcaaat ctcattttca cgatgtcctg aatgcccttt cacccaactc
    14281 cattcaattt ggtggcgttg aatttcttgg tctaaagcaa cccataaatc tttattttta
    14341 actggctttt tatcgctggt tttccagtca tttttcttcc agttaaaaat ccatttttgg
    14401 atcccgtttt tcatatattg gctgtcacta ttgagttgca ccttacaagg ctcttttagc
    14461 attgctaaag cctcaatcac cgcccgcaat tccattcggt tattggtggt ttgaaaatag
    14521 cctttgctca cggttttttc gtacccgtta taacgcagca aaataccaat cccccctttg
    14581 ccggggttgc ctaaacaaga accgtctgta aaaatttcaa ctaatttcat catcacgctt
    14641 tgaaaataaa agccccaaag cattaacttt ggggcagctt ttaggctaaa ttctaaaagt
    14701 tatcagaatg gagatcaact gattattttt ttccaagttg tggaatcacg ctattattag
    14761 ccgccaataa acctgcatcg gtataaactg ctaatttacc acgggtatcg acaatatcta
    14821 agttacgcat tgttaattga ccgatacgat ctgccggcgt aaacggtgca tcttctactt
    14881 tttccatact taaacgttct gcttcgtagg ttaagtttgg cgattcagtg ttaagaatag
    14941 tgaaatcgtt accacggcgt aactcaagcg tgaccgtacc ggtaatcgct tttgccaccc
    15001 aacgttgagc cgtttcacgc aacattaaag cttgcgggtc aaaccagcgg ccttgataca
    15061 ataaacgccc taaacgtaat ccgttaatgc gatattgctc aatcgtatct tcgttgtgaa
    15121 taccggtaac taaacgctcg taagcgatgt gtaataatgc cattcccggt gcttcataaa
    15181 taccacgaga tttcgcttcg ataatacggt tttcgatttg gtctgacatt cccaaaccat
    15241 gtctgccacc aatgcggtta gcctccataa ttaattcaac ggcatcatca atacgtttac
    15301 cgtttaacgc caccggcaca ccttcttcaa aggtaacaga aacagtttcc gctttaattt
    15361 caactgattc gtcccagaat gccacgccca taatcggttt tacgatcttc ataccggtgc
    15421 ttaattcttc taaatctttc gcttcgtggg ttgcacctaa catattcgag tctgtagagt
    15481 aggctttttc caccgacatt ttatagtcaa aaccgttttc gattaagaat ttagacattt
    15541 ccaagcgacc gcctaattca tcaatgaata gttggtctaa ccacggctta taaattttta
    15601 attttggatt ggttaataaa ccgtaacgat agaaacgctc aatatcgtta cctttaaagg
    15661 ttgaaccatc gccccagata ttcacatcat cttctttcat tgccgcaacc aacattgtgc
    15721 cggttaccgc acgaccaagt ggcgtggtgt tgaaatacgg aataccgccg gttgaaatat
    15781 ggaaagcacc gcattgaatt gcggcaatgc cttcgtgagc taactgttgg cgacagtcga
    15841 ttaatcgtgc attttcagcc ccatattcca tcgctttttt cggaattgcg ttgtaatcat
    15901 cttcatccgg ctgacctaaa tttgcggtgt acgcataagg cactgcccct tttttacgca
    15961 tccataacag tgctgctgag gtatctaaac cgcctgaaaa ggcgataccc actttctgcc
    16021 ctaaaggtaa agcttctaaa attgttgctg acatacgaat tcctataaat tttgaaagta
    16081 aaaatattca aacgcaaacg cttacgttca agcaaaatat tctagctgaa aagtagtaaa
    16141 aattcatcta aaatttagcc cgatgatttg caaatttttt gtgaaatttg accgcttata
    16201 atttactgaa aacctaaaag caatagagaa aatcagaatg aaaaaactac tcgtaatccg
    16261 taatgataaa attggggact ttatggtctg ctttccggca tttgctatgt tgaagcaatc
    16321 aatgccgaat gtggaaatta ccgctcttgt gccaagttat actgctccac ttgccgagct
    16381 ttgcccttct attgataaga ttattattga tacggcgaat aaaaaagata aaaatgaatt
    16441 taaccgtatt ttacaagcgg ttaaaaatga gcaatttgat gcggttattt gttttgtttc
    16501 agactggcat aatgccaaac tcacctggca aagtgggatt aaataccgcc tcgctccagc
    16561 aactaaatta ttccaatttt tatataacca ccgtttaacc caacgccgtt ctcaatcagc
    16621 aaaagcggaa tccgaatata atttggattt agcccgtgct tttttaagca aacacaatat
    16681 accgattgtt gaacctcaca cgccatactt aagtgttgaa cagcaaaccg tccaggcaca
    16741 aaaagcaaaa ttatgcgaac aactcaatat taccaccgtt aaaaagtggg tatttgtgca
    16801 tagttcaacc ggtggttcgg caaataattt atctatagaa cagtatgcag agttaattca
    16861 aggtattttg gcagaatttg attgctatgt ggtactcacc gcagggaaag gcgaaagcga
    16921 aaaagctcag cagttagctc aacaaatcaa tcacaacaac gtggtggttt atgataaaaa
    16981 tgaggggtta caagatttta cacattcgct tgcgtgtgca gatttgttta ttgccggctc
    17041 aaccggtccg ttacatatta gtggggcgtt aaatattcca actattggct tctacccgag
    17101 ccgactttct gccattccac gccgttggaa accgattaat gatgcgaata aacacattgc
    17161 attttgccca ccgtctgata aagcctcaca aatgaattta actttaattt ctatttctga
    17221 ggcattaaaa acgatagtac cgtttattcg gagaatgtgg aatactccct ctctctgata
    17281 aaattgctaa acgcaatttt atctatctct ctcccactag agggagagag tactgaaaag
    17341 catcatttcc gagtacgatt ccacaaatcc gcatatttta cgaaagtaga atgtgccgaa
    17401 agaactgcca ataaaaatcc ttgcttgcca tctaaaaaac cggcttttaa tagatacatt
    17461 ttcacaaaac agccgatggc gtgagtcacg ccatcaaata agctggctgt tttgcctgca
    17521 tttgcccgtt ggattgccca cgctttagcg taacttgccg attttaccaa atagtggtga
    17581 atatctttat aggtgaaatg cagcaaatcg ccttgtagtt tctggatttt tgcctctttt
    17641 gggtaatgta ctttttcgtg aaccaattca tcaccatatt tggcaaattc cgtgcgatac
    17701 aatctcacca cacaatccgg ataccaaccc gagtggcgaa tttgtttgcc aaatacttca
    17761 cttaagcggc tgattttgta aacggtattt ttttcatcat ttttgaccgc ttgtaaaata
    17821 gattgcttca gttcggctgt cactcgctca tctgcatcaa gccaaagtac ataatcactg
    17881 gtcacatatt gttgagctaa ctgacgctgt ttaccaaagc cttgccaatc ggtgttagtg
    17941 tagaaccttg cgttgtattc agcagcaatt tgtggtgttt catcagtact gccactatct
    18001 aaaatcacaa tttcatctac ccaatctttt actgtgtcaa ggcaagcccg taaatcttga
    18061 gcttcatttt tgacaatcat cgcaacactt aatgtcggca tagtttatcc ttctctcttt
    18121 ttgtcgtgtg tttttctgct attcgttgca ttttacttta aaaatagtgg tttttgtgct
    18181 attttctacg ccaaatttta tcaacaaaac aaattaacgg aaaaagaatg tcaattacat
    18241 taacaaccaa acaaaaacaa ttcctaaaag gcttggctca tcatttaagc ccagtggtga
    18301 tgctgggggg taacggctta acagaaggcg tgttagctga aatcgataac gccttagacc
    18361 accacgaact gattaaagtt aaagttgccg gggctgatcg tgaaaccaaa caattaatta
    18421 ttgatgccat tctgcgtgaa actaaagcgg ctgaagtaca aaccattggg cacattttag
    18481 tgctttaccg ccaaagcgaa gagaaaaaaa tcagcttgcc aaaagcaact aaagcgatct
    18541 aattgcaaaa atatgtttaa attcaaccgc ttgtattcaa gcggtttttt ataggacttt
    18601 ctatgtttaa aaaattctct ctttataccc ttgcaatgtt gctcattcct gtggtgactt
    18661 ggctggcaaa ttggcactgg tctctggcaa ttcaatacgc tgaatttgat ttcgactact
    18721 ttttatattt aatcaccgaa accggctccg taccttatgc catcatcacc tgtgtggttt
    18781 tcgccttatt actggcgagg gtggttcgca aacgctactc tttaattttt gtgattaccg
    18841 tgtgtgtcgc ttcaatggta gccactcaag caatcaaaac agcgatgaaa tcaacctttc
    18901 aagagcctcg tccatttgtc actgctcttt tccaagataa aacagacgat ttttatcaat
    18961 taacacgcga gcaacgtggg cttgccgtaa tggaaaaaat cggtgcaaat caaaattttg
    19021 ttaccgctca ccaagccaaa gaagtgggct attctttccc atcaggccac actatttttg
    19081 cggtttcttg gctattattg gtggtaggtt tctgccgtaa tatgcgagga caagcggtcg
    19141 ttttggctca tttttttgca atcagttggg cggcgttaat gctgattagc cgcttgcgtt
    19201 taggtatgca ctacccgatt gatttattca ccagtagttt aattgcgttt ctaatcaatt
    19261 tagccttatt tgtgtggttt gtgccttggt tggaaagtaa aaaatggttt gctaaaaagc
    19321 cttaattggc aatttagcca aatcaatata caatacaaat tattgaataa gtacctaaaa
    19381 taaggagctt agaatggaaa tgtcgataga aaaaatagct gaactattaa gcgataaaga
    19441 aaaagatgac tatccgttta gtattgagga aagacaattt gccgcccagg ctagtgctga
    19501 tacttgggag gcaatcttaa ataacactat tcaacctttt gacttcaaaa aataggcttg
    19561 ctatgtatca agtttttatt gcagaaccaa aagacccaaa cttagagaaa ggcttattga
    19621 aacacttcat taagtcttat cttactcaag aagaactcga tttcctgcat aaaattctca
    19681 ctaatttacc caacatcaga cctttttggg gcgataccgg ttacaaatat atcgtcattg
    19741 ataatgttta cttcttgtat gaagtttccg aaagtgaaac gaaagtcatt ctacttggca
    19801 ccaaatttaa taataaacgg aaaactttta attttgcgaa tacgaaaacc cagcaaaaat
    19861 caatgaagaa atggattaat gatttagcaa tttataaacc gcataaaatc aatcaatcgc
    19921 aataagagag cgtaattatg gcaatttatt atggcttaga tgtgggcggc acaaaaattg
    19981 aattagccgc ttttaatgaa aaactagaaa aactacacag cgaacgtgtg ccgacaccgc
    20041 agacaaatta tcaagattgg ctgacggcaa ttaaaacgct ggttgagact gccgatacaa
    20101 aatttgacga aaaaggcact gtcgggctgg gcattccggg ctttgtaaac cgtgaaaccg
    20161 gcattgcgga aattaccaat attcgagtgg ctgacggcaa aacgattctg aaagatttag
    20221 ccgaatgttt agggcgtgaa gtgcgtgccg aaaacgatgc taactgcttt gccctgtctg
    20281 aagcgtggga cgaaagtaat aagccttatt ccaccgtatt agggttgatt atcggcaccg
    20341 gttttggcgg tggttttgtg tttgacggta aaattcattc aggcagaatc ggaatggcag
    20401 gcgaagttgg gcatatgcaa cttaactacc acgcacttaa attgctcgac tgggataaag
    20461 ccccaattta tgaatgtggt tgtggcaacc aagcctgttt agacacctat atttcagggc
    20521 ggggttttga gttactgttt aatgatttag taggagaaaa aatctcggcg aaagcgatta
    20581 tcgaacgctt ctaccaacaa gaaaccaaag tggtggcatt tgtcgaaaaa tatattgagc
    20641 taatggcaat cagtatcagc aatctgatta cgattttaga tccggatatg attgtattcg
    20701 gtggcggttt atccaacttt gattatatct accaagctct accaaaagca ttaccaaaac
    20761 acttaatgcg ttcagcgaaa gtgccggtca tcaaaaaagc aattcacggc gactcaggtg
    20821 gcactcgtgg tgcagcagca ttattcttaa agtaatgaac aaatttgaga gagggcaagc
    20881 ggtcattttt aataaaaata ttgcagccct ctcttttaat ttgccttact gcttagtttc
    20941 ttccggtaat ttatataact gatttttata taaataactg ccaagcaatg cgtgtgctaa
    21001 cgcctctttt ttaaacgatt ccggctgttc tttaggggtg agtttatagt tagctttatc
    21061 gtagctaaat ccttctatcg gtaaatttgg tcgcattacc accacatcat ttccccgcat
    21121 catcgcctgt gtcgcatcat attgcataaa ggcacggttt gggtcaatgt cttgcgttaa
    21181 atcataccct atcatcggat attgaccact tattcccgcc acagaaagta atgttgtcgg
    21241 catatcgagc tgactcacta aacggttatc cgttcttggc tgaatgtgtt cgcctaataa
    21301 aagtgccgga atatggaagt gttgaattgg cactaaatgc tcgccaacgg ctcttgaatc
    21361 gtgatctgca atcactaaaa acaccgtatc ttgccagtac tctgattttt tcgctaactc
    21421 aaagaaatgc ccgatagcat aatcggcata ttttgccgca ttatggcgag tctgcttcgg
    21481 ttgttcatag agttcaattt tgccgtccgg aaattcaaac ggatcgtggt tactggaact
    21541 gaataccaaa ctaaaaaacg gtttacctga tttgtgcagt tgattaaaag tttcatttgc
    21601 cttatcaaac agatcttcat cactcacgcc ccaagttgcg gtaaatttag ggtttttata
    21661 atctttttca tcgataattt gctggaaacc attaccgtag aaaaagctcg ccatattatc
    21721 aaaatgcttc tccccaccat aaataaagga agtatggtat ccttgacgtt ttaataattc
    21781 ggcaatacta aaaaagttag tttggctttt cgtcagtttt accacagatc ttgccggtgt
    21841 tggtgtaaaa ccagccgtta ccgcttcaat tcctctcacc gaacgagtac ctgttgcata
    21901 aagattggta aaataccagc cttgttctga aagtttatct aaatttggag tgagattttt
    21961 ccctcctagc gaaccgacaa attgagcccc taaactttct tctaagataa tcacaatgtt
    22021 tttaggcttt ccttgataag tcgctttatt ttgcgtgagg gtgggaattt catctgaaat
    22081 ataatctgaa gctggtcgat tacgaacctg cttaacaatg ttgaacattt cctccacaga
    22141 catttttcca tactgttcgg aagatttatc ttcatctttc aattgctgaa ccgcataaag
    22201 cacggaataa cctgaattta gcaccagtga attgacaagc ggatcgcttg aaaatgccac
    22261 cattgccgga ttaatccctc gatgggcaaa actggaacgc ccaccaacaa aaactattgc
    22321 agcaaccaat aaagccagta gcggacgcat tttccagctc gggaaatgca aatttttggt
    22381 tgcccaaccc gataatttcc aataaatgat gagagaaatc atcgcaatgc caatcccaca
    22441 aataacagcc cataaatgtc cgttaatcag cattgtgaac acttctctcg gatggaccaa
    22501 atactcaatg aaaagacgat ttgggcgaag gtcaaaggtt tcaataaaat tcggtgtggc
    22561 gacttccata aaaagaataa atacactacc gacagttagc caaattctta aaacagtctg
    22621 ccaaatcgaa cccagtacat ttttatgaaa acacaacacg ctaaataatg ccggtaagcc
    22681 aaatagccaa catagcgaag atatatcaat gcgaatacct tgtagtatta atggtatcca
    22741 tccatcaaca gcggatactc gatcagattg ccaaacgcct aacactaaac gggataaggt
    22801 gaggataact aaattaatcc caacaaatag taggattgga aatagcacag ttcttgattg
    22861 actgcgttgg tacattttga atctcctttt aaaaaaatat cggcatatta taattgaata
    22921 atgacaggat cgcctacttt tatcacacca ctgttttgtg gcacaaggtg aataccgaac
    22981 accggcttac ctttttcatc gcaatgcttt tcttttaagg ttctaaacgg ctccgatttg
    23041 gggtctaact caagcgtgtt taaatcccgt gtaatcaaaa tgcaacgagt acataatgcc
    23101 gctttggtaa atagcacttc gccaatttga acttgttgcc actgctcttc ttcaaatgcc
    23161 acattgccat caatcacaat attcgggcga aaacgagcca tttcgagtgg aatcggcgac
    23221 cattgttgca cttgctcaag cgattttaga gacatcaaac tcaccgggtt gctatctgca
    23281 aaactcattg ggtgatcggc aaaattcgca acaattcgtt ggctttgcga gccaagccaa
    23341 cgtagttgca cagccctacc aaaaatttca ctcagccatt gattgaccgc ttctgccgcc
    23401 actttggcat taaaatgtgt tccccacact tcactactct gctcttccac aaaatcttgg
    23461 taaagtgcaa cgcatttttg accgcttgta tgggtaatca ttacccctgt cgcaatcggg
    23521 aaagccgcta gacggtataa ttccggatct ttacgggcag taataaattt gccgttaggc
    23581 tcggctagca taaattcacg gtcaaaattt aagcctttcg gttcaacaaa tgcctgtgaa
    23641 acttgaatag cttgagttga ttttatcggg taaatattga gctcggtaac ttgcatagaa
    23701 aatcctccat ttggaaatag gataacaata cctgcaagcg gtcgaaaagt aaaaggaatt
    23761 tgcaaatccc ttgtgagaac ggtaaacttg agcaactttt acagcaatag gtcagcaaca
    23821 atggcaaaga aacccactca agattttgaa agcacactta aagaattaga agcaatcgtt
    23881 acccatttgg aatccggtga tttaccgctt gaagaagccc tcaacgaatt tgaaactgca
    23941 atcaaattag tgcaacaagg gcaagaacgg ttacaaaaag ccgagcaacg tattcaaatc
    24001 ttattaaata aaaacgatac tgccccactt agcgattacg aaagagaata atcacaatga
    24061 gctatctatt agcaaccgat ctgactcacc tgcaacaacg gattaaccat tttctcgctg
    24121 aaaaattaaa aacaacggat agcgttggtt cgcctttagt agaagcaatg gcttatgcgg
    24181 tattgctagg cggtaaacgg gtgcgaccgt ttttaatcta tgccaccggc agaatgcttg
    24241 gtgtgccgtt ggaaaaatta gatcatagtg cggcggcaat ggaggctatt cacgcttact
    24301 cgctggttca cgatgattta ccggcaatgg acgatgatag attacgccgt ggcaagccaa
    24361 cctgccatat tgcctttgat gaagctaccg ctattttagc cggtgatgcg ttacaaagtt
    24421 ttgcgtttga actgctggca accgatccgc atttgaccga ccgagaaaaa gttgttcaag
    24481 ttacagaatt ggcaaccgct tccggtgcaa aaggaatgtg cttagggcaa agtttggatt
    24541 tgattgcgga aaataaagcg gtgagcttgg aagagcttga attaattcat cgcaacaaaa
    24601 ccggggcatt gattctatca tccgtgatga tgggattaaa cctttccgaa tattcgcaaa
    24661 atgtagccat aaaacaaccg cttgtaaact atgccaaagc gattggctta gcctttcagg
    24721 tgcaagatga tattttagat gtcattggcg aaacagataa aatcggcaaa atagtgggtt
    24781 cagatgaaca cttaaataaa agcacatacc cgaaattgtt gggcttagac ggggcaaaac
    24841 aaaaagccga aatgctttat caaaccgctc tccattcgct ggaacagttg cctttcgata
    24901 ccaccgcatt aaaagagtta gctaatttta ttgttaagcg tgaaaactaa gaaaaaacct
    24961 agccgaagct aggtttttta gtgattaaac gtgaggctga gcttccggca atgtgccttc
    25021 aatttcatat accacatcgc cgtctttaaa gaaaacatag ccaccgtttt tcattttggt
    25081 ccagttttca tttttggtaa gtggctgagt ggcaataatc gtcactttat cggtaatttt
    25141 ggtatattga ctaaaatcaa tcgacacatc atcatcacgc aaggctttgc caaacggtgc
    25201 tttacggcaa acgtaatgca gattagttgt acaacgggca atcatccaat gaccgttgga
    25261 taaaacaaaa ttaaataccc cgtgttccgc aattcttgag gtgatttgca ccacggcatc
    25321 aaaaatctct ttttcgcttg gtttagccgg atacatctct tttaattccg acacaatacg
    25381 gcaaaatgcc gcttcggaat cggttgtacc aatcggctgg tagtggcact cctcgcatac
    25441 cccaacacct tccactgtgc cgttatgggc aaacacccag ttctcgcccc ataattcacg
    25501 aatgaagggg tgggtatttt ccaaattcac atcaccacgc gtagctttac gaatatgtgc
    25561 aataacattg agcgatttaa tttgatattg gctgattgct tctgccatcg gcgatgatgc
    25621 tcccgggcgg ttatcacgaa aaacccgaac ccctttgcct tcaaaaaagg caattccaaa
    25681 cccatcagta tggtgatccg tcagcccacc ccgacggcga aatccatcaa aagaaaaagt
    25741 aatatcggtt ggcgtattac aattcattcc aagtaattga cacataattt gcttcttaaa
    25801 accaaataat ttacaacaaa aaataattgt tacatttaaa cagattgatt agaaaattca
    25861 atgctcttta cataaaaatc ttatgcttta ttttcataca ttatgctgtt taatggtatt
    25921 cttttttgaa tagcgataat agaaaaatca agccggcaca aatgactacc gaaggccctg
    25981 ctggtgtatc tttccaactg gataacaata aaccgccact gactgcaata atactgaaga
    26041 aaatcgcaat caacaccatt ttttcaggcg ttttagcaaa gcgtcttgcc gtagcagacg
    26101 gaataatcag cagtgaggta ataattaaag caccgacaaa tttcatactt aatgcgatag
    26161 ttaatgcggt taaaatcatc agcaataaac gtaagcgagc cacatttaat ccttcaattt
    26221 gagcaagttc cgggctgatg gtaatcgaca acaaccgctt ccaaaacagc attaaaatca
    26281 aacaaattag ggttgctccg attccgataa acaccacatc agaaaaatta attgccagta
    26341 aatcgccgaa taagtaagac atcaaatcca ctcgtacatt atccaacaaa ctaatggtaa
    26401 ttaccccgag agaaaggcta gtatgggcga taatccccaa cacggtatcc accgaataat
    26461 tggttttttg ctccaaccaa accagcaata atgcaagaag aatagtcatt ccaattacag
    26521 caagatacgg atcgagttct aaaaaaatcc ctaatgccac gccgagcaat gcagagtgcg
    26581 aaagtgtgtc gccgaaatac gccatttttc gccacaccac aaaagcccct agaggtgcgg
    26641 taataaaggc taacaaaaca ccggcaagcc aagccggaaa taaaatatca aacatattgg
    26701 ttctcaaagg caagtaaaac aagcggttat tttttcggaa aaatttgcaa attttttgct
    26761 gatttagacc gcttgtcata tttatttata acgttgaatc gggctggctt gatcctgtgc
    26821 tttcagccac gccatttttt ctttaatctg tttttccatt ccccgctcac ttgggaagta
    26881 gaattcggta tctttcagct caggcgggaa ataattctcc ccggcggcat aagcgtttgg
    26941 ctcattgtgg gcatagcgat actcttcgcc atagcctaaa gatttcatta attgagtggg
    27001 agcattacgc aaatgcgccg gcacatcgta gtcttttgcc tctttagcga ggcgctttgc
    27061 ttcattaaaa gcttgataga ccgcattgct ctttggggca actgccaaat aaatgatcgc
    27121 ttgggcaatc gctctttcac cttcataagc ccccactcgg gtaaagcaat cccaagccac
    27181 aatcgcaatt tgcattgctc gagggtcggc attaccaata tcttcagaag caatcgcaag
    27241 cagccttctt gccacataaa gcggatcacc accggcggtt aaaattcggg cataccagta
    27301 taatgctcca tcagccgatg agcctcgaat ggatttatgt aacgcagaaa tgagatcata
    27361 gtaacgatca ccgcctttat caaaacgagc ttgtcgttcg cctaacactt cggtcagcaa
    27421 acttttattc agcactttcc ctttagctga atcttgtgcc atatctacca ttaattccaa
    27481 acagttcaag gcaaaacggg catcaccatt aacataatca gcaagcaatg ccagtacatc
    27541 atcttcaagt accaaagttt cattgcctaa accacgttcg gtatcggcta tcgccatttg
    27601 caaaattttt agaatttcga ccgcttgtaa cggctttaaa atataaattc tggctcggga
    27661 aagcagtgcg ttatttagct caaaagaggg attttcggta gtcgcaccaa taaaaataat
    27721 agtgccgtcc tcaatatgcg gtaaaaacgc atcttgctgg cttttgttaa agcggtgaac
    27781 ttcatccaca aaaagcaacg ttcgtctgcc ggcttggcgg tttaatttgg cttgttcaat
    27841 cacctctcgg atctctttta caccgctggt cacagcggaa atacgttcaa cttccgcatc
    27901 aagatgatgg gcgataattt cagctagggt cgtttttccg gtgccgggcg gtccccaaaa
    27961 aatcatagaa tgactatgtc cggcttcaat cgctctgcgt aacggtttgc cttcgccaat
    28021 taaatgagat tgcccgacat attcagccaa gtttcgaggt cgcattttgg ctgcaagcgg
    28081 tcgaaaatct tcactaaaat caaaagacaa actactcatt ttaacctctt aacgtaaccg
    28141 cagttggcga cgaatatgac gcattaataa gaaaatccaa gaccaaagca caccactgat
    28201 tacagcacct aacaattcct gtgaattaaa ttcggcactg tgtaaaatgt attcaatcac
    28261 gaaaataaag aaacggatca gcacaatata tgccattact aataggcttt gtagccatag
    28321 cgataagttt cgcagaatca gatggtattt cgcaacaaaa taaatcgcca ccgataacac
    28381 taaggcgtgg atgcctaaaa tcgagccaag aatcagatcc cagacgatgc cggcaataaa
    28441 agccgtcccc acactcattt tatcaggtaa tgccaatgcc caataaatta acaccaaaac
    28501 gacccaagta gggcgtagcc cttggaatcc taccggccaa ggcataatct caagtacaaa
    28561 agcaaccaca aaaatcgcaa ttatcacaag gatctgaaaa atcgggtttg ttctcattat
    28621 tccgctcctt cttccgtatg atagttttgt tcattttccg cttctggttg tgctgccgga
    28681 ttctcattta tctcatcatt ctctatttgt tcttcttttg cctctagctg cacttcagtt
    28741 ttcttgttag acatctgttt taaacgttca aatggattca cgctattacg tctttcttca
    28801 acaacttcac gtacttgcgc tggcgacaag ctttgggagt agtttttctc ttccgccgtc
    28861 ggccaaagca gtaataagta acgtaagcga tcaaatgagg ctaacggacg agcaacaatt
    28921 ctggcaaatt gacttttggt atcattttca accgtttcta caatcgccac cggatatccc
    28981 tcagggaaac gtccacctaa gcctgaagtc accagcacat caccttttac cacatcaata
    29041 gaacgaggca agttatcaat gaccaattca ttattcaccc ctgtaccact ggcaatgcca
    29101 cgtacatcat tgcgtagcac ttgaacagga atagcatgag taatatcaga gagcaataaa
    29161 acacggctag atttttcccc tacagaaata acttgtccaa caacacctct ttcatcaata
    29221 ataggttgcc cgacaaaagc accgtctaaa cgcccttgat taatcactac ttgctgacga
    29281 taagcatcca tctcagcagc taatacttca gcaatttttt tatactcatc ttgccgaagc
    29341 ggagaactca ataacaatct taaacgctga ttttcaacct ttagctgatc aagcaataat
    29401 aaatcggcac ttttttctct tagctgctct tttaataaat tattctcaag ctgtaattta
    29461 ttattatcaa taaaattgcc acttacccca tctaaaattg aacggggggt attagcaaaa
    29521 taatataatc cacttaccgc ggtttctaat agattacgcg cttgaatcat taaagagcta
    29581 cgcccatcgg aaataatcag cataatagaa aatatcactg cgataatcag gcgcatccct
    29641 aaagagggag ctttagcaaa aataggtttc attagagaaa tatatgtgaa tttattggga
    29701 atctataacg aaaattgaca atagaatcaa tccattgcca acctccattt aaacagctgc
    29761 taagagaaat gtattaatca tcaatactaa atacatcacc accgtgcata tcgatcattt
    29821 ctaatgcttt accaccacca cgagctacac aagttaatgg atcttctgct actacgactt
    29881 gcacgccagt tgaatcagaa agtaaacgat ctaaattatg taataatgca ccacctcctg
    29941 ttaacaccat accgcgctca aaaatgtctg ctgccaattc aggtgggcat tgctctaaga
    30001 ctgatttaac tgcctctaca ataccattta atggttgttc aattgcttct aatacatgtc
    30061 ttgagttaag ctcgaaagta cgaggagcgc cttcagctaa attgtgtccg tgtacttcca
    30121 ttgttttaac atcatcatcg gattcaatct tagcactagc aatttcttgt ttaattcgct
    30181 ctgccgttgc ttcaccaata gcagaaccaa aatgacgacg tacataagca ataatagctt
    30241 catctaactt atcaccacca atacgcacag aagttgaata cacaatgccg tttaaagaaa
    30301 gcactgccac ttcggttgta ccgccaccaa tgtcaattac cattgacccc attgcttcgt
    30361 gaaccggcaa acctgcaccg attgcagccg ccattggctc ttcaattaaa tagacttcac
    30421 gcgctcctgc tccaattgca gattctttaa ttgcacggcg ttccacttgt gttgcacctg
    30481 ctggcacgca cactaataca cgagggcttg ggcgcataaa gttattacta tgtacttgtt
    30541 taataaagtg ttgcaacatt ttttctgtta caaaaaaatc cgcaatgaca ccatctttca
    30601 ttggacgaat cgccattatg ctttttggtg tacggcctag cattaatttt gcatgtgagc
    30661 ctaccgccgc aatacttttt agcgatccca tacgatcttg acgtacagca accactgagg
    30721 gttcatc
//
LOCUS       DANLYI010000007        28896 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.007, whole genome shotgun sequence.
ACCESSION   DANLYI010000007 DANLYI010000000
VERSION     DANLYI010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 28896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28896
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.007"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            <1..235
                     /locus_tag="PXD81_000240"
     CDS             <1..235
                     /locus_tag="PXD81_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009874565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL5536311.1"
                     /translation="LQIMPWFYALQWLSTITAFLYLVIFATITVYANTALSNMLVKLD
                     NGKNQGLMFGSSKLILAITTLGVMNVLPYIFLI"
     gene            complement(232..1365)
                     /locus_tag="PXD81_000241"
     CDS             complement(232..1365)
                     /locus_tag="PXD81_000241"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HDL5536312.1"
                     /translation="MLIFMMNEYSPNYYIGVMSGTSLDGVDIALLDFAKNPPKMTACD
                     FFPMPEELRADISALLKTGETNLQKLGEIDHRLGLLYAETINAFLAKNQLKAEDIQAI
                     GCHGQTVWHSPDSEYPFTTQIGDMNLVAEQTGITTIADFRRKDMAVGGQGAPLVPAFH
                     QAIFSDPNRLTVVLNIGGISNISVLNPNSPTVGYDVGAGNTLMDSWIEQHQGKRYDKN
                     GEWARTGKLNTELLATLLDESFFAKAPPKSTGRELFNLTWLAKKLENLTACQPQDVQR
                     TLAEFTAQSIANELLRFESSLPRLLLVCGGGSRNPLLMERISALLPEWEVTTTTEFGL
                     DIDYVEAAAFAWLAYQRIHNLPSNMPSVTGAKKAVSLGVIYPA"
     gene            complement(1557..2396)
                     /gene="queF"
                     /locus_tag="PXD81_000242"
     CDS             complement(1557..2396)
                     /gene="queF"
                     /locus_tag="PXD81_000242"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005623076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HDL5536313.1"
                     /translation="MNYNDKSLSALKLGQKTEYKSEYDASLLQPVPRKLNRDSLGITQ
                     EQPFNRGADVWTCYELSWLNQNGLPQVAIADVAIDFKSENLIESKSFKLYLNSFNQTK
                     FSSIEEVGKTIQADLARCASGQVSVKIYQLSHYTQQPIVDFAGDCIDEQDIQIDSYAF
                     STHYLENIAEDELVEETLVSHLLKSNCLITSQPDWGSVQIRYKGKKLNREKLLRYLVS
                     FREHNEFHEQCVERIFCDLMQFAEPEKLTVYARYTRRGGLDINPFRSNFEEVPENLRM
                     ARQ"
     gene            complement(2401..2949)
                     /gene="gmhB"
                     /locus_tag="PXD81_000243"
     CDS             complement(2401..2949)
                     /gene="gmhB"
                     /locus_tag="PXD81_000243"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HDL5536314.1"
                     /translation="MGKKAIFLDRDGTINIDHGYVHQIDDFQFIDGVGKALKQLQDKG
                     YLLVLVTNQSGIARGYFSEAQFMQLTEWMDWSLDEDYGVVLDGIYYCPHHPEGKGEYR
                     KDCDCRKPKAGMFTQAIQDLNIDPSQSYMVGDKLEDILAAEAAGVKTKVLVRTDKAVT
                     EEGQAKADLVLDSLVDLVKYIK"
     gene            complement(2949..3416)
                     /gene="bcp"
                     /locus_tag="PXD81_000244"
     CDS             complement(2949..3416)
                     /gene="bcp"
                     /locus_tag="PXD81_000244"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HDL5536315.1"
                     /translation="MNILQAGDKAPKFTLQNQADEPVSLSQFAGKKVLVYFYPKALTP
                     GCTTQACGLRDSKSELDELNVVVLGISTDLPKKLAQFVEKKALNFTLLSDPDHQVAEA
                     FGVWGEKKFMGKTYDGIHRISFLIDEQGGVEQVFDKFKTGEHHQMIVDYLRGR"
     gene            complement(3503..3700)
                     /locus_tag="PXD81_000245"
     CDS             complement(3503..3700)
                     /locus_tag="PXD81_000245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5536316.1"
                     /translation="MKFGYLNGVLEGKGKMRFDEAKLHAESEFEKYRIVQDRLFESDF
                     DKVLKEMLRSENQYRPSFSTT"
     gene            complement(3669..4151)
                     /locus_tag="PXD81_000246"
     CDS             complement(3669..4151)
                     /locus_tag="PXD81_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence RhuM family protein"
                     /protein_id="HDL5536317.1"
                     /translation="MIDKQIRNSTAEFLIFTQQNGAENITVRYEDNEIWLTQKLIAQL
                     FDCSTDNVSLHLKNIFNSRELDPNSVTEDFSVTAADVKNYRTKHYNFDAVISIGYRVN
                     SVRATQFRQWATKVLRNFAQKGYVIDRQRMENGTFLNEDYFEQLLAEIREIWLSERRS
                     "
     gene            complement(4294..5451)
                     /gene="hemW"
                     /locus_tag="PXD81_000247"
     CDS             complement(4294..5451)
                     /gene="hemW"
                     /locus_tag="PXD81_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005604493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HDL5536318.1"
                     /translation="MNLILPPLSLYIHIPWCVQKCPYCDFNSHAQKGLIPEQEYIEHL
                     LADLSQDLTAYRVAIGDRKLYSIFIGGGTPSLFSAEGIAYLLIEVEKRIAFEPNIEIT
                     LEANPGTAEAERFLGYAQGGVTRISMGIQSFEPEKLLKLGRIHDANEAKQAIKFAQNS
                     AKSGLQSFNIDLMHGLPNQSVKQALADLETGISLNPPHLSWYQLTIEPNTMFYYRKPT
                     LPDDDELWDIFEQGHQLLTNAGYEQYETSAYAKKGYQCRHNLNYWRFGDYLAIGCGAH
                     GKISYPTGEIYRFSKIKHPKGYMRGEYRYSQDLIELDDRPFEFFMNRFRLLEATPKQE
                     FEWFTGLDREVVRPTIDWALNQNYISETATHWQITQHGKLFLNELLEGFLE"
     gene            5673..7256
                     /locus_tag="PXD81_000248"
     CDS             5673..7256
                     /locus_tag="PXD81_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL5536319.1"
                     /translation="MKKQFEHNESRRGFMKLIAGVGAGMAFSGTLGTFTPKAFAAPAA
                     GSTIEAGIAYPISTGFDPLTSSGASSMAANLHIFEGLVDLHPATRKPYLALAAAEPEK
                     IDDVTYRITLRDGAKFHNGNPVTTEDVVYSFERVLDPAKASLFAQFIPFIDTVKKVDD
                     KVVEFKLKYPFALFKERLTIVKIVPKAVVEAGQAAFDANPVGTGPYKFVSATKDDRIV
                     FEAFADYNGGYPAQVEKMTWFLLSDDAARVTAQESGRVQAIESVPYLDAERLKRKGTV
                     ESVQSFGLLFLMFNCEKAPFNNPKVRQALHYGLDTQKLIDVVFLGNAKAATSYVQDTH
                     PDYVKANSQYDFDKAKAEALLAEAGVTELKFELLATDHSWVKECAPLILESWNSLKGV
                     KVSLKHLQSGALYGTHVDKGAYEVVIAPGDPSVFGNDLDLLLSWWYRGDVWPKRRFRW
                     SETAEYAEVQKLLDEAAKNPAASKEAWAKAINIIAEQVPLYPIIHRKLPTAWNDKALT
                     GFQPLPTTGMSFIGVGRAK"
     gene            7468..8421
                     /locus_tag="PXD81_000249"
     CDS             7468..8421
                     /locus_tag="PXD81_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL5536320.1"
                     /translation="MEIILRLLWRRLMALPVMILGVTALVFIVLQFTPGDPATVALGE
                     SASEAAKEIYREQHGLNDPLLVQYFRFLGNLFVLDFGMTTPPEQPIVDMIAKAFPLTL
                     QLTFIGVFLAAIVSFTLGVTAALYRDTWIDQLIRLISVAAVATPSFWLGILLIQYFSL
                     KLDWLPSGGFIPFSEDPKGYVNSMVLPSLALAVPVCASLIRVVRTTMVEEMDKDYVRT
                     AIGNGVPYSTVIRHNVLRNALITPVTVLGLRVGYLLGGAVVIEQIFDLPGMGKLIFNG
                     IVNHDLNLVQGVVLTIAFTFVLVNIIVDILYLLINPKIRSL"
     gene            8423..10393
                     /locus_tag="PXD81_000250"
     CDS             8423..10393
                     /locus_tag="PXD81_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/oligopeptide/nickel ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HDL5536321.1"
                     /translation="MFRQGLAARLASGGARFRALSTSSKIALLFIVFIALVAIFAPLV
                     APYDPLQTLRPVQAPSGDYLFGTDRLGRDIFSRMVWGARTSLFIGLGAVGVAILFGSI
                     LGATAATADKFGNEVIMRFMDILMAFPGIALAAVLLATFGNSVPVIIVTIAVVYTPQL
                     ARVVRANVVSQWEEDYVRAERVIGGSRTYILLKHVVRNTAAPVLVFATVMVADAIVFE
                     ASLSFLGAGVQPPFPSWGNILSEGRNLVLSGFWWATTFAGIMILLTVLALNILAEGLT
                     DALVNPKLKRSTQSKEDVAKPLATDVQEAMSETLALKRYLLKLHEKETTRTDRMQLNS
                     TAKPILQVKNLSIRFPNRYGETPLVDNISFTVHEGETMGLVGESGCGKSITAFSIMGL
                     LPKTAKITGEILFTDRSGKQHNLLESADLNALRGHEISMIYQDALSALNPSMRIKDQM
                     AQLISRGGKQSAETLLQWVKLDPEKTLNRYPHELSGGQRQRVLIAMALAREPKLLIAD
                     EPTTALDVTVQAEVIKLLNELREKLGFAMVFVSHDLALVAQIAHHITVMYAGQVVETA
                     PTTPLLANPTHEYTRGLLGSVLSTELRAERLYQIPGSVPSPFDFAKGDRFASRSLRPD
                     ANPEQHLKLVPTGDHPQHFWASHLEDPQSKEA"
     gene            10408..11214
                     /locus_tag="PXD81_000251"
     CDS             10408..11214
                     /locus_tag="PXD81_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005623096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL5536322.1"
                     /translation="MSMKFIKEQPIIELSNIVVQFASRDGTLFNPKKFTAVNDVSLAI
                     HAGETVGLVGQSGCGKSTLASVMIGLQKPTSGTVKFNGLEMKYGSAEARKYFGSQVSV
                     IFQDPATALNPRMRVLDILKDPMDIHNILQPQEREKRVYDLLSRVGLPRSAALVEPTR
                     LSGGQKQRVAIARALALNPKLIVADEPTSALDVSVRAQVLNLLADLKKELNLAMVFIS
                     HDLQTVHQVSDRIVVMNGGKIVEMGDASDVFERPTQEYTRTLIEAAPSLL"
     gene            11277..12863
                     /locus_tag="PXD81_000252"
     CDS             11277..12863
                     /locus_tag="PXD81_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HDL5536323.1"
                     /translation="MIQITQTINIDLLIVGSGIAGLSAAVEAKEKGINTLLISKSTIG
                     SGASYFPLKATLGIQVTGDEADKIHFKEDIENIGKGKINPNVVQAYIEDSPQAIELLH
                     RIGFQPWKRNDNRPACFAKYPRPIYLIKDWRESALRAKQILQEQHIAFYENATLLHIV
                     TDNNQVQGAVFSLTTSGQISYIFCKTKHLILAAGGIAGLYKDNLYPADVIGSLHYVAQ
                     QAGATLVNLAFIQFIPAFVEPKYKVLFGEHTLKYVTQITDSKGNNLFPHLNAEQFAKM
                     VLERSHYAPFSLDFAGVEFDLVMMKHLLENPHEKGIYLKYSPKLYEDTEEFYTVYLNW
                     LKNEVGIDLVQDKIAIKPFAHSCNGGIQINQWGESAVKGLFAIGEISHCIEGANRMGG
                     NSVGGGLVFAKRAVSEIVRSLEQNDLQKNSEISPLAMAEKALNQLANSTANLELTASE
                     VLSTIREKMALYANVYRTRSNLKLLLEELNRLEQEFDPIYHAQYQGIEIYNALKTAQL
                     VVNQMLNEQSLGAHYLDEQN"
     gene            12909..14291
                     /gene="nhaC"
                     /locus_tag="PXD81_000253"
     CDS             12909..14291
                     /gene="nhaC"
                     /locus_tag="PXD81_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HDL5536324.1"
                     /translation="MNNYLLKLKPMTAVILSLLTVFILGFTMIGLGWVPQISILAVIV
                     MLLLFGLINKIPFKNMQEGMASGLMSSIGAVYLFFFIGLLVSALMMSGAIPTLMYYGL
                     SVISPELFYISAFALSSVVGIALGSSLTTTATIGVAFIGMASAFGANPAIVAGAVVSG
                     AFFGDKTSPLSDTTTIAASVVGIDMFAHIRNMLYTTIPAWILTALFLWFLTEPTDAQN
                     LANIENLKTNLQETGLIHGYSLLPFVTLILMAIKRVNSIYIIVTTVTLSLIITYIHSS
                     PTLEQLGNYFFTSYKPVEGVDLGEVASLVSRGGINSMFFTITIVLLALSLGGLLQILG
                     ILPALLNGISGFLTSTGRVTLVVASTAVGINVLIGEQYLSLLLSGNTFRSTYERLGLE
                     VKNLSRTIEDAGTVINPLVPWGVCGVFISSVLNVPVLDYLPYAFFCYICLILTLIFGF
                     TGVTISKKAN"
     gene            complement(14334..15530)
                     /gene="ispC"
                     /locus_tag="PXD81_000254"
     CDS             complement(14334..15530)
                     /gene="ispC"
                     /locus_tag="PXD81_000254"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005620038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HDL5536325.1"
                     /translation="MKKLVILGSTGSIGKSTLSVVEHNPNPNQYEVFALVGGKNVELM
                     LEQCRQYQPTFAAMDDEKSAQALKTALATLGSKTEVLAGQAAICELAAHSEVDMVMAA
                     IVGAAGLLPTLSAVKAGKTVLLANKESLVTCGQIFIDEARKSGAKLLPVDSEHNAIFQ
                     SLPPEAQQKVGFCPLAELGVSKIILTGSGGPFRTKPLNEFDAITPAQAVAHPNWSMGK
                     KISVDSATMMNKGLEYIEARWLFNATAEEMDIIIHPQSIIHSMVRYIDGSVIAQMGNP
                     DMCTPIAHTMAYPNRINAGVPPLDFFKLKELTFIEPDFARYPNLKLAIEAFAEGQYAT
                     TAMNAANEIAVEAFLNEQIRFTDIVNVNRTVVENIAPIQVSEIADVLHIDKLARELAK
                     QAVLQL"
     gene            complement(15649..17847)
                     /gene="relA"
                     /locus_tag="PXD81_000255"
     CDS             complement(15649..17847)
                     /gene="relA"
                     /locus_tag="PXD81_000255"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005607154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP diphosphokinase"
                     /protein_id="HDL5536326.1"
                     /translation="MVAIRYSHQLEPNNFELSSWCASLNMSPVTFEQIHNAWSYAQEK
                     LDTDYSLMWYGIEMVEVLHGLNMDDDSLVAAMLFPLVKHNVIDLVQLKEDFSNDIKNL
                     VKGVLEMENIRQLSANNVSDLQIDNIRRMLLAMVDDFRCVVIKLAERIAYLRDSNHHS
                     EEDTVLAAKECSHVYAPLANRLGIGQLKWELEDYCFRILHPVSYTQIAKFELGERRVD
                     RERYIANFVADLTACLSPEIKNVQIYGRPKHIYSIWKKMQKKRLRFNQLFDIRAVRVI
                     VPTLEDCYTALGIIHTKYTHLPEHFDDYIANPKPNGYQSIHTVVLGEGEKTIEVQIRT
                     QQMHDDAELGVAAHWKYKEGATAGRSGYEEKIVWLRKLLEWQNDLADSGDMVAEMRSQ
                     IFDDRVYVFTPRGEVIDLPQKATPLDFAYAIHSEIGHRCIGAKVAGRIVPFTYHLQMG
                     DQVEIITQKEPNPSRDWLNPNTGFVNTGKARSKIIAWFKKLDREKNIPIGKEALEAEM
                     EKFGFTLKQIEEYALPRYNLKQFDDLYAGIGGGDIRLHQLMHFLQSKLIKPTAEQEDE
                     AVLKQVNKNTNQPKPSKGYVIVEGVGNLMHSIARCCQPIPGDEIVGYITQGRGISIHR
                     ADCEQLFELQSLNPERVVEADWGEGYTSGLSLTIRVIANDRNGLLRDVSAIMANEKVN
                     VLGVSSRTDVKRSLATIDMEIQLNNVEILNKLLARIAQLDDVIEAKRLSS"
     gene            18101..19408
                     /locus_tag="PXD81_000256"
     CDS             18101..19408
                     /locus_tag="PXD81_000256"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009875152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HDL5536327.1"
                     /translation="MTILALGINHKTASVGLREKVAFVDDKRKLALEQIQTSGLAESV
                     VILSTCNRTELYFHQPNISPREESEENIQWREQCFRWFAQIHQLDESELRECLYFKQN
                     LEAANHLMKVASGLDSLILGEPQILGQVKQAYQYSEDFYQHQQKAMSTKLSRLFQRTF
                     ATAKRVRSETEIGSQAVSVAYAACGLARQIFDNFGKLRFLLVGAGETIELVARYLIQH
                     GAKNIMLANRTHIRAEMLAEKLNAPMQILSLSALQLGLNQADVVISSTGSPDTLISKE
                     MVEIAQKQRQFDPLLLIDIAVPRDIDEKAGDLEGVYAYSVDDLQNIIQRNMAQREEAA
                     KQAAEIVKEESKAFFEWLKQQQSSDLIKRYRQDAEQTRQELLEKALQAISQGQDSQKV
                     LSELSYKLTNQLLHAPTQALQAMAKSGNSTGLQSFSRALRLEE"
     gene            19593..21128
                     /gene="der"
                     /locus_tag="PXD81_000257"
     CDS             19593..21128
                     /gene="der"
                     /locus_tag="PXD81_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005614634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HDL5536328.1"
                     /translation="MTPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGH
                     ANIAGYDFIVIDTGGIDGTEEGVEEKMAEQSLLAIEEADVVLFLVDARAGLLPADVGI
                     AQYLRQREKTTVVVANKTDGIDADSHCAEFYQLGLGEVEQIAAAQGRGVTQLIEQVLA
                     PLGEQLNAEQFNQEQAGENAKNVANEETDEWDTEFDFENEEDAALLDEALAEEAEESI
                     EDKNIKIAIVGRPNVGKSTLTNRILGEERVVVYDMPGTTRDSIYIPMERDGQQYTIID
                     TAGVRKRGKVNLAVEKFSVIKTLQAIQDANVVLLTIEAREGISDQDLSLLGFILNAGR
                     SLVIVVNKWDGLSQDIKDQVKSELDRRLDFIDFARVHFISALHGSGVGNLFDSVKEAY
                     ACATQKTSTSMLTRILRMAADEHQPPLVNGRRVKLKYAHPGGYNPPIIVIHGNQVEKL
                     ADSYKRYLSNYFRKSLKIIGSPIRILFQEGNNPFAGKKNKLTPSQLRKRKRLMKFIKK
                     SKK"
     gene            21224..22651
                     /gene="hldE"
                     /locus_tag="PXD81_000258"
     CDS             21224..22651
                     /gene="hldE"
                     /locus_tag="PXD81_000258"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HDL5536329.1"
                     /translation="MMMQYSPKFNNAKVLVLGDVMLDRYWFGATNRISPEAPVPVVKV
                     QDIEERAGGAANVAMNIASLGVPVTLHGLIGDDDAGRALDKLLNSHQIQNHCVVINSH
                     PTITKLRILSRHQQLLRLDFEEGFHNVDSTALLAKLSQEITAYGALILSDYGKGTLES
                     VQQMIQIARNAKVPVLIDPKGTDFERYRGATLLTPNMSEFEAVVGHCKDEDEIVSKGL
                     KLIADYDLSALLVTRSEKGMTLLRPNHEPYHLPTQAKEVYDVTGAGDTVISVLATAIA
                     DGRAYEEACYLANAAAGVVVGKLGTSTVSPVELENAIHQRTATGFGVVSEAELKAIVE
                     EAKKRGEKIVMTNGCFDILHPGHVSYLENARKLGDRLIVAVNTDASVKRLKGEARPIN
                     DLAARMAVLAGLASVDWVVPFDEDTPQRLISEILPDLLVKGGDYKPEEIAGSQEVWAN
                     GGDVRVLNFENGCSTTNVIKKIQAL"
     gene            22665..23219
                     /gene="pyrR"
                     /locus_tag="PXD81_000259"
     CDS             22665..23219
                     /gene="pyrR"
                     /locus_tag="PXD81_000259"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HDL5536330.1"
                     /translation="MAREKIIIDTEQFQRTISRISHQIIEKHGDLQDVVIVGIKRRGA
                     EIAELIQKRIEDLAQTRLPYMALDITFYRDDLDLIYQDPVFMGADNQLNLNGKKVILV
                     DDVLFTGRTIRAALDALLDFGRAARIELVIFVDRGHRELPIRADYVGKNIPTAKNEQI
                     QVQTLSYDGINQVVLVPAANKESA"
     gene            23292..24242
                     /locus_tag="PXD81_000260"
     CDS             23292..24242
                     /locus_tag="PXD81_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007523149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDL5536331.1"
                     /translation="MKMKLVKSLFAVAVATMSLTQTAHAFEERVVALVNDTPIMQSQV
                     QRVLGKKKATETAQRAAIDQVIDDMLVQQAMKEAGVKVSPAAVNQAVENVAIQNGITY
                     GQLLDALDYQGITLEQFKRNIAQQMAMEQVRHISISKSIQVAPQQVQALAKELMAKDK
                     AAGKLKTVSGKEHRISHILLKTNPILNDAQAKAKLSSLTTDINSGKISFEEAAKTHSV
                     DYASGADGGDLGFNFLEIYDPAFANVASKIKPNQISAPFKSQFGWHILKVTDTRNGDR
                     TEDAYHQRAYQQLVNKQAEEASKDWVKALRKTANIQYVGQ"
     gene            24304..25170
                     /gene="rsmA"
                     /locus_tag="PXD81_000261"
     CDS             24304..25170
                     /gene="rsmA"
                     /locus_tag="PXD81_000261"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HDL5536332.1"
                     /translation="MSSTNSKKHLGHTARKRFGQNFLHDMNIIHSIVSAINPKNGQFL
                     LEIGPGLGALTEPVAELVDKLTVVEIDRDLAERLRHHPFLNQKLTIIEQDALRFNFRD
                     YFNQLNLDEDSVRVFGNLPYNISTPLIFHLLTFHDLIQDMHFMLQKEVVKRLCAAPNS
                     KAYGRLTIMAQYYCQVIPVLEVPPTAFKPAPKVDSAVVRLVPYNQLPYPAKDVYWLNR
                     VTTQAFNQRRKTLRNALSTLFSAEQLDALGVDLTARAENLTIADYVRLANWLHDNPPA
                     IDTTEELVDEDC"
     gene            complement(25281..25910)
                     /gene="upp"
                     /locus_tag="PXD81_000262"
     CDS             complement(25281..25910)
                     /gene="upp"
                     /locus_tag="PXD81_000262"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HDL5536333.1"
                     /translation="MKIVEVKHPLVKHKLGLMREAEISTKDFRELANEIGSLLTYEAT
                     KDLEMESVEINGWSGEKIAVERIKGKKVTVVPILRAGLGMMDGVLEHIPSARISVVGI
                     YRDEETLKPVPYFSKLANDVDERLAIITDPMLATGGSMVATIDLLKKAGCKQIKVLVL
                     VAAPEGIKVLEAAHPDVELYTASIDSHLNENGYIIPGLGDAGDKIFGTK"
     gene            complement(25935..27620)
                     /gene="fadD"
                     /locus_tag="PXD81_000263"
     CDS             complement(25935..27620)
                     /gene="fadD"
                     /locus_tag="PXD81_000263"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HDL5536334.1"
                     /translation="MEKIWLNNYPPYSPKTIETDQYESLVEMFEAAIQRHPDIPAYIN
                     MGQVLTFRKLEERSRSFAAYLQNELRLKKGDRIALMMPNLLQYPIALFGALRAGLVVV
                     NVNPLYTPRELEHQLNDSGAKAIVIVSNFAATLEKIVFNTEIKHVILTRMGDQLSFGK
                     RTLVNFVVKYIKKLVPKYKLPHAVSFREALSIGKQRQYVKPNIVSQDLAFLQYTGGTT
                     GVAKGAMLTHQNLIANMMQAKWIVEPLLGNSANMIGLVPLPLYHVLALSMNCLLFIEL
                     GLTGLLITNPRDIPGFIKELKKYKVSVITGVNTLFNALLNHESFKEVDFSHLKLSVGG
                     GAAIQSSVAKRWHDLTGVHIIEGYGMTECSPLISATRGDSVEYSGSIGVPVPNTDIRI
                     VDDEGKEVPLGERGELVVKGPQVMQGYWNRPDETAQVLQDGWMATGDIVVMGDDLNLR
                     IVDRKKDMIIVSGFNVYPTEIEEVISQNPKVNEVVAIGVPSEASGESIKIFVTKKDES
                     LTRDELRTYCRQFLTGYKIPRDIEFRDELPKSNVGKILRRVLRDEELAKVNKA"
     gene            complement(27598..28062)
                     /locus_tag="PXD81_000264"
     CDS             complement(27598..28062)
                     /locus_tag="PXD81_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR reducing system RseC family protein"
                     /protein_id="HDL5536335.1"
                     /translation="MMIEQATVVEYQNGVAIVQCYAKSSCGGCVAQSACGSKALSALA
                     GEKIDPRFRIEVDEPLKVGDKVELGLAENILLKSVFLIYGIPLLVLVMTAVGFSQFFA
                     NELIVLFIMLLSTGITFWAVRKLIHKHSKQANFSPIFLGKVLGDYNGKNMAK"
     gene            complement(28059..>28896)
                     /locus_tag="PXD81_000265"
     CDS             complement(28059..>28896)
                     /locus_tag="PXD81_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MucB/RseB C-terminal domain-containing protein"
                     /protein_id="HDL5536336.1"
                     /translation="VYILQQGSDTESFRLRHAFTHNKEYAQLLNLDHSREEIILKNNK
                     VSYLGYNFRPFSLNSSQILDNLPNVLYTDYANLKGYAFLDAGKDRISDRITKVIRMKP
                     NDTLRYAYILWIDEETHLLLKSQVLNMDNLVLEEFRVLQLYQSPELDRIANVIESLML
                     PTLTTVKKEEFPKSDGWQVKWLPTGFRLIQQQTVTGATYQVESEYVDSRVYSDGLSTL
                     TIYIMPSQGVSFNEYAWQQGKLTILNQTINDRDIVIIGDVPLQSAKQIMNNIGFKEGA
                     QP"
BASE COUNT         8346 a         6246 c         5776 g         8528 t
ORIGIN      
        1 acttcaaata atgccttggt tttatgcact ccaatggctt tcaaccatca ctgcatttct
       61 ctatttagtg atttttgcca ctattaccgt gtatgcgaat acggcactat ctaatatgct
      121 agtgaaatta gacaatggta aaaaccaagg cttaatgttt ggaagctcta aattaatttt
      181 agctattacc actctcggag taatgaatgt attaccttac attttcttaa tctaagcagg
      241 gtaaatcacg cccaaactca cggctttttt tgctcctgtc acgctaggca tattactggg
      301 taaattatgg attcgctggt aagctaacca agcaaaagcg gcagcctcta cataatcaat
      361 atctaaacca aattctgtag tggttgtaac ctcccactcg ggaagtaaag cagaaatccg
      421 ttccattaat aacggatttc ttgacccacc accacaaact aaaagcaagc gaggcagaga
      481 gctttcaaag cgtaataact cattggcaat actttgagca gtaaattctg ccagcgtgcg
      541 ttgcacatct tgtggctgac aagcggtcaa attttccaat ttttttgcaa gccacgttaa
      601 attaaacagc tcccgcccag tgcttttagg cggggcttta gcaaaaaacg actcgtctaa
      661 taacgtcgct aacagttcag tattgagctt gcccgttctc gcccattcgc cgtttttatc
      721 gtatcttttg ccttgatgtt gttcgatcca actatccatc aaagtatttc cggctcctac
      781 atcataacca actgtagggg aattgggatt taacaccgaa atattgctga tgccgccaat
      841 attcagcacc actgttagac gatttggatc gctaaaaata gcttgatgaa atgccggcac
      901 aagcggagcc ccttgcccac ctactgccat atctttacgc cgaaaatcag cgatagtggt
      961 aataccggtt tgctctgcca ccaaattcat atcgccaatt tgggtggtga agggatactc
     1021 agaatccggt gaatgccaca ccgtctgccc gtgacaacct atggcttgaa tatcttctgc
     1081 ttttagctga tttttcgcta aaaacgcatt gatcgtttct gcataaagca agcctaaacg
     1141 gtgatcaatt tcccccagtt tttggagatt ggtttcgccg gttttaagta acgcagaaat
     1201 atccgcccgt aattcctccg gcattggaaa aaaatcacaa gcggtcattt tcggtggatt
     1261 ttttgcaaaa tccagcaaag caatatccac cccatcaagg ctcgtgccgg acattacgcc
     1321 tatatagtaa tttgggctat attcattcat cataaaaatc aacatctgct atttgttact
     1381 tttctttgct ttgccaaaga aaagtaacca aaagaaaggc aacccgagct tcaccgctat
     1441 cctcgcttag tttgaattgg cttaacggca aatttacatt cgctccgctc agagtaaatt
     1501 tacctaccaa ttcaaacacg ctcaggcggt tcagacggga acaaaaaaca acaaaatcat
     1561 tggcgtgcca ttcttagatt ttccggcact tcctcaaaat tcgatctaaa cggattgatg
     1621 tctagcccgc ctcgtctggt gtagcgtgcg taaactgtta atttttccgg ctcggcaaat
     1681 tgcattaaat cacagaaaat gcgttccaca cattgttcgt ggaactcatt atgctcacgg
     1741 aacgacacta aataacgtaa cagtttttca cggtttagct ttttgccttt gtagcgaatt
     1801 tgcacacttc cccagtcagg ctgtgacgta attaagcagt tagatttgag caaatggctg
     1861 actaaggttt cttccaccaa ctcatcttct gcaatgttct ctaaataatg agtcgaaaac
     1921 gcataactgt cgatttgaat atcttgctca tcaatgcaat cgcctgcaaa atcaactatc
     1981 ggctgttgcg tgtagtgtga taattggtaa atttttaccg aaacctgacc gcttgcacag
     2041 cgagccagat ccgcttgaat cgttttccca acttcttcta ttgagctaaa tttggtttgg
     2101 ttaaaactgt tcaaatagag cttgaaactt ttcgattcaa ttaagttttc gctcttaaaa
     2161 tcaatggcga catctgcaat cgctacttgc ggtagcccat tttggttcaa ccaagatagc
     2221 tcatagcaag tccacacatc agcccctcgg ttaaaaggct gctcttgtgt aatgcctaaa
     2281 ctatcacggt ttaatttacg aggaacaggt tgcaaaagcg aggcatcata ttcgcttttg
     2341 tactcggttt tttgccctaa tttcagggcg gaaagggatt tatcgttata attcattgct
     2401 ttatttaata tattttacta aatcaactaa actatctaac accaaatcgg ctttagcctg
     2461 accctcttcc gtcactgctt tgtcagtgcg aaccagcact ttggttttca cgccggcggc
     2521 ttctgcggca aggatatctt ctaatttatc gcccaccata taagattggc ttgggtcgat
     2581 gtttaaatct tgaatcgctt gagtaaacat acctgcttta ggcttacggc aatcacaatc
     2641 tttacggtat tcgcctttgc cttcggggtg atgtgggcag tagtaaatac cgtctagtac
     2701 cacgccataa tcttcatcga gcgaccagtc catccattcg gttagttgca taaattgtgc
     2761 ttcgctaaaa tagcctcggg caatgccaga ttgattggtt accagcacca ataaataacc
     2821 tttgtcttgt agctgtttta acgccttgcc gacaccgtca ataaattgaa aatcatcaat
     2881 ttgatgcaca tagccgtgat cgatattaat tgtgccgtca cggtctaaaa aaatggcttt
     2941 ttttcccatt agcgaccccg tagataatcc acgatcattt ggtgatgttc gccggtttta
     3001 aatttatcaa acacttgctc aacccccccc tgctcatcaa ttaaaaaact gatgcgatga
     3061 atgccgtcat aggtttttcc cataaatttc ttttcgcccc atacaccaaa ggcttctgca
     3121 acttggtgat ccgggtcaga taataaggta aaattaagtg cttttttctc tacaaattga
     3181 gctaattttt tcggtaaatc ggtgctaatt ccgagaacaa ccacattgag ctcatcaagt
     3241 tctgatttgc tgtcacgcag tccgcacgct tgcgtagtgc aaccgggtgt aagagctttc
     3301 ggataaaaat aaaccagtac ttttttgccg gcaaattgag agagagaaac cggctcgtca
     3361 gcctgattct gaagggtaaa tttaggggct ttatcgcctg cttgtaagat attcatttgt
     3421 aatttttcca taaaaaataa ccgcttgcat tatacacggc aagcggtcaa acttggcaaa
     3481 aaatttgcaa aatgcggttg ttttatgttg ttgaaaatga tggacggtat tgattttccg
     3541 aacgcaacat ttctttcaat accttgtcaa aatcgctttc aaataatctg tcttgaacaa
     3601 tgcggtattt ctcaaattcg ctttctgcgt gcaatttcgc ttcatcaaaa cgcattttgc
     3661 ctttgccctc aagaacgccg ttcagataac caaatttcac gaatttctgc cagcaactgc
     3721 tcaaaataat cttcatttaa aaatgtacca ttttccattc tttggcggtc aatcacatag
     3781 cctttttggg cgaaattccg taatacttta gttgcccatt ggcgaaattg tgtcgcccgc
     3841 accgagttta ccctatagcc tatcgaaata acggcatcaa agttgtagtg tttggtacga
     3901 taatttttga catctgcggc agttaccgag aaatcctcgg taactgaatt tggatcgagt
     3961 tccctactat taaaaatgtt ttttaaatgc aaagaaacat tatctgttga gcaatcaaaa
     4021 agctgagcaa ttaatttttg cgtaagccaa atttcgttat cttcatagcg aacggtaata
     4081 ttttctgccc cattttgttg agtaaaaatc aaaaattcgg cggtactatt acggatttgt
     4141 ttatcgatca taagctctcc ttattgtgaa ggctatgcta ctaaatactg tttttttgtc
     4201 tagtaaaaaa tgaggatttt tcgacgcaga tcacaaaaaa cgcctgtaat ttagcattac
     4261 aatgcggtct ttttttagtc gtttttttgc aaattattcc aaaaatcctt ccagtaattc
     4321 atttaaaaac aatttaccgt gttgggtaat ttgccaatgg gttgcggttt cggaaatata
     4381 gttttggttt aacgcccaat cgattgtcgg gcgaacaact tccctgtcta accctgtaaa
     4441 ccattcaaat tcttgttttg gtgtcgcttc aagtaatcta aagcggttca taaaaaactc
     4501 aaacgggcgg tcgtctaatt caatcaaatc ttggctgtag cgatattcac ctcgcatata
     4561 acctttcggg tgtttgattt tgctaaagcg ataaatttcc ccggtcggat atgaaatttt
     4621 gccgtgagcg ccacaaccaa tcgccaaata atccccaaat cgccaataat ttaaattgtg
     4681 tctgcattgg tagccttttt tggcataagc cgaggtttca tactgctcat agccggcatt
     4741 cgttaaaagt tgatgccctt gctcgaaaat atcccataac tcgtcatcat caggcaaggt
     4801 tggcttacga taatagaaca tcgtattcgg ctcaatcgtg agctgatacc acgataaatg
     4861 cggtggattg agtgaaatcc ctgtttctaa atccgccaag gcttgtttaa ccgattggtt
     4921 tggtaagccg tgcattaaat caatgttgaa actttgcaag cccgattttg cagaattttg
     4981 ggcaaattta atcgcttgtt ttgcttcatt tgcatcgtga atccgtccta atttcaataa
     5041 tttttccggc tcaaaacttt ggattcccat tgaaatgcgg gttacgccac cttgtgcgta
     5101 acctaaaaat cgctctgcct ctgcggttcc cgggttcgct tccagtgtaa tttcgatatt
     5161 tggctcgaat gcaatccgtt tttctacttc aattaataaa taagctatcc cttccgctga
     5221 aaataagctc ggggtgccgc cgccaataaa aatagaatat aatttacgat cacctattgc
     5281 cactcggtaa gcggtcaaat cttgggataa atctgcaagt aaatgttcga tatattcttg
     5341 ctcgggaatt aatccttttt gagcgtgcga gttaaaatcg caataagggc atttttgcac
     5401 gcaccaaggg atatggatat aaaggcttaa aggcggtaaa atcaggttca ctgtcaattt
     5461 ttcatttatg attaaattgg cgaataataa cgaaaagcac gtgaagtgaa aagcaaagat
     5521 tttcgtgagc tagatcacaa attcaataat tcttgcttta ccactttgac attaatgata
     5581 tgataaattc aattcaaaaa agtgcatttt ttgataaatt cacttcagca aataataccg
     5641 cccctatcat aaaaacttaa aggagcttta agatgaaaaa acagtttgaa cacaacgaat
     5701 cccgccgtgg ttttatgaaa ctaattgcag gtgtcggtgc cggtatggca tttagtggca
     5761 ctttaggtac atttacacct aaagcatttg cagcccctgc cgccggctca acgattgagg
     5821 ccggtatagc ttatcccata tcaaccggat ttgacccgct tacctcaagc ggtgcatcct
     5881 caatggcggc aaacctccat atttttgaag gcctcgtgga cttacatcca gccactcgca
     5941 aaccatattt agcattggca gcagcagagc ctgaaaaaat tgatgatgta acctatcgaa
     6001 ttactttacg tgacggggct aaattccaca atggtaatcc ggttaccact gaagacgtag
     6061 tttactcatt tgaacgtgta ttagacccag ctaaagcatc acttttcgca caattcattc
     6121 catttattga cactgtgaaa aaagttgatg acaaagtggt tgaattcaaa ttgaaatatc
     6181 catttgcgtt attcaaagag cgcctaacca ttgttaaaat cgtaccaaaa gcagtggtag
     6241 aagccggtca agctgcattt gatgctaatc cagtggggac tggcccatat aaatttgtat
     6301 ccgcaactaa agatgatcgt attgttttcg aagcatttgc agattataac ggtggctacc
     6361 cggcacaagt cgagaaaatg acttggttct tactttctga tgatgccgct cgtgtaaccg
     6421 ctcaagaatc tggccgtgtt caagcaattg agtctgtgcc ttacttagat gcagaacgct
     6481 taaaacgtaa aggtaccgtt gagtcagtac aatcattcgg cttactgttc ttaatgttta
     6541 actgtgaaaa agcaccgttt aacaacccga aagttcgcca agcgttacat tatggcttag
     6601 atacccaaaa attaattgat gtggtgttct taggcaatgc aaaagctgct acatcttacg
     6661 tgcaagatac tcacccggat tatgtgaaag caaattcgca atatgatttc gataaagcga
     6721 aagccgaagc gttattagca gaagcgggtg tgacagagtt aaaatttgaa ttgttggcaa
     6781 ccgatcactc ttgggtgaaa gaatgtgcgc cattaattct tgaatcgtgg aacagcttaa
     6841 aaggcgtgaa agtttcactc aaacacttac aatcaggtgc attatatggc acacacgttg
     6901 ataaaggtgc atacgaagtt gtgattgcac ctggcgaccc atcagttttc ggtaatgact
     6961 tagacctatt attaagctgg tggtatcgtg gtgatgtatg gccgaaacgt cgtttccgct
     7021 ggtctgaaac agctgaatat gcagaagtac aaaaactgct tgatgaagcg gccaaaaatc
     7081 ctgctgcttc taaagaagcc tgggcgaaag caatcaatat tatcgctgaa caagtgcctc
     7141 tttacccaat tattcaccgt aaattgccaa ctgcgtggaa tgataaggca ttaacaggtt
     7201 tccaaccatt accaacaaca ggtatgtcat ttatcggtgt tggtcgtgct aaatagtcgt
     7261 aacggctagc taaatgcaag cggtaatatt ttacaaaaaa tttaccgctt gcataaataa
     7321 aaatcaaaat gatgtaatat tcgtcttata tcactcagaa ttcttaccat ttctatattt
     7381 tcatttataa tcctacatat cactatatta gcttgtaggt aaacttagat ttaattttgg
     7441 agtgtaacta cactcaagga gaagaaaatg gaaattatac tccgattatt atggcgtcgc
     7501 ctaatggctc tgccggtaat gatcttaggc gtaactgcct tggtatttat tgttttacaa
     7561 ttcacccccg gagatccggc aaccgttgct ctcggtgaaa gcgccagcga agccgctaaa
     7621 gaaatctatc gtgaacaaca tgggttaaat gatccattgc ttgtacaata ttttcgcttt
     7681 ttagggaatt tattcgtatt agatttcggt atgacaaccc ctccagaaca gcctattgtt
     7741 gatatgattg ctaaagcatt ccctttaaca ttacagctta cctttattgg ggtcttctta
     7801 gctgctatcg tctctttcac attaggtgta acggcagcac tttaccgtga tacttggatt
     7861 gatcaattaa tccgtttaat ctcggttgcc gctgttgcca caccttcttt ctggttgggt
     7921 attttattga ttcaatactt ctccttaaaa ttagattggt tgccttcagg cggttttatt
     7981 ccatttagcg aagatccaaa aggctatgta aactcaatgg ttctcccttc actcgcttta
     8041 gctgttcctg tatgtgcctc attgatccgt gtcgtgcgta caacaatggt agaagaaatg
     8101 gataaagatt atgttcgaac agccatcggt aatggtgtac cttactcaac ggtgattcgc
     8161 cacaacgtgc ttcgtaatgc cttaattact ccggtaacag tattaggttt acgggttggt
     8221 tatttattag gtggtgcagt tgtgattgaa cagatcttcg acttaccggg aatgggtaaa
     8281 ttaattttca acggtatcgt caatcacgac ttgaacttag tgcaaggtgt agtattaact
     8341 atcgcattta ctttcgtgtt ggtgaatatc attgttgata ttctttactt gctcattaat
     8401 ccaaaaattc ggagtttata atatgtttcg ccaaggatta gctgctcgac ttgcttcagg
     8461 cggtgcaaga tttagagcat tatcaaccag ctcaaaaatc gccttactct ttattgtatt
     8521 tatcgcatta gtcgctattt ttgcaccatt agtagcgcct tatgacccac tgcaaacgct
     8581 acgtccagtg caagctccaa gcggtgatta tttattcggt acagaccgtt taggacgtga
     8641 tattttctcg cgtatggtat ggggagcaag aacatctctc tttatcggtt taggtgcggt
     8701 aggcgttgcg attctattcg gtagtatttt aggggcaact gcggcaaccg ccgataaatt
     8761 cggtaacgaa gtgattatgc gttttatgga tatcttaatg gcattcccgg gtatcgcatt
     8821 agctgctgta ttacttgcca ctttcggtaa ctctgtacct gtgattatcg tgactattgc
     8881 ggttgtttac actccccaac ttgcccgagt ggtgcgtgct aacgtagtat ctcaatggga
     8941 agaagattat gtgcgtgccg agcgtgtaat tggtggtagc cgtacttata ttctattaaa
     9001 acacgttgtg cgtaatactg ctgcaccggt tttagtattc gcaacagtga tggttgctga
     9061 tgccatcgta tttgaagcat cactttcatt cttaggtgca ggtgtccagc ctccattccc
     9121 atcttgggga aatatcttat ctgaaggtcg taaccttgta ttaagtggct tctggtgggc
     9181 tacaactttc gcagggatta tgattttatt aaccgtactt gcgttaaata tccttgctga
     9241 aggcttaacc gatgcgttag taaatccaaa actaaaacgt tcaactcaaa gtaaagaaga
     9301 tgtcgcaaaa ccattggcaa cagacgttca agaagcaatg tctgaaactc ttgcgttaaa
     9361 acgttactta ctcaaattac acgagaaaga aacgacccgt actgaccgta tgcaattaaa
     9421 ctcaacggcc aaaccgattt tacaagtgaa aaacttatca atccgtttcc caaatcgtta
     9481 tggtgaaact ccgcttgttg ataatatcag ctttactgtt cacgaaggcg aaaccatggg
     9541 cttagtgggt gaatctggtt gtggtaaatc aatcaccgca ttctcaatta tgggtttatt
     9601 accaaaaact gcaaaaatca ctggtgaaat cctctttacc gaccgttcag gtaaacaaca
     9661 caatctttta gaatcagccg atttaaatgc ccttcgtggt cacgaaatct cgatgattta
     9721 ccaagatgca ttaagtgcgt taaacccatc aatgcgtatt aaagaccaaa tggcacaatt
     9781 aatcagccgt ggtggtaaac aatcagcaga aacattattg caatgggtaa aacttgaccc
     9841 ggaaaaaacg ctcaaccgtt atcctcacga gttatctgga ggacaacgtc agcgtgtatt
     9901 aattgcaatg gcacttgctc gtgaaccgaa actattgatt gctgatgaac caacaacagc
     9961 attagacgta accgttcaag ctgaagtgat caaattatta aacgaattac gtgagaaact
    10021 tggatttgca atggtattcg tgagccacga cttagcttta gtggcacaaa tcgctcacca
    10081 catcacggta atgtatgctg gtcaagtggt tgaaactgca ccaactacgc cattattggc
    10141 aaacccaaca cacgaataca cacgtggatt attaggctcg gtactttcta ccgaattgcg
    10201 tgcagaacgc ctataccaaa ttccgggcag tgtgccttca ccatttgatt ttgcgaaagg
    10261 tgaccgtttt gcgagtcgtt cattaagacc agatgcaaac cctgaacagc acttgaaatt
    10321 agtgccaacc ggtgatcacc cacaacactt ctgggcttca cacttagaag atccacaatc
    10381 taaagaagca taaaggagag caatacgatg agtatgaaat ttatcaaaga acagccaatt
    10441 attgaactgt ctaatattgt tgtgcaattt gcttctcgtg acggcacctt atttaacccg
    10501 aaaaaattca cggcggtaaa tgatgtcagc ctagcaattc acgccggtga aacagtcggt
    10561 ctggttggac aatctggttg tggcaaatct actttagcca gtgtgatgat cgggcttcaa
    10621 aaaccaacct ccggcacagt aaaatttaac ggcttagaga tgaaatatgg cagtgcggaa
    10681 gctcgcaaat atttcggtag ccaagtgtca gtaattttcc aagacccggc aaccgcccta
    10741 aatccgcgta tgcgtgtgtt ggatatttta aaagatccga tggatattca taatatctta
    10801 caaccgcaag aacgtgagaa gcgggtttat gatttgctct ctcgagtcgg tctacctcgt
    10861 tcagcggcgt tggtcgagcc aacccgatta tctggtgggc aaaagcaacg tgtagcgatt
    10921 gctcgtgcat tggcattaaa tccgaagctg attgtggctg atgaaccaac ctctgcactt
    10981 gacgtatcgg ttcgagctca agtactgaac ctgctggcag acttgaaaaa agaactcaat
    11041 ttagcgatgg tatttatctc tcacgactta caaaccgttc accaagtttc agaccgcatt
    11101 gtggttatga acggcggtaa aattgtggaa atgggcgatg cttcagacgt atttgaacgc
    11161 ccaacgcaag agtacacccg aaccttaatt gaagcagcac catctttatt ataattgtgc
    11221 tttgtgccta caagcggtaa aatttttcgc aaattttacc gcttgtgttt ttatttatga
    11281 tccaaattac ccaaaccatt aacattgatt tattaattgt cggctctggc atcgctgggc
    11341 tttctgctgc agttgaggca aaagaaaaag gcattaatac cttactgatt agcaaatcta
    11401 cgattggctc gggagcgagc tactttccgc ttaaggcaac acttggcatt caagtaaccg
    11461 gcgatgaagc cgataaaatt cactttaaag aagatattga aaacatcggc aaaggcaaaa
    11521 tcaaccctaa tgtggtacaa gcctatatcg aagattcacc acaagcgatt gaactactcc
    11581 accgtatcgg ctttcagcct tggaagcgta acgacaaccg tccggcttgt tttgccaaat
    11641 accctcgccc aatttattta attaaagatt ggcgggaatc tgccctacga gcgaaacaga
    11701 ttttgcaaga gcaacacatt gctttctatg aaaatgctac ccttttacac atcgtcacag
    11761 acaataacca agtgcaaggt gcggtatttt cgctcactac aagcggtcaa atttcctata
    11821 ttttttgcaa aaccaagcat cttattttag ccgcaggcgg gattgctggg ctgtataaag
    11881 ataatctcta cccggctgat gttatcggtt cacttcatta tgtggcacaa caagccgggg
    11941 caaccttggt taatcttgcc tttattcagt tcattcctgc ctttgtcgag ccaaaataca
    12001 aggtgctatt tggcgaacat acattgaaat atgtcaccca aatcaccgat tctaagggta
    12061 acaatctatt tccacactta aatgccgagc aatttgcaaa aatggtgctg gaacgtagcc
    12121 attatgctcc tttcagcctt gattttgccg gcgtggagtt cgatttagtg atgatgaaac
    12181 atttgcttga aaaccctcac gaaaaaggca tttaccttaa atacagcccc aaactgtatg
    12241 aagacacgga agagttttat accgtctatt tgaattggct caaaaatgaa gtcgggattg
    12301 atttagtgca agataagatt gcaatcaagc cgtttgctca tagctgtaac ggcggcattc
    12361 aaattaatca atggggcgaa agtgcagtaa aagggctatt tgcgattggt gagatttctc
    12421 actgcattga aggggcaaac cgtatgggtg gtaactcagt cggcggtggc ttagtgtttg
    12481 ccaaacgtgc ggtatcggaa attgttcgtt cgctagaaca gaatgatttg caaaaaaaca
    12541 gcgaaatctc accgcttgca atggcagaaa aagcattaaa tcagcttgct aattcaactg
    12601 ctaatctaga actaactgcc agtgaagtgc tttctactat tcgagagaaa atggcgttat
    12661 acgccaatgt gtaccgcact cggagcaacc tcaaactact acttgaggaa ttaaaccgtt
    12721 tggaacaaga atttgaccca atctatcacg ctcaatatca aggcattgaa atttacaatg
    12781 cgttaaaaac cgctcaatta gtggtcaatc aaatgctaaa tgagcagagt ttaggcgcgc
    12841 actacttaga tgaacaaaat tagtctataa tagccaactt atttaaaaat aataaattag
    12901 gagtataaat gaataactac ctacttaaat taaaaccaat gacagcggtc atactttcgc
    12961 ttttgacggt ctttatctta ggttttacaa tgattgggct tggctgggtg cctcaaattt
    13021 caattctagc cgtgattgta atgctgctac ttttcggctt aatcaataaa attccgttta
    13081 aaaatatgca agagggtatg gcaagcgggc tgatgtccag tattggtgcg gtttacctat
    13141 tcttttttat tggattattg gtttcagctt tgatgatgtc tggtgcgatt ccaaccttaa
    13201 tgtattatgg cttaagtgtg atttcacctg aattatttta catttctgca ttcgcattaa
    13261 gttctgtagt aggtattgcg ttaggcagta gtttaaccac aaccgccacc atcggtgttg
    13321 cttttatcgg tatggcttct gctttcggtg cgaaccctgc gattgtagca ggtgcggtgg
    13381 tttcaggggc gttttttggc gataaaacct ctccactttc agacactact accattgctg
    13441 cttccgtagt gggcattgat atgttcgctc atatccgtaa tatgctttat accaccatcc
    13501 ctgcgtggat tttaacagca ttattccttt ggtttttaac cgaacctact gatgcacaaa
    13561 atttagcaaa tatagagaac ctaaaaacca atttgcaaga aacaggatta atacacggct
    13621 actctttgct accttttgtt acgttaattt taatggcaat taagcgtgtt aattcaattt
    13681 atattattgt aactacagta acactctcgc taattattac ctatatccac agttcgccaa
    13741 cccttgaaca gctagggaat tatttcttta ccagctataa acctgttgaa ggcgtagatt
    13801 taggcgaagt ggcatcatta gtctctcgtg gtggcattaa tagtatgttc tttaccatca
    13861 ctattgtatt gcttgcgtta agtttaggtg gtcttttaca aatcctcggc attttaccgg
    13921 cactacttaa cggcattagc ggctttttaa ccagcacagg acgtgttact cttgtagttg
    13981 ccagcactgc ggtgggaatt aacgtcttaa tcggtgagca atatttgagc ttattgttat
    14041 caggtaatac tttccgctca acttatgaac gtttaggctt agaagtgaaa aacttatctc
    14101 gcaccattga agatgcaggc acggtaatca acccattagt cccttggggc gtgtgcggtg
    14161 tgtttatcag tagcgtatta aatgtaccgg tgttagatta tctaccatac gcattcttct
    14221 gttatatctg tttaatcctt accttaattt ttggatttac cggggtaaca atcagtaaaa
    14281 aagctaacta atatgtgaaa aatgcctaca aatttgtagg cattttcttt tcattagagc
    14341 tgaagaacag cttgtttggc taactctctg gcgagtttat caatatgcaa tacatcagca
    14401 atttcactga cttgaattgg agcaatattt tccaccaccg ttctattcac gttcacaata
    14461 tccgtaaagc ggatttgctc gtttaaaaac gcctcaacgg caatttcatt ggcggcattc
    14521 atcgctgttg tcgcatactg tccttctgca aaagcctcaa tcgctaattt taagttcggg
    14581 taacgggcaa aatccggctc gataaaggtt agctctttta atttaaagaa atctaatggt
    14641 ggcacaccag cattaattcg gtttggatat gccatcgtgt gagcaatcgg cgtacacata
    14701 tccgggttgc ccatttgagc aatcaccgag ccatcaatat aacgcaccat cgaatgaata
    14761 attgattgtg ggtgaatgat gatatccatt tcctcggctg ttgcattaaa caaccagcgg
    14821 gcttcaatat actccaagcc tttgttcatc attgtggctg aatcgactga aattttctta
    14881 cccatagacc aattggggtg agcaactgct tgagccggtg taatcgcatc aaattcattt
    14941 aatggcttag tacggaacgg tccacccgat cctgttaaaa taattttact gacacccagt
    15001 tcagcgagtg gacaaaaccc gactttttgc tgtgcctccg gtggaagcga ttggaaaatc
    15061 gcattgtgtt cactatctac cggtaagagc ttcgcaccgg atttacgagc ctcatcaata
    15121 aaaatttgcc cacaggttac cagcgattct ttatttgcca acaatacggt tttacccgct
    15181 ttcaccgcag aaagtgtcgg taataaccct gctgctccta caatagcagc cattaccata
    15241 tccacttcgg aatgtgcagc aagctcacaa atagctgctt gaccggctaa cacttcagtt
    15301 ttagacccca aagttgccaa tgccgttttt aatgcttgtg cggatttttc atcatccatt
    15361 gccgcaaaag tcggctgata ttgacgacat tgctccagca ttaattcaac attctttcca
    15421 ccaactaacg caaacacctc atattggttt gggtttgggt tatgctcaac taccgataaa
    15481 gtgcttttac cgatagaacc tgttgaacct aaaatcacta attttttcat tttgtttctc
    15541 tttatctaat gactgataag ggcgaaagat ttttcgcccc tacgccgtaa ttatgcggat
    15601 actcattcgt tgtttgtaaa ttgtttagca aaaacaaccg cttgtagatt aactcgaaag
    15661 ccgttttgct tcgataacat catctaattg agcgatacga gccaataatt tattcaaaat
    15721 ttcaacgttg ttaagctgaa tttccatatc aatggtggca aggctacgtt tcacatcggt
    15781 tcggctggaa acgcctaaca cgtttacttt ttcatttgcc ataattgcac tgacatctcg
    15841 cagtaagccg ttgcggtcat tggcaatcac tcgaatggtt aagcttaaac cagaggtata
    15901 gccttcgccc caatccgcct ccacaactcg ttctgggttg agactttgta attcaaacag
    15961 ttgctcacaa tctgcacggt gaatcgaaat ccctctgcct tgtgtgatgt aacccacgat
    16021 ttcatcaccc ggaatcggct ggcagcaacg ggcaatggaa tgcattaagt tccctacgcc
    16081 ctctacaatc acatagcctt tgcttggctt cggctgattg gtgtttttat tcacctgttt
    16141 aagtaccgct tcgtcttctt gttcggcggt cggtttaatt aattttgatt gcaagaagtg
    16201 cattaactga tgaagacgaa tatcgccacc gccaataccg gcatagagat catcaaactg
    16261 ttttaagtta tagcgaggca gagcatattc ttcaatttgc ttgagcgtaa aaccgaattt
    16321 ttccatttcg gcttcaaggg cctctttgcc gattggaata tttttctctc ggtctaattt
    16381 tttaaaccac gcaataattt ttgagcgagc tttgcctgta ttcacaaaac cggtattcgg
    16441 attgagccaa tctcggcttg ggttcggctc tttttgggtg ataatttcaa cctgatcgcc
    16501 catttgcaga tgataagtaa acggcacaat ccgccctgcc acttttgctc caatgcaacg
    16561 atgcccgatt tcactatgaa ttgcataagc aaaatcaagt ggtgtagctt tttgcggtaa
    16621 atcgatcacc tcgcctcttg gggtaaagac ataaacacga tcatcaaaaa tttgcgaacg
    16681 catttctgcc accatatcgc ctgaatcggc aagatcgttt tgccactcaa gcaatttgcg
    16741 tagccacacg attttttctt cgtagcccga acgccctgcg gttgccccct ctttatattt
    16801 ccagtgagcg gctacgccta gctccgcatc atcgtgcatt tgctgggtac gaatttgcac
    16861 ttcaatcgtt ttttcacctt cgcctaatac aactgtatga atagactgat aaccgttagg
    16921 ttttgggtta gcaatataat catcgaaatg ttccggcaaa tgggtatatt tggtgtgaat
    16981 aatgcctaat gccgtgtagc aatcttctaa cgttggcact attacccgca cagcccgaat
    17041 atcgaaaagc tgattaaatc tcaaccgttt tttctgcatt ttcttccaaa tgctgtagat
    17101 atgtttcggg cgtccataaa tctgcacatt cttgatttca ggacttaaac aagcggtcag
    17161 atctgctaca aaatttgcaa tataacgctc acgatccaca cggcgttcgc ccaattcaaa
    17221 tttagcaatt tgagtgtaag agacagggtg taaaatgcgg aaacagtaat cttccagctc
    17281 ccatttgagc tgcccgatac ccaatcgatt ggcaagcggt gcataaacgt gagaacattc
    17341 tttagcggcg agaacggtat cttcttcgga atggtggtta ctatcacgca ggtaggcaat
    17401 tcgttcggca agtttaatca ccacacagcg gaaatcgtcc accatcgcca acaacatgcg
    17461 acggatattg tcgatttgca gatccgacac attattggca ctaagctggc ggatattctc
    17521 catttcgagt acgcctttaa ctaagttttt gatgtcgtta ctgaaatctt cttttaattg
    17581 caccaagtcg atcacattgt gttttaccag aggaaaaagc attgcggcaa ctaagctgtc
    17641 atcgtccata ttcaagccgt gcaatacttc aaccatctca atgccatacc acatcagact
    17701 atagtcggta tccagttttt cctgtgcata actccacgca ttgtgaatct gctcaaaagt
    17761 cacaggagac atattcaggc ttgcacacca actcgacaac tcaaaattat tcggttcaag
    17821 ttgatgggaa taacgaattg ctaccataag gcctccttgt aaatttttgt ggaaattaga
    17881 ccgcttgtac agcttaaaat ggagttttat tatacgtgga aattcagaaa aaatttaccc
    17941 ctaatcgaaa atttaatcca agttatctta tcctctttac tccttaagag gtagcaacct
    18001 tgtgataaat caaacctatc aacacaatca tttgcacgac ttcacgctct tgattaaaat
    18061 acttgcgttt ttattttgat ctttattttt gtgggtagca atgacaattt tagctttagg
    18121 aatcaaccat aaaaccgcct ctgtgggttt aagagagaaa gtggcgtttg tagatgacaa
    18181 acgtaaactg gctcttgaac aaatccaaac gagcggattg gctgaaagcg tggtcattct
    18241 ctctacctgc aaccgcaccg agctctactt ccaccagcct aatatttctc cccgtgaaga
    18301 gagcgaggaa aatatccaat ggcgtgaaca atgtttccgc tggtttgccc aaattcatca
    18361 acttgatgaa agcgagttgc gtgaatgttt atattttaaa caaaatttag aagccgctaa
    18421 ccacttaatg aaagtggcaa gcggattaga ttcactaatt ttaggcgaac cacaaatttt
    18481 agggcaggtt aaacaagcct atcaatatag cgaagatttc taccaacatc agcaaaaagc
    18541 gatgtccacc aagctatcac gcttgttcca acgcaccttt gccaccgcaa aacgagtacg
    18601 ttccgaaacc gaaatcggca gccaagcggt gtcagttgct tatgcagctt gcggtttagc
    18661 ccgtcaaatt ttcgataatt ttggcaagtt acgctttttg ctggtagggg caggcgaaac
    18721 cattgaatta gtcgcccgct atttaattca acacggggcg aaaaatataa tgttggcaaa
    18781 ccgcacccat attcgggcgg aaatgttggc ggaaaaacta aacgctccaa tgcaaattct
    18841 gtcgttaagt gctttgcaac tcggtttaaa ccaagctgat gtagttatta gctccaccgg
    18901 tagccctgat accctaatca gtaaagaaat ggtggaaatt gcgcaaaaac aacgccaatt
    18961 tgacccactt ttgctgattg atattgccgt tcctcgtgat attgacgaaa aagccggcga
    19021 cttagagggc gtttacgctt atagtgttga tgatttacaa aatattatcc aacgcaatat
    19081 ggctcagcgt gaagaagccg ccaaacaagc cgctgaaatc gtcaaagagg aaagcaaagc
    19141 attttttgaa tggttaaaac aacagcagtc tagtgattta atcaaacgct atcgtcaaga
    19201 tgcggagcaa acccgccaag aattgttgga gaaagcccta caagcaatca gtcaagggca
    19261 agacagccaa aaagtattaa gtgagctaag ctacaaactc accaaccagt tactacacgc
    19321 cccaacccaa gcgttacaag caatggcaaa aagtggcaac tccaccggct tacaaagttt
    19381 ttcaagagca ttacggctag aagaatgatg ctcagcacat tcttttaggc atatttgcaa
    19441 aaaattgaac atttttaacc gcttgcggac gcacgccgtg cgtccctccc cgtgttcccg
    19501 atcttcttct aatttcaact tccccaaatt tccgctataa taagccaatt tttgcaacta
    19561 ttttgcaaat aaccggatat agaggataca aaatgactcc tgttgttgcc ctagtgggca
    19621 gacctaatgt cggcaaatcg actttattta accgtttaac ccgtactcga gatgccttag
    19681 tagcagattt ccccggctta acccgagacc gtaaatatgg tcacgctaat attgccggct
    19741 acgatttcat tgtaatcgac accggtggta ttgatggtac agaggaaggc gtggaagaaa
    19801 aaatggcgga acaatcttta ttggcgattg aagaagccga tgtggtgctt ttcttggttg
    19861 atgcccgagc cggattatta cctgccgatg tcggcattgc tcaatattta cgccaacgtg
    19921 aaaaaaccac ggtggtggtt gccaataaaa ccgatggtat tgatgccgac tctcactgtg
    19981 ccgaattcta tcaattaggc ttaggcgaag tagaacaaat tgctgcagca caagggcgtg
    20041 gggttacgca gcttatcgag caagtgttag ctcctttagg cgaacagcta aatgccgagc
    20101 aatttaacca agaacaagcg ggcgaaaatg ctaaaaatgt tgcaaatgaa gaaacagacg
    20161 aatgggatac cgaattcgac tttgagaacg aagaagatgc ggcattatta gatgaagcct
    20221 tggcagaaga agcagaagag tcgattgaag ataaaaatat caaaattgcg attgtcggtc
    20281 gtccgaatgt tggtaaatca acactgacca accgcatttt aggcgaagag cgtgtggtag
    20341 tttatgatat gccaggaaca acccgtgatt ctatttatat tccaatggag cgtgacggtc
    20401 agcaatatac catcattgat accgccggtg tgcgtaaacg tggcaaagtg aatttagcgg
    20461 ttgagaaatt ctcggtaatt aaaaccttgc aagccattca agatgcaaac gtggtgctat
    20521 taactattga ggcccgagaa ggtatttccg atcaggattt atcactactc ggctttattc
    20581 taaatgctgg tcgttcattg gtgattgtgg taaacaaatg ggacggcttg tcgcaagata
    20641 ttaaagatca agtgaaatca gagttagatc gccgtttaga ttttattgat tttgccagag
    20701 tgcattttat ctcggcattg cacggctcag gcgtggggaa tttgtttgat tcagtaaaag
    20761 aagcctacgc ttgtgcaacg caaaaaacct cgacctcaat gctgactcgc attttgcgta
    20821 tggctgctga tgaacaccaa ccaccattgg ttaatggtcg ccgagtgaaa ttaaaatatg
    20881 ctcacccggg tggctacaac ccaccaatta ttgtgattca cggcaatcag gttgaaaaat
    20941 tagcggattc ttacaaacgt tatttaagta attatttccg taaaagttta aaaattatcg
    21001 gctcgccaat tcggatttta ttccaagaag gcaataatcc ttttgccggt aagaaaaaca
    21061 aactgacacc aagccaatta cgcaaacgta aacgtttgat gaagtttatt aagaaaagta
    21121 agaaataata tctaacgata gcgcaagcgt ccacgcttgt gctaaaacta ataatggcac
    21181 aagcgtggac gcttgcgcca tcatttaaca gagaatatta actatgatga tgcaatactc
    21241 tccaaaattt aataatgcta aagtgctggt gcttggcgat gtaatgttag accgttactg
    21301 gttcggggca accaatcgaa tttcgcctga agcccctgtg ccggtggtga aagtacaaga
    21361 tattgaagag cgtgccggcg gtgcggcaaa cgtggcgatg aacatcgcca gccttggcgt
    21421 accggtaaca ttacacggct taattggcga tgatgatgcc gggcgtgctt tagataaatt
    21481 acttaattcc caccagatcc aaaatcattg tgtggtgatt aattcgcacc caaccatcac
    21541 aaaattgcgg attctttccc gccatcagca attattgcgg ttggattttg aagagggttt
    21601 tcacaatgta gattctaccg ccctacttgc aaaactttcg caagaaatta ccgcttacgg
    21661 agcattaatt ctctcggatt acggcaaagg cacattggaa tcggtgcaac aaatgatcca
    21721 aattgcccgc aatgctaagg tgccggtgct gatcgacccg aaaggcacag actttgaacg
    21781 ctatcgtggg gcaacattgc ttacaccaaa tatgtcggaa tttgaagcgg tggttgggca
    21841 ttgtaaagat gaggatgaga ttgtcagcaa agggttaaaa ctgattgccg actatgattt
    21901 atctgcacta ttagttaccc gttcggaaaa aggaatgacc ttacttcgcc cgaaccacga
    21961 gccttatcat ctgccgaccc aagccaaaga ggtttatgat gtaaccggtg caggcgatac
    22021 cgtaatcagt gtattagcca ctgcgattgc cgatggcaga gcctatgaag aagcctgcta
    22081 tttagccaat gctgccgccg gtgtagtcgt aggtaaatta gggacttcaa ccgtcagccc
    22141 ggttgagctg gaaaatgcga ttcaccaacg cactgccacc ggttttggcg tggtttctga
    22201 agccgaatta aaagcgattg tggaagaagc gaaaaaacgt ggcgaaaaaa tcgtgatgac
    22261 gaacggttgc tttgatattc ttcaccccgg tcacgtttcc tatttggaaa acgcccgtaa
    22321 attaggcgac cgattaattg tagccgtcaa taccgatgct tcggtaaaac gcctaaaagg
    22381 cgaagctcgc ccgattaatg atttagctgc ccgtatggca gttttagctg gtttagcctc
    22441 tgtagattgg gttgtgcctt ttgatgaaga tacaccacaa cgtttaatca gtgaaatcct
    22501 acctgattta ttggtaaaag gtggtgatta caagccggaa gagattgccg gcagccaaga
    22561 agtatgggca aacggcggcg atgtgcgagt attgaacttt gaaaacggct gttctaccac
    22621 taatgtgatc aagaaaattc aagcactata atggtaataa gcgaatggcg agagaaaaaa
    22681 tcattattga taccgagcaa tttcagcgca caatatctcg tatttcacat caaattattg
    22741 aaaaacacgg tgatttgcaa gatgtagtga ttgtcggcat caaacgtcga ggagctgaaa
    22801 ttgccgagtt aatccaaaaa cgcattgaag atttagctca aacacgcttg ccttatatgg
    22861 cgttagatat tacgttctac cgtgatgacc tagacttaat ttatcaagat cccgtattta
    22921 tgggagccga taatcagcta aatctgaacg gtaaaaaagt gattttggtt gatgatgttc
    22981 tgtttaccgg cagaaccatt cgggcggcac ttgatgcact cttagatttt ggcagagctg
    23041 ccagaattga actggtgatt tttgtcgata gaggtcatcg ggaattgcct atccgagccg
    23101 attatgtcgg aaaaaatatt ccaacggcta aaaacgagca aattcaggtg caaacgctct
    23161 cgtatgatgg gattaaccaa gtggtcttag ttcctgctgc caataaagaa agtgcttaaa
    23221 ttaagtgaac cttttcgcta aacttatctc taaggaaaag ttactgctta attacctcca
    23281 ttaggattgt tataaaaatg aaattagtca aatcattatt tgctgttgcc gttgcaacaa
    23341 tgagtcttac ccaaaccgcc cacgcatttg aagaaagagt ggtggcgtta gtgaatgata
    23401 cccctattat gcaaagccag gttcaacgtg tgcttggcaa gaaaaaggcg acagaaactg
    23461 ctcaacgtgc ggctattgat caagtgattg atgatatgtt ggtgcaacag gcaatgaaag
    23521 aagccggcgt taaagtcagc ccagctgcgg taaaccaagc ggttgaaaat gtagcaattc
    23581 aaaacggcat tacttacggt cagttattag atgcgttaga ttatcaaggt attacacttg
    23641 aacaattcaa acgtaatatt gctcaacaaa tggcaatgga acaagttcgc catatcagca
    23701 ttagcaaatc gattcaggtt gcaccacagc aagtgcaagc tctggcgaaa gagttaatgg
    23761 cgaaagataa agcagcaggc aaattgaaaa ccgtttctgg caaagagcat cgcattagtc
    23821 atattttgct caaaaccaac ccgattttaa atgatgctca agccaaagca aaacttagca
    23881 gcttaactac tgatattaac tcaggcaaaa tcagctttga agaagcggca aaaacccatt
    23941 ctgttgatta tgcctcaggg gcagacggtg gagatttagg ttttaacttc ttagaaattt
    24001 acgatccggc atttgcgaat gttgcatcaa aaatcaaacc aaaccaaatt tctgctccgt
    24061 ttaaatcgca atttggttgg catattttaa aagtcaccga cactcgtaac ggagacagaa
    24121 ccgaagatgc ttaccaccaa cgtgcttatc agcaactggt gaacaagcaa gccgaagaag
    24181 cctctaaaga ttgggtaaaa gcgcttagaa aaaccgccaa tattcaatat gtcggtcaat
    24241 aattaaaatt tttaagaacc tcgcataatg cggggttctt ttcttatttc aaaaaggata
    24301 acaatgagtt ctacaaattc caaaaaacat ttaggtcata ccgctcgtaa gcgtttcggg
    24361 caaaactttt tgcacgatat gaacattatt cacagcatag tttcagccat taacccgaaa
    24421 aacggacaat ttttattaga aatcggtccg ggtttaggag cattaaccga gccggttgcc
    24481 gaattggtag ataaactcac ggtggttgaa attgaccgtg atttagcaga gcgtttacgc
    24541 caccacccgt ttttaaacca aaaactcacc attattgagc aagatgccct acgctttaat
    24601 ttccgtgact attttaacca actcaactta gacgaagact cggtgcgtgt gtttggtaac
    24661 ttaccttaca acatttctac gcccttgata ttccacctgc tgacctttca cgacttaatt
    24721 caagatatgc actttatgtt gcaaaaagag gtggtgaaac gtttatgtgc cgcgccaaac
    24781 agtaaagcat acgggcgttt aaccattatg gctcaatact attgccaagt aattccggtg
    24841 ctggaagtgc cgccaaccgc ctttaaaccg gcaccaaaag tcgattctgc ggtagtgcgt
    24901 ttagtgcctt ataaccaact gccttatccg gcaaaagatg tgtattggct caatcgggtc
    24961 acgactcaag cctttaacca acgccgtaaa accttacgca atgctctttc aaccctgttt
    25021 agtgcggagc aactggacgc cttaggcgtt gatttaaccg cccgagcgga aaaccttacc
    25081 attgccgatt atgtgcgttt ggctaactgg ctacacgaca acccgccggc aattgataca
    25141 accgaagaat tggttgatga agattgctaa tcaataaatt acaagcggtc atttttctac
    25201 caagatttgc aaatctttag tgaaaaatgg ccgcttgttt aaatcagctt tacttgtttt
    25261 gctgttactt agttttgatg ttacttggtg ccgaaaattt tatcccctgc atcgcctaag
    25321 cctggaatga tatagccgtt ttcgtttaag tgactatcga ttgaggcggt gtagagttca
    25381 acatctgggt gggcggcttc taatacttta atcccctctg gagcggcaac taacactagc
    25441 actttaatct gcttacagcc tgcttttttc aggaggtcaa tggttgctac cattgagcca
    25501 ccggttgcaa gcattgggtc ggtgataatg gctaaacgct catcaacatc gttggctagt
    25561 ttggagaaat acggcacggg ctttaaggtt tcttcgtcac gataaatccc aaccacacta
    25621 attctggcac ttggaatatg ctctaacaca ccgtccatca tacctaagcc agcacgcaga
    25681 atcggcacaa cagtcacttt tttgccttta attcgctcaa ccgcaatttt ctctccgctc
    25741 cagccattga tctccacact ttccatttcc agatctttgg ttgcttcgta agtaagcaga
    25801 ctaccaattt cattcgctag ctcacggaaa tctttggtgc tgatttccgc ttcacgcatt
    25861 aagcccaatt tatgctttac aagtgggtgt tttacttcaa caattttcat tttaagctcc
    25921 ttaattagtg taatttaagc cttattcacc tttgctaatt cttcgtctct taacactcgg
    25981 cgaagaattt tgccgacatt actttttggt aattcgtcac ggaattcgat gtctcttgga
    26041 attttgtagc cggttaagaa ttgacgacaa taggttctca attcatctcg agttaaactt
    26101 tcatcttttt tggttacaaa aattttaatg ctttcgccgg aagcctcact cggcacacca
    26161 atcgccacta cttcattcac ttttgggttt tgggaaatca cttcttcaat ttcggttggg
    26221 tacacattaa aaccggagac aataatcata tcttttttac gatcaacaat gcgtaagttc
    26281 agatcatccc ccattaccac aatgtcaccg gttgccatcc aaccatcttg tagcacttgt
    26341 gccgtttcat caggacggtt ccaatagcct tgcattactt gcggcccttt caccaccagc
    26401 tcgccccgtt cgccgagtgg tacttctttg ccttcatcat ccacaatacg aatgtcagta
    26461 tttggcactg gcacgccaat agagccggaa tattccaccg aatcgccacg ggttgcacta
    26521 atcagcggag aacattctgt cattccataa ccttcgataa tatgtacgcc ggttaaatcg
    26581 tgccagcgtt ttgccacgct agattggatc gctgcaccgc cacctacact taattttagg
    26641 tgtgaaaaat caacctcttt aaaactttcg tgattaagta atgcattaaa caatgtattt
    26701 accccagtaa tcactgaaac tttgtacttt tttaattctt taataaagcc tggaatatcg
    26761 cgcggattag taattaataa tccggtaagt cctaattcga taaagagcaa gcaattcatc
    26821 gaaagtgcta agacgtgata cagcggtaaa ggtaccaatc caatcatatt ggcagaatta
    26881 cccaacaatg gctcaacaat ccattttgcc tgcatcatat tagcaattaa gttttgatga
    26941 gtcagcattg cccctttcgc tacacctgta gtaccgccag tgtattgtaa aaaggcgaga
    27001 tcttggctga caatattcgg ttttacatat tgacgctgtt tgccaatgct taaggcttca
    27061 cggaagctga ccgcatgtgg caatttatat ttcggcacta attttttgat gtatttcacc
    27121 acaaaattga ctaaggtgcg tttgccgaat gaaagttggt cgcccattcg ggtcaaaata
    27181 acgtgcttaa tctcagtatt aaagacaatt ttttctaagg tagccgcgaa gttcgagact
    27241 atcacaatcg ctttcgcacc actgtcattt aattgatgtt ctaattcacg cggcgtataa
    27301 agcgggttga cattcaccac cacaagcccg gcacgtaatg cgccaaataa agcgatcgga
    27361 tattgcaata aattcggcat catcaatgcg attcgatcac cttttttcag gcgtaattca
    27421 ttttgcaaat aagcggcaaa cgagcggcta cgttcttcca atttacggaa cgttaaaacc
    27481 tgccccatat taatataagc tggaatatca gggtggcgtt gtatcgctgc ttcaaacatt
    27541 tccactaaag attcatattg atctgtttca atcgtttttg gtgaataagg cggataatta
    27601 tttagccata ttttttccat tatagtctcc taaaacctta cctaaaaaga taggtgaaaa
    27661 atttgcttgt ttgctgtgtt tatgaatcaa ttttcttaca gcccagaatg ttatacctgt
    27721 tgaaagcaac attataaata aaacaataag ctcatttgca aaaaattgcg aaaatccgac
    27781 cgctgtcatc acaagaacca gcagaggaat gccataaatt aaaaacacgc ttttgagcaa
    27841 gatattttca gctaacccta gttcaacctt atcgcctact tttaggggtt catctacctc
    27901 aatacgaaaa cgagggtcaa ttttttcacc tgctaatgct gataatgctt tgctgccaca
    27961 agcagactga gctacacagc caccacagct acttttagca taacattgta caatagccac
    28021 accattctga tattccacca ccgtagcctg ttcgatcatc atggctgagc tccttcctta
    28081 aaaccaatat tgttcataat ttgcttagcc gattgcaacg gcacatcacc aataatcaca
    28141 atatctctat cattaatcgt ttgatttaaa atcgttaatt taccttgttg ccaagcgtac
    28201 tcattaaaac tcacgccttg gctaggcata atataaatag ttaatgtaga taaaccatca
    28261 ctatatactc tgctatctac atactcactt tccacttgat atgtcgcacc tgtgactgtt
    28321 tgttgctgaa ttaacctaaa ccctgtcggc aaccacttca cctgccaacc atcagactta
    28381 ggaaattctt cttttttcac tgtcgttaat gttggtaaca ttagtgattc tattacattt
    28441 gcaatcctat ccaattctgg agattgataa agttgcaaaa ctctgaattc ttctaatacc
    28501 aaattatcca tattcaagac ttggctttta agtaataaat gtgtttcttc atcaatccat
    28561 agtatatagg catatcttaa agtatcattt ggcttcatac gaattacctt tgtaattcgg
    28621 tcagaaatac gatctttacc agcatctaaa aaggcataac cttttaaatt tgcataatct
    28681 gtgtaaagta cattagggag gttatctaaa atctgtgaac tatttaaact aaaaggacga
    28741 aaattatacc ccaaatagct gaccttgtta ttttttaaaa taatttcttc tcggctgtga
    28801 tctaaattca gtaattgggc atattcttta ttatgggtaa aagcatgacg taaacggaaa
    28861 gattctgtat cacttccctg ttgaagaata taaact
//
LOCUS       DANLYI010000008        28884 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.008, whole genome shotgun sequence.
ACCESSION   DANLYI010000008 DANLYI010000000
VERSION     DANLYI010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 28884)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28884)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28884
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.008"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            56..1501
                     /gene="aldA"
                     /locus_tag="PXD81_000266"
     CDS             56..1501
                     /gene="aldA"
                     /locus_tag="PXD81_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HDL5536337.1"
                     /translation="MVKQHKMYINGEFVPALDGRTLAVYNPATEKAISEVPRGGADDA
                     KIAIDAAEAAQEAWERLPAIERGKYLRQIAVGIRERADEIARTISEEMGKTLSLATVE
                     VHFTADYLDYMAEWARRYEGEIIQSDRPNEHIFLYKKAIGVTTGILPWNFPFFLIARK
                     AAPALVTGNTIVIKPSEDAPNNAILFSEIVHQVGLPKGVFNVVTGYGNEVGPELSGNE
                     KIGMVSFTGSQFAGQKVMEAASKNIIKVNLELGGKAPAIVLDDADLELAATAVVNSRI
                     INSGQVCNCAERIYVQSSIKEQFVQKLVEKMQAVKYGNPLEQQDIDMGPMVNEKGLHK
                     VQEMVTHALHQGGNLVLGGNVVEGTGYYFEPTIIDNVDNSMDIMRHEIFGPVIPVATF
                     DTIDEAIRLANDCEYGLTSSVYTQNINKAMKVISRLKFGETYVNRENFEAMQGYHAGW
                     RKSGIGGADGKHGLEEYLQTQVVYLQFDDKV"
     gene            1757..2404
                     /locus_tag="PXD81_000267"
     CDS             1757..2404
                     /locus_tag="PXD81_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005613280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1440 domain-containing protein"
                     /protein_id="HDL5536338.1"
                     /translation="MSIFQQTNPNRRRYGLAVFIGIIAGIISAFVKWGAEHPFPPRSP
                     IDLFTAACPQPVLDAMNAAADQVAAKTIALQECSRAFLNPPHVFLRDYIGIDPTEAAF
                     TFADHAFNWIGVTHMIFSLVFAIGYCIVAEIFPKIKFWQGIGAGIIANICVHYITFPA
                     LGLTPPVAEWPLYEHISELVGHIFWFWTIEVIRRDLRNRITREPDAEVPLDQPFR"
     gene            complement(2537..3913)
                     /gene="trkA"
                     /locus_tag="PXD81_000268"
     CDS             complement(2537..3913)
                     /gene="trkA"
                     /locus_tag="PXD81_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HDL5536339.1"
                     /translation="MKVIILGAGQVGSTLAENLVSEDNDIVLVDNDPTRLDKLKDKHD
                     LQVIRGEYASPQTLRDAGAGDADLLVAVTNSDEVNMIACQIAYTLFNVPTKIARIRSA
                     DYVRERERLFNDDVLPIDHIIAPEILVKEDILRLIHYPGALQITHFADELVSIVNVKA
                     YYGGPLVGYPISALRDHLPHIDARVVAIFRQDKAIVPQGSTIIEAGDEVFFICATQHI
                     RAVMSELQRLDRPHKRIMIVGGGSIGSSLARDLEDQYRVKIIERNPVRAEKLAEKLSK
                     TMVLTGDASDEELLFEEHIENIDLFLAVTSDDEANIMSALLAKRLGAKKVIILVQRPA
                     YLHLIQGGTIDIAISPQQATISALASYVRKGDILQVSSLKLGVAGAVEIIAHGDATTS
                     KVVGRQIRELKLPQGAIVGAVVRGEEVIIAHKSTMIAENDRVIIFVNDKKQIDEIEKL
                     FQLGVFFL"
     gene            complement(3913..5244)
                     /gene="rsmB"
                     /locus_tag="PXD81_000269"
     CDS             complement(3913..5244)
                     /gene="rsmB"
                     /locus_tag="PXD81_000269"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015431887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HDL5536340.1"
                     /translation="MKKQSFKALSSRAISATIILQVLDQGKSLSTLIPDAQKQLEPKD
                     LPLVQEITFGVCRVLPRLESIIKLLVEKPLKGKTRLVHCLLLVGLYQLLYMRVPAHAA
                     VDEVVNATKTLKLDSFRALTNGVLRRFLREQEEILAKVDKHWQTLHPEWLVNKLKKAY
                     PNWREIVEANNQRPPMWIRVNQQHIKTNDYAALISELVEPEFAKNSENPTACVPKCAL
                     LLERAVNVNQLPHFEQGWATVQDAHAQWSAELLGAENGETVLDACAAPGGKTTHILEK
                     APKAKVMALDIEESRLSRVRENLVRLGQIAQVICGDASKPEEWLEEGVLFDRILLDAP
                     CSATGVIRRHPDIKWLRKEGDIAELATLQGEILKALWQRLKPNGILLYATCSVLPQEN
                     SEQIQRFLQDTPNAKQLEIDFNGEKVLEKQFFPQPNGGDGFFYAKLQKVAE"
     gene            complement(5296..5721)
                     /locus_tag="PXD81_000270"
     CDS             complement(5296..5721)
                     /locus_tag="PXD81_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HDL5536341.1"
                     /translation="MYNKLLIAIDLADLKSAKYVVDTALQMTAKNPNAVYRVVSIIEP
                     VDNSLISAFLPKNFDKEVVAEANQKLHEFTKNHFPEGSKVQHIVAYGTIYEEICRIAD
                     EKSVDLIMMLASSKPNAKGLSSNTVKVARNTTKPILVLR"
     gene            complement(5814..8462)
                     /locus_tag="PXD81_000271"
     CDS             complement(5814..8462)
                     /locus_tag="PXD81_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter permease"
                     /protein_id="HDL5536342.1"
                     /translation="MSVESKVDYDDLQDMVASNDSGGRTPSGLAKKAIVYVAILWSVF
                     QIYYASPLPFVFQEWLTHAGINLNVVVDDTKARSIHLAFAMFLAFLSYPAFASSPKHH
                     IPKIDWVFAIVGAGLALYYIFFYEGLVKRFGAPNTQDIIAGCLGILLVLEATRRSLGL
                     PLVIIAIVFLIYNFFGQYFPADWIVSHRSGSLSQIINQQWITTEGVFGVALGVSTKYV
                     FLFVLFGALLDKAGAGNYFIKTAFAYLGHLRGGPAKAAVVSSALTGLISGSSIANVVT
                     TGTFTIPMMKRVGFTKEKAGAVEVASSVNGQIMPPVMGAAAFLMIEYVNMPYSQLITH
                     AFLPALISYIALVYIVHLEACKMNLQGLPRAEEPSPILVFLLRTVASILVIVGLAIGV
                     YYGLGWIRDVAEDYAFMIVCAFLGLWYLLAIRRVASYPDLEADDPNSEIVSLPKRKPT
                     VNAGLHYLIPVVVLLWCLMVEMLSPGLSAFWGTITLIFIQLTQRPLLNFFRKESVTKE
                     IIKQGVRDVVDSLEAGGRNMTGIALATATAGIIVGVVALTGFGVQLSSVIELLSMGNI
                     VLMLILVAVFSLILGMGLPTTANYIVVSSLMATVIMEVGRQNGLIVPLIAVHLFVFYF
                     GIMADVTPPVGLASFAAAAISGGSPINTGMVAFKYSLRTVILPFLFIFNTDLLLIDLR
                     YPGHGWVVFITATVGVLAFTSATMQYIVTKNKWWETALLVVAAFALFRPGFFMDRIYP
                     SERHIEPVQFEEQLLASKVGQNVTLKVEGLNPYGKEIEFYAQLPVPEGATGEERLKNL
                     GLTLIQTDEKIKVDGVETPKVVIDMVEMDSPAAKAGLNWDQTIHYIAVPQDAPSKDWI
                     FIPALLLVFGIVANQRRRVKKQCVIA"
     gene            complement(8664..9626)
                     /locus_tag="PXD81_000272"
     CDS             complement(8664..9626)
                     /locus_tag="PXD81_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HDL5536343.1"
                     /translation="MKKLVKLSFLASLVMGTASVQAAETFVTIGTGGQTGVYYVVGQS
                     ICQLVNRDSAKTGLKCNAPSTGASVANLNAIAAKEMDMGIAQSDWQYHAYNGSSSFEG
                     KKNDKIRAIFSLHPEPFTVMARTDSGIKSFDDLKAKRVNVGDPGSGTRATMNVILAAK
                     GWTDKDFKVASELKPAEMASVMCDNNLDAITYNVGHPNGALKEAAASCDAKLVPVTGP
                     EIDKLVADHSYYAKATIPGGLYKGSDEPTETFGVYATLVTSADVDADKVYNVTKAVFD
                     NFDRFKRLHPAFEHLKQEEMIKNALSAPLHEGAIRYYKEKGWIK"
     gene            complement(9810..11657)
                     /gene="selB"
                     /locus_tag="PXD81_000273"
     CDS             complement(9810..11657)
                     /gene="selB"
                     /locus_tag="PXD81_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005608929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HDL5536344.1"
                     /translation="MIICTAGHVDHGKTSLLQALTGTNTAHLPEEQKRGLTIDLGYAY
                     LPIENDILGFIDVPGHQRFLSNMLAGLGGIQHALLVISAEEGIKPQTEEHLDILHLLN
                     FQHIMIVITKADRAEETQISHLIEQVKTSYPFLAEAKTFVTSAKTQQGIEELKAHLIQ
                     LNQQHTQTHKPFRYAIDRVFNVKGAGLVVTGTTVAGKVSVGSELFLSNGKKVRVKAIH
                     AQNTPSETGVAGQRLALNIANVEKEEIKRGDWITELEPKFATDRITVQLTANQAFKEN
                     SIVHLYHFASHITGKLNLLEGQQAVKNQQFFAEVILDEPLHIAVGDKLIIRSGDDSLT
                     LAGAEVLEIHSPKRHKRTEARLALVKNLAKTTACNDFATRLDLYLQNRAMDLSLLLWA
                     EQCFAEDIAKLGFDVSSKWLFNSDFKAQAQQRVLEKMAEYHLQHQDQIGVTKARLYRI
                     ALLDLPENLANQFIEDLVEQKALVNSRGWLHLPEHRIELNADELQIWQQIRPLFEATN
                     QALWVRDIASQLSIDETQMRNLLYKVGKLGYLIPIVKDRFLLSEQIGQFATLIKDFIA
                     QNGSISVNQLRDELQYGRKLTVQLIEYFDRSGFLRRKGDVHLLRDSETF"
     gene            complement(11667..11963)
                     /locus_tag="PXD81_000274"
     CDS             complement(11667..11963)
                     /locus_tag="PXD81_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDL5536345.1"
                     /translation="MMKFDYNIVWLQKAEIKLFYQADYILQQSQNPIIAEQFYDAIKS
                     EVDKLSFTADVYRLRKKKEIPILNGKYQVKFLIGQENVYIVDFKSARQNSYSFL"
     gene            complement(11960..12136)
                     /locus_tag="PXD81_000275"
     CDS             complement(11960..12136)
                     /locus_tag="PXD81_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5536346.1"
                     /translation="MELILAQDLAKLRTDKPQSFYTNDELDLLHQLENGLIETKSHAQ
                     VMEDLRKKLGITEK"
     gene            complement(12205..12837)
                     /gene="pdxH"
                     /locus_tag="PXD81_000276"
     CDS             complement(12205..12837)
                     /gene="pdxH"
                     /locus_tag="PXD81_000276"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005575912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HDL5536347.1"
                     /translation="MDLHHIREDYTKKVLSIRDCHSNPITQFEQWLSEAINAKVNEPT
                     AMNIATVAANGRPSSRMVLLKEVNSQGFVFFTNYQSRKGWEIADNPFGCLTFFWAELQ
                     RQVRIEGKIAKIPEAESDAYFDSRPYTSRLGAWASEQSQPIENYKALLLKSAQFALKY
                     PLSVPRPPHWGGYILIPDSIELWQGRSSRLHDRIHYWLENNEWKKERLSP"
     gene            complement(12895..14277)
                     /locus_tag="PXD81_000277"
     CDS             complement(12895..14277)
                     /locus_tag="PXD81_000277"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HDL5536348.1"
                     /translation="MQQLFRQIPSLDLLLKKPQAVELCQKFAYSSVAEQARSLLTQAR
                     QFISQQQALPDFIADENLFFIRLEQKVQALSEVKIRSVFNLTGTVLHTNLGRGLWSEK
                     AIQAATNAMRNNVALEFDIEAGKRSHRDNYISELLQILTGAEAACIVNNNAAAVLLML
                     ATFAQGKEVIVSRGELVEIGGAFRIPDIMAQAGCKLVEVGTTNRTHLKDYRNAITENT
                     AFLMKVHTSNYHIEGFTSSVSEEELVALGKEFNLPVITDLGSGSLTDMQALNLPAEPK
                     VQQKVAAGVDLVSFSGDKLLGGPQAGIIVGSQAMISQLQQHPLKRVLRCDKVILSALE
                     ATLRHYLFPEQLTDNLPTLHLLTQPLETLQHKAERLKQALHKRLNSAYILQIELSLAQ
                     IGSGALPTETLPSVAVTISAEKQSDLIALEQQFKTYPSPIIGRFAQQKFWLDLRSVAE
                     FDKLIEMLEY"
     gene            14345..14439
                     /locus_tag="PXD81_000278"
     tRNA            14345..14439
                     /locus_tag="PXD81_000278"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:14379..14381,aa:Sec,seq:tca)
     gene            14646..15335
                     /locus_tag="PXD81_000279"
     CDS             14646..15335
                     /locus_tag="PXD81_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HDL5536349.1"
                     /translation="MSTLSRDSILSQIQNGLIASCQPVDDGPMDKPEIVAAMAAASVI
                     GGAKGIRIEGVDNLKAARAAVNVPIIGIVKRDLPDSPVRITPFLEDIDALAEAGADII
                     AVDGTFRTRPVSIEEAVKRIHEKGCLAMADCSTLEEGLHCQQLGFDIVGSTMSGYTGG
                     EMPDEPDYQLVKDLKAAGCNVMAEGRYNTPELAKTAIEIGADYVTVGSALTRLEHIVS
                     WFADSVKSAQK"
     gene            15345..16247
                     /locus_tag="PXD81_000280"
     CDS             15345..16247
                     /locus_tag="PXD81_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HDL5536350.1"
                     /translation="MRCLAIDIGGTKIATAIVQHNQVSQRQQIQTPTDKPQADQAGSL
                     HQAIGEIMQHYQGQFDFVAVASTGIINKGVLTALNPKNLGGLAEFPLKQSIEKHTDKP
                     IALLNDVQAAVCAEYLNEDQQAVKNFVFITVSTGVGGGIILNGELQIGSNGIAGHIGH
                     TLADPNGPLCGCGRVGCVEAVAAGRAIEAVSSQWEKPCSPKEVFERFRQGNPQAVELV
                     QKSAKAIANLIADLKISLDIQKVVLGGSVGLAEGYLPQVSNYLAEMPSVYHCVLENAL
                     SGQDAGLIGAAWWAENQLNQGFTL"
     gene            16272..17150
                     /gene="nanA"
                     /locus_tag="PXD81_000281"
     CDS             16272..17150
                     /gene="nanA"
                     /locus_tag="PXD81_000281"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005718701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HDL5536351.1"
                     /translation="MKNLKGIFSALLVSFNEDGTINEKGLRQIIRHNIDKMKVDGLYV
                     GGSTGENFMLSTEEKKEIFRIAKDEAKDEIALIAQVGSVNLKEAVELGKYATELGYDS
                     LSAVTPFYYKFSFPEIKNFYETIIRETGNKMIVYSIPFLTGVNIGVSQFAELFENEKI
                     IGVKFTAGDFYLLERLRKAFPNHLIYAGFDEMMLPATVLGVDGAIGSTFNVNGIRARQ
                     IFELAQQGKIKEAFEIQNVTNDLIEGILGNGLYQTIKGLLELEGVQAGYCREPMTKEQ
                     TPAQKEVTKQLKAKFL"
     gene            complement(17211..17417)
                     /locus_tag="PXD81_000282"
     CDS             complement(17211..17417)
                     /locus_tag="PXD81_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5536352.1"
                     /translation="MDNKQTNGFNDIIVKADITQNIWSTGFYDIGRELKSENTRQEKV
                     RLKYNGDTLHRGRKSRMASLIASR"
     gene            complement(17375..18013)
                     /locus_tag="PXD81_000283"
     CDS             complement(17375..18013)
                     /locus_tag="PXD81_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5536353.1"
                     /translation="MKKILLLVLLLTLATQADEGNNTQVCAEQQHQIIAHYFKISQEK
                     LSARTLASACKTAPNNSTETLVSYAVIEPKYSKENYQNDENTPKWTLFVARVNDNAIL
                     QSWEQDMEIDASIDVNADSLKIDTARYILNDKVRAFGVRFNNAARGPSAPEYSANDHL
                     MLFVPEKEKLKLVFDYPMTFWSILDDKETKTETQTWYCKWITNRPTASMILL"
     gene            complement(18255..18974)
                     /locus_tag="PXD81_000284"
     CDS             complement(18255..18974)
                     /locus_tag="PXD81_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL5536354.1"
                     /translation="MSKPNIYDESFSERMKWIAKNSFKDNYSEFARAVGVAQASLSRW
                     VKGEADPSRSNLVKIAEVSNVSLEWLATGKESKPQSENTSIVAREFERLEKMADDIVS
                     MINSFRTINVSAGFGSFNEGVTKPDGQEPYADSLLQSLGVKAHNCGVFWANGTSMHPT
                     IADSDQMLVDFSKREPRGDDKIYLVQNGESVWVKRIRKEWDYIELISDNESYRPIQIT
                     AEEAQNLQIIGQVVHIGHSLV"
     gene            19151..19429
                     /locus_tag="PXD81_000285"
     CDS             19151..19429
                     /locus_tag="PXD81_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL5536355.1"
                     /translation="MSVLNDVKKTAQRDWHKADILAELRKKGWSLRSLAKAGNVSHNT
                     LKSVLDKPYPKMERLVANAIGVAPEVIWAGRTAERIERNRRPTLTNKF"
     gene            19474..20277
                     /locus_tag="PXD81_000286"
     CDS             19474..20277
                     /locus_tag="PXD81_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL5536356.1"
                     /translation="MSELSLKTHYSVAELLSFKLSSLPSAHKNVLEKAIRENWQSQKR
                     KGRGGGVEYELISLPTEVQQEIRTKLLKLLPAEISKGELSVVRQNIDLEQITDKQLST
                     ADARIMVVRWFLMQEVQLGLSRTKTLDQVIAAVASSEIPAEICKAIMAGNSKAGGKLK
                     LSKRTLHSWVLAYEAGENSAERLKQMIPLKTQKRAVPERCGWLQAFLPFYQTFSNVAL
                     TQAYAQFAMQYEGEDLPTESQVRYALKQLPDYVVQQGRKLPVKKIRLAR"
     gene            20244..20756
                     /locus_tag="PXD81_000287"
                     /pseudo
     CDS             20244..20756
                     /locus_tag="PXD81_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251293.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
     gene            20767..21141
                     /locus_tag="PXD81_000288"
     CDS             20767..21141
                     /locus_tag="PXD81_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL5536357.1"
                     /translation="MSIVYEGSCVLEVDGVEIEITKLDVKTVTGRKLVKTMNSSGRAR
                     GYSQGIAEYTLSVTAVEPQDGSVIDWDNIKNAKLTKYPLDNSEKRTSYLGCFTTDVSE
                     SYTVDGESVRDIQLNALRKVNE"
     gene            21141..21509
                     /locus_tag="PXD81_000289"
     CDS             21141..21509
                     /locus_tag="PXD81_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5536358.1"
                     /translation="MKITLNLGIEYNGKIRRDCTVRLLTMGGQCTAQEMIADLGFEEN
                     SEALSKREQTLVDMAYLSQQVTFDGIYQGNVTAQFLFDNLATEDYWQLIEATLTLRKK
                     RLENGVSQSESESPANPDLA"
     gene            21539..21727
                     /locus_tag="PXD81_000290"
     CDS             21539..21727
                     /locus_tag="PXD81_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5536359.1"
                     /translation="MILLSKFAITAEQVWQMPLLEVFAWIESYLESEGVKTPHSPMGA
                     NSEVKRESYVFTRCGKIE"
     gene            21780..22775
                     /locus_tag="PXD81_000291"
     CDS             21780..22775
                     /locus_tag="PXD81_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HDL5536360.1"
                     /translation="MAKNMKVQLELTAKDTASPVLNQAAKTAEKAFKNTERAAQKSGN
                     AQTQAAQKAANATESSNRKIEQSYRNARRVAADVSRARETLGVRAENAIQREIEQTRA
                     AYDRLKRSGVASQNELRRASEATKQRVRELNAELGKTSFGDKAKGFGRGLMSAGVGIA
                     AGAAMARQPAKNQMEFDRQLAMASNTAFSDRDVKGRIEGKKELLAAVQEAVKVGGGTK
                     EDALLTLDTLIASGTMKAETAMSLLPTLQKGAVATGASSEDMAKIAISSMQQFGIKEE
                     DIGRALDMAVAAGQAGNFELADMARWLPQQMAAGNTARHWAVYYRLYRMTQTADG"
     gene            22724..23281
                     /locus_tag="PXD81_000292"
     CDS             22724..23281
                     /locus_tag="PXD81_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015586972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2313 domain-containing protein"
                     /protein_id="HDL5536361.1"
                     /translation="MGGLLPPVSYDPNGRWLALSLEVEGRELDRIDARAKTLIDAVDV
                     STGIFIDDWERVCGLTANSSLPLETRIERVIAKLNQLGGLSIGYITQVAKELGYCIKI
                     SEPQPFIAGLSRAGDMLWHKDIMWTFFVDVCNPTDDYKRFRASVSMAGDALMGRYKDP
                     ILESLLEEVKPAFSRVWVRYLGDIE"
     gene            23278..25338
                     /locus_tag="PXD81_000293"
     CDS             23278..25338
                     /locus_tag="PXD81_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015483985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL5536362.1"
                     /translation="MKQVMNPINTPTQRFKDGNPATGEYGTIVTAEFLNNVQDSVINT
                     QQELHSVLAEAGIEANDEQVNQVAKAIKKIAGDATRDNFNALANPDGYKHIGRCKSVA
                     ELRTIRPTEHGQRILVDAYYEDGTTGGGEFVADLQDLVTPDDGGVCFVVNNNGGRWKR
                     VDLSHLTLFDFGAVGDGVTNDESAFVNAMRYSQFFIENGTFRINNAVNSVRDNVQILG
                     NKTGKLVLGAGIQQAEAEVFNINHSNYFISGFCIETPNKAIGIRFKSLDDAGVKNLHI
                     DNVVFNGTFYGVRAGESIQADTNYPTDNVIVQNCQSYCGSGNAGHYLCTKVKGVRFFN
                     NIAIGGRNVSAYGATSCSDIFILGNREQGMAVTTVDVEAGVQVEDVQTGECVVANNIC
                     SHDIWMSGAKNAIINSNLCRRLRVTSGNPTDTGSNNVEFSNNQAKSIAISKYGSFDNP
                     SLFIDNINFINNILEPSEFNQDVYIDGTILKQCQFINNRCISKSKQYSVRLARNNPCL
                     NLTFKSNKLSDNPAIISGKQGVIFCDDLSLGVNKHRLRLRFSSPINFTVNQEWQKMPF
                     DRVLNDINGHVDIVSGQFIARTGGTFHLKGKITVTNSSEDVLNLYQLRLLINNEEKER
                     LINQSVSPKNSKSNTNGMTTINFDTVVYLKVGDIVDLQYKTTHELTLLSDSFLSQIYV
                     TQEF"
     gene            25568..25786
                     /locus_tag="PXD81_000294"
     CDS             25568..25786
                     /locus_tag="PXD81_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5536363.1"
                     /translation="MESHKTGNIITEQASKQASKQASKQASKQASKQASKQASKQASK
                     QASKQSVAQFKQAPLPFTGQKRMFLKHF"
     gene            complement(25969..27564)
                     /locus_tag="PXD81_000295"
     CDS             complement(25969..27564)
                     /locus_tag="PXD81_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL5536364.1"
                     /translation="MRLSQLSLSVLLLSASSLVAAAPVNTLINCVATPPMKLSPAITN
                     DANDFNASSQQIYNRLLAFKAGELTVEPSLAERWEISDDGLSYTFHLRKDVKFHSNKQ
                     FTPTRTLNADDVVFSFQRQMDLNHPYHKVSDGTYFYYHWMNLPKILKTVEKVDDYTVK
                     ITLQQPNAPFLTTLAMDFLSIYSKEYADKLLAEGKPELLDQAPIGTGPFVFQAYQTDQ
                     AVRYVANPDYFQGKAKFEKLIFSITPDAGTRYAKLKAGECDVIDFPNISDIAQMKQDP
                     KVNLLQRDGLNLAYIGLNSTKPALNNAKVRQALHYATDKKAIVEAVYQGGGTVASNPF
                     PDSVLGYNPNLPQYEFNLEKAKALLTEAGFADGFETEIWVQPVVRPSNPNPRRTAEII
                     QADWAKIGVKAKLVTHEWADFNKRTREGEFSTGTYGWTSRNGDPDNFLFPLLSKSNIP
                     GTNYGRWTDEKFEQLLAQAVQTQNRDERAKLYQQAVEIANEQTPFIPLAHAINYVPLS
                     KRVQGFVQNPFGYTPFYGVSLSE"
     gene            27713..28153
                     /gene="mioC"
                     /locus_tag="PXD81_000296"
     CDS             27713..28153
                     /gene="mioC"
                     /locus_tag="PXD81_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005605493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HDL5536365.1"
                     /translation="MTSICIITGSTLGGAEYVADHINEVLSEQGFEVELFNQATLADI
                     EGKTNLIIVTSTHGAGELPENIQQLFDDLANSSADFSSMKFGVIGLGSSDYDTFCNAV
                     NIVEEALKAKGASQVCESLRIDVMNNFDHDGSAEEWLPSFVEQL"
     gene            28165..28332
                     /locus_tag="PXD81_000297"
     CDS             28165..28332
                     /locus_tag="PXD81_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1232 domain-containing protein"
                     /protein_id="HDL5536366.1"
                     /translation="MNKISANKAKWIKIAIILIYMFSPVDILPEAILGPFGLVDDAAA
                     LMLLIKTILEK"
     gene            complement(28421..28864)
                     /gene="asnC"
                     /locus_tag="PXD81_000298"
     CDS             complement(28421..28864)
                     /gene="asnC"
                     /locus_tag="PXD81_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011609594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HDL5536367.1"
                     /translation="MDKLDQKILRWLIKDARTPYAEMAKVFGVSAATIHVRVEKMRQA
                     GIIESTKIVVDERKVGYDVCCFIGIILKSAKDYDKVIAKLSEFNEVVEAYYTTGNYSI
                     FIKVMTHTIEELHSVLAGKIQSIDEIQSTETLISLQNPISRDILP"
BASE COUNT         8459 a         6085 c         6130 g         8210 t
ORIGIN      
        1 tcagttaccg aatgatagtc atcaacttcc actgtggata aaaaaggagc aactcatggt
       61 gaaacaacat aaaatgtata ttaatggtga atttgtgcct gcgttagatg gtcgcacttt
      121 agcagtctat aaccctgcca ccgaaaaagc tatctccgaa gtccctcgtg gcggagctga
      181 tgatgccaaa atcgctatcg atgctgctga agccgcccaa gaagcttggg aacgtttacc
      241 agcgattgaa cgaggaaaat atttacgtca aattgctgtg gggattcgtg agcgtgccga
      301 tgaaattgct cgcactattt ccgaagaaat gggtaaaact ctgtccttag caaccgttga
      361 ggtacatttt accgctgact acttagacta tatggcagaa tgggcgagac gttatgaagg
      421 ggaaattatc caaagtgatc gcccaaatga acatattttc ctatacaaaa aagcgattgg
      481 cgtcaccacc ggcattttac cttggaactt cccgttcttc ctcattgccc gtaaagcggc
      541 tccggcatta gtaacaggta atactattgt gattaaaccg agtgaagatg cacctaataa
      601 tgcgattctc ttttcggaaa tcgtgcatca agtcggttta cctaaggggg tatttaatgt
      661 cgtcaccggc tatggcaatg aagtcgggcc ggaactttca ggcaatgaaa aaatcggtat
      721 ggtttccttt accggtagcc agtttgccgg acaaaaagtg atggaagccg ccagcaagaa
      781 tatcattaaa gtcaatttgg aattgggcgg aaaagcaccg gctatcgtat tagacgatgc
      841 agatcttgag cttgctgcta ccgctgtagt aaattctcga atcatcaatt caggacaggt
      901 ttgtaactgt gcagaacgca tttatgtaca gagttcaatt aaagagcaat ttgtccaaaa
      961 attggtagag aaaatgcaag cggtgaaata tggtaatcca ttagaacaac aagacattga
     1021 tatggggccg atggtaaatg aaaaaggctt gcataaagtg caggaaatgg tgacacacgc
     1081 actgcatcaa ggaggtaatc tggtattagg tggtaacgtg gttgaaggaa caggctacta
     1141 ctttgagcca actattatcg acaatgtaga taactctatg gatattatgc gtcacgaaat
     1201 ttttggtccg gtcattccgg ttgcgacatt tgataccatt gatgaagcga ttcgcttagc
     1261 caatgattgt gaatatggct taacctcttc ggtttacacg caaaatatca ataaagcgat
     1321 gaaagtaatt tcccgcttaa aattcggaga aacttatgta aaccgtgaaa actttgaagc
     1381 aatgcaaggc taccacgccg gttggcgtaa atcgggtatt ggcggtgctg acggtaagca
     1441 cggcttagag gaatacctac aaactcaggt ggtttatctg caatttgatg ataaagtata
     1501 gattcttcta aaaactccca cttatcaaaa taagcgggag ttttctttta ggttaacctt
     1561 gtatcaaaat tgcaaacaaa aatattctaa tgttaagtaa atgtttaata attatttatg
     1621 gttaactctt tttggctaat taatttgcaa tttaaattaa ttgagtgcag aatgccatta
     1681 gccttttttg aaatcacatt ccataaaatg gcttgattaa cacaacacaa ttatcactca
     1741 aattaggatt ttcattatga gtattttcca acaaaccaat ccaaaccgcc gtcgttatgg
     1801 gctagctgta tttatcggga ttattgccgg tattatttca gcattcgtaa aatggggggc
     1861 agaacatcct ttcccacctc gtagccctat cgatctgttt accgctgctt gcccgcaacc
     1921 tgtgttagat gcaatgaatg ccgcagcaga ccaagtggct gccaaaacca tcgcattaca
     1981 agaatgttca cgtgcatttt taaacccacc acacgttttc ttacgtgatt atattggtat
     2041 cgatccaact gaagcagcat ttacctttgc tgaccacgcg ttcaactgga ttggtgtcac
     2101 ccatatgatt ttctcgttag tctttgcgat tggttactgc attgtggcgg aaatcttccc
     2161 aaaaattaaa ttctggcaag gtatcggtgc cggtatcatc gctaatattt gtgttcacta
     2221 catcaccttc ccggcattag gtttaacacc accggttgct gaatggccgt tatatgaaca
     2281 catttctgaa ttagttggtc atattttctg gttctggacc attgaggtga ttcgtcgcga
     2341 tttacgtaac cgtattacgc gtgaacctga tgccgaagtg ccgttagatc aaccgttccg
     2401 ttaatttaaa attagctcga tttatacaaa agccgcaatc acgttgcggc tttcttattt
     2461 tacaagcggt caaattttca tgaaaattta ccaaacaaaa agcacattgt ttcatgtagc
     2521 aatgcggagc ttggctttat aagaaaaata caccaagttg gaacagtttt tcaatttcat
     2581 cgatctgttt cttatcgttc acaaaaataa tcacacggtc attttctgca atcatggtgg
     2641 atttgtgggc gataataact tcttcgcctc gcaccaccgc cccaacaatt gccccttgcg
     2701 gtagttttaa ttcacggatt tgtcgtccaa ccacttttga agtggttgca tcgccgtgag
     2761 cgataatttc taccgctcct gcaaccccaa gtttgagtga agaaacttgt aaaatatcgc
     2821 cttttctaac gtaactagcg agagcagaaa tagtagcttg ttgtggggaa atggcaatat
     2881 caatagtgcc accttgaatc aggtgtaaat aggcagggcg ttgcactaaa ataatcactt
     2941 ttttcgcccc aagccgtttt gccagcaggg cggacataat attcgcttca tcatcactgg
     3001 tgacggcaag gaataaatca atattctcaa tatgctcctc aaacagcaac tcttcatcgg
     3061 aggcatcacc ggtaagcacc atcgttttag atagtttttc tgctaatttt tcggctcgaa
     3121 ccggattgcg ttcgataatt tttacccgat actgatcttc taaatcccga gctaacgaag
     3181 agccgatgct ccctccaccg acaatcataa tccgcttatg tgggcgatca agccgttgta
     3241 gttcactcat tactgctcga atatgttggg ttgcacagat gaaaaagact tcatcacctg
     3301 cctcaataat agttgaacct tgtggcacaa tcgctttgtc ttggcggaaa attgccacga
     3361 cccgagcatc aatatgcggt aaatgatcac gcagggctga aatcgggtaa ccgaccaaag
     3421 gcccaccgta ataggctttt acattcacga tactgactaa ctcatcagca aaatgagtaa
     3481 tttgcaacgc ccccggatag tgaataaggc gtaaaatatc ttctttgact aaaatttcag
     3541 gggcaataat atggtcaata ggcaatacat catcattaaa caaccgctct cgttctctca
     3601 cataatcagc actgcgaata cgggcaattt tggtcggcac attaaataaa gtgtaagcaa
     3661 tttggcaagc gatcatattc acttcatcac tattagttac agcaactaat agatcagcat
     3721 cacctgcacc ggcatcacgc aaggtttgag gtgaagcata ttcgccccga atcacctgta
     3781 aatcgtgttt atctttgagc ttgtctaaac gagttgggtc attatccacc aagacaatat
     3841 cattgtcttc actcaccaaa ttttctgcca gcgtagaacc aacttgtcct gcccctaaaa
     3901 taattacttt cattactctg ccactttttg tagttttgcg tagaagaaac catcaccacc
     3961 gtttggttgt gggaagaact gtttttctaa cactttttcg ccgttaaaat cgatttcaag
     4021 ttgttttgca ttcggtgtat cttgtaaaaa ccgttgaatt tgctcgctat tttcttgcgg
     4081 taacaccgag caagtggcat ataacaaaat gccgtttggc tttaaccttt gccataacgc
     4141 cttgagaatc tcaccctgta atgtggctaa ttctgcaata tcaccctctt tacgcaacca
     4201 tttaatatca gggtggcggc gaatgactcc ggttgcggaa caaggggcat caagtaaaat
     4261 gcggtcaaac agcacaccct cttcaagcca ttcctcaggt ttggaagcat cgccacaaat
     4321 tacctgtgca atctgcccca gtcggactaa gttttcacgc actcggctta aacggctctc
     4381 ttcaatatcc aacgccataa cctttgcttt cggtgctttt tctaaaatat gggtagtttt
     4441 gccacccggt gcggcacagg catctaaaac ggtttcacca ttttctgccc cgagcaactc
     4501 cgctgaccat tgtgcgtgag catcttgcac ggttgcccaa ccttgctcga aatgtggcag
     4561 ttggttcaca tttaccgctc tctccagcaa taaagcacac tttggcacgc aagcggtcgg
     4621 attttctgaa ttttttgcaa attcaggctc aaccagctcg ctaatcaacg ccgcataatc
     4681 attggtcttg atatgttgtt gattgactcg aatccacatt ggtgggcgtt ggttgtttgc
     4741 ttcaacaatt tctcgccagt tcggataggc ttttttaagt ttattgacca accattcagg
     4801 gtggagcgtt tgccaatgtt tatctacctt cgctaagatt tcttcttgct ctcgcagaaa
     4861 acggcgtagc acaccgttag tcaaggctcg gaagctatcc agttttagcg ttttagttgc
     4921 attcactacc tcatcaaccg cagcgtgtgc cggcactcgc atataaagca gttggtataa
     4981 acccactaac agtaagcaat gcaccaaacg ggttttgcct ttcagcggtt tttcaactaa
     5041 taatttgatg atcgattcca agcgaggcag cacacggcac actccgaagg tgatttcttg
     5101 cactaagggt aaatcttttg gttcaagctg tttttgagcg tcaggaatca aggtggaaag
     5161 tgatttacct tgatctaaca cctgtaaaat aatggttgca cttattgctc tagaagaaag
     5221 tgctttgaac gattgttttt tcataaataa aaatgccatt ccgacagaat ggcactattg
     5281 taactgaatg cgggattatc ttaaaactaa aattggcttg gtggtgttgc gagccacttt
     5341 aaccgtatta gagcttaagc ccttcgcatt tggtttgctg cttgccagca tcataattaa
     5401 atccaccgac ttttcatcgg caatacggca aatttcttca taaatcgtac catacgccac
     5461 aatgtgctgc acttttgaac cttcaggaaa atggtttttg gtaaattcgt gcaatttttg
     5521 gtttgcttca gccaccactt ctttatcgaa atttttcggc aagaatgcag aaattaagct
     5581 gttatccacc ggctcaataa tagacacaac ccgataaacc gcattcggat tttttgccgt
     5641 catttgtaat gccgtatcta ccacatattt agcacttttt aggtcggcta aatcaatcgc
     5701 aataagtaat ttgttataca tatttattcc gtaggggcag gtcctgtgcc tgcccgaata
     5761 ttaactaaaa tagcggacgg gcacaggacc cgtccctaca tagcatataa atactatgca
     5821 ataacacact gttttttcac tctacggcgt tggtttgcca caataccaaa taccaacaat
     5881 agagcaggaa tgaagatcca atctttagaa ggagcatctt gcggtacggc aatgtagtga
     5941 atggtttgat cccagtttaa gcctgcttta gctgccggag aatccatttc taccatatca
     6001 atcaccactt ttggtgtttc tacgccatca actttgattt tttcatcggt ttgaattagc
     6061 gttaagccta agtttttcaa acgctcttcg ccggttgctc cttccggtac aggtaattga
     6121 gcgtagaact caatttcttt accgtaaggg tttaatccct ctacttttaa agtaacattt
     6181 tgacctactt tgcttgcaag caactgttct tcaaactgca ccggctcgat atgacgttca
     6241 gacggataaa ttctgtccat aaagaagccc ggacggaaca aggcgaaagc ggctacaacc
     6301 agcaacgccg tttcccacca tttgttttta gtcacaatat attgcattgt agcagaggta
     6361 aaggcgagaa ccccaacagt tgcggtaata aacacaaccc agccgtgccc cggataacgc
     6421 agatcaatta ataataaatc ggtattaaaa ataaataaga acggtaaaat gacggttctt
     6481 aaactatatt taaatgccac cataccggta ttaatcgggc taccgcctga aattgctgcc
     6541 gcagcgaacg aggctaaacc aacaggtgga gtcacatctg ccataatccc aaaatagaat
     6601 acgaataaat gcaccgcaat caacggcacg attaacccgt tttgacgacc gacttccatg
     6661 ataaccgttg ccattaatga tgacaccaca atgtagtttg cagttgtcgg taagcccatt
     6721 cccaaaatta agctgaatac ggctaccaag atcaacatta gcacaatgtt gcccattgat
     6781 aggagttcaa tcacacttga aagctgaacc ccaaagcccg ttagtgccac aacgccgaca
     6841 ataatccctg ctgttgcggt agcgagggcg ataccggtca tattacgacc gccagcctca
     6901 aggctatcta ccacatctcg aacaccttgt ttgataatct ctttggttac gctctcttta
     6961 cggaagaaat tcagtaacgg gcgttgggtt aattgaataa aaattaaggt aatggttccc
     7021 cagaatgcgg ataagcccgg cgaaagcatt tccaccatta agcaccaaag tagcacaacc
     7081 acaggaataa ggtaatgtaa acctgcattt acggttggtt ttctttttgg tagagaaaca
     7141 atttctgagt ttggatcgtc cgcttctaaa tcagggtagc ttgccacacg gcgaatagcc
     7201 agtagatacc ataagcctaa gaaagcacag acaatcataa acgcataatc ttctgccaca
     7261 tcacgaatcc aacctaagcc gtagtaaacg ccaatcgcta atcctacgat cactaaaatt
     7321 gaagctacgg ttcttagcaa gaagactaaa atcgggcttg gctcttcggc tcttggtaag
     7381 ccttgtaaat tcatcttaca agcttctaaa tgcacgatat acaccaacgc aatatacgaa
     7441 attaacgcag gtaagaaggc gtgagtaatc agctggctat acggcatatt cacatattcg
     7501 atcatcaaga acgctgcagc ccccattacc ggtggcataa tttgaccgtt tactgatgag
     7561 gcaacttcca ccgcaccggc tttttctttg gtaaagccga cccgtttcat cattggaata
     7621 gtgaatgtac ctgtcgtcac gacgtttgca atcgaagagc ctgaaatcaa gccggttaaa
     7681 gcggaagaaa ccaccgccgc ttttgccgga ccaccacgca aatgccctaa ataggcaaag
     7741 gcggttttaa taaagtagtt acctgctcct gctttatcga gcaacgcccc aaataatacg
     7801 aacaggaaca catatttggt cgaaacccca agggctacac caaacacgcc ttcagtggta
     7861 atccattgtt ggttgataat ttgagaaagc gaacctgagc ggtggcttac aatccaatct
     7921 gccgggaaat attgcccgaa gaagttatag atcaggaata caatcgcaat aatcacaagc
     7981 ggtaagccaa gactacggcg ggtcgcttcc agcaccaata aaatccctaa acagccggcg
     8041 ataatatctt gcgtatttgg cgctccaaaa cgtttcacca aaccttcata gaagaaaata
     8101 taatacaacg ctaacccagc tcctacgatg gcaaacaccc aatctatttt aggaatatga
     8161 tgtttaggcg agctggcaaa agcagggtaa gataggaacg ccaagaacat tgcaaaggct
     8221 aagtggatag aacgggcttt ggtgtcgtcc accaccacat tcaaattaat gcctgcgtga
     8281 gtaagccatt cttggaatac aaacggcaat ggagaagcat aataaatttg gaacacagac
     8341 caaagaatgg cgacatacac aatggctttt ttcgcaagac cggacggggt tcgcccccca
     8401 ctgtcattgg aggcaaccat atcttgtagg tcatcgtaat caaccttaga ttcaactgac
     8461 ataaagactc ctcaaaacgc aagcggtcat tttcttatta aattttacaa atttcctcaa
     8521 aaaacaaccg cttgctcgtg aaaacactgt tgataagtta tcaattaaag gtgaaaatcg
     8581 ataacttatc aacagtttca aaaaagcaaa aaaccagcac gctaaaaaac gtgctgattg
     8641 aatcaaatta cagaaaggaa atattactta atccaacctt tctctttata gtaacgaatt
     8701 gcaccttcgt gtaacggagc agaaagggcg tttttgatca tctcttcttg ttttaagtgt
     8761 tcaaacgccg gatgtaaacg tttgaaacgg tcgaagttat caaataccgc tttggttaca
     8821 ttgtacactt tatctgcatc cacatcggca gaagtcacta acgttgcata tacaccgaag
     8881 gtttctgtcg gctcatccga acctttatat aaaccgcccg gaatcgtagc tttggcataa
     8941 tatgagtgat ctgccactaa tttatcaatt tcagggccgg ttaccggcac taatttcgca
     9001 tcgcaagaag cggctgcttc ttttaacgca ccattcgggt gacctacgtt ataagtgatg
     9061 gcatctaagt tgttatcaca cattacagat gccatttctg ccggtttcaa ttcagaagcc
     9121 actttgaaat ctttatccgt ccagcctttt gccgctaaaa tcacgttcat tgttgcacga
     9181 gtaccggaac ccgggtcgcc cacgtttaca cgttttgctt ttaaatcatc gaacgattta
     9241 atgcctgaat cggtacgagc cattactgta aacggttccg ggtgaagcga gaaaatggca
     9301 cgaattttat cgtttttctt accttcaaat gagcttgagc cgttataggc gtggtattgc
     9361 cagtctgatt gtgcaatccc catatccatt tcttttgctg caatcgcatt taagtttgca
     9421 accgatgccc ctgttgaagg agcgttacat tttaaacctg tttttgctga atcacggttt
     9481 actaattggc aaatagattg acccacaaca taataaacac cggtttgacc gcctgttccg
     9541 atagtcacaa atgtttcagc cgcttgaaca gatgctgttc ccatcacaag gcttgcaagg
     9601 aaagaaagtt tgactaattt tttcattttt aactcctaaa attaaggatc agaaaacaaa
     9661 gatttattgt tttgagccac tgctcacact acaagagata caaaacttct ttgagttgca
     9721 tttgcctctt agagcttgtt tttagcatag attttataat tagtaaattt attttttcgc
     9781 ataaaaaaat ctatgcctaa tgccacaagc taaaatgttt cactatctct taacaaatgc
     9841 acatcgcctt ttcgtcttaa aaaaccgcta cgatcaaaat attcaattaa ttgtaccgtc
     9901 aattttcgcc catactgcaa ctcatctcgc aactggttca ccgaaatgct gccgttttgg
     9961 gcgataaaat ctttaattaa cgttgcaaat tgcccgattt gttcgcttaa taaaaatcta
    10021 tctttgacga ttgggattaa atagcccaat ttcccgactt tatacagcaa attccgcatt
    10081 tgggtttcat caatcgagag ctggcttgca atatccctca cccaaagagc ttgattggtt
    10141 gcctcaaaca gcggtcgaat ttgttgccaa atttgcaatt catcggcgtt caattcgatt
    10201 cgatgttccg gcaaatgtaa ccaaccacga ctattgacca aggctttttg ctccaccaaa
    10261 tcctcaataa attgatttgc caagttttca ggcaaatcca ataacgctat gcgatataaa
    10321 cgagctttgg tcacgccgat ttgatcttga tgttgcaggt ggtattctgc cattttttcc
    10381 aacacccgtt gctgggcttg agctttaaaa tccgaattaa ataaccattt agacgagaca
    10441 tcaaacccca gttttgcaat atcctctgca aaacattgct ccgcccataa cagcaaggat
    10501 aaatccatcg ctctgttttg cagatagaga tcaaggcgtg tcgcaaagtc attacaagcg
    10561 gtggtttttg ctaaattttt taccaaagct aaacgggctt cggtacgttt atgccgtttt
    10621 ggtgagtgaa tttccagcac ctccgctccg gctaacgtta ggctgtcatc accgctgcgg
    10681 atgatcaact tatcaccaac ggcaatatga agtggttcat ctaaaatcac ttctgcaaaa
    10741 aattgctgat ttttgaccgc ttgttgcccc tcaagcaaat tcaatttgcc ggtaatgtgc
    10801 gaagcaaaat ggtaaagatg cacaatgctg ttttctttga aggcttgatt ggcagtgagt
    10861 tgaaccgtaa tgcgatcggt ggcaaatttc ggctcaagct cggtaatcca atcgcctcgc
    10921 ttgatttcct ctttttccac gttagcgata tttaacgcca aacgttgccc tgccactccc
    10981 gtttccgaag gcgtattttg ggcgtgaatc gctttgaccc gcactttttt accattcgac
    11041 aaaaataact cagagccaac gctcactttt cccgccaccg ttgtgccggt aacgaccaac
    11101 cctgctcctt tcacattaaa cacacggtca atcgcataac ggaacggttt atgggtttgg
    11161 gtatgttgtt gatttaattg aattaaatgt gcttttaatt cttcaatccc ttgttgcgtt
    11221 tttgccgaag taacaaaggt ttttgcttcc gctaaaaacg gatagcttgt tttcacttgc
    11281 tcaattaagt ggctgatttg agtttcttcc gcccgatctg ccttagtaat cacaatcata
    11341 atatgctgga aattcagcag gtggagaata tccaaatgct cttcggtttg tggcttaatc
    11401 ccttcttcgg cagaaatcac caacagggcg tgttgaatgc cgccaagccc tgccagcata
    11461 ttagataaaa aacgctgatg acccggcaca tcaataaacc ctaaaatatc gttctcaatc
    11521 ggtaaatagg catagcccaa atcaatggtt agcccacgtt tttgttcttc gggcaaatgg
    11581 gcggtgttag tgccggttaa ggcttgtaaa agcgatgttt tgccgtggtc aacgtgaccg
    11641 gcggtgcaga tgatcattga tagtccttat aaaaatgagt aactattctg gcgagcagat
    11701 ttaaaatcta caatatagac attttcctga ccaatcagga actttacttg gtacttaccg
    11761 tttaaaatgg gaatttcttt tttcttgcga agtcgataaa catcggcagt aaaggaaagt
    11821 ttatccactt cactttttat tgcatcataa aattgttctg caataatcgg gttttgggat
    11881 tgttgtaata tataatctgc ttgataaaac aattttattt ctgctttctg tagccaaaca
    11941 atattgtaat caaacttcat catttttccg tgattcccaa ttttttgcgt aaatcttcca
    12001 tcacctgagc gtgggattta gtttcaatta agccattttc taattgatgt aataaatcaa
    12061 gttcatcatt agtataaaaa ctttgtggct tatctgtgcg taattttgct aaatcttgtg
    12121 cgagtatgag ttccatattt cccccttaca aacttagatt attcaatatt gtaacctgat
    12181 aaaactaaag ttcaaggagt gtatttaagg cgataaccgc tcttttttcc actcattatt
    12241 ttccaaccaa taatgaatgc ggtcgtgtag ccggctactt ctgccttgcc agagttcaat
    12301 actgtcggga atcagaatat agccacccca atgcggtggg cgaggcactg aaagtggata
    12361 tttcaatgca aattgagctg attttagcaa caaagccttg tagttttcga taggctgact
    12421 ctgctcgctc gcccaagccc ccaaacggct ggtgtaagga cgagagtcaa aataggcatc
    12481 ggattctgcc tctggaattt ttgctatttt cccttcaatc cgcacttgac gttgtaattc
    12541 tgcccaaaag aaggttaaac atccaaaagg attatcggca atttcccacc ctttacggct
    12601 ttgataattg gtaaagaaca caaagccttg tgaatttacc tctttcaaca gcaccattcg
    12661 gctacttggt cgcccgtttg ctgcaacggt agcaatattc attgctgtcg gctcattcac
    12721 tttggcattg attgcctcac tcaaccattg ttcaaattgg gtgattgggt ttgagtgaca
    12781 atctcggatt gaaagcactt ttttggtgta atcttctcga atgtggtgca gatccataag
    12841 gattccttta tcttattcta aataatcgga caggcacagt ctgccctacc aaaattaata
    12901 ttccaacatc tcaatcaact tatcaaactc cgcgaccgag cgtaaatcta accaaaattt
    12961 ctgctgagca aaacgcccga taatcgggct tgggtaggtt ttaaattgtt gttccaatgc
    13021 aattaaatcg ctctgttttt cagcggaaat agtcactgcc accgaaggta aggtttctgt
    13081 gggtaacgca ccgctgccga tttgagcgag gcttagctca atttgcaaaa tataagcaga
    13141 atttaaccgc ttgtgtagtg cctgttttaa tcgctctgct ttgtgttgca aggtttcaag
    13201 cggttgagtg agtaaatgaa gagtcggcaa gttatcggtt aattgctccg gaaataagta
    13261 gtggcgtagc gttgcctcaa gtgccgataa aatcacttta tcgcaacgta gcacccgttt
    13321 tagcgggtgt tgttgcagtt ggctaatcat cgcttggcta ccgacaataa tgccggcttg
    13381 cggcccgcct aataatttat cgccagagaa ggaaaccaaa tctaccccag ccgctacttt
    13441 ttgctgaacc tttggctcag caggcaaatt aagagcttgc atatcagtta gcgaaccact
    13501 gcctaagtcg gtaatcacag gcaggttaaa ctctttaccg agagccacca attcctcttc
    13561 cgacaccgaa ctggtgaacc cctcaatgtg gtagttactg gtgtgaactt tcattaaaaa
    13621 ggcggtattt tcggtgatcg cgttgcgata atcctttaaa tgagtgcgat tagtcgtacc
    13681 aacttccacc aatttgcaac cggcttgagc cataatatcc ggaatgcgga atgccccgcc
    13741 aatttccacc agctcgccac gggaaacgat gacttccttg ccttgtgcga aggttgctaa
    13801 catcaacaac accgctgccg cattattatt cacaatgcaa gcggcctccg ctccggtaag
    13861 tatttgcaac aactcggaaa tgtagttatc tcggtgcgag cgcttgcctg cttcaatatc
    13921 aaattccaac gccacattgt ttcgcattgc gttggttgct gcctgtatcg ccttttccga
    13981 ccacaaccca cgccctaagt tggtgtgtaa caccgtaccg gttaagttga acacggaacg
    14041 gattttcacc tcactcaacg cttgcacttt ttgttccagc cttataaaaa acaggttttc
    14101 atcggcaata aaatccggca aggcttgctg ttgggaaata aattgacgtg cttgagtaag
    14161 caaactccta gcttgttcag ccacgctgga atatgcaaat ttttggcata attcgaccgc
    14221 ttgtggcttt ttcaacaaga ggtcgagcga gggaatttgg cgaaatagtt gttgcataat
    14281 gtgaatgatt gagagaatat tttggacaat tctaataaat aaagtacaat attgccaatt
    14341 tttcggaagt gcgtcatctc cggtgaggtg gcaggacttc aaatcctgtt gaggacgcca
    14401 gcgttctcgg gtgggttcaa ctcccatgca cttccgccac atttcaaaag ccgttaatct
    14461 ttaacggctt tttgcatttc tgccacattc tctctttccg ttttgataaa aaatgagact
    14521 tagctcacat ttttttattt ttctgtttgt agtaaaaatt tattaaatta taattatttg
    14581 gagtattttt tcacaaacaa aatctagtcc attgactacc ttttgaacat aaaaaaggaa
    14641 ctgttatgtc aacactatct cgtgattcta ttttaagtca aattcaaaac gggctgattg
    14701 cctcctgcca accggttgat gacggcccca tggataaacc tgaaattgtc gccgcaatgg
    14761 cagcagcctc tgtgattggt ggtgctaaag gcattcgcat tgaaggtgtc gataatttaa
    14821 aagccgctcg tgcggcagtg aatgtgccga ttatcggcat tgtaaaacgt gatttgccag
    14881 attcccctgt gcgtattacg ccatttttag aagatattga tgccttagcc gaagccggtg
    14941 ccgatattat tgcggtggac ggcacattcc gcacccgccc ggtcagcatt gaagaggcgg
    15001 taaaacgcat tcacgaaaaa ggttgcttgg caatggcgga ttgctccacc cttgaagaag
    15061 gtttacattg ccaacaatta ggctttgata ttgtgggtag tacaatgtct ggctacaccg
    15121 gtggcgaaat gcctgatgag ccggactatc aattagtgaa agatttaaaa gctgccggct
    15181 gtaatgttat ggcggaaggt cgctacaaca cgcctgaact ggcaaaaacg gcgattgaaa
    15241 tcggggcgga ttacgttacc gttggatcgg cattgacccg cttagagcat attgtgagct
    15301 ggtttgccga ctctgtaaaa tcggctcaaa aataggagct gcttatgcgt tgtttagcaa
    15361 tagacatcgg tggcacgaaa attgccacgg caattgtgca acacaatcaa gtgtcacaac
    15421 gccaacaaat tcagaccccg actgataaac cacaagctga tcaggcaggc agtttgcatc
    15481 aagcgattgg tgagataatg caacattatc aagggcaatt tgattttgtg gcagttgcct
    15541 caacaggcat tatcaataaa ggggtgctta ccgcattaaa tcctaaaaat ttaggcggat
    15601 tggcagaatt tccgttgaaa caaagcattg aaaaacatac cgataagccg attgcattat
    15661 taaatgacgt gcaagcggcg gtgtgtgccg aatatttaaa tgaagaccaa caagcggtca
    15721 aaaattttgt atttattacc gtttccaccg gcgtaggcgg cggcattatt ttaaacggcg
    15781 aactgcaaat cggcagtaac ggcattgcag gacatatcgg gcatactcta gccgatccga
    15841 acgggcctct ctgtggttgt ggacgagtcg gctgtgtcga agcagtcgca gcagggcgtg
    15901 caatagaggc agtttccagc caatgggaaa agccttgttc accgaaagag gtgttcgagc
    15961 gattccgcca aggcaaccca caagcggtcg aattagtaca aaaatctgca aaagcgattg
    16021 ctaatttaat tgcagattta aaaatcagtt tagatataca aaaagtggtg ttaggtggca
    16081 gtgtgggctt agcggaaggg tatttaccgc aggtgtccaa ctatttagct gaaatgccga
    16141 gcgtatatca ttgcgtattg gaaaatgctc tatccgggca agatgccggc ttaatcggtg
    16201 cggcttggtg ggcggaaaac caacttaatc aaggttttac tctttaacaa attctaactc
    16261 cggagatcca aatgaaaaac ttaaaaggta tttttagtgc gttactcgtc tctttcaacg
    16321 aagacggcac catcaacgaa aaaggcttac gccaaattat ccgccacaac atcgacaaaa
    16381 tgaaagtgga cggtttatat gtcggcggtt caaccggtga aaactttatg ctttctaccg
    16441 aagagaaaaa agaaattttc cgcattgcca aagacgaagc gaaagatgaa atcgccctta
    16501 tcgcccaagt cggtagcgta aacttaaaag aggcggtaga actgggtaaa tatgccacag
    16561 aattaggcta cgacagctta tctgcggtca caccgttcta ctacaaattc agcttcccgg
    16621 aaatcaaaaa tttctacgaa accattattc gtgaaaccgg caacaaaatg attgtttact
    16681 ccattccgtt cttaaccggc gtaaacatcg gtgtgagcca attcgctgaa ttatttgaaa
    16741 acgaaaaaat catcggtgta aaattcactg ccggcgattt ctacctgtta gagcgtttac
    16801 gcaaagcgtt cccgaaccac ttaatctacg caggtttcga tgaaatgatg ctaccggcaa
    16861 cggtattagg tgtggacggt gcaatcggct caaccttcaa cgtaaacggt attcgtgcaa
    16921 gacaaatctt tgaattagcc caacaaggca aaatcaaaga agcatttgaa atccaaaatg
    16981 taaccaacga tttaatcgaa ggcattttag gcaacggttt ataccaaacc atcaaaggct
    17041 tattggagtt agaaggtgtg caagcaggtt actgccgtga gccgatgacg aaagaacaaa
    17101 ctccggcaca aaaagaagtg accaaacagt taaaagctaa attcttgtaa tttaagttgc
    17161 aaaaggctaa acgaaaatca ccgcttgtag taacaagcgg tgattttttc tcaacgactt
    17221 gcaattaatg aagccattct gctttttcta ccacggtgta acgtgtcacc attatatttt
    17281 aatcgcactt tttcttggcg agtattttct gatttcaact ctctacctat atcataaaac
    17341 cctgtactcc agatattttg agtaatatcc gcttttacaa taatatcatt gaagccgttg
    17401 gtctgtttgt tatccatttg caataccaag tttgtgtttc cgttttggtt tctttatcat
    17461 ctaaaataga ccaaaaagtc atcgggtaat caaaaacaag ttttaacttt tctttttccg
    17521 gcacaaataa catcaaatga tcatttgcag aatactcagg agcacttgga ccacgagcag
    17581 cattattaaa cctcacgccg aaagctctta ctttgtcgtt tagaatataa cgggcggtat
    17641 caattttaag actatctgca ttcacatcga tagaggcatc gatctccata tcctgctccc
    17701 acgattgcaa tatcgcatta tcattcacac gagcaacaaa aagcgtccac ttaggtgtgt
    17761 tttcgtcgtt ttgataattt tctttgctgt atttaggctc tattaccgca taactcacta
    17821 aggtttccgt tgaattgtta ggagctgttt tacaggcaga agctaaagta cgagcagaaa
    17881 gtttctcttg agaaatctta aagtaatgag caataatttg atgttgttgc tcagcacaaa
    17941 cctgtgtgtt attaccttca tctgcttgag ttgcaagcgt aagtaaaaga actaagagga
    18001 gtatcttttt catatagatt tatccgtata gaaagataac tcattctatg gggtactgac
    18061 tctttttgca tagagagaaa gaaaaaaatt tggtcggaaa aataacggcg agcctagcga
    18121 aaacaagggt tcgccgtaat tttatggggg aagctatgta aaaaacaaaa tgagcaggat
    18181 tagaaaaaaa tgagcagttt ttgcgatatt ttttgcatag gtttgatcgg ttttaaaact
    18241 attttaaatc tgttttaaac taaagagtgt ccgatgtgga ctacctgccc gatgatttgg
    18301 aggttttgag cttcttcggc ggtaatctgt attgggcggt aggactcgtt atcgctaatt
    18361 aattcaatat agtcccattc ttttctaatc cgtttaaccc atacgctttc cccattctgc
    18421 acaagataaa tcttatcatc accacgaggc tctcgtttgc tgaaatctac cagcatttgg
    18481 tcactgtctg caatggtagg gtgcattgat gttccatttg cccaaaacac gccgcagttg
    18541 tgggctttta cgccgaggct ttgcagcagg ctgtcggcgt aaggttcttg cccgtctggc
    18601 ttcgttacgc cctcgttaaa gctgccaaag cctgctgaaa cgttgattgt tctgaagctg
    18661 ttaatcattg aaacaatatc atcagccatt ttttctagtc gctcaaactc tcttgccaca
    18721 atactggtat tctctgattg tggtttgctt tccttacctg ttgccagcca ttcaaggctc
    18781 acatttgaca cctctgctat ttttacaaga tttgaacgag atggatctgc ctctcctttt
    18841 acccacctag atagtgatgc ttgggctacc cccactgctc ttgcaaactc actgtaattg
    18901 tccttaaagc tattttttgc aatccatttc attctttctg agaagctctc atcgtaaatg
    18961 tttggtttac tcatctttta cgtttccatt aataaagttt tactcgcaaa acatactcat
    19021 aaaccattga tttttaagtt atttatgaat aaagcaaaaa tattttagat gtttttatgt
    19081 tttacggttg atttgtttaa agttttacgg ttaaatacac gcatcaaata gataaaaaga
    19141 ggtgtatcga atgagtgtat taaacgatgt taaaaaaacc gcacagagag attggcataa
    19201 agccgatatt cttgcggaac ttcgtaaaaa aggttggtca cttcgttctt tggctaaagc
    19261 aggcaatgtt agccataaca cgctgaagtc tgttttagac aagccttacc ccaaaatgga
    19321 acgcttagtt gcaaatgcca taggggttgc ccctgaagtg atttgggctg ggcgaacagc
    19381 agaacgcatt gaacgtaatc ggcgaccaac tttaactaat aagttttaat cataaatgat
    19441 ctttaacgta aaagatatag gagaagaaca tttatgagcg aattaagttt aaaaacgcac
    19501 tattcagtgg ctgaattgtt aagttttaag ttatctagcc taccaagtgc tcataaaaat
    19561 gtgcttgaga aagcaataag ggaaaactgg caatctcaaa aacgcaaagg cagaggcggc
    19621 ggggttgaat atgaattaat cagtttaccc accgaagtcc aacaggaaat ccgcaccaag
    19681 ttactcaaac ttcttcctgc tgaaatcagc aaaggcgaat tgagtgtagt gcgacaaaac
    19741 attgatttgg agcagataac cgataaacaa ctttctacgg cagatgctcg cattatggtg
    19801 gtgcggtggt tcttgatgca ggaagtgcag ttggggctaa gccgcacaaa aacgctagac
    19861 caagtcattg cagcggtggc aagcagtgaa attcctgctg aaatttgcaa agcgattatg
    19921 gcgggcaata gtaaagccgg tggaaagtta aagctatcta aacgcacctt gcatagttgg
    19981 gtgctggcgt atgaggcagg cgaaaattca gcggaacggt taaaacagat gataccgctg
    20041 aaaacacaaa aacgtgccgt gcccgagcgg tgtggttggt tgcaagcctt tctgccgttt
    20101 tatcaaacct ttagcaatgt ggcactcaca caggcttatg ctcaatttgc aatgcagtat
    20161 gagggcgaag atttacctac agagagccaa gttcggtatg ccttgaagca gttgcccgat
    20221 tatgtggtgc aacaagggcg gaaattgccg gtgaagaaga tccggctcgc ccgttaaata
    20281 cccttgaagt taaaggctta acggtcgtag atgactcgca gaaaccgtta tttagcgaag
    20341 taaaccaagc gttatatcac ggtttaagcc cgattgaggt ggtggcaagc cgtgtgcaga
    20401 ttacccgtgc gattactaca tacaccaaaa acgtgacgaa tactgatgac ccaagctatt
    20461 tggatttaac caccattcgt acgcttgact atgtgcgtaa ggcaatccaa acccgtcagc
    20521 gtttgcgttt ccctcgtgcg aaaaactccc accgtattgt ggcaaaagta cgctcggaga
    20581 ttttagatgt gctttatcag cttgagggcg aagaggtaat cgaaaatgtg gaagcgtgga
    20641 aaaatcgctt attagtggtg cgtaatcaag acccgactta cttggattta gaaatccctg
    20701 cggatgtggt taatggcttg cacgttattc gtaacaaaat tacgttaatt ttataggggg
    20761 tgctagatgt caattgttta tgaaggctct tgcgtgcttg aggtagatgg cgttgagatt
    20821 gaaattacca aacttgatgt aaaaaccgta accggtcgca agctggttaa aacgatgaac
    20881 agttccggac gtgcaagagg ttattcgcaa ggcattgcgg aatatactct ctcggtgact
    20941 gcagttgagc ctcaagacgg ttcggtaatt gattgggata acattaaaaa tgcgaagctc
    21001 actaaatacc cgttagacaa tagcgaaaaa cgcacctctt atttaggctg ttttactacc
    21061 gatgtgagcg agtcttacac cgttgatggt gagtcggtgc gtgatattca actcaacgca
    21121 ttgcgtaagg tgaatgaata atgaaaatca ctttaaatct tggcattgaa tataacggga
    21181 aaattcgccg tgattgtacg gtgcgattgc tgacgatggg cggtcaatgc acggctcaag
    21241 aaatgattgc ggatttaggg tttgaggaaa actcggaagc cctatcaaag cgtgaacaaa
    21301 ccttggtcga tatggcttac ctttcgcagc aagttacctt tgatggtatt tatcaaggta
    21361 atgtgacggc tcaattttta tttgacaatt tagctaccga agactactgg cagttgattg
    21421 aggcaacgct cacactccga aaaaagcggt tagaaaatgg ggtgagccaa agcgagagcg
    21481 aaagcccggc aaatcccgat ttggcgtagc agaagccttt aagcagtacc gacaagcggt
    21541 gattttactc tcaaaatttg caattaccgc cgagcaggta tggcaaatgc cgttgcttga
    21601 ggtatttgcg tggattgaga gctacttgga gagtgagggc gtaaaaacac cgcatagccc
    21661 aatgggtgcg aatagcgaag tgaaacgcga aagctatgtc tttactcggt gtggcaaaat
    21721 tgaatagggg cgaaagcccc ttttttgtac tttaaaatta gtttaaagag actttaaaaa
    21781 tggcaaaaaa tatgaaagtg caactggaat tgacggcaaa agatactgcc tccccagttt
    21841 taaaccaagc cgctaaaacc gccgaaaaag cctttaaaaa tacagagcga gcggcacaaa
    21901 aaagtggcaa tgctcaaacg caagcagcac agaaagcagc gaatgctaca gaaagctcaa
    21961 atcggaaaat agaacagagt taccgtaatg cccgccgagt ggctgccgat gtgagccgag
    22021 cccgtgaaac attaggtgtt cgggcggaaa atgcaatcca acgcgaaatt gaacaaactc
    22081 gtgcagccta tgaccgctta aaacgctcag gtgttgcctc tcaaaatgag ctacgccgtg
    22141 cctcagaagc cactaagcaa cgggtgcgag agctgaatgc agaattgggt aagacaagct
    22201 ttggggataa ggctaaagga tttgggcgtg gtttgatgag tgccggtgtc ggtattgcag
    22261 caggtgctgc aatggctcgt cagccagcca agaaccaaat ggagtttgac cgacagttag
    22321 cgatggcatc aaacactgcc ttttccgacc gtgatgttaa agggcgtatt gagggcaaga
    22381 aagagttatt agcagcggtg caagaggcgg ttaaggttgg gggtggaaca aaagaagatg
    22441 ctcttttaac tcttgatacg ctaattgcct caggaacgat gaaggctgaa actgcaatga
    22501 gcctattacc aaccttgcag aaaggtgctg tagcaacagg tgcaagctcc gaggatatgg
    22561 caaaaattgc gatctcctca atgcaacaat ttgggattaa agaagaggat attgggcggg
    22621 cattggatat ggcagttgca gccgggcaag caggtaactt tgagcttgca gatatggctc
    22681 gctggctacc acaacaaatg gccgcaggta acacggcaag gcattgggcg gtttattacc
    22741 gcctgtatcg tatgacccaa acggcagatg gctagcgtta tcccttgaag ttgaggggag
    22801 agagctagac cgcattgatg cgagagccaa gactttaatc gatgccgttg atgtatcaac
    22861 aggcattttt attgatgatt gggagcgggt ttgtggctta acggcgaata gctcactacc
    22921 gcttgaaacc cgaattgagc gtgtgattgc aaagctgaat cagttgggtg gattgtcgat
    22981 aggctatatc actcaagtgg ccaaagagct tggatattgc atcaaaatta gtgagccaca
    23041 accctttatt gctgggctaa gccgagctgg ggatatgtta tggcataaag acattatgtg
    23101 gacatttttt gtggatgttt gcaaccccac agatgattac aagcgtttta gagcaagcgt
    23161 gtcaatggct ggcgatgcgt taatgggacg atacaaagac ccaattttag aaagtctact
    23221 tgaagaggta aaacctgcat ttagccgtgt gtgggtaaga tatttaggag atattgaatg
    23281 aaacaagtga tgaatccgat taatacccca acacagcgtt ttaaagatgg caaccctgcc
    23341 actggtgaat atggcacgat tgtgactgct gaatttttga ataacgtcca agattcggtc
    23401 attaacaccc aacaagaatt gcacagtgta cttgctgaag caggaatcga agcaaatgac
    23461 gaacaagtta atcaggtggc taaagcaatc aagaaaattg cgggcgatgc tactcgtgat
    23521 aattttaacg cattagccaa ccctgatggt tacaaacaca tcggtcgctg caaatcggtt
    23581 gcggagttac gtactatccg cccaacggaa cacggtcagc gtattttggt ggatgcttac
    23641 tatgaggacg gcacaacagg cggtggggag tttgtggctg atttgcagga tttagttacg
    23701 cctgatgatg gtggggtttg ttttgttgtg aataataatg gggggcggtg gaagcgtgtt
    23761 gacctttcac atcttacatt atttgatttt ggggccgtcg gtgacggggt aacaaatgat
    23821 gaatcagcat ttgtaaatgc tatgagatat tctcaatttt ttatcgaaaa cggtacattt
    23881 cgtatcaaca atgccgttaa ttctgttcgc gataatgtgc aaattttggg caataagacc
    23941 ggcaaattag tattaggtgc aggtatccag caagcagagg ccgaagtttt taatattaat
    24001 cacagcaatt attttatctc gggtttttgt attgaaacac cgaacaaagc aattggtatt
    24061 cgatttaagt ctttggatga tgctggtgtt aaaaatctcc atattgacaa tgttgtgttt
    24121 aatggcacct tctacggggt cagggcaggt gaaagtattc aggcggatac taattaccca
    24181 actgataatg tgattgtaca aaactgccaa tcgtattgtg gttcaggtaa tgctggacat
    24241 tatctctgca ccaaagttaa aggtgtgcga ttttttaaca atatagcaat tggaggtcgc
    24301 aatgtatcag cttacggtgc aacatcttgc tctgatattt tcattttggg taatcgtgaa
    24361 caggggatgg cagtgacgac agttgatgtg gaagcgggcg tacaagtgga agatgtacaa
    24421 actggcgaat gtgtggtagc gaataatatt tgcagccacg atatttggat gtctggtgct
    24481 aaaaatgcca ttatcaacag caacctctgc cgtcgcttgc gtgtcacatc aggtaatccg
    24541 actgacacag gcagcaacaa tgtagaattt tcaaataatc aagcaaaatc tattgcaata
    24601 tcgaaatatg gttctttcga taatccttca ttgtttattg ataatatcaa ttttattaat
    24661 aacattttgg agcccagtga atttaatcaa gatgtgtata ttgacgggac aattttaaaa
    24721 cagtgccaat ttattaataa tcgttgtatt agcaagtcta agcaatattc tgtcagactt
    24781 gctcgcaata atccgtgctt aaatttaaca tttaagagta ataaattatc ggacaatcct
    24841 gcgattatat caggtaagca aggtgttatt ttctgcgatg atttgagctt aggagtaaat
    24901 aagcacagat taagattgcg attttcatcg ccgattaatt ttactgttaa tcaagaatgg
    24961 caaaaaatgc cgtttgacag agtgttaaat gatatcaatg gtcatgttga tattgtcagt
    25021 ggtcaattta ttgcaaggac aggcggcact tttcatttaa aaggtaaaat aacggttaca
    25081 aattcatcgg aagatgtgct gaatttatat caactcaggc tgttaataaa taacgaagag
    25141 aaagaaagac tgattaatca aagtgtttca cctaaaaaca gcaaatcaaa tacaaatggt
    25201 atgacaacaa tcaattttga tactgtagtt tatttaaaag taggtgacat tgttgatttg
    25261 cagtataaaa caacgcatga gttgacatta ctgtcagact catttttatc tcaaatttat
    25321 gtgactcagg agttttaagt gactaaaaaa cactactgca caggctggcg taatgcgcca
    25381 gccaatatca atagttgctg tcatcagcat gaccgagact acggtatcaa gggcacagtc
    25441 agccgagccg aagccgacaa gcggttaaga cattgcttaa tttcgcaagg gaatccgatt
    25501 aaggcgtggg tgttttgggt tgtggtgagg ttgtttgggt ggatttatta caaggagaaa
    25561 aatatcaatg gaaagtcata aaacaggaaa tatcattact gagcaagcaa gcaagcaagc
    25621 aagcaagcaa gcaagcaagc aagcaagcaa gcaagcaagc aagcaagcaa gcaagcaagc
    25681 aagcaagcaa gcaagcaagc aagcaagcaa gcaaagtgta gctcaattta aacaagctcc
    25741 gctacctttt acaggtcaaa agcggatgtt tttaaaacac ttttaatgat gatttaaagg
    25801 gcgtttgttt tcagtttatt ttgcataact tttgtgatta cggctatgca aaatgatttg
    25861 caaaactatg caaaataaat cgaccgctta caattctaaa agaaaagcag tctattctct
    25921 accacatttt gcaaaaaagt ggcaaaaata gaccgcttgt atggcatatc actccgataa
    25981 actcacccca taaaatggcg tgtagccaaa cggattttgc acaaaacctt gcacccgttt
    26041 gctgagtggt acataattga tagcgtgagc cagcggaata aatggcgttt gttcgttggc
    26101 aatttccacc gcttgttgat agagcttcgc ccgctcgtca cggttttggg tttgtacagc
    26161 ttgagctaat aactgctcaa atttctcatc cgtccaacgc ccataatttg ttcccggaat
    26221 attagatttg cttaataaag ggaagaggaa attatcagga tcaccattac ggcttgtcca
    26281 accataagtg ccggtagaga actcgccttc acgggtgcgt ttgttaaaat ccgcccattc
    26341 gtgggtcact aatttcgctt tcaccccgat tttcgcccaa tccgcctgaa taatttcagc
    26401 cgttcgacgt gggtttgggt tagaagggcg cactaccggt tgtacccaaa tttccgtttc
    26461 aaatccatcg gcaaagccgg cttctgttaa tagggctttg gctttttcaa ggttaaattc
    26521 atattgcggt aaatttggat tatagcccaa caccgaatca gggaacggat tacttgccac
    26581 cgtgccaccg ccttgataaa cagcctcgac aatcgctttc ttatcggttg cataatgcaa
    26641 ggcttggcga actttggcgt tatttaatgc cggtttggtg ctgtttaagc cgatataagc
    26701 taaattcagc ccgtctctct gtaacaaatt caccttcgga tcttgtttca tttgggcaat
    26761 atcggaaata ttcgggaaat caatcacatc gcattcaccc gctttcagtt tggcataacg
    26821 agtgcccgca tcaggggtaa tactaaaaat cagtttctca aattttgctt taccttggaa
    26881 ataatccggg ttcgcaacat aacgcaccgc ttgatcggtt tgataggctt ggaacacaaa
    26941 aggccctgtg ccgatcgggg cttgatccaa taactccggc ttgccttccg ccagcaattt
    27001 atccgcatat tcttttgaat aaatcgacaa gaaatccatc gctaaggttg tcagaaaagg
    27061 agcattaggt tgctgtaagg tgattttaac agtgtaatcg tccacttttt ccactgtttt
    27121 taaaatcttc ggcaaattca tccaatggta atagaaataa gtaccgtcag agaccttatg
    27181 gtaagggtgg tttaaatcca tctggcgttg gaacgagaac actacatcat ccgcattcag
    27241 cgttcgagtt ggcgtaaatt gtttattgga atgaaatttc acgtctttac gtaaatggaa
    27301 tgtgtaactt aagccatcgt cactgatttc ccaacgttcc gccaaactcg gctcaaccgt
    27361 tagttcaccg gctttaaacg ctaataagcg attataaatt tgttgtgaac tggcgttgaa
    27421 atcattggca tcattagtaa ttgcaggact aagtttcatc ggtggagttg ccacgcaatt
    27481 aatcaacgta tttaccggag ctgctgccac caaactgctg gcacttaaga gtaaaacaga
    27541 aagagataat tgagaaagac gcataaagac tccttaacat tttctactaa cttactcggc
    27601 attaacgtat tttcaaaata actaaaggtt ataatttata atcagaataa tcagttgcaa
    27661 accaatgatt cttggctaga atattggcag ttttttaaac gagagattta ctatgacatc
    27721 aatttgtatc atcaccggca gcaccttagg cggtgcggaa tatgttgccg atcacattaa
    27781 tgaggtatta agcgaacaag gttttgaagt ggaactgttt aaccaagcca cccttgccga
    27841 tattgagggc aaaaccaatc tgattatcgt tacctctacc cacggagccg gcgagttacc
    27901 cgaaaatatc caacagctat ttgacgattt agcaaatagc tcggctgatt tcagcagtat
    27961 gaaatttggt gtaatcggtt taggtagctc cgactatgac accttctgca atgcagtaaa
    28021 tattgttgaa gaagctctca aagcgaaagg ggcaagccaa gtttgcgaat cactgagaat
    28081 tgatgtaatg aataatttcg accacgatgg cagtgctgaa gaatggttac catcatttgt
    28141 tgaacaatta taacttaagt atcaatgaac aaaatcagtg caaataaggc aaagtggatt
    28201 aaaatcgcta ttatcttaat ctatatgttc agccctgtag atattctacc cgaagcgatt
    28261 ttaggcccat tcggtttggt tgatgatgcc gccgcattaa tgctattgat taagactatt
    28321 ttggaaaaat aggcatagta gacaaaatag ttgcaaaaaa ccaaaacaaa acgaccgctt
    28381 gtaacccaat acaagtggtc ttatctttta cttaattctt ttaagggaga atatctcttg
    28441 aaataggatt ttgaagtgaa ataagcgttt ctgttgactg aatttcatca atagactgaa
    28501 tttttcctgc taataccgaa tgcaactctt cgatggtatg ggtcattact ttaataaaaa
    28561 tagaataatt accagtggta tagtacgcct caacaacttc attaaattca ctaagtttag
    28621 caattacttt atcataatct ttggctgatt ttaaaataat gccaataaag caacacacgt
    28681 catacccaac cttacgctcg tccactacga ttttggtaga ttcaataatg ccggcttggc
    28741 gcattttttc aacacggaca tggatagttg cggcactcac accaaaaact tttgccattt
    28801 ccgcataagg cgtgcgagca tctttaatta gccaacgtaa aattttttga tccagcttat
    28861 caatttccga tttagtcgtg tttt
//
LOCUS       DANLYI010000009        27093 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.009, whole genome shotgun sequence.
ACCESSION   DANLYI010000009 DANLYI010000000
VERSION     DANLYI010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 27093)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27093)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27093
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.009"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            4..957
                     /gene="trxB"
                     /locus_tag="PXD81_000299"
     CDS             4..957
                     /gene="trxB"
                     /locus_tag="PXD81_000299"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005595726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HDL5536368.1"
                     /translation="MTTKHTKLLILGSGPAGYTAAVYAARANLNPVLVTGMQQGGQLT
                     TTDEIENWPGEFEQITGTELMNKMLKHAEKFETEIVFDHIHSVDLSKRPFTLNGDLYT
                     FTCDALIIATGASAKYLGLPSEEHFKGKGVSACATCDGFFYRNKPVAVVGGGNTAVEE
                     ALYLANLASEVHLIHRRDSFRAEKILLGRLQKRIEEGKIILHTDRTVDEVLGDNMGVT
                     GLRLKSTKEELSEEIKVDGFFVAIGHAPNTAMFEGQLELENGYIKVKSGLEGNATATS
                     VEGVFAAGDVMDHNYRQAITSAGTGCMAALDAERFLDAQEA"
     gene            967..1722
                     /gene="kdsB"
                     /locus_tag="PXD81_000300"
     CDS             967..1722
                     /gene="kdsB"
                     /locus_tag="PXD81_000300"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HDL5536369.1"
                     /translation="MKFTVIIPARYASSRLPRKPLLDIAGKPMIQHVWEKAQLAGASR
                     VIIATDHPEIEALAQSFGAEVCMTSEKHNSGTERLAEVIEKMQIADSEIIVNIQGDEP
                     LIPPVIVTQVAQNLDAYQVNMATLGVKLATREELFNPNAVKVVADKNGMALYFSRASI
                     PFARDSFPDCDDSFVAAQGYLRHIGIYAYRAGFVKQYVAWQPTQLEQLESLEQLRALW
                     HGEKIHIEPAKEAPQVGVDTPEDLERVRAILAR"
     gene            1800..2219
                     /locus_tag="PXD81_000301"
     CDS             1800..2219
                     /locus_tag="PXD81_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF937 domain-containing protein"
                     /protein_id="HDL5536370.1"
                     /translation="MLGNILGSLASSVLSGNNQSTATQLIQNLLASQGGIEGLIAKFQ
                     QGGLDDVLRTWISSDEDNAPVSGEQISNVFGQENIQNVAQEAGVDAADASDLLAQFLP
                     KIVDTLTPNGNVEDLKNFDANDLLAQAAKGILGNLFK"
     gene            complement(2273..3259)
                     /locus_tag="PXD81_000302"
     CDS             complement(2273..3259)
                     /locus_tag="PXD81_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HDL5536371.1"
                     /translation="MSQLIRTLKGHIRDEIIKKGGWVNAHAHADRAFTMTPEKITVYQ
                     NANLQQKWDLVDEVKRNSSVEDYYRRFSQAIELMISQGVTAFGTFVDIDQVCEDRAII
                     AAHKAREVYKNDIILKFANQTLKGVIEPNAKKWFDIGSEMVDMIGGLPYRDELDYGKG
                     LEAMDILMDKAKSLGKMLHVHVDQFNSPKEKETEQLCDKSIEHGMQGRVVAIHGISIG
                     SHPKEYRKMLYQKMRDSQMMVIACPMAWIDSGRKEDLQPFHNALTPADELIPEGITVA
                     IGTDNICDYMVPLCEGDMWQELSLLSAGCRFTNLEEMANIASINGRKVLGLV"
     regulatory      3543..3686
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            3730..4683
                     /gene="thiB"
                     /locus_tag="PXD81_000303"
     CDS             3730..4683
                     /gene="thiB"
                     /locus_tag="PXD81_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005600219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HDL5536372.1"
                     /translation="MKFILSSLALLSASVYANQPTLTVYTYSSFASEYGPAPKLEQNF
                     EKECGCDVKFVPLDDSVTMFNRLRLEGAKTKADVVVGIDNFLQPEAEKSGLFAEFEAK
                     TKLETQQKIFFPYDTGSYAFIYDKEKLKNPPKSLKELVERQDLKVIYQDPRTSSVGRG
                     FLMWVNQVYGDEAPTAWKTLAKHTVTVGKGWSETYGAFLKGESDLVLSYTTSPLYHKW
                     HENTDKYVAAPFEEGHLVQVENAAIVKSSQQKALANQFLEFLHKPESQHIISYHNVMN
                     PVLTDGVDPAFAAMPTYKTIEFTPPTTETIQKWLADWSKSW"
     gene            4933..5379
                     /gene="dksA"
                     /locus_tag="PXD81_000304"
     CDS             4933..5379
                     /gene="dksA"
                     /locus_tag="PXD81_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005595916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HDL5536373.1"
                     /translation="MTQAAKTSLGLLALAGVEPYELKKDEEYMNEAQREHFTKILKAW
                     HSQIMEEAERTKSQLQEDMTNFADPNDRASQEEEFSLELRNRDRERKLLKKIEQTLYK
                     IQEDEYGYCETCGVEIGIKRLEARPTADLCIDCKTLAEIREKQLGL"
     gene            5624..6955
                     /gene="pcnB"
                     /locus_tag="PXD81_000305"
     CDS             5624..6955
                     /gene="pcnB"
                     /locus_tag="PXD81_000305"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HDL5536374.1"
                     /translation="MLHKKFAVNASQYRISPKDFPKNAISIVDKLQIAGYEAYIVGGC
                     IRDLLLEKKPKDFDIATNARPEEIQRIFGRQCRLIGRRFRLAHIVYGREIFEVATFRA
                     GHESHRNDKISKVSDEGMLLRDNVYGTLREDAERRDFTVNALYYDIKRNLIFDFFNGI
                     EDLRAGKLSLIGEPTIRYQEDPVRMLRAVRFMAKLDMFLDKATASPIRDMAVLLTNIP
                     AARLFDESLKLLQTGAGLKTYQLLREYELWQFLFPVLPAFFTAKGDSYAERMITKALN
                     STDERIADKLPVNPAFLFAALLWYPLREKMEQLKNEGGFNSYDAMMLAANDVLAESCN
                     AIALHRRHTSVIRDIWALQFQMMKRSGNRPQQTLEHVKFRAGFDLLVMRAEIEGGDLV
                     ELSAWWHEYQFSNEGQRTELLKSVRHLGNVPSEKKKTRRRRTFKKKPKVNG"
     gene            7158..7631
                     /gene="folK"
                     /locus_tag="PXD81_000306"
     CDS             7158..7631
                     /gene="folK"
                     /locus_tag="PXD81_000306"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HDL5536375.1"
                     /translation="MQTVYIALGSNLENPLEQLKQAVEKLKKFANEFEISPFYGSKPV
                     GPQDQPDYVNAVAKFSTDLSPTALLDKLQSIENEQGRVRVRRWGERTLDLDIILYGNQ
                     QIQTERLNVPHIEMKNREFVIVPMFDLTPDLVLPTGEKLADIYAQFKDHQMIIFS"
     gene            complement(7628..7822)
                     /locus_tag="PXD81_000307"
     CDS             complement(7628..7822)
                     /locus_tag="PXD81_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HDL5536376.1"
                     /translation="MKPATFYFAIFLLNLTACTPKVQLETPSEGITINMNVVVDHRID
                     VKMDEKSRAVITKSENKTKN"
     gene            complement(7840..9615)
                     /locus_tag="PXD81_000308"
     CDS             complement(7840..9615)
                     /locus_tag="PXD81_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH domain-containing protein"
                     /protein_id="HDL5536377.1"
                     /translation="MPLKTAIRISLLSQNSWGKGNIVISNSDGEITEKGLNLPLRLTG
                     NIKHGNFILYSSVPLDFQGNFDDLSVKFLPSSLLRLTGKERYLTIEDLRFPLAGIKID
                     KHGIHGRLHAILRGQSPDFNNIEFHLDGQAQNFKAGALDFFQDPKDANAVKDSWKWRI
                     WGASILKAFNSKVNLSGRGQWHKQLVRLEELKGDLATVKLADTTISKITLNNTQAIRF
                     NVKKFTLDGAVMLQSPEIAFSYGGVLPKPRVNLAFDGEVEKLRFKGAVNTTELGPLKL
                     FARRQLSQDASQIIGKLYWPEQSAQGFQPLFPFRSQWVINNGTIRGETAFSAGSKNGL
                     IAGGHLAIRNGGLSLPNGEAKGIEFSLPYRYQNGRFHFGAKKPLDVKIAQIKLGDLAL
                     DNASMKLNGYYPYTKAKPLNLNQLSFDLFGGKLNVKRFALPQTELAYLNLERIDFEQI
                     LQFMQYQQIDLKGKANAILPFWLSGKPCYICDGLLTQAETSYLTFTPELLSEMQKSGY
                     TEQILLHLVDKSTINDFRSLINVGPKGDLVLDGKLKLSSNQNKSKVNLNYNHRENLFD
                     LWHLINYGSQFEQKLENYLYQKLER"
     gene            complement(9801..10547)
                     /locus_tag="PXD81_000309"
     CDS             complement(9801..10547)
                     /locus_tag="PXD81_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5536378.1"
                     /translation="MKRALKFFGIATVLSVVAIYYFLMPSHYLATPINWILPKAWKVQ
                     LPQGLNLNWQGSSLAQFQLSYRDCPLLSADKTDLGWIEKNHLSVDKLDIDYRCLALFP
                     QNEHSNSDTSLNALLAVIPDGEATIHALNWKNLPTDFNPRLQRLFEQTAIKLSLSKGE
                     IQATAAQQAVKFAANFANGRLNADLSYQPNEKEQHNLRISAEIEDNFTQLPPTISAEY
                     DWQLSYEIIANKALQKGALFSVGKKMPHKS"
     gene            complement(10551..11366)
                     /locus_tag="PXD81_000310"
     CDS             complement(10551..11366)
                     /locus_tag="PXD81_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5536379.1"
                     /translation="MAHDHDSPSKLEISTHAALPMVLMLFIEMALNFYSAPKNSIFIS
                     PYLLAFFVTSLISFLVLWKGEICRGQASRLSFILPFLLIFAVGNFLYSIGFTPKHNPM
                     AVASIAAVFACLIYFRLPEDQTLYNTLMYCGLAISSIGIIQYLAIYWFELPSLFNGIR
                     ANNFAQVLLGILLAGWYLMLAKSRLDVFLKLLVKTALVVLILNYCWTAFVLYQHLQIM
                     PDMPLLSFIIYFAAQFVILLMLAWLLLGKNIKNPTAWTVATLLAILYPFANVV"
     gene            11456..11863
                     /gene="gloA"
                     /locus_tag="PXD81_000311"
     CDS             11456..11863
                     /gene="gloA"
                     /locus_tag="PXD81_000311"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009875026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HDL5536380.1"
                     /translation="MRILHTMLRVGDLERSIKFYTDVLGMRLLRRSENEQYKYSLAFL
                     GYADESESAVIELTYNWGVDKYELGTAYGHIALGVDDIYKTIEDVRAAGGKITREPGP
                     VLGGTTVIAFAEDPDGYKIEFIENKDAKAALGN"
     gene            11948..12595
                     /gene="rnt"
                     /locus_tag="PXD81_000312"
     CDS             11948..12595
                     /gene="rnt"
                     /locus_tag="PXD81_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010130043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HDL5536381.1"
                     /translation="MEQTTEQIDYKLLKHRFRGYLPVVIDVETAGLNAQTDALLELAA
                     ITLKMDEQGYLVPDQQCHFHIAPFEGANINPESLKFNGIDIDNPLRGAVAENIAIPEM
                     FKMVRKAMKEQGCQRAVIVAHNAAFDQGFLQAAIKRINAKRDPFHPFAMFDTATLAGF
                     MYGQTVLVKACQVAKIPFDGKQAHSALYDTERTAELFCAMVNRLKDLGGFPPLSD"
     gene            13016..14773
                     /locus_tag="PXD81_000313"
     CDS             13016..14773
                     /locus_tag="PXD81_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin-binding protein-like solute binding
                     protein"
                     /protein_id="HDL5536382.1"
                     /translation="MFKLKSSFVLLNAALLAACSSNGGSFDVQSAKVESQTQTTPKKP
                     SLQDDNSNARRTVSASETEALLQPGFGFSAKIPRRNLFPQGKEDVAPIGDIKEITGDL
                     TKIPYEEEVKAYGSSADGFSHTHDRNHKLYTRDFNFVRSGYVLHSGAKPEIKPKEILR
                     TGAHGYVYYLGIEPAKAIPTQKATYKGYWDFTTDARKGRDSKYFSNSAGINIGATPEN
                     SYDINVDDSEKPMGHTGEFTADFANKTLTGTLVRNGYVSRSKEQKITTIYDIDAKIKG
                     NRFSGKANAKNTDDPYFGKSSTTLEGGFFGGKAQELAGKFLADDKSVFVVFAGKRDAK
                     KDDSESAFDAFKIKLKDLNKSEMDTFGNATHLIINNKQIPLIAEGKKSFAEMKFDDLV
                     TRTIDGKTYRVSVCCNNLDYVKFGTYSEGNNSDTDTAHQYLVGERTAVADLPTGTVKY
                     RGTWDGVMYSKSGSAGAESPSNSESGTRSLFDVDFVNKKINGKLIANDGVEERPMLTL
                     EGNLKGNGFAGTAKTGNSGFNLDPKSTNGGTVVHINTQFEGGFYGPKATELGGIVHSA
                     ETDKDRVSITFGGKRQIEK"
     gene            14867..17653
                     /locus_tag="PXD81_000314"
     CDS             14867..17653
                     /locus_tag="PXD81_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoferrin/transferrin family TonB-dependent
                     receptor"
                     /protein_id="HDL5536383.1"
                     /translation="MKYHHFRYSPVALTVLFALSHSYGAATENKKIEENNDLAVLDEV
                     IVTESHYAHERQNEVTGLGKVVKNYHEMSKNQILGIRDLTRYDPGISVVEQGRGASSG
                     YAIRGVDKNRVSLLVDGLPQAQHYHTLGSDANGGAINEIEYENIRSIELSKGASSAEY
                     GSGALGGAIGFRTKDAQDIIKEGQHWGLDSKTSYASKNSHFLQSIAAAGEAGGFEALV
                     IATHRHGKETKIHSEANKLKHNIRRITGFENRYDLYPNSAQNAPGGSFFIVEDTCPTL
                     DCTPRARVKLNRDNFPVRTFPEYTPEERKQLEQIPYRTEQLSAQEYTGKDRIAPNPLD
                     YKSNSVFMKFGYHFNSSHYLGAILEDTKQRYDIRDMQTPAYYTKDDINLSLRNYVYEG
                     DNILDGLVFKPRIPYGLRYSHVKFFDERHHKRRLGFTYKYKPENNRWLDSIKLSADKQ
                     DIELYSRLHRLHCSDYPVVDKNCRPTLDKSWSMYRTERNNYQEKHRVIHLEFDKALNA
                     GQGVFKQTHKLNLGLGFDRFNSLMDHGDMTAQYTKGGYTSYRGRGRLDNPYIYRRDPR
                     SIETVSLCNNTRGDILNCEPRKIKGDSHFVSFRDLVISEYVDLGLGVRFDQHRFKSDD
                     PWTLSRTYRNWSWNGGITLKPTEFVSLSYRISNGFRVPAFYELYGKRNHIGLKDNEYV
                     QRAQRSHQLEPEKSTNHEIGVSFKGQFGYLDVSYFRNNYKNMIATACKRIIQKSHCFY
                     NYHNIQDVALNGINLVAKFDLHGILSMLPDGFYSSVAYNRVKVKERKLTDSRLDSVND
                     PILDAIQPARYVLGFGYDHPEEKWGIGITTTYSKAKNADEVAGTRHHGIHRVDLGGKL
                     TGSWYTHDITGYINYKNYTLRGGIYNVTNRKYSTWESVRQSGVNAVNQDRGSNYTRFA
                     APGRNFSLAFEMKF"
     gene            complement(17758..18054)
                     /gene="fis"
                     /locus_tag="PXD81_000315"
     CDS             complement(17758..18054)
                     /gene="fis"
                     /locus_tag="PXD81_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011199710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HDL5536384.1"
                     /translation="MLEQQPTQNPLTVTMLNAQAQQVNKPLRDNVKAALKNYLSQLNG
                     EDPTELYELVLSEIEHPMLDMVMQYTRGNQTRAATMLGINRGTLRKKLKKYGMG"
     gene            complement(18059..19048)
                     /gene="dusB"
                     /locus_tag="PXD81_000316"
     CDS             complement(18059..19048)
                     /gene="dusB"
                     /locus_tag="PXD81_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HDL5536385.1"
                     /translation="MQIGQYAIKNRIFLAPMAGITDQPFRRLCSQLGAGLTFSEMMST
                     NPDVWHTEKSRLRLAHHDEIGLNAVQIAGSDPVEMAKAAKINVEYGADIIDINMGCPA
                     KKVNKNMAGSALLRNPKLVAQILDSVVNAVDVPVTLKIRTGWDQENRNCLQIAKIAEQ
                     AGIKALTIHGRTRSCLFNGEAEYESIKAVKEAISIPVIANGDIDSAEKAKFVLDYTKA
                     DAIMIGRASFGNPWIFKEINDFFETGLSSPLSIDKKYQLMFKHIEDLHQFYGEEKGYR
                     IARKHVGWYTEKLNPESNFKRTFNTLSSTKEQLYALEDFVKSIHLESQLRKVG"
     gene            complement(19272..19760)
                     /gene="sixA"
                     /locus_tag="PXD81_000317"
     CDS             complement(19272..19760)
                     /gene="sixA"
                     /locus_tag="PXD81_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HDL5536386.1"
                     /translation="MNIWIMRHGEAGFNASSDAARCLTDNGIKNTISQGKWLGEHLAN
                     QQIQLDKILVSPYLRAQQTLENVLIGMQAVNFPQHFTNLIESWDEVTPDGNPHTVESY
                     LHFLREEGAKNILLISHLPLVFDLVQILTNNQSNVQFPTSTIAEINWVKTKAELIRVK
                     HT"
     gene            19887..21326
                     /gene="pyk"
                     /locus_tag="PXD81_000318"
     CDS             19887..21326
                     /gene="pyk"
                     /locus_tag="PXD81_000318"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005818583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HDL5536387.1"
                     /translation="MSRKLRRTKIVCTMGPATDRGNTLEKIIAAGANMVRMNFSHGIP
                     EDHIERANKVREIAARLGRHVAILGDLQGPKIRVSTFKDGKIFLNIGDKFTLDADLPR
                     GEGHQEAVGLDYKNLPNDVVPGDILLLDDGNVQLKVLEVEGVKVHTEVTVGGPLSNNK
                     GINKLGGGLSAPALTDKDKEDIKLAAKIGVDYLAVSFPQSSADLHYARELAKEAGLEA
                     KIVAKVERAETVATEEAMDDIILGADVVMVARGDLGVEIGDAALVGVQKRLIRRARKL
                     NRVVITATQMMESMIKKPMPTRAEVMDVANAVLDGTDAVMLSGETANGDYPVETVKSM
                     AEVCLGAETMPSINISRHRMEGTFKSIEEAVAMSAMYTANHLEGVSAIISLTHSGETA
                     KLTSRISSGLPIYAMSRNQKALNLCALYRGVTPVYYNEDSRTMEGAKKAIAQLKEQGY
                     LLTGELVLLTHGDELKAGGTNTCRILRVE"
     gene            21422..22459
                     /locus_tag="PXD81_000319"
     CDS             21422..22459
                     /locus_tag="PXD81_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005600238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HDL5536388.1"
                     /translation="MHNKLLSICILRLSAIGDVCHTLAVIQAIQRKYPSAEITWIIGK
                     TEAYLMKDIPNVHLIPYDKKIGWKGIFTLWQKLSHKRFDFLLNLQTAFRASILSLGIK
                     AKKKIGFNQDRAREMQWLFTNQKVEMTDSPHVLDGQMMFAKAIGIMDLSAQWNLPVSD
                     EDLAYGKQFIDFTRKNILIAPCSSKKEKDWLPERYAEIANWLSKQNINVLIAGSPSQY
                     EMETAAKIQQLAPNCTSIAGKTTLKQLAGVIRQVDLVISSDSGPAHIATTQNTPVIGL
                     YAIHNPRRTGPYNDLDKVVSVYDEAILQSYGKPWQQLAWATKAKGKNWMEKITVESVK
                     QKVVETLKITL"
     gene            22499..23557
                     /locus_tag="PXD81_000320"
     CDS             22499..23557
                     /locus_tag="PXD81_000320"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005600235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HDL5536389.1"
                     /translation="MNTVFNQDSVHNVNIQDEKVLLTPQELKQEFPLPEHLRNQIERS
                     RKTISDIIHRRDKRQLIVIGPCSIHDPVAALEYGKKLKALSEQVSDKLYIVMRVYFEK
                     PRTTVGWKGLINDPKMDGSFDVETGLRIARKLCLDLAELGLPLATEALDPMTPQYLAD
                     LFSWSAIGARTTESQTHRELASGLSMAVGFKNGTDGDLAVALNAMQSSAQSHSFIGIN
                     QQGQVTLLKTKGNADGHIILRGGKTPNYEKQYVDDCEWVLKQANLPTAIMIDCSHGNS
                     NKDYRRQPLVAENVLEQLLAGNTSIIGLMIESNLHAGNQSSELPFEQMKYGVSITDAC
                     IDWQTTEELLTHFAEKLR"
     gene            23612..24733
                     /gene="tyrA"
                     /locus_tag="PXD81_000321"
     CDS             23612..24733
                     /gene="tyrA"
                     /locus_tag="PXD81_000321"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009875025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HDL5536390.1"
                     /translation="MNPLAPLREQIDQVDQQLIALLSERLALVAEVGKVKSEHGIPVY
                     APERETAMISARRSEAEKQGIPADLIEDVLRRIMRESYANENKHGFKRVNSDIQKIVI
                     VGGKGKLGGLFGNYLTLSGYNVEALGRDDWDNAEQILANADVVIVCVPIANTLATIER
                     LQSYLTENMILADLTSVKHQPLQKMLEVHNGAVVGLHPMFGPDIASQAKQVVACCHGR
                     FPERYQWLLEQIKIWGAKIEEIEAAAHDNAMTYIQALRHFSTFAFGLHLSRQPVQLSQ
                     LLALSSPIYRLELAMIGRLFAQDGSLYADIIADKPENLATIETLKESFEQGLDFFKRN
                     DKAGFIKAFEEVHHWFGDYSEQFLKESRVLLQQAHDSRK"
     gene            complement(24924..26009)
                     /gene="recF"
                     /locus_tag="PXD81_000322"
     CDS             complement(24924..26009)
                     /gene="recF"
                     /locus_tag="PXD81_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HDL5536391.1"
                     /translation="MALTRLLINHFRNIQHTDLAFSPHFNFLVGANGSGKTSLLESIF
                     YLGHGRSFKSHISNRIIHYDKDDFVLHGKIDEAKHSWSVGIQKFRSGETTLKINGEDG
                     NKIADLAHLLPMQVITPEGLTLLNGGPSFRRAFLDWGLFHQHPDFYAHWNNLRRLLKQ
                     RNSALQQVRSYQELKAWDIELVKTTYAVSEMRAEYAEALRPEIEKTCQFFLPEIEIGL
                     SFHQGWEKGADYAEILAQGFERDKALGYTMIGAQKADFRFKANGLPVEDVLSRGQLKL
                     LMCALRLAQGEHLVAQKQRQCLFLIDDFASELDPTKRELLAHRLRESGSQVFVTAITQ
                     DQLNQMQWQDRSDDCLFTVKNGEIQPQ"
     gene            26149..26478
                     /locus_tag="PXD81_000323"
     CDS             26149..26478
                     /locus_tag="PXD81_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HDL5536392.1"
                     /translation="MFGKGGLGGLMKQAQQMQERMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRIEIDPSLMEDDKEMVEDLVAAAFNDAVRRAEEMQKEKMASVTAGMQLP
                     PGMKFPF"
     gene            26486..27091
                     /gene="recR"
                     /locus_tag="PXD81_000324"
     CDS             26486..27091
                     /gene="recR"
                     /locus_tag="PXD81_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015939588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HDL5536393.1"
                     /translation="MQTSPLLENLMEALRALPGVGPKSAQRMAYHLLQRNRSGGVALS
                     KALNEAMSHIGHCKSCRTFTEEEECTICKNPRRQMSGQLCVVEMPEDIQAIEQTGQFS
                     GRYFVLMGHLSPIDGIGPREIGLDLLQQRLEQESFHEVILATNPTIEGDATANYIAEM
                     CRMYNVKATRIAHGVPVGGSLEMVDGTTLSHSFAGRRDIDI"
BASE COUNT         8150 a         5106 c         5932 g         7905 t
ORIGIN      
        1 attatgacca ctaaacacac caaactttta attttaggct cagggcctgc cggttatacc
       61 gctgctgttt atgccgcccg tgccaatcta aacccggttt tagtcaccgg tatgcaacaa
      121 ggcggacagc tcactacaac cgatgaaatc gagaactggc cgggggaatt tgaacaaatt
      181 accggcacag agttaatgaa caaaatgctc aagcacgctg aaaaatttga aactgaaatc
      241 gtgtttgatc atattcattc agtggattta tctaaacgcc cattcacgct aaacggagat
      301 ttatacactt tcacctgtga tgcgttaatt attgccaccg gtgcgtcggc gaaatattta
      361 ggtttgcctt ctgaagagca tttcaaaggc aaaggcgtgt cggcttgtgc aacttgtgat
      421 ggtttcttct atcgcaataa accggttgcg gtagtcggtg gtgggaatac tgctgtggaa
      481 gaagcacttt atttggcaaa tttggcaagt gaagtgcatt taattcaccg ccgtgatagc
      541 ttccgtgctg aaaaaatctt gcttggtcgt ctgcaaaaac gtattgaaga aggcaaaatt
      601 attctgcaca cagaccgtac ggttgatgag gtgttaggcg ataatatggg cgttaccggc
      661 ttacgtttaa aatcaaccaa agaagaattg agcgaagaaa ttaaggttga tggtttcttt
      721 gtggcgattg ggcacgcacc gaatacggca atgtttgaag gtcaattaga acttgaaaac
      781 ggctacatta aagtgaaatc aggtttggaa ggcaatgcta ctgccacttc tgtggaaggc
      841 gtatttgctg ccggtgatgt gatggatcat aactatcgcc aagcgattac ctctgccggc
      901 acaggttgta tggcagccct ggatgcagaa cgtttcttag atgctcaaga agcataagga
      961 tagcctatga aattcactgt aattattcct gctcgttacg cttccagccg tttgcctcgt
     1021 aagccgttgt tggatattgc aggcaaaccg atgattcaac acgtttggga aaaagctcag
     1081 cttgccggtg caagccgtgt gattattgcc accgatcacc ctgaaattga agccctcgct
     1141 caatcttttg gggcggaagt ctgtatgact tcggaaaaac acaattccgg cactgagcgt
     1201 ttggcggaag tgatcgaaaa aatgcagatt gccgacagcg aaattattgt gaatattcag
     1261 ggtgatgaac cgctgattcc accggtaatt gttactcagg tagctcaaaa tttagatgct
     1321 tatcaagtca atatggcaac actcggtgtg aaattagcga cccgagaaga gctatttaac
     1381 ccaaatgcgg tgaaagtggt ggcagacaaa aacggaatgg cactttattt ttcccgagcc
     1441 agcattccgt ttgccagaga cagttttccg gattgtgatg acagttttgt tgccgctcaa
     1501 ggttatttac gccatatcgg catttacgct taccgagccg gttttgtgaa acaatatgtg
     1561 gcgtggcagc cgacacaatt agaacagctt gagtcccttg agcaactaag agccttatgg
     1621 cacggtgaaa aaattcatat cgaaccggcg aaagaagccc cgcaagtggg ggtggatacg
     1681 ccggaagatt tggaaagagt gcgagccatt ttagctcgtt aaagtgatta caagcggtcg
     1741 aagtttgctg tttttttgca aaattttcgc aaaattgtac cgcttgcata ggagaaatta
     1801 tgttaggaaa tattttaggt tcattggctt cttcagtatt gagtggcaat aaccaatcaa
     1861 ctgcaactca attaattcaa aatttactcg cctcacaagg tgggattgaa gggctgattg
     1921 ccaaattcca acaaggcgga ttagatgatg tgttacgcac ttggatcagc agcgatgaag
     1981 acaatgcacc tgtttcgggc gaacaaatca gtaatgtgtt cgggcaagaa aacattcaaa
     2041 atgtggctca agaagcgggt gttgatgctg ctgatgcgag cgatttatta gcccagttct
     2101 tgccaaaaat cgtggatact ttaaccccga atggtaatgt tgaagactta aaaaactttg
     2161 atgcaaatga cttattagct caagcggcaa aagggatttt gggtaacttg tttaaataat
     2221 cggttatgct gttattaaag gcgaacctag ggttcgcctt tcgtttattc agttagacta
     2281 aacccagtac ttttcgacca ttgatactgg caatatttgc catttcttct aggttagtaa
     2341 aacgacagcc tgctgataaa aggctgagtt cttgccacat atcgccctcg caaagcggta
     2401 ccatataatc acagatatta tctgtgccga tagcaacagt aatgccttca ggaatgagtt
     2461 catcagcagg ggttaaagcg ttatggaacg gttgtaaatc ttccttacgt ccgctatcaa
     2521 tccacgccat cggacaggca atgaccatca tttggctatc acgcattttt tggtaaagca
     2581 tttttcgata ttctttcggg tgcgagccga tggaaatccc gtgaatggca accactcgcc
     2641 cttgcatacc atgctcaatc gatttatcac acagttgttc ggtctctttt tctttaggtg
     2701 agttaaattg atcaacgtgg acgtggagca ttttgccgag cgatttggct ttatccatta
     2761 aaatatccat tgcttccaag cctttgccgt aatccagttc gtcacggtaa ggcaagccac
     2821 caatcatatc caccatttcc gagccgatat cgaaccactt tttcgcattg ggttcaatca
     2881 cgcctttcaa cgtttggtta gcaaatttca agatgatgtc gtttttatat acttcacggg
     2941 ctttgtgagc ggcaataatg gctctgtctt cacatacttg gtcgatatcc acaaaagtac
     3001 caaatgccgt taccccttgt gaaatcatca attcaatcgc ttgggaaaag cggcggtagt
     3061 aatcttctac gcttgaatta cgttttacct catcgaccaa atcccatttt tgctgtagat
     3121 tcgcattttg gtaaacggtg attttttcag gcgtcatcgt aaatgcacga tctgcgtggg
     3181 cgtgtgcatt tacccaaccg ccttttttga tgatttcatc acgaatatgt ccttttaggg
     3241 tacgaatcaa ttggctcatt tttattcctt ttctctctgt taatgtggct caaccgcttg
     3301 cggtaattgc aagcgtgcga aaaaaaatcg ctataacagc gattttgaat ggtttgaaat
     3361 gaatagaaaa acagctacgc ctctaaagtt gaagcgtagc tgttgggaat ttgatttaac
     3421 cgatgttgtg caattcattt ctagttcctt gtttggacta gggcggtagt atacggattt
     3481 ttaatacaaa tagcaaacgt ttgctttggt tgaaatctgc ctgcaaactt tttacaatga
     3541 agccgttctc attggggtgc aacaagcggt tgtttttgcg taaaaatttg caaatttttg
     3601 ccgaaattta accgcttgtg gctgagaaat acccatagaa cctgatctga ttaatatcag
     3661 cgtagggatt tgagatagca acttgccttt cttaaatcct tctattcatt taatttagaa
     3721 ggaattgtta tgaaatttat cctctcttca cttgcgttat taagtgcaag tgtttatgcc
     3781 aatcaaccga ccttaaccgt ttacacttac agctcgtttg cttcggaata tggacctgct
     3841 cctaaattag agcagaattt tgagaaagag tgtggctgtg atgtcaaatt tgttccgctt
     3901 gacgatagcg taacgatgtt caaccgcttg cgtttggagg gagcaaaaac caaagctgat
     3961 gtggtggtcg gtattgataa tttcctgcaa cctgaagccg aaaaaagtgg cttgtttgcc
     4021 gagtttgaag caaagaccaa attagaaacc caacagaaaa ttttcttccc ttatgacacg
     4081 ggttcttatg cttttattta cgataaagag aagctgaaaa atccaccaaa aagtttaaaa
     4141 gagttggtgg aacgccaaga tttaaaagtg atttaccaag acccacgtac cagcagcgtt
     4201 gggcggggct tcttaatgtg ggtaaatcaa gtgtatggcg atgaagctcc aactgcttgg
     4261 aaaacattgg caaaacatac ggtaacggta ggcaaaggtt ggtctgaaac ttatggagcg
     4321 ttcttaaaag gcgagtcgga tttggtacta agctatacta catcgccttt atatcataag
     4381 tggcacgaaa acaccgataa atatgtggct gctccgtttg aggaagggca tttggtgcaa
     4441 gtagaaaatg cggctattgt caaaagcagc caacaaaaag cattggcaaa tcaatttctt
     4501 gaatttttgc acaaaccgga atcgcaacat attatttctt accacaacgt aatgaatccg
     4561 gttttaaccg acggcgttga tccggctttt gcggcaatgc caacctataa aaccattgaa
     4621 tttacgccac cgactactga aactatccaa aaatggttag ccgattggtc aaagagctgg
     4681 taaaaagtgt ttgaaaaata gccgaatgga tgttcggcta tttttttgtg caatcaaatc
     4741 aatttgaatg caaaattttt gcaaattcat ttgattaatg agtcgttttt gtgttattta
     4801 tagccgccat tatttaaaac ccgaagatgt tgattttttc gtatagtgcg gttacaagga
     4861 agttagtctg tattcgggca ataattttaa caataaaact tccaataaac agggaacaag
     4921 gagtaaatat ctatgactca agcggctaaa acctcactcg gtttattagc attagcaggc
     4981 gttgagcctt atgagcttaa aaaagacgaa gaatatatga atgaagctca acgtgagcat
     5041 ttcaccaaaa ttctgaaagc gtggcacagt caaattatgg aagaagcgga gcgtaccaaa
     5101 agccagcttc aagaagatat gactaacttt gccgatccga acgaccgagc cagtcaagaa
     5161 gaagagttca gtttagagct tcgtaaccgt gatcgtgaac gcaaattatt gaaaaaaatt
     5221 gagcaaacct tgtataaaat ccaagaagat gaatatggtt attgcgaaac ctgtggcgta
     5281 gaaattggga ttaaacgttt agaagcacgc ccaacagctg atttatgtat tgactgtaaa
     5341 actttagctg aaattcgaga aaagcagcta ggcttataat caactttaca gggtgcctct
     5401 tttttaagag gcatttacta tttagaggtg tattattttt aattatatta aatcattatt
     5461 ttccaagcct aaaaagtcgg ctcagaaaaa tgaaaaggta gagagtgaaa aaagagcgga
     5521 aagagaaccc aaaacttccc gttctaaacg cccagccccg aacttaacgg accaaaagac
     5581 ttatagaggt gggaagaaac aacaagataa tcctccctcc catttgttac acaagaaatt
     5641 tgcggtgaat gccagccaat atagaattag cccgaaggat tttcctaaaa atgcgatttc
     5701 gattgtcgat aaattgcaga ttgccggtta tgaagcttat attgtgggtg gctgtattcg
     5761 agatctactc ttggagaaaa agcctaaaga tttcgatatt gcgactaatg cgagacctga
     5821 agaaattcag cgtatttttg gtaggcaatg ccgattaatc ggacgtcgtt tccgtttggc
     5881 acacattgtt tacgggcgtg aaatttttga agtggcgact ttccgtgcag gacacgaatc
     5941 tcatcgcaat gacaaaattt ctaaagtcag cgatgaagga atgctactgc gtgataatgt
     6001 ttacggcacg ctccgtgaag atgcagagcg ccgtgatttc accgtaaacg ccttatatta
     6061 cgatataaaa cgcaatctta tttttgattt cttcaacggt attgaagact tacgagcagg
     6121 taaactttcg cttatcggag agcctactat tcgttaccaa gaagatccgg tgagaatgtt
     6181 gcgagccgtt cgttttatgg cgaagttgga tatgttcttg gataaagcca cggcctcccc
     6241 aattcgggat atggcggtgt tgttaaccaa cattccggca gcccgtttgt ttgacgaatc
     6301 actcaaatta ttacaaaccg gtgcagggct gaaaacttat cagcttttgc gtgaatatga
     6361 actatggcaa ttcttgtttc cggtattgcc ggcatttttt actgccaaag gtgattccta
     6421 tgccgaaaga atgattacca aagcgttaaa ttcaactgat gagcgaattg ccgataaatt
     6481 accggtaaat cctgcgtttt tatttgctgc actactttgg tatccgctcc gggagaaaat
     6541 ggagcagctt aaaaacgaag gtggcttcaa cagctacgat gcaatgatgc tggcagcaaa
     6601 tgatgtgctg gcggaaagtt gcaacgcgat tgcgttgcac cgcagacaca cttcagtcat
     6661 tcgagatatt tgggcattgc aatttcagat gatgaaacga agtggcaacc gaccgcaaca
     6721 aacccttgaa cacgtcaaat tccgtgcagg gtttgattta ttagtgatgc gtgcagaaat
     6781 tgaaggcggc gatttggtcg aactttccgc ttggtggcac gaataccaat ttagcaatga
     6841 aggtcagcgt actgaattgc tgaaatcggt tcgtcattta ggcaatgtgc ctagcgagaa
     6901 aaagaaaaca cgccgccgta gaacctttaa gaaaaaaccg aaagtaaatg gataattaat
     6961 atatttccct ctccccttgt gggagaggga gcgatttttc ttcgttcaga agaaaaatca
     7021 gggagagggg aaatgtgtaa aaaatttaat aaaagttacc gcttgtcccc tctctctgct
     7081 tatttcgctg aacgctcata agcggtctct ctcccacaag gggagagagt ttattgttat
     7141 atttgtaagt atattttatg caaaccgtct atatcgcctt aggatcaaac cttgagaatc
     7201 cgcttgagca gcttaaacaa gcggtcgaaa aattaaaaaa atttgcaaat gagtttgaaa
     7261 tcagcccgtt ctacggctca aagcctgtcg gaccgcaaga tcagcctgat tatgtcaatg
     7321 cggtagcaaa atttagtacc gacctatcgc ccactgcatt gctggataaa ttgcaaagca
     7381 ttgaaaatga gcagggaaga gtgcgtgtac gccgttgggg ggagagaact ttagatctgg
     7441 atattatttt gtacggcaat cagcaaattc aaaccgagcg tttgaacgtg ccgcatatcg
     7501 agatgaaaaa tcgggaattt gtgattgtgc cgatgtttga tttaacgcct gatttagtac
     7561 taccgacagg ggaaaaatta gccgatattt atgcacaatt taaagatcat caaatgatta
     7621 tcttcagcta atttttcgtt ttgttctcac ttttggtgat caccgctcgg gatttctcgt
     7681 ccatcttcac atcaatgcgg tgatcaacga ccacattcat attaatcgta atgccttcac
     7741 ttggcgtttc aagctgtact ttcggcgtac aagcggtcaa attcagtaaa aatattgcaa
     7801 aataaaatgt ggccggtttc atatttattc cattctttat catcgttcca gtttttgata
     7861 gaggtaattt tctaattttt gttcaaattg tgagccatag ttaattaagt gccacaaatc
     7921 aaacagattt tctcggtgat tatagtttaa attcactttt gatttatttt gattggaaga
     7981 taattttaat tttccgtcta atactaaatc gcctttcggc ccgacattaa tcagactgcg
     8041 aaaatcgtta atcgtgcttt tatctactaa atgtaacaaa atttgctcgg tgtaaccact
     8101 tttctgcatt tctgacaaca attcaggggt aaaggttaaa taggaagttt ccgcctgtgt
     8161 cagtaagcca tcgcaaatat agcaaggttt tccgcttaac caaaacggca agatagcgtt
     8221 ggctttgcct ttgagatcaa tttgctgata ttgcataaat tgcaaaattt gctcaaagtc
     8281 aatccgctct aaattcaggt aagccagttc ggtttgtggt aaggcaaagc gttttacatt
     8341 cagtttaccg ccaaacaaat caaaggataa ttgatttaaa ttcaatggct tagctttggt
     8401 ataagggtag taaccgttga gtttcattga agcgttatct aatgctaaat cccccagttt
     8461 gatttgggca atttttacat caagcggttt tttggctccg aaatgaaagc gtccgttttg
     8521 gtagcggtag ggaagcgaaa attcaatccc ttttgcttcg ccattaggta aagataaacc
     8581 gccgttacga atggctaaat gcccaccggc aatcagcccg tttttactgc cggcactaaa
     8641 cgccgtttcg ccccgaattg taccgttgtt gatcacccat tggctacgaa acggaaaaag
     8701 cggctggaag ccttgagcag attgctccgg ccaataaagt ttgccgataa tttggctggc
     8761 atcttggctt aattggcggc gggcgaacag tttgagtggg ccgagttcgg tggtattcac
     8821 cgctccttta aaacgtagtt tttcgacttc accatcaaag gcgagattca ctcgtggttt
     8881 gggtagcaca ccgccataac tgaaggcaat ctcaggtgat tgcaacatta ccgctccgtc
     8941 aagggtgaat tttttgacat taaatcgaat agcttgcgta ttgttaagcg tgattttaga
     9001 aatggttgta tctgctaact ttacggtggc taaatcgcct ttgagttctt ccaaccgcac
     9061 aagctgttta tgccattgcc ctctgccgct taaattcact ttactgttga aggctttgag
     9121 gatagatgct ccccaaattc gccatttcca actgtctttt actgcgttag catctttagg
     9181 atcttggaaa aaatctaagg ctccagcctt aaaattttgt gcttgtccgt ctaaatggaa
     9241 ctcgatattg ttaaaatcag ggctttgccc tcttaaaatt gcgtgcagtc gtccgtgaat
     9301 accgtgcttg tcaattttaa ttccggcaag tggaaaacgt aaatcttcaa tggttaaata
     9361 ccgctctttt ccggtcaaac gaagtaggga gctaggcaaa aattttacgc ttaaatcgtc
     9421 aaaattacct tgaaaatcca gtggcacgct actgtaaaga ataaaattgc cgtgtttgat
     9481 attacccgtc aaacgaagcg gtaaattcag ccctttttcg gttatttctc cgtctgaatt
     9541 ggagattaca atattgcctt taccccaact gttttgggaa agcaggctga tacgaatcgc
     9601 cgttttgagt ggcaaaaagc cgttttggtt gaatgatttc ggtgtgagct tggtgctaac
     9661 aaacccttgc agtggaaatt gttgggtaaa gtgccaatca aaccgccctt gttgaatttc
     9721 taggctttcc ccatcaaaca gaaaagggaa atccagtttg ttattttcac tttcggcaag
     9781 ttcaacctgc cagttgcctt ctaacttttg tgcggcattt ttttgccaac tgagaataga
     9841 gcgccctttt gcaaggcttt attggcgatg atttcataag ataattgcca atcatattca
     9901 gcggaaatag tgggcggcag ttgggtaaaa ttgtcctcaa tttctgctga aatcctaaga
     9961 ttatgctgtt ctttttcatt cggttgatat gataaatcgg cattcaaccg cccatttgca
    10021 aaatttgctg caaatttgac cgcttgttga gcggcagttg cctgaatttc accttttgaa
    10081 agtgagagtt taatagctgt ttgctcaaaa agccgctgaa gtcttgggtt gaaatcggta
    10141 ggcaaatttt tccagttaag ggcgtggata gtcgcttcgc catcaggaat gaccgccagc
    10201 aatgcgttga gtgaagtgtc ggaattgctg tgttcatttt gtggaaacaa ggcaaggcag
    10261 cggtaatcaa tatccaattt atcgacagaa agatggtttt tctcaatcca acccaaatcg
    10321 gttttatcgg cacttagtag cgggcaatct cgataagaga gttgaaattg agcaagtgat
    10381 gagccttgcc aattcaggtt taagccttgt gggagttgaa ctttccacgc tttcggcaaa
    10441 atccagttaa tcggcgtggc aagatagtgg ctgggcatta aaaaatagta gattgccaca
    10501 acagatagaa cggttgcaat gccgaaaaat tttaatgccc gtttcataaa tcagactaca
    10561 ttagcgaaag ggtataaaat cgctaacagc gtagcaactg tccaagcggt cggatttttg
    10621 atgttttttc caagtaataa ccacgccaac atcaataaaa tcacaaattg tgctgcaaaa
    10681 tagatgataa atgaaagcaa tggcatatcc ggcataattt gtagatgttg ataaagcaca
    10741 aaagctgtcc agcaataatt gagaatcagc accacaaggg cggttttcac caacagtttg
    10801 aggaaaacat ctaaacggct tttcgctaac attaaatacc agccggctaa taaaatgcct
    10861 aacagcactt gggcgaaatt gttggctcga atcccgttaa acaatgaagg cagttcaaac
    10921 caataaatgg ctaaatattg gataatacca atacttgata tagccaaacc acaatacatc
    10981 aatgtgttat ataaggtttg atcttccggc aggcggaagt agattaagca ggcaaaaact
    11041 gcagcaatgc ttgccaccgc cattgggtta tgctttggcg taaagcctat ggaataaagg
    11101 aaattcccta cggcgaaaat aagtaaaaag ggtaaaataa agcttaaacg gctggcttgc
    11161 cctcggcaga tttcgccttt ccatagcact aaaaaactga ttaatgaggt cacgaaaaac
    11221 gccaataagt aaggagaaat aaaaattgaa tttttcggtg cagagtagaa gttaagagcc
    11281 atttcaataa acagcattaa taccattggc agtgcagcgt gggttgaaat ttcaagtttt
    11341 gacggcgaat cgtgatcgtg agccatagat aatcccaaaa ggtataaaga aaatgatttt
    11401 aagccaagat tatgccataa taggggtcgt tttttaactt cagggggaag aaaatatgcg
    11461 aattttacat acaatgttac gagttggaga tttagagcgc tcaattaagt tttataccga
    11521 tgtgttaggt atgcgtctgt tacgtagaag cgagaacgag cagtataagt attcgttagc
    11581 atttttaggc tatgccgatg aaagcgaaag tgcggtgatt gagctgactt ataactgggg
    11641 tgtggataaa tatgagctag gcacggctta tggccatatt gcgttaggcg tagatgatat
    11701 ttacaaaacc attgaagatg tgcgtgcggc aggcggtaaa attacccgtg agccgggccc
    11761 ggtattgggt ggaacaactg tgattgcctt tgctgaagat ccggacggtt ataaaatcga
    11821 atttattgag aataaagatg cgaaagcagc gttaggaaac taatttttgc gaatatgaat
    11881 attcaagcgg tcggatttgt aaaaaatttt gcaaatcaga ccgcttgtat tttaggttga
    11941 tagataaatg gaacagacaa cggaacaaat agattataaa ttattaaagc atcgttttcg
    12001 tggctattta ccggttgtga ttgatgtgga aacagcaggc ttaaatgccc aaactgatgc
    12061 cttattggaa ttggcggcaa ttactttaaa aatggacgag caaggttatc tagtgccgga
    12121 tcaacaatgc cattttcata ttgcgccatt tgaaggggcg aatattaacc ctgagtcatt
    12181 aaaattcaac ggcattgata ttgacaaccc tctaagagga gcggttgcgg agaatattgc
    12241 gattccggaa atgttcaaaa tggttcgcaa agcaatgaag gagcaaggct gccaacgggc
    12301 ggtgattgtt gcccacaatg cagcgttcga tcagggcttt ttacaggctg cgattaaacg
    12361 aattaatgcc aagcgagatc cctttcaccc tttcgcgatg tttgacaccg ccacccttgc
    12421 cggttttatg tatgggcaaa ccgtgttggt aaaagcctgc caggtggcaa aaatcccgtt
    12481 tgacggtaag caagcgcatt ctgcgttgta tgataccgag cgcacggcag aacttttctg
    12541 tgcaatggtc aatcgtttaa aagatttagg tggtttccca ccattatcgg attaacgtta
    12601 ttcatttcat ttattaacac aatttctcgt attgtttcta taaattatcc tgggagtgat
    12661 gatagttagt ggcaagtacc attctgcaat aaatccagcc ctgtagagtt gtagaaaaaa
    12721 taaaaaacta tctatcttca gttaagatta caagcggttg gttttttaac caaatttggc
    12781 aaaaatgtga caaaatagac cgcttgtatt caataacaat agactatttt ttggctataa
    12841 gcactttctg tatacccgct atttcgaccc tccttatttt ttatctaatc taaaaacaag
    12901 cgtttccacc ttgaaatgat taacatgatc ctgaaactaa taaagttcaa acctttacat
    12961 tagtttatat aataaattat aatgattatt attttataaa ttaaaaggag acattatgtt
    13021 taaacttaaa agtagttttg tactgcttaa tgcggcgcta cttgctgctt gttcctcaaa
    13081 tggtggaagc tttgatgttc aatctgccaa agttgaatct caaacgcaaa ctacccccaa
    13141 aaagccaagt ttacaagatg ataatagtaa cgcaagacgt acagtaagcg cttctgaaac
    13201 tgaagcttta ttgcagccgg ggtttggttt ttcagccaaa attccgcgtc gtaatctctt
    13261 tccgcagggg aaggaagatg tagcccctat tggtgatata aaagagatta ctggagatct
    13321 gacaaaaatt ccgtatgaag aagaggttaa agcgtacggt agtagtgctg atggatttag
    13381 ccatactcat gatagaaatc ataagttgta tactagagat tttaattttg ttcgttccgg
    13441 ctatgttctg cattctggtg caaaacctga aataaagcct aaagaaattt tgagaacagg
    13501 tgcacatggg tatgtttact atttaggtat agagccggca aaagcaatac ctactcaaaa
    13561 agctacttat aaaggatatt gggattttac taccgatgcg agaaaaggga gagatagtaa
    13621 atatttttct aattccgcag gcatcaatat tggcgccaca ccggaaaata gttacgatat
    13681 taatgttgat gattctgaaa aaccaatggg gcatacagga gaatttacgg ctgattttgc
    13741 taataaaact ttaactggaa cattggttcg taatgggtat gttagtcgta gcaaagagca
    13801 aaaaattaca acaatttacg atattgatgc aaaaattaaa ggtaatcgct tttctggtaa
    13861 agcaaacgca aaaaataccg atgatcctta ttttggtaaa agctctacga cacttgaagg
    13921 tggatttttt ggtgggaagg ctcaagaact tgccggtaaa ttcttagctg atgataagtc
    13981 ggtatttgtt gtttttgctg gcaaacgaga tgctaaaaaa gatgatagtg aatctgcctt
    14041 tgatgctttc aaaattaaac ttaaagattt aaataaatct gagatggata ctttcgggaa
    14101 tgcgacacat ttgattatta acaataagca gattccactt attgcggaag gcaaaaaaag
    14161 ctttgccgag atgaaatttg atgatttggt tacccgtact attgatggta aaacgtatcg
    14221 agtttcagtc tgctgtaata atttagatta tgtcaaattt gggacttata gcgagggaaa
    14281 taatagtgat actgatactg ctcaccaata tttagtagga gaacgtacag ctgtggcaga
    14341 tttgccaaca gggacagtaa aatatcgagg tacttgggac ggggtaatgt acagtaaatc
    14401 tggctcggca ggggctgaat cgccaagtaa cagcgaaagt ggtactcgtt cactattcga
    14461 tgtagatttt gtcaataaaa aaattaatgg caagctgatt gctaatgatg gtgttgaaga
    14521 acgcccaatg ctgacacttg aaggcaatct gaaagggaat ggttttgcag gcacagccaa
    14581 aacgggcaat tctggtttta atcttgatcc gaaaagtacg aatggtggca cggtagtgca
    14641 tataaatact caatttgaag ggggctttta tggccctaag gcgacggaat taggtggtat
    14701 tgtacatagt gcagaaacgg ataaagatag agtcagtatt acattcggcg gaaaacgtca
    14761 aatagaaaaa taatcataat tcccctttgc tggttgtaga tagcaagcgg gcaatttttt
    14821 ataaaaattt gcaaaattta aataaaggag accctatcta atgataatga aatatcatca
    14881 ttttcgctat tcacctgttg ccttaacagt gttatttgct ctttctcatt catacggtgc
    14941 tgcgactgaa aataaaaaaa tagaagaaaa taacgatcta gctgttctgg atgaagttat
    15001 tgtgacagag agccattatg ctcacgaacg tcaaaacgaa gtaactggct tggggaaagt
    15061 agtgaaaaat tatcacgaaa tgagtaaaaa tcaaattctt ggtattcgtg atttaactcg
    15121 ctatgaccct ggtatttcgg tggtggaaca aggtcgcggt gcaagtagtg gctatgccat
    15181 tcgaggtgta gataaaaacc gtgtcagctt acttgttgat gggctaccac aagcgcagca
    15241 ttatcatacg ctaggttcag atgctaatgg tggtgcaatt aatgagattg agtatgaaaa
    15301 cattcgttca attgagttaa gcaaaggagc aagttctgcg gaatatggct ctggtgcgct
    15361 aggtggtgct attggttttc gtactaaaga tgcgcaggat attattaaag aggggcagca
    15421 ttggggctta gatagtaaga cctcttatgc cagcaaaaat agccattttt tacagtctat
    15481 cgcagcggct ggtgaggcgg gtggttttga agcacttgtt attgcaactc accgacacgg
    15541 taaagagacc aaaattcatt ccgaggcaaa taaattaaaa cataatattc ggcgtataac
    15601 cggctttgaa aatcgctacg atctttaccc aaattccgca cagaatgctc ctggaggatc
    15661 tttttttatt gtggaagata cttgcccaac attagattgt actcctcgtg caagggttaa
    15721 gttgaaccgc gataatttcc cagtgagaac atttccggaa tatacgcctg aagagcgcaa
    15781 acagcttgag cagattcctt atcgcactga gcagctctca gcccaagaat ataccggtaa
    15841 agatcgcatt gcaccaaacc ctttagatta caagagtaat tctgttttta tgaagtttgg
    15901 ctatcacttt aactcgtctc attatcttgg cgcaatctta gaagatacaa aacaacgcta
    15961 cgatatccgt gatatgcaaa cgccagctta ctatacaaaa gacgatatta acttatcact
    16021 taggaactat gtttatgaag gggataatat tttagatggc ttagtgttca agccaaggat
    16081 cccttatggg ttgcgctata gccatgtgaa gttttttgat gaacgtcacc acaaacgtcg
    16141 tttaggattc acctataaat ataaaccaga gaataatcgc tggttggata gcattaaact
    16201 cagtgcggat aaacaagata ttgaactata tagccggcta catcgcttgc attgtagcga
    16261 ttatcctgtg gtagataaaa attgccgccc gactttggat aaatcttggt ctatgtatcg
    16321 aactgagcgt aataattacc aagaaaagca tcgtgtcatt catttagaat ttgataaagc
    16381 gctaaatgct ggtcaaggcg tatttaagca aacccacaaa ctgaatttag ggttgggctt
    16441 tgatcgattt aattcgctta tggatcatgg ggatatgact gcccaatata ccaaaggcgg
    16501 ttataccagc taccgcggta gagggcgttt agataatcca tatatttatc gccgcgatcc
    16561 acgcagtatt gaaacggtat ctttgtgtaa taatacacgc ggcgacatct taaactgtga
    16621 accgcgtaaa attaaaggcg atagccattt tgttagcttc cgcgatctag tgataagcga
    16681 gtatgtggat ttgggattag gggtgcgttt tgatcaacat cgatttaaat ctgatgatcc
    16741 gtggacactt agccgaactt atcgaaattg gtcttggaat ggtgggatta cgcttaaacc
    16801 aacagagttt gtatcgcttt cttatcgcat ttcaaacggt tttagagtgc ctgcattcta
    16861 tgaactttat ggtaaacgta atcatattgg gcttaaagat aacgaatatg tgcaacgcgc
    16921 gcaacgtagc caccagttag agccagaaaa atcgactaat catgagattg gagttagctt
    16981 taaaggtcaa tttggttacc ttgatgtgag ctatttccgt aataactata aaaatatgat
    17041 tgcgacagca tgtaaaagaa taatacaaaa atcacactgt ttctataact accataatat
    17101 tcaagatgta gcactaaacg ggataaattt agtcgctaaa tttgacttac acggtatttt
    17161 atctatgctg ccagatggtt tttattcatc agttgcttat aaccgtgtaa aagtaaaaga
    17221 gcggaaacta accgactcaa gactcgatag cgtaaacgat cctattctag atgcgattca
    17281 gccagcacgc tatgtgcttg gattcggcta cgatcaccca gaagaaaaat ggggaattgg
    17341 cattactacc acctattcta aagccaaaaa cgccgatgag gtggcaggca cacgtcatca
    17401 cggcatacat cgcgttgatt taggtggcaa actgaccggt tcttggtaca cccatgatat
    17461 taccggttac atcaattata aaaactacac cttacgtgga ggaatttata atgtgactaa
    17521 tcgtaaatat tccacttggg aatcagtgcg ccaatccggt gtgaatgcag taaaccaaga
    17581 ccggggtagc aattacactc gatttgccgc tccggggaga aatttcagtt tagcatttga
    17641 aatgaagttt tagacggcaa aataaaatag aaaaacgctg aatagtatat tcagcgtttt
    17701 ttgttggctc taactaatta catacgaatt aaaatcgtgt tttaacggtc ggttagatta
    17761 acccataccg tattttttta attttttgcg taatgtgcca cggttaatgc ctagcattgt
    17821 tgctgcacga gtttgattgc cgcgagtata ttgcatcacc atatctaaca ttgggtgttc
    17881 gatttcagat aatactaatt cgtataattc tgttggatct tcaccattta attgagataa
    17941 atagtttttt aatgctgctt tcacattatc gcgaagtggt ttattaacct gctgcgcttg
    18001 tgcatttaac attgttactg ttaatgggtt ttgtgtaggt tgttgctcta acattttttt
    18061 atccaacttt tcttaattga gattctaaat gaatcgattt aacaaaatct tctaatgcat
    18121 aaagttgctc ttttgtggaa cttagtgtat taaaagtgcg tttaaaattt gattccggat
    18181 ttaatttttc ggtataccaa ccaacatgtt ttcgtgcaat acgataacct ttttcttcac
    18241 cataaaattg gtgcaaatcc tcaatatgtt taaacattaa ttggtatttt ttatcaattg
    18301 ataatggtga agataatcca gtttcaaaaa aatcattaat ttccttaaat atccaaggat
    18361 ttccaaagct agcgcgccca atcatgattg catcggcttt ggtatagtcg agaacaaact
    18421 ttgctttttc cgcagagtca atgtctccgt ttgctatcac tgggatagaa attgcttctt
    18481 ttactgcctt aatactttca tattcagctt cgccattaaa taaacagctt cgagtacgcc
    18541 cgtgaatagt gagagctttt atgccggctt gttctgcaat tttcgcaatt tgcagacaat
    18601 ttctattttc ctgatcccaa cctgttctaa tttttagtgt cacagggaca tcaacggcat
    18661 tgactacaga atctaatatt tgagcgacta atttggggtt acgtaaaaga gcagagcctg
    18721 ccatattttt attcaccttt tttgccgggc aacccatatt aatatcaata atatcggcac
    18781 catactcaac atttatcttt gcagcctttg ccatttcaac aggatcagaa cctgcaattt
    18841 gtacggcatt taagcctatt tcatcgtggt gtgctaaccg caatcgtgat ttctcagtat
    18901 gccatacatc aggattggta gacatcatct ctgaaaatgt taaacctgct ccaagttggc
    18961 tacataagcg gcgaaaaggc tgatctgtga ttccagccat aggtgcaagg aaaatacgat
    19021 ttttgattgc atattgtcct atttgcattt ttaacctttt attaacaaat ttgaagtaat
    19081 tttaaccaga gggatatcca aataaaaagt acggtaaata ccgtactttc ttataatagc
    19141 ttatggatca gtttagtact gatttgaggg ctggtatgat acgcattttt taggtatttt
    19201 ggaagtctat aaattcgaca atttataaaa aaattgaaaa tttttatatt gacattctaa
    19261 aaatgaaaga attaagtatg ctttactcga attagctcag cttttgtctt tacccaatta
    19321 atttctgcta tggttgaagt tggaaattgt acattgcttt ggttattcgt taaaatttgg
    19381 acgagatcga acacaagtgg gagatgtgaa atcagaagaa tattttttgc gccttcctct
    19441 cttaaaaaat gtagatagct ttctactgta tgcgggtttc cgtcaggcgt aacctcgtcc
    19501 caactttcaa tgaggttggt aaaatgttgg ggaaaattga ccgcttgcat accaataagc
    19561 acattttcaa gcgtttgttg cgccctaagg tatgggctga cgagaatttt atctaattga
    19621 atttgttggt tggctaaatg ttctcctaac cattttccct gtgaaatggt gttttttata
    19681 ccattgtcgg ttaaacagcg agcagcatca ctcgaagcat taaagcctgc ttccccatgg
    19741 cgcataatcc aaatattcat aacattctcc tttagttgta tttaaactct cttgatttag
    19801 gcgtataatg accctcgttt aacgcataat caagatgctt agaaagattt taacttaatt
    19861 tttaaattta ttgatggagt caaataatgt ctagaaaact cagaagaacg aagatcgttt
    19921 gtacaatggg acctgcaaca gatcgtggca atacgttgga aaaaatcatt gctgccggtg
    19981 caaatatggt tcgtatgaat ttctctcacg gtattcctga agatcacatc gaacgtgcga
    20041 ataaagtgcg tgaaattgct gcaagattag gtcgccacgt tgcgatttta ggcgatttgc
    20101 aaggtcctaa aattcgtgtt tcgacattta aagacggtaa aattttctta aatattggtg
    20161 ataaattcac cttagacgca gatttaccgc gtggagaagg ccatcaagaa gctgtaggtt
    20221 tagattacaa aaacttacca aatgatgtag tacctggtga tattctattg ttagatgacg
    20281 gtaatgttca actgaaagtg ttggaagtgg aaggcgttaa agttcatact gaagtgacag
    20341 ttggtggccc gttatcaaac aataaaggga ttaacaaatt aggtggtggt ttatcagctc
    20401 ctgcattaac cgacaaagat aaagaagaca ttaaattagc ggcgaaaatc ggtgtggatt
    20461 acctagcggt ttcattccct caatcaagtg cagatttaca ttatgcacgt gaattagcaa
    20521 aagaagcagg cttagaagcg aaaattgtag cgaaggttga acgtgccgaa actgttgcga
    20581 ctgaagaagc aatggacgac atcattttag gtgcggacgt tgtgatggtt gctcgtggtg
    20641 acttaggggt tgaaattggt gatgcggctt tagtgggtgt gcaaaaacgt ttaatccgcc
    20701 gtgctcgtaa gctaaatcgt gtggtaatta ccgcaaccca aatgatggaa tcgatgatta
    20761 aaaaaccaat gccgacccgt gctgaagtga tggatgttgc aaatgctgtg ttagatggta
    20821 ctgatgcggt aatgctttcc ggcgaaactg caaacggtga ctaccctgtt gaaacggtaa
    20881 aatcaatggc agaagtgtgc ttaggtgctg aaacaatgcc aagcattaac atttctcgcc
    20941 accgtatgga aggtacgttt aaatctatcg aagaagctgt ggcaatgtct gcaatgtaca
    21001 ccgcaaacca tttagaaggt gtatcggcaa ttatttcatt aactcattca ggcgaaacgg
    21061 ctaaattaac cagccgtatc agctcaggtt taccgattta tgcgatgtct cgtaaccaaa
    21121 aagcattaaa cctttgtgcg ttatatcgtg gtgtaacacc ggtatattac aatgaagaca
    21181 gccgcacaat ggaaggggcg aaaaaagcca ttgctcaatt aaaagaacaa ggctatttat
    21241 taaccggcga attagtgctt ttaactcacg gagatgaatt aaaagctggc ggtacaaata
    21301 cttgtcgtat cttaagagtt gaataatcta aacgattaac gcaaaagcca cataaaacac
    21361 tttatgtggc tttttttctg tcttaatata gcttaatgta atctgaacta ttgatattat
    21421 tatgcacaat aaacttcttt ctatctgtat tctccgcctt tctgccattg gcgacgtttg
    21481 ccatacctta gcggttattc aagctattca acgaaaatat cctagtgctg aaattacttg
    21541 gattatcggc aaaacggaag cgtatttaat gaaagatatt ccgaatgttc acttaattcc
    21601 ttatgataaa aaaatagggt ggaaaggtat ctttacactt tggcaaaaac tttctcacaa
    21661 gcggtttgat tttttattaa atttacaaac ggcattccga gcttcaattc tatcgcttgg
    21721 gattaaagca aagaaaaaaa tcggctttaa tcaagacaga gcaagggaaa tgcaatggtt
    21781 gtttaccaac cagaaagtag aaatgacaga ctctcctcac gtgcttgacg ggcaaatgat
    21841 gtttgccaaa gccattggga taatggattt atcggcacaa tggaatttac ctgtgagtga
    21901 tgaagattta gcttatggca aacaatttat tgattttaca aggaaaaata tactcattgc
    21961 tccttgttca agcaagaaag agaaagattg gttgccggaa cgctatgcag aaattgctaa
    22021 ctggctaagt aagcaaaata tcaatgtatt aattgcaggt tctccatcgc aatatgaaat
    22081 ggaaaccgca gccaaaattc aacaacttgc cccgaattgt acgagtattg ccggtaaaac
    22141 cacgctaaaa cagcttgcag gtgttattcg tcaggttgat ttggtgatct cttcagattc
    22201 aggtcctgcc catatcgcca ctacgcaaaa tacgccggtg attgggttgt atgcgattca
    22261 taatccaaga cggacaggcc cttacaatga cttagacaaa gtggtttcgg tatatgatga
    22321 agcgattttg caatcttatg gtaaaccttg gcagcaatta gcttgggcaa ctaaagctaa
    22381 aggcaaaaat tggatggaaa aaatcacggt ggagagtgtg aaacaaaaag tagttgaaac
    22441 tttaaaaatt acgttgtaaa gtttgtgtat taaaagattt acacaaaagg aaattaaaat
    22501 gaacacagtg tttaatcaag atagcgtgca taacgtaaat atccaagatg aaaaggtatt
    22561 actgacccca caagagttaa aacaagaatt tccgttgccg gaacatttac gcaatcaaat
    22621 tgaacgttca cgcaaaacga tttccgatat tattcacaga cgagataaac gccaactgat
    22681 tgtgattgga ccttgttcga ttcacgaccc agttgctgcc cttgaatatg gtaaaaaatt
    22741 aaaagctctt tcggagcaag tgagtgataa gttgtatatt gtgatgcggg tttattttga
    22801 aaagccccgc actactgtag gttggaaagg gctgattaac gatccgaaaa tggacggctc
    22861 ttttgatgtc gaaaccggct tacgtatcgc acgaaaactc tgtttggatt tagccgaact
    22921 tggcttgccg ttagcgaccg aagccttaga tccgatgaca ccacaatatt tagcggattt
    22981 gttcagctgg tcggcaattg gagcaagaac aactgagtct caaactcacc gtgaattagc
    23041 ctcaggtttg tcaatggcgg taggctttaa aaatggtaca gatggtgatt tagccgttgc
    23101 acttaatgcg atgcaatctt ccgctcaaag ccatagcttt atcggcatta accaacaggg
    23161 gcaagttact ttactcaaaa caaaaggtaa tgcagacgga catattattt tacgtggcgg
    23221 taaaacccca aattatgaaa agcaatatgt tgatgattgt gaatgggtgc taaaacaagc
    23281 aaacttacct acagcgatta tgattgattg cagccacggc aattccaata aagattaccg
    23341 ccgccaaccg ctggtggcag aaaatgtatt agagcagcta cttgctggca atacttcgat
    23401 tatcggttta atgattgaaa gcaatttaca cgcaggtaat caatcatcag aattgccgtt
    23461 cgagcagatg aaatacggtg tttctattac ggatgcttgt attgactggc aaactacaga
    23521 agagctttta actcactttg ctgaaaagtt gcgttaaaga taatttgcaa aaaattgcat
    23581 aaaaacgacc gcttgtaaat tggtaacaaa tatgaaccca ttagccccat tacgagaaca
    23641 aattgatcaa gttgatcaac aacttatcgc cttactttct gaacgcttag ctcttgtggc
    23701 agaagttggg aaagtcaaat cagaacacgg cattcctgtt tatgctccgg agcgagaaac
    23761 cgcaatgatt tccgcccgcc gctctgaagc ggaaaaacaa ggtattccgg cggatctcat
    23821 tgaagatgtg ctacgccgta taatgcgtga atcttatgcc aatgaaaaca aacacggctt
    23881 caaaagagtt aattcggaca ttcaaaaaat cgtgattgtt ggtggtaaag ggaaattagg
    23941 tgggctgttt ggcaattatt taaccctatc cggctataat gttgaggcat taggacgtga
    24001 tgattgggac aatgccgagc agattttagc caatgcagat gtggttattg tgtgtgtgcc
    24061 gattgccaac accctagcaa ccatcgagcg tttacaatct tatttaaccg aaaatatgat
    24121 tttggcggat ttaacctccg tcaaacatca gccgttgcaa aaaatgctgg aagtgcataa
    24181 tggagcggta gtcggtttac acccgatgtt cggaccggat attgcaagtc aggcaaagca
    24241 agtggtcgct tgctgccacg ggcgtttccc tgaacgttat caatggttgc ttgagcaaat
    24301 caaaatttgg ggggcaaaaa ttgaagagat tgaggcagca gcacacgata atgcaatgac
    24361 ctacattcaa gccctacgcc acttttccac ttttgcattc gggctacatc tttctcgcca
    24421 gccggtgcaa ctctctcaat tattggcact atcttcgccg atttaccgct tagagttggc
    24481 aatgattggg cgtttatttg cccaagacgg cagcttatat gccgatatta ttgccgataa
    24541 accggaaaat ttagccacca tcgaaacctt aaaagagagt tttgaacaag ggctagattt
    24601 ctttaaacgc aacgacaaag ccggttttat caaggctttt gaggaagtgc atcactggtt
    24661 tggcgattat tccgagcagt tcttgaaaga gagtagagta ttgctccaac aggctcacga
    24721 ttcaagaaag taaaggtagg ttgaggtttt gctctgatag cactagcgtc cacgctagtg
    24781 ctatttaata ttctacaagc ggttaataaa gagctgtttt ttgcaaattt ttatgcaaaa
    24841 ttatccgctt gttttggcac gagcgtggac gctcgcgcca tcggaagaaa catttttgta
    24901 aaattgcccc caaatctgac cgcttactgt ggctgaattt ctccattttt taccgtaaac
    24961 aaacaatcat cgcttctgtc ttgccattgc atttgattga gctgatcttg agtaatggcg
    25021 gtaacgaata cttgtgagcc actttctctt aaacgatgtg ctaaaagctc acgtttggtt
    25081 gggtcgagtt cggaggcaaa atcgtcaatt aaaaataggc attggcgttg tttttgggcg
    25141 accaaatgtt cgccttgagc cagtcttaac gcacacatca atagctttaa ttgcccacgg
    25201 gaaagcacat cttccaccgg taagccgttc gctttaaaac ggaaatcggc tttttgagct
    25261 ccaatcatcg tatagccaag agctttatcc cgctcaaaac cttgtgccag aatctcggca
    25321 taatccgccc ctttttccca gccttgatga aagctcaagc cgatttcgat ttccggcagg
    25381 aaaaattggc aggttttctc aatttcgggg cgtaacgctt cggcgtattc ggctcgcatt
    25441 tcgctcacgg cataagtggt tttcaccaat tcgatatccc acgctttgag ttcttgataa
    25501 gagcggactt gttgcaaagc cgaattacgt tgctttaata aacggcgaag attgttccaa
    25561 tgagcgtaga aatcagggtg ctggtgaaat aagccccagt ccaaaaaagc acggcgaaag
    25621 ctcggaccac cgttaagtaa ggttaagcct tcgggggtga tcacttgcat cggcaataaa
    25681 tgggctaaat cggcaatttt gttgccgtct tcaccgttga ttttaagtgt cgtctcgcca
    25741 ctgcgaaatt tctgaatgcc gaccgaccaa ctgtgctttg cctcgtcaat tttaccgtgt
    25801 agtacgaaat cgtctttatc gtaatggata atgcggttgc tgatatggct tttgaatgaa
    25861 cgcccgtgtc caagatagaa aattgattcc aacaaactgg ttttgccact tccgttcgcc
    25921 ccgactaaaa aattaaaatg cgggctaaat gctaaatcgg tatgttggat atttctaaaa
    25981 tggttgatta ataaacgggt taaagccata gttaaagtgc gaggaataaa aagagtagcc
    26041 attttagcaa atttgcaaaa ttttgggaaa aattaaccgc ttgcggatta gaaagtggtt
    26101 cagtagaata agccgtttat tttttattga aaattaagag gaaaaattat gtttggaaaa
    26161 ggtggcttag gcggcttaat gaaacaggct cagcaaatgc aagagcgtat gcaaaaaatg
    26221 caagaagaga tcgcccaatt agaagtaaca ggcgagtctg gtgcgggttt ggtaaaagtc
    26281 actattaacg gggctcacaa ttgccgccga attgaaattg atccatcatt aatggaagat
    26341 gataaagaga tggtggaaga tttagttgcc gctgccttta acgatgcggt tcgccgtgca
    26401 gaagaaatgc aaaaagagaa aatggcaagt gtgactgccg gtatgcaact gccgccgggt
    26461 atgaaattcc cattctaatt taagtatgca aaccagtcct ttacttgaaa atttgatgga
    26521 agccttgcgt gcattgcccg gtgtcggacc gaaatcggca cagcgaatgg cttaccatct
    26581 gttgcaacgt aatcgttccg gcggtgtggc gttgtcaaaa gccttaaatg aggcaatgag
    26641 ccatatcgga cattgcaaat cgtgccgtac ttttaccgaa gaggaagagt gtacgatttg
    26701 taaaaatcct cgccgtcaga tgagcgggca actttgtgtg gtagaaatgc cggaagacat
    26761 tcaagcgatt gagcaaaccg ggcaattttc agggcgttat tttgtgctga tggggcattt
    26821 gtcgccgatt gatggtatag gccctcgtga aatcggctta gatttattgc aacaacgctt
    26881 ggaacaggaa tctttccacg aagtgatttt ggcgactaat ccgaccattg aaggtgatgc
    26941 taccgcaaac tacattgctg aaatgtgcag aatgtataac gtgaaagcta ctcgtattgc
    27001 tcacggtgtg ccggtcggtg gttcattaga aatggttgat ggcacaacgc tttctcactc
    27061 ttttgcagga cggcgtgata ttgatattta aag
//
LOCUS       DANLYI010000010        24543 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.010, whole genome shotgun sequence.
ACCESSION   DANLYI010000010 DANLYI010000000
VERSION     DANLYI010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 24543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24543
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.010"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            complement(<1..725)
                     /locus_tag="PXD81_000325"
     CDS             complement(<1..725)
                     /locus_tag="PXD81_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5536394.1"
                     /translation="MKNNKTDTLLFLEQFARYLTAKSWQIAFELEYAVIWQKDLGHRI
                     LEVTLPKPIAEDREEVLQKALEKLAKIEEKNVETLKLNISNQYTDKLSVRVIGESVKD
                     GTIPLNEGVKLFEKTKHLINALALSAKKKKAAFGHNSGGKDVAEFMSQVRLGQTQIGS
                     YIVNLSYPVENIEIKEQNEIIQAISFSRSVSHNLVNSLNKLKEKITHYDHNPTIFAEL
                     IPEGVSHNLCEAIIGLSGSDQQR"
     gene            complement(722..1225)
                     /locus_tag="PXD81_000326"
     CDS             complement(722..1225)
                     /locus_tag="PXD81_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4365 domain-containing protein"
                     /protein_id="HDL5536395.1"
                     /translation="MLSPTQQMEQFNIAYVLAITANAGFNHAVPVVDNHSVDLAISAE
                     FPTEEGKRSDPEIKLQLKSEGDLHIKNGLVSYKLKKKNYDDLRKNCANPRYLIVCDLP
                     KKTTQWLSHKKKFMTLKRHCYWVSLKGFPATTNRSSVTLTFPENQRFTTDVLIKMIEY
                     ARVGETL"
     gene            complement(1230..1961)
                     /locus_tag="PXD81_000327"
     CDS             complement(1230..1961)
                     /locus_tag="PXD81_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL5536396.1"
                     /translation="MRPLKEIRYDNLLRLIDEAKSTSDLANRTGIAVSYLLQIKNKNA
                     IQNGKPKGIGDKIAAKLEDGMNKPRGWLDQIHSEQSNLINSRIGNEEQKLQEQDLNDF
                     IIIDVLDVSASAGFGSSSELVEVVNQMRYVPEQFYSLFRNMAPRYIRIINLSGDSMYP
                     TFSSGDMLFVDISVNEFTGDGVYVFTYKGHLYVKRLQNTGDQILVISDNKLYEKWSIT
                     EENQDQLFIHARVKVHQSQQLNFIG"
     gene            2088..2312
                     /locus_tag="PXD81_000328"
     CDS             2088..2312
                     /locus_tag="PXD81_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL5536397.1"
                     /translation="MQLQEYIEKSKWGEISRLAKAIDVAIPIVSFWVNGKRQVPAKRC
                     PEIEKFTEGKVTCEELRPDVDWAVLRNSGK"
     gene            2361..3122
                     /locus_tag="PXD81_000329"
     CDS             2361..3122
                     /locus_tag="PXD81_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HDL5536398.1"
                     /translation="MKSQSLLPITAQNASGAEVKISSIEIAELCEKQHKNVIVDIRKM
                     LAELKLNSADFSAQYQTKDGRMQPCYFLPKRETMILVSGYRIDLRAKIIDRLDELEKQ
                     HQPSIPKNYPEALRMAAELAEQNQVLLLEKQQTTAENHALKSYFEPGLTPAQFVKGLN
                     GVNSNKINDYLRSRGWLYKDKHCWRVFSHVRDKYLTESFKRIEVDPVERIEMATYKPI
                     LLEKGAVKIFEAYLQGKLPMKADWNGKYYHSKVAV"
     gene            3119..3910
                     /locus_tag="PXD81_000330"
     CDS             3119..3910
                     /locus_tag="PXD81_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL5536399.1"
                     /translation="MSMLLMVKAMNCTVGNSARKLVLLKLADNANDDGVCFPSYQFIA
                     DKCEMSKRSAISHIDELIKMGYVSKKSRKNKDGSSSNLYQLHLDAPSEKSALGGENFA
                     PGGSENSAPITSHSINQSINHNTSEQNSDGVDKKSKYRFSDNDMAVAKWIFKRVKQLA
                     PQTKESYLPNWANEVRLMVERDKHTHQEICSLFKFATEHHFWGRNILSVVSLRAKWDR
                     LNLERQSSLRPNNIHSATGNGDIQDTTGFSKGLVVDVGGGTKWEF"
     gene            3898..4533
                     /locus_tag="PXD81_000331"
     CDS             3898..4533
                     /locus_tag="PXD81_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HDL5536400.1"
                     /translation="MGILATTTQNQVLPVEIVEQAEVETTKIFNRLKATHLGWRANIK
                     SQAEYDNARREWVRLLIENRLTPKEVERGLKIADTDKNPYLPSFGQFLEWCKSIDYAE
                     LGLPDMDQFLKRLNHFSAFGFTEIDNFKFRSNAEYWLLTDLYERNRKHSWKDETLRNQ
                     AEKALVEMAKRIMSGEQIPPPKPVIEEKKKVPIDPRIQRILDEKKQAGAMQ"
     gene            4530..5102
                     /locus_tag="PXD81_000332"
     CDS             4530..5102
                     /locus_tag="PXD81_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine methyltransferase"
                     /protein_id="HDL5536401.1"
                     /translation="MTDFDKDTYQTPNYVRNWLNHRYAWFHIDGCSNGKNALYSHWIG
                     KAAEGLDEDTLSGQIAEDFLAENLLDVILERVADWGELLRIFVNPPYSNPLPFVQRAA
                     ELKKAGHIVVMLLPADKSTRWYQVIQENASEVIDIIGGRINFINPLTGEEVKGNNKGS
                     MVAVFDPFVEGIVTRQMPLDKIKELGGYEK"
     gene            5221..5673
                     /locus_tag="PXD81_000333"
     CDS             5221..5673
                     /locus_tag="PXD81_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011609553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HDL5536402.1"
                     /translation="MAKYQMMKLPGGILSPLNDEEAERLKSFQNGEQYEIEIKRQRNP
                     AFHRKVFAFFNFCFDCWSGSHSDWEFQDPTAQFDTFRKHLTVMAGFYVQTWNLKGQVR
                     IEAKSLSYANMEQDEFEQAYKALINAAIKHVFNNTKDENTINQLYSFF"
     gene            5723..5935
                     /locus_tag="PXD81_000334"
     CDS             5723..5935
                     /locus_tag="PXD81_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5536403.1"
                     /translation="MKKNVIRYWLSVPFRIIAVLISLSILTLRYIGYLLIYPLAFFVA
                     VGSFIVIGQWKYQECLDDFKSDFNPI"
     gene            6312..6596
                     /locus_tag="PXD81_000335"
     CDS             6312..6596
                     /locus_tag="PXD81_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005672808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HDL5536404.1"
                     /translation="MANLRKEAKGRECQVRLPGICNHNPETVVLAHYRMAGLNGVGMK
                     PDDIFGAWACSSCHDECDRRTRKLEADYVRQAHAEGVFRTQAILRREGKL"
     gene            6593..6961
                     /locus_tag="PXD81_000336"
     CDS             6593..6961
                     /locus_tag="PXD81_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005613578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HDL5536405.1"
                     /translation="MSDWLEIALPYPPSVNHYWKHTRNGRHYISDKGKAFRAEVVKIC
                     KMFDPFKGAVQTVLQIYYPDKRKRDPDNLEKALWDALKIGGLIEEDDNQILVDKRTIT
                     AGFKKGGMVVLKIKAVGNAN"
     gene            6951..7316
                     /locus_tag="PXD81_000337"
     CDS             6951..7316
                     /locus_tag="PXD81_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5536406.1"
                     /translation="MQIDVKGVLNLWGGYVRFTDCKGYPTMQAFMRESPQERTKQRYL
                     ARLDDDTLEKVDRQMRKLQANDKRQFDVLFYKYAVGLENKQVWQKLSLSKTSYHTELL
                     LAERFMEGALAGSEIRLLL"
     gene            complement(7284..7478)
                     /locus_tag="PXD81_000338"
     CDS             complement(7284..7478)
                     /locus_tag="PXD81_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005714695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5536407.1"
                     /translation="MALSRTEIVNRSNAKRGIKLKAFKLHESTIAQIEQLAQQLNIPQ
                     NQLIKLAVEKLQEQTNFTTG"
     gene            7905..7980
                     /locus_tag="PXD81_000339"
     tRNA            7905..7980
                     /locus_tag="PXD81_000339"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7938..7940,aa:Trp,seq:cca)
     gene            complement(7995..8573)
                     /locus_tag="PXD81_000340"
     CDS             complement(7995..8573)
                     /locus_tag="PXD81_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5536408.1"
                     /translation="MIEFDQIDDSFYHFPFRPRPGDTSSDTATPCIGGVDLVSNPEKV
                     ELIPEAAHSPMLKKLLTDLNTPNSKLLTLGCAYWAHKDNRDTSYAYLEFSFREHSVAT
                     NLEFIRSIDEQFEQFLQENKKQLSIEFSVPEQAFDIVSQALFWSIRPFSYFGSEERIL
                     IYFQAGSPRHQDLEIFLDLLHRFLTEYLVVPA"
     gene            8692..9036
                     /locus_tag="PXD81_000341"
     CDS             8692..9036
                     /locus_tag="PXD81_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin, lambda family"
                     /protein_id="HDL5536409.1"
                     /translation="MPEKNPDIYTQAWNYLVMFLSNHNGWICGVIIAFFTSITKSFLY
                     GKTDTPKRVLAEAILCSVIAGSMRPILIYLNADVSLITPIGAGIGLLGTSAIRQMILK
                     FLNKKAGGENVD"
     gene            9026..9619
                     /locus_tag="PXD81_000342"
     CDS             9026..9619
                     /locus_tag="PXD81_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 19 protein"
                     /protein_id="HDL5536410.1"
                     /translation="MWISESKFKQVFPNAVSGIYSIIDKHIEKAGCKTKEQQAMFLAQ
                     CGVECAGYSVFEENLNYSATALLRTFPKYFSQHNVVLYARDKRAIANRVYANRMGNGN
                     EQSGDGWKYRGRGLIQITGKANYIEFAKWEGLPSLVTDPDQITKNLALTVQVAVWYWV
                     SRKLSEFSDILTVTKKINGGTHGLTQRTAYYKKLMAD"
     gene            9620..9949
                     /locus_tag="PXD81_000343"
     CDS             9620..9949
                     /locus_tag="PXD81_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2570 domain-containing protein"
                     /protein_id="HDL5536411.1"
                     /translation="MIKYIYLAATVMILGLCGWIWHQSKQIDGLRAENQTQAQTIEQQ
                     QKANQRLTDSLEQERQAVEKIQKIANELRNKVEMAKNEITSILAQDSCAKADLPNGVA
                     DSIKRLH"
     gene            9915..10148
                     /locus_tag="PXD81_000344"
     CDS             9915..10148
                     /locus_tag="PXD81_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5536412.1"
                     /translation="MVLLIALSGCTSKTIVSTEYLYPPAAYLVPCERTAFSGKTYGDT
                     VDYLIKVMGERDLCASQIDRIREWQAQTKQGFK"
     gene            10415..10891
                     /locus_tag="PXD81_000345"
     CDS             10415..10891
                     /locus_tag="PXD81_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1441 family protein"
                     /protein_id="HDL5536413.1"
                     /translation="MDNLFDLKLNINQIAELTGLHRQTVSQRVAGLNPALGSNSKLKL
                     YALRDLILTGLAEKMSADVDSLSPADRRAWFQSENERLKFEKEIGELIPASEVALEMS
                     ALAKTVVQALETLPDILERDCGLQPKDLIRVQQVTDDVRDQMALHIQEVTTDTENE"
     gene            10891..13002
                     /locus_tag="PXD81_000346"
     CDS             10891..13002
                     /locus_tag="PXD81_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010786315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HDL5536414.1"
                     /translation="MFASAKEIRRDIANAIKAPRRMRVSEAVAEYMRVPLGGGNSVKW
                     DKHTAAYMLEPMDCLNSREYDAVIFVGPARTGKTIGLIDGWITYSIICDPSDFLLVQL
                     TQEKASEHSRKRLDRTFRCSPEIAKRLSPRKNDNNVHDKYFRAGNLLKIGWPSINVLS
                     SSDYKYVALTDYDRWPEDVDGEGDGFSLASKRTTTFMSAGMTLVESSPGKDIVDLKHI
                     PKSTHEAPPTTGILSLYNRGDRRRFYWQCPDCSEYFEPSMANMVGYREDSDFVKASEN
                     ARLQCPHCQTLIAPELKRELNIKGVWLKEGQKIDASGQISGESRKSRIASFWLEGPAA
                     AYQTWAQLTYKLLNAEHEYEMTGSEETLKAVTNTDWGLPYLPRSALEQRRSDELMERR
                     EEVEEKTVPAQCRFIVAAVDVQGGKNRRFVVQMVGYGENGERWLIDRYNISHTLPDSD
                     GVIEKIDPRIPDDWYILISDVLEKRYPLAHNPNHFMPILAMAVDSGGEDGVTDNAYQF
                     WRKCRRDGYAKRVYLVKGDSTKRQKLITKTYPDNTARSDRHSSARGDVPLYLLQTDYL
                     KDRINNALFRDTVGANYIHFPDWLGEWFFNELTYEERGADGKWRKPGKGNNEAFDLFC
                     YAHAIAILRGYERIKWGDENDVPSWAKLPDINPNIIRETPRTAEQAVEIEEKSEKPQP
                     KPTKAKSSWLSGGGRKTGGWL"
     gene            12999..13223
                     /locus_tag="PXD81_000347"
     CDS             12999..13223
                     /locus_tag="PXD81_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5536415.1"
                     /translation="MSLYTIDELKQKIRTLDEKIETAQSQVSFNGRSVSYQVAELTKQ
                     RDRYQTMLDEQLAQSGQRTKQHRIKYARFI"
     gene            13223..14722
                     /locus_tag="PXD81_000348"
     CDS             13223..14722
                     /locus_tag="PXD81_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDL5536416.1"
                     /translation="MNFLEKTIATLSPKWAANRSRNRYVLNAYEAAQPSRTHKASRES
                     KGANSTVRQSAVSLREQARALDQNHDIVIGILDKMEERVIGSKGIHIEPQPLTLAGEV
                     HEELAGQIRKLWAEWSVKPDVTGLYTRPLLERMLLRTWLRDGEVFVQLVKGKVAGLEH
                     GSQVAFSLEALEPDFVPMQSDEAKNGLVQGVFLNAWRKPTAYQVYLDNPQESNGMYGK
                     VKTVPADNMLHLAFRKRLHQIRGVSMLHGVIVRLADLKEYEESERVAARIAAAMTMYI
                     KKGDAALYDDDSNSDGERLFDIAPGAVIDDLKPGEDIGLINSNRPNVNLETFRNGQLR
                     ATAAGTRSSYSSIARDYNGTYSAQRQELVESFEGYAVLQDAFVAAISRPIYREWLKMA
                     IASQAIKLPPDIDQNSLFNAVYSGPVMPWIDPIKEANAWKERIKGGLATEGQAIRASG
                     NNPAEVKRQRIVEIKENQAEGLKFDTDLTNYGGQDKSFSNQKDEINEDE"
     gene            14734..16695
                     /locus_tag="PXD81_000349"
     CDS             14734..16695
                     /locus_tag="PXD81_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HDL5536417.1"
                     /translation="MKTWFNIQAKANDTAEISIFDEIGFWGVTAQQFAKDLKALGNNL
                     KQINLHIHSPGGDVFDGIAIYNLLKNHPANKTVYIDGLAASMASVIAMAGDEIIMPEN
                     AMMMIHKPWGIQGGDADDMRKYADLLDKVESTLIMAYVAKTGKYETDLAEMLKEETWL
                     TGKECVEQGFADKLAEPLVAMACIQSKKLEDYTKMPDKIKNMLFAPQGNAGANPKHEQ
                     PQTPPQAGENPQNVAKPDAMVALAQRNATIKATFAAFGTQFDGLLAECLADVSMTAEQ
                     AKDKLLAKLGENTTPSVPQNHIHAGNGNIVGDSVKQSLMARAGQDTDKTNARDNAYNA
                     MTLRELARASLVDRGVGIAGYTPMQMVGLAFTHSSSDFGQILIDVAHKSLLKGWETAT
                     ENYEQFTTRGTLTDFRPAKRVGLGEFGYLPEVGEGEEYTYGTIGDEGASVALATYGQL
                     FSITRQAIINDDMHLLTKIPEKMGAAAKATITKLVFALITGNATAQDGKKLFDATHKN
                     SLTGAALNVENIDKAIQLMNGFVNSRGEPLSIEPEFMLLPTSLHTKAKQILGSSSVEG
                     ADINSGIINPIRDIVSPIKSPRLQIADPKSWYLLNKEAIEVSYLDGVDSPYIEQQHGF
                     TVDGVSTKVRIDAGVNVIDYRGVVKVTNA"
     gene            16769..17092
                     /locus_tag="PXD81_000350"
     CDS             16769..17092
                     /locus_tag="PXD81_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2190 family protein"
                     /protein_id="HDL5536418.1"
                     /translation="MAKNYIQDGNTVRLTATKAITSGDVIVAEDLIAIAVSDAAKNDA
                     VVGLTTGVFSVKAKQADDIKQGAVLYWSETEGATLTAGSNKRLGIAWKDSGTSSNQVD
                     VKINV"
     gene            17085..17387
                     /locus_tag="PXD81_000351"
     CDS             17085..17387
                     /locus_tag="PXD81_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL5536419.1"
                     /translation="MSSPFEQAIAAADQTIQQTMMSEWLIGGKPYPATFDEAPAIFNG
                     LHSTDDRAIHGTERTLTLFRASGYKPRLDDRVSGNGKKYLVKSYHFVDQLIVLQLE"
     gene            17391..17915
                     /locus_tag="PXD81_000352"
     CDS             17391..17915
                     /locus_tag="PXD81_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL5536420.1"
                     /translation="MGSQVTGLKELQASLKKIARQTVPKAAAQAIRTVGRQAMNKAVK
                     SVAAEIGVNQKTIKGRAKMTAKPTPSRLQATIKVNRSHMPMIRVLERKSNRLSPTKGS
                     IRVGKHTVQRGFRQRLANGRTHIMYRQGRKRYGIDVAKVPLSQPLTQAFDRELKNYPE
                     QVQAELAKQIAGKL"
     gene            17912..18307
                     /locus_tag="PXD81_000353"
     CDS             17912..18307
                     /locus_tag="PXD81_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL5536421.1"
                     /translation="MKIHTKIRKEVLELLQTKLPEIDNFYNGLPTFIDIEEEQLAVSV
                     YLDDISLQEITVCNNQWDAKLNITIHLKSTESAEDELDEWAEKIREIIDDYPEFEHLE
                     SVALSQYQYEQDQNQRTWHMATLIFDVEY"
     gene            18335..18976
                     /locus_tag="PXD81_000354"
     CDS             18335..18976
                     /locus_tag="PXD81_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5536422.1"
                     /translation="MAKTTKVQGTKFRISVGREAQKAITAINLNTATLTIASSGYKKG
                     DAIELTGCGQLDGIYPVLSVTTDQVKLCEEVNWTGKDLPANYTKAKAALVQYSEQFCA
                     VKNIEKSDDTLSTEDVTTVCSEGTETEPGEIEFGSIKLSFFHKPTTEMQTRLRKLFYD
                     KSTFAYKLELPDNHGTTYGEGFIEAGNGFSGEVKGKYEGSVSIKPSKRDYLLV"
     gene            19060..19461
                     /locus_tag="PXD81_000355"
     CDS             19060..19461
                     /locus_tag="PXD81_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5536423.1"
                     /translation="MTLREKLLANKPKLQPIEINGETYYLREATVGDMNKQIFETRSW
                     LIQQAEQENVELPAEDDETFDEALNRFGEKYRLAQSVAYRLCDENGVLLFNPLDINDL
                     NAIAELDSKVIIDFNQAVSAPKDSASEESSK"
     gene            19506..19736
                     /locus_tag="PXD81_000356"
     CDS             19506..19736
                     /locus_tag="PXD81_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4035 domain-containing protein"
                     /protein_id="HDL5536424.1"
                     /translation="MPERHFAEYRLFYQEQPFGLWRDDYRTAQLAHLTAMFNRDPKGK
                     APKLAEFMPFFNRTDEVEEIEDDGVADYLAKR"
     gene            19799..20026
                     /locus_tag="PXD81_000357"
     CDS             19799..20026
                     /locus_tag="PXD81_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5536425.1"
                     /translation="MFEALDRFMINTIKIAIIVFLVACIFISMPNWWIASILVLFPTA
                     YLLDKYWWRNTEMYRRKMDKLVKEMKNNRQI"
     gene            20080..23631
                     /locus_tag="PXD81_000358"
     CDS             20080..23631
                     /locus_tag="PXD81_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HDL5536426.1"
                     /translation="MSSSNRLTITLDVENSKLLAGFAQSDTVANKFARSFIVNMDQAQ
                     QKARQFADRSTKYLKNIEQAANNLNSSVSKERFWQIANRLQFPTSKVISYANSYTELG
                     NKLKLVTESESQHARAMAAVYDISLKTAQSTQATSSVYQTFAQNAKQLGISQNDVAKL
                     TETVAKSVAISGASSATASNALVQFSQSLLMGKLKAQEFNSLMTQTPSVIQAIAKGLG
                     ITTAELKAMVDNGEMSAEKMIEGLKKAESYVNGQYAKTTTTISGAMQNLSTAAEKWVG
                     EMDSTVGASQKAVAVLNALNAHFDTFAKTALVAGGAFVLFKGYMSVRPFIEARAAIYE
                     NRKAIIAENQARQQTIATRQQELVVLSQQTALKLEQTLQTYNQNKAELEAIAVKQQKI
                     TKEREEIAVAIQAAATRTERLALSAELQALDQKEIALGQQKIAVERQQIATKQALKVA
                     YAENAVAQSAMNAGMVTATTTTRAMNGVLAAARAELNLLKAAALSNPLMTLAMVATTA
                     GTALWGFFESQKAAREEALRYADSLDSVRANIEKMTKAQTDAEMVKLSRSIKEQETAL
                     ASLKQRQAELTREIEQGATVLDDAFGAGVRFEKSAKELAKAQEELKLVTADVEKAQNT
                     LNASLEAQEALQAHVPIANLREEFVKLYPHIDESQIKVDGLNIAIGNFTVKSPEMVIA
                     ANNIANALGGVAGEAMRAAILVANLSSMKLDANGAAIIDPKHLEKIEQIERNNAIASA
                     KGKDKIALQVKDALIKSGMKEGDAGYERLKAAYEQQFTLQNAPKRGGKPKKTKAERSA
                     ESYQNQVAEMTNRLSGLKIDKEDLVKYGGISQYQEVRKLTEDIALNAEKYKNYGTEGV
                     AKLKELAAKIDSEQQLLDIEQFQVDHDKKLEAMQFELDLLGKTRTEKELLQFNNQLDL
                     EAERLKINMTKENIALLDQKIAKIKEEYELHQKKQEETRASVMMGIKEGWNNIETDVS
                     NVAANVANITQNTFDGMADSLTNFVMTGKADFRSFANSILSDLSKMIIKMMLFNAIKQ
                     GATMLFGYQGPTTTVNGNAVYGLGTWATGGYTGNGGKYTPAGIVHKGEYVITKEATSR
                     LGLDYLNYLNYGRRGFASGGGVAVPRVPSSSYQPKSAQSSISVQVINNGEPVDAKVSQ
                     KQQGEQTQITVELMRKIARQEANGMIQNNFRAGGVFA"
     gene            23631..23960
                     /locus_tag="PXD81_000359"
     CDS             23631..23960
                     /locus_tag="PXD81_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL5536427.1"
                     /translation="METLQWCVRTDLAVENTPEIMEVKFGDGYTQRSPKGLNNLLRAY
                     TGTIKVKKGEHLAVEAFFAKHRGVSPFYFKDPYTQQNKKVVCSSWPAKMVGLNHWEFS
                     VTFKEVP"
     gene            23960..>24543
                     /locus_tag="PXD81_000360"
     CDS             23960..>24543
                     /locus_tag="PXD81_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HDL5536428.1"
                     /translation="MPVSISNQMKLDLAKLEQNAMLDLYEVDLRTLKDKSGNAGSVYR
                     FYSGLNELKTSIVWQGRTYDPYPIEASGFERNSSGPSNRPTLTLSNLFGLVTGIANQF
                     DECIGAIVRRHQVYAQYLDAVNFAGGNAKADPNQEIISHFVIEQLTSLTRETATFTLA
                     LPIETDNAKIPSRIIMADTCTWIYRSAECGYTGK"
BASE COUNT         7582 a         4885 c         5564 g         6512 t
ORIGIN      
        1 tttctttgtt ggtctgaacc acttaatcca ataatagctt cacaaagatt atgacttact
       61 ccttcaggta tcaattcagc aaaaatagtc ggattgtgat cataatgagt aattttttct
      121 ttcagcttgt tcaggctgtt aaccaaatta tgcgatacac tccgagaaaa tgaaattgcc
      181 tgaataattt cattctgttc cttaatttca atattctcaa caggataaga taaattgaca
      241 atatagcttc caatttgagt ttgccctaaa cgaacttgag acataaactc tgctacatct
      301 ttgccacctg agttatgtcc aaaagctgcc tttttctttt tagctgaaag tgccaaggca
      361 ttaattaaat gtttggtttt ttcaaataat tttacacctt cgtttagcgg aattgtccca
      421 tctttaacgc tttcgccaat cactctaaca gatagcttgt ctgtatattg atttgaaata
      481 ttaagcttta acgtttctac gtttttttct tcaattttgg cgagcttttc aagtgctttc
      541 tgcaacactt cctctctatc ttcagcaatt ggctttggta atgtaacctc taaaattcga
      601 tgtccaagat ctttctgcca aataaccgca tactctaatt caaacgcaat ttgccagctt
      661 ttggcagtta aatagcgagc aaactgttcg agaaacagta acgtgtccgt tttattgttt
      721 ttcataaagt ttctcctact ctagcgtatt caatcatttt aatgagtaca tcagtggtaa
      781 atcgttgatt ttctggaaat gtgagggtaa cactacttct atttgttgtt gccggaaagc
      841 cttttaatga tacccaataa caatgtcttt ttaaagtcat aaattttttc ttatgtgaga
      901 gccattgagt cgttttttta ggcaagtcac atacaattaa gtagcgaggg ttcgcacaat
      961 ttttccttaa atcatcatag tttttctttt tgagtttata gcttaccaga ccatttttaa
     1021 tatgaagatc tccttctgat ttaagttgta gctttatttc cggatcactt cttttccctt
     1081 cttcagtcgg aaattctgca gaaattgcaa gatcaacact atgattgtct actactggta
     1141 ccgcatgatt aaagcctgca ttagcagtaa tagctaacac ataagcaata ttaaattgtt
     1201 ccatttgttg tgtaggtgaa agcatatttc tatccaataa aattcaactg ttggctttgg
     1261 tgaactttta ctctagcatg gataaatagc tgatcttgat tttcttcagt gatactccac
     1321 ttttcataga gtttgttatc tgaaatcact aatatttgat cgcctgtatt ttgtaatctt
     1381 tttacataca aatgtccttt gtatgtaaat acataaacac catcgccggt aaactcattt
     1441 acgctaatat caacaaaaag catatctccc gaggaaaatg tcgggtacat agaatcacca
     1501 cttaaattga taattctgat gtatcgaggt gccatattcc gaaagaggga ataaaattgc
     1561 tcaggcacat aacgcatttg atttactact tcaactaatt cactgcttga gccgaagcct
     1621 gcactagcac tgacatctaa tacatcaatt atgataaagt catttaaatc ttgctcctgc
     1681 aatttttgtt cttcattgcc tatccttgaa ttgataaggt ttgattgctc tgaatgtatt
     1741 tgatctaacc agcctctagg tttattcatc ccatcttcta atttggcggc aatcttgtca
     1801 ccaatgcctt ttggcttacc gttctgaata gcatttttat ttttgatttg tagtagatag
     1861 cttactgcga tacctgttcg attagctaaa tcagatgtgc ttttagcctc atctatcaag
     1921 cgtaataaat tatcgtatct aatttctttt aggggtctca tatcccacct ccatactatt
     1981 tgctttattt tattatcatt tcgataacat ttaaatatga tctattttgt tgattttgat
     2041 ttatctttaa gataatattc acttatcttt taggtaagga ttatgaaatg caattacaag
     2101 aatacataga aaaatctaag tggggggaaa tctctcgatt agctaaagcc atcgatgtag
     2161 caattccaat agtttcattt tgggtaaacg gcaaacgcca agtaccggca aagcgttgcc
     2221 cggaaattga aaaatttaca gaagggaaag taacttgtga agagctacgc cctgatgttg
     2281 attgggcagt tttacgaaat tcaggcaaat aaaaaaccac cgctggaacg gtggctaatt
     2341 acaacctaag gaggtttatt ttgaaatccc aatcattatt accgataacc gcacaaaatg
     2401 caagcggtgc ggaagtcaaa atcagcagta ttgaaattgc agagttatgc gaaaaacaac
     2461 ataagaacgt tattgtagat attcgcaaga tgttagctga gctgaaattg aactcggctg
     2521 atttttcagc ccagtatcaa accaaagacg gcagaatgca accctgctac tttcttccaa
     2581 aacgagaaac aatgatttta gtttccggct atcgcattga tctgagagcc aaaatcattg
     2641 accgcttaga tgagttagaa aagcaacatc agccctctat tccgaaaaat taccccgagg
     2701 ctctgcgaat ggcagcggag ttggcggaac aaaaccaagt tttattgttg gaaaaacagc
     2761 agactaccgc agaaaaccac gccttgaaat cctactttga accgggtttg acccctgctc
     2821 agtttgtaaa agggctgaat ggcgtaaata gtaacaagat aaatgattat ttacgtagtc
     2881 gaggctggtt atacaaagac aagcattgct ggcgggtgtt tagccacgtt cgggataaat
     2941 atctgacgga gtcatttaag agaattgaag ttgatcctgt tgaacgtatt gagatggcca
     3001 cctacaagcc aattttgctt gagaaaggtg ctgttaagat ttttgaggct tatttacaag
     3061 gtaagttacc aatgaaagcc gattggaacg gcaagtatta tcacagtaag gtggcggtat
     3121 gagtatgtta ttaatggtta aggcgatgaa ttgtacggtg ggaaattctg cccgtaaatt
     3181 agtgctgtta aaactcgctg ataacgcaaa tgatgatggt gtttgctttc ctagttatca
     3241 atttattgca gataagtgtg aaatgtctaa acgcagtgct attagccata ttgatgaatt
     3301 aatcaaaatg ggatatgtga gcaaaaaatc acgcaaaaac aaagatgggt ctagttcaaa
     3361 tttatatcaa ttacaccttg atgcccctag tgaaaaatct gcactagggg gtgaaaattt
     3421 tgcaccaggg ggtagtgaaa attctgcacc cataaccagt cactctatta accagtcaat
     3481 taaccataat acgtccgaac aaaattcgga cggggtggat aaaaaatcaa aatatcgttt
     3541 ttctgataac gatatggcag tggcaaagtg gatttttaaa cgagtgaaac aactagctcc
     3601 tcagaccaag gagtcttact tgccgaattg ggccaatgaa gtccgcttga tggtagaacg
     3661 tgataaacat actcaccaag aaatttgctc gttgtttaaa tttgcgaccg aacaccattt
     3721 ttggggcagg aatatcctaa gtgttgtatc tcttcgagct aagtgggatc ggctaaatct
     3781 agaaaggcaa tcatcattac gaccaaacaa tattcactca gcaacaggca atggtgatat
     3841 tcaagacaca acggggtttt caaaaggtct tgttgttgat gtaggaggag ggacaaaatg
     3901 ggaattttag ctacaacgac tcaaaatcaa gtattacctg ttgagattgt ggaacaagca
     3961 gaggtggaaa caaccaagat tttcaaccgc ttgaaagcaa ctcacttagg ctggcgagcc
     4021 aatatcaaga gtcaagccga atatgataat gctcgccgtg aatgggttcg tttgttgatt
     4081 gaaaatcgct taacgccaaa agaagttgaa cgaggtttga aaattgcgga taccgataaa
     4141 aatccctatt taccgagctt tggacaattc ctcgaatggt gtaaatccat tgactacgca
     4201 gaattaggtt tgcctgatat ggatcaattc ctcaagcgtt taaatcattt ttccgctttt
     4261 gggtttactg aaatcgataa ttttaagttt cgttcaaacg ctgaatattg gctcttaact
     4321 gacctttatg aacgaaatcg aaaacatagt tggaaagatg aaacattacg taaccaagct
     4381 gaaaaagcgt tagtagagat ggcaaaacgg attatgagcg gtgaacaaat tccacctcct
     4441 aagccagtaa tcgaagagaa aaagaaagtg ccaatagatc cacgcattca gcgaattttg
     4501 gacgagaaga aacaagcagg agcaatgcaa tgacagattt tgataaagac acttaccaaa
     4561 cacctaatta tgtgcgtaat tggctaaatc atcgctatgc gtggtttcac attgacggtt
     4621 gttctaacgg caaaaacgca ttgtatagcc actggattgg caaagcggca gagggcttgg
     4681 acgaagacac gctaagtggt cagattgccg aggatttctt ggcagaaaac ttgctcgatg
     4741 tgatcttgga acgtgtggca gattggggcg aattactccg tatcttcgtt aatccgccat
     4801 atagcaatcc actccctttt gttcagcgtg ctgcggagct gaaaaaagcc ggtcatattg
     4861 tggtaatgct gttgccggca gacaaatcta ctcgttggta tcaggttatc caagaaaacg
     4921 ctagtgaggt gatcgacatt atcggtgggc gtattaattt tattaatccg ctcacaggcg
     4981 aggaagtgaa aggcaacaac aaaggctcga tggtagcggt gttcgacccg tttgtggagg
     5041 gaatagttac ccgtcaaatg ccgctggata agattaagga gctgggcggc tatgaaaaat
     5101 aacgaagggt gggaagtgtg atgagtgatt ctactattgg atttttaggg attctaattt
     5161 tcttcgggtg gttagcatat ttggtattta gagcttttgg attttcattg agggatagag
     5221 atggctaaat accaaatgat gaaattgccg ggtgggattt taagcccgtt aaacgatgaa
     5281 gaagccgagc ggttgaaatc gtttcagaat ggcgagcagt acgaaatcga gattaaacgc
     5341 cagcgtaatc ccgcttttca tcgcaaggtg tttgcgttct ttaatttctg ttttgattgc
     5401 tggagtggta gccattccga ttgggagttc caagatccaa ccgctcaatt tgatactttc
     5461 cgcaagcatt taactgtaat ggcagggttt tatgtgcaga cgtggaattt aaaagggcaa
     5521 gtgcggattg aggcgaaaag cctaagctac gccaatatgg aacaagacga gttcgagcag
     5581 gcttataaag cgttgattaa tgcagctatc aagcacgttt ttaacaatac caaagatgag
     5641 aatacgataa atcagttgta ttcgtttttt taattaaggc gaaatcgcca tatttaatta
     5701 ggtttaattg caggagatta aaatgaaaaa gaatgttatt agatattggc ttagcgtgcc
     5761 attcagaatt attgcagtgc ttatttcttt gtcaattctt actcttagat atatcgggta
     5821 cttattgatt tatccattag cattttttgt ggcagtcggt agttttattg ttatcggtca
     5881 atggaaatat caagaatgcc ttgatgactt taagagcgat tttaatccga tttagtgtgt
     5941 gtttattaat tccctcgaat tcgggggtag gagaaatcaa aatgacaaaa attctttgaa
     6001 actatcgcac tcgtgatgtt atttggcatt attccaagct gtttattggc gattaatact
     6061 actttaatga atattaaaaa cgcaccgtgg tttatggtga ttattcagtt agggttttgg
     6121 attttaatat cgtttgttat cggttgcgga ttattccttt tatgtggaat gttatttaat
     6181 ttaaggagat taataagtga gccagtgcaa tgaactgact gtaattttaa tcactatcgc
     6241 tattcttagt gtatgtgccg gttcactctt ttttattgag tggtttgata aaagagacca
     6301 aggaggttgg aatggctaat ctacgcaaag aggcaaaagg cagagagtgc caagtccgtt
     6361 taccgggtat ctgtaatcat aatccggaaa cggtggtatt ggctcactat cgaatggcag
     6421 gattaaacgg tgtgggaatg aaacccgatg atattttcgg ggcgtgggct tgcagtagct
     6481 gccacgatga gtgcgaccgt agaactcgca aattagaagc ggattatgtg agacaggctc
     6541 atgcagaggg cgtatttaga acgcaggcta ttttacggag agaagggaaa ttatgagtga
     6601 ttggcttgaa attgcgttgc cttatccgcc gagtgtgaac cattactgga agcatacccg
     6661 aaacggtagg cattatattt cagataaggg gaaagccttt agggcggaag tggtgaaaat
     6721 ctgcaaaatg tttgatccgt ttaaaggagc ggtacaaacg gtattgcaga tttactatcc
     6781 tgataaacgc aagcgtgatc cagataattt agaaaaagcc ttgtgggacg cattaaaaat
     6841 cggtggctta attgaagaag acgataatca aattttagtc gataagcgaa ccattacggc
     6901 aggttttaag aaagggggaa tggtggtgtt aaagattaag gcggtgggta atgcaaattg
     6961 atgtaaaggg tgtattaaat ttatggggtg gatatgtgcg ttttaccgac tgcaagggct
     7021 atccgacaat gcaggcattt atgcgtgagt ctccacaaga gagaacaaaa cagcgttatt
     7081 tagcccgctt agatgatgat acgcttgaga aagtcgatcg gcaaatgcgg aagctacaag
     7141 cgaacgacaa gcgacaattc gatgtactat tttataaata tgcagtaggg ttagagaata
     7201 agcaggtttg gcagaagtta tcgctcagca agacttcata tcatacagaa ttacttcttg
     7261 ctgaacgatt tatggagggg gcattagccg gtagtgaaat tcgtttgctc ttgtagcttc
     7321 tcaacggcta atttaattag ttgattttgg ggaatattta actgttgggc aagttgctcg
     7381 atttgtgcga ttgtgctttc gtgcagttta aaggctttaa gtttaattcc ccgttttgcg
     7441 ttgctacggt ttacaatttc ggttcttgac agtgccatca taaatcctta atataggttt
     7501 tgaagagtag ggagatttct ccccctactt agtttcattc aattagtatg ctggcaggct
     7561 aatcactaag agaatgataa ctaggataat gcggtaaagc atttgttatc cctccaaaag
     7621 tcggtgggat taaaggtcgc caccggctcg tttccagcac tattactgaa aacaagttta
     7681 ttatatgttg gataaccaat atattcaagc aaaatatcgt taaaaagccc atggttttgt
     7741 aaattatggg cttttttgca tttaaatgat taaaaagtgt tgcaaagttc ggacttaatc
     7801 tatattattt atggtaagtt gcggttcatt gcataggtgg tgaacttaaa agaattttac
     7861 agccctgagc agaaatgctt ggggcttttt tgtttacgga ttttaagggt gtagctcaat
     7921 aggtagagca acggtctcca aaatcgttgg ttgttggttt gagtccaacc accctttcca
     7981 aatgcgagta aatgttatgc tggaacaact aaatactcag tcaaaaagcg gtgtagtaaa
     8041 tccaaaaaga tttctaaatc ctgatgtcga ggactaccag cctgaaagta tatcaagatg
     8101 cgctcttcgc ttccgaagta agaaaatgga cgaatactcc agaaaagagc ttgtgaaact
     8161 atatcaaatg cttgctcagg cacggaaaat tcaatagata actgcttttt attttcttgc
     8221 aagaattgct cgaactgttc atcaatactt ctgataaatt ctaggttagt tgcaactgaa
     8281 tgttcccgga atgaaaactc taagtaagcg tatgatgtat ctctattgtc tttatgagcc
     8341 caataagcac agcctagtgt tagaagtttg ctgtttggag tgttcaagtc tgtgagtaac
     8401 ttttttaaca ttggggagtg tgcggcttct ggtattagtt ctaccttttc aggattggaa
     8461 acaagatcta ctccaccaat acaaggtgtg gcagtgtcgc ttgaagtgtc gcccggacgt
     8521 ggtctaaatg ggaagtgata gaaactatcg tcaatttgat caaattcaat catttttaac
     8581 ctctgctagt ttatttgttg gggaacagta ttctagcaga atttttaacc aaggctcagt
     8641 tttacgactg ggctttttta ttgcctcgaa acggggtgga gtatgaatac aatgcctgaa
     8701 aagaaccctg atatatatac gcaggcttgg aattatcttg taatgttttt aagcaatcat
     8761 aatggctgga tttgtggcgt tatcattgct ttttttacat ctattaccaa gtcatttctg
     8821 tatggtaaaa cagatacacc taaacgagtg ctggcagagg ctatcttgtg tagcgttatt
     8881 gctggctcaa tgcgtcctat tttaatttat ctcaatgctg atgtatcact gattacccct
     8941 attggtgcgg gaattggatt attgggtaca agtgcaattc gccagatgat tttaaaattc
     9001 ttgaacaaaa aagcaggtgg tgaaaatgtg gattagtgaa agtaagttta aacaagtatt
     9061 ccctaatgca gttagtggta tttactcaat tattgacaaa cacattgaaa aagcaggctg
     9121 caaaacaaaa gagcaacaag cgatgttctt ggctcagtgt ggtgtagaat gtgccggata
     9181 tagtgtattt gaagaaaacc ttaactattc tgcaactgct ctattgcgaa cattcccgaa
     9241 atactttagc caacacaatg tcgtacttta tgctcgagat aagcgtgcta ttgctaatcg
     9301 ggtgtatgca aatcgaatgg gtaacggcaa tgaacaaagt ggcgatggtt ggaaatatcg
     9361 tgggcgtggc ttaattcaaa ttaccggtaa ggcaaattat atcgagtttg ctaaatggga
     9421 aggcttacca agtctagtga ctgaccccga ccaaatcact aaaaatctag cattgacagt
     9481 acaggtggct gtttggtact gggtaagtcg caagttatcc gaatttagcg acattcttac
     9541 tgttactaaa aagattaatg gcggaacgca cggtctaact cagcgtacag cttattataa
     9601 aaagctaatg gcggactaaa tgattaaata catttatcta gctgccacag taatgatttt
     9661 gggtttgtgt ggctggattt ggcaccagtc caagcagatt gatggcttaa gagccgaaaa
     9721 ccaaacacaa gcccaaacca ttgagcagca gcaaaaagcc aatcaacggc taaccgatag
     9781 cttagagcaa gagcgacaag cggtcgaaaa aatccaaaaa attgcaaatg agctgcggaa
     9841 taaagtggag atggccaaga atgaaattac atcaatactg gcacaagaca gttgtgctaa
     9901 ggctgatttg cctaatggtg ttgctgatag cattaagcgg ttgcactagc aaaacaatcg
     9961 tcagcactga gtatttatat ccgcctgcgg cttaccttgt gccgtgcgag cggacagcat
    10021 ttagcggtaa aacctacggc gatacggtgg attatctcat taaagtaatg ggagagcgtg
    10081 atttgtgtgc gagtcaaatt gaccgtatta gagagtggca agcacagact aagcaagggt
    10141 ttaaataact tggcggatta aaccgctaat gagagtggga gcttaattgc tcccttttca
    10201 tttgcgttac attacttagt tatgcttttg attgtatcaa gatcataact aagcaatgtg
    10261 ttgcatagtt tgtaaaaggt actcccgagg ggatacccct ttccacgggg ttgcggcagc
    10321 gtggttttcg gcaatttttt gagtttctag gcatcatcat cttcctatga tgttgctgtt
    10381 ttttaggcgg ttttgatttt taggatttga gcaaatggac aacttatttg atctaaaact
    10441 taacatcaat cagatcgccg aactcacagg attgcaccgc cagacggtat ctcagcgagt
    10501 cgctgggtta aatccagcac tcggtagtaa tagcaaatta aaactctatg ctctgcgtga
    10561 tttaatcctc accgggttgg cggaaaagat gtcggcagat gtcgatagcc tatcacctgc
    10621 tgatcgtcgt gcgtggttcc agtcggaaaa cgaaagacta aaatttgaaa aagaaattgg
    10681 cgagcttatc ccagcaagcg aagtggctct tgaaatgtca gctttagcaa aaacggtagt
    10741 acaagcctta gaaaccttgc ctgatatttt agagcgagac tgcggattgc agcctaaaga
    10801 tttgatacga gtgcagcaag taactgacga tgtacgagac caaatggcat tacatatcca
    10861 agaagttacc actgacacgg agaatgaata atgtttgcta gtgctaaaga aattcgacgt
    10921 gatatagcaa atgccataaa agcacctcgg cgaatgagag tgtcagaagc agtagcggaa
    10981 tatatgcgtg taccacttgg cggaggcaac tcggtcaaat gggataagca caccgccgcc
    11041 tatatgttag aaccgatgga ctgcctcaat agccgagaat acgatgcggt gatttttgtc
    11101 ggcccggcac gaacgggtaa aaccatcggc ttgattgacg gctggataac ctactcaatt
    11161 atctgcgatc cgtctgattt cctgttggtg caacttaccc aagagaaagc tagcgaacat
    11221 agccgaaaac ggcttgaccg cacattccgc tgttcgcccg aaattgccaa gcggctaagc
    11281 ccacgcaaaa acgataacaa tgtccacgac aagtatttca gagcgggcaa cttgctcaaa
    11341 atcggctggc cgtctatcaa tgtgctttct tcatcggact acaaatatgt tgctctgact
    11401 gactacgacc gctggccgga agatgtggac ggagagggcg acggtttctc gcttgcctca
    11461 aagcggacga ccacctttat gtcagcagga atgactttgg tggagagttc gcccggtaaa
    11521 gatattgttg atctcaaaca tatcccgaaa agcacccacg aggctccacc gactaccggt
    11581 attttaagcc tctataatcg aggtgatcgc cgtcggtttt actggcaatg ccccgactgc
    11641 tccgaatact tcgagccgtc aatggcaaat atggtcggct accgtgaaga cagtgatttt
    11701 gtcaaagcca gcgaaaacgc acggttacaa tgccctcact gccaaaccct gattgccccc
    11761 gaactcaaac gagagctaaa tatcaagggc gtatggctca aagaggggca aaaaattgat
    11821 gcaagcggtc aaattagtgg cgaaagtcgc aaatcccgta ttgcctcctt ttggcttgaa
    11881 gggccagcgg cagcctacca aacgtgggcg cagctcacct acaaactgct caatgccgaa
    11941 cacgaatatg aaatgactgg cagtgaggaa accctcaaag cggtaacaaa taccgactgg
    12001 gggttacctt acttaccacg atctgcatta gaacaacgcc gttctgatga gttgatggaa
    12061 cgccgagaag aagtagaaga gaaaactgta ccagctcaat gtcgctttat tgtggcggcg
    12121 gtggacgtac agggcggtaa aaaccgccga tttgtggtgc aaatggtcgg ttacggtgaa
    12181 aatggcgaac gctggttgat tgaccgctat aacatctcac atactttgcc cgacagtgat
    12241 ggcgtgattg aaaaaattga ccctcgtatt cccgatgatt ggtacatact gatttcggac
    12301 gtactagaaa aacgctaccc acttgcccac aatccaaacc actttatgcc gattttggcg
    12361 atggcggtgg acagtggcgg tgaagatggg gtaaccgata acgcctacca attttggcgc
    12421 aaatgccgcc gagatggcta tgcgaaacga gtctatttag tcaaaggcga cagcaccaaa
    12481 cggcaaaagc tgattaccaa aacttacccc gacaataccg cacgaagtga ccgccactcc
    12541 tccgcacggg gcgatgtgcc gttgtacttg ctccaaaccg actacctcaa agatcggatt
    12601 aacaacgccc tgttccgtga tacggtgggg gcgaactaca tccacttccc cgactggcta
    12661 ggtgaatggt tttttaacga attgacctac gaagaacgag gagcggacgg caaatggcgg
    12721 aaaccgggca aaggcaataa cgaagccttc gacctgttct gctatgccca cgccattgca
    12781 attttacgag gctacgaacg aatcaaatgg ggcgatgaaa acgatgtgcc aagctgggca
    12841 aaactgcccg acatcaaccc taatatcatt cgggaaacgc caagaacggc tgaacaagcg
    12901 gtcgaaattg aagaaaaatc cgaaaaacca caacctaaac ccaccaaagc aaaaagcagt
    12961 tggttaagtg gtggcggtag aaaaacagga ggctggctat gagcctttac accatagacg
    13021 agcttaaaca aaaaatccgc acgcttgatg aaaaaatcga aaccgcccaa agccaagtga
    13081 gctttaatgg tcggtcggtt tcatatcaag tggcggaact aaccaaacag cgtgatcgct
    13141 atcaaacaat gctggatgaa caacttgccc aaagcgggca acgcaccaaa cagcatcgaa
    13201 ttaaatatgc gaggtttatt tgatgaactt cctcgaaaaa accattgcca ccctttcccc
    13261 gaaatgggcg gcaaatcgct ctcgaaatcg ctatgtgcta aatgcctatg aggcggcaca
    13321 gcctagccga acccataagg caagccgaga gagcaaaggg gcaaactcta ctgttcgcca
    13381 aagtgcggtc agcctacggg aacaagcccg agccttagac caaaatcacg atattgtgat
    13441 cggcattctc gacaaaatgg aagagcgggt gattggctca aaaggcattc atattgaacc
    13501 gcagccgctg acgttggcag gtgaagtcca cgaagaatta gctgggcaaa tccgaaagct
    13561 gtgggcggaa tggtcggtga aacctgatgt gacgggatta tacactcgcc cattgctaga
    13621 acgaatgcta cttcggactt ggctacgaga cggcgaagtc tttgttcagt tggtgaaagg
    13681 caaggtggca gggttagaac acggctcaca ggttgctttt tcccttgaag ccttagagcc
    13741 ggattttgtg ccaatgcaat ccgatgaggc aaaaaacggc ttggtgcaag gcgtgtttct
    13801 caatgcgtgg cggaagccga ctgcttacca agtctattta gacaatccgc aagaaagcaa
    13861 cggaatgtac ggcaaagtga aaaccgtgcc ggcagacaat atgttgcacc tcgcctttcg
    13921 caagcggtta catcaaattc gtggtgtaag tatgttgcac ggtgtgattg tgcgattggc
    13981 ggatctgaaa gaatacgaag agagcgaacg agttgccgct cgcattgccg ccgcaatgac
    14041 gatgtacatt aaaaaaggcg atgcggcact gtatgatgac gacagtaaca gcgatggcga
    14101 acgcctgttt gatattgccc ccggtgcggt gattgatgat ttaaaaccgg gtgaagacat
    14161 cggcttaatc aattccaacc gcccgaatgt gaatttggaa actttccgca atggacaact
    14221 gcgagccact gccgccggta ctcgttcgag ctattccagc atcgcccgag actacaacgg
    14281 cacttactct gcacagcgac aagagctggt tgaaagtttt gaaggttatg cggtgctaca
    14341 agatgcgttt gtggcggcaa tcagccgccc gatttatcgg gaatggctca aaatggcaat
    14401 cgcctcacaa gcgatcaaac tgccgcctga tattgaccaa aactctctct ttaatgcggt
    14461 ttattcaggt cctgtaatgc cgtggattga cccaatcaaa gaggcgaatg cgtggaaaga
    14521 acgaattaaa ggcggtttag ccaccgaagg gcaagcaatc cgagccagtg gcaataaccc
    14581 agccgaagtc aaacggcaac gaattgtcga gatcaaagaa aatcaagccg aagggctaaa
    14641 atttgatacc gatctcacca attacggtgg gcaggataaa tctttctcca atcaaaaaga
    14701 tgaaattaac gaagatgaat aacaggaggg tgcgtgaaaa cgtggttcaa cattcaagcc
    14761 aaagcgaatg acaccgccga aatctcaatt tttgacgaaa tcggcttttg gggtgtcacc
    14821 gcccaacaat ttgctaaaga cttaaaagcc cttggcaaca atttaaaaca aatcaatctg
    14881 catatccact cgcccggtgg tgatgtgttt gacggcattg ccatttacaa cctgctaaaa
    14941 aatcaccctg ctaacaagac cgtctatatt gacggtcttg ccgcatcaat ggctagcgta
    15001 attgcaatgg caggtgatga aatcattatg cctgaaaacg ctatgatgat gatccacaaa
    15061 ccgtggggca ttcaaggcgg tgatgcggat gatatgcgta aatatgcgga tttactggat
    15121 aaggttgaaa gtacactgat tatggcgtat gtcgccaaaa caggtaaata cgaaaccgat
    15181 ttggcagaaa tgctgaaaga agaaacttgg ctcacaggca aagagtgcgt ggagcaaggt
    15241 ttcgccgata agttagccga accccttgtg gcgatggctt gtatccaatc caaaaaatta
    15301 gaggactaca caaaaatgcc tgacaaaatc aaaaatatgc tattcgcacc acaaggcaac
    15361 gcaggtgcaa atccaaaaca tgaacaacct caaacaccac cacaagcggg tgaaaatccg
    15421 caaaatgtgg caaaacctga tgcaatggtg gcattagccc aacgcaatgc aacgattaaa
    15481 gcgacctttg ccgctttcgg cacacaattt gacggcttgc tggcagaatg tttagctgat
    15541 gtgtcaatga ccgctgagca agccaaagac aagctccttg ccaaactggg cgaaaacacc
    15601 accccaagcg taccgcaaaa ccacattcac gcaggtaacg gtaacatcgt tggcgacagc
    15661 gtgaagcagt cgttaatggc tcgtgctggt caagataccg acaaaaccaa cgcaagagat
    15721 aatgcctaca atgcaatgac cttgcgtgag ttggctcgtg cctcattggt tgatcgtggc
    15781 gtgggtattg cgggctacac cccaatgcag atggtcggtc ttgcctttac tcattcgagc
    15841 tccgacttcg gtcaaatctt gattgatgtg gctcataaat ccctactcaa aggctgggaa
    15901 accgctaccg aaaactacga gcagtttacc acccgtggca ctcttactga cttccgaccg
    15961 gcaaaacgag tcggtttagg tgagtttggc tacctgccgg aagtgggcga aggtgaagaa
    16021 tacacctacg gcacaatcgg tgatgaaggt gcatctgttg cattggcgac ttacggacaa
    16081 ctgtttagca ttacccgtca ggcgattatc aatgacgata tgcatctttt aactaaaatc
    16141 cctgagaaaa tgggtgcagc agccaaagcg actattacca aactggtgtt tgccttgatt
    16201 accggcaatg cgacagccca agacggtaaa aagctgtttg atgctacaca taaaaacagc
    16261 ttaacgggtg cggcattgaa tgtggaaaac attgacaagg caatccagtt aatgaatggc
    16321 tttgtaaaca gccgtggtga accgctctct attgagccag aatttatgct gttaccaacc
    16381 tcattgcata ccaaagcaaa acagattctt ggttcgtcaa gcgtggaagg tgcagacatc
    16441 aacagcggta tcattaaccc gatccgtgat attgtcagcc cgattaaatc gcctcgctta
    16501 caaattgcag atccgaaatc atggtacttg ctcaataaag aggcgattga agtctcttat
    16561 ctggacgggg tggatagccc ttacatcgag caacagcacg gctttacggt ggacggtgtt
    16621 tcgaccaaag tccgtatcga tgctggtgtg aatgtgattg actaccgtgg tgtggtgaaa
    16681 gtgaccaacg cttaatttaa acaatttaaa acctgaccgc acttttaaca gtgcggtttt
    16741 ttattatccg aaaaaaggaa aaagagatat ggctaaaaac tatattcaag acggcaatac
    16801 cgtgcgttta actgcaacca aagcgattac ttccggcgat gtcattgtgg cggaggattt
    16861 aattgcgatt gcggtttctg atgcggcaaa aaatgatgca gtagttggct taactaccgg
    16921 tgtttttagc gtaaaagcta aacaagcaga tgacatcaaa caaggtgcag tgttgtactg
    16981 gtctgaaaca gagggcgcaa ctctcaccgc aggctcaaat aaacgacttg gcattgcgtg
    17041 gaaagactct ggcacatcat ctaatcaagt tgatgtgaaa atcaatgtct agcccgtttg
    17101 agcaggcaat agctgccgcc gatcaaacta ttcagcaaac tatgatgagt gagtggttga
    17161 ttggcggcaa gccttaccct gcaacttttg atgaagcccc cgctattttt aacggtttac
    17221 actcaaccga tgacagagct attcacggca cggaacgcac actgacccta tttcgggcga
    17281 gtggctacaa accgagattg gacgaccgag tgagcggaaa tggcaagaaa taccttgtta
    17341 aatcctatca ctttgtcgat cagttaatcg tattgcaatt ggagtaaaca atgggttctc
    17401 aagtgaccgg tttaaaagaa ctgcaagcct ccctgaaaaa aatagctagg caaaccgtac
    17461 ctaaagcagc cgctcaagca atccgtactg ttgggcggca agcaatgaat aaggcggtta
    17521 aatcggtggc ggcagagatt ggcgtaaatc aaaagacaat caaaggtaga gcaaaaatga
    17581 cggcaaagcc cacgccgtca agattgcaag ccacgattaa ggttaatcga tcacatatgc
    17641 cgatgattcg ggtactagag cgcaagtcca accgcctgtc acccaccaaa ggcagtatta
    17701 gagttgggaa acacactgta caacgtggtt ttcgccaacg actcgccaat gggcgaacgc
    17761 acattatgta ccggcaaggt cgtaagcgtt acggcattga tgtagcaaaa gtgccgttat
    17821 cccaaccact tacacaagca tttgatcgag aattgaaaaa ctatcccgaa caagtgcaag
    17881 cggaattagc taaacaaatt gcggggaaat tatgaaaatc cacacaaaaa tccgaaaaga
    17941 agtccttgaa ttattacaga ctaaattgcc ggaaattgat aatttttata acgggctgcc
    18001 aacttttatt gatattgagg aggaacaact tgcagtatcg gtttatctcg atgatatcag
    18061 tctgcaagaa ataactgttt gtaataatca atgggacgcc aagctcaata ttacaatcca
    18121 tctgaaatct accgagagtg cagaagatga attagacgaa tgggcggaaa aaattcgtga
    18181 aattatcgat gattatcccg aatttgagca tttagagtca gtcgcgctct ctcagtatca
    18241 atatgagcaa gaccagaatc agcgtacgtg gcacatggcg acattaatct ttgatgttga
    18301 gtattaaaca aataatctct ataggagact taaaatggca aaaacaacaa aagtccaagg
    18361 cacgaaattt agaattagcg ttggacgtga ggctcaaaaa gcaattaccg caattaactt
    18421 aaatactgct actctaacga ttgcatcctc aggctataaa aaaggtgatg caatcgagct
    18481 tacaggttgt ggtcaattag atggtatcta cccggtgtta tcagtaacta ccgatcaggt
    18541 aaaattatgc gaagaagtga actggacagg taaagattta ccagctaatt acaccaaagc
    18601 caaggcggca ctagtgcaat actcggagca attctgcgcg gtaaaaaaca ttgaaaaatc
    18661 tgatgatact ttgagtaccg aagatgtaac aacggtttgt tcagagggca cagaaaccga
    18721 gccgggtgaa attgaatttg gttcgatcaa actcagtttc ttccacaagc ctactaccga
    18781 aatgcaaacc cgattgcgca aactctttta cgacaaatcc acctttgcct acaaactgga
    18841 actaccggat aaccatggca cgacctatgg cgaaggcttt attgaggccg gcaacggttt
    18901 ctcgggcgaa gttaagggta agtatgaagg ctctgtatct attaagccaa gcaaacgcga
    18961 ctacttatta gtttaattgc aaaaaaacca taaaattcga ccgtttgtag aaataacaag
    19021 cggtcttttt atatcagaat tttacaaaag gaacgaacaa tgacactgcg tgagaaactc
    19081 cttgctaata aaccaaaatt gcaacctatc gaaatcaacg gcgagaccta ctacctgcgt
    19141 gaagctactg ttggtgatat gaacaagcag atttttgaaa cccgaagctg gctcattcaa
    19201 caagctgaac aagaaaatgt tgaattaccg gcagaagatg atgaaacctt tgatgaggct
    19261 ctcaaccgtt ttggcgaaaa ataccgcctt gctcaatcgg ttgcctaccg cttatgtgat
    19321 gaaaacggtg tattactgtt taatcctctc gacattaacg atctgaatgc cattgccgaa
    19381 ttagacagta aagtgattat cgactttaac caagccgtgt ccgccccaaa agactcagcg
    19441 agcgaagaaa gttccaaata accctttcgc tcgcactggg taaaacactt gaagaaatcg
    19501 aacaaatgcc cgaacgccac tttgccgaat atcggctttt ctaccaagaa cagcctttcg
    19561 gcttatggcg agacgattac cgcaccgccc aacttgccca cttaaccgca atgttcaacc
    19621 gtgatccgaa aggcaaagct ccaaaattag cggagtttat gccgtttttt aatcggacag
    19681 atgaggtgga ggaaatagaa gatgatggtg tggcagatta tttggcgaaa aggtaaagaa
    19741 aacagcttga aattccttgc tcttggatat atatttcctc aaagagaagg agggaagtat
    19801 gtttgaagca ctagaccgtt ttatgattaa tacgattaaa attgcaatta ttgtattttt
    19861 ggtcgcttgc atctttatta gtatgccgaa ctggtggata gcttctatct tagtactttt
    19921 cccgactgcc tatttgcttg ataaatactg gtggcgaaat accgaaatgt atcgccgcaa
    19981 aatggataaa ttagttaaag agatgaaaaa taatcgtcaa atctaaatta acatcaattc
    20041 tagcccgctt tatgcgggct ttttatttgg ggtgaaaaag tgagtagctc aaacagatta
    20101 actattacct tagatgtaga aaattctaag ttactagctg gattcgcaca atctgataca
    20161 gtagcaaata agtttgctcg cagcttcatt gtgaatatgg atcaggctca gcaaaaagca
    20221 cgtcaatttg ctgatagatc gacaaaatat ctaaaaaata ttgaacaagc agcaaataat
    20281 cttaatagct ctgtatcaaa agaacgtttt tggcaaattg caaatcggtt gcaattcccg
    20341 acaagtaaag tgattagtta tgccaatagc tacaccgaac tcggtaataa actcaaatta
    20401 gtcaccgaaa gtgaatccca acacgcccga gcaatggcgg cggtgtatga tatttcgctc
    20461 aaaaccgccc aatctacaca ggcaacctct tcggtttatc agacctttgc tcaaaatgca
    20521 aaacagctcg gtatttctca aaacgatgtg gcaaaactaa cggaaacggt agccaaatcg
    20581 gttgctattt ccggtgcaag ttcggcaacc gcttcaaacg ccctagtgca attcagccaa
    20641 tcgttgttaa tgggtaagct caaagcccaa gaatttaact cattgatgac ccaaacaccg
    20701 tctgttattc aggcgattgc caaagggtta ggcattacca ctgccgaact taaagcaatg
    20761 gtggataacg gtgagatgtc tgctgaaaaa atgattgagg ggctaaaaaa agcagagagt
    20821 tatgtaaacg gacagtacgc caaaacaacc actacaatta gtggagcaat gcaaaatctt
    20881 tcaactgcgg ctgaaaagtg ggttggcgaa atggatagta ccgttggtgc gtcacagaaa
    20941 gccgttgccg ttctgaatgc gttaaatgct cattttgata cgttcgctaa aacagcactt
    21001 gtcgcaggtg gtgcatttgt gcttttcaaa ggctatatga gtgtacgccc atttatcgag
    21061 gctagagcgg cgatttatga gaatcgaaaa gcaattattg cggaaaatca ggctcgccaa
    21121 caaacgatag ctactcgtca gcaggaattg gttgtactct ctcaacaaac cgccttaaaa
    21181 ctggaacaaa cattacaaac ctacaatcaa aataaagccg aattagaggc gattgcggtt
    21241 aaacagcaga aaatcacaaa agaacgagaa gaaattgctg ttgctattca agcggcggca
    21301 actcgtacgg aaagattagc gttatctgct gaactgcaag cgctagatca gaaagaaatc
    21361 gctctcgggc aacagaaaat agcggttgaa cgccagcaaa ttgcaacaaa acaggctctc
    21421 aaagtggcgt atgctgaaaa tgcagttgct caaagtgcaa tgaatgcagg tatggtcact
    21481 gcaaccacaa caaccagagc gatgaatggg gttttagcgg ctgcaagagc tgaattgaac
    21541 ttactgaaag cagctgcact gtctaaccca ttaatgactc ttgcaatggt ggcaacgact
    21601 gccggtactg ccctttgggg attttttgaa agtcaaaaag cagctcgtga agaagccctc
    21661 cgctacgcgg acagcttaga ctccgttcga gccaatatcg aaaaaatgac taaggctcaa
    21721 actgatgcag aaatggttaa attgtctcgt tctatcaaag aacaggaaac agcattagct
    21781 agtctgaaac aacgccaagc tgaacttaca cgagaaatcg agcaaggagc tacggtttta
    21841 gatgatgcct ttggtgccgg tgtccgtttc gaaaaaagtg cgaaagagtt agcgaaagca
    21901 caagaagagt taaagttagt tactgctgat gttgaaaaag cacaaaatac attaaatgcc
    21961 agtttagaag ctcaagaagc cttacaagct cacgttccaa tcgctaacct acgagaggaa
    22021 tttgtcaaat tatatcctca cattgacgaa agccaaatca aagttgatgg cttaaatatt
    22081 gctatcggta attttaccgt caaatcaccg gagatggtta ttgccgctaa taacattgct
    22141 aatgctcttg gtggcgttgc cggagaggct atgagagcgg ctattctagt cgctaatctg
    22201 tcaagtatga agctggatgc taacggtgct gcaattatcg atcctaaaca tcttgaaaaa
    22261 atcgagcaaa ttgaacgtaa caatgcgatt gcttcggcta aaggcaaaga taaaatcgca
    22321 ttgcaagtaa aagacgctct gattaaatca ggtatgaaag agggcgatgc cggatacgag
    22381 cgactaaaag cagcatatga gcaacaattt acgttgcaaa atgcccctaa gcgcggcgga
    22441 aaaccgaaga aaaccaaagc cgaacgctct gccgaaagtt accaaaacca agtagcggag
    22501 atgaccaatc gtttatccgg cttaaagata gataaagaag atctcgtaaa atatggtggc
    22561 atttctcaat atcaggaagt gcgtaaactt actgaagata ttgctctgaa tgctgaaaaa
    22621 tataaaaact acggcactga aggcgtggct aaactcaaag agctggctgc taaaattgat
    22681 tctgaacaac agcttttaga cattgagcaa ttccaagtcg atcacgataa aaagctagag
    22741 gcaatgcaat tcgagctgga tttactggga aaaactcgta ctgaaaaaga attgttacaa
    22801 tttaacaatc aattagattt agaggccgag cggctgaaaa ttaatatgac gaaagagaat
    22861 attgctcttt tagatcagaa aattgccaaa attaaggaag aatacgagct acatcagaaa
    22921 aagcaggaag aaactcgtgc ttctgtaatg atgggaataa aagaaggctg gaacaatatt
    22981 gaaaccgatg tcagcaatgt ggcggcaaat gttgccaata ttactcaaaa tacctttgac
    23041 ggtatggcgg atagcttgac taattttgtg atgacgggta aagcggattt tcgtagtttt
    23101 gctaattcca tattatctga tcttagcaaa atgattatca aaatgatgtt atttaatgct
    23161 atcaaacaag gtgcaactat gttgtttggc taccaaggac cgactacaac ggtaaatggt
    23221 aatgctgttt atggattagg tacttgggca acaggtggct acacaggcaa tggcggcaaa
    23281 tacaccccag cgggtatcgt ccacaaaggg gaatacgtca tcaccaaaga ggcaacctct
    23341 cgtcttggtt tggattattt gaattatctt aactatggca gacgtggttt tgcctctggc
    23401 ggtggtgtcg ctgtaccaag agtaccgtca tcatcttatc agccgaaatc ggctcaaagt
    23461 agtatcagcg tacaggtcat caataatggt gagccggtag atgccaaagt cagccaaaaa
    23521 cagcaaggcg agcaaacgca aattactgtt gagttaatgc gtaaaattgc ccgtcaggaa
    23581 gcgaacggaa tgattcagaa caactttaga gccggaggag tatttgccta atggaaacat
    23641 tacaatggtg tgttcgcacc gatttagcgg ttgaaaatac ccctgaaata atggaagtca
    23701 aattcggcga tggttatacc caacgctcgc ccaaaggctt aaacaattta ttacgagcct
    23761 atacaggcac aatcaaagtg aaaaaggggg aacatttagc ggtggaggca ttttttgcca
    23821 aacatcgtgg tgtttcccct ttttacttta aagatccgta cacacaacaa aacaaaaagg
    23881 tagtgtgtag cagttggccc gcaaaaatgg ttggcttaaa ccattgggaa tttagtgtaa
    23941 cctttaagga agtaccctaa tgcctgttag catatccaat caaatgaaac tcgaccttgc
    24001 caaacttgag caaaatgcaa tgcttgattt gtacgaggta gatttacgca cattaaagga
    24061 taaaagcggt aatgctggct cagtttaccg cttttattcg gggttaaatg aactcaaaac
    24121 aagtattgtc tggcaagggc ggacttacga tccgtacccg attgaggcaa gtggatttga
    24181 acgaaacagt agcggcccaa gcaatcgccc tactcttacg ctatcaaatc tgtttggctt
    24241 agttacgggt attgctaatc agtttgatga gtgtatcggg gcgattgttc gccgtcatca
    24301 ggtttacgcc caatatttag atgccgtgaa ttttgccggg ggtaatgcga aagctgatcc
    24361 aaatcaggaa ataatcagtc attttgtgat tgagcagctt accagcctca cccgagaaac
    24421 ggcgaccttt acgctggcat taccgattga aactgataat gccaaaatcc ccagcagaat
    24481 tattatggcg gacacctgca cttggattta ccgctcggcg gaatgtggtt ataccggtaa
    24541 gca
//
LOCUS       DANLYI010000011        23935 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.011, whole genome shotgun sequence.
ACCESSION   DANLYI010000011 DANLYI010000000
VERSION     DANLYI010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 23935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23935
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.011"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            complement(156..1628)
                     /locus_tag="PXD81_000361"
     CDS             complement(156..1628)
                     /locus_tag="PXD81_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraG N-terminal
                     domain-containing protein"
                     /protein_id="HDL5536429.1"
                     /translation="MTFTVDSYLEYFLTLLAWIINNNIFAVLVQTGLFVLPLIFVLIS
                     TWMEVKKQGEDEGNKGDLLITWLSLKFYPAMFVVVLVLAPMIPINLNNIELNVERSKA
                     CGYRVPTAPEDSGYNQMNSELGDQQARVPIWWGLFHQINKGVTHALTASIPCKPDLRQ
                     VRFEVQHEKINNPALLTEVRLFVQQCYVPARQKIQNSQLSLSPAQVRETSWLGGRILI
                     ANSELYPRYRAQQPHSLFPYNASRDNGLPNNGEGGFPNCAEWWDTANNGLKDRLLDDV
                     KQNFSAKVIDFFSSKENSDESILRTVLRAENMDISRGKVYPGYGGNLDRSFTNEVTKT
                     ASMFGLSLGSIGAFPAFDSMRQALPMVHSFALMALIILTPLIMVMSGYSLKTLVTLTF
                     VNFALVTLTFWWELARWLDSWLLSVLYDSGTHSRINPYFLENTQDDIIVNFVMGSMFL
                     VLPAIWLGSMSWVGVKMGEFASHLTTGSRDSKTAGSKIGK"
     gene            complement(1639..1956)
                     /locus_tag="PXD81_000362"
     CDS             complement(1639..1956)
                     /locus_tag="PXD81_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012340821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemophilus-specific protein"
                     /protein_id="HDL5536430.1"
                     /translation="MKSDTTKPKMTRVITVLKITLMIIGIILLLLVVGWLAMTYFPST
                     NFQAWFYETRYAWLGWRFVLYVAIWLLMVQLNRKGQMPLKARLMILSVIPIIEGLNLL
                     YLL"
     gene            complement(1946..2374)
                     /locus_tag="PXD81_000363"
     CDS             complement(1946..2374)
                     /locus_tag="PXD81_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012340822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5536431.1"
                     /translation="MNKQHYRLFNAGPQKSSLGVVSPAVETQLLGFCEQIRQLIGVGK
                     QITLFPHRVVQNHWAIELSGEQGSVHLLLNVTGRSLLPTSNNSEWSARLIIEIADHVD
                     AYWFSAFLTQYLGLKSNLLPLSTSIADNSVYNEVIDNNEV"
     gene            complement(2374..5211)
                     /locus_tag="PXD81_000364"
     CDS             complement(2374..5211)
                     /locus_tag="PXD81_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012340823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer ATPase"
                     /protein_id="HDL5536432.1"
                     /translation="MSFLSEVFSSFKPTKAGVISSEPNENTGESDIIQNKMSEVNNEV
                     QQLKRSGKLTQSQHHRRLYSQEPSFIDHLPWGEYLNEHGVILLDDARSVGAVFDIKPI
                     GTAGRSIEFLSRIRNLVKDALQDSFDELDVNPYVVQFYCQDSDDLQPYIEQLRNYISP
                     IAKNSAFSEEWLRVQAKHFKDIGKPEGLFIDERVTNTAWSGRIRSTKMVIYRYVDKSN
                     DTNENLIVEQLNNACSRINGALTTAGLQLQRLDGEQFRQWLLRWFNPNPSINFEGLSN
                     NDCYHLFGKQTSNDGLPELAFNDFSEDLFYSQPKNEGNYWYFDDQPHEVVVVNNLRKQ
                     PEVGLMTGEITRGRNINALFDLLPESTIMAITIVIHPQDKLEAHLEKISSRAVGENFE
                     SKYLKEDCQTVQSYLKEGHKLYQAAIAFYVRGKDEKELRTRSRQLRTILLSNNLEPVK
                     ENAEIAPLNSYLRWLPMNFNPQMDAKSHYYTKYYFVQHLANLLPIFGRDTGTGNPGIT
                     YFNRGGAPFNFDPFNRKDRTKNAHKLLLGPTGSGKSATLCGQLCQAMAVYRPRTFVVE
                     AGNSFGLLADYAERFGLTVNRITLAPNSGIVLPLFSEAHRLLDMELNPEDVPEDIEDN
                     DDTDDEGDMQRDLLAEMELTARLMITGGEKKEDEKMSRADRAAIRRAILNAARQTKSE
                     GRQTLTEDIKNALEALSHDEHTREERRTRLAEMAESLEMFCTGVNGQFFNREGAIWPE
                     ADLTLVDLAMFAREGYEAELAISYISLINHINSLGEKYQHSDRMILNVTDESHIITAN
                     PLLAKFLVKGSKMWRKLGIWLWLATQNMDDFPNEAAKLLSMVEWWELLNVSASEIDEI
                     NRFRKLSEAQKTMLLSAKKSDRKYTEGVVLATNMEALFRVVPPSLFLALGMTEKHEKA
                     ERKQLMMQHGYTELDAALEVAARIDKARGIH"
     gene            complement(5214..5612)
                     /locus_tag="PXD81_000365"
     CDS             complement(5214..5612)
                     /locus_tag="PXD81_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012340824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03751 family conjugal transfer lipoprotein"
                     /protein_id="HDL5536433.1"
                     /translation="MKHLFCIMISALLITGCTTSTEELLPTNGSTMRDIWDKGASNND
                     LHLYRSQQGRTLDPVDYIPNSEQRAYTRTAENEINNLFPRLPNPDLIMYVYPHQTRSG
                     EPLSIPGYSTVLPFYGRVQYAQPGDRTRGL"
     gene            complement(5625..7079)
                     /locus_tag="PXD81_000366"
     CDS             complement(5625..7079)
                     /locus_tag="PXD81_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03752 family integrating conjugative element
                     protein"
                     /protein_id="HDL5536434.1"
                     /translation="MKANKALFVILGIVFILVGGIVYALFGRSESVPQPTQQEILDIS
                     IKDLTPEQVRTMGIQGDTPIDTLRTLVTTVKSNERRMDELIDENRQLKQKEDLVNGRI
                     NDAVNNETRKLQEQIADLKMQLFDMATLVNNKPTTQESTPQVEAQPNTVPNKGFGTAG
                     DKLPIGGATMSNVDTSGMYWVEPSDQISLDASGKPVSGNTVATKFGFPKDFNGGQSAG
                     NNEKKGLPFFPETDTVTSSQGTAGNARGKNQMTSVYTIPSNSTFLDSVAMTGLIGRIP
                     IDNRVSDAYPFKVLIGRENLIANGIELPDVDGAIVSGTATGDWTLSCVRGSVKSMTFV
                     FNDGRIVSTSNKGNGEEIGWLSDSSGIPCVPGERKTNAPEYLGTNFFLAAASAAAQGF
                     SQSQTTTVVDGSSVVGAVTGDQGKFILGQAIGGGLKESADWFRKRFAENFDAVYVPPG
                     HRVSIHIEKEIEIDYNAQGRKVKYGSKSTNSQLD"
     gene            complement(7091..7975)
                     /locus_tag="PXD81_000367"
     CDS             complement(7091..7975)
                     /locus_tag="PXD81_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012340827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03749 family integrating conjugative element
                     protein"
                     /protein_id="HDL5536435.1"
                     /translation="MKRLPILLVSLLIGFSGSSLADVLMKWERKPLPVALQVEKERII
                     FVDKNVKVGYPAELDGKLRIQSTGGAVYLKALETFPSTRIEFRDVTTGEIFLFDLTAA
                     SKVANPEEAIRVVFDGTVEKQSFSSNRDDESSDLPIANSRPQLPVPAALTRYAAQSLY
                     APLRTVEPLDGVRRVAHRLPKKITKLLPAYSIEATPLISWQLDEYVVTAIRLQNKGNT
                     QITLDPRELQGQFYAATFQHNWLGGYGENEDTTTLYLVTEGYANNALIPETRPVKLVS
                     KKVKRTTKVETKPAQSAK"
     gene            complement(7975..8619)
                     /locus_tag="PXD81_000368"
     CDS             complement(7975..8619)
                     /locus_tag="PXD81_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03746 family integrating conjugative element
                     protein"
                     /protein_id="HDL5536436.1"
                     /translation="MSQLKSQLHEKDKHIKTTRIFAFLFFIIILVLAGVIYTFPSRLT
                     IYNPPDLRAGSQRAWWEIPKPTIYAFSYQIFQQMNRWMTNGEEDYEKNITTLKPFLTP
                     HCEAFLRQDYKDRLRNNELRSRTRMVYEITGRGYSEDKVTIFSEDSWAVNLDLSVDEH
                     FQGQPVKRVYIRFPISIVRMDVDSAQNPWGLGFNCYHSIPKLLEGVEPKTEEKK"
     gene            complement(8623..8988)
                     /locus_tag="PXD81_000369"
     CDS             complement(8623..8988)
                     /locus_tag="PXD81_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03750 family conjugal transfer protein"
                     /protein_id="HDL5536437.1"
                     /translation="MSEQDTTIPFLPTRLNREATVYGGMTVSEFGISAGLGFMLGLIV
                     GLFLWILTDFWLLIPAMAMLLCIATVLIGKGIVAAVKRGKPEAYLNRLVEKKMDDLFN
                     GHKFIKREGFWSIRRYRRK"
     gene            complement(9011..9400)
                     /locus_tag="PXD81_000370"
     CDS             complement(9011..9400)
                     /locus_tag="PXD81_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2976 domain-containing protein"
                     /protein_id="HDL5536438.1"
                     /translation="MNRIKSLTKHLHSRYLAVMLFFWGSLQNAFAAGPDASKIPDFQI
                     PGVDSNTKDPMEIIWIVLKGLVTLGAVILAAVAILTVVKALIGIYNEATSENGKKGWG
                     HFILALIIGFIVIFFCLWLVKLAVGLF"
     gene            complement(9415..9648)
                     /locus_tag="PXD81_000371"
     CDS             complement(9415..9648)
                     /locus_tag="PXD81_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03758 family integrating conjugative element
                     protein"
                     /protein_id="HDL5536439.1"
                     /translation="MSYSPANAFSVASGIDPFRLKIMIAIIFLSLLLLAVAWALNSGY
                     KGFSFRDKSPFSLLVLFLKGVALLACVTYFFIY"
     gene            complement(9648..9974)
                     /locus_tag="PXD81_000372"
     CDS             complement(9648..9974)
                     /locus_tag="PXD81_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RAQPRD family integrative conjugative element
                     protein"
                     /protein_id="HDL5536440.1"
                     /translation="MSISTSLRKGIVVLVTLSCLPALASTEQEQLAQAIKQLEAAKLS
                     LQRAEKVAKASPKTREYFNYSAIHSDIETVKKGIQQYINPSRALPRDPNVLRTLSDDY
                     SKLRGQ"
     gene            10161..10544
                     /locus_tag="PXD81_000373"
     CDS             10161..10544
                     /locus_tag="PXD81_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012340830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5536441.1"
                     /translation="MRYQMRYLALFLVVFPYVAHSLPSAQKVGNICHAENAQYNYGGL
                     WELSLRGKLICNYWQFLGDDERKKIELQLDEYGEFPIWNTSEQISEQIIEDDEYPQYW
                     EVEDWEDSDWDTESENEDTEATTEE"
     gene            complement(10576..11271)
                     /locus_tag="PXD81_000374"
     CDS             complement(10576..11271)
                     /locus_tag="PXD81_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03747 family integrating conjugative element
                     membrane protein"
                     /protein_id="HDL5536442.1"
                     /translation="MSEETKARRKSGLINLIFGKINMSLAAIAVSLIFSIILEWIGIA
                     FWWPQEGHLHSQTMMLNEMQWLSDDFSQSLFAKSSTAFATSIIENVYNWLFVYTGVKE
                     WLTSPGNGSMEQWVHHYGRAYIESVIYVVITFIIRLIIIVFTSPLFLLTALSGLTEGL
                     SLRDIRKFGAGRESAYLYHHARRYIMPMMVTAWVLYLSIPFSIHPNIILVPAACLFGL
                     SICITAASFKKFL"
     gene            complement(11268..11621)
                     /locus_tag="PXD81_000375"
     CDS             complement(11268..11621)
                     /locus_tag="PXD81_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemophilus-specific protein"
                     /protein_id="HDL5536443.1"
                     /translation="MSSAKMREEKRTNLLDLPNKYRNFNGEFSASCGLDNAEELLIHS
                     QSYFIEWFEQGYSFHQFAEKFADQGLSLWSADEVSMRHSDKSKDIFAFYLAFDNNPSG
                     YILVQCQLDREDSLQ"
     gene            complement(11614..13815)
                     /gene="traD"
                     /locus_tag="PXD81_000376"
     CDS             complement(11614..13815)
                     /gene="traD"
                     /locus_tag="PXD81_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system coupling
                     protein TraD"
                     /protein_id="HDL5536444.1"
                     /translation="MSAKHTLEAWLRPPVEFFPAAVHASCAFVSAAAPWSLALHPSIG
                     YSIAAGFGALSFYRAKQGWQIVRYHRNLKRLPNYSMTSKQIPVSNKYLFVGKGFEWKP
                     IHTQRLHDCFSDNGSKYLEPSKLFKMAREYEKRHENWLTHLTSKDSRWNLVRPLPPVE
                     GIPAIHGIEPDEVDVVQSVSSRVGHTVVFGTTRVGKTRLAEVFVTQDIHRGKTADERE
                     VVVFFDPKGDADMLKRMYAEAKRAGREDEFYVFHLGYPEISARYNPVGRFNRISEVAG
                     RISGQLSGAGNSAAFKEFAWRFVNIVARALVELGNRPNYVQISQFVQNIDSLFLDYAE
                     KFIRKTDPKIWENLATIAAGLDEKALPFGMKDRPLIFVINKYIAESAVFDPILQGLAS
                     AVRYDRTYFDKIVASLLPLLEKLTTGKISELLSPDYADILDERPIFDWESIIRKRGIV
                     YIGLDALSDATVAAAVGNSMFADLVSMAGHLYKFGTNDGLPGAFKEKSKPVKINLHCD
                     EFNELMGDEFIPLINKGGGAGIQVTAYTQTLSDIEARIGSKPKAGQTLGNFNTLYMLR
                     VKEPATAELLTKQLHEVTIKRSTVTSSTTDSSNPDDDKAFSSNTGQRISESTVPMLVP
                     ANITNLPKGQAFALMDGSKLWKIRMPLPAVDKDDMMHQSLRELTDYMRKNYHVVENWW
                     QPAFTEYTPSRDIAEAFEAMSEPQYKTQIDHQVDNFGIDEPYDIDEEENDE"
     gene            complement(13815..14321)
                     /locus_tag="PXD81_000377"
     CDS             complement(13815..14321)
                     /locus_tag="PXD81_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012340834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrating conjugative element protein"
                     /protein_id="HDL5536445.1"
                     /translation="MQNKVKVLLFSLYVSHFSWADLTVIADFGGESAVRFYESLQPEH
                     DENAPNYPNSISASVSEADILPIVSHKLSPGTIKEKPFDLTGMQAIFLIGADNTSAQW
                     LQQNREKLITLNATGLVVNVQTLSELNQLRDIVPELRILPTPGDDLAERIGIAHYPVL
                     ITESGVSQ"
     gene            complement(14324..15094)
                     /locus_tag="PXD81_000378"
     CDS             complement(14324..15094)
                     /locus_tag="PXD81_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012340835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase"
                     /protein_id="HDL5536446.1"
                     /translation="MDITRVLVIVAVFLSNISISQAKPFNGFGSEIDSYIRMAAQRYQ
                     VSEAMLRGFVKMEDGWYGNISPTGATGVGQFTMGTWNWLSETERGKQLGMKLITAKNR
                     GSYADPRRDKYTNTLATALLARWHIEQFQLRNIPITDENLYMAHNIGLDGLHRAMMGK
                     ATKEDILNMRRNGMKSWMTVRDFLNYQKGRFLAHKQEANFMTVSKQGHIHTGVAAFVY
                     PTSQMGKSVKWVEPDNKMIWVEPKNAMVWVEPQNTLQR"
     gene            complement(15073..15846)
                     /locus_tag="PXD81_000379"
     CDS             complement(15073..15846)
                     /locus_tag="PXD81_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03759 family integrating conjugative element
                     protein"
                     /protein_id="HDL5536447.1"
                     /translation="MREISMKFNLCRKAIPLAVLPLLLSFALPTLAQNMQTETVSSSQ
                     STLQKQSQHQQSQLSDSLKQAKEWGLTVEEWSRYTELMKGERGMWSPNLDPLTALGIE
                     ARTAEEREKYARMLAKKYYERVSKELDFDKVYRQEFEKLYPNELPFEVEPHISQSVGR
                     VVYFTRFDNCDSCKSDLGKILSYANNQTPLDIYIVGQNISDNDIRKWAADNNIDPIKV
                     SKRLITLNHDKGYWLRYSLGKMPAAYQIKQDGEWTSLVY"
     gene            complement(15846..16475)
                     /locus_tag="PXD81_000380"
     CDS             complement(15846..16475)
                     /locus_tag="PXD81_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5536448.1"
                     /translation="MKTKISLMTVFMLGLSGCVHQQDTAQNSDEITPMPDSLLTPQMQ
                     QLPPQDRIVSKDIYQEQTTSNTEVVRDGRYTLISIAPEEGQKYLLEQVVSVKVSNPKN
                     RFTANVQQGLRSTLTNTGLDLCYPPIGEQGNAVQTLFTRPLPKVHYQFGPMKLYEALQ
                     MIAGPAYELTLNDISRTICFKARESSFQNPHKPAVAVTTTTIKTEVIEE"
     gene            complement(16634..16894)
                     /locus_tag="PXD81_000381"
     CDS             complement(16634..16894)
                     /locus_tag="PXD81_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5536449.1"
                     /translation="MTFELGRVCTTRAVAECFNQNALVELLNRYCYGDWGNLCEEDKL
                     ANQEAMKYGGRIVARYDVDDESLYIITEADRSCTTILFSYEY"
     gene            complement(17007..17369)
                     /locus_tag="PXD81_000382"
     CDS             complement(17007..17369)
                     /locus_tag="PXD81_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5536450.1"
                     /translation="MEDSFFDFDDISCYLGQWEAILEEYSDIVSIEDFWLVAKEFETV
                     PHFGNLYQELVISRLIQRFCTELDIEQDSDLVEFDYYINAIDTHFYINRQRICDIDDW
                     NEMLDKIRKEMTPAKLAA"
     gene            complement(17449..17856)
                     /locus_tag="PXD81_000383"
     CDS             complement(17449..17856)
                     /locus_tag="PXD81_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme utilization protein"
                     /protein_id="HDL5536451.1"
                     /translation="MNRQGIYINGKQVFDSKGKEINSVQIINSTVQTVVGINKGYISM
                     AAGNNVLIVDGQTIALEPSPTINIEVFGNVTNLNAGSENVRVTGDAKSVSTVSGDVTV
                     DGNVTSNVKTVSADIHCKSILGKINTVSGDIYR"
     gene            complement(17882..18562)
                     /locus_tag="PXD81_000384"
     CDS             complement(17882..18562)
                     /locus_tag="PXD81_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012340836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5536452.1"
                     /translation="MNSLQQFVKTLSPTVLNGRHFDGIVGDVVFVQNGYGKWLDQGFT
                     IIGFDKSIEEKDWIYLDWDCYWFGKNPAQVIPEKSWAQRSWLARAYSISLERFNSLGL
                     EREYNIVSDWDNPIYGTDIQLIEHSKEFFRWTEADDRGIFLVHNEMAFHFFKKEIKAS
                     SEPEYYQPIETFWKDATVVLNIEDLDMLERVGLEQTNTPALAKLTTFVQAARNEISNG
                     NIVFYRAF"
     gene            complement(18668..19099)
                     /locus_tag="PXD81_000385"
     CDS             complement(18668..19099)
                     /locus_tag="PXD81_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3577 domain-containing protein"
                     /protein_id="HDL5536453.1"
                     /translation="MTTQTSTQKNYFNLHTTGIGYLNDIRLVEHKKGNPFYACRVAAL
                     VGESDSPEYRYFDMNVVGKETETLIKKCQKAVSEEKKVLVSFVMADLWFDTFTYSSDS
                     KYHKKGDTGVSLKGRLIRIKMIKIDGEIVYQEKPKADEQSE"
     gene            complement(19917..21968)
                     /locus_tag="PXD81_000386"
     CDS             complement(19917..21968)
                     /locus_tag="PXD81_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012340838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HDL5536454.1"
                     /translation="MKLFICEKPSQARDLAGVLGATSKGEGLLATADKSIIVTWGFGH
                     LVEQYQPEDYDEAWKRWAFETLPIIPAQWKMTPKKESKKQYSVVIGLIKKATSVVIAT
                     DADREGEMIARELLDLAGYRGQIQRCWLSALDDASIRKALQTLKSGKETEALYYAGMG
                     RSRSDWLIGMNFSRLFTLMAQAKGYSGKPLSVGRVQSPTLTLVVNRDREIANFVPKSH
                     YALAVQLTTTSGETFVAGLAVPEQYLDESGLCLDSRVIQQAEAQIKQTGLAKVVTVET
                     KREKKSPPLLYALSDLQGECNRLFGFGAQQVLDIAQSLYEEHKITTYPRTDCGYLPES
                     QFSDVPNVIRSLVASDSGLQTLLPKLNLRQKSRAWDDKKITAHHGIIPTIKKADLSKL
                     SEEEMKVYDLIRRRYLAQFLPHLEMDKTVATLQSSGHTLVARGNVIVAQGWRILFGKN
                     VDEETGNDDEKQGLPVLTKNQQCNVTHSEVRTLQTKPPAHYTEGTLLDAMKNAARFVT
                     DPRLKQRLRETEGLGTEATRAGLIQGLIDKGFLLKKKKSLVASAEAVALIDSLPDLLK
                     NPGLTALWEQALNQIAEGSMTLDAFMQKQEAFVKQLIGSCMQQGMSLGNIEIRKCSEC
                     GKPMRKINHAKGTFWGCTGYPDCQHKEADKKVKSASNKSAKKSSNLNVLDQLNKLRSQ
                     L"
     gene            complement(22128..22646)
                     /locus_tag="PXD81_000387"
     CDS             complement(22128..22646)
                     /locus_tag="PXD81_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemophilus-specific protein"
                     /protein_id="HDL5536455.1"
                     /translation="MRYIKYSLFLVSTMLISGCAELSAINDKVGDWAGQMNQTLYGSK
                     DGSVEQHNGRQVYTKTRNGKYVYQDSGTLQLKRNIDDVFLKVRREFGFRPTNADSDSR
                     KVYHTVQGTFYHVSGYFESDVDTDGVHLGENYLEVIIEKGSKNQVNVEWKATGSARWL
                     DSAKQRLLNTLK"
     gene            23123..23833
                     /locus_tag="PXD81_000388"
     CDS             23123..23833
                     /locus_tag="PXD81_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5536456.1"
                     /translation="MWKKIRIDFTDTVKCQFCSRMITSGKAIVVQNELGNISFSGPSC
                     AQNKNGQNVVNPKEKIVDITKGCLLHEPTTNDKTDKQVDVEQDNFTSIHDDEVHNYLD
                     NNAVNAYLTLRFEKLSHLTSIIKSQKLNEIYHNYELTGQISQKDESYIRVIMYGEAYP
                     LYTYQNLQAVYAAEYWLQSFIQHNPENDLNFIQDTLNQLRRKLTLTEKQIMGINNWFK
                     NSDGKMVKLKTNAFVRGK"
BASE COUNT         7113 a         4954 c         4532 g         7336 t
ORIGIN      
        1 cagatttaaa atcacttttt tctgatgatg aagaacaaga agatgatgaa tatcaatatg
       61 ggtatgatgg accaggtata tatctaggta attaccgtgt tgatgagtaa aagtaaatag
      121 agtggtatat accactctat tattcagttg ttagattatt tccctatctt ggaacccgct
      181 gttttagagt ctctagaacc agtagttaaa tgacttgcaa actctcccat ttttactcct
      241 acccaagaca tactgcctag ccaaatcgct ggtaagacca agaacataga acccatcaca
      301 aaattcacaa taatatcgtc ttgtgtattt tcaaggaaat aagggtttat tctgctatgt
      361 gtgcctgaat catagaggac cgacagcaac caagagtcaa gccatctagc tagttcccac
      421 caaaatgtga gtgtaactaa tgcgaaattc acaaaagtta gcgtaaccaa tgtttttaac
      481 gaataaccac tcataaccat aattaaaggc gtgagaataa tcaatgccat taatgcaaat
      541 gaatgcacca tcggtaatgc ttgccgcatc gaatcaaaag cagggaatgc tccgatactg
      601 cctaatgaca gaccaaacat tgaagctgtt ttagtcactt catttgtaaa tgatctgtct
      661 aaatttcccc catacccggg ataaactttc cctctcgaaa tatccatatt ctcagctcgt
      721 aatacggtac gaagtatgga ttcatcggaa ttttctttac tgctgaaaaa atcaatcact
      781 tttgcagaga agttctgttt tacatcatct aataaacgat cttttaaacc attattcgcc
      841 gtatcccacc actctgcaca atttggaaaa ccaccctctc cattattcgg caatccatta
      901 tcccgagaag cattataggg aaatagacta tgaggttgtt gggctcgata acggggataa
      961 agctcgctat ttgcaatcaa aatacggcca cctaaccagc tagtctcacg aacctgtgct
     1021 gggctcaatg aaagttggct attttgaatt ttctgacgtg ccggtacata gcattgttgt
     1081 acaaataagc gaacctctgt caataatgct ggattattga ttttctcgtg ttgaacttca
     1141 aaacgaactt gacgtaaatc cggtttgcag ggaattgaag ctgttaaggc gtgtgttacc
     1201 cctttgttga tttgatgaaa taatccccac cagataggga ctctcgcttg ttgatcaccc
     1261 aattcagagt tcatttggtt ataaccacta tcctctggtg cagttggaac acgataacca
     1321 caggctttag aacgttctac attcaactca atattattca ggttgatcgg tatcattgga
     1381 gctaagacca atacaacgac aaacatcgcc gggtaaaatt tgaggctgag ccaagtaata
     1441 agtaaatccc ctttattacc ttcgtcctca ccttgttttt tgacttccat ccacgtactg
     1501 attaagacaa aaatgagagg cagaacaaac aaccctgttt gaaccaatac agcgaaaata
     1561 ttgttgttga tgatccaagc cagtaaggta agaaagtatt ctaagtaact atctacggta
     1621 aaggtcatct catcctcctt aaagtagata cagtaaatta agtccctcaa taattgggat
     1681 gacagataaa atcattaacc tggcttttaa tggcatttgc ccttttcgat taagttgtac
     1741 catcaatagc caaatagcta catataaaac aaatcgccat cccaaccagg catagcgggt
     1801 ttcatagaac cacgcttgaa aatttgtgga aggaaaatag gtcattgcca accagccaac
     1861 aacgagtagt aataaaataa taccgatgat catcaaggtt atttttaata cagtaataac
     1921 ccttgtcatt ttaggctttg ttgtatcaga cttcattatt atctattacc tcgttataca
     1981 cagagttatc cgcaattgat gtggataagg gtaaaagatt acttttcaaa ccgagatact
     2041 gagtaaggaa tgccgaaaac caataagcat ccacatgatc agctatttca ataattaaac
     2101 gagctgacca ttcactgtta ttggaagttg gtagcaaaga acgccccgtc acattaagta
     2161 ataagtgaac agaaccctgc tcaccagaaa gttcgatagc ccagtgattt tgcacgacac
     2221 gatgagggaa taaggtaatc tgtttcccaa cgccgatgag ttggcgaatt tgttcacaaa
     2281 atccaagcaa ttgagtttct accgctggag atacaactcc taatgaggat ttttgtgggc
     2341 ctgcattaaa taaacggtaa tgttgtttat tcattagtgt attcctcttg ctttatcaat
     2401 ccgagctgca acctctaaag ctgcatcgag ttcggtatag ccgtgctgca tcatcagttg
     2461 cttacgttct gctttttcat gtttttcagt cattcctaaa gctaaaaata agctaggcgg
     2521 tacaactcgg aatagagctt ccatattcgt tgctaatacc acgccctcgg tatatttacg
     2581 atctgatttt ttggcagata acaacatcgt tttctgggcc tctgacagtt tccggaaacg
     2641 attgatttca tcaatttcac tggcagacac attcaacaat tcccaccatt cgaccattga
     2701 gagtaatttc gccgcttcat tcgggaagtc atccatattt tgagtagcaa gccatagcca
     2761 aatccctagt ttacgccaca tttttgagcc tttaactagg aatttagcta ataatgggtt
     2821 ggccgtgata atgtgtgatt catctgttac attcagaatc atccgatcac tgtgttggta
     2881 tttttcacca agactgttga tatggttaat gagtgaaata taggaaatgg ccaactctgc
     2941 ttcatagcct tcccgggcaa acatagctaa atcaactaag gttagatcag cctccggcca
     3001 aattgccccc tctcgattaa aaaattgccc atttacaccg gtacaaaaca tttctaatga
     3061 ttctgccatt tctgctaaac gggtacgccg ttcttcacga gtatgctcat catgacttaa
     3121 tgcttccaaa gcatttttaa tatcttcagt cagcgtttgt ctgccttcag atttggtttg
     3181 gcgagctgca tttaaaatgg cacgacgaat agcagcacga tcagcacggc tcattttttc
     3241 atcttctttc ttttcacctc cggtgatcat caaacgtgcc gtcagctcca tttcggccaa
     3301 taaatctcgt tgcatatcac cttcatcatc agtgtcatcg ttatcctcaa tgtcttccgg
     3361 cacatcttcc gggtttaatt ccatatccaa tagacgatgt gcttctgaaa agagtggtaa
     3421 gacaatgccg ctgtttggag ctaacgtaat gcgatttaca gtcagtccga aacgttctgc
     3481 atagtccgct aataaaccaa atgaatttcc tgcctcaaca acaaaagtcc gtgggcgata
     3541 aacggccata gcctgacata attgcccaca taacgtagct gattttcccg aaccggttgg
     3601 acctaataat agcttgtgag catttttggt tctgtcttta cggttaaatg ggtcgaaatt
     3661 aaatggtgct cctcctcgat taaagtaagt gatccccgga ttcccagtcc cggtatctcg
     3721 gccaaagata ggtaataagt ttgccaaatg ttgtacaaaa tagtacttcg tgtagtaatg
     3781 tgatttggca tccatctgcg gattaaaatt cattggtagc caacgcaaat agctgttaag
     3841 tggggcaatt tctgcatttt ccttcacggg ttctaggttg ttactgagca gaatcgttct
     3901 caactggcga gaacgggtac gtagctcctt ttcatcctta cctcgaacgt aaaatgcaat
     3961 agctgcttga tagagtttat gcccctcttt aagataagac tgtacagttt gacagtcttc
     4021 ttttaggtat ttcgactcaa aattttcacc taccgctcga ctactaattt tttctaaatg
     4081 tgcctccaac ttatcctgtg gatggatgac gatggtaatt gccataatgg ttgattccgg
     4141 caacaagtca aataatgcat tgatattacg cccacgagta atctcgccag tcatcaaccc
     4201 cacttccggt tgtttcctca aattattgac tacaacaact tcgtgcggtt ggtcatcaaa
     4261 ataccaatag ttgccttcat ttttcggttg agaataaaat aaatcttcac tgaaatcgtt
     4321 aaacgctaat tctggaagcc cgtcatttga ggtttgttta ccaaaaagat ggtagcaatc
     4381 attgttgctt aacccctcaa aattaataga aggattggga ttaaaccaac gtaacagcca
     4441 ttgacgaaat tgttcgccat ccagtcgctg caattgtaat ccagcagttg ttagagctcc
     4501 atttatgcga gaacaagcat tgttcagctg ctcgacaatc agattttcat tagtgtcatt
     4561 ggatttatca acataccgat agatcaccat tttggtacta cgtatacgac cagaccaagc
     4621 tgtatttgtc actcgctcat caataaaaag cccctctggt ttaccgatat ccttgaaatg
     4681 cttggcttgt acccgtaacc attcttcgct aaatgcacta tttttagcga taggcgagat
     4741 ataatttcgt agctgttcaa tataaggctg taaatcatcg ctgtcttggc aataaaattg
     4801 aacaacgtac ggatttacat ccaattcatc aaagctgtct tgcaaggcat ctttgacaag
     4861 attacgtatc cgacttaaaa attcaatact acggccagca gtaccaatcg gcttaatatc
     4921 aaatactgcc ccaacactac gggcatcatc aaggagtatc acaccgtgct cattcaaata
     4981 ttctccccaa ggtagatgat cgataaagga gggctcttgt gagtataacc gacggtgatg
     5041 ctgtgactgg gtgagttttc cactgcgttt taattgttga acctcgttat ttacctcact
     5101 cattttattt tgaatgatgt cagactcacc ggtattttca tttggttcac ttgatataac
     5161 ccctgcttta gtcggtttga aagaggaaaa tacctcactt aaaaaagaca tgattaaagc
     5221 cctcttgttc tatcacccgg ctgagcatat tgaactcgtc catagaaagg tagtactgta
     5281 gaatatcctg gaatactgag aggctctccg gaacgagttt gatgtgggta cacatacata
     5341 atcagatccg gattaggtaa acgagggaac aagttattaa tttcattttc tgccgtacgg
     5401 gtataggctc gttgctcact attaggaata taatcgacag ggtcaagtgt tcgcccttgt
     5461 tgtgaacggt ataagtgtag atcgttatta ctcgctcctt tatcccaaat atcccgcatc
     5521 gtactgccgt tagtaggtaa taattcttct gttgatgttg tgcaacctgt tatcaataag
     5581 gcacttatca ttatgcaaaa taaatgtttc atcgtttgtt ccttctaatc cagttgtgaa
     5641 ttcgttgatt ttgagccata tttcacttta cgaccttggg cgttgtaatc gatttcaatc
     5701 tctttctcga tatgaataga gacacgatga cccggtggca catatacagc atcaaagttt
     5761 tcagcaaaac gttttctaaa ccagtcagca gattctttta agccgcctcc gatagcttgt
     5821 cctaagatga acttaccctg atcgccggta acagctccaa caacactact tccatcaaca
     5881 acagtggttg tttgtgactg tgagaatcct tgggcagcag cacttgcagc agccaaaaag
     5941 aaattcgttc ccaaatattc tggggcattg gttttccgct ctcctggaac acaagggata
     6001 cccgaactat ctgataacca gccaatctct tcgccattcc ctttattaga ggtactcaca
     6061 atacgtccat cattgaacac aaaagtcata ctttttacac tgcctctaac acaggatagt
     6121 gtccagtcgc cagtagcagt acctgataca attgctccgt ctacatcagg taattcaata
     6181 ccattagcaa tgagattttc tcgaccaata agtactttga acggataagc atccgatact
     6241 cgattatcaa tcgggatacg tccgatgagt cccgtcattg caactgaatc aaggaatgtt
     6301 gaattacttg gtatggtata tactgatgtc atctggtttt taccacgtgc gtttccagcc
     6361 gttccttgtg agcttgtcac agtatctgtt tccgggaaaa acggcagacc tttcttttcg
     6421 ttatttcctg cagattgtcc tccattaaaa tctttcggaa agccaaattt agttgctact
     6481 gtattgcctg acacaggttt tccacttgca tccaaactaa tctgatcgct tggttctacc
     6541 caatacattc cactggtatc cacgttggac atagttgcac caccgatagg taatttatct
     6601 cctgctgttc caaatccttt attcggtacc gtattaggct gagcctctac ctgaggggta
     6661 gactcttgtg ttgttggttt gttattaacg agagtcgcca tatcaaataa ttgcattttc
     6721 aaatctgcaa tctgctcttg cagttttcgg gtttcattat ttaccgcatc attgatacga
     6781 ccattcacta aatcttcttt ctgtttgagt tggcgatttt catcaattaa ctcatccata
     6841 cgacgctcat tggatttaac ggttgtcaca agggtacgca gcgtatcaat cggcgtatca
     6901 ccttgaatac ccattgttct gacttgttca ggtgttaaat ctttaatgga aatatctaaa
     6961 atttcctgtt gtgtcggttg tggaactgac tcactacgtc caaataaggc atagacaatt
     7021 ccaccgacta agataaatac aatgcccagt atgacaaata aggctttatt cgctttcatt
     7081 gcctttcctc ctacttcgcc gattgagccg gtttagtttc aacttttgtt gttcttttca
     7141 cttttttact gactagtttg actggacggg tttccggaat taatgcgtta ttagcatagc
     7201 cttcggtcac taaatacagg gttgtggtat cttcattttc tccgtacccc ccaagccaat
     7261 tatgctggaa agtcgctgca taaaattgtc cttgaagctc tcgagggtca agcgtaattt
     7321 gagtgttacc cttattttgc aaacgaatag cggtaactac atattcgtct aactgccagc
     7381 taattaaagg tgttgcttct attgaataag ccggtagcag cttggtaatt tttttaggta
     7441 gacgatgtgc cacacgacgc acaccatcta aaggttcaac tgtacgtagt ggagcataaa
     7501 gtgattgtgc tgcataacgg gtcaatgcgg caggtactgg aagctgagga cggctattcg
     7561 caataggcag atcagagctt tcatcatctc tattgcttga aaaagattgt ttttctaccg
     7621 taccgtcaaa aaccactcga attgcctctt caggattagc gactttcgaa gctgctgtta
     7681 aatcaaatag aaaaatttca ccggttgtaa catcacggaa ttctatgcga gtggagggaa
     7741 atgtttctaa tgcttttaaa tagacggctc caccggtaga ttgaattcgt agcttaccgt
     7801 ccagttctgc cggataacca accttgacat ttttatcgac aaaaataatt cgttcttttt
     7861 ccacttgtaa cgcaacagga agtggttttc tttcccattt catcaataca tcagccaagc
     7921 tactcccaga gaagcctata agcaaagaga cgagcaaaat tggtagacgt ttcattattt
     7981 tttctcctct gtttttggct caacaccttc taagagtttt ggaatggagt gatagcaatt
     8041 aaaccctaat ccccaagggt tttgagcaga atcgacatcc attctcacaa tactgatagg
     8101 aaaacgaata taaactcgtt ttaccggctg tccttggaaa tgttcatcca cacttaaatc
     8161 aagattgaca gcccaactat cttcactgaa tatggtaact ttatcttcac tatatcctct
     8221 accggtaatt tcatacacca tacgggttcg gctgcgtagt tcattgttac gcagacgatc
     8281 tttataatct tgtcgtaaaa atgcttcaca gtgtggtgtt aaaaatggtt tgagggtggt
     8341 gatatttttc tcataatcct cttcgccatt tgtcatccac cgattcattt gctggaaaat
     8401 ttgataggag aatgcataaa ttgttggttt aggaatttcc caccaagctc gttggctccc
     8461 agcacgcaga tctggtgggt tgtatatcgt aaggcgactt gggaaagtat agattacgcc
     8521 tgccagtacc aaaataataa tgaaaaacag gaaggcaaaa attcgagtcg ttttgatatg
     8581 tttatctttt tcgtgtaatt ggctttttaa ctggctcatt tgttatttcc ttctatatcg
     8641 gcgaatagac caaaaccctt ctcgttttat aaacttatga ccgttaaaga ggtcatccat
     8701 tttcttttca actaatctat ttaaataagc ctctggtttt cctcgtttaa ccgcagcgac
     8761 aatacctttt ccgattaaga cagttgcgat gcagagcaac attgccattg ccggaataag
     8821 aagccaaaaa tcagttaata tccacaagaa gagaccaaca atcagaccta acataaatcc
     8881 taatccggca gatatgccga actcactaac tgtcatccca ccgtaaacgg tagcttcacg
     8941 attaaggcgg gtgggaagaa aaggaatggt tgtatcttgt tctgacatat tggtctccta
     9001 gacattttaa ttagaagagt ccgactgcaa gtttgactaa ccacaagcag aagaaaatca
     9061 caataaagcc gataattaag gcgagaataa agtgtcccca acctttttta ccattttcag
     9121 atgtagcttc gttatagatg ccaatcaatg ctttaacaac tgtcaaaatg gctacggctg
     9181 ctaaaataac agctccgaga gtgacaagac ctttcagaac aatccaaata atttccattg
     9241 gatctttggt attactatcc acgcctggaa tttggaagtc tgggattttg gaagcatcag
     9301 gaccggctgc aaatgcattt tggagggaac cccagaagaa gagcattaca gccaaataac
     9361 gactatgcag atgtttcgta agagatttaa tgcggttcat aatagacctc gaatctaata
     9421 gataaagaaa taagtaacac aagctaacag tgccactcct ttgagaaata aaactaaaag
     9481 tgaaaaagga cttttatctc taaatgaaaa tcccttataa ccactattca atgcccaagc
     9541 gacggctaag agcagtaatg aaagaaaaat aatggcgatc atgattttca atctaaaagg
     9601 gtcgataccg ctggcaacag aaaacgcatt cgcaggggag taagacatca ctgcccccgt
     9661 aatttgctat aatcatcact taatgtacgt agtacattcg gatcacgagg taatgctcgg
     9721 cttggattga tatattgttg aattcctttt ttgacggttt caatatcact gtgaatagca
     9781 gaatagttaa agtattctcg tgttttaggc gaggctttag ctactttttc tgctcgttga
     9841 agtgataatt ttgccgcttc cagttgctta attgcctggg ctaattgctc ttgttctgtt
     9901 gaagcaagtg ctggcaagca agataaggta acaaggacaa caataccttt ccttaaagat
     9961 gttgaaatag acatagtgca cctctatttt tagttttact tcaggttaag agtgccaaaa
    10021 gcgatcctaa aatttaatgg aaaactctgt taccgttcag aagagaaatt gcacaaaata
    10081 gtaaatggtg agaaagatat tattctatgt ttaagctagt caattaagta gtatcaacac
    10141 cgaaatcttc acgctttacg atgagatacc agatgagata tctagcactg ttccttgtag
    10201 ttttcccata tgttgcccat tctcttcctt cagcacagaa agtggggaat atttgccatg
    10261 cagaaaatgc tcaatataat tacggaggtc tttgggaatt aagtttaaga ggaaagttaa
    10321 tctgtaatta ttggcaattt ctaggcgatg atgagcgaaa aaagatagag ttacaattgg
    10381 atgagtacgg ggaatttcct atttggaata cctctgaaca aatttctgaa caaatcattg
    10441 aagatgatga atatccacaa tattgggaag tcgaagactg ggaggattct gattgggata
    10501 ctgagtcaga aaatgaagat acagaggcaa caacagaaga ataagccccc gcatattgct
    10561 aagggcttat gaaatttaca agaatttctt gaaactcgcc gcagttatac agatggataa
    10621 accaaacaaa caggctgctg ggactaaaat gatgttcgga tgaattgaaa acgggataga
    10681 taaataaagc acccaagctg tgaccatcat tggcataata taacgtctag cgtgatggta
    10741 taggtaggct gattcccgcc cggcaccaaa ttttcgtata tctcgtaatg acagtccttc
    10801 ggttaaaccg gaaagtgcag tcaataaaaa gagtgggctg gtaaaaacaa taataatgag
    10861 gcgaataata aaggtgatca ccacataaat aacagattca atataggctc tgccgtagtg
    10921 atgtacccac tgttccatac taccattacc cggtgaagtt aaccactcct taacacccgt
    10981 ataaacgaat aaccagttat acacgttttc aataattgat gttgcaaaag cagtagaaga
    11041 cttggcaaac aggctttgag aaaagtcatc cgatagccat tgcatctcat tgagcatcat
    11101 cgtttggcta tgcaaatgcc cttcttgcgg ccaccagaaa gcaatgccta tccactctaa
    11161 aataatgctg aatatgagag atactgcaat tgcagcaagc gacatattta tttttccaaa
    11221 aatcaggttg attaaacctg attttcggcg agctttagtt tcttcactca ttgcaaacta
    11281 tcctctctat cgagctggca ctggacaagg atatagccgg aaggattatt atcaaaagca
    11341 agataaaaag cgaaaatatc tttggattta tccgagtgac gcatagatac ttcatcggca
    11401 gaccataacg aaagcccttg atctgcgaat ttttcggcaa actgatggaa ggaataacct
    11461 tgttcaaacc attcaataaa ataggattga ctatgaatca ggagttcttc tgcattatct
    11521 aagccacagg aagctgaaaa ctccccatta aaattacggt atttatttgg taaatctaat
    11581 agattggttc ttttttcctc tctcattttg gcactactca tcattttcct cttcatcaat
    11641 atcgtaaggt tcatctatgc cgaaattatc tacttgatgg tctatctgtg ttttatattg
    11701 aggctccgac atcgcttcaa atgcttctgc aatgtctcgg gacggcgtat attctgtaaa
    11761 tgccggttgc caccaatttt caacaacgtg atagttttta cgcatataat ctgtcagctc
    11821 tcgtaaagat tgatgcatca tatcatcttt atcgacagca ggtaacggca ttcgaatttt
    11881 ccataattta ctaccgtcca ttaaagcgaa tgcttgtcct tttggtaaat tggtaatgtt
    11941 ggccggaacc aacataggaa cggtactttc gctgatacgc tgtccggtat tggagctaaa
    12001 cgccttatca tcgtcaggat tactggagtc agttgtactg gacgtgaccg tgctacgttt
    12061 aatcgtcact tcgtgtaact gttttgtgag taattctgcc gtagccggtt cttttaccct
    12121 cagcatatag agtgtattga agttacctaa ggtttgtccc gctttcggtt tactgccgat
    12181 acgagcttca atgtcggaga gtgtttgtgt ataagcagtt acttgtattc cagcaccacc
    12241 gcctttgttg atgagaggga taaattcatc acccatcaat tcattaaatt catcacaatg
    12301 aagattaatt ttcactggtt tggatttttc tttgaatgct cccggtaatc cgtcatttgt
    12361 cccaaattta tataaatgac ctgccattga gactaagtca gcaaacatac tgtttcctac
    12421 agcggccgca actgtcgcat cagagagagc atctaaacca atataaacga ttcctcgttt
    12481 acgaataatg gattcccagt caaaaatcgg acgttcatcc aaaatatcag cataatccgg
    12541 ggataacagc tcagaaattt tccctgtcgt taatttttcc aataacggca acaatgaggc
    12601 aacaatttta tcgaaatagg tacggtcata gcgtacagca ctggcaagtc cttgtagaat
    12661 tgggtcaaag actgcacttt cagcaatgta cttgttgatc acaaaaatca gcggacggtc
    12721 tttcattccg aaaggaagtg ctttttcatc cagtccagcg gcgatggttg ctaaattttc
    12781 ccaaattttt ggatcggttt tacgaatgaa tttttccgca taatcaagga ataatgaatc
    12841 aatattttgt acaaactggg aaatttgaac atagtttggg cgatttccca attcaactaa
    12901 tgcacgtgcg acgatattga caaatcgcca agcgaactct ttaaatgccg ccgagttacc
    12961 cgctccactt agctgtcctg aaatacgccc agcgacttcc gaaatacgat taaagcgtcc
    13021 tactgggtta taacgggcag aaatttcggg ataacctaga tggaaaacat aaaactcatc
    13081 ttctcgcccc gctcgttttg cctccgcata cattcgtttg agcatatcgg catcgccttt
    13141 cggatcaaag aagactacca cttcacgctc atcagcagtt ttacctcggt ggatatcctg
    13201 ggtaacaaag acctcagcta aacgagtttt accgacacgt gttgtaccga atactaccgt
    13261 atgcccaacc cgagagctaa cggattgaac gacatcaacc tcatccggct caattccatg
    13321 gatagcagga ataccctcaa caggtggcag aggtctaacc agattccacc ggctatcctt
    13381 tgaggtaaga tgtgttagcc aattttcgtg tcgtttttca tactcacgag ccattttaaa
    13441 tagcttagaa ggctccagat attttgaacc gttatccgaa aaacaatcgt gtaaccgttg
    13501 agtgtgaata ggtttccatt caaatccctt accgacaaac agatacttat tactgaccgg
    13561 aatttgcttg cttgtcattg aataattagg caaacgtttc aggttgcgat ggtaacgcac
    13621 aatttgccaa ccttgttttg cccgataaaa gctcaatgct ccaaaaccag ctgcaatact
    13681 gtaaccgata cttggatgga gtgccaaaga ccaaggagct gcagcactga caaaggcaca
    13741 agaagcgtga accgctgccg gaaaaaattc aacaggaggt cgtaaccacg cctctaaggt
    13801 atgtttagca gacattattg tgaaactccg ctttcagtga ttaagacagg gtaatgtgca
    13861 ataccaattc gctctgcaag gtcatctccg ggagttggta atattctcag ttccggtaca
    13921 atgtctcgta attgattaag ttcggagagt gtttgtacat tcactacaag ccctgttgca
    13981 ttcaacgtga tcaacttctc tcgattttgt tgtaaccact gagctgaggt attatctgct
    14041 ccaattaaga aaatggcttg cattccggtt aaatcaaatg gtttttcttt aatagtcccg
    14101 ggagatagtt tatgggaaac aatgggtaaa atatcagcct cactcactga ggctgaaatg
    14161 gaattcggat agtttggtgc attttcatcg tgttccggtt gcaaagattc ataaaaacgt
    14221 accgcacttt caccaccgaa atcagcaatc acggttaaat cagcccaaga gaaatgggac
    14281 acatataaac taaacaataa tacttttacc ttgttttgca tttttacctc tgaagagtat
    14341 tttggggttc tacccaaacc attgcatttt taggttcaac ccaaatcatt ttattatccg
    14401 gctcaaccca tttgaccgat tttcccattt ggcttgtggg ataaacgaaa gctgcaactc
    14461 cggtatgaat atgcccttgc ttggagacag tcataaaatt ggcttcttgc ttatgtgcta
    14521 aaaaacgtcc tttttggtaa ttgagaaagt ctcttaccgt catccaactt ttcattccat
    14581 tacgacgcat atttaaaatg tcttctttag tagctttccc catcattgct cgatgcagcc
    14641 catctaagcc gatattatga gccatatata aattctcatc agtaataggg atattacgga
    14701 gttgaaattg ctcaatatgc caacgtgcca ataatgccgt tgctaaggta ttcgtatatt
    14761 tatctcgacg gggatcggcg taagaacccc gatttttggc tgtaatcaac ttcataccaa
    14821 gttgctttcc cctctctgtt tcagatagcc agttccaggt tcccatagta aactgcccaa
    14881 caccggttgc tcctgttggt gaaatgttgc cataccagcc atcttccatt ttgacaaaac
    14941 ctcgtagcat tgcttcactc acctgataac gttgagcagc catacgaata tagctgtcaa
    15001 tttcagaacc aaatccattg aagggctttg cctgagaaat gctaatgttg gaaagaaaga
    15061 ctgcaacaat gactaataca cgagtgatgt ccattcacca tcctgtttga tttggtaagc
    15121 agcgggcatt tttcctaacg aatatctcag ccaatagcct ttatcgtggt taagggtaat
    15181 caatctttta ctgactttta ttgggtcaat attattgtcg gcagcccatt tacgaatatc
    15241 attatcggag atgttttgcc caacaatata aatatcgaga ggtgtttgat tatttgcgta
    15301 agagaggatt ttcccaagat cacttttaca agaatcgcag ttatcaaaac gggtaaaata
    15361 aaccacacga cctaccgatt gagaaatatg tggttcaacc tcaaaaggta gctcattcgg
    15421 ataaagtttc tcaaactcct gacgataaac tttgtcaaaa tcaagttctt ttgacactcg
    15481 ttcataatat tttttcgcca acatacgagc atatttttcc ctttcctcag cagttcgtgc
    15541 ttcgatacct aaagcagtta acggatctag atttggcgac cacatacctc gctcaccttt
    15601 cattaattcg gtataacgac tccattcttc aacggtcaat ccccattctt tagcctgttt
    15661 taggctatca cttaattgtg attgttgatg ttggctttgt ttctgcaaag tcgattgtga
    15721 gctacttacg gtctctgttt gcatattttg agccagagta ggtaatgcaa aggacaataa
    15781 aagaggtagt actgccaacg gtatagcttt acggcataaa ttaaacttca ttgatatttc
    15841 cctcattatt cctcaatgac ctcggtttta attgttgtcg ttgtaacggc aacagcaggt
    15901 ttatgagggt tttggaatga actttcccga gctttaaagc aaatcgtacg ggatatatca
    15961 ttaagagtta gttcataagc gggacctgca atcatttgta gagcttcata taatttcatc
    16021 ggaccgaatt ggtaatgaac ttttggaaga ggtcgggtaa aaagtgtttg aaccgcatta
    16081 ccttgttctc cgataggtgg ataacacaaa tctaaacccg tattagttaa tgtagaacgc
    16141 aaaccttgct ggacattcgc agtaaaacga tttttaggat tggagacttt gactgatacg
    16201 acttgttcaa gcagatattt ttgaccttcc tccggagcaa tactgattaa ggtgtagcgt
    16261 ccgtcacgta ccacttcggt attcgaggta gtctgttctt ggtaaatatc tttggacacg
    16321 atacggtctt gtggaggtaa ttgttgcatt tgaggtgtta gcaatgaatc aggcataggt
    16381 gtgatttcat cactattttg agctgtatcc tgttgatgaa cacaaccact taagccaagc
    16441 ataaaaacag tcattaagga tatttttgtt ttcatttaat ctctccaagt atgaaaggaa
    16501 tctagaccaa ctatggcgaa gtcattgctg attttgaatg atttattctg ttgttgattg
    16561 agagaataaa aaaacgccat cattagacgg cgtttgaaaa cacaataatg tgctgtgtta
    16621 atcggatttc tccttagtat tcataagaaa ataagattgt tgtacagcta cgatcagctt
    16681 ctgtgatgat ataaagactt tcatcatcta catcgtagcg agctacaatt ctccctccgt
    16741 atttcattgc ttcctgattc gctaatttat cttcctcaca caaattgccc caatcaccat
    16801 aacagtaacg attaagcaat tcaaccaagg cattttgatt gaaacattca gcaacagcac
    16861 gagttgtaca tactcgacca agttcaaacg tcatacattt ctcctaaata aaatacgggg
    16921 aaatgtcacc cattagggga gtatttcccc aagtgggtgg actaagactg cggcaagagt
    16981 cgtttttagt aaaaatttgc aaaaaattag gcagctaatt tagccggcgt catttcttta
    17041 cgaatcttgt ctaacatttc attccaatca tctatatcac agattctttg gcgattaatg
    17101 taaaaatgcg tgtctattgc attgatatag tagtcaaatt caactaaatc tgaatcttgc
    17161 tcaatatcta attcagtaca gaatcgttga attaagcgag agatgacaag ctcctgatac
    17221 agattaccga aatgtggtac agtttcaaat tctttggcaa caagccaaaa gtcttcaata
    17281 ctgacaatat cgctatattc ttctaagatg gcttcccatt gtcctaaata gcaagagata
    17341 tcatcaaagt caaaaaagga atcttccatt atgtttctcc aagttttaaa taaaaagtgg
    17401 ggaaacatac ctaccgagaa tgtttcccga tagggtgtat agtcaatatt agcgataaat
    17461 atcaccgctc actgtgttaa ttttacctaa aatagattta caatgaatgt cagcactcac
    17521 agttttcaca ttcgaggtta cattaccatc cactgtgaca tcaccactga cagttgaaac
    17581 agatttggca tcgcctgtta cacgaacatt ttctgaacct gcattgaggt tggttacatt
    17641 gccaaatact tcaatgttga tagtcggaga gggttctaat gcaattgttt gtccatcaac
    17701 aattaataca ttattgccgg cagccattga gatataacct ttatttatcc caacaactgt
    17761 ttgcactgta ctattaatga tttgaacgct gttgatctct ttgcctttag agtcaaagac
    17821 ttgtttaccg ttgatataaa taccttgtcg attcatataa taatcctctt tgtattagat
    17881 gttaaaatgc tcgatagaat acgatattac cattggaaat ttcgttgcga gcagcctgta
    17941 caaacgtagt taattttgct aaagcaggtg tattagtttg ctctaatcct actcgctcta
    18001 acatatctag atcttctatg ttcaatacca ccgtagcatc tttccagaat gtctcaatag
    18061 gttgatagta ttccggttcc gatgaggctt taatttcttt tttgaagaaa tgaaaagcca
    18121 tctcgttgtg aactaagaaa atccctctat catcggcttc agtccaacgg aaaaactcct
    18181 tggaatgttc aattaactga atatctgtac cataaatggg gttatcccaa tcagacacaa
    18241 tgttatattc acgctctaag cctaacgaat taaaacgttc aagagagatt gaatacgctc
    18301 tagcaagcca agaacgttga gcccaagatt tttcagggat tacctgtgcc ggattttttc
    18361 cgaaccaata gcaatcccaa tctaggtaaa tccaatcctt ttcctctatg cttttgtcaa
    18421 aaccaataat agtaaagcct tgatcaagcc atttaccata gccgttctga acaaagacaa
    18481 catcgccaac aataccatca aaatggcgac cattaaggac tgttggtgat aatgttttga
    18541 caaattgttg aagtgagttc atcgtgtttc tcctaaaatt gtaaaataaa ggaaaacaca
    18601 ccgcagggag tgtttcccta cgggacataa agtaaagccc aacataaatt gtcgggcttg
    18661 ggtaaaatta ctcagattgc tcatctgctt ttggtttttc ttgatagacg atttccccat
    18721 caattttaat cattttgatg cggattaagc gacctttaag agacactcca gtatcgcctt
    18781 ttttgtgata tttactgtca cttgaataag tgaaagtatc gaaccacaaa tcagccatta
    18841 caaatgatac aagcactttt ttctcttcag agaccgcttt ttggcatttt ttgatgagtg
    18901 tttcggtttc tttaccgact acattcatat caaaatatct gtactctggt gagtcagatt
    18961 cgccaacaag agctgcaaca cgacaagcat aaaaaggatt tcctttttta tgctctacta
    19021 aacgaatatc gtttaaatag ccaatgccgg tagtatgtag attgaagtaa tttttttgag
    19081 tagaagtttg agttgtcata gtttgtttct ccaaatgtaa aaaagcggag aaacatcctt
    19141 acccaacacg ggaaaaatgt tccccgcaag gtggaagtaa aatggaatct caatgaatat
    19201 tcactgaaat gatagggatt gagttggcat tcctgcaaat ttacagagtt ccctttcaga
    19261 atgctgggag acattttggc aataactcaa ctacctctaa ggatagccgt cgcttctcag
    19321 gagttatgcg acttattaag tgctgttata tacagcgtct taaattgagg ataatttagc
    19381 catattgaat cataaagtta attgagaatt gaattttgat tttagagaat tggaaatgac
    19441 aacaccgttg ataaatcact taccaacggt gttgtacttt tgaagaataa atgaaattta
    19501 agttagacca atataagtta ctatattgaa aatagcagca ccttcgtgac aatgttgata
    19561 ttttcgttcg taatttaccc gattaccttg tggtctttaa tcggcatatc tgtggcatca
    19621 tcgaggacac aaacggcacc ctttattcac ctgtgggctc aggctcagaa tagttagcag
    19681 taattgcaac gtcataatcc ccccaatcgg gtaatattat gacgttgcaa tcacgattta
    19741 gacaattaca acagactaat gtggctggca ttatccattt aatgtttagt tggcattccc
    19801 acaaagtgga gttccctctc agaatgctgg gagacatttt ggctggtgta gattgataac
    19861 cgacaatcta caaaacgggt tctttgttcc ttgataatac cataatctac ttgaatttat
    19921 aactggcttc ttaatttatt cagttgatcg agtacattta aattactact tttcttagca
    19981 gatttatttg aagctgattt taccttttta tcagcctctt tatgttggca atctggataa
    20041 ccagtacatc cccagaatgt tcccttagcg tgattgattt ttctcattgg cttaccacat
    20101 tctgaacatt ttcggatttc aatattccca agtgacattc cctgttgcat acaactgccg
    20161 ataagttgtt ttacaaatgc ttcttgcttt tgcataaatg catcaagtgt catactacct
    20221 tcggcaatct gattgagggc ttgttcccat aatgccgtta aaccgggatt tttcagtaag
    20281 tccggcaaac tatcaattaa ggcaacggct tctgctgagg caaccagtga ttttttcttc
    20341 ttaaggagaa atcctttgtc aattaggcct tgaattaatc cggcacgggt tgcttctgtg
    20401 cctaaacctt ccgtttcacg taagcgttgt ttgagtcttg ggtcagtcac aaagcgagcg
    20461 gcatttttca ttgcatcgag taatgtcccc tctgtatagt gtgcgggagg tttagtttgc
    20521 aatgttcgga cttcagaatg agtgacattg cattgctgat ttttagtcaa tacaggtaat
    20581 ccttgtttct catcatcatt accagtttcc tcatccacat ttttgccaaa caagatacgc
    20641 cagccttgag caacgatgac atttccccga gcaaccagag tgtgcccact agattgcaga
    20701 gtggcaacgg ttttatccat ttccaagtgc ggtaagaatt gagcaagata tcggcggcga
    20761 atgagatcat agaccttcat ttcctcctca ctcaatttac ttaaatcagc ttttttaatg
    20821 gttgggataa taccgtggtg agcggtaatt tttttatcgt cccacgcccg acttttttgt
    20881 cgcaaattta atttaggcaa gagagtttgt agccctgaat cagaggcaac taatgagcgg
    20941 attacgttag gaacatcaga aaattgtgat tctggcagat aaccacagtc tgtacgggga
    21001 taagtcgtga ttttatgctc ttcgtaaagg gattgtgcga tatccaacac ctgctgagcc
    21061 ccaaagccaa acaagcggtt acattcgcct tgtaaatcac ttaatgcgta gagtaacggt
    21121 ggagattttt tctctcgttt agtttcaacc gtcaccactt tggctaagcc tgtttgttta
    21181 atttgagctt cggcttgttg tatgacacga ctatcaaggc aaagtccgga ctcatccaaa
    21241 tactgttcag gtactgcaag cccggcaacg aaagtttcac cagaggttgt tgtcagctga
    21301 accgctaacg cataatgaga tttaggcacg aaattagcaa tttctctatc tcgattaacc
    21361 acaagagtta atgttggact ttgtactcga ccaacgctca atggcttacc ggaatagcct
    21421 tttgcttgag ccattaaggt aaataaacgg gagaagttca ttccaatcaa ccagtccgag
    21481 cggctacgtc ccataccggc gtagtaaaga gcctcggttt ctttacctga tttgagtgtt
    21541 tgaagagcct ttcgaatact ggcatcatcc agagctgata gccagcaacg ttggatttga
    21601 cctcggtatc cagctaaatc taacagctca cgagcaatca tctcaccttc tcgatcagca
    21661 tcggtcgcaa tgaccacgga ggttgctttt ttgataagcc ctattacaac agaatattgc
    21721 ttcttagact cttttttcgg tgtcattttc cactgagcag gaataatggg taatgtttca
    21781 aatgcccagc gtttccacgc ttcatcataa tcttccggct gatattgctc aacgagatgc
    21841 ccgaaccccc aagtgacaat aatagattta tccgcagtcg ccagcaagcc ttcacctttt
    21901 gaagtagcac ccaatacgcc ggcaagatct cgagcttgag atggcttttc acaaataaat
    21961 aatttcatat tatttcctaa aatgaataca gataggcaaa tagtaatgtg ctgattaaat
    22021 agcattcaac gacttattgt ctcgtaggat gtaataattt gagaaataag gacataaaaa
    22081 atcccagtgt ttccactggg aagaggttct aggagcaata tgagttatta ttttaatgta
    22141 ttaagtaaac gctgttttgc actatcgagc catctagcac taccagttgc tttccattca
    22201 acattcacct gatttttact gcctttttca atgataactt ccaagtagtt ttcacctaag
    22261 tgtaccccat cagtatctac atcagattca aaatagccgg aaacatggta aaaagtacct
    22321 tgtaccgtat gataaacttt acgggagtca ctatcagcat tcgttgggcg gaagccaaat
    22381 tcccgacgaa ctttcaggaa tacatcatca atattccgct tcaactgcaa tgtgccggaa
    22441 tcttgataaa cgtacttacc atttcgggtt ttagtataaa cttgtcgccc attatgctgt
    22501 tcaacagagc cgtctttaga gccatagagc gtctgattca tttgcccggc ccaatcccca
    22561 accttatcgt taattgctga gagttcagca cagcctgaaa ttagcattgt agaaacaaga
    22621 aataaactat atttgatgta acgcatagaa ttctccttac aagcgggtta tctgcgttca
    22681 ttttttgcaa aagtgcggtt aaaatctact taaaactctt ttacttacaa agagaaaaaa
    22741 tattatagct ttctgtcaca cagtagacca tttagatcgg gtctgcttag ttgtgagatt
    22801 gttaatatcg cagagataag ttctaggcaa cgttcacagc tatggaaaat atgagtttga
    22861 gatgacatat gggatatacg cacattttcc cagtcatctg ggtatacaca tccgtgaatt
    22921 tcagtcaagt attgcttgat atgctcttct gtggcttgtt caacccagag ctctagctca
    22981 gtttgatact ggtgtaatat tttccatcga gttggaggga aattcatata tttatcctct
    23041 atcaaagtta tttttgcaat tatttcatca atgaagagag ttaagaagta tttttattac
    23101 tcactatatg gagagatatg ttatgtggaa aaaaattagg attgatttta ctgatacagt
    23161 gaaatgtcaa ttttgcagta gaatgattac atcaggcaaa gctattgttg ttcaaaatga
    23221 acttggcaat atctcttttt caggaccgtc ttgtgctcaa aataagaacg gtcagaatgt
    23281 agtcaatcct aaagagaaaa tagttgatat cacaaaggga tgtttacttc atgagccaac
    23341 aacaaatgat aaaacggata agcaagtaga tgttgagcaa gataatttca catccataca
    23401 cgatgacgaa gtacataatt atttggacaa taatgctgtt aatgcatacc tgaccttaag
    23461 atttgaaaaa ctatcgcatt taacctcgat tataaaatct caaaagttaa atgaaatcta
    23521 tcataactat gaacttacag ggcaaatatc tcaaaaagat gaaagttaca taagagtgat
    23581 tatgtatggt gaggcatatc ctttgtatac ataccaaaac ttacaagctg tttatgctgc
    23641 tgaatattgg cttcaatcat ttattcagca taatccagag aatgacttaa attttataca
    23701 agacacatta aatcagctca gaagaaagct aacattaact gaaaaacaga ttatgggcat
    23761 aaataattgg tttaagaatt ctgatggaaa aatggtgaaa ttaaagacaa atgcttttgt
    23821 aagagggaaa tagttaacat aattgtggtg gccacttatt ttgttacgta ggcccgacgt
    23881 aagactggtt ttaaaagact ctttcctatt atttgcttgc ttattttgac aaatt
//
LOCUS       DANLYI010000012        23528 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.012, whole genome shotgun sequence.
ACCESSION   DANLYI010000012 DANLYI010000000
VERSION     DANLYI010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 23528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23528
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.012"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            21..1166
                     /gene="lldD"
                     /locus_tag="PXD81_000389"
     CDS             21..1166
                     /gene="lldD"
                     /locus_tag="PXD81_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015674093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent L-lactate dehydrogenase LldD"
                     /protein_id="HDL5536457.1"
                     /translation="MIISSANDYRRAAKRRVPPFMFHYADGGSYAEQTLRRNVSDLED
                     IALRQRVLKDMSQLDTGIELFGEKLSMPVTLAPVGALGMYARRGEVQAAKAADNKGIP
                     FTLSTVSICPIEEVAPAIKRPMWFQLYVLKDRGFMKNALERAKAAGCSTLVFTVDMPT
                     PGARYRDMHSGMSGPYKDIRRVLQAMVHPFWAWDVGIHGKPHTLGNVSNYMGKPIDLN
                     NYIGWLTDNFDPSISWKDLEWIREFWDGPMVIKGILDPEDAKDAVRFGADGIIVSNHG
                     GRQLDGVLSSAKALPSIADAVKGEIKILADSGIRNGLDVVRMLALGADCTMIGRSFVY
                     ALSAAGQAGVENLLDIFLKEMKVAMTLTSNAKISDIGRDALVDLSNL"
     gene            complement(1220..1489)
                     /locus_tag="PXD81_000390"
     CDS             complement(1220..1489)
                     /locus_tag="PXD81_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5536458.1"
                     /translation="MLSEERFGFFYIFIFIYFSLLSLSIGYIRPELRTTYDYLEIENK
                     RYYVLSIHRNNIYVLGEKTKDNDEFLFFNQDTLKYYRINITKMPN"
     gene            complement(1564..1950)
                     /locus_tag="PXD81_000391"
     CDS             complement(1564..1950)
                     /locus_tag="PXD81_000391"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5536459.1"
                     /translation="MTNQKFINSSLTLAFFTLISFSTAYCYAWGIAAFHGYPWWHVEV
                     GNAGIARALAYVFGTFLTIFLFYLIGYALVNKVFKLHYFKYLGWLRVSVLVTIFSLPV
                     MISFYLFIGKVPMYLHLLYLGTTTSI"
     gene            complement(2203..3033)
                     /gene="fghA"
                     /locus_tag="PXD81_000392"
     CDS             complement(2203..3033)
                     /gene="fghA"
                     /locus_tag="PXD81_000392"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014167586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HDL5536460.1"
                     /translation="MQQFENYRCFSGEQQVWQHFSASLNCEMKFAIYLPQSAKTQKLP
                     VLYWLSGLTCTEQNFITKSGFQRYADEHHLIVVAPDTSPRGVNVANDEAYDLGQGAGF
                     YLNATQGEWTTHYQMYDYIVSELPTLINAHFPTNGKQSIFGHSMGGHGALTIAFKNPG
                     QYQSVSAFAPIVAPTQVPWGQKAFLAYLGEDQTAWKAYDSVALLEKYRPSLPILIDQG
                     SQDSFLSDQLKPEIFCETADRLGIPYQFNLRDGYDHSYYFISSFIGEHIAFHAKHLKD
                     "
     gene            complement(3113..4171)
                     /locus_tag="PXD81_000393"
     CDS             complement(3113..4171)
                     /locus_tag="PXD81_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HDL5536461.1"
                     /translation="MAYPIKSYATFSATTPLAPHSFERRELLEDDVLIDILYCGVCHS
                     DLHMARNDWGWTQSYPLVPGHEIIGRIIKVGKNAKKFQIGDLVGVGCMVDSCRECSPC
                     QHGLEQYCEVGNIGTYGGIDRFDGTPTQGGYSTCVVVSEDFVLKVSENLDTKAVAPLL
                     CAGITTYSPLRHWNVKAGSKVAVVGLGGLGHMAVKLAKAMGAEVTLFTRSLGKADDAY
                     RLGASRVVLSTDEKQMADVANTFDLIIDTVPYTHDLKPYIPTLALDGTLVLVGLVGEL
                     EQTINTVPMILGRRSISASVIGGIQETQEMLDFCAEHNIVPDVEMIDMQNINDAYERM
                     LKSDVKYRFVIDMGSLKG"
     gene            complement(4358..5293)
                     /locus_tag="PXD81_000394"
     CDS             complement(4358..5293)
                     /locus_tag="PXD81_000394"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03571 family LLM class oxidoreductase"
                     /protein_id="HDL5536462.1"
                     /translation="MNTLSPIFSQVFGQNQLTLGLIAPFMGYQNHIPDNLTTIADNAR
                     LAENLGFSALWVRDVPFYDPHFGDVGQGLDPMVTLGFLSAHTQNIALGTAGLIAPLRS
                     PIHIAKMATSVDSLSHGRFLLGLSSGDRPVEYPAFGADFASRSERFRESIDLINTLTE
                     QDFPHFKGKHYADLTDNLDLLPKPKHRLPIVAIGRARQEMDWLANVPDAWIWHGVNPA
                     DTANIINTLADLRQKDTPTAFGYAQFVEVLDDKNAKAQLFNNIYLRGGAKGLADFWAK
                     EQEKGVKHLALNLKPTQRPADEVLTELAEYALTQF"
     gene            complement(5573..5884)
                     /locus_tag="PXD81_000395"
     CDS             complement(5573..5884)
                     /locus_tag="PXD81_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDL5536463.1"
                     /translation="MSETEKQAINAPNAVMNGYLTMHYPDWFKPDGIYFNDGAFESFE
                     SSHKLTKDGKIRLVPTAGHTLGHLAVVVDMGEHYILIGGDASYSEQDMLAGNIDGVCN
                     A"
     gene            complement(5891..6283)
                     /locus_tag="PXD81_000396"
     CDS             complement(5891..6283)
                     /locus_tag="PXD81_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDL5536464.1"
                     /translation="MIKVHCLTTGWVQIKIHHQVARFFARPARVLDVLTDKKWSPKLP
                     IGCWLIEHDDGLILVDTGESSRANDKGYQPWWHPFMQFCERRGVKKHEEVGEILKANG
                     FDPLRIDTVVMTHMHGDHAGGLPNFPNS"
     gene            6415..6975
                     /locus_tag="PXD81_000397"
     CDS             6415..6975
                     /locus_tag="PXD81_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HDL5536465.1"
                     /translation="MNDLRVIKTHLAIQNALIELLDEKLFEKVQVQEIIEKALINRTT
                     FYRYYSGESDLVGKLIKEVKAEYQDLLKKRFESGNLLRFMQTIGNRLLEKRRLILALW
                     QIKTPRHHLYDDMHMLLKNAFIAHAKRITSDDKNWAEKNWDYQAHIFATIALESHKYY
                     FEQGKLLPIPQVFEEWIEMVQVLKSV"
     gene            complement(7041..8030)
                     /locus_tag="PXD81_000398"
     CDS             complement(7041..8030)
                     /locus_tag="PXD81_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012073273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HDL5536466.1"
                     /translation="MSKQIQFTKTGSPDVLQIVDVAIPAPKAHEVQIQIQAIGLNRAE
                     MMYREGAYVIDPVFPATLGYEGAGVIVAIGEGVNEVAISDKVSIIPSFMFTEYGTYGE
                     IVNMPKHAVVKHPDNLSMEQASASWMAFVTAYGGLIEFGKVQKGDFVVLGGATSSVGL
                     ASIQIAKMQGATVIALSRTHAKGNVLLEKGADFVIATSEDDVTAKLFEITNGKGVNVV
                     FDPVGRKEAAKIINAMAQDGRYIIYGALSHDDIAVPVFPILGKHLTVRGYELFEITTV
                     PEKLAQAKTFVYDGLASGQLRPEIDKIFAFDEMVKAHEYMASNQQIGKIVVKV"
     gene            complement(8244..9161)
                     /locus_tag="PXD81_000399"
     CDS             complement(8244..9161)
                     /locus_tag="PXD81_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDL5536467.1"
                     /translation="MTFIIGILAGVLIAFQSAINARLRMHVGAAFLTSFVSFSVGLLF
                     LLGLSLIMQQTPAFSLDNLGNVPLWAWTGGLLGMVGLTANVLIFPKLGGVQTAVMPIL
                     GQVLMGVLIDTFAWLNSPYIAFTASRLFGIGLVLFGVWIAVVLPHLSGLKRKENANLW
                     LWRAVGILGGTALATQAAVNGEFGRQPGSSLGAATVSFGVGAIGLLLVVLFYEKSLPK
                     LCQPTEGKPFWIWSGGILGSLFILAGVWLVPQIGTGSVVMLVLSGLICGSLLVDKFGL
                     FGITQKPILPLQIIGVTLLLAGVAFIRLL"
     gene            complement(9297..9956)
                     /locus_tag="PXD81_000400"
     CDS             complement(9297..9956)
                     /locus_tag="PXD81_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003672168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HDL5536468.1"
                     /translation="MQFFNKEDVINAFHYRASTRSYDGSRKIPAEDFNYILELGRLSP
                     SSVGSEPWQFLVLQNPELRQKIKPYCWGIPTMETSSHIVVILAKKNARYDSQYFSEIM
                     DRRGLQGEAREKAMAVYKKFQEKDIGILDSERSLFDWASKQTYIALGNMMTGAALIGV
                     DSCPIEGFAYQEVNDILAAEGLFDPSEWGVSVMCTFGYRDKEIRQKARKDFETVVKFI
                     E"
     gene            complement(10141..11205)
                     /locus_tag="PXD81_000401"
     CDS             complement(10141..11205)
                     /locus_tag="PXD81_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase/NADH oxidase"
                     /protein_id="HDL5536469.1"
                     /translation="MAKFRKLFTPFNIKNLALKNRVVMPPMCQYSATNGVPNDWHFVH
                     YTSRAVGGVGLIIVEMTNVAPNGRITPNCLGLWNDEQQQAFKKLVDSVHAQGAKIGVQ
                     IAHAGRKAQDEPDAVAPSAIHYGELDYAGQNLITPRELTTDEVKALVQAFKDSVKRAV
                     EAGFDTIEIHGAHGYLIHQFYSPKSNQRSDEYGQDKMLFGEQVIQAAREVMPSEMPLI
                     VRISAQEYGENGFGLDYGLQVAKRFADAGADVLDISGGGDGVLHAGNHPEFYAGYQVD
                     FARQVKLATGKPVIAVGMLDNPAVGDHILGMGDADLVAVGRALLRDPYWVLNAQYDQN
                     AADSSEMQFVPKQYERGLFM"
     gene            11296..12204
                     /locus_tag="PXD81_000402"
     CDS             11296..12204
                     /locus_tag="PXD81_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDL5536470.1"
                     /translation="MSDIRTLDFNLLKAFVVLLDECNVSRAAQRLSITQPAMSGILNR
                     LRESFNDPLFVRVQHGMQPTDRALQLGQTARNILQDISSMLQPPILEPEHLNMTLRIA
                     AMDYVQQIIALPLILRLRRLAPNVKVALLPVQGQNIKTLFEQKKIDLALVSRQHLSTD
                     TLKTVLYEERYVCAMSKTHPMAQTPLSLDQFCNLPFAMLSYNGGEFSGATDLALQKIG
                     LQRKVMVSVNHISLLPQLLQGSDLVAVLPERLAKTLPNVYLQPPPIEVEGFTMMMAWH
                     ERTEQDTAHRWLREVLQGVVKPKQAL"
     gene            complement(12274..12825)
                     /locus_tag="PXD81_000403"
     CDS             complement(12274..12825)
                     /locus_tag="PXD81_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HDL5536471.1"
                     /translation="MAKQIAVLIGSGSKTSFSHLTVRHLQKMAPASIQLNIVDIADLP
                     LYDRDLDENSPAQYTRVREAVAVADGVLLVTPEHNASFSAMLKNAIDVVSRPMGQSKW
                     IGKPAGIVTVGASPAGGVRAADQVRTITSGSFVNMPTLPFALNVGNLFTGVFDEQGEI
                     ASETLKSSMQNFINAYAQFVEKY"
     gene            13017..14378
                     /locus_tag="PXD81_000404"
     CDS             13017..14378
                     /locus_tag="PXD81_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HDL5536472.1"
                     /translation="MLFLNKIKQLFIFAFTLVLSACSLVVYKPVETIKQVNVERGYRQ
                     KNVIAESAKDGNIVVIMFSGGGTRAAALGYGVLEEFKKVRVKETNKGNTLLENIDIAF
                     GVSGGSILATYFALEGKDIIPKFADRFLKANFQKDIITEVFSLSNLPRLSSPQYGRGD
                     LLQERLNFALYHGKKFGDLVKNRKGPFAVISATDMNIGQKLTFTQETFDGLCLNLNDL
                     EISRAVAASSSVPLIFAPLTLNNNGGNCHFSMPQEFSAREKAIDGLITKNIEETNHIL
                     SLYQDSKERPFIHLVDGGLTDNLGLASVLEISDVMGIEEMYQYIHQLRVKNIVVINVN
                     AQNEVSSEIDKTANVPGVADVVNTIVNVPIDRTTQINLQRYRQFTDGWNEYANKQPSK
                     IEMHFVSLGLKELPDGDLKKDVLNIATSFYLPEEDVDKLREAARVLLQQSQEYNAALK
                     TLQ"
     gene            complement(14426..14923)
                     /gene="rraA"
                     /locus_tag="PXD81_000405"
     CDS             complement(14426..14923)
                     /gene="rraA"
                     /locus_tag="PXD81_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014992278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HDL5536473.1"
                     /translation="MRIDTSALCDLYSDQVDVVEPIFSSFGGASSFFGKITTVKCFES
                     NGLIAEVLEEEGEGRVLLVDGGGAVRRALIDAELAQLAVDNGWEGIIVYGAVRQLDIL
                     ERLDIGIHALAPIPVGADNTDIGEVDTPVNFGGVTFFPEDYVYADLTGIILSQELLDL
                     NDISE"
     gene            complement(15021..15923)
                     /locus_tag="PXD81_000406"
     CDS             complement(15021..15923)
                     /locus_tag="PXD81_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HDL5536474.1"
                     /translation="MTEKVTETAPNIDVVAETEKVIHKVSNMNIETMLNEWIIPYGTK
                     ILLAIAIFVVGKFLARLISRLLGKAALKSTKDEMLQSFIVSISYFLLLLIVVIASLSQ
                     IGINTSSLVALIGAAGLAIGLSLQNSLQNFAAGVMLLIFKPFRKGDLIETGGMTGVVE
                     QMGLLVLELRTGDNKTVLLPNGKVFSDSIINYSNNPTRRIDFTFDISYESNLKEAKEI
                     IQRILDANTYVLKDPAPVVAVGALAPHSVQLVVRPWVQTPNYWAAYWEIIEQVKLAFG
                     EAGISIPYNQMELHIKSGSLENKA"
     gene            16032..16994
                     /gene="rluC"
                     /locus_tag="PXD81_000407"
     CDS             16032..16994
                     /gene="rluC"
                     /locus_tag="PXD81_000407"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HDL5536475.1"
                     /translation="MTTEKVISAGVQFFTISEDEAGQRLDNFLLAKLKGVPKSLIYRI
                     VRKGEVRVNKGRNKPEYKLQPDDLVRVPPVRVAEKNEAPISTKLNKVAELESQILYED
                     ESMLVINKPSGIAVHGGSGLSFGVIEALRALRPQARFLELVHRIDRDTSGILLVAKKR
                     SALRSLHEQLREKVVQKDYLALVRGQWQAHTKVIKAPLLKNELASGERIVRVSEEGKP
                     SETRFSIEERYANATLVKASPITGRTHQIRVHTQYAGHPIALDDKYGDAEFDSKMKEV
                     GLQRLFLHAHAIRFEHPKTGEEMVITAPLDKTLKGVLAKLRANY"
     gene            complement(16977..17114)
                     /locus_tag="PXD81_000408"
     CDS             complement(16977..17114)
                     /locus_tag="PXD81_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5536476.1"
                     /translation="MDLHIFAYILLVQASTLVPLYVDLQHKRGRLRYLTLSLSYLVIS
                     P"
     gene            17103..17960
                     /gene="folP"
                     /locus_tag="PXD81_000409"
     CDS             17103..17960
                     /gene="folP"
                     /locus_tag="PXD81_000409"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HDL5536477.1"
                     /translation="MQIHFKNFQKNDRLLDLTTPQIMGILNFTPDSFSDSGKFFSLDK
                     ALFQAEKMLADGASIIDIGGESTRPNAAIVSLEQELERVVPVVEAIRSRFDCLISVDS
                     SKAEVFKESAKVGMDIINDIRALTEPNALAAAVELGLPVCLMHMQGNPQTMQQNPHYD
                     DVLEDVADFLNQRIFECITAGIPKEHIILDPGFGFGKSVKHNYQLLKHLKAFVDSGFP
                     VLAGLSRKSMIGAVLDKPVEQRLLGSVVGALLAAQNGAKILRVHDVAETADALKIWQA
                     MLESDNEEK"
     gene            17960..18307
                     /locus_tag="PXD81_000410"
     CDS             17960..18307
                     /locus_tag="PXD81_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HDL5536478.1"
                     /translation="MTTLVYGIKNCDTVKKALKWLEDHNQNPQLHDYRVDGLNADWLV
                     EMEAKFGWENLVNKRSTTWRNLDEEIKNNLNRESALKVLTEQPTLIKRPIVIKEDIAL
                     IGFNEKEYVAAFN"
     gene            18437..19585
                     /gene="dapE"
                     /locus_tag="PXD81_000411"
     CDS             18437..19585
                     /gene="dapE"
                     /locus_tag="PXD81_000411"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HDL5536479.1"
                     /translation="MKNEIISLAQNLIRRDSISPQDKGCQQEIAQRLEKLGFNIEWLP
                     FGDTLNLWATHGSENGKTEPCIVFAGHTDVVPVGDESQWAYPPFSAEIVDGMLYGRGA
                     ADMKGSLSALVVAAETFVQHHPNHKGKVALLITSDEEAAAKDGTVKVVETLMARNEAV
                     HYAVVGEPSSSKVFGDVIKNGRRGSITADLYIEGIQGHVAYPHLAENPVHTSLGFLTE
                     LTTYQWDNGNEFFPPTSLQIANIKAGTGSNNVIPGELYVQFNLRYCTEVTDEIIKAKV
                     AEMLEKHGLKYRISWNLSGKPFLAGNGKLVEAALQAVENVTKITPRLDTGGGTSDGRF
                     IALMGAEVVEFGPLNATIHKVNECVSADDLAHCGEIYYQILEKLLATA"
     gene            19696..21030
                     /locus_tag="PXD81_000412"
     CDS             19696..21030
                     /locus_tag="PXD81_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HDL5536480.1"
                     /translation="MQLYSYICLLFGLAMFISFFTRKLNENIQTTIAITASALVGSLL
                     ILTFGSLGWFNVDKLAISIFEQLDFKSFLLNGMLGFLLFAGSLGIKLPLMKEQRREIA
                     VYALFSTLVSTFLIGALIYGVARLLGLELGFVYCLLFGSLISPTDPIAVLAIIKSLKA
                     PKRLSMHVEGESLFNDGIGLVIFTTIFAVAFGGQEPNLSGITGLFLQEAVGGIVFGLL
                     TGFVAHKFISATDDGSLEILITLTIPTVGFVIANYLHVSGALTMVVAGIMIGNWTRRS
                     GFSEQSQKYLDHFWEMIDHSLNYLLFLLIGLSTLLVDFTFIGGVLMLAAIPICLLARY
                     ISLWIPYQVLKRFRTYNPYTLKIMTWGGLRGGLALAMALSVPAGAAVVSSSEGNLDVR
                     DLILLMTYAVVTFSILVQGSTVEPMIKKSKLVDPRKVALNKLGAPDDVIPHI"
     gene            21137..21646
                     /locus_tag="PXD81_000413"
     CDS             21137..21646
                     /locus_tag="PXD81_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE family protein"
                     /protein_id="HDL5536481.1"
                     /translation="MLFTGLLCGFLLGFVMQRGRFCITGAFRDLYVTKNSRMFVALLI
                     AITVQSIGIWLLYEAGSFSSPAEDLPLLAVIIGAFLFGIGIIYAGGCATGTWYRAGEG
                     LIGSWVALVIYGLFSASMRTGVLAPLNQELKSNVIQHRTIYETFGISPWVLVFILSVI
                     TLALSAHSI"
     gene            21675..21896
                     /locus_tag="PXD81_000414"
     CDS             21675..21896
                     /locus_tag="PXD81_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HDL5536482.1"
                     /translation="MNVRLPASGLVCPFPLVEAKAAMAKLNKGDSLTIEFDCTQATEA
                     IPNWAEEEGYEVTDYQQLGDALWEITVVK"
     gene            complement(21961..23469)
                     /locus_tag="PXD81_000415"
     CDS             complement(21961..23469)
                     /locus_tag="PXD81_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012478468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase"
                     /protein_id="HDL5536483.1"
                     /translation="MSKCPVTHLTMNNGAPVVDNQNSLTAGPRGPLLAQDLWLNEKLA
                     NFVREVIPERRMHAKGSGAFGTFTVTNDITKYTRAAIFSQVGKKTEMFARFTTVAGER
                     GAADAERDIRGFALKFYTEEGNWDMVGNNTPVFFLRDPRKFPDLNKAVKRDPRTNMRS
                     ATNNWDFWTLLPEAFHQVTIVMSERGIPASYRHMHGYGSHTYSFINAANERFWVKFHF
                     HTQQGIKNLTNEEAAAIIANDRESHQRDLYEAIERGDFPKWKMFVQIMPETDADKVPY
                     HPFDLTKVWPKSDYPLIEVGEFELNRNPENFFADVEQSAFAPSNLVPGISVSPDRMLQ
                     ARLFNYADAQRYRLGVNHHQIPVNAPRCPVHSNARDGQGRVDGNYGSTIHYEPNSFGK
                     WQEQAQYAEPPLKINGDAAHWNYREDDADYFSQPRVLFNMMNDEQKQALFNNTAAGMG
                     DALDFIKYRHIRNCYACDPAYGEGVAKALGMTVEDAIAARATDPALGQPGLL"
BASE COUNT         6690 a         4960 c         5189 g         6689 t
ORIGIN      
        1 aatgatttat gaggttttaa atgatcattt catctgcaaa cgattaccgc cgtgcggcaa
       61 aacgtcgtgt tccccctttt atgttccatt atgcggatgg cggttcttac gccgagcaga
      121 ccttacgtcg taatgtgagc gatttggaag acatcgccct gcgtcaacgt gtgttaaaag
      181 atatgtcgca actagacacc ggcatcgagc tgttcggcga aaaactgtct atgccggtga
      241 ccctcgcccc tgtcggtgca ttaggaatgt atgcccgccg tggcgaagtg caagcggcca
      301 aagccgcaga taacaaaggt attccgttta ccctttccac tgtctctatt tgcccgattg
      361 aagaggttgc accggcaatc aaacgcccga tgtggtttca gctctatgtg ttaaaagatc
      421 ggggctttat gaaaaatgca ttggaacgag ccaaagccgc cggttgttcc acgctagtgt
      481 ttaccgtaga tatgccgacc cccggcgcac gttatcgtga tatgcactcc ggaatgagcg
      541 gcccatacaa agacattcgc cgtgtgttgc aggcaatggt tcacccgttt tgggcgtggg
      601 acgtgggcat tcacggtaaa ccccacacgt taggcaatgt gtcaaactat atgggtaaac
      661 cgattgatct gaacaactac atcggctggc tgaccgataa tttcgaccca tcaatttcgt
      721 ggaaagattt agagtggatt cgtgaatttt gggacggccc gatggtgatc aaaggcattc
      781 tcgacccaga agatgctaaa gatgccgtaa gatttggtgc ggacggtatc atcgtctcca
      841 accacggtgg tcgtcagtta gatggggtac tttcttctgc caaagccctg ccaagcattg
      901 ccgatgcagt aaaaggggag atcaaaatcc ttgccgactc tggtatccgc aacggcttag
      961 atgtggtgag aatgctggct ctcggcgcag attgtacgat gatcgggcgt tcctttgttt
     1021 acgctcttag cgccgccggt caagccggtg tggaaaacct gctcgacatc ttccttaaag
     1081 aaatgaaagt ggcgatgaca cttaccagca atgctaaaat tagcgacatc ggcagagatg
     1141 ctttggtaga tttaagtaat ctttagctca aatagccaca atccgcacgt tttaggtgcg
     1201 gattgttatt ttagataggc taatttggca ttttggtaat attaatgcgg taatatttca
     1261 atgtatcttg gttgaaaaag agaaattcat cgttatcttt agttttttcg cccagcacat
     1321 aaatgttatt gcgatgaata gataagacat aatatcgctt attttcaatt tcaagataat
     1381 catacgttgt tctcagctca gggcgaatgt agccaatact caaagaaagt aagctaaaat
     1441 agatgaaaat aaaaatatag aaaaaaccga agcgttcttc gcttaacatt ttttgaatat
     1501 ctaagtggaa tacgcggtgg tcccaatgtt tgtgaaataa taatactgat agggtagttg
     1561 tccctagata gaggtagttg tccctagata gagtaggtgt aaatacattg gcactttgcc
     1621 aataaacaga tagaaactga tcattactgg caagctaaaa atagtaacaa gaacactcac
     1681 ccgtagccag ccaaggtatt taaagtaatg cagcttaaaa actttattaa ctaaagcgta
     1741 tcctatcaaa taaaaaagaa agatagttaa aaatgtgcca aaaacgtagg ctagtgcgcg
     1801 agctataccg gcattaccaa cctcaacatg ccaccacggg tagccatgaa aagcagcaat
     1861 accccaagca taacagtaag cggtagaaaa agagattaaa gtaaagaaag cgagagtaag
     1921 agaagagttg atgaatttct gattagtcat aacagtaaat agattgcacc tacttagtta
     1981 aagatgaaac acaagaaagc agactgagtt tagcttccac ccaactcctt tgtttagagg
     2041 agaactttat taaagcacga cttttgttgt atttcaaacc tttgttttta tacaaaacaa
     2101 ccgtgttgta aaaaatgatc gtaaactgac cgcttgtaat gcactctgaa tatatcgaaa
     2161 catgataaaa atgataaagc atgtttcgat aatttactgt ctttagtctt ttaaatgttt
     2221 tgcatgaaag gctatatgtt caccgataaa gctactaatg aaatagtagc tgtgatcgta
     2281 accatcacgt aaattgaatt ggtagggtat tccaagtcta tctgcggttt cacagaaaat
     2341 ttccggcttt aactgatcgc tcaaaaagct gtcttggctg ccttgatcga ttagaattgg
     2401 caagcttggg cgatattttt ccagcaatgc cacgctatca tacgccttcc aagcggtctg
     2461 atcttcgcct aaatatgcta aaaatgcttt ttgcccccaa ggaacttggg ttggggcgac
     2521 aatcggtgca aatgccgata cactttgata ttgccccgga tttttaaatg caatggttaa
     2581 ggcaccatgt ccgcccattg aatgcccaaa aattgattgt ttaccgttag tcgggaaatg
     2641 agcattaatc aatgttggca gttcgctgac aatgtaatca tacatttggt aatgtgttgt
     2701 ccattcgcct tgcgtagcat ttaggtaaaa tccggcaccc tgacctaaat cataagcctc
     2761 atcattagcg acattcacac ccctagggct ggtatccggt gcgaccacaa tcagatgatg
     2821 ttcgtcggca taacgttgaa agccagattt agtgatgaag ttctgctcgg tacaggttaa
     2881 accagaaagc caatatagta cgggtagctt ttgtgtttta gcagattgcg gcagataaat
     2941 agcaaatttc atttcacagt ttaagctggc tgagaaatgt tgccaaacct gttgttcacc
     3001 gctaaaacag cggtagtttt caaattgttg cataatattc cttacaagcg gtttaattcc
     3061 gctgattttt tgcaaaaata aaggcggaac tcaccgcctt tatattgtaa tatcaacctt
     3121 tcaaacttcc catatcaatc acaaaccgat atttcacgtc tgatttcagc attcgttcat
     3181 aagcatcatt gatgttttgc atatcaatca tttccacatc cggcacaatg ttgtgttcgg
     3241 cgcagaagtc gagcatttct tgagtttcct gaatgccgcc gatgacagag gcggaaatag
     3301 agcggcggcc taaaatcatc ggtacggtgt tgatggtttg ctccaactca ccgaccaagc
     3361 ccaccagcac caatgtgccg tccaacgcta aggttgggat ataaggtttc aagtcgtggg
     3421 tataaggcac ggtatcgatg attaaatcaa aggtatttgc aacgtctgcc atttgctttt
     3481 catcggtaga aagcaccaca cggcttgcgc ccaaacggta agcatcatcg gctttaccca
     3541 gtgagcgagt aaaaagcgta acttccgctc ccattgcttt ggcaagttta accgccatat
     3601 gccccagtcc gccaagcccc acaacagcga cttttgagcc agctttgaca ttccaatgac
     3661 gcaggggcga ataggtggta atgcctgcac acaaaagagg ggcaaccgct ttggtgtcca
     3721 agttttcaga gacttttaat acaaaatctt cactcaccac cacgcatgtg ctgtaaccgc
     3781 cttgtgttgg tgtgccgtca aagcggtcaa taccgccata agtgccgatg ttgcccactt
     3841 cgcagtactg ctccaaaccg tgctggcaag gactgcattc acggcaggaa tccaccatac
     3901 aaccgacacc gaccaaatcg ccgatttgga attttttggc gtttttaccg actttgataa
     3961 tacgaccgat gatttcgtgc cctggtacaa gcggatagct ttgcgtccag ccccaatcat
     4021 tgcgagccat atgcaagtct gaatggcaca cgccacagta caaaatatca atcaaaacat
     4081 cgtcttcaag caattcacgg cgttcaaaac tgtgtggggc aagtggtgtg gttgccgaaa
     4141 aagtggcgta ggatttgata gggtaagcca tttttggggt ttaaatggct gaaatggcaa
     4201 ttactatagt gccgatgatt tattattgaa aatcaagatt tttgataaat cggtatcagt
     4261 taaaatgata attgggggtt ttcagactga ctgaaaaatt attttgccca ttttaaaaac
     4321 aaaaacaccg ccctcatcac aaaagcggtg tttttcatca aaattgtgtc aaagcatact
     4381 ctgccaattc tgtaagcact tcatcagcag gacgctgagt gggttttaaa ttgagtgcca
     4441 aatgtttcac gcctttttct tgttcttttg cccaaaagtc tgccaagcct tttgcaccac
     4501 cacgcagata aatgttgtta aataactgtg ctttggcgtt tttgtcgtcc aaaacttcca
     4561 caaattgagc atagccaaat gctgtggggg tgtctttttg gcgtaaatcg gcaagggtgt
     4621 tgatgatgtt tgcggtgtcg gcaggattca caccgtgcca aatccacgca tcaggtacat
     4681 tagcaagcca gtccatttct tgccttgctc gtccaatcgc cacaatgggc aagcggtgtt
     4741 tgggtttggg gagcaaatcc aaattgtctg ttaaatcggc ataatgtttg cctttgaaat
     4801 ggggaaaatc ctgctcggtt aaggtgttaa tcaaatcaat gctttcacga aaacgctcgc
     4861 tacgagaggc aaaatccgcc ccaaatgcag ggtattccac aggtctatcg ccagaggata
     4921 agcccaataa aaaccgtcca tgactcaaac tatccacact tgtcgccatt ttggcaatgt
     4981 gaatgggact acgcaaaggg gcaatcagcc ctgccgtgcc aagggcgatg ttttgggtgt
     5041 gggcggacaa aaaaccaagt gtaaccatcg ggtcaagccc ttgccccaca tcgccaaaat
     5101 gagggtcata aaacggcaca tctcgtaccc acaatgctga aaagcccaag ttttcagcca
     5161 gccgtgcatt gtcggcaatg gtggtcaaat tgtctggaat gtgattttga tagcccataa
     5221 agggggcaat taagcccagt gttaattgat tttgcccaaa aacttgggag aaaatggggg
     5281 aaagcgtgtt cattttggcg tacctgtctt atttaagata aggtatttta agggcttttg
     5341 agtttgctta aaatgggata aattgagaaa gtattttgct aaattggcat aaatagacgc
     5401 tttcaagctg actgaaaatt tgcaaaattt ttaataaaaa tgaccgcttg cctttcaaca
     5461 agcggtcatt cccctttaat tttttaccac cgtaaacacc ttatttttca gccgatattc
     5521 acttttttca tcgtgagcca gttgcacaat ggttggcttg cgtttacaca attcacgcat
     5581 tgcacacgcc gtcaatgttg ccagcaagca tatcttgttc agagtaagag gcatcgccgc
     5641 caatcaaaat ataatgctcg cccatatcca ccaccaccgc caaatgcccc agcgtatgtc
     5701 ctgcggttgg cacaaggcgg attttgccgt ctttggtgag tttatgactg ctctcaaaac
     5761 tttcaaacgc cccatcatta aaatagatgc catcaggctt aaaccaatca ggatagtgca
     5821 ttgttaaata accgttcatc accgcattgg gggcgttgat tgcctgtttt tcagtctcac
     5881 tcatcacaaa tcagctgttt gggaaattgg gcaatccgcc tgcgtggtcg ccgtgcatat
     5941 gggtcattac caccgtgtca atgcgtaaag ggtcaaagcc gtttgccttt aaaatttcgc
     6001 ccacttcttc gtgttttttg accccacgcc gttcgcaaaa ctgcataaac gggtgccacc
     6061 acggctgata gcccttgtcg ttggcacgac tgctttcgcc cgtatccacc aaaatcaaac
     6121 cgtcatcgtg ttcaatcagc cagcaaccaa tcggcagttt aggcgaccat tttttatcgg
     6181 tcagcacatc aagcacacgg gcaggacggg caaaaaagcg tgccacttga tgatgaattt
     6241 tgatttgtac ccaacctgtt gtcagacaat gcactttaat catttttgtg ttctcattaa
     6301 aaaatcgtgg gcatattgta gaaagtgcgg taacttttca caaacattaa aaagttcgta
     6361 tttggctgga tatgcagata aaattacaaa aatgtatgct tattttgaaa aaagatgaac
     6421 gatttaagag taatcaaaac ccatctagca atccaaaacg ctttgattga attattagat
     6481 gaaaagctgt ttgaaaaagt gcaagtccaa gaaatcattg aaaaagcctt gattaaccgc
     6541 accacttttt accgttatta ttcgggcgaa agtgatttgg tcggcaaatt gataaaagag
     6601 gtgaaggcgg aatatcaaga cctgctcaaa aagcgttttg aaagcggtaa tttgctgcga
     6661 tttatgcaaa ccattggcaa ccgcttattg gaaaaaagac ggctgatttt ggcactttgg
     6721 caaatcaaaa cccctcgcca tcatttgtat gacgatatgc acatgctgtt aaaaaacgcc
     6781 tttatcgccc acgccaaacg gataacatca gatgataaaa actgggctga gaaaaattgg
     6841 gattaccaag cccatatttt tgccaccatt gcccttgaaa gccataaata ttattttgaa
     6901 caaggcaaac ttttgcccat tccacaggtg tttgaggagt ggatagaaat ggtgcaggtg
     6961 ttgaaaagtg tttagaaata actggtaaaa ttttccataa aaaacaccgc tttcatcaca
     7021 aaagcggtgc tttcaaatca tcaaacctta accacaatct taccaatctg ctgattgctt
     7081 gccatatatt cgtgagcttt aaccatttca tcaaaggcaa agattttgtc aatttcaggg
     7141 cgtaactgac cgcttgccaa accgtcatag acaaaggttt tggcttgtgc caatttttca
     7201 ggtacggtgg taatttcaaa gagttcatac ccccgcacgg tcaaatgctt acccaaaatc
     7261 gggaacactg gcacagcaat atcatcatgg cttaacgctc cataaatgat gtaacgacca
     7321 tcttgtgcca ttgcattgat gatttttgcc gcctcttttc tgcccacagg gtcaaatacc
     7381 acattgacgc ctttgccatt ggtgatttca aaaagtttgg cggttacatc gtcttcgctt
     7441 gtggcaatca caaaatccgc tcctttttcc aaaagtacat tgcctttggc gtgggtgcga
     7501 gacaaggcaa tcacggttgc cccttgcatt ttggcgattt ggatactggc taaaccaacc
     7561 gaactggttg caccgcccag caccacaaaa tcgccttttt gtactttgcc aaactcaatc
     7621 agtccgccat aagcggtaac aaacgccatc caactggcgg acgcttgttc cattgacaaa
     7681 ttatcagggt gcttgaccac agcgtgtttt ggcatattga cgatttcgcc ataagtgccg
     7741 tattcggtaa acataaagga cggaataatg ctcaccttat cgctaatggc aacttcgttc
     7801 acaccctcgc caatcgccac aatcacacca gccccttcat aacccaaagt cgcagggaat
     7861 acagggtcaa tcacatacgc cccttcacgg tacatcattt cggcacggtt taagccaatg
     7921 gcttggattt ggatttgtac ttcgtgtgct tttggtgcag gaattgctac atcaacgatt
     7981 tgcaagacat caggcgaacc tgtttttgta aattggattt gtttgctcat tgttttgacc
     8041 ttttgtgtaa ataatgggcg tattttaatg caatgaattt attttaaaca caacatcaat
     8101 tgcaaaacac ttttgctaaa tttacataaa tacattttca caatgtctga aaagttgttt
     8161 tgcaaatttt aaaaagaaaa ccaccgcttg ccgatcacaa gcggtggttt ttgtgggtta
     8221 ttttgcaaga ggtgtcagct tggctacagt aagcggataa atgcgacgcc ggctaagagt
     8281 aaggtaacgc cgatgatctg cagcggtaga atgggttttt gtgtaatgcc gaacagaccg
     8341 aatttatcta ccagcaggct gccgcaaatc aggccagaga ggacgagcat caccaccgag
     8401 cctgtgccga tttgggggac gagccaaacg ccagcgagga taaacaggct gcccagtatg
     8461 ccgccgctcc atatccaaaa cggtttgcct tcggttggct ggcagagttt cggcaggctt
     8521 ttttcataaa acagcactac cagtaataag ccgatggcac cgacaccgaa ggagactgtt
     8581 gctgcgccga gtgatgagcc gggctgtctg ccgaattcgc cattaacggc ggcttgggtt
     8641 gctaatgcgg ttccgcctaa tatgccgact gcgcgccaca gccataaatt tgcgttttct
     8701 ttgcgtttta gaccggacag atggggcagt accacggcaa tccatacgcc gaataagact
     8761 aaaccaatgc caaacaggcg gctggccgtg aaggctatgt aaggcgagtt aagccaagcg
     8821 aaagtgtcga tcagcacgcc catcagcact tggcctaaaa tcggcatgac cgcagtttgt
     8881 acgccgccga gcttggggaa gatcagtacg tttgccgtta gccccaccat gccgagtaat
     8941 ccgcccgtcc atgcccataa ggggacgttg cctaaattgt cgaggctaaa tgctggtgtt
     9001 tgttgcatga tgagcgagag tccgagtaga aacaggaggc ctacgctgaa agagacaaac
     9061 gaggttaaaa atgccgcacc cacgtgcata cgcaaacggg cgttgatggc tgattgaaat
     9121 gcaatcagca cgccggctaa aataccgata ataaatgtca tgactaactg ggttgtgctg
     9181 ccgcctcaaa atgagtggtg gttgaaaaga aatgccgtct gaatgcggat tcagacggcc
     9241 ttaaggcttg gtatcggcgt ttacctgatg ttgaatagcc gttttgccta gccggtttat
     9301 tcgatgaatt tcaccacggt ttcgaaatct ttacgtgctt tttggcggat ttctttatca
     9361 cggtaaccga aggtacacat caccgatacg ccccattcgc ttgggtcaaa taatccttcc
     9421 gctgccaaaa tatcgttgac ttcttggtaa gcgaaacctt ctatcggaca agaatctacg
     9481 ccaattaaag ctgcaccggt catcatattg cccaatgcga tgtaggtttg tttgcttgcc
     9541 cagtcaaaca ggctgcgttc gctgtctaaa atgccgatat ctttctcttg gaatttttta
     9601 tacacggcca tggctttttc gcgcgcttcg ccttgtaaac ctcgtctatc cataatttca
     9661 gaaaaatatt ggctgtcgta acgggcattt ttcttcgcta aaatcaccac gatatggctt
     9721 gaggtttcca tagttggaat gccccagcag taaggtttga ttttttgccg taattcaggg
     9781 ttttgcaaca ctaaaaactg ccaaggctct gagcctaccg aacttggact taaacgaccg
     9841 agttccagaa tatagttgaa atcttcagcc ggaatttttc tgctgccgtc gtaagaacgg
     9901 gtagatgcac ggtaatgaaa ggcattgatt acgtcttctt tgttgaaaaa ttgcatggaa
     9961 cactcctaaa aatgcaaaac agtcttttca gacggtctga aaagtctaga tgaaatacgg
    10021 ataagtataa aagatttaag ttatttttaa taattgataa ttttgatatt taggtataag
    10081 ttgacgtgat gagaaaaaac agaccctctg aatgttcagg cggtctttaa cgttggttgt
    10141 ttacataaat aatccgcgtt catattgctt cggtacaaac tgcatttcac tgctgtctgc
    10201 tgcattttgg tcatattgcg cattcaatac ccagtacgga tcgcgcaata aggctcttcc
    10261 cacagccact aaatccgcat cgcccatacc taagatatga tcgcctaccg ccggattatc
    10321 caacatacct actgcaatta ccggtttgcc cgtagcgagc ttcacttgac gggcaaagtc
    10381 tacttggtaa cctgcgtaaa attcggggtg attacctgca tgcaatacac catcaccacc
    10441 accgctgatg tctaagacat ctgcgcctgc atcggcaaaa cgtttcgcta cctgcaaacc
    10501 ataatccaag ccaaagccgt tttcaccgta ttcttgtgca gaaatgcgga caattaacgg
    10561 catttcagac ggcatcactt cacgtgctgc ctgaatcact tgctcgccaa acaacatttt
    10621 gtcttggccg tactcatcac tgcgctgatt ggatttcggc gaatagaatt ggtgaattag
    10681 ataaccgtgt gcgccgtgga tttcaattgt atcaaagcct gcctcaacgg cacgtttgac
    10741 cgaatcctta aaggcttgaa ccaatgcttt gacttcgtct gtcgtcagct cgcgaggtgt
    10801 aatcagattt tgtccggcat aatccaactc accgtagtga attgcacttg gagccactgc
    10861 atccggctcg tcttgggctt tgcgtccggc atgggcgatt tgcacaccga tttttgcccc
    10921 ttgagcatga acgctgtcta ccagtttttt gaatgcctgt tgttgttcat cgttccataa
    10981 gcctaagcag tttggcgtaa ttctgccgtt tggcgccaca ttggtcatct ccacaataat
    11041 caagccgaca ccacctactg cacggctggt gtaatgcacg aaatgccaat cgttcggtac
    11101 gccgttggtt gccgaatatt gacacattgg tggcataacc acacggtttt tcaatgctaa
    11161 gtttttaatg ttaaacgggg tgaataattt tctgaacttc gccatgttgc cttccttaat
    11221 gaaatagatt aggtggaatg ataattcagt gctattatct attcaaattg attattgtga
    11281 tagttaggta taaaaatgag tgatataaga acactggatt tcaatttgct caaagctttt
    11341 gtggtgctgc tggacgaatg caacgttagc cgggcagcac aacgattatc catcacccaa
    11401 ccggcaatga gcggtatttt aaaccgcttg cgcgagagtt ttaacgatcc gttatttgtg
    11461 cgagtgcaac acggtatgca gccaaccgac cgcgcgttgc aactggggca aaccgcacgc
    11521 aacattctgc aagatatcag ctccatgctg caaccaccca tattggaacc ggaacatctg
    11581 aatatgacct tgcgtattgc cgcaatggat tatgtacaac aaattattgc cttgccgctg
    11641 attctgcgtt taagacggct tgctccgaat gtaaaagtcg ccttactgcc ggtgcagggg
    11701 caaaatatca aaactttgtt cgagcaaaaa aaaatcgatc ttgccttggt cagccgccaa
    11761 catttaagca cggatacgct gaaaacggta ttatatgaag aacgctacgt atgcgcgatg
    11821 agcaaaacac acccgatggc gcaaacaccg ctatcgctcg atcaattctg caatcttccc
    11881 ttcgcaatgc tttcttacaa cggcggagaa ttcagcggag ctacggatct agcgttacaa
    11941 aagataggac tacagcgcaa agtaatggtc tcggtaaacc atatttccct gctgccccag
    12001 ctgctacaag gttccgactt agtcgcagta ttgcccgaac gcttagcaaa aaccttaccc
    12061 aatgtttatt tacaacctcc gcctatagag gtagaaggct tcaccatgat gatggcttgg
    12121 cacgaaagaa ccgaacaaga tacggcgcat cgatggttaa gagaggtgct gcagggtgtg
    12181 gtaaaaccta aacaggctct ttaattattc tttcttttgc tatgaaaaaa gccgtttata
    12241 tttttagata aacggctttt ttgcattcta atattagtat ttttctacaa actgagcata
    12301 ggcattaatg aagttttgca ttgagctttt taatgtctca gaggcaattt caccctgttc
    12361 atcaaatacg cctgtaaata agtttcctac gtttaatgcg aaaggtaagg ttggcatatt
    12421 tacaaatgag cctgaagtaa tagtacgtac ttgatcagct gcacgtacac cgcctgccgg
    12481 gcttgcgcca acggttacga tacctgccgg tttgccaatc catttgcttt gccccattgg
    12541 gcgagaaacc acgtcaatcg catttttaag cattgcagaa aagcttgcat tatgctcagg
    12601 tgtgactaat aacacgccat ctgccacagc aaccgcttca cgcactctgg tgtattgtgc
    12661 cgggctgttt tcatctaaat cacggtcgta aagtggtaag tctgcaatat ccacaatgtt
    12721 taattgaatg cttgcaggtg ccattttttg taagtgacga acagtcaagt gactaaatga
    12781 agttttgctg ccgctaccaa ttaatactgc aatttgtttt gccattttat aattccttag
    12841 tacatagaga atataggcat tttatccctt tcaatctgtt tcataaagcg cttatgatga
    12901 aaagcactat ttacttatat taaataattt tgcaaatttt aggccaaatt tgaccgcttt
    12961 tatctttagc taaaaaggtt ataatttgta gaaatagatc ttaatttagg gtttttatgc
    13021 tttttctaaa caaaataaaa cagcttttca tcttcgcctt tacgctagtg ttatctgctt
    13081 gcagtttggt tgtttataag ccagttgaaa caattaaaca ggtaaatgtt gaaagaggtt
    13141 atcgacaaaa aaatgtgatc gctgaaagtg caaaagacgg taatattgtt gtcatcatgt
    13201 tttcaggtgg tggaacaaga gcggcggcgc ttggttatgg agtgttggaa gagtttaaaa
    13261 aggtaagggt gaaagaaaca aataaaggta atactttatt agagaatata gatattgcct
    13321 ttggcgtatc aggtggatcg atattagcca cttattttgc gttggaagga aaggatatca
    13381 ttcctaaatt tgctgaccgt tttttaaaag cgaactttca aaaagatatt attacagagg
    13441 tgttttcgtt atccaatctt cctcgtttaa gttctcctca atatgggcgt ggtgatttat
    13501 tgcaagaacg cttaaatttt gccctttatc atggtaaaaa gtttggtgat ttagtgaaaa
    13561 atcgtaaagg accttttgct gtgattagcg caacagacat gaacattggt caaaaactga
    13621 cctttacgca agaaaccttt gatgggcttt gtttaaattt gaatgattta gaaatttccc
    13681 gtgctgttgc tgcttctagt tctgttcctc tgatttttgc gccgcttaca ctgaataaca
    13741 atggtggaaa ttgccatttt agtatgccac aagaatttag tgctagagaa aaagcaattg
    13801 atggactaat tacaaaaaat attgaagaaa cgaatcatat actcagcctt tatcaagata
    13861 gtaaagaacg cccctttatc catttagttg atggtggatt aacagataat ttagggcttg
    13921 ccagtgtgct ggaaatatct gatgttatgg gtattgaaga gatgtaccag tatattcatc
    13981 agcttagggt gaaaaatatt gtagtgatta atgtaaatgc gcaaaatgaa gtatctagcg
    14041 agatcgataa gaccgcaaat gtaccggggg tggctgatgt agtgaataca atagttaatg
    14101 tgccaattga tagaacgaca caaattaatt tacaacgtta tcgccagttt acagatggtt
    14161 ggaatgaata tgcaaataaa cagccatcaa agatcgaaat gcattttgtc agtttaggtt
    14221 taaaagaatt acctgatggt gatttaaaaa aagatgtgct aaatattgca acatcatttt
    14281 atttgccgga agaggatgta gataaattac gtgaagctgc tcgggtttta ttgcagcaat
    14341 ctcaagaata taatgcggca ttaaaaacac tgcagtagta aaaaaccgac caagcggtcg
    14401 gtttttggta tttttttgca aatatttatt cgctgatgtc gtttaaatcc aacaactctt
    14461 gcgaaagaat aatgccggtt aagtcagcat aaacgtaatc ttcggggaag aaggttacac
    14521 cgccaaaatt cacaggcgta tccacttcgc caatgtcggt attatctgct cccactggaa
    14581 ttggcgctaa agcatgaata ccgatatcta atctttccaa aatatctaat tggcgaaccg
    14641 caccataaac gatgatacct tcccagccgt tatctacagc aagttgagcc agctctgcgt
    14701 caattaaggc acgacgaacc gcaccgccac catcgactaa cagcactcgc ccttcaccct
    14761 cttcttccaa aacttcagca attaaaccgt tgctttcaaa gcatttaacc gtggtaatct
    14821 tcccaaagaa ggaagaagca ccgccaaagc ttgagaaaat tggttcaacc acatcaactt
    14881 ggtcagaata aagatcgcaa agtgcagagg tatcaatacg cataatatgc tcctgttcgt
    14941 agattattta gctaaagtat aaaccttcgc ccaaaatttg caaaatttta tcgaaaaaag
    15001 accgcttaca ggcttttttc ttatgcttta ttttctagtg aaccgctttt aatatgcaat
    15061 tccatttggt tgtaaggaat agatatacct gcttcaccaa aggctaattt tacttgttcg
    15121 ataatttccc aatatgctgc ccagtaattt ggtgtctgaa cccaagggcg aacaacaagt
    15181 tgaacactat gcggtgctaa agcgccgact gctactacag gtgccggatc ttttaataca
    15241 taagtattgg cgtctaaaat gcgttggata atctctttcg cctcttttaa gttagattca
    15301 taagaaatat caaaggtgaa atcaatacgg cgtgtcgggt tgttagagta gttgatgatg
    15361 ctgtcagaaa acactttgcc gtttggtaat aacacggttt tgttatcgcc ggtgcgaagt
    15421 tccaacacta aaagccccat ttgttcaacc acgccggtca ttccgccggt ttcgattaaa
    15481 tcccctttgc gaaatggctt gaagattaac aacatcacgc cggcagcaaa gttttgtaat
    15541 gagttttgta atgataaacc gatggctaaa ccggcagcac cgattaatgc gaccagcgag
    15601 ctggtgttaa tgccgatttg tgaaagcgag gcaatcacca caattaacag cagtaaaaaa
    15661 tagctgattg agacaataaa actttgcagc atttcatctt tggttgattt taatgcggct
    15721 ttaccgagta aacggctaat taggcgggca agaaatttcc cgactacaaa aatcgcaatc
    15781 gccagtaaaa ttttggtgcc ataagggata atccactcat ttagcatagt ttcaatattc
    15841 atattactga ctttgtggat tactttttcg gtttcggcaa ccacgtcaat gtttggagct
    15901 gtttcggtga ctttttcagt catattgttt ccttaaaatt aaaatgaaaa ataagcctag
    15961 cgaatttgcc tcactcgcta agcggttttg tgctagaatt tagagcaaaa ttttctctaa
    16021 aagacaagat aatgacaaca gaaaaagtaa ttagtgccgg cgttcaattt tttacgatca
    16081 gtgaagatga agccggacag cgtttagata attttttatt ggcaaagctc aaaggcgtgc
    16141 cgaaaagttt gatttaccgc attgtgcgaa aaggcgaggt gcgggtgaat aaaggacgaa
    16201 acaaacccga atataaatta cagcctgatg atttagtgcg tgtgccgccg gtgcgtgtgg
    16261 cggaaaaaaa tgaagcccca atttcgacta aattaaacaa agtggcggag ctggagagcc
    16321 aaattctgta tgaagatgag tcaatgctag taattaacaa accttcaggc attgcagtac
    16381 acggtggcag tggtttaagt tttggtgtga tcgaagcctt gcgtgcatta cgtccacagg
    16441 ctcgtttctt ggaattggta caccgtatag accgtgatac atcaggcatt ttgctggtgg
    16501 cgaaaaagcg ttcggcgtta cgttccttgc acgaacaact gcgtgaaaaa gtagtacaaa
    16561 aagactatct tgcccttgtg cgagggcaat ggcaggctca taccaaagtg attaaagctc
    16621 cattgctaaa aaacgaactt gccagcggtg agcgaattgt acgggtgagt gaagagggta
    16681 aaccgtccga aacccgtttc agcattgaag agcgttatgc caatgccact ttggtgaaag
    16741 cttcgcctat taccggaaga acccaccaaa tccgtgtgca tacccaatat gccggacacc
    16801 cgattgcgtt agatgacaaa tatggtgatg cggaatttga cagcaaaatg aaagaagtgg
    16861 gcttacagcg tttatttctg cacgcccatg ctattcgttt tgaacacccc aaaaccggcg
    16921 aagaaatggt cattaccgcc ccgctggata aaactttaaa aggcgtattg gcaaaattaa
    16981 gggctaatta ctaaataact taatgagaga gtaagatagc gcaagcgtcc acgcttgtgc
    17041 tgtaaatcaa catagagtgg tacaagcgtg gacgcttgca ccaacaaaat ataagcaaaa
    17101 atatgcaaat ccatttcaaa aattttcaaa aaaatgaccg cttgttagat ttaaccacac
    17161 cgcaaattat ggggatctta aacttcacgc cggattcatt ttccgattcg ggcaagtttt
    17221 tttccctcga taaagccttg ttccaagcgg aaaaaatgtt agctgatggg gcaagcatta
    17281 ttgatattgg cggtgaatcc acccgcccta atgcggcgat tgtgagctta gaacaagaat
    17341 tagagcgtgt tgtgccggtg gtggaggcga tccgcagccg ttttgattgc ttgatttcgg
    17401 ttgatagctc caaagcagaa gtattcaaag agtcggcaaa agtcggtatg gatatcatta
    17461 acgatattcg agccttaacc gagccgaatg cgttggcagc ggcagtagaa ttgggcttgc
    17521 cggtttgttt aatgcatatg caaggcaacc cgcaaacaat gcaacaaaat ccgcactatg
    17581 atgatgtgtt ggaagatgtg gcagatttct taaatcaacg gattttcgag tgcatcaccg
    17641 caggcattcc caaagaacac attattttag accccggttt tggctttggt aaaagcgtga
    17701 aacacaatta ccaattattg aaacacctta aagcctttgt ggattcaggc ttccctgtgc
    17761 ttgccggact ttcccgtaaa tcaatgattg gtgcggtgtt agataaaccg gtagaacaac
    17821 ggctacttgg ctctgtagta ggggcgttat tagccgctca aaatggggcg aaaattttac
    17881 gggtgcacga tgtggcagaa accgccgatg cgttaaaaat ctggcaggca atgctggaat
    17941 cagataatga ggaaaaatga tgacaacctt agtttatggc attaaaaatt gcgacacagt
    18001 aaaaaaagcc ttgaaatggc tcgaagatca taatcaaaac ccacaactgc acgattaccg
    18061 tgtggacggt ttaaatgccg attggttagt ggaaatggaa gcaaaattcg gttgggaaaa
    18121 cttagttaat aaacgtagca ccacttggcg aaaccttgat gaggaaatta aaaacaacct
    18181 taaccgtgag tcggcattaa aagtgctaac cgaacagccg accctaatta aacgcccgat
    18241 tgtgattaaa gaggatattg ctttgattgg ttttaatgag aaagagtatg tggctgcgtt
    18301 taattaattt tttcccctgc ttgtagggac aggtcctgtg cctgtccgta gtctttagga
    18361 ttgttcgggc gggcacagga cccgccccta caggatatat agcaaaaaat tcacccaaaa
    18421 caaccgcttg taacctatga aaaatgaaat tatttccctt gcacagaacc taatccgccg
    18481 tgattccatc agcccacaag acaaaggctg tcagcaagaa attgcacaac gtcttgaaaa
    18541 actgggcttt aatattgaat ggttgccgtt tggcgacacc ttgaatttat gggcaacgca
    18601 cggctcggaa aatggtaaaa cagagccttg cattgtgttt gccgggcata cagatgtggt
    18661 gccggtaggc gatgaaagcc aatgggctta tccgccgttt tcggctgaga ttgtagatgg
    18721 aatgctttat gggcgagggg cggcagatat gaaaggctcg ctctcggcgt tagttgtggc
    18781 ggcagaaact ttcgtgcaac atcacccaaa ccataagggc aaagtggcat tattgattac
    18841 ctccgatgaa gaggcagcag ccaaagacgg tacggtaaaa gtggttgaaa ccttaatggc
    18901 tcgcaatgaa gcggtacatt atgcggtagt gggcgaaccg tctagtagca aggtgtttgg
    18961 cgatgtgatt aaaaacggca gacgtggttc aattacggca gatctctata tcgagggcat
    19021 tcaagggcac gttgcctatc cacatttggc ggaaaatccg gtgcatacgt cactgggttt
    19081 tttaaccgag ctgaccactt atcagtggga taacggtaac gaatttttcc cgcctaccag
    19141 tttgcaaatt gccaatatca aagccggcac aggcagtaat aatgtgattc cgggcgagct
    19201 ttatgtgcag tttaacttac gttattgcac tgaagtgacc gatgaaatca tcaaagccaa
    19261 agtagcggaa atgctcgaaa aacacggttt gaaataccga ataagttgga atttatcagg
    19321 caaaccattc cttgccggca atggaaaatt ggtcgaagcc gccttacaag cggtcgaaaa
    19381 tgtcacaaaa attacgcctc gtttagacac aggtggcggt acttcggacg gtcgttttat
    19441 tgcattaatg ggggcagagg tagtggaatt tggaccactg aacgccacta ttcacaaagt
    19501 gaatgagtgt gtgagtgcgg atgatttggc acattgtggc gaaatttatt atcaaatttt
    19561 agaaaaatta ttggcaactg cataacccta aataggcaga atgttatcaa attgtattct
    19621 gcctattttt tatgtagcta ttctgttaaa cagcgtttac actagcggca gtttttatat
    19681 ttataggtaa atgttatgca actctactct tatatctgct tgctgtttgg gcttgcgatg
    19741 tttatttcgt tttttacccg aaaattaaac gaaaatattc aaaccactat tgcaattaca
    19801 gcctctgccc ttgtcggctc ccttcttatt cttaccttcg gttctctcgg ctggtttaat
    19861 gttgataaac ttgccatttc catttttgaa caactcgatt ttaaaagctt cctgttaaac
    19921 ggaatgctgg ggttcttgct ttttgccggt tcacttggga ttaaactacc attaatgaaa
    19981 gagcaacggc gggaaattgc agtttatgcc ttattttcaa ccttagtttc tactttcttg
    20041 atcggtgcgt taatttatgg cgtggcaagg ctacttgggt tagagctcgg ctttgtgtat
    20101 tgtttgctgt ttggctcact gatttcgcca accgacccga ttgccgtatt ggcaattatc
    20161 aaaagcctga aagccccaaa acgcctttcg atgcacgtgg agggcgaatc tctgtttaat
    20221 gacggtatcg gcttggtgat ttttaccact atctttgccg ttgcttttgg cggacaagaa
    20281 ccaaacctta gcggtattac cggactgttc ttgcaagaag cggtaggcgg tatcgttttt
    20341 ggcttattaa ccggttttgt ggcacataaa tttatttccg caaccgatga cggaagcctt
    20401 gaaattttaa ttacgctaac gattccaaca gtcggctttg tgattgcaaa ctacctacac
    20461 gtttcagggg cattaacaat ggtagttgcc ggtattatga ttggcaactg gactcgccgt
    20521 tccggtttct ccgaacagag ccaaaaatat cttgatcact tctgggagat gatcgaccat
    20581 tcattaaact acctgttatt cctgcttatc ggcttaagta cactgttagt tgatttcacc
    20641 tttattggtg gggtgttaat gttggcagca attccaattt gtttgcttgc acgctatatc
    20701 agcctgtgga ttccatacca agtgttaaaa cgtttccgta cttataaccc gtacacctta
    20761 aaaattatga cttggggcgg tttacgtggt ggattagccc ttgcgatggc actttccgta
    20821 ccggcagggg ctgcggtggt atcgagctca gaaggcaatc ttgatgtacg tgatttgatt
    20881 ttgttaatga cttatgcggt agtcactttc tcgattttag tacaaggttc aacggttgaa
    20941 ccgatgataa aaaaatccaa gttggtagat ccaagaaaag tggcactcaa taaattaggt
    21001 gcacctgatg atgtaattcc acatatttaa tagcatttat aactgaataa catatcattt
    21061 tgctaaagat tattagccat ctagacggct ttatatttaa atacctctca cgttaatttg
    21121 ttgagaggta ttttttatgt tattcacagg attattatgt ggctttctac tcgggtttgt
    21181 gatgcaaaga gggcgttttt gtattaccgg agcatttcgt gatctctatg taacaaagaa
    21241 tagccgtatg tttgtggcac ttttaattgc aattacggtg caatcgatcg ggatttggct
    21301 tttgtatgaa gccggctctt ttagttctcc ggcggaagat ttgcctttgc tcgcagtgat
    21361 cattggggca tttctgtttg gtatcggcat tatttatgca ggcggttgtg ctacaggaac
    21421 gtggtatcgt gccggtgagg gtttaatcgg cagttgggtt gcactggtta tttatgggct
    21481 gttttctgct tctatgcgaa ccggtgtgtt agccccactt aatcaagagc taaaaagcaa
    21541 tgtaattcaa caccgcacga tttatgaaac atttggtatt tccccttggg tgttagtgtt
    21601 tatcctgagt gtaattacat tggcattatc cgcccacagc atttaaaagg ttaatttttt
    21661 aaggagtaat aaagatgaac gtacgtttac ctgcctcagg gcttgtttgc ccgtttccat
    21721 tagtcgaagc caaagcggcg atggcaaaat tgaataaagg cgatagctta acgattgaat
    21781 ttgactgtac tcaagcaacc gaagccattc caaattgggc ggaagaggaa ggctatgaag
    21841 ttaccgatta ccaacaactt ggtgatgcct tgtgggaaat tacggtggtt aagtaattag
    21901 caataaaaaa gctctacttt tttgtagagc ttttgctttg agttttgtat ttactaagga
    21961 ttagagtaaa cccggttgac ctaatgctgg gtctgttgct cttgcagcaa tagcatcttc
    22021 aaccgtcata cctaatgctt ttgccacacc ttcaccatat gccggatcac aggcatagca
    22081 gttacgaata tgacggtatt taatgaagtc taatgcatcg cccatacctg cagcagtatt
    22141 attgaataat gcttgttttt gttcatcatt catcatattg aacaatacac gtggttggct
    22201 gaagtagtca gcatcgtctt cacgatagtt ccagtgggct gcatcaccat taattttcag
    22261 tggtggctct gcatattgag cttgttcttg ccatttgccg aagctgtttg gttcgtagtg
    22321 aatggtacta ccgtagttac cgtccacacg accttgaccg tcacgagcgt tgctatgaac
    22381 cgggcaacgt ggagcgttta cagggatttg gtggtggttc acacctaaac ggtagcgttg
    22441 agcatccgca tagttgaata aacgagcttg taacatacgg tctggagaca cgctgatgcc
    22501 cggcactaag ttacttggtg cgaatgcaga ttgttccaca tcagcaaaga agttttcagg
    22561 gttacgattt aattcaaact cacccacttc aattaacgga tagtctgatt tcggccatac
    22621 cttagttaaa tcgaacgggt ggtatggtac tttgtctgca tcagtttcag gcataatttg
    22681 aacaaacatt ttccatttcg ggaaatcgcc acgctcaatc gcttcgtata aatcacgttg
    22741 gtgactttca cgatcgttag cgataatagc ggctgcttct tcgttagtta agtttttaat
    22801 gccttgttgt gtatggaaat ggaatttcac ccagaaacgc tcattagctg cattgatgaa
    22861 gctataagta tgtgagccat aaccgtgcat atgacgatat gaagccggaa taccacgttc
    22921 actcattaca atagtcactt ggtgaaatgc ttctggtaat aatgtccaga aatcccagtt
    22981 gttagttgca gaacgcatat tcgtgcgtgg gtcacgttta accgctttgt ttaaatccgg
    23041 gaatttacgt gggtcacgta agaagaacac aggggtgttg ttacccacca tatcccagtt
    23101 accttcttcg gtatagaatt ttaatgcgaa accacgaata tcacgctcag catctgccgc
    23161 accacgttca cctgcaacgg tggtgaaacg agcaaacatt tcagtttttt taccgacttg
    23221 gctaaaaatt gccgcacgag tgtatttagt gatgtcgtta gttacagtga aagtaccgaa
    23281 cgcaccggaa cctttagcgt gcatacgacg ctcaggaatg acttcacgca cgaagttcgc
    23341 taatttttca tttaaccata aatcttgcgc taataatgga ccacgtggac ccgctgttaa
    23401 gctgttttgg ttatcaacaa caggtgcacc gttgttcatt gtcaaatgtg taacaggaca
    23461 tttagacata atagaatcct cataaattag aaataagata ggtaattaat ccgaagagaa
    23521 ttataggt
//
LOCUS       DANLYI010000013        22008 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.013, whole genome shotgun sequence.
ACCESSION   DANLYI010000013 DANLYI010000000
VERSION     DANLYI010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 22008)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22008)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22008
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.013"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            36..1058
                     /locus_tag="PXD81_000416"
     CDS             36..1058
                     /locus_tag="PXD81_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL5536484.1"
                     /translation="MANGTSRNRRSSGRVTLADVAKVVGVGQMTVSRALRTPEMVSEP
                     LRKRIEEAIIELGYIPHFSARQLASAESHNLVIVTTSISSTENTLILSALRKSVADSA
                     LQMVILIADQESWFQELINHAPQAIVFLNLDCPPNVREWIKNSNIPTVEIGAIKKEPI
                     HLSVGIDSHEAMEKMIHFLVKKGYREIGLLSAKQDLSIFKQYLESWHTTLFAHHISPL
                     KIHHVVEDISFSAGATLLTEALLNWEAIDALVFLSDELACGALYEALRRHMSIPKDLA
                     IAGLGGLEVGSISYPKLTTIAIPYEQMGKIAGKQLLTLLSGKTLSAEEMIVKLPTKLV
                     IRDSAA"
     gene            1171..1584
                     /gene="secE"
                     /locus_tag="PXD81_000417"
     CDS             1171..1584
                     /gene="secE"
                     /locus_tag="PXD81_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HDL5536485.1"
                     /translation="MATEIKTKTPEQVEQKGGKSKGANTALWLVAIVFLAVAAVGNAY
                     FSSHFSLIVRVLLLVVLLVGAVALAAITNQGQKAIHFIKESRTELRKIIWPTRPEATQ
                     TTLIVLAMCVVVSLVLWGIDSIIVALITFVTSLRF"
     gene            1586..2149
                     /gene="nusG"
                     /locus_tag="PXD81_000418"
     CDS             1586..2149
                     /gene="nusG"
                     /locus_tag="PXD81_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HDL5536486.1"
                     /translation="MSEVEVKENGSKRWYVLQAFSGFENRVAVTLREYIKLHKMEEQF
                     GEVLVPTEEVVENVGGKRRKTERKFFPGYVLVEMEMNDDTWHLVKSVPRVMGFIGGTA
                     DRPAPITKREADRILNRVQETAEKPRHRKEFQPGESILVTEGPFADFSGTVEEVDYEK
                     GRLKVSVSIFGRATPVELEFSQVEKQS"
     gene            2382..2810
                     /gene="rplK"
                     /locus_tag="PXD81_000419"
     CDS             2382..2810
                     /gene="rplK"
                     /locus_tag="PXD81_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HDL5536487.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     RTESLEKGLPIPVVITVYADKSFTFVTKTPPAAVLLKKAVGIKSGSGKPNKDKVGTVT
                     QEQIRQIAETKAADMTGATIETKMKSIAGTARSMGLIVEE"
     gene            2815..3504
                     /gene="rplA"
                     /locus_tag="PXD81_000420"
     CDS             2815..3504
                     /gene="rplA"
                     /locus_tag="PXD81_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HDL5536488.1"
                     /translation="MAKLTKKMKAIKAGVDSTKAYDINEAVEVLKKFATAKFVESVDV
                     AVNLGIDSRKSDQNVRGATVLPHGTGRTARVAVFTQGANAEAAKAAGADLVGMEDLAE
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKSGQ
                     IRYRNDKNGIIHTTIGKADFSAEQLKDNLNALLAALTKAKPTTAKGIFIKKVSISTTM
                     GAGVAVDQASL"
     gene            3566..3985
                     /locus_tag="PXD81_000421"
     CDS             3566..3985
                     /locus_tag="PXD81_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5536489.1"
                     /translation="MLRLLSFCFIFLLSACTVLPQIKQLPPQVESVKSFKVENNGQQS
                     LLLIQFYENKWRWIQTDPLGAPIARVWLTKKGWEPDGFVMPNKQAQLLFTALVNALNP
                     HSPPFYLDKGWKIEREKSQFTIWLPDSSKWKVEILGE"
     gene            4072..5294
                     /locus_tag="PXD81_000422"
                     /pseudo
     CDS             4072..5294
                     /locus_tag="PXD81_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005544283.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase"
     gene            5287..5691
                     /locus_tag="PXD81_000423"
     CDS             5287..5691
                     /locus_tag="PXD81_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005557532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydratase"
                     /protein_id="HDL5536490.1"
                     /translation="MHKFTFPIEQIAPLVPHSGNMILLDRITAFGEDFLTAETNIRPD
                     NPLIKHGKLATYASIEIMAQAGEPVRLGYLLGTRKLHLHRQEIPIGSQLQIQIKMSIQ
                     DATGFGVFDCQLIDLADNQVLLEGALNVFSPK"
     gene            5791..7224
                     /locus_tag="PXD81_000424"
     CDS             5791..7224
                     /locus_tag="PXD81_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA binding domain-containing protein"
                     /protein_id="HDL5536491.1"
                     /translation="MQEAYDIQHLCTRKEDKSFDRKSARKEPKEILRHLIGFANADGG
                     LLVIGIEDNGEITGFNYQKAHQPEEFIHALHNLQKMPIPVTYQQIEVVNSRGLADFVL
                     LFEVTASSGRVITSHDDRVFLRSKDQTLELNFEQRLQLEYEKGQRAFEEQPIARADFT
                     DLDMDLLEQYRQKLHTDRSIEEILKARDLINKEGQITNACILLFGNNPTRYFPNARLR
                     FLRYEGKAPLTGRTFNVVKEFMLEEPIPKLLESAKAVLKSQLREFQRLDENGQFTKID
                     EYPEFAWLEGIVNAIAHRSYSNQGDCIRISMFDDRLEIFSPGGLPRPVTLENMYYTRF
                     SRNPKIARVLYEFGWVKELNEGVNRMAEEMNAYQLNTPSYFEPNENAVRLILENNIEH
                     RTLRALERIEEIFTQDIMATLNKNEVTILQYLYENEMITVKIASEILDRSKELSRRIL
                     KSLEQKNILTWHGLSPKDPTQYYTLKK"
     gene            7238..7966
                     /gene="fabG"
                     /locus_tag="PXD81_000425"
     CDS             7238..7966
                     /gene="fabG"
                     /locus_tag="PXD81_000425"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012771807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HDL5536492.1"
                     /translation="MNNTVLITGSSRGIGKAIALTLAQQGFDIVVHCRSRVEEAEAVA
                     QAVRNLGKNARVLQFDVADREKVRSILEADVEQHGAYYGVVLNAGLTRDNAFPALTDE
                     DWDSVLRTNLDGFYNVLHPIMMPMIRRRKAGRIVCITSVSGLIGNRGQVNYSASKAGI
                     IGAAKALAIELAKRKITVNCVAPGLIDTDILDENVPIEEILKMIPAGRMGDPDEVAHA
                     VAFLMDEKATYITRQVIAVNGGLC"
     gene            8029..9174
                     /locus_tag="PXD81_000426"
     CDS             8029..9174
                     /locus_tag="PXD81_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014167571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase"
                     /protein_id="HDL5536493.1"
                     /translation="MKRVVITGIGVVTAFGKEWAEIHGYQPPKHWNRKQLRSLGRVSQ
                     FVVEASERALAQAGLLDENGVIFDWLKDRRMGVACGSSTGSTNDIKDAAELLMTGKSD
                     GFNANTYVRMMPHTTAANIGIFFGLTGRIIPTSSACSSGSQGIGYAYEAIKYGMIPMM
                     LAGGGEEFCASKVYVFDSLYAASRRNDNPKGTPRPYDKDRDGLVIGEGAGMFVLEELE
                     HALARGANIIAEVVGYGANSDGSHVTRPQATTMQRCMELALKDAGIQPNQVGYVNGHG
                     TATEQGDIAETQATTAVFGKIPLSSQKSYLGHTLGACGALESWFSIEMMRDGWFAPTL
                     NLENVDERCGDLDYLQGDGREIHTDYVMNNNFAFGGVNTSLIFKRWK"
     gene            9188..10093
                     /locus_tag="PXD81_000427"
                     /pseudo
     CDS             9188..10093
                     /locus_tag="PXD81_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251927.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dialkylresorcinol condensing enzyme"
     gene            10116..11258
                     /locus_tag="PXD81_000428"
     CDS             10116..11258
                     /locus_tag="PXD81_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HDL5536494.1"
                     /translation="MLSNVYITNTSAFLPNAPVSNDAMEAVLGMVGETPSRVRKMILR
                     SNGIQHRYYAIDPITRQATHSSTELAVQAVKKLGNSATSATCLAVGTSYPDQIMPGQG
                     VMVHGLLDAEPMEVLTTAGVCVAGMAAMKHAYNAVRIGEHRAAIATASETASAIMRSE
                     HFRAEIDHKKLDDARPEIGFEKDFLRWMLSDGAGAVLLANQPNEKGLSLKIHWIDLVS
                     YANEMPVCMYAGAEFIDGNYTGWKAMSEAQRAECSAMAVKQDVKLLNENIVKYTVEKA
                     LERIIPKYKLNAEEIDYFLPHYSSGSFRDKLLEGLQRINFLIPQEKWFTNLTTKGNTG
                     SASIYIMLDEFIRTHDLKQGQKILCYVPESGRFSSCFMLLEAVVNS"
     gene            11307..11522
                     /locus_tag="PXD81_000429"
     CDS             11307..11522
                     /locus_tag="PXD81_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HDL5536495.1"
                     /translation="MDINDIPQDNSKIFNGERKVIYARNPQPEIRQMISSILACYAWD
                     ENNPFVAKSDARLKALAEVVGKAEIGD"
     gene            11803..12294
                     /gene="rplJ"
                     /locus_tag="PXD81_000430"
     CDS             11803..12294
                     /gene="rplJ"
                     /locus_tag="PXD81_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005694693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HDL5536496.1"
                     /translation="MALNLQDKQAIVAEVNEAAKGALSAVVADSRGVTVEKMTELRKA
                     AREAGVTMQVVRNTLLRRAVEGTEFECLTDTFTGPTLIAFSNEHPGAAARLFTEFAKT
                     NKEFELKGAAFEGKKQDVAFLATLPTYEEAIARLMGTMKEAAAGKLVRTLAALRDQME
                     AAA"
     gene            12353..12721
                     /gene="rplL"
                     /locus_tag="PXD81_000431"
     CDS             12353..12721
                     /gene="rplL"
                     /locus_tag="PXD81_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HDL5536497.1"
                     /translation="MSLTNEQIIEAIASKSVSEIVELITAMEEKFGVSAAAAVAAAPA
                     AGAAAAEEKTEFDVVLAEAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGISK
                     AEAEALKKELEEAGAKVEIK"
     gene            complement(12828..13658)
                     /locus_tag="PXD81_000432"
     CDS             complement(12828..13658)
                     /locus_tag="PXD81_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDL5536498.1"
                     /translation="MVQKPLNHYLEMQEHFLYVPYLHHHRRIRVLLPKDYHNEGWERY
                     PVLYMHDGQNVFYSKESYSGHSWKIIPTIKSHQELPKMIIVGIDNAGANRLNEYGPWK
                     TTTGSTPETRNAGGLGEEYAKWVVETVKPFIDIHYRTKPQQEHTLLAGSSMGGIITAY
                     MGSRYPHIFGHLGVFSLASWFSEPDFLRFTHQYPLQPNTKVFIQVGTNEGDEIDSHFI
                     SNTNQTYIDCSLNYYQALIRIGVPLDNIRLRIMANEIHHEMHWADHFVEFLHFSLLRK
                     "
     gene            13884..15425
                     /locus_tag="PXD81_000433"
     CDS             13884..15425
                     /locus_tag="PXD81_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HDL5536499.1"
                     /translation="MQASLQDILEVVKAKNLTYQQKLMSLGNIAERLFNPIELLGYTE
                     EEWEHIENQMICDLNEGYAIYRPRYILPDYNVYMQKGCQFLDLPPPTNLDEALDGLLI
                     LYSHVPSITTFPVYIGRLDQLLEPFITDEEQDYIKIKRFLNHIDKTISDSFCHANIGP
                     NDTKAGRLILKAVIELENITPNMTIRYDKTKTSREFAELAAKACLLVSKPSFANDAYY
                     IQDLGEEYGIASCYNALPECGGAYTLTRLRLGTIGRACASVEQMVNEMLPKVAKLALS
                     TMDKRHKFLVEESNFFESSFLVQEGFIKRTNFTSMIAIVGLADAVNHLLKQEGLNETF
                     GQSKRGDEIATLIMDKLQAVNDAHKGLYVERTNNQYLLHAQVGANNHAEDKMNTPAHR
                     IRVGEEPTLLAHLKQSAPFHKYFPSGTGDLFAFDQTYTDHLDAVVDIIDGSFAQGYRY
                     ITTYLKNTDLIRVTGYLVKKSEVERYRKGEAVLRDTTWYGSGTDDCAKVFDRQLRDEE
                     NVNAG"
     gene            15428..16216
                     /locus_tag="PXD81_000434"
     CDS             15428..16216
                     /locus_tag="PXD81_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S cluster-binding domain-containing
                     protein"
                     /protein_id="HDL5536500.1"
                     /translation="MAALSEIFVPLHRIIPFSNVEGQGNRTSIFLQGCKLNCLYCHNP
                     ETIPRYSKEAKQVSLQYLYDQVMEAVPFIRGVTISGGEPTIHHKKLVPLFEALREQGL
                     TCYLDSSGFFDFEATEPLINVTDKFLFDLKGDGAGLQTLCFDRKNQAGKVPEQALPNV
                     NHIKPENLARNLQNLAKLLPLDKVEEVRLVYLNEFYNAHHLLEKVADLLKPYPDVLLK
                     IIRVHTKGARDETGLARFVPTIEQTDELAAFAKRHGIQKVITIY"
     gene            16229..17486
                     /locus_tag="PXD81_000435"
                     /pseudo
     CDS             16229..17486
                     /locus_tag="PXD81_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005760733.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
     gene            complement(17565..19574)
                     /locus_tag="PXD81_000436"
     CDS             complement(17565..19574)
                     /locus_tag="PXD81_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HDL5536501.1"
                     /translation="MSAENMPSVIRFEQAVAKKDYESACTELLSILSKLDSNFGGISN
                     IELNMPEQIENLENDKAIYFCTRMAVAITRLFEDPALEISEHGAMRFLTLQRWIALIF
                     ASSPYVNADHILRTYNRNKESANPNTVDLDATLQALIKFCILYLPESNILLNLDAAWN
                     ASSDLTASLCFALQSPRFIGTSSAFAKRAAILQWFPEKLAQIENLNKLPSAISHDVYM
                     HCSYDIEANKHNVKRSLNAVIRRHLLSVGWEDRKIEQLGTRNNKPVMVVLLEHFHSSH
                     SIYRTHSTSMVAAREHFHLIGLGSDAVDEMGQQVFDEFHLLPQDGSLFDRLSFLKDIC
                     DKNNPAVFYMPSIGMDLTTIFASNTRLAPIQAVALGHPATTHSDFIEYVIVEDDYVGS
                     ESCFSEQLLRLPKDALPYVPSALAPQNVVYNLRENPEVIHIGIASTTMKLNPYFLEAL
                     KAIRDRAKVKTHFHFALGQSSGITHPYVERFIKSYLGNDATAHPHSPYDEYLNILHNC
                     DMMLNPFPFGNTNGIIDMVTLGLVGVCKTGPEVHEHIDEGLFKRLGLSNWLITQTAEE
                     YVTQAIRLAENHEERLAIRRDIIENNKLQTLFSGDPRPMGQIFLAKVQAWLADKNSKN
                     AEVEVKTKKVRKAATASQSAKKQTTSKTQTAKAEKDNTAKTETKS"
     gene            complement(19584..20921)
                     /gene="rimO"
                     /locus_tag="PXD81_000437"
     CDS             complement(19584..20921)
                     /gene="rimO"
                     /locus_tag="PXD81_000437"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HDL5536502.1"
                     /translation="MSASPNIGFISLGCPKNLVDSERILTELRADGYNIIPSYENADL
                     VIVNTCGFIDSAVQESLESIGEALEANGKVIVTGCLGAKENQIREVHPKVLEITGPHS
                     YEAVMNHVHKYVPKPEFNPYTSLVPKQGIKLTPKHYAYLKISEGCDHRCTFCIIPSMR
                     GDLASRSIVQVLDEAKRLVDAGVKELLIVSQDTSAYSLDRKKEEATKTVFWNGMPIKS
                     NLITLCEQLGSMGVWVRLHYVYPYPHVDDLIPLMAAGKILPYLDIPLQHASPKILKAM
                     KRPGSIERTLERIKKWREICPELTLRSTFIVGFPGETEEDFQLLLDFLKEAQLDRVGC
                     FKFSPVEGAVATDMPDQVPEEVKEERFHRFMQLQQEISAARLQAKIGKTVQVIIDDID
                     EEGIIGRSMAYAPEIDGVVYVDNPSNQLVTVGQIISVTITHADEYDLWGILNN"
     gene            complement(21076..21768)
                     /gene="mtnN"
                     /locus_tag="PXD81_000438"
     CDS             complement(21076..21768)
                     /gene="mtnN"
                     /locus_tag="PXD81_000438"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HDL5536503.1"
                     /translation="MKIGIIGAMAQEIEILRQVMVEPKVTELAGCKIFEGKINNTRVA
                     LLQSGIGKVAAAIGTTLLIQLTQPDMIINTGSAGGLDPDLNVGDILISTEVRYHDVDV
                     TAFGYEIGQLPANPPTFSPNEQLVELAQKEAKKAGYNVVSGLICSGDAFINGNDKINQ
                     IRQNFPNVVAVEMEAAAIAQVCHGFNLPFVIVRAVSDVADKESHLSFEEFLPLAAEKS
                     STIVLAMLNNID"
     gene            complement(21826..>22008)
                     /locus_tag="PXD81_000439"
     CDS             complement(21826..>22008)
                     /locus_tag="PXD81_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005618109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SlyX family protein"
                     /protein_id="HDL5536504.1"
                     /translation="LETKVAFQELIIEELNQALIEQQFAIDKLQTQIRHIAEKLKGAQ
                     LSQVASQAEETPPPHY"
BASE COUNT         6449 a         4466 c         4916 g         6177 t
ORIGIN      
        1 ttagccctat aatatggaac aattaggaaa atagtatggc aaatggaaca agtagaaatc
       61 gaagatcttc gggcagagtc actttagctg atgtagccaa agttgttggt gttgggcaga
      121 tgacggtatc tcgtgctttg cgtacaccag aaatggtgtc ggagccacta cgtaaacgga
      181 ttgaagaggc aatcattgaa ctaggatata ttccgcattt ttctgctcgg caattagctt
      241 ctgcagaatc acataatttg gtcattgtga cgacgtcgat tagctcaacg gaaaatacct
      301 taattttatc ggcattacgc aaaagcgtgg cggattccgc attgcaaatg gtgattttga
      361 tcgctgatca agaatcttgg tttcaagaac tgattaatca tgctcctcaa gcgatagtgt
      421 ttttgaattt agattgccct cctaatgtcc gtgagtggat taaaaacagc aatatcccta
      481 ccgtagaaat aggtgcgata aaaaaagaac cgattcatct cagtgttgga attgatagcc
      541 acgaagcaat ggagaaaatg atccattttt tggtgaaaaa aggctatcga gagattggat
      601 tgttatcggc gaagcaagat ttatccatct tcaaacaata tttagaaagt tggcatacga
      661 ctttatttgc acatcatatt tctcctttaa aaatccacca cgtagtagag gatatttcgt
      721 tttcggcagg agcaactttg ttgactgaag cccttctaaa ttgggaagca atagatgcat
      781 tggttttttt atccgatgaa ttggcttgcg gagcattata tgaagcatta cgccgacata
      841 tgagcattcc aaaagatctt gcgattgccg gtttaggtgg tttagaagtg gggagtattt
      901 cctaccctaa attgacaacc attgcgatcc cttacgagca aatgggaaaa atagcaggta
      961 aacaactact tactcttttg agtggtaaaa ccttatcagc agaagagatg attgttaaat
     1021 taccgactaa gctagtgatt cgtgatagcg ccgcataata ttgattttgc ataaaaatct
     1081 ataaaagatc gtggcaagtc ttgtagaaaa tgatagaatt taacgcttta aaataatttc
     1141 tgattttttt attcattttt taggttatat atggctactg agattaaaac aaaaaccccc
     1201 gagcaagtcg aacaaaaagg ggggaaaagc aaaggtgcaa atacagcatt atggctggtt
     1261 gcgattgtat ttttagcagt tgctgcggta ggtaatgcct atttttcatc tcatttttca
     1321 ttaattgttc gagtgctttt attggtggtg ttgctagttg gtgctgttgc actagcggca
     1381 attacaaatc aggggcaaaa ggcaattcat tttattaaag agtctcgaac cgaactacgc
     1441 aaaattatct ggccgacacg ccctgaagca acacaaacca ccttgattgt attggcaatg
     1501 tgcgtagtag tttccttggt tttatggggt attgactcaa tcatcgtcgc attaattaca
     1561 tttgtaacaa gtttaaggtt ctaaaatgag tgaagtagaa gtgaaagaaa atggctcaaa
     1621 acgctggtat gtattacaag cgttttcagg ctttgaaaac cgtgtggcag taacattgcg
     1681 tgaatacatt aagcttcaca aaatggaaga gcagtttggt gaagtattag tgccaaccga
     1741 agaagtcgtt gaaaatgtgg gcggtaaacg ccgtaaaacc gagcgtaaat tcttcccagg
     1801 ttatgtattg gttgaaatgg aaatgaatga cgatacttgg catttagtga aaagtgtgcc
     1861 tcgtgtaatg ggctttattg gtggtacggc agatcgcccc gcaccaatta ccaaacgtga
     1921 agcagaccgc attctaaacc gtgtacaaga aacagcggaa aaaccacgcc acagaaaaga
     1981 attccaaccg ggcgaaagca ttttggttac agaaggtccg tttgcagatt tctccggtac
     2041 ggtcgaagaa gtggattacg aaaaaggtcg cttaaaagta tcggtgtcga tttttggtcg
     2101 tgcgacaccg gtagaattag aattcagtca agtggaaaag cagagctaat ttcaattaat
     2161 ttcacacaca agcggttatt tttctcaatt ttttgcaaaa ttcagcggaa aaaagaccgc
     2221 ttgtaacaag atttattcaa caaagcgaaa gttttgcttg aaaaatagtc gcaaattctt
     2281 taaaatttgc gacctattac gagccagcct gctgactcgt attttttatg taacagggga
     2341 gctgattttc agcgttatca cccatttaga ggaaaattaa aatggcaaaa aaagtccaag
     2401 cctacgttaa actgcaagtt gcagcgggta tggctaaccc atcaccaccg gttggtcctg
     2461 cattaggtca acaaggtgtt aacattatgg aattctgtaa agcattcaac gctcgtactg
     2521 agagcttaga gaaaggttta ccgattccgg ttgttatcac agtttacgct gataaatctt
     2581 tcacattcgt taccaaaact ccaccggcgg cagtgctact gaaaaaagcg gtaggtatca
     2641 aatctggttc tggtaaaccg aacaaagata aagtgggtac agtaactcaa gagcaaatcc
     2701 gtcaaatcgc tgaaactaaa gcagcagata tgactggtgc gactatcgaa accaaaatga
     2761 aatcaatcgc tggtacagct cgctcaatgg gcttaatcgt agaggaataa tacaatggct
     2821 aaattgacta aaaaaatgaa agcaattaaa gctggcgtag attctactaa agcttatgac
     2881 atcaacgaag cagttgaagt gttaaaaaaa ttcgcaaccg ctaaattcgt tgagagcgtg
     2941 gacgttgctg taaacttagg tatcgattct cgtaaatcag accaaaacgt gcgtggtgca
     3001 accgtattac cacacggtac aggccgtacc gctcgtgttg ctgtgtttac acaaggtgca
     3061 aacgcagaag cagcaaaagc agcgggtgct gacttagtgg gtatggaaga tcttgcagag
     3121 caaatcaaga aaggcgaaat gaactttgat gttgttatcg cttctccaga tgcaatgcgt
     3181 gttgtaggtc aattaggtca agtattaggt ccacgcggct taatgccaaa cccgaaagta
     3241 ggtacagtaa caccaaacgt tgcagaagcg gttaaaaatg cgaaatctgg tcagatccgc
     3301 taccgtaacg acaaaaacgg tattatccac actactatcg gtaaagcaga cttctcagct
     3361 gaacaattaa aagataactt aaacgcattg ttagctgctt taacaaaagc aaaaccaaca
     3421 acagcaaaag gtatcttcat caagaaagta agcatctcta ctacaatggg tgctggtgtt
     3481 gcggttgatc aagcatcact ttaattttta atagaatcaa aagaccgtag tgttaatact
     3541 ctacggtctt tttctttagg tacgaatgtt aagattatta tctttttgct ttattttttt
     3601 gcttagtgct tgtactgtat tacctcaaat taagcaatta ccgccacagg ttgaatctgt
     3661 aaaatccttt aaagttgaaa ataatggcca gcaaagtttg cttctaattc aattttatga
     3721 aaataaatgg cgttggattc aaactgatcc tttaggtgct cctattgcca gagtatggct
     3781 tactaaaaaa ggttgggagc cagatggctt tgtaatgcca aataagcagg cacagctatt
     3841 atttacagct ttagtcaatg cattaaaccc tcattctcca cctttttatt tagataaagg
     3901 ttggaaaatt gagagggaaa aatcccaatt tacgatttgg ctaccagata gcagtaaatg
     3961 gaaagtggaa atattggggg aataagcgaa aacttgaacc tctatgcttg aatcacttga
     4021 atatgaattt tataatttta gtgccattac acaataatac tggaataaat aataatgaca
     4081 ctatatttat cagagcccgc catcaccagt ccgattggat atggcatcgt ggagcatttg
     4141 aatgttttaa ttaatgcgaa agtatctcct ttagatacga cttcacagcc ttatttacga
     4201 cataatattt caaatagttc accgaaattt ctagggcaag tctctgctag attgcgtgat
     4261 tttcctgttg aactatctat tgaacaccgc agccgcaata accaactgct atggcacgcc
     4321 cttgcagaaa ttgagccgaa aattaccgct tgtatcgagc aattcgggcg tgagcggatt
     4381 gcggtgatca tcggcacgtc cactaccggc gccgatgaaa atattcccgt cttcaaagcc
     4441 ggtgtgcagg gcgattatgc caccgagcag tttaatcaac agcaaattct cttctctgcg
     4501 ccgtctgatt tcgttgccga agtgtatggc ttaacggggc tgagttacgg catttccacc
     4561 gcctgcacct cagaggcaag agcgttaatt tctgctgccc gtttactgaa agccggcttg
     4621 tgcgatgcgg tgatttgcgg tggtgtggat accctttcgc ctttaactat cagcggattt
     4681 agctcgctgg aagtgttatc aaaccaacaa accaacccat tttccgctaa ccgtaacggc
     4741 attaacatcg gcgaaggcgc agcggtgttt gtgatgagcc gtgaaccact ggacgaacat
     4801 cgcattcagt tactgggtta tggttccagc tcggacgctt accatatgtc ctcacctcac
     4861 cccgaagggg aaggggcaat ttccgctttc caaaatgccc tgcacaatgc tgatttagac
     4921 gcagccgaca tcggctggat caacctgcac ggcacaggga cgcttcataa cgatcaaatg
     4981 gaaagcctcg ctgtggcaaa aatcttcggt aatcagaccg cttgcaccac caccaaaccc
     5041 tacaccgggc atacccttgg gctgccggtg cggtggaagc ggcaattttg tggggaatgc
     5101 tctgtcgcca acacaacccg accggcaagc tccctgcgca attatgggat aaacaagcgg
     5161 acgaaaacct gccgaacatt gcgatcaccg atgaacagag ccgttgggca gcaggcagac
     5221 ggattggcgc aagcagctct tttgcctttg gcggcaataa cagcgtactg attttgggag
     5281 aaagcgatgc ataaattcac cttccctatc gaacaaattg caccattggt tcctcacagt
     5341 ggcaatatga tcctactcga ccgcattacc gcattcggcg aggatttttt gaccgctgaa
     5401 accaatattc gccctgacaa tccgctgatc aaacacggaa aacttgccac ctacgccagc
     5461 attgaaatta tggcacaagc cggcgaacca gttcgcttag gctacctgct cggcactcgc
     5521 aaactgcacc tccaccgcca agaaatcccg attggctcac agctacaaat tcagatcaaa
     5581 atgtctattc aagatgccac cggcttcggc gtattcgact gccaactgat cgatttagcc
     5641 gacaaccaag tgcttttaga aggggcgctg aatgtgttta gtccgaaata gagttggtag
     5701 tagtaagtag tagtaagtag tagtaagtag tagttggtag tagttggtag tagttggtag
     5761 tagttggtag tagttggtag gaaattcgtt atgcaagagg cctatgacat tcaacatctt
     5821 tgtactcgca aagaagataa atcctttgat cgcaaaagtg cgagaaaaga accgaaagag
     5881 attttgcggc atttgattgg ttttgcgaat gcagacggcg gtttattagt tattggtatt
     5941 gaagataatg gcgaaattac cggttttaac taccaaaaag cccatcagcc ggaagaattt
     6001 atccacgctc tgcacaattt acaaaaaatg ccgataccgg tgacctatca gcaaattgag
     6061 gtggtaaata gtcgaggttt agcggatttt gtcttattat ttgaagttac agcttcgagt
     6121 gggagggtga ttacctctca cgatgatcgg gtttttctac gctcaaaaga tcaaactttg
     6181 gaactcaatt ttgaacaacg tttgcaatta gagtacgaaa aagggcagcg tgcttttgaa
     6241 gagcaaccga tagcacgcgc tgattttact gatctggata tggatttatt agaacagtat
     6301 cgacagaaat tacacacaga tcgctcaata gaagagattc tgaaagcccg agatttaatt
     6361 aacaaagaag ggcaaattac gaatgcctgt attttgctat ttgggaataa cccgacacgt
     6421 tattttccta atgcccgttt gcgttttttg cggtatgaag gtaaagcacc tctgaccggt
     6481 agaactttta atgtcgtcaa agaatttatg cttgaagagc ctattcctaa gttattggag
     6541 agtgctaagg cagtattaaa aagccaatta cgagagttcc aacgtttaga tgaaaacggg
     6601 caattcacaa aaattgatga gtatccagaa tttgcttggt tagaaggtat tgtcaatgcg
     6661 attgcacacc gtagttacag taatcaaggg gactgcattc gcatttcgat gtttgatgac
     6721 cgtttggaaa tttttagtcc gggtggatta cctcgccctg tgacgcttga aaatatgtac
     6781 tatacccgtt tttcacgcaa tccgaagatt gcacgagtgc tgtatgaatt tggttgggtc
     6841 aaagagctga atgaaggtgt taatcgtatg gcggaggaaa tgaacgctta ccaacttaat
     6901 acaccttctt actttgaacc caatgaaaat gcggtcagac tgattttaga aaataatatt
     6961 gaacatcgga cattaagggc attagaacga atagaagaaa tttttacgca ggatattatg
     7021 gcgacattaa ataaaaatga agttactatc ctgcaatatt tatatgaaaa tgaaatgatt
     7081 actgtaaaga ttgctagtga gatattagat cggagtaaag aattatctcg tagaatatta
     7141 aaaagtttag aacaaaaaaa tattctcact tggcacggct taagtccaaa agatccaacc
     7201 caatactaca cactcaagaa atagtaggaa atataagatg aataatacag ttctcatcac
     7261 cggctcaagc cgtggtatcg gtaaagcgat tgctttaacc cttgcacaac aagggtttga
     7321 tattgtggta cattgccgtt ctcgtgtgga agaagcggaa gcggtggcac aagcggtcag
     7381 aaatctcgga aaaaatgcca gagtgttaca gtttgatgtg gcggatcgtg aaaaagtgcg
     7441 gtcgatttta gaggcagatg tggaacaaca tggtgcctat tacggcgtgg tgctaaatgc
     7501 agggcttacc cgtgataacg cctttcctgc tttaaccgat gaggattggg atagcgtgtt
     7561 gcgtaccaat ttagacggtt tttataatgt cctgcacccg attatgatgc cgatgatccg
     7621 tcgccgtaaa gcaggcagga ttgtctgtat cacctcggtt tccggcttga tcggcaaccg
     7681 tgggcaggtc aattacagcg cttcaaaagc cggcattatt ggtgcggcaa aagcactcgc
     7741 catcgagctt gccaaacgta aaatcacggt aaactgcgtg gcgcccggct tgattgatac
     7801 cgatattctg gacgaaaacg tcccaatcga agaaattctc aaaatgatcc ccgcaggacg
     7861 aatgggcgat cctgatgaag tggcacacgc cgttgcgttt ttaatggatg aaaaagccac
     7921 ttacatcaca cgccaagtca tcgctgtgaa tggtgggttg tgttaacttt aggataataa
     7981 ttcgtagggg cggatccagt acccgcccga agccaaaaag agataaatat gaaacgagtc
     8041 gtcatcaccg gaattggtgt ggttaccgct tttggtaaag agtgggcgga aattcacggc
     8101 tatcaaccgc cgaaacattg gaaccgcaag cagttgcgca gccttgggcg agtgtcgcaa
     8161 tttgtggtgg aggccagcga gcgggcttta gcacaggccg gtttgttgga cgaaaacggc
     8221 gtgattttcg actggctaaa agacaggcga atgggcgtgg cgtgtggctc gtccacaggc
     8281 tctaccaatg acatcaaaga tgccgccgaa ttgctgatga ccggcaaatc ggacggtttc
     8341 aatgccaaca cctatgtgcg aatgatgccc cacaccaccg ctgccaatat tggcattttc
     8401 ttcgggctga cagggcggat tatcccgact tccagcgctt gctcctcggg cagccaaggt
     8461 atcggttacg cttacgaagc cattaaatac ggtatgatcc cgatgatgct tgccggcggt
     8521 ggagaagaat tttgtgcctc caaagtctat gtcttcgaca gcctctatgc tgccagccgc
     8581 cgcaacgaca acccaaaagg cacgccacgc ccctatgaca aagaccgaga cggcttagtg
     8641 atcggcgagg gagcgggaat gttcgtattg gaagagctgg aacacgcgtt agcacgaggg
     8701 gcaaacatta tcgccgaagt ggtgggctac ggtgcaaaca gtgacggttc ccacgtcacc
     8761 cgcccacaag ccaccacaat gcaacgctgt atggaacttg cccttaaaga tgccggtatt
     8821 caaccgaacc aagtcggcta tgtaaacgga cacggcacgg ccaccgagca gggcgacatt
     8881 gccgaaactc aagccaccac agcggtgttc ggcaaaattc cgctcagctc gcaaaaaagc
     8941 tacttagggc atacgctcgg tgcgtgtggt gcattggaaa gctggttcag cattgaaatg
     9001 atgcgagacg gctggttcgc cccaacactg aatttagaaa atgtcgatga acgctgtggc
     9061 gacttggatt accttcaagg tgacggacgg gaaatccaca ccgattatgt gatgaacaac
     9121 aatttcgcct tcggcggtgt gaatacctca ttgattttta aacgttggaa ataaccgttt
     9181 tcaaataatg aaaaagatat tattagttta ttactcccaa accgggcaac tcacccattt
     9241 ggcagaaaat tttgtacaat cgcttgaaca agcgggcgtt tttgtggaaa aactcgcaat
     9301 caaaccgcaa caggaatacc cgtttccgtg gcggtttatg cgttttttta acaccttccc
     9361 cgaaaccgtc cacctcacac cgccgccgat tgagcctctg ccctttcagc acgaaatata
     9421 cgatttggtg atcatcgcct attccgtgtg gtttctctcc cccagccagc cgattaccgc
     9481 ttttttacag agtgaacagg caaaaaagtg ttgaaggata cgcccgtcat cacgctgatc
     9541 ggctgtcgca atatgtggct gatggcgcag gaaaaagtga aaaaactgct tgccgagagt
     9601 gaggcgaagc tcattggcaa cgtggtcaaa gtggatcaat ctaacgactg ggcgagtttt
     9661 atcaccacac cgctttggat gctgaccggc aagaaaaaaa atcggcaagc tgccgagcgc
     9721 tggcattgcc gaaagcgaaa tggaagatgt aaaacgcttc ggggcaaaac ttttgcacat
     9781 ttttagccaa aaccaaccgc ttgacagcac ccttttccgc caaatgcaag cggtcaaaat
     9841 tgacgaaaaa ttgatgatga gcgaaaaagt cggtgcgaga agtttccacc tttggggcaa
     9901 attactgatc aagtgcggtc aaatttcacc gcacttacgc caagcggtac tctattttta
     9961 catcctcttt ttagtgacgt taattttaac cgtcgtcccg atttcaacgg tgattaaacg
    10021 gctattacgc ccactgctga aagaaaaatt agaaaaacaa aaacggtatt ttgccgagcc
    10081 ttcgggcgag taaacgtacc ctacacggaa actttatgct atctaacgtc tatatcacca
    10141 acacctccgc ctttttaccc aacgcccctg tgtccaacga tgcaatggaa gcggtgttgg
    10201 gaatggtggg ggaaacgcct tcccgagtgc ggaagatgat tttgcgttct aacggtattc
    10261 aacatcgtta ttatgccatt gacccgataa cccgccaagc gactcacagc agtaccgagt
    10321 tggcggtgca agcggtcaaa aaattgggaa attctgcaac atcagcgact tgcttggcgg
    10381 tggggacatc ttaccccgac caaattatgc cggggcaggg ggtgatggta cacggcttgc
    10441 ttgatgccga accgatggaa gtgctgacca ctgccggtgt gtgcgtggcg ggaatggcag
    10501 cgatgaagca cgcctataat gccgtacgca tcggtgagca tcgtgctgcg attgcaacgg
    10561 cttccgaaac cgcctccgcc attatgcgca gtgaacattt ccgtgccgaa atcgatcata
    10621 aaaaactgga cgatgctcgc cctgagatcg gttttgagaa agatttcctg cgttggatgc
    10681 tctcagacgg tgcaggggcg gtattgcttg ccaatcagcc gaacgaaaaa gggttgagcc
    10741 tcaaaattca ctggatcgat ctggtctctt acgctaacga aatgccggtt tgtatgtacg
    10801 ccggtgcgga atttattgac ggcaactaca ccggctggaa agcaatgagc gaagcccaac
    10861 gtgccgaatg ctcggcaatg gcggtgaaac aagatgtcaa actgctcaac gaaaacatcg
    10921 tcaaatacac ggtggaaaaa gcattggagc ggattatccc gaaatacaag ctcaacgctg
    10981 aagagattga ctacttcctg ccgcactact cgtccggttc cttccgagat aaattattgg
    11041 aaggtttaca gcggatcaat ttcctgatcc cacaggaaaa atggttcacc aacctgacta
    11101 ccaaaggcaa cactgggtca gcctcgatct acattatgct cgatgaattt atccgcaccc
    11161 acgatctcaa acagggacaa aaaatcctct gctatgtgcc ggaaagcgga cgtttttcgt
    11221 cttgttttat gcttttggaa gcggtggtaa attcgtaaaa tcgtagggac gggggctatt
    11281 cccgtccaac aatcatcgga aaacgtatgg acatcaatga cattccccaa gacaacagca
    11341 aaatcttcaa cggcgaacgc aaagtgatct acgcccgcaa tccgcaacca gaaatccgcc
    11401 aaatgatctc ctctattctc gcttgctacg cttgggacga aaacaacccg tttgtagcga
    11461 agtctgatgc acggttgaag gcattggcgg aagttgttgg taaagcagaa attggcgatt
    11521 aagtttgatt ttgtgcttta cagggtcgca gattatctgt ataatttgcg accttaactt
    11581 gcgagagcaa gctgatggtg cttagcctat ctaagccccg tctaagactg taggggtgaa
    11641 aacttaataa tcctacatag atggtgaaca gacagaattc tctgcttctg gacaccttag
    11701 gctcaaagaa gattgcagtt tgtagcaatg caagcggtaa tatttttggg attttttgta
    11761 aattctgctt gaaaaggcag agtgtatcag gagctaaaac caatggcatt aaatcttcaa
    11821 gacaaacaag cgattgttgc tgaagtaaac gaagctgcca aaggtgcact ttcagctgtt
    11881 gttgcggatt ctcgcggtgt aacagttgaa aaaatgactg agttacgtaa agcagctcgt
    11941 gaagctggtg tgacaatgca agttgtacgt aatactttat tacgtcgtgc agtggaaggc
    12001 accgagttcg agtgcttaac agatacgttt accggtccaa ctcttatcgc attctcaaat
    12061 gaacacccag gtgcagcagc acgtttattc actgaatttg cgaaaacaaa caaagagttt
    12121 gaacttaaag gtgcagcctt tgaaggtaaa aaacaagatg ttgcattctt agcaacctta
    12181 ccaacttacg aagaagcaat tgcacgttta atgggcacaa tgaaagaagc tgcggcaggc
    12241 aaacttgttc gcactcttgc agcattacgc gatcaaatgg aagcagcagc ttaattttaa
    12301 gcactcttct ttattcgttt aacaacttat tttactttag gaatcgattg ttatgtcatt
    12361 aactaacgaa caaatcattg aagcgattgc ttctaaatca gtatcagaaa tcgttgaatt
    12421 aatcactgcg atggaagaaa aattcggtgt ttcagcagcg gcagcagtag cagcagctcc
    12481 agcagcaggc gcagcggcag cagaagagaa aactgagttt gatgtagtat tagcagaagc
    12541 tggtgcaaac aaagttgctg taatcaaagc agtacgtggt gcaacaggtt taggcttaaa
    12601 agaagctaaa gacttagtag aatcagctcc agctgcatta aaagaaggca tctctaaagc
    12661 agaagctgaa gcacttaaga aagaattaga agaagctggc gcgaaagtag aaatcaaata
    12721 atttttgatt tattttgtca gaacttcaag ttcaacaaac cccgatggaa acatcggggt
    12781 tttgtttttt cagaattgtg taatgtagat atgattacct ttatccatta cttgcgcagc
    12841 agtgaaaaat gtaaaaattc cacaaagtga tctgcccagt gcatttcgtg gtggatttca
    12901 tttgccataa tccgcaggcg gatattgtct aacggcacgc caatccgaat cagtgcttgg
    12961 tagtaattga gcgaacaatc aatataggtt tggttcgtat tagagataaa gtgcgaatcg
    13021 atttcatcgc cttcgttagt gccaacctga ataaacactt tggtattggg ttgtaacgga
    13081 tattggtgcg taaaacgcag aaaatccggc tcactaaacc aagaggcaag cgaaaacacc
    13141 cctaaatgcc cgaaaatatg tgggtagcgg ctgcccatat aagcggtgat aatgccaccc
    13201 attgaactgc ctgccaataa ggtatgttct tgctgaggct tggtgcgata gtgaatatca
    13261 ataaagggtt taaccgtttc caccacccat ttggcatatt cctcccctaa gcctccggca
    13321 tttcgggttt caggggttga gccggtggtt gttttccacg ggccgtattc atttaagcga
    13381 ttagctcctg cgttatcaat gccgacaata atcatcttcg gtaattcttg gtgtgattta
    13441 atggttggaa taattttcca agaatgcccc gaataagact ctttgctgta aaacacattt
    13501 tgcccgtcgt gcatatataa caccggataa cgttcccaac cttcgttgtg ataatccttc
    13561 ggcaatagta cccgaatgcg gcggtgatgg tgtaaatagg gaacatataa aaaatgctcc
    13621 tgcatttcta aatagtggtt aagtggtttt tgtaccatca gtctgtcctt ttgcttaaat
    13681 tgtggcgtat tttacacctt aatcagtctg agttaaaaca agcggtcaga aaaagtcaaa
    13741 aatttgcaaa ttgctgttac ctaacgcaat cggttgcctt atgcttttga ggaatataaa
    13801 agagaaactg tgatctaagt cacagcaatt tttattcggt ttgactaaaa tccgaactat
    13861 cacttaatag agaggaaaat gttatgcagg cttcattaca agatattctt gaggtagtaa
    13921 aagcaaaaaa tctcacttat cagcaaaaat tgatgagttt aggcaatatt gctgagcggt
    13981 tatttaaccc gattgaacta cttggctaca ctgaagaaga gtgggaacac attgaaaatc
    14041 agatgatttg cgatttaaat gaaggttatg cgatttatcg cccacgttat attttgcctg
    14101 attataatgt gtatatgcaa aaaggttgcc aattcttaga tttacctccg ccaaccaatt
    14161 tagatgaagc cttagacggc ttgttaattc tgtattcaca cgttccgtcc attactactt
    14221 ttccggtgta tatcgggcgt ttagaccaac tgttagaacc gtttataacc gatgaagagc
    14281 aagattacat taaaatcaag cgtttcttaa atcatatcga taaaaccatt tccgattctt
    14341 tctgccacgc taacatcggg ccgaatgaca ccaaagccgg tcgtttaatc ttaaaagcgg
    14401 tgattgagct tgaaaacatt acaccgaata tgacgattcg ttatgataaa accaaaacat
    14461 cgcgtgaatt tgccgaactg gcggcgaaag cgtgcttgtt ggtgtccaaa ccgtcttttg
    14521 ctaatgatgc ctactacatt caagatttag gcgaagaata cggaattgca agctgttata
    14581 acgccttacc ggaatgtggc ggtgcttaca ctctgacccg cctgcgttta ggtactatcg
    14641 ggcgtgcttg tgcgagtgtt gaacaaatgg tcaatgagat gctgccgaaa gtggcaaaac
    14701 tcgccctttc cacgatggat aaacggcata aattcttggt ggaagaaagc aatttctttg
    14761 aaagtagctt cttggttcag gagggcttta tcaaacgtac taacttcaca agtatgattg
    14821 cgattgtagg tcttgctgat gcggtgaatc atttactcaa gcaagaaggt ttaaatgaaa
    14881 cctttgggca aagcaaacgt ggcgatgaaa ttgcgacgct gattatggat aagctccaag
    14941 cagtaaatga cgctcataaa gggctgtatg ttgaacgaac caataaccaa tatctgttac
    15001 acgctcaagt gggggcaaat aaccacgctg aagacaaaat gaacacgccg gcacaccgca
    15061 ttcgtgtggg cgaagagcca accttattgg cacacttaaa acagtctgct cctttccata
    15121 aatatttccc atccggtacg ggggatttat tcgcctttga ccaaacctac actgatcatt
    15181 tagatgcggt ggtggatatt attgacggct cgttcgccca aggttatcgc tacattacca
    15241 cttacctgaa aaatactgat ttgattcgag tgacaggcta cttggtgaaa aaaagcgagg
    15301 ttgaacgtta tcgcaaaggc gaagcggtat tgcgtgatac cacttggtat ggttcaggca
    15361 ccgatgattg tgcgaaagtg ttcgaccgcc agctgcgtga tgaagaaaat gtgaatgcag
    15421 ggtaacaatg gctgcacttt ctgagatttt tgtgccgtta caccgcatta tccccttctc
    15481 gaatgtagaa gggcagggca atcgtaccag catttttcta caaggttgca agctgaattg
    15541 cctgtattgc cataacccgg aaaccattcc acgttatagc aaggaagcaa aacaggttag
    15601 cttgcaatac ttgtacgatc aagtgatgga ggcagttccc tttattcgag gagtcactat
    15661 ttcgggtgga gagccaacca ttcatcataa aaaattagtg ccactgttcg aggcgttgcg
    15721 tgagcaaggg ctaacctgtt atttagacag cagcggcttt tttgattttg aggcaacaga
    15781 gccgttaatt aatgttaccg ataaatttct gttcgatctc aaaggagatg gggcgggctt
    15841 acaaacgcta tgctttgatc ggaaaaacca agccggcaaa gtgcctgaac aggctcttcc
    15901 aaacgtgaat catattaaac ctgaaaactt ggctcgcaat ttgcaaaatt tagccaaatt
    15961 attaccgctt gataaggtgg aagaagtacg tttggtttac ctcaatgagt tttataacgc
    16021 ccaccatttg ctcgaaaaag tggctgacct acttaaacct tacccagatg tactacttaa
    16081 aattattcgg gtacacacca aaggagcgag agatgaaaca ggcttggctc gttttgtacc
    16141 cactatcgag caaaccgatg aactggcagc atttgcaaaa cggcacggta ttcaaaaagt
    16201 tattactatt tactaatgga gacttacgat ggatatacta attagcataa ttggtgtttt
    16261 tgtgttatta ggcttaggtg ttttattatc aaataatcgc aaagccatta aatttcgcac
    16321 aattttgggc gctttggcga ttcaaattgg ttttgccgct cttattcttt actttccagc
    16381 gggaagaaat gcattgctag ctactgcaaa ttgtgtgagc aatattatta attatggtaa
    16441 cgaaggtatt agctttgtgt ttggcaattt agcgaatccg agtaatagca gcattggttt
    16501 tgtgtttgcg gttaaagtat tgcctattat tatcttcttc tctgcgttaa tttcaatgct
    16561 ttactacctt ggtgtgatgc aatgggtgat taaaattatt ggtggagcat tacaaaaaac
    16621 actaggcact tccaaggcag aatcaatgtc agcggcagct aattattttt gttggtcaga
    16681 cagaagctcc tctagttgta aaaccataca ttagccgtat gacagagtca gaattatttg
    16741 ccattatggt tggtggaact gcttcaattg ctggttccgt tatggctggt tatgccggaa
    16801 tgggcgttcc tttaacttat cttattgcgg cctcctttat ggctgcacca gctgggttac
    16861 tatttgctaa attaatgtat cctcaaacgg aaaccttgga gagtaaagca gatgacaatg
    16921 ttgaagttga aaagccaaat aacgtggttg aggccctagc taatggtgct agtgcaggtg
    16981 ttactttggc tattaatgtc ggtgctatgt taatagcatt tattgcagtg atcgctttat
    17041 taaatggctt tattggtggc attggtaatt ggtttggtta tgaggggcta accttacaaa
    17101 ttttattagg ttatttattc caaccacttg ctttcttaat cggtgtgccg tggcaagatg
    17161 ccgatattgc aggtcaaatg ataggtatga aacttgctgt taatgaattt gtcggatatc
    17221 ttgagtttgc taaatatcta cagcctgatg ctgtggtgca attaagcgat aaaactaagg
    17281 caataattac ttttgcttta tgtggttttg caaactttag agcaattgcg gtattgatta
    17341 gtggtatcgg aagtatggca ccaagtcggc gttcagatat tgctcgttta ggtttaaaag
    17401 cagtgattgg cggcacattg gcgaacttaa tgagtgcaac tatagcgggt ttctttattg
    17461 gtttaagtgg tgccacatta ggttgataca agcggttaaa ttgactcact tttttgcaaa
    17521 aagcccaagt aagttaaatg actacttggg ctttgtttaa tgaactagct cttagtttcg
    17581 gtttttgctg tgttatcctt ttccgctttg gctgtctgtg ttttcgaagt tgtctgtttt
    17641 tttgcggatt ggctagcagt agctgctttt cttactttct ttgttttaac ttctacttcc
    17701 gcatttttag agtttttatc cgctaaccat gcctgcactt tagctaagaa tatttgtccc
    17761 atcgggcgag gatcgccaga aaataatgtt tgtaatttat tattttcaat aatgtcgcgt
    17821 ctaattgcta agcgttcttc gtggttttcc gctaaacgaa tagcttgagt aacgtattcc
    17881 tctgctgttt gggtaattaa ccaattagat agcccgaggc gcttaaataa accttcatca
    17941 atatgttcat gaacttcagg gcctgtttta cagacaccaa ctaaaccaag cgtaaccata
    18001 tcgataatgc cgtttgtatt tccaaatggg aatggattta gcatcatatc gcaattatgt
    18061 agaatattta gatattcatc atatggcgaa tgtgggtgtg cggttgcatc atttcctaaa
    18121 tagcttttga taaaacgttc cacataagga tgcgtaattc cactagattg ccctaatgca
    18181 aaatggaaat gagtttttac cttcgcccta tcacgaatgg cttttaatgc ctctaagaaa
    18241 tatgggttta atttcatagt ggttgatgca attccgatat ggataacctc tggattttca
    18301 cgtaggttat agaccacatt ttgaggcgct aatgcggaag gcacataggg tagcgcatct
    18361 tttggaagac gtaataattg ttcactaaag cagctttcgg aaccaacata gtcatcttct
    18421 acgatgacat attcaataaa atcagagtgt gtagttgccg gatgacctag cgcaacagct
    18481 tgaattggtg ctaggcgagt gttactagcg aaaatagtgg ttaaatccat tccaatgcta
    18541 ggcatataga atacggctgg attattttta tcacagatat cttttaaaaa gcttaagcgg
    18601 tcaaatagtg agccatcttg tggtaataaa tgaaattcgt cgaatacctg ttgccccatt
    18661 tcatctacag catcactgcc tagcccaata agatggaaat gttcgcgtgc agcaaccatt
    18721 gaggttgaat gagtacgata aatagaatgt gatgaatgga aatgctccaa taatactacc
    18781 atgacaggtt tattatttct ggtaccaagc tgttcaattt ttctatcttc ccaaccgaca
    18841 gaaagtaaat gacgacgaat tacagcattt aacgaccgtt ttacattatg tttatttgct
    18901 tcaatatcat aactgcaatg catataaaca tcgtgtgaaa tggcgctcgg tagcttgttc
    18961 aagttttcaa tttgcgctaa tttttctggg aaccattgca aaatagccgc gcgttttgca
    19021 aaagcagagg atgtaccaat aaatctaggt gattggagtg caaaacaaag tgatgcggtg
    19081 agatctgatg aagcattcca tgcagcatct agattcaata aaatattaga ttccggtaag
    19141 tataaaatac agaacttaat tagtgcttgt aatgtggcat ctaaatcaac cgtatttgga
    19201 ttagcagatt ctttatttct attgtaagta cgcaagatat ggtctgcgtt tacgtatgga
    19261 gaactggcaa aaattaatgc tatccagcgt tgtaatgtca gaaaacgcat agcaccgtgt
    19321 tcggagatct ctaatgcagg atcttcaaac aagcgagtaa tcgctaccgc cattcgagta
    19381 cagaaataga tagctttatc attttctaaa ttttcaatct gctctggcat attcaattca
    19441 atattggaaa tgccaccaaa attactatct aattttgaaa ggatagacag tagctcggta
    19501 caagccgatt cataatcttt tttcgcgacc gcctgctcaa aacggatgac gctaggcata
    19561 ttttctgctg acataatgta tccttagttg tttaaaattc cccacagatc atactcatct
    19621 gcatgtgtaa tggttactga aataatttgc cctactgtca ctaattggtt tgaaggatta
    19681 tctacataga ctacaccgtc aatttccggt gcatatgcca ttgagcgacc gataatgcct
    19741 tcttcgtcaa tgtcatcaat aattacttgt acggttttgc cgatttttgc ttgtaagcga
    19801 gcagcggaaa tttcttgttg tagttgcata aaacggtgga aacgttcctc ttttacttct
    19861 tccggaactt gatccggcat atcggtagca acagcacctt ccactgggct gaatttaaag
    19921 caaccaacac gatctaattg tgcttctttt aagaaatcga gtaatagttg gaaatcttct
    19981 tctgtttccc ccgggaagcc gacaataaag gtagaacgca gagttaattc agggcaaatt
    20041 tcacgccatt ttttaatgcg ttctaaggtt ctttcgattg aacccgggcg tttcatcgct
    20101 tttaagattt tcgggctggc gtgttggagt ggaatatcca aatacggcaa aattttgccg
    20161 gcagccataa gcggaattaa atcatctacg tgtgggtatg ggtaaacata gtgcaaacgc
    20221 acccacacgc ccattgagcc taattgctca catagcgtaa ttaaattact tttaatcggc
    20281 ataccgttcc agaaaacggt tttggttgct tcttcttttt tgcgatctaa cgaataggct
    20341 gaggtgtctt gtgagacgat taacagctct tttaccccgg catcaactag gcgttttgct
    20401 tcatctaata cttgcacgat agaacggctt gctaaatcgc cacgcattga aggaataatg
    20461 cagaaagtgc agcgatgatc gcagccttcg gaaattttca aataagcgta atgcttcggt
    20521 gttaatttaa tcccttgttt tggcactaag ctggtgtaag ggttaaattc cggttttggc
    20581 acatatttat ggacgtggtt cattaccgcc tcatagctat gtggccctgt aatttccaat
    20641 actttagggt gtacctcacg aatttggttt tcttttgccc ctaaacagcc ggtgacaatt
    20701 acttttccgt tagcttctaa cgcttcacca atgctttcaa gagattcttg tactgcggaa
    20761 tcaataaaac cgcaggtgtt tacaatcaca agatccgcat tttcataact agggatgatg
    20821 ttataaccat ccgcacgcag ttcggtcaaa atacgttcgg aatcaactaa atttttaggg
    20881 cagcctaagc tgataaaacc aatatttggt gaagcactca tataactctc aataactaaa
    20941 aagaaaaatg cctacaattt ttgtctgtag gcaaaaaact aacaagattc tacaaaatct
    21001 tgcccaaaat agcgataaca agcggtcaat ttttaataaa catttacaaa cttgaaattt
    21061 cgctaacaat taaaattaat cgatattatt tagcattgct aatacaatcg tggaagattt
    21121 ttctgcggct aatggcagaa attcttcaaa agaaaggtgg gattctttgt ccgctacatc
    21181 cgatactgct cttacgatta caaatggaag gttaaaaccg tgacaaacct gcgcaatggc
    21241 tgctgcttcc atttcgactg caacgacatt cgggaaattc tgacgaattt gattaatctt
    21301 atcattaccg ttaataaagg catcgccgct acagattaag cctgaaacca cattgtaacc
    21361 ggcttttttc gcttcctttt gagctaattc gactaattgc tcgtttggcg aaaatgttgg
    21421 cgggtttgcc ggtagttgcc cgatttcata accaaaggcg gtcacatcaa catcgtgata
    21481 acgaacttcg gttgaaatca aaatatcacc tacgtttaaa tctggatcta atccaccggc
    21541 agagcctgta ttaataatca tatccggctg ggtgagctgt atcagtaagg tagtgccgat
    21601 ggcggcagca actttgccga tacccgattg taataatgcc acacgggtat tgttaatttt
    21661 gccttcaaaa attttacaac ctgctaattc agtgacttta ggttcaacca ttacttggcg
    21721 taaaatctca atttcttgtg ccatcgcacc aataatacca attttcatta ctttccttct
    21781 ctaatgtatt gtttattttt tgaaaaaaac gctattttaa cagcattaat agtgaggagg
    21841 gggtgtttct tctgcttgtg aagcaacttg gcttagttga gcccctttca atttctcagc
    21901 aatatggcga atctgagttt gtaacttatc aattgcaaat tgttgctcaa taagtgcttg
    21961 atttaactct tcaataatca gctcttggaa agcaacttta gtctccaa
//
LOCUS       DANLYI010000014        21500 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.014, whole genome shotgun sequence.
ACCESSION   DANLYI010000014 DANLYI010000000
VERSION     DANLYI010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 21500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21500
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.014"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            complement(<1..391)
                     /locus_tag="PXD81_000440"
     CDS             complement(<1..391)
                     /locus_tag="PXD81_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HDL5536505.1"
                     /translation="MQLTFNDFQNYYNGKGDLTMFSFYGKKTWENGIYVGIDGSIGRA
                     NNKLSHQSQTIKLARNVYSLGLTVGKTLEVASFNLQPSFGMRYHHLTSANAQLENTKF
                     ETDRVDLLAIQAGLALNKTFIFNELKVK"
     gene            complement(440..1255)
                     /gene="cysE"
                     /locus_tag="PXD81_000441"
     CDS             complement(440..1255)
                     /gene="cysE"
                     /locus_tag="PXD81_000441"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015674390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HDL5536506.1"
                     /translation="MQQSEINQIWQAIRDEAKELANCEPMLASFFHATILKHHNLGDA
                     LSYILANKLENPIMPAIALKEIIEEAYRAEPQIIASAACDINAVRTRDPAVDKWSTPL
                     LYLKGFHALQSYRVTHYLWNQGRKALAVYLQNEISVAFDVDIHPAAKVGCGIMFDHAT
                     GIVVGETSVIENDVSILQGVTLGGTGKENGDRHPKIREGVMIGAGAKILGNIEIGRYS
                     KIGANSVVLQPVPDHATAAGVPARIIGKSSEQKPAFDMNQYFEDEQGLFGDGI"
     gene            complement(1271..2281)
                     /gene="gpsA"
                     /locus_tag="PXD81_000442"
     CDS             complement(1271..2281)
                     /gene="gpsA"
                     /locus_tag="PXD81_000442"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012263265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HDL5536507.1"
                     /translation="MSEIYSAPITVLGAGSYGTALAIALSRNGHKTYLWGHNPDKMAV
                     LASERMNQDFLPDIVFPEALEIVADLATAVQKSKDLLIVVPSHVFSEVLAQIKPFLSQ
                     DHRIMWATKGLERDTGRLLQTVAKEILGEIYPLAVLSGPTFAKELAAGLPTAISLAST
                     DPVFADQMQKRSHCSKAFRVYLNNDIIGVQLGGAIKNVIAIGAGISDGMGFGANARTA
                     LITRGLAEISRLGVSLGADPKTFTGMAGLGDLVLTCTDNQSRNRRFGLALGQGKSAEV
                     AIAEIGQVVEGFYNTKEAYLLAQTQGVEMPIVEQIYQMLFCGKQASDVVKTLLGRERK
                     GE"
     gene            complement(2388..2888)
                     /gene="secB"
                     /locus_tag="PXD81_000443"
     CDS             complement(2388..2888)
                     /gene="secB"
                     /locus_tag="PXD81_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HDL5536508.1"
                     /translation="MSEENQVASTEEATQTPFELQIQRIYVKDVSFEAPNLPTIFQQE
                     WKPQLGFELDTETKEIGEDLYEVTLHITVQTTLEDSNDVAFICEVKQAGVFTIKGLEG
                     IQLAHCLASQCPNVLYPYARELISSLVNRGTFPALNLSPVNFDALFMDYLQRQEESAE
                     EPALAN"
     gene            complement(2909..3361)
                     /locus_tag="PXD81_000444"
     CDS             complement(2909..3361)
                     /locus_tag="PXD81_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HDL5536509.1"
                     /translation="MEQLTFAQQLEQFFANHTLMVFAWVALLIAVIFSFYKSATQKYK
                     IVDNAQATQLINKEEAVVVDLRSDEEFRAGHIIDSIHVLPSDIKAAKINQIDKYKARP
                     VIVVDTNDFTSGGVAADLAKQGFSRVYVLKEGIGGWRAASLPTIKKHK"
     gene            complement(3477..4775)
                     /gene="tig"
                     /locus_tag="PXD81_000445"
     CDS             complement(3477..4775)
                     /gene="tig"
                     /locus_tag="PXD81_000445"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005602169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HDL5536510.1"
                     /translation="MSFSIETLEGLKRRVTINVPADKIEAAYKEQLKGYAKNARVDGF
                     RKGKVPHSIIEQRFGIAARQDVLSDEMQRAFFEAVIAEKINLAGRPTFTPNNYQPGQD
                     FSFTAEFEVFPEVELKGLENIEVEKPVVEIADADLDKMVDVLRKQQATWSESQEAAKA
                     ESRVTIDFVGSVDGEEFEGGKASDFVLFMGQGRMIPGFEDGIVGRKAGEQFDIDVTFP
                     EEYHAENLKGKAAKFAITLKKVEEMVLPELTEEFVKKFGNAKSVEELRAEIKKNMQRE
                     LKNAVTSRVKTQVINGLIAQNDVEVPAAAVEEEINVLRQQAVQRFGGKPEMAAQLPAE
                     LFEAEAKRRVQVGLLLSSIIGSNELKVDEKRVEEMIADIASAYEQPAEVVAHYAKNRQ
                     LTENIRNVVLEEQAVDAVLAKAKVTEKTMSFDEVMSQQAQ"
     gene            complement(5072..6256)
                     /gene="tuf"
                     /locus_tag="PXD81_000446"
     CDS             complement(5072..6256)
                     /gene="tuf"
                     /locus_tag="PXD81_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011199348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HDL5536511.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKHFGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDTATRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVAATDGPMPQTREHILLGRQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQ
                     YDFPGDDTPIVRGSALQALNGVAEWEEKILELANHLDTYIPEPERAIDKPFLLPIEDV
                     FSISGRGTVVTGRVERGIIRTGDEVEIVGIKDTAKTTVTGVEMFRKLLDEGRAGENVG
                     ALLRGTKREEIERGQVLAKPGSITPHTDFESEVYVLSKEEGGRHTPFFKGYRPQFYFR
                     TTDVTGTIELPEGVEMVMPGDNIKMTVSLIHPIAMDEGLRFAIREGGRTVGAGVVAKI
                     IK"
     gene            complement(6316..8418)
                     /gene="fusA"
                     /locus_tag="PXD81_000447"
     CDS             complement(6316..8418)
                     /gene="fusA"
                     /locus_tag="PXD81_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HDL5536512.1"
                     /translation="MARTTPISLYRNIGISAHIDAGKTTTTERILFYTGVSHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMSQQYPQHRINVIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRTGANFLRVVEQIKT
                     RLGGNSVALQLPIGAEDSFKGVVDLIKMKAINWNEADQGMTFTYEDIPADMLEACEEH
                     RAMLVEAAAEASEELMEKFFSGEELTEDEIKAALRQRVLAGEVIPVCCGSAFKNKGVQ
                     AMLDAVIDYLPAPTDIPAIKGINEDETEGERHASDDEPFASLAFKIATDPFVGNLTFF
                     RVYSGVINSGDTVYNSVKQKRERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVGTG
                     DTLCAIDAPIILERMEFPEPVISVAVEPKTKADQEKMGLALGRLAQEDPSFRVHTDEE
                     SGETIISGMGELHLDIIVDRMKREFKVEANIGKPQVSYRETIRTRVNDVEGKHAKQSG
                     GRGQYGHVVIDLYPLDAEGPGYEFVNEIKGGVIPGEYIPAVDKGIQEQLKSGPLAGYP
                     VVDLGVRLHFGSYHDVDSSELAFKLAASLAFKAAFAKASPVLLEPIMKVEVETPPDYV
                     GDVIGDLSRRRAMVNGQEANEFVVKINAEVPLSEMFGYATDLRSQTQGRASYSMEPLK
                     YAEAPKNVADAIIEARRK"
     gene            complement(8520..8990)
                     /gene="rpsG"
                     /locus_tag="PXD81_000448"
     CDS             complement(8520..8990)
                     /gene="rpsG"
                     /locus_tag="PXD81_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007526570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HDL5536513.1"
                     /translation="MPRRRSIEPRKILPDPKFGSELLAKFINVLMVDGKKSTAEKIVY
                     GALETLTQRSGKEALEAFEIALENVRPTVEVKSRRVGGSTYQVPVEVRPTRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAADNKGSAVKKREDVHRMAEANKAFAHFRW"
     gene            complement(9121..9495)
                     /gene="rpsL"
                     /locus_tag="PXD81_000449"
     CDS             complement(9121..9495)
                     /gene="rpsL"
                     /locus_tag="PXD81_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HDL5536514.1"
                     /translation="MATINQLVRKPRVKKVVKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRIRLTNGFEVTSYIGGEGHNLQEHSVVLIRGGRVKDLPGVRYHTVRGAL
                     DCAGVKDRKQGRSKYGVKRPKA"
     gene            complement(9616..10575)
                     /gene="rfaC"
                     /locus_tag="PXD81_000450"
     CDS             complement(9616..10575)
                     /gene="rfaC"
                     /locus_tag="PXD81_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HDL5536515.1"
                     /translation="MKILIVKTSSMGDVLHTLPAITDMQNAIPDLKVDWVVEENFTEI
                     PAWHSAVNKVIPVAIRRWRKNLCQRQTWVEWQQFLKQLKSEQYDAVIDAQGLIKSAVL
                     VTRQAKGKKFGYDKNSAREGLSSLFYDETFNIPYQQHAVERIRKLCAKSLGYALPTER
                     GDYCIAQHFAKNPQKTTAYIMLIHATTRADKHWQEAYWAELIQALDKHNLAVRLPWGN
                     QAEKERAERLASVANNAKVLPKMSLTDIATQLAGAAAVVSVDTGLSHLAAALDKPNVI
                     LYGATDPKLIGAYGQNQFYLKADKMENITSSQVLEKLLPLINK"
     gene            complement(10642..11679)
                     /gene="waaF"
                     /locus_tag="PXD81_000451"
     CDS             complement(10642..11679)
                     /gene="waaF"
                     /locus_tag="PXD81_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HDL5536516.1"
                     /translation="MNILVIGPSWVGDMMMSHALYQQLKIQYPNCQIDVMAPDWCCPL
                     LARMPEVRNAISMPIGHGSFRLCERYRLGKSLRNQYDMAIVLPNSLKSAFIPLFAKIA
                     RRRGWKGESRYFFLNDLRSNKNDYPMMVQRYVALAFEKATVPTAQQLPIAYPYLTTDS
                     EQIEQTKAKFEKQLAYAENRPAIGFCPGAEFGPAKRWPHYHYAILAKMLIEQGYSVRL
                     FGSKKDEAAGEQIRLALPENLQRYCLNLAGQTDLNQAVDLIADCNAVVSNDSGLMHIA
                     AALNKPLVALYGPTSPQYTPPLSQNAEIMRLIEGGLIKIRKGEGAEGYHQSLIDIQPE
                     MVMDKLAELLV"
     gene            complement(11790..12152)
                     /locus_tag="PXD81_000452"
     CDS             complement(11790..12152)
                     /locus_tag="PXD81_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MliC family protein"
                     /protein_id="HDL5536517.1"
                     /translation="MLVACSTVVTPVEQIHQVQKEKESVKKVTKLIAQAPKVSLYRCE
                     NDKKVEVERQGRTKKALKKEIVTVSYQGTTHQLSPSVTRDGKKYTNIRWTWHETRSGK
                     AFLYNNTKKTLAANCIKQ"
     gene            complement(12270..13589)
                     /locus_tag="PXD81_000453"
     CDS             complement(12270..13589)
                     /locus_tag="PXD81_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="opacity-associated protein A"
                     /protein_id="HDL5536518.1"
                     /translation="MTQNNFRKEPVFGESSVTSQDAEATKTDTAQAPYVSLRSTKAPG
                     HTFTPIMKRPDAKVEDHTADVFSAKQAKEFQFTPSSEATEAVEPDNTIHSVEESVSTA
                     SFNAVEAQENVEKEVVKPAPESFTTERIIPNAATAAAAATAAAAATKTKEIKAKMPPK
                     TRRLLLVALLALALLVLFFLLKPSTPETVEELQSQQGGSLPIEFRPVDEAEAKRAEEQ
                     ARAEQEAAEAAKAQEQAQAAQQESVTVAPAQTEQTMAKPSQDSQSQTQSQAVVEQPVA
                     VKPVENKPVVAQPAIKPKTENSVVFQPETTVKQQPKAPAKTEVKTERATLAAKETAKT
                     AAKPVEKAPTKTEAVKAAPAAESANAVATKTMTVPKGVSLMQVFRDNNLNISDVNAMS
                     KVNNVVSNLKVGERVTVRLDKNNRVVEMSIGSGGKFTRQANGSYTFK"
     gene            13882..14220
                     /locus_tag="PXD81_000454"
     CDS             13882..14220
                     /locus_tag="PXD81_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEA family DNA-binding protein"
                     /protein_id="HDL5536519.1"
                     /translation="MKKIKQLGLTFLFGLNAMAFAQTPPTTMPVEQNAVTQPVQQTQI
                     TNANAVNINTASAAELQDKLVGIGQKKAQAIVDYRTKNGNFKNIEQLAEVSGIGQATV
                     EKNRANIILE"
     gene            14385..15278
                     /gene="psd"
                     /locus_tag="PXD81_000455"
     CDS             14385..15278
                     /gene="psd"
                     /locus_tag="PXD81_000455"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251643.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HDL5536520.1"
                     /translation="MQLKKYPVPTYFQRVKIALHYLLPQLAITRAAGWLAEQKWGVVT
                     HLIIKLFAKQYKVNLSEAKKTEPSDYATFNEFFIRELKENARPINQDPNTLCLPAEGK
                     VSESGEISDNRLLQAKGHHFTLETLLANDEEMAAKFKDGTFITTYLSPTDYHRVHMPC
                     DGTLRKMIYVPGELFSVNPFLAEHVPNLFARNERVICEFDTAFGPMLQILVGATVTAS
                     MSTVWAGVINPPRADEVVVWNYETEGEKAIKLQKGEEMGAFRLGSTVINLFPKGKVHL
                     NPALIAGMQTRMGEELGRIIN"
     gene            15350..17701
                     /locus_tag="PXD81_000456"
     CDS             15350..17701
                     /locus_tag="PXD81_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005608625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HDL5536521.1"
                     /translation="MYTLQELKQMNEQEIRSRLISPAIRNAGWSDRQIGEEYTFKTNK
                     RFTDGQVVVDPKTTQTKRIEAKRVDYLLYTSANQKIAVVEAKDNHHSSRHGLQQAMTY
                     ARLLDVPFAYSSNGDEFVEHDFITGVQRTLPMSAFPTPDELHQRWAKQYTTEELKIIQ
                     QPYYTSSTDTFKEPRYYQEIAINRTVEAVAKGQQRILLVMATGTGKTYTAFQIIHRLK
                     TSGAKKKILFLADRNVLVDQPMMQDFKPFKDKMVKVSNKKLDSAYEVYLALYQQLDGE
                     TDNELFKQFQPSFFDLIVIDECHRGSAKEDSDWRAILKYFKDATHIGMTATPKETDDA
                     SNITYFGEPIYTYSLKQGIEDGFLAPYRVIRVDLDCDLKGWRPTKGLKDHQGNEIEDR
                     LYTVKDFDYNIVLNDRTRLVAERICEFLRKTDRLQKTIVFCANINHAERMVAELSRLN
                     ADMLKHNSKYVCQITGDQSGKGVDLEEFCDVANEGKDYPVIAVTSKLMTTGVDSKTCK
                     LVVLDANIQSMTEFKQIIGRGTRLRPDFGKEFFTILDFRGVTRLFADPAFDGEPVVIL
                     DVPENKPLVEQPLPKPSDKDIIKDPELKPYKIPVVVDFGTLKLRILDEKVQYLNEQGE
                     LVSEDIDLFSKREMQKEFGSYEQFQQQWTTSGEIFSKFYTDPKWLAALRQTRQFSHDV
                     EDFDVLSHISFGKKPLTKTERAEKVKQSGYVEQYSPENQQVLGLLLNEYVKSGNQDLT
                     KTEILKTPQFSRFGGLVSIIKNFGGKELYQKAVKGLEKMIYSE"
     gene            17749..19218
                     /locus_tag="PXD81_000457"
     CDS             17749..19218
                     /locus_tag="PXD81_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005605245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HDL5536522.1"
                     /translation="MSLNNIVKRLQDVMRNDAGINGDAQRIEQIVWILFLKIYDAKEQ
                     EWELENDEYHSILPNFLRWQNWAEDKKDGKAMTGDELLSFVNNELFPTLKNLPISADT
                     PMNQRIIRAAFEDNNNYMKNGVLLRQVINIIDEIDFAHYQERHAFGDIYENILKSLQS
                     AGNAGEFYTPRAVTDFMVQAIKPKLGERIADFACGTGGFLTSALKVLESQIQTLSDRT
                     LFNNSVYGIEKKALPHLLCITNLLLHDIDNPNVHHDNALEKSVKDYTENDKFDVILMN
                     PPYGGSEIEQIKKNFPTALQSSETADLFMSVIMYRLKQNGRAAVVLPDGFLFGTDNAK
                     VAIKKKLLEEFNLHTIIRLPHSVFAPYTSITTNILFFDKTEPTRETWVYRLDMPQGYK
                     NFSKTKPMKLEHFNEVVEWWGNRQVIEVEGFDKAKSFTLKEMSDRQYNLDLCGFPHEE
                     EEILPPDELIADYQRKRTAYNADIDKILAEITDILGIKL"
     gene            19231..20892
                     /locus_tag="PXD81_000458"
     CDS             19231..20892
                     /locus_tag="PXD81_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA binding domain-containing protein"
                     /protein_id="HDL5536523.1"
                     /translation="MLTWEHIEKMIAHQQFIREKAEIEYKEAKNSVPKDLWHTYSAFA
                     NSNGGFILLGIKENEQGHFPELLISGVNDPDKVIDELFSQSRSEKVSKSLLKDNDVKI
                     FQVENKSVIAIYVKAADILDRPIHLNGDYRLSYVRLNSGDHKLTSSELRSFISSYSQK
                     DADGRIVPYASIDDISLSTLDKYRNLLKAHNSTSKLLTLSDEKLLQEIGAYKKDLAQN
                     SEGITNAGLLMFGKHYAITSIFPHFFFEYYEEAGDVKRYTLRITDFDLEEGNLFEFYL
                     KIVPLIQELGKDKHFKLDNLTRTEENEITDGLREALINAITHADYFNNVRHLKVIKTK
                     DNKLIFENAGVMLVDIPTAIAGQRSECRNSIIHNLFHRLGLCERQGQGVRDIYSYWQS
                     RNFNSPLLESEIDYTKLTLTFQSGMIADFEEKFTACFGENFSELQPIQKDCLLFIAFN
                     QGKAMFAELAQKLPQYPRRNITLALARLKEKGLLTSSGSPRKQLYTLVNLEWIITEKS
                     QDDYFNPPFDQLDKASESKGAPPKKSKVKQVKQLPQNKANKLPPK"
     gene            21098..21340
                     /locus_tag="PXD81_000459"
                     /pseudo
     CDS             21098..21340
                     /locus_tag="PXD81_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012478544.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
     gene            21317..>21500
                     /locus_tag="PXD81_000460"
     CDS             21317..>21500
                     /locus_tag="PXD81_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HDL5536524.1"
                     /translation="MWVRLGDICLKITDGTHHSPPNIDKSDFLYITAKNIKKDGLDLS
                     KISYVTKEIHNEIFSRC"
BASE COUNT         6276 a         4608 c         4122 g         6494 t
ORIGIN      
        1 gttttacctt taattcatta aatataaaag ttttatttaa tgctaaacca gcttgaatgg
       61 ccaaaagatc taccctatca gtctcaaatt ttgtgttctc taattgggca tttgcagatg
      121 ttaaatgatg atagcgcata ccaaatgacg gttgtaggtt aaaacttgca acttccaacg
      181 ttttacctac cgttaatcct aaagaatata catttctggc tagttttatc gtctggcttt
      241 gatggcttaa tttgttattc gcacgcccaa tgctaccatc aatccctaca taaatgccat
      301 tttcccatgt tttcttacca taaaaagaaa acatagttaa atccccttta ccattatagt
      361 agttctgaaa atcattaaag gttaattgca acttgtactt agcaatgtct aaatcataaa
      421 ttaaaggaat aactcttacc taaatcccat caccaaacaa accttgctca tcttcaaagt
      481 attgattcat atcaaaagcc ggtttttgtt cagaggattt gccgataatg cgagccggta
      541 cacctgctgc tgtggcgtga tcaggcacag gttgtagcac cactgaattt gccccgattt
      601 tagaatagcg tccgatttca atattaccga gaatttttgc accggctcca atcatcacgc
      661 cttcacggat tttcgggtgg cggtcgccat tttctttccc tgtaccgcct agtgttacac
      721 cttgtaaaat cgagacatca ttttcgatca cagaggtttc acccaccaca ataccggtgg
      781 cgtggtcaaa cataattccg caaccgacct ttgccgcagg atgaatatcc acatcaaagg
      841 caacagaaat ttcattttgt aaataaaccg ctaatgcttt cctgccttga ttccaaagat
      901 aatgcgtcac ccgatagctt tgtaaagcgt ggaagccttt taagtacagc agaggtgtac
      961 tccatttgtc caccgcagga tctctagttc tgaccgcatt aatatcacaa gctgcactag
     1021 caataatttg tggctctgct cgataagctt cctcaataat ttcttttaag gcgattgccg
     1081 gcataatcgg gttttctaac ttattcgcca aaatataact taacgcatcg cctagattat
     1141 ggtgttttaa aatggtagcg tggaaaaaac ttgctaacat aggttcacaa ttcgccagct
     1201 ctttggcttc atctcgaatt gcttgccaaa tctgattaat ttcactctgc tgcattttat
     1261 tctcctttct ttattcgcct tttcgttcac gccccaacaa ggttttcacc acatccgaag
     1321 cctgtttgcc acaaaataac atttggtaaa tttgttctac aatcggcatt tcaacgccct
     1381 gtgtttgagc gagcaaatag gcttctttgg tattataaaa tccttctacc acttgaccga
     1441 tttccgctat cgcgacttct gccgattttc cttgccctaa cgccaaacca aatcggcggt
     1501 ttcgggattg gttatcggta cacgtcagca ctaaatctcc aagccccgcc attccggtaa
     1561 aggttttcgg atcagccccg agcgaaacac ccaaacggct aatctccgcc agccctcgag
     1621 taatcaaggc ggtacgagca ttcgccccga atcccattcc gtctgaaata ccggcaccaa
     1681 ttgcaatcac atttttaata gccccaccga gttgcacacc gataatatca ttgttgaggt
     1741 aaactcggaa cgctttggaa cagtgactcc gtttttgcat ttgatcggca aaaacaggat
     1801 cagttgaggc aagcgaaatc gctgttggca agcctgccgc cagttctttg gcaaaagtcg
     1861 gaccggaaag caccgcaagc gggtaaattt cccccaaaat ttccttagct accgtttgca
     1921 acaaacgccc ggtatctcgc tccagccctt tggttgccca cattatacgg tgatcttggg
     1981 acaaaaaagg cttgatttga gccaatactt cagagaatac atgacttggc accacaatca
     2041 gcagatcttt cgatttttgt accgctgtag caagatctgc gacaatttct aaggcttccg
     2101 ggaatacaat atcaggcaga aaatcttggt tcattcgctc tgatgccaaa acggccattt
     2161 tgtcgggatt atgcccccat aaataggttt tgtgaccatt tcgagaaagg gcaatggcta
     2221 aagctgttcc ataagaacct gcacctaata ccgtgattgg ggcagaatag atttcactca
     2281 tatttcctct gttcggctta tttagaaaat tgcaaaaaat agtagaaaaa ataaccgctt
     2341 gtaaaaaaat aggtatgcct tgcataccta ttttctatct aaaatgctta gttcgctaaa
     2401 gccggctctt ctgcactttc ttcttggcgt tggagataat ccataaataa cgcatcaaag
     2461 tttaccggtg agagatttaa tgccgggaat gtaccacgat tcacaaggct tgagattagc
     2521 tcacgagcat aaggataaag cacattcgga cattgtgaag ctaagcagtg agcaagttga
     2581 atgccttcta aacctttaat agtaaacaca cctgcttgtt ttacttcaca aatgaaagcg
     2641 acatcattgc tgtcttctaa cgtagtttgc accgtaatgt gtaaggtaac ttcgtataaa
     2701 tcttcaccaa tttctttggt ttcagtatct aactcaaagc ctaattgtgg tttccactct
     2761 tgttggaaaa tagtcggtaa atttggtgct tcaaatgaaa catcttttac ataaatgcgt
     2821 tgaatttgaa gctcaaaagg ggtttgtgta gcttcttctg ttgatgcaac ttgattttct
     2881 tctgacataa aatttcctta ataatagatt atttatgttt tttaatagtt ggtaggcttg
     2941 ctgcacgcca gccgccaatc ccttctttta atacataaac tctgctaaag ccttgtttag
     3001 caagatctgc cgcaacacca cctgaagtga agtcattagt gtctactaca attaccgggc
     3061 gagctttgta tttatcaatc tgattgattt tagctgcttt aatgtctgac ggtaacacgt
     3121 gaatgctatc aataatatga ccggcacgga actcttcatc agaacgtaaa tcaaccacta
     3181 ctgcctcttc tttattaatc agctgtgtag cctgtgcgtt atcgacaatt ttatattttt
     3241 gcgttgcact tttataaaag ctaaaaatta ccgcaatcaa taatgcaacc caagcaaaca
     3301 ccatcaaggt atgattggca aaaaattgct ctaattgttg agcaaaagtt agttgttcca
     3361 taattcaatt tccttaacat caaaaaataa gcggtcgttt tgatgcaaat ttatcactaa
     3421 atttaaccgc ttgtaaaagt aataaaaggt gagcaaaagc ccaccttata aggaaattat
     3481 tgagcttgtt gtgacattac ttcgtcaaat gacattgttt tttcagtcac tttcgctttc
     3541 gctagcactg catcaaccgc ttgctcttct aataccacat tgcggatatt ttcagttaat
     3601 tgacggtttt tcgcatagtg agcaaccact tcagccggtt gttcgtaagc tgaagcgata
     3661 tctgcgatca tctcttcaac acgtttttca tctactttta attcgttaga accgatgatt
     3721 gaagataata ataagcccac ttgaacacgg cgtttagctt ctgcttcaaa taattcagcc
     3781 ggtaattgag ctgccatttc aggcttacca ccaaaacgtt gcaccgcttg ttggcgtaat
     3841 acgttgattt cttcttcaac cgcagcagcc ggcacttcaa catcattttg agcaattaag
     3901 ccgttgatta cttgagtttt cacacgtgaa gtgactgcat ttttaagttc acgttgcata
     3961 tttttcttaa tttctgcgcg taattcttcc actgattttg cattaccgaa ttttttcaca
     4021 aactcttcgg ttaattccgg taataccatc tcttctactt ttttcaaggt aattgcaaat
     4081 ttcgctgctt tgccttttaa gttttcagcg tggtattctt ctgggaaagt cacatcaatg
     4141 tcgaattgct cacccgcttt acgacctacg attccgtctt caaaccccgg gatcatacga
     4201 ccttgcccca taaataacac gaagtctgat gctttaccgc cttcgaattc ttcaccatct
     4261 actgaaccaa cgaaatcaat agttacacga gattcggctt ttgcagcctc ttggctttct
     4321 gaccaagtcg cttgttgttt gcgtaataca tctaccattt tgtctaaatc agcgtctgcg
     4381 atttcaacaa ccggtttttc tacttcgata ttctctaaac cttttaattc cacttctggg
     4441 aatacttcaa attccgcagt aaagctgaag tcttgacctg gttggtagtt gtttggcgtg
     4501 aaagtagggc gacctgccaa gttgattttt tcagcaatga cggcttcaaa gaatgcacgt
     4561 tgcatttcat cagataacac atcttgacgg gctgcaatac caaaacgttg ttcgataatt
     4621 gagtgtggca ctttaccttt acggaagcca tctacacgag catttttcgc atagcctttt
     4681 aattgttctt tgtatgctgc ttcgatttta tcagctggta cgttaatagt aacacggcgt
     4741 tttaagcctt ctaatgtttc aattgaaaat gacattctta aacctcggtt tagggatata
     4801 aaaaagatat acaaatagac ggttattgta gcgttctatc aagaaactgt cgagaagaaa
     4861 ctcattcgca aataaacagt tttgtaaaaa atccctaaaa tctgactgct tgtcaatggg
     4921 cttgcctaca agcggtcttt tttcaccgat ttttgcaaat gaaatgatat gatatggcta
     4981 gcttaatcgg cataaaagca aaagaggcat acctttcgat atgcctcttt ctagttttta
     5041 tttagctttt caatttatca aacaatctta attatttgat gattttagct acaacgcccg
     5101 cacctactgt acgaccacct tcacgaatcg caaaacgtaa accttcgtcc atcgcgattg
     5161 ggtgaattag gctaacagtc attttgatgt tgtcgcccgg cattaccatc tctacacctt
     5221 ccggtaattc gattgtacct gttacgtccg ttgtacggaa gtagaactgt ggacggtaac
     5281 ctttgaagaa tggagtgtga cgaccacctt cttcttttga taatacgtaa acttctgatt
     5341 cgaagtctgt gtgtggagtg attgaacccg gtttcgctaa tacttgacca cgttcgattt
     5401 cttcacgttt agtaccacgt aataatgcac ctacgttctc acccgcacga ccttcgtcta
     5461 ataatttacg gaacatctca acacctgtta cggttgtttt cgctgtatct ttgataccta
     5521 cgatttcaac ttcatcacca gtgcggatga taccacgctc aacacgacct gttactactg
     5581 taccacgacc tgaaattgag aatacgtctt cgattggtaa taagaatggt ttatcaatcg
     5641 cacgctcagg ctctggaatg tatgtgtcta agtggtttgc taattcaagg attttctctt
     5701 cccactctgc tacgccgttt aacgcttgta acgctgaacc acgtacgatt ggagtgtcat
     5761 cacctgggaa gtcgtattgt gaaagaagtt cacgcacttc catctcaact aattctaata
     5821 actcttcgtc atctaccatg tcgcatttgt ttaagaatac gatgatgtat ggtacaccta
     5881 cttggcgacc taataagatg tgctcacgag tttgtggcat tggaccgtca gttgctgcta
     5941 ctactaagat agcgccgtcc atttgtgccg caccggtaat catgttttta acatagtccg
     6001 cgtgtcccgg gcagtcaacg tgtgcgtagt gacgagttgc tgtatcgtac tcaacgtgtg
     6061 aagtgttgat ggtgatacca cgcgcttttt cttctggcgc gttatcgatt tggtcaaatg
     6121 cacgagctgc accaccgaag tgttttgcta atacggtagt gattgctgct gttaaagttg
     6181 ttttaccatg gtcaacgtgg ccgattgtac ccacattaac gtgcggtttt gtacgttcaa
     6241 atttttcttt agacacttta atatcttcct aaaaaatgag ccatagccct attgctatgg
     6301 ctcaggttaa acaaattatt ttctacgagc ttcaataatt gcatcagcaa cgtttttcgg
     6361 tgcttcagca tattttaaag gttccataga gtatgatgca cgaccttgag tttgtgaacg
     6421 taagtctgtt gcataaccaa acatttctga aagtggaact tcagcattga tcttaacaac
     6481 gaactcgttc gcttcttgac cgttaaccat tgcacgacga cggcttaagt caccgattac
     6541 atcacccacg taatccggtg gagtttcaac ttccactttc atgattggct caagtaatac
     6601 cgggcttgct tttgcgaacg ccgctttaaa tgctaatgat gcagcaagtt taaacgcaag
     6661 ttcagatgag tcaacatcgt ggtatgaacc gaagtgtaaa cgcacaccta aatctactac
     6721 cgggtaacct gctaacggac cagatttaag ctgttcttgg atacctttat caaccgcagg
     6781 aatgtattca ccagggatta caccaccttt gatctcgttt acaaactcat aacccggacc
     6841 ttcagcatct aatgggtata agtcgatcac aacgtgacca tattgaccac gaccaccaga
     6901 ttgttttgcg tgtttacctt caacatcatt aacacgagta cggatagttt cacggtaaga
     6961 tacttgtggt ttaccgatat ttgcttccac tttaaactca cgtttcatac ggtcaacgat
     7021 gatgtctaag tgtaactcac ccatacctga aatgatcgtt tcaccagatt cttcatctgt
     7081 gtgaacacgg aatgaagggt cttcttgtgc taaacgacct aaagctaagc ccattttctc
     7141 ttggtcagct ttggtttttg gttcaaccgc aacagagatt accggctcag ggaattccat
     7201 acgctcaaga atgattggtg catcgattgc acataatgtg tcacctgtac caacatcttt
     7261 taaaccgatt gctgcagcga tatcgcccgc acgaacttct ttgatctctt cacgtttgtt
     7321 tgcgtgcatc tgtacgatac gaccaaaacg ttcacgtttt tgttttactg agttgtaaac
     7381 tgtatcacca gagttaatta caccagagta aacacggaag aaggttaagt tacctacgaa
     7441 tgggtctgtt gcaattttga atgctaatga agcaaatggc tcatcgtcgc ttgcgtgacg
     7501 ctcaccttca gtttcatctt cattgatacc tttaattgca ggaatatcag ttggtgccgg
     7561 taagtaatcg attaccgcat caagcattgc ttgtacacct ttgtttttaa atgcagaacc
     7621 acagcaaaca gggattactt cacctgctaa tacacgttga cgtaatgctg ctttgatttc
     7681 atcttcagtt aattcttcac cagagaagaa tttttccatt aattcttcag aagcttcagc
     7741 tgctgcttca acaagcattg cacggtgttc ttcacaagct tcaagcatat ctgccggaat
     7801 atcttcataa gtgaaggtca taccttgatc agcttcgttc cagttgattg ctttcatttt
     7861 gattaaatca acaacacctt tgaagctgtc ttctgcaccg attggaagtt gtaatgcaac
     7921 tgagttacca cctaaacgcg ttttgatttg ctcaacaaca cgtaagaagt ttgcacctgt
     7981 acggtccatt ttgtttacga acgcgatacg tggcacttta tatttgttag cttgacgcca
     8041 tacagtttca gattgtggtt gaacaccacc aactgcacaa taaaccatca ccgcaccatc
     8101 aagtacacgc attgaacgtt ctacttcgat agtaaagtca acgtgtcccg gagtatcaat
     8161 aacgttgata cggtgttgtg gatattgttg tgacatacca gaccagaatg ctgttgttgc
     8221 agcagatgtg atcgtgatac cacgctcttg ttcttgttcc atccagtcca ttgttgcagc
     8281 accatcgtga acttcaccga ttttgtgact tacacctgta tagaacaaga tacgctctgt
     8341 agtagttgtt ttacccgcat cgatgtgcgc actgataccg atgttacggt aaagtgaaat
     8401 aggggttgta cgagccattt ttatttatta ccttgtttgg ggtttaaatt aaaattctta
     8461 ataagggtaa ggcttcaccg caaactgaga tgaagccttt gataagtttg cttaaacgat
     8521 taccaacgga agtgagcaaa cgctttgtta gcttcagcca tacggtgaac gtcttcacgt
     8581 ttcttcactg ctgaaccttt gttgtctgct gcatctgata attcgttagc taaacgtaat
     8641 gccattgatt tgtcaccacg tttacgagcg gcttctacga tccaacgcat tgctaaagca
     8701 ttacgacggg ttgggcgaac ttctacagga acttggtaag tagaaccacc cacacggcga
     8761 gatttaacct caacagttgg acgtacgttt tctaatgcga tttcgaaagc ttctaacgct
     8821 tctttaccag aacgttgtgt taaagtttct aatgcaccgt aaacgatttt ttctgcagta
     8881 gattttttac catctaccat taacacgtta ataaattttg caagtaattc tgaaccgaac
     8941 ttcggatctg gaagaatttt gcgtggttca atactacgac gacgtggcat tggaaatttc
     9001 tccgtatatt aaatcttcag gatatccaaa actcatctaa tctgccttaa ggcatcttga
     9061 ctggagttta tggtttaaat attaaaattt agacgtttgg ccttactcaa cggagaacca
     9121 ttaagcttta ggacgtttaa cgccgtattt agaacgacct tgtttacgat ctttaacgcc
     9181 tgcacagtca agtgcaccac gtacagtatg gtaacgaaca cccggtaagt ctttaacacg
     9241 accaccacgg attaatacaa cactgtgttc ttgaaggttg tgaccttcac caccgatgta
     9301 tgaagttact tcaaagccat ttgttaaacg aatacgacat actttacgta atgctgagtt
     9361 cggtttttta ggtgtagtag tgtatacacg agtacacacg ccacgtttct gcgggcaagc
     9421 ctctaatgca ggaacgttac tttttacaac ctttttcaca cgcggtttgc gtactagctg
     9481 gttgatagtt gccattaaaa aagctccagt taaaattatt aaaaataaaa ttaaaaacat
     9541 cctttcacac gaaaggacgg tgaattctaa attgaacgtg gataaaagtc aagcacagta
     9601 agagaacaac aatgatcatt tgttaatcaa tggaagtaat ttttctagca cttgtgaaga
     9661 ggtaatattt tccattttat ctgcttttag ataaaactga ttttgcccgt aagccccgat
     9721 aagttttgga tcggttgccc catataaaat cacatttggc ttatctaacg cagccgccaa
     9781 atggctcaag ccggtatcca ccgacaccac tgccgctgct cccgcaagtt gagtagcaat
     9841 atcggttaag ctcatttttg gcaatacctt agcattattc gccacactcg ctaaacgctc
     9901 cgcccgttct ttttcggctt ggtttcccca aggcaatctg actgccagat tgtgcttatc
     9961 caacgcttga atcaactccg cccaataggc ttcttgccaa tgtttatccg ctcgggtagt
    10021 ggcgtgaatc agcataatgt aagcggtcgt tttttgagga ttttttgcaa aatgttgtgc
    10081 aatgcaataa tcgccccgtt cggttggcaa agcataacct aatgattttg cacataattt
    10141 tctgatgcgt tctacagcgt gttgttggta ggggatatta aacgtttcat cgtaaaacag
    10201 actacttaac ccttctcggg cagagttttt atcatagcca aattttttgc ctttggcttg
    10261 gcgagttact aacaccgcac ttttaatcag cccttgagca tcaattaccg catcatactg
    10321 ctcacttttt agctgtttta aaaactgctg ccattctacc caagtctgtc tttggcaaag
    10381 gtttttccgc cagcggcgaa tagccaccgg aattacttta tttaccgctg aatgccacgc
    10441 aggaatttcg gtaaaattct cctccaccac ccaatctact tttaaatcgg gaatagcatt
    10501 ttgcatatcg gttatggctg gaagcgtatg taatacatct cccattgatg aggttttaac
    10561 gattaaaatt ttcatctttt ataaacaaac aagcggtctt tttttgcaaa aaattggcaa
    10621 attcaaccgc ttgttatcca tttaaactaa cagttcagct aatttgtcca tcaccatttc
    10681 aggctgaata tcaattaaac tttggtgata gccctctgcc ccttcgcctt tgcggatttt
    10741 aatcaaaccg ccttcaatta agcgcatgat ctcggcattt tgggataacg gcggcgtata
    10801 ttgtgggctg gtcggtccat ataaggcgac cagcggttta tttagtgctg cggcaatgtg
    10861 cattaagccg gagtcattgc tcacgactgc attacaatct gcaatcaaat ctaccgcttg
    10921 gtttaaatcg gtttgcccgg ctaaattcag gcaatagcgt tgtaaatttt caggtaatgc
    10981 caagcgaatt tgctcaccgg cggcttcatc ttttttcgag ccaaataacc gtaccgaata
    11041 gccttgctca atcaacattt ttgccaatat tgcataatga taatgcggcc aacgttttgc
    11101 cggtccaaat tcagcccctg gacaaaagcc gatggcagga cggttttccg cataagccaa
    11161 ctgtttttca aatttcgctt tggtctgctc aatttgttcg ctatcagttg tgaggtatgg
    11221 ataagcaatc ggcaactgtt gtgcggttgg aacagtagcc ttttcaaacg ccaaggctac
    11281 atagcgttgc accatcattg ggtagtcgtt tttattactg cgaagatcat ttaagaaaaa
    11341 atatctactt tcccctttcc aaccacgacg tctcgcaatt ttcgcaaaca gtggaataaa
    11401 ggcagacttt aatgaattag gcaatacaat cgccatatca tattgatttc tcagggattt
    11461 tccgagtcgg tagcgttcac acaaacgaaa tgaaccgtgt ccaatcggca ttgaaatcgc
    11521 attacgcacc tcaggcatac gggctaaaag cggacaacac caatcgggtg ccattacatc
    11581 aatttggcaa ttcggatact gtattttaag ctgttgataa agtgcgtgcg acatcatcat
    11641 atcacccacc caagacggtc ctataactaa aatattcatt tgcaaaattc tcttaaaaat
    11701 ttattcacaa tccttgtaag gattgttcac agcttcgctg ccgttggcaa agccaacgtt
    11761 caaaaagcga tactttttgt gtccgcttgt tactgcttta tgcaatttgc cgctaacgtt
    11821 tttttagtgt tgttatacaa aaatgcttta ccactacggg tttcatgcca agtccaacgg
    11881 atattagtgt attttttacc atctctcgtt acagaaggtg aaagctgatg cgttgtccct
    11941 tgataactca ctgtgactat ttcctttttt aacgcttttt ttgttcgtcc ctggcgttca
    12001 acttcaacct tcttatcatt ctcacaacga tataaactca ctttaggtgc ttgtgctatc
    12061 agtttagtca ctttctttac tgactctttt tccttttgta cttgatgaat ttgctcaaca
    12121 ggggtaacca ctgtactaca agcaactaac atcgctgagc ttagcgttaa gtagataagt
    12181 ttgtaagagg ggcaaaataa attcatcctt tatctccttt ttaaataaaa gagcaacgtt
    12241 tgtagcgttg ctctttttct tcattttatt tatttaaaag tatagctacc gtttgcttga
    12301 cgggtaaatt tgccgcctga gccaatgctc atttcaacca cacggttatt tttatctaaa
    12361 cgaacggtta ctcgctcgcc tacttttaaa ttactcacca cgttattgac tttgctcatc
    12421 gcattaacgt ctgagatatt taaattgtta tcacggaaca cttgcattag tgatacacct
    12481 tttggcacag tcattgtttt ggttgctacc gcatttgccg actcggcagc cggtgctgct
    12541 tttaccgctt cagtttttgt cggagctttc tcaactggtt tagctgctgt ttttgccgtt
    12601 tcttttgctg ccagtgtagc acgttcggtt ttcacttcag ttttagcagg ggctttaggc
    12661 tgttgtttta ccgttgtttc aggttggaac accacgctat tttccgtttt aggcttaatc
    12721 gccggttgtg caacaaccgg tttattttcc accggtttca ccgcaacagg ttgctcaact
    12781 actgcttgtg attgtgtttg cgactggcta tcttgtgacg gtttagccat tgtttgctcg
    12841 gtttgggctg gagcaactgt tacagactct tgttgagcgg cttgagcttg ttcttgagct
    12901 tttgcagctt cagcggcttc ttgttctgca cgagcttgtt cttccgcacg tttcgcttca
    12961 gcttcatcaa ccggtcggaa ttcaatcggc aagctaccac cttgctgaga ttgtagctct
    13021 tcgacagttt ccggcgtact tggttttaac aagaaaaaca gtaccaataa tgctaacgcc
    13081 aacaatgcca ctaataataa acggcgggtt ttaggtggca ttttagcttt aatctctttg
    13141 gttttagtgg ctgcagctgc cgcagtggct gctgcggcag ctgttgccgc atttggaata
    13201 atacgctcag tagtgaaact ctcgggtgca ggtttaacca cttctttttc cacattttct
    13261 tgtgcttcaa ccgcgttaaa agatgcggtt gatacacttt cttcaaccga atgaatggta
    13321 ttatccggct ctactgcttc tgtagcttct gaacttggag taaattggaa ttcttttgcc
    13381 tgcttagccg aaaagacatc agccgtatga tcttctactt tagcatcagg tcttttcata
    13441 attggggtaa acgtatgccc cggtgctttg gttgaacgca acgaaacata aggggcttga
    13501 gctgtatctg ttttggttgc ttcagcgtct tgagaggtaa ccgaagattc accaaaaacc
    13561 ggttctttgc gaaaattatt ttgtgtcata ttaattccaa ataatagcgt tgttaatgag
    13621 tataaaaatg gcgatattct aaagcaattc agccgctatt tgaatttatt taactaaatt
    13681 tacgcaacta gcggtcattt ttgcctaatt ttttgcaaat ctatttactt tcattgaatt
    13741 tcagaccaaa tgcaagtcta gaagtttagc cattttctat tttttcgatc tgtgtcgaaa
    13801 aatttatttt tgccggttga gttctatttt attccctctt aatcttcctt tcgacctttc
    13861 aacaactaat aaggaaatcc tatgaagaaa atcaaacaac ttggtctaac attcttgttt
    13921 ggcttaaatg caatggcttt tgcccaaaca ccgccaacta caatgcctgt tgaacaaaat
    13981 gcagtcactc aacccgtaca gcaaacacaa atcaccaacg ctaatgcggt aaatatcaat
    14041 actgctagtg ctgctgaatt gcaagacaag ctagtgggaa ttgggcagaa aaaagctcaa
    14101 gcgattgtcg attaccgtac taaaaacggc aattttaaaa acattgagca acttgctgaa
    14161 gtctccggaa ttgggcaagc aacggtagaa aaaaatcgag ccaatattat tttagaataa
    14221 gacaaccaaa ttgttgaagt ggaattccta caaagcccct atctgttttt ttctttgtag
    14281 ccacttcaac accttaaaga ttttttatct attttgcatt gtatttgcta tacttcgccc
    14341 ccaattaaac gcaaattctc gtttttaaat aaaaggaaaa cgcaatgcaa ttaaaaaaat
    14401 atccagttcc aacctatttc caacgtgtga aaatcgctct gcactattta ctgccacaac
    14461 tggcaattac ccgtgcggca ggttggttag cagagcaaaa atggggcgta gttactcacc
    14521 ttatcatcaa actgtttgca aaacagtata aagtcaattt atctgaagct aaaaaaaccg
    14581 agccgtcaga ttatgctact tttaatgaat tttttatccg tgaattaaaa gaaaacgcac
    14641 gcccgattaa tcaagatcca aatacacttt gcttaccggc agaaggtaaa gtcagcgaat
    14701 caggcgaaat ttcggataac cgtttattac aagcaaaagg gcatcatttt acgctagaaa
    14761 ccttattggc aaatgatgaa gaaatggcgg caaaatttaa agacggcaca tttattacca
    14821 cctacttatc gccaactgat taccatcgtg tacatatgcc gtgcgatgga acattgcgta
    14881 aaatgattta tgtgccgggc gaattgttct ccgtcaatcc gtttttagcc gaacacgttc
    14941 caaacttatt cgctcgcaat gagcgtgtga tttgtgaatt tgacaccgca ttcggaccaa
    15001 tgctacagat tttggtcggt gcaacggtta ccgcaagtat gagtacggtt tgggctggcg
    15061 taattaatcc gcctcgcgct gatgaagtgg tagtttggaa ttacgaaacc gaaggcgaaa
    15121 aagccatcaa actccaaaaa ggcgaagaaa tgggagcttt ccgtttaggc tcaaccgtaa
    15181 ttaacctctt cccgaaaggt aaagtacatc tcaaccctgc acttattgca ggaatgcaaa
    15241 cccgaatggg ggaagagttg ggtagaatta tcaattaaat cctgagttaa ttttacctaa
    15301 actgtttgct ataatcttcc gataagaaac tcttatcgga aggaaaaata tgtacacact
    15361 tcaagaatta aagcagatga acgagcaaga aattcgctct cgattaatct ctcctgcaat
    15421 tagaaatgca ggatggtcgg atagacaaat tggcgaagag tacacattta aaaccaataa
    15481 acgatttaca gatgggcaag tagttgttga tccaaaaacg acgcaaacta aacgcatcga
    15541 agcaaaacgg gttgattatc tgctctatac ttccgcaaat caaaaaattg ccgtggtcga
    15601 ggccaaagat aaccatcact cttcccgaca cggcttacag caagcaatga catacgctcg
    15661 attgcttgat gtcccttttg catatagctc aaatggtgat gagtttgtgg aacacgattt
    15721 tatcactggc gtacaacgaa cccttccgat gtcagcattt cctactcccg acgaactcca
    15781 ccaacgttgg gcgaaacaat ataccaccga agagctgaaa attattcaac agccttatta
    15841 caccagtagc actgacacct ttaaagagcc tcgttactac caagaaattg cgattaaccg
    15901 tactgttgaa gctgtagcaa aaggtcagca gaggatttta ttggtaatgg caacaggcac
    15961 gggtaaaacc tatacggctt tccaaattat ccatcgttta aaaaccagtg gtgcgaaaaa
    16021 gaaaatttta tttcttgccg atcgcaatgt actagtcgat cagccaatga tgcaagactt
    16081 caaaccgttt aaagacaaaa tggtgaaagt cagcaataaa aagctcgaca gtgcttatga
    16141 agtctatctt gcactttatc aacagcttga cggtgaaacc gataacgaac tgtttaagca
    16201 gttccaaccc tctttctttg atcttattgt gatcgatgaa tgtcatcgtg gttcagcaaa
    16261 agaagattct gactggcgtg ctattttgaa gtatttcaag gacgccaccc atatcggtat
    16321 gactgcaacc cccaaagaaa ccgatgatgc cagcaacatt acctattttg gcgagccgat
    16381 ttatacctat agcctcaaac aaggtattga agacggcttt cttgcccctt atcgagtgat
    16441 tcgtgttgat ctggactgtg atttaaaagg ctggcgacca accaaaggtt taaaagatca
    16501 tcaaggtaat gagattgaag atcgcctcta tacagtcaaa gatttcgatt acaacattgt
    16561 gctaaatgat cgcacccgct tggttgcgga gagaatttgc gaatttttac gcaaaaccga
    16621 ccgcttgcag aaaacaatag tcttctgtgc caacatcaac catgcagagc gaatggtggc
    16681 agagctttca cgcctcaatg ccgatatgtt gaaacacaat tccaaatatg tctgccaaat
    16741 tacaggcgac caatcaggca aaggcgttga tttagaagaa ttttgtgatg tagcaaatga
    16801 agggaaagat taccctgtga ttgccgtaac ctctaaattg atgacaacag gtgtagatag
    16861 caaaacctgt aagctggttg tacttgatgc aaacattcaa tcaatgacgg aatttaaaca
    16921 aattatcgga cgaggcactc gtcttcgccc tgatttcggc aaagaatttt tcaccatttt
    16981 agattttcga ggcgttactc gcctgtttgc tgatccggca tttgatggtg agccggtggt
    17041 gattttagat gtaccggaaa ataagccact tgtcgaacaa ccgcttccta aacccagtga
    17101 taaggacatc attaaagacc ccgagctaaa gccatataaa atcccggtag ttgtcgattt
    17161 cggcacactc aaattacgca ttttagacga gaaagtccaa tatctgaatg agcagggcga
    17221 gctggtttct gaagatattg atctgttctc aaaacgggaa atgcaaaaag agttcggcag
    17281 ttacgaacag ttccaacagc aatggacaac aagcggtgaa attttctcga aattttacac
    17341 cgatccaaaa tggctggcag cccttcgcca aacacgccaa ttcagccacg atgttgaaga
    17401 ctttgatgta ttaagccaca tctcctttgg caaaaaacca ctgactaaaa ccgaacgagc
    17461 agaaaaagtg aaacaaagtg gctatgtgga gcagtattcg cccgaaaacc agcaagtttt
    17521 agggttgctg cttaatgaat atgtcaaatc cggcaatcaa gatctgacta aaaccgaaat
    17581 tttaaagaca ccgcaattca gccgttttgg aggtctagtg tctattatta aaaatttcgg
    17641 cggtaaagaa ctctaccaga aagcggtaaa aggcttagaa aagatgattt attcggaata
    17701 acaagcggtt attttacaac aaaattttac agataaacaa ggatcacaat gagcctcaac
    17761 aacatagtaa aacgcctgca agatgtaatg cgtaacgatg cagggattaa cggtgatgcc
    17821 caacgcattg aacagatcgt ctggatctta ttcttgaaaa tttacgatgc taaagaacaa
    17881 gagtgggaac tggaaaacga tgaatatcac tcaattttgc cgaatttttt acgctggcaa
    17941 aattgggcgg aagataaaaa agacggcaaa gcgatgactg gtgatgaatt actctctttc
    18001 gtcaataacg aacttttccc gactttaaaa aatctgccta tttcagccga tacgccgatg
    18061 aaccaacgga ttatccgtgc tgcttttgaa gataataaca actatatgaa aaatggggtg
    18121 ttattacgtc aggttatcaa tatcattgat gaaattgatt ttgcccatta ccaagaacga
    18181 cacgcctttg gcgatattta tgaaaatatc ctcaaaagtt tacaaagtgc aggtaatgca
    18241 ggggaatttt atacccctcg cgctgtaacc gattttatgg tacaagcaat caaaccgaaa
    18301 cttggcgaac gcattgccga ttttgcctgt gggacgggtg gttttttaac ttcggcgtta
    18361 aaggtattgg aaagtcaaat tcaaaccctt tctgaccgca ctttattcaa taattctgtg
    18421 tatggcattg agaaaaaagc ccttcctcac ctgctttgta ttaccaattt attgttgcac
    18481 gatattgata atccgaatgt acatcacgat aatgctctag aaaaatcggt caaagattac
    18541 accgaaaatg ataaatttga tgtgattttg atgaatccac cttacggagg ttcagaaatt
    18601 gagcaaatta aaaagaattt ccctacggct ttacagagca gtgaaaccgc tgatctgttt
    18661 atgtcggtaa ttatgtaccg cctaaaacag aatggacggg ctgcggtggt gctgcctgac
    18721 ggatttttgt tcggcacaga taatgccaaa gttgcgatta agaaaaagct attagaagag
    18781 tttaatctgc atacgattat ccgcttaccg catagtgtct ttgcccctta tacctcgatt
    18841 acgactaata ttctgttttt tgataaaacc gagccaaccc gtgaaacttg ggtttatcgc
    18901 cttgatatgc cacagggtta taaaaatttc tccaaaacta aaccgatgaa attggaacat
    18961 ttcaacgaag ttgttgaatg gtggggaaat cgccaagtga ttgaagtcga agggtttgac
    19021 aaagcgaaaa gttttacatt aaaggaaatg tccgaccgtc aatacaacct cgatttatgt
    19081 ggctttcctc acgaagaaga ggaaatcctt ccgcctgatg aactaattgc agattatcag
    19141 cgaaaacgca ccgcttacaa tgctgatatt gataagattc tagcagaaat taccgatatt
    19201 ttaggcatta aactgtaata ggagcataaa atgctgactt gggaacatat cgaaaaaatg
    19261 attgcacatc agcaatttat ccgagaaaag gcagaaattg aatataaaga ggcaaaaaat
    19321 agtgtgccaa aagatctttg gcatacttat tcggcttttg caaacagcaa tggtggcttt
    19381 attttattag gaataaagga aaatgaacaa ggacattttc ctgaactgct aatttctggt
    19441 gttaatgatc ctgataaagt cattgatgaa ttattttccc aatctcgtag tgaaaaagtc
    19501 agtaaatcct tattaaagga taatgatgtg aaaatctttc aagttgaaaa taaaagcgtg
    19561 attgcaattt atgtgaaagc agctgatatt ttagaccgtc ctatccattt aaatggtgat
    19621 tatcgacttt cttatgttcg tttaaattca ggcgatcata aactcacctc ctctgaactg
    19681 cgttctttca tttcaagcta tagccaaaaa gatgctgatg ggagaattgt gccttatgct
    19741 tctattgatg atatcagttt aagcacattg gataaataca gaaatctgct gaaagcccat
    19801 aacagcacat caaaattact tacgttatcc gatgagaaat tgctgcaaga aattggggct
    19861 tataaaaaag atttagcaca gaatagcgag ggcattacca atgcaggctt attgatgttt
    19921 ggtaagcact atgccattac aagcatattt ccccacttct tttttgaata ttacgaagaa
    19981 gctggtgatg taaaacgcta tacattacgg attaccgatt ttgatcttga agaaggcaat
    20041 ctctttgaat tttacttgaa aattgtgcca cttattcaag agcttggtaa agataaacat
    20101 ttcaaattag ataatttaac ccgtactgag gaaaatgaaa ttactgacgg gctacgggaa
    20161 gctctaatca atgcaattac tcacgcagat tactttaaca atgttcgcca tctaaaagtc
    20221 attaaaacca aagataacaa actgattttt gaaaatgcag gagtaatgtt ggttgatatt
    20281 cccacggcta ttgcagggca aagatcggaa tgccgtaatt ctattattca taacttattc
    20341 caccgtttag ggctatgtga gcgacaagga caaggcgttc gggatattta ttcatattgg
    20401 caaagccgaa atttcaattc accgctttta gaaagtgaaa ttgattacac taaactcacc
    20461 ttaacttttc aatcaggtat gattgcagat tttgaggaaa aatttaccgc ttgctttggc
    20521 gaaaatttct ctgagttaca gcctattcaa aaagattgct tactctttat cgccttcaat
    20581 caaggtaaag caatgtttgc agaattagca caaaaactgc cccaatatcc acgcagaaat
    20641 attactttgg cactagcacg actgaaagaa aaaggcttac taaccagttc ggggagtccg
    20701 agaaaacaac tctataccct agtgaattta gaatggataa tcacagagaa atcacaagat
    20761 gattatttta atccaccttt cgaccaactt gataaagcta gtgagagtaa gggcgccccc
    20821 ccgaagaaaa gcaaagtgaa gcaagtcaaa caattacccc agaacaaagc aaacaaatta
    20881 cccccgaagt aaatcgctta ctttcagtgt tgcttgagcc gatgaagagc aaagaaatta
    20941 tgaaaaaact caggttaaaa gatgataagc atttccgtat gcagtattta aaacctgcac
    21001 taacgcttgg tgtgatcgaa atgcaattcc cagaaaaccc aaaacaccgt aatcaaagat
    21061 actttaagaa ataacaaccg attattgaga ataaaaaatg aaagcccaac agctaaaaaa
    21121 cgcaatccta caactagcaa tccaaggcaa gctcgttcca caagatccaa atgatgaacc
    21181 ggcaagcgaa ctcctttgca aaatccaagc agaaaaagac cgcttgattg cagaaggaaa
    21241 gatcaaaaaa aacaaaaaaa ctgccgataa agccccttat accattgaac caccttttga
    21301 gattcctgag agttgggtgt gggtgaggtt gggggatata tgcctaaaaa ttacagatgg
    21361 aactcatcat tctcccccaa atatcgataa gagtgatttt ctttatatta cagctaaaaa
    21421 tataaagaaa gatggattag atttatcaaa aattagctat gtaacaaaag aaattcataa
    21481 tgaaattttc agccgttgta
//
LOCUS       DANLYI010000015        21260 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.015, whole genome shotgun sequence.
ACCESSION   DANLYI010000015 DANLYI010000000
VERSION     DANLYI010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 21260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21260
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.015"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            230..526
                     /locus_tag="PXD81_000461"
     CDS             230..526
                     /locus_tag="PXD81_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HDL5536525.1"
                     /translation="MTAITIPQFEEVTSKQFQSEFGRIVDIVKGKTPVAITQYGRPTM
                     VLFPYDQAVEARRLLAKQRLLEMLAERDKNPPAALAEITLEELDKMIDEERNAL"
     gene            516..932
                     /locus_tag="PXD81_000462"
     CDS             516..932
                     /locus_tag="PXD81_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative toxin-antitoxin system toxin component,
                     PIN family"
                     /protein_id="HDL5536526.1"
                     /translation="MHSDYLVIDTNILISGLLFPKSNPNLAVKKALLGYTICVSQATF
                     DEFLEVSARPKFAKYFSIQEREEFIANLLDAVKFVEIKETITDCQDPKDNKFLEVAIS
                     AKAKYLVTGDKKDLLSMNPYRNVEIISVSEFLAETR"
     gene            932..2590
                     /locus_tag="PXD81_000463"
     CDS             932..2590
                     /locus_tag="PXD81_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhnD/SsuA/transferrin family substrate-binding
                     protein"
                     /protein_id="HDL5536527.1"
                     /translation="MKRFLTLFLLLFSSFSLAETHQIGILAQRGEAYTRTHWQPWVDW
                     LNSQFPNERFELVPLGINEASRRAELDFLLTNQAQFFYLSNQNVRWLATLNSPFSANG
                     EQGAVGSSIWVRADSHYQQLSDLKNQTISAVDNDAFGGFLLGLYQFHQAGMRQNRDFS
                     VQFSGFPVENALALLAEKQVEAAIVPVCLLEELEKEGKFKRSDFRLILQNPQAQGCLA
                     STPLLPNWSLAAMENVPNELAVQLATRLLNSHNPDLPRWTLPFSSAQADHILRELYRH
                     PQQKSLWATVLDWVRLNKFGLLAVVLFILLNLVALRYQVYRKSKALQQAHCKMQQYQQ
                     ELTRADRLALLGEMTTGFAHELKQPLSAVRMYAEGLKSQNSDPYQQRILDKLIAQVDR
                     AVKTMQSIRDWAQNRPSGEPQAVILNQLITNVIEFVAVENRQNAQISLIADRTFRLNL
                     HATVLEQVLTNCLLNALQAGASEISVRLQAVENGLEIAIEDNGGGFSPAQLEFPFVPF
                     RTDKPHGLGLGLVLCQRLMQSLNGRIVLANGENGAKVVLLLPQI"
     gene            2676..3323
                     /locus_tag="PXD81_000464"
     CDS             2676..3323
                     /locus_tag="PXD81_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL5536528.1"
                     /translation="MIIHILDDEESILDAMSFLLAPLGVEIQTWQSSLDFLAQADLHR
                     QGVLLLDIRMPLPDGQQVHQQLREAQSTLAVVIMTAHGDVPMAVAELKKGAVDFLQKP
                     ASFEQLKQAIAQAEKVSEKAVKIREISQNYAKLTEKERNLVPLIMQGFTNKQIADKLA
                     ISVRTVEVHRANVMEKMQAESLAGLVQMIGELQYKGSGNCSATFSFDFCRINLLL"
     gene            complement(3307..4245)
                     /gene="ttcA"
                     /locus_tag="PXD81_000465"
     CDS             complement(3307..4245)
                     /gene="ttcA"
                     /locus_tag="PXD81_000465"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005634611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HDL5536529.1"
                     /translation="MTDDIQKSDKKITYNFNKLQKRLRRNVGNAIADFGMIEEGDRVM
                     VCLSGGKDSYTLLDILLNLQQSAPVNFSVVAVNLDQKQPGFPEEVLPRYLESIGVEYK
                     IVEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGATKIALGHHRDDMLATLF
                     LNMFYGGKMKSMPPKLISDDGKQIVIRPLAYCKEKDIIKYAEAKQFPIIPCNLCGSQP
                     NLQRQVVKEMLNTWDRQYPGRLETMFSALQDIVPSHLCDPKLFDFKGIKHGQTIEGIE
                     GDTAFDDVKIEAKTFLYEDDDNNFAEAGMIQFKEVN"
     gene            complement(4308..5021)
                     /gene="ubiG"
                     /locus_tag="PXD81_000466"
     CDS             complement(4308..5021)
                     /gene="ubiG"
                     /locus_tag="PXD81_000466"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014667941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HDL5536530.1"
                     /translation="MQNVDQQEITKFENMAKTWWDPNGSFKPIHLLNPLRLAYILEKS
                     NGLFGKKVLDVGCGGGILSEAMAKQGAIVTGIDMTSQPLEVAKQHAKESGLEIDYQQT
                     TIENFLAKMTACNQEKFDVITCMEMLEHVPDPLSIIQSCKALLKPDGVLFFSTINRTF
                     KAYMLVIIGAEYVLKMLPKGTHEFEKFIKPAELLAWCDQADLRCQEMRGYHFNPLTEK
                     FWLNNDVSCNYIATLKPNN"
     gene            5086..5466
                     /locus_tag="PXD81_000467"
     CDS             5086..5466
                     /locus_tag="PXD81_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine dimer DNA glycosylase/endonuclease V"
                     /protein_id="HDL5536531.1"
                     /translation="MTRINLVPPTELCDQHLLAEHRELTRIPNAVAKGKFSLKGQPED
                     YKLGEGHVRFFFNKLTFLKKRYDALHEECLARGFKVQYFWAEHLPQDPSLWLDYQPTE
                     TALTVNRERIALRMPANPRFSERK"
     gene            5563..8259
                     /gene="gyrA"
                     /locus_tag="PXD81_000468"
     CDS             5563..8259
                     /gene="gyrA"
                     /locus_tag="PXD81_000468"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015587009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HDL5536532.1"
                     /translation="MSELAKDIIPVSIEDELKTSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLFSMDQNSNTYNKPHVKSARVVGDVIGKYHPHGDSAVYDTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDAPAAMRYTEVRMQKITQELLTDLDKETVDFSPNYDGKEMIPDVLP
                     TKIPALLVNGSSGIAVGMATNIPPHNLGEVLDGCLAYIENEQISVEELMRYIPGPDFP
                     TGAIINGRKGIEEAYRTGRGKVYVRAKASVETTDKGREQIVVTELPYQVNKAKLIEKI
                     AELVKEKKIEGISKIDDYSDKKGISIVIEVKRDAVGEVVLNHLYSLTQMQVTFGINMV
                     ALDNGQPKVLNLRQIIEAFVKHRREVVTRRTIFELRKARERAHILEGLAIALANIDPV
                     IELIRASKTAEEAREGLLARPWALGNVAPMLEAAGVGASRPEDLPEHLGVRDGEYYLS
                     EAQARAILELRLHRLTGLEHEKIVEEYKELLTEIGELIHILTSPERLMEVIREELERV
                     KTDFNDERRTEITAASGDINIEDLIAQEDVVVTLSHAGYVKYQPLSDYEAQRRGGKGK
                     SATKMKEDDFIEKLLVANTHDTILCFSSRGRLYQLKVYQLPQASRGARGTPIVNILPL
                     VKEENERITAILPIPNGEFSADKFIFMATASGVVKKVSLDAFSNVRASGLIALKLREG
                     DELIGVDITDGNSEIMLFSAQGRVVRFAETAVRAMGRTATGVRGIKLSTTEISSEEIE
                     EAEIIETDNEEAEDSSSVSLDKDRVVSLVIPRTEGDILTVTQNGYGKRTVISEYPVKS
                     RATKGVVSIKVNERNGKVVAAVQVEETDQIMLITDAGTLVRTRVAEVSLVGRNTQGVR
                     IIRTAEDEQVVSLERVCEPEDDENESLDALENAETAETSTEE"
     gene            8354..10318
                     /locus_tag="PXD81_000469"
     CDS             8354..10318
                     /locus_tag="PXD81_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005600393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL5536533.1"
                     /translation="MALINLSNAYLGFGDHPLLDHTELHIEPGERVCLVGRNGAGKST
                     LMKILAGEVQLDDGKLVFEKDIVVTRLEQDPPRHIEETVFDYVAEGIAHLADLLKQYH
                     HISLQMQTDYSDELLAKLSAVQSQLEHANGWQFENRIQDTLKLLELAPDKRLCELSGG
                     WVRRAALARALVADPDVLLLDEPTNHLDIEAIAWLENLLLDFKGSIIFISHDRAFIRK
                     MATRIVDLDRGKLVSYPSNYDKYLEEKAEDLRVEALQNELFDKKLAQEEVWIRQGIKA
                     RRTRNEGRVRALKALREERRNRREVQGTAKIQIDNTARSGKIVFEVENAGYEIGGRTL
                     LKNFSTTILRGDKIALIGANGIGKTTLIKLLLGEIQPTSGSVRCGTKLEVAYFDQYRV
                     DLDPEKTVMDNVADGKQDVEVNGVKRHVLGYLQDFLFPPKRAMTPVKALSGGERNCLL
                     LAKLLLKPNNLLILDEPTNDLDVETLELLEEMLTDYQGTLIIVSHDRQFVDNTVTECY
                     FFEGEGVVNKYVGGYFDAKQQQENYFAATADKRLNSSKNLQKIEEKAPLAEQEKQKAD
                     QEKTKAEPTKKTKLSYKEQRELDELPAKMEQLEADIEALQAEVNSAEFFAKDPSYTNA
                     QLQKLADTEAALEQAFERWEALENIKNGNI"
     gene            10400..11158
                     /gene="tcdA"
                     /locus_tag="PXD81_000470"
     CDS             10400..11158
                     /gene="tcdA"
                     /locus_tag="PXD81_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HDL5536534.1"
                     /translation="MTERLDNYEQRFGGIGRLYTPEGLEKLRRSHICVIGIGGVGSWA
                     VEALARSGIGKITMIDMDDICVTNINRQIHAMSGTVAQLKTEAMKERIERINPECVVE
                     IIDDFLTQDNLADYLNRGYDYVIDAIDSLRVKAAMIAFCKRNKIKIITTGGAGGQTDP
                     TKIQITDLSKTVQDPLAARVRSLLRKEYGFSNKNKFGVECVSSTQQLIFPQMGEACEV
                     SATMNCANGFGAVTMVTATFGFFAVSRVIDKLLK"
     gene            11205..12572
                     /locus_tag="PXD81_000471"
     CDS             11205..12572
                     /locus_tag="PXD81_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LOG family protein"
                     /protein_id="HDL5536535.1"
                     /translation="MAIHRVNPKGSMEQLSHLEMELLAKNTQGNLHQLYRNCSLAVLN
                     SGVHTDDSRALLSQYPDFEIRLLTREKGVSLELHNPPETAFVDGKMILNIQYHLFAVL
                     RDIVFVNALKNAIRPLEETSLEALTTNTVFSILRNAKAIEMNTDPNLVVCWGGHSINE
                     TEYQYCRAVGQEFGLRELNIVTGCGAGVMEAPMKGAAIGHANQRYKNGRFIGITEPSI
                     IASEPPNPIVNELVIMPDIEKRLEAFVRLGHAIVVFPGGPGTLEELLYIIGIKLNPAN
                     HAQQLPLILTAPKESADYFYAIDQFIGETLGEEARSLYEIIIDDSVLVARKVKQAMIE
                     VKSSRYESGDAYNFNWSLKIEEDFITPFIPTHENMANLNLSLAQPKEQLAANLRRAFS
                     GIVAGNIKPETSDLIEKYGVFKLHGDRKLMEHIDKLLERFVVQHRMKIPTGEAYQPCY
                     EIVKG"
     gene            12592..14139
                     /locus_tag="PXD81_000472"
     CDS             12592..14139
                     /locus_tag="PXD81_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HDL5536536.1"
                     /translation="MYLKHIEISGFRGINHLSLQLRPNMVLIGENAWGKSSLIDALSL
                     IFNLEQALYQFQFKDFHIQYTDTPNQVRDIQLVFTFCEEHQHDLAVQFPAYLAQAAFY
                     IDNHRCVSLAVTGKLHGEQISTDYQFVDQSLKPIQIEQAENAIRQFIYHHPVYRLRDA
                     RLNNRVHNELLLKGSSQLEDDFQRELNALSSLLKYYFLTSQSRYLIKTSMQDTALWWE
                     KVKSLCLRLKQQPELVESAQQHISTLFLFNPRSTVIEKPIILFEDLNAQLHPRMIAIF
                     WELLGFLPSQRITTTNSIELLSQVPLREICRLVRHHDHTQAYWLERHSLGKEDLRKLT
                     FHIHHNRGLALFAKAWILVEGETEVWILQELARLLDIHLEMEGIKIVEFAQCGLRPLI
                     KYARAMGIEWYVLTDGDDSGKRYADTVKSMEEDFAHRLTMLPQKDIEHFFYKAGLRNV
                     FIQLANWRSQEKYPVSYIIKRAIQRSSKPDLAIALANEMGQRGEHSIPTLFKQLFADV
                     LALINKV"
     gene            14362..15396
                     /gene="metN"
                     /locus_tag="PXD81_000473"
     CDS             14362..15396
                     /gene="metN"
                     /locus_tag="PXD81_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HDL5536537.1"
                     /translation="MIQLKNISKQFEVKGKKITALDNVNLEVPRGTIYGVIGASGAGK
                     STLIRCVNLLERPTIGQVIVDGQDLTAMSDKELIHARRNIAMIFQHFNLLSSQTVFEN
                     VALSLRLSNTPKEQVEKKVNELLDLVGLTDRKDVYPANLSGGQKQRVAIARALASDPH
                     VLLCDEATSALDPATTQSILQLLKDINKRLGLTILLITHEMEVVKRICDRVAVIDKGQ
                     LIESGSVSEIFSNPKTELAQKFIQSTFHFELPQEYTEQLSSSPTEHSKPIIKFEFTGR
                     SVDAPLLSMASKKFGIDFSILMSQIDYAGGVKFGFVIAEVSGNPDSIAEAKFYLIDNN
                     VKVEVLGYVG"
     gene            15386..16063
                     /locus_tag="PXD81_000474"
     CDS             15386..16063
                     /locus_tag="PXD81_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005597560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL5536538.1"
                     /translation="MWADFMQEMTPQMWSLVWESTFETVYMGFVATALAVVIGLPIGF
                     LAFLTGKGEILENPRLHQVLDVVINIGRSIPFIILLIILLPFTRLVVGTTLGTTAAIV
                     PLSVSAIPFFARLTSNALLEIPSGLTEAAKSMGATNWQVVTKFYLPESLPILINGITL
                     TLVALIGYSAMAGAVGGGGLGNLAISYGEHRNMVYVKWIATIIIVAIVMISQKLGDHL
                     AKRYDHR"
     gene            16086..16919
                     /locus_tag="PXD81_000475"
     CDS             16086..16919
                     /locus_tag="PXD81_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009874634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family lipoprotein"
                     /protein_id="HDL5536539.1"
                     /translation="MSFKKILGVALVSALALTACKEEKKAESTAAPAAQAPAKIKVGV
                     MSGPEHTVAERAAQIAKEKYGLEVEFVLFNDYALPNTAVSKGDLDANAFQHKPYLDKD
                     SQSKGLNNLVIVGNTFVYPLAGYSKKVKNVSELAEGAVIAVPNDPSNLARALILLEKQ
                     GLIKLKDNTNLFSTSVDIIENPKNLKIKEVDTSIAAKALDDVDLAVVNNTYAGQVGLN
                     TQDHGVFVESKDSPYVNIIVARQDNKDAANVQNFIKSYQTEEVYQEAQKHFKDGVVKG
                     W"
     gene            17002..17832
                     /locus_tag="PXD81_000476"
     CDS             17002..17832
                     /locus_tag="PXD81_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family lipoprotein"
                     /protein_id="HDL5536540.1"
                     /translation="MNFKKLLGVALVSALALTACKDEKAQAPATTAKTENKAPLKVGV
                     MTGPEAQMTEVAVKIAKEKYGLDVELVQFTEYTQPNAALHSKDLDANAFQTVPYLEQE
                     VKDRGYKLAIIGNTLVWPIAAYSKKIKNISELKDGATVAIPNNASNTARALLLLQAHG
                     LLKLKDPKNVFATENDIIENPKNIKIVQADTSLLTRMLDDVELAVINNTYAGQAGLSP
                     DKDGIIVESKDSPYVNLVVSREDNKDDPRLQTFVKSFQTEEVFQEALKLFNGGVVKGW
                     "
     gene            17884..18675
                     /gene="metQ"
                     /locus_tag="PXD81_000477"
     CDS             17884..18675
                     /gene="metQ"
                     /locus_tag="PXD81_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HDL5536541.1"
                     /translation="MKIMKLAGAVAIFSLFLTACNDKAEKLKVGVISGPEHKVMEVAA
                     KIAKEKYNRDVELVVFTDYATPNAALDKGDLDLNAFQHKPYLDNQIQEKGYKLVPVGN
                     TFVYPIAAYSKKIKSLAELKDGDTIAVPNDPTNLARALILLEKQDLIKLRADAGLKAT
                     SVDIIENPRKLVIQEIEAPLLPRTLDDVAFSIINTTYAGQNGLTPTKDGIFVEDKDSP
                     YVNLIVARENNQHSEAVKDLVKAYQTEEVYNKANEEFKGAMIKGW"
     gene            complement(18720..19871)
                     /gene="metK"
                     /locus_tag="PXD81_000478"
     CDS             complement(18720..19871)
                     /gene="metK"
                     /locus_tag="PXD81_000478"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018356717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HDL5536542.1"
                     /translation="MAINLFTSESVSEGHPDKIADQISDAVLDEILKQDPKARVACET
                     YVKTGMALVGGEITTSAWVDIENLTRQVICDIGYTHSDMGFDAHSCAVLNAIGKQSPD
                     INQGVDRADPLEQGAGDQGIMFGYATNETEVLMPAPITYAHRLMEQQSKVRKSGKLDW
                     LRPDAKSQLTFAYENNKIIGIDAVVLSTQHAEHISQKELIEGVMEEIIKPVLPSEWLS
                     QNTKYFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGAARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQLSYAIGVADPTSIMVETFGTGKVSNEILVKLIYQNF
                     DLRPYGLIKMLDLIKPIYRETAAYGHFGRKHFPWEKTDKAEALRAGAGL"
     gene            complement(20054..20896)
                     /locus_tag="PXD81_000479"
     CDS             complement(20054..20896)
                     /locus_tag="PXD81_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005618427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HDL5536543.1"
                     /translation="MKVGLVLEGGGLRGIFTAGVLDVFLDEHIHIDGAIGVSAGALFG
                     VNLPSKQKGRALRYNKKYAKDKRYMGLHSLLTTGNIVNRDFAYYELPSKLDPFDQQAF
                     EDSGMDFWVTVTNVETGEPEYIKIDNVFEQMEAFRATSSMPIVSKIVEIEGKKYLDGG
                     VSDSIPVQKFIEMGYDKIIVVLTRPLEYRKKPSSMWLFKRFYKKYPKLVQRWENRYAE
                     YNQAVEQIIQLNEKQQIFVIRPSRTVKISRLETDVNKIQAMYDLGVEDAKNALAGLRA
                     YLAK"
     gene            20956..>21260
                     /locus_tag="PXD81_000480"
     CDS             20956..>21260
                     /locus_tag="PXD81_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HDL5536544.1"
                     /translation="MADAEFMDFLLDQLSPISGLRSKKMFGEYCLFFDNKIVAIISKD
                     YRIFVKANPQTLPLFLAENAEQFHYFAKGKVQKMHYWSIPELAIEEADELVKWIRLG"
BASE COUNT         6232 a         4270 c         4932 g         5826 t
ORIGIN      
        1 tcttttgttc aaggcaagta tggatacctg accgcaggcc tggagataga acctacatat
       61 aatatgctct actacattaa gccataaaaa atagaaaatc aagtgtttgt tcaagttttt
      121 atgttattga atttacaata actacaaaaa tttatcttaa ttggctcgat tgatttttac
      181 acaaaacata gctaacatat ccaaaatcac tcacattagg agcttaaaaa tgacagcgat
      241 tactatccct caatttgaag aggtgacttc taagcaattt caatctgagt tcggcagaat
      301 tgttgatatc gtaaaaggca aaacccctgt ggcgattacc caatatggca gaccgacaat
      361 ggtgcttttt ccttacgatc aagcggttga agctcggcga ttattagcaa aacagcgttt
      421 gcttgaaatg cttgcagagc gagataagaa tccgccggca gctcttgcag aaatcacgct
      481 tgaagagttg gataaaatga ttgatgagga acgtaatgca ctctgattat ttagtgattg
      541 atacgaatat tttgattagt ggtttattgt tcccaaaatc aaatcccaat ttagcagtga
      601 aaaaagcctt attaggctat accatttgtg tctcgcaagc gacttttgac gaatttttag
      661 aagtctccgc tagaccgaaa tttgctaaat atttcagtat tcaggaaagg gaagaattta
      721 ttgccaattt gttggatgca gtaaagtttg tggaaattaa agaaacgatt acagattgcc
      781 aagatcccaa agataataaa tttttagaag tggcgatcag tgcaaaagcg aagtatttag
      841 tgacgggcga taaaaaagat ctcctcagta tgaaccctta tcggaatgtt gaaattattt
      901 ccgtcagcga atttttagcg gaaactcgct aatgaaacgg tttctcactc tttttcttct
      961 ccttttttca tctttttcat tagccgaaac ccaccaaatc ggcattctcg ctcagcgtgg
     1021 ggaagcctac acccgcaccc attggcagcc ttgggtggat tggttaaatt cgcagtttcc
     1081 caacgagcgg tttgagctgg ttccattggg gattaacgag gcgagccgcc gggcggagtt
     1141 ggattttctg ctgaccaatc aagctcagtt tttctatttg agtaaccaaa atgtgcgatg
     1201 gcttgccacg ctcaacagtc cgttttcggc aaatggtgag caaggggcgg tgggcagtag
     1261 catttgggtg cgtgccgaca gccattatca gcagttaagc gatctgaaaa atcagactat
     1321 cagtgcggtg gataacgatg ctttcggcgg ttttttgctt gggttgtatc agtttcatca
     1381 agcaggaatg cggcaaaatc gggatttttc ggtgcagttt agcggttttc cggtggaaaa
     1441 tgcgttggct ttgctggcgg aaaaacaggt ggaagcggcg attgtaccgg tctgtttgct
     1501 ggaagagttg gagaaagagg gcaagttcaa gcggtcggat tttcgcttaa ttttgcaaaa
     1561 cccacaggcg cagggttgcc ttgccagcac gccgttgttg ccgaactggt cgctggcggc
     1621 aatggaaaat gtgccaaacg agctggcggt acagcttgcc acacgcttgc taaacagcca
     1681 caatcccgac ctaccccgct ggacgttgcc gttttcttcc gcccaagccg atcacattct
     1741 gcgtgaactg tatcgccacc cgcaacaaaa aagcctgtgg gcaacggtgc tggattgggt
     1801 tcgtctcaat aaattcggct tgttggcggt ggtgttgttc attttgctca accttgtcgc
     1861 cctgcgttat caggtctatc gcaaaagcaa ggcgttacag caggcacact gcaaaatgca
     1921 gcagtatcag caggaactga cccgagccga ccgccttgct ttgttgggcg agatgacaac
     1981 cggttttgct cacgaactga aacagccgtt gtcggcggtg cgaatgtacg ccgaggggct
     2041 gaaaagccaa aacagcgatc cttatcaaca gcggattttg gataagctga ttgcgcaggt
     2101 agatcgggca gtgaaaacga tgcagagcat tcgggactgg gcgcaaaacc gcccgagtgg
     2161 tgaaccgcaa gcggtcattt tgaaccaatt gattactaat gtgatcgagt ttgtggcggt
     2221 ggaaaatcgc cagaatgcac aaatctcgct gattgccgac cgcacttttc ggcttaattt
     2281 acacgccacg gtactggaac aagtactgac gaactgcctg ctcaacgccc tgcaagccgg
     2341 tgcaagcgag atatcggtgc ggttacaagc ggtcgaaaac gggctggaaa ttgcaatcga
     2401 agacaacggc ggcggttttt cccctgccca gcttgaattt ccgtttgtgc catttcgcac
     2461 cgacaaacca cacggcttag ggctaggctt ggtgctatgc caacggctga tgcaatcgct
     2521 caacggacgg attgtattgg cgaacgggga gaacggggcg aaggtagtgc ttttactccc
     2581 tcaaatataa atgtaggggc aggtcctgtg cctgcccgta aatcctatca aattcagggc
     2641 gggcacagga cccgccccta cggaaaaaag aaaaaatgat tattcacatt cttgatgacg
     2701 aagaaagcat tttagacgct atgtcgttcc tgcttgcccc gcttggggtg gaaatccaaa
     2761 cgtggcaatc cagcctagat tttttggcac aggcggattt acaccggcaa ggcgtgctgt
     2821 tactagatat tcgaatgccg ctgccagacg ggcaacaggt tcatcaacaa ctgcgagagg
     2881 cacaatcgac cctggcggtg gtgattatga ccgcccacgg tgatgtgccg atggcagtcg
     2941 ctgaattgaa aaaaggcgca gtggattttc tgcaaaagcc ggcaagtttt gaacagctaa
     3001 aacaggcgat tgcacaggcg gaaaaagtaa gtgaaaaagc ggtgaaaatt cgtgaaattt
     3061 cgcaaaatta cgccaaattg acggaaaaag agcgtaacct tgtgccactg attatgcaag
     3121 gtttcaccaa taaacagatc gcagataaat tagcgatttc agtgcgtacg gtggaagtac
     3181 atcgagccaa tgtgatggaa aaaatgcagg cggagagttt ggcggggctg gtgcagatga
     3241 tcggggagtt acaatataaa ggtagcggaa attgctctgc tacctttagt ttcgactttt
     3301 gccgtattaa tttacttctt taaactgaat cattcccgct tcggcaaaat tgttatcgtc
     3361 atcttcgtat aagaaggttt tggcttcaat tttgacatca tcaaaagcgg tgtcgccttc
     3421 tatgccttca atggtttgac cgtgtttaat ccctttaaaa tcaaacaatt tcggatcgca
     3481 taagtgggat ggcacgatat cttgcaaagc actgaacatt gtttctaaac gccccgggta
     3541 ttggcgatcc caagtattca gcatttcttt caccacttgg cgttgtaaat taggctgaga
     3601 accacacaga ttacacggaa taatcgggaa ctgcttggct tcggcatatt tgataatgtc
     3661 tttttcttta caataggcga gtgggcggat cacaatttgc ttgccgtcat cagagatcag
     3721 tttaggtggc atacttttca ttttgccgcc gtagaacata tttaagaata aagtcgccag
     3781 catatcgtca cggtgatgcc ctaatgcaat tttggttgca cccaattcgg tggcggtgcg
     3841 gtataaaatc ccacggcgaa gtcgtgagca aagtgaacag gtggttttgc cctccggaat
     3901 tttctctttt acgatgccgt aggtgttttc ttccacaatt ttgtattcca cgccaatgct
     3961 ttctaaatag cgaggcaata cctcttccgg aaatcccggt tgcttttggt ctaaattgac
     4021 cgctaccaca ctaaaattca ccggtgcact ttgctgtaaa ttgagcaaaa tatctaacag
     4081 cgtatagctg tctttaccgc cggaaaggca gaccattaca cggtcgccct cttcaatcat
     4141 accaaaatcg gcaatggcat taccgacatt acggcgtaaa cgtttttgta acttgttgaa
     4201 attataagta atttttttat cagatttttg aatatcgtct gtcataatag ttgtactagg
     4261 cttagaaatt agcggtgtat agtaaagatt tcgcactatt ttgcaagtta attattcggc
     4321 ttcagcgtag cgatatagtt acaactcaca tcattattga gccaaaattt ttcggttaat
     4381 gggttgaaat ggtagcctct catctcttgg caacgtaaat cagcctgatc gcaccaagct
     4441 aacaattcgg caggcttgat gaatttttca aattcgtgcg tgccttttgg caacattttc
     4501 agcacatatt ccgccccgat aattaccaac atataggctt taaaagtacg gttgatggta
     4561 gagaaaaaca gcacgccgtc aggtttgagc agggctttac agctttgaat gatagaaagc
     4621 ggatcgggaa cgtgttccag catttccata caagtaatca catcgaattt ttcttgatta
     4681 caagcggtca tttttgctaa aaaattttca atcgtggttt gttggtaatc aatctctaac
     4741 ccactttctt tggcgtgctg ctttgccact tcaagcggtt gagaggtcat atcgatgccg
     4801 gtaacaatcg ctccctgttt tgccattgcc tcacttaaaa taccgccacc acagccgaca
     4861 tctaacacct ttttgccaaa taaaccgttc gatttttcta aaatataggc taaacgcagt
     4921 ggattaagca gatgaatcgg cttgaaactg ccgtttggat cccaccaggt tttagccata
     4981 ttttcaaatt ttgtgatttc ttgctgatcg acgttttgca taaactgttc ctatggtaaa
     5041 tttagggcat tactataacg catttagcct aaaaggaaaa tcagaatgac ccgaattaac
     5101 cttgttccgc caacggaatt atgcgatcaa catctgcttg ccgaacatcg tgaacttact
     5161 cggattccga atgcggtagc gaaaggcaaa tttagcctaa aaggacagcc agaagattac
     5221 aaactgggcg agggacacgt tcggtttttc ttcaataaac tcacgttttt gaaaaagcgt
     5281 tacgatgcgt tacacgagga atgtttagca agaggtttta aggtgcaata tttctgggcg
     5341 gaacatttac cacaagatcc ctctctctgg ctagattatc agccgactga aaccgctctg
     5401 actgtaaatc gggagcgaat tgcattaaga atgccggcaa atccacgttt tagcgaaaga
     5461 aaatgaccgc ttgtacgcct ttttgttgct ttttatgcta taatctcgcc aattttttcg
     5521 ccttatttaa ggctctttta ctaataaaat taggaaattt caatgagcga attagccaaa
     5581 gatattattc ccgtaagtat tgaggacgaa ttaaaaacct cttaccttga ctatgcgatg
     5641 tcggtgattg ttgggcgtgc cttgcctgat gtacgagacg gtttaaagcc ggtacaccgc
     5701 cgagtgcttt tttcaatgga tcaaaacagc aacacctata ataagccgca cgttaaatct
     5761 gcccgtgtgg tcggtgatgt gatcggtaaa tatcacccac acggtgactc tgcggtgtat
     5821 gacactattg tgcgtatggc tcaaccgttc tcactacgtt atatgttggt ggacgggcaa
     5881 ggtaactttg gttctattga tggcgatgca ccggcagcaa tgcgttatac cgaagtgcgt
     5941 atgcagaaaa ttacgcagga attattaacc gatctcgata aagaaacggt ggatttctca
     6001 ccgaactatg acggcaaaga gatgattcct gatgtattgc caaccaaaat tccggctctg
     6061 ttagtcaatg gttcttcagg gattgcggtc ggtatggcaa ccaatattcc acctcataat
     6121 ttaggcgaag tattagatgg ttgcttggcg tatattgaaa atgaacagat tagtgttgaa
     6181 gagctaatgc gttatatccc ggggccggat ttcccgaccg gggcgattat caacggccgt
     6241 aaagggattg aagaggctta ccgcaccggt cgtggcaagg tgtatgtgcg cgcgaaagcc
     6301 agcgtggaaa caactgataa agggcgtgag caaattgttg taaccgaact gccataccaa
     6361 gtgaataagg caaaattaat tgaaaaaatt gccgagctgg ttaaagagaa gaaaatcgaa
     6421 ggcatcagca aaattgatga ctattccgat aaaaaaggga tttcgattgt tattgaagtc
     6481 aagcgtgatg cagtgggtga agtggtgtta aaccaccttt attctttaac ccaaatgcag
     6541 gtcacgtttg gtatcaatat ggtggcgtta gataatgggc agccaaaagt gctgaactta
     6601 cgccagatca tcgaagcctt tgttaaacac cgtcgtgaag tggtcacacg ccgtacgatt
     6661 tttgaattgc gtaaagcccg tgaacgtgcc catattttag aaggtttggc gattgcgtta
     6721 gcgaatatcg acccggtaat tgaattaatt cgagcctcaa aaaccgcaga agaagcccgt
     6781 gaaggcttat tggctcgccc ttgggcatta ggcaatgttg caccaatgtt agaggctgct
     6841 ggtgttggtg cttcccgacc ggaagattta cctgaacact taggagtgcg tgatggcgaa
     6901 tactatttat ctgaagctca agccagagcg attttagagc tacgcttaca ccgcttaacc
     6961 ggtttagaac acgagaaaat cgttgaagaa tacaaagaac tcttaactga aattggcgaa
     7021 ttaatccaca ttttaaccag cccagaacgc ttaatggaag tgatccgtga agagttagaa
     7081 cgtgtaaaaa ccgattttaa tgatgaacgc cgtactgaaa ttaccgcagc ctctggcgac
     7141 atcaacattg aagacttaat cgcacaagaa gatgtggtgg ttacgctttc tcacgccggc
     7201 tatgtgaaat atcagccgtt atccgactac gaagcacaac gccgtggcgg taaaggtaag
     7261 tcggcaacca agatgaaaga agatgatttc atcgaaaaac tcttggtggc aaacacccac
     7321 gacaccattc tttgcttctc aagccgtggg cgtttatatc aattaaaagt gtatcaatta
     7381 ccgcaagcaa gccgtggggc aagaggcacg ccgattgtga atattctgcc gttagtgaaa
     7441 gaagaaaacg aacgtattac cgcgattcta ccgattccaa acggtgaatt tagtgccgat
     7501 aagtttatct ttatggcaac agctagcggt gtagtgaaaa aagtttccct tgatgccttt
     7561 agcaacgtgc gtgcaagcgg tctgattgcg ttaaaacttc gtgaaggcga tgagctgatc
     7621 ggggtagata ttactgatgg taacagcgaa attatgttgt tctcggcaca agggcgagta
     7681 gttcgctttg ccgaaacagc agttcgtgcg atgggacgta ccgccaccgg cgtacgaggt
     7741 attaaacttt ctaccaccga aatttcaagc gaagaaattg aagaagctga aattattgaa
     7801 actgacaatg aagaggcaga agattcctcc agcgtcagcc ttgataaaga ccgtgtggtt
     7861 tctttagtga ttcctcgcac cgaaggcgat attttaaccg tcacccaaaa cggctacggt
     7921 aaacgtacgg tgattagcga atatccggtg aaatcccgag caaccaaagg ggtcgtttcg
     7981 attaaagtga atgaacgaaa cggcaaagtg gttgcggcag ttcaagtaga ggaaaccgac
     8041 caaattatgt tgattaccga tgccggcacg ctggttcgca cccgagtggc agaagtcagc
     8101 ctcgttggac gaaacaccca aggcgtacgt attattcgca ccgcagaaga cgaacaagtt
     8161 gttagccttg aacgagtctg cgagcctgaa gacgatgaaa atgaaagcct agacgcatta
     8221 gaaaatgccg aaacagcaga aacatcaact gaagaataat cttaactaaa caagcggtcg
     8281 aattttcaca attttttgca aaaatagaag gaaattcgac cgtttttgga tcatacagta
     8341 gataataaaa actatggcac ttatcaatct ttccaacgcc tatttaggct ttggcgatca
     8401 tcctttattg gatcatactg aattacatat cgaacccggc gagcgggttt gtttggtggg
     8461 gcgtaacggg gcgggtaaat cgaccctgat gaaaattctg gcgggcgaag ttcagcttga
     8521 tgacggcaag ttggtatttg aaaaagacat tgtcgttaca cgtcttgaac aagatccgcc
     8581 ccgccatatt gaagaaacgg tgttcgatta tgtggcggag gggattgccc atcttgccga
     8641 cttattgaag caatatcacc acatttccct gcaaatgcag accgattaca gcgatgaact
     8701 gctcgccaaa ctttcagcgg tgcaatcgca attagaacac gctaacggtt ggcagtttga
     8761 aaaccgcatt caagacacct tgaaactgct ggaactcgcc cccgacaaac gcctgtgcga
     8821 actctcaggc ggttgggtac gccgtgcggc attggctcgg gcgttggttg ccgatcctga
     8881 tgtattgctg ttggacgaac cgaccaacca cctcgacatt gaggcgattg cgtggctgga
     8941 aaatctgtta ttggatttca aaggcagtat tatttttatc tcgcacgacc gtgcctttat
     9001 ccgcaaaatg gcgactcgga ttgtggattt agatcgaggc aaattggttt cctacccaag
     9061 caattacgat aaatacttgg aagaaaaagc cgaggatctg cgagtagaag cgttgcaaaa
     9121 tgagctgttc gacaaaaaac tcgcccaaga agaagtctgg atccgtcaag ggattaaggc
     9181 aagacgcacc cgaaacgaag ggcgtgtgcg agcgttgaaa gcgttgcgtg aagagcgtag
     9241 aaaccgccga gaggtacaag gcacggcgaa aattcagatc gacaacaccg cacgcagcgg
     9301 caaaattgtg tttgaagtgg aaaatgccgg ctatgaaatc ggcggcagaa ccctgctgaa
     9361 aaatttcagc accaccattt tgcgtggcga taaaattgcc ctgattggag cgaacgggat
     9421 cggcaaaacc acgctgatca agctgttact tggcgaaatt cagccgacat ctggttcagt
     9481 gcgttgtggc acaaaattgg aagtggcgta cttcgaccaa taccgagtcg atcttgaccc
     9541 tgaaaaaacc gtgatggaca acgtggcaga cggcaagcaa gatgtagaag taaacggggt
     9601 gaaacgccac gttcttggct atctgcaaga cttcctgttc ccgccaaaac gggcgatgac
     9661 accggtgaaa gccctctcgg gtggtgaacg caactgcttg ttgctggcaa aactgttact
     9721 caaaccgaac aacttgctga ttttggacga accgaccaac gatttggacg tggaaaccct
     9781 cgaactgctg gaagagatgc tcaccgacta tcaaggcacc ctgattattg tgagccacga
     9841 ccgtcaattt gtggataata ccgtaaccga atgctacttc ttcgagggcg aaggcgtggt
     9901 gaataaatat gtcggcggct atttcgatgc caaacaacag caggaaaatt acttcgccgc
     9961 cactgccgac aagcggttaa attcgagtaa aaatttgcaa aaaatagagg aaaaagcacc
    10021 gcttgcggag caagagaaac agaaagccga tcaggaaaaa acgaaagcag agccgacgaa
    10081 gaaaaccaaa ctttcctaca aagaacagcg tgaactggac gagttaccgg ctaaaatgga
    10141 acagcttgaa gccgacattg aagccctgca agccgaagta aatagtgccg aattcttcgc
    10201 caaagatcca agctacacca acgcccagct acaaaaactt gccgataccg aagccgcgtt
    10261 agagcaagcc tttgagcggt gggaagcgtt ggaaaatatt aaaaacggga atatctaatg
    10321 taggggcgaa tgattattcg cccttgatgg ataaaatgat tgggcgaaaa atttttcgcc
    10381 cctacaaatg tgaagcaaaa tgacagaaag attagataac tacgaacaac gttttggtgg
    10441 cattggtcgc ctttatacgc cggaaggatt ggagaaacta cgccgttcgc atatttgcgt
    10501 gattgggatt ggcggggttg gctcgtgggc agtggaggca ttggctcgct caggtatcgg
    10561 taagatcacg atgatcgata tggacgatat ttgcgtgacc aatatcaacc gccaaattca
    10621 cgcaatgagt ggcacggtgg ctcaactgaa aaccgaagcg atgaaagagc ggattgagcg
    10681 gatcaaccct gaatgtgtgg tggaaattat tgatgatttc ttaacgcagg ataatctcgc
    10741 agactacctc aatcgaggat atgactatgt gatcgatgcg attgacagtt tgcgggtaaa
    10801 agcggcgatg attgcgtttt gtaaacgcaa taagatcaaa attatcacca ccggcggggc
    10861 gggcgggcag accgatccga ccaaaattca aattaccgat ttaagcaaaa cggtgcaaga
    10921 tccgttggcg gcaagggttc gtagcctgtt gcgtaaagag tacggtttca gcaataaaaa
    10981 caaattcggc gtggagtgcg tttcttcgac tcagcaactg attttcccac aaatgggtga
    11041 ggcctgtgag gtgtcggcaa caatgaactg tgccaacggc tttggagcgg tgacaatggt
    11101 gacggcaacg ttcggctttt ttgcggtgag tcgggtgatt gataaactat taaaataaca
    11161 agcggtcata ttaaatgaat tttttgcaaa tcaaggagta aataatggca atacatcgag
    11221 tcaacccgaa agggagtatg gaacagcttt cccatttgga aatggagttg ttggcaaaga
    11281 atacgcaggg taatttgcat caactttatc gtaattgctc tttggcggtg ctaaattccg
    11341 gtgtgcatac cgatgacagc cgagcgttgt taagccaata ccctgatttt gaaatccgct
    11401 tattaacccg tgaaaaaggc gtgtcgcttg aattgcataa tccacccgaa actgcgtttg
    11461 ttgacggcaa gatgattttg aatattcaat atcacctttt tgcggtgctg cgggatattg
    11521 tgtttgtgaa tgcgttaaaa aatgcgatta gaccgcttga agagacttct ttagaagcct
    11581 taaccacgaa taccgtattt tcgattttgc ggaatgcgaa agcgattgaa atgaataccg
    11641 acccaaattt ggtggtgtgc tggggagggc attcgattaa tgaaaccgaa taccaatatt
    11701 gccgtgccgt cgggcaagag tttggcttgc gtgagctgaa tattgtgacc ggttgcggag
    11761 ccggtgtgat ggaagccccg atgaaaggag cggctatcgg acacgccaat caacgttata
    11821 aaaacgggcg ttttatcggc attaccgagc cgtctattat tgcctccgag ccgccaaacc
    11881 cgattgtgaa tgaattggta attatgccgg atattgaaaa acggttggaa gcctttgtgc
    11941 gtttagggca cgccattgtg gttttcccgg gcgggccggg aacgctggaa gagttgctgt
    12001 atattatcgg tattaagtta aacccggcta accatgctca gcaattacca ctgattttaa
    12061 ccgccccgaa agaatcggca gactatttct atgccatcga tcaatttatt ggcgaaactt
    12121 taggcgaaga agctcgcagt ttgtatgaaa ttattattga tgactcggtg ttggtggctc
    12181 gtaaagtcaa gcaagcgatg attgaggtaa aaagcagccg ttatgaaagt ggcgatgctt
    12241 ataatttcaa ctggtcgtta aaaattgagg aagattttat tacgccattt attccaaccc
    12301 acgaaaatat ggcaaacctg aatttaagtt tagcacagcc aaaagagcag ctggctgcga
    12361 atttaagacg ggcattttca gggattgttg caggtaatat taagccggag acctccgatt
    12421 taattgagaa atatggggtg tttaaattac acggcgatcg taaattaatg gagcatatag
    12481 ataaactgtt ggagcgtttt gtggttcaac accgtatgaa aattccaacg ggtgaggcgt
    12541 accaaccttg ctatgaaatt gtaaaagggt aatgctaaca aggggtaagg tatgtattta
    12601 aagcatattg aaatcagtgg ctttcgtggt attaaccatt tatcgctcca gctacgacca
    12661 aatatggtat tgattggcga aaatgcgtgg gggaaatcca gtctgattga tgcattaagc
    12721 ctgattttta accttgagca ggcgttgtat caatttcagt tcaaagattt ccatattcaa
    12781 tacaccgata cccccaacca agtgcgggac attcaactgg tgtttacctt ttgtgaagag
    12841 catcagcacg atttagcggt gcaatttccc gcttatttgg cacaagccgc gttttatatt
    12901 gataatcacc gctgtgtttc gcttgccgta accggaaaat tacacggaga gcagattagc
    12961 accgactatc aatttgtgga tcaaagccta aaaccgatcc agatagaaca agcggaaaac
    13021 gccattcggc agtttattta ccatcaccct gtttatcgcc tgcgtgatgc aaggctcaat
    13081 aacagggtgc ataatgaatt attacttaaa ggatcgtctc aacttgaaga cgattttcaa
    13141 cgtgaattaa acgccctctc ttcgctactg aaatactatt ttctaaccag ccaaagtcgc
    13201 tatttgatca aaaccagtat gcaagacacc gcattgtggt gggaaaaagt aaaatccctc
    13261 tgtttacgcc taaaacagca gccggaattg gtcgaatctg ctcagcagca tatttctact
    13321 ctatttttgt ttaatcctag atctacagta atagaaaagc cgattatttt atttgaagat
    13381 ctgaatgctc aactacaccc tcgaatgatt gcgatttttt gggaactgct cggtttttta
    13441 cccagccaac ggatcaccac tactaattct attgaattgt tatcgcaagt gcctctgcgt
    13501 gaaatctgcc gtttggtgcg tcatcacgat catacccaag cctattggct tgaacgccac
    13561 agtttaggca aggaagattt gcgaaaatta acctttcata ttcaccataa tcgtggcttg
    13621 gcactgtttg ctaaagcgtg gattttggtt gagggggaaa cggaggtttg gattttgcaa
    13681 gagctggcga gattattaga tattcatttg gaaatggaag ggatcaaaat cgttgaattt
    13741 gctcagtgtg gcttacgtcc gttaattaaa tacgccagag caatggggat cgaatggtat
    13801 gtgctgactg acggtgacga ttccggcaaa cgttatgcag atacggtaaa aagtatggaa
    13861 gaggattttg ctcaccggct cacgatgttg ccacaaaaag atatcgaaca ttttttctac
    13921 aaagcaggct tgcggaatgt gtttatacag cttgcaaatt ggcgttcaca agaaaaatat
    13981 ccggtgtcct acatcattaa acgagcgatt caacgaagct caaagcctga tttagccatt
    14041 gcattagcca atgagatggg gcaacgggga gagcattcaa ttccaacttt atttaaacaa
    14101 ttatttgcag atgtgctggc attgataaac aaagtataga atacaagcgg ttaaatttga
    14161 caaaaaattg ccaattttga ccgcttgttt gcttgtgatg aaaaccaatc atacaaaact
    14221 ctaatttatt tcaagtgcaa gctattattt tatgcgttat gcttatttgg aataaaaaaa
    14281 tcgggttagt tgattgacat agcattctag acgtctaaaa taaccaaaaa taatagttaa
    14341 ttgaatgtgg ataaatagaa aatgattcag ctgaaaaata tcagcaaaca atttgaggta
    14401 aagggcaaaa aaattaccgc tctggataat gtaaacctag aagtaccaag aggcactatt
    14461 tatggtgtca ttggggcatc aggggcagga aaaagtacct taattcgctg tgtaaattta
    14521 ttagaacgcc caacaatcgg gcaagtgatt gtcgatgggc aagatttaac ggcaatgtcg
    14581 gataaagagt tgattcacgc tcgccgtaat attgcaatga ttttccaaca ttttaatttg
    14641 ctttcatcac aaactgtatt tgaaaacgtg gcgttatcgt tgcgtttaag caatacgcca
    14701 aaagagcaag ttgagaagaa agtcaatgag ctgttggatt tagtggggtt aacagatcgt
    14761 aaagatgttt accctgcaaa tctttccggt gggcagaaac agcgtgtagc gattgctcgt
    14821 gcgttagcca gcgatccaca cgttttgttg tgtgatgaag cgaccagtgc gttagatccg
    14881 gcgactacgc aatccatttt gcagctatta aaagatatta ataaacgtct tggtttgacg
    14941 attttattaa tcactcacga aatggaagtg gtaaaacgca tttgtgaccg agtagcagtg
    15001 attgataaag ggcagttgat tgaatctggc tcggtaagtg aaattttctc gaatccgaaa
    15061 accgaattag ctcagaaatt tattcaatcg actttccatt ttgaattacc gcaagaatat
    15121 accgagcagc tttcaagttc gccgactgaa catagtaagc cgattattaa atttgagttt
    15181 accggacgtt cggtggatgc acctttgctt tcaatggcat cgaagaaatt cggcattgat
    15241 tttagtattt tgatgtcgca aattgattat gcaggcgggg tcaaatttgg atttgtaatt
    15301 gcagaagtga gtggtaatcc ggattccatt gcagaggcaa aattttattt aattgataac
    15361 aatgtaaaag tagaggtgtt aggctatgtg ggctgatttt atgcaggaga tgacgccaca
    15421 gatgtggagc ttagtatggg aatctacttt tgaaaccgtt tatatgggct ttgtggcaac
    15481 ggcattggcg gtagtcatcg gtttaccgat tggtttcttg gcgtttttaa ccggtaaagg
    15541 cgaaatttta gaaaatccga gattacacca agtgttagat gtcgttatta acatcgggcg
    15601 ttcgattccg ttcattattt tattgattat tttgttgcca tttactcgct tagttgtggg
    15661 cacaacatta ggcacaacgg cagcgattgt tcctttaagt gtttcggcca ttccgttttt
    15721 tgcccgttta acttcaaatg cgttgcttga aattccgtcc ggtttaacag aagcggcaaa
    15781 atcaatggga gcaacaaatt ggcaggtagt gacaaaattt tatttaccgg agtcattacc
    15841 gattttgatt aatggcatta cgctaacgct ggttgcttta attggctact cagcaatggc
    15901 gggggctgtt ggtggcggcg gtttaggcaa tttagcgatt agctatggcg aacaccgtaa
    15961 tatggtgtat gtgaaatgga ttgctaccat tattattgtt gcgattgtga tgattagcca
    16021 aaaattgggt gatcatttag caaaacgtta cgatcatcgt taattttatt taaaaagagg
    16081 tttctatgag tttcaagaaa attttaggcg ttgcattggt ttctgcatta gcattaaccg
    16141 cttgtaaaga agagaaaaag gcagaatcta ccgctgctcc ggcagctcaa gctccggcta
    16201 aaatcaaagt aggggtaatg tcaggccctg agcataccgt tgcagaacgt gcagcacaga
    16261 ttgcaaaaga aaaatatggt ttagaagttg agttcgtttt atttaacgac tacgctttac
    16321 cgaatactgc agtaagtaaa ggtgatttag atgcaaacgc attccaacac aaaccatatt
    16381 tagataaaga cagccaatca aaaggtttaa acaacttagt gattgtgggt aacacctttg
    16441 tttatccgtt agcgggctac tctaaaaaag tgaaaaatgt gtctgagtta gcagaaggtg
    16501 cggtaattgc agtaccaaat gatccttcaa acttagctcg tgctttgatt ttattagaaa
    16561 aacaggggtt aattaaatta aaagataata ccaatttatt ctcaacttca gtggatatta
    16621 tcgaaaatcc gaaaaactta aaaattaaag aagtggatac ttcaattgcc gcaaaagcgt
    16681 tagatgacgt agatttagcg gtggtaaata acacttatgc cgggcaggta ggcttaaata
    16741 cccaagatca cggtgtattt gttgagtcta aagattcacc gtatgtgaat attattgtgg
    16801 ctcgccaaga caataaagat gcagctaatg tacaaaactt tattaaatct taccaaaccg
    16861 aagaagtgta ccaagaagca caaaaacact ttaaagatgg tgtagtaaaa ggttggtaat
    16921 ttagctgatt tttacttaaa tggcgattaa gcgctaggtt aatcgccatt ttcatcttta
    16981 aaatatagga aggagcaaac aatgaacttt aaaaaattat taggtgtggc gttagtatct
    17041 gccttagcac ttactgcgtg taaagatgaa aaagcacaag cacctgctac aacagctaaa
    17101 actgaaaaca aagccccatt aaaagtgggt gtgatgaccg gccctgaagc ccaaatgacc
    17161 gaagtggcag tgaaaattgc aaaagagaaa tatggcttag atgtagagtt agtgcagttt
    17221 actgaataca ctcaaccaaa tgccgcactt cattctaaag atttagatgc taacgcgttc
    17281 caaacggtgc cttatttaga gcaagaagtg aaagatcgtg gttataaatt agcgattatc
    17341 ggcaatacgc tagtatggcc aatcgcggct tattctaaaa aaattaaaaa catttccgag
    17401 ttaaaagacg gagcgactgt tgcgattcca aacaatgcaa gtaatactgc tcgtgcgtta
    17461 ttattgcttc aagctcacgg tttattgaaa ttaaaagatc cgaaaaatgt gtttgctacc
    17521 gaaaacgata ttatcgaaaa cccgaaaaat atcaaaatcg tacaggcgga tacctcactt
    17581 ttaacccgta tgttagatga tgtagaactt gcggtaatca acaacactta cgcaggtcaa
    17641 gctgggttaa gtccggataa agacggtatt attgtggaat ctaaagattc accgtatgtg
    17701 aatttagtgg taagtcgtga agataataaa gatgacccac gcttacaaac ttttgtgaag
    17761 tcattccaaa ccgaagaagt attccaagaa gcgttaaaat tatttaacgg tggtgtggtg
    17821 aaaggttggt aatcagttgg acggcatatt ctgccgtcct tttcttaaaa ggagaaacag
    17881 ataatgaaaa taatgaaatt agccggtgca gttgcaattt tctcgctttt tttaaccgct
    17941 tgtaatgata aagccgaaaa gttgaaagtc ggtgtgattt ccggccctga acataaggta
    18001 atggaagtgg cggcaaaaat tgcaaaggaa aaatataacc gtgatgttga attagtggta
    18061 tttaccgatt atgccacgcc taatgcagct ttagataaag gcgatcttga tttgaatgct
    18121 ttccagcata aaccttattt agataaccaa attcaggaaa aaggctataa attagtgccg
    18181 gtcggcaata cttttgttta tccgattgcg gcttattcca aaaaaattaa atcgctggca
    18241 gagttgaaag atggtgatac tattgcagtg cctaatgatc cgactaattt agcccgtgct
    18301 ttaattttat tggaaaaaca agatttaatt aagctgcgag cagatgcagg cttaaaagca
    18361 accagtgtgg atattattga aaaccctcgt aaattggtga tccaagaaat tgaagcacca
    18421 ttattgcctc gaacgttgga cgatgttgcc ttttcgatta ttaatactac ctacgcaggg
    18481 caaaacggtt taacgccaac caaagacgga atattcgttg aagacaagga ttcgccttat
    18541 gtgaatttaa ttgttgcccg tgaaaataac caacattctg aagccgtaaa agatttggtg
    18601 aaagcctacc aaacagaaga ggtgtataac aaagcgaatg aagagtttaa aggggcgatg
    18661 ataaaaggct ggtaattgaa gataaataag gcatagattc ctatgcctta ctttttatgt
    18721 tacagacctg ctccggctct taatgcttcg gctttgtcag ttttttccca aggaaaatgt
    18781 ttacgaccga agtgtccgta ggcagcggtt tcacggtaga ttggtttgat taaatctaac
    18841 attttgatta agccatacgg gcgtaagtca aagttttggt agattaattt cacaagtatt
    18901 tcgttgctca ctttacctgt gccgaaggtt tcgaccataa ttgatgttgg gtcagccacc
    18961 ccgatagcat aagaaagttg gatctcacag cgatcggcta agcctgcggc aacgatattt
    19021 tttgcgacat aacgagcagc ataagcagca gagcgatcca cttttgacgg gtctttacca
    19081 gagaatgcac caccaccgtg gcgagccgca ccaccgtagg tatctacaat gattttacgc
    19141 ccggttaaac cacagtcacc cattggaccg ccaatcacaa aacgaccggt tgggttgatg
    19201 aaatatttgg tgttttggct gagccattcg cttggtagta ccggtttgat gatctcttcc
    19261 atcacccctt caattaactc tttttgcgaa atatgctctg cgtgttgtgt agaaagcact
    19321 acggcatcaa tgccgataat tttattgttc tcataagcaa atgttaattg gcttttagcg
    19381 tcagggcgta accaatcgag tttgcccgat ttacgcactt ttgactgctg ttccattaag
    19441 cggtgtgcgt aagtaattgg agccggcatt aacacctcgg tttcattggt agcgtagcca
    19501 aacataatcc cttggtcgcc cgcaccttgc tctaacggat cggcacggtc aacgccctgg
    19561 ttgatgtccg gagattgttt accaatcgca tttaataccg cacaagagtg agcatcaaag
    19621 cccatatcag agtgagtata gccgatgtcg caaatcactt ggcgggttaa gttttcaata
    19681 tccacccacg cagaggtagt gatttctccc cctactaatg ccataccggt tttcacataa
    19741 gtttcacaag caacgcgtgc tttagggtct tgttttaaaa tttcatctaa taccgcgtct
    19801 gaaatttggt ctgcgatttt atccggatgc ccttctgaaa ccgattcaga agtaaataaa
    19861 ttaattgcca tttgtattcc tctctatctt aaatatggat tattaatagt aggggcgggt
    19921 ccagtgcccg cccataaatt atataaaatg tttggacagg cacaggaccg gtccctataa
    19981 aatttgtttg gaagtgtttc cgtctagacg tctataatat attttttgaa cgttttaggc
    20041 aaatagaatt tgtttatttg gctaaatatg cccttaaacc agccaacgca tttttggcat
    20101 cttctacccc taaatcgtac attgcttgta ttttatttac atccgtttca aggcgactga
    20161 ttttgactgt tctgcttgga cgaatcacaa aaatctgttg tttttcattg agttgaataa
    20221 tctgctcaac ggcttgatta tattccgcat agcggttttc ccagcgttgc actaatttcg
    20281 gatatttttt ataaaaacgt ttaaacagcc acatcgagct tggtttctta cggtattcaa
    20341 gtgggcgagt aagcaccacg ataattttgt catatcccat ttcaataaat ttctgcaccg
    20401 gaatactgtc tgacacacca ccgtctaaat attttttacc ctcaatttcg actattttgg
    20461 atacaatcgg cattgaggaa gtagctcgaa aggcttccat ttgctcgaac acattgtcaa
    20521 ttttgatgta ttcgggctca ccggtttcga cattggttac agtaacccaa aagtccatac
    20581 cggaatcctc aaaggcttgt tggtcaaacg gatccagttt ggaagggagt tcatagtaag
    20641 caaaatcccg attaacgata ttgccggtgg taagcaagct gtgtaacccc atataccgct
    20701 tatctttagc gtattttttg ttgtatcgca acgccctacc tttttgtttt gaaggcaaat
    20761 tcacgccaaa taacgcaccg gctgaaacac cgattgctcc atctatatga atatgttcat
    20821 ctaaaaatac atctaacacg ccggcagtaa aaatacctcg caaaccaccg ccttccagca
    20881 ctaatccaac tttcatatca ttctccctgt aatttgtgta ttataacccg aattatcttg
    20941 ctaggagaaa tcacaatggc tgatgcagaa tttatggatt tcctgttaga tcaactaagc
    21001 ccgatttccg gtttgcgttc gaaaaaaatg tttggtgaat attgcttgtt ttttgataat
    21061 aaaattgtcg ctattatcag caaagattat cgcatttttg tcaaagctaa tccgcaaacc
    21121 ttgccgttat tcttagctga aaatgccgaa caatttcact attttgctaa aggaaaagtg
    21181 caaaaaatgc actactggag cattccggaa ttagcgattg aagaagccga tgaactggtc
    21241 aaatggattc ggctaggctt
//
LOCUS       DANLYI010000016        20904 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.016, whole genome shotgun sequence.
ACCESSION   DANLYI010000016 DANLYI010000000
VERSION     DANLYI010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 20904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20904
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.016"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            <1..406
                     /locus_tag="PXD81_000481"
     CDS             <1..406
                     /locus_tag="PXD81_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HDL5536545.1"
                     /translation="RGYHWNVRGVNFFELHAKFEEIYDDLVTKVDEIAERILTLGHTP
                     QNAFSQYLTKSQIQEHIGVSAAQECLRGTLNGFKTLLGQQREILALANDANDEGTASQ
                     MSDYIKEQEKLVWMFTAACAECSDYAQGCSCN"
     gene            465..1178
                     /locus_tag="PXD81_000482"
     CDS             465..1178
                     /locus_tag="PXD81_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011609450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HDL5536546.1"
                     /translation="MAMSQGFQFKQFFIRHDRCAMKVNTDGILLGAIAHIKDCQNILD
                     LGIGSGLVALMLAQRTDDCCHITALELEPNAYQQAVENAQNSAWANRISVLQGDVMQQ
                     AFEQKFDLIVSNPPYFADSLAARTTERDLARSISHYSHLNWLAKAKPWLAENGKITLI
                     LPIAAAEKLVDQSEQIGLYCVEQWLIFTKSGKLAKRSIVSFSLNKQKREHKELIIYDL
                     DNCYTTEFKRLTQDFYLNF"
     gene            complement(1289..2293)
                     /locus_tag="PXD81_000483"
     CDS             complement(1289..2293)
                     /locus_tag="PXD81_000483"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005651072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HDL5536547.1"
                     /translation="MAFNLKNRHLLSLVNHTEREIRFLLDLARDLKRAKYAGTEQQKL
                     KGKNIALIFEKTSTRTRCAFEVAAYDQGAHVTYIDPNSSQIGHKESMKDTARVLGRMY
                     DAIQYRGFKQSVVQELADYAGVPVFNGLTDEFHPTQMLADVLTMIENCEKPLSQISYV
                     YIGDARNNMGNSLLLIGAKLGMDVRICAPKALLPEDAFVKMCEKFAAESGARMTVTED
                     IETAIKGVDFVHTDVWVSMGEPLESWTERIDLLMPYRVTPELMQATDNPKVKFMHCLP
                     AFHNSETKVGKEIAEKYPALANGIEVTEEVFESPMNVAFEQAENRMHTIKAVMVASLA
                     "
     gene            complement(2381..3220)
                     /locus_tag="PXD81_000484"
     CDS             complement(2381..3220)
                     /locus_tag="PXD81_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HDL5536548.1"
                     /translation="MQSYPYLLVNVFATSHFGGNPLAVFYEADGLTDLQMQLIARQFN
                     LSEVVFIQSSTDSQAVKKLKIFTPDYEMPFAGHPTVGAAFVLHSAFDFPTTYRLQTNA
                     GLVAIQHQHNVTTFALTNGVNLENTPLSRAECASILGLNVADIASEPCYVNTGVEQLL
                     VELASQQAVEKCKINANLAEKAFLKDSVYVWCREGNEAKVRLFFQSQGSIVEDPGTGS
                     AAANLGGWHIAQGKIPIQLQISQGDEIQRPNRLLLKVDQNGKIFVGGEVILVGKGEFY
                     LPE"
     gene            3410..4744
                     /locus_tag="PXD81_000485"
     CDS             3410..4744
                     /locus_tag="PXD81_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005617788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter"
                     /protein_id="HDL5536549.1"
                     /translation="MLYAEFLLLLAFLYAGSRYGGIGLGVVSGIGLFVEVFFLGMPLS
                     APPISVMLVILAVVTCASILEAAGGLKFMLQVAERILRSNPKRITFLGPLVTYLMTLM
                     LGTGHSVYSIMPIIGDIALKNKIRPERPMAAASVASQLGITGSPLSAAVAYYLTEIVK
                     LPGFEGVTLLNVVGVTITATFCGVIAMSLYSLRRGKELEDDPEYQRRMQDPELRKQIE
                     QTSATSLDEKLPASAKNSVYLFLAAIATIVIIAMIPAIKPTVAATGKVAGMDQIIQIV
                     MLTFGGLILILTKTDPKKVPNGIVFKSGMVATIAIFGIAWMSDTYFTHAMPAFKEGVT
                     EMVRAQPWTFAFALFAVSVVVNSQAVTAKMLLPVGIAMGLPAPLLVGLMPATYAYFFI
                     PNYPSDIATVNFDVTGTTKIGKYYFNHSFMAPGLVGVITACIVGIAVANVLI"
     gene            complement(4824..5234)
                     /locus_tag="PXD81_000486"
     CDS             complement(4824..5234)
                     /locus_tag="PXD81_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HDL5536550.1"
                     /translation="MNSTLTNLQPLAFLITRVVAGYMFLLHGTAKFFEFPMSMTNGNG
                     SVPLASIYGVGGILEIVGGIFFILGLFTRQTAFILSGMMAYAFFIAHGAQGNVLLPLL
                     NGGELAGVYSVLFLTYIFIGGGKYSLDAKLFNKA"
     gene            complement(5357..6034)
                     /locus_tag="PXD81_000487"
     CDS             complement(5357..6034)
                     /locus_tag="PXD81_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HDL5536551.1"
                     /translation="MNLFGFRCFHCNHPLAIGVQGICSHCQKLLKPTPYCRHCGMSAV
                     EFRNACGYCLKNEPKWHKLVRVNEYRAPLSHWVHQFKFQQQYWWDQALARQLLLAIKQ
                     AQREQVLSLPEVIMPVPLFWHRHWKRGFNQSELLCDWLSKWLQIPLDTQSLQRVRATV
                     SQRELTAAERRKNLRGAFFYQPLKAYKRVAIVDDVLTTGSTLNVICAELLKAGVVEIQ
                     VWVLARA"
     gene            6152..6964
                     /locus_tag="PXD81_000488"
     CDS             6152..6964
                     /locus_tag="PXD81_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005610932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDL5536552.1"
                     /translation="MKSLQKPIKIVFFDIDDTLYVKHKAYIPKSITEQVLPRLKAKGI
                     IPAIATGRNHGCFPEALKPYLNPEIGFELFVTINGQYNFYKDQIISDYSLSREQIERI
                     IEKANALGILYAFITPMTIAVSETNPIVHEAVLPITPNYLIDPHYYEKEKIVQMLLFY
                     TEEQKQAVENSGIFANDLKEARWHPNAVDILRKENSKARGIQDVLATLNIEIESAMAF
                     GDGINDLEMLPTVGVGVAMGNGVPELKAVADFITKPLEEDGILYALETLEVI"
     gene            complement(7012..7281)
                     /locus_tag="PXD81_000489"
     CDS             complement(7012..7281)
                     /locus_tag="PXD81_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HDL5536553.1"
                     /translation="MGLLYLLLLNTLIGYGSYGEALNHWDTSKVSIVTTMLPIFTMIF
                     SNLSYYFYPHIFAKPDMNWISYLGALVVVSGAILAIAGDKLFRRN"
     gene            complement(7254..7931)
                     /locus_tag="PXD81_000490"
     CDS             complement(7254..7931)
                     /locus_tag="PXD81_000490"
                     /inference="COORDINATES: protein motif:HMM:NF013088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDL5536554.1"
                     /translation="MKQRPLLGFSLALTAAMTWGTLPIAAQQVLKVIDAQTLVWVRFV
                     VASVGLLLILGFAKRLPKLTACSKKELGLILLGVCGLSANFFLFAEALHYVSPTINQV
                     LWQLAPFSMILLGVLLFKEKFGLFQKIGFVLLIAGLVAFFNDKFAEILQLNTYALGVL
                     LSAGASLIWVLYGVAQKLLLAKFNSQQVLFIIYIGCSLLMLPFASPAQLGNLEDGFLW
                     GCFIYCC"
     gene            complement(8033..8290)
                     /gene="rpmA"
                     /locus_tag="PXD81_000491"
     CDS             complement(8033..8290)
                     /gene="rpmA"
                     /locus_tag="PXD81_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007369914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HDL5536555.1"
                     /translation="MATKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGSNVGMGKDHTLFATADGKVKFEVKGEKSRKYVSIIAD"
     gene            complement(8311..8622)
                     /gene="rplU"
                     /locus_tag="PXD81_000492"
     CDS             complement(8311..8622)
                     /gene="rplU"
                     /locus_tag="PXD81_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HDL5536556.1"
                     /translation="MYAVFQSGGKQHRVSEGQVVRLEKLELATGEKVEFDSVLMVVNG
                     EDVKIGAPVVAGAKVVAEVVAQGRGEKVKIVKFRRRKHSRKQQGHRQWFTEVKITGIQ
                     A"
     gene            8900..9898
                     /gene="ispB"
                     /locus_tag="PXD81_000493"
     CDS             8900..9898
                     /gene="ispB"
                     /locus_tag="PXD81_000493"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HDL5536557.1"
                     /translation="MAVQLFSLEQIQALANEDMQAVNAEILAQLNSEVLLINQLGHYI
                     IAGGGKRIRPLIAVLAARALGYRGKKHITAAAFIEFVHTATLLHDDVVDESDMRRGRP
                     TANAHFGNAASVLVGDFIYTRSFQMMTDLDSLRVLKVMSAATNALAEGEVQQLMNVND
                     PNTTEANYMRVIYNKTARLFEAATQCVAIAADASEEIETALKNYGCYLGTAFQLIDDI
                     LDYSAQSESLGKNVGDDFVEGKPTLPLLHAMHAADKAGKTTQAELIRNAIENGGELEQ
                     IPQVLAIMQEHQSLDYAMQKAREEAQKAVDSLGVLPESEYKQALVSLAYLSVDRSY"
     gene            10001..10747
                     /locus_tag="PXD81_000494"
     CDS             10001..10747
                     /locus_tag="PXD81_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HDL5536558.1"
                     /translation="MTEQNQRPQEHIESVRARRRAQNQKKGPNAPFVREKLTLPNGHN
                     KLLLHSCCAPCSGEVMEAIHASGIEFTIYFYNPNIHPLKEYLIRKEENIRFAEKWGIP
                     FIDLDDDYENDRKEWFKKAKGMEWEPERGIRCTMCFDMRFEKAAEYAHNNGFPVFTSC
                     LGISRWKDMNQINDCGHRAAAKFDDVVYWDYNWRKGGGSQRMIEISKRERFYQQEYCG
                     CVYSLRDSNKWRLETGRQRIEIGKLYYSPD"
     gene            11164..12099
                     /gene="fabD"
                     /locus_tag="PXD81_000495"
     CDS             11164..12099
                     /gene="fabD"
                     /locus_tag="PXD81_000495"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014990844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HDL5536559.1"
                     /translation="MSKFAMVFPGQGSQAVGMLADLAPQYPIVEETFKQASDVLGYDL
                     WDLVQNGTAEELGQTHRTQPALLASSVALFRIWQQKFPEQKPAVMAGHSLGEYSALVC
                     AGVLDFQDAIKLVELRGRAMQEAVPAGSGAMFAIIGLDNESIIKACEQAASETGEIVS
                     AVNFNSPGQVVIAGTKAAAEKAGELCKAAGAKRALPLAVSVPSHCALMKPAAEKLAEA
                     LQNIELNQPLVPVINNVDVAVETDADTIRNALVRQLYSPVRWTETVEKMAQDGITTLY
                     EIGPNKVLTGLAGRIVKELSAKAVNDVASFEAVEA"
     gene            12116..12580
                     /locus_tag="PXD81_000496"
     CDS             12116..12580
                     /locus_tag="PXD81_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5536560.1"
                     /translation="MSKETLFMLISALIALVILPLAYTVGIAKETLGGNMGMLSIAAG
                     CSFLAYFLSLNPKIRHAFIGGSAITAAAIQAWVLYGVLLSENDPKSAVVLFQIYLFSS
                     LGPLFFSAIWAFTRPRWIYPKQGSAVKYALFGGLTTVINYLITLFIFYILTL"
     gene            12601..13323
                     /gene="fabG"
                     /locus_tag="PXD81_000497"
     CDS             12601..13323
                     /gene="fabG"
                     /locus_tag="PXD81_000497"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HDL5536561.1"
                     /translation="MSKIALVTGATRGIGKAIAEELVAKGYIVVGTATSEKGAESISA
                     YLGENGKGLVLNVADAESIESVLKQIKETFGDVDVLVNNAGITRDGLLMRMKDEDWFD
                     IIQTNLTSVYRLSKAVLRPMMKKGGRIITIGSVVGSMGNPGQTNYCAAKAGLVGFSKS
                     LAKEVASRGITVNVVAPGFIATDMTDELTEDQKNAILSQIPAGELGSPQDIAKAVAFL
                     ASDDARYINGETIHVNGGLYMS"
     gene            13442..14740
                     /gene="brnQ"
                     /locus_tag="PXD81_000498"
     CDS             13442..14740
                     /gene="brnQ"
                     /locus_tag="PXD81_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HDL5536562.1"
                     /translation="MTRNTLVVGFLLFAIFFGAGNLIFPPKLGYESSTEFFSAISGFV
                     LTGVGLPLLGIIVSAFYNGGYKSALDKIHPWFSLAFLTSIYLAIGPFFAGPRTAATAY
                     EMAIMPFLGDSSQVSMLIFTLIYFAFTLWLALNPNKMVDRIGAILTPILLITIIGLVI
                     RAGFILDTDPQITTPNTNEQSYFFKGMVDGYLTMDALASLAYSVIVLNAIRGKGVSQA
                     ALQRQTVSAGLVAAVALAVIYISLGWIGNNMPISANTLSMLSERKLDLGTYILNTVTT
                     QAFGELGRSVLGVIVSLACLTTAVGLAVSVSEYFNEIFPKISYKVYAILFTVISFTVA
                     NQGLKEVISKSIPVLLVLYPIAMTVILMLLINLVIKLPLSAQRLSLLLVTIVAIASVA
                     GVGFTENLPLKSYSMEWLPFAIIGAIIGAIIHPLTGRKHK"
     gene            14786..15610
                     /gene="mutM"
                     /locus_tag="PXD81_000499"
     CDS             14786..15610
                     /gene="mutM"
                     /locus_tag="PXD81_000499"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005713993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HDL5536563.1"
                     /translation="MPELPEVETSVRGVSPYLVGQTIEQIIVRQPKLRWTVSPELSQM
                     AGAKITAVERRAKYLIIRTDKGDILVHLGMSGSLGILQERQQKEIGKHDHVDLITQNG
                     TILRYNDPRKFGCWLWAEKAEDHELLRKLGPEPLSNDFTADYLFAKSRKKTTACKTFI
                     MTNEVVVGVGNIYACESLFMAGIHPELAAQNLTKKQCERLVKTIKEVLTKAIIQGGTT
                     LKDFIQPDGKPGYFAQVLQVYGRKGETCNDCGKPIETKVIGQRNTYFCPKCQRLPK"
     gene            complement(15693..16127)
                     /gene="dtd"
                     /locus_tag="PXD81_000500"
     CDS             complement(15693..16127)
                     /gene="dtd"
                     /locus_tag="PXD81_000500"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005602846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HDL5536564.1"
                     /translation="MIGLIQRVKFAKVEVEGQVVGEIASGLLVLLGVEKDDDEAKADK
                     LLEKVLNYRVFSDSEGKMNLNVQQAGGSLLVVSQFTLAADTNKGLRPSFSKGASPVDA
                     ERLYAYFHQQAATKIHTQTGQFAADMQVSLQNDGPVTFWLQV"
     gene            complement(16102..16947)
                     /locus_tag="PXD81_000501"
     CDS             complement(16102..16947)
                     /locus_tag="PXD81_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HDL5536565.1"
                     /translation="MLNRLWRFIKLFAKRYNENNIAISAGYLTYSTMLAIVPFIVVLF
                     SIFTFFPIFHEANEQLKAFIYDNFAPSASEMVSEYIELFVSNAKQMGLVSFIGLFVVT
                     IMLIKSIDEALNTIWHDNRQRPMWFSFLLYFLILLFGPLLAGASIAISTYVMSLSIFN
                     GNGALSLGQHLLELMPFLLIWLLFTLVYKFIPIRKVKFQHAAIGALMAAIFFTLGKKA
                     FIWYITAFPSYQAIYGALAVLPIMLLWIQLSWQVVLLGGQFAAVLDEMETDKGSINDR
                     LNSAG"
     gene            complement(16949..18187)
                     /gene="proA"
                     /locus_tag="PXD81_000502"
     CDS             complement(16949..18187)
                     /gene="proA"
                     /locus_tag="PXD81_000502"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HDL5536566.1"
                     /translation="MQMIELAQQAKQASFELAQFGNLQKNQALLTIADSLEQRAEEIL
                     YANAKDIEFAQAQGISLAIIDRLLLNEDRLKDIANDVRNVAKLADPVGQVMDGGVLDS
                     GLKIERQRVPLGVILTIYEARPNVTIDVASLCLKTGNAVILRGGKETKFTNAVLVDVV
                     QDALEKAGLPKLAVQAVTDPDRALLLELLKLDRYIDMVIPRGGAGLHQFCKENSTIPV
                     IVGGIGVCHLFVEKTADQEKSLEVIANAKTQRPSTCNTLETLLVEKAIAVEFLPKLVA
                     KMQQLGVTIHSNDLQKIEGIEPLDQAKLSQEWLSLDLNVVVVNDLQQAVEHIRHYGSQ
                     HSEGILTSDYQLARQFVQQVDAAAVYINASTRFTDGGEFGLGAEVAVSTQKLHARGPM
                     GLEALTTYKWVCEGDYLVRG"
     gene            18471..20354
                     /gene="mutL"
                     /locus_tag="PXD81_000503"
     CDS             18471..20354
                     /gene="mutL"
                     /locus_tag="PXD81_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HDL5536567.1"
                     /translation="MINTTPKQLIHILPPQLANQIAAGEVVERPASVVKELVENSLDA
                     GANTIQIDIEKGGSQLIRIRDNGCGISKQDLPLALARHATSKISSLEDLEAILSLGFR
                     GEALASISSVSRLTLTSRTAEQTEAWQAYAQGREMAVEIQPASHPIGTTIEVANLFFN
                     TPARRKFLRTDKTEFAHIDEVVRRIALAKPNISFSLSHNGKIVRQYRKVQDNSVEQQQ
                     KRVAAICGDEFIQHSTYIDRQHGDLHLHGWVGSPALARLQNDLCYSYVNGRMMRDKTI
                     NHAIRQAYGDSIASGYYPAFVIFLDLDPTMVDVNVHPAKHEVRFHQGRLVHDFILQGV
                     LNSLQQTQPTLELRNEVNEPLPSYVQDTNRQAAGQNIFVSQTAPQAAEFTQKNASRSS
                     YTPKFERSNSVSSSAQKWYKELVGGDLHQTTPTIKTQSTLVEPKTEPQTEHKTEAVSQ
                     KITQIAPLASHHSQALAIVKNQAVLIKEEENFYLLPLATLAELKLKLQLEKGQSQALL
                     IPLNLSLDQQQTERWQQTKEELTKLGFSISEKQWQAQTRLTVLSVPQAVREQNLQQLL
                     FALFNQTQAVNLAEFFAKKCEKPTACTLSDVIALLSEIELYANGKKELEGIRKEVDFS
                     KFL"
     gene            complement(20373..20642)
                     /locus_tag="PXD81_000504"
     CDS             complement(20373..20642)
                     /locus_tag="PXD81_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin, RelE/StbE family"
                     /protein_id="HDL5536568.1"
                     /translation="MREIEYSNAFKRDYKKCAVESLSSSLIEALYFLINDKPLPEKYC
                     DHALTGEWKGFRDCHIQPDLVLIYRKVEDDKLELLRIGSHSHLFG"
     gene            complement(20629..20895)
                     /locus_tag="PXD81_000505"
     CDS             complement(20629..20895)
                     /locus_tag="PXD81_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HDL5536569.1"
                     /translation="MANSALVSVRISPEIKEQASEVLAGIGLTVSDVMRMTLAKIATE
                     KRFQFDYQPNFETAEVLRAVKNGEEKLHSANSIADLMEQLNARD"
BASE COUNT         6191 a         4588 c         4270 g         5855 t
ORIGIN      
        1 acgtggctac cattggaatg tacgtggtgt gaacttcttt gaattacatg caaaatttga
       61 agaaatctac gatgatttag tgacaaaagt agatgaaatt gcagaacgta ttttaacctt
      121 aggtcacaca cctcaaaatg cgttcagcca atatttaacc aagtcacaaa ttcaagaaca
      181 tattggtgtt tcagctgcac aagaatgttt acgtggtact ttaaatggtt tcaaaacatt
      241 actgggacaa caacgtgaaa ttcttgctct agcaaatgat gcaaacgatg aaggtactgc
      301 ttctcaaatg agtgattaca tcaaagaaca agaaaaatta gtatggatgt tcactgcagc
      361 ttgtgctgaa tgttcagact acgcacaagg ttgttcttgt aactaagttg caataatata
      421 attaaccccc gataatgaaa gattatcggg ggttactttt tcagatagct atgtcgcaag
      481 gatttcaatt taaacagttt tttattcggc acgacagatg tgcaatgaaa gtcaatacag
      541 atggtattct attgggagcg attgctcaca taaaagattg tcagaatatt ttagatctgg
      601 gtataggttc tggtttagta gcacttatgc ttgctcagag aacagatgat tgttgtcaca
      661 ttactgcgtt ggaacttgaa ccaaatgcct accaacaagc ggtcgaaaat gcccaaaatt
      721 ctgcatgggc aaatcggatt tccgttttgc aaggtgatgt gatgcagcag gcatttgaac
      781 agaaatttga cttgattgtt agtaacccac cttattttgc agattcactt gcagctcgaa
      841 ccacagagag agatttagct cgctcaatta gccattacag ccacttgaat tggctggcaa
      901 aagcaaaacc atggttagca gagaacggga aaattacact gattttacct atagcagctg
      961 cagaaaaatt agttgaccaa agtgaacaaa ttggattgta ttgtgttgaa caatggttaa
     1021 tttttaccaa gagcggcaaa ttggctaaac gcagtatagt gagttttagt ttaaacaagc
     1081 aaaagcgtga acataaagag ctgatcatct atgatctaga taattgttac actactgagt
     1141 ttaaaagact tacccaagat ttttatttga atttttaatt tactccccgg gttagtgttt
     1201 tccacaaatc ttcttcagct acgatgatct agccttaagc taaaaagcag attaccaccc
     1261 aagataatcc gctttttaga cttagctatt atgccaaact tgctaccatt accgctttaa
     1321 tagtgtgcat tcgattttct gcttgctcga atgccacatt cattggcgat tcaaacacct
     1381 cttcggttac ttcaatgccg tttgccagtg ccggatattt ttcggcgatc tctttaccga
     1441 ctttagtttc actgttgtgg aacgccggta agcagtgcat aaatttcact ttcgggttgt
     1501 cggttgcttg cattaattca ggcgttactc ggtaaggcat taataaatct atacgttcag
     1561 tccagctctc tagcggttca cccattgaaa cccaaacatc agtatgcaca aaatcgacac
     1621 ctttaattgc agtctcaata tcttcggtta ccgtcattct tgcccccgat tcagctgcaa
     1681 atttttcaca cattttcacg aatgcatctt ccggtaataa ggctttcggg gcacaaattc
     1741 gcacgtccat ccccaatttc gccccaatca acaggagtga attacccata ttattgcgag
     1801 catcgccgat atagacataa ctgatttggc taagcggttt ttcgcaattt tcgatcatcg
     1861 ttagcacgtc agccaacatt tgggttgggt ggaattcatc ggttaagccg ttaaacaccg
     1921 gcacgccggc ataatcggca agctcttgta ctaccgattg tttaaagccg cgatattgaa
     1981 tcgcatcata cattctgcct agcactcggg cggtatcttt cattgattct ttatgcccga
     2041 tttgcgagga gttcgggtca atataggtaa cgtgagcccc ttgatcgtaa gctgctactt
     2101 caaaggcaca acgagtacgg gtcgaggttt tctcaaaaat taaggcaata tttttgccct
     2161 ttaatttttg ctgctcagtg ccggcatatt tcgcccgttt taaatctctt gctaaatcca
     2221 acaaaaagcg aatttcacgc tcagtgtggt ttactaaact gagtaaatgt ctgtttttta
     2281 agttaaatgc cattttgttt ctccctgtaa acattaaaat ttattttatg caaaataatt
     2341 gcataaaaat tcacttatag caaacagaat ttgcttaccc tcattcgggt aaataaaact
     2401 cgcctttgcc gactaaaata acctctccgc caacaaaaat cttcccgttt tgatcgactt
     2461 ttaataacag gcgattcggc cgttgaattt catcaccttg tgagatctga agctggattg
     2521 gtattttgcc ttgtgcaatg tgccaaccgc ctaaatttgc cgctgccgaa ccggtgccgg
     2581 ggtcttcgac aatcgaacct tgcgattgaa aaaacagtcg cactttcgcc tcatttccct
     2641 cacgacacca aacatataca ctgtctttca aaaatgcttt ttcagccaga tttgcattta
     2701 ttttgcactt ttcgaccgct tgttgagaag ctaactcaac caataactgc tccacgccgg
     2761 tgtttacata acaaggctcg gacgcaatat ccgcaacatt cagccccaat attgaggcac
     2821 attctgcacg gcttagcggt gtgttttcca ggttcacacc attggttaaa gcaaaagtag
     2881 tcacattatg ttgatgttga atcgctacca atccggcatt agtttgcaag cgataggttg
     2941 ttggaaaatc aaaggcggaa tgcaacacaa aagccgctcc aacagttggg tggccggcga
     3001 acggcatttc ataatcaggc gtaaaaattt tgagtttttt gaccgcttgt gaatcggtgg
     3061 aagattgaat aaataccact tcagaaaggt taaattggcg agcaataagt tgcatttgta
     3121 agtcggttaa tccgtccgct tcataaaata ctgcaagcgg gttgccgcca aaatggcttg
     3181 ttgcaaacac attcaccagc aaataaggat agctctgcat ttatcactcc ttacataaac
     3241 ttaaagccac attgggcgaa aattctacca ttttggtggc gaatcgtaaa attggaaaat
     3301 ttatcggtat ccctctttag agttaactat ttttgattta tctcaaagtt ttaaaaaaaa
     3361 gattaggtca tacttctttc tagtgattat tttttaattt gaggttctta tgctttatgc
     3421 agaattttta cttttattag cctttttata tgcaggtagc cgttacggtg gcatcggttt
     3481 aggtgtggtt tccggcatcg gcttatttgt tgaagtgttc ttcttaggaa tgccgctttc
     3541 cgctccgcca attagcgtga tgctcgtgat tttggctgta gttacttgtg cctccattct
     3601 agaagccgct ggcggtttga aatttatgtt gcaagttgcc gaacgcattt tgcgtagcaa
     3661 cccaaaacgt attacctttc ttggcccact tgtcacttat ctgatgacct taatgctcgg
     3721 cacagggcat tcggtttact ctattatgcc gattatcggt gatattgcct taaaaaataa
     3781 aattcgcccg gaacgcccga tggcagcagc ctctgttgct tcacaattag gtattaccgg
     3841 tagcccactt tctgctgcag ttgcctatta cttaaccgaa atcgttaaat taccgggctt
     3901 tgaaggggtg actttattga atgtagtcgg cgtaaccatc accgcaacct tctgtggtgt
     3961 gattgcgatg tcattatata gcttacgtcg tggtaaagag ttggaagacg atcctgagta
     4021 tcaacgccgt atgcaagacc ctgagctacg caaacaaatc gagcaaacga gtgcaacgtc
     4081 tttagacgaa aaactaccgg caagtgcgaa aaactcggtt tatctgttcc tagccgctat
     4141 tgcaaccatt gtgattattg caatgattcc ggctattaaa ccaacggttg ctgctacagg
     4201 aaaagttgcc ggtatggatc aaatcattca aatcgtgatg ctaactttcg ggggtttaat
     4261 cttaatttta actaaaaccg acccgaaaaa agtaccaaac ggcatcgttt tcaaatccgg
     4321 tatggtagca accattgcga ttttcgggat tgcttggatg agtgatactt acttcaccca
     4381 tgcaatgcca gcgttcaaag aaggggtaac agaaatggtt cgagcccaac cttggacatt
     4441 cgccttcgcc ttgtttgcgg tatctgtagt agtaaacagc caagccgtaa ccgcaaaaat
     4501 gttattaccg gtaggcattg caatgggctt gcctgctcca ttattagttg gtttaatgcc
     4561 ggcaacttat gcttacttct ttattccgaa ttatccgtct gatatcgcaa ccgttaactt
     4621 tgacgtaacc ggtacaacta aaatcggtaa atattacttc aaccacagct ttatggctcc
     4681 gggtttagtt ggcgtaatta ctgcctgtat tgtcggtatc gccgttgcaa atgtgctaat
     4741 ttaattttaa caaaaaaaca agcggtcaga ttaagtaaat tttttaccaa atctgaccgc
     4801 ttgattattt actgcaaaac caattaggct ttgttaaaga gttttgcatc taacgagtat
     4861 tttccgccac cgataaaaat ataggttaag aataataccg aatacacgcc tgctaattca
     4921 ccgccgttta ataaaggtaa tagcacattg ccttgagctc cgtgagcaat aaagaacgcg
     4981 tatgccatca tacctgaaag gataaacgct gtttggcggg taaatagacc taaaataaag
     5041 aaaattccgc ccacaatttc caaaataccg cctacaccat aaatggaggc aaggggtaca
     5101 ctgccgtttc cgttggtcat cgacattgga aattcaaaaa atttcgctgt gccgtgtaat
     5161 aaaaacatat aaccggcaac aacacgggtg attaaaaacg ctaaaggttg taaatttgtt
     5221 aaagtagagt tcatttgctt tccttaaaat aaattgtgat ataaaggtca attgattgag
     5281 gcaggcattc tattttaaaa acctatactt cacaatccag ccgtcatgaa atagattatt
     5341 taccattaat aaacaattag gctctcgcta acacccaaac ctgaatttct actactccag
     5401 ctttgagtaa ctcggcacaa attacattta aggtggaacc tgtggtgagc acatcatcaa
     5461 caatcgccac tcgtttatag gcttttaacg gttgatagaa aaatgcccca cgcagatttt
     5521 tccgccgttc agcagcggtc aattctcttt gtgagacggt tgctctcaca cgttgtaaac
     5581 tctgggtatc cagcggaatc tgtaaccact tcgataacca atcacacaat aattcgcttt
     5641 gattaaaccc tcttttccaa tgtctgtgcc aaaaaagtgg tacaggcata atcacttcag
     5701 gcaagctcag aacctgttcc ctttgagctt gtttgattgc cagcagcaat tgtcgggcaa
     5761 gggcttgatc ccaccaatat tgctgctgga acttaaattg atgtacccaa tgtgaaagtg
     5821 gcgctcggta ttcatttacc ctcactaatt tatgccactt cggttcattt ttcaaacaat
     5881 agccacaagc gtttcgaaac tccaccgcac tcattccaca atgacgacaa taaggggttg
     5941 gttttagcaa tttttggcaa tggctgcaaa tgccttgcac gccaatagcg agtggatgat
     6001 tacaatgaaa gcaacggaag ccgaataaat tcacatttcc tccttttatt tgcagattat
     6061 caaatttagc cttatttttt ccggctattt ttgtgacatc gctcgaaaaa agagtaaaat
     6121 ctcattatca gtaaataaaa aggaaaccga gatgaaatcc ctccaaaaac cgatcaaaat
     6181 tgtctttttc gatattgatg acaccctcta tgtaaaacat aaagcctata ttccaaaatc
     6241 aattaccgag caggtattac ctcgtttaaa agccaaaggc attattccgg ccattgccac
     6301 cggcagaaat cacggctgtt ttcctgaagc tctcaaacct tatttaaacc ctgaaatcgg
     6361 ttttgagctg tttgttacca ttaacgggca atataatttc tataaagatc aaataattag
     6421 tgattattcg ttaagccgtg agcaaattga acgtattatc gaaaaagcga atgctttggg
     6481 tattctgtat gcctttatta cgccaatgac gattgcagtt tccgaaacca atccgattgt
     6541 gcacgaagcg gttttaccga tcacaccgaa ttatttgatc gatccacact actacgaaaa
     6601 agagaaaatt gtgcaaatgt tactgttcta caccgaagag cagaaacaag cggtcgaaaa
     6661 tagtggcatt tttgcaaatg atttaaaaga ggctcgctgg caccccaatg cggtagatat
     6721 tctgcgaaaa gaaaattcga aagctcgtgg cattcaagat gtattagcaa ccttgaatat
     6781 tgagattgaa agtgcgatgg catttggcga tggtattaac gacttggaaa tgctcccgac
     6841 cgtaggtgtt ggtgtggcaa tgggtaatgg cgtgccggaa ttaaaagcgg tggcagattt
     6901 catcaccaag ccgcttgagg aagacggcat tctatacgct cttgaaactc ttgaggtgat
     6961 ttaaaagcaa gcggttgtaa gtttttctcc atttacaacc gcttgtagtt tctaattacg
     7021 cctaaatagc ttatcgcctg caatcgctaa aatcgcccca ctgacgacca ccaacgcccc
     7081 aagataactg atccaattca tatccggctt ggcaaaaata tgcggataaa aatagtagct
     7141 aaggttggaa aagatcatcg taaaaatcgg caacatcgtg gttacaatgc taacctttga
     7201 agtatcccaa tgattcaacg cctcaccgta tgaaccgtag ccaattaagg tgttcaacag
     7261 caataaataa agcaacccca cagaaaacca tcctcaagat tgcctaactg tgccggtgag
     7321 gcaaacggta acatcagcag agaacagccg atgtaaataa taaacagcac ttgctgagag
     7381 ttaaatttcg ctaataataa tttttgagcc acgccgtata gcacccaaat caagctggct
     7441 ccggcactca ataacactcc gagagcgtag gtgttgagct gtaaaatttc agcaaattta
     7501 tcgttaaaaa atgcgacaag ccctgcaatc aataacacaa atccaatctt ttgaaacaag
     7561 ccgaactttt ccttaaacaa taatacgcct agtagaatca tcgaaaatgg agctaactgc
     7621 cacaatactt ggttaatggt gggcgaaaca tagtgtagag cctctgcaaa caaaaagaaa
     7681 tttgccgaca gaccacacac ccctaacaaa attaaaccga gttctttttt gctacaagcg
     7741 gtcaatttgg gtaaacgttt tgcaaatcct agaatcagca atagccctac tgatgccaca
     7801 acgaacctaa cccaaactaa agtttgggca tctatcactt tcaatacttg ctgggcggca
     7861 atcggtagcg ttccccaggt cattgctgcg gttaatgcaa gtgaaaagcc aagtaagggg
     7921 cgttgtttca tcgtggaaat tcctttttct attctatgtt tctatatttt agaaagattg
     7981 agatacaaaa aaaccccgcc attggcgagg tctcttattg attttgaaat gattagtcag
     8041 caatgatgct tacatattta cggcttttct cgcctttcac ttcaaattta actttaccgt
     8101 cagcagttgc aaataaagtg tggtctttac ccatacctac atttgaacct gcgtggaatt
     8161 tagtaccacg ttgacgaacg ataatgctac ctgctaatac agattcacca ccgaaacgtt
     8221 taacaccaag gcgtttagct tcagaatcac gaccgttacg agttgaacca ccagcttttt
     8281 tagttgccat cttctagatc tcctctgaaa ttatgcttga atcccagtga ttttcacttc
     8341 tgtgaaccac tgacgatgac cttgttgttt acggctgtgt ttacgacgac ggaacttaac
     8401 gattttaact ttctcgccac gaccttgtgc tacaacttca gccactactt ttgcacctgc
     8461 tacaaccggt gcaccgattt taacatcttc accgttaacg accattaaca ctgagtcaaa
     8521 ttcaactttc tcaccagttg caagttcaag tttttctaaa cgaactactt gaccttcact
     8581 tactcggtgt tgtttgccgc cactttggaa aactgcgtac atatttactc cgctatgtgc
     8641 caactgcttt tgcttttagg cactaaatta attcataaaa atagggttgc aattctacgg
     8701 aaaaagattc gaaagagcaa gagcgatttt gagaattttt tgaaatttgc aaaaaatggt
     8761 aaaaatacta ccgcttgttt caactgcgac taaaaaacaa tagactgctc cgattttaca
     8821 acaaaatcat aattcttttt tgaacacagg ctaaattaag gtagaatcta ctcaattttc
     8881 gtcttacttt atggattcga tggctgtgca acttttttct ttagaacaaa ttcaagccct
     8941 tgcgaatgaa gatatgcaag cggtaaatgc cgaaatttta gctcaactta attccgaagt
     9001 gctactgatt aaccagctcg gacattatat catcgcaggc ggtgggaaac gtattcgccc
     9061 gttaattgcg gtgcttgcgg ctcgtgcctt gggttatcgt ggtaaaaagc atatcacggc
     9121 ggctgctttt attgagtttg tgcatactgc tacacttttg cacgatgatg tagtcgatga
     9181 atccgatatg cgtcgtggca gaccaaccgc aaacgcccat tttggcaatg cggctagtgt
     9241 attggtaggg gatttcatct atacccgttc gttccagatg atgacggatt tagactctct
     9301 gcgtgtgtta aaagtgatgt ctgccgccac caatgcgttg gcagaagggg aagttcagca
     9361 attaatgaac gtgaacgacc caaacaccac tgaagcgaac tatatgcggg tcatttacaa
     9421 taaaaccgcc cgcttgtttg aagccgccac tcaatgcgtg gcgattgcgg ctgatgcttc
     9481 tgaggaaatt gaaaccgcat taaaaaatta cggttgctac ttaggcacgg cattccagct
     9541 gattgacgat attttagact actccgctca aagcgaaagt ttaggcaaaa acgtaggcga
     9601 tgactttgtg gaaggtaagc caaccttgcc attgctccac gcaatgcacg ctgccgacaa
     9661 agcaggcaaa actactcaag cagagctgat tcgcaatgct atcgaaaacg gcggcgaatt
     9721 ggaacaaatt ccacaagtat tggcaattat gcaagaacat caatctctgg attatgccat
     9781 gcaaaaagcc cgtgaagaag cccaaaaagc ggtggattca ttaggggttt taccggaaag
     9841 cgagtacaaa caagccttgg tttcattggc gtatttatcg gtggatcgct cttattaatt
     9901 tgcaaaaaat catataaatt taaccacttg taggggcaaa tgatatttgc cccataacct
     9961 ataattcttt tgggcaaatg ttatttgccc caacataaca atgactgaac aaaaccaacg
    10021 accccaagaa catattgaaa gcgtacgagc gagacgccgt gctcaaaatc agaaaaaagg
    10081 cccgaatgcc ccgtttgtgc gtgagaaatt aacgctgcca aacggacata acaaattact
    10141 gctgcactct tgctgtgccc cttgctcggg tgaggtaatg gaggctattc acgcctccgg
    10201 cattgagttt acgatctatt tttataaccc gaacattcac ccgctgaaag agtatttgat
    10261 ccgcaaggaa gagaatattc gctttgccga aaagtggggt attccgttta tcgatttaga
    10321 cgatgactac gaaaacgacc gcaaagagtg gttcaaaaaa gccaaaggaa tggaatggga
    10381 gccggaacga ggcattcgtt gcacaatgtg ctttgatatg cggtttgaaa aagcagcgga
    10441 atacgctcac aacaacggtt tccctgtatt taccagttgc ttaggcattt cccgatggaa
    10501 agatatgaac caaatcaacg actgtggtca ccgtgcagca gccaaatttg atgacgtggt
    10561 ttactgggac tacaactggc gtaaaggcgg cggctcacaa cgaatgatcg aaatcagcaa
    10621 acgtgaacgt ttctaccagc aagaatactg cggttgcgtc tattcgttaa gagatagcaa
    10681 caaatggcgg ttggaaacag ggcgtcagcg gattgaaatc ggtaagctct attactcacc
    10741 cgattaaatg ttacttttct ttgcttcgcc aaagaaaagt aaccaaaaga aaggcgaccc
    10801 gaacttcgct tagttacgat ttccttaacg gaaaatttgt aaactcgcta cgctcaaaca
    10861 gtacaaattt tcctagaaat cgcaagtcgc tcagacggga caagcggtac attattcaaa
    10921 attttttaca aaatatagtg atgtactctt cccttctgaa ccgcccgaac gtgtttgaat
    10981 tggtaggaaa atttactctg agcgaagcga atgtaaattt tccgtgaaac caattcaaac
    11041 taagtgagga taagcggtga agtaggggtg tttctttggt tactttcttt gcacaagcaa
    11101 agaaagtaac gaaaactcag atatagattt caacttaaaa atttacagaa ggaataataa
    11161 caaatgtcaa aattcgctat ggttttcccc ggtcaaggct cacaagcggt tggaatgctt
    11221 gccgaccttg cgccacaata tccaatcgtg gaagaaacct tcaaacaagc aagtgatgtg
    11281 cttggctatg acttatggga tttagtgcaa aacggtactg ccgaagagtt agggcaaacc
    11341 caccgcactc aacctgcgtt gcttgcctca agcgtggcac ttttccgcat ttggcaacaa
    11401 aaattccctg agcaaaaacc agccgtgatg gcagggcata gtttgggtga atactcagcg
    11461 ttggtgtgtg ccggtgtgtt ggatttccaa gatgcgatta aattggtcga gttgcgtggt
    11521 cgtgcaatgc aagaagccgt gccggcaggc agcggtgcga tgtttgcgat tatcggttta
    11581 gataacgaaa gcattattaa agcgtgcgag caagcagcaa gcgagaccgg tgaaattgtc
    11641 tctgcggtaa actttaacag cccggggcaa gttgtgattg ccggcacaaa agcagcggca
    11701 gaaaaagcag gagaattgtg taaagccgca ggtgcaaaac gtgcgttacc attagcggtg
    11761 agcgtacctt cacactgtgc tttaatgaaa ccggcggcag aaaaattagc cgaagccttg
    11821 caaaatattg agctaaatca accgcttgtc cctgttatca acaacgtgga tgtggcagtg
    11881 gaaacagatg ccgataccat tcgtaatgca cttgttcgcc aactttacag ccctgttcgt
    11941 tggacggaaa ccgttgaaaa aatggcacaa gatggcatca ctactttata tgaaatcggg
    12001 ccaaacaaag tcctaaccgg tttagcaggt cgtattgtaa aagaattatc agccaaagcg
    12061 gtaaatgatg ttgccagctt tgaggcggtt gaggcttaat tttcgggagc gtaaaatgag
    12121 taaagaaacc ctatttatgc taataagtgc attgattgcc ttagtgattt tacctttagc
    12181 ttatactgtt ggtatcgcta aagaaacatt aggcggaaat atgggaatgt tatccattgc
    12241 tgccggttgc tcatttttag cctatttttt aagtctaaac ccgaaaattc gccacgcctt
    12301 tatcggaggt tcagctatta cagctgctgc tattcaagct tgggtattat atggtgtgct
    12361 attaagcgaa aatgacccca aaagtgcggt cgttttattt caaatttatc tgtttagtag
    12421 tttagggcca ctgttttttt cagctatttg ggcatttact cgcccacgtt ggatttaccc
    12481 taaacaaggt agtgcggtga aatatgcctt atttggtggc ttaacgaccg tgataaacta
    12541 cttgattacc ctttttattt tttatattct gacgttataa cgtcatacta aggagaattt
    12601 atgagcaaaa tcgcattagt tacaggggca acccgtggaa tcggcaaagc gattgccgaa
    12661 gaattggtag caaaaggcta catcgtagtc ggcaccgcaa cctctgaaaa aggggcggaa
    12721 tctatttcag cttatttagg cgagaacggc aaaggcttag tgctaaatgt tgccgatgca
    12781 gagtcgattg agagcgtatt aaagcagatc aaagagacct ttggcgatgt ggacgtgtta
    12841 gtcaataacg ccggcattac ccgtgacggc ttattaatgc gtatgaaaga tgaggattgg
    12901 ttcgacatca ttcaaactaa cttaacttcg gtttatcgtt tatccaaagc ggtattacgc
    12961 ccgatgatga aaaaaggcgg acgcattatc acgatcggtt ctgtggtcgg ctcaatgggc
    13021 aacccggggc aaaccaacta ctgtgctgcc aaagcaggct tagttggttt ttctaaatct
    13081 ttagcgaaag aagtagcaag ccgcggcatt acggtaaacg tggttgctcc gggctttatc
    13141 gccactgata tgaccgatga gctaaccgaa gatcagaaaa atgcgatttt aagccaaatt
    13201 cctgccggtg aactcggttc tccacaagat attgccaaag cagtcgcttt cttagccagc
    13261 gatgatgccc gttatatcaa cggtgaaacc attcacgtta atggcggttt atatatgagc
    13321 taatccgcta caagcggtct tttttcaacc gagctttgta aattcagagt tcggttttgt
    13381 tatttgttat tttgtgttat cacctgcttt ataggtatag gtgaaaaagt gtgaatatgt
    13441 tatgactaga aatacattag tagtaggctt tttattgttt gctattttct ttggtgccgg
    13501 caatctgatt ttcccaccaa aattaggtta tgaaagtagt acagaatttt tctcagccat
    13561 ttcaggcttt gttttaactg gagtagggct tcctctactc ggtattatcg tcagtgcatt
    13621 ttataatgga ggctataaat ctgcattaga caaaatccac ccttggtttt ctttagcatt
    13681 cctaacttct atctatttag cgatcggtcc attctttgcc ggtccgcgca cggcagcaac
    13741 tgcctatgaa atggcgataa tgccgttttt aggggattcc agccaagtgt caatgttaat
    13801 ttttacctta atttactttg cctttaccct atggttagcg ctaaatccaa ataaaatggt
    13861 tgatcgcatt ggtgcaattc ttaccccgat cttgcttata accatcattg gattagttat
    13921 tcgagccggc tttattttag ataccgatcc acaaatcact acgcctaata cgaatgaaca
    13981 atcttacttt tttaaaggta tggttgatgg ctatctaact atggatgcac ttgcttcgtt
    14041 agcctattcg gtaattgtat taaatgctat tcgtgggaaa ggggtaagcc aagccgcatt
    14101 acaacgccaa acagtttctg ccggcttagt tgcagcagtt gctctagctg taatttatat
    14161 ttcattgggt tggattggta ataatatgcc aattagtgca aacacacttt cgatgttatc
    14221 agaaagaaaa ttggatctag gtacttacat tcttaacact gtgactaccc aagccttcgg
    14281 tgaactcgga cgtagtgtct taggagtgat tgtttcactt gcctgtttaa ccactgctgt
    14341 cggtttagcc gtatcagtca gtgaatactt taatgaaatt ttcccgaaaa tttcatataa
    14401 agtttatgct atcttattta ctgtgattag ttttactgtt gctaaccaag gattaaaaga
    14461 agttattagc aaatccattc cggtattatt agttctctac ccaatagcaa tgactgtcat
    14521 tttaatgttg ctgattaatt tagtgattaa acttccgttg tctgcacaac gtctatcact
    14581 cctattagtg acaattgtag cgatagcttc agttgccggt gtcgggttta ccgaaaactt
    14641 gcctctaaaa tcctactcga tggaatggct acctttcgct atcattggag ctattattgg
    14701 agcaattatc cacccgctta ccggtcgaaa gcataaataa agtaaaggca gtgagcattc
    14761 actgcctctt ttttaggaga gaataatgcc tgaattaccc gaagttgaaa ccagcgttcg
    14821 tggtgtatcg ccctatttgg ttgggcaaac tattgaacaa atcatcgttc gtcagccaaa
    14881 gttacgttgg acggtgagtc ctgaattaag ccaaatggca ggggcaaaaa ttaccgcagt
    14941 agaaagacgt gccaaatatc tgattatccg caccgacaaa ggcgatattt tggtgcatct
    15001 gggaatgtcc ggctcgctcg ggattttgca agaacgtcaa caaaaagaaa ttggcaaaca
    15061 cgaccacgtc gatcttatta cccaaaacgg cacaatttta cgctataacg accctcgcaa
    15121 attcggctgt tggctatggg cagaaaaagc agaagatcac gaactgttgc gtaaactcgg
    15181 gcctgaaccg ctttcaaacg atttcacagc agattactta tttgcaaaaa gccgcaaaaa
    15241 aacgaccgct tgtaaaacct ttattatgac caatgaagtg gttgttgggg tggggaatat
    15301 ctatgcgtgc gaaagtttat ttatggcagg cattcaccct gagcttgcag cacaaaacct
    15361 gactaaaaaa cagtgcgaac gtttagtaaa aaccatcaaa gaagtgctga ctaaagcgat
    15421 tattcaaggc ggcacaacac tgaaagattt tatccaacct gacggcaaac caggctattt
    15481 tgctcaagtg ttgcaagtgt atggaagaaa aggggaaacc tgtaatgact gcggaaagcc
    15541 gattgaaact aaagtaatcg ggcaacgtaa tacctatttt tgcccgaagt gtcagagatt
    15601 gccgaagtaa tctaataata aactctctcc ccctgaggga gagagacagc ttgttgaaac
    15661 gttaagtgaa acaagcagag agagggtaaa acctaaacct gcaaccaaaa ggtaacaggc
    15721 ccgtcatttt gtaggctgac ttgcatatcg gctgcaaatt gcccggtttg ggtatgaatc
    15781 ttagtggcgg cttgttgatg aaagtaggca tacaaacgtt cggcatcaac cgggcttgct
    15841 ccttttgaga aactaggacg caagccttta ttggtatccg ccgcaagggt aaactgtgaa
    15901 accactaaca aactgccacc tgcttgctgt acgtttaagt tcattttccc ctcgctatcc
    15961 gaaaacaccc gataattcag cactttttcc agtagcttat ctgctttcgc ttcatcatcg
    16021 tctttttcca caccgagcag caccaacaaa ccgcttgcaa tttcgccaac gacttgccct
    16081 tcaacctcca cttttgcgaa tttaacccgc tgaattaagc ctatcattaa tgctcccctt
    16141 gtctgtttcc atttcgtcta aaaccgccgc aaattgcccg ccaagtaata ccacctgcca
    16201 acttaactga atccataaca gcataatcgg taacaccgct aatgctccat aaattgcctg
    16261 ataagacggg aaggcagtaa tataccaaat aaaggctttc ttccctaaag taaagaaaat
    16321 cgctgccatt aatgccccga tagcagcgtg ttgaaatttt acctttctaa ttggaataaa
    16381 tttatacact aaggtaaaaa gtagccaaat tagcaaaaac ggcatcaatt ctaataaatg
    16441 ctgcccgagc gataacgctc catttccatt aaaaatagaa agcgacatta cataggtgct
    16501 aatcgcaatg ctagcaccgg caagcagcgg gccaaacagc aaaatgagaa agtaaagcaa
    16561 gaaagagaac cacatcggac gctgacgatt atcgtgccaa atggtattca atgcctcatc
    16621 aatactctta atcagcataa tagtaaccac aaataagccg ataaaactta ccagccccat
    16681 ttgtttggca ttactgacaa acaactcaat gtattccgac accatctcgc tcgcactcgg
    16741 ggcaaagttg tcataaatga acgcttttaa ctgctcgttt gcttcgtgga aaatcggaaa
    16801 aaaggtaaaa atggaaaaca gcacgacaat aaacggcact atcgccagca ttgtgctata
    16861 agtgagataa ccggctgaaa tcgcaatgtt attttcattg taacgcttgg caaagagctt
    16921 aataaatctc cacagtcgat ttaacatctt aacctctcac caaataatcg ccttcacaca
    16981 cccatttgta ggtggttaag gcttctaatc ccatcgggcc tcgtgcgtgt aatttctgcg
    17041 tgctaactgc cacttctgcc cctaaaccaa actcaccacc atcggtaaaa cgggtgctgg
    17101 cattaatata aaccgccgcg gcatcaacct gttgtacaaa ttgacgggca agttgataat
    17161 cggaggttaa aatcccctct gaatgctgcg aaccataatg gcggatatgc tctaccgctt
    17221 gttgcaggtc attcaccacc accacgttaa gatcaagcga taaccactct tggctcaatt
    17281 ttgcttgatc aagcggttca attccttcaa ttttttgcaa atcgttactg tgaatagtaa
    17341 cgccaagctg ttgcattttg gcgaccaatt tcggcaaaaa ttccaccgca atcgcttttt
    17401 ccaccaacag cgtttccagc gtattgcaag tgcttgggcg ttgcgttttg gcattagcga
    17461 tgacctctag agatttctcc tgatccgctg ttttttccac aaacagatga caaaccccaa
    17521 tcccccccac aatcaccgga atggtcgaat tttctttaca gaattgatgt aaacctgcac
    17581 cgcctcgagg gatcaccata tcaatgtagc gatctagctt caatagttcc aataataagg
    17641 ctcgatccgg atccgtcacc gcttgcactg ccaatttcgg caagccggct ttttctaacg
    17701 catcttgcac tacatcaacc aacacagcat tggtaaattt ggtctcttta ccgccacgca
    17761 aaatcactgc attgccggtt tttaggcata atgatgccac atcaatggtc acattcgggc
    17821 gagcttcgta aatggttaaa atcaccccga gcggcacacg ctggcgttcg attttcaaac
    17881 cgctgtctaa cacgccaccg tccatcacct gccccaccgg atccgctaat ttggcaacat
    17941 ttcgcacatc gttagcaatg tcttttagcc gatcttcatt caataataag cggtcaataa
    18001 tcgccaatga aatcccttgt gcttgagcaa attcaatatc tttggcatta gcgtataaaa
    18061 tttcttctgc acgttgctca aggctatcgg caatcgtgag taaggcttgg tttttctgta
    18121 ggttgccaaa ttgagccagc tcaaaactag cttgtttggc ttgctgggca agttctatca
    18181 tctgcataaa ttaggtctca tttaatcaat attctccata atctaacacg ttcttttatg
    18241 tgaagaaata tgcaaaagcc aagaaaattg gataaaatgg gggaatttta aaacattacg
    18301 caattcccct ctccctgatt tttctcctga acggagaaaa atctatccct ctcctacaag
    18361 gggagagggt agaatttaac tctctcccct tgtgggagag agaccgccta cagattcgtt
    18421 cagaatctgt aggcagagag aggggtaaaa acagataaaa cttaaacaaa atgataaaca
    18481 ctacccctaa acagctaatc cacattcttc cgcctcaact ggcaaaccaa attgctgccg
    18541 gcgaggtggt ggaacgccct gcgtctgtgg taaaggagct agtggaaaac agtttagatg
    18601 ccggagccaa tactattcaa atcgacattg aaaaaggcgg ctctcagctt atccgcattc
    18661 gagataacgg ttgtggtatc agcaagcaag atttaccgct ggcacttgct cgccacgcta
    18721 cgagcaaaat cagcagcttg gaagatttag aagccatttt aagtttaggc tttcggggcg
    18781 aagcattggc aagtatcagc tcggtttcac gtttaacact gacctctcgt accgcagagc
    18841 aaaccgaagc atggcaagcc tatgcacaag ggcgggaaat ggcggtggaa attcagcctg
    18901 cctcacaccc gattggcaca accattgaag tagcaaatct gttttttaac acaccggcac
    18961 ggcgtaaatt tctacgcacc gataaaaccg aatttgccca tattgacgag gtggttcgcc
    19021 gcattgccct tgccaagccg aatatcagtt tcagcttaag ccataatggt aaaatcgtcc
    19081 gccaatatcg caaagtgcaa gacaattcgg tagagcagca acaaaaacgg gttgcagcca
    19141 tttgcggtga tgagtttatt caacattcaa cttatattga caggcaacac ggggatctgc
    19201 acctacacgg ctgggttggc tcaccggctc ttgcccgtct gcaaaacgac ctttgctaca
    19261 gctatgtgaa cgggcgaatg atgcgggata aaaccatcaa tcacgccatt cgccaagcct
    19321 acggcgacag cattgcaagt ggttactatc cggcttttgt gatctttttg gatttagatc
    19381 cgacaatggt tgatgtaaac gttcacccgg ctaaacacga ggtgcgcttt catcaaggaa
    19441 gactggtaca cgactttatt ttgcaaggcg tactcaacag cctgcaacaa acccaaccta
    19501 cgcttgaact gcgaaatgaa gtgaacgagc cgttaccgag ctatgttcaa gacactaacc
    19561 gccaagctgc ggggcaaaat atttttgtgt cgcaaaccgc tccacaagcg gccgaattta
    19621 cgcaaaaaaa tgcaagtcga tcaagctata cgcctaaatt tgaacgaagc aacagtgtga
    19681 gttcgtccgc tcaaaaatgg tataaggaat tggttggtgg cgatttgcac caaacaacgc
    19741 caactatcaa aacgcaaagc acgcttgttg agccaaaaac agaaccccaa actgaacata
    19801 aaacagaagc cgtttcgcaa aaaattacgc aaattgcacc gcttgcaagc caccattcac
    19861 aagccttagc gattgtaaaa aaccaagcgg tgttaattaa agaagaggaa aatttttacc
    19921 tgcttccact ggcaacatta gccgagctta aactcaagct acaactggaa aaagggcaat
    19981 ctcaagcgtt gttgatccca ctgaatctca gtttagatca acaacaaacc gaacgctggc
    20041 aacaaaccaa agaagaacta actaagcttg gcttttcgat cagcgaaaaa cagtggcaag
    20101 cccaaacacg gctcacagtg ctttccgtac cacaggctgt cagagaacaa aacctgcaac
    20161 agcttttatt tgctctgttc aaccaaacac aagcggtcaa tttagccgaa ttttttgcaa
    20221 aaaaatgcga aaaacccacc gcttgtacgc taagtgacgt gattgctcta ctctcagaaa
    20281 ttgagcttta tgccaatggg aaaaaagagc tggagggaat aaggaaggaa gtggattttt
    20341 ccaagttttt atagtctaaa gggcaatacc ctttagccaa ataaatgaga gtggctgcca
    20401 attcgcagca gctctagctt atcatcttcc acttttcgat agataagaac caaatcaggt
    20461 tggatatggc aatccctaaa gcctttccat tcgccggtta aagcgtgatc gcagtatttc
    20521 tcaggtaatg gcttgtcatt aatcagaaaa tacaacgcct caattaatga agaggataaa
    20581 gactcaaccg cacatttttt atagtcacgt ttaaaagcat tactatactc aatctctcgc
    20641 attgagttgc tccattaaat ccgcaatact atttgctgaa tgtagctttt cctcgccatt
    20701 cttaacagcc cttaatactt ctgctgtctc aaaattaggc tgataatcaa actgaaaacg
    20761 tttttctgtt gcgattttcg ctaatgtcat tcgcatcaca tcagagacag tcaagccgat
    20821 accggctaac acctcacttg cttgctcttt aatttcaggg ctgattctta cgcttactaa
    20881 tgcactattt gccatcaaaa actc
//
LOCUS       DANLYI010000017        20747 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.017, whole genome shotgun sequence.
ACCESSION   DANLYI010000017 DANLYI010000000
VERSION     DANLYI010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 20747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20747
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.017"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            complement(101..1450)
                     /locus_tag="PXD81_000506"
     CDS             complement(101..1450)
                     /locus_tag="PXD81_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HDL5536570.1"
                     /translation="MLLFIMFAAIILLLVLIMKFKVHAFVALIIVSLLTALAAGIPVD
                     KILPTLLNGFGSTLASVALLVGLGAMIGRLLEITGGAKVLADTLINKFGKERAPFALG
                     VASLLFGFPIFFDAGLVVMLPIIFSVAKQFGGSVLRYAFPSAGAFAVMHAFLPPHPGP
                     VASGDLLGVNMGLLVLVGLVCAIPTWYIGTYLFSQFISKRIHVDLPKAFLNGLSANEM
                     AVQNPPSFGKVLTVLVLPIILILFDTGLNTLSVAKVIDGSEMWVQSLRLIGKTPVALL
                     ITLIVAIMLLRDNRSFDQIEKICNNALGPICSIVLVTGAGGMFGGVLRASGIGDVLSE
                     MMKDTGMPIVVAAFIIATVFRVAQGSATVALTTTAALIAPTVAAATDLSQFDLCFIVI
                     AIASGATVLSHVNDSGFWLMSRFLEMDEKTTLKTWTMLETAIGLTGFACALIGSWLL"
     gene            1637..2152
                     /locus_tag="PXD81_000507"
     CDS             1637..2152
                     /locus_tag="PXD81_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HDL5536571.1"
                     /translation="MANGKAFILMGVSSTGKTSVGTAVAQRLGIKLIDGDDLHPRANI
                     LKMASGTPLNDADRTPWLERINDAVFSFEQKSEKGIIVCSALKKKYRDQIRQGNADVT
                     FLFLHGSFELVLARMQQRKGHFMKPEMLQSQFNTLEIPQADEPDVIFIDIDGSFDEVV
                     ERCVKAVEPLL"
     gene            complement(2206..3204)
                     /gene="gntR"
                     /locus_tag="PXD81_000508"
     CDS             complement(2206..3204)
                     /gene="gntR"
                     /locus_tag="PXD81_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015674159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HDL5536572.1"
                     /translation="MRKPKRPTLQDIATHLGITKMTVSRFLRDPNTVAKETGERIAQA
                     IELFGYIPNRAPDILSNAKSRAIGVLVPSLTNQVFADVIKGIEQITDQASYQTMLAHY
                     GYSEQKEEQRIESLLSYNIDGIILSENHHSPRTLKMLEVANIPVIEIMESGECGIQQA
                     VGFDNISAAQAMVETMIHRGCRQVVYFGARMDKRSQLKMQGYEQAMLKHGLSPHSITT
                     AEHSSFTLGASQLKQALQHIPQLDGIFCTNDDLAIGALFECQRLGIHVPQQIKIAGFH
                     GHDVGQSLTPQLASVITPRLEIGRVAAQELLNRIHGVPQQSSIINLGYQIHLGESV"
     gene            complement(3297..4457)
                     /locus_tag="PXD81_000509"
     CDS             complement(3297..4457)
                     /locus_tag="PXD81_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HDL5536573.1"
                     /translation="MIEQSYKIALLFNANKIYDRGVMQGIGQFIQASQCTWDIFVEDD
                     FVYHKNSIESLSIDGIIADFDDLETVELLKDIDVPIVAVGGSYQNPDYYPDLPYVATD
                     NYALVEAAFLHLKQKGINQFAFYGYPAETEKHWAIERKNAFIQLMEKYEHHPNCYIGD
                     QFHSDNWVELQAKLGEWLQTLPAHTGIIAVTDARARHLLQTCEHLKIAVPEQLCVIGI
                     DNEELIQYLSRVSLSSVTQGTSQIGYHAAKLLYRKLNNLPVSTKPILIPPVKVEERRS
                     TDYRSLQDPMVIQAMHFIRHRACQGIKVEQVLDHLKISRSNLEQRFKQEMNKTIHQVI
                     HEEKLARATYMLKFTEISTQEIANVCGYPSLQYFYAVFKKEFGKTPKEFRQE"
     gene            complement(4580..4966)
                     /gene="rplQ"
                     /locus_tag="PXD81_000510"
     CDS             complement(4580..4966)
                     /gene="rplQ"
                     /locus_tag="PXD81_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014992360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HDL5536574.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMASALVEHEIIKTTLPKAKEL
                     RRVVEPLITLAKADSVANRRLAFARTRNVDTVAKLFNELGPRFAQRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRPEVATEAAAE"
     gene            complement(5002..5991)
                     /gene="rpoA"
                     /locus_tag="PXD81_000511"
     CDS             complement(5002..5991)
                     /gene="rpoA"
                     /locus_tag="PXD81_000511"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HDL5536575.1"
                     /translation="MQGSVTEFLKPHLVNIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSSKEGVQEDILEVLLNLKGLAVKVQNKDDIILTL
                     NKSGIGPVTAADITHDGDVEIVNPDHVICHLTDKNASINMRIRVQRGRGYVPASARLH
                     TQNDDRPIGRLLVDARFSPVDRIAYNVEAARVEQRTDLDKLVIEMETNGTLDPEEAIR
                     RAATILAEQLAAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAETIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIAED"
     gene            complement(6022..6648)
                     /gene="rpsD"
                     /locus_tag="PXD81_000512"
     CDS             complement(6022..6648)
                     /gene="rpsD"
                     /locus_tag="PXD81_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HDL5536576.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIESKCRNRLDVAPGQHG
                     ARKPRLSDYGSQLREKQKVRRIYGILERQFRNYYTEANRLKGNTGENLLVLLEGRLDN
                     VVYRMGFAATRAEARQLVSHKSIVVNGRVVNIPSFQVSVDDVVAVREKSKKQARIKAS
                     LELATQREKPTWLEVDATKMEGVFKRRPERSDLSADINEHLIVELYSK"
     gene            complement(6677..7066)
                     /gene="rpsK"
                     /locus_tag="PXD81_000513"
     CDS             complement(6677..7066)
                     /gene="rpsK"
                     /locus_tag="PXD81_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HDL5536577.1"
                     /translation="MAKTPVRARKRVKKQIADGVAHIHASFNNTIVTITDRQGNALAW
                     ATAGGSGFRGSRKSTPFAAQVAAERCAEMVKEFGLKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            complement(7083..7439)
                     /gene="rpsM"
                     /locus_tag="PXD81_000514"
     CDS             complement(7083..7439)
                     /gene="rpsM"
                     /locus_tag="PXD81_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HDL5536578.1"
                     /translation="MARIAGINIPDQKHTVIALTAIYGIGKTRAKAICAATGIAEDVK
                     IRELSEEQIEKLREEVGKFTVEGDLRREVTLSIKRLLDLGCYRGLRHRRSLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            complement(7577..7690)
                     /gene="rpmJ"
                     /locus_tag="PXD81_000515"
     CDS             complement(7577..7690)
                     /gene="rpmJ"
                     /locus_tag="PXD81_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006232360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HDL5536579.1"
                     /translation="MKVRASVKRMCRNCKVIKREGVVRVICSDPKHKQRQG"
     gene            complement(7715..9034)
                     /gene="secY"
                     /locus_tag="PXD81_000516"
     CDS             complement(7715..9034)
                     /gene="secY"
                     /locus_tag="PXD81_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HDL5536580.1"
                     /translation="MAKQPGYQGSTQKGTGELKSRLLFVLGALIVFRIGSFIPVPGID
                     ASVLSELIRQQQGTIIDMFNMFSGGALSRASIFALGIMPYISASIIIQLLTAIHPPLA
                     ELKKEGEAGRRKISKYTRYGTLLLAFIQSIGISLALPNMVHGLVPNPSILFYVTSVVS
                     LVTGTMFLMWLGEQITERGIGNGISLIIFTGIVADLPATIGQTIEQARQGELSFIVLL
                     LVAIIAFAVTYFVVFVERGQRRIVVNYASRQQGRMMAPARTSHLPLKVNMAGVIPAIF
                     ASSIILFPASITQWFGQSEGFEWLFNLSQLLQPGQPLYVVLFTMAVIFFAFFYTGMQY
                     NPRDTADNLKKSGAFVPGYRPGEQTSRYIDKVMTRLTLIGALYIAFVCLVPYIITSLW
                     KVPFQLGGTSLLIVVVVIMDFIAQVQSHLMSQQYDSVLKKANLKGLN"
     gene            complement(9037..9471)
                     /gene="rplO"
                     /locus_tag="PXD81_000517"
     CDS             complement(9037..9471)
                     /gene="rplO"
                     /locus_tag="PXD81_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HDL5536581.1"
                     /translation="MRLNSLSPAEGAKHSAKRLGRGIGSGLGKTGGRGHKGQKSRTGG
                     GVRRGFEGGQMPLYRRLPKFGFTSLKSFHVAEIRLNDLAKVDGNEVTLETLKAANIIT
                     KDILSAKVILAGKIERAVVVKGLRVTKGAKAAIEAAGGSIEE"
     gene            complement(9476..9652)
                     /gene="rpmD"
                     /locus_tag="PXD81_000518"
     CDS             complement(9476..9652)
                     /gene="rpmD"
                     /locus_tag="PXD81_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HDL5536582.1"
                     /translation="MAKIKVTQTRSSIARLPKHKATLKGLGLRHIRHTVELEDTPAVR
                     GMINQVSYMVKVEE"
     gene            complement(9659..10159)
                     /gene="rpsE"
                     /locus_tag="PXD81_000519"
     CDS             complement(9659..10159)
                     /gene="rpsE"
                     /locus_tag="PXD81_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HDL5536583.1"
                     /translation="MSNIEKQAGELQEKLIAVNRVSKTVKGGRIMSFTALTVVGDGNG
                     RVGFGHGKAREVPAAIQKAMEKARRNMINVALNEGTLQHPVKGSHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVRNVLSKAYGSTNPINVVRATIDALENMKSPEMVAAKRGKT
                     VEEILG"
     gene            complement(10174..10527)
                     /gene="rplR"
                     /locus_tag="PXD81_000520"
     CDS             complement(10174..10527)
                     /gene="rplR"
                     /locus_tag="PXD81_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HDL5536584.1"
                     /translation="MDKKVARIRRASRARHLMREQGATRLVVHRTPRHIYAQVIAPNG
                     SEVLAAASTVEKVIKEQVKYTGNKEAAAVVGKIVAERAIAKGIKAVAFDRSGFKYHGR
                     VQSLADAAREAGLQF"
     gene            complement(10542..11075)
                     /gene="rplF"
                     /locus_tag="PXD81_000521"
     CDS             complement(10542..11075)
                     /gene="rplF"
                     /locus_tag="PXD81_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HDL5536585.1"
                     /translation="MSRVAKAPVNIPAGVEVKLNGQLLTVKGKNGELSREIHNAVEVK
                     QEADALTFAPREGIANADAQAGTARALVNNMVIGVTEGFTKKLQLVGVGYRAQMKGNA
                     VALSLGFSHPVEHTLPAGVTGECPSQTEIILKSADKQLIGQVAADIRAYRKPEPYKGK
                     GVRYSDEVVRTKEAKKK"
     gene            complement(11092..11484)
                     /gene="rpsH"
                     /locus_tag="PXD81_000522"
     CDS             complement(11092..11484)
                     /gene="rpsH"
                     /locus_tag="PXD81_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HDL5536586.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKVAISMPSSKLKVAIANVLAEEG
                     YIESVKVVEGVKPELEITLKYFQNKPVVESIQRVSRPGLRIYKRKDELPKVMGGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            complement(11521..11826)
                     /gene="rpsN"
                     /locus_tag="PXD81_000523"
     CDS             complement(11521..11826)
                     /gene="rpsN"
                     /locus_tag="PXD81_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HDL5536587.1"
                     /translation="MAKQSMIARDVKRAKLADKFYAKREELKKIISDVNASDEDRWAA
                     VLKLQTLPRDSSPIRQRNRCRQTGRPHGVLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            complement(11838..12377)
                     /gene="rplE"
                     /locus_tag="PXD81_000524"
     CDS             complement(11838..12377)
                     /gene="rplE"
                     /locus_tag="PXD81_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HDL5536588.1"
                     /translation="MAKLHDYYRDTVVNELKAKFNYSSVMQVPRIEKITLNMGVGEAL
                     TDKKLLDNAVADLAAISGQKPLVTKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLNAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTSA
                     KTDEEGQALLAAFNFPFRK"
     gene            complement(12395..12706)
                     /gene="rplX"
                     /locus_tag="PXD81_000525"
     CDS             complement(12395..12706)
                     /gene="rplX"
                     /locus_tag="PXD81_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HDL5536589.1"
                     /translation="MAAKIRQNDEVIVLTGKDKGKRGKVTKVLPNGKVFVEGINIITK
                     HEKPVPALGKAGGLVKKEAAIDVSNVAIFNPETNKADRVGFRFEDGKKVRFFKSNEKT
                     I"
     gene            complement(12717..13088)
                     /gene="rplN"
                     /locus_tag="PXD81_000526"
     CDS             complement(12717..13088)
                     /gene="rplN"
                     /locus_tag="PXD81_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012728338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HDL5536590.1"
                     /translation="MIQEQTMLDVADNSGARSVMCIKVLGGSHRRYAAIGDIIKVTVK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGAVIRFDGNACVILNNNTEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            complement(13311..15146)
                     /gene="glmS"
                     /locus_tag="PXD81_000527"
     CDS             complement(13311..15146)
                     /gene="glmS"
                     /locus_tag="PXD81_000527"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HDL5536591.1"
                     /translation="MCGIVGAVAQRDIAEILVDGLHRLEYRGYDSAGVAVLNADDKTM
                     QIVRRVGKVKALDEALDTKPLLGGTGIAHTRWATHGEPSETNAHPHRSGKIAVVHNGI
                     IENYEELKIVLQERGYVFQSQTDTEVIAHLVEWELRSASSLLEAVQKTVVQLRGAYGT
                     VVLNEEEPEHLIVARSGSPLVIGYGVGENFLASDPLALLSVTRRFAYLEEGDVAEITR
                     RTVDIYDREGNKAEREIHEGNFEADAADKGQYRHYMQKEIFEQPVAIMNTLDGRIKDG
                     KVDISAIAPNAAEILSKVEHVQIVACGTSYNAGMVSRYWFESIAGVSCDVEIASEFRY
                     RKFVTRPNSLLITLSQSGETADTLAALRLAKESGYMAAMTVCNVASSSLVRESDFAFL
                     TKAGVEIGVASTKAFTTQLTCLLLLNVAIGRLQGRMSEEQEHHIVQSLQRLPAQIESA
                     LVFDKQIEKLSEDFADKHHTLFLGRGEYYPVAMESALKLKEISYIHAEAYAAGELKHG
                     PLALIDSDMPVVVVAPENDLLEKVKSNIEEVRARGGQLYVFADHDAGFEEAEGFKTIV
                     MPKVDEVTAPIFYTVPLQLLSYHIALIKGTDVDQPRNLAKAVTVE"
     gene            complement(15194..15955)
                     /locus_tag="PXD81_000528"
     CDS             complement(15194..15955)
                     /locus_tag="PXD81_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HDL5536592.1"
                     /translation="MSKRNTQQRRHLIVQMVQEKNEVSVDELAHFFETSEVTIRKDLT
                     ALEESGFLLRKYGGAVKIPSEMMEEAFIEQLSIQKKMIAQAARDCIRDHNRIIIDSGS
                     TTGALVKALNQTGLVVMTNSLNLATELTSLECEPTVLMTGGTWDSRSESFQGKVAEQV
                     LRSYDFDQLFIGADGLDLARGTTTFNELVGLSQVMAEVSREVIVLIESQKIGRKMPNI
                     ELKWQNITKIITDSQLSFETKEQIEKMGVEVVIAK"
     gene            complement(16112..16312)
                     /locus_tag="PXD81_000529"
     CDS             complement(16112..16312)
                     /locus_tag="PXD81_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HDL5536593.1"
                     /translation="MKSILKVVGAMVLMVAVSGCNNMTKTQRNTAIGAAIGGVAGHAI
                     GESTGATLGGAALGGVIGSQIK"
     gene            16537..17571
                     /locus_tag="PXD81_000530"
     CDS             16537..17571
                     /locus_tag="PXD81_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL5536594.1"
                     /translation="MKLKKLSLALSTLLLGSAFANSALAEGRLTVYCSATNELCEQEV
                     QAFGKKYNVKVAFVRNGSGSTLAKIEAEKNNPQADVWYGGTLDPHSQAAEMGLLEPYK
                     SPNLEQIIEKFREPAKLKGNYSSAVYMGILGYGVNLDRIKKLGIEKVPSTWEDLLDPR
                     LAGEIQIADPQSSGTAYTAIATFVQLLGEEKAFDYFRKLHKNISQYTKSGITPARNTA
                     RGETAIGIGFLHDYAIEKKNGANIEMTAPTDGTGYELGGVSILKGARNLDNAKLFVDW
                     SLSKEAQELSWQKGNAFQVLTNTTAESSPYALDPKNLNLINYDFEKYGSSEERKRLID
                     KWVNEVKLAK"
     gene            17639..19675
                     /locus_tag="PXD81_000531"
     CDS             17639..19675
                     /locus_tag="PXD81_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016534092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HDL5536595.1"
                     /translation="MQNFSFSRPLVWVLIGLLGFLLLPSKALDYGLFDSTSDELLAAM
                     GWASLNITWAWFLPLLVFLLPNSTACKTRAKLELVGLGVLFAFIFLSAIVLKISLGYA
                     VLPLIIAIMGLATPNLATLKVMQGDRFIIGSLLSIILLIFFFIVYPTIAIFVSMFYNG
                     SEFVPSQVLTILQQPYVVRVIGNSLSVAGTVGVLSTLFGLAFALYTTRIAKRSAFIGR
                     IFSILPIVTPPFVVGLGVTLMLGRSGYVTEFLVDYFGFANNWLYGFTGIVIAHTLALT
                     PMSFMILEGALKSIHPSVEEAAYTLRSNRYQAFFNIIFPLLKPALANSFLVVAIQSLA
                     DFSTPLVLGGSFDVIATQIYFYIAGSQLDYASASTLGAILLVFSLAIFVIQYLWIGNR
                     SYVTVSGKSYRGDVQDLPSGMKWLIICTLLFWGTFNLVLYGSIFYGSFTVNWGVDYTL
                     TLDNYIKLFGQGFSDGGFPSLIQTVIFAAAAAPITALFGLLIAYVTVRRDFKGKKTLE
                     FLTLLCFAVPGTVAGVSYILAFNDAPIYLTGTGMIIILSMVMRNMPVGMRAAIAGLGQ
                     LDKSLDEASLSLKAGSFKTMLFVVLPLLKPALLSALVTSFVRAMTTVSAIVFLVTADT
                     RVATSYILNRVEDGEYGIAIAYGSILIVVMMAIILIFDWIVGDTRIARSKAKKM"
     gene            19773..>20747
                     /gene="fbpC"
                     /locus_tag="PXD81_000532"
     CDS             19773..>20747
                     /gene="fbpC"
                     /locus_tag="PXD81_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005700953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric ABC transporter ATP-binding protein"
                     /protein_id="HDL5536596.1"
                     /translation="MNNDFLVLKNVTKSFGKVTVIDNLNLSIPQGKMVTLLGPSGCGK
                     TTVLRLVAGLENPTSGQIFIDGEDVTQSSIQHRDICIVFQSYALFPHMSIGDNVGYGL
                     KMQGVPKEERLQRVKEALELVDLGGFEDRFVDQISGGQQQRVALARALILKPKVLLFD
                     EPLSNLDANLRRSMREKIRELQQRLNITSLYVTHDQSEAFAVSDEVIVMNKGKIMQKA
                     PAKELYLRPNSLFLANFMGESSIFEGTIAGNQININGYQFTLANAEQFELPDGECLVG
                     IRPEAIHLAEQGNISQLCKIKNAVYMGNHWEIIAEWEGKDLLVNTKPEN"
BASE COUNT         5841 a         4652 c         4127 g         6127 t
ORIGIN      
        1 attaaataaa ccctaacatt ctataataag aaaaagcggt cgattctctg ccaaattttg
       61 caaattttag aagaaaacag accgcttgtc agggttggaa ttagagtaac cagctgccaa
      121 tcaatgcaca ggcaaaaccg gttaaaccga tggcagtttc aagcattgtc cacgttttga
      181 gggtagtttt ttcgtccatt tcaaggaaac ggctcatcag ccagaaaccg gagtcattga
      241 cgtgggaaag caccgtcgca ccggaggcga tagcaatcac gataaaacag aggtcgaact
      301 ggcttaaatc agtcgctgcc gccacagttg gcgcaatcaa cgctgctgtg gtggttaaag
      361 caacggttgc cgagccttgt gcgacacgga ataccgtagc aataataaag gcggcaacca
      421 caatcggcat tccggtgtct ttcatcattt ccgataatac atcgccaatc ccgctggcac
      481 gcaacacgcc accaaacatt ccgcctgcac cggttactaa gacaatcgag cagattgggc
      541 caagggcatt gttacagatt ttttcgattt ggtcgaaact gcgattatcc cgcaacagca
      601 taatcgccac aattaaggta atcagtaaag caacaggggt tttgccgatt aagcgaaggc
      661 tctgcaccca catttcactg ccatcaatca ctttagcaac ggagagggta ttcaagccgg
      721 tatcaaacaa aatcaggata atcggcagca ccaacaccgt gagtaccttg ccaaaacttg
      781 gcgggttttg tactgccatt tcgtttgccg ataagccgtt taaaaacgct ttcggcaaat
      841 caacgtgaat gcgtttgctg ataaattggc tgaacaggta agtgccgata taccacgttg
      901 ggattgcaca aaccaagccc accaagacta acagccccat attcacacca agcagatcgc
      961 cggaggcaac cgggccgggg tgcggaggta gaaaggcgtg cattaccgca aacgcaccgg
     1021 cagacggaaa cgcatagcgc aacaccgaac cgccgaactg ttttgccacg ctaaaaataa
     1081 tcggtaacat caccaccaag ccggcatcaa agaaaatcgg gaagccgaat aacagcgagg
     1141 caacgcccaa cgcaaaaggc gcacgttctt tgccgaattt attgataagc gtatccgcaa
     1201 gcactttcgc cccgccggtg atttcaagca agcgaccaat catcgcccca agccccacca
     1261 gtaacgccac tgatgccagc gtactaccaa aaccattcaa caatgttggc aggattttat
     1321 ctaccggaat accggcagct aatgcagtga gcaggctcac aataatcaat gccacaaaag
     1381 cgtggacttt aaatttcata atcagcacca gcaataaaat gatggcagca aacataatga
     1441 acagtaacat aggatctcct aaggttgaat tgttatcggt aacataacca aaaagcagtt
     1501 tagagtaaac ggttgcaggg cttttatttt aaaaatgtga tctacttcaa gttttctgga
     1561 aatagcagat gatttttcag cataacctgt tacattaacg gctaagttaa cgttaccagt
     1621 aacacaagga aattcaatgg caaacggcaa agcatttatt ttgatgggcg tgtccagcac
     1681 cggcaaaacc agcgtgggaa cggcagtcgc acagcggttg ggaataaaat tgattgatgg
     1741 ggacgactta caccctcgtg ccaatatact gaaaatggca agtggcacgc cactcaatga
     1801 tgcagatcgc acaccgtggt tggagcggat taacgatgca gtgttcagtt ttgaacaaaa
     1861 aagcgaaaaa gggattatcg tctgctcagc attgaagaaa aagtaccgag accagatccg
     1921 acaaggcaat gccgatgtga cttttctatt cttgcacggc tcgtttgaac tggtgctggc
     1981 tcgaatgcaa caacgcaaag ggcattttat gaaaccggaa atgcttcaaa gccaatttaa
     2041 tacgctggaa attccgcagg cagacgaacc agatgtgata tttattgata ttgacggcag
     2101 ttttgacgag gtggtggagc gttgcgtgaa agcagttgag ccgttgcttt aatcacaagt
     2161 ggtggatttt gcggattttt ttgcaaattt aaccgcttgt ccgggctaca cactttcccc
     2221 caaatggatc tgatagccca agttaattat ggagctttgt tgtggtacgc cgtgaatacg
     2281 gttgagcagc tcttgggcgg caactctgcc tatttcaagg cgaggtgtga tgacgctggc
     2341 aagctgtggc gtgagggatt gcccgacatc gtgtccgtga aagccggcga ttttaatctg
     2401 ctgcggcaca tgaatgccta accgttggca ctcaaataat gccccgattg ccaaatcgtc
     2461 attggtgcaa aaaatgccgt ctagctgcgg aatgtgttgc agggcttgtt ttagctggct
     2521 tgcgccaagc gtgaaagaag aatgctctgc agtagtaatg ctgtgtgggc ttaagccgtg
     2581 tttgagcatt gcctgttcgt agccctgcat tttcagctga gagcgtttat ccattctcgc
     2641 cccgaaatac accacctgtc ggcagcctcg atgaatcatt gtctcgacca ttgcttgagc
     2701 cgcagaaata ttgtcaaatc cgaccgcttg ttgaatgcca cattcgccgc tttccattat
     2761 ctcaatcacc ggaatattcg ccacttccag cattttcaag gtgcggggcg agtggtgatt
     2821 ttccgataag ataatgccgt caatattgta cgaaagcagc gactcaatcc gctgctcttc
     2881 cttttgttcg ctgtaaccat agtgggcgag catggtttga tagctggctt ggtcggtaat
     2941 ctgctcaatg cctttaatca catcggcaaa cacttgattg gtaagcgaag gaaccagcac
     3001 gccaattgca cggcttttgg cattcgagag aatatccggt gcacggtttg ggatatagcc
     3061 aaacagctca atcgcttgtg caatccgctc gccggtctcc ttggcaacgg tgtttggatc
     3121 ccgcaaaaag cgactgaccg tcattttggt aatgccgaga tgggtagcga tgtcttgcaa
     3181 agttgggcgt ttcggttttc tcattggaat agataacgaa aaaatttaat ttcgctgatt
     3241 ataactgaag ataaggcaat aattgcaaaa tattcggtga aattgaccgc ttgttactac
     3301 tcttgcctga actctttcgg ggttttacca aactcttttt taaacacggc atagaaatat
     3361 tgtaaagagg gataaccgca cacattggca atttcttggg tcgaaatctc ggtaaatttg
     3421 agcatatatg ttgctcttgc taatttttcc tcgtgaatca cctggtgaat tgtcttattc
     3481 atttcctgct taaaacgctg ttcaaggtta gagcgggaaa ttttaagatg atccagtact
     3541 tgctctactt taatgccttg gcaagctcgg tgacgaataa agtgcatagc ttggattacc
     3601 attgggtctt gcaaagaacg ataatcggtc gaacggcgtt cttccacttt taccggcggg
     3661 attaaaatcg gcttggttga aaccggtaga ttatttaatt ttcgatataa taacttcgca
     3721 gcgtgatagc cgatttgtga tgttccttgt gtgactgaag aaagagaaac ccgagagagg
     3781 tattgaatca actcttcgtt atcaatgccg atcacgcaaa gttgttcagg tacagcgatt
     3841 tttaaatgct cacaggtttg aagcaggtgt cttgcccttg cgtcagtaac ggcaataatg
     3901 cccgtgtggg ctggtaaggt ttgtaaccac tcgcctaatt tggcttgtaa ttcaacccaa
     3961 ttatctgaat gaaattgatc accaatataa caatttgggt gatgttcata tttttccatc
     4021 aactgaataa aggcattttt tcgctctatc gcccaatgtt tttccgtttc tgcaggatag
     4081 ccgtaaaacg caaattggtt gattcctttt tgttttaggt gtaaaaatgc tgcctcaact
     4141 aaggcgtaat tgtcggtagc aacataagga aggtcagggt aatagtcagg gttttggtaa
     4201 gaaccgccta ctgctacaat ggggacgtca atatctttaa gtagctcaac tgtttccaaa
     4261 tcgtcaaaat cggcaataat gccgtcaatc gacaggcttt cgatactatt tttatgatag
     4321 acaaaatcat cttccacaaa aatatcccaa gtacattgtg atgcttgaat aaactgccca
     4381 atgccctgca ttactcctct gtcataaatt ttgttagcat taaaaagtaa ggcaatttta
     4441 taagattgtt ctatcattac ttcctccagt acccactttc aaaagtaact ataaaataaa
     4501 attatttttt gatcaaaacg aataaaagaa taaaaccccg gtatttctac cagggtttta
     4561 agctcaattt tgattaagat tattccgcag ctgcttcagt tgcaacttct gggcgatcca
     4621 ctaactcaat gtaagccatt ggagcattat cacctgcacg gaaaccacat tttaaaatgc
     4681 gagtgtaacc acccgcacgt tgtgcaaaac gtggacctaa ttcattgaat aatttagcaa
     4741 ctgtatcaac gttgcgtgta cgggcaaaag ctaaacggcg atttgcaacg ctgtctgctt
     4801 tagctaaagt aattaacggc tcaactacac ggcgtaactc tttcgctttt ggtaaagttg
     4861 tcttgatgat ttcgtgttca acaagcgcac ttgccatatt acggaacatc gcttggcgat
     4921 ggctgctgtt acggtttaat tgacgtccac tcttacgatg gcgcataacc tattccttct
     4981 tagaaaatct ataccgaaaa actagtcttc tgcaatactt gcaggtggcc agttttcaag
     5041 gcgcataccg agtgataaac cacgagaagc aagaacatcc ttaatctctg taagagattt
     5101 tttacccaag ttcggggttt taagaagttc aacttctgta cgttgtacta aatcaccgat
     5161 atagtgaatt gtctctgctt tcaaacagtt agcagaacga actgtcagct ctaggtcatc
     5221 tactggacga agaagaatag gatcaaattc cggtttttct tctttcactt caggttgacg
     5281 aacatcacgc aaatcaacga atgcagcaag ttgttctgct aaaatcgttg cagcacgacg
     5341 gatagcttct tcaggatcta acgttccgtt agtttccatc tcaataacaa gtttatctaa
     5401 gtctgtacgt tgttcaacac gagcagcttc gacattgtaa gcaatgcgat ctacaggtga
     5461 aaaacgtgca tctactaata aacgaccgat agggcggtca tcgttttgag tatgaagacg
     5521 tgctgatgca ggaacataac ctcgaccacg ttgaacacga atgcgcatat taattgatgc
     5581 attcttatct gttaaatgac agattacgtg atcaggattc acaatttcta catcgccatc
     5641 gtgtgtgatg tcggctgcag ttacagggcc aattccagat ttatttagtg tcaaaataat
     5701 atcatcttta ttttgcactt ttaccgctag acctttaagg tttaataata cctcaagaat
     5761 atcttcctga acgccttctt tgctgctgta ctcatgcagt acaccatcaa tttcaacttc
     5821 agtaactgca caacccggca tagatgaaag taaaatgcgg cgaagtgcgt tacctaatgt
     5881 atggccaaaa ccacgctcta acggctctaa tgtcacttta gcatgagttg aactaacttg
     5941 ttcaatattt actaagtgcg gctttaaaaa ttctgtcaca gaaccctgca ttttttcctc
     6001 tctttgcggt taagctaact attatttaga gtaaagctca acgatcagat gttcattaat
     6061 atctgctgat agatcagaac gctctggacg acgtttaaat acgccttcca ttttagttgc
     6121 atcaacttct aaccaagttg gtttttcacg ttgagttgct aattctaatg atgctttaat
     6181 acgtgcttgt tttttcgatt tttcacgaac agcaacaaca tcatcaacag aaacttggaa
     6241 tgaaggaatg tttactacac gaccgtttac tacgattgat ttatggctaa caagctgacg
     6301 tgcttctgca cgtgttgctg caaaacccat acgataaact acgttatcta aacgaccttc
     6361 caataatact agtaagtttt caccggtatt accttttaag cggttcgctt ctgtataata
     6421 attacggaat tgacgctcta agataccata aatacggcga actttttgtt tttcacgtaa
     6481 ttgactacca tagtcagata aacgtggctt gcgagcacca tgttgaccag gcgctacatc
     6541 aagtctattt ctacattttg attcaatcgc acgaacgcct gattttaaga ataaatcagt
     6601 accttcacga cggcttagct tgagcttagg acccaaatat cttgccattt tctttctcca
     6661 attcttttaa cgtcaattaa acacgacgtt ttttcggtgg acgacaaccg ttatgtggaa
     6721 tcggagtcac atcagtaata ttcgtgatac ggaaacctgc tgcatttaat gcacggattg
     6781 ttgactcacg acctggaccc ggtcctttaa ccataacttc caaattcttt aaaccaaact
     6841 ctttaaccat ttctgcacaa cgctctgcag caacttgagc agcgaatgga gtcgatttac
     6901 gagaaccacg gaaacctgaa ccacctgcag ttgcccaagc tagagcatta ccttgacggt
     6961 cagtaatagt aacgattgtg ttattgaaag atgcgtggat atgagctacg ccatctgcaa
     7021 tctgtttttt tacgcgctta cgtgcgcgaa ctggtgtttt agccattatt tatctactcc
     7081 gactattttt tgatcggttt gcgtggacct ttacgggtac gcgcattagt cttagtacgt
     7141 tgaccacgta ccggtaaact acgacgatga cgtaaaccac ggtagcaacc taagtctaaa
     7201 agacgtttga tgcttaaagt tacttcacga cgtaaatccc cttcgacagt aaatttacca
     7261 acttcttcac gcagtttttc aatctgctct tcagacaatt ctctgatctt tacatcttca
     7321 gcaatacccg ttgcagcaca gatggctttt gcacgagttt taccgatacc gtaaattgca
     7381 gttaatgcga ttacagtgtg tttttgatca ggaatgttaa tgcctgcaat acgggccact
     7441 atgcactcct attatttaag tttattgata tactgatctt gaaaagcccg tttcaggata
     7501 ctcaaacagt atatcaggac gaatgagctg agcagtataa ctgctcagcg ggttctttgc
     7561 aagaaatatg cgtaaattaa ccttgacgtt gtttgtgttt aggatcgcta caaattacac
     7621 gaaccacacc ttcacgtttg attactttac aattacggca cattcttttt actgaagcac
     7681 gaactttcat tgcttatcct ttattaacgg cctattaatt tagacctttt aagttagctt
     7741 tcttcaacac agaatcatat tgttgagaca ttaaatgact ctgaacctgt gcgatgaaat
     7801 ccataataac aaccactaca atcaataatg atgtaccacc taattggaat ggcaccttcc
     7861 ataacgacgt aatgatgtaa ggaaccaaac aaacaaaagc aatatataac gcaccaatta
     7921 aggttaaacg tgtcattact ttatcgatat aacgagatgt ttgttcgcct ggtcggtaac
     7981 ctggtacaaa cgcacctgat ttcttaagat tatccgctgt atcacgagga ttatactgca
     8041 ttcctgtata aaagaaagca aagaaaatca ctgccattgt gaataataca acatacagtg
     8101 gttgacctgg ctgtaatagt tgtgaaaggt taaataacca ctcaaatcct tcgctttgac
     8161 caaaccattg agtaatgctt gccgggaata aaataatact tgaagcaaaa attgccggta
     8221 ttacccctgc catattcact tttaatggta agtgagaagt tcttgcaggc gccatcattc
     8281 gaccttgttg gcgacttgca tagtttacaa ctattcttct ttgtccacgt tcaacaaaaa
     8341 caacaaaata agttactgca aatgcaatta ttgcaactag tagtaagacg ataaatgata
     8401 actcgccttg acgggcttgt tctattgtct gccctatcgt tgctggaaga tctgcaacaa
     8461 tacctgtaaa aataatcaat gagataccgt ttccgatacc acgctcagtg atttgttcgc
     8521 ctagccacat taggaacatt gttcctgtca ctaagcttac tacagaggtt acatagaata
     8581 atatacttgg attaggaact aatccatgta ccatattcgg tagggctagt gaaataccaa
     8641 tagattgaat aaatgctaat aacagtgtgc catagcgtgt atatttactg attttccgac
     8701 gacctgcttc accttctttc tttaattcag ctagaggtgg gtgaatcgct gtcaataatt
     8761 ggataataat tgacgctgaa atataaggca taatacctag tgcaaatata gatgcacggc
     8821 ttaaagcacc accagagaac atattaaaca tatcaatgat ggtgccttgc tgttgtcgaa
     8881 ttaattcgga taatacagaa gcatcaatgc caggaacagg tataaaagaa ccgatacgga
     8941 aaacgattaa cgcacctaaa acaaacaata gtcttgattt aagctcgcca gtcccttttt
     9001 gtgtactacc ttggtaccct ggttgctttg ccatctttat tcctcgatag aaccgccagc
     9061 agcttcaata gcagctttag cgcctttagt tacacgtaag cctttaacaa ctactgctct
     9121 ttcaattttg cctgctaaaa ttactttagc agataaaata tctttagtaa tgatgtttgc
     9181 tgcttttaat gtctctaaag ttacttcgtt gccatctact ttagctaagt cgtttaaacg
     9241 aatttcagcc acatggaatg attttaatga agtaaaacca aattttggca aacgacgata
     9301 taaaggcatt tgaccaccct cgaaaccacg acgaacaccg ccgcctgtac gagatttttg
     9361 acctttatga ccgcgcccac cagttttacc taaaccagaa ccaataccac gacctagacg
     9421 ttttgcactg tgcttagcac cttcagctgg agaaagagaa tttaaacgca ttctattact
     9481 cctctacttt aaccatataa gacacttgat tgatcatacc acgcactgct ggtgtatctt
     9541 caagttctac agtatgacga atatgacgaa gacctaagcc ttttaacgtt gctttatgtt
     9601 tcggtaaacg agcgatagag ctacgagttt gagttacttt aatttttgcc atatccaatt
     9661 accccaaaat ttcttcaact gttttgccac gttttgcagc aaccatttca ggtgatttca
     9721 tgttctcaag tgcatcgatt gttgcacgaa caacgttaat tgggttagtt gaaccatacg
     9781 ctttagaaag aacgttacga acacccgcaa cttctaatac tgcacgcatt gcaccaccag
     9841 cgatgatacc tgtaccttca cttgctggtt gcataaatac gcgtgaacct gtatgtgaac
     9901 ctttaactgg atgttgtaat gtaccttcat ttaaagctac attaatcata ttgcgacgag
     9961 ctttttccat cgctttttgg attgctgctg gaacttcacg tgctttaccg tgaccaaaac
    10021 ctacacgacc gttaccatcg cccactactg ttaaagcagt gaaactcata atacgaccac
    10081 cttttacggt ttttgaaaca cggttaaccg cgattagctt ttcctgcagt tcaccagctt
    10141 gtttttcgat gtttgacatc tcaaattcct ctattagaac tgtagaccag cttcacgagc
    10201 agcatctgct aatgattgca cacgaccgtg gtatttaaaa ccagaacggt caaaagcaac
    10261 cgctttaata cctttggcaa ttgcacgctc agcaacgatt ttacccacta ctgctgcggc
    10321 ttctttattg ccggtatatt taacttgctc tttaattact ttttcaacag ttgaagctgc
    10381 tgctagtact tctgagccat taggtgcaat cacctgcgca tagatatggc gaggtgtgcg
    10441 atgcacaact aaacgtgttg caccttgctc tctcattaaa tgacgtgcac ggcttgcacg
    10501 acggatacga gctactttct tatccatagt gttaccttac tttacttttt cttcgcttct
    10561 tttgtacgca ctacttcatc agagtaacgt acacctttac ctttataagg ttcaggtttg
    10621 cgatatgcac gaatatctgc tgccacttga ccaattaact gtttgtcagc acttttcaga
    10681 ataatttctg tttgtgatgg acattcacca gttacgcctg ctggcaacgt gtgttctact
    10741 gggtgagaga aacctaaact taaagcaaca gcattgcctt tcatttgtgc tctataacca
    10801 acacccacta attgcaattt cttagtaaag ccttcagtaa ccccgattac catattattt
    10861 actagtgcac gagcagtacc tgcttgagca tctgcattag cgataccttc acgtggagcg
    10921 aaagttaatg catctgcttc ttgttttact tcaactgcat tgtgaatttc acgagataac
    10981 tcgccgtttt tacctttaac tgttaatagc tgaccgttaa gttttacctc aacaccggca
    11041 ggaatattaa caggtgcttt tgcaacacga gacatttttc ctacctctct attatgctac
    11101 gtaacagata atttcaccgc cgaggcccgc ttgacgcgca gcacggtcgg tcattacacc
    11161 tttagatgta gaaacaactg cgatacctaa accacccatt acttttggta attcgtcttt
    11221 gcgtttataa atacgcagac ccggacggct cacacgttgg atactttcta caaccggttt
    11281 gttttggaaa tattttaaag taatttccag ttcaggttta acaccttcaa caactttaac
    11341 gctttcgata taaccttctt cagctaaaac atttgcaata gcaactttta acttagatga
    11401 aggcatactg attgcaactt tgttcgcagc ttgaccgtta cgaatacggg tcagcatatc
    11461 tgcgattgga tcttgcatgc tcatgtattt actcccatga ttccaaatta aaagaggtta
    11521 ttaccagctt gccttcttaa gacccggaat ttcaccgcgc atagcagctt cacgaacctt
    11581 aatacggctt aaaccaaact tacgaagtac gccatgtgga cgaccagttt ggcggcaacg
    11641 attacgctga cggattgggc tagaatcacg aggaagtgtt tgtaatttta acactgctgc
    11701 ccaacggtct tcatcagatg cattcacatc agagatgatt ttctttaatt cttcacgttt
    11761 tgcgtagaat ttatcagcta atttagcacg ttttacatcg cgtgcgatca ttgattgttt
    11821 tgccacgata acctacctta tttacggaat gggaaattga aagcagctaa taaagcttga
    11881 ccttcttcat cagttttcgc tgaggtggtg atagtaatat ctaaaccacg aacacgatct
    11941 actttatcgt agtcgatttc agggaaaatg atttgttcac gaacacccat actgtaatta
    12001 ccacgaccat cgaatgactt agcgtttaaa ccgcggaagt cacgaatacg tggaacagcg
    12061 atagtaatca atctttcaaa gaattcccac atacgttcac cacgtagggt tactttgcaa
    12121 ccgattggat acccttgacg gattttaaag cctgcaacag atttgcgagc tttagttact
    12181 aaaggttttt gaccgctgat tgctgctaaa tccgctactg cgttatctaa cagttttttg
    12241 tcggtcaatg cttcacccac acccatgtta agggtaatct tttcgattcg tgggacttgc
    12301 atgacagatg agtagttgaa ttttgctttt aattcattaa ctactgtatc tctgtagtaa
    12361 tcatgcagtt tcgccatcac attactccag ttaattaaat tgttttctcg ttagatttga
    12421 agaaacgcac ttttttgcca tcttcgaatc taaatcctac acggtcagct ttgttggttt
    12481 cagggttgaa gatcgcaacg tttgaaacat cgattgcagc ttctttcttc actaaaccac
    12541 cagctttgcc taatgcagga actggttttt cgtgtttagt gatgatgttg ataccctcaa
    12601 caaaaacttt accatttggt aacactttag ttaccttacc acgtttgccc ttatctttac
    12661 cggtaagaac aattacttca tcgttttgac ggatttttgc agccattaca ttctccttat
    12721 agtacttctg gagctaaaga gatgattttc ataaacttct cagaacgaag ttcacgagtc
    12781 accggtccaa aaatacgagt accgattggt tgctcagtgt tattgtttaa aattacacaa
    12841 gcattgccat cgaaacgaat aactgcgcca tctgggcgac gaacaccctt cttggtgcgc
    12901 acaacaactg cttttaacac atcacctttt ttcactttac cgcgtggaat tgcttctttt
    12961 acagtaactt taatgatatc gccaatagca gcgtaacgac ggtgcgatcc acctagaacc
    13021 ttgatacaca ttacgctacg agcccctgag ttatcagcaa catccagcat agtctgttct
    13081 tggatcatat tagtgctccg ttaaaataaa aaaacaccct ttcgggacga acctatttat
    13141 acaaatataa atagggtgca aaatatacta caaaaaccac tagttaatca agtgaatttt
    13201 taagctaatt taatagttta agtaagctta tttataaatt gtttcaacta tcaataaact
    13261 gtcctataac tcatataaaa accccaagat atcttggggt ttaactaaca ttactctaca
    13321 gtcaccgctt ttgcaaggtt acgaggttgg tcaacgtccg ttcctttgat taaggcaatg
    13381 tgatatgaaa gtagttgtaa cggcacggtg tagaaaatcg gtgcggttac ttcatcaact
    13441 ttcggcatta caatcgtttt gaagccttct gcttcttcaa aacctgcatc gtgatcggca
    13501 aacacatata actgaccgcc tcttgcacgc acttcttcaa tattggattt cactttctca
    13561 agcaagtcat tttccggtgc taccaccact accggcatat cgctatcaat taatgctaac
    13621 gggccgtgtt ttaactcgcc tgcggcataa gcttctgcgt gaatatatga aatctctttt
    13681 aatttcaatg cagattccat cgcaaccggg taatattctc cacgacctaa gaacaatgtg
    13741 tggtgtttat ccgcaaaatc ttccgataat ttttcgattt gtttatcgaa cactaacgca
    13801 ctttcaattt gtgctggtaa gcgttgtaaa gattgcacga tatggtgttc ttgctcttca
    13861 ctcattctgc cttgtaagcg tccaatcgcc acatttaata acaataagca agttaattga
    13921 gtagtgaatg ctttggttga tgctacacca atttcaacac cagctttggt taagaatgca
    13981 aaatcggatt cacgcactaa tgaagaactt gccacattac acaccgtcat tgccgccata
    14041 tagccagatt ctttcgctaa acgcagtgca gctaacgtat ctgcggtttc acctgattgt
    14101 gaaagcgtga ttaataagct gttcgggcga gtaacgaatt tacggtaacg gaattcggag
    14161 gcaatttcca catcacagct cacgcctgca atggattcaa accaataacg tgaaaccata
    14221 ccagcattgt aagaggttcc acaagccaca atttgcacgt gttctacttt cgataaaatc
    14281 tctgccgcat tcggtgcaat ggctgaaata tctactttac cgtctttgat acgaccatct
    14341 aaggtattca taatcgcaac cggctgctca aaaatctctt tttgcatata gtgacgatat
    14401 tgtcctttat ctgccgcgtc tgcctcaaaa ttgccttcgt gaatttcacg ctctgccttg
    14461 ttgccttcac ggtcataaat atccactgtg cgacgggtaa tttcggctac atcaccttct
    14521 tctagataag caaaacggcg agttacgctt aataatgcaa gtgggtctga agcaaggaag
    14581 ttctcgccta caccgtaacc aatcactaac gggctacctg agcgagccac aattaaatgc
    14641 tccggctctt cttcatttaa taccactgtg ccgtaagcac cacgcagttg caccacggtt
    14701 ttttgtaccg cttccaataa tgaagaagca gaacgcaatt cccattcaac taaatgagca
    14761 atgacttcgg tatcggtttg agattggaac acataaccac gctcttgtaa cactattttt
    14821 aactcttcgt agttttcgat aatcccgtta tgaactactg caattttgcc actgcggtgt
    14881 gggtgagcat tagtttctga cggctcgccg tgcgttgccc agcgagtgtg tgcgatccca
    14941 gtgccgccta atagtggctt agtgtctaac gcctcatcta aggctttgac tttaccgaca
    15001 cggcgtacaa tttgcattgt tttatcatct gcatttaata ctgctacacc agcagagtca
    15061 tatccacggt attctaagcg atgtaaacca tcaaccaaga tttctgcgat atcacgttgt
    15121 gctactgcac ctacaattcc acacataaat ttgctttcct atgtttaagt ttaaaaattc
    15181 atttgagcta ctgctatttt gcaataacca cttcgacacc cattttttcg atttgttctt
    15241 tcgtttcaaa acttaattga ctgtcggtaa taattttggt aatattttgc catttcaatt
    15301 caatattcgg cattttccga ccgatttttt gcgattcaat taacacaatc acttcacggc
    15361 tcacttctgc catcacctga cttaagccaa ctaattcatt aaaggtagtc gtgcctcgag
    15421 ccaaatctaa accgtccgca ccaataaaaa gctgatcgaa atcatatgaa cgcaatacct
    15481 gttctgctac tttgccttga aaggactcag aacggctatc ccaagtgccg ccggtcatca
    15541 acactgttgg ttcgcattcg agggaagtca attccgtcgc taaattcagc gaatttgtca
    15601 tcaccaccaa gccggtttgg ttcaatgctt ttaccaaagc tccggtcgtg ctgccgctgt
    15661 caataatgat gcgattatga tcacgaatac aatcacgtgc ggcttgagca atcattttct
    15721 tttgaatcga aagttgctca ataaacgctt cttccatcat ttccgatgga atttttaccg
    15781 ccccaccata cttacgcaat aaaaaaccac tttcttccaa agcggttaaa tcttttctaa
    15841 tcgtaacttc agacgtctca aagaaatgag cgagttcatc tacactcact tcatttttct
    15901 cttgtaccat ttgaacaatt aaatggcggc gttgttgcgt gttacgtttt gacattataa
    15961 tcttcactta aatttcgttt caaaactttt gcattataaa gaaagctcgc gaaaagaaaa
    16021 gcagaaaatg caaaatttac aaaatctata caaaataaaa ccgcttgtaa tattgattta
    16081 caagcggttg atttcttgaa attttttgca attatttgat ttggctacca atcacgccac
    16141 caagtgcggc accacctaaa gttgcaccgg tagattcacc aatcgcgtga ccagccacac
    16201 cgccaattgc tgcaccgatt gcagtattac gttgcgtttt tgtcatattg ttacagccag
    16261 acacagcaac cattaatacc attgcaccaa caactttcaa aatagatttc ataataatcc
    16321 ctcataatta atcaatttca aataagccaa tattcttggc tatgctataa gatttaattt
    16381 atttagaaag gttcaactac taccacctat gaattgcaaa ttttttcaca atttaggccg
    16441 cttgttgatt gaactgtgag cttgctcaca gaattttatg gtatttcttg ctatattgta
    16501 tagcaatcta tgcgataact cattaaggaa ataaaaatga aattaaaaaa attatcttta
    16561 gcactctcga ctttattact cggttcagcc tttgccaact ctgctttagc ggaaggtcgt
    16621 ttaaccgttt attgtagtgc cactaatgaa ctttgtgagc aagaagtgca agcattcggc
    16681 aaaaaatata atgttaaagt agcatttgtg cgtaatggtt caggcagtac attggcgaaa
    16741 attgaagcgg agaaaaacaa tcctcaagct gatgtatggt atggcggtac tttagatcca
    16801 cattcacaag ctgcagaaat gggtttactc gagccgtata aatcaccgaa tctcgaacaa
    16861 attatcgaaa aattccgtga gccggcaaaa ttaaaaggca actactcttc ggctgtgtat
    16921 atggggattt taggttatgg tgtgaattta gaccgaatca aaaaactcgg cattgaaaaa
    16981 gtgcctagca cttgggaaga cttgctcgac ccacgcctag ccggtgaaat ccaaattgcc
    17041 gacccacaaa gctccggcac ggcttatact gcgattgcga ccttcgtaca acttttagga
    17101 gaagaaaaag ccttcgacta tttccgcaaa ttacacaaaa acatttcaca atataccaaa
    17161 tccggcatca caccggcacg caataccgcc cgtggtgaaa ccgctatcgg tatcggtttc
    17221 ttacacgact atgcgattga gaagaaaaac ggggctaaca ttgaaatgac cgcaccaaca
    17281 gacggcacag gctacgaatt aggtggtgtg agtatcctca aaggtgcaag aaatcttgat
    17341 aatgcaaaac tgttcgtgga ttggtcgctt tctaaagaag ctcaagaact ctcttggcaa
    17401 aaaggcaatg cgttccaagt attaaccaac accaccgcag aatcttcccc ttatgctcta
    17461 gatccgaaaa acttaaactt aatcaactat gatttcgaga aatacggctc gagcgaagag
    17521 cgtaaacgct tgattgataa gtgggtaaat gaagtaaaat tagcgaaata gtgttaatct
    17581 ttccactgag gggataagca attatcccct ctctaattat aagaatctgt gaggtgttat
    17641 gcagaatttt tccttttccc gaccgcttgt ttgggtgctt atcgggttac tcggttttct
    17701 cttgttaccg tctaaagcct tagattatgg gctgtttgac tcaacctctg atgaactgct
    17761 cgccgcaatg ggctgggcga gtctgaacat cacttgggcg tggtttttac cgcttttggt
    17821 atttttattg ccaaattcga ccgcttgtaa aactagagcc aagctggaat tagtagggct
    17881 gggcgtgctg ttcgccttta ttttcctgtc agcgattgtg ctgaaaatca gcctcggtta
    17941 tgcggtgttg ccgttgatta ttgccattat ggggcttgcg accccaaatt tagcaacgct
    18001 caaagtaatg cagggtgacc gctttattat cggctcgtta ctgagcatta ttctgctgat
    18061 tttctttttt attgtgtatc cgaccatcgc catttttgtg tcgatgttct acaacggctc
    18121 ggaatttgtg ccaagccaag tgctgacaat tttgcaacaa ccttatgtcg ttcgagtcat
    18181 cggtaactcg ctctcggttg ccggcacggt gggcgttctc tccaccctat tcggcttagc
    18241 ctttgctctc tacaccactc ggattgctaa acgctcggcg tttatcgggc ggattttctc
    18301 gattttacct atcgtcaccc cgccgtttgt ggtcggttta ggcgtaaccc tgatgcttgg
    18361 gcgttccggc tatgttactg aatttttagt ggattacttt ggctttgcca acaattggct
    18421 gtacggtttc accggtattg tgatagccca taccctcgcg ctcacgccaa tgtcgtttat
    18481 gattttggaa ggggcattga aatccattca cccatcggtg gaagaagcag cttacaccct
    18541 gcgttccaac cgctatcaag ccttttttaa cattattttc ccattgttaa aaccggcatt
    18601 ggcaaactcc tttttagtgg tggcaattca atccctcgcc gatttcagta cgccgttagt
    18661 attgggcggt agctttgatg tgattgcaac ccaaatctac ttctacattg ccggttctca
    18721 actggattat gcctctgcta gcacgcttgg ggcaattttg ttggtgttct ccttagccat
    18781 tttcgtgatc caatacctct ggatcggcaa tcgctcttat gtgacggtgt ccggcaaatc
    18841 ctatcggggt gatgtccaag acttaccaag cggtatgaaa tggctgatta tttgcacctt
    18901 attgttctgg ggaaccttca acttagtgct gtacggcagt attttctacg gcagtttcac
    18961 cgtgaactgg ggtgtggatt ataccttaac ccttgataac tacatcaagc tattcgggca
    19021 agggtttagc gatggcggtt tcccttcgtt aattcaaacc gtgattttcg ctgccgctgc
    19081 cgccccgatt actgcactat tcggcttgct gatcgcctat gtgacagttc ggcgtgattt
    19141 caaaggcaag aaaaccctgg aatttttaac cctgctctgc tttgccgtac cgggaacggt
    19201 ggcaggtgtt tcttacattc tcgcctttaa cgatgccccg atttacctaa ccggcaccgg
    19261 tatgatcatc attctctcaa tggtaatgcg gaatatgcct gtgggtatgc gagcggcaat
    19321 tgccggctta gggcagttgg ataaatcgct ggacgaagcc tcgctctcgc tgaaagccgg
    19381 ttcgttcaaa acgatgctgt ttgtggtgtt accgctactc aaaccggcac tgctctccgc
    19441 cctcgtcacc agctttgtgc gggcaatgac gactgtcagt gcgattgtat tcttagtcac
    19501 cgccgacact cgtgtggcaa cctcctacat tctcaaccgt gtggaagacg gtgaatacgg
    19561 cattgcgatt gcctacggct caattttaat tgtggtgatg atggcgatta tcctgatttt
    19621 tgactggatt gtcggcgaca cccgtatcgc ccgttctaag gcgaaaaaaa tgtaaaaaga
    19681 catctactct ctccctttat gggagagaga cagaaaaata cttcgttcag aagtattttt
    19741 cagagagagg gatataatta aaaggacaat atatgaacaa cgatttctta gtattaaaaa
    19801 acgtcaccaa atctttcggc aaggtgacgg tcattgataa tctcaacctc agtattccgc
    19861 aaggtaaaat ggtgacatta ctcgggccgt ccggctgtgg taaaaccacc gtactgcgtt
    19921 tagtggcagg gctggaaaac ccgacttcgg ggcaaatttt tattgatggc gaagatgtta
    19981 cccaaagttc gatccaacac cgtgatattt gcattgtgtt ccaatcttat gcgttatttc
    20041 cgcatatgtc gattggcgat aatgtcggct acggcttgaa aatgcagggc gtaccgaaag
    20101 aagaacggct gcaacgagtg aaagaggcgt tggaattggt ggatttaggc ggctttgaag
    20161 atcgctttgt cgatcaaatc tctggcggac aacaacaacg tgtcgccctc gcccgtgcct
    20221 taattctgaa accgaaagtg ctactgttcg atgaaccgct gagtaacctt gacgctaact
    20281 tacgccgttc aatgcgggag aaaatccgtg aattacagca acgtttaaac attacttccc
    20341 tttatgtgac tcacgatcag tccgaagcct ttgcggtttc agatgaagta attgtgatga
    20401 acaagggaaa aattatgcaa aaagctccag ccaaagagtt ataccttcgc cctaattctt
    20461 tattcttagc aaattttatg ggtgaaagca gcatttttga aggcacgata gctggcaacc
    20521 aaattaatat aaatggatat caatttacgt tagcaaatgc tgaacaattt gagttacccg
    20581 atggggaatg cttagttggc attcgtccag aagctattca tttagctgaa caaggaaata
    20641 taagtcaact ctgtaaaatt aaaaatgcag tttatatggg aaatcattgg gaaattatag
    20701 ctgaatggga agggaaagat ttattagtca atactaaacc tgaaaat
//
LOCUS       DANLYI010000018        20723 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.018, whole genome shotgun sequence.
ACCESSION   DANLYI010000018 DANLYI010000000
VERSION     DANLYI010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 20723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20723
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.018"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            53..1498
                     /locus_tag="PXD81_000533"
     CDS             53..1498
                     /locus_tag="PXD81_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017806747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HDL5536597.1"
                     /translation="MAAIGSAVGLGNIWRFPYTTYENGGGAFILPYIIALLTAGLPLL
                     FLDYSIGHKYRGGAPLAFRRFSSKFETFGWWQVLINVIIGIYYAVVLGWAASYTYFSF
                     SGAWGDKPVDFFIGEFLKMGDITQGVSFEFVGMVTGPLIAVWLIAIAVLAMGIKKGIS
                     AASNLLMPQLIVMFLVLVGYSLTLPGAAKGLDALFTPNWEKLADPSVWIAAYGQIFFS
                     LSICFGIMITYASYLKKETDLTGSGLVVGFANSGFELLAGIGVFAALGFMATAAGQEV
                     NEVAKGGIGLAFFAFPTIINQAPMGALVGVLFFGSLTFAALTSFISVIEVIISAVQDK
                     LNSGRVKATLLVGIPMMVVSVLLFGTTTGLPMLDVMDKFVNQFGIVAVAFVSLIAIVT
                     SEKLSTLEKHLNEVSSFKVGFIWRLCIVITSGVLAFMLFSEIAKVYREGYEGYPSWFV
                     NTFGWGMAIGLVVVAYLLSKMAWKNEPKGDE"
     gene            1498..1596
                     /locus_tag="PXD81_000534"
     CDS             1498..1596
                     /locus_tag="PXD81_000534"
                     /inference="COORDINATES: protein motif:HMM:NF028261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine/alanine import family NSS transporter
                     small subunit"
                     /protein_id="HDL5536598.1"
                     /translation="MSSAAIITMVVAMTIIWGGLLFAIKRLPKEEQ"
     gene            complement(1644..1907)
                     /locus_tag="PXD81_000535"
     CDS             complement(1644..1907)
                     /locus_tag="PXD81_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018650935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrxA family glutaredoxin"
                     /protein_id="HDL5536599.1"
                     /translation="MFVEIYGRLSCPYCVRAKTLAEKMKQELSDFDFKFIDMIAENIS
                     KQDLEPRVGKPVATVPQIFVDNNHVGGCTDFQAFVEEKFGITV"
     gene            2067..3401
                     /gene="srmB"
                     /locus_tag="PXD81_000536"
     CDS             2067..3401
                     /gene="srmB"
                     /locus_tag="PXD81_000536"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HDL5536600.1"
                     /translation="MTQLMTFEELDLSPQLLKALAHKGYNRPTAIQQATIPAALEGRD
                     LLGSAPTGTGKTAAFLLPAIQHLLDYPRRKPGAPRILILTPTRELAMQVAEQAQALAK
                     FTQLSIATITGGVAYQNHGEVFNKNQDIVVATPGRLMQYIKEENFDCRAVEVLIFDEA
                     DRMLQMGFGQDAEKIAAETRWRKHTWLFSATLEGELLEDFAERLLNDPIKVDAEPSRR
                     ERKKIQQWYYHADNVEHKTKLLARIISEFSVERAIVFVRRREDVRELSETLRKRGIRS
                     TYLEGEMAQTQRNQAINRLKEGVVNVLVATDVAARGIDIDDIDFVLNYDLPYSADTYL
                     HRIGRTARAGKKGSAISLVEAHDYKLLGKIKRYTEEVLKPRIIEGLEPRTKAPKDGEL
                     NVTSKKEKARIKKKKEAKKEAAKPKVKVRHKDTKNIGKRRKPSSESAAVKQA"
     gene            complement(3470..3763)
                     /locus_tag="PXD81_000537"
     CDS             complement(3470..3763)
                     /locus_tag="PXD81_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005598606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HDL5536601.1"
                     /translation="MSKTMQQKAAEAHNMCRLKGDSMLDNADRQIAFEAVYESEELAQ
                     QAVEFFAQKAKSVETEPCEIQSEITQIAEGFLMKMWITFSCQAEVILFQMAVR"
     gene            complement(3765..4427)
                     /locus_tag="PXD81_000538"
     CDS             complement(3765..4427)
                     /locus_tag="PXD81_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HDL5536602.1"
                     /translation="MPFPDEISELKGYFDGTNPVSAYPAQGLNVPFYILGSSPESAYL
                     AAERGLPYAFASHFAPRFMDDAIRIYRSHFKPSDVLDKPYVILGVNAIVAETDEEATQ
                     LATTQTQFFLNVVTNAQQNLQPPLESDDKVWENFVKAEIVPHFGPVAFEQNALYRQEK
                     TVVRQMTECSLIGSPQSVEQQLKALKARVEIDEIMAVNYIFDEQKQHQSYTWLKQITD
                     KV"
     gene            complement(4411..4863)
                     /locus_tag="PXD81_000539"
     CDS             complement(4411..4863)
                     /locus_tag="PXD81_000539"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MsnO8 family LLM class oxidoreductase"
                     /protein_id="HDL5536603.1"
                     /translation="MVLQVLNSKAIQPFLYANSENKMTNLSILNLVPIREGQTAKQAV
                     DSMVKLAKHAENIGIERYWIAEHHNMKNLASSATQLLIQHALSHTEKLRVGSGGVMLP
                     NHSPYIVAEQYGTLETLYPNRVELGLGRAPGTDMRTASALRPEFAFPR"
     gene            complement(4883..5521)
                     /locus_tag="PXD81_000540"
     CDS             complement(4883..5521)
                     /locus_tag="PXD81_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005602749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HDL5536604.1"
                     /translation="MKKFALKTVLVALSALFAFNATAVAADPTAGKEYIEVRKAPSAQ
                     KEVVEFFSFYCPHCYDFELNYKIPSQIKAKLPADSKLVQYHVNFLGRQSENLTRAWAL
                     AMALGAEDKVKTALFEAAQKDAFKSMDDIRAVFIANSISAEQFDSGINSFAVNGLVNK
                     QVQLAEDFQIRGVPAFFVNGQYQLNLEGFSDSSSTNDFIQRYIDAVVFLSKK"
     gene            complement(5565..5834)
                     /locus_tag="PXD81_000541"
     CDS             complement(5565..5834)
                     /locus_tag="PXD81_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HDL5536605.1"
                     /translation="MKCKRLEELLELLGEHWRKNPDLHLIDILQQLSVEVSEPDNFHA
                     LRDEVLIYQLKMRNADKFEPIPGIKKDYEDDFKTALLRARGILND"
     gene            5926..7035
                     /gene="mobA"
                     /locus_tag="PXD81_000542"
     CDS             5926..7035
                     /gene="mobA"
                     /locus_tag="PXD81_000542"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HDL5536606.1"
                     /translation="MEEKISAVILAGGLARRMNGAEKGLQLLDQKPLISHLLARLSPQ
                     IEDIWLNINRSVEQYQQLYPELPYYQDSLPDFQGPLSGMLSGFEQIESDYLLFVPCDT
                     PFIPTQLLHKLHTALRINQAQIAYAHDGDRPHPTVALVHRSVAENLRAYLASGERRLL
                     WFFQTQKSVAVDFSAYKAAFRNFNCLEDLTAFNPYPIKTLAITGYSGTGKTTLLEKLI
                     PKLTACGIRVGLIKHSHHNVDIDKKGKDSYRLREAGANPTMVVCDERWALMTETKQAA
                     ETKQAVEFSQLIAKFDPETVDLVLVEGFKHETMPKIQLHRKEIEKPLPALDQWTIATA
                     TDYPLERENRLDINDVEQLADFIRQWLEASKEPAN"
     gene            complement(7090..11601)
                     /locus_tag="PXD81_000543"
     CDS             complement(7090..11601)
                     /locus_tag="PXD81_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HDL5536607.1"
                     /translation="MINKNFPLSYLASFVAFAVSLSATASVFRDDIDLQYYRDFAENK
                     GKFTVGASNIEIKDKSGNTLDALFPNVPMPDFSAANRNLGIATLVAPQYLVSVAHNTQ
                     YNTVEFGAPGTNADAHHYTYKVVDRNDYGIVEGGQHQDYQVPRLNKLVTEVAPATVTD
                     LGNNASAYQDSSRFTHFARLGSGRQIVKNTEHKDNQISTSYQYLTGGVHLPIAVHNSD
                     YWLDFRGNALNNSPYGALTAFGTRGDSGSGVYGYDQKTKRWLLLATYTFGTPANNYYN
                     RAGIIRQDYHDKQFAEDIAGTLTNAQQNAVFEWSAQGKDSSIGNKGKNLTVSLADRSI
                     KDNSASPLGYGGTPQLQLPQDNTGKTLNIEGQDSTIVLKTDIDQGAGALNFNANATVR
                     PENDQSWVGAGIVVAKGKQVNWQVKNPQGDRLSKLGEGTLHINGKGENLGDISVGQGT
                     VILTQQAGENGKKSAFNQVGIVSGRSTVVLNSADQVDPNKIYFGFRGGRLDLNGNNIA
                     FNRIQNSDDGARIVNNHLQKAATLTINGPKPPEATDLKWGTWKENSADIYEYINPHAN
                     NRTDYFTLKGNPNQYMPTNGASNAHWTFLSSNKDAAVKQVLAQKGLEHRYNSFNGFIG
                     ETDNTQHNGRLNVVYDPKASTPPATASEVTWGKGLVAGADIYLFTNPKTKIREYFALK
                     GDPKQPVPRGGLSSEHWEFLAADRNQAINKVLARKNAPIEQEKLNSIYTFSGGLNLNG
                     DLTVKGGKVLLSGRPTPHAYDAINKQDVVYADDWQNRQFKADNMQLNQYAQLYVGRNV
                     SNLQANLSANDHAQLHLGFINEQTPSCYYSEYTGKTSCDTQAVVSNEIFATLPTTQIN
                     GDVSLAEQSQLHIGKANLIGTIQAAATTSIRLANQASWTNTGDSRTGNLVAENGSTIN
                     LNEKFVTGEIPTRFNTLIIDGNFQGNAKINYLTDIAAGKGDHLQVNGLAEGTFTLALR
                     NSGKEAEVVSPLSLLTLTHQAQADKAKVSLENGYVDLGAYRYVLANRSNGYRLYNPLK
                     DAKDRNQSIATAKAELDHAIAEADKQKQEISRLNAEAEKERQAEKNAKQAAANAQSQL
                     SQANSELTRLQQYADYYRRYYPTYYRQIQGQITVAKQKVTQASAALTTAENNAKASAA
                     QIANVEQAVETAQNIAKQVEAKLATLRITAGNSEAILNAEALKLCQENGANCEHLQAH
                     ADTDSSNIQQSDWVSQYANTALSELSAQANSALQIGQDLDRQLFAKHDKFHVWSSIEH
                     QKTEHQSDLYRPYEQQTNLTQLGIELPLANGINAGVMLSRNHANAEFDEGVNGKSNLL
                     MASLYGKWHSENGTFVSLDGSYGKAKNRIDLFGENRFNRHIMAIGANLGHNFDLAGVQ
                     VQPAVGTRYYRFSAQNYKLGEVEVRSPKAHFMAYQAGVKVSKAFDLSSWKVEPSLAAH
                     YVDASSKRLSVAVNDNTFSQRFGRYLKTEVGVGVTKGQWQLSSHLGLLKGNEIGKQHF
                     AGFKLGYSW"
     gene            complement(11785..12789)
                     /gene="fbp"
                     /locus_tag="PXD81_000544"
     CDS             complement(11785..12789)
                     /gene="fbp"
                     /locus_tag="PXD81_000544"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005605321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HDL5536608.1"
                     /translation="MKTLGEFIIEKQAEYPGAKGELSGILSSIRLAAKIIHREINRAG
                     LSQDILGTAGSENVQGEAQMKLDVFANETMKKALLAREDVAGFASEEDDNFVAFDNER
                     GRNAKYILMTDPLDGSSNIEVNVSVGTIFSIYRRVSPIGTPVTQEDFLQEGRKQVAAG
                     YVTYGSSTMLVYTTGNGVNGFTYDPSLGLFILSHPDLKTPTEGKYYSINEGQYVTFPQ
                     GVKRFIKYCQETDEAIKRPYSSRYIGSLVSDFHRNLLKGGIYIYPTSTVYPKGKLRLL
                     YECNPIAFLAEQAGGMATDGINPILDIKPTELHQRVPFFVGSTSMVKQAGEFMQEFAK
                     "
     gene            12913..13704
                     /locus_tag="PXD81_000545"
     CDS             12913..13704
                     /locus_tag="PXD81_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HDL5536609.1"
                     /translation="MNNLRIKMISPEIALAKYNELDVLEINHSKVKAKIALQGAQLLS
                     WQPKNTQQDVLWLSEIEPFQTGVAIRGGIPICHPWFGTDKKPAHGTARIQAWNLLEHT
                     SSADGIRVVLGLENQAKLEMILGEVCELYFTHLAPEPAQAALHTYFQVGDITKTEVQG
                     LATQCFDKLTNQEIEVPSPRKISENVDCIYPAQAVNFIQDFANQRTIKVEHINATETV
                     LWNPWHNTVSAMTETAYQNMVCVETARINTLLQQNETLGVRILVR"
     gene            complement(13772..14461)
                     /locus_tag="PXD81_000546"
     CDS             complement(13772..14461)
                     /locus_tag="PXD81_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015674134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HDL5536610.1"
                     /translation="MLQLRKANERGDGSFSWLKSYHTFSFANYYDLKHIHFSHLRVIN
                     EDFIAPNHGFGKHPHDNMEILTYMLKGRVAHEDSMGNKTEVKAGEFQIMSAGTGVFHS
                     EINPDPNETLHLYQIWIIPNQKDVTPRYNQDRFAPKEGATLILSPNAEEGSFKIYQDM
                     KLWRYQYPTAKSEQLALEPNRSYWLQVVKGNLNINGVELSTSDALGIRQEKALEINTQ
                     DDVEFLLFDLV"
     gene            14654..15319
                     /locus_tag="PXD81_000547"
     CDS             14654..15319
                     /locus_tag="PXD81_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HDL5536611.1"
                     /translation="MNFTTYFSDENKRRSLILLSLFHIFIIAISNYLVQISFEVQVPL
                     TEFSFPTTWGTLTFPFIFLATDLTVRVFGADLARKIIFVVMFPALVISYVISVLFFET
                     QFQGFSALTEFNFFVFRIALASFAAYLVGQLLDVLVFNSLRQGKRWWLAPTCSTIFGT
                     LIDTFVFFAIAFYKSSDEYMAEHWFTIGSVDYAFKLFVSLLLFLPLYGVILNFIMKKL
                     DLK"
     gene            15405..16208
                     /gene="map"
                     /locus_tag="PXD81_000548"
     CDS             15405..16208
                     /gene="map"
                     /locus_tag="PXD81_000548"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HDL5536612.1"
                     /translation="MNNIPLRNEEEIQKLRVACKLASDVLVMIAPYVKEGVTTGELDK
                     ICHEYILANGATSASLGYHGFPKSVCISLNEVVCHGIPSDDKKLKKGDILNIDVTVIK
                     DGYYGDNSMMYVVGEPSVRDKRLMAVTLESLYVGIRTVKAGVRLKEIGTAIQKYVEKE
                     GFSVVREYCGHGIGTEFHCDPQVVHYATDDGGVVLKEGMVFTIEPMVNAGKKEVRLMG
                     DGWTVKTKDRSHSAQYEHQIVVTKDGCEVLTIRDEEIAEGRISRIMHNS"
     gene            complement(16324..17738)
                     /locus_tag="PXD81_000549"
                     /pseudo
     CDS             complement(16324..17738)
                     /locus_tag="PXD81_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005605131.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sucrose transporter subunit IIBC"
     gene            complement(18045..18695)
                     /gene="thiQ"
                     /locus_tag="PXD81_000550"
     CDS             complement(18045..18695)
                     /gene="thiQ"
                     /locus_tag="PXD81_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005608553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein"
                     /protein_id="HDL5536613.1"
                     /translation="MIELDLHFTYEQMNMHFQLSVKKGERIAVVGESGAGKSTLLNLI
                     AGFEKAESQQSQLWLNQKNHLDTEVAQRPVSMLFQDNNIFPHLTVEQNIGLALVPSLS
                     LNPKQKAQVKQIATQMGIETFLQRQADQLSGGQKQRVALARTLLRDKPILLLDEPFSA
                     LDPQRRAELQQIVFDICQSRNLTLLMVTHQLSESLFLFNRVITVADGEIVSDLSPL"
     gene            complement(18692..20266)
                     /gene="thiP"
                     /locus_tag="PXD81_000551"
     CDS             complement(18692..20266)
                     /gene="thiP"
                     /locus_tag="PXD81_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease"
                     /protein_id="HDL5536614.1"
                     /translation="MLSTLTKTTAWFIYFSIIALYVFSLWALMDYHQNDTLFVFSDIT
                     ATVAYSLLQASLSAVISLFLGSLLARSFFYLDFKGKNLLYKTISFIWALPSLVIIFAV
                     IGVFGNSGWLAKLFQQLGWNWQFNLYGLHGIILAHCLFNIPFVMKYYTGGLQLTPSSR
                     FQLAAQLNLTGWNYIQIVELPIIKTLLPYMFMTVFLVCFTSFPIVLMLGGSPKYSTLE
                     VAIYQAVTFEFDFAKALILIGVQFVVGIGLQLVMNYISTSALKKLKQQPNRVEIWKPK
                     PTGLAKLGLQAVIFLQSFAIILPLVSVIWAGLSVSKLSERLLNPTLWQATQYSLYLSL
                     IASIFVITISYLIALEARQLLYTKAKLRYSILTAITLFPLLLPIFLLSVGLFILLMNS
                     ELSSEALLLLVGICNGLTLLPFIYPMLFSAMWNSLTSHDKLAQGLGLKGLRRWWIVEK
                     NQLIRPLVTVFALAMSSSLGSFTVIAFFGNADFSSLPYLLYQQLGSYRTEDAAVTALI
                     LMLFALLPFLFSKDKL"
     gene            20527..>20723
                     /locus_tag="PXD81_000552"
     CDS             20527..>20723
                     /locus_tag="PXD81_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HDL5536615.1"
                     /translation="MYSKDVVITAPNGLHTRPAAEFVKAAKGFASDITVTSGGKSASA
                     KSLFKLQTLGLTQGTTITISA"
BASE COUNT         5811 a         4124 c         4564 g         6224 t
ORIGIN      
        1 atcatcaaac gcaccgcaaa gagaaacgtt ctccggacgt ggtgcattta ttatggcggc
       61 tattggttcc gctgtcggct tagggaacat ctggcgtttc ccttatacta cttatgaaaa
      121 cggcggtggg gcttttattc tgccgtatat cattgccctt ttgaccgcag gtttaccgct
      181 gctgttctta gattattcta tcgggcataa atatcgtggt ggtgcaccgc tcgctttccg
      241 ccgtttcagc tcgaaatttg aaaccttcgg ttggtggcaa gtgctgatca acgtgatcat
      301 cgggatttat tatgcggtgg tactcggctg ggcagcaagt tacacctact tctcattcag
      361 cggagcgtgg ggcgataaac cggtggattt ctttatcggt gaattcttaa aaatgggcga
      421 tattactcaa ggcgtgagct ttgaatttgt cggtatggtt acagggccgt taattgcggt
      481 atggctgatt gctattgctg tgttggctat ggggatcaaa aaagggattt ctgctgcctc
      541 aaacctgcta atgccgcagt tgatcgtgat gttcttagtg ttggtcggct attcattaac
      601 cctaccgggt gctgcgaaag gcttagatgc gttatttacc ccaaactggg aaaaattggc
      661 tgatccaagt gtgtggattg ccgcttacgg tcaaatcttc ttctcactct ctatctgctt
      721 cgggattatg attacctatg cttcatacct gaaaaaagaa accgatttaa ccggttcagg
      781 cttagtggtg ggttttgcga acagtggttt tgaattatta gcgggtattg gcgtatttgc
      841 ggcactcggc tttatggcaa cggctgcagg gcaagaagtg aacgaggtag caaaaggcgg
      901 tatcggcttg gcattcttcg ctttcccaac cattattaac caagcaccaa tgggggcatt
      961 agttggcgtg ctgttctttg gttcactcac ctttgcggca ttaacctcat ttatctcggt
     1021 aattgaagtg attatttcag cggtgcaaga caaactcaat tcaggtcgtg tgaaagccac
     1081 gctactagtc ggcattccga tgatggtggt atctgtcttg ctattcggta caaccaccgg
     1141 tttaccaatg cttgatgtga tggataaatt cgtaaaccaa ttcggtattg ttgcggtggc
     1201 gtttgtttcc ttgattgcga ttgtcaccag cgagaaatta agcacccttg aaaaacacct
     1261 gaacgaagtt tcttcattca aagtcggctt tatctggcgt ttatgtatcg ttattacctc
     1321 cggcgtactg gcatttatgt tattcagcga aatcgccaaa gtgtatcgtg aaggttatga
     1381 aggctaccca agctggtttg taaacacctt cggctgggga atggcaatcg gcttagtggt
     1441 ggtggcttat ttgctatcaa aaatggcgtg gaaaaatgaa ccaaaaggag atgagtaatg
     1501 agttcagcag caattattac catggtggtg gcaatgacga ttatctgggg cggtttactt
     1561 tttgcgatta aacgtctgcc gaaagaagaa caataatttg caaatttttg tataaaaaag
     1621 cccgcttgtt agcgggcttt tggctatacg gtaatcccaa atttctcttc cacaaaggct
     1681 tggaaatcgg tgcaaccgcc aacgtggtta ttatccacaa aaatttgtgg tacagttgca
     1741 accggcttgc caacacgagg ctctaggtct tgttttgaaa tattttcagc gatcatatca
     1801 ataaatttaa aatcaaaatc gcttaattct tgtttcattt tctcggctaa tgtttttgca
     1861 cgcacgcagt atgggcaaga taaacgtcca taaatttcaa caaacatata atgttccttc
     1921 tataaagtaa aagtgagcga attgtccgtt aaacggctct gtgtaagtgt aacaattttt
     1981 tcaacctttt aggataaatt tcttcaccat cacggcaagc agttaatgtg atatactggg
     2041 gcaaatttac aagtagagaa acagagatga cccaattaat gacgtttgag gagcttgatt
     2101 taagccctca attacttaaa gccttagcac acaaaggcta taaccgccca accgctattc
     2161 aacaagccac gattccggct gcgttagaag gacgtgatct gctcggttca gcccctaccg
     2221 gtaccggcaa aaccgccgct tttttactgc cggcaattca gcatttatta gattatcctc
     2281 gccgcaaacc aggggctcct cgcattttaa ttcttacccc aacccgagag ctggcaatgc
     2341 aggtggcgga gcaagcccaa gcactggcta aatttactca gttgagcatt gcgactatta
     2401 ccggtggggt ggcgtatcaa aatcacggtg aagtctttaa taaaaatcaa gatattgtgg
     2461 ttgctacccc gggtcgttta atgcaataca ttaaagagga aaattttgac tgccgagcgg
     2521 tggaagtgct gatttttgat gaagcagata gaatgttgca aatgggcttc gggcaagatg
     2581 cggagaaaat tgcggcagaa acccgttggc gtaagcatac ttggttgttt tcagcgaccc
     2641 tcgagggcga gttattagag gattttgccg agcgattatt aaatgatccg attaaagtcg
     2701 atgccgaacc aagtcgccgt gagcgtaaaa aaattcagca atggtattac cacgccgata
     2761 atgtagagca taaaaccaaa ttattagctc gtattatcag cgaattttcg gtggaaagag
     2821 cgattgtgtt tgtccgccgc cgagaagatg tgcgagaact cagcgaaacc ttgcgtaagc
     2881 ggggaattcg ttctacttat ctcgaaggtg aaatggctca gacccaacgt aaccaagcta
     2941 ttaaccgttt aaaagaaggc gtagtgaatg tgttagtggc aaccgatgtg gcggctcgag
     3001 gcattgatat agacgatatt gatttcgtgc tgaactacga tttaccttat agtgcggata
     3061 cttacttgca ccgtattggg cgtaccgctc gtgctggcaa aaaaggttcg gcaatttcat
     3121 tggtggaagc tcacgattat aaattactcg gcaaaattaa acgttacacc gaagaagtac
     3181 taaaacctcg cattatcgag ggcttagagc ctcgcaccaa agccccgaaa gatggcgagc
     3241 taaatgtgac cagcaagaaa gaaaaagcac gcattaagaa gaaaaaagag gcgaaaaaag
     3301 aagcggcaaa accaaaagta aaagtccgcc acaaagatac caaaaatatc ggtaaacggc
     3361 gtaaaccaag tagtgaatcg gctgcggtta aacaggcata atgatacaag cggttatttt
     3421 ctgaagattt tttgcaaaaa tttcatcaaa aataaccgct tgtttatatc taccttactg
     3481 ccatttggaa cagaatcact tctgcttggc agctgaaagt gatccacatt ttcatcagaa
     3541 acccttctgc aatttgtgta atttcgcttt gaatttcaca aggttcggtt tcgaccgatt
     3601 ttgctttttg tgcgaaaaat tcgaccgctt gttgagccag ttcttcgctc tcatacaccg
     3661 cttcaaaggc aatttggcgg tctgcattat ccaacataga atcgcctttt agccggcaca
     3721 tattatgtgc ctcagccgct ttttgttgca tcgttttgct catattacac cttatccgta
     3781 atttgtttta accaagtgta ggattgatgc tgtttttgct catcgaaaat gtagtttacc
     3841 gccataattt catcaatttc cactcgtgct tttagggctt taagttgttg ttcaacgctt
     3901 tgcgggctac caattaagga gcattcggtc atttggcgga caacggtttt ctcttgtcgg
     3961 tatagagcat tttgctcaaa ggcaaccggc ccgaaatgcg gcacaatttc tgctttcaca
     4021 aaattttccc acactttgtc gtcagattcg agcggtggtt gcagattttg ttgggcattg
     4081 gtcactacat ttaagaaaaa ctgggtttgg gtggtcgcca gttgtgttgc ttcttcatcg
     4141 gtttctgcca caatggcatt cacacctaaa atcacataag gtttatctaa cacatcagag
     4201 ggcttaaaat gtgagcggta aatgcggatt gcatcgtcca taaagcgagg ggcaaagtgt
     4261 gaagcgaagg cgtaaggcaa gcctcgttca gccgctaaat aggcactttc agggcttgag
     4321 cctagaatat aaaacggcac atttagccct tgtgccggat aggcggaaac agggtttgta
     4381 ccgtcaaagt agccttttaa ttcgctgatt tcatcgggga aaggcaaact ctgggcgtaa
     4441 ggcagaggcg gttcgcatat cggtgccggg ggctcgccca agccccagtt ctactcggtt
     4501 tgggtagagt gtttcaagcg tgccgtattg ttctgccaca atataagggc tgtggttggg
     4561 taacatcacg ccaccggagc cgactcttaa tttttcggtg tgggagaggg cgtgttgaat
     4621 caataattgc gtggcggaac tggcaagatt tttcatatta tggtgttcgg caatccaata
     4681 gcgttcaatg ccgatatttt ctgcgtgttt tgctaatttc accatagaat cgaccgcttg
     4741 tttggcagtt tgcccttcac gaatcggcac aaggttgaga atggataagt ttgtcatttt
     4801 gttctccgag tttgcatata aaaaaggctg aatagcttta ctattcagca cttgtaaaac
     4861 catctcctta atatggggac gattatttct tactcaagaa gaccacagcg tcaatatagc
     4921 gttggataaa atcattggtc gagcttgaat cggaaaatcc ttctagatta agctggtatt
     4981 gaccattcac gaaaaacgcc ggtacaccac gaatttggaa gtcttcagct aattgaactt
     5041 gtttattgac taaaccgttt actgcaaagc tgttgatacc gctgtcaaac tgttctgcac
     5101 taatgctgtt agcgatgaag accgcacgaa tatcgtccat tgatttaaac gcatcttttt
     5161 gagcggcttc aaacaaggcg gtttttactt tatcttccgc tcctaatgcc attgccaatg
     5221 cccaagcacg ggttaagttt tcagattggc gacctaagaa attgacgtga tattgcacta
     5281 atttgctgtc tgcaggcaat ttcgctttga tttggcttgg aattttgtaa tttagctcaa
     5341 aatcgtaaca gtgggggcag tagaatgaga aaaattccac cacttctttt tgagccgatg
     5401 gggctttacg cacctcaata tactctttgc cggcagttgg atctgctgca actgcggttg
     5461 cgttaaatgc aaaaagggca gaaagagcaa ccagtacggt ttttaatgca aattttttca
     5521 taatgattcc ttcaaaaata tgataagtga gaaataagac aaacttaatc gtttaaaatt
     5581 ccacgagctc ggagcaatgc ggttttaaaa tcatcttcat aatctttttt aatgcccgga
     5641 atcggctcaa atttatcggc gtttcgcatt ttgagctgat aaatcaaaac ctcgtcacgc
     5701 agggcatgaa aattatccgg ctcgcttact tccaccgaaa gctgttgcag aatatcaatt
     5761 aagtgaagat cggggttttt ccgccaatgt tcgccaagca gttccaatag ctcttctaaa
     5821 cgtttgcatt tcatagtatg cctcttaaaa aacttgttga atagtaagcg gaataatata
     5881 cttttagccc tcatttggca aaaaaattca ggtttggaga gggaaatgga agagaaaatt
     5941 tcagcggtaa ttttagccgg aggtttggct cggcgaatga atggagcgga aaaagggttg
     6001 caactgttag atcaaaaacc gttgatttcg cacctgttag cccgactttc gccacaaatc
     6061 gaggatattt ggctcaatat caatcgttcg gtggaacaat atcaacaact ttaccctgaa
     6121 ttaccttatt accaagacag cttgcccgat tttcaaggcc cactaagtgg aatgctttcc
     6181 gggttcgagc agattgagag cgactattta ctgtttgtgc cttgtgacac gccgtttatt
     6241 ccaacccaac tattgcacaa gttacacacg gcattacgca ttaatcaggc tcaaattgcc
     6301 tatgctcacg atggcgaccg cccacaccca acggttgcct tggtgcatcg ttcggtggcg
     6361 gaaaatttaa gagcgtattt agcctccggc gaacgccgat tattgtggtt ttttcaaacc
     6421 caaaaaagtg tggcagtgga tttctcggca tataaagccg catttcgcaa ttttaattgt
     6481 ttagaagatt taacggcttt caatccatat cccatcaaaa ccctggcgat tactggctat
     6541 agcggcacag gcaaaaccac cttattggaa aaattaatcc caaaattgac cgcttgcggt
     6601 attcgggttg gcttgattaa gcattcccac cacaatgttg atattgataa aaaaggaaaa
     6661 gacagctatc gcttgcgaga agcaggagca aacccgacta tggtcgtgtg tgatgaacgt
     6721 tgggcattaa tgacggaaac taagcaggca gcagaaacta aacaagcggt cgaattttca
     6781 caattaattg caaaatttga cccagagacg gtagatttag ttttagtgga aggctttaag
     6841 cacgaaacaa tgccgaaaat tcaactacac cgtaaagaaa ttgaaaaacc gttgccggcg
     6901 ttagaccagt ggacaatcgc gaccgcaacc gattatccgc ttgagcggga aaatcggttg
     6961 gatattaatg atgttgaaca gcttgctgat tttattcggc agtggctaga ggcaagcaaa
     7021 gagccggcta attaattgac taaaaagccc ctaaagtaag cgattgctta gtttaggggc
     7081 taatagcaat taccagctat atcccagttt gaagccggca aaatgttgtt tgccgatttc
     7141 gttacctttc aatagaccaa ggtgagagct taattgccat tgaccttttg taacacccac
     7201 gcccacttca gttttcagat aacgtccgaa acgttggcta aaggtattgt cattgactgc
     7261 cacactcaag cgtttgctgc tggcatcgac ataatgtgcg gcaaggctag gctcaacttt
     7321 ccagctggag aggtcaaagg ccttgctgac tttcacacct gcttggtagg ccataaagtg
     7381 tgctttcggg ctgcggactt ctacttcacc cagtttgtaa ttttgtgcgc tgaagcgata
     7441 gtaacgtgta ccaacagcag gctgaacttg tacgccggct aagtcgaagt tatgtccaag
     7501 atttgcccca atcgccataa tatgacggtt aaagcggttc tccccgaaca ggtcgatacg
     7561 gtttttcgct ttaccgtagc taccgtctag gctgacgaaa gtgccgtttt ccgaatgcca
     7621 tttaccatat aggcttgcca tcagtaagtt ggatttaccg ttcactccct catcaaattc
     7681 cgcattagcg tggttgcggg atagcatcac accggcattg ataccgtttg caagcggtaa
     7741 ttctatgcct aactgagtga ggttggtttg ttgctcatac gggcgatata aatccgattg
     7801 gtgttcggtt ttttgatgtt cgatactgct ccaaacgtgg aatttatcgt gttttgcgaa
     7861 cagttggcga tctaagtctt gcccgatttg caacgcagaa tttgcctgtg ccgatagctc
     7921 ggaaagagcg gtattggcat attggcttac ccagtcgctt tgttgaatat tactactgtc
     7981 agtgtctgcg tgtgcttgta gatgctcaca atttgcacca ttctcttggc aaagttttag
     8041 tgcctcagcg tttaaaattg cttcactatt tccagcagtt atacgtaagg tagctaattt
     8101 tgcttctact tgttttgcaa tattttgtgc agtttcaacc gcttgttcta cgttagcaat
     8161 ttgcgcagcg gatgctttag cattgttttc tgctgtagtg agtgcagcac tcgcttgtgt
     8221 gactttctgt tttgctaccg taatttgccc ttggatttgg cggtaatacg ttggatagta
     8281 acggcggtag taatcagcat attgttgcaa acgggttaat tcgctatttg cttggctaag
     8341 ctgagattgt gcgtttgcag cggcttgttt agcatttttc tctgcttggc gctctttctc
     8401 ggcttctgcg ttcaaacggc tgatttcctg tttttgttta tccgcttctg caatagcatg
     8461 gtctaattcc gctttcgccg ttgcaatcga ctgattccga tctttagcat cttttaacgg
     8521 attgtataag cgatagccgt tgcttcgatt tgccagcaca taacgatatg cacctaaatc
     8581 aacataaccg ttttccagtg aaactttagc tttatcagcc tgagcctgat gggttaaggt
     8641 taataaactt aacgggctaa ccacttcagc ttctttgcca ctgttgcgta atgcaagagt
     8701 gaaagtcccc tcggcgagac catttacttg taggtgatcg cctttgccag cagcaatatc
     8761 ggttaagtag ttgattttgg cattaccttg gaaattaccg tcaataatca acgtattaaa
     8821 acgagtcggt atctcgcctg ttacaaattt ttcattcagg ttgatggttg aaccgttttc
     8881 tgccactaaa ttgccagtac ggctatcccc agtgtttgtc cagcttgctt ggttagctaa
     8941 acgaatgctc gttgttgctg ctgcttgaat ggtgccaatc aggttcgctt tgccgatgtg
     9001 taattggctc tgttctgcca agctgacatc accattaatt tgtgttgtgg gcagtgttgc
     9061 aaaaatttca ttagaaacga ccgcttgtgt atcacagctt gttttaccag tgtactcaga
     9121 atagtagcaa cttggagttt gctcgttgat aaagcctaaa tgtagctgag catggtcatt
     9181 cgctgacaag tttgcttgca ggtttgagac attacgacca acataaagct gagcgtattg
     9241 gtttagctgc atattatcag ccttaaattg acgattttgc caatcatcgg cataaactac
     9301 atcttgctta ttgatcgcat cataagcgtg aggggtcggt ctgccggaaa gtagcacttt
     9361 acctcctttc acagttagat cgccgtttaa gttcaaacca ccagagaagg tataaatact
     9421 attgagcttt tcttgctcta ttggtgcgtt tttacgggct aatactttgt tgatcgcttg
     9481 attgcgatct gcagctaaaa attcccaatg ctcgctgctt aagccaccgc gaggaacagg
     9541 ttgttttgga tcacctttta atgcaaagta ttcccgaatt ttcgttttcg gattggtgaa
     9601 cagataaata tctgcaccag ctactagccc cttaccccaa gttacttcag aggcagttgc
     9661 cggtggtgta gaggcttttg gatcatacac cacattcaaa cgaccgttat gctgggtgtt
     9721 atctgtttcg ccaataaaac cattaaagct attgtagcga tgctccagac ctttttgcgc
     9781 gagtacttgt tttactgctg catccttgtt agaagacagg aaggtccaat gggcattact
     9841 tgcgccattc gttggcatat attggtttgg attgcctttg agcgtaaagt aatcggtacg
     9901 gttattagca tgtgggttga tatattcata aatatcagca ctgttttctt tccacgttcc
     9961 ccattttaag tccgttgctt cagggggctt cgggccatta atggtgagtg ttgcagcttt
    10021 ttgtaggtga ttattaacaa tacgcgcacc atcatcacta ttttgaatac gattaaacgc
    10081 aatgttgttg ccgttgagat ctaaacgacc accacggaag ccaaagtaaa tcttatttgg
    10141 atcgacttga tcagcactgt ttaatacaac ggttgaacgg ccgctcacaa taccaacttg
    10201 gttaaatgca gattttttgc cattttcacc cgcttgttgg gtcaaaatga cagtgccttg
    10261 tccaacacta atgtcaccaa ggttttcgcc tttaccgtta atatgtagtg tcccttcgcc
    10321 caatttagat aaacggtcac cttgtgggtt tttgacttgc caattaactt gtttaccttt
    10381 tgccactaca attccagctc ctacccagct ttgatcattt tcagggcgaa ctgttgcatt
    10441 agcattgaaa tttaatgcgc cagcaccttg gtcaatatcg gtttttaata cgatagtgct
    10501 atcttgtcct tcaatgttta gggtttttcc ggtgttatct tgcggaagtt gtagctgagg
    10561 tgtgccacca tagcctaatg gagaagcgct attgtcttta attgaacgat cagcaagcga
    10621 aaccgttaag tttttccctt tattaccaat gctactgtct ttaccctgtg cagaccattc
    10681 aaaaacggca ttttgttgcg cattggttaa tgtgccggca atatcttcgg caaattgttt
    10741 atcgtgataa tcctgacgga taataccggc acggttgtag tagttgtttg caggcgtacc
    10801 aaaggtgtaa gtagccagta gtagccagcg tttcgttttc tgatcgtagc catacacacc
    10861 tgaaccgcta tcaccacgag taccaaatgc agttaatgct ccatatgggc tattgtttaa
    10921 tgcatttcct ctaaaatcta gccagtaatc ggaattatgt accgcgattg gtaaatgaac
    10981 tcccccggtt aaatattggt aagatgttga aatttggttg tctttatgct cggtattttt
    11041 gactatttga cgaccagagc ctaagcgagc aaagtgagta aaacgactac tatcttggta
    11101 ggctgaagca ttattaccca gatcagtcac tgttgcaggt gcgacctcag tgactaattt
    11161 attgagacgt ggcacttgat aatcttgatg ttgcccacct tcaacaattc catagtcgtt
    11221 acgatcaacg actttgtaag tgtagtggtg tgcatcggca ttagtgcctg gggcgccaaa
    11281 ttccacagta ttgtattgtg tattatgggc aacgctgact aaatattgcg gtgcaactag
    11341 agttgctatc cctaagttac ggtttgcagc actaaaatct ggcatcggta catttgggaa
    11401 gagtgcatct aatgtattgc cacttttatc tttaatttca atattgcttg cacctacggt
    11461 gaatttaccc ttattctccg caaaatcacg gtagtattga agatcgatat catctctgaa
    11521 aacagaggct gttgcagata aactaacagc aaatgccaca aagctggcaa ggtaagaaag
    11581 tggaaagttt ttatttatca taaaatgtta ataaattgct aatattaatg gagtaaaaag
    11641 gtggggtatt ctatagataa ttatttattt ggcaagaggt cttacctgtt ttttatacaa
    11701 atttacaatc ttaagatagg caaaagcagc tacggaatac gcagctgctt tgttgaaagt
    11761 tttattgagt gataagcagg agcattattt tgcaaattct tgcataaatt cgcccgcttg
    11821 tttgaccatt gaggttgaac cgacaaagaa cggtacacgc tggtgtagct ctgttggctt
    11881 aatatctaaa atcggattaa taccatctgt cgccattcct ccggcttgtt cggcaagaaa
    11941 cgcaatcggg ttgcattcat agagtaaacg caatttccct tttggatata cggtggaggt
    12001 tgggtaaata taaataccgc cttttaataa attgcggtgg aaatcggaca ccagcgagcc
    12061 gatatagcgt gaagagtatg gacgtttgat ggcttcatcg gtttcttggc aatacttaat
    12121 aaagcgtttc acaccttgtg ggaaagtaac atactgccct tcattgatgg agtaatattt
    12181 tccttcggta ggggttttta gatcagggtg tgaaagaata aataagccta atgacggatc
    12241 ataggtaaaa ccgttcacgc cattgcctgt ggtgtaaacc agcatggttg aagaaccata
    12301 agtaacataa ccggcggcaa cttgtttacg cccttcctgc aagaaatctt cttgtgttac
    12361 tggggtgccg ataggggaga cacggcggta aatagagaag attgttccca cagaaacatt
    12421 cacttcaata ttggaggagc catcaagcgg atcggtcatt aaaatatatt ttgcatttct
    12481 cccacgctca ttatcaaagg caacaaaatt atcatcttct tccgatgcaa agccggcaac
    12541 atcttcacgt gcaagtaacg cttttttcat cgtttcgttg gcgaatacat caagtttcat
    12601 ttgggcttca ccctgtacat tttcagaacc tgcagtacct aaaatatctt gacttaaacc
    12661 ggcacggttg atttctcgat gaataatttt agcggctaaa cgaatagaag ataaaatccc
    12721 acttaattcc ccttttgccc ctgggtattc tgcttgtttt tcaataataa attcgccgag
    12781 tgttttcatt tttttctcct tcgctatgtt agaatgccct tattataacg attaccttta
    12841 aagaggtagc ggtttaagct ttaattctgt gatccacttc acattttatc tttcaagatg
    12901 aggagcaata cgatgaataa tctacgaatc aaaatgatca gccctgaaat agcattagca
    12961 aaatataatg aattggatgt tttagagatt aatcactcca aagtgaaggc taaaattgca
    13021 ttgcaaggtg cgcagttact gagttggcaa cctaaaaata ctcagcaaga tgtattatgg
    13081 ttaagtgaaa ttgaaccttt ccaaacaggt gtggcgattc gtggcggcat tccgatttgc
    13141 cacccttggt ttggtacaga caaaaagcct gctcacggca cagcccgtat tcaagcgtgg
    13201 aatttgctgg aacatacttc ctcagcagat gggattcgtg ttgttttggg gttagaaaat
    13261 caggcaaaac tcgagatgat actaggcgaa gtctgtgagt tgtattttac tcacttagca
    13321 ccggagccgg cacaggctgc attgcatact tattttcagg tcggtgatat tacgaaaacg
    13381 gaagtacaag gcttagcaac acaatgtttt gataaattga caaatcaaga aattgaagtg
    13441 ccatcacctc gcaagataag tgaaaatgtg gattgtattt atccggcaca agcggtgaat
    13501 tttattcaag attttgcaaa tcagcgcacc attaaagttg agcatatcaa tgctaccgaa
    13561 accgtacttt ggaatccttg gcataatacc gttagtgcga tgacagaaac ggcgtatcaa
    13621 aatatggtat gtgtggaaac agcacggata aataccttac ttcaacaaaa tgaaacgcta
    13681 ggagtgagaa ttttggtgcg ataatgttta atgagagcaa gcagctgaat tagtaatatt
    13741 ttttacaaat ttaaccgctt gtctcttgta tttacactag atcaaacagt aaaaattcca
    13801 cgtcatcttg agtgtttatt tcaagtgctt tctcttgacg aatgcctaag gcatcgctgg
    13861 tggaaagttc aacgccattg atgtttagat tccctttcac cacttgtagc cagtaactgc
    13921 ggtttggctc taatgccagt tgctctgatt tggctgttgg gtattgataa cgccacagct
    13981 tcatatcttg gtaaatttta aatgaacctt cttctgcgtt tggcgaaaga atcaaggttg
    14041 ctccctcttt cggggcaaat ctatcttggt tgtagcgagg ggtcacatct ttttggtttg
    14101 ggataatcca aatctggtaa aggtgtaagg tttcattcgg atctggatta atttctgaat
    14161 ggaaaacccc ggttccggca gacataattt gaaattcgcc agcttttact tcggttttat
    14221 tacccatact gtcttcgtga gccacacgtc cttttaacat ataagttaaa atttccatat
    14281 tatcgtgcgg gtgcttgcca aaaccgtgat taggtgcaat gaaatcttcg ttaattaccc
    14341 gtaaatgtga aaaatggata tgttttaggt cgtaataatt ggcaaaggaa aaagtgtggt
    14401 agcttttaag ccagctaaaa gaaccatcgc ctcgttcatt ggcttttctc agttgtaaca
    14461 ttatctattc cttatgtaag tgttaagttg tgccatttta ctttatgtca aaaatagata
    14521 aaccctaatt taatagatta attgtttcat tttttgaaat aatatgagct tgatttttat
    14581 gggaaaatgt atagaataat gcccccttga gttcccttac ctcaagtttt catttaacga
    14641 aaaaggatac aatatgaact ttactactta tttttcggat gagaacaaac gtcgttctct
    14701 aattttactt tcacttttcc atatttttat catcgcaatt agcaactatt tagtgcaaat
    14761 tagctttgaa gttcaagtac cactaacaga atttagcttt ccgacaactt ggggaacgtt
    14821 aacattccca tttattttct tagcgaccga tttaaccgtg cgtgtatttg gtgcagatct
    14881 tgctagaaaa attatttttg ttgtgatgtt tccggcactt gtcataagct atgtgatttc
    14941 cgttttattc tttgaaaccc aatttcaagg ctttagtgca ttaactgagt tcaacttttt
    15001 tgttttccgt attgcactgg ctagctttgc agcttattta gttgggcaat tattagacgt
    15061 tctagtattt aatagtttaa gacaaggtaa gcgttggtgg cttgctccaa cctgttcaac
    15121 catttttggt actttaatcg atacttttgt attctttgct atcgcctttt ataaaagtag
    15181 cgatgaatat atggcagagc attggtttac tattggttcg gtcgattatg cgtttaaact
    15241 ctttgtgagc ctactgctat ttctaccgct ttatggtgtg atattaaatt tcattatgaa
    15301 gaaacttgat ttaaagtaga aatctaaact gaggtttggg gattgtgctg aaaaaaggta
    15361 taatcccctt actttttatg gcagatatag cagagagcaa tgtaatgaat aatattccat
    15421 taagaaacga agaagaaatt caaaaactac gagtagcctg taaactggct tcagatgttt
    15481 tagtaatgat cgccccttat gtgaaagagg gcgtaacaac tggtgaatta gataaaattt
    15541 gtcacgaata cattttggca aatggggcaa cttcagccag tttgggctat cacggcttcc
    15601 caaaatcggt gtgcatttcg ttaaatgagg tcgtttgtca cggtattcca agtgatgaca
    15661 aaaagctgaa aaaaggcgat attttaaaca tcgatgtgac ggttatcaaa gatggctact
    15721 atggcgataa ctcgatgatg tatgtagtcg gtgagccaag tgtgcgggat aaacgcttaa
    15781 tggcggttac gctagagtcc ctttatgtcg ggattcgcac cgtgaaagcg ggggtgcgtt
    15841 taaaagaaat tggtacagcg attcaaaaat atgtggaaaa agaaggcttt tcggtagtac
    15901 gagaatattg cggacacggt attggtactg aattccattg cgacccacaa gtagtgcatt
    15961 atgctaccga tgatggcggt gtagtgttaa aagaaggaat ggtgtttacg attgagccaa
    16021 tggtgaatgc cggcaaaaaa gaagtccgct taatgggtga cggttggaca gtgaaaacca
    16081 aagaccgcag ccactccgca caatatgagc atcaaattgt agttaccaaa gacggctgtg
    16141 aagtgctgac aattcgtgat gaagagattg ccgaagggcg tatttctcga attatgcaca
    16201 acagctaatt tgcaaatttt tagcgaaaaa tgaccgcttg cgaaactcat tgttaagcta
    16261 aggcgtttaa aaacaaaagg gattaagcta acacttaatc ccttttttgt gatctatttt
    16321 tgattatttc gcttgtgctt ttttcaccca aatggctgaa atggtaaatg caactacaaa
    16381 tgaaattacc ataccgacag aatacattgc aaggctgtca ggtttaatcg acggaatacc
    16441 taagaaaccg gctgcaccta aggcaattgc tttcacatta aagaaagcga taaatgcact
    16501 ggctaaacct gaaccgatca tcgccgcaat aaaggcttgg cggtagcgta aatttactcc
    16561 gaacattgcc ggttgatacc taacagggct gaaatacctg acggcacggc taaaccacgc
    16621 actttggcat ctttcattgc aaaagctaca cctaaacagg ctgcaccttg tgcaatgtta
    16681 gacattgctg caatcgggaa gataaatgta ccgccggttt gtgctacttc agctaataat
    16741 tgggtttcaa ccgcaataaa ggtttggtgc ataccggtaa tcacgattgg agcgtagaat
    16801 gtaccgaaaa ttgctccacc gataaagcct aagctgtcat ataaccacgt taagcctgct
    16861 gaaatcgctg aacctgcttc acgacccaat ggtccgatta cagtgaaagc taaaataccg
    16921 gcgataaata atgacatcat tggcgtcacc aagttatcta aatagcttgg cacaaattta
    16981 cggaaagttt tctcaagggt tgctaacacc caagccgaaa cgatagttgg aataaccgtg
    17041 ccttgataac ccactttttc aatttctaag ccgaaaatat tccagtattt gatgttacct
    17101 tccattaagg ttttggcata gttccaaccg tctgcaagtg ccgggtgaac caataacata
    17161 ccaagtgccg cacctaagaa tggattaccg ccaaatttgc gtgtagctga gaaaccgagt
    17221 aatacaggta agaatacaaa tggtgcgtta gcaatagtgt tgatgaaatc aactaaatcg
    17281 gaaatattcg gataacggct aacaacagat tcaccttccc agaagaaacc gactgccgtt
    17341 agcattgagt gaatacccat taacaaaccg ccagccacaa tagccggaat gatcggcacg
    17401 aaaatatcgg ctaagccttt taccaaacgt tgtagtaatg gctgatttgc tgcaccggca
    17461 gccgctactt cagaggtgga catatctgcc atacccattt gtttttgcat ttgggcatac
    17521 acttcattta ccgtgcctga tccaaaaata atttggtatt gaccgggagt agagaattga
    17581 cctttcacgc cttcaatatt ttcaatcgcc tctttatcaa ttttggattc atcagcaatg
    17641 gttaagcgta aacgggttgc acagtgggca agtgtggtaa tgttttcttt gccacctaat
    17701 tttgcaatcg tttgttctgc aatttttgga tagttcattt gaatttacct ttcataaaaa
    17761 ataatcggtt gcattttaac taaatcggtt tagctaaaaa agagaagatt taaaatattt
    17821 ccaaatttgt gaagtagatc acattttggg gaagagaata gcggaatagg gaggtaatat
    17881 tagttacagt tcccctcttt gctaaagtag ggggcgagtt ctgaaaaaaa tgaataacgc
    17941 tagtatccgt gcggaacatc attcaccgtt agattgttgg aattgacatc gttgcgtacg
    18001 ccagcgtggc gtccctacat ttagaaccta gcagatctgc tttgctaaag aggggagaga
    18061 tcagaaacaa tctccccatc tgctaccgtt atcacccgat taaatagaaa taggctttcg
    18121 gatagttggt gagttaccat taacagggtt agatttctgc tttggcaaat gtcaaacacg
    18181 atttgctgta attcggctcg gcgttgcggg tcgagggcgg agaagggttc gtccaataat
    18241 aaaatcggct tatcccgcaa caaagtacga gctaatgcca ctcgctgttt ttgtccgccc
    18301 gatagctgat cggcttgacg ctgaaggaag gtttcaatcc ccatttgtgt ggcaatttgt
    18361 ttaacctgtg ctttttgttt cggattgagc gacaaacttg gtactaaagc taagccgatg
    18421 ttttgttcta ccgttaagtg cgggaaaata ttgttatctt ggaatagcat tgaaacagga
    18481 cgttgtgcaa cttcggtatc aagatgattt ttctgattta gccacagctg gctttgctga
    18541 ctttctgcct tttcaaaacc ggcaattaga ttaagtaatg tgcttttgcc tgcgccactt
    18601 tcgccaacca ctgcaatacg ttcgcctttt ttgacagaaa gctgaaagtg catattcatt
    18661 tgttcgtaag tgaaatggag atccagttcg atcatagttt atccttgctg aataagaatg
    18721 gtagtaacgc aaataacatc agaatgagtg cggtaacggc agcatcttcg gttctatagc
    18781 taccaagttg ttggtagagt aaataaggga gtgagctgaa atcagcgttg ccgaaaaaag
    18841 caatgacggt gaaactgcct aagctcgatg acattgctaa cgcaaatacg gtaacaagcg
    18901 gtcgaattag ttgatttttt tccacaatcc accaacggcg aagccccttt agcccaagtc
    18961 cttgtgctaa tttgtcgtgc gaggtgaggc tattccacat tgcggagaaa agcattgggt
    19021 aaataaatgg cagtaatgtt aagccgttgc atatcccgac cagtagcagt aatgcctcgc
    19081 ttgagagttc actattcatt aataaaataa acaaaccaac cgataataaa aagatcggca
    19141 gaagtaaggg gaatagggtg attgcggtga gaatgctata tctgagcttg gcttttgtat
    19201 agagtaattg gcgagcttca agtgcaatca aatagctgat tgtgattacg aatatagaag
    19261 caattaaact taagtataaa gaatactgag tagcttgcca taaagtaggg ttgagcaaac
    19321 gctcactgag ttttgacacg cttagtcctg cccaaatgac gctgacaagc ggtagaatta
    19381 ttgcaaaact ttgcaaaaaa ataaccgctt gtaagccgag tttagccagt ccggtaggct
    19441 ttggtttcca aatttctact cggttaggtt gctgttttag ctttttgagt gcagaagtcg
    19501 agatataatt cattactaat tgtaagccaa tgcccactac aaattgtacc ccaattaaga
    19561 tgagggcttt tgcaaaatca aattcaaaag tgaccgcttg ataaatggca acttcaagcg
    19621 tgctgtattt tgggctaccg cctaacatta atacgattgg aaaactggta aagcagacca
    19681 gaaaaacagt cataaacata taaggcagca aagttttgat tatcggtagt tccacaattt
    19741 ggatataatt ccaacctgtt aggtttaatt gagccgcaag ctgaaagcga ctgcttggag
    19801 tcaattgaag ccctccggta taatatttca tcacaaacgg aatgttaaac aggcagtgag
    19861 ccaaaatgat gccgtgcaag ccgtataaat taaattgcca attccaaccg agttgttgga
    19921 aaagtttggc tagccagcct gagttaccaa atacgccaat taccgcaaaa ataattacta
    19981 gtgagggtaa tgcccagata aacgaaatgg ttttatagag taaatttttt cctttaaagt
    20041 ccaaataaaa gaaagatctt gccagtaggc tgcctaaaaa tagtgaaatt accgcagaga
    20101 ggctggcttg aagtaggcta taagcgaccg tggccgttat atcggaaaaa acaaataagg
    20161 tatcattttg gtgatagtcc attaacgccc atagactaaa aacatagaga gcaataatac
    20221 taaagtagat aaaccatgcg gttgttttgg ttaatgtgct aagcatattg ctccaaattt
    20281 agaatataag tgaaagaagt ggtagcgaaa ttattgtaac atttccgctt aagtgtgtgc
    20341 tagaatcttg atattcgaca aatgattttt aagttagctt aaaaaacacc ctatttagca
    20401 gggtgatacg acgagttttt gcgcttttta gcgtgttttt aatttgaatt tgtagtaaag
    20461 ttgcgtgcgc aaaataaatg gattaaaaca aattttttta actttcaaat ttaagaggaa
    20521 gattttatgt actcaaaaga tgttgttatt accgctccaa atggtttaca cactcgccca
    20581 gcggctgaat ttgtaaaagc agctaaaggt tttgcttctg atatcactgt gacttcaggt
    20641 ggtaaaagtg caagtgcaaa aagcttattc aaattacaaa cattaggttt gactcaaggc
    20701 acaacaatta caatttctgc gga
//
LOCUS       DANLYI010000019        20660 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.019, whole genome shotgun sequence.
ACCESSION   DANLYI010000019 DANLYI010000000
VERSION     DANLYI010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 20660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20660
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.019"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            complement(<1..360)
                     /locus_tag="PXD81_000553"
     CDS             complement(<1..360)
                     /locus_tag="PXD81_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5536616.1"
                     /translation="MITSNLERSFHGLNIAQHNYKIFLDESGGNIGKLKHTPNDKKGS
                     NFNRTISSHIGVPPVFVLGGIAQISNANLIAPDWRKLLHLQNKELKFSKIGKNFFNKL
                     GSKELRKVLEWINENNLC"
     gene            complement(387..1061)
                     /gene="cas4"
                     /locus_tag="PXD81_000554"
     CDS             complement(387..1061)
                     /gene="cas4"
                     /locus_tag="PXD81_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas4"
                     /protein_id="HDL5536617.1"
                     /translation="MGDLQNSAEILPLVQPKFGDDKRSISLSALQHYAFCPRQCALIH
                     NEQVWAENYLTAQGQALHERVDSGEPEVRKGIRFERTVHVAAEKLGISGILDLVERDL
                     KTGELKPVEYKRGKPKLDPIDEIQLCAQALCLEEMTEQTINEGALWYMQTRHRLPIIF
                     STELRTKTLATIAEVRSLLDSGTTPPPEYSKRCKACSLIDLCQPKLLERDRSKGYVVG
                     VFGSLL"
     gene            complement(1061..1924)
                     /gene="cas7c"
                     /locus_tag="PXD81_000555"
     CDS             complement(1061..1924)
                     /gene="cas7c"
                     /locus_tag="PXD81_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004284050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas7/Csd2"
                     /protein_id="HDL5536618.1"
                     /translation="MSIQNRYEFVFFFDVTNGNPNGDPDAGNMPRLDPETSKGLVTDV
                     CLKRKIRNFIEMSYENEAGYEIYVKEKSVLNLQNKRAYEALGIESEAKKLPKEEAKAR
                     EITAWMCKNFFDIRTFGAVMTTEVNSGQVRGPVQLAFAQSIDPIIPLEVSITRMAVTN
                     EKDLEKERTMGRKYIVPYALYRVHGFISAKLAEKTGFSDEDVQKLWQALQLMFEHDRS
                     AARGEMAARKLIVFKHDSELGNQPAHKLFDSVKVERINGEKDTPAKDYDDYCISVQTE
                     GLTGVTVEELL"
     gene            complement(1934..3715)
                     /gene="cas8c"
                     /locus_tag="PXD81_000556"
     CDS             complement(1934..3715)
                     /gene="cas8c"
                     /locus_tag="PXD81_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas8c/Csd1"
                     /protein_id="HDL5536619.1"
                     /translation="MITALTRYYYRLAAQEDRLTGEAKVPSYGFSEEKIGWVLELDSK
                     GNLVNVISNLTEDKKPKPKLMSVPRPEKRTSGIKPNFLWDKTAYVLGIESNPDKATAK
                     DQPINFAEKTFEAFKQYHLDLLADSNDEGLMAIRNFLQQWQPTHFAESICPREILDAN
                     VVFKLVGTSGYIHQREAAQTLWTSLLINQSDNAEKGLCLISGKLSPIQRTHPPIKGVF
                     GGQSSGGSIISFNKESFTSFGKEQGSNAPVSEQAAFAYTTALNYLLRTEKHRLTIGDA
                     STVFWAEADNTTQAEAAEGFFAALMTPPDDEQENQKIFEELQKISKGRPLAEISPELS
                     ENTRFYVLGLAPNAARISVRFWLDTTFGQLAKNLSEHWQDLALEPCPWKTPPSIWRLL
                     LQTASLGKSENISPVLAGEMARAVISGHLYPMSLLSQLVTRIRADGDINGLRVALMKA
                     VLQRKFRKGFIKEGVPMSLNKESDNQAYLLGRLFAVLERIQSQALGELNAGIADRYYG
                     SASAVPFSVFPRLLSGAKHHLSRLRKDKGGMAVNLDKDLGEIIASLPENFPRHLSIDE
                     QGRFAIGYYHQKQSYFAKKETTESIEN"
     gene            complement(3737..4414)
                     /gene="cas5c"
                     /locus_tag="PXD81_000557"
     CDS             complement(3737..4414)
                     /gene="cas5c"
                     /locus_tag="PXD81_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas5"
                     /protein_id="HDL5536620.1"
                     /translation="MPNKIRLHIWGDYACFTRPEMKVERVSYDVITPSAARGILAAVH
                     WKPAIRWVIDKIYVLQPIQFESVRRNELGSKISASKISGAMKRKTVADLYTVIEDDRQ
                     QRAATVLKNVGYVIEAHAVLTKMAGEAETVTKHIEMFKRRAKKGQCFQQPCMGVREFA
                     ANFALIDDDEPLPECFLAENERDRDLGWMLHDIDFEHGNQPRFFRAEMKNGVIDVPPF
                     YAEEVKS"
     gene            complement(4656..6407)
                     /locus_tag="PXD81_000558"
                     /pseudo
     CDS             complement(4656..6407)
                     /locus_tag="PXD81_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250669.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
     gene            complement(6397..6858)
                     /locus_tag="PXD81_000559"
     CDS             complement(6397..6858)
                     /locus_tag="PXD81_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5536621.1"
                     /translation="MFKPHGSLDWFMAGDVPMTSCIGKVEEPLIITPGVGKYKKGYGQ
                     PFDAHREKANEAIDSASSILCIGYGFNDDHLQTHLTGKIKSGVKTLLLTRGLSENARN
                     VVKEAPNCKALIFDGDKAGTRVVSKQEETFIPNVHWWDVEFFVKEILENGK"
     gene            complement(7017..8153)
                     /locus_tag="PXD81_000560"
     CDS             complement(7017..8153)
                     /locus_tag="PXD81_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012340754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL5536622.1"
                     /translation="MLLRKAFKFEIRPNGEQVRKMKQFCGCSRFVFNKALAWQNEQYE
                     QDNHHKFSYTKLANLLPQWKKELVWLKDCHSQVLQQSLKDLESAFKNFFQKRADLPKF
                     KKKGVKESFRFPQGCKIEQKNDRLYLPKIGWVRYRNSREIVGEVKNVTVSMKCGRFFV
                     SIQTEFKQEIPTHKGGEIGIDMGIARFATLSNGEFFEPLNAFKTHKGKLAKLQKQLKN
                     KVKFSKNWQKLKDKIGKLHHKIANCRKDFLHKISTQISKNHAMIYVEDLQVANMSKSA
                     KGTAEVHGKNVKQKSGLNRVILDQSWFEFRRQLDYKTQWLGGFLLAVPPQNTSRTCPC
                     CNYTAKENRQTQENFECVECGYTENADVVGAMNVLTRGQAIVQA"
     gene            8200..8616
                     /gene="tnpA"
                     /locus_tag="PXD81_000561"
     CDS             8200..8616
                     /gene="tnpA"
                     /locus_tag="PXD81_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005720092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HDL5536623.1"
                     /translation="MKKETEIRRGRHVVFNLHVHLVFVTKYRREVFTQAILNDLHSIF
                     ATICADFEATLVEFDGEDDHVHLLVEYPPKVAISHLVNSLKGVSSRMIRKKNYPSIRK
                     KLWGNQLWSPSYFAGSCGGAPISIIRQYIEQQQTPD"
     gene            complement(8678..9271)
                     /locus_tag="PXD81_000562"
     CDS             complement(8678..9271)
                     /locus_tag="PXD81_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5536624.1"
                     /translation="MREPLLDEDELKKWLQTIFTDNLVIIVGSGLSCAEGLPGMGALA
                     DRLKERMPECLDDIDKVTWNTISDCLDSEGLEGALLKHQANETIEAAIIKITAEYVLS
                     EEQKAINKCIAENQKLKFSYLLPHISASNPKIARVITTNYDRLIEFAAEYENWGIDSM
                     MVGRYWGKHNPDLSRKLQIRDIRVKGKCPKLVYLIVP"
     gene            complement(9402..11690)
                     /locus_tag="PXD81_000563"
     CDS             complement(9402..11690)
                     /locus_tag="PXD81_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase/endonuclease Cas3"
                     /protein_id="HDL5536625.1"
                     /translation="MEEHTIQSFYAHSGKLSDHSDWQTLLSHANNVGLLAAEFAAPFA
                     SQEIARCTGLLHDLGKYSERYQKRLHGGERVNHSTAGAKIAVERWGPLGKMMAFCIAG
                     HHAGLANGSGEGKNRSTLTQRLQEQFGYGHKDLPVLDAIWQQELNLPEKLPLPPLKPS
                     KNEPFFSYAFFIRMLYSCLVDADFLDTEAFYANLEKRPLERGNYPNLTALHQQFNQFI
                     AQFRERIATQTPNTQEAQRKAEVNRLRSEILDYAVSQAKQETGLFSLTVPTGGGKTFT
                     SMAFALEHAKQYGLCRIIYVIPFTSIIEQNAAEFRKAFGELGTEAVLEHHSTFDHDSL
                     PDKSSRDKLKLASENWDTPVVVTTAVQFFESLFADRSSRCRKLHNIVGSVIILDEAQM
                     LPLNLLRPIMAAIDELARNYQCSVVLCTATQPAIHQANGFYKGFENVREIAPNPTALF
                     EKLKRTTVKHIGIQTDVELQSKLADNPQMLIIVNNRRHARALYDSAKQLEGAYHLTTL
                     MCAKHRSQTLTEIRLRLKQNLPCRVIATSLIEAGVDVDFPLVMRAEAGLDSVAQAAGR
                     CNREGKRLSVESNVWVFQPEEQWKAPPELGLLSGCMRSTVQVHADDLLNPKAINHYFQ
                     AVYEAKGKELDKPNILKACHDAGQSLNFPFQKIAEDFRMITSHLIPVIIPFDQEAENL
                     IDRLRYADQVGGILRKLQPYTVQVPEKALAELYNAGRVETINETNFGKQFYCLIGMDL
                     YDEVAGLSWENVDFLESEKLVL"
     gene            11954..13717
                     /locus_tag="PXD81_000564"
     CDS             11954..13717
                     /locus_tag="PXD81_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     accessory protein YcaO"
                     /protein_id="HDL5536626.1"
                     /translation="MSEQTFITGKDAALEDSIATFQHKLKTLGFNIEEASWLNPVPNV
                     WSVHIRDADCPQCFSNGKGASKKAALASALGEYFERLSTNYFWADFYLGQEIANSEFV
                     HYPTEKWFPIDDEAIPPMEMMDEFLWEYFDPNGEITPELLVDLQSGNYDRGIVTLPYV
                     RQSDNETVYIPQSIIGNLFVSNGMSAGNTKNEARVQGLSEVFERFVKNRIITEAISLP
                     EIPQSVIDGYPTIKASIEKLEQEDFPILCYDASLGGEFPVICVILLNPNNGTCFASFG
                     AHPNFQVAFERTVTELLQGRSLKDLDVFAPPSFNNEDVADHANLETHFIDSSGLISWD
                     LFKRDADYPFVHWDFSGTTEQEFNNMMAIFNKHNQPVYIMDYEHLGVYACRILAPGMS
                     NIYPSDDLVYANNNMGMDWREILLDLPHFHHDAETYQELLDELDEQSIDDLTRVREFI
                     GVVAEKGSAMQTLRIGELKSMLHLALGNLEQASDWANWTANMNASVFSAERNNYYRCL
                     IQSIELFLDEERDSEQYRMVFEKMYGKQAVEAAWGAIQGGNPFHGLTASDESLENMTA
                     HQKLLNAYRKVQKAKVNPIAR"
     gene            13814..14776
                     /gene="cmoB"
                     /locus_tag="PXD81_000565"
     CDS             13814..14776
                     /gene="cmoB"
                     /locus_tag="PXD81_000565"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005707797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HDL5536627.1"
                     /translation="MIDFRPFYQQIATTDLSAWLETLPLQLKQWEKTTHADYAKWAKV
                     ADFLPNSTACINLKDKVESISSKPLSEGEVKQITHHLKQLMPWRKGPYHLHGIHIDTE
                     WRSDFKWDRVLPHLAPLKDRTILDVGCGSGYHMWRMVGEGAKMVVGIDPTELFLCQFE
                     AVRKLLGNDRRANLIPLGIEQMQPLAAFDTVFSMGVLYHRKSPLDHLSQLKAQLVRGG
                     ELVLETLVIDGDINDVLVPADRYAKMKNIYFIPSVAALINWLEKVGFKNVRCVDEAIT
                     TLEEQRKTDWLENESLVDFLDPNDHSKTIEGYPAPKRAVILANA"
     gene            complement(14829..17261)
                     /gene="gyrB"
                     /locus_tag="PXD81_000566"
     CDS             complement(14829..17261)
                     /gene="gyrB"
                     /locus_tag="PXD81_000566"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HDL5536628.1"
                     /translation="MSEQTQATSYDSSSIKVLRGLDAVRKRPGMYIGDTDDGTGLHHM
                     VFEVVDNAIDEALAGHCKDIIVTIHSDNSVSVQDDGRGIPVGIHPEEGVSAAEVIMTV
                     LHAGGKFDDNSYKVSGGLHGVGVSVVNALSSKLQLTIRREGHVHEQFYSLGEPDAPLA
                     IIGNTEKTGTSVRFWPSLDIFKNKTEFEYKILSKRLRELSFLNSGVSIKLIDERDGKE
                     EHFKYEGGIKAYVEYLNEGKTHIHNTPFYLTTEKDGIGVEISLQWNDSYNENVYCFTN
                     NIPQRDGGTHLAGFRGALTRALKNYMDNSGVLKKADANIDASGDDAREGLVAVISVKV
                     PDPKFSSQTKDKLVSSEVRGAVESSMNEALSDYLAENPDDAKNIVTKIIDAARAREAA
                     RKAREMTRRKGALDLGGLPGKLADCQEKDPALSELYLVEGDSAGGSAKQGRDRKNQAI
                     LPLKGKILNVEKARFDKMLSSQEVATLITALGTGIGRDDYNLEKLRYHKIIIMTDADV
                     DGAHIRTLLLTFFYRQMPELIENGYIYIAQPPLYKVKKGKQERYIQDNDEMSQYEIDI
                     ALEGAGLYVSEDAPALSGVALENLIAQYNGVQKLIERLSRRYPTLLLNELIYSKPLSV
                     EFAKNQANMTAWSEQFVATLMAKEVGGSFYRINTLFNEERQIYEPEIIVTTHGMDSTY
                     RLDYNFINGNEYARIVALGNELNDLLSDSAYVVRGERRQEISSFAEALDWLVKESRKG
                     LTIQRYKGLGEMNPEQLWETTMDPVARKMLQVNITDAIAADKLFSTLMGDEVEPRRDF
                     IETNALYAQLDI"
     gene            complement(17393..20029)
                     /locus_tag="PXD81_000567"
     CDS             complement(17393..20029)
                     /locus_tag="PXD81_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HDL5536629.1"
                     /translation="MELQFETLEYQLQAVNATVNLFVGQPNAATEFSLKAQNDMRFVP
                     NLGLQISDEQLQQNLANLQNRQKIDRTLLVEQGKNFTVEMETGTGKTYVYLRTIFELN
                     RQYGWQKFVIVVPSVAIREGVLHTLETTKSHFNTVFDNPSVNQKFEYKSNQTSRLKSF
                     ASANHIEILVMNIDAFTKESNVINTVNESGDAPIFYIQQANPIVIIDEPQNMETEIRR
                     NAIESLSPLFTLRYSATHKNAYNPIFSLNPVQAYEMGLVKQIEVDSVLAENEVNGAYL
                     SLKEIKTGAKSWSAKIEILFNDKKAMKKKVISVKPNQDLYELSNQNEMYRSGFILSGM
                     DTDSQMVSFSNGTQIFQGAEHSQLKEDVQKMQIRRTIGEHLQKEKKLRPLGIKVLSLF
                     FIDKVDNYRPSAQSPTGGKFAKWFEEIYLELAKEEPQGVHNGYFSQDKKGVAKDTNGT
                     TQADNETYQLIMRDKEKLLSLENPLRFIFSHSALKEGWDNPNVFQICTLNDTASEMKK
                     RQEIGRGLRLPVNQQGLRIYDRHLNTLTIIANESYEQFAATLQREIEEDCGVQFAKSN
                     VKKREDKKAVRYRKDFSLHPEFAAIWERIKHQTTYRVDFSNAELIESAVEKIRKMPDI
                     EATKIKHSKVKLEMNETGISTNYRSSNETAVNTQWAIPDLLSEIQKKTGLTRATLCKI
                     LQKSDRLQEVFRNPQRFIDLIAEKINLALQQIMTDGVEYHKIAGKTYEMTLFGDFDFY
                     LNQYTFEVQDKTKTIYEDYIALDSQTENIFARHCESSEQVEFYFKLPKRFKIPTPLGS
                     YNPDWAVVFKGENKIYLVAETKNTGKGIQAGVDEEKLDESERMKIDYARKHFKGLQRD
                     YKDLDYRVVQKVEELSAEYCKI"
     gene            complement(20039..>20660)
                     /locus_tag="PXD81_000568"
     CDS             complement(20039..>20660)
                     /locus_tag="PXD81_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HDL5536630.1"
                     /translation="FPHAILESNRIDKGNRKFIAVQYPEEIDIKSKNGKVAKQFCQRK
                     NLPLYITEISKERIRRAGKQILQNNPENRPLDVGFKVFKLTESHFKQWQSPTSENLAD
                     QLEMFIDPVSEKADTQAVLYELLLRLGLKLTAKVRLENKVFWVEENGLIFALLLNAAD
                     EEIIQTVIAQQPKKVIALDRLFNGNDARKKNTELQMQDAGITFFVI"
BASE COUNT         5712 a         4527 c         3930 g         6491 t
ORIGIN      
        1 acataggtta ttttcattaa tccattccaa gacttttctt aattcttttg aaccaagttt
       61 attaaaaaaa ttctttccta ttttactaaa ctttaattcc ttattctgca aatgaaggag
      121 ttttctccaa tcaggagcaa ttaaattagc attactaatt tgagcaatcc ctcctaatac
      181 aaatacagga ggaaccccaa tgtgggaaga aatggttcta ttaaaattac tacctttttt
      241 atcattaggc gtatgtttta acttcccaat atttccacca ctctcatcaa gaaatatttt
      301 gtagttatgc tgtgctatat ttaatccgtg aaatgatctt tctaagtttg aagttatcac
      361 aacaagtccc tccccaaagt aggtaattat aataaagatc caaacactcc caccacatac
      421 cccttcgatc tatccctttc caacaacttt ggttggcaga ggtcgataag cgagcaggct
      481 ttgcagcgtt tgctgtattc tggaggaggt gtggttccgc tatctaagag cgaccgcact
      541 tcggcaatgg tggcgagggt tttggtgcgt agttcggtgg aaaaaatgat cggcaaacgg
      601 tggcgagttt gcatatacca caacgcccct tcgttaatcg tctgctctgt catttcttcc
      661 aaacataagg cttgggcaca gagttggatt tcatcaatcg gatcaagttt gggcttgcca
      721 cgtttgtatt ccactggttt tagctcgcct gtttttaggt ctcgctcaac caaatccaaa
      781 atgccgctta tgcctaattt ttcggctgca acgtgaaccg ttcgctcaaa gcgaatccct
      841 ttgcgaactt ctggctcacc actatctacc cgctcgtgca acgcttgacc ttgtgcggtg
      901 agatagtttt ctgcccacac ctgttcgttg tggatcagcg cacattgacg tgggcagaag
      961 gcatagtgtt gcaaagcgga aagcgaaatt gaccgcttgt catcgccaaa ttttggctga
     1021 acaagcggta aaatttccgc agaattttgc aaatcgccca ttacagtaat tcttcaacgg
     1081 ttacacccgt taagccttcg gtttgtacgc tgatacagta atcgtcatag tctttcgctg
     1141 gcgtgtcttt ttcaccgttg attcgctcaa cttttaccga gtcaaacagt ttgtgtgcag
     1201 gttggttgcc taattcgctg tcgtgtttaa atacgatcag tttacgagct gccatttcgc
     1261 ctcgtgcggc agaacggtcg tgttcaaaca taagttgcaa ggcttgccaa agtttttgta
     1321 catcttcatc agaaaagccc gttttttcag cgagtttagc tgaaataaag ccgtgaacac
     1381 gataaagtgc gtaaggcacg atatatttac gccccatagt ccgttctttt tcaagatctt
     1441 tttcgttggt cactgccatt cgtgtgatcg atacttctag cggaataatc ggatcgatag
     1501 attgagcgaa cgccaattgc actgggcctc gcacctgtcc gctgtttact tcggtagtca
     1561 ttaccgcccc aaaggtgcga atatcgaaaa agtttttgca catccaagcg gtaatttctc
     1621 gggctttagc ttcctctttt ggtagttttt tcgcttccga ttcaatccct aatgcttcat
     1681 aagcacgttt gttttgtaaa ttcagaacac ttttttcttt aacataaatt tcgtaacccg
     1741 cctcgttttc atagctcatt tcaataaaat tgcggatttt gcgttttagg caaacatcgg
     1801 tgactaaacc tttactggtt tcaggatcaa ggcgtggcat atttcctgca tctggatcac
     1861 catttgggtt accgttggtt acatcaaaga aaaaaacaaa ttcatagcgg ttttgaatag
     1921 acatagtgtt tccttagttt tcaatagact cagtggtttc ttttttagcg aaatagcttt
     1981 gtttttggtg gtaataacca atcgcaaaac gcccttgttc atcgatactt aaatggcgag
     2041 ggaaattttc cggcaagctg gcaataatct cacctaaatc cttatccaga ttaaccgcca
     2101 ttccgccctt atcttttctc aagcgagata aatggtgttt tgcaccgctt aataagcgag
     2161 ggaataccga aaacggcacg gcagaagccg aaccataata gcggtcggca atgccggcat
     2221 ttaattcgcc taatgcttgg ctttggattc tttctaatac tgcaaataac ctgccgagca
     2281 agtaagcttg gttatcgctt tctttattta agctcatagg aactccttct ttaataaaac
     2341 cttttctaaa ttttcgttgt aagactgctt tcatcaacgc tactcgcaaa ccgttaatat
     2401 cgccgtctgc tcggattcga gtcactaact gtgaaagtaa actcatcgga tataaatgac
     2461 cgctaataac tgcgcgagcc atttcacccg ccaagactgg agaaatattc tcacttttac
     2521 ctaatgaagc ggtttgtaat aatagtcgcc aaatggaagg tggtgttttc catgggcaag
     2581 gctcaagagc gagatcttgc caatgctcgc tgaggttttt tgccagttgc ccaaaggtgg
     2641 tatccagcca aaaacgtacc gaaatgcgag ctgcatttgg agcaagcccc aacacataaa
     2701 aacgggtgtt ttcagataat tctggggaaa tttctgcaag cggtcgtcct ttgctgattt
     2761 tttgcaattc ttcaaagatt ttttggtttt cttgctcatc atctggaggc gtcattaaag
     2821 cagcaaaaaa gccttcagca gcttcagctt gggtggtatt gtctgcttct gcccaaaaga
     2881 cagtgctggc atcaccaata gttaaacgat gtttctcggt gcgtaacaaa taatttaaag
     2941 cagtagtgta ggcaaaggcg gcttgttcag aaacaggggc attactacct tgttccttgc
     3001 caaatgaagt gaaggattct ttattgaaag agatgattga accaccggaa ctttgcccac
     3061 caaatacccc tttaatagga ggatgagttc gttgaattgg cgataatttg cctgaaatta
     3121 agcataaacc tttttcagca ttatcacttt gatttatcaa caaacttgtc caaagggttt
     3181 gtgcagcttc tcgctgatga atataaccgc ttgtgccgac taatttaaac acgacattcg
     3241 catcaagaat ttctcttggg cagattgatt cggcaaaatg tgtgggttgc cattgttgca
     3301 ggaaatttct tatcgccatt aaaccttcat cattggaatc tgcaagcaaa tccaaatggt
     3361 actgtttaaa tgcctcaaag gttttctcgg cgaaattgat gggttgatct ttggcggtgg
     3421 ctttatcagg attactttca atgcctagca cataagcggt tttatcccac agaaaattgg
     3481 gcttaatgcc agacgtgcgt ttttcagggc gaggtacgct catcaatttc ggttttggtt
     3541 tcttatcttc tgttaaattt gagatcacat tgaccaaatt tccctttgaa tctaattcta
     3601 gtacccaacc gattttttct tcactgaaac cataagaagg cactttagct tcgcctgtta
     3661 agcgatcttc ttgtgctgca aggcggtaat aatagcgagt taaggcggta ataatagcga
     3721 gttaaggcgg agagaatcat gatttgacct cctctgcata aaatggtggc acatcaatca
     3781 cgccattttt catttccgca cggaagaaac gaggttgatt accgtgctca aaatcaatgt
     3841 cgtgcaacat ccagcctaaa tcccgatctc gctcattttc tgccaaaaaa cactcaggta
     3901 acggttcatc gtcatcaatc aaggcaaaat ttgcggcaaa ttcacgcacg cccatacaag
     3961 gctgctggaa acattgcccc tttttcgcac ggcgtttaaa catttcaata tgcttggtga
     4021 cggtttccgc ctcgcctgcc attttggtca gcactgcgtg agcttcgatc acataaccga
     4081 catttttcag cacagtagca gcacgttgtt ggcggtcgtc ttcaatcaca gtatataaat
     4141 ccgccactgt tttacgcttc attgcaccac tgattttact ggcagaaatt ttactgccta
     4201 actcattacg gcgaacagac tcaaattgaa tcggttgtaa tacataaatt ttatcaatca
     4261 cccagcgaat tgccggcttc caatgcaccg cagccaaaat cccccgtgcc gcagacggcg
     4321 taatcacatc ataagacacc cgttctacct tcatttcagg acgggtaaaa caagcataat
     4381 caccccaaat atggagcctg attttgtttg gcataataga atgctccttt taattatgta
     4441 acacacacct ccccgaaggg aggtgaaatt tctttagctt atattataac tgctttggtt
     4501 tcaacccacg cactcagtaa aatgcgaaaa attttatggc ttattatatt aagcggtcgg
     4561 atttacttag aaagttgcaa agttatttga gtatttaacc gcttgtattt caaaaatagt
     4621 cagaaaaata acagcattct aggtacaata tttttctaag aacgaacttg tctccgcaat
     4681 gcctctactg cgtttgacac agcatttggt ttgggctcct ggtttgccca atcagaccag
     4741 aattggcgtg tagcactttc tggggctata tgcggtttag caagtttgat tcgtgtaggt
     4801 aatagaatcg aatcgcctag caataacgct tcgcctaaat caagtaacgg caatatatca
     4861 gtaattccaa ccatagattc cggcaataaa cgctttacaa cactttgatc ttgaccattt
     4921 gttaatcgta aaaccaagaa attattacat tgactcaaaa ttgttcggct tacatctgaa
     4981 ggacgttggc taacgaccaa caaagacacg ccatattttc taccttcttt cgcaatccgc
     5041 tcaaaggctt ccaatgcgcg tttttccgct gcttccgctg aatctcgaac agggagatat
     5101 aaatgtgctt catcgcaaat aatcgttagt ggatggcggg aatctttatc catccaaaat
     5161 tgaatgttgt ataaaaggcg ggcaagaact cccgtaacaa tcggtaaaac atccgacggg
     5221 acttccgaaa aatcaataat ttttatccct ttttcgctct cagaagcaga aaacagtttt
     5281 gacaacatat tttccagcca cccatagctg ttgcactcta ctggcggttg gaacataaaa
     5341 ccgcaacgtc tgtcatctat tttggctttc atccttccca taaacctaga caacttacta
     5401 taccaaggac cttgctttgt tcctgaacct ccctctttag gtaccatctc agaattatct
     5461 ttatctaaac gagctaacaa taattcaata gaatacggaa ttggcgaatc aacagtaaac
     5521 gtttccaaaa catcatttct ttcttcctta ttcaatgttt gctcttttaa tgcgcgaaca
     5581 tgctcagtaa aacgcgccgc ctgattcgga gcattatcat cactgcgatc taaaattaaa
     5641 gccagcattt attcccgatt gagcagccaa tagggaataa ataaactgcg ttcatctgaa
     5701 ccatccaaat ctcccggtcc agcaatacgg tattgctgag cgatagcgtt tttaccttcg
     5761 catagagtgc tatattcacc gtgcatatcg aatacaataa gattagcagc ttttaaagct
     5821 gctgcacgtt ccaatattaa tgcaactgac cagcttttac cggaaccagt actacccaaa
     5881 atagcagcgt gccgttgaaa gaatttatct ccgtttgcta tagctacagc cgtttcatca
     5941 gccataaaat gacctaaagt cagccgttca ttttctttta cgttagcgga caaaagattc
     6001 ataaataatt gcagattgct gccactaata tgaaagcaat ctctatcaat ttgcggaaaa
     6061 caatccgctc cccgcttaaa agtgttgctc aaactaccat caacagcttt ataagtaccg
     6121 attaatactg ccttgataaa gtcatcctga ctttcaccca gaggaatttc accctcatta
     6181 gatatttctt ctagcagcaa tttttcactg atagaacgcg ttacccgttc gataatgccg
     6241 ataaggtact cactagccgt tctgccttga atcgcaatca gattacccac agcaatacta
     6301 gtcatttgtt gatgctcttc aacagaaatc aatacacggc tggtatctac tgttacaacc
     6361 ctgcccaaac gggcattgtc tttaaaattc aatgtttcat ttgccattct ctaaaatctc
     6421 ttttacaaaa aactcaacgt cccaccaatg aacattagga ataaaagttt cttcttgctt
     6481 gctaaccacc cttgttccag ccttatctcc atcaaaaatc agagccttac aattcggagc
     6541 ttccttcacc acgtttctgg cattttctga aagtcctctt gtcagaagta gtgttttcac
     6601 accactttta attttgcctg tcaaatgtgt ttgaaggtgg tcatcattaa aaccatagcc
     6661 gatacataaa attgaagaag cagaatcaat agcttcattt gctttttctc gatgagcatc
     6721 aaatggttgt ccatagcctt ttttatactt cccaacacca ggcgtaataa ttaaaggctc
     6781 ttccacttta cctatacaac ttgtcattgg aacgtctcca gccataaacc aatctaaact
     6841 accatgcggt ttaaagattt tgatatgatc tcgttgacat cctcccctgc ctaaaggcag
     6901 gggattccta ctagctccca cagcaagctg tgggctactc tcggtgggtt tctactgctg
     6961 atctctattg agattcactt catagacgct tcgggtatgt cctaccctga tatttattat
     7021 gcttggacta ttgcttgccc acgtgtaaga acattcattg ctcccactac atcggcattt
     7081 tccgtatagc cacattctac gcattcaaaa ttttcctgtg tttggcgatt ttcttttgct
     7141 gtatagttgc aacaagggca agtacgactg gtattttgtg gaggaactgc tagcaaaaat
     7201 ccgcctaacc attgcgtttt gtagtccagt tgtcgtctaa attcaaacca agactggtct
     7261 aatatcactc ggtttaagcc tgatttctgt ttcacatttt tgccgtgtac ttccgctgtg
     7321 cctttggctg atttggacat attcgccact tgcaaatctt caacatagat catcgcgtgg
     7381 tttttgctga tttgcgttga gattttatgc aggaaatctt ttcggcagtt ggcgattttg
     7441 tgatgtaact tgccgatttt atctttcagt ttttgccagt ttttactgaa cttcacttta
     7501 ttttttagct gtttttgcag ttttgccaat tttcctttgt gggttttaaa cgcatttaac
     7561 ggctcaaaaa attctccatt ggaaagggtt gcaaaacgag caatgcccat atcaatgccg
     7621 atttctccgc ctttatgcgt gggaatttcc tgtttaaatt ccgtttgaat gctcacaaaa
     7681 aagcgtccgc atttcattga aacggtgaca ttttttactt ccccaacaat ttcacgactg
     7741 ttgcgataac gcacccaacc gattttcggc agatacaagc ggtcgttttt ctgctcgatt
     7801 ttgcaacctt gcggaaaacg gaaactctct tttacgcctt ttttcttgaa tttgggcaaa
     7861 tccgcacgct tttgaaagaa gtttttaaac gcactttcaa ggtcttttaa cgactgttga
     7921 agcacttgtg aatggcaatc tttaagccat accaattctt ttttccattg cggaagcaag
     7981 ttggcgagct tggtataact gaatttgtga tggttatctt gttcgtactg ctcattttgc
     8041 cacgccaatg ccttgttaaa cacaaagcga gaacagccgc aaaactgctt cattttgcga
     8101 acttgttccc catttggtct gatttcaaat ttaaaggctt tacgtagtaa cattgcacga
     8161 attagaatac aaaaattgtg cttattctac acttggccta tgaaaaaaga aacagagatt
     8221 agacgtggtc gccacgttgt ttttaactta cacgttcatt tagttttcgt gactaaatat
     8281 cgcagggaag tttttactca agcaattttg aacgacttac actcaatttt tgcaacgatt
     8341 tgtgctgatt ttgaagccac attagtggaa tttgatggtg aagatgatca cgttcatcta
     8401 cttgtggaat atccgcctaa agtcgctatt tctcatcttg taaacagttt aaaaggcgtt
     8461 tcgagccgaa tgattagaaa gaaaaactat ccgagcattc gcaaaaaatt atggggaaat
     8521 caactttggt caccgtctta ctttgctggt agttgtggtg gtgcaccaat atcgattatt
     8581 cgccaatata tcgagcaaca acaaacacct gattagttag aaatttgaaa cggctttgcc
     8641 gtttatacct tatatccacg ccctaaagga cgtggtttta cggcacgatt agataaacca
     8701 attttggaca tttacctttt actctaatgt ctcttatctg caatttccta ctcaaatcag
     8761 gattatgttt tccccaataa cgaccgacca tcattgaatc tattccccaa ttttcatact
     8821 ctgccgcaaa ctcaatcaat ctatcataat tagtagtaat tacacgggca attttaggat
     8881 ttgaagctga aatatgggga agcaaataag aaaatttgag cttctgattc tcagcaatac
     8941 atttgtttat cgctttttgc tcttcggaga gtacatactc ggctgttatt ttaataatag
     9001 cggcttctat cgtctcattt gcttgatgct tcaacaatgc accttccaag ccttcagaat
     9061 ccagacaatc agaaatggta ttccatgtta ccttatctat atcatcaaga cattcaggca
     9121 ttcgttcttt taatctatca gctaatgctc ccattcccgg caaaccttcg gcacaggaca
     9181 aacccgaacc gacaataatg accaaattgt cagtaaaaat agtttgcaac cattttttta
     9241 attcatcttc atctaataat ggttctctca ttttacgttt cccttccaaa tatctattct
     9301 tacaaactag ctgtttacta cgagacgtca taggctattt ggtatattaa agtatatcta
     9361 tgcaaaatta aacttataaa gaagttaaaa taaacattca tctataaaac caatttctcc
     9421 gactcaagaa aatccacatt ctcccaactc aaccccgcca cttcatcata caaatccatt
     9481 ccaattaagc agtaaaactg tttgccgaag ttggtttcat taatggtttc aactctgcca
     9541 gcgttataga gttcggctaa tgctttttcc ggtacttgca cagtgtaagg ctggagtttt
     9601 cttaaaatac cgcctacttg atccgcatag cgtaaacggt caataaggtt ttcggcttct
     9661 tggtcgaaag ggataatcac cggaattaaa tggcttgtga tcattcggaa atcttcagct
     9721 attttttgga atgggaaatt aaggctttgc ccagcgtcgt gacacgcctt taaaatattt
     9781 ggcttatcca gctcttttcc tttagcctca taaaccgctt ggaaataatg attgattgct
     9841 ttcggattaa gtagatcatc tgcgtgaacc tgaaccgttg aacgcataca gccagaaagt
     9901 aagcctagct ctggtggtgc tttccactgt tcttcaggtt ggaaaaccca aacattgctt
     9961 tctacagata aacgtttccc ctctcggttg caacgccctg ctgcttgagc cactgaatct
    10021 agtcctgctt cagctcgcat aaccagtggg aaatcgacat caacaccggc ttcaattaaa
    10081 gaagtggcaa tcactcggca aggtaggttt tgttttaggc gaagacgaat ttcagtcagc
    10141 gtttgtgagc ggtgtttagc acacattagc gtggtgaggt gataagctcc ctctaactgt
    10201 ttcgctgaat catataacgc tcgggcgtga cggcggttat tcacgattat cagcatttgt
    10261 gggttatctg cgagtttgct ttgcaactcc acatctgttt gaataccgat atgctttacc
    10321 gtagtacgtt tcaatttttc aaaaagtgcg gttggattag gggcaatttc tcgcacattt
    10381 tcaaatcctt tataaaaacc gttcgcttga tgaatagcgg gttgtgtcgc tgtgcaaagc
    10441 actaccgaac attgataatt gcgagctaat tcatcaatcg cagccataat tgggcgtaat
    10501 aaattcaacg gtagcatttg ggcttcgtcc aaaataatca cgctaccgac gatattatgc
    10561 aatttgcgac agcgtgaaga acggtcggca aacaaacttt caaagaattg cactgcagtt
    10621 gtaaccacaa caggagtatc ccaattttct gatgccaatt tcagcttatc tcggcttgat
    10681 ttatcaggta ggctatcgtg atcaaaagtg ctgtgatgtt ccaataccgc ctctgttcct
    10741 aattcgccaa aagctttacg aaattccgca gcattttgtt caataatgct ggtaaacgga
    10801 ataacataaa taatgcggca taaaccatac tgtttagcat gctcaagggc aaacgccatt
    10861 gaagtaaagg ttttaccacc gcccgttggc acagtcagag aaaacagacc tgtttcctgt
    10921 tttgcttgag atacggcata atccaaaatt tcactccgca agcggtttac ttccgcttta
    10981 cgttgtgctt cctgcgtatt aggcgtttga gtggcaattc gctccctaaa ctgtgcaata
    11041 aattgattaa attgctgatg gagtgcggtt aaattggggt aatttcctcg ttcaagcggt
    11101 cttttttcga gattggcata aaaagcttct gtatcaagaa agtcggcatc gacaaggcaa
    11161 gaatagagca ttcggataaa aaaggcgtaa gaaaagaaag gttcgttttt agaaggcttt
    11221 aacggcggta agggcaattt ttcaggcaaa ttaagttctt gctgccaaat agcgtcaagt
    11281 accggtagat ctttatgacc atagccaaat tgctcttgca aacgttgtgt aagtgtagaa
    11341 cgatttttcc cttctccaga accatttgct aacccagcgt ggtggcctgc gatacaaaac
    11401 gccatcattt tgcccaaagg cccccaacgt tccactgcaa tttttgcacc agcagtagag
    11461 tgatttaccc gttctccgcc atgtaaacgc ttttggtaac gttcactata tttgcctaaa
    11521 tcgtgcaata gccccgtgca gcgagcaatt tcttgtgaag caaacggcgc agcaaattcg
    11581 gcggcaagca agccaacatt attggcgtgg ctgagtaaag tttgccaatc tgaatgatct
    11641 gaaagcttcc ccgaatgggc ataaaaagac tgaatagtat gttcttccat ttttatacct
    11701 tcaatatttc cttcctaaca caagcaatac tacctctaac cttttattct gtatatatcc
    11761 tagataatat tttcttaatc taaatcaaac acaaatttca cttgcaaaat tcaagtaaaa
    11821 tttgaccgct tgtaatcatt ttttctaagt tttgaaatct gttctttcag cagaaatttc
    11881 acaaaaaatt tacttatcta tggggatttt tgctagaatg ttgccatttt tacgacaagt
    11941 taaaggtatt atcatgtcag aacaaacttt tattaccggt aaagatgctg cacttgaaga
    12001 cagcattgcg acctttcagc ataaattaaa aaccttaggt tttaatattg aagaagcctc
    12061 ttggttaaat cctgttccga atgtgtggtc ggtgcatatt cgtgatgcag actgtccgca
    12121 atgtttttct aacggtaagg gagcaagcaa aaaagccgct cttgcctctg cattaggtga
    12181 gtattttgag cgtttatcta ccaactattt ctgggcggat ttttatttag gtcaagagat
    12241 tgctaatagt gaatttgtgc attatccaac cgaaaaatgg tttccgattg atgatgaagc
    12301 cattccacca atggagatga tggacgagtt tttatgggaa tattttgatc cgaatggtga
    12361 aatcacgccg gaattattgg ttgatttaca atcaggaaat tatgatcgtg gaattgttac
    12421 cctaccttac gttcgccaat ctgacaatga aacggtttac attccgcaaa gcattatcgg
    12481 caacctgttt gtttcaaacg gtatgtcggc aggcaacaca aaaaatgaag ctcgagtgca
    12541 gggcttatcc gaagtgttcg agcgttttgt caaaaaccgt attattactg aggcaatcag
    12601 cttgccggaa attccgcaaa gtgtgattga cggttatccg acaatcaaag cctctatcga
    12661 gaaactagag caagaagact tcccgatttt atgctatgac gcttcattag gcggagaatt
    12721 tccagttatt tgtgtgatct tgttaaaccc aaataacggt acttgttttg cctcttttgg
    12781 tgcacacccg aatttccaag tggcgtttga acgtaccgta accgagttat tacaaggccg
    12841 tagcttaaag gatttagatg tgtttgcccc accttcattt aataatgaag atgtagcaga
    12901 tcatgcgaac cttgagacac actttattga ttcaagcggt ctaatttcgt gggatctttt
    12961 caaacgtgat gcggattatc cgttcgtgca ttgggacttc tccggcacaa cggagcaaga
    13021 atttaataat atgatggcga ttttcaacaa gcataatcag cctgtgtata ttatggatta
    13081 tgagcattta ggtgtgtacg cttgccgtat tcttgcaccg ggtatgtcga atatctatcc
    13141 aagcgatgat ttggtctatg ccaataacaa tatgggtatg gattggcgtg aaatcttgtt
    13201 agatttgcca catttccatc acgatgctga aacttaccaa gaactgcttg atgaattaga
    13261 tgagcaaagc attgatgatt taacccgtgt gcgtgaattt attggcgtgg tggcagagaa
    13321 aggctctgca atgcaaacat tgcgtatcgg tgagcttaaa tcgatgctgc atttagcttt
    13381 aggtaactta gagcaagcct cagactgggc aaactggaca gcgaatatga atgcaagcgt
    13441 attctcagca gaacgtaaca attattaccg ctgcttaatc caatcgattg agttgttctt
    13501 agatgaagag cgtgattcgg aacaatatcg tatggtgttt gagaaaatgt acggtaaaca
    13561 agcggttgaa gctgcttggg gtgcaatcca aggcggtaac ccgttccacg gtttaactgc
    13621 atcggacgaa tcgcttgaaa atatgactgc acatcagaaa ttactgaacg cttatcgcaa
    13681 agtgcaaaaa gcgaaagtaa atccgattgc gagataattt tatatttttc gtaggggtag
    13741 gttctgtgcc tgtcctttct atttactacc tttcgggagg gcataggtct ctcccctaga
    13801 cataaatttc aaaatgattg actttcgtcc tttttatcaa caaattgcca caaccgacct
    13861 ttctgcttgg ttagaaacct tacccttaca actcaaacaa tgggaaaaga ccacccacgc
    13921 tgactatgcc aagtgggcaa aagttgcaga ttttttacca aattcgaccg cttgtatcaa
    13981 tctaaaagac aaggttgagt ctatttcaag caagccttta tctgaggggg aagtcaagca
    14041 aattacccac catttaaagc aactaatgcc gtggcgtaaa ggcccttatc atctacacgg
    14101 gattcatatt gataccgaat ggcgttccga ttttaaatgg gatcgtgtgc ttccgcatct
    14161 tgcaccgtta aaagaccgca ccattttaga tgtcggctgc ggtagcggtt atcatatgtg
    14221 gcgaatggta ggcgaagggg caaaaatggt agttggtatt gacccgaccg agctgttcct
    14281 gtgccaattt gaggcagtgc ggaaactctt agggaatgat agacgggcta atctgattcc
    14341 acttggtatt gagcaaatgc aaccgcttgc tgcttttgat accgtgtttt caatgggggt
    14401 gctttatcat cgcaaatcac cgcttgatca tctttctcaa ctcaaagccc aattagtcag
    14461 aggcggtgaa ttggtgttag aaacgctggt gattgatggc gatattaatg atgttttagt
    14521 gccggctgat cgctatgcga aaatgaaaaa tatctatttt attccttctg ttgctgcact
    14581 cattaattgg ctggaaaaag tcggctttaa aaatgtgcgt tgtgttgatg aggcaatcac
    14641 tacactagaa gagcagcgaa aaaccgattg gttggaaaat gaaagtttag tggatttttt
    14701 agatccaaac gatcatagca aaaccattga aggctaccct gctccgaagc gggcggtgat
    14761 tttagcgaat gcttaaaatg ctagtttaag agaataaaaa atccgcatgt caaatgcgga
    14821 ttttttaact aaatatctaa ctgagcatac aaagcgttag tttcaatgaa atctcggcgt
    14881 ggttcaactt catcgcccat cagcgtactg aagagtttat ctgccgcaat cgcatcggta
    14941 atattgacct gtagcatttt acgagccacc ggatccatcg tggtttccca taattgttcc
    15001 ggattcatct cacctaaccc tttataacgt tgaatagtta agcctttacg agattctttc
    15061 accaaccaat ctaaggcttc agcaaaactg ctgatttctt ggcgacgttc accacgcacc
    15121 acataggctg aatcagatag taaatcgttc agctcgttgc ctaatgccac aatgcgagcg
    15181 tattcattac cgttgataaa gttgtaatct aaacgatagg tactgtccat accgtgtgta
    15241 gtcacaataa tttccggctc ataaatttgg cgttcttcat tgaacaaggt attaatacgg
    15301 tagaaactgc cgcccacctc ttttgccatc aatgtagcga caaattgctc gctccaagcg
    15361 gtcatatttg cttgattttt tgcaaattcc accgaaagcg gttttgaata aattaattcg
    15421 ttaagtaata atgttgggta acgacggctt aaacgttcaa ttaatttttg tacgccgtta
    15481 tattgggcaa ttaagttctc taacgctacg ccacttaacg ctggagcatc ttcactcaca
    15541 tataagcctg caccttctaa tgcaatatca atttcatatt gcgacatttc atcgttatct
    15601 tgaatataac gctcttgctt gccttttttc actttataaa gcggtggctg tgcaatataa
    15661 atatagccgt tttcaatcag ctccggcatt tgacggtaga agaaagttaa taaaagcgta
    15721 cgaatatgtg caccatccac gtccgcatcg gtcataataa tgattttgtg gtaacgtaat
    15781 ttttccaaat tataatcatc acgcccgatg cctgtgccta aagcggtaat caaggttgct
    15841 acttcttgtg aagaaagcat tttatcaaag cgggcttttt ccacgttcag aattttccct
    15901 tttaacggca aaatcgcttg gtttttacgg tcacgccctt gttttgccga gccaccggca
    15961 gagtcaccct ccaccaagta aagctccgat aacgccggat ctttttcctg acaatcagct
    16021 aatttacccg gtaagccgcc taaatctaac gcccctttac ggcgggtcat ttcacgggct
    16081 ttacgagctg cttctcttgc acgggctgca tcaataattt tggtaacgat attttttgca
    16141 tcgtccggat tttcggctaa atagtcggat aaagcctcat tcattgagct ttccactgct
    16201 ccacgcactt ctgacgacac caatttatct ttggtttgtg aggagaattt cggatccggc
    16261 actttcaccg aaatcaccgc tactaaacct tcacgggcat catcacctga ggcatcaatg
    16321 ttcgcatcgg cttttttcaa taccccgctg ttatccatat agtttttcaa cgcacgagtt
    16381 aatgccccac ggaaaccggc taagtgcgta ccgccatcac gctgtggaat attattggta
    16441 aagcagtaca cattttcgtt gtaactgtca ttccactgta gggaaatttc cacgccaatg
    16501 ccgtctttct cagtggtcaa atagaatggc gtattgtgaa tatgagtttt gccttcgttt
    16561 aaatactcaa cataggcttt aataccgccc tcatatttaa aatgctcttc tttgccgtct
    16621 cgttcatcaa ttaatttaat cgaaacgcct gaattcaaaa atgatagttc acgcaagcgt
    16681 ttagacagaa ttttatattc aaattcggtt ttatttttga aaatatccaa acttggccag
    16741 aaacgaaccg acgtccccgt tttttcggta ttgccgataa tcgctaaagg tgcatcaggc
    16801 tcacctaagc tatagaattg ctcgtgaacg tgtccttcac ggcgaatagt gagttgtaat
    16861 ttagaggaaa gtgcattcac caccgatacg cccacaccgt gcaaaccgcc ggaaacctta
    16921 taggagttat cgtcaaattt accgcctgcg tgaagaacag tcataatgac ttctgctgcc
    16981 gaaacgcctt cttccgggtg aatccccacc ggaattccac gaccgtcatc ttgcacagaa
    17041 accgaattat cggaatgaat tgtgacaata atgtctttac aatgcccggc taatgcttcg
    17101 tcaatcgcat tatcaaccac ctcaaacacc atatggtgta agcccgtacc gtcatcggta
    17161 tcgccgatat acattcccgg gcgtttacgc accgcatcta agccgcgtaa caccttaata
    17221 ctggaagaat cataacttgt tgcttgagtt tgttcagaca ttcttattcc tactaatttg
    17281 aaatcgaaaa aattgtgccg attatagcag aaaaatgcgt aaaagtatcg caaaacaagc
    17341 ggttaaattt tagttgaaat tgcagtctat ctcaattcta caagaccaca gattaaattt
    17401 tacaatactc cgcagacagc tcctccactt tctgtacaac cctataatcc aaatccttat
    17461 aatctctctg taagcctttg aaatgcttac gggcatagtc gattttcatt cgttcacttt
    17521 catctaattt ttcttcatct actcctgctt gaataccttt gcctgtattt ttcgtttcgg
    17581 caaccaagta aattttattt tctcccttaa acacaactgc ccaatcggga ttatagctgc
    17641 ctaatggcgt tggaatttta aaacgtttgg gtaatttaaa ataaaactcc acttgctcac
    17701 tagattcaca atgtcgggcg aagatatttt ctgtttggga atccagtgca atatagtctt
    17761 cataaatcgt ttttgtttta tcttgaactt caaaagtata ttgattgagg taaaaatcaa
    17821 aatcgccgaa taacgtcatt tcataggttt ttccggcaat tttgtgatat tccaccccgt
    17881 ctgtcataat ttgttgtaaa gcaaggttga ttttctctgc aattaaatca ataaagcgtt
    17941 gaggattgcg gaagacttct tgcaagcggt cggatttttg taaaattttg caaagggtcg
    18001 cacgcgtcag cccggttttc ttttgaatct cactcaataa atcaggtatc gcccattgtg
    18061 tatttaccgc tgtttcatta gatgagcgat aattagttga aatccctgtt tcattcattt
    18121 ccaatttcac tttggaatgc ttaatttttg tcgcctcgat atcgggcatt ttgcggattt
    18181 tttccaccgc actttcaatc aattccgcat tactgaaatc tacccgataa gtggtttggt
    18241 gtttaatccg ttcccaaatt gccgcaaatt ccggatgtaa gctgaaatct ttacggtagc
    18301 gaacggcttt tttatcttcc cgttttttaa cattgctttt ggcaaactgt acaccacaat
    18361 cttcctcaat ttcacgctgt aatgtggcgg caaattgttc gtaactttca tttgcgataa
    18421 ttgtcagcgt atttagatga cgatcataaa tccgcaatcc ttgttgattc acaggcaaac
    18481 gcaagccccg cccaatctct tggcgttttt tcatttcgct tgcagtatcg tttaaagtac
    18541 aaatttgaaa cacattcgga ttatcccatc cttcttttaa ggcagaatgc gagaaaataa
    18601 accgtaacgg attctccagc gaaagcaatt tttctttatc ccgcataatc agttgataag
    18661 tctcgttatc cgcttgcgtt gtgccattag tatcttttgc tacgcctttt ttatcctgtg
    18721 agaaataacc gttatgcacg ccttgaggct cttctttggc tagctccaaa taaatttcct
    18781 caaaccattt tgcaaatttt ccccccgtag ggctttgtgc agaagggcga taattatcca
    18841 ctttatcgat aaagaacaaa gacaacacct tgatcccaag cggtcgtaat ttcttttctt
    18901 tttgcaaatg ctcgccaata gtgcggcgaa tctgcatttt ttgcacatct tctttaagtt
    18961 gggaatgttc cgccccttga aaaatttgtg tgccgttgga gaagctaacc atttggctat
    19021 cggtatccat tccgcttaat ataaaccctg aacgatacat ttcattttgg ttggaaagct
    19081 catacaaatc ctgattcggt ttcacgctaa tcactttctt tttcatcgct tttttatcgt
    19141 taaataaaat ttcgattttt gccgaccagc tttttgcccc tgttttaatc tctttgaggg
    19201 aaagataagc tccatttacc tcattttccg ccaatacgct atctacctca atttgtttaa
    19261 ctaatcccat ttcataggct tgcaccggat tgaggctaaa aatcgggtta tacgcatttt
    19321 tatgggtggc agaataacgc aaagtgaaaa gcggacttaa actctcaatc gcattacggc
    19381 gaatctccgt ttccatattt tgcggttcat caataatcac aatcggattc gcctgttgaa
    19441 tatagaaaat cggagcatca ccgctttcat tcacggtatt aatcacgtta ctttctttgg
    19501 tgaaagcatc aatattcatc actaaaattt caatatgatt agcactagca aaggatttta
    19561 agcgtgaagt ttggttactt ttatattcaa atttttgatt gacgctcggg ttatcaaata
    19621 ccgtattaaa atggcttttt gtagtttcca acgtatgcaa cactccctca cgaattgcca
    19681 cactcggcac gacaatcaca aatttttgcc agccgtattg acggtttaat tcaaaaatcg
    19741 tgcgtaaata gacataggtt ttccctgtac cagtttccat ttccacggta aaattttttc
    19801 cctgttcgac aagcaaagtg cggtcgattt tttgcctgtt ttgcagatta gccaaattct
    19861 gttgtaactg ctcatcacta atctgcaagc ctaaattcgg cacaaaccgc atatcatttt
    19921 gggctttgag tgaaaattca gttgcggcat tcggctgtcc gacaaacaaa tttactgtgg
    19981 cattaacggc ttgtaattga tactctaagg tttcaaattg gagttccatt ttttctcctt
    20041 aaatcacaaa aaacgtaatg ccggcatctt gcatttgcaa ttcggtgttt ttcttccggg
    20101 catcattgcc gttgaataaa cggtctaaag cgatgacttt tttcggttgt tgagcgataa
    20161 cagtttgaat aatttcttcg tcggctgcat ttaacagcaa ggcgaaaatc agcccgtttt
    20221 cttccaccca aaacacctta ttttccaacc gcacttttgc cgtgagtttt aaccctaaac
    20281 gcaataaaag ctcatataaa acggcttgcg tatccgcttt ttcactaaca ggatcaataa
    20341 acatttcaag ttgatcggct aaattttcag atgtgggcga ttgccattgt ttaaaatggc
    20401 tttctgtcag tttgaagact ttaaaaccta catcaagcgg tcggttttcg gggttatttt
    20461 gcaaaatctg ctttccggca cggcgaatac gttcttttga aatttcagtt atatatagtg
    20521 gtaaattttt tctttggcaa aattgctttg ctacttttcc attcttactc ttgatatcaa
    20581 tttcttcagg atattgaacc gcaataaatt ttctattacc cttatcaatt ctattacttt
    20641 ccaatattgc gtgtggaaat
//
LOCUS       DANLYI010000020        20633 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.020, whole genome shotgun sequence.
ACCESSION   DANLYI010000020 DANLYI010000000
VERSION     DANLYI010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 20633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20633
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.020"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            <1..660
                     /locus_tag="PXD81_000569"
     CDS             <1..660
                     /locus_tag="PXD81_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease"
                     /protein_id="HDL5536631.1"
                     /translation="SQIFSFFDKKEPSKAQEKSTQSSQSHQVSTSSLGNYDVQMKNDR
                     LKQHIVDTDYYTLALSWSPAFCQKQKRNNHGKIPSHLEYQCGGSADFGWVIHGLWPQS
                     GKARSVDEHPRYCQGDLPMVAEQTIKQYLPESPGATLLQGEWEKHGACAFNQADDYFA
                     KQKELFNQLKLPDTEMSKLALFNWMRANNPHLQNVYLGGSKDELYICYDKAFQPMDCP
                     R"
     gene            660..1154
                     /locus_tag="PXD81_000570"
                     /pseudo
     CDS             660..1154
                     /locus_tag="PXD81_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249134.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
     gene            1259..2539
                     /locus_tag="PXD81_000571"
     CDS             1259..2539
                     /locus_tag="PXD81_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005693148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDL5536632.1"
                     /translation="METIVIVGGGAGGLELATYLGNKLGRKQKAKVVLIDRNATHLWK
                     PLLHEVATGTIDEGTDALSYRAHAKNHSFEFQQGTLVAVDREKKTVSLAPIYNDQNQL
                     LVKERHISYDKLVIAIGSKSNDFGTKGVAENCIFLDGSEQAKDFQKRMMELFLRFSHS
                     ENKDVKIAIVGGGATGIELSAELYHVVKNLNSYGFGKLNRASLKVTLIEAGPRLIPAL
                     PEKVSVSAFHELKKAGVSVRLGTMITEARPDGLMTRLGEKIEADIMVWAAGVKAPEIT
                     REFGFETNRLNQIEIKDTLQTTVDESVFVIGDCASLIQDGKPIPPRAQAAHQMATRCG
                     KNIVALIEGKEMKPFRFNDKGSLLSFSKFGTVGNLMGNLVSGDMFIEGKIARLAYLSL
                     YRMHQVALHGCVKTGLIVLVGQINRFLRPTMKLH"
     gene            complement(2626..3663)
                     /locus_tag="PXD81_000572"
     CDS             complement(2626..3663)
                     /locus_tag="PXD81_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL5536633.1"
                     /translation="MNVLKLISLKSILIASGIFLSAQAAQAEGRLTVYCSVQNNVCEE
                     MTKQFSHKYNVETKFIHGGTGTIFGKVKAEASNPQADIWYGGTIEPHFQAGELGLLEA
                     YRSPKQAEILPQFKSLVESEQGKFTSIVYMLVLGLGVNTEKLAKLGIEAPKKWEDLSD
                     PRLKGEVQLPDPRSSGTTYTFMTTLSTLWGEEKTFDYLKKLNNNVSQYVKSTLVTSNL
                     ARGESAVTVGFVHSYATEKEKGAPIEAIIPEGKVGYALGGVSIIKNARNLDNAKLFMD
                     WVLSKEAQEIPWKKHGVYQTPTNINAEVAPQSTKAEALDFVEIDYQKAGSSEEGKRLI
                     DKWLAEIKLAN"
     gene            3885..4579
                     /locus_tag="PXD81_000573"
                     /pseudo
     CDS             3885..4579
                     /locus_tag="PXD81_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249137.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
     gene            4590..4892
                     /locus_tag="PXD81_000574"
     CDS             4590..4892
                     /locus_tag="PXD81_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HDL5536634.1"
                     /translation="MFAALVLGSQICRFLPEFLPKKVLSSPILQKLNKMLPLVIMLLL
                     LLTSLNFPKNGEGFNLILAQILALIGVLGSYVWLKNTFLSVALGIFGVNVFLSLLG"
     gene            complement(5013..6314)
                     /locus_tag="PXD81_000575"
     CDS             complement(5013..6314)
                     /locus_tag="PXD81_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HDL5536635.1"
                     /translation="MSLFKFEERGSNARQEIIAGLTTFLAMVYSVIVVPGMLSQAGFP
                     AESVFIATCLVSGLGSILIGLWANAPMAIGCAISLTAFTAFSLVLGQQVSIPVALGAI
                     FLMGVVFTLISATGIRTWILRNLPSSIAHGAGIGIGLFLLIIAATNVKLVVNSGMDIP
                     VKLGDFTSFPVLVSLIGLAAIIGLEKLKVKGSILWVIIAITIVGLIFDPNVKYTGFFK
                     MPSFGENSQFLDLDIMGALNTAILPVVFALVMTAIFDATGTIRAVAGQAGLLDKDGQI
                     INGDKALTSDSFGSILSGLFGTAPAAVYIESAAGTAVGGKTGLTAVVVGIGFLFMLFF
                     QPLAFLVPSYATAPALMYVGLLMLSNVSKLDFSDFVGAMSGLVCAVFIVLTANIVTGI
                     MLGFATLVIGRLISGEANKLNLGTVIIAIILVAFYAFDLAI"
     gene            complement(6447..7421)
                     /gene="secF"
                     /locus_tag="PXD81_000576"
     CDS             complement(6447..7421)
                     /gene="secF"
                     /locus_tag="PXD81_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HDL5536636.1"
                     /translation="MAIENVQQDVAEIKLPYKLIPFMKYRYVGFIFSLIVTALCIFTI
                     ATKGFNWGLDFTGGTVIETQFSQPADLAKLRTELENSGYGNALVQTTGSQKDLMIRLA
                     ASAGDMSVGNKVMEMIHQKLDPNATIKSIEFVGPNVGEELTQGAIYATLATLAMLLIY
                     VGTRFEWRLAVGGIVALFHDVLVTIGIFSFLQIEIDLTFVAAILSVVGYSLNDSIVVF
                     DRVRENFSKVRRSSSIEIIDISLSQTLSRTLMTSVTTLFVVLALFYLGGPTLHSFSLA
                     LLIGIGFGTYSSIYIAIGVALQLGLKREHMVQPKVEKEGADQDAFIDY"
     gene            complement(7431..9272)
                     /gene="secD"
                     /locus_tag="PXD81_000577"
     CDS             complement(7431..9272)
                     /gene="secD"
                     /locus_tag="PXD81_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HDL5536637.1"
                     /translation="MLNRFPLWKNLMVIFVIAIGALYALPNLYGDDPSVQISGTRGQQ
                     ATPETLSQVQSTLSAMNIQPKSAVLENGSILVRLESSEQQLPAKEKISEALGNNYSVA
                     LNLAPATPQWLSDIGGSPMKRGLDLLGGVRFLMEVDMNTALAKQQDTLQDSLRSDFRK
                     EKLQYQAVKKGENFATEVEFADNDTAEKAVRLVRLSHPTLDILVNDNKVTLTPSAQAL
                     SESRTMAIEQNLSILRKRVEELGVSEPTIQRQGADRIVVELPGVQDTARAKELLGATA
                     TLEFRLVNTEANVASAAKGIVPADSGLQNTRDGEPVVLRRKPSLGGEHIIDATSGTDE
                     RGLPQVSIRLDTEGGEMMGEITKMAIQKPMATLYTEFKDSGRKDANGKVILEKHQEVI
                     NVATIQTRLGNQFQITGINSPAEAQNLAVLLRSGALIAPIVIVEERTIGASLGADNVK
                     QGMEASILGLGLTILFCLAFYKVFGIFASLALIVNMILTIGLMSLIGATLTMPGIAGI
                     VLAVGMSVDANVLIYERIKEEIRNGRPIQQAIHEGYNGAFSSIFDSNLTTILTSLVLY
                     GVGTGPVKGFAVTLALGVIISMFTAITGTRMLVNWVYGGKRVKKLWI"
     gene            complement(9312..9605)
                     /gene="yajC"
                     /locus_tag="PXD81_000578"
     CDS             complement(9312..9605)
                     /gene="yajC"
                     /locus_tag="PXD81_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005713354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HDL5536638.1"
                     /translation="MQQGGGMEMIFILIIFGAIFYFMIYRPQAKRQKQQRDLLASLTK
                     GDEVLTSGGLIGKITKVTADNDNIVIALNDTTEVTIKRDFVVAVLPKGSLKSL"
     gene            9806..10363
                     /locus_tag="PXD81_000579"
     CDS             9806..10363
                     /locus_tag="PXD81_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005604815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HDL5536639.1"
                     /translation="MKKLFAQAVIFGAFFASSAFAHNVQLNQALPAVSVAKDGELAVA
                     GGKVSYKNWHSSSLAGKVRVIHHFAGRSSVKEKNEDLMTAIKNAGFDRSKYQTVTIVN
                     ADDAIVGTGVFVKNSVENGKLDNPHSQVVLDQQGSVKKAWGLKEKESFIAVLDKSGKV
                     QFTSEGKLSAKQIQEVIGLVKTLTQ"
     gene            complement(10404..11372)
                     /locus_tag="PXD81_000580"
     CDS             complement(10404..11372)
                     /locus_tag="PXD81_000580"
                     /EC_number="5.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HDL5536640.1"
                     /translation="MKLFTLENAFLRITLSDVGASWLSCIVKMPNEEREVLVTTSAKN
                     WQKQTAFFGATIGRYANRIANAEYRLNGKTYQLAKNNGEHNLHGGELGADKIVWQLES
                     QSEQAVKFSYFFADGEEGFGGEVKAFVEYRLTENELQISFDAVANQDTPLCLTNHAYF
                     NLQGSETILDHELIINADGYLPVDASGIPNAPLKKVESTSFDFRNAKKIGQDLLADDD
                     QKAVKGYDHSFHLAKNSENPTACLAVEDLKMELRTSMPALQLYTGNWLKGQPNLTKGE
                     YEDYAGVALEPEFFPNSLNQPELLQFGGITKAGEEYHHTISYRFII"
     gene            complement(11498..12661)
                     /gene="galK"
                     /locus_tag="PXD81_000581"
     CDS             complement(11498..12661)
                     /gene="galK"
                     /locus_tag="PXD81_000581"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HDL5536641.1"
                     /translation="MKPQEIALQQFEQHFGYKAERTVFAPGRVNIIGEHTDYNDGFVM
                     PCAINYGTAVSFAKRSDNKWRVFAIDLNQQDEFDLSQAIEPSEQKWANYVRGVVKFIQ
                     AKHPEFKQGADLAITSDVPMSSGLSSSAALEVSIGKTAQLLGDLPLSLPQIALIGQEA
                     ENKFVGCNCGNMDQLTSALGQQDHLVMIDCRSLEITPTPVPQGYSIAIINSNVKHDLV
                     TGEYNSRREQCEFAAHFFGKAKLRDVTAEQFIERASELSEENELAYKRAKHIISENQR
                     VLEAVEALKANDMVKLGELMAQSHDSMRDDFEITCPEVDCLAEIAQQAIGKNGGARMT
                     GGGFGGCVVCLVPNDKVEDLRQAVANNYEKQTGIKETFHVCTASDGVRECPAR"
     gene            complement(12671..13717)
                     /gene="galT"
                     /locus_tag="PXD81_000582"
     CDS             complement(12671..13717)
                     /gene="galT"
                     /locus_tag="PXD81_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HDL5536642.1"
                     /translation="MSEFVLNDHPHRRFNPLKNQWILVSPHRAKRPWQGQQEEIVQDE
                     KPSYDPSCYLCPGNKRITGEQNPNYTKPFVFKNDFSALLPDTPAPIPNDDPLFQISHT
                     QGESRVICFSPDHSKTLPQLSIEEITDVVTVWQEQANELKEHYQWVQIFENKGSMMGC
                     SNPHPHGQIWASNFLPNEIAIEDECQAKYFAQYGSPLLLDYAQRELDKQERIVAETED
                     WIAVVPYWAAWPFETLLLPKAKQFKSITDLNEAEKADLALALKKLTTRYDNLFNISFP
                     YSMGFHFAPFNGKENAHWQLHAHFYPPLLRSATVRKFMVGYEMMAESQRDLTPEQAAE
                     RLNAVSDTKHYKDK"
     gene            13930..14781
                     /locus_tag="PXD81_000583"
     CDS             13930..14781
                     /locus_tag="PXD81_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF817 domain-containing protein"
                     /protein_id="HDL5536643.1"
                     /translation="MERLNKFDAWLMNHKPNARLSGASRLLTEFWFFGLKEARACLFA
                     GLFFIAMFSVPKTGLWGLPRYDILLIFAIVVQIAMVKLGLETWDELKAISLFHLVGFV
                     LEVFKTSSNIQSWAYPDFAYTKLFNVPLFTGFMYAAVGSYIIQAWRLFDLKIKSHLPY
                     WLATLTALMIYLNFFTHHYIGDYRWYIAAFALGLYARTFVFFTPYDITRKMPLLLSFF
                     LIGFFIWLAENIGTFAGIWRYPNQFGAWSMVHMSKWSAWALLVMMTFTIIVHLKHIKQ
                     TVRVSSD"
     gene            complement(14867..15952)
                     /locus_tag="PXD81_000584"
     CDS             complement(14867..15952)
                     /locus_tag="PXD81_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDL5536644.1"
                     /translation="MEYRYYITTALVIAVLQLFLFNFAKTVSWLFNLSSKNRKTVGIL
                     TFLVANGLVILHLTRTFALFRIMAFILVLLLFAFFISFFTACIYKIFNGNTTVNRWLK
                     IAYPFAFFGLLGLGYYNAYTPTVVHYSVKLDKPLEKPLRIGMASDLHLGKLFGTKQLD
                     QLAEIFNQQKVDLILLPGDIMDDNTEVYVADKMQPHLAKLKAPLGVYATLGNHDFFGA
                     QETIAKEITDAGITVLWDQTAEINGKFTLIGRNDDLVKNRPSAEQLLQNVNTKLPVIL
                     LDHRPTEIEQHAKLPIDIQVSGHAHKGQVFPASIITKLIYRLHYGYEKIGFGHYFVTS
                     GYGFWGVPMRLGSQSEVMIIDVEGENP"
     gene            complement(15974..16954)
                     /locus_tag="PXD81_000585"
     CDS             complement(15974..16954)
                     /locus_tag="PXD81_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HDL5536645.1"
                     /translation="MLFDSIQFSIGVTLPTILLMLFGIFPRRRKFIDDTFCNMASKVV
                     FNFTLPPMIFLNMLKSPLDFSSQINLISTGVGGSILIYLWAEWWAARHIENRRYRCIF
                     TQGTFRTNAAILGIALILNAYGDKALAAASVYISCLVITFNVLAVITLTSSLSEKKVN
                     FGRLILSILRNPLILAVIAGLTFNYFELSHQIPTPLESAGKLMASLTLPLALICTGAS
                     INFKQLKQFNQGVESTINKIVLFSASIRLIFAPIFLLLLGKFVFQLPPMELGIVFVAA
                     SAPVASATYAMTRNYGGDGEAAANLIGITTLGSMFSASIGLFLLRQIGWV"
     gene            complement(17057..18155)
                     /gene="prfB"
                     /locus_tag="PXD81_000586"
     CDS             complement(join(17057..18079,18081..18155))
                     /gene="prfB"
                     /locus_tag="PXD81_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014550889.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HDL5536646.1"
                     /translation="MFELNPIKNQLTDLSERTAVIRGYLDFEHKVERLEEVNAELEQP
                     DVWNKPEKAQALGKERAMLEQIVNTIKALEQGIEDVEGLIELAVEAEDQDTFNEAQVE
                     ADELEKKLADLEFKRMFSGPHDSADCYLDLQAGSGGTEAQDWTEMLLRMYLRWAESKG
                     FKAELMEASDGDVAGIKSATVKISGDYAYGWFRTETGIHRLVRKSPFDANNGRHTSFS
                     AAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNKTESAVRITHIPSGIVVQCQN
                     GRSQHQNKDQAMKQLKAKLYELEMSKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DHRTGVEHRTTQQVLDGDLDKFVEASLKAGL"
     gene            18411..19319
                     /locus_tag="PXD81_000587"
     CDS             18411..19319
                     /locus_tag="PXD81_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HDL5536647.1"
                     /translation="MAKRPSDIDATLLQLEILRRIPKLPAKIDAKSLHQQLLDAGFER
                     DIRTVQRSLKMLCEHFDIECDERSKPFGYSWKIRSEGLALPILNEQQSLLLKLAEQQL
                     KYLLPANLMASMKPFFEQADRMVSGAKDKPEYQWLNKVCSTPTSLPLIPAKIKEEIFN
                     SVSQALFQNKLLKIEYQNQHGKKHQATIMPLAISQQGASIYLVCRYEGFDDNRLLALH
                     RIRKAEISTFSFERPKDFNLQAYQDAGHLGFGNGEKASLTFSISRDAGFHLTETPLAK
                     DQKILEESETHYRIQAIVADNDMLTW"
     gene            19620..20261
                     /locus_tag="PXD81_000588"
     CDS             19620..20261
                     /locus_tag="PXD81_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HDL5536648.1"
                     /translation="MPFQLTFCQQAGNQHQKNQDALFNGKAVYQWQLKNAESYLLDND
                     KVIFGVADGVSSHPRNHLASHYLMALLSQCQSLDSQWLRQAHGDFCEKLAKDYFGASC
                     TFVACELHHNGRGRMVNVGDSRAYKITANGEWQQLSFDHTILAEMKQKGLVKEGSNYA
                     SMYNGLTDCIVADFDADDFRIHIAEFQLEKGESILLCSDGLHEQLAPEAIQAI"
     gene            complement(20245..20409)
                     /locus_tag="PXD81_000589"
     CDS             complement(20245..20409)
                     /locus_tag="PXD81_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5536649.1"
                     /translation="MRILSTNATNNVVLITQFHKSPQKNRHTSGAFLPFFCKFADDET
                     GFRIRPKSPE"
     gene            20428..>20633
                     /locus_tag="PXD81_000590"
     CDS             20428..>20633
                     /locus_tag="PXD81_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005637830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacetylase"
                     /protein_id="HDL5536650.1"
                     /translation="MKNDFHYAAELIRNADGILITAGAGMSVDSGLPDFRSVGGLWNS
                     YPPLKKFNLQFMQIATPLAYQERP"
BASE COUNT         5914 a         4260 c         4424 g         6035 t
ORIGIN      
        1 tctcaaattt ttagcttttt tgataaaaaa gagccttcaa aagcacaaga aaaatcgacc
       61 cagagtagtc agtctcatca agtttcaact tcatcactag ggaactatga tgtgcagatg
      121 aaaaacgacc gtttgaaaca gcatattgta gataccgatt attacacctt agcgttgtcg
      181 tggtcgccgg cgttttgcca aaaacaaaag cgaaataatc acggtaaaat tccgtctcat
      241 ttggaatatc aatgtggtgg ctcagcggat tttggttggg ttattcacgg cttatggccg
      301 caaagtggca aggcaagaag tgttgatgaa caccctcgtt attgccaagg cgatttgccg
      361 atggtggcag agcagacgat taagcaatat ttacctgaat cgccgggggc aactttgttg
      421 caaggcgaat gggaaaaaca cggtgcttgt gcctttaacc aagcggatga ttacttcgcc
      481 aaacaaaaag agctatttaa tcagctcaaa ttaccggata ccgaaatgtc taaattagca
      541 ctgtttaact ggatgcgagc caataaccct catttgcaaa atgtctattt agggggtagc
      601 aaagatgagc tgtatatttg ctatgataaa gcatttcaac caatggattg cccacgctaa
      661 tggcagatct tcgccaactg aaaatgcagg ttcagcgcca attaaagcgt gatcttgaac
      721 gtgcaaatca ctattttaat accactttta cgccaccaag tattagctat gcggtgcgtg
      781 gcgtaaaagc cggtgtggct tatcttgaac gcaacgaagt acggtttaat cctgtgttat
      841 tgtaagaaaa tgaacaggca tttatccgcc aagttgtgcc gcacgaactg gctcatatct
      901 tggtttatca acattttggg cgggtacaat ctcatggtaa agagtggaaa atgatgatgg
      961 aaaccgtact gggtgtgccg gcggaaattt accattgttt tgatacccaa agtgtgcaac
     1021 agcaatttag ttaccaatgt gcctgccaga ctcaccagct ttcgattcgc cgacacaatg
     1081 ccgtgatgcg agataaaaaa agctatattt gccgtttatg taaaactcaa ctccattttg
     1141 tagaaaaaag ttaatgtgga gaaaagttaa gagaatgtta cgatttagct agacattcct
     1201 gtttaaacgc ctacaatttg ccgtagtttt tatttttaat tttatttccg gagtatttat
     1261 ggaaacaatt gtaattgtag gtggcggtgc aggcggtttg gagcttgcaa cctatctagg
     1321 caacaaatta gggcgtaaac aaaaggcaaa agtggtgctg attgaccgca atgcaacgca
     1381 tttatggaaa ccactgttgc acgaggtggc aaccggtact attgatgagg gaactgatgc
     1441 gttaagctat cgggctcacg ctaaaaatca tagttttgaa tttcagcaag gtacgctggt
     1501 ggcggtagat agagagaaga aaacggtttc actcgccccg atttataatg atcaaaacca
     1561 acttctcgtt aaagaacgcc atatcagtta tgacaaatta gtgatcgcga tcggcagtaa
     1621 atccaatgat tttggcacca aaggggttgc agaaaattgc attttcttag atggctcaga
     1681 gcaagcaaaa gatttccaaa aacgaatgat ggagctgttt ttacgttttt cacatagtga
     1741 aaataaagat gtaaaaattg cgattgtggg ggggggagca acaggtattg agctttcagc
     1801 ggagctttac cacgtggtga aaaatttaaa ttcttacggc tttggtaaat taaaccgtgc
     1861 cagcttgaaa gtcactttaa ttgaggcagg ccctcgtttg attccggcat taccggagaa
     1921 agtgtcggtt tctgctttcc acgaattgaa aaaagcgggt gtttcggtgc gtttaggtac
     1981 gatgattacc gaagctcgtc cagatgggct aatgacccgt ttaggtgaga aaattgaggc
     2041 ggatattatg gtttgggcgg ctggcgtgaa agcccctgaa attacccgtg aatttggttt
     2101 tgaaactaac cgcttgaacc aaattgagat taaagacacg ctacaaacca ccgttgatga
     2161 gtcagtgttt gtcattggcg attgtgcatc attaattcaa gatggcaagc caattccacc
     2221 cagagcacaa gcagcccacc aaatggcaac ccgatgtggt aaaaatattg tggcattgat
     2281 agaaggaaaa gagatgaagc cgtttcgatt taacgataaa ggctcactgc tttccttctc
     2341 aaaattcggc acggtgggta atttaatggg gaatttagtg agcggtgata tgtttattga
     2401 aggtaaaatt gcccgcttgg cttacctctc gctttatcga atgcaccaag tggcattaca
     2461 cggctgtgtc aaaaccggct tgattgtgtt ggtagggcaa attaaccgtt tcttaagacc
     2521 aacaatgaaa ctacattaaa caaaaatccg catcaaatga tgcggatttt ttatggcaaa
     2581 ataacaagcg gttattttct tgcctatttt tgcaaatact cacgcctagt ttgctaattt
     2641 aatttccgcc aaccatttat caattaagcg tttgccctct tcgctggaac ctgctttttg
     2701 gtaatcaatt tccacaaagt ctaacgcttc ggctttggtt gattgtggag caacttcggc
     2761 gttgatgttt gttggggttt ggtaaacacc gtgttttttc caaggaattt cctgtgcttc
     2821 ttttgaaagc acccaatcca taaagagttt ggcgttatct aaattacggg catttttgat
     2881 aatgctgaca ccgcctaacg catagcccac tttaccttcc ggaataatgg cttcaatagg
     2941 cgcacctttt tctttttcag tggcataact atgtacaaag cctaccgtaa ccgcactttc
     3001 accacgagca agattgctgg tgactagggt gcttttcaca tattgcgaaa cgttattgtt
     3061 gagttttttc agataatcaa aggttttctc ttcgccccat aaggttgaaa gagtggtcat
     3121 aaacgtataa gtcgtgccgg agctacgagg atcaggcagt tgtacttcac cttttaagcg
     3181 aggatctgat aaatcttccc attttttcgg tgcctcaatg cctagtttgg cgagtttttc
     3241 ggtattcacg cctaacccta atacaagcat ataaacgata gaggtaaatt taccttgttc
     3301 actttcaact aaggatttaa attgtggcag aatttcagct tgttttggtg aacggtaggc
     3361 ttccagtaag cctaattcgc ctgcttgaaa gtgaggttca atcgtgccgc cataccaaat
     3421 atctgcctga gggttcgatg cttccgcttt tacttttccg aaaattgtac cggttccgcc
     3481 gtgaatgaat ttagtttcga cattatattt gtgtgaaaat tgcttggtca tttcttcaca
     3541 aacattattt tgtaccgagc aataaacagt taaacgacct tctgcttgtg ctgcttgagc
     3601 tgataagaaa atgccagagg cgattaagat cgattttaat gagattaatt tgagtacgtt
     3661 catcactttc ctgaaaacat aaaataaaag gtagtgaagt ctagcatttc aaagcgctgg
     3721 ttgaaaatag ttaattgcaa tgaagtaggg caattgtgcc tattacttta atatttacca
     3781 actatttgca agttttagca aaaaataacc gcttgtagta gctcttgcta ttttgtcaaa
     3841 attcagaata ataaatgcct ttagtctaat gttaaatttt agttatggaa aatagttgga
     3901 aatatgcttt taaattaatc acgccgattt ttatgggcta ttttgcggca ggggttgctt
     3961 acggaatgct tgccaccaat gccggaatgc ctgcgtggtt tacgattgta atgagtttta
     4021 ccgtggtatc ggggacggca caatatgcag cgattccatt ttttgtctcc ggtgttgggg
     4081 cggtgtcggt ctttctcagt acgcttttaa tgagtctacg ttttagtttt tacaccttaa
     4141 atatgatgga gcataagccg atatccaaag cgaaaagtct ggtcgctatt gcaggtttaa
     4201 ctgatgaagg ctttgcggtg ttatcttcat taccgaaaga gcaaaagcaa gcattattct
     4261 ttaaaatcgc tgtgctatgt gtggcttatt ggacattttc cacgattgtc ggtgtgttgt
     4321 taggcgattc agtggcgaac tatattccgc atttggattt tgctctgcct tgtttgcaat
     4381 tttagcctat gagcaataca aaaaccaaaa acaatggaaa ccgattgtga ttgcgttaat
     4441 cggattttta cttgctcgtc agattaccga aaccagcgtg ttattggtgg caattttagt
     4501 agcgattgtc attgtggctt tattacctca atcttcttca atcaaactag agaaaggagt
     4561 agaaaatgag cacaaatgat actttgataa tgtttgcagc attagtgctc ggctcgcaaa
     4621 tttgtcgttt tttgccggaa tttttaccga aaaaggtgtt atcttcgccc attttgcaaa
     4681 aattaaacaa aatgttaccg cttgtgatta tgcttttatt gcttttaacg agcctaaact
     4741 ttcctaaaaa cggggaaggc tttaacttaa tattggcaca aattctggct ttaataggcg
     4801 tattaggtag ctatgtttgg cttaaaaata cctttttaag cgtagcgttg gggattttcg
     4861 gtgtgaatgt atttttatcc ctgttaggat aaataccaaa ggtcatttta taggagcgaa
     4921 acaccttcgc tcaatcatta acagatgtgg ggcgaaaaat ttttcgcccc ttatttatca
     4981 caagcggtct ttttctttca attttttgca aattaaatcg ctaaatcaaa cgcatagaag
     5041 gcaactaaaa taatcgcaat aattactgta cctaggttta atttattggc ttcgcctgaa
     5101 attaagcgac caatcacaag ggtggcgaaa cctagcataa tgcctgtcac aatgttggcg
     5161 gttaatacga tgaacaccgc acagactaaa cctgacattg cacctacaaa atcgctgaaa
     5221 tctaatttag agacattact tagcattaat aagcccacat acattaatgc cggtgcggtg
     5281 gcatagcttg gtactaagaa tgcaagaggt tggaagaaca gcataaataa gaaaccgata
     5341 ccaaccacta cagcggttaa acctgtttta ccgccaacgg ctgtacctgc tgccgattca
     5401 atatacactg ccgctggtgc tgtaccaaat aagcccgata aaattgatcc gaatgagtca
     5461 gaagttaggg ctttatcgcc attgataatt tgtccgtctt tatctaataa accggcttgc
     5521 cctgcaaccg cacggatagt acctgttgca tcaaaaatag cggtcattac taacgcaaac
     5581 actaccggta aaatggcggt atttaatgct cccataatat caagatctag gaattgcgaa
     5641 ttttcaccaa aactcggcat tttgaaaaag cctgtgtatt ttacgttagg gtcaaaaatt
     5701 aaacctacga tggtaatagc aataatgacc cataaaatac tgccttttac tttcagcttt
     5761 tctaacccga taatggcggc tagaccgatt aatgacacta aaaccggaaa tgaagtgaag
     5821 tcccctaatt taaccggaat atccataccg ctattgacca ctaatttaac gttagtggcg
     5881 gcaataatca gtaagaataa accaatacca attccagcac cgtgggcaat gctgcttggc
     5941 aaattacgta aaatccaagt acgaataccg gtagcagaaa tcaaggtaaa tactacgccc
     6001 attaagaaaa tcgccccgag tgcaacagga atagaaactt gttgccctaa tactaagcta
     6061 aaggcagtaa aagcagtaag tgaaatcgca caaccgattg ccattggggc atttgcccat
     6121 aaaccgatta agatagagcc gagacctgaa actaaacagg tggcaataaa taccgattcg
     6181 gcaggaaagc ctgcttggct tagcattccc ggcacaacga tgacggaata aaccatggct
     6241 aaaaaggtag ttaaaccggc gatgatttct tgacgagcgt ttgaaccacg ctcctcaaat
     6301 ttaaataacg acataagaac ctcaaaagaa gaaaatgaaa ggggaaattt taggatgcgg
     6361 aattttacag aaatttaaaa taattagcaa acgtttgcgt aaaaagtgaa agaaaagcag
     6421 gaataaatcc tgcttttgtt tgatggttag taatcgatga aagcgtcttg atctgcccct
     6481 tccttttcaa ctttaggctg aaccatatgt tcacgtttta gcccaagttg tagtgccaca
     6541 ccaattgcaa tgtagatgga tgaataagta ccgaaaccga taccgatcaa taatgcaagt
     6601 gagaaactat gaagcgttgg accacctaga tagaataagg ctagtaccac aaagagggtg
     6661 gttactgatg tcattaaagt acgcgaaagt gtttggctca aggaaatatc gataatttca
     6721 attgagctgg agcgacgaac tttagaaaag ttttcacgta cacggtcaaa caccacaatt
     6781 gagtcattta acgagtaacc gacaacagat aaaatcgctg ctacaaaggt caagtcaatt
     6841 tcaatctgta agaaagagaa gattccgatc gtcactaata catcgtgaaa taaagccaca
     6901 ataccgccta ccgctaaacg ccactcaaaa cgtgttccca cgtaaataag tagcatagca
     6961 agtgttgcca aagtggcata aatagcccct tgggttagct cttcccccac gtttgggcca
     7021 acgaattcaa tgctcttgat agtcgcattc ggatctaatt tttgatggat catctccatc
     7081 actttattcc caacagacat atcaccggca ctggcggcaa gtcggatcat taaatctttt
     7141 tgactgcctg tggtttgcac taaggcattg ccgtagccgc tattttcaag ctccgtacgc
     7201 agttttgcta aatcagcagg ttgtgaaaat tgagtttcga ttactgtccc gccggtaaaa
     7261 tctaaccccc agttaaagcc ttttgtcgca atagtaaaaa tgcataaagc agttacaatc
     7321 agcgaaaaaa tgaatccgac atagcggtat ttcataaagg ggattaattt ataaggcagt
     7381 ttaatttctg ctacatcttg ttgtacattt tcaatagcca tttttccacc ttaaatccaa
     7441 agtttcttca cgcgtttgcc accgtaaacc cagtttacaa gcattcgagt acctgtaatt
     7501 gcggtaaaca tagaaataat tacccctaat gcaagcgtta ccgcaaagcc ttttaccgga
     7561 cctgtaccta caccataaag cacaagagaa gtcaaaatag tggtgaggtt ggagtcaaaa
     7621 atacttgaga atgcgccatt ataaccttcg tggattgctt gttgaattgg gcgaccgtta
     7681 cgaatctctt ctttgatccg ctcgtagatt agtacgttgg catcaactga catacccaca
     7741 gcaagtacga taccggcaat tcccggcata gtaagggttg caccgattaa tgacattaaa
     7801 ccaatcgtta agatcatatt cacaatcaag gcaagagagg caaatatacc aaatacttta
     7861 taaaaggcta gacagaacag gatcgttaag cctaagccta aaatactagc ttccatacct
     7921 tgtttaacat tatctgcccc aagagaagca ccgatagtac gctcttccac aatcacgatt
     7981 ggagcaatca acgcacctga acgtaataat accgcaaggt tttgagcttc cgctggtgaa
     8041 ttaatccctg taatctggaa ttgattacct aaacgggttt gaatggttgc aacgttaatt
     8101 acctcttggt gtttttctaa aatgacttta ccgttagcat ctttacgacc tgaatcttta
     8161 aattcggtat aaagtgttgc catcggtttt tggattgcca ttttagtaat ttcacccatc
     8221 atctcgccgc cttcggtatc gagtcggata ctcacttgcg gcaagccacg ctcatcagtg
     8281 cctgaggttg catcaataat atgttcaccc cctaacgaag gtttacggcg taatacaact
     8341 ggctcgccat cacgggtgtt ttgcagtcct gaatcagccg gcacaatgcc tttcgccgca
     8401 gaggccacat tggcttcggt attgactaac cggaactcaa gggttgcagt tgcccctaaa
     8461 agctctttgg cacgagcggt atcttgcaca cccggtaatt caactacgat acgatctgca
     8521 ccttgacgct gaatggtcgg ctcagaaacg cccaattcct caacacgttt acgcaaaata
     8581 gaaaggtttt gttcgattgc catcgtgcga gattcagaaa gagcttgtgc tgatggcgtt
     8641 aaggtgactt tattatcatt taccaaaata tcaagcgttg ggtgagagag acgcactaaa
     8701 cgcactgctt tttcggcggt atcgttatcg gcaaattcaa cctcagttgc aaaattttcg
     8761 ccttttttga ccgcttgata ttgcaatttt tctttacgga aatcactacg caggctgtct
     8821 tgcaaagtgt cttgttgttt tgcaagcgcc gtgttcatat ccacttccat taagaaacga
     8881 acaccgccaa gcaagtccaa gcctcgcttc atcgggctac cgccaatgtc agacaaccac
     8941 tgtggcgttg ctggtgcaag gtttaaggca acagagtagt tattccccaa tgcttctgag
     9001 attttttctt tggcaggcaa ttgctgttcg ctgctttcga gacgaaccaa aatcgagcca
     9061 ttttctaaca cggcagattt cggctgaata ttcattgctg aaagtgtgga ttgcacttga
     9121 gacagtgtct caggtgtggc ttgttgccca cgagtaccgg aaatttgtac cgaaggatca
     9181 tcgccatata aatttggaag ggcatataaa gcaccaatgg cgatcacgaa gatcaccatt
     9241 aaattcttcc atagagggaa acggtttaac acaataactc cccgaatgtt aaagttggat
     9301 aagagggttg attaaagtga ttttaatgaa ccttttggta acaccgcaac aacgaaatca
     9361 cgtttgatgg tcacttcagt tgtgtcattt aatgcaatca cgatgttatc gttatcagcg
     9421 gtcactttgg taattttacc gattaaacca ccgctggtta atacttcatc gccttttgtc
     9481 aagcttgcca gtaaatcacg ttgttgtttt tggcgttttg cctgaggacg ataaatcata
     9541 aaatagaaaa ttgcaccgaa aataatcagg ataaagatca tttccattcc gccaccttgt
     9601 tgcataaaaa ctccttaaat gtttgagtgg tataaaaata agtgttataa aaaacgcctg
     9661 ttaaaatacc ataaattcaa tgaattaacg agtagaaaaa ttcatcgaat gcttttcttt
     9721 taaacaaaaa tttgagtaga atcagcactt ttagtttagg attaggttct tggcgtgttc
     9781 ttgcgctgac acaataggag aaaaaatgaa aaaattattc gctcaagcgg ttatttttgg
     9841 ggcttttttt gcaagttccg cttttgctca caatgtgcaa ttaaatcaag cattacctgc
     9901 tgtttcggtt gcaaaagacg gggaattggc agtggcaggt ggaaaggtta gctataaaaa
     9961 ttggcattca tcatctctgg caggtaaagt ccgtgttatt catcattttg ccggtagaag
    10021 ttcggtgaaa gaaaaaaatg aagatttgat gacagcaatt aaaaatgccg gttttgatcg
    10081 ctcgaaatac caaacggtga cgattgtaaa tgctgatgat gcgattgtcg gcacaggggt
    10141 atttgtgaaa aatagtgtgg aaaacggcaa acttgacaat cctcacagcc aagtagtgtt
    10201 agaccagcaa ggcagtgtga aaaaagcgtg gggcttaaaa gagaaagaga gctttattgc
    10261 agtgctggat aaatcgggta aggtacaatt tacctctgag ggcaaactct ctgcaaaaca
    10321 gattcaagaa gtgattgggt tggtgaaaac attaacgcaa taaccagtgt ttttatgggc
    10381 ggattttgta tccgcccatc attctagata ataaatcgat aacttatcgt gtgatgatac
    10441 tcttctcctg cttttgttat cccgccaaat tggagtaatt caggctggtt tagtgaattt
    10501 gggaaaaact caggctctaa tgctacacca gcgtaatctt catattcgcc tttagtcaaa
    10561 ttaggttgtc cttttagcca gttgccggtg taaagttgta atgctggcat ggaggtgcgg
    10621 agttccattt ttaagtcttc cacggctaaa caagcggtcg gattttcact attttttgca
    10681 agatgaaagg agtgatcgta accttttacc gctttttggt cgtcatcagc caataaatct
    10741 tgcccaattt ttttagcatt gcggaagtca aaactggtac tctccacttt tttcagtgga
    10801 gcattaggaa taccgctggc atcaaccggt aagtatccgt ctgcattaat tatcagctcg
    10861 tgatctaaaa tcgtctctga accttgcaaa ttaaaatagg cgtgattagt taagcaaagc
    10921 ggtgtatctt ggttagcaac ggcatcaaag ctgatttgaa gctcattctc tgttaagcga
    10981 tactccacaa aagctttcac ttcgccgcca aatccttcct cgccatctgc aaaaaaataa
    11041 ctaaatttga ccgcttgttc gctttggctt tcaagttgcc acactatttt atctgcccca
    11101 agctcgccac cgtggagatt atgttcaccg ttatttttag cgagctggta ggttttgccg
    11161 tttaagcgat attcggcatt ggcaatgcgg ttagcgtaac gcccaatggt cgctccaaag
    11221 aaagcggtct gtttttgcca attttttgca gacgtggtga ctaatacttc ccgttcctcg
    11281 ttcggcattt tgacaatgca cgaaagccaa gaagccccaa catcagaaag ggtaatgcgt
    11341 aaaaacgcat tttcaagggt gaataatttc atcggttgac cttaattacc tagcacggca
    11401 tagccgtact aggttaagca taattcagcc ctgctagggc tgaggttatt tattttagag
    11461 tagcggagct acttccttat gcttaacccg gtacgcttta gcgtgccggg cattctcgca
    11521 ccccatcgga ggcagtacaa acgtggaagg tttctttaat gccggtttgc ttttcgtaat
    11581 tgttagcaac ggcttggcgg agatcttcca ctttgtcatt tggcactagg cacacgacac
    11641 aaccgccaaa gccgccgcca gtcattctgg ctccgccgtt tttaccgata gcttgttggg
    11701 cgatttcggc taagcaatcc acttcagggc aggtgatttc aaaatcatca cgcattgagt
    11761 cgtgagattg agccatcaac tccccgagtt tgaccatatc attcgctttt aaggcctcta
    11821 ctgcttccag aactcgttga ttttcgctga tgatatgttt tgctcgttta tacgccagct
    11881 cattctcttc actaagttct gaggctcgtt caataaattg ttccgctgtg acatcacgca
    11941 attttgcttt gccgaagaag tgggcggcaa attcgcactg ttcacggcga ctgttgtatt
    12001 cgccggtaac caagtcgtgt tttacgttgg agttaataat cgcaatcgaa tagccttgtg
    12061 gcacaggggt tggggtgatt tctaaactac ggcagtcgat catcactaaa tgatcttgtt
    12121 gcccgagagc ggaggtgagc tgatccatat tgccgcagtt gcagccgaca aatttattct
    12181 cagcctcttg cccgattaat gcgatttgcg gtaaagaaag cggtaaatcg ccgagtaatt
    12241 gagcggtttt accaatcgac acttccaatg cggcggaaga acttaaacct gatgacatcg
    12301 gcacatcgct ggtaatggct aaatctgctc cttgtttgaa ttcggggtgt ttggcttgaa
    12361 taaatttcac cacgccacgc acataattcg cccatttttg ctcgcttggc tcaatcgcct
    12421 ggcttaaatc aaattcatct tgttggttga gatcaatggc gaacacccgc catttgttgt
    12481 cactacgttt ggcaaagcta accgctgtgc cgtaattgat ggcacaaggc attacaaacc
    12541 catcgttgta atcagtatgt tcgccgataa tattcactct gcccggggca aataccgtgc
    12601 gttctgcttt ataaccaaaa tgttgttcaa attgttgcaa agcgatttct tgtggtttca
    12661 tttcaattcc ttatttatct ttatagtgtt tggtatcact tactgcattt aaacgttcgg
    12721 cagcttgttc cggggttaaa tcacgttggc tttctgccat catttcataa ccgaccataa
    12781 atttacgcac tgttgccgaa cgcaagagcg gcggatagaa gtgagcgtgt aattgccagt
    12841 gagcattttc cttgccgtta aacggagcga agtgaaagcc cattgaataa ggaaagctga
    12901 tgttaaacag attgtcgtaa cgggtggtga gctttttcaa tgcaagggct aaatccgctt
    12961 tttccgcttc gtttaaatcg gtaatggatt tgaactgctt ggctttcggc aacagcaagg
    13021 tttcaaacgg ccaagccgcc cagtaaggca caaccgcaat ccaatcttcg gtttctgcca
    13081 caattcgctc ttgtttgtct aattcacgtt gtgcgtagtc aagcaacagt ggcgagccat
    13141 attgagcaaa atatttcgct tggcattcgt cttcaatcgc aatttcatta ggtagaaaat
    13201 tacttgccca aatttgcccg tgtgggtgag ggttagaaca ccccatcatt gagcctttgt
    13261 tctcaaaaat ttgcacccac tgataatgct ctttcagctc attcgcttgc tcttgccaaa
    13321 ctgttaccac atcagtaatt tcctcgatgg aaagttgcgg taaggtttta ctatgatcag
    13381 gcgaaaaaca gatcacacgg ctttcgcctt gtgtatgaga aatctgaaac agcggatcat
    13441 cattcggtat tggtgccggt gtgtctggta gcagggcaga gaagtcgttt ttaaacacaa
    13501 acggcttggt gtaattcggg ttttgttcgc cggtaatccg cttattgccg gggcagaggt
    13561 aacaacttgg gtcgtagctc ggtttttcat cttgcacgat ttcttcttgt tgcccttgcc
    13621 acgggcgttt tgcccgatgc ggagagacta aaatccattg attttttaat ggattaaaac
    13681 gacgatgtgg gtgatcgttg aggacaaatt cgctcatata aaactcctcg aaattagtta
    13741 tgtaaagagt atagcgaaag tcagacaaaa tgactgtgac atagctcaca aaataatgat
    13801 gtcaagcggt caaatttcag aaaaattttg caagttttcc tccattttgc gatttaaggt
    13861 agcttacgtg gaattgaatg tggcttttgg tagaatagat gaatatgcaa gatattaaga
    13921 caaaaataga tggaaagact gaataaattt gatgcttggt taatgaatca taaacctaat
    13981 gctaggttaa gcggagcgag tcgtttgttg acagagtttt ggttctttgg gttaaaagaa
    14041 gctagagcct gtttatttgc gggattattt tttattgcga tgttttcggt accaaaaacg
    14101 ggattatggg gcttacctcg ttatgatatt ttgctgattt ttgccatagt ggtgcaaatt
    14161 gcaatggtga aactggggct ggaaacgtgg gacgagctaa aagcaataag cctatttcat
    14221 ttggttggtt ttgtgttaga agtctttaaa acatcgagta atattcaatc ttgggcatat
    14281 ccggattttg cctatactaa attgttcaat gtgccattat ttactggttt tatgtatgca
    14341 gcagttggtt cttacattat ccaagcgtgg cggttgtttg atttgaaaat taaaagccac
    14401 ctgccctatt ggttagccac tttaacggca ctgatgattt acctgaattt ctttacgcat
    14461 cattacattg gggattaccg ttggtatatt gccgcttttg cattagggct gtatgcacgt
    14521 acttttgtat ttttcacccc ttatgacatt acacgaaaaa tgccactgct gctttctttc
    14581 ttcctgatag gttttttcat ttggttggcg gaaaatatcg gtacgtttgc cggaatttgg
    14641 cgctacccga atcagtttgg ggcttggtct atggtgcata tgagcaagtg gagtgcttgg
    14701 gcgttattgg tgatgatgac ttttaccatc attgtgcatt taaagcatat caagcaaaca
    14761 gtcagagtct cgagcgatta acaagctgtg ggttttggcg aaaattttac tcatcagtta
    14821 ttttcagtgg aattaggggc aaataataat ttgcccctat atcaaattat ggattttccc
    14881 cttccacatc aataatcatc acttccgact gtgagcctaa ccgcatcggc acgccccaga
    14941 atccgtagcc ggaggtgaca aaataatgcc cgaagccgat tttttcatag ccgtaatgta
    15001 atcgatagat cagtttggta ataatacttg cagggaacac ttgccctttg tgagcgtgtc
    15061 ctgaaacttg aatatcaatc ggcaatttag catgttgctc aatttcagtc gggcggtggt
    15121 caagcaaaat taccggtagt ttagtgttca cgttttgtaa aagctgttcg gcagaaggtc
    15181 gatttttgac taaatcatca ttacgcccaa taagggtaaa ttttccgtta atttcggccg
    15241 tttgatccca taaaacagta ataccggcgt cggtaatttc ttttgcaatc gtttcttgag
    15301 ccccaaaaaa gtcgtgattg ccaagggtag catacacgcc aagaggagct tttaatttgg
    15361 ctaaatgagg ctgcatttta tcggcaacat aaacttcggt attgtcgtcc ataatatcgc
    15421 ccggcaataa aatcagatcg actttttgct ggttgaaaat ctctgccagt tggtcaagtt
    15481 gcttggttcc gaacagtttt cctaaatgca gatcgcttgc cataccaatg cgaagcggtt
    15541 tctctaatgg tttatccaat ttcaccgaat aatgtactac tgttggggta taagcgttgt
    15601 aatagccaag cccaagtaag ccaaagaatg caaatgggta ggcgattttc agccagcgat
    15661 tgacggtagt attgccgttg aatattttgt aaatacaagc ggtgaaaaaa ctgataaaaa
    15721 atgcaaaaag taacaatacc aatatgaatg ccattattct aaatagcgca aaagtacgag
    15781 taagatgcaa aatcactaat ccgtttgcga ctaagaaagt gagaatacca actgttttac
    15841 ggtttttact tgataaatta aataaccagc tgacagtctt tgcaaagttg aataaaaaga
    15901 gttgtaacac cgcaatcaca agggcggtgg taatgtagta acgatattcc atatttttcc
    15961 ttcaataaat tggctaaacc cagccgattt gtcttaataa aaataagccg atgctggcac
    16021 taaacattga gccgagagtc gtaatgccga tcagatttgc ggctgcctcg ccgtctccgc
    16081 catagttgcg tgtcattgcg taggtggctg aagcaacggg agcagaagca gccacaaaga
    16141 caatgcctag ttccatcggc ggaagttgaa acacaaattt cccgagtagt agcaagaaaa
    16201 ttggagcaaa aatcagccga atcgaggcag aaaacagcac aattttattg atggtgcttt
    16261 ccactccttg attaaattgt ttcaactgtt tgaaattaat gcttgctccg gtgcagataa
    16321 gtgctaatgg tagtgtcagg ctcgccatta atttaccagc agactcaagc ggggttggaa
    16381 tttggtggct taattcaaaa tagttaaaag ttaagcctgc aatgacggct agaatcagcg
    16441 gatttctcaa tatcgacaat attaatctgc caaaattcac ttttttctcg cttaacgatg
    16501 aagtgagggt aatcactgca agcacattaa aggtaatcac caagcacgaa atataaactg
    16561 aagcggcagc gagagcttta tcgccataag cattgagaat taaggcaatg cctaaaatcg
    16621 ccgcattggt acggaaagtg ccttgagtga aaatacaacg ataacggcgg ttttcaatat
    16681 gtcgagccgc ccaccattcc gcccataaat agatcaaaat cgaaccgccg actccggtcg
    16741 agatcaggtt gatttggctg gagaaatcta acgggctttt tagcatattc aagaaaatca
    16801 tcggtggcag ggtaaaatta aataccacct ttgatgccat attgcaaaag gtatcgtcaa
    16861 taaattttcg tctgcgtgga aaaataccga acagcattaa caaaatggtt ggtaaggtaa
    16921 cgccaatgct aaattggata gagtcgaaaa gcataatgtt tgccttaaat gatggcacga
    16981 gtgtcttttg cttgtaccgt agattatcaa taaagtacca gcgtggacgc tggtactatc
    17041 atcaatggaa atgaaattac aatcctgcct ttaagcttgc ttcgacaaat ttatccaaat
    17101 caccgtccag cacttgttgg gtggtgcgat gttccacacc ggtacggtgg tctttaatcc
    17161 gtgaatcgtc tagcacataa gagcggatct ggctgcccca gccgatgtcg gatttgttgt
    17221 cttccatcgc ctgtttttcc gcatttttct tgctcatttc cagctcatat agcttcgctt
    17281 taagctgttt catcgcctga tctttatttt gatgttgtga acgaccgttc tggcactgca
    17341 ccacaatgcc gctcggtata tgggtaattc gcaccgccga ttcggtttta ttgacgtgct
    17401 gaccacccgc ccctgaggca cgatacacat caatccgcaa atccgcaggg ttgatttcaa
    17461 tatcaatatc gtcatcgact tccggataaa cgaaagccgc cgagaacgag gtatgacgac
    17521 cgttattggc atcaaacggg cttttacgca ccaagcggtg aatacccgtt tcggttctga
    17581 accagccgta agcatagtcg ccggaaattt tcacggtggc ggatttaatc cctgccacat
    17641 caccgtctga ggcttccatt agttcggctt taaacccttt gctctctgcc caacgcaaat
    17701 acatacgcag taacatttct gtccagtcct gagcttccgt tccacccgaa ccggcttgca
    17761 aatccaaata gcaatcggca ctgtcgtgtg ggccgctgaa catccgctta aattctaaat
    17821 cggcaagttt tttctctaat tcgtctgctt ccacttgggc ttcattgaag gtatcctgat
    17881 cttctgcttc caccgccagc tcaatcaacc cttcaacatc ttcaatccct tgctctaagg
    17941 ctttaatcgt attgacgatc tgctccaaca ttgcccgctc tttaccaagc gcttgtgctt
    18001 tttccggctt gttccataca tcgggttgtt ccagctcggc attgacctct tctaagcgtt
    18061 cgactttatg ctcaaagtca aagatacccc ctgatcaccg cggtgcgctc gcttaaatca
    18121 gttagttggt ttttaattgg gtttaattca aacattgtcg ttcctgtttt tgcttatttt
    18181 aaaaagatga attatagctg aagttaagca agcggtcgaa ttttgcaaga aatggagcaa
    18241 tgatcgtttc tagtaatgag atttggttca taaaagttgc aactgtttct gctatttggc
    18301 tttgatagct aaaaatcttt aaaaaaacaa gcggtcgttt taccccattt tttgcaaaac
    18361 gaccgtagct tcttgctata atcgctgaaa aaatgctgca aggagatttt atggcaaagc
    18421 gcccaagtga tattgatgca accctattgc aactggaaat cctacgccgt atccccaaat
    18481 tacccgctaa aattgatgct aaaagtttgc accaacagct attagatgcc ggctttgaac
    18541 gggatattcg cactgttcag cgttcgctca aaatgttatg cgagcatttt gatattgaat
    18601 gcgatgagcg gagcaaaccc tttggttata gctggaaaat ccgctcagag gggctggcat
    18661 tgccgattct caacgaacaa caatcattac tgctaaaact tgccgagcaa cagctgaaat
    18721 atctgttacc cgcaaattta atggcatcaa tgaaaccctt ttttgagcag gcagacagaa
    18781 tggtttccgg ggcgaaagac aagcccgaat accaatggct aaataaagtt tgctccacgc
    18841 caaccagcct accgctgatc cctgcaaaaa ttaaagaaga gatttttaat tccgtaagcc
    18901 aagccttatt tcaaaataag ttgttaaaga tcgaatatca gaaccagcac ggcaaaaaac
    18961 accaagcgac aattatgccg ttggcgattt cacaacaagg tgcgagcatt tatttggtct
    19021 gccgttacga agggtttgac gacaaccgcc tgttggctct tcaccgcatt cgtaaagcgg
    19081 agatttctac ttttagtttt gaacgcccga aagattttaa tttgcaagcc taccaagatg
    19141 ccggacacct cggttttggc aatggcgaaa aagctagcct taccttttcg attagcagag
    19201 acgccggttt ccatttaacc gaaacaccgc ttgccaaaga tcaaaaaatt ctcgaggaga
    19261 gtgaaactca ctaccgtatt caagccatag tcgctgataa tgatatgcta acctggtagc
    19321 tacgcaaatt tggcgaagat atttgggata tcgagaaaga agcgctaaaa taagctaaac
    19381 aagcggtcgg ttttgtggga atttttgcta aatagtatcg accgctcctc cctaaacaga
    19441 cctttatcat aaatacaact ctttcaatca gatattctta ctgtctattg atgaacgatt
    19501 cgaatttttc tagtggttga aatttaaact aattttcttg ctttttggca tttttcagac
    19561 ttttaattct ctccattagc ctgttttttt acgctctttt tcataaacag gaataaccaa
    19621 tgccttttca acttactttc tgccaacaag ccggcaatca gcaccagaaa aatcaagatg
    19681 cactgtttaa cggcaaagcg gtgtaccaat ggcaacttaa aaatgccgag agttatctgc
    19741 tggataacga caaagtaatt tttggggttg cggacggcgt ttccagccac cctcgaaatc
    19801 atcttgcctc acattattta atggcgttac tcagccaatg ccaatccctt gatagccaat
    19861 ggttgcgaca ggctcacggc gatttttgcg aaaaattagc gaaagattat tttggtgctt
    19921 cttgcacttt tgttgcttgt gaattacacc ataacggcag agggcgtatg gttaatgtgg
    19981 gcgacagtcg agcctataaa attacggcaa acggggagtg gcaacaactg agttttgatc
    20041 acaccatact ggctgaaatg aaacagaaag gcttggttaa ggaagggagc aactatgcct
    20101 caatgtataa cggcttaacc gattgtattg ttgccgattt tgacgccgat gatttccgca
    20161 ttcatatagc cgaatttcaa ctggaaaaag gcgagagcat tttgctctgt tctgacggtt
    20221 tgcacgaaca actcgcccca gaagctattc aggcgattta ggacggatac gaaagcccgt
    20281 ctcatcgtct gcaaatttgc agaaaaatgg taaaaatgca ccgcttgtat gacgattttt
    20341 ctgcggtgat ttgtgaaatt gtgtaattaa aacgacatta tttgtcgcat tggtgcttaa
    20401 aatccgcata aaacagagga gaaaaaaatg aaaaacgatt tccattatgc cgctgaatta
    20461 atccgcaatg cagacggcat tttaattacc gcaggggctg gaatgtcggt ggacagtggc
    20521 ttgcccgatt tccgtagcgt gggagggtta tggaattcct acccaccgct caaaaaattt
    20581 aatttgcaat ttatgcagat tgccacaccg cttgcctatc aggaacgccc tga
//
LOCUS       DANLYI010000021        20175 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.021, whole genome shotgun sequence.
ACCESSION   DANLYI010000021 DANLYI010000000
VERSION     DANLYI010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 20175)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20175)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20175
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.021"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            88..1065
                     /locus_tag="PXD81_000591"
     CDS             88..1065
                     /locus_tag="PXD81_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HDL5536651.1"
                     /translation="MSRNLFQKRLNIKPYEYPELLEFKDAIRHSYWLHTEFNFTGDIQ
                     DYRTTINDHERHVLTRAMLAISQVEVNVKRFWGELYCYFPKPEMDDVGGTFAESEVRH
                     KDAYSFLLEKLGLNEMFSQIHEIEPLMNRIRYMESFMKDKDAGKGEFVLSLVLFSLFV
                     EHISLFGQFIIMMSFNKHKNLFKGISNAVEATSKEEEIHGKFGIALYEILRDEHSELF
                     TPEFFEDLQTLANQAFEAERGILDWIFEEGDLSFITKATVENYIKNRYNNSLSILGLE
                     PPHDIDPELLKETEWFDIEILSTKETDFFNKRSTDYSKKMKQITADDLF"
     gene            1052..2719
                     /locus_tag="PXD81_000592"
     CDS             1052..2719
                     /locus_tag="PXD81_000592"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HDL5536652.1"
                     /translation="MTYFNTDRPDFHWLNEDSRLFLQRGYLLEGTTALDRIRFIAEHA
                     ERKLGIEGYADKFYHYMARGYFSLSSPIWSNFGLDRGLPISCFGSYIGDSIHEIMVTT
                     AEVGMMSKIGGGTSAYFGDIRPRGSLIKNNGKSDGSFNFSKLFDTVIDVISQGTSRKG
                     QFAGYIDIEHGDIDEWLDIHTEGNPIQLMYYGVCVGHDWLESMKAGDPYKRQLWAKLL
                     QRKTETGIPYLFFKDNANAGRPDVYKDKNMTIHASNLCTEIMLPSSNEESFVCCLSSM
                     NLLYFDEWKDTDAPETLTYFLDVVMSEFIEKSQSIPFLDRANRFARRHRALGLGVLGW
                     HSYLQANNIAFDSFQAMQKNNEIFKTLQAKTLKASQELAQRFGEPEILEGYGRRNTTL
                     MSIAPTKSSSFILGSVSPSVEPFKSNYYVKDLAKIKTVYKNPFLEKLLQEKGLDTEEI
                     WTSILQNDGSVQHLEQLSEHEKEVFKTFAEISQLSVIQQAAQRQKYIDQGQSINVMIH
                     PATPARDLNQLYLTAEELGLKSIYYQNSMSAAQVFNRNLLSCSSCEG"
     gene            2723..3142
                     /locus_tag="PXD81_000593"
     CDS             2723..3142
                     /locus_tag="PXD81_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5536653.1"
                     /translation="MGNLSHTSPNTIVEVVGEFPEITSSGYTFDKGPIQLRIGKHTAP
                     NKGFGVKHIEAEHMQEILNLGFTSVNEFVKAIIVENAKIFCEFSDLKGNHRPIILKAS
                     IGIVVLERKFLESGTIYSVVTAYRRTNPHGIQIGTMK"
     gene            complement(3377..4138)
                     /gene="nudC"
                     /locus_tag="PXD81_000594"
     CDS             complement(3377..4138)
                     /gene="nudC"
                     /locus_tag="PXD81_000594"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HDL5536654.1"
                     /translation="MNKGYWIIVSDFDIALEAGEIPFGSAEELGLSGREKLTIGQYQG
                     SPAYLVKALESDNQREFAYLRSQINRPPELSHLLHRAVSLNHFFQTHQFCGKCGEKTI
                     LASDEIAIYCNACGNRAYPTISPSIIVAVRKGRQILLANHLRHKGTIYTTLAGFVEAG
                     EAVETAVHREVFEETGIKVKNVRYFGSQPWAFPNSLMLGFLADYESGEIQLQEEEICD
                     AKWFDCEESLPEIPQQGTIAFELIQATLKLCRESK"
     gene            complement(4131..4814)
                     /locus_tag="PXD81_000595"
     CDS             complement(4131..4814)
                     /locus_tag="PXD81_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HDL5536655.1"
                     /translation="MKQPTFKRMTNKRSNFAKNLQKNPQKRPLTFAETTLVLLNKPYD
                     VLTQFTDEQGRKTLKDFITMPNIYPVGRLDRDSEGLLLLTNNGEIQHRLAHPKFDKQK
                     TYWVQVEGVPQPEDLAKLAKGVELKDGLTKPAKVRAIATPDFAWNIAPQIRERKTIPT
                     SWIELIISEGKNRQVRRMTAHIGFPTLRLIRVGIGQFRLGNLGCGEFQVLSENEKRKL
                     FTQLGLKDE"
     gene            complement(4811..5659)
                     /locus_tag="PXD81_000596"
     CDS             complement(4811..5659)
                     /locus_tag="PXD81_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005595732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDL5536656.1"
                     /translation="MEKRTLALLKVHGTAVLFGASGIFGALIQSSAETIVLGRVMIAF
                     AIISLYFLWKKQPLVKLNLQQMAQLAFSAALLAAHWVTFFVAVKVGGVAVATLGFASF
                     PAFVALFESLFFREKLRFREVVLLIAITVGLILVTPEFTFGNQATQGLLWGVLSALVY
                     GILAVANRRTMSKISGVQASWWQYIFATLFLLPLSITEFPSVSTQDWFWIFCIGFLCT
                     TLAYTLFISSLDTINARTASMIISLEPVYAIVIAWLCFNEVPTLKMIIGGLIILLSVA
                     WANLKK"
     gene            complement(5659..6144)
                     /locus_tag="PXD81_000597"
     CDS             complement(5659..6144)
                     /locus_tag="PXD81_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxsA family protein"
                     /protein_id="HDL5536657.1"
                     /translation="MPILAILFGIFFYIYCEISLLVNIGSIIGVLPTILLLVVISFLG
                     LWIVKLRGVYTLFSIKQDLSQGKIPTEAVGNSVMFIVAGILLIIPGFLSDILAILCVL
                     PFSRKWIQAFVINSVKNKVFMHFTAQTSSFSVTSAQDSNTFDAEFERKPDEQDQHNRI
                     K"
     gene            complement(6146..7468)
                     /gene="pepQ"
                     /locus_tag="PXD81_000598"
     CDS             complement(6146..7468)
                     /gene="pepQ"
                     /locus_tag="PXD81_000598"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HDL5536658.1"
                     /translation="MKNLFSTHLARLQQTVQSLLENAFLDGLWIYAGKAKTLFLDDQT
                     APFKINPYFNYFFPYPQAENCWLFLDGKNKPKIYFYAPEDYWVSTLKVPTEAFFAAEF
                     EWVLLRAENEIATFIQNPTACAFIGEDVELAKKLGFSHINPQKVLNSLNFARSIKTEF
                     EIESIYQAQFSALKGHQVAKEAFFAGKSEFEINLEYLKATQQSDLNVPYGNIVAINQN
                     GAVLHYTALDFDTHAKRHSFLIDAGTTVNGYASDLTRTYAFDSGSEFAELVAKMEQLK
                     LQIIAELSIGVNYLHYHTRMQQMISQLLAEHQFIHLSAEQIFEQGISRTFFPHGLGHL
                     LGIQVHDVGGFQQNARGSHKAPPEVYPSLRCTRELQENMVLTIEPGFYFIEMLLKPWK
                     NSALSSAFNWQKIEAFKAFGGIRTEDNIVMRANGAENLTANAAATLGG"
     gene            7479..7721
                     /gene="tusA"
                     /locus_tag="PXD81_000599"
     CDS             7479..7721
                     /gene="tusA"
                     /locus_tag="PXD81_000599"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005710491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HDL5536659.1"
                     /translation="MTQLTVNKELDTLGLRCPEPVMLTRKTIRNMEEGEILHIIADDP
                     ATTRDIPSFCEFMDHQLIQKEVDSTPYHYWIKKGLE"
     gene            complement(7788..8219)
                     /gene="rraB"
                     /locus_tag="PXD81_000600"
     CDS             complement(7788..8219)
                     /gene="rraB"
                     /locus_tag="PXD81_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HDL5536660.1"
                     /translation="MYNLDELRTETDETISSLLADGSDPEALYIIEHHVAHHDFDKLE
                     KLVVDAYKLGYEISEAEEVEDENGKVIFVCDIVSEIHLNADLITAQQKELLPLIEKAG
                     AEYEGWGTYFEDPNADEDEYGDDGEFFDDENDDFEDEPRRH"
     gene            complement(8234..8845)
                     /locus_tag="PXD81_000601"
     CDS             complement(8234..8845)
                     /locus_tag="PXD81_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HDL5536661.1"
                     /translation="MKLNYHKTHFLTSAPDIRHLPEDTGVEIAFAGRSNAGKSTALNA
                     LTNQKSLARTSKTPGRTQLINLFKVETNCKLVDLPGYGYAAVPEQMKIQWQKALGEYL
                     QKRECLGGVVILMDIRHPLKDLDQQMIEWAVSANLPVLLLLTKADKLSQSARSKQVKM
                     VREAILPFQGDIQVEAYSALNKIGIDKLSNKLDSWFAPKLITE"
     gene            complement(8847..9263)
                     /locus_tag="PXD81_000602"
     CDS             complement(8847..9263)
                     /locus_tag="PXD81_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HDL5536662.1"
                     /translation="MSWYKALFSFNGRLNRRGFWIGFGIHFLFLFMVAMWINLAELSL
                     LSVLPLIITGFSLSAIIIKRLHDRNRSAKALLMALVPIICYLAAPYSQGIMQILLGQV
                     MPAFIGMLLFLEWGVFAGNPEPNQYGEQGLSFKFRE"
     gene            complement(9267..10097)
                     /locus_tag="PXD81_000603"
     CDS             complement(9267..10097)
                     /locus_tag="PXD81_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005618704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HDL5536663.1"
                     /translation="MFEYFLSLSPIWQAFLAGLFTWGCTIFGSSFVYFFKSVNRKLLD
                     SMMGFAAGVMIAASFWSLLAPSLEYAEADYGSLAWLPAAIGFLAGGAFIRLIDYVVPH
                     LHLSKPIEQAEGNDQLKQGLSKSTLLFLAITIHNIPEGLAIGVAFGALATQVSGVDAS
                     IMGAIGLAIGIGLQNIPEGSSLVLPIRGEGHSRKKAFWYGSMSAVVEPIAAVIGAVFV
                     LSMTAILPYALAFAAGAMIFVVVEELIPESQSSGNTDIATLSLMLGFVVMMVLDVALG
                     "
     gene            complement(10434..10549)
                     /gene="rrf"
                     /locus_tag="PXD81_000604"
     rRNA            complement(10434..10549)
                     /gene="rrf"
                     /locus_tag="PXD81_000604"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(10711..13609)
                     /locus_tag="PXD81_000605"
     rRNA            complement(10711..13609)
                     /locus_tag="PXD81_000605"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            complement(13926..14001)
                     /locus_tag="PXD81_000606"
     tRNA            complement(13926..14001)
                     /locus_tag="PXD81_000606"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13966..13968),aa:Ala,seq:tgc)
     gene            complement(14058..14134)
                     /locus_tag="PXD81_000607"
     tRNA            complement(14058..14134)
                     /locus_tag="PXD81_000607"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(14098..14100),aa:Ile,seq:gat)
     gene            complement(14211..15750)
                     /locus_tag="PXD81_000608"
     rRNA            complement(14211..15750)
                     /locus_tag="PXD81_000608"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            complement(16104..18065)
                     /gene="recD"
                     /locus_tag="PXD81_000609"
     CDS             complement(16104..18065)
                     /gene="recD"
                     /locus_tag="PXD81_000609"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HDL5536664.1"
                     /translation="MLNLLLELKQEKAISDLNYQFARFIDNKQKPYGYSPLEQDLAVL
                     AAALVSYQVGLGHTALRLENLSLSSLFELNSKPQTYHLLEQILQKIEQIPPLEWQSVL
                     KNHIAFSYSPETIKPMLFQNSLLYFYRYWQAEHRIAGYLQQAVDFSEKFANTELNQSI
                     LNQLFTGKAQTIDWQKIAVATALEKQFSLISGGPGTGKTTTVVKLLLGLQLKQQALQK
                     PYLQIALAAPTGKAAARMKESLESKLAEEKGLSEELKRSIPTEAVTIHRLLGARPLTE
                     KTKYHTKNPWHYDLVVLDEASMIDLSMMEKVVQALKPNSRLIMLGDKDQLASVEAGSI
                     MGELGSFIDNGYSQSHCDYLKAVTDYQIEAGNALPIGDCLSHLKHSYRFGEKAWIGEL
                     ANAVNDQKINLSWEIFDKYKNSDKLENFCYPETKETTDKSSWIEKSVQMVVDKAVELY
                     RDYLQAVQKREKNPDSVSVKAIFEKFQKVRILSALRVSELGVERLNMAIAEGLRKASL
                     VKFNHSRDRYIGKPILMTENMPINSIASGDVGIILPDENGESRVYFDSLQENREHHSL
                     PLGRVSNYEAAYIMTVHKSQGSEFEHTVLVLPHSISPVLTKELIYTAITRAKDKFTLF
                     GSEKVWKYSVGAKTERQSGLKEQIRGKFS"
     gene            18195..19319
                     /gene="alr"
                     /locus_tag="PXD81_000610"
     CDS             18195..19319
                     /gene="alr"
                     /locus_tag="PXD81_000610"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011848339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HDL5536665.1"
                     /translation="MKPATATIDSQALRHNIRLIKSFAPQQKLLAMIKANAYGQGLLP
                     AAGTLADMVDGFGVARLREALEIQETGYTGKIVLVEGFFDREELLKTLSRKFDTVIHN
                     LEQLELLEQVAKEWQAEQQKGFWTRKAKIYFPINIWLKIDTGMHRLGIHPEQVDHFVE
                     RLKNCALVNSISFVSHFSRADELDCGYTEKQIAVFEQATQKYQDHDRSISASSGILYW
                     KQAHYDWVRPGIIMHGISPHYTPITDLGFKPVMTLSSSLIAVRTHKAGEPVGYGGAWV
                     SPKDTKLGVTAMGYGDGYPRNAPEGTPVLINGRIVPIVGRVSMDMLTVDLGAESQDKV
                     GDKAIFWGEQLLIEDVAKHIGVISYELITKLTPRVIFEYK"
     gene            complement(19382..20023)
                     /gene="sodA"
                     /locus_tag="PXD81_000611"
     CDS             complement(19382..20023)
                     /gene="sodA"
                     /locus_tag="PXD81_000611"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005708524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HDL5536666.1"
                     /translation="MAYTLPELGYAYDALEPHFDAKTMEIHHSKHHQAYINNANAALE
                     AHPELLEKCPGALIKDLSQVPAEKRIAVRNNVGGHVNHTLFWKGLKTGTTLQGALKEA
                     IERDFGSVEAFQSEFEKAATTRVGSGWAWLVLEEGKLAVVSTANQDNPLMGKEVAGVS
                     GYPILVLDVWEHAYYLNYQNRRPDFIKAFWNVVNWDEAARRFEEKVATCGCAK"
BASE COUNT         5705 a         4752 c         3872 g         5846 t
ORIGIN      
        1 gtgtttgtca agacttgtct tgaaactttt tttgcataca atcatctacc agttccaaac
       61 ataaacttta agatagagcc tacgaatatg tctagaaacc tcttccaaaa acgcttaaat
      121 attaagccgt atgagtatcc tgagcttctt gaatttaaag atgcaattcg ccattcttac
      181 tggttacaca ccgaatttaa cttcaccggc gatattcaag actatcgcac caccattaac
      241 gatcacgaac gtcacgtgtt aacccgagca atgcttgcga tttcacaagt agaagtgaat
      301 gtaaaacgtt tctggggcga gctatactgc tatttcccaa aaccggagat ggacgatgta
      361 ggcggtactt ttgctgaaag tgaagttcgc cataaagatg cttactcgtt cctattggaa
      421 aaattaggcc taaatgaaat gttcagtcaa attcatgaaa ttgaaccatt aatgaaccgc
      481 attcgttata tggaaagttt tatgaaagat aaagatgctg gcaaaggcga atttgtttta
      541 tcgttagtgc tgttctcttt atttgttgag catatttcat tgtttggaca gtttattatc
      601 atgatgtcgt tcaacaagca taaaaacctg tttaaaggga tttccaatgc ggttgaagcc
      661 acctcaaaag aggaagaaat tcacggtaaa ttcggaattg cgttatacga aattttacgt
      721 gacgaacaca gtgaactatt cacaccagaa ttttttgaag atttgcaaac cttggcaaat
      781 caagcgtttg aagcagaacg tggcatctta gactggattt tcgaagaagg tgatttaagc
      841 tttatcacca aagcgacggt agaaaattac attaaaaacc gttacaacaa ttctttaagc
      901 attttagggt tagagccgcc acacgacatc gaccctgaat tattaaaaga gactgagtgg
      961 tttgatattg aaattctttc aaccaaagaa accgatttct ttaacaaacg tagcaccgat
     1021 tattctaaaa aaatgaaaca gattaccgca gatgacttat tttaatacag accgcccgga
     1081 cttccactgg cttaatgaag acagccgttt attcttacaa cgtggctatt tattggaagg
     1141 aacaaccgca ttagaccgca ttcgttttat tgccgaacac gcagagcgaa aactcggcat
     1201 tgaaggctat gccgataaat tctatcacta tatggcaaga ggctacttct cgctttcttc
     1261 gccaatttgg tcaaactttg gcttagatcg tggcttaccg atttcttgtt tcggtagcta
     1321 catcggcgac tctatccatg aaattatggt gactaccgct gaagtgggca tgatgagtaa
     1381 aattggcggt ggtacttcgg cctactttgg cgatattcgt ccgcgtggct cgttaattaa
     1441 aaacaacggt aaatcagacg gctcatttaa cttcagtaaa ttatttgaca ctgtgattga
     1501 cgtgatttca caaggaacat cacgcaaagg tcagtttgcc ggttatattg atattgagca
     1561 cggcgatatt gacgaatggc tggatattca caccgaaggc aacccaattc aattaatgta
     1621 ttacggtgtg tgtgtcggtc acgattggtt agagtcaatg aaagccggcg atccatacaa
     1681 acgtcagctt tgggcaaaat tgttacaacg taaaaccgaa accggtatcc cttatttatt
     1741 ctttaaagac aatgccaacg caggtcgtcc tgatgtatat aaagataaaa atatgacgat
     1801 tcacgcctct aacttatgta ctgaaattat gttgccttcc agcaacgaag aaagtttcgt
     1861 atgctgttta tcgtctatga acctactcta ctttgatgag tggaaagata ccgatgcacc
     1921 agaaacatta acctatttcc tcgatgttgt aatgagtgaa tttattgaga aaagtcaatc
     1981 aattccattc ttagaccgtg caaaccgctt tgcccgtcgc catcgtgcat taggtttagg
     2041 cgtgttgggc tggcacagct atctacaggc aaataatatt gcctttgaca gcttccaagc
     2101 gatgcaaaaa aacaacgaga ttttcaaaac gcttcaagca aaaaccttga aagcctcgca
     2161 agaattggct caacgcttcg gcgagccgga aattttagag ggctatggtc gccgtaatac
     2221 tacattaatg agtattgctc cgactaaatc cagtagcttt attttaggca gtgtgtcgcc
     2281 atcggtagag ccgtttaaat ccaactacta tgtaaaagat ttagcgaaga tcaaaaccgt
     2341 gtataaaaac ccgttcttag agaaattact ccaagaaaaa gggctagaca ccgaagaaat
     2401 ttggacgtct attttacaaa atgatggctc ggttcagcat ttggaacagc tttccgagca
     2461 cgaaaaagaa gtatttaaaa cctttgccga aatcagccaa ttaagcgtga ttcagcaagc
     2521 tgctcaacgt caaaaatata ttgaccaagg gcaaagtatt aatgtgatga tccacccagc
     2581 cacaccggct cgtgatctta accaattata cttaacagca gaagagctgg gcttaaaatc
     2641 catctactac caaaactcaa tgagtgcagc acaggtattt aaccgtaacc tattaagctg
     2701 ctcaagttgc gaagggtaaa taatgggaaa tttatcgcat acttcaccta atactattgt
     2761 tgaagttgtt ggtgaatttc ctgaaataac atctagtggg tatacctttg ataaaggtcc
     2821 tattcaatta agaatcggca aacatactgc tccaaataaa ggctttggtg taaagcatat
     2881 tgaagccgag catatgcaag aaattcttaa tctaggattt acatcagtta atgaatttgt
     2941 caaagctatc atagtagaaa acgctaaaat tttttgcgaa ttctcagatt taaaaggcaa
     3001 tcatcgccct attattctga aagcaagtat tggcattgtg gttctagagc gtaagttttt
     3061 ggaaagcggt acgatatatt cagttgtaac cgcttataga agaacaaatc ctcacggaat
     3121 acaaataggc actatgaaat aaagtacgtt agtgttataa tcagttccac gcaagagcat
     3181 ttgtagaaaa taacaggcgg atttggtttt ctactgtggg cggcaggttt ccgctaacct
     3241 gtaatagcta tttaattttt atattaaatc ctgaacttga aacagactaa gggattgctg
     3301 cggtagctaa gaatacaaac ctctctataa tcatatagag aggttttttc ttattgatga
     3361 gcacaagtta agataattat ttactctccc gacataactt caaggtagct tgaatcagct
     3421 caaaagctat tgtgccttgt tgtgggattt caggtaagct ttcctcacag tcgaaccatt
     3481 ttgcatcgca gatttcttct tcctgtagct gaatttcacc gctttcataa tccgctaaaa
     3541 aacctagcat taatgaattg ggaaacgccc aaggttggct tccaaaataa cgcacatttt
     3601 tcactttaat gcccgtttct tcaaacactt cacgatgcac cgcagtttct accgcctcac
     3661 cggcttcgac aaaaccggct aaagtggtat aaattgtgcc tttatggcgt agatgattgg
     3721 ctagtaggat ttgtctgcct tttcgcaccg ccacaataat tgacgggcta atcgttgggt
     3781 aagctcgatt gccacacgca ttgcagtaaa ttgcaatttc atcgcttgcc agtatggttt
     3841 tctcgccaca ttttccgcaa aattggtgag tctgaaagaa atggttaagc gaaacagctc
     3901 gatgcaacaa atgagacagt tcgggtggtc ggttaatttg gcttcttaaa taagcaaact
     3961 ctcgctgatt atccgattct aacgccttca ccaaataagc cggcgagcct tgatattgcc
     4021 cgatggttaa tttctcacgt ccacttaagc ccagctcttc cgcacttccg aatggaattt
     4081 ccccagcctc caaggcaata tcaaaatcgc tgacgataat ccaatatcct ttattcatct
     4141 ttcaatccta attgtgtaaa caacttacgt ttttcatttt cagatagcac ttgaaactca
     4201 ccacagccta aattgcctaa tctaaactgg cctatcccaa cacgaattaa ccgtaaagtc
     4261 gggaaaccga tatgtgcagt cattcttctg acttggcgat ttttcccttc agaaataatc
     4321 aattctatcc agctggtcgg aatggttttc cgctcacgaa tttgcggggc gatattccaa
     4381 gcaaaatcgg gcgttgctat tgctctgact ttcgcgggct tggtcaaacc atcttttaac
     4441 tccacccctt ttgctaattt ggcaagatct tccggttgtg gaacaccctc cacttgtacc
     4501 caataggttt tctgcttgtc aaatttcggg tgagctaaac ggtgctggat ttcgccatta
     4561 ttagtcaaaa gcaacaatcc ttcactgtct ctatctagcc gcccgaccgg gtaaatattg
     4621 ggcatcgtaa taaaatcttt tagcgttttt cgcccctgtt catcggtaaa ttgggttagt
     4681 acatcataag gcttattgag taacacaagg gtggtttccg caaaggtaag cggtcgtttt
     4741 tgcggatttt tttgcaaatt tttagcgaaa tttgaccgct tgtttgtcat tcttttaaag
     4801 gttggttgtt tcattttttc aggttcgccc acgcaacaga aagtaaaata attaagccac
     4861 cgataatcat tttaagggtt ggcacttcat taaagcaaag ccacgcgatc acaatcgcat
     4921 acaccggctc gagggaaata atcattgagg cagtacgggc gttgatggta tcaaggcttg
     4981 aaataaacag cgtataggca agggttgtac ataaaaaacc gatacagaaa atccaaaacc
     5041 aatcttgggt ggaaacgctt ggaaattcag tgatggagag aggcaacaga aaaagtgtag
     5101 caaaaatata ctgccaccag cttgcttgca caccggaaat tttgctcatg gtacggcgat
     5161 tcgctaccgc caaaatacca tacaccaaag cagataacac gccccacaat aaaccttgag
     5221 tggcttgatt accaaaagta aattcagggg taactaaaat taaaccaacc gtgatggcaa
     5281 ttaatagcac cacttcacga aagcgtaatt tttcacggaa gaacaggctt tcaaataacg
     5341 ccacaaatgc cggaaagctg gcaaagccta aggttgctac cgccacacca cctactttaa
     5401 ccgccacaaa aaaagtaacc cagtgggcag ctaataaagc tgcagaaaat gcaagttgag
     5461 ccatttgctg taagttgagc tttacaagcg gttgtttttt ccataaaaaa tacaaagaga
     5521 tgatagcaaa agcaatcatc actcgcccaa gcacaatggt ttcagccgag ctttgaatta
     5581 atgctccgaa gatccccgag gcaccaaata aaaccgctgt gccgtgtact ttaagtagtg
     5641 caagtgttct tttctccact atttgatcct attatgttga tcttgctcgt ccggcttgcg
     5701 ttcaaattct gcatcaaacg tgttgctatc ttgcgcagat gtaacagaaa aagacgaagt
     5761 ttgggcggta aaatgcataa acaccttatt ctttacgcta ttaattacga aggcttgaat
     5821 ccattttcgg ctaaagggta acacgcataa aatcgctaaa atatcactta aaaagccggg
     5881 aataatcagc aaaatgccgg ctacaataaa catcaccgag ttgccgactg cttcagtcgg
     5941 tatcttgcct tggcttaaat cttgcttgat ggaaaagagg gtataaacgc cccttaactt
     6001 cacaatccat aaccctaaaa atgaaatcac gaccagtaac aaaatcgtgg gtaacacgcc
     6061 aatgatcgag ccaatattca ccagcagcga aatttcgcaa tagatataaa agaaaatacc
     6121 aaacagaatc gctaaaatag gcatattatc ctcccaacgt tgcggcagcg tttgccgtca
     6181 gattttctgc cccattggct cgcatgacaa tattgtcttc ggtacgaatc ccgccaaagg
     6241 ctttgaaagc ctcaattttc tgccagttaa aggcagaaga taaggcactg tttttccaag
     6301 gcttaagtag catctcaata aaataaaaac ccggctcaat ggtaagcacc atattttcct
     6361 gtagctcacg ggtacaacgc aaacttgggt aaacctctgg aggagcttta tggctgcctc
     6421 gggcattttg ctgaaaaccg cccacatcgt gcacttgaat acccaataaa tgccctagtc
     6481 cgtgtgggaa gaacgtacgg ctaatccctt gttcaaaaat ttgctcggct gaaagatgaa
     6541 taaattgatg ttccgctaat aattgcgaaa tcatctgctg cattcgggtg tgataatgca
     6601 ggtagttcac cccaatactc agctcagcaa taatctgcaa cttcaactgt tccattttcg
     6661 ccaccagctc ggcaaattca gaaccgctgt caaaggcata ggtgcgggtg agatcggagg
     6721 cataaccatt taccgttgtg ccggcatcaa ttaaaaaact gtggcgtttt gcgtgggtgt
     6781 caaaatctaa cgccgtataa tgtagcaccg caccattttg attaatcgcc acaatattgc
     6841 cataaggaac attgagatca gattgctgcg tcgctttcaa atattcaagg ttgatttcaa
     6901 attcgctttt gccggcaaaa aatgcctctt ttgctacttg atgccctttt aaggctgaaa
     6961 attgggcttg gtaaatactt tcaatttcaa actccgtttt gatggagcgg gcaaaattca
     7021 ggctattcag cactttctgc ggattaatat ggctaaagcc taactttttg gctagctcaa
     7081 catcttcacc gataaaggca caagcggtcg gattttggat aaaggttgca atttcattct
     7141 ctgctctcaa caaaacccac tcaaattccg cagcgaaaaa ggcctccgtt ggcactttta
     7201 gagtggaaac ccaataatct tcaggggcat aaaaatagat tttcggctta tttttgccat
     7261 ctaaaaataa ccagcaattt tcagcttggg gatatgggaa aaagtaatta aaataagggt
     7321 taattttaaa cggggcggtt tgatcgtcta aaaataaggt ttttgccttg ccggcataaa
     7381 tccataaacc gtctaaaaag gcattttcca gcaagctttg cactgtctgt tgcaaacgtg
     7441 cgagatgcgt agagaataga tttttcataa ggaataaaat gactcaatta actgtaaata
     7501 aagaattaga taccttgggg ctacgttgcc cagagccggt gatgctcacc cgtaaaacaa
     7561 tccgtaatat ggaagaggga gaaattttgc atatcatcgc cgatgacccg gccactaccc
     7621 gtgatattcc gagtttctgc gaatttatgg atcaccaatt aattcaaaaa gaggtggact
     7681 ccaccccata ccattactgg attaaaaaag gcttggaata aaccacaagc ggtcgatttt
     7741 agccataaaa ttgcaaattt atgcacaaaa tcgaccgctt gttttaatta atgacggcga
     7801 ggttcgtcct caaaatcgtc attttcatca tcgaaaaatt caccgtcatc gccgtactca
     7861 tcttcatcag cattcggatc ttcaaagtag gttccccagc cctcatactc cgcaccggct
     7921 ttttcaatta atggcagtaa ctctttttgt tgtgcggtaa tgagatccgc attcaagtga
     7981 atttcgctca caatgtcgca tacaaaaatc actttaccat tttcgtcttc cacttcttcc
     8041 gcttcggaaa tttcatagcc cagcttatag gcatcaacta ctaatttttc caatttatca
     8101 aaatcgtggt gagcaacgtg gtgttcaata atataaaggg cttcagggtc tgaaccatcg
     8161 gctaataatg atgaaatggt ttcatcggtt tctgtgcgta attcatcgag attgtacata
     8221 ttgaattcct tacttattct gtgattaatt ttggtgcaaa ccagctatct aatttgttcg
     8281 acagcttatc aataccgatt ttattcagtg cggaataagc ctctacttgg atatcacctt
     8341 ggaatggtaa aatcgcttct cttaccattt ttacctgctt agagcgtgcc gattggctta
     8401 acttatccgc tttggtcaac agcagtaaca ccggcaagtt ggctgacact gcccattcaa
     8461 tcatctgttg atctaaatct ttcaacgggt ggcgaatatc cattaaaatc accacaccgc
     8521 ctaaacattc acgcttttgt aaatactcgc ctaatgcttt ttgccactgg attttcatct
     8581 gttcaggtac tgctgcatag ccatagccag gtaaatccac taatttacaa ttcgtttcaa
     8641 ccttaaacaa gttaatcaac tgcgttcgcc ccggtgtttt ggaagttcgg gctaaacttt
     8701 tttggttagt taaagcgttc aaagcagtcg atttaccggc atttgaacgc ccggcaaaag
     8761 caatctccac acctgtatct tcaggtaaat gacgaatatc cggtgctgaa gttaaaaaat
     8821 gggttttatg atagtttaat ttcattttat tctctaaatt taaaagataa gccttgctcg
     8881 ccatattgat tcggttcagg attaccggca aatacacccc attctaaaaa gagcaacatt
     8941 ccaataaatg ccggcatcac ctgtccaagc aatatctgca taattccttg tgaataagga
     9001 gctgcaagat aacaaataat cggcactaat gccattaaca gggcttttgc cgaacgattt
     9061 cggtcgtgta atcgtttaat aataatagca gacaggctaa atcctgtaat tataagcggt
     9121 agcacactca ataaagacaa ttcagccaaa tttatccaca tagcaaccat aaacaggaaa
     9181 agaaagtgga ttccaaatcc aatccaaaaa cctcgtctat ttagcctacc gttaaaacta
     9241 aaaagtgctt tataccaact cattttctac cctaaagcta catcaagtac catcattact
     9301 acaaagccta acatcaggct taaagtcgca atatcagtat ttcccgaact ttgcgattcc
     9361 ggaatcagtt cttctacgac cacaaaaatc atcgctccgg cagcaaaggc aagagcataa
     9421 ggtaaaatag ccgtcatcga cagcacaaaa accgccccaa tcaccgccgc aataggctca
     9481 accactgctg acatagagcc ataccaaaat gctttcttcc gactatgccc ttcgcctcga
     9541 attggcaata caagtgacga gccttcggga atgttttgta accctattcc gattgccaaa
     9601 ccgatagctc ccataatgga agcatctacg cctgaaactt gggttgccaa agcaccgaaa
     9661 gccaccccta tggctaatcc ttctggaata ttatgaatgg taatcgcaag aaaaagcaaa
     9721 gtagattttg ataagccttg tttaagttga tcatttcctt cagcttgctc aatcggctta
     9781 ctcaagtgca aatgcggcac aacatagtca attaatcgaa taaaagctcc accggcaaga
     9841 aagccaatcg ctgcaggcag ccaagctaaa gagccataat ccgcctcggc atattctaaa
     9901 gaaggtgcaa gcaaagacca aaacgaggct gcaatcatta cacctgcggc aaagcccatc
     9961 atactgtcta ataattttcg attaaccgat ttaaaaaaat agacaaatga cgaaccgaaa
    10021 atcgtacaac cccaagtaaa taaacccgct aaaaaagctt gccatattgg agaaagtgaa
    10081 aggaaatatt caaacaagaa atagtaccag ctaaaataaa tattggctca ttctatcaaa
    10141 gattcagggt tattaatata gtgtagccaa aggctcaacg aagatttcac ttgtaaaact
    10201 tagcaagaaa ataaccgctt gcatttccct ttcataaata caatgaaaat cctaacaagc
    10261 ggtcattttc cgtgagtttt ttacctttac cacaacaaca aaaaaccgcc tgtatctcta
    10321 caggcggctc tttcttggat tacttgtact ttgtttattt gtacttattt tgcttttgct
    10381 taagttttcg cttttgtaac ttttagcttt cgcttaagct cgcttaaatt aaaacccgac
    10441 gatgctctac tctcacatgg cgaatcacca cactaccatc gacgttactg cgttttactt
    10501 ctgagttcgg tatggggtca ggtaggacca cagcactctt gtcgtcggga aaattctttt
    10561 ctatttttga atttctttaa gttaacttcc taatctaact cttagttcac tttaatagtt
    10621 agattaactt ccttaacttc gattgatttc ttctctagtt tcgttcttct tctcaataca
    10681 aaaacaagcc taaaaacatt tgagcgttgt atagttaagc ctctcgggca attagtactg
    10741 gttagctcaa tgtctcacaa cacttacaca cccagcctat ctacgtcgta gtctccaaca
    10801 acccttacac acttatagtg tgggagaact catctcgagg caagtttcgt gcttagatgc
    10861 tttcagcact tatctcttcc gcatgtagct acccagcaat gcctctggcg agacaactgg
    10921 aacaccagtg atgcgtccac tccggtcctc tcgtactagg agcagcccct ctcaattctc
    10981 caacgcccac ggcagatagg gaccgaactg tctcacgacg ttctaaaccc agctcgcgta
    11041 ccactttaaa tggcgaacag ccataccctt gggacctact tcagccccag gatgtgatga
    11101 gccgacatcg aggtgccaaa caccgccgtc gatatgaact cttgggcggt atcagcctgt
    11161 tatccccgga gtacctttta tccgttgagc gatggccctt ccattcagaa ccaccggatc
    11221 actatgacct gctttcgcac ctgctcgact tgtctgtctc gcagttaagc ttgcttatac
    11281 cattgcacta acctcacgat gtccgaccgt gattagcaaa ccttcgtgct cctccgttac
    11341 tctttgggag gagaccgccc cagtcaaact acccaccaga cactgtccga gaccgcgttc
    11401 cgcaatcttc gttagaacat caaacgttaa agggtggtat ttcaaggacg cctccacaat
    11461 cactggcgtg actgcttcaa aggctcccac ctatcctaca catcaaaatt caatgttcag
    11521 tgtcaagcta tagtaaaggt tcacggggtc tttccgtcta gccgcgggta caccgcatct
    11581 tcacggcgat ttcaatttca ctgagtctcg ggtggagaca gcctggccat cattatgcca
    11641 ttcgtgcagg tcggaactta cccgacaagg aatttcgcta ccttaggacc gttatagtta
    11701 cggccgccgt ttactggggc ttcgatcagg agcttctctt gcgataacac catcaattaa
    11761 ccttccagca ccgggcaggc atcacaccct atacgtccac tttcgtgttt gcagagtgct
    11821 gtgtttttaa taaacagttg cagccagctg gtatcttcga ccggttcaac cttcatccgc
    11881 aagggactac aatctacgcc ggcgcacctt ctcccgaagt tacggtgcta ttttgcctag
    11941 ttccttcacc cgagttctct caagcgcctg agtattctct acctgaccac ctgtgtcggt
    12001 ttatagtacg gtttataata acctgaagct tagtggcttt tcctggaagc gtggtatcga
    12061 ttacttcatc tccgtagaga ctcgtcatca cttctcggtg ttaaggaaga ccggatttgc
    12121 ctaatcttcc cacctaccgg cttaaacaga catccaacag tctgctaacc taaccttctc
    12181 cgtccccaca tcgcagttat tacaagtacg ggaatattaa cccgtttccc atcgactacg
    12241 cttttcagcc tcgccttagg ggccgactca ccctgccccg attaacgttg gacaggaacc
    12301 cttggtcttc cggcgaacga gtttttcact cgttttatcg ttacttatgt cagcattcgc
    12361 acttgtgata tctccagcag acttctcaat ccaccttcat cgacttacac aacgctcccc
    12421 tacccaacag actttcgtct gatgccgcag cttcggtgct atatttgagc cccgttacat
    12481 cttccgcgca ggccgactcg actagtgagc tattacgctt tctttaaatg gtggctgctt
    12541 ctaagccaac atcctagctg tctaagcctt cccacttcgt ttcccactta atatagactt
    12601 tgggacctta gctggcggtc tgggttgttt ccctctccac gatggacgtt agcacccacc
    12661 gtgtgtctcc tgagtatcac tcttcggtat tcgcagtttg catcgggttg gtaatccggg
    12721 atggacccct agccgaaaca gtgctctacc cccgaaggtg tccgctcaag gctctaccta
    12781 aatagatttc ggggagaacc agctatctcc cggtttgatt ggcctttcac ccccagccac
    12841 aagtcatccg ctaatttttc aacattagtc ggttcggtcc tccagttagt gttacccaac
    12901 cttcaacctg cccatggcta gatcaccggg tttcgggtct ataccttgca actacacgcc
    12961 cagttaagac tcggtttccc tacggctccc ttattcagtt aaccttgcta caaaatataa
    13021 gtcgctgacc cattatacaa aaggtacgca gtcacaagat tactcttgct cccactgctt
    13081 gtacgcacaa ggtttcaggt tctatttcac tcccctcgcc ggggttcttt tcacctttcc
    13141 ttcacagtac tggttcacta tcggtcaatc aggagtattt agccttggag gatggtcccc
    13201 ccatcttcaa acaggatatc acgtgtcccg ccctacttgt tgttagccta gtaccacaat
    13261 ggagttttaa ggtacgggat tatcaccctc tacgattgtc cttcccagaa cattccccta
    13321 actttactgc tatcactaac tggctctttc gcgttcgctc gccgctacta acgaaatctc
    13381 ggttgatttc ttttcctcgg ggtacttaga tgtttcagtt ctcccggttt gcctcacaca
    13441 gctatgtatt cactgggtga tagtaggttc ttcacctact gggtttcccc attcggacat
    13501 cttggattaa acgcctctta tcgactcatc caagcttttc gcagattagc acgtccttct
    13561 tcgcctctga ttgccaaggc atccaccttg tgcgcttagt cacttaacta tacaacctca
    13621 aatgttttct aaaacttctc aaaaataaaa gtaaaaaaac aatgaagtca gttttacaac
    13681 taaacacttg actgcttttg ttcagtcaag attttttcta ctcagacttt caatttattc
    13741 gttatcactt atgtataatt acttatgcgt aacgaacttg aaatatctct cagtttttca
    13801 gcttgtttcc atattgttaa agaacaaaga taatggcttt cgcctatcat catacctaaa
    13861 attgcaaata atccggtatt tttaaccgct tgtaatctta ggtatgatga taaagtcacg
    13921 atgattggtg gagataagcg ggatcgaacc gctgacctcc tgcgtgcaag gcaggcgctc
    13981 tcccagctga gctatatccc catttcatct tccgttttct tcactcatag tcgcttcgct
    14041 ttcgcttgcc ttctgagtgg tgggtctgag tggacttgaa ccaccgacct cacccttatc
    14101 aggggtgcgc tctaaccacc tgagctacag acccaacgga tgaccttcgg ttttttgtct
    14161 tcttttatca aacaatctgt gtgaacactt gctgtcgttc tcgattttgg taaggaggtg
    14221 atccaaccgc aggttcccct acggttacct tgttacgact tcaccccagt catgaatcat
    14281 accgtggtaa acgcccccct ttcggttaag ctatctactt ctggtacaac ccactcccat
    14341 ggtgtgacgg gcggtgtgta caaggcccgg gaacgtattc accgcaacat tctgatttgc
    14401 gattactagc gattccgact tcatggagtc gagttgcaga ctccaatccg gacttagacg
    14461 tactttctga gattcgctcc ccatcgctgg ctcgccgccc tctgtatacg ccattgtagc
    14521 acgtgtgtag ccctactcgt aagggccatg atgacttgac gtcatcccca ccttcctccg
    14581 gtttatcacc ggcagtctcc tttgagttcc cgacctaatc gctggcaaca aaggataagg
    14641 gttgcgctcg ttgcgggact taacccaaca tttcacaaca cgagctgacg acagccatgc
    14701 agcacctgtc tcatggttcc cgaaggcact ctcatatctc tacaagattc catggatgtc
    14761 aagagtaggt aaggttcttc gcgttgcatc gaattaaacc acatgctcca ccgcttgtgc
    14821 gggcccccgt caattcattt gagttttaac cttgcggccg tactccccag gcggtcgatt
    14881 tatcacgtta gctacgggca ccagagttaa accccaatcc ccaaatcgac agcgtttacg
    14941 gcgtggacta ccagggtatc taatcctgtt tgctccccac gctttcgcac atgagcgtca
    15001 gtacattccc aaggggctgc cttcgccttc ggtattcctc cacatctcta cgcatttcac
    15061 cgctacacgt ggaattctac ccctccctaa agtactctag ttgtccagtc tgaaatgcaa
    15121 ttcccaggtt aagcccgggg ctttcacacc tcacttaaac aaccgcctgc gtgcccttta
    15181 cgcccagtta ttccgattaa cgctcgcacc ccccgtatta ccgcggctgc tggcacggag
    15241 ttagccggtg cttcttctgt gattaacgtc aatcgactgt tctattaaaa caatcgcctt
    15301 cctcgtcacc gaaagaactt tacaacccga aggccttctt cattcacgcg gcatggctgc
    15361 atcagggttc cccccattgt gcaatattcc ccactgctgc ctcccgtagg agtctggacc
    15421 gtgtctcagt tccagtgtgg ctggtcatcc tctcagacca gctagagatc gcgggcttgg
    15481 tgagccttta cctcaccaac tacctaatcc cacttgggct catcttatgg caggtggcca
    15541 aatggtccca ccctttagtc ctaagacatt acgcggtatt agctaccgtt tccagtagtt
    15601 atccccctcc ataagccaga ttcccaagca ttactcaccc gtccgccact cgtcacccaa
    15661 ggagcaaact cctatgtgct accgttcgac ttgcatgtgt taagcctgcc gccagcgttc
    15721 aatctgagcc atgatcaaac tcttcaattc aaaaagttta atcgctcaat actgctgaca
    15781 taaaatcgca cttccaaatt taaacctaat taaaattaag ttaaaagaaa gtaatgaatt
    15841 tctagttagc acctattaag acttcaaaat taaaaaaatg tttttcaatc aagtcaatca
    15901 acaagtgccc acacagattg tctgataaat tgttaaagaa caaaaagaaa cgacgcactg
    15961 gaaatcaatt ttaaatcgtt cacaacagtg cgtcgttgtg tgctgcgtat tatagggaaa
    16021 aacaaaaaca acgcaagcgg tttttgtaaa aaatttgaaa aaaatgaccg cttgcgtatt
    16081 tttacggcaa aacgagtatt aatttagcta aacttgcctc ttatttgttc ttttagccca
    16141 ctttgtcttt cggttttggc accaacactg tatttccaaa ctttttcgct accaaataag
    16201 gtaaatttat cttttgccct tgtgattgcg gtgtaaatca actctttcgt cagcaccggg
    16261 gaaatcgaat ggggcaacac caataccgta tgttcaaatt cagaaccttg cgatttatgc
    16321 acagtcataa tataagctgc ttcataattt gaaaccctac ctagaggcaa actatggtgt
    16381 tctctatttt cctgtaagga atcaaaataa actcggcttt ctccattttc gtccggcaaa
    16441 atgatgccga catcaccgct ggctatgcta tttatcggca tattttctgt cattaaaatc
    16501 ggttttccga tatagcggtc tcggctatga ttaaatttca ctaagctagc tttccgcaag
    16561 ccttccgcaa tcgccatatt aagacgttca acccccagtt cacttaccct taaggctgat
    16621 aaaattcgga ctttttgaaa tttctcaaaa atcgctttca cactcactga atctggattt
    16681 ttctcccgtt tttgaaccgc ttgtaaataa tcacgataaa gttcaaccgc tttatccacc
    16741 accatttgca cacttttttc aatccagctt gatttatccg ttgtctcttt cgtttccgga
    16801 taacagaaat tttctaactt atcactgttt ttatatttgt cgaaaatctc ccacgaaagg
    16861 ttaatttttt gatcatttac tgcatttgca agctctccaa tccaggcttt ttcgccaaat
    16921 cgatagctat gttttaagtg agataaacaa tcaccaatcg gcaacgcatt accggcttca
    16981 atttggtagt cggtaacggc ttttaagtaa tcgcaatggg attggctata accattatca
    17041 ataaaagagc caagttcccc cataattgaa ccagcttcca cagaagcaag ctgatcttta
    17101 tctcccagca ttatcaagcg agagtttggt ttgagcgctt gaacaacttt ctccatcatt
    17161 gataaatcaa tcattgaagc ctcatctaat acgaccaaat catagtgcca cggatttttg
    17221 gtatgatatt tggttttctc agttaatggt ctcgcaccaa gcaagcgatg aatagtcact
    17281 gcttcagttg gaatagagcg ttttaactcc tcagataacc ccttttcttc cgccaacttg
    17341 ctttccagcg actctttcat tcttgctgct gcttttccgg ttggtgcagc cagagcgatt
    17401 tgcaaatatg gtttctgtag tgcttgctgt tttaattgca gcccgagtaa taattttact
    17461 accgtagttg tttttccggt tcccggccca cctgaaatta agctaaactg cttttctaat
    17521 gctgttgcaa cggcaatctt ttgccagtcg atagtttgag ctttcccagt gaatagttgg
    17581 ttgagaatgc tttggtttaa ttcggtattt gcaaattttt ccgaaaaatc aaccgcttgt
    17641 tgtaaataac cggcaatgcg atgctccgct tgccagtagc gataaaaata gagcaagctg
    17701 ttttggaata acatcggttt aattgtttcg ggagaatagc taaaagcgat atgatttttc
    17761 agcacactct gccattcaag cggtggaatt tgctcaattt tttgcaaaat ttgttcgagc
    17821 aaatgataag tttgtggctt gctatttaat tcaaaaagag aagaaagact gagattttcc
    17881 aaacgtaatg ccgtatgccc aagtcccact tggtaagaaa ccaacgcagc ggctaacaca
    17941 gctaaatctt gctccaaagg cgaataccca tagggtttct gcttattatc aataaaacgg
    18001 gcaaattgat aatttaagtc agaaatcgcc ttttcttgtt taagttccaa taataaattc
    18061 agcataatca aaccttaaat cgaccaccta acattaagtg ataatactaa tcttttttgc
    18121 attattttct aagggatttt ttattttagg cgtataattt agcctttctt gtgattttaa
    18181 ctatgtagat aagtatgaaa ccagcaacag caaccattga tagtcaagcc ctccgccata
    18241 atattcgcct gatcaaatct ttcgctccac agcaaaaatt attggcgatg atcaaagcca
    18301 atgcttacgg gcaaggctta ttgccggctg ccggcacatt agcagatatg gttgatggct
    18361 ttggagttgc ccgtttgcgt gaagccttag aaattcaaga aaccggatac acaggcaaaa
    18421 ttgtgttagt tgaaggtttt tttgaccgag aagagctact caaaaccctt tcccgcaagt
    18481 ttgataccgt cattcataac ctcgaacaac tggaattact cgagcaagta gcaaaagaat
    18541 ggcaggcaga gcaacaaaaa ggcttttgga ctcgtaaagc gaaaatctac ttcccgatta
    18601 acatttggct aaaaatcgac accggaatgc accgcttagg cattcacccg gagcaagtcg
    18661 atcactttgt tgaacgtttg aaaaattgtg ccttggtcaa ttcaatcagc tttgtgagtc
    18721 attttagccg tgccgatgaa ctcgattgtg gctataccga aaaacaaatt gcggtgtttg
    18781 agcaagctac gcaaaaatat caggatcacg accgcagtat ttccgcctca agcggtattc
    18841 tttattggaa acaggctcat tatgattggg ttcgcccggg tattattatg cacggcattt
    18901 ctccccacta tacgccgatt accgatttag gttttaagcc ggtaatgacg ctctcctctt
    18961 ccctgatcgc tgtgcgtacc cataaagccg gcgagcctgt aggctatggt ggtgcttggg
    19021 taagccctaa agataccaaa ctcggcgtga ctgcaatggg ctacggcgat ggctaccctc
    19081 gcaatgctcc tgaaggtacc cctgtgttga ttaacggtag aatagtgccg attgtcggtc
    19141 gagtttcgat ggatatgctg acggtcgatt taggtgcaga gagccaagat aaagtgggcg
    19201 ataaagccat tttttgggga gagcaactat taattgaaga tgtggcgaaa cacattggcg
    19261 tgattagcta tgagctgatt actaaactca ctcctcgtgt gatttttgaa tataaataga
    19321 caacaagcgg taaaattttc aacaaatttt accgcttgta tcttattcaa gatcgaaaat
    19381 attatttcgc acaaccgcag gttgctactt tttcttcaaa acggcgagcg gcttcgtccc
    19441 agtttacaac gttccagaat gctttaatga aatccggacg gcggttttgg tagtttaagt
    19501 agtaagcgtg ttcccaaacg tctaagacta aaatcggata gcctgaaacg cctgccactt
    19561 ctttacccat tagtgggttg tcctggttag cggttgaaac aacggctaat ttaccctctt
    19621 ctaatactaa ccacgcccag cctgaaccaa cacgagtagt cgctgctttt tcaaattctg
    19681 attggaaagc ttcaactgaa ccgaaatcac gctcaatcgc ttcttttaat gcaccttgta
    19741 aggtagtgcc tgtttttaaa cctttccaga ataaagtgtg gtttacatga ccacctacgt
    19801 tgttacgcac tgcaatacgt ttttctgcag gcacttggct caaatctttg attaatgcac
    19861 ccggacattt ttctaataat tcagggtgag cttctaatgc tgcatttgca ttgttgatgt
    19921 aagcttggtg atgtttagaa tggtggattt ccattgtttt agcatcgaaa tgtggctcta
    19981 acgcatcata agcgtagcct aattctggta atgtatatgc catagttaat tcctctcaaa
    20041 tagtaaatag gttaatcaga gttaaactcc gagtacattt tatcaagaaa cacacaaaaa
    20101 atgcacattt ttttgatcta gaacaaaatt atttcactat ctaatttaga aaggttccta
    20161 atcaaattct tgctt
//
LOCUS       DANLYI010000022        20133 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.022, whole genome shotgun sequence.
ACCESSION   DANLYI010000022 DANLYI010000000
VERSION     DANLYI010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 20133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20133
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.022"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            33..506
                     /gene="nrdG"
                     /locus_tag="PXD81_000612"
     CDS             33..506
                     /gene="nrdG"
                     /locus_tag="PXD81_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HDL5536667.1"
                     /translation="MENLRFSSEQIVWQEVPNETSLAFLITGCPLGCKGCHSADSWKA
                     CLGKELSSEYLSERLKRYQGLISCVLFMGGEWEAEKLLPLLQLAKKQGLNTCLYSGLE
                     QHEIAETLLHELTYLKTGRWIAERGGLESLTTNQRFTDLRNNQNLNHLFIRGASI"
     gene            503..2284
                     /gene="nrdD"
                     /locus_tag="PXD81_000613"
     CDS             503..2284
                     /gene="nrdD"
                     /locus_tag="PXD81_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005611280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HDL5536668.1"
                     /translation="MIRLQPTQLDGKLHFINQYIQAKNAADGSKMDANANVTQKNIAT
                     MEQELMKDFFVQINRAKVSEKIAKIFGKETACEYLRQIEAHEIYVHDETSLKPYCVSV
                     TMYPFLRDGLTKLGGESKAPKHLASFCGSFINFVFAVSSQFAGAVATVEFLTYFDYFA
                     RKDYGDNYLQTHRSDIENHLQQVVYSINQPAAARGYQSVFWNISVYDKFYFEAMFSRF
                     VFPDFEKPNWETTSKLQLFFMQWFNKERSKAILTFPVVTAAMLTEKGKCKDDEFANQM
                     AKELSQGNSFFIYQSDNPDSLASCCRLRNEISDHSFSYSLGAGGVATGSINVITINMN
                     RLVQDNRDLSEEVSKIHKYQYAYRKLMEEYLAAGMLPVYDAGFISLHKQFLTIGINGM
                     AEAAESQGLTVGYNPDYIDFVQSRLKIIFQANQAAGEKYGVKFNTEFVPAENLGVKNA
                     KWDKEDGYFVPRDCYNSYFYVVEDEEINALDKFLLHGRELIEWLDGGSALHLNLDEAL
                     TQTGYRSLLDTAAKTGCNYFCINVKITICNECSHIDKRTLHSCSKCGSDNIDYGTRVI
                     GYLKRVSAFSSARQKEHSLRFYHREAA"
     regulatory      2341..2437
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            2494..3312
                     /gene="thiM"
                     /locus_tag="PXD81_000614"
     CDS             2494..3312
                     /gene="thiM"
                     /locus_tag="PXD81_000614"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HDL5536669.1"
                     /translation="MTTSLPYHSHYLQKIRQNRPLVHNITNIVAANFSANGLLALGAS
                     PIMAAAIEEMESVPALSNAVVINIGTLIGKEVEAMELAGKTANRLGIPLILDPVGVGA
                     TPFRRQTVETLVDQIHFTAIRGNAGELATLAGVEWQAKGVDAGEGSHNVVEIAQQIAK
                     RYQTLVAISGETDVVSSGSHTALLHNGSPMFPKITASGCLLSAVCGAFLAVADKEHYF
                     NALIEACAAYAVAGEVAAQALKPTQYGQFAVNLLDTLGALTPETVEQYVRVEYV"
     gene            3305..4618
                     /locus_tag="PXD81_000615"
     CDS             3305..4618
                     /locus_tag="PXD81_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL5536670.1"
                     /translation="MSKTHSNPTKVAVASMVGTAIEYFDNYIYTMAAVLVFNVQFFNS
                     DDPVSNQLMSLSVLALAFFTRPMGSILFGHFGDKYGRKQTLIASLLLMGLSTVAIGLL
                     PNYAAIGIWATVLLCLCRIGQGLGLGGEWGGAALVATEHAPKHKRAFFGIFPQMGAPI
                     GLLLANGAFYLVNLIYGEQTFLDWAWRIPFVASIVMIAIGLYIRIKISESPIFLKAEQ
                     QGKNRHTPLKTLFSQHLKPFMIGVLIATAGYVLFYILVAFTPIFVKSPVAVSQAGHAT
                     GLGLPVNLYTQYLLITSVIFGIAIFFSGIYSDKIGRRLLLLLATGATVVYGLTMPFFL
                     ENGTPTSIFIFLTIGMILIGLSFGPMAVILPELFPTEVRYTGASLAYTAAGILGASVF
                     TIIAVKINENFGLFGVGGYLSVASLLSFWALWQVHETKDLELEEI"
     regulatory      4754..4850
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            4918..6447
                     /gene="thiE"
                     /locus_tag="PXD81_000616"
     CDS             4918..6447
                     /gene="thiE"
                     /locus_tag="PXD81_000616"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HDL5536671.1"
                     /translation="MSNYSNSPYQPNVIWTVAGSDSCAGAGLQTDLHTFHDFNLVGCS
                     VVTSVTAQHPHGVLCVTPVDDHTFRQQFEALLVQGYPNAIKIGLLCSQAQVEILCEYI
                     QKIRAESSHYCYVVYDPVAVASSGQALSDSILLQIVQQKLYPLVDLITPNGTELALLS
                     DTEIQTFGDVKTASHKLFAQGIKAVLAKGGHFEWLGETVRDYLLTANEIYAFSHARQQ
                     SVNTHGTGCTYASAVGAMLATGFDLADAVTVATAYLQKGLSEKQGRGQTALSSLCHTG
                     YPDDIAFFPQTELISAPLKLPKEPFAPTEYQLGLYPVVDSVKWLERLILVGVKTLQIR
                     IKNRPLAEIEQEIAHSVALAKQHNVRLFVNDYWQLAMKYQAYGVHLGQEDLATADLNA
                     IQQSGLRLGVSTHCYFEIMRALAVKPSYIAFGHVFPTQTKVMPSQPQGLTNLAKYVAL
                     VAPLNIPTVAIGGINEQSIENVMHTGVGSAAVVSAVTQAKDWQQAVKNLAKFANGGRN
                     E"
     gene            6440..7612
                     /gene="cytX"
                     /locus_tag="PXD81_000617"
     CDS             6440..7612
                     /gene="cytX"
                     /locus_tag="PXD81_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative hydroxymethylpyrimidine transporter
                     CytX"
                     /protein_id="HDL5536672.1"
                     /translation="MNRTLSTLNSGLIWAGAGISASAIVVGTWIAPLGWQQGLLAIVL
                     GHLLGGILFFAAGLIGAKTGKNAMQTVQISFGRGSVFFSLANVLQLVGWTAIMIYTGA
                     EISNALSLALWEYSAFSLWAIVLGLLIVLWLFTGSNQVGKFKLVTLIAMFLLTLWLSI
                     KVLNGQENFPVSGEMPFSTAVELATIMPLSWLPLVSDYTAKAKKPFAATLSATIGYLI
                     TSAWMYALGLGMVLFTGQTEIAPMLLMTGMSLIGIMVMILSTVTTTFLDAYSAGVSAG
                     NITAKFKETPTAILVTIIGTLLAISLPVTQYENFLLLIGSVFAPMIAVQIADFFVLKR
                     HQAQPNVDWVAAGLWLVGFVLYHLLSYHQVHLLLGLTIPVMLVIFLSTIFVRRFTK"
     gene            7651..7860
                     /locus_tag="PXD81_000618"
     CDS             7651..7860
                     /locus_tag="PXD81_000618"
                     /inference="COORDINATES: protein motif:HMM:NF015737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HDL5536673.1"
                     /translation="MESLVLNNDWVVTPQAQLAYHTINGKADEERLNLLVARAGVRVA
                     KGFALNNGWNLQPYAEVNGIAEKGQ"
     gene            7868..8044
                     /locus_tag="PXD81_000619"
     CDS             7868..8044
                     /locus_tag="PXD81_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HDL5536674.1"
                     /translation="MQVNQYQFGVAENKGRVQGSLGLSAGNGNHRLGLEASVASGKKL
                     KQPFNVLVNYRYQW"
     gene            complement(8085..9941)
                     /gene="sppA"
                     /locus_tag="PXD81_000620"
     CDS             complement(8085..9941)
                     /gene="sppA"
                     /locus_tag="PXD81_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HDL5536675.1"
                     /translation="MLTILKAFYRIFRCIRECVINLFFILFILLLVPVVAFISSSGST
                     QKPVFNQGALRLNLDGYLADNREEFTDFYRLVQSELGSSEPFKISTFDVVQAISHAKN
                     DPKITGLVLDLQKLQSADFSSLDFIGNEINNFKQSGKPVIAVGEDYSQKQYYLASFAD
                     EIYLNKVGAVDLKGLNYSNTYFKTLLDKIEAEPHIFRVGTYKSAVEPFMRDDMSEEAK
                     QNARGWLNGTWQQTVATLAQNRNIEPQALDLSPANYIEKYKQAKGDDAQLALNQKWVS
                     QLVSSQESRDKLIAQFGKNSEGSYNQIEFLDYMTELNDRFAEVNAPKIAVVNVEGAIA
                     GGESDEMNVGSETLVKLLQQARDDKNVEGLILRINSPGGSAVASELIRQEVEAFQQAG
                     KPVVASMGGMAASGGYWIAATSDKIIASPNTLTGSIGIFGLAVTFEKTAKNLGVSEDG
                     IATSALAQQAGLKSLPQEQAEVLQIGIENGYDRFLELVSRGRKMPKEVVDKVAQGQVW
                     LGTDALKHGLVDELGNFNVAYNALSELINQKREAENQPKVEQFGLQWFAEQDDSLFGT
                     LARDFKAQLQFSVANWLDLPLLKQPQQQLETLSRFNDPKQMYLYCLNCGKID"
     gene            10044..10607
                     /locus_tag="PXD81_000621"
     CDS             10044..10607
                     /locus_tag="PXD81_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HDL5536676.1"
                     /translation="MQILDLLHHRRSNKKFGDKAPNAEQLEQILKAGLRVPDHGRLKP
                     YRFVVIEKSGMPQFGECLKSAVAEFDMGEERLKKAEKLANQAPMIIGVVATLDDSIAK
                     VPSWEQMITAGCATYAIQLAANAQGFDTVWITNKWINGSALRSAFGCQESDKIIALIM
                     LGSPAEGEQIALAPESESLEGFVRYLK"
     gene            complement(10678..13002)
                     /locus_tag="PXD81_000622"
     CDS             complement(10678..13002)
                     /locus_tag="PXD81_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012263162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HDL5536677.1"
                     /translation="MTDLNLKISQIIAGELNVGSHQILAAITLLGEGNTIPFIARYRK
                     EVTGGLDDTQLRHFETRLIYLREMDERRQTILKSIEEQGKLTDELKAKILSSESKTEL
                     EDLYLPYKPKRRTRGQIAIEAGLEPLADSLWNDPKQDPETLAANFVDAEKGVADTKAA
                     LDGARYILMERFSEDAELLAKLRQYLTAYATLESKVIDGKEEEGEKFRDYFAHSEPFN
                     SVPSHRALAMFRGRNEGVLSLSLNADPTAEEGTKSSHCEEIIREHLGVIFNNQPADKW
                     REQVIAWTWKIKAALHLETELMATLCEKAEEEAIDVFARNLSALLMAAPAGARNTMGL
                     DPGLRTGVKVAVVDNTGKLLDTATIYPHTGREAEAQLAIFSLIKQHNVELIAIGNGTA
                     SRETERFAKAVIKEIKENKPQTVVVSEAGASVYSASEFAANEFPNLDVSLRGAVSIAR
                     RLQDPLAELVKIEPKAIGVGQYQHDVNQSQLARKLDAVVEDCVNAVGVDLNTASAPLL
                     ARVAGMTKTLAQNIVAFRDENGRFTSRSELKKVPRLGPKAFEQCAGFMRIIGGKNALD
                     ASSVHPEAYPVVEKILQATTSTLAELMGNNSKIHSLNAKDFVDEQFGLPTVNDIFKEL
                     EKPGRDPRGEFKTAVFMDGVEEITDLKAGMILEGTVTNVANFGAFVDIGVHQDGLVHI
                     SMLSNSFVEDPHTVVKAGDVVKVKVLEVDAARKRIALTMRLDDKPTDKKEPTSGRNSA
                     EICKNTQKNDRLQSNNRNAFGNNAFADALKGWKK"
     gene            complement(13002..13556)
                     /gene="pilW"
                     /locus_tag="PXD81_000623"
     CDS             complement(13002..13556)
                     /gene="pilW"
                     /locus_tag="PXD81_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis/stability protein PilW"
                     /protein_id="HDL5536678.1"
                     /translation="MFAKFFPKMTACILIFLLAACSSLPPSSSEVEFNRSEAVKARIN
                     LALAYLEQNDFPKAKQNIDKALAHDEKDYLPYSVLAYYYQQTGDAENAEKAYQTALKL
                     SQNRPDVLNNYGTFLCKQGQFQQAYQQFEQAVKSKAPYYHQADSLENMVLCAKKEPNL
                     QKIAETLTELEKLDKPRANLLKEN"
     gene            13776..14027
                     /locus_tag="PXD81_000624"
     CDS             13776..14027
                     /locus_tag="PXD81_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDL5536679.1"
                     /translation="MKIVVFQRKALKQLRKIPTGAEIRRKCDDLAYFPYCANIKALTN
                     HQYDYRFRVGNYRIFFNIEGETMNIVSIEEVKKRDERTY"
     gene            14011..14358
                     /locus_tag="PXD81_000625"
     CDS             14011..14358
                     /locus_tag="PXD81_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015702293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL5536680.1"
                     /translation="MKELTNIQYLNNEEGKPAFAVLPIATLEWLKQKANIKTAIETGI
                     PSAVAGIALDNNYSALRAWREYLGLTQEEVAQRLGISQSAYSQHENAKNLRKTTRIKI
                     AYALGLNEEQLDF"
     gene            complement(14399..15559)
                     /gene="trmG/rlmN"
                     /locus_tag="PXD81_000626"
     CDS             complement(14399..15559)
                     /gene="trmG/rlmN"
                     /locus_tag="PXD81_000626"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HDL5536681.1"
                     /translation="MSELITNAGCGSAEQPKEKINLLNLNRQEMRELFAEMGEKPFRA
                     DQLMKWIYHFGEENFDNMSNINKVLREKLKRIAEIKAPEVAVEQRSADGTIKWAMQVG
                     DQQIETVYIPEDDRATLCVSSQVGCALACTFCSTAQQGFNRNLSVAEIIGQVWRASKI
                     IGNFGVTGVRPITNVVMMGMGEPLLNMNNVIPAMEIMLDDFAYGLSKRRVTLSTSGVV
                     PALDKMREQIDVALAISLHAPNDELRDELVPINKKYNIKMLMDSVNKYREVSNANHGK
                     VTIEYVMLDHVNDSTNHAHQLAEVLKNTPCKINLIPWNPFPEAPYGKSSNSRVDRFQK
                     TLMEYGFTVTVRKTRGDDIDAACGQLAGDVIDRTKRTLEKRKFGQGIAVKTH"
     gene            15819..16715
                     /locus_tag="PXD81_000627"
     CDS             15819..16715
                     /locus_tag="PXD81_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HDL5536682.1"
                     /translation="MKLSTIKKALVLTFGLSAAMAMAEPFKVVTTFTVIQDIAQNVAG
                     DKATVESITKPGAEIHDYQPTPKDIVKAQKADLVLWNGMNLERWFERFFENVKGKPAV
                     VVTEGITPMPITEGEYKNLPNPHAWMSTDNALIYIENIRAALVKYDPQNADTYNANAK
                     AYAEKVKGIAEPLRQRLAFIPEQQRWLVTSEGAFSYLAKDYNLKELYLWAINAEEQGS
                     PKQVKKVIDGVKANKIPVVFSESTVSDKPAKQVAKETGALYGGVIYVDSLSEKDGPVP
                     TYLDLLKVTIGTIVEGFERSKK"
     gene            16728..17471
                     /locus_tag="PXD81_000628"
     CDS             16728..17471
                     /locus_tag="PXD81_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HDL5536683.1"
                     /translation="MTTVSASISVENLSVRYNNGHIALQDVSFHLENGTICALIGVNG
                     GGKSTLFKSLMGLVKPLTGSVKLSQLPIAKALKQNLVSYVPQSEEVDWQFPVSVYDVV
                     MMGRYGYMNFLRRPSQTDKLKVEQAMQRLDVLHLKDRQIGELSGGQKKRVFLARALAQ
                     ESKIILLDEPFTGVDVKTENAIVELLKQLKNEGHLILVSTHNLASVPSFCDQVLMVNR
                     TLLAKGKTEEIFNNQNLERVFGGLLHYQK"
     gene            complement(17528..18337)
                     /locus_tag="PXD81_000629"
     CDS             complement(17528..18337)
                     /locus_tag="PXD81_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HDL5536684.1"
                     /translation="MLNWILEPLQFEFMQIALITAILVAGVSAVLSCYLVLKGWSLMG
                     DAISHAVLPGVVISSLAGLPLSIGAFFSGLGCALSVGYLKENSRLKEDTVMGIVFSGM
                     FAFGILLFTAFNTGEHLTHILFGNLLGVTKSDLLQTLLISLFTFVVMVAKRKDFLLYC
                     FDVNQAKVAGLPVRLLHYGLLSLLALTIISAMQVVGVILVVAMLITPGITAYLLTKRF
                     DSMLLIALGVAIFSSVFGTLLSYHIDAATGPTIILVQAAIFFLSFAYSKIH"
     gene            complement(18330..19190)
                     /locus_tag="PXD81_000630"
     CDS             complement(18330..19190)
                     /locus_tag="PXD81_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HDL5536685.1"
                     /translation="MLDLLLEPFTYNYMQKAIFVSMGVGLVCAFLSAFLMLKGWSLIG
                     DALSHAVVPGVAIAYALKLPYAFGAFFSGILAALSILWVKSLTKIKEDAVIGFIFTTF
                     FALGMFIVSLNPTSVDVNSIVMGNILGIADDDLWQVVLIIAISLLGLILFWKDLLLVF
                     FDEHHALSVGLSPLRYKVIFFTLLSACVVVALQTVGAILVIAMVVTPGATAYLLTDRF
                     PRLVLIAMLIGTLTSGIGAYLSYFLDGATGAVIVCLQTLIFLFAFCFAPKYGLITQKR
                     KLHRVEVQNA"
     gene            complement(19264..20049)
                     /locus_tag="PXD81_000631"
     CDS             complement(19264..20049)
                     /locus_tag="PXD81_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HDL5536686.1"
                     /translation="MMKKITILGLGWLGLPLAEALQNKGWRVAGTKRTVGELAIECYP
                     LDLNALAVTSEIEQLLAADALVIALPLSQIRPENYGCGIQTLVSLAIEKGLKHLVFTS
                     STSVLPMKSGVFDENAVVDSANLLVKMENWLLSQPIHCDILRLAGLVGKKRHPVFYLA
                     GKQNLSGAGQPVNLVHLEDCIAAISLLLEQPNGQRIFHLCAEQHPSRKDYYSEMAKRF
                     GLADLHFSEENQPLVRLIKADKICQELGFRYYYPDPYQFKVDI"
BASE COUNT         5780 a         4505 c         4231 g         5617 t
ORIGIN      
        1 aaaacacaat atattgtgtt aatgaattag taatggaaaa tctcagattc agctcggaac
       61 aaattgtttg gcaagaagtg ccgaatgaga cctcacttgc ttttctgatt acaggctgcc
      121 cgcttggctg taaaggttgc catagtgcgg atagctggaa agcctgtctt gggaaagagc
      181 ttagcagtga atatctctct gaacgcctaa aacgctacca aggcttaatt agctgcgtat
      241 tgtttatggg cggagaatgg gaagcagaaa aattactgcc gctgttgcaa ctggcaaaaa
      301 aacaggggct aaacacctgt ctttatagtg gtttggagca gcacgaaatc gccgagactt
      361 tattacacga gctaacctac ttaaaaaccg ggcgttggat tgccgaacga ggaggattgg
      421 aaagtttaac caccaaccaa cgctttactg atttacgcaa taaccaaaat cttaaccatc
      481 tttttattcg aggagcttca atatgattcg cttacaaccg acacaattag acggaaaact
      541 acatttcatt aaccaatata ttcaagccaa aaatgccgca gacggttcaa aaatggacgc
      601 caacgctaac gtcacgcaaa aaaacatagc gacaatggaa caggagctaa tgaaagattt
      661 ttttgtgcaa atcaaccgag caaaagtgag tgaaaaaatt gcaaaaattt ttggaaaaga
      721 aaccgcttgt gaatatttac gtcaaatcga agcacacgaa atttacgtac acgatgaaac
      781 cagcctaaaa ccctactgcg tttcagtcac tatgtaccct ttcttgcgtg atggtttgac
      841 taaactcggc ggcgaatcca aagccccgaa acatttagcc tcattctgcg gttcttttat
      901 caattttgtc tttgcagtca gttctcagtt tgccggtgca gtcgccacgg ttgaatttct
      961 gacctatttt gactattttg cccgcaaaga ttacggcgat aattacttac agactcatcg
     1021 ttcagatatt gaaaaccacc tgcagcaggt tgtgtatagc atcaaccaac cggcagccgc
     1081 tcgtggttat caaagcgtct tttggaatat ttcagtgtat gacaaattct attttgaagc
     1141 gatgtttagc cgttttgttt ttcctgattt tgagaaacca aattgggaaa ccacttcaaa
     1201 attacaactt ttctttatgc aatggttcaa taaagagcga agcaaagcga ttctcacttt
     1261 ccctgttgtg accgcagcaa tgcttaccga aaagggcaaa tgtaaagatg acgagtttgc
     1321 caatcaaatg gcaaaagagc tatctcaagg caattcattc tttatttatc aatcagataa
     1381 tccggattcc cttgcttctt gctgccgctt gagaaatgaa atcagcgatc atagcttctc
     1441 ctattcatta ggggctggag gtgttgcgac aggttcaatc aatgtaatta ctattaatat
     1501 gaaccgtttg gtgcaagata accgtgattt aagcgaagaa gtcagcaaga tccataaata
     1561 tcaatacgct taccgcaaac tgatggaaga gtatttagct gccggtatgt tgccggttta
     1621 cgatgccggt tttatttcac tgcataaaca attcctgact attggcatta acggaatggc
     1681 agaagctgct gaatcacaag gtttaaccgt aggttacaat ccagactata ttgattttgt
     1741 gcaatctcgg ttaaaaatta ttttccaagc taaccaagca gccggtgaaa agtatggggt
     1801 aaaatttaat accgaatttg tgcctgctga aaatttaggg gtcaaaaacg ccaaatggga
     1861 caaagaggac ggctatttcg taccacgaga ttgctacaat tcctatttct atgtggtaga
     1921 agatgaagaa attaatgctt tggataaatt cttactgcac ggcagagagc tgattgaatg
     1981 gctggacgga ggctccgctc tacacttgaa tttagacgaa gccttaaccc aaaccggata
     2041 tcgctccttg ctggataccg ccgctaaaac cggctgcaat tatttttgta ttaacgttaa
     2101 aatcacgatt tgcaacgaat gtagccacat cgacaaacgc acgttgcaca gttgtagcaa
     2161 atgtggctcc gataatattg attacggcac tcgggtaatc ggctatttaa aacgagtctc
     2221 cgcctttagt tccgcccgcc aaaaagagca cagcttgcgt ttctatcacc gagaagcagc
     2281 ttaattgaaa actgcagttg tagcccttta caaccgcttt tttagtgatt atattaaagc
     2341 ctcatttagt cggggtgctt gcctaagggc aagctgagaa atacccgttg aacctgatgc
     2401 agttagcact gacgtaggaa actaaaggcg taaacccatc tattccccgt tggtgttggt
     2461 gcgttgtttt tcaatcatat taaggaataa acgatgacca catcactccc ctatcactcg
     2521 cattatttgc aaaaaattcg acaaaatcga ccgcttgttc acaacattac caacattgtt
     2581 gccgctaatt tttctgccaa cggcttattg gctcttggtg cttctcccat tatggcagct
     2641 gctattgagg agatggaatc cgtgccggca ctcagtaacg ccgtggtgat taatatcggc
     2701 acgcttatcg gcaaagaagt agaagcaatg gagcttgctg gcaaaaccgc caaccgttta
     2761 ggtattccgc tcattcttga ccctgtcggc gttggagcaa cacctttccg ccgtcaaaca
     2821 gttgaaacct tagtcgacca aattcacttc accgccattc gtggaaatgc gggggaacta
     2881 gctaccctcg ccggtgtaga atggcaagcc aaaggcgttg atgccggcga aggctcgcat
     2941 aatgtggttg aaatcgccca acagattgcc aaacgttacc aaaccttagt cgcaatcagt
     3001 ggtgaaaccg atgtggtaag ctctggctca cacacagctt tattgcacaa cggcagcccg
     3061 atgttcccca aaattaccgc ttcaggctgt ttattaagtg cggtatgcgg ggcattctta
     3121 gccgttgccg acaaagagca ctatttcaac gcacttattg aggcttgcgc cgcttatgcc
     3181 gttgcaggcg aagtggctgc tcaagcactc aaaccaactc aatacgggca atttgccgtc
     3241 aatctgctgg atacccttgg ggcattaacg cctgaaaccg ttgagcaata tgtgagggtt
     3301 gaatatgtct aaaactcact caaacccaac taaagtggcg gttgcctcaa tggtcggcac
     3361 ggcgattgaa tattttgata actacatcta cacgatggca gcagtgctgg tgtttaatgt
     3421 gcagtttttt aatagcgatg atccggtttc caaccaatta atgtcgcttt ccgtactagc
     3481 tcttgccttt ttcacccgcc cgatggggtc gattttattc gggcatttcg gcgataaata
     3541 cggacgcaaa caaactctga ttgcctcgct cttattaatg ggcttatcta ccgtagcaat
     3601 cgggcttttg cctaattatg ccgccattgg catttgggca accgtgttac tctgcctctg
     3661 tagaatcgga caaggtttag gcttaggcgg cgaatggggc ggagctgctc tcgttgccac
     3721 tgaacacgcc ccgaaacaca agcgggcatt ttttggtatt tttccgcaaa tgggcgcccc
     3781 aatcggcttg ctgttggcaa acggagcgtt ctacttggtg aatttaattt acggtgaaca
     3841 aaccttcttg gattgggcgt ggcgaattcc gtttgtcgct tctattgtga tgatcgccat
     3901 tgggctttat atccgaatta agatcagcga aagcccgatt ttccttaaag cggaacaaca
     3961 aggtaaaaac cgccataccc cgcttaaaac tttgtttagc caacacctca agccttttat
     4021 gatcggcgtg ctgattgcca ccgccggcta tgtgttgttc tacattttag ttgctttcac
     4081 cccgattttc gtcaaaagcc cggtggcagt ttctcaagca ggacacgcta caggcttagg
     4141 tttaccggta aatctctaca cccaatattt gctgattacc tccgtgattt tcggcattgc
     4201 gattttcttc tccggcattt attcagacaa aatcggacgc cgtttactgc tgttacttgc
     4261 cacaggagca accgtagttt acggcttaac aatgccgttt ttcctcgaaa acggcacacc
     4321 gacttcgatc tttattttcc tcaccattgg aatgatctta atcggcttaa gcttcggccc
     4381 gatggcagta attctacccg aactcttccc aacagaagta cgttacacag gggcttcgct
     4441 tgcctacacc gctgccggta ttttaggagc tagcgtgttc actattattg cggtcaaaat
     4501 caatgaaaat ttcggcttat tcggtgtagg cggctattta tcggttgcct cattactgag
     4561 cttctgggca ttgtggcaag tccacgaaac caaagatttg gaattagagg agatataaaa
     4621 aaccactcac ttaacgacgg ggcaagttac caccttgccc caagatgagg gaatgcaaat
     4681 ttccctctaa tagacttcac aacggatatt ttgcattttt ttataaaaaa taaccgcttg
     4741 taagctatac taaaaacgct tgtcggagtg ccgtaaggct gagatcgcaa cagcgagatc
     4801 cgtggaacct gtaggttagt acctgcgtag ggaacaagtt ttacccaaat tcagattttg
     4861 ggatcattac cacatctctg gcaagcaaaa ccctaagcat attatgagga ttttgcgatg
     4921 tctaattatt caaactctcc ataccaacct aacgtcattt ggacggttgc cggctcagat
     4981 tcctgtgccg gtgccggtct gcaaaccgat ttacatacct ttcacgattt taacctagtg
     5041 ggttgttcag tagttacttc tgtaacggct caacatccgc acggtgtgct gtgtgtaacg
     5101 cctgttgatg atcacacctt ccgccaacag tttgaagcct tgctggttca aggctacccg
     5161 aacgcaatca aaatcggctt actctgctcg caagcacaag tagagatctt gtgtgaatat
     5221 atccaaaaaa tccgtgcaga aagctcgcat tattgttatg tggtttacga ccctgtggca
     5281 gtggcaagca gtgggcaggc gttatccgac agtattttat tgcagatagt gcaacaaaaa
     5341 ttgtatccgc tggtggattt aatcactcca aacggcactg aactcgcttt attaagcgac
     5401 acagaaatcc agacctttgg tgatgtgaaa accgctagcc acaaactgtt tgcacaggga
     5461 atcaaagccg tgttagccaa aggtggacat tttgaatggc tgggggaaac ggtaagagat
     5521 tatctgctga ctgcaaatga aatttatgcc tttagtcacg cccgacagca aagtgtgaat
     5581 acccatggca caggttgcac ctatgcttca gcggttggag cgatgttggc aaccggcttt
     5641 gatttagctg atgccgtcac tgttgccacc gcttatttgc aaaaaggctt atcggaaaaa
     5701 caaggcagag ggcaaactgc tttaagttca ctttgccaca ccggctaccc tgatgatatc
     5761 gctttcttcc cacaaaccga gctgatttct gcccctctta aattgccgaa agagcctttt
     5821 gctccaacgg aatatcaact tgggctttac cctgttgtag atagcgttaa atggcttgaa
     5881 cgcttaattc ttgtaggggt aaaaaccttg caaattagaa tcaaaaatcg accgcttgca
     5941 gagattgagc aagaaattgc ccattcggtc gcactggcga aacagcacaa tgtgcgtttg
     6001 tttgtaaatg attactggca gttagccatg aagtatcagg cttacggcgt gcatttaggg
     6061 caggaggatt tagccaccgc cgatttaaat gcaattcagc aatctgggtt acggcttggt
     6121 gtttcaaccc actgctactt tgaaataatg cgggcattgg cggtgaaacc gtcttatatc
     6181 gcttttggcc acgttttccc tactcaaacc aaagtaatgc cgtctcaacc acaaggctta
     6241 acaaatttag ccaaatatgt tgctctcgtt gcaccgctta acattccaac tgtggcgatt
     6301 ggtggaatta atgagcaaag cattgaaaat gtaatgcata ccggtgtcgg cagtgccgcg
     6361 gtggtaagtg ccgttaccca agcaaaagat tggcaacaag cggtcaaaaa cttagcaaaa
     6421 tttgcaaatg gaggccgtaa tgaatagaac tcttagtacg ctaaacagtg gtttgatttg
     6481 ggcgggagcg ggcattagtg ccagtgcaat cgtggttggc acttggattg ccccacttgg
     6541 ctggcaacaa ggcttgctgg cgatagtgct agggcattta ttaggcggca ttttgttttt
     6601 tgctgccggc ttaattgggg cgaaaaccgg caaaaatgcg atgcaaacgg tgcaaatttc
     6661 ctttggcaga ggctcggtgt ttttctccct tgccaatgtg ctacagcttg ttggttggac
     6721 agccattatg atctatacag gggcagagat cagtaatgcc cttagccttg ccttatggga
     6781 atattcggct ttctcgcttt gggcaatcgt gcttgggttg ctgattgtgc tatggttatt
     6841 taccggctct aatcaggtcg gaaaattcaa gttagtgaca ttgatagcga tgtttttgct
     6901 caccttatgg ctaagcatta aggtattgaa tgggcaggaa aattttcctg tttcgggtga
     6961 aatgccgttt agcaccgcag tggaattagc aactattatg cctctttcgt ggctaccttt
     7021 ggtatcagac tataccgcta aagcgaaaaa gccttttgcc gcgacccttt cggcgaccat
     7081 aggctatttg atcaccagtg cgtggatgta tgcgttaggt ctgggtatgg tgctatttac
     7141 cgggcaaacc gaaattgccc ctatgttact aatgacagga atgagcctta tcggcattat
     7201 ggtgatgatt ctttcgactg tgactaccac gtttcttgat gcctattccg ccggtgtcag
     7261 tgccggcaat atcacggcaa aattcaaaga aaccccaacc gccattctag tcacaatcat
     7321 cggcacgcta ctggcaatca gtttaccggt tacccaatat gaaaatttcc tgctgctgat
     7381 aggctcggta ttcgccccga tgattgccgt gcaaattgcg gatttctttg tgttaaaacg
     7441 ccaccaagca cagcctaatg ttgattgggt tgctgccgga ttatggcttg tcggcttcgt
     7501 gctataccac cttctctctt accaccaagt gcatttacta ctcggcttaa ccattccagt
     7561 gatgttagtg atttttctca gcaccatttt tgtgaggagg ttcacaaaat agaaagataa
     7621 gcggttcaat tggagtgaaa atttgctaaa attgagtcgc ttgttttaaa caatgactgg
     7681 gtagtaacac cacaagctca acttgcctac cacaccatta acggtaaagc cgatgaggaa
     7741 cggttaaatc ttttagttgc ccgtgccggc gtaagagtag caaaaggctt tgccttgaat
     7801 aatggttgga atctgcaacc ttatgcggaa gtaaacggca ttgctgaaaa aggccaatag
     7861 tacgaaagtg caagtaaacc aatatcagtt tggagttgcc gaaaacaagg gcagagtgca
     7921 aggttcactt ggcttgagtg caggaaacgg taatcaccgt ttaggtttag aggcatcagt
     7981 tgcttcaggt aagaaactga aacaaccatt taatgtatta gtaaattatc gttatcaatg
     8041 gtaatttaag agggcaggtg aaaacctgcc cttaaattca ataattaatc tatcttccca
     8101 caatttaaac agtaaagata catctgtttc ggatcgttaa aacgtgaaag agtctctagc
     8161 tgttgttgcg gttgcttcaa cagcggtaaa tcaagccaat ttgccacgct aaattgtaat
     8221 tgagctttga aatcacgggc aagcgtgcca aataagctgt catcttgctc cgcaaaccat
     8281 tgtaagccga actgttcgac tttaggttgg ttttccgctt cacgtttttg gttgatcaat
     8341 tcagataacg cattatatgc cacattaaaa ttgcccaact catcaaccaa tccgtgtttt
     8401 aacgcatcag ttcccagcca cacttgccct tgagccactt tatctaccac ctcttttggc
     8461 attttcctgc cacgagaaac cagctctaaa aaacggtcgt agccgttttc aatcccaatt
     8521 tgcagcactt ctgcctgctc ttgcggtagg ctttttaaac cggcttgttg tgctagggca
     8581 gaagtcgcta tgccgtcttc gctcacaccg agatttttag cggttttttc aaaagttact
     8641 gctaaaccga aaataccgat agagccggta agcgtattcg ggctagcaat aattttatcg
     8701 ctggtagccg caatccaata accgcccgat gctgccattc cgcccattga agcaaccacc
     8761 ggtttgccgg cttgttgaaa ggcttccact tcttggcgaa tcagttcaga cgccacagca
     8821 ctgccgccgg ggctgttaat gcgtagaatt aagccttcca catttttgtc atctcgagct
     8881 tgttgcagta attttaccag cgtctcgctg cccacattca tttcgtcact ttcaccgcca
     8941 gcaatcgccc cttccacatt cactaccgca attttcggag cattcacttc tgcaaagcgg
     9001 tcgtttagtt cagtcatata atctaaaaat tcaatctggt tataactgcc ttcgctgttt
     9061 ttgccaaact gtgcaatcag tttatcccga ctttcttggc tggacactaa ctgactaacc
     9121 catttttgat ttaatgccag ttgagcatca tctcctttag cttgtttata tttttcaatg
     9181 taattggcag gcgataaatc cagtgcttgc ggttcaatgt tgcggttttg agctagtgtt
     9241 gccacagttt gttgccaagt gccgtttaac cagcctcggg cattttgttt cgcctcctca
     9301 gacatatcat cacgcataaa cggctcaacc gccgatttat aagtaccgac acggaaaata
     9361 tgcggttcag cctcaatttt atcgagcaag gttttgaaat aagtgttgga ataatttaag
     9421 ccttttaaat ccaccgcacc gactttattg agataaattt catcagcaaa ggaagcaaga
     9481 taatattgtt tttgcgagta gtcttcgccc actgcaatca ccggcttgcc cgattgtttg
     9541 aagttgttga tttcattgcc gataaaatcc aatgagctga aatcagcact ttgtagcttt
     9601 tgcagatcta acaccaagcc ggtaattttc gggtcatttt tagcgtggct aatcgcttgc
     9661 accacatcaa aagtcgagat tttaaacggc tcggaactac ctaattccga ttgcaccaag
     9721 cggtagaaat cagtgaattc ttcccgatta tctgctaaat aaccatctaa atttaaacgt
     9781 aacgcccctt gattgaacac cggcttttgc gtgctgccag aagagctgat aaatgcgacc
     9841 accggcacta atagcaaaat aaacagaata aaaaagagat taatgacaca ctctctaata
     9901 caacggaaga ttcggtaaaa tgcttttaaa atcgttaaca tcggaaattc ctaaaaataa
     9961 actcagtcgg cacagtataa agcaatcgct aaatgaagaa aatatgctat tcttgaacgc
    10021 aaaaaattaa caaaggaacg cttatgcaaa tcttggattt attacaccat cgccgttcaa
    10081 ataaaaaatt tggcgataaa gccccgaatg cagaacaact ggaacaaatt ctaaaagccg
    10141 gcttgcgagt gccggatcac gggcggttaa agccttatcg ctttgtggtg attgaaaaaa
    10201 gcggtatgcc acagtttggt gaatgcttaa agtctgcggt agcagaattt gatatgggcg
    10261 aggaacgctt aaaaaaagcg gaaaaattag ctaatcaagc tccaatgatt atcggcgtgg
    10321 tagcaacatt agacgacagc atcgccaaag tgccaagttg ggaacaaatg attaccgcag
    10381 gctgtgcaac ttatgcgatt cagttagcgg caaatgcaca gggctttgat acggtttgga
    10441 tcaccaataa atggattaac ggctctgctc ttcgctccgc ttttggttgc caagaatctg
    10501 ataaaatcat tgccttaatt atgcttggct caccggcaga gggcgagcaa attgcattag
    10561 cccctgaaag tgaaagttta gaggggtttg tacgttatct taagtaagca ggttaacaga
    10621 taaaagttag taataagggc gaaaaatatt tcgcccttgc ttttatctgt tttcgcctta
    10681 cttcttccag ccttttaacg catcggcaaa ggcattgtta ccaaaagcgt tgcgattgtt
    10741 gctttgcaag cggtcatttt tctgcgtatt tttgcaaatt tctgctgaat ttcgaccgct
    10801 tgttggctct tttttatcgg ttggtttgtc gtctaaacgc atcgtaaggg caatacgttt
    10861 gcgggcagcg tccacctcaa gcactttgac tttcaccaca tcaccagctt tcactaccgt
    10921 gtgcggatct tcaacaaaag agtttgagag cattgaaata tgcaccaagc catcttggtg
    10981 tacgccaata tcgacaaaag ccccaaagtt tgccacattg gtcactgtgc cctccaaaat
    11041 cataccggct ttgaggtcgg taatttcttc cacgccgtcc ataaacacgg cggttttaaa
    11101 ctctccacga gggtcacgcc ccggtttttc taactctttg aaaatatcgt tcaccgttgg
    11161 taaaccaaac tgctcatcca caaaatcttt ggcgttgagc gagtgaattt tgctgttgtt
    11221 acccattaat tctgcaagtg ttgaggtagt tgcctgtaag attttttcta ccaccgggta
    11281 agcttccggg tgaacgcttg aggcatcaag ggcatttttc ccaccgataa ttcgcataaa
    11341 accggcacac tgttcaaagg ctttcggccc taaacgaggc acttttttca gctctgagcg
    11401 gctggtaaaa cggccgtttt cgtcacggaa tgccacgata ttttgtgcca atgttttggt
    11461 cattcccgcc acccgagcca gcaacggagc ggaggctgta ttcagatcca cgcccactgc
    11521 gtttacacaa tcttctacta ccgcatcgag tttacgggca agttgggatt ggtttacatc
    11581 gtgttgatac tgcccaacac caatcgcttt tggttcgatt ttcaccaact cggcaagtgg
    11641 atcttgcaaa cggcgggcaa tcgaaaccgc tccccgtaat gaaacgtcta aatttggaaa
    11701 ctcgttagcc gcaaattcag aggcagaata gaccgatgcc ccagcctcac tcaccaccac
    11761 cgtttgcggt ttattctctt tgatttcttt aatcaccgct ttggcaaaac gttcggtttc
    11821 acgagaggcc gtaccgttac caatcgcaat cagttccaca ttgtgttgtt tgattaagct
    11881 gaaaatcgca agctgtgcct ccgcctctcg cccagtgtga gggtaaattg tggcggtatc
    11941 cagcaattta ccggtgttat ccaccaccgc cactttcacg ccggtacgca agcccgggtc
    12001 tagccccatt gtatttctcg caccggcagg ggctgccatt aataatgccg agagattgcg
    12061 ggcaaacaca tcaatcgcct cttcttcagc tttttcacac agggttgcca ttaattcggt
    12121 ttctaaatgc agagctgctt tgattttcca cgtccaagca atcacttgct cacgccattt
    12181 atctgctggt tgattattaa aaatcacgcc caaatgctca cgaataattt cttcacaatg
    12241 gctggatttt gtgccttctt ctgcagtcgg atcggcattt aatgaaagtg ataacacccc
    12301 ttcatttcgc ccacggaaca tcgccaaagc acggtgggaa ggcacgctgt taaacggctc
    12361 gctatgagca aaataatcac ggaatttctc gccctcttcc tctttgccgt caatcacttt
    12421 cgattccaac gtggcgtaag cggtcaaata ttggcgaagt tttgcaagta gctcagcatc
    12481 ttcgctaaaa cgctccatta aaatatatct tgccccgtct aatgcggcct tggtatccgc
    12541 aacgcctttt tccgcatcaa caaagtttgc cgctaaggtt tcaggatctt gtttcggatc
    12601 attccataaa ctgtccgcca atggctctaa ccctgcttca atcgcaattt gccctctggt
    12661 gcgacgtttt ggtttatacg gcaaatagag atcttccagc tcggtcttgc tttcactgct
    12721 tagaattttg gcttttaatt catcggttaa tttgccttgt tcttcaatcg acttcaaaat
    12781 ggtttggcgg cgttcgtcca tttcacgcaa ataaattaag cgggtttcaa aatggcgaag
    12841 ttgggtgtcg tctagcccgc cggtcacttc tttacggtaa cgagcaataa atggaatggt
    12901 attgccctca cctaatagcg tgatggctgc aagaatttgg tgtgagccaa cgttgagttc
    12961 gccggcaata atttggctga tttttagatt taagtcagtc attagttttc cttcaataaa
    13021 tttgctcgag gtttatccaa tttctcaagc tcggtcaatg tttcggcaat tttttgcaga
    13081 ttcggctctt ttttcgcaca taacaccata ttttctaagc tatccgcttg gtgatagtag
    13141 ggtgctttgc ttttgacagc ctgctcaaat tgctgataag cctgttgaaa ttgaccctgt
    13201 ttacataaaa aagtgccgta attattcagc acatcagggc ggttttgact caatttcagt
    13261 gccgtttggt aggctttttc ggcattttca gcatcacctg tttgttggta ataataggca
    13321 agcaccgaat aagggaggta atctttttca tcgtgagcca aggctttatc aatattctgc
    13381 ttagcttttg ggaaatcatt ttgctccaga taagctaaag ccaaattaat acgggctttt
    13441 accgcttccg agcggttaaa ttcgacttca gaggaagaag ggggaagaga agaacaagcc
    13501 gctaataaaa aaatcagaat acaagcggtc atttttggaa aaaattttgc aaacataacg
    13561 ttctcctaca acaaagggct taattttact gttccatcct cgctaagact agcgattttt
    13621 tgcgaagaag aaaaattttt cgttttcatt tttcgacgca gatcgcaaaa ataataatgg
    13681 ggtgttgtgc tagggtaaat ggtaggaata cactctctat ttaatataag aatttcattg
    13741 aaaaaactta taaaacttat aaaatagagg caaaaatgaa aatagtggta ttccagcgta
    13801 aagccttaaa gcaattacga aaaatcccaa ctggtgcaga aattcgccga aaatgtgatg
    13861 atttagctta ctttccctac tgtgccaata ttaaggcatt aacaaatcat caatatgatt
    13921 atcgctttcg ggtcggaaat taccgtattt ttttcaatat tgaaggcgaa acgatgaata
    13981 ttgtgtctat cgaagaggtt aaaaaacgag atgaaagaac ttactaatat tcaatattta
    14041 aataatgaag aaggaaaacc tgcctttgcc gtacttccga tagcaacact tgaatggctt
    14101 aagcagaaag caaatattaa aaccgcaata gagaccggta ttccatctgc tgttgctgga
    14161 attgcattag ataataacta ctcagctctg cgtgcttggc gggaatattt aggtttaact
    14221 caagaagagg tggcacaacg cttggggatt tcgcaatcag cttatagcca acacgaaaat
    14281 gcaaaaaatc tacgaaaaac gacccgcata aaaattgcct atgcgttggg tttaaatgaa
    14341 gagcaattag atttctaata acaaaaaggc aggaacctct gccttttcac tattcagatt
    14401 aatgggtttt aaccgcaata ccttgcccga atttacgttt ttcaagggtg cgtttggtgc
    14461 ggtcaatcac atcgccagca agctgtccgc aagcggcatc aatatcatca cctcgtgttt
    14521 tacgcactgt taccgtgaaa ccgtactcca tcaaggtttt ttggaaacga tctacccgag
    14581 agttagagct tttaccataa ggagcttctg ggaacgggtt ccacggaatc aagttaattt
    14641 tacacggcgt gttttttaat acttcagcaa gttggtgggc gtggtttgtg ctgtcattga
    14701 catgatctaa catcacatat tcaatcgtga ccttgccgtg gttggcgtta gaaacttcac
    14761 ggtatttatt caccgaatcc atcaacattt tgatgttgta ctttttatta atcggcacta
    14821 attcatcacg cagttcatcg ttcggagcgt gaagtgaaat tgccaacgcc acatcaattt
    14881 gctcacgcat tttgtctaat gccggcacca cacccgaagt ggaaagcgtg acacgacgtt
    14941 tagataagcc gtaagcaaaa tcatctaaca tgatttccat tgccggaatc acgttattca
    15001 tattcagcaa tggttcaccc attcccatca tgaccacatt ggtaatcggg cgaacaccgg
    15061 tcacgccaaa attgccgata attttagaag cacgccacac ttgcccgata atttccgcca
    15121 cgcttaagtt acggttaaag ccttgttgag cggttgagca gaaggtacaa gccaaggcac
    15181 agcctacttg tgaagaaaca cacagggttg cacggtcatc ttcaggaata tataccgtct
    15241 cgatttgctg atcgcccact tgcattgccc atttaatcgt accgtctgca gaacgttgtt
    15301 ctaccgccac ttcaggggct ttaatttcgg caatacgctt taatttttca cgcaatactt
    15361 tattgatatt gctcatatta tcgaaatttt cttcgccaaa atggtagatc catttcatta
    15421 gctggtcggc acggaaaggt ttttccccca tttctgcaaa cagttctcgc atttcttgac
    15481 gattaagatt taataaattg attttttctt ttggttgttc agcagaccca cagcctgcat
    15541 tggtaatcag ttctgacata aagttgaaag cctcgttatt acacggatta cggcaaaagg
    15601 ttagggcaaa tgcccttatt ttgcgattgg attttaaagc gtgcaatttt actggctctt
    15661 gagggattag gctagtaatt tttaatcaaa agagttgaat tgcctgagta aacttatcaa
    15721 gaataacctt ataaaaacaa gactatttac atcattccca atgttctata gaatagcttt
    15781 cgataattct cattctcaat aacaaaagga ggctaaaaat gaaactatca acaattaaaa
    15841 aagcattggt attaaccttt ggtttaagtg cagcgatggc aatggcagag ccgtttaaag
    15901 tagttaccac ttttaccgtg attcaagata ttgcacaaaa tgtcgccggc gataaagcca
    15961 ctgtggaatc cattactaaa ccgggtgctg aaattcacga ttatcaacca acaccaaaag
    16021 acattgtaaa agctcaaaag gcagacttag tgttatggaa cggtatgaac cttgagcgtt
    16081 ggtttgaacg tttctttgaa aatgtaaaag gtaaaccggc ggttgtcgtc accgaaggca
    16141 tcaccccaat gccgattacc gaaggtgaat ataaaaatct accaaatccg cacgcttgga
    16201 tgtccaccga taacgcatta atttacattg aaaatattcg tgcggcttta gtaaaatatg
    16261 acccacaaaa cgcagacacc tataatgcca atgcaaaagc ctatgctgaa aaagtaaaag
    16321 gtattgctga gccactccgc caacgtttag cttttattcc tgagcagcaa cgttggttag
    16381 taaccagtga aggggcattt agctatttag cgaaagacta caacttaaaa gaactttatt
    16441 tatgggcaat caatgcggaa gagcaaggtt cgccaaaaca agtgaaaaaa gtgattgatg
    16501 gtgtaaaagc caacaaaatc cctgttgtgt ttagcgaaag taccgtttcc gacaaaccgg
    16561 caaaacaagt ggcgaaagaa accggtgcat tatacggtgg cgtaatttat gtggattcac
    16621 tttcagagaa agatggtccg gttccaacct atttagattt gcttaaagtg actatcggca
    16681 ccattgtcga agggttcgaa cgaagcaaaa aataataaga tttagcaatg acaactgttt
    16741 cagcctccat ttccgtcgaa aatttatcgg tccgttataa taacggacat atcgctttgc
    16801 aagatgtctc gtttcatctg gaaaacggca caatttgtgc attaatcggc gtgaatggag
    16861 gtggaaaatc cactctgttt aaaagtttaa tggggctggt taagccatta accggctctg
    16921 ttaagctgag tcagctcccg attgctaaag ccctcaaaca aaatttggtt tcctatgtgc
    16981 cacaaagtga agaagtggac tggcaatttc cagtttcggt ttacgatgtg gtaatgatgg
    17041 ggcgatacgg ctatatgaat tttctacgcc gcccaagcca aaccgataag ctcaaagtag
    17101 agcaagcaat gcaacgcctt gatgttttac atctcaaaga caggcaaatc ggcgaacttt
    17161 ccggcgggca aaaaaaacgg gtatttttag cccgtgcctt agctcaagaa agtaaaatca
    17221 tcttacttga tgagcctttc accggtgtgg atgttaaaac cgaaaatgcg attgttgagc
    17281 tacttaaaca gcttaaaaat gaggggcatt tgattttagt ttccacccac aaccttgcca
    17341 gcgtaccaag cttttgcgat caggttttga tggtcaatcg cacgctactg gcaaaaggta
    17401 aaacggaaga aatctttaat aatcaaaatt tagaacgggt gttcggcggt ttattgcatt
    17461 atcaaaagta gtgtttcagc aacaaatcac aaacaaatag ggaggcaaat gcctccctat
    17521 ttttgtatca atgaatctta gaatatgcaa aactgaggaa aaaaatggcc gcttgcacga
    17581 gaataatcgt cggccctgtt gcggcatcaa tatggtagct aagcaacgtg ccgaataccg
    17641 atgaaaatat cgcaacgcct aaggcaatca gtaacatact atcaaaccgc ttagtcagca
    17701 ggtaagcagt gatccccggg gtaatcaaca ttgccaccac taaaatcacg cccaccactt
    17761 gcattgcact gataatagtt aaagcaagca aggaaagtaa accataatgc aataatctaa
    17821 ccggtaagcc tgctactttg gcttgattaa catcaaagca atagagcaag aagtctttgc
    17881 gtttcgccac catcaccaca aaggtaaaga ggcttatgag taaagtctga agcaaatcgc
    17941 ttttggttac gcctaataga ttaccaaata aaatatgggt taaatgctca ccggtattaa
    18001 aggcggtaaa cagcaagatc ccaaaggcaa acatacctga aaacacaatc cccattaccg
    18061 tgtcttcttt caagcgacta ttttctttta aatagcccac cgataaggca cagcctaatc
    18121 ctgaaaaaaa cgcccctatt gagagtggta acccggcaag tgaggaaatc accacaccgg
    18181 gcagaacagc gtgcgaaatt gcatcgccca ttaaagacca gcctttcagc accagatagc
    18241 aagataaaac cgcactcacg ccagcaacta aaattgcggt aattaacgca atttgcataa
    18301 attcaaattg caacggctct aaaatccaat taagcatttt gaacctccac acggtgcaat
    18361 ttccgctttt gagtaatcaa gccatattta ggagcaaagc agaatgcgaa taagaaaatt
    18421 aaggtttgta agcacacaat taccgcaccg gttgcaccgt ctaaaaagta gcttaaatat
    18481 gccccaatcc ctgatgtcag cgtgccgata agcattgcga tcagcactaa tctagggaaa
    18541 cggtcggtaa gtaaataagc agttgctccc ggcgtgacga ccatcgcaat aaccaaaatt
    18601 gccccaaccg tttgcagagc cactactaca caggcactta ataaggtaaa gaaaatcact
    18661 ttatagcgta atggcgaaag cccgactgac aatgcgtggt gttcgtcaaa aaagacgagt
    18721 agcaaatcct tccaaaatag gattaaaccc agtaacgaaa tcgcaataat cagtaccacc
    18781 tgccacaagt catcatcagc aatgcctaaa atatttccca ttacaatcga attaacatca
    18841 accgatgttg gattgaggga aacaataaac ataccaagcg caaaaaaagt cgtgaaaata
    18901 aagccgataa ccgcatcttc tttaattttc gtcaggcttt ttacccaaag aatcgataat
    18961 gccgccaaaa tcccggaaaa aaacgcacca aaggcgtagg gcaattttag tgcataagcg
    19021 atggcaacac ctggcactac agcgtgtgat aaggcatcac caatcaaaga ccagcctttt
    19081 aacatcaaaa aggcggaaag aaaagcacaa actaacccca cgcccatact gacaaaaatc
    19141 gctttttgca tatagttgta ggtaaaaggc tctaataaaa gatcaagcat aaaatcctaa
    19201 caagttgttg ataatagttc gcatatacta tcatataaac tccttttctt gtatggatta
    19261 aacttaaata tctactttaa attgataagg atcaggatag taataacgaa aaccaagctc
    19321 ttggcaaatt ttgtctgctt ttatcagcct tacaagcggt tgattttctt cagaaaaatg
    19381 caaatccgct aacccaaaac gttttgccat ttcactataa taatctttac ggcttgggtg
    19441 ctgttcggca cataaatgaa aaatacgctg cccgttcggc tgctccaaca gcaatgaaat
    19501 ggctgcaata cagtcctcta aatgcactaa attgaccggc tgccctgctc cactcaagtt
    19561 ctgttttccc gccaaataaa aaaccgggtg ccgttttttg cccaccaagc cagctaaacg
    19621 caaaatatca caatgaattg gctggctcag caaccaattt tccattttta ctaataggtt
    19681 agcagaatca accactgcat tttcatcaaa aacgcccgat ttcattggca aaaccgaagt
    19741 cgagctggtg aaaaccagat gtttcaagcc cttttctatt gcaagcgaaa ctaatgtttg
    19801 aataccgcaa ccgtaatttt cagggcgaat ttggctaagg ggcaaggcaa tcactaacgc
    19861 atcagcagca agtaattgct cgatttcgct tgtcactgct aacgcattta aatctagagg
    19921 ataacattct atcgctaatt ctccgaccgt tcgcttagta cctgcaacac gccagccctt
    19981 attttgtaat gcttcagcta aaggtagtcc gagccagcca agccctaaaa tggtaatttt
    20041 tttcatcatt tacgtttctc aagattaaaa ttggaatgag gggaaattca ggtacaaaaa
    20101 aagcacctcg tggtgctatc tattttattt cta
//
LOCUS       DANLYI010000023        19899 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.023, whole genome shotgun sequence.
ACCESSION   DANLYI010000023 DANLYI010000000
VERSION     DANLYI010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 19899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19899
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.023"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            complement(<1..398)
                     /locus_tag="PXD81_000632"
     CDS             complement(<1..398)
                     /locus_tag="PXD81_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HDL5536687.1"
                     /translation="MKKYNIVFSADKNYLPYLEIALKSLLAHHNHLSIFILSTGDISH
                     HWIETLRPYLENRYSTLRLGYLDSNNLANFNSNGYITNSTYLRYYIEELFEHSDSPYW
                     IYLDCDLVVNGNITDPFTNLDFSFGSIAAIA"
     gene            complement(385..567)
                     /locus_tag="PXD81_000633"
     CDS             complement(385..567)
                     /locus_tag="PXD81_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1016 family protein"
                     /protein_id="HDL5536688.1"
                     /translation="MKIMQLTSNQYINDIKHILLSARQKAYQAVNSAMVEAYWKIGER
                     IVQEEKNMVKDQNEKV"
     gene            complement(564..881)
                     /locus_tag="PXD81_000634"
     CDS             complement(564..881)
                     /locus_tag="PXD81_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5536689.1"
                     /translation="MKLLVSHFHQGEGKFPTFSAGTAVRLGSECVNYPNWFACEIDGW
                     KTFVPSHFISEDKLICDYNPTELVAAQGERVEFLELHYQWAIVKRENEIGWLPCEILR
                     VIG"
     gene            complement(881..2083)
                     /gene="coaBC"
                     /locus_tag="PXD81_000635"
     CDS             complement(881..2083)
                     /gene="coaBC"
                     /locus_tag="PXD81_000635"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005602882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HDL5536690.1"
                     /translation="MLQNKKILVGISGGIAAYKTIEFIRHLKKANAEVRVVLTPAAEA
                     FVTPLTLQAISGNAVSTSLLDPQAELAMGHIELAKWADLIVIAPASADFIARLRMGMG
                     NDLLSTLCLATASPILLAPAMNQQMYKQIAVQENLSVLVNRGVQLIGPNSGFQACGDV
                     GAGRMSEPEEIYQAVLAHFSNNQDLEGLNVTITAGPTREAIDPVRYISNHSSGKMGYA
                     IANAFAERGAKVTLISGPVNLPTPKNVERINVESAVEMEQQAVKFAQKSAIFIGCAAV
                     ADYRMAAVSDQKIKKTDANDELTLKLVKNPDIIANVANLAENRPFVVGFAAETQNVAE
                     YAKSKLERKNLDMICANDVSGGQVFGQDQNALHLFWKNGEKTLPLADKSKLAESLVQE
                     IVKHYREK"
     gene            2233..2970
                     /gene="radC"
                     /locus_tag="PXD81_000636"
     CDS             2233..2970
                     /gene="radC"
                     /locus_tag="PXD81_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HDL5536691.1"
                     /translation="MEEKMNDFFDEDSQEPTMMPREKLLTQGAASLTDSELLAIFLRT
                     GTKAVPVLALAESVLMAFGSLRQLLNANIDDFCKIYGLGKTKYIQLQASKEMTKRYLA
                     QQMEFSEMIQAPYMAIMYFQTELEEEEREVFMVLFLDNQNRLIYKEKMFFGTINQTAV
                     HPREIIKRALKYNAAAIIVAHNHPSGSCLPSESDRSLTKKIEMACELVDIRFVDHIIV
                     GKGDYFSFEEEKLEMKEHSFLQISDRK"
     gene            3202..3438
                     /gene="rpmB"
                     /locus_tag="PXD81_000637"
     CDS             3202..3438
                     /gene="rpmB"
                     /locus_tag="PXD81_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_004997628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HDL5536692.1"
                     /translation="MSRVCQVTGKRPAVGNNRSHAMNATKRRFLPNLHTHRFWVESEN
                     RFVTLRLTAKGMRIIDKKGIDAVLAEIRARGEKI"
     gene            3450..3620
                     /gene="rpmG"
                     /locus_tag="PXD81_000638"
     CDS             3450..3620
                     /gene="rpmG"
                     /locus_tag="PXD81_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005624479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HDL5536693.1"
                     /translation="MAAKGAREKIRLVSTAETGHFYTTDKNKRNMPEKMEIKKFDPVV
                     RKHVIYKEAKIK"
     gene            complement(3701..4456)
                     /locus_tag="PXD81_000639"
     CDS             complement(3701..4456)
                     /locus_tag="PXD81_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDL5536694.1"
                     /translation="MSKNRLEQILLFLKNHNIATVEQLVKVTEASPATIRRDLIKLDE
                     EGVVIRTHGGVSLNQFIPFQPTTNEKQFKQIVEKERIAEYAVSLISPGNSILLDAGTT
                     TLCIAKKIAHMPLRVITTDLHIALLLSEYKQIELIITGGRVDNSSQSCIGNHSLTLLE
                     NINPDIAFVSCNSWSLEKGVTAPTEEKANLKRMLQQNAKQKVLVADSSKYGKYSLFRA
                     TQLDNFTQIITDKGLPIEYSQALSSNDFKLSLV"
     gene            complement(4564..5790)
                     /locus_tag="PXD81_000640"
     CDS             complement(4564..5790)
                     /locus_tag="PXD81_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HDL5536695.1"
                     /translation="MKETLLVIADDLTGANDTAVMFAESGFDTVLKTKVSALAQIHPD
                     KAQVISVSTDSRAIGEKAKELTQIAISNAIQNSIGQIYLKIDSTMRGSVKYQIEGAIK
                     AWAGLYPDVKAIICPAYPEMGRTIEAGHLYVNGVPVNETASGKDPICPVHSSSMQKLL
                     PDAQVIPCDTVDNLQASIENSEYKQIVIDAKTHEDLSMIAQVINHIGNKIIPVGSAGL
                     AQKLKNIQNQQQPERNLKLGRALIVVTSIHETSQLQVDEYISTLGGKSIVFNPSPSQL
                     INYSQSEHSLKLQLNALIRFSRDHVIIRANPAKVVNAKSANITETSKEISQHLADLSK
                     FCLDNEKFDSLILFGGDGAAALLDQMNVTEMNVQYAVVPGVPLCSIEKGNYSGITVMT
                     KSGGFGNNTLLSNIMN"
     gene            complement(5787..6785)
                     /locus_tag="PXD81_000641"
     CDS             complement(5787..6785)
                     /locus_tag="PXD81_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate permease"
                     /protein_id="HDL5536696.1"
                     /translation="MSTQTSKSIVPIYDGMNKIPGGAMVIPLILGSIIGTFFPEFLNL
                     GSFTTALFKSSAGPLIALLIFATGMQITVRSSGPVLAHTGVVLLFKTIIPALLVVILG
                     LWVGNDGVMGVSLLAMLTVVANSNGGIWLAFTGKYGDYRDKGAYVASAVNDGPFFVLL
                     FLGVSGLADIPLSLMLAAVIPLIIGMVIGNLDPKWTELMKPTGAIVIPFFAFALGTGI
                     NLQSIITGGATGIILGIVASLFTGFLVFIGYKTILRRGNQSGIGFAAGTTAGNAIITP
                     EIVAQADPTLAPFVQTATAQIAAAVLVSAILAPLFAAWVLKRQGGLEQDTIKGENL"
     gene            complement(6796..7797)
                     /gene="pdxA"
                     /locus_tag="PXD81_000642"
     CDS             complement(6796..7797)
                     /gene="pdxA"
                     /locus_tag="PXD81_000642"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011609079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HDL5536697.1"
                     /translation="MKPILGITMGDAAGIGPEVIIKALANQRIYELAHPVVIGDRKII
                     QRALPIVHSELKLRILNNLDNFVAEYGVIDILDLNNLPEDLPFAQVDPRAGKAAYEYI
                     EEAVNLAMGGKIHAIVTAPLNKEALHAGGKPFPGHTEILATLSNTKDYSMMLTSEKLN
                     VIHVTTHVQLRKACDLITKERVATVIDLAEENARILGFKEPRIAVAGLNPHSGENGMF
                     GDEDQKEIVPAIQEAQAKGFNVTGPVPPDTLFHRAANLNEFDIVVVMYHDQGHIPIKL
                     LGFDTGVNVTVGLPFIRTSVDHGTAFPIAGKGTADSRSMTEALYLGAKMANIKYATH"
     gene            8231..9595
                     /locus_tag="PXD81_000643"
     CDS             8231..9595
                     /locus_tag="PXD81_000643"
                     /EC_number="2.6.1.76"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005820549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HDL5536698.1"
                     /translation="MTIKTPIQAVLASNQYFLDRQDAMESNVRSYPRKLPFAYQKAQG
                     VWVTDVEGNEYLDFLAGAGTLALGHNHPKLIQAVKDVLDSGLPLHSLDITTPLKDAFT
                     EELLSFFPKDKYILQFTGPSGADANEAAIKLAKTYTGRGNVIAFSGGFHGMTHGALSL
                     TGNLSAKNAVQGLMPGVQFMPYPHEYRCPFGIGGEAGAKAVEHYFENFIEDVESGVVK
                     PAAVILEAIQGEGGVVPAPISFLQKVREVTQKHGILMIVDEVQAGFCRSGKMFAFEHA
                     GIEPDIVVMSKAIGGSLPLAVLAIKKEFDAWQPAGHTGTFRGNQLAMATGYVSLKIMR
                     EENLAKNAQERGEYLTNALRELSKELPCIGNVRGKGLMMGIDIVDERQPKDATGAYPR
                     DCDLAVAIQKYCFKNKLLLERGGRGGNVVRVLCAVNITQAECEEFIKRFKQSVAEAVK
                     AVRG"
     gene            9685..11220
                     /gene="ddc"
                     /locus_tag="PXD81_000644"
     CDS             9685..11220
                     /gene="ddc"
                     /locus_tag="PXD81_000644"
                     /EC_number="4.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005693296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2,4-diaminobutyrate decarboxylase"
                     /protein_id="HDL5536699.1"
                     /translation="MSNLEKHRQSLFCSDPKSIADYESAMNNAVKAVSDWLKEEKMYT
                     GGSIKELRAKINGFNPTQAGVGVQKSLEHLVEIFLKPSLKVHHPHSLAHLHCPTMVTS
                     QIAEVLINATNQSMDSWDQSPAGSIMEEHLIDWLRQKVGYGKGTSGVFTSGGTQSNLM
                     GVLLARDWAIANHWKKADGSEWSVQADGIPADAMQKVKVVCSENAHFSVQKNMAMMGM
                     GFQSVVTVPSNANAQMDVEALAKTLADLKAEGKIVACIVATAGTTDAGAIDDLKAIRK
                     LADEHQAWVHVDAAWGGALLLSKDFRHFLDGIELTDSITLDFHKHFFQSISCGAFLLK
                     DEANYRFIDYKADYLNSEYDEEHGVPNLVSKSLQTTRRFDALKLWFTVEALGEELYAS
                     MIDHGVYLSKEVEAYINATDGLEMLVPAQFASVLFRVVPQGYPAEFVDTLNQNVADEL
                     FARGEANIGVTKVGEHQSLKMTTLSPIATLENVRALLAQVLAEADRIKDSIVNGTYVP
                     PID"
     gene            11323..11685
                     /locus_tag="PXD81_000645"
     CDS             11323..11685
                     /locus_tag="PXD81_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015586988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NirD/YgiW/YdeI family stress tolerance protein"
                     /protein_id="HDL5536700.1"
                     /translation="MKKVLGLTLMAMALSACNSTASNGSSQANTGVAPANVISVKQAL
                     AAADDSYVTVEGTLLNKVGDETYLFKDATGQIRVEIDDEVWAGQHVGSEGNKVRLYGE
                     IDKGFNKTELDVKTLTVL"
     gene            complement(11775..12362)
                     /locus_tag="PXD81_000646"
     CDS             complement(11775..12362)
                     /locus_tag="PXD81_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HDL5536701.1"
                     /translation="MNHLIIYAHPNPSSFNHAILEKTIAASVPNKVVVRDLYQLNFKP
                     ILDWAEFQGTLSKHYEADVAIEHQYWLEADVITLIYPLWWMGFPAILKGYLDRVLSYD
                     FAYRNGEIESIGLLTGKKMQQFVTLGNSNEKYERKGFLAAFEHTLGKGLFEFCGIRDV
                     KMHYFGNIGLKETDYTAILQQITASCSTILKEQAV"
     gene            12538..13086
                     /gene="napF"
                     /locus_tag="PXD81_000647"
     CDS             12538..13086
                     /gene="napF"
                     /locus_tag="PXD81_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HDL5536702.1"
                     /translation="MTDYPNQSLPRRSFLRGQFLDSLKSQKVSQQGYEPIRPPWANLA
                     NFLEKCTACGNCIQACETQIIVKGAAGYPEIDFSRGECTFCEKCVHSCEAEVFRAVSE
                     QPWSHKIEIQAGCLLKLGTECRSCGDSCEMRVIRFRPSLGGIAEMVLNLESCNGCGAC
                     LSVCPTSAIKIQPLQTQANRDE"
     gene            13079..13345
                     /locus_tag="PXD81_000648"
     CDS             13079..13345
                     /locus_tag="PXD81_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HDL5536703.1"
                     /translation="MSEKLSEQENWYVCSLVVQAKPEKLEEVKAEILKIPHTEIHGEK
                     TDEGKLVVTIESNVHLALSDRIDQVRDIKGVIVVSLISNYIDEQ"
     gene            13384..15882
                     /gene="napA"
                     /locus_tag="PXD81_000649"
     CDS             13384..15882
                     /gene="napA"
                     /locus_tag="PXD81_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005566990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase catalytic subunit NapA"
                     /protein_id="HDL5536704.1"
                     /translation="MELNRRDFMKANAAVAAAAAAGIMIPVKNVQADDTGIRWDKAPC
                     RYCGTGCSVLVGTKDGKVVATQGDPDAEVNRGLNCIKGYFLSKMMYGADRMQEPMLRM
                     KDGKFDKNGDFTPVSWDQAFSIMAEKIKTTIKNDGPNAVGMFSSGQTTIFEGYAKVKL
                     WKGGFRSNTIDPNARHCMASAAVAFMRTFGMDEPMGCYNDIEKTDAFVLWGSNMAEMH
                     PILWSRISDRRLSDNKVKVVVMSTYEHRSFELADTPIIFKPHSDLAILNYIANYIIQN
                     DKVNWDFVNKHTKFKRGETNIGYGLRPEHKLQQNTNAKTAGKMYDSDFEEFKKIVAPY
                     TLEEAHRISGVPKDQLETLAKLYADPNQKLVSFWTMGFNQHTRGVWVNHMMYNVHLLT
                     GKIALEGCGPFSLTGQPSACGTAREVGTFIHRLPADMVVTNPKHREIVENSWKLPAGA
                     IPDVPGFHATAQSRALKDGKMKFLWQMCTNNMQGGPNINEEIFPGWRNPETFIVVSDP
                     YPSVSAVAADLILPTCMWVEKEGAYGNAERRTQFWRQQVKGPANARSDTWQLVEFSKY
                     FKVEEVWPEELLAKAPELRGKTLYEILYRNGKVDSYQVPTNIPGYMNDEADHFGYYIQ
                     KGLFEEYAEFGRGHGHDLADFDTYHKVRGLRWPVVKDEKTGEYKETLWRYREGYDPYV
                     KAGEEVAFYGNADKKAVILGVPYEPPAEAPDEEYDLWFCTGRVLEHWHTGTMTRRVPE
                     LHKAFPTNLVWMHPNDAKKRGLRHGDKVKVISRRGEYVSNLDTRGRNKCPEGLIYSTF
                     FDAGQLINKVTLDATDPISFETDFKKCAVKVVKA"
     gene            15971..16876
                     /gene="napG"
                     /locus_tag="PXD81_000650"
     CDS             15971..16876
                     /gene="napG"
                     /locus_tag="PXD81_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HDL5536705.1"
                     /translation="MKMDPNRRQFLKNATRTAAGVCGVGMILGLQQQQSFARQGVALR
                     PPGALPEQDFLSACTRCGQCVQACPYDMLHLASLISPVEAGTPYFIARDKPCEMCLDI
                     PCMNACPTGALSEELKDIDDARMGLAVLLDHETCLNWQGLRCDVCYRVCPLIDKAITL
                     EKVHNDRTSIHAKLIPTVHSDACTGCGKCEQACVLEEAAIKVLPMDLAKGLLGRHYRL
                     GWQEKQNAGKSLIEDVHPEGLRPAYDARMPEGLHEPVYQHMEVKPDVKVATPSRATQN
                     YVPNSTTVEAPEHFPDLDLNLKGVK"
     gene            16876..17754
                     /gene="napH"
                     /locus_tag="PXD81_000651"
     CDS             16876..17754
                     /gene="napH"
                     /locus_tag="PXD81_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HDL5536706.1"
                     /translation="MAANSPKNAGLEARQKLGWWHAYRFLILRRLSQLSIILMFLSGP
                     LWNVWILKGNYSGSLLLDTVPMTDPLMTAEVLATGYRPEWTVLVGALIMVAFYGIVGS
                     KLFCSWVCPMNMVTDTAAWLRRKLGIRQTAKIPRNLRYVILAVILVGSAISGTLLWEW
                     INPVAALGRVFVFGLGATLWLVLVVFLFDLLVAEHGWCGHLCPIGATYALIGAKSLIK
                     VKVVDRNRCDNCMDCYNVCPEPQVLRVPLHGKPEESTVILSKDCITCGRCIDVCAENV
                     FAFGMRFQGSIDVKNI"
     gene            17800..18228
                     /locus_tag="PXD81_000652"
     CDS             17800..18228
                     /locus_tag="PXD81_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HDL5536707.1"
                     /translation="MRKYLAFILAVMSGFAMAEAPKLSGSIDETTESVAPGFHNPQKD
                     VGTIPTTFPFQPPLVPHSIRGLQVSKNVNHCLGCHAIEPSKTTGATRLPPSHFTDRDG
                     KVYENESPRRYFCLQCHVQQTDVNPIIQNKFESVRESQAK"
     gene            18239..18847
                     /locus_tag="PXD81_000653"
     CDS             18239..18847
                     /locus_tag="PXD81_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HDL5536708.1"
                     /translation="MLRRFCNWLRSPSKMAIGAVILISALGGIFAWSGFNAGLAMTNT
                     EEFCSDCHMNDVVPEYRASAHYSNRSGVKAICSDCHVPHEFFPKWQRKIIAAKEVYAH
                     FTGKVDTKEKFEAHRGEMAEREWARMKANSSQECRNCHNFDDMDFTQQKNIAQQMHAL
                     AQEQGKTCIDCHKGIAHNLPHMEAIQKNFIPDDLLKKAEEKK"
     gene            complement(18909..19751)
                     /gene="cpdA"
                     /locus_tag="PXD81_000654"
     CDS             complement(18909..19751)
                     /gene="cpdA"
                     /locus_tag="PXD81_000654"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HDL5536709.1"
                     /translation="MDAKRMHEYIKLPVTGVIKLLQITDPHLFSNPEETLLNVKTVKS
                     FSAVIEQINKQAKQYFDLVLATGDLIQDNNIAGYHYFAQITNSLNSPIVWLEGNHDVQ
                     PSMSEILAQYKHILPHKQILLGEQWQLLMLNTQVVGMPYGELSYSQLKWLEEKLAQYP
                     QRFTLVALHHNILPTNSAWLDQHSLKNSNELSEIFAKFKNVKAIVHGHIHQQVDDIWH
                     GIPIYATPSTCIQFKPNCDQFTLDLQPQGWREIELHENGTIETVVKRLNSNDFLPNVN
                     AIGY"
BASE COUNT         5912 a         3893 c         4272 g         5822 t
ORIGIN      
        1 gcaattgcag caatacttcc aaaagaaaaa tctaggtttg taaatggatc cgtaatattt
       61 ccattaacca caagatcgca atctaaataa atccaatatg ggctatcgct gtgttcaaat
      121 agttcttcaa tataatatcg taggtaagtt gaattagtaa tatagccgtt ggagttaaaa
      181 tttgctaaat tatttgaatc taaatagcca agtcttaatg tggaatatct attttctaaa
      241 taggggcgta atgtttctat ccagtggtga gaaatatcgc cagtactcaa tataaaaata
      301 gataaatgat tatgatgagc taatagtgat tttaaggcaa tctctaaata tggcaggtag
      361 tttttatctg ctgaaaatac aatattatac tttttcattt tgatctttga ccatattttt
      421 ctcttcttgc acaattcgtt caccgatttt ccaataggct tcgaccattg ccgaattcac
      481 ggcttgatac gccttttggc gagcagataa aagaatatgt ttaatgtcgt tgatatactg
      541 atttgacgta agttgcataa ttttcatcct attaccctta agatttcaca aggtagccag
      601 ccaatttcgt tttctctttt gacaattgcc cattggtaat gtagttcaag gaattctact
      661 ctctcgcctt gtgctgcaac aagttccgtt gggttataat cgcatatcaa tttatcttcg
      721 ctaataaaat ggcttggcac aaaggttttc caaccgtcaa tttcacaggc aaaccagttt
      781 gggtagttta cacattcact ccctaatcga acggcagtac cagctgagaa tgtaggaaat
      841 ttcccttcgc cttgatgaaa atggctaacc agtaatttca ttatttttct cgataatgtt
      901 ttacaatttc ctgtaccaaa ctttctgcca atttgctttt atccgccaga ggaagcgttt
      961 tttcaccatt tttccagaac aaatgcaacg cattttgatc ctgcccgaac acttgtccgc
     1021 ctgagacatc attggcacaa atcatatcca gatttttgcg ttcgagtttc gatttagcgt
     1081 actccgccac attttgcgtt tctgcggcaa aaccaaccac aaaagggcgg ttttcggcaa
     1141 gattggcgac gttagcgata atatcggggt tttttaccaa ttttagcgtg agttcatcat
     1201 tggcatcggt tttcttaatt ttttggtcag agactgccgc cattcggtaa tcggcaaccg
     1261 ctgcacagcc aataaaaatc gcagattttt gggcaaattt gaccgcttgt tgctccatct
     1321 caaccgcaga ttccacatta attcgttcca catttttcgg cgttggtaaa ttcaccggac
     1381 cgctaatcaa cgtaaccttt gctccacgtt cggcaaaggc attagcaatc gcgtagccca
     1441 ttttgccgga gctgtggttg ctgatataac gcacagggtc aatcgcctca cgagtcggtc
     1501 cggcggtaat ggttacattt aagccctcta aatcttgatt gttggagaaa tgagccaaca
     1561 ccgcttggta aatttcttca ggctcagaca ttctgccagc ccccacatca ccacaggctt
     1621 ggaagccact attcgggccg atgagctgaa cgccacgatt gactaaaaca gacagatttt
     1681 cttgcaccgc aatctgtttg tacatttgtt gattcattgc cggtgcaagc aaaatcgggc
     1741 ttgcagtcgc aaggcagaga gtggaaagta aatcgttgcc cattcccata cgcaggcgag
     1801 caataaaatc ggcacttgcc ggggcgataa cgattaaatc cgcccatttt gccagctcaa
     1861 tatgccccat cgccagttcg gcttgtgggt cgagtagtga agtggaaacc gcattgccgg
     1921 aaatcgcttg taaggtcaaa ggcgttacaa aggcttctgc tgctggggtt agcactacac
     1981 gcacttccgc atttgccttt tttaagtggc ggataaattc aatggttttg taggctgcaa
     2041 tcccaccgga aatgccgact aaaatttttt tgttttgtaa catctttact atccttaaag
     2101 cgtggattta ttttgcatat tttagcggaa taaggagttg ctcacaactt cactattttt
     2161 gcgatcttgc tcgaaaaatt gaataaatcc ctttgtctgc aaaggttggc tcttttatgc
     2221 ttaactaaat tgatggagga aaaaatgaac gatttttttg atgaagattc tcaagagcca
     2281 acaatgatgc cgcgtgagaa attattaaca cagggggcag cctcattaac cgattccgaa
     2341 ttattagcga tatttttacg gactggtacg aaagctgtgc ctgtgctggc attggcagag
     2401 agtgtgttga tggcatttgg ctcgttacgc caattactga atgcaaatat tgatgatttt
     2461 tgcaaaattt acggtttagg caaaaccaaa tacattcagt tacaagcctc taaagagatg
     2521 accaaacgct atctggctca acaaatggaa tttagcgaga tgattcaagc cccttatatg
     2581 gcgattatgt atttccaaac cgagttggag gaagaagaac gggaagtttt tatggtgctg
     2641 tttttggaca accaaaaccg attgatttat aaagaaaaaa tgttcttcgg caccattaat
     2701 caaactgctg ttcacccccg agaaattatt aaaagagcgt taaaatataa tgccgcggcg
     2761 attattgtgg ctcataatca cccctcaggt tcttgtctgc cgagtgagtc ggacagaagt
     2821 ttaactaaaa agatcgaaat ggcgtgcgaa ttggttgata ttcgctttgt ggatcatatt
     2881 attgtcggca aaggggacta tttttcgttc gaggaagaga aattagagat gaaggagcat
     2941 tcatttttgc aaatttctga tagaaaatga ccgcttgctt tatgtaacct ttaacatttc
     3001 tcctgaaaca gtaaaagata cgcatataaa ttctacttga atataacaaa tgaaatcggt
     3061 ataatcggca gtctttatta catagactgt ggtcgggtta atgcctgacg aggcaatcaa
     3121 aatcggaggg gttggttgag caacgccttt tgtgagatcg cccgaaccat ttttaagctt
     3181 aaaattattg gagaaattca aatgtcaaga gtttgccaag taaccggcaa gcgtccagca
     3241 gttggtaaca accgctcaca cgcaatgaat gcgactaaac gtcgtttctt accaaacctt
     3301 cacactcacc gtttctgggt tgagagcgaa aaccgtttcg taaccttacg tttaactgcg
     3361 aaaggtatgc gtattattga taaaaaaggc attgatgcag tattagctga aatccgtgct
     3421 cgtggcgaaa aaatctaagg agctaaaaaa tggcagctaa aggcgctcgt gagaaaatcc
     3481 gtttagtttc tacagcagaa actggtcact tctacacaac agataaaaac aaacgtaata
     3541 tgcctgaaaa aatggaaatc aaaaaatttg atccagttgt gcgtaaacac gttatctaca
     3601 aagaagcaaa aattaaataa ttttgtttta agatttaaaa cccagcgaat gctgggtttt
     3661 tcatatacaa aaaattagtg aaaaaagacc gcttgtatga ttaaactaat gatagcttaa
     3721 aatcattact tgacaaggct tggctatatt caataggtag tcctttatct gtgataattt
     3781 gtgtaaagtt atcgagttga gttgcacgaa ataaagagta ttttccatat ttgctgctat
     3841 cggcaaccag tactttttgt ttggcgtttt gttggagcat tctttttaaa ttagctttct
     3901 cttctgtagg tgctgtaact cctttttcta agctccaaga attacagctc acaaaagcaa
     3961 tatcaggatt aatattttct aataaagtta aactatgatt accaatacaa gattgactac
     4021 tattatctac tcgtccccct gtaataatta attctatttg cttatattca gagagtaata
     4081 aagcaatatg tagatcagtt gtaatcactc gaagtggcat atgagcgatt tttttcgcaa
     4141 tgcatagcgt tgtagtgcca gcatctaata aaatagaatt tcccggtgaa ataagcgaaa
     4201 cggcatattc agctatgcgt tctttttcga caatttgctt aaattgcttc tcatttgttg
     4261 ttggttgaaa cggtataaat tggtttaagc tgacccctcc gtgtgtgcga atcacgacgc
     4321 cttcttcatc taattttatt aaatcacggc ggatagttgc aggagaagcc tcagtcactt
     4381 ttaccagttg ctctacggta gcaatattgt ggttttttag gaatagtaaa atttgttcta
     4441 gacggttttt agacatataa actctctaat ttttaactgc ttttactata agtaattttt
     4501 taaaaataag atagttaaaa ttaaagaacc taaaacattg ttgctttagg ttctttgttg
     4561 ttactagttc attatattgc ttaagagtgt gttgtttcca aatcctccag atttagtcat
     4621 tacagtgatg cctgagtagt tgcccttttc aatggagcat aaaggaacgc ccggtactac
     4681 cgcatattgt acattcatct ctgttacatt catttgatct aataatgctg ctgcaccatc
     4741 tccaccaaat aatataaggc tatcaaattt ttcattatca aggcagaatt tacttaaatc
     4801 agctaaatgt tgagaaattt ctttagaagt ttccgtgata ttggcagatt ttgcatttac
     4861 aacttttgca gggtttgccc gaataataac atgatctcta ctaaaacgta ttagagcgtt
     4921 taattgtagt ttcagtgaat gttctgattg cgagtagtta atcaactgtg aaggcgaagg
     4981 attaaataca atagattttc ctcctaaagt tgaaatatat tcatcaactt gcagttggct
     5041 tgtttcgtga attgaggtta cgacaattaa tgctctacct aatttaagat ttcgctctgg
     5101 ttgttgttga ttttggatat ttttcaactt ctgtgccaat ccggctgagc caacaggaat
     5161 aattttatta cctatgtgat ttatgacttg ggcaatcatt gagagatctt cgtgcgtttt
     5221 agcatcaata acgatctgtt tatattcact attttcaata gatgcctgca aattatcgac
     5281 cgtatcacac gggattactt gagcatcagg caatagtttt tgcatagaag atgaatgcac
     5341 agggcagata ggatcttttc ctgaagcagt ttcatttaca ggaacaccat taacatataa
     5401 atgaccagct tctatagttc ttcccatttc tggatatgca ggacaaataa ttgctttaac
     5461 atcgggatat aaaccagccc aagctttaat agcaccttca atctgatatt taacagagcc
     5521 tctcattgtg gaatcaattt taaggtaaat ttgcccgata ctattttgaa tagcattgct
     5581 aattgcaatt tgagtcagct cttttgcttt ctctccaatt gctcttgaat ctgtagaaac
     5641 agaaattact tgggctttat caggatgaat ttgggctaat gccgaaactt tagtttttaa
     5701 gacagtgtca aatcctgatt cagcaaacat aaccgcggta tcatttgcac cagtaaggtc
     5761 atcagcaata actaagagtg tctctttcat agattttctc cttttatagt gtcttgttct
     5821 aagccacctt gacgttttaa aacccacgct gcaaataatg gagctaagat tgctgaaact
     5881 aatacagctg cggcaatctg tgcagtcgca gtttgaacaa aaggtgctaa tgtagggtct
     5941 gcttgagcaa caatttcagg tgtaataatg gcatttccag cagtagttcc tgctgcaaac
     6001 ccgattcctg attgattacc acgtcttaaa attgttttgt aaccgataaa tacaagaaaa
     6061 cctgtaaaga gagaagctac gatacctaaa ataattcctg ttgctcctcc agtaataata
     6121 gattgcagat tgattcctgt tcctaatgca aaagcaaaga aaggaatgac tattgcacct
     6181 gttggtttca ttaactctgt ccatttagga tctaagttac caataaccat accaataata
     6241 agaggaataa cagctgcaag cattaatgaa agaggaatat ctgctaatcc tgatacacct
     6301 aaaaatagta aaacaaagaa aggtccatca ttaacagccg aagcgacata agctccttta
     6361 tctctataat caccatattt ccctgtaaac gctaaccaaa taccaccatt tgaatttgca
     6421 acaacagtta gcatagctaa taaagaaacg cccataacac catcattacc tacccataat
     6481 cctaaaatca caactaaaag agcgggaata atagttttaa atagtaatac aacaccggta
     6541 tgggcaagta caggtccaga acttctcacg gtaatttgca tacctgtagc gaaaattaat
     6601 agtgcaataa gtggtcctgc tgatgatttg aataaagccg tcgtaaatga acctaaattt
     6661 aaaaattcag gaaagaatgt accaataata gaaccaagaa ttagcggtat taccattgca
     6721 ccgccaggaa ttttattcat accatcatat atcgggacga tggatttaga tgtttgagta
     6781 gacatattaa tctccttagt gtgtcgcata tttgatattt gccatttttg cacccaaata
     6841 gagtgcttca gtcatactac gactatctgc tgttcctttg cctgctattg ggaaagcagt
     6901 tccgtgatcg acagatgttc gaataaatgg aagaccgaca gtaacgttta cacctgtatc
     6961 aaagcctaat aatttgatag ggatatgccc ttgatcgtga tacatcacaa caacaatatc
     7021 gaattcattt aggttagctg cacggtgaaa taaagtatcc ggagggacag gccctgtaac
     7081 attaaaccct ttggcttgag cctcttgaat tgcgggaaca atttcttttt gatcttcatc
     7141 gccaaacatt ccattttcac ctgaatgagg atttaaacca gcgactgcaa tacgaggttc
     7201 tttaaaccca agaatgcgag cattttcttc tgctaaatca ataactgtag caactcgttc
     7261 tttggtaatt aaatcacagg ctttacgaag ttggacgtga gtagtaacgt gaataacatt
     7321 gagtttttca ctggttagca tcattgagta atctttagta ttactgaggg ttgcaaggat
     7381 ctctgtatgg ccaggaaatg gtttaccacc tgcgtgtagg gcttctttat tcagtggtgc
     7441 tgtaacaata gcgtggattt ttcctcccat tgctaaatta actgcctctt caatatattc
     7501 ataagctgct tttccagcac gcggatcaac ttgagcaaaa ggcaaatctt caggcaggtt
     7561 attcaaatct aaaatatcaa taacgccata ttcggctaca aagttatcta gattattgag
     7621 aattcttaat ttcaattctg aatgaacaat aggtaaggct cgttgtataa tttttcgatc
     7681 accgatcaca acagggtgtg ctaactcata aatccgttga ttagctaatg ctttaataat
     7741 aacttctggt ccgataccgg cggcatctcc catcgtaatt cccaatatag gtttcatatt
     7801 tactccttga ttgaaattaa tcattttgaa cgaagtatag cttatatgaa atcataaaag
     7861 tgtgataaac atcacaaaag tgaacttttt taatcttatt gatttaattt aatcaatttt
     7921 aaggtggtaa attggatagg gtatgaaagt gaaaggcatt tattgctaga taaaagcttc
     7981 ccttttcaat aaaaaagagt atcatacgct ctcttaaacg taaccctaca tttaagtctg
     8041 tttccaacct atcccaattt ctttattcct taggggcata tagggtaaaa acaaaaaagc
     8101 tgaaacgctt tttttaataa gtagcgagtc aaatgcccca tggcatctat tcttttagga
     8161 atagaaagct taggctttcc gcaaggcacc cgacttattt ttttcacctc ttgtagaagg
     8221 tttattttca atgacaatta aaactcctat tcaagccgtt cttgcttcaa accaatattt
     8281 tttagatcgc caagatgcaa tggaatcaaa cgtgcgtagc tacccacgta aattaccttt
     8341 tgcttaccaa aaagcacaag gcgtttgggt aactgatgtt gaaggcaatg aatacctcga
     8401 tttcctagca ggtgcaggaa cgcttgcatt aggtcataac caccctaaat taatccaagc
     8461 agttaaagat gtattagaca gtggtttacc tttacactcg ttagatatta cgactccgtt
     8521 aaaagatgct tttacggaag aattactctc tttctttcca aaagataaat atatcctcca
     8581 atttactggc ccgtctggtg cagatgctaa cgaagcggca attaaattag ccaaaaccta
     8641 cactggtcgt ggcaacgtaa ttgctttctc cggtggtttc cacggaatga cccatggtgc
     8701 gttatcgctc accggtaatt taagtgcgaa aaatgcagta caaggcttaa tgccgggcgt
     8761 acagtttatg ccttatccgc acgaataccg ctgtccgttt ggcattggtg gcgaagccgg
     8821 tgcaaaagca gttgaacatt attttgagaa ctttattgaa gatgttgaaa gtggtgtggt
     8881 aaaaccggca gcggtgatcc ttgaagccat tcaaggtgaa ggcggtgtgg ttccggctcc
     8941 aatcagcttc ttacaaaaag tgcgtgaagt cactcaaaaa cacggcatct taatgattgt
     9001 ggacgaagtt caagctggtt tctgccgttc agggaaaatg tttgcctttg aacacgcagg
     9061 cattgagccg gatatcgtag taatgtcaaa agcgattggt ggtagcttac cgcttgcggt
     9121 attagcgatc aaaaaagagt ttgacgcttg gcaaccggca ggtcataccg gtacattccg
     9181 tggtaaccaa ttagcaatgg caaccggcta tgtatcgctt aaaattatgc gtgaagagaa
     9241 tttagcgaaa aacgcacaag agcgtggcga atacttaact aacgcattac gtgaattaag
     9301 caaagaatta ccttgtattg gcaatgtgcg tggtaaaggc ttaatgatgg gaattgatat
     9361 tgttgatgaa cgtcagccaa aagatgcaac aggtgcatac ccgcgtgatt gcgatttagc
     9421 cgttgcaatt caaaaatact gctttaaaaa caaattgtta ttagagcgtg gcggacgtgg
     9481 cggtaatgtg gtgcgtgttc tttgtgccgt gaatattacc caagcagaat gtgaagagtt
     9541 tattaaacgc tttaaacaat ctgttgctga agcagtgaaa gcggtgcgtg gttaatagtt
     9601 agaatggtag gagcgaaaca tttttcgccc taatgtaaaa aggggcgaat atttattcgc
     9661 ccctacacca ataggataga aataatgtca aacctagaaa aacacagaca atccctgttt
     9721 tgtagcgatc caaaatcgat tgcggattat gaatcggcaa tgaataacgc tgtgaaagcg
     9781 gtttcagact ggctgaaaga agaaaaaatg tacaccggtg gctcaattaa agagttgcgt
     9841 gccaaaatca acggttttaa cccaacccaa gctggtgtgg gcgtgcaaaa atcactggag
     9901 catttggtgg aaattttctt aaagccaagc ctcaaagttc accaccctca ttctttggcc
     9961 cacttacatt gcccgacaat ggtaaccagc caaattgcag aagtgttaat taatgccacc
    10021 aaccaatcaa tggactcttg ggatcaaagc ccggcaggtt cgattatgga agagcattta
    10081 attgactggc tacgtcaaaa agtcggctat ggcaaaggaa cttcaggagt ctttacctca
    10141 ggtggtacgc aatcaaacct gatgggcgta ttattggctc gtgactgggc gatagcgaac
    10201 cattggaaaa aggcagacgg ttcagagtgg tcggtgcaag ccgacggtat tcctgccgat
    10261 gcaatgcaaa aagtgaaagt ggtttgttcc gaaaacgctc acttctcggt gcagaaaaat
    10321 atggcaatga tgggaatggg tttccaatcg gtagtgactg taccgtcaaa tgccaatgca
    10381 caaatggacg ttgaagctct tgcgaaaacg ttggcggatt taaaagccga aggcaaaatt
    10441 gtggcttgta tcgtcgcaac cgcagggacg accgatgccg gtgcgattga tgatttaaaa
    10501 gcgatccgta aattagccga tgaacatcag gcttgggttc acgttgatgc tgcttggggt
    10561 ggtgcattat tactgtcgaa agatttccgc cacttcttag acggcattga attaaccgat
    10621 tccattaccc ttgatttcca caagcatttc ttccaatcaa tttcgtgtgg ggcattcttg
    10681 ctaaaagatg aagcgaatta ccgctttatt gattacaagg cagactacct gaattcagaa
    10741 tatgacgaag aacacggcgt gccgaattta gtgtcaaaat cattgcaaac tacccgccgt
    10801 tttgatgcgt taaaattatg gtttaccgtt gaagccttgg gtgaggagct atacgcttca
    10861 atgatcgacc acggggttta tctttctaaa gaagtggaag cctacattaa tgccaccgat
    10921 ggcttggaaa tgttagtgcc ggcacaattt gcctcagtct tattccgtgt cgtaccacaa
    10981 ggttacccgg cagaatttgt ggatacctta aaccaaaatg tggcagatga gttatttgct
    11041 cgtggcgaag ctaacattgg tgtgaccaaa gtcggtgagc atcaatcact gaaaatgact
    11101 actttaagcc cgattgcaac gcttgaaaac gtaagagcat tattagctca ggtgttggca
    11161 gaagcagatc ggattaaaga ttcgattgta aatggaactt atgttccgcc aattgattaa
    11221 actgcgttaa aaataaaaag gtgttttcga catttatttg tgttttaatg tttgcattcg
    11281 gtagtcttta tcgaatgcaa atttttttaa caaggagttt ttatgaaaaa agtcctgggt
    11341 ttaacattga tggcaatggc gttatcggcg tgtaattcaa ctgcttccaa cggttcttca
    11401 caagcaaata caggtgttgc accggcgaat gtgattagcg tgaaacaggc gttagcggca
    11461 gcagatgaca gctacgtgac ggtagaaggc acactcttaa acaaagtcgg tgacgaaact
    11521 tacttattta aagatgcgac agggcaaatc cgtgttgaga ttgacgatga agtatgggca
    11581 ggtcagcacg ttggtagcga aggcaataaa gtccgtttat acggagagat tgacaaaggt
    11641 ttcaataaaa ccgagttaga tgttaaaaca ctcactgttc tgtagaagat caaaccttaa
    11701 tttggttccc cgccttgttt ggcggggttt ttacgttaat taatcaaacc atccttttgc
    11761 aaaaaaacgc aaaattatac cgcttgttcc ttcaatattg tgctacaaga tgctgtgatt
    11821 tgctgcaaaa tagcggtgta atcagtctct tttaaaccaa tattaccgaa atagtgcatt
    11881 tttacatcac gaatcccaca gaactcaaac agcccttttc ccagcgtatg ttcaaatgct
    11941 gctaaaaagc cttttcgttc atatttttca ttggaattac cgagcgttac aaattgttgc
    12001 atttttttgc cggtcagcaa gccgatactt tcaatttccc cgttacgata ggcaaaatca
    12061 taacttaaca ccctgtctaa atagcctttt aaaattgccg gaaagcccat ccaccaaagc
    12121 gggtaaatta acgtaatcac atcagcttca agccaatatt gatgctcaat tgccacatct
    12181 gcttcatagt gtttactgag tgtgccttga aattccgccc agtctaatat cggcttgaag
    12241 ttgagttggt agagatctcg tacaaccacc ttatttggaa cggaagctgc tatcgttttt
    12301 tccaaaatcg catggttaaa gctggaggga ttcgggtgtg cgtaaataat caggtgattc
    12361 atagcgtttt cctttactgt gtcgaggcag ctactagaag attttagcga aactttcttt
    12421 gatgaaaatc aatattttgg cgaatatacc ggcaaatacc ccgtttgagg aatgaacggt
    12481 tttaagtgaa cattagataa tttagcctat tatagtgatg agtaaaaagg tgttttcttg
    12541 actgattatc ctaatcaatc acttcctcgc cgtagctttt tgcgagggca atttttagac
    12601 tcgttaaaaa gccaaaaagt cagtcagcaa gggtatgagc cgattcgtcc gccttgggct
    12661 aatttagcaa attttttaga aaaatgcacc gcttgcggta actgcattca ggcttgcgaa
    12721 acccagatta ttgtaaaagg tgctgcgggc taccctgaaa ttgattttag ccgtggtgaa
    12781 tgcacttttt gtgaaaaatg tgtgcattct tgcgaggcag aggtattccg agcagtttca
    12841 gaacagcctt ggagccataa aattgagatt caagccggct gtttattaaa gctcggaacg
    12901 gaatgccgaa gctgtggcga tagctgtgag atgagagtga ttcgtttccg cccgagttta
    12961 ggtggcattg ccgaaatggt attaaatttg gaaagctgta atggctgtgg ggcttgtttg
    13021 agcgtttgtc cgacaagtgc gataaaaatt cagccattac aaacacaggc aaacagagat
    13081 gagtgaaaaa ttatccgaac aggaaaactg gtatgtgtgc agtctcgtgg tgcaagcaaa
    13141 accggaaaaa ttggaagaag taaaagccga gattctgaag attcctcata ccgaaattca
    13201 cggtgagaaa acagatgaag gcaaattggt cgtgacgatt gaaagcaatg tgcatttagc
    13261 cctttccgat cgaatcgatc aagtaagaga tattaaaggc gtgattgtgg tgtctttaat
    13321 ttctaattac attgatgaac agtgattaat gattattttt gaagccaaac gtgggacata
    13381 gttatggaac tcaatcgtag agattttatg aaagcgaacg ccgctgtggc agctgctgca
    13441 gccgcaggaa ttatgattcc tgtgaaaaac gttcaggcag atgacaccgg tattcgttgg
    13501 gataaagccc cttgtcgtta ctgtggtaca ggttgtagcg tgctagtcgg tactaaagac
    13561 ggtaaagtag tggcaaccca aggtgacccg gatgcagaag taaaccgtgg tttaaactgt
    13621 attaaaggtt atttcttatc caaaatgatg tacggtgcag accgtatgca agagccaatg
    13681 ttgcgtatga aagacggtaa gttcgacaag aacggcgatt tcacgccggt ttcttgggat
    13741 caagccttta gcattatggc ggagaagatt aaaaccacta ttaaaaatga cggcccgaat
    13801 gctgtaggta tgttctcatc aggtcaaaca accatttttg aaggttatgc gaaagtgaaa
    13861 ctttggaaag gtggtttccg ttcaaatacg attgacccga atgcccgcca ctgtatggcg
    13921 tccgctgcgg ttgcctttat gcgtaccttt ggtatggacg agccgatggg ctgctataac
    13981 gacatcgaaa aaaccgatgc gttcgtgctt tggggttcaa atatggcaga aatgcacccg
    14041 attttatggt ctcgtatttc tgatcgccgt ttatcagaca ataaagtgaa agtggtggta
    14101 atgtccactt acgaacaccg ttcgtttgaa ttagccgata caccgattat cttcaaacca
    14161 cactcagatt tagcaatttt aaactacatt gcgaactaca ttatccaaaa cgataaagta
    14221 aattgggatt ttgttaacaa acacaccaaa tttaaacggg gtgaaaccaa tatcggttac
    14281 ggtttacgtc ctgaacacaa attacaacaa aataccaatg cgaaaactgc cggtaaaatg
    14341 tatgacagcg attttgaaga gtttaagaaa atcgttgccc cttatacatt agaagaagct
    14401 caccgtattt caggcgtgcc gaaagatcag cttgaaacat tagcgaaatt atatgcagat
    14461 cctaaccaaa aattagtgtc gttctggacg atgggcttta accaacatac ccgtggtgtg
    14521 tgggtaaacc atatgatgta caacgtccat ttattaaccg gtaagatcgc attagaaggt
    14581 tgtgggccgt tctcactcac gggtcagccg tccgcttgtg gtactgcccg tgaagtgggt
    14641 acatttattc accgcttacc ggcagatatg gtggtcacaa atccgaaaca ccgtgagatt
    14701 gtagagaatt cttggaaatt accggctgga gcaattcctg atgtaccggg ctttcacgct
    14761 acagctcaaa gccgtgcctt aaaagacggc aaaatgaaat tcttatggca gatgtgtacc
    14821 aacaatatgc agggcggccc gaatatcaat gaagagatct tcccgggctg gcgtaatcct
    14881 gaaaccttta ttgtggtttc agatccgtac ccatctgttt cagcagtggc tgctgactta
    14941 attcttccaa cctgtatgtg ggtggaaaaa gaaggggctt acggtaacgc agaacgccgt
    15001 acccaattct ggcgtcagca agttaaaggg ccggcaaatg cccgttcaga tacttggcag
    15061 ttagtagagt tctctaaata cttcaaagta gaagaagtat ggccggaaga gttattagct
    15121 aaagcaccgg aattgcgtgg taaaactcta tacgaaatcc tttaccgcaa cggcaaagta
    15181 gatagctacc aagtgccaac taacattccg ggctatatga atgacgaagc agatcacttc
    15241 ggttactaca ttcaaaaagg cttatttgaa gagtatgcgg aatttggccg tggtcacggt
    15301 cacgatttag ccgactttga cacctaccac aaagtacgtg gcttacgttg gccggtggta
    15361 aaagacgaga aaaccggcga atacaaagaa actttatggc gttaccgtga aggctatgac
    15421 ccatacgtta aagccggtga agaagtggca ttctatggca atgcggacaa aaaagcagtg
    15481 attttaggtg tgccatacga accacctgcg gaagcaccgg acgaagaata tgacttatgg
    15541 ttctgtaccg gtcgtgtact agaacattgg cacaccggta cgatgacccg ccgtgtgccg
    15601 gagttacata aagcattccc aaccaacttg gtttggatgc acccgaatga tgcgaaaaaa
    15661 cgaggcttac gtcacggtga taaagtgaaa gtgatttcac gccgtggtga atatgtatcg
    15721 aacttagata cccgtggacg taataaatgt ccggaaggtt tgatttactc gactttcttc
    15781 gatgccggtc agttaatcaa caaagtgaca cttgatgcga ctgatccaat ttcatttgaa
    15841 accgacttca aaaaatgtgc ggttaaagtg gtgaaagctt aattgtaggg gcaaattatt
    15901 tgcctcagac aagcggtcaa atttgcaaat ttttttacaa aaacgaccgc ttacctaaga
    15961 ggaacccaca atgaaaatgg atccaaatcg tcgccagttt ttaaaaaatg caacccgtac
    16021 cgcagccggt gtttgcggtg taggaatgat tttagggcta cagcaacaac aaagttttgc
    16081 ccgtcaaggg gtagcactac gcccaccggg tgcattgccg gagcaagatt tcctgagtgc
    16141 ttgcacccgt tgcggacaat gtgtgcaggc ttgtccttac gatatgttgc atttggcatc
    16201 gcttatttca ccagtggaag ccggcacgcc atactttatt gcacgggata aaccttgcga
    16261 aatgtgcttg gatattccct gtatgaatgc ttgcccaacc ggggcattaa gcgaagagct
    16321 aaaagacatt gacgatgcac gaatggggct tgcggtgttg ctcgaccacg aaacttgtct
    16381 taactggcaa ggcttgcgtt gtgacgtgtg ttaccgtgtt tgtccgttaa tcgacaaagc
    16441 gattactctt gagaaagtgc ataacgaccg tacctcgatt cacgccaaac tgattccgac
    16501 cgtgcattct gatgcttgca ccggttgcgg taaatgtgaa caggcttgcg tattggaaga
    16561 ggcagcgatc aaagtgttac cgatggattt agccaaaggc ttgctcggtc gtcactatcg
    16621 tttaggctgg caagagaaac aaaatgccgg taaatcgctg attgaagatg ttcacccaga
    16681 gggtttacgc ccagcttatg atgccagaat gccggaaggc ttacacgagc cggtgtatca
    16741 gcatatggaa gtaaaacctg atgtgaaagt ggctacgcca agtcgggcga cacaaaacta
    16801 tgtgccgaac tctacgacgg tggaagctcc ggagcatttc cctgatttag acttgaatct
    16861 caagggggtg aaataatggc agccaattct ccaaaaaatg cgggcttaga ggctcgtcag
    16921 aaactaggtt ggtggcacgc ttaccgcttt ttgattttaa gacgcttaag ccaactcagc
    16981 attattttga tgtttttaag cggcccactt tggaatgtct ggattttaaa aggaaactac
    17041 agcggtagct tattactaga taccgtgccg atgacagacc cattaatgac cgctgaagtg
    17101 ctggcaaccg gctatcgccc tgaatggacg gtattggttg gggcgttaat tatggtggcg
    17161 ttttacggca ttgtcggcag caagctgttt tgtagttggg tctgcccgat gaatatggta
    17221 acagatactg ctgcttggtt aagacgcaaa ctcggtattc gccaaactgc taaaattcca
    17281 cgcaatttac gctatgtgat tttggcggtg attttagtcg gtagtgcgat ttccggaaca
    17341 ctattgtggg agtggatcaa cccggttgcg gctctcggtc gggtgtttgt attcggttta
    17401 ggggcaacgc tttggctggt tttagtggtg ttcttgtttg acttattggt ggcagaacat
    17461 ggctggtgtg gtcatctttg cccgattggt gcaacgtatg ccttaattgg ggcgaaaagc
    17521 ctgattaaag tcaaagtggt agatcgtaac cgttgcgata attgtatgga ttgttacaac
    17581 gtttgtccgg aacctcaagt gttgcgtgtg ccgctacacg gtaaaccgga ggaaagcacg
    17641 gttatcttat ctaaagattg cattacctgc ggacgttgta ttgacgtttg tgccgaaaat
    17701 gtatttgcct tcggaatgcg ttttcaaggc agtattgatg tgaaaaatat ttaattttta
    17761 tctttttgaa aacaagaatt ttaatggagt gaacctcaaa tgagaaaata tctcgccttc
    17821 attttagccg tgatgtccgg ctttgcaatg gcggaagccc ctaagttatc cggatcaatt
    17881 gatgagacaa ccgaaagtgt tgcaccggga tttcataacc cacaaaaaga tgtgggcacg
    17941 attccaacca ctttcccttt ccaaccaccg cttgttccac atagtattcg tggcttacaa
    18001 gtgagcaaaa acgtaaacca ttgtttaggt tgccacgcta ttgaaccatc taaaaccaca
    18061 ggtgcgaccc gtttaccgcc aagccatttt accgaccgtg atggcaaggt gtacgaaaac
    18121 gaatctcctc gtcgttattt ctgtttgcaa tgccacgtgc aacaaactga tgtaaacccg
    18181 attattcaaa ataaatttga atctgtacgt gaatctcaag ctaaataagg agtaagtaat
    18241 gttgagaaga ttttgtaact ggttacgtag cccaagcaaa atggcaattg gtgctgtaat
    18301 ccttatttct gcattaggtg gtattttcgc ttggagtggt tttaatgcag ggttagcaat
    18361 gacaaatact gaagagtttt gttcagattg ccatatgaac gatgttgtgc cggaataccg
    18421 tgcctctgct cactattcaa accgcagcgg tgtgaaagcg atttgttcag attgccacgt
    18481 accgcacgaa ttctttccaa aatggcaacg taaaatcata gccgcaaaag aagtttatgc
    18541 acactttacc ggtaaagtag ataccaaaga gaaatttgaa gctcatcgtg gcgaaatggc
    18601 agaacgtgaa tgggcacgta tgaaagcgaa cagctctcaa gagtgccgta actgtcataa
    18661 ttttgatgat atggacttca ctcaacaaaa aaacattgcg cagcaaatgc acgcattggc
    18721 acaagagcaa ggtaaaacct gtattgactg ccacaaaggg attgcacaca atctcccaca
    18781 tatggaagcg atccagaaaa actttatccc tgatgatttg ctgaaaaaag cagaagagaa
    18841 gaaataactt aacgtgtttc aatagaaaac cctcaatttc cattgagggt ttctttttag
    18901 tagccaagtt agtaaccaat ggcattgaca tttggtaaaa aatcgttgga atttaaccgc
    18961 ttgacgaccg tttctatcgt gccgttttcg tgcaattcaa tttcacgcca gccttgtggt
    19021 tgtaagtcta aggtaaactg gtcgcaattc ggcttaaatt gaatgcaagt agatggggtg
    19081 gcataaatcg gaataccatg ccaaatatca tcaacctgct gatgaatatg accatgcaca
    19141 atcgctttta cattcttaaa ttttgcgaaa atctcggata attcattgct gttttttaag
    19201 ctatgctgat ccagccacgc tgagtttgtc ggcaaaatat tgtggtgcag agcaaccaag
    19261 gtaaaccgtt gcggatattg tgccaatttt tcttctaacc atttaagctg tgagtaagag
    19321 agttcgccat aaggcatacc taccacctgg gtatttagca tcagaagctg ccattgctca
    19381 ccgagtaaaa tttgtttatg gggaaggata tgtttatatt gcgccaaaat ttctgacata
    19441 ctcggctgaa catcgtgatt cccctctagc cacactatcg gcgagtttag cgaattagtt
    19501 atctgtgcaa aatagtgata accagcaata ttgttatctt gaatcaaatc acctgtggca
    19561 agaactagat caaaatattg cttcgcttgt ttgtttattt gctcaataac tgccgaaaaa
    19621 ctttttactg ttttgacatt aagtagagtt tcctccggat tggagaaaag gtgagggtca
    19681 gttatttgta ataacttaat tacccctgtt acgggtaact taatatattc gtgcattcgt
    19741 tttgcatcca ttcaaattct agtaattttt aactggtgag attctatcag taagctaaaa
    19801 gtaaaaatgt ggactatgcc gcatttttga aaaaaagtga gattaatcaa aaaaaattta
    19861 ttattttcga tagctaagac aatggtaagt ttgattaat
//
LOCUS       DANLYI010000024        19526 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.024, whole genome shotgun sequence.
ACCESSION   DANLYI010000024 DANLYI010000000
VERSION     DANLYI010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 19526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19526
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.024"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            101..2710
                     /gene="adhE"
                     /locus_tag="PXD81_000655"
                     /gene_synonym="adhC"
     CDS             101..2710
                     /gene="adhE"
                     /locus_tag="PXD81_000655"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HDL5536710.1"
                     /translation="MAKNTQPFDAQAEVNELVEKGLKALDEFRKLNQEQVDFIVAKAS
                     VAALDKHGILAMHAYEETGRGVFEDKATKNLFACEYVVNNMRHLKTAGVISEDDVTGI
                     TEIADPVGVVCGITPTTNPTSTTIFKALIALKTRNPIVFAFHPSAQQSSAHAAQIVRD
                     AAVAAGAPENCIQWITQPSMEGTSALMKHPGIATILATGGNAMVEAAYSCGKPALGVG
                     AGNVPAYVEKTAKLKQAVYDIVMSKSFDNGMICASEQAAIVDKEIYAEFIKEMQSYGV
                     YLVNKKEKALLEKYIFGIDKAKDENCSGAKLNSAVVGKPAAWIAEQAGFKVPEKTNIL
                     LAECAFVGEAEPLTREKLSPVLALLKSDSTEHGLELSEAMVNFHGLGHSAAIHTENKA
                     LAKTFGERVKAIRVIWNSPSTFGGIGDVYNSFLPSLTLGCGSYGKNSVGNNVSAVNLI
                     NIKRVGRRRNNMQWFKVPSKIYFERDSIQYLKSMHDVHKVMIVTDRSMVDLGFVDRIT
                     EQLRQRRNQVMIQFFTDVEPNPSLQTVQRGTELMRSFQPDTIIALGGGSPMDAAKIMW
                     LFYEQPEVDFRDLVQKFMDIRKRAFKFPQLGRKAKFVGIPTTSGTGSEVTPFAVITDG
                     DIKYPLADYSLTPTIAIVDPALVMSVPSHVAADTGLDVLTHATEAYTSILANDYTDGL
                     ALQAIKLVFENLEKSVKEFDETAREKMHNASTMAGMAFANAFLGICHSMAHKIGGKFH
                     TIHGRTNAILLPHVIRYNGTRPTKVATWPKYTNYVADVRFQDIARMLGLPAETPEQAV
                     EAYAQAVHDLAVRCGVKMSLREQGVDEQAFLNARRELALHAFEDQCTPANPRLAMIED
                     MEAIMTKAYYGKE"
     gene            2857..3753
                     /locus_tag="PXD81_000656"
     CDS             2857..3753
                     /locus_tag="PXD81_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005612495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HDL5536711.1"
                     /translation="MQITILDGGISRELMRRNAPFHQPEWSAAALYEGPHFVQAVHED
                     FIAHGAEVITTDSYAVVPFHIGEKRFAADGKMLADLAGRLAKQAVKNSGNIATQIAGS
                     LPPMFGSYRADLIQMERFAEVAQPLIDGLSPYVDIWLCETQSAIIEPISIKALLPKDN
                     RPLWVSFTLTDDEPTTEPQLRSGESVKQAVEEMVKLGVQAILFNCCQPEVIGEALSVT
                     QKTLAELKATHIRTGAYANAFAPQPKDATANDGLDEVRQDLTLEAYLGWAKKWTEQGA
                     TIIGGCCGIGVEYIETLAKNLK"
     gene            3763..5172
                     /locus_tag="PXD81_000657"
     CDS             3763..5172
                     /locus_tag="PXD81_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011199731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDL5536712.1"
                     /translation="MSNKKIGLISLTALVLSSMIGSGIFSLPQNMAAVAGAEAITIGW
                     LITGVGIIFLGLSFFFISRLRPDLDGGIYTYAREGFGELMGFMSAWGYWLCATIGIVG
                     YLTVAFEGLGVFTDNPNTVIFGQGNTLASFIGSSIIVWLVHALIAGGIKEAAFVNLIA
                     TFVKVAPLILFIGLGIWYFDVDLFNSDVKATALNNSVSDQVKSTMLITLWVFTGVEGA
                     SVLSAHAKKRSDVGLATVLGIIIALALYVAITILSLGILPRETIANMANPSMGPLLDA
                     MMGPTGKVIITACLIVSVLASYISWTMYSAEVPYRGSKNGAFPKILDKVNKNGTPINS
                     LWFTGFIVQFCLVLVFVFEQSYNTLLLISTSMILIPYFLIGAYLFKLAIQTNATWYIK
                     LTGFMAALYGLWIVYAAGLEYLLLSVVLYVPGILLFLYSNYKFNGRLTLSTAEKVILI
                     VLLVIFGYAVIELPALLAA"
     gene            5279..6502
                     /locus_tag="PXD81_000658"
     CDS             5279..6502
                     /locus_tag="PXD81_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF711 family protein"
                     /protein_id="HDL5536713.1"
                     /translation="MQTYQHKALCRVRTITLFLSLTKDKLQWETALNAAKAEFDLLVP
                     AIQTQGYHIQSIRIVTNSFGEYLDLTNIETAKQDLAYLKQLLTQLNDSGLRIRFAIGE
                     AKTPHELALLPELILNYGDLSNACVNIEPDEFGILDNETILQSAQIIKQIAEITPRGE
                     GNFNFTVNFNCKPFIPYFPASYHSSKLPNSFVIGLETPDLLVAVLKELKASPHNDFYR
                     QCYETMSQALQYHVDEVLEAVKSVQHKLQFAFAGIDSSAAPSKNCASMTEVYELMGVP
                     FFGAAGTVEASSLLTKVFKSIKHVPLVGFSGLMLAVTEDLGLAAGTHKAQFDIRALLT
                     YSAVCGIGLDTVPISNEATVEQIAALMRDTGTMAFRLNKPLTVRLFPIPNKSAGEVTE
                     FESDDLCNCRILAVP"
     gene            complement(6540..7736)
                     /locus_tag="PXD81_000659"
     CDS             complement(6540..7736)
                     /locus_tag="PXD81_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDL5536714.1"
                     /translation="MKQVIRFIVFSTLSLFSFSTFAKTIVAIDAGHGGKDPGAIGKNL
                     GILEKSVTLAISKELKALLDADPNFKAVMTRSGDYFIQLPQRTEIARKHRADLLVSIH
                     ADSSPRGDSLKGASVWVLSNSRASDEMGKWLEDHEKQSELLGGAGSVLSSNNERYLNQ
                     TVLDLQFSHSQRAGYELGKSILAKMSNVAALAKSSPQHASLSVLRSPDIPSVLVETGF
                     LSNSTEEAKLASANYRKQVARAIYNGLVAYRAKNAVASSSVSKKSDKEDKQNKKEEKT
                     AKADLTKKPASEKQEKNTQSDKTKLKEKSKKEDKKEDSAKTNKKKEQQLKEKTALEKD
                     QKNQSKKSDSQREIKVSNSGYHIVQQDETVYSIARAYGTTPQKISELNNLKNYQITVG
                     KKLKVK"
     gene            complement(7756..8247)
                     /gene="tsaE"
                     /locus_tag="PXD81_000660"
     CDS             complement(7756..8247)
                     /gene="tsaE"
                     /locus_tag="PXD81_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HDL5536715.1"
                     /translation="MTDSLSFYCEDETKLLEFGQSFALELKKYLLQDQAHSLVVYLNG
                     ELGAGKTTLTRSIVRAFGHQGNVKSPTYTLVEEYQLPPFSLYHFDLYRLADPEELEFM
                     GIRDYFKPQTLCLLEWAVKGKGMIPEADFVIQIDYKNDGRQISLLPQNQTAVDILVNF
                     HQK"
     gene            complement(8247..8801)
                     /gene="orn"
                     /locus_tag="PXD81_000661"
     CDS             complement(8247..8801)
                     /gene="orn"
                     /locus_tag="PXD81_000661"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HDL5536716.1"
                     /translation="MAQNEQNLIWIDLEMTGLDPEKERIIEIATIVTDKDLNILAEGP
                     VLAVHQTDDLLEKMSDWCVKTHTANGLIERVKASKLTERAAELQTLDFLKRWVPKGAS
                     PICGNSIAQDKRFLYKYMPDLADYFHYRHLDVSTLKELARRWKPEILDKFSKGNTHLA
                     LDDIRESINELKFYREHFIQLDQK"
     gene            complement(8811..9290)
                     /gene="coaD"
                     /locus_tag="PXD81_000662"
     CDS             complement(8811..9290)
                     /gene="coaD"
                     /locus_tag="PXD81_000662"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HDL5536717.1"
                     /translation="MSYNVIYAGTFDPITNGHLDIICKASHLFGKVIVAVAQNPSKQP
                     LFSLEERVELVKQSCTQWQNIEVIGFSGLLADFAKQQNAKALIRGIRGADDIDYEIQL
                     AQLNYKLSGDLETIFFPPSIEWRYLSSTMVREIYRHNGDISQFVPQAVKNALKIAKV"
     gene            complement(9292..10572)
                     /gene="waaA"
                     /locus_tag="PXD81_000663"
     CDS             complement(9292..10572)
                     /gene="waaA"
                     /locus_tag="PXD81_000663"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009875356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HDL5536718.1"
                     /translation="MLRLLYICLSYLLQPVVLLLMWYKGRKQPAYRKRLWERYGIYDE
                     SEKPKAKGVVIHAASVGEVIAATLLIKAVSKQYPELPLIVTTVTPTGSGRVQAAFGNS
                     VSHFYLPYDLPDAIERFLNFIDPKLMIVIETELWPNLIRKVNIRKIPFVIANARLSPR
                     SAKRYGWVKGSIKDMLNQISLIMAQDEVSRDRYLALGYAPAKMVNTGNLKFDLEITPQ
                     LHQSVIKTKASLNLTERPIWIAGSTHEGEEKLILDAHKLLLEHYPNLVLILVPRHPER
                     FGLVELLIQKSDLNYVKRTENKTLSQNTQVMLGDTMGEMMLLYGLSDIAFVGGSLVKH
                     GGHNPLEPIAFNIPVVSGLYTYNFPEIFEKLREVKGVIEIESSVEALTESIQLLLKQP
                     QIGQEIAKSGLSVLQENQGALKRHLDLLASYLEK"
     gene            complement(10752..12131)
                     /gene="yegQ"
                     /locus_tag="PXD81_000664"
     CDS             complement(10752..12131)
                     /gene="yegQ"
                     /locus_tag="PXD81_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HDL5536719.1"
                     /translation="MTLKYAKPELLSPAGSLKNMRYAFAYGADAIYAGQPRYSLRVRN
                     NEFNHANLKVAIDEAHALGKKFYVVVNIAPHNSKLKTFIKDLKVVVDMKPDALIMSDP
                     GLIMLVRENFPEMDIHLSVQANAVNWATVKFWRQMGLTRVILSRELSLEEIEEIREQV
                     PDMELEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYKMEEGKIDEVGQI
                     VPKYEPEIEVKNVAPTLGNGEATDKVFLYTESQRPDEQMTAFEDEHGTYFMNSKDLRA
                     VQHVERLTQMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDESLMDTLESL
                     AHRGYTEGFLRRHTHDEYQNYEYGYSISERQQFVGEFTGKRNAEGLAEVAVKNKFLVG
                     DSVEMMTPKGNIVFKIERMLNRKNENVDAALGDGHFVFLDVPADIELDFALLMRNLVN
                     TNTRNPHQA"
     gene            12259..12630
                     /locus_tag="PXD81_000665"
     CDS             12259..12630
                     /locus_tag="PXD81_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5536720.1"
                     /translation="MKLKYLLATTLLAFSTFTAAQSIKTEMMQMNMQANKLMRAESAE
                     EFSEIANDFIIITEKTKGIFPNSVEGDKAAQEDYQAGMQKLIDMVNQASEKAQAGELQ
                     EAKMLISELEMIKREYHKKYK"
     gene            complement(12669..16145)
                     /gene="dnaE"
                     /locus_tag="PXD81_000666"
     CDS             complement(12669..16145)
                     /gene="dnaE"
                     /locus_tag="PXD81_000666"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005604838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HDL5536721.1"
                     /translation="MSDCRFVHLKVHSDFSMINGLAKVKSLVKTAAANNMVAMGLTDF
                     INFCGLVKFYGEALGAGIKPIIGADVYVRTESGNEDYFELTLLAKNNKGYHNITQILS
                     KAYQQGYVEFPMVEREWLAELNEGIIILSGGRMGDVGKALLKENRQEAEEKLAFYQQY
                     YPNHFYLSVCRTGRADEERYIKQAVEFSQKNAIPLVAVNDVVFLKEDDFDAHEIRVAI
                     HDSYTLDDPKRPKKYSPKQYFRTEEEMCKLFADLPSALANTVEIAKRCSVTVRLGEYF
                     LPNFPTGELSTEDFLVKKSKEGLEERLEFLFPDPEERANKRSVYDERLQVELDVINQM
                     GFPGYFLIVMEFIQWSKDNDIPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFLN
                     PERVSMPDFDVDFCMDGRDRVIEHVAETYGRQAVSQIITFGTMAAKAVIRDVGRVLGH
                     PYSFVDRISKLIPPDPGMTLEKAFAAEPKLPELYEADEEVKDLIDMARKLEGVTRNAG
                     KHAGGVVIAPTAITDFSPLYCDSEGLHPVTHFDKNDVEYAGLVKFDFLGLRTLTIIKW
                     ALEMINERLAREGKEPIRIETIPLDDKKSFALLLAAKTTAVFQLESRGMKDLISRLKP
                     DCFEDIIALVALFRPGPLESGMVQNFIDRKHGKEEVSYPDPQYQHECLKPILEPTYGV
                     IVYQEQVMQIAQELAGYTLGGADLLRRAMGKKKPEEMAAQREVFEKGAEAKGIDGKLA
                     GQIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTSEMDNTDKIVG
                     FYDECMNMGLTVVPPDVNSGKHRFSVNEKGEIVYGLGAIKGVGEGPIEAILAARQKEG
                     KFKDLFDLTARVDLKKINRRTFESLIMSGAFDKLGPHRAALMKNLEDALKASDQHAKM
                     EELGQSDMFGVLTETPEEVQNAYANTPKWSEQVILDGERATLGLYLSGHPIGRFLKEL
                     SHYAPNRLNELQPTFRGQMTTVSGIVMASRVAVTKRGSRIGIATIEDRSGKLDITLFS
                     EALENFGHLMQQDQIIVATGSVQFDDFSGGLKMSAREVLSLDEARGRFAKSLALAISK
                     EQLSADFIKKMTAIITPHKEGTLPLHFYYQSPEGRVLLKGGVEWRISPNEAMFSELKA
                     LLGENSVELEFE"
     gene            complement(16370..17467)
                     /gene="lptG"
                     /locus_tag="PXD81_000667"
     CDS             complement(16370..17467)
                     /gene="lptG"
                     /locus_tag="PXD81_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HDL5536722.1"
                     /translation="MQFFSMNILERYIGKTILSAIFLTLFMLIGLGAIIKFVEEFRDV
                     GRGSYDGLKAAYYTFLTIPRDIETFFPIAALLGSLLGLGNLASLSELVVMQASGFSRF
                     RIGLAVMKTALPLVIFTMIIGEWGVPQTEQFARNMRSIAQSGGSMLATKDGFWAKDGN
                     SFIYINHIKNEKSLDNVLIYQFGEGQNKRELQSVLKARTANYSDEKGWVLSKVEKSTV
                     GENQIQQAKQPDQVWQTSLTPSKLGIVSLKPESLSISGLADYVGFLKDTGQDPKRFEI
                     TFWRKVFQPISMAVMMLLAISFIFGPLRSSTMGAKIVIGIVAGFMFYVANIVFGNLSL
                     IATWLPIPVGALIPSLLCLIIVWWLLSKKRD"
     gene            complement(17467..18576)
                     /gene="lptF"
                     /locus_tag="PXD81_000668"
     CDS             complement(17467..18576)
                     /gene="lptF"
                     /locus_tag="PXD81_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HDL5536723.1"
                     /translation="MILSRYLTKEIFKSQLAILFILLLIFFCQQLVRVLSSAVSGKVP
                     ADLVVSLLGLGMPTMAQLMLPLSLFIALLLTLGRFYAESEITVMRACGIGQGLLVKVA
                     MFLSIFTTALAVYNVFWLTPWAINKQSEMLAEAKANPRFAALSAGQFMSAGGYVLFID
                     KIENNSLNDIYVFQPEQQKKNKPSVVVAESGTLQGLPNGDQVLTLAESKRFEGTPQSA
                     DFRISHFENYSAYLGYQDVESDEKLVQRADFKKLMETKTPEAKAELHWRLALILAVPL
                     MALLAVPMSSVNPRQGRFAKLLPALLLYLIYFLLQSSLKSSGASGKLNAEVLMPLVSV
                     AFLVLGVILNAWDSKFMSKLRYQFGAKKLTNKAVA"
     gene            complement(18750..>19526)
                     /locus_tag="PXD81_000669"
     CDS             complement(18750..>19526)
                     /locus_tag="PXD81_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5536724.1"
                     /translation="FVVGLIGLGTQNTIFANDDAQISRYEQAKLRLSYDGNVGNLLQQ
                     LSQRLKVGFIAYDVDITRKISLQNETETAIKTINEQIESQLTNIDVRFEKIGERLFLV
                     VSAKDVAPLLQNEPKEVQFVGDVIFDGEPNLTEQAQSTAEATKVQNILNIATNQEKIM
                     AAKGKKAPQYKTVTKDNIGLKNIRVTALGTFLVFDTSVDAAKLRVKGNFEDIAQGENI
                     IAILHQNTEAPPKIEIEDSQGKKLILEAHKATSSKKSKKQ"
BASE COUNT         5586 a         4434 c         3797 g         5709 t
ORIGIN      
        1 ataaaacatt attaattttt aacgacaaaa taactctttt gggtaataat ttcatcagtt
       61 aaaaaaatta tcaaatttaa attttatgag gaaccaaact atggctaaaa atacgcaacc
      121 atttgatgcc caagcagaag tgaatgagct tgttgaaaaa ggcttaaaag cccttgatga
      181 gtttagaaaa ctcaaccaag aacaagtgga ttttattgtc gctaaagcct ctgttgctgc
      241 ccttgataaa cacggtattt tagcaatgca cgcttacgaa gaaaccggac ggggtgtgtt
      301 tgaagacaaa gccactaaaa acctgttcgc ctgtgagtat gttgtcaata atatgcgaca
      361 tttgaaaacc gcaggggtca ttagtgaaga tgatgtcacc ggtattaccg aaattgccga
      421 ccctgtcggt gttgtctgcg gtatcacccc aaccaccaac ccaacctcaa ccaccatttt
      481 caaagcgtta atcgccctta aaacccgtaa ccctatcgtt tttgctttcc acccgtctgc
      541 tcaacaatct tctgcccacg ctgctcaaat tgtgcgtgat gctgccgtgg ctgccggtgc
      601 accggaaaac tgtattcaat ggattaccca gccttcaatg gaaggtacat ctgctttaat
      661 gaaacacccg ggcattgcaa ctattttagc caccggcggt aatgcaatgg ttgaagccgc
      721 ttactcttgc ggcaaaccgg cattaggagt tggtgcaggc aacgtaccgg cttatgtaga
      781 aaaaaccgct aaattaaaac aagcggtgta tgacattgta atgtcaaaat ctttcgacaa
      841 cggtatgatt tgtgcctctg aacaagcggc gattgtagat aaagaaattt acgctgagtt
      901 tattaaagag atgcaatctt acggcgtcta tttagtgaac aaaaaagaga aagccttatt
      961 ggaaaaatac attttcggta ttgataaagc caaagatgaa aactgctcag gtgcaaaact
     1021 caattcagcg gtggttggca aaccggctgc gtggattgca gaacaagccg gctttaaagt
     1081 accggaaaaa accaatattc tgttagccga atgtgccttt gtgggcgaag ctgagccact
     1141 tacccgtgaa aaactttcgc ctgtactggc tctgttaaaa tccgactcca ccgaacacgg
     1201 tttggaattg tctgaagcga tggtgaattt ccacggttta gggcatagtg ccgccattca
     1261 taccgaaaac aaagccttag ccaaaacctt tggcgaacgg gtaaaagcca ttcgggtgat
     1321 ttggaactca ccatcaacct ttggcggtat cggtgatgta tataactcct tcctgccatc
     1381 gcttacgctg ggctgtggtt cctacggtaa aaactctgta ggtaacaacg ttagtgcggt
     1441 gaatttaatc aatatcaaac gtgtgggcag acggagaaat aatatgcaat ggtttaaagt
     1501 gccatcaaaa atctactttg agcgtgactc aattcaatat ttgaaatcaa tgcacgatgt
     1561 gcataaagta atgatcgtaa ccgaccgctc aatggttgat ctcggctttg tggatagaat
     1621 taccgaacaa cttcgccaac gtcgcaacca agtgatgatt cagttcttta ccgatgttga
     1681 accaaaccca agcctgcaaa ccgtacaacg tggtacagaa ttaatgcgta gcttccagcc
     1741 tgatactatc atcgcacttg gtggtggttc accaatggat gcagccaaaa ttatgtggtt
     1801 attctacgaa caaccggaag tcgatttccg tgatttagtg caaaaattta tggatatccg
     1861 caaacgtgca ttcaaattcc cacaattagg cagaaaagcg aaatttgtcg gcattccaac
     1921 cacctccgga acaggctctg aagtcacacc gtttgcagta attaccgatg gcgatattaa
     1981 atatccgctc gctgactact cgcttacccc aaccattgcg attgtcgatc ctgcgttagt
     2041 aatgtctgtg ccgtctcacg ttgctgccga caccggttta gacgtattaa ctcacgctac
     2101 cgaggcttac acatcgattt tagccaacga ctataccgac ggcttagcct tgcaagccat
     2161 taagttagtg tttgaaaact tggaaaaatc ggtcaaagag tttgatgaaa ccgcccgtga
     2221 aaaaatgcac aatgcttcca caatggcagg gatggcattt gccaatgcct tcttaggtat
     2281 ttgccactca atggcacaca aaatcggggg taaattccac accatccacg gtcgaaccaa
     2341 tgcgatttta ctgccacacg ttattcgcta taacggcaca cgcccaacca aagtggcaac
     2401 ttggccaaaa tacaccaatt atgtcgcaga tgtgcgtttc caagatattg ccagaatgct
     2461 tggcttaccg gcagaaaccc cggaacaagc ggtggaagct tacgcccaag cggtacacga
     2521 tttagccgtt cgttgcggcg tgaaaatgtc gcttcgtgag caaggcgttg atgagcaagc
     2581 cttcttgaat gcccgccgtg aacttgccct acacgcattt gaagaccaat gcacaccagc
     2641 aaacccacgt ttggcaatga ttgaagatat ggaagcgatt atgactaaag cctactatgg
     2701 caaggaataa agcacaattt aatcgttaaa cttactaaat ggccgcttgc aagcggtcat
     2761 ttttcttgaa atttttactt ttcccttcac aaaatacttt cgctctttta caaagcatag
     2821 tatttttatc gctcctttta tcttacagga aatactatgc aaattacgat tttagacggc
     2881 ggaattagcc gtgaattaat gcgtcgcaat gctccatttc atcagcctga atggtctgct
     2941 gccgctttgt atgaaggccc acattttgtg caagcggtac acgaagattt tatcgctcac
     3001 ggggcagaag tgattactac cgacagctat gcggttgtgc cgtttcacat tggcgaaaaa
     3061 cgctttgcgg cagacggcaa aatgcttgcc gatttagccg gtcgccttgc caaacaagcg
     3121 gtcaaaaatt caggaaatat tgcaactcaa attgcaggct cacttccacc aatgtttggc
     3181 tcttaccgtg ccgatttaat tcaaatggaa cgctttgctg aagtcgctca gcctttaatt
     3241 gacgggcttt cgccttatgt ggatatttgg ctttgcgaaa cccaaagtgc gattattgag
     3301 ccgatttcca tcaaagcatt attgccgaaa gacaaccgcc cgctttgggt ctcttttacc
     3361 ctcaccgatg atgaaccaac caccgagcca caacttcgct cgggcgaaag cgttaaacaa
     3421 gcagtcgaag aaatggtgaa gttaggcgtg caagcgattt tatttaactg ctgtcagcct
     3481 gaagtgatcg gcgaggcatt aagtgtaacc caaaaaactt tagccgaatt aaaagctacc
     3541 catattcgca caggagctta tgccaacgcc tttgctccac aaccaaaaga tgccaccgcc
     3601 aatgacggtt tagatgaagt tcgccaagat ttaaccctgg aggcttattt aggctgggca
     3661 aaaaaatgga cggaacaagg ggcaactatt atcggtggct gctgcggcat tggggttgag
     3721 tatattgaaa cgctggcaaa aaatttaaaa taaggaaatc gaatgtcaaa taaaaaaata
     3781 ggattaattt cactcaccgc cttagtgcta agctcaatga ttggctcggg gattttcagc
     3841 ttaccgcaaa atatggcagc cgttgccgga gcagaagcca ttaccattgg ctggctgatt
     3901 accggtgtcg gcattatctt tttaggttta tcgtttttct ttatttcccg cttgcgtccg
     3961 gatttagacg gtggcattta cacctacgca cgggagggtt ttggcgagct gatgggcttt
     4021 atgtcagcgt ggggctattg gctctgtgcc accattggga ttgtaggata cctcaccgtt
     4081 gcttttgaag gtttgggtgt atttaccgac aacccgaaca ccgtgatttt cgggcaaggt
     4141 aacaccctcg cctcttttat tggatcttca atcattgtgt ggctggttca cgccctcatc
     4201 gcaggcggta tcaaagaagc tgcctttgta aacttaattg ccacttttgt gaaagtcgct
     4261 ccgctcattc tgtttatcgg gttaggcatt tggtatttcg atgtggatct gtttaatagc
     4321 gatgttaaag ccaccgcact taataatagt gtaagcgacc aagtgaaaag cacaatgtta
     4381 atcaccttat gggtatttac cggtgtggaa ggggcttctg tgctgtcggc tcacgcgaaa
     4441 aagcgttctg acgtgggact ggcaaccgta ttaggtatta ttattgcact tgctctttat
     4501 gtggcaatta ccattctttc actcggaatt ctgccacggg aaactattgc caatatggca
     4561 aatccgtcaa tggggccgtt gctcgatgca atgatgggcc caaccggcaa agtgattatt
     4621 actgcttgtt taattgtatc ggtgcttgcc tcttacatta gctggacgat gtattcggca
     4681 gaagtgcctt atcgtggctc aaaaaacggg gctttcccga aaattttaga caaagtgaat
     4741 aaaaacggca cgccgattaa ctcactctgg tttaccggct ttatcgtgca attttgcttg
     4801 gtgttggtgt ttgtttttga gcaaagctat aacaccttat tgctgatttc cacctcgatg
     4861 attttaattc cctatttctt aatcggggct tatttattca aattagcgat tcaaaccaac
     4921 gccacttggt acattaaact caccggcttt atggcagccc tttacggatt atggattgtc
     4981 tatgccgcag gactagaata tttgctgctt tcggtggtgc tttatgtacc cggaatttta
     5041 ctcttcctct actccaacta taaattcaac ggcagattaa ccctttcaac cgctgaaaag
     5101 gtcatcttaa tcgtgctact ggtaattttt ggctatgccg tgattgagct gccagcacta
     5161 ttagcagcat aattcccatc aaaaatgacc gcttgcaagc ggttattttt ctaggatctt
     5221 ctgcaaagat tcattagaat agaaacaacg tcaataataa ataaaaagag gagaaacaat
     5281 gcaaacttat caacataaag ccctttgtcg ggtaagaacc attacccttt ttctgagttt
     5341 aaccaaagat aaattgcagt gggaaacggc attaaatgcg gcaaaagcag aatttgatct
     5401 attagttccc gctattcaaa cacaaggcta tcacatacaa tcgattcgaa tcgttaccaa
     5461 cagtttcggg gaatatcttg atctcactaa tattgaaaca gcgaaacagg atttagccta
     5521 tcttaaacag ctattaaccc aacttaacga tagcggttta cgcattcgct ttgccattgg
     5581 tgaggcaaaa acaccccacg aattagcatt actacctgag ctgattttaa attatggtga
     5641 tttatctaac gcttgtgtca atattgaacc cgatgaattt gggattttag acaatgaaac
     5701 cattttacaa tctgctcaaa ttatcaaaca aatcgctgaa attaccccaa gaggtgaagg
     5761 caattttaat tttacggtga attttaactg taaacctttt attccttatt ttccggcaag
     5821 ctaccattca agcaagctcc ctaacagttt tgtgataggc ctagaaacac cagatctatt
     5881 agtagcggtg ttaaaagagc tgaaagccag cccacataac gacttttatc gccaatgcta
     5941 tgaaacgatg tctcaagcct tgcaatatca cgttgatgaa gttctcgaag cagtaaaatc
     6001 ggtacaacac aaattacaat ttgcctttgc tggcattgat agttctgctg ctccgtcaaa
     6061 aaactgtgcc tcaatgacag aagtttatga attaatgggc gtaccatttt ttggggcagc
     6121 cggaacagta gaagcctctt cacttctgac aaaagtcttt aaatctatca aacatgtacc
     6181 gcttgtcggt ttttcaggtt taatgttggc agtaaccgaa gatttaggct tagccgcagg
     6241 cacacacaaa gcccaattcg acattcgtgc tttactaact tatagtgctg tttgtggcat
     6301 tgggttagat accgtaccga tttccaatga agccactgtt gaacaaattg ccgcattaat
     6361 gcgagacacc ggcacaatgg catttagact aaataaaccg cttacagtac gtttatttcc
     6421 aattccgaat aagagtgctg gggaagtgac agaatttgaa agtgatgatt tatgtaactg
     6481 ccgaatttta gctgtacctt aaaataaagg ctgatggata tttccatcag cctttcaatt
     6541 tacttcacct tcaatttttt acctaccgtg atctgataat ttttcaaatt attcagctca
     6601 ctgatttttt gcggcgtagt accgtaagct ctagcaatcg agtaaaccgt ttcatcttgt
     6661 tggacaatat ggtagccact gttagacacc ttaatttctc tttgagagtc tgatttttta
     6721 gactgatttt tttgatcttt ctcaagagct gttttttctt ttagttgctg ctcttttttc
     6781 ttattggttt tagccgaatc ttctttttta tcctcttttt tgctcttttc tttcaatttt
     6841 gtcttatctg attgcgtatt cttctcttgt ttctcagaag caggcttttt agttaaatca
     6901 gctttagcag ttttttcctc tttcttattt tgcttatctt ctttgtccga ttttttactg
     6961 accgaagaac tagcaactgc attttttgcc cgataagcga ctaatccatt ataaatggcc
     7021 cgagcaactt gtttacgata atttgcactg gctagctttg cttcctcggt tgaattagat
     7081 aaaaatcccg tttccaccaa tacagatgga atatcaggtg aacgcagaac acttaagctt
     7141 gcgtgctgag gagacgattt tgctaatgca gccacatttg acattttagc taaaatgctc
     7201 ttgcctaatt catagccggc acgttgagag tgagagaact gtaaatcaag cactgtttga
     7261 tttaaataac gttcattgtt gcttgaaagc accgaaccag ccccgccaag taattccgac
     7321 tgcttctcgt ggtcttccaa ccatttaccc atttcatcgc tagcacggct attagaaagt
     7381 acccaaaccg aagccccttt caaggaatcg ccacgaggcg aagagtctgc gtgaatagaa
     7441 accagcagat cggcacgatg cttacgggca atctcggtac gttgcggtaa ttggataaaa
     7501 taatctcccg agcgtgtcat cactgcttta aaattaggat cggcatctaa caaggctttt
     7561 agctctttag aaattgcgag ggtaacgctt ttttccaaaa tccccagatt tttaccaata
     7621 gcccccggat cttttccccc gtgtccggca tcaatcgcca caatggtttt ggcaaatgtg
     7681 gaaaaactga ataaactcaa tgtgctaaaa acaataaatc tgataacttg cttcattacc
     7741 ttccctacaa gcggtctatt tttgatgaaa atttactaat atatctactg cagtttgatt
     7801 ttgcggcaac aggctaatct gccgtccgtc attcttataa tcaatttgga tcacaaaatc
     7861 agcctctgga atcattcctt tacctttaac tgcccattca agcaaacaaa gcgtttgtgg
     7921 tttgaaataa tcacgaattc ccataaactc cagctcttca ggatcggcta agcgatacaa
     7981 atcaaaatgg taaaggctga aaggcggaag ttggtattct tccaccagag tataagttgg
     8041 gctttttaca ttgccttgat gcccgaaagc ccgtacgatc gagcgggtta aggtggtttt
     8101 acctgcccca agttcgccgt ttaggtaaac aaccagcgaa tgagcttggt cttgcaataa
     8161 atatttctta agttctaacg cgaatgattg accaaattcc agcaatttag tttcatcttc
     8221 gcagtaaaaa gagagagaat ctgtcattat ttttggtcta gctgaataaa atgttcccga
     8281 taaaatttca attcgttaat ggattcacga atatcgtcca acgctaaatg cgtattgcct
     8341 ttactgaatt tatctaaaat ctccggtttc caacggcgtg ccagctcttt taaagtactc
     8401 acatctaagt ggcgataatg gaaataatcg gctaaatcag gcatatattt atacaaaaaa
     8461 cgtttatctt gagcaatgct gttgccgcaa atcggagaag cgccttttgg cacccagcgt
     8521 tttaagaagt ctaaagtttg caactctgct gcccgttcag tcaatttgct cgctttcact
     8581 cgttcaatca gcccgttagc ggtatgggtt ttgacgcacc aatcactcat tttctctaat
     8641 aaatcatcgg tttggtgtac cgcaagtacc ggtccttccg ctaaaatatt taaatcctta
     8701 tcggtcacaa tggtggcgat ttcaataatg cgttcttttt ccggatctaa ccctgtcatt
     8761 tcaagatcga tccaaattaa attttgttcg ttttgagcca ttttatttcc ttacactttt
     8821 gcaattttta gggcattttt gaccgcttgc ggcacaaatt ggctgatgtc accattgtgg
     8881 cgatagattt cccttaccat cgttgaagaa aggtaacgcc attctattga agggggaaaa
     8941 aaaatagttt ctaaatcgcc cgataatttg taatttaact gagcgagttg gatttcatag
     9001 tcaatatcat ctgccccacg aataccacgg attaacgctt tggcattctg ctgtttggca
     9061 aagtctgcta acaatccact aaagccaatc acctcaatat tttgccactg ggtacagctt
     9121 tgtttaacaa gctccactcg ttcttcaaga ctaaaaagcg gctgcttgct tgggttttga
     9181 gcaacggcaa caatcacttt tccaaataag tgagaggctt tgcaaattat gtccaaatgc
     9241 ccattggtaa tgggatcaaa ggtgcctgca taaattacgt tataactcat tttatttctc
     9301 taaataggag gctaataaat ccaaatggcg ttttaatgcc ccttgatttt cctgtaacac
     9361 gcttaagccg gattttgcaa tttcttgtcc gatttggggc tgcttaagca ataattgaat
     9421 gctctcggtc aaagcctcga ctgaactttc aatttcaatc acgcctttca cctcacgcaa
     9481 tttctcaaag atttcaggga aattataggt gtaaagccca gaaaccaccg gaatattaaa
     9541 agcgattggt tcaagtggat tatgcccacc gtgtttgacc aaactgcctc ctacaaaagc
     9601 aatgtcagac aacccataca gtaacatcat ttcccccatt gtatcaccta acattacttg
     9661 agtattttgg cttaacgttt tattctcagt acgttttaca taatttaaat ctgatttttg
     9721 aatcagtagc tcaactaaac caaaacgctc cggatggcga ggcactaaaa tcaacactaa
     9781 attcgggtaa tgctctaata agagtttatg agcatctaaa atcagcttct cttccccttc
     9841 gtgagtactg ccggcaatcc aaatcggtct ctctgttaaa ttcagactag ctttagtttt
     9901 aatcaccgat tgatgaagct gtggcgtaat ttctaaatca aatttcaggt taccggtatt
     9961 aaccattttt gccggtgcat aaccgagtgc taaatagcga tctcggctta cctcatcttg
    10021 agccataatc agactaattt ggttgagcat atccttaatg ctacctttta cccagccata
    10081 acgcttcgca gaacgtggcg ataagcgggc attggcaatc acaaatggaa ttttacgaat
    10141 atttacttta cgaattaaat tcggccaaag ttcggtttca attacaatca tcaatttcgg
    10201 atcgataaaa ttcaaaaacc gctcaatagc atcaggtaag tcataaggca aataaaaatg
    10261 tgaaacacta ttaccaaaag cagcttgcac acgcccagag cctgttggcg ttaccgttgt
    10321 gactatcaac ggaagttcag gatattgctt gctgactgcc ttaatcaaaa gcgttgcagc
    10381 aatcacctca ccaaccgatg ccgcatggat taccacccct tttgctttcg gtttttccga
    10441 ttcgtcataa atcccataac gctcccataa acgtttccga taagccggtt gtttacgccc
    10501 tttgtaccac attaatagca gaaccacagg ctgtaataga taacttaaac aaatatagag
    10561 taaacgtagc atttattttc cttataccat aaccctcacc tcttgtggga gagagtcaaa
    10621 tacgccaata aatcgttcct ctcttgttcg agagggggga acaagcggtt atttttacca
    10681 aaaattttgc aatctatatc ctcaaagaca aaaccacgga ataaccttaa tccgtggttt
    10741 atctcgcatc attacgcttg atgtggattg cgggtattcg tattcactaa gttacgcatt
    10801 aaaagtgcaa aatccaactc aatatctgcc ggcacatcta aaaagacaaa atgcccgtca
    10861 ccgagtgctg cgtccacatt ttcatttttg cggttaagca tacgctcgat tttaaataca
    10921 atgttgcctt taggggtcat catttccact gaatcgccca ctaagaattt gtttttcact
    10981 gccacttccg ccaagccttc tgcattgcgt ttgccggtaa attcgcccac aaattgttgg
    11041 cgttctgaaa tcgaatagcc atattcatag ttttggtatt catcgtgagt atgacgacgt
    11101 aagaagccct ctgtataacc acggtgtgca aggctttcaa gagtatccat taaactttcg
    11161 tcaaacggtt taccggcagc ggcatcatca atcgccttac gatacacctg agccgttctc
    11221 gcacagtaat agaacgattt cgtacgcccc tcaattttca gtgaatgcac ccccatttga
    11281 gttaaacgct caacgtgttg tactgcacgc agatctttag agttcataaa ataggtgccg
    11341 tgttcatctt caaaggcggt catttgctca tcagggcgtt gtgattctgt ataaaggaac
    11401 actttatctg ttgcttcgcc attacctaga gtaggagcca catttttgac ttcaatttcc
    11461 ggctcatact taggcacaat ttgccctacc tcatcaatct taccttcttc cattttatat
    11521 tcccaacggc aagcattggt gcaagtgcct tggtttgggt cacgcttatt gatatagcct
    11581 gaaagcaagc agcgacctga atatgccata cacaaagcac cgtgaacgaa gatctcaagc
    11641 tccatatccg gcacttgttc acgaatttct tcgatctctt cgagtgataa ttcacgggat
    11701 aaaatcacac gggttagccc catttgacgc cagaatttca ccgttgccca gtttaccgca
    11761 ttggcttgca ccgaaagatg aatatccatt tcagggaaat tttcacgcac caacataatt
    11821 aagcccggat cagacataat caaagcgtcc ggcttcatat ccactaccac ttttagatct
    11881 ttgatgaagg tttttaattt tgagttgtgt ggcgcaatgt ttaccaccac atagaatttt
    11941 tttcctaagg cgtgggcttc atcaatcgca acttttaaat tggcgtggtt aaattcatta
    12001 ttacgcacac gcaagctata acggggctgt cctgcataaa tcgcatcggc accataagca
    12061 aaagcgtaac gcatattttt taaagaacct gcaggcgaaa gtagctcagg tttggcatat
    12121 tttaaagtca tattattctc ttttctgata acaagtcagg acttctcgac gtgagaagcg
    12181 taagcggtat attttctaag aaatattgcg tgatggcaag ctaatccatt acaatccgat
    12241 ttttatgtgg ggaacaatat gaaacttaaa taccttttag caacaacatt actggctttt
    12301 tcaacattta ccgccgctca aagcatcaag acggagatga tgcaaatgaa tatgcaagcg
    12361 aataaattga tgcgtgccga atcggcagaa gagtttagcg aaattgcgaa tgatttcatt
    12421 ataattaccg agaaaaccaa agggattttt cccaatagcg tagaaggcga taaagcagct
    12481 caggaagatt accaagccgg tatgcaaaaa ttaattgata tggttaatca agccagtgag
    12541 aaagctcaag caggtgaatt gcaagaagca aaaatgctga tttccgagct tgaaatgatc
    12601 aagcgtgaat atcacaaaaa atacaaataa ttcaacacat taaaagggtg agctaaagct
    12661 caccctttct attcaaattc cagttcaacc gaattctcac caagaagtgc ctttaattcg
    12721 ctaaacattg cctcattcgg gctgattcgc cactctactc cgcctttgag caatacccta
    12781 ccttccgggc tttggtaata aaaatgcagc ggtaaagtgc cttctttatg tggggtgatg
    12841 atagcggtca tttttttaat aaaatctgca gaaagttgct ctttgctaat cgctaacgct
    12901 aaacttttgg caaatcgacc ccttgcttca tcaagggata acacttctcg agccgacatt
    12961 ttcagtccac cgctgaaatc atcaaactgt accgaacctg ttgccacaat aatttggtct
    13021 tgttgcatca aatgcccgaa attttctaag gcttccgaga aaagggtaat gtccaatttc
    13081 cccgagcgat cttcaatagt cgcaatccca atacggcttc ctcgcttggt aactgccact
    13141 cgagatgcca taacaattcc tgaaaccgtg gtcatttgcc ctcggaaagt cggttgcaat
    13201 tcattcagcc gattaggggc atagtgggat aattctttga ggaatctacc aatcgggtgt
    13261 ccgcttaaat acagcccgag cgtggcacgc tctccatcta aaatcacttg ctccgaccat
    13321 tttggcgtat tggcgtaagc attttgcact tcttccggtg tctcggtaag cacgccaaac
    13381 atatcgctct gcccgagttc ctccatttta gcgtgctgat ctgaggcttt aagtgcatct
    13441 tccaaattct tcatcaaggc agctcggtga gggcctaatt tgtcaaaagc accggacata
    13501 atcaggcttt caaaggtgcg gcggttgatt tttttcagat cgacccgagc ggttaaatca
    13561 aatagatctt taaatttccc ctctttttgg cgagctgcca gaattgcctc aatcggtcct
    13621 tccccaacgc ctttaattgc tcctagcccg taaacaattt cgcctttttc atttacgcta
    13681 aaacgatgtt taccgctgtt tacatcaggc ggcacaacgg ttaagcccat attcatacac
    13741 tcatcgtaga acccgacaat tttatcggta ttatccatct ctgaggtcat taccgcagcc
    13801 ataaattctg ccggataatg ggcttttagc caaagtgttt ggtaagaaac caacgcataa
    13861 gctgctgagt gagatttatt aaagccgtag ccggcaaact tttctaccaa gtcaaaaatt
    13921 tgaccggcaa gtttaccgtc aatccctttg gcttccgccc ctttttcaaa cacttcacgc
    13981 tgtgcagcca tttcctccgg tttcttctta cccattgcac ggcggagtaa gtccgctcca
    14041 ccgagtgtat aaccggcaag ctcttgtgca atttgcataa cctgttcttg gtaaacaatg
    14101 accccataag tcggctcgag aataggtttt aagcattcgt gttggtattg cggatcagga
    14161 taagaaacct cttctttgcc gtgtttacgg tcaataaagt tctgcaccat acctgattcc
    14221 agcggccctg ggcggaatag tgccaccaat gcgataatat cttcaaaaca gtccggcttc
    14281 aggcgagaaa ttaagtcttt cattccccgt gattcaagct ggaatactgc tgtagtttta
    14341 gctgccagca acagagcaaa ggatttttta tcatcaagcg gaatggtttc aatccgaatc
    14401 ggctctttgc cttcacgagc caaacgttcg ttaatcatct ccaacgccca tttgatgatg
    14461 gtgagcgtac gcaagcctaa gaagtcaaac ttcaccaatc ctgcatattc cacgtcattt
    14521 ttatcaaaat gggtaaccgg gtgtaagccc tcgctatcgc aataaagcgg cgaaaaatcg
    14581 gtaatcgcag ttggggcgat caccacacca ccagcgtgtt tacctgcatt acgagttaca
    14641 ccttcgagct tacgagccat atcaataagg tctttcacct cttcatcggc ttcatatagc
    14701 tcaggcaatt ttggctcagc ggcaaaggct ttttcaagcg tcattcctgg atcgggcgga
    14761 atgagtttgg agatgcgatc cacaaagcta taggggtgtc caagcactcg ccctacatct
    14821 cgaatgaccg ctttcgccgc catagtaccg aatgtgataa tttgcgatac cgcttgtcgc
    14881 ccgtaggttt ccgcaacgtg ttcaatcaca cggtctcgcc cgtccataca gaaatcaacg
    14941 tcaaaatcgg gcattgaaac acgctcaggg ttgagaaaac gctcaaaaag caaatcaaat
    15001 tccaatgggt caagatcggt aatcttcaac gcataagcca ccaatgagcc tgcccctgag
    15061 ccacgacccg gtcctaccgg aatatcatta tctttggacc attggataaa ctccatcacg
    15121 atcaaaaagt aacccgggaa ccccatttgg ttgatcacat ctaattcaac ttgcaatctt
    15181 tcgtcataaa ccgaccgctt gttcgccctt tcctcaggat cagggaatag aaactctaaa
    15241 cgctcttcta agccttcttt cgattttttt accaagaaat cttcggtgga aagctcaccg
    15301 gtcgggaaat ttggcaggaa atactcgccc aaacgcacgg taaccgaaca gcgtttcgca
    15361 atttcaaccg tgtttgccaa tgcactcggt aaatcggcaa acagcttgca catttcctct
    15421 tcagtacgga aatattgttt cggtgagtat tttttcgggc gtttcgggtc gtctagggtg
    15481 tagctgtcgt gaatggcaac ccgaatttcg tgtgcgtcaa agtcatcttc tttcaagaag
    15541 acaacatcat ttaccgccac taacggaatt gcattttttt gtgaaaattc gaccgcttgt
    15601 ttgatatagc gttcttcgtc tgcccgtccg gtacggcaaa cggaaaggta aaaatgattc
    15661 gggtaatatt gctgataaaa agctaatttt tcttcagctt cttgtcggtt ttctttgagt
    15721 aaggctttac ccacatcgcc cattcgcccg ccggaaagaa tgatgatgcc ctcattcagc
    15781 tctgctaacc attcacgttc caccatcgga aattccacat agccttgctg ataggctttg
    15841 gacaaaattt gggtaatatt atggtagcct ttgttgttct tcgccagtaa tgtcagctca
    15901 aaataatctt cattgccgct ctcggttcta acataaacat ctgccccgat aatcggctta
    15961 atccctgctc ccaatgcttc gccatagaat ttcaccaacc cgcaaaagtt tataaaatcc
    16021 gttaagccca ttgccaccat attattagcg gcagcggttt taactaagga ttttacctta
    16081 gctaaaccat taatcattga gaaatcactg tgaactttta aatgaacgaa acgacaatct
    16141 gacataggaa tataatctaa gtaaagaagt ttcccctctt tagtaaagac tgtcaggttc
    16201 taaaaagtga agccttctgt gaataatccc gttgggcgaa tgttattcgc ccctacaaac
    16261 gagcattatt cttgctcaga gttacattta aaatccgaat gtaggggcga attacattcg
    16321 ccctttcaga acctaacaac cctgctttat ctaaagaagg gagttaaaat taatcacgtt
    16381 ttttgcttaa cagccaccaa acgatgatca aacagagtag gcttggaatt aatgctccaa
    16441 ccggtatagg taaccaagtg gcaattaagc ttaaattgcc aaacacaata tttgccacat
    16501 agaacataaa gccggcaaca ataccaatca caattttcgc ccccatcgta ctactacgaa
    16561 gcggcccgaa aataaaggag atcgcaagta gcatcatcac cgccattgag attggttgga
    16621 atacttttcg ccagaaggtg atctcaaaac gtttcggatc ttgtccggta tcttttaaga
    16681 agccgacata atctgccaag cctgaaatcg agagcgactc cggtttgagt gaaacaatgc
    16741 ctaatttgct aggggttaaa ctggtttgcc aaacttgatc tggctgttta gcttgctgga
    16801 tctgattttc tcccacagtc gatttttcca ctttagatag cacccagcct ttttcatcac
    16861 tgtaattcgc agttcgggct tttaaaacag attgcaattc acgtttgttt tgtccttcgc
    16921 cgaattgata aatcagtacg ttatctaagc ttttttcatt tttaatatga ttaatataga
    16981 taaatgaatt gccatctttc gcccaaaacc cgtcttttgt ggcaagcatt gagccgccac
    17041 tttgggcaat cgaacgcata ttacgggcaa actgctcggt ttgtggcacg ccccattcgc
    17101 cgataatcat cgtaaaaatg actaacggta aggcagtttt catcacggct aaaccaatgc
    17161 ggaaacggga aaaaccggag gcttgcatca ccaccaattc actaaggctc gctaggttgc
    17221 ctaaaccgag taatgagcca agtaatgccg caatcgggaa aaaggtttcg atatctcgtg
    17281 gaatagttaa aaaagtatag tatgccgctt ttaagccatc gtaagatcca cgtcctacat
    17341 cacgaaactc ttccacaaat ttaataatcg ccccaagccc aatcaacata aacagggtta
    17401 aaaaaatcgc ggataaaatc gttttgccga tgtagcgttc taaaatattc attgagaaaa
    17461 attgcattat gccaccgcct tattggttaa tttttttgca ccaaattgat agcgtaactt
    17521 agacataaat ttactatccc acgcatttaa aataacacct aaaactaaaa aggcaactga
    17581 taccaacggc attaacactt ccgcattcaa cttgcctgat gcccctgacg attttagcga
    17641 gctttgtagt aagaaataga ttaaataaag caataacgcc ggcagtagtt tagcaaaacg
    17701 cccttgacga gggtttacac tactcattgg caccgctaat aatgccatta acggtacagc
    17761 taaaatcaaa gcaagccgcc aatgaagctc ggcttttgct tccggcgttt tggtttccat
    17821 taatttcttg aaatcggctc gttgcactaa tttctcatca gattcaacgt cttgatagcc
    17881 taaataagca gaataattct caaaatgtga aatacggaaa tcggcacttt gtggtgtacc
    17941 ttcaaaacgc ttgctctctg ccaaggttaa gacttgatcc ccatttggca aaccttgcaa
    18001 cgtgccggat tcagcaacaa ccactgaagg tttgtttttc ttctgctgtt ccggctggaa
    18061 aacgtagata tcatttagac tgttattttc aattttatca ataaataaca catagccacc
    18121 ggcagacata aattgtccgg ccgaaagagc cgcaaaacgt gggttggctt tcgcttccgc
    18181 taacatttca ctctgtttat taattgccca aggcgtaagc caaaacacgt tataaaccgc
    18241 taatgcagtg gtaaaaatcg agagaaacat cgccacttta accaatagcc cttgcccgat
    18301 accacaagcc cgcattacgg taatttcact ttcggcataa aaccgcccga gggttaataa
    18361 caaggcgata aacagtgaga gtggcagcat taattgtgcc attgtcggca tacctaagcc
    18421 taataagctc accactaaat ctgccggtac tttaccactc acagcagaac ttagaacacg
    18481 cactaattgc tgacaaaaaa agatcagtaa taagataaac aatatcgcca attggctttt
    18541 aaatatctct ttggttaaat atcggcttaa gatcacgttt tttctctata acatttacaa
    18601 atctgaatac cgctaaacac aagcggtcta tttttgttaa ttttttgcaa agagcgcaaa
    18661 ataacttatt cgaatcgcta ataatacctt tttcatacaa aaaaatcacc tatagaaaat
    18721 atcacgcacg gcaaaaccgt gcgtaagtat tattgttttt tcgatttttt cgatgatgtt
    18781 gctttatgag cttcaagaat taatttctta ccttggctat cttcaatttc aatcttaggt
    18841 ggtgcttccg tattttgatg aaggatagct ataatatttt caccttgtgc aatatcttca
    18901 aagtttcctt tcacacgtaa cttagccgca tcaacactag tgtcaaatac taagaatgta
    18961 cctaatgcgg taactctaat atttttaagc cctatattgt ccttcgttac cgttttatat
    19021 tgaggtgctt ttttcccttt tgcagccata atcttctctt gattagtcgc aatattaaga
    19081 atattttgaa ctttagtcgc ttctgctgta gattgtgcct gttctgttaa atttggttct
    19141 ccatcaaaaa taacatcccc aacaaattgc acctctttag gctcattttg caataatggt
    19201 gcaacgtctt tagctgaaac aaccaaaaat aaccgttcac ctattttttc aaaacggaca
    19261 tcaatattgg ttaattgtga ttcaatttgt tcattaattg tcttgatcgc agtttctgtc
    19321 tcattttgaa gcgatatttt acgtgtaata tcaacatcat aagcaataaa accaactttt
    19381 aaacgttgtg ataactgctg aagtaaattg cccacatttc catcataaga aagacgtaac
    19441 tttgcctgct catagcgact aatttgagca tcatcattag cgaaaatagt gttttgagtc
    19501 cctaagccga ttagaccaac aacaaa
//
LOCUS       DANLYI010000025        19272 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.025, whole genome shotgun sequence.
ACCESSION   DANLYI010000025 DANLYI010000000
VERSION     DANLYI010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 19272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19272
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.025"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            complement(76..381)
                     /locus_tag="PXD81_000670"
     CDS             complement(76..381)
                     /locus_tag="PXD81_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HDL5536725.1"
                     /translation="MRKPAHPGQILLDGFIEPNNIKIKELADHLGFSRETLSRVLHGK
                     TPMTANLALSLEEAGISTAKLWLNLQTKYDLWELKQQRLNPVMPFHFGGNYGETAHA"
     gene            complement(390..665)
                     /locus_tag="PXD81_000671"
     CDS             complement(390..665)
                     /locus_tag="PXD81_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011039853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDL5536726.1"
                     /translation="MIKSFKHKGLKQYFEKGITKGIQLNHQRKIDGILDLIDSAESID
                     EFMPFYQCHELKGNRKGIYSMTVNGNWRITFEFVNGDAYILNYEDYH"
     gene            957..1187
                     /locus_tag="PXD81_000672"
     CDS             957..1187
                     /locus_tag="PXD81_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5536727.1"
                     /translation="MNAQATLNNHKDYILCGRKEKRTSDFINVFEVFENEATQEFVIE
                     RAMFRNGKLIDWNQSDKMNAEQAQQLWQAYIH"
     gene            1569..1805
                     /locus_tag="PXD81_000673"
     CDS             1569..1805
                     /locus_tag="PXD81_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5536728.1"
                     /translation="MSNQEFVKLGSVEPSTIDELQKEDIRQAILKSVSITPYYVGLEI
                     AVCQAISAINKFSDGCITEELLDTKNGDKYRKNN"
     gene            2112..2187
                     /locus_tag="PXD81_000674"
     tRNA            2112..2187
                     /locus_tag="PXD81_000674"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2145..2147,aa:Arg,seq:tct)
     gene            2374..2880
                     /locus_tag="PXD81_000675"
     CDS             2374..2880
                     /locus_tag="PXD81_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5536729.1"
                     /translation="MKTKYKIIIERAKQQGLTHIELTQKHCLYLATSVGKLCYGVANN
                     AEQHIKLAAGEIFTLLTIINHQIQFDEDLELHTWKASGTTPPEQRAIRMLHHTSQFAW
                     FAPWLVDSNEKEQNQNTIRINLFNKCSGILIELNGILKEYELTLDECLDEFLKNKHCL
                     NEFSIHKE"
     gene            2884..3243
                     /locus_tag="PXD81_000676"
     CDS             2884..3243
                     /locus_tag="PXD81_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5536730.1"
                     /translation="MTTFSIYTSVWHDSSTKGFRHNIKHKRHDCWRADIRIKQKINDV
                     WVQISRIRKRFKCPEQAKSFVEQWKQIDSPAFMQHQINRQALREELSNLEQRKQKQDG
                     ILEVIEYRISQIKELLK"
     gene            3240..3719
                     /locus_tag="PXD81_000677"
     CDS             3240..3719
                     /locus_tag="PXD81_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5536731.1"
                     /translation="MKRNKVESYTIRLLKNNHTKRYFGEVILNGIKEISTAEMMSEIN
                     AIKAVNKRIEALNKTKAVNLPNYPEIRGHQPIMYNYNFEQIEEDPEQVKPKPSKPKRK
                     PFIPYGLNGYFVDSNGNIRLHLDRRASAETITLETDFFNVLAEMVKRTQEANNAKHA"
     gene            3853..4065
                     /locus_tag="PXD81_000678"
     CDS             3853..4065
                     /locus_tag="PXD81_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5536732.1"
                     /translation="MIGYEDYTDASLQPEEDPSEEAFILAEENIIELLENNGFETIAT
                     ILAGSDGWRNAINAYLAEQNKHRNEF"
     gene            4078..5028
                     /locus_tag="PXD81_000679"
     CDS             4078..5028
                     /locus_tag="PXD81_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5536733.1"
                     /translation="MTTANQQFELILKTESRVVSTNLQTFEEQANQYLATLTTTFETD
                     DDFAKAKEEVKELESIEKKIRDAIKQTQTGEIAELVLTAEQIAERFRQERLTRDKLVK
                     SKETEIKKQIADKAIEEITDTRNKLVKVSDISLALEITMPKHGIANRIAEAQKNKRTI
                     DSLTKAVNAEKALIISEMSVEIGRLTERLEQLAAKSAYLFPDAVKLIATQDDLAPIIQ
                     QRLAEEAEREAAIKAKAEADKVQAEAKAITDKMERKQAVENPQNVAKMNIEEPAQTDG
                     PLGDFIITIRLNQTIQTNAVSIARELKAKFGDSVSLNKAK"
     gene            5069..5863
                     /gene="bet"
                     /locus_tag="PXD81_000680"
     CDS             5069..5863
                     /gene="bet"
                     /locus_tag="PXD81_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage recombination protein Bet"
                     /protein_id="HDL5536734.1"
                     /translation="MTTALQTLTNKLAERFEMGDGSGLVETLKSSAFAGATVSDAQMI
                     ALLVIANQYQLNPWTKEIYAFPGGNGGLTPIVGVDGWVRIINREPQYDGMEFHFTDDY
                     SACTCTIYRKDRSKPIVVTEFMGECKKSSPAWNSHPKRMLRHKAMIQCARLAFGFTGI
                     YDQDEAERIAENEKPPKNITPQNNVVETTAVELISEEQLSQIRQLMQVTGTEEAKILA
                     YIGVQALNQIPKSQAEAVIKKLNLTLDKQNAEKADNGESVGEEIPL"
     gene            5860..6495
                     /locus_tag="PXD81_000681"
     CDS             5860..6495
                     /locus_tag="PXD81_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaJ viral recombinase family protein"
                     /protein_id="HDL5536735.1"
                     /translation="MTGDLIILDCEQGSEEWLNARLGIPTATGIENIVTPSGKKSSAW
                     NSYLAELVAESIEGAKDGFKSSDMARGNELEPLARMAYEFETDTEVVQVGGVYLNAEK
                     QLMVSPDGLIPNLKKGLEIKCPKMKTHIKYLLEGGVPNEYLIQVQSALWVTGYETWDF
                     VSYCPEYQKQPLYIYTAERDPVLMKAFDQYIPQFIKSLYALKGVTAPKGAF"
     gene            6508..6684
                     /locus_tag="PXD81_000682"
     CDS             6508..6684
                     /locus_tag="PXD81_000682"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5536736.1"
                     /translation="MTTQKKYELLQNDTINHNGRTLYRIKAICHFRRTAEYSARATHA
                     TAAECYGGIACTST"
     gene            6705..7157
                     /locus_tag="PXD81_000683"
     CDS             6705..7157
                     /locus_tag="PXD81_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HDL5536737.1"
                     /translation="MKPFDLNKALAGEPVKLRNNDKAFVKYLISDDYIRDNKDHQVQG
                     YTVDEENVFLSEVSWAVSGSHFNDGTIAQYDIVGMWEEPRPAVTLTLPCPLKEPQENM
                     WFIDNDFTIVKSMFANAPFVKKFLPQGRCFASEEDAQEWLDAMRNSRR"
     gene            7235..7762
                     /locus_tag="PXD81_000684"
     CDS             7235..7762
                     /locus_tag="PXD81_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HDL5536738.1"
                     /translation="MNEEKYFSVDVSDETHVIRLHETLEQAKQSCLNGATEAYEFAGD
                     MDDHESYESYEAYDLPYAVYGVVLGRAKSDIRPLTDEERASELFGEAEQVIEPPTLLE
                     NNGWISIEDKLPPIETDVLGLCDISGMQLILIVSRELADNEWYFLSVNQYGLDDDVIA
                     VTHWQPLPEPPKEEK"
     gene            7759..7962
                     /locus_tag="PXD81_000685"
     CDS             7759..7962
                     /locus_tag="PXD81_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5536739.1"
                     /translation="MTDTERLDFIAQRKLDVRYSDLVDEFIIASYTKPHEQYFHWARN
                     ADLRTAIDEAMKEMRGLNNATTN"
     gene            7946..8410
                     /locus_tag="PXD81_000686"
     CDS             7946..8410
                     /locus_tag="PXD81_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HDL5536740.1"
                     /translation="MQQLIKNIEQWAEDRNLINGSTPQKQMLKLMEEFGELCGGIARN
                     NPEMIKDAIGDLIVVCIIKAKQEKQPIYPVQFENHRLNGYRFDISVAVHCLYDIERIK
                     DRGKYSRFFGILHHLALINRFTLNDCLAHAYDQIKDRKGEMQAGIWVKEDDL"
     gene            8504..8674
                     /locus_tag="PXD81_000687"
                     /pseudo
     CDS             8504..8674
                     /locus_tag="PXD81_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253375.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            8910..9401
                     /locus_tag="PXD81_000688"
     CDS             8910..9401
                     /locus_tag="PXD81_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDL5536741.1"
                     /translation="MQTKPILDACCGSRMFHFDKQNPLVLFADNRQIETTMKDRDKIR
                     HLEIKPDVLHDFTNMPYENESFWHIVFDPPHLVQGGENSWLVKKYGKLPCNWREVLKK
                     GFDECMRVLKPNGTLIFKWNETQIPVSEILKVIDCQPIYGHKSGRLNQTHWLAFVKIE
                     QSL"
     gene            9428..9685
                     /locus_tag="PXD81_000689"
     CDS             9428..9685
                     /locus_tag="PXD81_000689"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5536742.1"
                     /translation="MEQIKLSEKAERALILSQLNENSAMIIDLDDVALILNCGYDYVQ
                     SKISKKPGFPEPVQGINGKSVLFKSGDVIRWVNRQARTRKN"
     gene            complement(9682..10671)
                     /locus_tag="PXD81_000690"
     CDS             complement(9682..10671)
                     /locus_tag="PXD81_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDL5536743.1"
                     /translation="MASIRKRGSKYQVEISKKGVRKSATFRTKAEAQAWAYEEERKIE
                     IISKGGVVDILFAEALERYQNEVSINKRTWRREIGRINNILKEPIANRYVQDIKRDDI
                     EDWIEARLKRGLKGSSVERDLSILSNVFTYCLKRWGYISENPMIGVIHPNKVPPRKQR
                     YSQEDIDKILEVSKYREDIKRSNARLAAAMLFAIETAMRAGEISGLTWDNVNFEKRIA
                     HLPMTKNGSSRDVPLSSKAIEILKRLETIKKDNSVFQLPQGNGLSARFFELKEKAGLS
                     YLNFHDTRREALSRLAKKVDVMTLAKISGHKDVKILLNTYYAPNMEEVAHLLD"
     gene            11144..12547
                     /gene="asnS"
                     /locus_tag="PXD81_000691"
     CDS             11144..12547
                     /gene="asnS"
                     /locus_tag="PXD81_000691"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012054399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HDL5536744.1"
                     /translation="MSKFPTVSEILSGKIAVGEEATVRGWVRTRRDSKAGLSFLSVYD
                     GSCFDPIQAIINNDLPNYNAEVLHLTAGCSVVVTGKVVESPAEGQAVELQASKVEVLG
                     WVEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHCLAQAIHRFFHEQGFYW
                     VATPVITASDTEGAGEMFRVSTLDLENLPRGENGKVDFNQDFFGKESFLTVSGQLNAE
                     TYACALSKVYTFGPTFRAENSNTTRHLAEFWMMEPEVAFADLNDNAKLAEDMLKYVFK
                     AVLAERKDDMEFFVKHVDKEAISRLENFINSPFAQVDYTDAIEILLKSGKEFEFPVSW
                     GIDLSSEHERFLAEEHFKSPVVVKNYPKDIKAFYMRLNDDGKTVAAMDVLAPGIGEII
                     GGSQREERLEVLDQRMNEMGLNPEDYWWYRDLRKYGTVPHSGFGLGFERLIVYVTGLQ
                     NIRDVIPFPRAPRNANF"
     gene            complement(12647..13639)
                     /gene="epmB"
                     /locus_tag="PXD81_000692"
     CDS             complement(12647..13639)
                     /gene="epmB"
                     /locus_tag="PXD81_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005604135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HDL5536745.1"
                     /translation="MQPIQIKPLWLTELAQAFNNPIDLLQFLELNPNDFEGDIAARKL
                     FALRVPRMFAEKMEKGNPNDPLFLQAMSLQAEFIEAEGFVVDPLEEQQSPAPNILHKY
                     HNRLLFMLKNSCAINCRYCFRRHFPYEEVKSGKAVWQQGLTYIAEHPELEEVILSGGD
                     PLMAKDQDLDWILTQLEQISHIKTLRIHSRLPVVIPNRITTELCERLSKSRLNVVLVT
                     HINHANEIDAVFANKMAQLKKAGVVLLNQSVMLKGVNDNAQTLKRLSDKLFEYGILPY
                     YLHLFDKVAGASHFYIEDEQAGEIYRELQRITSGYLVPKLAREIAKEPNKILKG"
     gene            complement(13693..14262)
                     /locus_tag="PXD81_000693"
     CDS             complement(13693..14262)
                     /locus_tag="PXD81_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5536746.1"
                     /translation="MKKLITLSAILAVSVATVAEAKRGGGSFRSVSPSKAVATKPVAS
                     QKSTTNQQTQQKDATFANTPNSATAQPQVRQGSTMANVAMGAAAGYILGDMMSSNAHA
                     NQPSQPADANAQAVNSAQNVANGAVPAIAEFKSIGGQIDPFLVEKTDGYRRYCISGVQ
                     YLVAAQGVQTSPTVMVNPNGTPLQCNLIP"
     gene            14429..14995
                     /gene="efp"
                     /locus_tag="PXD81_000694"
     CDS             14429..14995
                     /gene="efp"
                     /locus_tag="PXD81_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HDL5536747.1"
                     /translation="MASYSTNDFKKGLKFIQDGEPCVIVENEFVKPGKGQAFTRTKIR
                     KLISGKVLEINFKSGTSVEAADVVDSNYNYSYSDGDFWYFMHPETFEQISVDEKALGD
                     NVKWLVDNAECIVTLWNGSAIAVTPPNFVELEIVETDPGLKGDTAGTGGKPATLSTGA
                     VVNVPLFVQIGEVIRVDTRSGEYVSRVK"
     gene            complement(15062..15952)
                     /locus_tag="PXD81_000695"
     CDS             complement(15062..15952)
                     /locus_tag="PXD81_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDL5536748.1"
                     /translation="MKENLNDLRAFLLVAQTSSFTKAAAQMGVSQSALSHSIRGIEER
                     LQIKLFHRTTRSISTTEAGERLYQRLLPLFDEIDQEVNGLSEFRNALKGGLRINGNEH
                     VFRDVLADKFAEFVKRYPEVALELVAEDRFVDIVAERFDAGIRLGADVAKDMIALRIS
                     PDVKMTAAASPNYLAQHGTPKTPFDLTEHHCLKHRLATLGGILAWEFRDPITKKIVKF
                     QPQGNLTANNGFLLQHYAKQGLGILWSPQDSIQTELDNGSLVPILTDWAMQYEGYHLY
                     YPNRRQNSPLLKALIDVLRG"
     gene            16059..16634
                     /locus_tag="PXD81_000696"
     CDS             16059..16634
                     /locus_tag="PXD81_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HDL5536749.1"
                     /translation="MKQILILGASGSLANVVIPELLKDNNVQLTLFARRPQSVAKFET
                     ERVKIVQGDVLDFHALTQAMQGVDAVYAGLAGDLKAMAGNVVNAMNEAQVKRLVWISS
                     MGIYGETGEDHGAILEPYRQSAAVIEASELDYTVVRPGWFTNGSEVQYQLTHKGEPFK
                     GHSVSRKSIADFVARILRENSEIRESVGIAA"
     gene            16685..17128
                     /locus_tag="PXD81_000697"
     CDS             16685..17128
                     /locus_tag="PXD81_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HDL5536750.1"
                     /translation="MNLQQIQDRLALKDLVDTFSNLADEKKVAEQMPLFTQDAVVNTY
                     IGGKLVFEMHGVAEIEQVFTTFLTPFHSVYHLNGQHTVPFIDENNATAINYCAVKLVE
                     TKEGKEILHDHSVRYADTYVKQEGKWRIAKRIANFMISESREIRQ"
     gene            17289..18959
                     /gene="glnS"
                     /locus_tag="PXD81_000698"
     CDS             17289..18959
                     /gene="glnS"
                     /locus_tag="PXD81_000698"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HDL5536751.1"
                     /translation="MSEEILTAETGTRANFITHIIDEDLASGKHNNVYTRFPPEPNGY
                     LHIGHAKSICLNFGIAQDYNGLCNLRFDDTNPVKEDVEYVDSIKQDVEWLGFKWEGEP
                     RYASDYFDQLYGYAIELINKGLAYVDELSPDEMREYRGTLTEPGKNSPYRDRSVEENL
                     ALFEKMKNGEFAEGKACLRAKIDMASPFIVMRDPVLYRVKFAHHHQTGDKWCIYPMYD
                     FTHCISDAIERITHSLCTLEFQDNRRLYDWVLDNISIERPLPHQYEFSRLNLEGTLTS
                     KRKLLKLVTDGIVDGWNDPRMPTISGLRRRGYTPASLREFCRRIGVTKQDNVVEYSAL
                     EACIREDLNENAPRAMAVINPVRVVIENFGEKEILKAPNHPNRPELGERDLPFTREIY
                     IDEADFREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDASGRISTIFCTYDPDTLGK
                     NPADGRKVKGVIHWVSAEDNKPAEFRIYDRLFTVPNPGAEEDINAVLNPESLVVKHGF
                     VESSLAHAKPEQDYQFEREGYYCLDSKDGSADNLVFNLTVSLKESVAF"
BASE COUNT         6148 a         3956 c         3932 g         5236 t
ORIGIN      
        1 atattccttg aataaaccgc cctcgtggcg gttttctttt cctaagaaca ataaaatcct
       61 ccgaagaggg cattattaag cgtgagcagt ttctccataa tttccaccaa aatgaaaagg
      121 cataacagga tttaaccgct gctgctttaa ctcccataaa tcatatttag tttgtaagtt
      181 tagccataac ttagccgtac taattcctgc ctcttctaaa cttaacgcta aatttgcagt
      241 cattggtgtt ttgccgtgta atactcttga taaagtttca cgagaaaacc caagatgatc
      301 tgctaattct ttaattttga tgttatttgg ttcaataaat ccatctaata aaatttgccc
      361 cggatgtgct ggtttacgca taactccccc taatgataat cttcataatt caaaatataa
      421 gcatcgccat ttacaaattc aaatgtaatc cgccagttgc cattaaccgt catagaataa
      481 atgccttttc gatttccttt taactcgtgg cattggtaaa aaggcataaa ttcatcaata
      541 ctttctgctg aatcaatcaa atctaaaata ccatcgattt tacgttgatg attaagctga
      601 ataccttttg ttattccttt ttcaaaatat tgttttaagc ctttatgctt gaagctctta
      661 atcataataa cacctagcaa acacaatgtg atacaaatat atcacgcaca ataaaaaaga
      721 caatcttttt taagtgcttt ttctcctaat tgtctttctc ttcttatgtt aagtgtttaa
      781 aaaacaagca atcaaacaac ctttctaaaa ataatttctc tttaaaatca attatttaat
      841 cacgaattga aatttgtttg gctaaaataa ttacaaattg aattgactta atgatttcaa
      901 tttgtgataa taaacccatc aaaacaaaca acgccacaga caaagaggaa accaaaatga
      961 acgcacaagc aacgctaaat aaccacaaag actacatttt atgcggacgc aaagaaaaac
     1021 gcaccagtga tttcatcaat gtgtttgagg tctttgaaaa tgaagccacg caagagtttg
     1081 tgattgaaag agcaatgttc agaaatggca agttaatcga ctggaaccaa agcgacaaaa
     1141 tgaacgccga gcaagctcaa caactttggc aagcctacat tcactaagaa tttttatcaa
     1201 agcccttcac ggagggcttg aataaaggtt ctaaacctcg ccgaaaggca ttgttcttta
     1261 aaaattaaac caaaaacaca tcacgacaag cggattactt agcaatgtaa tgacattgga
     1321 gagacagaac caatctttgc ataaagcggt aaaacgtatg cggtaaatga acttataggc
     1381 aagttgtgaa actctctaag ctactcggac tgaaagaata gtacggtagt tgtggcgaat
     1441 ttactggtga tgtgcgaact agcggaaagg ttaagcactg gcagtgaatc gggaagcaca
     1501 aacgctggtt caattccaaa gcatatttga gaaagtgtgc tttggaatat taataacagg
     1561 agaacaaaat gagtaatcag gaatttgtta aattaggcag cgttgaacct tcaacgattg
     1621 atgaattaca gaaagaagat attaggcagg caatattaaa atccgtctct ataacgccat
     1681 attatgtggg gctggaaata gcggtttgcc aagcaatatc agccatcaat aaatttagtg
     1741 atggctgtat tacagaagag cttttagata cgaaaaatgg tgataaatac cgaaaaaata
     1801 actaatcaat aacaccaaag tgatttcatt ttttcggcat tagccctaga tgttgcccaa
     1861 gtattatatt cctcgaagtt gtggcaaaca agaggacttg gcccggtggc tcacaagtat
     1921 aaaaaagagc caccatctct aacagctctc aacagagagt gagacaaata accccgtttt
     1981 agttttaatg ttttctaaag tcggcgttag gcagcgaaac gaatgtacta gagagttgtt
     2041 agagatggtt agtccatcac attccttaga ttgaagtaag ccctgccgtt ggggttaagc
     2101 aacggtactt tgaccgcata gttcagtgga tagagcagct gccttctaag cagtgggtcg
     2161 agagttcgaa tctctctgcg gtcgccatcc aaaaccgctt tacgcacatc atcgatgacg
     2221 agcctacgaa gtggaaacct accgtgtaga gcggttttga atggctaagt tgtgtaactg
     2281 ctacaactta aaaagagcgg ttgataataa acataatgac tccgtttccc cgcctcgtgc
     2341 ggggcttttt ttatccacat aaaaggattc aaaatgaaaa ctaaatacaa aattattatt
     2401 gagcgagcaa aacaacaggg tttgacccat attgagctta ctcaaaaaca ttgtctgtat
     2461 ctcgctacat cagtagggaa actgtgctac ggggtagcta ataatgcaga acaacatatt
     2521 aaacttgctg ctggagagat ttttacacta ctaacgatca ttaaccatca aatccagttc
     2581 gatgaagact tagagcttca cacgtggaaa gctagtggaa caaccccacc cgagcaaaga
     2641 gcgatacgta tgttacatca tacctcgcaa tttgcttggt ttgctccttg gttagtggac
     2701 agcaatgaaa aagaacaaaa ccaaaataca atcaggatta atttatttaa caaatgctct
     2761 gggattctga ttgaactaaa tggcattctt aaagaatacg agctaactct tgatgagtgc
     2821 ttagatgaat tcttgaaaaa taagcattgt ttgaatgaat ttagtattca caaggagtaa
     2881 aaaatgacga cgtttagtat ttataccagt gtatggcacg acagctccac gaaaggcttt
     2941 cgccacaaca ttaagcataa acgccacgat tgctggagag cggatatccg cataaagcaa
     3001 aaaatcaatg atgtgtgggt gcagatttca aggatacgca aacgcttcaa gtgccctgaa
     3061 caggcaaaat cctttgtaga acaatggaaa caaattgatt cacctgcatt tatgcaacac
     3121 caaatcaacc gccaagcatt aagagaagaa ttatcaaacc ttgagcaacg caaacagaag
     3181 caagacggaa tacttgaggt gattgagtat cgtattagcc aaatcaagga gttgctgaaa
     3241 tgaaacgcaa taaagtagaa agctatacta tccgcttatt aaaaaataat catacaaaac
     3301 gctacttcgg agaagttata ttaaacggca taaaagaaat cagcacggca gagatgatgt
     3361 ctgaaattaa cgctattaag gcagtaaata agcgcattga agctctgaat aaaaccaaag
     3421 ccgttaattt gcctaattat cctgaaatca ggggtcatca gcctattatg tacaattaca
     3481 attttgaaca aattgaggaa gatcctgaac aggttaagcc taaaccatca aaaccaaaac
     3541 gcaagccatt cataccttat gggctaaacg gctactttgt ggatagcaac ggtaatattc
     3601 gcttacatct agacagacga gcaagtgccg aaacaattac ccttgaaact gatttcttca
     3661 acgttcttgc cgaaatggta aaacgtacac aagaggcaaa caatgctaaa catgcttaga
     3721 caatggatta aatacggctt atacgccctg ctttttatgg ttgccatcac cctcctaatg
     3781 caatgaagag cttatccctg aaagcacctg caagcctgaa acttgcgaat ttaaagtaac
     3841 ggagaaagtg tgatgatagg atacgaagac tacacagatg cttcacttca accagaagaa
     3901 gaccctagcg aagaggcttt catcttggct gaagaaaaca tcatcgagct attagaaaac
     3961 aacggctttg aaaccattgc caccatccta gccggttctg atggctggcg taacgcaatc
     4021 aacgcctacc tagccgaaca gaacaaacac agaaatgaat tttagaggaa taagaaaatg
     4081 acaactgcaa atcaacaatt tgaacttatt ttaaaaaccg aaagccgagt ggtttctacc
     4141 aacttgcaaa cttttgaaga gcaagcaaat caataccttg ccacgctcac caccaccttt
     4201 gaaaccgatg atgattttgc aaaagcaaaa gaagaagtga aggagcttga aagcattgag
     4261 aaaaaaattc gtgacgcaat taaacaaacg caaactggcg agattgcgga attggtatta
     4321 acagcagaac aaatcgctga acgtttccgc caagagcgat taacccgaga caagctcgtt
     4381 aagagcaaag aaacggaaat caaaaaacag attgccgata aagccattga agaaatcact
     4441 gataccagaa acaagctggt taaagtaagt gatatttccc ttgccttaga aatcacaatg
     4501 ccaaaacacg gcattgctaa tcgtattgcc gaggcacaga aaaataaacg cacgattgac
     4561 agcttaacca aagccgtgaa cgccgaaaaa gcgctgatta tcagcgaaat gtcggtagaa
     4621 atcgggcgct tgactgagcg attagaacag cttgctgcaa aatcagctta tctttttcct
     4681 gatgcagtga aattgatcgc aacccaagac gacctcgccc caattattca acaacgcctt
     4741 gccgaagaag ccgagcgtga ggcggcgatt aaagccaaag cagaagccga caaagtacaa
     4801 gccgaagcca aagcgattac tgataaaatg gagcgcaaac aagcggtcga aaatccgcaa
     4861 aatgtggcaa aaatgaacat cgaagaaccg gcacaaaccg atgggccatt aggcgatttt
     4921 atcattacta ttcgcttaaa ccaaacaatc caaaccaacg cagtcagtat tgctcgtgag
     4981 ctgaaagcga aatttgggga tagcgtttcg ctcaataagg ctaaatgatt tgataccgca
     5041 caaattcaac ctaaataagg atcatattat gacaactgca ttacaaacac taaccaacaa
     5101 acttgccgag cgttttgaaa tgggagatgg ctcaggatta gttgagacac ttaaatcgtc
     5161 tgctttcgca ggggcaaccg taagcgatgc tcaaatgatt gcactattgg ttattgctaa
     5221 ccaataccaa ttaaaccctt ggacaaaaga aatttatgca ttccccggtg ggaacggtgg
     5281 attaacgcct attgtcggcg ttgatggttg ggttcgcatt attaaccgtg agcctcaata
     5341 tgatggaatg gaatttcatt ttaccgatga ctattccgcc tgtacttgta ctatctaccg
     5401 taaagataga agtaaaccga ttgtggtgac ggaatttatg ggggaatgta aaaagagctc
     5461 tccagcttgg aactctcacc caaaaagaat gttacgccat aaagcaatga tccaatgtgc
     5521 aagattagcc tttggcttta cgggtattta cgaccaagac gaagcagagc gtattgcgga
     5581 aaatgaaaaa ccacctaaaa acatcacacc acaaaataac gtggtagaaa ccactgccgt
     5641 tgaactcatt tcggaagaac aactgtcgca aatccggcag ttaatgcaag taaccggtac
     5701 ggaggaggcg aaaattctcg cctatattgg cgttcaagca ctgaaccaaa tccctaaatc
     5761 gcaggcagag gcagtgatta aaaagctcaa tcttacgcta gacaagcaaa acgctgaaaa
     5821 ggcggataat ggcgaaagtg tcggtgagga aatcccacta tgacaggcga tttaatcatt
     5881 ttagattgcg aacaaggatc agaagaatgg ctcaatgcca gattgggcat tcccaccgcc
     5941 acaggcattg aaaatatcgt tactccaagc ggtaaaaaat cgtccgcttg gaatagctat
     6001 cttgccgaac tggttgccga aagcattgag ggagcaaaag acggttttaa gtcgtctgat
     6061 atggcacgag gcaacgagct tgaaccgctt gcacgaatgg cttacgagtt tgaaaccgac
     6121 accgaagtcg tgcaagtcgg cggtgtgtac cttaacgccg aaaaacagtt aatggtaagt
     6181 cctgacggct taattcccaa tctaaaaaaa gggttggaaa tcaaatgccc gaaaatgaaa
     6241 acccacatca aatacctgct agaaggcggt gtgccgaatg aatatcttat tcaagtgcaa
     6301 tctgcccttt gggtaacagg ctacgaaacg tgggactttg tcagctactg ccccgaatac
     6361 caaaaacaac cgctttacat ctacacagcc gaacgtgatc cggtattgat gaaagccttt
     6421 gatcaatata tcccgcaatt tatcaaatcc ctttatgcat tgaagggggt aaccgccccg
     6481 aaaggggctt tttaataggt agccaatatg acaacccaaa agaaatacga actactccaa
     6541 aacgacacaa tcaaccacaa cggcagaaca ctctaccgca tcaaagccat atgccacttt
     6601 aggcggacgg ctgaatattc cgcaagagcc actcacgcca cagccgctga atgctatggt
     6661 ggaattgcct gtacatcaac ataaccacaa acacggagaa taaaatgaaa ccatttgatt
     6721 taaataaagc attggcaggt gagccagtaa aactaagaaa taatgataag gcatttgtaa
     6781 aatacctaat tagtgatgac tatattagag ataataaaga ccaccaagta caagggtata
     6841 cagttgatga agaaaatgta tttttgtcag aagtatcgtg ggctgttagc ggatctcatt
     6901 tcaatgatgg aacaatagct caatatgata ttgttggtat gtgggaagaa ccaagaccag
     6961 cagtaacatt gacgttacct tgcccattaa aagaacctca agaaaatatg tggtttattg
     7021 acaatgattt taccatagta aaatctatgt tcgcaaatgc cccctttgta aaaaaattct
     7081 tgccacaagg gcgatgtttc gcttcagaag aagacgctca agaatggtta gatgcaatgc
     7141 gaaatagtcg taggtaaatc ggcagcacta acagctgaat gctgctgttg aatttcttgt
     7201 atatccacat aaacacggaa cgcggagaaa gaaaatgaat gaagaaaaat atttttcggt
     7261 cgatgtctcg gatgagacac atgtaataag gttgcacgaa acattagagc aagcaaaaca
     7321 aagctgttta aatggtgcta ctgaagccta tgagtttgcg ggtgacatgg atgatcacga
     7381 aagctatgaa agctatgaag cctatgattt accctatgca gtttatggtg ttgttttagg
     7441 tagagcaaaa tcagatattc gtccactaac agacgaagaa agagcatcgg agttatttgg
     7501 ggaagctgaa caagttattg aaccaccaac acttttagaa aacaacggct ggatttccat
     7561 tgaggacaaa ttaccaccta ttgaaacgga tgtgctaggg ttatgcgata tttcaggaat
     7621 gcagttaatt ttaatagttt caagagaact tgccgataac gagtggtatt ttcttagtgt
     7681 aaatcaatat gggcttgatg acgatgtgat tgcagtaacc cactggcaac cacttcccga
     7741 accaccaaag gaggaaaaat gaccgacacc gaacgcttag actttatcgc ccaacgcaaa
     7801 ttagatgtcc gatattctga tttggtggac gaatttatta ttgctagcta taccaaaccg
     7861 cacgagcagt attttcattg ggcaagaaat gccgatttac gcacggcgat tgatgaagca
     7921 atgaaagaaa tgagaggact aaacaatgca acaactaatt aaaaacatcg aacaatgggc
     7981 ggaagaccgc aatttaatta acggctcaac cccacaaaag caaatgctca aactgatgga
     8041 agagtttggc gaactctgcg gaggcattgc ccgcaataat cctgaaatga ttaaagatgc
     8101 gattggggat ttgattgttg tctgtattat taaagcaaaa caagaaaaac aacctattta
     8161 ccctgtacaa tttgaaaatc atagattaaa tggttacaga tttgatattt cggtcgctgt
     8221 tcattgtcta tatgatattg aacgcattaa agacagaggt aaatatagcc gtttttttgg
     8281 catattacac catttagcct taataaatag atttacgctt aatgactgcc tcgctcacgc
     8341 ctacgaccaa atcaaagatc gcaagggcga aatgcaagct gggatttggg ttaaagaaga
     8401 cgatttatag tacctaagct ataaaaaccg aaacatcttg cagaaaagca ttccgccact
     8461 ctataagagt ttcggtttct tcttcgttgg cgttgattat gtttacacgc acgctaccga
     8521 gtatcaaacc acgcttggcg taatgaagcg gatacttgaa ccgatgttag ccgacttcga
     8581 ggtcgatccg tttgaagagg ctcactacca caaagcccta actaccttaa ggcaatacca
     8641 gccgaaaggc ttaggcggct tagtccgaat ttaatttgca aaaaaatcac acaatccgac
     8701 cgcttgtgaa acaacaagcg gcggatttcc atacccaaat tttgaggaca agccgatgaa
     8761 atacgcaaaa atcattctat ttttaaccgc ctttgcagtt gctgctgacc atctagagtt
     8821 acgcaacgac tgcgatggca aaatttgtat ggctcaacgc taattaaccc aaccgcttgt
     8881 tccgcaagcg gttatttttt ggaggaaaaa tgcaaacaaa accgatatta gatgcgtgtt
     8941 gtggttcccg tatgtttcat tttgacaagc aaaatccact cgtcctgttt gctgataatc
     9001 gacaaataga aaccacaatg aaagaccgag acaaaatccg ccatttagaa atcaaacctg
     9061 atgttttaca cgatttcacg aatatgccat acgaaaacga gagtttttgg cacattgttt
     9121 ttgatccgcc acatttagtt caaggtggag aaaatagctg gcttgtaaag aaatacggca
     9181 aattaccttg caactggcga gaagtcttga agaaaggctt tgacgaatgt atgcgagtgc
     9241 taaaaccaaa cggcacgctt attttcaagt ggaatgaaac acaaatcccc gtttcagaaa
     9301 ttttaaaagt tattgattgt cagccgattt atggacacaa aagcggacgc ttaaaccaaa
     9361 cacattggct ggcgtttgtg aaaattgagc aatccctcta aatgaggggt ttatttttgg
     9421 agagaatatg gaacaaatta aactttcaga aaaagcggag agagccttaa ttctttctca
     9481 gctcaacgaa aattcagcaa tgattattga tttagatgat gttgccctga ttttgaattg
     9541 cggatatgac tatgtgcaat ccaagatcag taaaaagccc ggtttcccag agccagtgca
     9601 aggcattaac gggaaatcag ttcttttcaa aagtggcgat gttatccgtt gggtcaaccg
     9661 tcaagcacga acacgcaaaa attaatccag caaatgagcg acttcttcca tatttggggc
     9721 gtagtaagtg ttaagcaaaa tcttcacatc tttatgccct gaaattttgg ctaaggtcat
     9781 cacatcgact ttttttgcca agcgtgaaag agcctctctc ctcgtgtcgt gaaaattcaa
     9841 ataagaaagc ccagcctttt cttttaactc aaagaatcta gcacttaaac cattcccttg
     9901 tggaagctga aaaacggaat tatccttttt gatggtttcc agtcttttca agatctcgat
     9961 tgcctttgat gataacggaa cgtctcttga tgaaccgttt tttgtcatag gcaaatgagc
    10021 tattcttttc tcaaaattca cattatccca cgttaaaccc gaaatctcac cggcacgcat
    10081 tgccgtttca atcgcaaata acatagccgc agccaatcga gcattagagc gtttaatatc
    10141 ttctcggtat ttgctgactt ctaagatttt atcaatatct tcctgggaat aacgctgttt
    10201 tcttggtggc actttgtttg ggtgaataac accgatcatc ggattttcac taatatagcc
    10261 ccagcgtttt aagcagtagg taaatacatt gcttaaaata gacaaatccc tctcaacact
    10321 tgagcctttt aaccctctct tcaatctagc ttcgatccaa tcttcaatat catctcgctt
    10381 aatatcttgt acataacgat tcgcaatagg ctctttcaat atgttattga ttcgacctat
    10441 ctctcgtcgc cacgtcctct tattaatcga tacttcattt tgataccgct ctaacgcttc
    10501 cgcaaataaa atatcaacta ccccaccttt cgagatgatc tctattttac gctcttcttc
    10561 gtaagcccat gcctgtgcct ctgcttttgt gcggaaggta gctgattttc tcaccccttt
    10621 cttcgagatt tcgacttgat atttactgcc ccgcttgcga atgctcgcca tatttttacc
    10681 tgccttttgt cgtaaatttg tcgtaattta taccatacaa taaccgacaa taacagataa
    10741 taaccgattt tatgaaatca tcttaaggca aagaagagag gaaaaactta agataaccta
    10801 ttgaaataac tataataaac gataataacc gacaaaaaac ggaaataaaa aaaagcccct
    10861 aatcaaaaaa gcgaaaaggg gcggaggtct tacctaagga aatatgaaaa aaacttgcat
    10921 aaatctgtta catatactca gactgcctaa ctgaacgaaa gttcattaaa aaataaaaaa
    10981 ttttttaaca ccaagcaaaa tcagatttaa ctacttgcaa tgcttatata gactcacaaa
    11041 tttgcaaata attccgtaaa attattatat ttccccctat tttcttgtag aatacggaac
    11101 gtttcttata ttaaaactca caacaaagaa tttgaggtat tcaatgtcta aatttccaac
    11161 agtttcggaa atcctatccg gcaagatagc ggtaggtgaa gaagcgacag tgcgtggttg
    11221 ggtgcgtacc cgccgtgatt caaaagccgg tttatctttc ttgagcgttt acgatggttc
    11281 ttgctttgac ccaattcaag cgattattaa taacgatcta ccaaattata atgccgaagt
    11341 attacattta accgcaggtt gctctgtggt ggtgaccggt aaagttgtgg aatcccctgc
    11401 tgaaggtcaa gccgttgaat tacaagcaag taaagtggaa gtgcttggtt gggtggaaga
    11461 tcctgatacc tacccaatgg cagcgaaacg ccactctatc gaatatctgc gtgaagtcgc
    11521 tcacttacgc cctcgtacga acttaatcgg tgcagttgct cgtgttcgtc actgtttagc
    11581 tcaagctatc caccgtttct tccacgagca aggtttctat tgggtagcaa cacctgtaat
    11641 taccgcctca gatactgaag gtgcaggtga aatgttccgt gtttctacgc tggatttaga
    11701 aaatctacct cgtggcgaaa acggcaaagt ggattttaac caagatttct tcggtaaaga
    11761 atcgttctta accgtatccg gccagttaaa tgcggaaacc tatgcgtgtg ccttaagtaa
    11821 agtttatact ttcggtccga cattccgtgc agaaaactca aacacaactc gccatcttgc
    11881 agaattctgg atgatggagc cggaagttgc ctttgccgac ttaaacgaca atgcaaaatt
    11941 agccgaagat atgctgaaat atgtgtttaa agcggtgctt gcagaacgta aagatgatat
    12001 ggaatttttc gttaaacacg ttgataaaga agcgattagt cgtttagaaa actttatcaa
    12061 ttcgccattt gctcaagtag attacaccga tgcgattgag atcttattga aatccggcaa
    12121 agaatttgaa ttcccggtgt cttggggaat cgatctttcc tctgaacacg agcgtttctt
    12181 agccgaagaa cactttaaat cgccggtggt cgtgaaaaac tatccaaaag acattaaagc
    12241 cttctatatg cgtttaaatg atgacggcaa aacggttgcc gcaatggacg ttttagctcc
    12301 gggaatcggt gaaattatcg gtggttctca acgtgaagaa cgtttagaag tgttagatca
    12361 gcgtatgaat gaaatgggct taaaccctga agattactgg tggtatcgtg atttgcgtaa
    12421 atacggtaca gtgccacact caggtttcgg tttaggtttc gagcgtttaa tcgtgtatgt
    12481 aaccgggtta caaaacattc gtgatgtaat cccattccca cgtgctccac gcaatgcgaa
    12541 cttctaattt gcaaaaaagt aagagaattt aaccgcttgc aacaagcggt taaaaaaggc
    12601 gaaaatttta ccattttcgc cttttgtttc ttgatttctg tttaagctaa cctttcaaaa
    12661 ttttatttgg ctctttcgct atttctcttg ccagctttgg cacaagatag cctgaagtga
    12721 tacgctgtaa ttctcggtaa atctcaccag cctgttcatc ttcaatatag aaatggctcg
    12781 cccctgccac tttatcaaat aaatgcaaat agtaaggcaa aatcccatat tcaaaaagtt
    12841 tatcacttaa cctttttaag gtttgagcat tatcgtttac accttttaac atcaccgatt
    12901 gattcagcaa tacaacgccg gcttttttca gttgagccat tttgtttgca aaaacagcat
    12961 caatttcatt ggcgtgatta atatgggtaa ccaacacgac attcaagcga gattttgata
    13021 accgttcaca taattctgtc gtaattcgat tgggaataac gaccggcaag cgtgaatgaa
    13081 tacgcagggt tttgatatgc gaaatctgct ctaattgcgt taatatccaa tctaagtcct
    13141 gatcttttgc catcagcgga tcaccaccgg acagaatcac ttcctccagc tcgggatgct
    13201 cagcaatata agttagtccc tgttgccaca cagctttacc gcttttcact tcctcataag
    13261 ggaaatgacg gcgaaaacag taacggcagt taatcgcaca actgtttttt agcataaata
    13321 acaaacggtt atggtattta tgtaaaatat tcggtgctgg gctttgttgc tcttccaatg
    13381 gatctaccac aaacccttct gcctcaataa attccgcctg taaagacatt gcttgcaaaa
    13441 aaagcggatc gtttgggtta cccttttcca ttttttcggc gaacattctt ggcactcgaa
    13501 gagcaaataa tttacgggca gctatatcgc cctcaaaatc atttggattg agttcaagaa
    13561 attgtaataa gtcgataggg ttattaaagg cttgagccag ctcggttagc caaagcggtt
    13621 tgatttgaat tggttgcatt gaatattgta gatacaaggg gcaggttttt gcctgcccgc
    13681 aaataaaaat aattatggaa ttaagttaca ttgtaatggc gtgccatttg gattaaccat
    13741 tacagttggt gaggtttgta caccttgtgc agcgactagg tattgcacgc ctgaaataca
    13801 ataacggcga taaccatcgg ttttttctac taaaaacgga tcgatttgac cgccaatact
    13861 cttaaattct gcaatagcag gaactgctcc atttgcaaca ttttgtgcag aattgaccgc
    13921 ttgtgcattt gcatccgcag gctggctagg ctgatttgca tgtgcatttg atgacatcat
    13981 atcacctaaa atataaccgg ctgctgcccc catcgctaca tttgccatcg tgctgccttg
    14041 acgcacttgt ggctgggcag ttgcactgtt tggcgtattg gcaaaagtag catctttttg
    14101 ttgcgtttgc tgatttgtgg ttgatttttg agaagctacc ggcttagttg ctaccgcttt
    14161 tgacgggcta actgaacgga agctcccacc gccacgtttt gcctctgcca cggttgcaac
    14221 agaaacggct aaaatcgcag aaagagtaat cagttttttc attcgcattc cttatcaata
    14281 aagtttgaaa aattatagca aaaatcgcaa gagatctttc gctaattttg tgaatttagg
    14341 ttattatatg gctctttttg acaaacccaa tagggttggg ttcaaaattt gttaatttat
    14401 tgttattttt taaataattg aggaaataat ggctagctac agcactaacg actttaaaaa
    14461 aggtttaaaa tttattcaag atggcgaacc ttgtgtaatt gttgaaaacg aattcgtaaa
    14521 accgggtaaa ggtcaagcat ttacccgtac taaaatccgt aaattaatct ctggcaaagt
    14581 attagagatc aacttcaaat caggcacttc ggttgaagca gcagatgtag tagattctaa
    14641 ctacaactac tcttacagcg atggtgattt ctggtacttt atgcaccctg aaacctttga
    14701 acaaatctcg gtagatgaaa aagcgttagg cgacaacgta aaatggttag ttgataatgc
    14761 agaatgtatc gtgactttat ggaacggttc agcaattgca gtcacaccac caaacttcgt
    14821 tgagttagaa atcgtcgaaa ccgacccggg cttaaaaggc gatactgcag gtacaggcgg
    14881 taaaccggca acattaagca ccggtgctgt ggtaaacgtg cctttattcg ttcaaatcgg
    14941 tgaagttatc cgtgtggata cccgttcagg cgaatatgtt tctcgtgtga aataatttag
    15001 cttctctaca agtggtcgtt ttttggtcaa ttttttgcta aaaacgaccg ctcattttta
    15061 cctatcctct cagcacatca attaacgctt tcagcaaagg cgaattttgg cgacggttgg
    15121 ggtaatagag atgatagccc tcatactgca ttgcccagtc ggttaaaatc ggcacgaggc
    15181 tgccgttatc cagctcggtt tgaatgctgt cttgcggact ccaaagaatg cctaaacctt
    15241 gtttggcata gtgttgcagc aagaagccgt tattggcagt aagattgccc tgcggttgaa
    15301 atttaacgat tttcttggtg atgggatccc gaaattccca cgccagaatg ccgcccaatg
    15361 tcgcaagacg atgtttgaga caatgatgtt cggttaaatc aaagggcgtt ttcggcgtgc
    15421 cgtgctgtgc caaatagttg gggctggcag ctgcggtcat tttcacatcg ggcgaaatgc
    15481 gtagtgcaat catatctttc gccacatctg ccccaagccg aataccggca tcaaaacgct
    15541 cggcgacaat atctacaaag cgatcttccg ccactagctc caatgctact tcagggtaac
    15601 gtttgacaaa ctcagcgaat ttatctgcca gcacatcacg gaaaacgtgt tcattaccgt
    15661 taattctcag cccgcctttt aacgcattgc gaaactcact cagcccattt acttcttggt
    15721 cgatttcatc aaacagcggt aacagccgtt gatacagcct ctcccctgcc tcggtggttg
    15781 aaatgctacg agtggtgcga tgaaacagct tgatttgcaa ccgttcttca atcccccgaa
    15841 tgctgtgcga caacgccgac tgcgacacgc ccatctgtgc tgctgctttg gtaaaactgc
    15901 tcgtttgtgc taccaacaaa aaagctctta aatcgttgag gttttctttc atttaaccac
    15961 cttattaatg aatttttctc attacttcaa gcgattttac cctactaatc aaatgcaatc
    16021 tacaccgcta tactgacttc attcaatgga gaacagcaat gaaacagatc ttaattttag
    16081 gagcaagcgg tagcctagcg aatgtcgtga ttcctgaact gctaaaagac aataatgtgc
    16141 aattaaccct ttttgcccgt cgtccgcaat cagtagcgaa atttgaaact gaacgcgtca
    16201 aaattgtgca aggcgatgtg ttggatttcc acgcattaac ccaagcaatg caaggcgttg
    16261 atgccgttta cgctggactt gccggcgact taaaagcgat ggcgggaaat gtggtgaacg
    16321 caatgaatga agctcaggta aaacgcttgg tttggattag ctcaatgggg atttatggcg
    16381 aaaccggcga agatcacggg gcaattttag agccttaccg ccaatcggca gccgtgattg
    16441 aagccagcga gttggattat accgttgtcc gcccgggctg gtttaccaac ggttcggaag
    16501 tgcaatatca gctgactcac aaaggcgaac cgttcaaagg acacagcgtt tcccgcaaat
    16561 ccattgccga ttttgtggct cgcattttgc gtgaaaacag cgaaatccgt gaaagtgttg
    16621 gtattgcagc ctaacaagcg gtgaaaaacc aaagaaaatt tgcaaaaatc agaaggaaaa
    16681 cacaatgaac ttacaacaaa tccaagatcg tttagcacta aaagatttag tagatacatt
    16741 ctctaacctt gccgatgaaa aaaaggttgc cgagcagatg ccgcttttca cgcaagatgc
    16801 cgtcgtaaac acctacatcg gcggaaaatt ggtgtttgaa atgcacggcg tggcagaaat
    16861 cgaacaggtg tttaccacct ttttaacccc gtttcacagc gtttatcatt taaacggtca
    16921 gcataccgtg ccttttattg atgaaaataa cgccacagcg atcaactatt gtgcggtgaa
    16981 actggtagaa accaaagagg gcaaagagat cttgcacgat cacagcgtgc gttatgccga
    17041 tacctatgtt aaacaagagg gtaaatggcg gattgccaaa cggattgcga attttatgat
    17101 cagcgaaagc cgtgaaatca ggcaataaca agcggttaaa tttagctaaa aatttgcaat
    17161 tccattgcat cttactaaaa cagcaaaatt ctttggagga aaccgaggaa ttttgcttta
    17221 gtctttaagt ctgtttcaat taagtgtaga ataatccgtt atttttattg atacatttag
    17281 agagaactat gagcgaagaa attttaacgg cagaaactgg cactcgagcc aattttatta
    17341 cccatattat tgatgaagat ttagcaagcg gaaagcacaa caatgtttac actcgttttc
    17401 cacctgaacc aaacggttat ttgcatatcg gacacgctaa atctatctgt ttaaattttg
    17461 gtatcgctca agattataac ggtttatgta acttgcgttt tgatgatacc aacccggtta
    17521 aagaagacgt ggaatatgta gattcaatta agcaagatgt ggagtggcta ggttttaaat
    17581 gggagggtga gcctcgttat gcctcagact attttgacca gctttatggc tatgcgattg
    17641 agttaatcaa caaaggatta gcctatgttg atgaattatc gcctgatgaa atgcgtgaat
    17701 accgtggtac tttaaccgag ccgggcaaaa acagccctta ccgtgatcgt agcgtggaag
    17761 aaaacttagc gttattcgaa aaaatgaaaa acggagagtt tgcggaaggg aaagcctgct
    17821 tgagagcaaa aattgatatg gcgtccccgt ttattgtgat gcgagatccg gtgctttacc
    17881 gtgtgaaatt tgctcaccat catcaaaccg gtgataaatg gtgcatctac ccgatgtacg
    17941 attttaccca ctgcatttcc gatgcgattg agcgaatcac tcactcgcta tgtacgctag
    18001 agttccaaga taaccgccgc ttatatgatt gggtgctgga caatatttcg attgaaagac
    18061 cgttaccgca ccaatacgaa ttttcacgtt taaatttaga aggtacttta acctctaagc
    18121 gtaaattatt aaaattagtc acagacggca ttgttgatgg ttggaatgac cctcgtatgc
    18181 caaccatttc cggcttacgc cgccgtggtt ataccccagc gtctttacgt gaattttgtc
    18241 gccgtattgg ggtaaccaag caagataacg tggtggaata ctctgccctt gaagcgtgca
    18301 ttcgtgaaga cttaaatgaa aacgcaccac gcgcgatggc ggtgattaac ccggttcgag
    18361 tggtgattga aaacttcggt gaaaaggaaa tattaaaagc ccctaatcac ccaaatcgtc
    18421 cagagcttgg cgagcgtgat ttaccgttca cccgtgagat ttatattgat gaagccgact
    18481 tccgtgaaga agccaataag caatataaac gtttagtctt aggcaaagaa gttcgtttac
    18541 gcaatgccta tgtgattaaa gctgagcgtg tggaaaaaga tgcaagcggt cgaatttcaa
    18601 ctattttttg cacatacgac ccggatacat tgggcaaaaa tccggctgat ggtcgcaaag
    18661 taaaaggcgt gattcattgg gtatctgctg aagataacaa accggcagag ttccgcattt
    18721 acgaccgcct atttacagta ccaaacccgg gggcggaaga agacatcaat gcggtgctaa
    18781 atcctgaatc attagtggtt aagcatggtt ttgttgagtc aagtttagct cacgccaaac
    18841 cggaacaaga ctaccaattc gagcgtgaag gttactactg cttagacagc aaagatggtt
    18901 cggcagacaa ccttgtattt aacttaactg tcagcttaaa agaaagcgta gcgttctaat
    18961 ctaactctct tcattcaaat accagcaaat atgctggtat ttttttactc tcaaaacgac
    19021 tcatcaagca taaaaaaagc cccaattctt ggggccactc ggaaagcaaa tttatgtgtt
    19081 aggttgctag gtacaacact tattcttgtt accttaaggt taagcgaatt tgccggcagt
    19141 ttcaaacaaa atctctcaaa aatacagacc aatgtttaaa tttgtgatct cagtcacaaa
    19201 atccactttt tccaattgaa acgtttcatt ttaaattatg aggtagtgta ttatttgagc
    19261 tttctataat ct
//
LOCUS       DANLYI010000026        19210 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.026, whole genome shotgun sequence.
ACCESSION   DANLYI010000026 DANLYI010000000
VERSION     DANLYI010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 19210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19210
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.026"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            complement(66..2978)
                     /gene="rapA"
                     /locus_tag="PXD81_000699"
     CDS             complement(66..2978)
                     /gene="rapA"
                     /locus_tag="PXD81_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HDL5536752.1"
                     /translation="MFLVGQRWLSESENNLGLGIVTSVDHRTVTINFPAAEEERVYAI
                     AVAPLTRVQFQAGDRINSIDGWQLEVSEVVENQGAVIYLGKRVDNGEEAVLPEMLLDH
                     KISFSKPQDRLFSAQIDRSDRFALRFNALQHQQAQYQSPLRGLRGIRASLIPHQLHIA
                     KEVGQRLAPRVLLADEVGLGKTIEAGMILQQQLFSGRAERVLVLVPESLQHQWLVEML
                     RRFNLKFSLFDEERCSDFDKQDENGDDVSENPFESEALVITSLNWLETSPQRANQVLA
                     ADWDLLIVDEAHHLEWSETESSIGYQLVERLSQQIPAVLLLTATPEQLGQESHFARLA
                     LLDSDRFYDYAEFVQEQAQYKPVADAVSSLLSDKPLSNDEQNAIAELLSEQDTEPMFR
                     AINSKKSDENDRLQARQELISELIDRHGTSRVLFRNTRQGVKGFPHREYHQISLEMPK
                     QYQNALKVMGMMGSVSQSDLLYPEYLFQRMNPNAKWAEFDPRIDWLITFLKNHRDEKV
                     LVICKQADTAIALEQVLREREAIRSAVFHEKMSIVERDKAAAYFAQQEEGAQVLISSS
                     IGSEGRNFQFAKDLVLFNLPDNPDLLEQSIGRLDRIGQKNDIQIYVPCFNLSAQQMLA
                     EWYHKGLNAFEETCPMGTAIFRELGEELQQFLQKPELSAQFDDFVAKTYKRQQYLKAE
                     LEKGQDRLLELNSNGGEAAQQLANDIAKEDNNPQLVNFALSLFDVIGVEQEDLGEQSI
                     VISPTGHMLVPDFPGLSEDGTTITFNRELALMREEVEFLTWDHPMIRNGIDLITSGDI
                     GKSAISLLVNNKLPAGTLLLEALYVVEAQAPKGLNLTRFLPPTPIRVLVDNKGNNIAP
                     QVSFAGLERQLKPVNKQMANKIAKMARADIEKLIKASEKAVEPQVQQVIETAKFEANS
                     KLKAELHRLESLKMVNKNIRADEVEALEQQLNASLAQLETANYRLDSLRVIVSNKA"
     gene            complement(3139..3438)
                     /locus_tag="PXD81_000700"
     CDS             complement(3139..3438)
                     /locus_tag="PXD81_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5536753.1"
                     /translation="MRFLPSEIYQPRGELVKADRQGNGEFEVEYRVSGNDVRGLAKSA
                     IAHAKRKGFHLVESDIHRDDADLKFKRGDQELDIEIEVKGRNRIEYKADLDLDKN"
     gene            complement(3559..4884)
                     /locus_tag="PXD81_000701"
     CDS             complement(3559..4884)
                     /locus_tag="PXD81_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase
                     family protein"
                     /protein_id="HDL5536754.1"
                     /translation="MISSVWNERYLADKKREQDHRSPFQRDRARLLHSEAFRCLQAKT
                     QIHAVGEDDFYRTRLTHSLEVAQIGSSLREKLLADVEGFQAVCSEQEFAKNMANLTAL
                     LPDHSLVESLCFAHDLGHPPFGHGGEMALNYKMYAFGGFEGNAQSFRIMTQLEPYTET
                     AGMNLTRRTLLGVMKYPALLSQTSPIEPAEMSEARHINLHHFPQSKGIFDDNNHFFEW
                     VLAPLSEKDRRQFCTVQPNQDPKQPNKTQYKSLDCSIMELADDIAYGVHDLEDAIVGG
                     MVTPQSWQNAEKLLAECQSDWVKQRLPEIREKLFSQHRYERKDVIGALVNHFITNVRW
                     KALPEFDEPLLRYNAYLPESVACVLKILKDFVYQYVICDAKTQRVERKGQRILMELFD
                     ILSSDPMRLLPNNVRERWKNAEESKRARVICDYLAGMSDGQAFKLFESL"
     gene            4969..6288
                     /gene="rlmD"
                     /locus_tag="PXD81_000702"
     CDS             4969..6288
                     /gene="rlmD"
                     /locus_tag="PXD81_000702"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HDL5536755.1"
                     /translation="MAIFYSEKSAKKPIKSPACQKATIQSLDYQGLGIAKINGKTWFI
                     ENALPDEQVEFKVLEEKRQYGRGQAVKILKKSANRRMPSCELYGQCGGCQMQHISLEL
                     QRKAKQQALFQRLQKLQTHAIDVQPMIIGNDKHYRRRAKLSIAIQQGKAVIGFRLQNS
                     NQIIPLAHCEVLVEPLANLLPKLKHLIENWQQPKKLGHIELVSADNTIALFFRHLGAL
                     SAQDNHNLRQFATQEQLSLFVMTSENHIEHLCGERPYYQIQGLKLHFSIRDFIQVNAT
                     LNEKMVEKALEWLELSNQDRVLDLFCGMGNFTLPIAERAKSVVGVEGVEPMVQQAREN
                     AVTSGLKNVEFYQTNLDEPFSDKPWAKEPFNKVLLDPARNGALFCLDHLTSLNPERIV
                     YVSCNPATLVRDAEKLLQLGYRLEKAAMIDMFPHTGHLESISLFLKS"
     gene            complement(6428..7609)
                     /locus_tag="PXD81_000703"
     CDS             complement(6428..7609)
                     /locus_tag="PXD81_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bcr/CflA family multidrug efflux MFS
                     transporter"
                     /protein_id="HDL5536756.1"
                     /translation="MESRRPSLSFFIILGMMAMLPPLAIDMYLPSFLDIARDLAVSQE
                     KVQTTLAVFTLGFGAGQLFWGPMADSFGRKIIILIGLVGSAIAAYFLTQVQNIETFYL
                     LRLVQGLCAAAPAVVLGALVRDLFERNLFAKLMSVIMIISMLAPLLAPIVGGYIAKYF
                     HWHSIFYVLVAMGILCVVLISWKIPETLAPEKRLPLSFGKTLSNFAILLKHRPTLGYV
                     LIGGLTFAGIFCFLTSGSIVYIGLYGVSQENFGYFFGLNMIVMVTMTAINSKMVVKVG
                     SETMLRIALAMQLLAGIWLALVAIFDLGFWAMAIGIPLYIGMLSTIGSNASAAILEQF
                     PQMAGTANGVAGTARFGVASLVGAGLSHIAVTSAAPMLLAMALCVMLAGVVYYFLCYK
                     R"
     gene            complement(7599..8300)
                     /gene="rsuA"
                     /locus_tag="PXD81_000704"
     CDS             complement(7599..8300)
                     /gene="rsuA"
                     /locus_tag="PXD81_000704"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HDL5536757.1"
                     /translation="MRLDKFIAENTGLTRSQAAKVLKSGVVTVNGNIEKSGAAKISQE
                     DEIYYEDQKLEWVEEGQYFMLYKPQGYICSHDDGEYPTVFQFFDYPLMTKLHTAGRLD
                     VDTTGLVLLTDDGKWSHRITSPKHHCEKTYLVTLADPVEDFYAQRLAEGILLRGEKEP
                     TLPAQLEILDDYNVNLTISEGRYHQVKRMFAALGNKVEALHRWRIGDVVLDERLEEGE
                     FRSLTSEEIASFNGK"
     gene            complement(8545..9516)
                     /gene="menC"
                     /locus_tag="PXD81_000705"
     CDS             complement(8545..9516)
                     /gene="menC"
                     /locus_tag="PXD81_000705"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HDL5536758.1"
                     /translation="MRQVKLYRYQLPIQTGVVLRKQPLKERQGVLVCLQEQEKVGWGE
                     IAPLPSFSQESLEQAENQAVNWLKAWQKGEPETLEQLFPSVAFGLSCALAELNNELGE
                     QANNQSAVLCYGDLEKLHTAFSQHTNPIGKIKIGLNPEQEGEQANLLLHTFPNLRLRL
                     DANRLWSLEQAVKFAEKIANEHKPRIQFIEEPCQALELSRQFATQTDIAIAWDETVRE
                     ANFLVKKEPNLTAIIIKPTLVGSLEKCVNLIQQAQKQGLVAVISSSLESSLGLTQLAR
                     IAQQYTPNSVPGLDTLNLMQHQLLRDWHDSSLPLVGLESEFVREVVV"
     gene            9728..11806
                     /gene="metG"
                     /locus_tag="PXD81_000706"
     CDS             9728..11806
                     /gene="metG"
                     /locus_tag="PXD81_000706"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HDL5536759.1"
                     /translation="MANQKRKMLVTCALPYANGAIHLGHMLEHIQADIWVRFQRMRGN
                     EVHFVCADDAHGTPIMLNADKLGITPEQLIEKAKADHEADFASFNISFDNYHSTHSEE
                     NRQITTDIYKTLKANGFIKSKVISQLFDPEKNMFLPDRFVKGTCPKCKAEDQYGDNCE
                     VCASTYSPMDLINPRSAVSGATPVVKESEHFFFDLPSFEGMLKEWTRSGSLQPEIANK
                     MQEWFESGLQQWDISRDAPYFGFEIPDAENKFFYVWLDAPIGYIASFKNLCDRAGIDF
                     DEFWQKDSTTELYHFIGKDIVYFHSLFWPAMLEGSGYRKPTNVFAHGYVTVDGAKMSK
                     SRGTFIQANTYLKHLDPECLRYYYAAKLNDRIEDLDFNLDDFVQRVNSDIVNKLVNLA
                     SRNASFIAKRFEGKLAEKLDDEALFNEFTAQAETIANFYENREFNKAIRAIMELTDKA
                     NKYIDDKAPWVIAKEEGKEAELQAVCSMGIELFRVLMSYLKPVLPKLAERAEAFLQSE
                     LRWDNIATPLFGHTVAPFKSLFSRLEKKQIDAVIDETKALFAEANKKTENQTKGTQKV
                     EQQAVEKQENIAVEPISPEITIDDFAKLDLRAAKVLNCEAVPKSDKMLRFELDLGDHQ
                     RQVFSGIKAAYPNPEALIGRFVIVIANLAPRKMSFGMSEGMILSAGTGGANLFLLDAD
                     SGVQPGMQVK"
     gene            11816..12232
                     /gene="ndk"
                     /locus_tag="PXD81_000707"
     CDS             11816..12232
                     /gene="ndk"
                     /locus_tag="PXD81_000707"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HDL5536760.1"
                     /translation="MIQQTLSIIKPDATRRNLIGKILAQLEENGLTIKALKKVHLTRE
                     QAEGFYAEHKGKDFFEPLVEFMTSGSIVVSVLEGENAISHYRALMGATNPDNREKGTI
                     RDLYALSMRENSVHGSDSEESAKREIAYFFVPSEIV"
     gene            complement(12288..12764)
                     /gene="greA"
                     /locus_tag="PXD81_000708"
     CDS             complement(12288..12764)
                     /gene="greA"
                     /locus_tag="PXD81_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HDL5536761.1"
                     /translation="MKQIPMTVRGAEQLREELDFLKNVRRPQIIDAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIQEIEGKLGNAQIIDVTKMANNGKVIFGATVQLVNADTDEE
                     VTYRIVGDDEANIKEGLISVNSPIARGLIGKEVDDSVSITTPGGKVEFDIVGVEYI"
     gene            12861..14306
                     /gene="dacB"
                     /locus_tag="PXD81_000709"
     CDS             12861..14306
                     /gene="dacB"
                     /locus_tag="PXD81_000709"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005615286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HDL5536762.1"
                     /translation="MIFKNFTRAFLNKFILSTLLIPSITQAEINTEELLNTLPAGTSV
                     SFIAKNLDTNQIITQYQSDIFMLPASTQKVFTALAAKLTLNDDFRFQTALLTNGKVEN
                     GVLKGNLIARFSGDPELTSGQIYQLMSKLKQQGINKIEGDLILDPSVFASHDKASGWI
                     WNDLTMCFNAPPAAINVDHNCFYVTLNADQPIGEFAKVNVPSAYPVQVFSSAYIVEPK
                     EAPFCQLDVVVHDNNRYQIKGCMARQSQPFGLSFSVQDPTNYGANMLKAQLKSLKIAF
                     NGQVKEPLTAQNGTLLAEHYSEPLPVLLKKMMKKSDNQIADALFRTVANKQHNRPASF
                     QLGSYVIRQLLKTKANIDFKNSVVADGSGLSRHNQVSSRTMLETLEYIAQNEESLKLF
                     ETFPIAGVDGTISGRGSISTEPLAKNLIAKTGSLKGVYNLAGFMKNARGERIAFVQFI
                     NGYSTGELESKTKRAPLNTFENKFYMALFNE"
     gene            complement(14393..14662)
                     /locus_tag="PXD81_000710"
     CDS             complement(14393..14662)
                     /locus_tag="PXD81_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005601284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDL5536763.1"
                     /translation="MINHNLADFVFEYFTENNLKAGTLRYRYIKENVIDAYSTRVDDA
                     FQGKGIAGELYNALIAFAQEKGLKIKPSCSYIEVKMQRSHRDLIA"
     gene            14834..16423
                     /gene="purH"
                     /locus_tag="PXD81_000711"
     CDS             14834..16423
                     /gene="purH"
                     /locus_tag="PXD81_000711"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005821463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HDL5536764.1"
                     /translation="MAIQQALLSVSDKKGIVEFARGLSARGVKLLSTGGTAKLLAEAG
                     LLVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGTDDEVMNQHGIERIDMVVVNLY
                     PFAQTVANPNCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVNNNDFDAILAEMDQHQ
                     NSLTLETRFNLAIKAFEHTAQYDSMIANYFGQKVPPYQGAEEEDLTQPSGQFPRTLNL
                     NFVRKQTMRYGENSHQNAAFYVENELKEASVSTAKQLQGKALSYNNIADTDAALECVK
                     EFSEPACVIVKHANPCGVALGKNILDAYNRAYQTDPTSAFGGIIAFNRELDGETAQTI
                     ADRQFVEVIIAPTVSASAKEVLATKKNVRVLECGEFSEAVKRLDFKRVNGGLLVQDAD
                     QGSVSIDDLKVVSERKPTEAELKDLLFCWKVAKYVKSNAIVYAKDGQTIGIGAGQMSR
                     VYSAKIAGIKAEDEGLQVVGCVMASDAFFPFRDGIDAAAKVGITCVIHPGGSMRDQEV
                     IDAANEHGMAVVLTGMRHFRH"
     gene            complement(16478..17854)
                     /locus_tag="PXD81_000712"
     CDS             complement(16478..17854)
                     /locus_tag="PXD81_000712"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000626422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HDL5536765.1"
                     /translation="MISVFDMFKVGIGPSSSHTAGPMKAAKEFVDELQNRKFLNKIDR
                     LRVDVYGSLSLTGRGHNTDIAIIMGLMGYLPHNVDIERIEQTIENLKQNKQLIIAESS
                     VEPKVIAFDFYEDMPFHRDFLPRHENGVKFSALSGEELLFQNTYYSIGGGFIVDEANF
                     EQVKNSDLSVPFPYQNAADLLAHCKNNGLPLSSLVWKNELALRSRVEISDYLAMIWNT
                     MEACIERGLNTEGLLPGPLKVVRRASGLRRMLEANSKFNTDPMQIIDWVNMFALAVNE
                     ENAAGGRVVTAPTNGACGIVPAVLAYYQKFIAQLNQDTIERYLLTTSVIGSLYKMNAS
                     ISGAEVGCQGEVGVACSMAAAGLTEIMGGSAEQVCMAAEIAMEHNLGLTCDPVGGQVQ
                     VPCIERNAIASVKAINASRMALRRTSSPQVSLDKVIETMYETGKDMNAKYRETSTGGL
                     AIKILPCD"
     gene            complement(17911..19137)
                     /locus_tag="PXD81_000713"
     CDS             complement(17911..19137)
                     /locus_tag="PXD81_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005601171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
                     /protein_id="HDL5536766.1"
                     /translation="MAMPSQRFSLTWVLNLFGTAVGAGVLFLPINAGMGGFYPLIIMT
                     LLVGPMTYLAHRGLTRFVLSSKYKGSDITAVVREHFGEQAGKLITLLYFFAIFPILLI
                     YGVGITNTVSSFMENQLHIAPPSRAVLSFMLIAAMIGVMLLSEQVMLKITTCLVYPLV
                     LILLGLSIYLIPQWNTAALQQMPTTGDFLTTLWLTIPVLVFAFNHSPAISSFALSQQK
                     YYQDDKKAEIESAKVLRSTAFILVLFVMFFVFSCVLTLTPEELAQAKVQNISILSYLA
                     NKFDNPIISYFGPLVAFLAIGSSFFGHYLGAREGLEGLVNQMRKEPIDPSKFRKITAI
                     TFLIILWIVATINPSILGFIESLGGPIIAMILFIMPVYAVYKVPALARFKGEFGHLFV
                     LVMGCIAISAIVYGLL"
BASE COUNT         5671 a         4390 c         3799 g         5350 t
ORIGIN      
        1 cacaaaatag aaaagtgatt ttttcgacac agatcgaaaa aatcactttt cgtacttgca
       61 aaaaattacg ctttattact tacaatcact cgcaagctat ctaagcgata atttgccgtt
      121 tcaagttggg caagggaagc gttaagctgt tgttctaagg cttcaacttc atcggctcga
      181 atatttttat tcaccatttt gagtgattcc aagcggtgta attctgcttt taatttgcta
      241 tttgcctcaa atttggcagt ttcaatcacc tgttggacct gaggttctac cgctttttca
      301 ctcgctttaa ttaatttttc aatatccgct cgagccattt tggcaatttt atttgccatc
      361 tgtttattca ccggttttaa ttggcgttca agcccggcaa aagagacctg cggagcaata
      421 ttgttgcctt tgttatccac taaaacccga atcggggttg gcggtaggaa acgggttaaa
      481 tttagccctt tcggtgcttg tgcttccacc acataaagcg cctcaagtaa taatgtaccg
      541 gcaggcaatt tattattcac cagcaatgag attgctgatt tgccaatatc gccggaggtg
      601 attaaatcaa taccattgcg gatcatcggg tgatcccaag tgagaaattc cacctcttcc
      661 cgcattaacg ccagttcacg attaaaggta atggtcgtgc catcttccga tagccccggg
      721 aaatcaggca caagcatatg tccggttggg ctaatcacaa tgctctgctc gcctaaatct
      781 tcctgttcaa cgccaatcac atcaaacaaa ctcaaggcaa aattaaccaa ctgcggatta
      841 ttatcttctt tcgcaatatc attagcgagc tgttgagctg cttctccgcc attagaattc
      901 agttctaata atctgtcctg tcctttctcc agttcggctt tcaaatactg ttggcgttta
      961 taggttttcg caacaaaatc atcaaattgg gctgaaagtt caggtttttg caggaattgt
     1021 tgcaattctt cacctaattc acggaagatc gctgttccca tcgggcaggt ttcttcaaac
     1081 gcattcaagc ccttgtgata ccactctgcc aacatctgtt gagccgataa attaaagcaa
     1141 ggcacataaa tctgaatgtc atttttctgt ccgatacgat ctaatcgccc aatactttgc
     1201 tccaataaat ccgggttatc cggcaaatta aacagcacta aatctttagc aaattggaaa
     1261 tttcgccctt ctgaaccgat gcttgaacta atcagcacct gagcgccttc ttcttgttga
     1321 gcaaaatagg cggcagcttt gtcacgttca acaatcgaca ttttctcgtg gaaaacggca
     1381 gaacgaatcg cctcacgctc acgcaaaact tgctccaaag caatcgcagt gtccgcctgt
     1441 ttgcagatca ccagcacttt ttcatcacga tgatttttta agaaagtgat gagccaatca
     1501 atccgagggt cgaattcagc ccatttcgca ttcgggttca ttcgttggaa caaatattca
     1561 ggatagagca aatcactttg gctcacactc cccatcattc ccatcacttt taacgcattt
     1621 tggtattgtt tcggcatttc taggctgatt tggtggtatt cccgatgtgg gaagcctttc
     1681 actccctgac gggtatttct aaacaacacc cggctggttc catggcggtc aattagctca
     1741 ctaatcagct cttgacgggc ttgcaagcgg tcattttcgt ccgatttttt gctattaatt
     1801 gcacggaaca tcggctcggt atcttgctcg cttaacagct ccgcaatcgc attctgctcg
     1861 tcattactta gcggtttatc gcttaataag ctactcaccg catctgccac cggtttatac
     1921 tgtgcttgct cctgtacaaa ctcggcataa tcataaaaac gatccgaatc taacaacgcc
     1981 aagcgggcaa aatggctctc ttgccctaat tgctccggtg ttgccgttaa aagcaagacg
     2041 gcaggaattt gttgagataa acgctctacc aactgatagc cgatagaaga ttcggtttcc
     2101 gaccattcta aatggtgagc ttcatcaaca attaatagat cccagtcggc agcaagcact
     2161 tgattggctc gttgcggtga ggtttccagc cagtttagtg aagtaatgac taacgcttcg
     2221 ctctcaaatg gattttcact tacatcatcg ccattttcat cttgtttatc aaaatcacta
     2281 caacgttctt catcgaataa cgagaatttc agattaaaac ggcgaagcat ttccaccaac
     2341 cattggtgtt gcaaactttc cggcactaat accaatactc gttctgctct gcccgaaaat
     2401 agctgttgtt gcaaaatcat accagcttca atagttttac ccagtcccac ttcatcggct
     2461 aatagcacac gaggagcaag gcgttgcccg acttcttttg caatatgtaa ttgatgtgga
     2521 atcaggctgg ctcgaatgcc tcgcaaacca cgcagtggcg actgatattg cgcttgctgg
     2581 tgctgcaaag cgttaaagcg taatgcaaag cgatctgagc gatcaatctg agccgaaaaa
     2641 aggcggtctt gcggtttgct gaaactgatt ttatgatcga gcaacatttc aggcagtacc
     2701 gcttcttcac cgttatccac ccttttgccc aaatagatta cggcaccttg attttccaca
     2761 acctctgaaa cctctaactg ccagccatct atactgttaa ttctatctcc ggcttgaaac
     2821 tgcactcgag ttaaaggtgc aactgcaatt gcatagaccc gctcttcttc cgctgccggg
     2881 aaattgatag tgacagtgcg atgatccacc gaagtcacga ttcccaaacc taaattattt
     2941 tccgattcac ttagccaacg ctgaccgact aaaaacattt tttcctctaa gtaataagta
     3001 aaagcatcat tattgtcatt taaacggcta ttttaccaaa ttccgggacc ggtcggcttg
     3061 agtatttttg caaaaaagca caataaaaag accgcttgtg cggttaaatc acacaagcgg
     3121 tcgttttaag gggtaaattt agtttttatc taaatctaga tctgccttat attcaatccg
     3181 atttctacct tttacttcaa tttcaatatc cagctcttgg tcaccacgtt tgaattttaa
     3241 atcagcatca tctctatgaa tatcagattc tactaaatgg aaacctttac gtttagcgtg
     3301 ggcgatcgca cttttcgcta agccacgaac atcattacca ctgacacgat attctacttc
     3361 aaactcacca ttaccttgac gatctgcttt aactaattcg ccacgaggct gataaatttc
     3421 acttggaaga aaccgcaaat gctgttgcag aagccaaagt tgctaatact aaaagacctt
     3481 tagaaacagt tttcattgaa atctcctatt aaataaatga aacctattac tgagaccttg
     3541 caaaattaac aaagttcatt ataaactttc aaaaagttta aacgcctgcc catcagacat
     3601 accggctaaa tagtcgcaaa ttacccttgc acgcttagat tcttccgcat ttttccaacg
     3661 ttcccgcaca ttattaggga gtaatcgcat cggatcactg cttaaaatat cgaacaattc
     3721 cattagaatg cgttgcccct tgcgttccac ccgctgggtt ttggcatcac aaatcacata
     3781 ttgatacaca aaatctttca agattttcag cacacaggca acactttccg gtaaataggc
     3841 attataacgt agcaacggct cgtcaaattc aggcagagct ttccaacgca cattggtaat
     3901 aaaatgattc actaatgccc cgatcacatc ttttcgctcg tagcggtgct gagaaaagag
     3961 cttttcccga atctccggca ggcgttgttt cacccaatcg gattggcact ccgccagcaa
     4021 tttttccgca ttttgccacg attgcggtgt gaccattcca cctacaatcg catcttccaa
     4081 atcgtgtacg ccataggcaa tatcgtccgc tagttccatt atgctgcaat cgagagattt
     4141 atattgcgtt ttgtttggct gtttcggatc ttgattgggc tgaaccgtgc aaaattggcg
     4201 tcggtctttt tcacttaacg gggctaatac ccactcaaaa aagtggttgt tgtcatcaaa
     4261 aatccctttg ctctgtggaa aatggtgcaa attaatatgc cgagcctcgc tcatttctgc
     4321 cggctcaatc ggcgaggttt gagacaagag tgccggatat ttcattaccc ccagtaaggt
     4381 gcgacgggtc agattcattc cggctgtttc ggtgtaaggc tcaagctggg tcataatccg
     4441 aaaggattga gcattaccct cgaagccgcc aaaggcgtac attttataat ttaacgccat
     4501 ttcaccaccg tgcccgaacg gagggtgccc gagatcgtgg gcaaaacaaa gggattccac
     4561 caagctatga tccggcaata aagcggtcag atttgccata ttttttgcaa attcctgctc
     4621 cgaacagacc gcttgaaagc cttccacatc cgctaataat ttttctcgca aactgctacc
     4681 gatttgagcc acttccaacg aatgggttaa acgggttcga taaaaatcat cttcccccac
     4741 ggcgtgaatt tgcgttttcg cctgcaaaca gcgaaaagcc tccgaatgca ataaccttgc
     4801 ccgatcccgc tgaaaaggcg agcggtgatc ttgttcccgt tttttatccg ccaaataacg
     4861 ttcattccaa accgatgaaa tcatattgcc tccattttcc gccaactgct tttcttatat
     4921 aatctcccca tttcgacact ttatatcata acaattcatc tgaaaacaat ggctattttt
     4981 tattctgaaa aatcggcaaa aaaaccgata aaatcacccg cttgtcaaaa ggcaactatt
     5041 caatcgttag attatcaagg cttagggatt gccaaaatca acggcaaaac ttggtttatc
     5101 gaaaatgccc tgcctgatga gcaggtggaa tttaaagtgt tggaagagaa acgccaatac
     5161 ggacgtggac aagcggttaa aattcttaaa aaatctgcaa atcggcgaat gccaagctgc
     5221 gaactttacg ggcaatgcgg tggctgccaa atgcagcata tttcgcttga actgcaacgt
     5281 aaggcaaaac aacaggcttt attccaacgc ttacagaaat tgcaaaccca cgctattgat
     5341 gttcagccga tgattatcgg caacgataaa cactaccgcc gtcgggcaaa attaagcatt
     5401 gcgatccagc aaggcaaggc ggtaatcggc tttcgtttgc aaaattccaa ccaaattata
     5461 ccgcttgcac actgtgaagt gctggttgaa ccgcttgcca atttgctccc aaaattaaaa
     5521 cacctgattg aaaactggca acagccgaaa aaattggggc atattgagct ggtcagtgcg
     5581 gacaatacca ttgccctatt tttccgccat ttaggggcat tatcggcgca agataaccac
     5641 aatttgcgtc aattcgccac acaagagcaa ttatctcttt ttgtgatgac tagcgaaaat
     5701 cacattgagc atctgtgcgg tgaacgcccg tattatcaaa ttcagggtct aaagttgcat
     5761 ttttcaattc gggattttat tcaggtgaat gccacgctca atgaaaaaat ggtggaaaaa
     5821 gcccttgagt ggctggaatt atccaaccaa gatcgagtgt tagatctctt ctgtggaatg
     5881 gggaatttta ccttgcctat tgctgagcga gcgaaatcgg tggtcggtgt ggaaggggta
     5941 gaaccaatgg tgcaacaagc ccgagagaat gcggtgacaa gcggtctaaa aaatgtggaa
     6001 ttttaccaaa ccaatttaga tgagccgttt tctgacaaac cttgggcaaa agagccgttt
     6061 aacaaggtac tactcgaccc ggcacgcaac ggagcactat tttgcctaga tcacttgaca
     6121 tcactcaatc ctgagcggat tgtttatgtc tcttgcaatc cggcaacact ggtgcgagat
     6181 gccgaaaaac tgttgcaact aggctacagg ctagaaaaag cggcaatgat tgatatgttc
     6241 ccccacaccg ggcatttgga atctatttcg ctgtttttaa aatcttagta gatattgccc
     6301 cgatggtgcg agcgtccacg ctcgtaccac acaagcggtt aattctgcct aaaaatttgc
     6361 aaaaaatgta ctattattaa ccgcttgtag aaatttgttg ggttttcgca agctaacccc
     6421 aacctagtta tcgcttataa cacagaaaat aatacaccac accggctaac ataacgcaca
     6481 atgccatcgc caaaagcatt ggggcagcac tggttacagc aatatgtgaa agccctgctc
     6541 caacaaggga agcaacacca aaacgagccg tacctgctac tccgttagcc gtgcctgcca
     6601 tttgtggaaa ttgctctaaa atggcggcac tggcattact accaatggtg gaaagcattc
     6661 cgatataaag cggaataccg attgccattg cccagaagcc taaatcaaaa atcgccacta
     6721 aagccagcca aatgccggct aacagttgca tagctaaagc aattcgtaac atcgtttcag
     6781 agccgacttt cactaccatt ttgctgttaa tcgccgtcat cgtcaccatc acaatcatat
     6841 tcagcccgaa gaaatagccg aaattttctt gcgacacgcc ataaagccca atatagacaa
     6901 tcgagcctga ggttaaaaaa cagaaaatac ctgcaaaggt taagccgcca atcagcacat
     6961 aaccaagagt aggcctatgt ttcagtaaga tggcaaaatt gcttaaagtt ttgccaaaac
     7021 ttaacggcag gcgtttttcg ggggctaatg tttcagggat tttccaacta attaatacca
     7081 cgcacaaaat ccccatcgcc actaacacat agaaaattga gtgccagtgg aaatatttgg
     7141 caatataacc gccaacaatc ggtgcaagca atggggcaag catagaaata atcataataa
     7201 ccgacatcaa tttggcaaat aaattacgct caaacagatc tctcactaat gctcccagca
     7261 ccaccgccgg ggctgccgca cataagccct gtactaacct tagcaggtaa aatgtttcaa
     7321 tattctgaac ttgggtcaga aaataagcgg caatcgccga gccgactaat ccgattaaaa
     7381 taatgatttt ccgcccaaag ctgtctgcca tcggtcccca gaatagttgc cctgccccaa
     7441 aacctagggt aaataccgcc aaggtggttt gcactttttc ttgcgaaacc gccaagtcac
     7501 gggcaatgtc taaaaatgag ggcaaataca tatcaatggc taatggtggc aacattgcca
     7561 tcatacctaa tatgatgaaa aagctcaggc ttggtcttct actttccatt aaaactggct
     7621 atctcctctg aagtgagcga gcggaattca ccttcttcca acctttcatc taacaccaca
     7681 tcgccaattc gccagcggtg caatgcctcg actttattcc caagggcggc aaacattcgc
     7741 ttaacttgat gataacgccc ctcactaata gttaaattca cattgtaatc gtctaaaatt
     7801 tcaagttgag ccggcaaggt tggctctttc tcgccacgca ataaaattcc ttctgccaat
     7861 cgctgtgcat aaaaatcttc caccggatca gcaagcgtca ctaagtatgt cttttcacaa
     7921 tggtgcttgg gcgaggtaat acggtgcgac catttgccgt catcggttaa cagcaccagc
     7981 cctgtggtat ccacatctaa ccgccctgcg gtatgcaatt tagtcattaa cggataatca
     8041 aagaattgaa acaccgttgg gtactcacca tcatcgtgtg aacaaatata gccctgtggc
     8101 ttatagagca taaaatactg cccctcttcc acccactcta atttctgatc ttcgtaataa
     8161 atttcatctt cttgactgat ttttgccgca ccactttttt caatattgcc gtttaccgtc
     8221 accacaccac ttttcagcac tttagccgct tgtgaacggg tcaaaccggt attttcggcg
     8281 ataaatttgt ctaaacgcat actattttta taccataaat tactttaccc tctcccctcg
     8341 tgggagaggg acagattttt cttcgtctag aagaaaaatc agggagaggg gggaaatacg
     8401 tcttcgtcca gaagaaaaat cagggagagg ggaaatgtgc aaactttttt agaaaaatga
     8461 ccgcttcccc tctctctgct tatttcgctg aacgctcata agcggtctct ctcccacaag
     8521 gggagagagt gtttaattgg gagattatac cacaacttcc ctaacaaatt cgctttctag
     8581 cccgaccaac ggcaagctag agtcgtgcca atctcgcaat aattggtgtt gcattaaatt
     8641 taacgtatcc aaccccggca cactgtttgg ggtgtattgt tgggcgatac gagccagttg
     8701 ggttaagccc aaactgctct caagacttga actaatcacc gctactaatc cttgcttctg
     8761 tgcttgttga attaaattca cgcatttttc aagcgaacca accagcgtag gtttaatgat
     8821 aatagcggtc aaatttggct ctttttttac caaaaaattc gcctctctca cggtttcgtc
     8881 ccacgcaatc gcaatgtcgg tttgtgtggc aaattggcgg gaaagttcga gcgcttggca
     8941 aggttcttcg ataaattgaa tacggggttt atgttcattt gcaatttttt cagcaaattt
     9001 gaccgcttgt tccaagctcc aaagacggtt ggcatcaagc cttaggcgta aattcggaaa
     9061 agtatgcaac agcaaatttg cctgctcgcc ttcctgttca ggattgagac cgattttaat
     9121 tttaccgatt gggttcgtat gttggctaaa tgccgtgtgt aacttttcca aatcgccata
     9181 gcacagcacc gccgattggt tattcgcttg ctcgcctaat tcattattca gttcagccaa
     9241 cgcacagctt agcccaaagg caacagacgg gaaaagctgc tctaacgttt caggctctcc
     9301 tttctgccac gcctttaacc aattcacagc ttggttttca gcttgttcta ggctctcttg
     9361 gctaaaactt ggaagcggtg caatttcgcc ccagccgact ttctcctgtt cttgcaaaca
     9421 caccaacacc ccttgccttt ctttcaaagg ttgcttgcgt aacactacac cggtttgaat
     9481 ggggagttgg tagcggtata actttacttg tcgcatacta tttttatcca aaataggttt
     9541 agaggcgaaa catctttcgc ccacatcatc atttgggaaa ttgaggcgaa aaatgtttcg
     9601 cctctagcgc atcatattct accgcttgtt agcccaaagc tctaataaat tcacgccaac
     9661 tggtaaacct gccacttttg ggctaaaata gacggcaatt tttaccctaa ttttaatgag
     9721 aaaaagaatg gctaaccaaa aacgaaaaat gcttgttact tgtgccttgc cctatgctaa
     9781 cggtgcaatt catttaggtc atatgcttga acatattcaa gcagatattt gggtgcgttt
     9841 tcaacgtatg cgtggcaatg aagtccactt tgtctgtgct gatgatgccc acggcacgcc
     9901 tattatgctt aatgccgata aattaggcat tacccctgag cagttaatcg aaaaagccaa
     9961 agccgatcac gaagcggatt ttgccagttt taatatcagc tttgataatt accattccac
    10021 ccacagcgaa gagaaccgcc aaatcactac cgatatttat aaaaccttaa aagcgaacgg
    10081 ttttattaaa agcaaagtga tttcacaact gttcgaccca gaaaaaaata tgttcttgcc
    10141 ggatcgcttt gtgaaaggga cctgcccgaa atgtaaagcc gaagatcaat atggcgataa
    10201 ctgtgaagta tgtgcctcta cctatagccc gatggattta atcaaccctc gctcggcagt
    10261 ttccggtgca actccggtgg taaaagaatc agaacatttc ttttttgacc tgccaagctt
    10321 tgagggaatg ttgaaagagt ggactcgttc cggctcactc caacctgaaa ttgccaataa
    10381 aatgcaagaa tggtttgaaa gtggtttaca gcaatgggac atcagccgtg atgcccctta
    10441 tttcggtttt gaaattccgg acgcagaaaa caaattcttc tacgtttggt tagatgcccc
    10501 tattggttat atagcatcgt ttaaaaattt atgcgaccgt gcaggcattg attttgatga
    10561 gttttggcaa aaagattcca ccaccgagct ttatcacttc atcggtaaag acattgttta
    10621 cttccacagc ctgttttggc cggcaatgtt ggaaggcagc ggctaccgca aaccgaccaa
    10681 tgtgtttgct cacggttatg tgacagtgga cggggcgaaa atgtcgaaat ctcgtggtac
    10741 attcattcaa gccaacacct atttaaaaca tctcgatccg gaatgcttac gctattacta
    10801 tgcggcaaaa ttaaacgatc gcattgaaga tttagatttt aatttagacg acttcgtgca
    10861 acgtgtaaac agcgatatcg taaacaaatt agtgaatctt gcttctcgca acgccagttt
    10921 tatcgctaaa cgctttgagg gcaaattagc tgaaaaatta gatgacgaag cgttatttaa
    10981 tgaatttacc gctcaagcag aaaccatagc gaatttctat gaaaaccgtg aatttaataa
    11041 agctattcgt gccattatgg aattaaccga taaagccaat aaatacattg acgacaaagc
    11101 cccttgggtc atcgccaaag aagaaggcaa agaggctgaa ttacaagcgg tttgttcaat
    11161 gggcattgag ttattccgtg tgctgatgag ctacctaaaa ccggtgttac caaaactggc
    11221 agaacgtgcc gaagcattct tacaaagcga attgcgttgg gataatatcg ctaccccatt
    11281 attcgggcat accgtggcac cgtttaaatc gctattctca cgccttgaga aaaaacagat
    11341 tgatgcagtg attgatgaaa ccaaagcctt atttgccgaa gcgaataaga aaaccgaaaa
    11401 ccaaaccaaa ggaacacaaa aagtggaaca acaagcggtc gaaaaacaag aaaatattgc
    11461 agttgagcca atcagccctg aaattacgat tgatgatttc gcgaaattag atttacgtgc
    11521 agccaaagta ttgaactgcg aggctgtgcc aaaatcagac aaaatgttac gttttgagct
    11581 ggatttaggc gaccaccaac gccaagtatt ctcaggcatt aaggcggcct acccaaatcc
    11641 ggaagcactt atcgggcgtt ttgtgattgt aattgccaac cttgctccac gcaaaatgtc
    11701 gttcggtatg tcggagggaa tgattttatc tgccggcaca ggcggtgcga acctgttctt
    11761 attagatgca gacagcggtg ttcagccggg aatgcaagtt aaataggaga agatgatgat
    11821 tcaacaaacg ctttctatta ttaagccgga tgctacacgc cgtaatttaa tcggcaaaat
    11881 tttggctcaa ttggaagaaa acgggctgac tatcaaagct ctaaaaaaag tacatcttac
    11941 ccgtgagcaa gcggaaggat tttatgcgga acacaaaggc aaagatttct ttgagccgtt
    12001 agtggaattt atgacatcag gatctattgt cgtttctgta ctggaagggg aaaatgcaat
    12061 tagccattat cgtgcattaa tgggggcaac caatccggat aaccgtgaaa aaggcaccat
    12121 tcgcgattta tatgccttaa gtatgcgtga aaattcggta cacggttcag attccgaaga
    12181 gtcggcaaaa cgtgaaattg cctacttctt tgtaccgagt gagattgttt aataaagtag
    12241 gggcgaaaat tttttcgccc cactattgta attcgcccta gcgtagatta aatatattct
    12301 acgccaacaa tgtcaaactc aactttgcca cccggtgtgg taatgctgac tgaatcgtcc
    12361 acctctttgc cgattaaacc acgagcaatc ggggaattaa ctgaaattaa gccctcttta
    12421 atattggctt catcatcacc cacaatgcgg taagtgactt cttcatctgt atcagcattc
    12481 actaactgca ccgttgcccc aaaaatcact ttgccgttgt ttgccatttt agtgacgtca
    12541 ataatttgag cattgcctaa tttgccttca atttcttgaa tacgcccctc acagaaacct
    12601 tgttgttcac gggctgcgtg atactccgcg ttctctttta aatcaccgtg ttcacgggct
    12661 tctgcaatcg catcaataat ttgtgggcga cgcacatttt ttaaaaaatc caattcttca
    12721 cgcagttgct ctgcaccacg caccgtcata ggaatttgtt tcattaaatc tatttcctta
    12781 ctaaaaatta tcggtttacg aattaaaacg aaagggctat tattcaattt ctcactaaaa
    12841 atttcaactc cccttttatt atgattttta aaaatttcac tcgtgctttt ttaaataaat
    12901 ttatcttatc tacacttctg attccaagca ttacacaggc tgaaattaat accgaagaat
    12961 tattaaatac cttgcctgcc ggtacttctg ttagttttat tgctaaaaat ttagacacta
    13021 accaaatcat cacccaatac caaagtgata tttttatgct acctgccagt acccaaaaag
    13081 tctttacggc actggcagca aaattaactt taaacgatga tttccgcttt caaactgccc
    13141 ttttaaccaa cggaaaagta gaaaacggag tgttaaaagg caatttaatt gcccgtttta
    13201 gtggcgaccc tgagcttaca agcggtcaaa tttatcaatt aatgagcaaa ttaaagcaac
    13261 aaggtatcaa taaaattgag ggggatttaa ttctagaccc gtcggttttc gccagccacg
    13321 acaaagcctc cggttggatt tggaacgact tgacgatgtg ctttaacgcc ccaccggcgg
    13381 caattaatgt ggatcacaac tgtttttatg tcaccttaaa tgccgaccaa cccattggcg
    13441 aatttgctaa agtgaatgtg ccgtccgctt atccggtgca ggtgtttagc tctgcttata
    13501 ttgtcgagcc gaaagaagct ccgttttgcc agcttgatgt ggtggttcac gataacaacc
    13561 gctaccaaat caaaggctgt atggctcgcc agtctcaacc gttcggctta agtttttcgg
    13621 tgcaagaccc aactaattac ggggcaaata tgcttaaagc ccagttaaaa agcctgaaaa
    13681 ttgcctttaa cgggcaagta aaagagccac ttactgccca aaacggcacg ctacttgccg
    13741 agcattattc cgagccgttg ccggtattat tgaaaaaaat gatgaaaaaa tcggataacc
    13801 aaattgccga tgccctgttt agaaccgttg ccaacaagca acacaaccgc cccgcatcgt
    13861 tccaattagg tagttatgtg attcgccagt tattaaaaac caaagccaat attgatttta
    13921 aaaacagcgt ggtggctgat ggctcagggc tttcccgtca taatcaagtc agcagccgaa
    13981 caatgctgga gacccttgaa tatatcgctc aaaacgaaga aagcttaaaa ctgtttgaaa
    14041 ccttcccgat tgcaggcgtg gacggtacta tttccggcag aggctctatt tcaacagaac
    14101 cgcttgccaa aaatctgatt gccaaaaccg gctcactgaa aggggtttac aaccttgccg
    14161 gctttatgaa aaacgcccgt ggcgaacgca tcgcttttgt gcaatttatt aacggctatt
    14221 ccaccggcga gctcgaaagc aaaaccaaac gggctccgct taatacattt gaaaataagt
    14281 tttatatggc gttgtttaat gaatagggct tatattttgt gtgatggatt tttgagaatt
    14341 gaataagaca gcggtcgtaa atttttgaaa ttttacgacc gcttgctcct ctctaagcaa
    14401 ttaaatctcg atgacttcgt tgcattttta cctcaatata actacaactt ggcttgattt
    14461 tcagcccttt ttcttgcgca aacgcaatta aagcattgta aagctctcct gcaatacctt
    14521 taccttgaaa ggcatcatct actcgggtac tgtaagcatc aatcacgttc tcttttatgt
    14581 agcgataacg tagtgtgcct gcttttaggt tgttttcggt aaaatactca aatacgaaat
    14641 ctgctaaatt gtggttaatc atagttcctc cgattttttt cctctattct acccgcttta
    14701 atcacgagtg caagcggtcg attttgcaga aaaatttgca aaatcgacca cttgtagatg
    14761 aaaaaagcaa acgtttgcgt tataatcttt gccgtttatt ttcacgttaa cactcatttt
    14821 aaggaattta agaatggcga ttcaacaggc gttattaagt gtgtctgata aaaaaggcat
    14881 tgtcgaattt gctcgtgggc tatcggctcg tggggtaaaa ttactttcta cgggcggaac
    14941 cgcaaaatta ctggctgaag ccggtttgct agtaacagaa gtatcagatt acaccggctt
    15001 tcctgaaatg atggacgggc gtgtaaaaac cctgcacccg aaagtgcacg gcggaattct
    15061 aggtcgccgt ggtactgatg atgaagtgat gaaccaacac ggcattgaac gaattgatat
    15121 ggtggtagta aacctctacc cttttgctca aaccgttgcc aatccaaatt gtagccttga
    15181 agatgcggtg gaaaatattg atatcggtgg tccgacaatg gtgcgttctg cagcgaaaaa
    15241 tcataaagat gtggcgattg tggtcaataa caatgatttt gatgctattc ttgccgaaat
    15301 ggatcaacat caaaacagtt taactttaga aacccgtttt aatcttgcta ttaaagcgtt
    15361 tgagcatacc gcacaatacg attcaatgat tgccaactat ttcgggcaaa aagtgccgcc
    15421 ttatcaaggg gctgaggaag aagatttaac ccaaccaagt ggacaattcc ctcgcacctt
    15481 aaacctcaat tttgtgcgta aacaaacgat gcgttatggc gaaaattcgc atcaaaacgc
    15541 tgcattctat gtcgaaaatg agttaaaaga agcctctgtt tccaccgcca aacagttaca
    15601 aggtaaagcc ctttcgtata ataatattgc agataccgat gcggctcttg aatgcgtgaa
    15661 agaatttagc gagcctgctt gtgtgattgt aaaacacgct aacccttgtg gtgttgcttt
    15721 gggtaaaaat attttagatg cctacaaccg tgcttaccaa accgatccaa cttctgcttt
    15781 cggtggcatt attgccttta accgtgaatt agacggcgaa actgctcaaa ccatcgcaga
    15841 ccgtcaattt gttgaagtga ttattgcacc aaccgtttct gcttctgcca aagaagtgtt
    15901 agccactaag aaaaacgtgc gtgtgcttga atgtggcgag ttctcagaag ctgtaaaacg
    15961 cttagatttc aaacgggtaa acggcggatt attagtgcaa gatgccgacc aaggctctgt
    16021 atctattgac gatttaaagg tggtttcaga gcgtaaacca actgaagctg agctaaaaga
    16081 cctattgttc tgctggaaag tggcgaaata tgtgaaatcg aatgcgattg tgtatgccaa
    16141 agacgggcaa actatcggta tcggtgcagg gcagatgagc cgtgtttatt ccgcaaaaat
    16201 tgcaggtatt aaagcggaag acgagggctt acaagttgtg ggttgtgtaa tggcttccga
    16261 tgcgttcttc ccgttccgtg atggtattga cgctgctgca aaagtaggga ttacttgtgt
    16321 gattcaccca ggtggttcaa tgcgtgatca agaagtgatt gatgccgcca acgaacacgg
    16381 tatggcagtg gtactaaccg gaatgaggca tttccgtcat taatgttcta caaactaaaa
    16441 ggctacctta ttttgataag gtagcctttt tcattaatta atcacaaggt aaaattttga
    16501 ttgctaagcc accggttgag gtttcacggt attttgcatt catatctttg ccggtttcgt
    16561 acattgtttc aatgacttta tctaaactta cctgaggcga agaagtacgg cgtagtgcca
    16621 ttctgctggc attaatcgct ttgactgaag cgattgcatt tctttcaata caaggcactt
    16681 gcacttgtcc gccaacaggg tcacaagtta ggcctaaatt gtgctccatt gcaatttctg
    16741 cagccataca cacctgttca gcactgccac ccataatttc cgttaaacct gctgccgcca
    16801 ttgaacaagc aacaccaact tccccttggc aacctacttc agcaccggaa atagaggcat
    16861 tcattttgta taacgaacca atcacactgg tagtcaataa ataacgctca atagtatctt
    16921 gattaagctg tgcaataaat ttttggtagt acgccaacac agccggaaca ataccacagg
    16981 ctccattggt aggtgcggtg actactcgcc cacctgccgc attttcttca ttcacggcaa
    17041 gggcaaacat attcacccaa tcgataattt gcattgggtc ggtattgaat ttactgtttg
    17101 cctctaacat tctgcgtagc ccactggcac ggcgtaccac tttcagaggc ccgggtagta
    17161 acccttccgt attcaaacca cgctcaatac aggcttccat tgtattccaa atcattgcca
    17221 aataatccga aatttctact ctactgcgta aagcaagctc atttttccag actaaagaag
    17281 aaagcggtaa cccgttattc ttacaatgtg caagtaaatc tgctgcattc tgatatggga
    17341 atggaacaga aagatctgaa tttttaactt gctcaaagtt agcttcatca acaataaagc
    17401 caccaccaat agaataatag gtgttttgaa atagcaactc ttcaccgctc aaggcactaa
    17461 atttcacacc attttcgtgc cgaggtaaga aatctcgatg aaatggcata tcctcataaa
    17521 aatcgaacgc aatcacttta ggctcaacac tgctttcagc aatgataagt tgcttatttt
    17581 gttttagatt ttcaatcgtc tgctcaatac gttcaatatc cacattatga ggcaaatacc
    17641 ccattaatcc cattatgatg gcaatatccg tattatgtcc tcgcccggtg agtgataatg
    17701 aaccatagac atctacccgc aagcggtcaa ttttgtttaa aaatttgcga ttttgtaatt
    17761 catcgacaaa ttcctttgcc gctttcatcg gccccgcagt atgggaactg gaaggcccaa
    17821 tccctacttt aaacatatca aatacactaa tcataaacac aaattcacat agaaaataaa
    17881 agggcgtatt ctacattgat tttagaaaga ttacagtaac ccatatacta ttgctgaaat
    17941 agcaatacaa cccataacaa gcacaaataa atggccaaat tcacctttaa aacgtgcaag
    18001 tgccggtact ttatatacgg catagacagg cataataaac aagatcatcg caataatcgg
    18061 cccaccaagg ctttcaatga agcctaaaat gcttggattg atagtcgcaa caatccataa
    18121 aatgatcaaa aaagtaatag cggtaatttt gcggaatttg cttggatcaa tcggctcttt
    18181 acgcatttga ttaaccaatc cttctaaccc ctcgcgcgca cctaaataat gcccaaagaa
    18241 agagctacca atcgccaaaa aagcaaccaa cggtccaaaa taggaaataa tcggattatc
    18301 aaatttattc gcaaggtaag aaagaatcga aatattttgc actttagctt gtgctaattc
    18361 ttcaggtgtc aaagttaata cacagctaaa tacaaagaac atcacaaata acaccaagat
    18421 aaaggcggta gaacgtaata ctttagctga ttcaatttca gccttcttgt catcttggta
    18481 atatttctgt tgagaaagtg caaaagaaga aatagccggt gaatggttaa aagcaaagac
    18541 taaaacagga atagtcagcc aaagtgtagt aaggaagtct cctgtagttg gcatttgctg
    18601 taaggcagct gtattccact gtggaattaa ataaatagat aagcccaata aaatcaacac
    18661 taatggatac acaagacaag ttgtgatttt taacatgact tgctcactta ataacattac
    18721 tccgatcata gcagctatta gcataaacga taatacagcc ctagatggtg gcgcgatatg
    18781 aagctgattc tccataaaag aactcacggt attggtaata cctacaccat aaatcagtaa
    18841 aatcggaaaa atggcaaaga agtacagtag cgtgatcaat ttaccggcct gttcaccaaa
    18901 atgttcacga acaacagccg taatatcgct ccctttgtat tttgaagaaa gcacaaaacg
    18961 tgttaagcca cggtgagcta aatatgtcat tggtccaact aacaaagtca taataataag
    19021 tgggtagaac ccacccatac ctgcattaat tggcaaaaat aaaacgccgg ctcctaccgc
    19081 agtaccaaat aaatttagta cccaagtaag agaaaaacgt tgagatggca tagccatagt
    19141 aatctccttt aaagaattat ctaacaagct atgctatatt agcgatggga aacagtaaca
    19201 caacaagatt
//
LOCUS       DANLYI010000027        18923 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.027, whole genome shotgun sequence.
ACCESSION   DANLYI010000027 DANLYI010000000
VERSION     DANLYI010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 18923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18923
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.027"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            14..2449
                     /gene="plsB"
                     /locus_tag="PXD81_000714"
     CDS             14..2449
                     /gene="plsB"
                     /locus_tag="PXD81_000714"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HDL5536767.1"
                     /translation="MSGLLNFYRAFLNLPLSLLVKSRSIPTHPVAELELNLEQPIVYV
                     LPYTSQTDLLILQKNCLALNLPDPLQENVIEGQTLLRFVFLDEGRRFFKSKGAKSETE
                     SIFYRYLDLHRANAELDVQLVPVSVLWGRAPGKEDARHLQVLTSFQRFLSMVWFGRDN
                     FVRFSPAVSLRYMVTEYGADEKIAQKIARVAKMHFAKLRYSAMGPRLPNRDAMFNKIL
                     NSEVIQAAIAEEAKKSSPEKARKEAEKIINEIAADVKHESLRVADRVLSWLWNKLYQG
                     INVQNADRVRKLALEGHEIVYVPCHRSHMDYLLLSYLLYHQGLVPPHIAAGINLNFWP
                     AGPIFRSWGAFFIRRTFKGNRLYSTIFREYLAELFYRGYSVEYFIEGGRSRTGRLLEP
                     KTGMMSMSLQALQRGLNRSLSIVPVYIGYEHVLEVDTYAKELRGAAKEKENAGLVLRV
                     LKKLKNLGQGYVNFGEPILVNNYLNQYFPEWKEPSEDGRPKWLNEAVDNISHQVMVNI
                     NKAAAVNAKNLVGSALLASRQRALTREQLIEQLGSYIQLFRNVPYSKDMTLPTESAEV
                     MLNHVIHLPRSGVLIEKDNFGELVRLERESAVLMTYYRNNVQHLFVLPSLVASMVLHH
                     EAVSKDVVIKSVNRIYPFLQAELFLHFKQEELKAHIEAIIAELSRQGVIKNESDMLMI
                     NRSHIRTLQLHSAGVREILQRYYISLSVLLEQPNISRGYLEKESSSIAQRLSVLHGIN
                     APEFFDKAIFSTFTSSLKAQGYFDGDDNLAIAKVKETESLLRSLISIEVQQTIQGAMA
                     KVAESVENPNIAG"
     gene            complement(2554..3117)
                     /locus_tag="PXD81_000715"
     CDS             complement(2554..3117)
                     /locus_tag="PXD81_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005613534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HDL5536768.1"
                     /translation="MSAPNSGTNPAPLGLCGFALTTFLLSLINNGTFSGENVGLVLAM
                     GFAFGGTSQMIAGMFEFAKGNTFGFTAFTSYGAFWWSFALFKVFFAANVSAEFVGWYL
                     VAWGTFTLMMFIGTLAKARALQAIFLSLTITFYLLAAGDFTHNTTLTNIGGNFGLLTA
                     FLAFYLAAADIINESFGKTVLPIGEPK"
     gene            complement(3315..4211)
                     /locus_tag="PXD81_000716"
     CDS             complement(3315..4211)
                     /locus_tag="PXD81_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL5536769.1"
                     /translation="MDILDRLLAFAQISAGISVKCQLQGDWQLENPHQTGQAIAHIIS
                     YGQAYLQHNGKTHHLKQGDIVFFPRFADHTLRSQIQPLTSKNAVNPEQLGAFTLTQAG
                     LSQSECDMFCLHFYYDHQAELMESLPEMIVFQVAETPLKAMLDILKQEAETPNLAVKS
                     VVNSLAVVILTMILREHLVNHNNQLFGILKGWQDLHLRPLIKAILLNPEHEWQVDDMA
                     AFIHLSRAQLVRLFNKHLCISPHAFVHKIRLQKAAMLLKSQAGSVLSIALSCGFQSES
                     HFITAFKKQYCVTPSQYRQLNP"
     gene            4347..4688
                     /locus_tag="PXD81_000717"
     CDS             4347..4688
                     /locus_tag="PXD81_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HDL5536770.1"
                     /translation="MSSFQNWTEHTAHVKQSFGKLGQKHPKMLEAYKALGTAAAAEAL
                     DHKTRELIALAVAITTRCESCISVHAEEAAKAGATESEIAGALATAIALNAGAAYTYA
                     LRALEAVEEQK"
     gene            4699..5052
                     /locus_tag="PXD81_000718"
     CDS             4699..5052
                     /locus_tag="PXD81_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HDL5536771.1"
                     /translation="MKKLAWILGFAAMMPMMSSAKQASVQQVQAEKAAGIWIDVRSAE
                     EFQQGHLNGAINVTHTEIAQRIAEVAPDKNQPIHLYCRSGHRAEAALNELKKLGYSNV
                     TNHGGYDDLIKAGLK"
     gene            5112..5750
                     /locus_tag="PXD81_000719"
     CDS             5112..5750
                     /locus_tag="PXD81_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005719747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgB family protein"
                     /protein_id="HDL5536772.1"
                     /translation="MSAFNNFVNFVANIVAPMQRQFINFVRIAICIVMVWIGGLKVCQ
                     YEADGIAHFVSNSPFFSYMYKKGPNLVDDGTGKMVMEYTLHKNPEGKMVAKNIEWHKE
                     NGTYTASYIIGATIVTVGLLTLSGIWFPVSGMAGGLLTFGMSIVTLSFMITTPEIWVP
                     NLGGDMPTPAHGFPYLSAVGRLIVKDVIMMAGGLVAAAECANRILARRKAIA"
     gene            5885..7066
                     /gene="purT"
                     /locus_tag="PXD81_000720"
     CDS             5885..7066
                     /gene="purT"
                     /locus_tag="PXD81_000720"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005615456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HDL5536773.1"
                     /translation="MTTIGTPLRTNATKVMMLGSGELGKEVVIELQRLGVEVIAVDRY
                     DNAPAQQVAHRAYTISMLDGNALRDLVEKEKPDFIVPEVEAIATATLVELEQEGYNVI
                     PTAKATQLTMNREGIRRLAAEELGLKTSPYRFVDNFEQFQQAIQEIGIPCVVKPIMSS
                     SGHGQSVIKSEADIQQAWDYSQQGGRAGGGRVIVEGFIKFDYEITQLTVRHIHGTSFL
                     APIGHIQVDGDYRESWQPQQMSDIALKKAQETAEKITSALGGRGIFGVELFVCGDEII
                     FNEVSPRPHDTGIVTMASQELSQFALHARAILGLPIPEIYRISPAASKAIVVEGKSDN
                     VRFGGVDKVLAEIGTNIRLFGKGEVNGHRRLGVILARDENTVRALEKARRAYDKLDIQ
                     L"
     gene            complement(7158..8651)
                     /locus_tag="PXD81_000721"
     CDS             complement(7158..8651)
                     /locus_tag="PXD81_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005556599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDL5536774.1"
                     /translation="MFKQLQQVGKAFMLPIAILPAAGLLLGIGGALSNKATVQAYPIL
                     DNPTLQGIFQIMASAGSVVFGNLALLLCIGLGIGLAKRDKGVAALAAVVGYLIMTGTI
                     SALIPLFPPETKGIDTGVIGSLVMGLITVKLHNKYHNIQLPQILGFFGGSRFVPIVTA
                     FAAIFVGAVFFLIWPAFQQWLVSAGQGIAEMGEIGTFFYGFLMRLSGAVGLHHMIYPL
                     FWYTELGGTEVVNGETIIGAQKIFFAQLADPTHQGLFTEGTRFFAGRFDTMMFGLPAA
                     CLAMYHAVPKARRGAISGLFLGAALTSFITGITEPIEFMFLFVAPWLYVFHAFLDGVS
                     FYIADLLNISIGNTFSGGVIDFTLFGILQGNENTNWLRVIIVGLPWAALYYFSFLFLI
                     KKFNVMTPGRAEESEEVVEQQTSSLTENAHKIIAALGSANNIENVDACITRLRVAVKE
                     VKAVDKATLKKLGAIDVLEVGGGVQAVFGAKAVLYKSEVNQILGKED"
     gene            8852..10699
                     /gene="ilvD"
                     /locus_tag="PXD81_000722"
     CDS             8852..10699
                     /gene="ilvD"
                     /locus_tag="PXD81_000722"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012055207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HDL5536775.1"
                     /translation="MPILRSATSTQGRNMAGARALWRATGMKENDFGKPIIAVVNSFT
                     QFVPGHVHLKDMGQLVAAEIEKAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRDLIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMAAMRLNIPTIFVSGGPMEAGKTKLSDQLI
                     RLDLVDAMIESADPNVSDERIDAIERSACPTCGSCSGMFTANSMNCLTEALGLSLPGN
                     GSMLATHADRKELFLRAGREIVELCRKYYQQDDESVLPRSIATFDAFENAMSLDIAMG
                     GSSNTVLHLLATAQEAGVDFKMEDIDRLSRVVPCLSKIAPNTNKYHMEDVHRAGGIMG
                     LLGELDRAGLIHRHVKTVLGLTIEEQLNQYDIIRNKDEDLHKFFRAGPAGIRTTKAFS
                     QDCRWDTVDDDRVNGCIRNVENAVTKEGGLAVLFGNIAEDGCIVKTAGVDESIWKFTG
                     TAIVFESQEDAVAGILGGKVKEGHIVIIRYEGPKGGPGMQEMLYPTSYLKSMGLGKKC
                     ALLTDGRFSGGTSGLSIGHASPEAASGGAIALINDGDTVAIDIPNRSINLMVSDEELA
                     ARRAEMEARGDKAWQPLKREREVSFALKVFGHFATSADKGAVRDKSLLK"
     gene            complement(10833..11183)
                     /locus_tag="PXD81_000723"
     CDS             complement(10833..11183)
                     /locus_tag="PXD81_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TusE/DsrC/DsvC family sulfur relay protein"
                     /protein_id="HDL5536776.1"
                     /translation="MWHFYLMTIELNNIQYPTDASGYLTNLNDWSVELAEKIAEKEQI
                     SLTAEHWEIIYLVRDFYQEYKTSPAIRMLVKTMAQKFGEEKGNSRYLQRLFPDGPAKQ
                     ATKIAGLPKPAKCL"
     gene            complement(11212..11877)
                     /locus_tag="PXD81_000724"
     CDS             complement(11212..11877)
                     /locus_tag="PXD81_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011848341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BAX inhibitor protein"
                     /protein_id="HDL5536777.1"
                     /translation="MENRLAHSYSTGESLISTHKVLRNTYMLLAMTLTFSAVVAFVAI
                     QMNAPAMPWWGLLVGFYGLLFLTNATSNSSAGILSVFALTGFLGYTLGPILNRYIGAG
                     LGDIVALAFGATALVFFACSAYVLTTKKDMSFISGMMVALFVVLLVGIIANIFLAIPA
                     LSLALSSLFVIFSSGAILLATSNIIHGGETNYIRATVDLYVSIYNLFVSFLQIFGVLG
                     SDD"
     gene            12083..12631
                     /gene="pgsA"
                     /locus_tag="PXD81_000725"
     CDS             12083..12631
                     /gene="pgsA"
                     /locus_tag="PXD81_000725"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HDL5536778.1"
                     /translation="MKLNFPTYLTLFRVGLTPLFIVAFYLPTKYAPEISTLIFFIASI
                     TDAFDGYLARKWNQTTRLGAFLDPVADKVLVAVAFVCVAEYYHTWWITIPICIMIARE
                     IIISALREWMAELGERASVAVSIWGKVKTTAQMLALGGMLWRQNQLMEILAFILLYIA
                     VGLTIWSMMQYLNASKNSLLKS"
     gene            12709..14547
                     /gene="uvrC"
                     /locus_tag="PXD81_000726"
     CDS             12709..14547
                     /gene="uvrC"
                     /locus_tag="PXD81_000726"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HDL5536779.1"
                     /translation="MSEFNAKSFLLNVPHQPGVYRMYDSKGTIIYVGKAKDLKKRLAS
                     YFRSQLSSKKTEALVANLANIETTITHSETEALLLEHNYIKENQPKYNVLLRDDKSYP
                     YILLTKHKHPRLTAFRGSKKIAGEYFGPYPNVTAVRETLNLLQKIFPIRQCEDTYYKN
                     RSRPCLQYQIGRCLAPCVEGCYSPVEYDQQVNLVRLFLQGKDHQVVEHLVQKMEDAAE
                     ALDFEGAARFRDQIQSVRAVQEKQFVSNDRLDDLDIISIVYQNGIACVHILFVRQGKV
                     LGNRSYFPKVPSNTDLTELADTFVGQFYLQMNQHRTIPNQIVIDHQLSESKALSEILS
                     EQAGHKVSILDNAIRGDKQRYLALARTNAMAALVLQLKQESHIQSRYQALKEMLKMSE
                     IKRMECFDISHTMGNQTVASCVVFDENGPLKSDYRRFNIEGITGGDDYAAMEQALLKR
                     YNRELPPEKIPDIIFIDGGKGQLNRALDVFAKLDVEWDKSKPLLIGVAKGVERKAGLE
                     TLIISKWDKEIHLPPDSLALHLIQHIRDESHQHAISGHRKKRQKAFTESGLESIAGVG
                     AKRRQALLKYLGGMQGVKSATLEEIQSVPGISKALAEVIFDTLKNS"
     gene            complement(14552..14827)
                     /locus_tag="PXD81_000727"
     CDS             complement(14552..14827)
                     /locus_tag="PXD81_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5536780.1"
                     /translation="MKKLILLGLSVALTACSTPQQQSTVPMDMQAVQDYHQRVINKQT
                     VDKNTKQHDEPLNQSDKRSESINLPIHPIIYPSIGVGYHYGWKRYRY"
     gene            complement(14952..15305)
                     /gene="rplT"
                     /locus_tag="PXD81_000728"
     CDS             complement(14952..15305)
                     /gene="rplT"
                     /locus_tag="PXD81_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009635878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HDL5536781.1"
                     /translation="MARVKRGVIARARHKKVLKAAKGYYGARSRVYRVAFQAVVKAAQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGLSYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFAALVEKAKSAL"
     gene            complement(15414..15611)
                     /gene="rpmI"
                     /locus_tag="PXD81_000729"
     CDS             complement(15414..15611)
                     /gene="rpmI"
                     /locus_tag="PXD81_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HDL5536782.1"
                     /translation="MPKIKTVRGAAKRFKKTASGGFKRKQSHLRHILTKKTTKRKRQL
                     RHKSMVAKADQVLVVACLPYA"
     gene            complement(15875..16417)
                     /gene="infC"
                     /locus_tag="PXD81_000730"
     CDS             complement(15875..16417)
                     /gene="infC"
                     /locus_tag="PXD81_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015871208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HDL5536783.1"
                     /translation="MKPVKRVQSNRAHRLNDEIRGVKEVRLTDENGEQVGIVSLQDAL
                     NRAEEAELDLVEISPNAEPPVCRIMNYGKFIYEKEKAAKEQKKKQKVVQVKEIKFRPG
                     TDDGDYQVKLRSIIRFLEDGDKVKITVRFRGREMAHQEIGMDVLERVKADTAEIAMVE
                     SAPGKLEGRQVVMVIAPKKK"
     gene            complement(16576..18375)
                     /gene="recQ"
                     /locus_tag="PXD81_000731"
     CDS             complement(16576..18375)
                     /gene="recQ"
                     /locus_tag="PXD81_000731"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HDL5536784.1"
                     /translation="MNTTAISVLNNIFGYQKFRDGQEEVINAVLENRDCLVIMTTGGG
                     KSLCYQVPALCLDGITLVISPLISLMKDQVDQLITNGIEAAYLNSTQTLEEQQLVEQK
                     ALSGQLKLLYLSPEKVMTQGFFHFISLCKISFIAVDEAHCVSQWGHDFRPEYTLLGGL
                     RNTFPNVPLMALTATADLTTRSDILHHLRLNSPHIYLGSFDRPNIRYTVQEKFKPLEQ
                     LIKFISKQQGKSGIVYCNSRKKVEEITEKLSARKISVMGYHAGMSFEQRERVQNAFQR
                     DDIQIVVATIAFGMGINKSNVRFVVHFDLPRSIESYYQETGRAGRDDLPSEAVLFYDP
                     SDYAWLQKILLEEPESEQRNIKQHKLQAIGDFAESQTCRRLVLLNYFGEHRQEQCKNC
                     DICLDPPQQYDGLLDAQKVMSVIYRTGQIFGQHHIIAVLRGMNNLKIREHQHDQLSVY
                     GIGKEQSQHYWQNVVRQLIHLGLIIQNIANHSALQLTESARPFLRGEKQLQLALPRLG
                     ISATAFVQKQASHRYDKDLFARLRFLRKQIADLENIPPYIVFNDATLQEMAEFLPTSK
                     VEMLEINGVGERKFERFGQAFMSLIKEYQNNRK"
     gene            complement(18484..18789)
                     /gene="cyaY"
                     /locus_tag="PXD81_000732"
     CDS             complement(18484..18789)
                     /gene="cyaY"
                     /locus_tag="PXD81_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005612534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HDL5536785.1"
                     /translation="MNTAQFHQAVEQVWQQIEEKIYDEGLSVDTEIQGAVCTLTFDDE
                     SQVIINKQEAMLELWLASKLGGFHFKNVEGEWVTDEGRSFWQHLEEAFARHGESVSF"
BASE COUNT         5489 a         3600 c         4144 g         5690 t
ORIGIN      
        1 aaaggatatt tatatgtctg gcttattaaa tttttatcga gctttcctaa acttaccact
       61 ttcactttta gtaaaatctc gctctattcc gactcatccg gtggctgaac tggaactaaa
      121 tcttgaacag ccgattgtct atgttctacc ttatacctcg caaaccgatt tattaatttt
      181 acaaaaaaac tgcttggctt tgaatttgcc cgatccgttg caagaaaatg tgattgaagg
      241 gcaaacattg cttcgttttg tatttttaga tgaaggtcgc cgttttttta aatctaaagg
      301 ggcgaaaagt gagacagagt cgatttttta tcgttattta gatttacatc gtgctaatgc
      361 tgaattagac gtccaattag tgccggtttc ggtattatgg gggcgggctc cgggcaaaga
      421 agatgcccgc catttacagg tgcttacctc attccaacgt tttttatcca tggtttggtt
      481 tgggcgtgat aattttgtac gtttctcccc tgcagtatcg ttacgctata tggtaactga
      541 atatggtgca gatgaaaaaa tcgctcaaaa aatcgcgcgt gtggcgaaaa tgcactttgc
      601 aaaattgcgt tattcagcaa tggggcctcg tttaccaaat cgtgatgcga tgtttaataa
      661 aatcctgaat tcagaggtga ttcaagctgc gattgcggaa gaagcgaaga aatcatcgcc
      721 tgaaaaggct cgcaaagaag ccgagaaaat cattaatgaa attgccgctg atgtaaaaca
      781 cgagagtttg cgtgtagcag atcgagttct gagctggctt tggaataagc tttatcaagg
      841 gatcaacgtg caaaatgcag accgtgtgcg taaattggca ttagaaggtc acgagatcgt
      901 ttatgtgcct tgccaccgta gccatatgga ctatttgctc ctatcttatc tactttacca
      961 tcaaggatta gtaccgccac atattgccgc tgggatcaat ttaaatttct ggccggcagg
     1021 cccgattttc cgtagttggg gagcattttt cattcgccgt acctttaaag gaaatcgcct
     1081 ctattccacc attttccgtg aatatttagc ggaattattc tatcgtggtt attcggtcga
     1141 atattttatt gaaggcgggc gttcacgcac aggtcgttta ttagagccta aaaccggtat
     1201 gatgtcgatg agcctacaag ccttacagcg agggctgaat agatctttaa gtattgtgcc
     1261 tgtttatatc ggttatgaac acgtcttaga agtcgatact tacgctaaag agttgagagg
     1321 tgcggcaaaa gagaaagaaa atgcgggctt ggtgctacga gtgctgaaaa aactgaaaaa
     1381 tttaggtcaa ggatatgtga atttcggtga gccaattttg gtgaataatt acttaaatca
     1441 atatttccct gagtggaaag agccttccga agatggacga cctaagtggt taaatgaagc
     1501 cgtggataat atttcacatc aagtaatggt gaatatcaat aaagcggcag cagttaatgc
     1561 gaaaaactta gtggggtcag cattattggc atctcgccag cgtgcattaa cccgtgaaca
     1621 actcattgaa caacttggaa gttatattca gttatttcgt aatgtacctt attctaaaga
     1681 tatgacatta ccaactgaaa gtgcagaagt gatgttgaat catgttattc atttacctcg
     1741 ttcgggcgtt ttaattgaaa aagataattt tggtgaactg gttcgtttag aacgagaatc
     1801 tgctgtgtta atgacatatt acagaaataa tgtgcagcat ttatttgtgt tgccttcact
     1861 tgttgcaagt atggtacttc atcatgaggc tgtttcgaaa gatgttgtga ttaagtcggt
     1921 taatcgtatt tatccattct tgcaagcaga gttattctta cactttaagc aagaagaatt
     1981 aaaagcacat attgaagcaa ttattgcgga actaagtcgg cagggggtga ttaaaaacga
     2041 aagtgatatg ttaatgatca atcgctcaca tattcgtacc ttgcaattac attcagctgg
     2101 tgtgcgtgaa atccttcaac gttattatat tagcctaagt gtcttgttgg agcagccaaa
     2161 tattagtcgt ggctatttag aaaaagagag tagttcaatt gctcagcgtt tatcagtctt
     2221 gcatggtatt aatgcgccgg agttttttga taaggcgatt ttctctacct ttacatcaag
     2281 tctaaaagca caagggtatt ttgatggtga tgataattta gcgattgcaa aagttaaaga
     2341 aacggaatcg ttgttacgta gtttgatttc gattgaagtt caacagacca ttcaaggtgc
     2401 gatggctaaa gtcgcagaat ctgtagagaa tcctaatatc gcaggataat aaatcaaaga
     2461 agggcgctaa caagcgctcg tttcttgcaa ttttgttgcc aaaaataaaa gctgaatgcg
     2521 cgaccacatt cagctttttt acttcctata taattatttt ggttcaccga ttggtaaaac
     2581 agtcttacca aaactttcat taataatatc cgcagcggct aagtagaacg ctaagaatgc
     2641 ggttaataaa ccgaagttac caccaatgtt agttagggtg gtgttatggg taaaatcacc
     2701 tgcggctaaa aggtagaaag taatggttaa acttaagaaa atagcttgta atgcacgggc
     2761 ttttgctagt gtgccgatga acatcataag tgtgaatgtt ccccaagcta ctaagtacca
     2821 accaacgaat tctgccgata cattcgcagc aaagaacact ttaaataatg caaaagacca
     2881 ccagaatgca ccatagctgg taaacgcagt aaagccgaac gtgttacctt tagcaaattc
     2941 aaacatacct gcaatcattt gtgatgtgcc accgaaagca aatcccattg ctaaaactaa
     3001 acctacgttt tcaccgctga atgtaccgtt attgattagg cttagtaaaa atgtagtaag
     3061 tgcaaaaccg cataaaccga gcggagccgg gttagttcct gaatttggag ctgacattga
     3121 aaacctctgt tatattgtta caccataagt gtggtaattg tttatttacg ccgataaatc
     3181 agacgttatg tgcctaaaat ttagggcgat aaagtatagc gattttgcag taaattgact
     3241 agaaatttcc gagacaagcg gtgagatttt tgcaaaattt tgccaaaaaa tgaccgcttg
     3301 ttgttgtcat ttagctatgg atttaactgc cgatattggc tgggtgttac gcaatattgc
     3361 tttttaaagg cggtaataaa atgcgactcg gattggaagc cgcaagataa ggcgatactt
     3421 aataccgacc cagcttggct tttgagcagc attgctgctt tttgtagcct aattttgtgt
     3481 acaaaagcat gtgggctaat acataaatgt ttgttaaata agcggactaa ctgggcacga
     3541 gaaaggtgga taaatgccgc catatcatcg acttgccatt cgtgttcggg attaagcaaa
     3601 atagctttga tcaaaggtct taagtgtaag tcttgccaac cttttaaaat accgaataac
     3661 tgattgttat ggttaactag atgttctcgt aagatcatag ttaaaataac aacagcaagc
     3721 gagttaacca cagattttac ggctaaatta ggtgtttctg cttcttgttt tagaatatct
     3781 aacattgctt ttaatggtgt ctcggcaact tgaaatacga tcatttcagg taagctttcc
     3841 attaattcgg cttgatggtc ataataaaaa tggaggcaga acatatcgca ttctgattga
     3901 gaaagtcctg cttgggtgag tgtaaatgca cccaattgtt cagggtttac cgcatttttg
     3961 ctggtcaaag gctgtatttg actacgtaat gtatgatcgg caaatctggg gaaaaataca
     4021 atatctccct gtttaaggtg atgggttttc ccattatgtt gtagataagc ttgcccatag
     4081 ctgatgatat gagcgattgc ttgtccggtt tgatgcggat tctcaagttg ccaatctccc
     4141 tgtaactgac atttcacact gattccggct gagatttgag cgaaagctag aagcctatct
     4201 aatatatcca ttgttcattc cttatatttc caccttaaat tgcgattatt tcattataat
     4261 atgagctgat ttgataataa agtttatcag ataaacgcaa taataaatgc gttttttcat
     4321 attattcact ttaggaggaa atcaatatgt cttcattcca aaattggaca gaacacacag
     4381 ctcacgttaa gcaatctttt ggtaaattag ggcaaaaaca ccctaaaatg cttgaagctt
     4441 ataaagcatt aggtacagcg gccgcagcgg aagcattaga tcacaaaact cgtgaattaa
     4501 ttgcattagc agtagcgatt acaacacgtt gtgaaagttg catcagtgta cacgcagaag
     4561 aagcggcaaa agcaggtgca accgaaagcg aaattgccgg tgcattagcg actgcgattg
     4621 cattaaatgc cggtgcggca tatacctatg ctttacgtgc attagaagcg gtggaagagc
     4681 aaaaataagg agcaataaat gaagaaattg gcttggattt taggctttgc agctatgatg
     4741 ccaatgatgt ctagtgctaa acaagcatca gttcagcaag tgcaggctga aaaagcagcg
     4801 ggcatttgga ttgatgtacg ttctgctgaa gaatttcagc aagggcattt aaatggtgca
     4861 attaatgtaa cgcatactga aattgcacaa cgtatcgcag aagttgctcc agataaaaat
     4921 cagccgattc atctttattg ccgctcgggt catcgagctg aagcggcgct taatgagcta
     4981 aaaaaattag gttacagcaa tgtaactaat catggtggtt atgacgatct gattaaagcc
     5041 gggttgaaat aactgttttg agtgccaagc atttgcttgg cacttttcgt ttatatctga
     5101 ggttaaaaat tatgagtgct tttaacaatt ttgttaattt tgttgcaaat attgttgctc
     5161 caatgcaacg ccaattcatt aactttgtac gtattgccat ttgtatcgtg atggtttgga
     5221 ttggtggttt aaaagtctgc caatatgaag cagacggtat tgcccatttt gtgtctaata
     5281 gcccgttctt tagttatatg tacaaaaaag gcccgaattt agttgatgac ggcacaggta
     5341 aaatggtgat ggaatatacc cttcacaaaa accctgaagg taaaatggtg gcaaaaaaca
     5401 tcgaatggca taaagaaaac ggcacttata ctgcatctta tatcatcggt gcaaccattg
     5461 tgacggttgg tttattgacg ttatccggta tttggttccc ggtttccggt atggcggggg
     5521 gcttactcac ctttggtatg tcgattgtta cactctcttt tatgatcacc acccctgaaa
     5581 tttgggtgcc gaatttaggt ggggatatgc caaccccagc gcatggtttc ccatatctgt
     5641 cagcagtcgg tcgtttaatt gtgaaagatg tgattatgat ggcaggcggc ttggttgccg
     5701 cagcggaatg tgcaaatcgc attttagcca gacgtaaagc gattgcttaa ttgatagtgg
     5761 taatgcaagc ggtcattttc ttgtaaaatt ttgcaataaa atgaccgctt gttttttgtc
     5821 aatttgccat aatttcaagt ataattcaat tacgtaatcg tttgctttta tggagaggaa
     5881 taaaatgaca acaatcggta caccgttaag aacgaacgct acaaaagtga tgatgttagg
     5941 ttcgggcgag ttggggaaag aagtggtaat agagctgcaa cgcttaggcg tagaagtgat
     6001 tgcggtggat cgttatgaca atgcacctgc acagcaagtg gctcaccgtg cttatacgat
     6061 ttcgatgtta gacgggaatg cattgcgaga tttggtggag aaagaaaaac cggattttat
     6121 tgtgcccgaa gtagaagcca tcgcaactgc aaccttggtt gagttagagc aagagggata
     6181 taacgtaatc ccaacggcaa aagcaacgca attaacaatg aatcgagaag gcattcgccg
     6241 attggcggca gaagagttgg gcttaaaaac gtcgccttat cgttttgtgg ataattttga
     6301 acagttccag caagcgattc aagaaatcgg cattccgtgt gtggtcaaac cgattatgtc
     6361 ttcttccgga cacgggcaat cagtaattaa atctgaagcg gatattcaac aggcttggga
     6421 ttattcgcaa cagggcggac gtgccggcgg tgggcgtgtg attgtagagg gttttatcaa
     6481 attcgattat gaaattaccc agcttaccgt tcgccatatt cacggcacgt cgtttttagc
     6541 tcccatcggg catattcagg ttgatggcga ttatcgtgaa tcttggcaac cgcagcaaat
     6601 gtcggacatt gcattgaaaa aagctcaaga aaccgcagaa aaaattacct ctgcactggg
     6661 cggaagaggg attttcggcg tggaactttt tgtctgtggc gatgagatta tttttaacga
     6721 agtctcccca cgtccacacg ataccggaat agtaacaatg gcatcacaag agctttctca
     6781 atttgctctg cacgcccgtg cgattttggg cttaccgatt ccggaaattt accgcatcag
     6841 cccggcagct tctaaagcga ttgtggttga agggaaatcg gataatgtgc gttttggcgg
     6901 tgtagataag gtattagccg aaatcgggac gaatatccgc ttattcggta aaggcgaagt
     6961 aaacggacac cgccgattag gggtaattct tgctcgtgat gaaaatacag tgagagcatt
     7021 agaaaaagct cggcgagctt atgataagtt agatattcaa ttataaagtc aggattttga
     7081 tattcgttga tagtaggcat tttatgccta ctatcctata ggcctctaat caaattggat
     7141 gaaaaagttg aagattgtta atcctctttg ccgagaattt ggttcacttc gctcttatac
     7201 aatactgctt ttgctccaaa tacggcttgt acaccgccac cgacttccag cacgtcaatc
     7261 gcaccgagtt ttttcagtgt tgctttatcc acggctttca cctctttcac tgccacacgc
     7321 agacgggtaa tacaagcatc cacattttcg atattatttg cagagccaag agcggcgata
     7381 attttatggg cattttcagt cagtgagctg gtttgttgtt cgactacctc ttcactctct
     7441 tccgcacgtc ccggtgtcat tacgttgaat tttttgatca ggaataggaa cgagaagtag
     7501 taaagtgccg cccacggcaa gccgactatg atcactcgaa gccagttagt gttctcgttg
     7561 ccttgcaaaa tgccaaacag ggtgaagtcg atcacgccac cggagaaggt gttaccaatc
     7621 gagatgttga gtaagtctgc aatgtagaac gacacgccgt ctaaaaaagc gtggaacaca
     7681 tagagccacg gggcaacaaa caggaacata aattcaatcg gctcggtaat gccggtgata
     7741 aaagaggtga gcgctgcacc taagaacaga ccgctaatcg caccacggcg ggctttcggc
     7801 acagcgtgat acatcgctaa acaggcggca ggtaagccaa acatcatcgt atcgaaacgt
     7861 ccagcgaaga aacgtgtacc ttcggtaaat aagccttgat gggttggatc ggcgagttgg
     7921 gcgaagaaga ttttttgcgc cccgataatg gtttcaccat ttaccacttc tgttccgccc
     7981 aattcggtgt accaaaacag cgggtagatc atatggtgta agccgaccgc accgcttaac
     8041 cgcattaaga aaccgtagaa aaaggtgccg atttcgccca tttccgcaat cccttgccct
     8101 gctgaaacca accactgttg gaaagccggc cagatcaaaa agaacactgc cccgacaaaa
     8161 atcgcagcaa aggcggtcac aatcggcaca aaccgagagc cgccaaagaa gccgagaatt
     8221 tgcggcagct gaatgttgtg gtatttgtta tgcagtttca ccgtaatcaa gcccattacc
     8281 agcgagccga tcacaccggt atcaatccct ttggtttcag gaggaaacag cggaatgagg
     8341 gcagaaatcg tgcctgtcat aatcaaataa ccgaccactg ctgccagtgc cgctacgcct
     8401 ttatctcgtt ttgctaatcc aatgcctaaa ccgatacaca gtaataatgc aaggttgcca
     8461 aacaccacac tgcctgccga tgccataatc tggaaaatgc cctgtaaagt cgggttatcc
     8521 agtatcgggt aggcttgcac cgttgcctta ttcgacaaag ctccgccaat ccctaacagc
     8581 aaaccggctg ccggtaaaat cgcaatcggc agcataaaag cttttccgac ttgttgcagt
     8641 tgtttgaaca taaaatttct ccttttttat ggttgtattt gatggtttat agcatactga
     8701 aatttagccg tatgaggtgt gatataactc acagatttgc aaaattttta gcaaattcga
     8761 ccgcttgtaa aaaaattacc gcttgcggaa aaattgaagt agaattaagc atttttttag
     8821 caaacacaac ataaaaaatt tggagaagat tatgcctatt ttacgttcag caacttccac
     8881 tcagggtcgt aatatggcgg gggctcgtgc attatggcgt gcaaccggta tgaaagaaaa
     8941 tgattttggt aagccgatta ttgcagtggt gaactcattc acccaatttg tgccggggca
     9001 cgtccactta aaagatatgg ggcagttggt tgcggcagaa attgaaaaag ccggtggggt
     9061 ggcaaaagaa ttcaacacca ttgcggttga tgacggcatt gcaatgggac acggcggaat
     9121 gctttattct ttaccaagcc gtgatttaat tgcagacagt gtggaatata tggtgaatgc
     9181 tcactgtgct gatgcgatgg tgtgtatctc taactgcgat aaaatcaccc cgggaatgtt
     9241 aatggcagca atgcggttaa atattccgac tatttttgtc tctggtggcc cgatggaagc
     9301 cggaaaaact aaactttccg atcaattaat tcgcttggat ttagtcgatg caatgatcga
     9361 aagtgccgac ccgaatgtgt ctgatgaacg tattgatgcg attgaacgta gtgcttgccc
     9421 gacttgtggt tcttgttcag gtatgttcac cgcaaactca atgaactgtt taactgaagc
     9481 tttaggctta agtttaccgg gcaatggttc aatgttggca actcatgctg accgtaaaga
     9541 gcttttccta agagcaggtc gtgaaattgt ggaactttgc cgtaaatatt accaacaaga
     9601 cgatgagagc gtgttacctc gctcaatcgc taccttcgat gcatttgaaa atgcaatgag
     9661 ccttgatatt gcaatgggtg gttcaagtaa taccgtgctt catttactag cgactgctca
     9721 agaagccggc gttgatttta aaatggaaga tattgaccgc ctctctcgtg tcgtgccgtg
     9781 cttaagtaaa attgccccga ataccaataa ataccatatg gaagacgtac accgtgccgg
     9841 tgggattatg ggcttgctgg gcgaattaga tcgtgcaggt ttaatccatc gccacgttaa
     9901 aacagtgcta ggtttaacga tagaagagca actgaatcaa tacgatatta ttcgtaataa
     9961 agatgaagat ttacacaaat tcttccgtgc aggtccggca ggaattcgta ctactaaggc
    10021 tttctcgcaa gattgccgtt gggacactgt tgatgatgac cgtgtaaacg gctgtattcg
    10081 caatgtggaa aatgctgtga ccaaagaggg cgggttagcg gtattatttg ggaatattgc
    10141 agaagatgga tgtattgtta aaaccgcagg ggtagatgag tctatttgga aattcactgg
    10201 caccgcaatc gtgtttgaaa gccaagaaga tgcggtagca ggtattttag gcggaaaagt
    10261 gaaagaaggg catattgtga ttatccgcta tgaaggtccg aaaggcggcc cgggtatgca
    10321 agaaatgctt tatccaacta gttatttgaa atcaatgggc ttaggcaaaa aatgtgcttt
    10381 attaactgac ggtcgtttct ctggcggtac ctcagggtta tctatcggac acgcttcacc
    10441 ggaagccgct tcaggaggag cgattgcctt aattaatgat ggcgatacgg tggcgattga
    10501 tattccgaat cgttcgatta acttaatggt ttcagatgaa gaacttgctg ctcgccgtgc
    10561 agaaatggaa gcccgtggcg ataaagcgtg gcagccgcta aaacgtgagc gagaagtgtc
    10621 gtttgcacta aaagtgtttg gacattttgc cacctctgct gataaaggtg cggtgaggga
    10681 taaatcgctg ttaaagtaaa taaattagaa aaaagaagca tttcaataat atattgatct
    10741 gctctttatt cagctgaata tttaatctag ctgaatggag agcagatcga tagcctaatt
    10801 tacatctttt tgttttgata cagggaaatg tattacaagc attttgccgg ttttggcaga
    10861 cctgcaattt tagtggcttg ttttgcagga ccatcaggaa acaagcgttg taaataacgg
    10921 ctgttgcctt tttcttcacc gaatttttgt gccattgtct ttaccagcat tcgaattgct
    10981 ggtgaagttt tgtattcttg gtagaaatcc cgcaccagat aaataatttc ccaatgttct
    11041 gcggttaagc taatttgctc tttttcggcg attttttctg ctaattctac agaccaatca
    11101 tttaaattag ttaaatagcc tgaagcatcg gttgggtatt ggatattatt taattcaatc
    11161 gtcatcaaat aaaaatgcca cataatgtgg cattcctctt aaactaaaac gctagtcgtc
    11221 actacctagc acaccgaaga tttgtaagaa gcttacaaat aggttgtaga tagacacata
    11281 taaatcaacg gttgcacgaa tgtagttggt ttcgccaccg tgaataatgt tgctggttgc
    11341 cagtaaaatc gcaccgcttg agaaaatgac aaacaagctg cttagggcta aacttaatgc
    11401 cggaattgct aagaagatat tggcaataat accgactaat aataccacaa acagggcaac
    11461 catcattccg ctgataaagc tcatatcttt tttggtggtt aatacataag ctgaacaagc
    11521 gaagaaaacc aatgcggttg caccgaatgc aagggcgaca atatccccta aacccgcacc
    11581 gatgtaacgg tttaaaatag gacctaaggt atagcctaag aagccggtta aagcaaatac
    11641 gcttaagata cccgcactgc tgtttgaggt ggcattcgtc aagaaaagta agccgtaaaa
    11701 gccgaccaat aatccccacc acggcatagc cggtgcgttc atttgaattg caacaaaagc
    11761 aacaactgcg gagaaagtca gggtcattgc caataacata taggtattgc gaagcacttt
    11821 gtgggtgcta attagagatt caccagttga atagctgtga gctaaacgat tttccataaa
    11881 aattccttct gtttttgtgt ttaaatcgga acatccgatg gattaatatc tatggataaa
    11941 tttccataaa tcaagaagta aggtagccgt aatttattgt aaagtcaatg cgattttgta
    12001 acagaagagt aaaaaaaaac atttagttgt gttacactta ataccactct actttatatt
    12061 ccttttcatc gatgaattcg ctatgaagct caatttccca acttatttaa ccttatttcg
    12121 ggtaggatta accccacttt ttattgtggc tttttatctg ccgacaaaat atgcgcctga
    12181 aatttcaaca cttatttttt ttattgcatc gattaccgat gcctttgacg gctatttagc
    12241 acgcaaatgg aatcaaacca cccgacttgg tgcattttta gatcctgttg cggataaagt
    12301 cttggtagct gttgcgtttg tctgcgtggc ggagtattac catacttggt ggattactat
    12361 tccaatctgc attatgattg cccgtgaaat tattatttcg gctttgcgtg aatggatggc
    12421 ggagcttggg gaaagggcca gtgttgccgt ttcgatttgg gggaaagtaa aaacgactgc
    12481 tcaaatgctc gcattaggtg gaatgttatg gagacagaac caactaatgg agattttagc
    12541 ctttattctg ctttatattg cagtgggttt aacgatctgg tctatgatgc agtatttaaa
    12601 tgcttcaaag aatagtctat tgaagtcata agcggtcata tttttattct tttttgcaat
    12661 ttgtagagtg gaaatatcgc tctacaattt tcaattttat caactctaat gtctgaattt
    12721 aacgctaaat ccttcctgtt aaatgtgcct caccaaccgg gtgtttatcg tatgtatgat
    12781 agtaaaggca cgataatcta tgtgggtaaa gccaaagatt taaaaaagcg tttagccagc
    12841 tattttcgta gccaactttc cagtaaaaaa acggaagcct tggtggcaaa tcttgctaat
    12901 attgaaacga cgatcactca ttctgaaaca gaggcgttac tccttgaaca taattatatt
    12961 aaggaaaatc agccgaaata taatgtgttg ctcagagatg acaaatctta tccttatatt
    13021 ctgttaacta aacataagca tcctcgttta acagcatttc gtggtagtaa aaagatcgcc
    13081 ggtgaatatt ttgggcctta tccgaatgtt acggcagttc gggaaacact gaatttattg
    13141 caaaaaattt ttcctattcg tcagtgtgaa gatacttatt ataaaaaccg ttcacgacct
    13201 tgcttacagt atcagatcgg acgctgttta gccccttgtg tagagggctg ttattcaccc
    13261 gttgaatacg atcagcaggt taatttggtt cgtttgtttt tacagggaaa agatcaccaa
    13321 gtagtcgagc atctggtaca gaaaatggaa gatgcggcgg aggctttgga ttttgaaggt
    13381 gcggcacgtt ttcgggatca aattcaatca gtaagagcgg tgcaagaaaa acaattcgtc
    13441 tctaatgata ggctagatga tcttgatatt atctcgatcg tttatcaaaa tgggattgcc
    13501 tgtgtgcata tcttatttgt taggcaaggt aaggtactag gcaatcgttc ttactttcca
    13561 aaagtgccaa gtaatacaga tttaaccgag ctcgcagata cctttgtcgg tcagttttat
    13621 ttacaaatga accagcaccg caccatacct aaccaaattg taattgatca tcaattaagt
    13681 gaatctaagg cactcagtga aattttaagt gagcaagcag ggcataaagt cagcatttta
    13741 gataatgcca tcagaggtga taaacagcgt tatttagctt tagcgagaac taatgcaatg
    13801 gcagcattag ttttacaact taagcaagaa tcccatattc aaagccgtta tcaggcattg
    13861 aaagaaatgt tgaaaatgtc agagattaaa agaatggagt gctttgacat cagtcataca
    13921 atgggcaacc aaacagtggc ttcttgcgtg gtgtttgatg aaaatgggcc gctaaaatcg
    13981 gattaccgcc gttttaacat tgaggggatt accggaggtg atgattatgc agcaatggag
    14041 caggctctct tgaaacgcta taatcgagaa ttaccgccag aaaagatccc tgatattatt
    14101 tttattgatg gtggcaaagg gcagttgaac cgagcgttag atgtcttcgc taaacttgat
    14161 gtggaatggg ataaatcgaa accgctttta attggtgtgg caaaaggcgt agaacgtaaa
    14221 gctgggctgg aaactttaat tatcagcaaa tgggataaag aaattcactt accgccagat
    14281 agtcttgcct tgcatttaat tcagcatatt cgagatgaat cccaccagca tgccattagt
    14341 gggcatcgta aaaaacgcca aaaggccttt accgaaagtg gattagaatc tattgccggt
    14401 gtcggtgcaa aacgccgaca agcgttgcta aaatatttag gcggaatgca gggggtgaag
    14461 tctgcaacct tggaagagat tcaatcagtt ccgggaattt ctaaagcgtt ggcagaggtt
    14521 atttttgata cattaaaaaa tagttaaatt atcaatagcg atatcgcttc cagccgtaat
    14581 ggtagcccac tcctatgctt gggtaaatta tagggtgaat tggcagattt atgctttctg
    14641 agcgtttatc gctttggttt aatggctcat catgttgctt tgtattttta tcaacagttt
    14701 gcttattgat tactcgttga tggtaatcct gaactgcttg catatccatt ggtactgtgc
    14761 tttgttgttg gggtgtcgaa caggcggtta gtgcaacgct caatccgagt aaaattaatt
    14821 ttttcatatc atttccttat attgcaaaat attttgcttt tttgaccgct tattttgcta
    14881 ttaattctta aaaagattat tttatccata aaaaaggacg cactaggcgt cctcatttct
    14941 attctttaaa attaaagagc agattttgct ttttcaacta aagctgcgaa agcaactttg
    15001 tcaaatactg cgatgtcagc taagatctta cggtcgattt caacagatgc ttttttcaag
    15061 ccgttgatga atttgctgta agataaaccg ttttgacgag ctgctgcgtt aatacgagca
    15121 atccataatt gacggaattg acgtttacgt tgacggcggt cacggtaagc atattgagca
    15181 gctttaacta ctgcttggaa agcaacgcga tacacgcgtg aacgtgcacc ataataacct
    15241 ttagcagcct taagaacttt cttatggcgt gctcttgcaa taacaccacg ttttacacga
    15301 gccattattt aatctcctaa ttgtctattt tgattggtgg tcgtcatcac gacctttact
    15361 aagaattcta tccgtacgtc cgtactttac tacactacgc taacctaaaa ggcttatgcg
    15421 tatggtaagc acgctactac taaaacttgg tcagctttcg ctaccattga tttgtgacgt
    15481 aattgacgtt tacgtttagt ggttttctta gtcaaaatgt gacgtaagtg agattgttta
    15541 cgtttaaaac cgccggaagc tgtttttttg aaacgcttag cagcaccacg tactgtttta
    15601 attttaggca ttgttaaaaa actccgcatt gtttgttaga tattatgaaa ataaggcgaa
    15661 ttttgtgaac aaaattactt ggaagcactt attctccgct atacgatttt catcgttaaa
    15721 aagtcaagca ggctgctgag tttgcctgtt gagcattttc tctttcttgg ctaggcaaaa
    15781 aagcttgcga attttagcga aaatataacc gctatgcaag ccaatttaag ctttatcaag
    15841 aaaaaagcac attaaaacaa tgtgcttatc gaaattattt cttcttaggt gcgatcacca
    15901 tcacaacttg gcgaccttcc aatttgcctg gggctgactc taccattgca atttctgcag
    15961 tatctgcttt gacacgttct aaaacgtcca taccgatttc ttggtgagcc atttcacgac
    16021 cacggaaacg cactgtgatt tttactttat ccccatcttc taagaaacgg ataatgctac
    16081 gaagtttaac ttgataatcg ccatcatcag ttcccggacg gaatttgatc tctttaactt
    16141 gaacaacttt ttgttttttc ttttgttctt ttgctgcttt ctctttttcg tagatgaact
    16201 taccatagtt cataatacga caaactggtg gttcagcatt tgggctgatt tccactaaat
    16261 ccaattcagc ttcttctgct ctatttagag catcttgaag tgatacaata ccaacttgtt
    16321 cgccgttttc atcggttaag cgaacttctt ttacgccacg aatttcgtca ttaagacgat
    16381 gtgcacgatt tgattgaaca cgttttacag gtttaatagc cttattcctt ctagtaaatt
    16441 tttgcttaaa ttttaccgct tgagagcgta taaatatttt agattgcttg ttttaagtga
    16501 ttaaaaaaca gctaaacgtg cggattctat aagattttca gtgcttatgc aataataaag
    16561 cccttattta actgcctatt ttcggttatt ttgatattct ttgattaaac tcataaatgc
    16621 ttgcccaaat cgttcaaatt ttcgttcacc aacaccatta atttctaaca tttccacttt
    16681 tgaagtcggg agaaattctg ccatttcttg caaggtggca tcgttaaaga cgatataagg
    16741 tggaatattt tcgaggtcag ctatctgctt gcgtaaaaaa cgtaagcgtg cgaataggtc
    16801 tttgtcatag cgatgacttg cttgtttttg cacaaatgca gtcgcagata tacctaaacg
    16861 tggcaatgca agctgaagtt gcttttctcc acgcaaaaat ggtctggcac tttcggttaa
    16921 ttgcagagcg gaatggttag caatattttg aataatcaac cctaaatgaa tcagttgccg
    16981 caccacattt tgccaataat gttggctttg ttcttttccg ataccataaa cggaaagttg
    17041 atcgtgttga tgttcacgaa ttttgagatt gttcattcct cgcaataccg caataatatg
    17101 gtgctgaccg aaaatttgac cggtgcggta aatcaccgac atcacttttt gggcatcaag
    17161 taagccatcg tattgttgcg gtggatctaa acaaatatcg cagtttttgc attgctcttg
    17221 gcgatgttcc ccaaagtagt tgagtaagac taaacggcgg caagtctgtg attcggcgaa
    17281 atcgccaatt gcctgtaatt tatgctgttt aatatttctc tgttcgcttt ccggctcttc
    17341 caataatatt ttttgtaacc aagcataatc gctaggatca taaaaaagca cggcttcaga
    17401 agggagatca tctcgcccgg ctcgtccggt ttcttgatag taagattcga tagagcgagg
    17461 taaatcgaaa tgtaccacaa aacgtacgtt ggatttgttg atccccatac caaacgcaat
    17521 agtggcaacc acaatttgaa tatcatcacg ctgaaaggca ttctgcactc gttctctctg
    17581 ttcaaaactc ataccggcgt gatagcccat cacgctgatt ttacgggctg agagtttctc
    17641 ggtgatttct tccacttttt tgcgactgtt gcagtaaaca ataccgcttt tgccttgctg
    17701 tttgctgatg aatttaatga gctgttcaag cggtttaaat ttttcctgca cggtgtagcg
    17761 aatattgggg cgatcaaagc tgcctaaata gatatgtggg ctatttaagc gtaaatgatg
    17821 cagaatatcg gagcgggttg ttagatctgc cgtggcggtg agtgccatca acggcacatt
    17881 cgggaaagtg tttcgtaagc cacctagcag ggtatattca gggcggaaat cgtgccccca
    17941 ttgggaaaca caatgggctt catcaaccgc aataaagctg attttacata gcgaaataaa
    18001 gtggaaaaag ccttgagtca ttaccttttc aggggaaaga tagagtaatt ttaattgacc
    18061 ggataaggct ttttgttcca caagttgctg ctcttcgagc gtttgagtgg agttaagata
    18121 agctgcttca atgccgttgg tgataagttg atccacctgg tctttcatca acgaaataag
    18181 cggcgaaatc accaaagtga tgccgtctag gcacaaagcc ggcacttggt agcaaaggga
    18241 tttgccaccg cctgttgtca taatcacgag gcaatcacga ttttccaaca cggcattgat
    18301 aacttcttct tgcccgtctc ggaatttttg atagccaaaa atattattta aaacagaaat
    18361 tgcggtggtg ttcattggta gttggttgct acttatgata gtactcgtct tctgagagta
    18421 tcttttaaaa tgaaaataaa gcactcatca aagacgagtg ctgtcttagt taacaattag
    18481 gcattagaaa gaaacgcttt ctccgtgtcg agcaaaagct tcttctaaat gttgccagaa
    18541 cgagcgacct tcatctgtta cccattcgcc ttctacattt ttaaagtgga aaccacccaa
    18601 tttgctggca agccataatt ccagcattgc ctcttgttta ttaatgataa cttgcgattc
    18661 atcatcaaag gttaaggtgc agaccgctcc ttggatttca gtatcgactg ataatccttc
    18721 gtcataaatt ttttcttcaa tttgttgcca tacttgctct acggcttggt ggaattgtgc
    18781 tgtgttcatt ggtgtatcct tatttgaatg ttatataagt agtaaatact ttttgtttta
    18841 gctgtttaaa tggtctgtaa agttcttttt gtatttttgt taagaaataa ttaggctttt
    18901 taagtttagc ttgatttttt att
//
LOCUS       DANLYI010000028        18141 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.028, whole genome shotgun sequence.
ACCESSION   DANLYI010000028 DANLYI010000000
VERSION     DANLYI010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 18141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18141
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.028"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            complement(81..4067)
                     /gene="hrpA"
                     /locus_tag="PXD81_000733"
     CDS             complement(81..4067)
                     /gene="hrpA"
                     /locus_tag="PXD81_000733"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HDL5536786.1"
                     /translation="METPLQKNHKKPPLVKLENITTADFRRLLGRLKGAENIKNIDHK
                     RSVLAEISQDLTACQARFFARTQHAQNLKIDYPDLPVSARREEILKLISENQVVVIAG
                     ETGSGKTTQLPKMCLELGRGVKGLIGHTQPRRIAARSVATRIAEELKSELGETVGYKV
                     RFNDQVSENSLIKLMTDGILLAEIQHDSYLNQYDTLIIDEAHERSLNNDFILGYLKQI
                     LHKRPDLKVIITSATIDVERFSKHFNNAPIIEVSGRTFPVEVRYRPIVEDQDQLQGIL
                     NAVDELQAEGRGDILIFMNGEREIRDTAEALQKQELRHSEILPLYARLSAAEQQRIFQ
                     PSGGLNRIILATNVAETSLTIPNIKYVIDTGTARISRYSYRTKVQRLPIEPISQASAN
                     QRKGRCGRTSEGICIRLYSEEDFNSRPQFTDPEILRTNLAAVILQMTSLGLSDIANFP
                     FVDAPDSRQIQDGIRLLEELQAIIPNEKIPSTACGGGLGRGEIRSNDGTPPLPTSPRK
                     RGDGFRLTPIGRQLAQLPVDPRLGRMVIEAAKNGSLHEVMMIVSALSIQDPRERPQEK
                     QQSADDKHRRFADKESDFLAFVNLWHFIQAQQKELSKNQFRNLCRKDYLNYNRVREWQ
                     DIYHQLRLAVREMGLKINSEPASYQPIHTALLAGLLSHIGLKDNEKMHYLGARNAHFF
                     IFPNSALFKKQPKWVMAAELVETTKLWGRMVARIEPEWVEPLAVHLTKSSYSEPHWAK
                     SKGAVMASEKVSLYGIPIVAQRSVNYGSIDPTVSREIFIRSALVEGECHNNYKFFKEN
                     HRLIREVEALEHKSRRRDILVDEQVLFEFYDQRIGTEVVSSKHFDTWWKQASKKDPEL
                     LNFEKSFLINDNANLASELDFPNFWHQGDLKLKLSYQFEVGKDHDGVTVHIPLPLLNQ
                     VEPEGFDWQIPGLRHELIVSLIKSLPKSLRRNFVPAPNYADAFLGRAEPFKKPLLDNL
                     SHELRRMTGVLVEPEQWDLSQLPPHLRMTFRVIDDKGKKLQESENLDELKFALKDEVQ
                     NTLSNIADDGIEQSGVHIWNFAELPQFYEQKKAHFSVKAYPAIVDEQTAVGVKLFETE
                     FEQARAMQAGLRRLLLLNVPSPIKYLHEKLPNKAKLGLYFAPFGKVLELIDDCIACAV
                     DQLVEEFGGFVWTEEKFTQLHEFVRANLNDKTVEIAKQVEKCLTLAYELNKRLKGKLD
                     FTMAFALSDIKTQIEGLIYPDFVTKTGYARLADLYRYLTAIERRLDKLLVDSNTDRAK
                     MLRVEAVQEAYKQLLAKLPKSKAVPDEVLEIRYMIEELRVSLFAQQLGTKYPISDKRI
                     LNVINEIK"
     gene            complement(4171..4623)
                     /locus_tag="PXD81_000734"
     CDS             complement(4171..4623)
                     /locus_tag="PXD81_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HDL5536787.1"
                     /translation="MTLQFNAVALLLVVLIILGLISQNSAVTISAAVLLIMQQTLLSK
                     FIPFVDQYGLKIGIIILTIGVLSPLVSGRITLPELAQLLNWKMALSIIAGVLVAWLGG
                     RGVNLMGSQPVLVTGLLIGTVIGVAFLKGVPVGPLIAAGILSLIVGKT"
     gene            complement(4686..4955)
                     /locus_tag="PXD81_000735"
     CDS             complement(4686..4955)
                     /locus_tag="PXD81_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5536788.1"
                     /translation="MDAAMSLRFDKLRFVKRLQEANQTPEMAEAFANALDEALEQSQS
                     PLATKADLQLELEKLKNEINTTIFKAITLNITILGFLMAIMKFIN"
     gene            complement(5053..5649)
                     /gene="grpE"
                     /locus_tag="PXD81_000736"
     CDS             complement(5053..5649)
                     /gene="grpE"
                     /locus_tag="PXD81_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005712850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HDL5536789.1"
                     /translation="MTTQNEHLQEEVKQEEIQPEVVTEETQAEEQNTPQEEPLALAQA
                     RIAELEAYIAEADKREQDIQLRAQAEIQNIRRRAEQDVEKAHKFALEKFSKELLTVVD
                     NLERGLDALDKAVTDETTQALVDGVEMTHKEFINTLAKFGVVAIGEVGEAFNPELHEA
                     ISMQPAEGIEANHVSTVLQKGYTLHGRVIRPAMVMVAN"
     gene            5871..6965
                     /locus_tag="PXD81_000737"
     CDS             5871..6965
                     /locus_tag="PXD81_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HDL5536790.1"
                     /translation="MKKLAGLFAAGLATVALTACNEEKAAEPKATAAEAKPQATNQTV
                     HLYTWTEYVPEGLLDEFTKETGIKVEVSSLESNETMYAKLKLQGKDGGYDVIAPSNYF
                     VSKMAKEGMLAELDHAQLPVIKELNQDWLNKPYDQGNKYSLPQLLGAPGIAFNTADYK
                     GETFTSWGDLWKPEFAGKVQLLDDAREVFNIALLKLGKNPNTTNPDEIKEAFEELKKL
                     RPNVLSFTSDNPANSFIAGEVSVGQLWNGSVRIAKKENAPVDMVFPKEGPVLWVDTLA
                     IPANAKNKENAHKLINYLLSAKVAEKLTLAIGYPTSNVEALKVLPKEITEDTAIYPSA
                     EVLQKSQWQDDVGDAIELYEKYYQELKAAK"
     gene            7101..8492
                     /locus_tag="PXD81_000738"
     CDS             7101..8492
                     /locus_tag="PXD81_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HDL5536791.1"
                     /translation="MNLRWQEYPENARKLFKLSLPIFISQLSASGMGLADIVMAGLVS
                     DDDVSAIAVSNSIYFPLFLFVSGLLNAITPTVSYLNGSSNRHLIANQVRQGYWIVFAM
                     SIPLMAIFLNSHLILDLMQTPAEFSIKSQQYLAVMAVGLIPALLAVSLRCMNDGLSNP
                     KPAMRITFFGLLLNIPLNYIFIFGKFGLPEMGAVGCGVATAIVNLIMFFMLLHYCYTN
                     SAQKDIRLFEKWIDAPSGQILLKLCKLGLPIAFAIFTEVMLFSTSSLFLSPLGSQVVA
                     SHQAALQTSSLFFMIPMSFGIATTIMIGQTLGQKNVEGAKILSYHALITATALAIIAA
                     IIIILLKNFIPFAFTNNPISIAITAHLLIFAAVYQIPDAIQAVANGILRGYKHTQPIL
                     YVTLFCYWGVSMPLGYILARTDWIVQPMAAQGFWLVFCIGLSLAGGLLLWQMRKIQAV
                     PKAQLIAKLERIK"
     gene            8551..12075
                     /gene="recB"
                     /locus_tag="PXD81_000739"
     CDS             8551..12075
                     /gene="recB"
                     /locus_tag="PXD81_000739"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HDL5536792.1"
                     /translation="MKNLNPIQLPLNKSVLIEASAGTGKTFTIANLYLRLLLGIGCNP
                     LTVEQILVVTFTKAATEELKNRIRKNIQQCADFLKDQADGLEVESTKSYRNNLDFLAQ
                     IYPLIPNIHEALLRLSIAEREIDTASVFTIHGFCQKMLVQFAFESGVRFDLDLQPNQS
                     DLLKKLSEEVWREQFYPQDLAITYAVAEQLGTPEYALNAVRRYLSTELPEPNASLNQD
                     IAEHLAQYQQFINEVKSYWKTHSDELVETIRHDLKHKITLHGGWYRATTFEAGVEVMN
                     QWANSQYNHFPAGLYLFTTEKIAAGTKKSCEPIDSPHLAKNQAFLTAYKERFENSLSA
                     MLQYQFLIALKAKLAEYKQSHPQRSFDDLLTMLNQALKNLHTGEALSQKIRAMYPFAM
                     IDEFQDTDLTQYEIFSQIFMAENAENGFIMIGDPKQSIYKFRGADIFTYLRAAKQADE
                     QFTLGKNWRSLPEVVQAVNNLFSFANDNSPFLYEDIGFHPVEADSQKGQLVSEQGHFV
                     GYITENSKLDDLAEMCAYQIHQQLEAMAQGKFGVEKNGEFQSFAAKDIAILVRKGSQA
                     KRIKKALAKRNIKSVYLAESASVFGSEIATELCWLLQAALNPYHYKALLSALGSTLWG
                     LTAAEIHRYKENEQLWDLKVNQFVEYSNIWKEQGVLPMLHKIYLEQGIIERLKGLENA
                     DRLITDLLHLTEILQETSQALESEAELLRWYEKQVNSPDFNDEQQLRLESEEELIKIV
                     TIHGSKGLQYPIVWLPFVSQKSEGDTQTALSLYRNSQGALDWCLGSEEASFSLQYEAY
                     AEDLRLLYVALTRAESQLNLVLPKEISGWSPLHYLLADGEIPTGKVPVSDYFSQKRIA
                     ANLVEVAGELPFSLWEGEQSSDDYQAKTFSGNIRVVGQITSFTSLYAQHERFSEGKSE
                     RVMDLAQDRDVHIETIEAGYSSETAAFSPFQFPHGIKVGTVLHHFFEHCQFNEQIDRE
                     AVAKVCEQLGLSEEWIEPTALWFERILTTPLAEANFCLKAIDETKRLNEWQFYLRLKN
                     DKALHQLNALLKQHSPLAKTLPELQLPQLEGFVRGFVDCIVQVEEKFYLIDYKSNFLG
                     YLPQNYAKEHIQREMGRQRYDLQYLLYTLALHRYLTARLGEKYDYERDFGGVAYLFLR
                     AMNGENDNGVYFDKPTKQLIEEMDRLFG"
     gene            complement(12117..12599)
                     /gene="greB"
                     /locus_tag="PXD81_000740"
     CDS             complement(12117..12599)
                     /gene="greB"
                     /locus_tag="PXD81_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HDL5536793.1"
                     /translation="MAKSNYITRSGWQALDQELKFLWREERPKVTQAVSDAAALGDRS
                     ENAEYIYGKRRLREIDRRVRFLSKRLEVLQIVDYHPKQEGKVFFGAWVELENEEGEAK
                     QYRIVGCDEFDPAKNWISIDSPVARALIGKQVDDEIRVETPLGKVLLYINKIWYEQAS
                     "
     gene            complement(12650..13930)
                     /gene="pepB"
                     /locus_tag="PXD81_000741"
     CDS             complement(12650..13930)
                     /gene="pepB"
                     /locus_tag="PXD81_000741"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HDL5536794.1"
                     /translation="MTAMKISLSTQNAPEIWGKNAILSANDAGFTVHNPNNDLATIQK
                     AARKIKNQGISTVELTGDSWDLESCWAFHQGFHSVKNQGSVQYPDLDEQQAEFDNRVK
                     CATFVRELINKPSDKLTPEKLAEQAVEFIQKMAKPNTVSYQIIRGEALQEQGYHGIWT
                     VGKGSANPPAFLVLDFNPTNDENAQVLACLVGKGITFDTGGYSLKPSDSMSTMRTDMG
                     GAALLTGSLAFAIARGLNKRVKLYLCCAENMVSHNAFKLGDIITYRNGVTVEVLNTDA
                     EGRLVLADGLIDACKQNPQFIIDAATLTGAAKVAVGNDYHSVLSMDDNLTEALFKSAK
                     TENEPFWRLPFEEFHRGQIASSFADIANIGSVPVGAGASTATAFLSYFVENYQQNWLH
                     IDASATFRKTASDLWAAGATGLGIRTVANLLLSK"
     gene            complement(14069..14890)
                     /locus_tag="PXD81_000742"
     CDS             complement(14069..14890)
                     /locus_tag="PXD81_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010869033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase, lipoprotein e(P4) family"
                     /protein_id="HDL5536795.1"
                     /translation="MKNIKLSAMSLTALFVLTACSHTHKNSDEHHQMVLQEQATLGIN
                     WMQQSGEYQALAHQAFNAAKVAFDQAKVKKGKKKAVVVDLDETMVDNSLYAGWQVKNH
                     KPFDGESWTRWVNARQTGAIAGAVEFNNYVNSHKGTMFYVSNRKDSSEKAGTIDDMNK
                     LGFSGVSEQTLLLKKDKSNKTPRFEEIEKQGYEIVLYIGDNLNDFGDATYRKSNAERR
                     AFVAENSQQFGKKFIMLPNPNYGDWEGGLDKDYYKGNAESRVKIRHNAIKAWDGK"
     gene            15038..15981
                     /locus_tag="PXD81_000743"
                     /pseudo
     CDS             15038..15981
                     /locus_tag="PXD81_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251085.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
     gene            complement(16094..17257)
                     /locus_tag="PXD81_000744"
     CDS             complement(16094..17257)
                     /locus_tag="PXD81_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HDL5536796.1"
                     /translation="MKRKTLATLVLIAALGIGGYFYYQQQTNKPQIHYITQAVTRAEI
                     NKNVLATGSVRANKRTEVGAQVSGKIQKLYVELGQSVKQGDLIADIDSSNQSNTLSTA
                     QARLSSYKAQLTSAQVALEVAQSNYNRLNKLYRANSASLNDLETAKNTLASAKATVDN
                     IKAQLKSAEISVNDAKTNLTYTQIISPMDGVIVSVPVSVGQTVNSNQTSPTIVQVADL
                     SKMLIKLEISEGDIAQVKVGQKVKFSTLAEPNREYSATIDSVDPALTTLTDNNYTEQS
                     GNTEAVYYYANVSVDNADNSLRIGMTTQAQITIDKREQVLVVPTSVIKKRGKENYVLV
                     LENEQSVERKIQIGISDTQNTEVLAGLNEGDQVIVTQRADDEKISTARGPRMF"
     gene            complement(17323..18138)
                     /locus_tag="PXD81_000745"
     CDS             complement(17323..18138)
                     /locus_tag="PXD81_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005600497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDL5536797.1"
                     /translation="MTLPFRAIISDLDGTLLNANHKIGDFTIDTLEQLSKKGVDIFFA
                     TGRNHPDVSQIIGKVNVKNAMLVTSNGARANNLEGKKILNHYLPEDLAFELMNIEFDD
                     QNVCLNSYQGDEWFINKEIAELKKFHQDSGFSYQVVNFAHHHGRNTEKVFFIGKTAEA
                     LVPIEHYIRDTYGDRVQMTYSALLCLEVMAKGVCKANALAELVKLRGYTLADCIAFGD
                     GMNDVEMLSLAGKGCLMGNADPRLKAVLPNNEVIGFNKDEAVASYLRATFGIV"
BASE COUNT         4905 a         3796 c         3945 g         5495 t
ORIGIN      
        1 gaggaatagg tattcgagaa aacaagcggt cagagttgca aaatttttta cttatctgac
       61 cgcttgttgt atagggaata ttactttatt tcattgatca cattcaaaat ccgcttatcc
      121 gaaatcgggt attttgtgcc gagctgttga gcgaacagac tgacccgcag ttcttcgatc
      181 atatatcgaa tttccagtac ctcgtccggc accgctttgg attttggcag tttggcaagg
      241 agctgtttat atgcctcttg cactgcttcc acccgtagca ttttggctcg gtcggtgttg
      301 ctgtccacca acagtttatc caaacggcgt tcgatagcgg tcaaataacg gtataaatct
      361 gcaaggcggg cgtagccggt tttggtgaca aagtcggggt aaatcagccc ttcgatttgg
      421 gttttgatgt cgcttaacgc aaacgccatc gtaaaatcga gcttgccttt taaccgcttg
      481 tttagctcgt aggccagtgt gaggcacttt tcaacctgtt tggcaatttc tacggtttta
      541 tcgtttaaat ttgcccgcac aaattcgtgc agttgggtga atttttcctc cgtccagaca
      601 aacccgccga actcttctac cagctgatct accgcacagg caatgcagtc gtcaatcagc
      661 tccaatactt tgccgaacgg ggcaaaatat aagcccagct tggctttgtt cggcagtttt
      721 tcgtgtagat atttaatcgg cgatggcaca tttagcaaca gcagacggcg aagtcctgcc
      781 tgcattgcac gagcctgctc aaattccgtt tcaaacagtt tcacccccac agccgtttgt
      841 tcatcgacaa tcgcagggta ggctttcacg ctgaaatggg cttttttctg ctcatagaac
      901 tgtggcaatt cggcaaaatt ccaaatatgc acaccacttt gctcaatgcc gtcatcggca
      961 atgttggaaa gggtgttctg cacctcatct ttcaaggcaa atttcagctc atcaaggttt
     1021 tcgctctctt gcagtttctt gcctttatca tcaatcacac ggaaagtcat ccgcaaatgt
     1081 ggcggcagtt ggcttaaatc ccactgttca ggctcgacca atacacccgt cattcggcgt
     1141 agttcgtggc ttagattgtc cagcaacggt tttttaaacg gctcggctcg ccctaaaaag
     1201 gcgtccgcat aattcggcgc cggcacaaaa tttcgccgta gcgatttcgg taaagattta
     1261 atcagcgaga cgatcagctc gtggcgtagc cccggaattt gccaatcgaa accttcgggt
     1321 tccacttggt taagcagcgg tagcggaata tgcaccgtca cgccatcgtg atcttttccg
     1381 acctcaaact gatagctcag tttgagtttt aaatcgcctt gatgccagaa attcgggaaa
     1441 tccagctcgc tggcaaggtt ggcgttgtcg ttgatcagga acgatttttc aaagttcagc
     1501 agttcaggat cttttttgct tgcctgtttc caccaagtat caaaatgctt gctcgacaca
     1561 acttcggtgc caatccgctg atcgtaaaac tcaaacagca cctgctcgtc caccaaaata
     1621 tcacggcggc gggatttgtg ttccaatgcc tcaacttcac ggatcaagcg gtgattttct
     1681 ttgaaaaatt tgtaattgtt gtgacattcg ccttccacca acgccgagcg gataaaaatc
     1741 tcacggctga cggttgggtc tatcgagcca taattcaccg agcgttgtgc cacaatcgga
     1801 atgccgtaga gcgacacttt ttccgatgcc attaccgctc cttttgactt cgcccagtgt
     1861 ggttcgctgt aactggattt agtgaggtgt actgccaacg gttccaccca ttccggctca
     1921 atgcgagcga ccattcgtcc ccaaagtttg gtagtttcca ccagctctgc ggccattacc
     1981 catttcggct gttttttgaa aagtgccgag ttcgggaaaa tgaaaaagtg ggcatttctt
     2041 gctcctaaat agtgcatttt ttcattatct ttcaagccga tatgcgacaa caagccggcg
     2101 agcagggcgg tgtgaatcgg ctgataactt gccggctcac tgttaatttt taagcccatt
     2161 tctctaactg caaggcggag ttggtggtag atatcctgcc actcccgcac acggttgtag
     2221 ttcaaataat ccttacggca gagattacgg aactggtttt tcgacagttc tttttgttgg
     2281 gcttggataa agtgccacaa attcacaaag gctaagaaat cgctctcttt atcggcaaaa
     2341 cggcggtgtt tatcgtctgc cgactgctgt ttttcctgcg gacgctcacg agggtcttgg
     2401 attgagagag ctgacacaat catcatcact tcgtgcaacg aaccgttttt cgccgcctcg
     2461 atcaccattc tgcctaagcg aggatcgact ggcagttggg cgagttggcg accgattggg
     2521 gttaatctaa acccatcccc ccgtttacgg ggggaggttg ggaggggggg agtgccatca
     2581 ttagaacgga tttcccccct ccctagccct cccccgcaag cggtggaggg gattttttca
     2641 tttggaataa tcgcctgtaa ttcttccaac aaccgaatcc cgtcctgaat ttgtcggctg
     2701 tcaggggcat caacaaacgg gaaattggca atatcactca atccaagcga agtcatttgt
     2761 aaaataaccg ctgcaaggtt agttcgtaga atttcaggat cggtaaattg tggacggctg
     2821 ttgaaatcct cttctgaata gaggcggata cagatccctt ccgaggttcg cccacaacgc
     2881 cctttccgct ggttggcgga ggcttgcgaa atcggctcaa tcggcaagcg ttgcactttg
     2941 gtgcggtagc tataacgaga aatacgagcc gtgccggtgt cgatcacata tttaatattc
     3001 ggaatagtga gggaggtttc tgccacgttg gtcgccagaa taattcggtt tagcccaccg
     3061 cttggctgga aaatccgttg ttgttcggca gccgataagc gggcgtaaag gggtaaaatt
     3121 tcgctatgtc gcagttcctg cttttgcaag gcttcggcag tgtcacgaat ttcccgttca
     3181 ccgttcataa aaatcagaat atcgccacgc ccttcggctt gcagttcgtc caccgcattt
     3241 agaatacctt gcagttgatc ttgatcttcc actatcgggc gataacgcac ttccaccggg
     3301 aatgtccgac cagagacttc aataatcggg gcgttgttga aatgtttgga aaaacgttct
     3361 acatcaatag ttgccgaggt gatgatcact ttcagatcgg ggcgtttgtg cagaatttgt
     3421 ttcagataac ccaaaataaa atcgttgttc agcgaacgct cgtgagcttc gtcaataatc
     3481 agcgtatcgt actgatttaa atagctgtcg tgctggattt ccgccagcag aataccgtcc
     3541 gtcattagct tgattaagct gttttcactg acttgatcgt taaagcggac tttgtaaccg
     3601 accgtctcgc ccagttccga tttcagctct tcggcaatgc gagtcgccac cgaacgtgcg
     3661 gcaattcggc ggggctgggt atgcccaatc aagcccttca ccccacgccc gagttctaag
     3721 cacattttcg gtaactgggt ggttttgccc gaaccggttt cccctgcgat caccaccacc
     3781 tgattttcgc taatcagttt taaaatttcc tcacggcgag cggaaacagg cagatcaggg
     3841 tagtcaattt tcaggttttg ggcgtgctga gttcgtgcga aaaaacgtgc ctgacaagcg
     3901 gtgagatctt gcgaaatttc tgccaaaact gaccgcttgt gatcgatgtt tttaatattt
     3961 tccgcccctt ttaagcgacc gagcaggcgg cggaaatcgg cggtggtgat attttccagc
     4021 ttcacaagcg gtggtttctt atgatttttt tgcaaaggtg tttccattta atttctcttt
     4081 tgcattaaga ccttgtgatt tattgtaggg tgggcgtaag cccacgtaac agaataggga
     4141 ttttatgcgt ggacttacgt ccaccctaca ttaggtttta ccaacaatca gtgacaaaat
     4201 ccctgccgca atcaacggcc cgaccggtac gccttttaag aacgccacgc cgatcaccgt
     4261 gccgattaaa agcccagtta ccaacaccgg ctggctgccc attaaattca cgcccctgcc
     4321 acctagccac gctaccagca cgccagcaat tattgagagt gccattttcc agttcaatag
     4381 ttgggcaagt tcgggaagcg taattcgccc tgaaaccaag gggctaagta cgccgatggt
     4441 taaaatgata atcccgattt ttaagccgta ttgatcgaca aaggggataa atttagaaag
     4501 taaggtttgt tgcataatca gcaatacggc ggctgaaata gtaaccgcac tgttttggct
     4561 gatcagccct aaaataatca gcaccacaag tagaagtgcc actgcgttaa attgtaaagt
     4621 cataggatta aaacgtaaaa acccttactt catagaagta agggagtggt taattaaacg
     4681 ttgagctagt taataaattt cattattgcc attaaaaagc caagaattgt gatatttaag
     4741 gtaattgctt taaatatcgt ggtattgatt tcatttttta gcttttcaag ttcaagttgc
     4801 aaatcagctt ttgttgctag tgggctttga ctttgctcta aggcttcatc gagagcatta
     4861 gcaaatgcct cagccatttc aggtgtttga ttggcttctt gcaagcgttt tacaaaacgt
     4921 aatttgtcaa atcgtaggct catagctgca tccatttcaa ttatcctctt gtggttaagt
     4981 tataaactaa tttagcataa tcacaagcag gaattttagg agattttgtg atcttcgtcg
     5041 aaaatcgaca atttagtttg ccaccatcac cattgccggg cgaatcactc gtccgtgaag
     5101 cgtgtagcct ttttgtagca ctgtgctgac atggttggct tcaattcctt cggcaggttg
     5161 cattgaaata gcttcgtgca attcagggtt aaatgcctcg cctacttcgc caattgccac
     5221 cacaccaaat ttagccagtg tattgatgaa ttctttatga gtcatttcaa cgccgtcaac
     5281 taacgcttga gtggtttcat cggtcacggc tttatcaagg gcatctaatc cacgttcaag
     5341 gttatcaacc actgttaaca attctttgga gaatttttct aaagcgaatt tgtgagcttt
     5401 ttcaacatct tgctctgcac ggcggcggat attttgaatt tctgcttgag ccctaagctg
     5461 aatatcttgc tcacgtttgt cggcttctgc aatataggct tctaactcgg caattcgagc
     5521 ttgagcgaga gctaacggtt cttcttgagg tgtattttgt tcttccgctt gagtttcttc
     5581 agttacaact tcaggttgaa tttcttcttg ttttacttct tcttgtaagt gttcattttg
     5641 agtggtcatt ttttatcctt taaaagacta atctggaaat gtaaatcttc cttaatatag
     5701 cgataaatta agggatttca agcatatctc tttctttttg cgaaaataat tcacttttta
     5761 atcagaaaag gcttgcaaaa tacggggggg ggcttaaaat gccctagtct tttccgccct
     5821 taggcaaggc tatatttttt aatcactttt ctttaacgga gaaaacttaa atgaaaaaat
     5881 tagcgggttt atttgcagca ggtttagcaa cagttgcatt aacagcgtgt aatgaagaaa
     5941 aagcggcaga gccaaaagca actgcggctg aagcaaaacc tcaagcaaca aatcaaacag
     6001 ttcaccttta tacttggact gaatatgtgc ctgaaggttt gttagatgaa tttacgaaag
     6061 aaaccggcat taaagttgaa gtttcaagtc ttgaatctaa cgaaacaatg tacgctaaat
     6121 taaaattaca aggcaaagat ggcggttatg atgtaattgc tccatcaaac tactttgtat
     6181 ctaaaatggc gaaagaagga atgttagcgg aattagatca cgcacaactt ccggttatta
     6241 aagaattaaa ccaagattgg ttaaataaac cttacgatca aggaaacaaa tattcattac
     6301 ctcaattatt aggtgcgccg ggtattgcat ttaatacggc agattacaaa ggtgaaacat
     6361 tcacttcttg gggtgatttg tggaaacctg aatttgcagg taaagtacaa ttattagatg
     6421 atgctcgtga agtattcaac attgcgttat taaaattagg taaaaaccca aatacaacta
     6481 atccggatga aatcaaagaa gcctttgaag agttgaaaaa attacgtcca aatgtgcttt
     6541 catttacctc tgataacccg gcaaactcat ttattgctgg tgaggtttct gtaggtcagt
     6601 tatggaacgg ttctgtacgt attgcgaaga aagaaaacgc accggttgat atggtattcc
     6661 cgaaagaagg tcctgtgctt tgggtcgata ctttagccat tccggcaaat gcgaaaaaca
     6721 aagaaaatgc acataaatta atcaactact tattaagtgc aaaagtagct gaaaaattaa
     6781 cgttagcgat tggttaccca acttcaaacg tagaagcatt aaaagtatta ccgaaagaaa
     6841 ttactgaaga tacagcaatt tacccatctg cagaggtttt acaaaaatct caatggcaag
     6901 atgatgttgg tgatgcaatt gagctttacg aaaaatacta ccaagagtta aaagcagcaa
     6961 aataatagct gattaattct gaagcctcta gggagcaatc tttagaggct ttctcttttg
     7021 agttgcaaaa aatttggcaa aaacgaccgc tttctaaggt acaatcataa aaacatactt
     7081 agattttgag agattaaacc atgaacttaa gatggcaaga atatcctgaa aatgcccgca
     7141 aattatttaa actatcccta cccattttta tttctcagct ttcagcttcc ggaatggggc
     7201 tggcggatat tgtaatggct ggcttggtga gtgatgatga tgtctcggcg attgcggtaa
     7261 gtaattcgat ttactttccg ctctttctgt ttgtgtcagg gttgctgaat gcaattaccc
     7321 caaccgtttc ttacttaaac ggctcgagca atcgccattt aattgctaac caagtaaggc
     7381 agggctattg gattgtgttt gcaatgtcta ttccattaat ggcaattttc ctaaacagcc
     7441 atctgatttt agacttaatg caaacaccgg ctgagttttc aatcaaatcg caacaatatt
     7501 tagcagtaat ggcagttggg cttattcctg ctttgctggc ggtgagttta cgttgtatga
     7561 acgatggatt atctaaccct aaacctgcga tgcggattac ctttttcggt ttattgctga
     7621 acattccgct aaactatatt ttcattttcg gcaaatttgg cttgcctgaa atgggagcgg
     7681 taggctgtgg tgtagcaacc gcaattgtga acttgattat gttttttatg ttgttgcatt
     7741 attgctacac caattctgcc caaaaagata ttcgtttgtt tgagaaatgg attgatgctc
     7801 caagcggtca aattttgcta aaactttgca aattagggct gccgattgcc tttgcgattt
     7861 ttaccgaagt gatgctgttt tcgacctcat cgctgtttct gtcaccactt ggttcacagg
     7921 tggtggctag ccaccaagct gcattgcaaa ccagctcgct attttttatg attccgatgt
     7981 cgtttggcat tgcaaccacc attatgatcg gacaaacact tgggcagaaa aatgtggagg
     8041 gggcgaaaat cttaagttac cacgcactga ttaccgcaac cgctttagct attattgccg
     8101 ccatcattat tattttgttg aaaaatttta tcccttttgc gtttaccaac aacccgattt
     8161 cgattgcgat taccgctcat ttattgattt ttgcggcggt ttatcaaata ccggatgcca
     8221 ttcaagcggt agcaaacggc attctgcgtg gttataaaca tacccaaccg attttatacg
     8281 ttactctgtt ttgttattgg ggggtgagta tgccgctggg ctacattttg gcaagaaccg
     8341 attggattgt acaaccaatg gctgctcaag gtttttggtt agtcttttgt atcggcttaa
     8401 gtttggctgg tggcttgtta ttatggcaaa tgcgaaaaat acaagcggtg ccaaaagcac
     8461 aattaattgc aaaattagaa cggattaaat aacagattct ctgctatgga acatagcagt
     8521 gctttttact tttagaagat aactttgctg atgaaaaacc taaatcctat ccaactacct
     8581 ttgaataaat ccgttttaat cgaagcgtcg gcaggtacag ggaaaacctt taccattgcc
     8641 aacctgtatt tgcgtttatt gctcggcatt ggttgcaacc cgttaacggt ggaacaaatt
     8701 ttggtggtga catttaccaa agcagcaacc gaagagttga aaaaccgtat ccgtaaaaat
     8761 attcagcaat gtgccgactt tttaaaagat caagctgatg gtttggaagt ggaaagtaca
     8821 aaatcctatc gaaacaacct tgattttttg gcacagattt atccgctcat tccgaatatt
     8881 cacgaagcat tgttacgttt aagtattgcc gaacgggaaa ttgataccgc cagcgtgttt
     8941 accattcacg gcttttgtca aaaaatgctg gttcagtttg cgtttgagtc gggcgtgcgg
     9001 tttgacttgg atttacagcc aaaccaaagt gatctactta aaaaattaag tgaagaagtc
     9061 tggcgagagc aattctatcc gcaagattta gccatcactt atgcggttgc cgaacaactt
     9121 ggtacgccgg aatatgccct caatgcggta aggcgttatt tatctactga attacctgaa
     9181 ccaaatgcct ctttaaatca agatattgcc gagcatttgg ctcaatatca gcagtttatt
     9241 aatgaggtga aatcctattg gaaaacgcat tcagatgaac tggttgaaac tatccgccac
     9301 gatttaaagc ataaaattac gctgcacgga ggttggtatc gagcgaccac ttttgaagcc
     9361 ggtgtggaag tgatgaacca gtgggctaat tcgcaatata accatttccc ggcagggctt
     9421 tatttattca ctacagagaa aattgcggct ggcacgaaaa agagctgtga gccgatagat
     9481 tcccctcatc ttgcaaaaaa tcaggctttt ttgaccgctt acaaggaacg ttttgaaaac
     9541 agcttgtcag caatgttgca gtatcaattt ttaattgctc tcaaagcaaa attggcagag
     9601 tacaaacaat cgcacccaca acgcagtttt gatgatttac tcacaatgct aaaccaagcg
     9661 ttgaaaaatc tacacacggg cgaagccttg tctcagaaaa ttcgggcgat gtatcctttt
     9721 gcgatgatcg atgagttcca agataccgat ttaacccaat atgaaatttt cagccaaatt
     9781 tttatggcag agaatgcaga gaacggcttt attatgattg gcgacccgaa acagtcgatc
     9841 tataaattcc gtggggcgga tattttcacg tatttgcgag cagcaaagca ggctgatgaa
     9901 caattcacat tgggcaaaaa ctggcgttcg ttgcctgaag tggtgcaagc ggtcaataat
     9961 ttgttttctt ttgcaaatga taactctccg tttctttatg aggatattgg ctttcaccca
    10021 gtggaggctg atagccaaaa aggtcagtta gttagtgagc aagggcattt tgtaggctac
    10081 atcacggaaa acagtaaatt agatgatttg gctgaaatgt gtgcttatca aattcatcaa
    10141 cagcttgagg cgatggctca aggcaaattc ggagttgaaa aaaacggcga atttcaatcc
    10201 tttgctgcaa aagatattgc gattttagtc cgaaaaggtt cacaagctaa gcgaatcaaa
    10261 aaggcgttgg caaaacgtaa tatcaaatcc gtctatttag ctgaatcagc ttctgtattt
    10321 ggttcggaaa ttgcaacaga attgtgttgg ttattgcaag ccgcactgaa tccgtatcat
    10381 tacaaggcat tattatcggc gttaggctca accttgtggg gcttaacggc ggcagagatc
    10441 caccgctata aggaaaatga gcaactttgg gatctgaaag tcaatcagtt tgtggaatac
    10501 agcaacattt ggaaagagca gggcgttctg ccgatgttgc ataaaatcta cttggagcag
    10561 ggcattatcg aacgtttgaa aggtttggaa aatgcggatc gcttgattac ggatttattg
    10621 catttaaccg aaattttgca ggaaacctcg caagcgttag agtctgaagc tgaactgttg
    10681 cggtggtatg aaaagcaagt taattcgccc gattttaatg acgagcaaca acttcggttg
    10741 gaaagtgaag aggagctaat caaaattgtg acgattcacg gctcgaaagg cttgcaatat
    10801 ccaattgttt ggttaccttt tgtttcacaa aaaagtgagg gagacacgca gacagcatta
    10861 agtttatatc gcaattctca aggtgcgctg gattggtgtt taggctcaga agaggcaagt
    10921 ttttcgctac agtatgaggc ttatgcggaa gatttacgtt tgctgtatgt ggcattaacc
    10981 cgcgcggaat ctcagcttaa cttagtgttg ccgaaagaga tttccggctg gtctccactc
    11041 cattacttgt tagcagacgg ggaaatacct acaggaaaag tgcctgtttc agattatttt
    11101 agccaaaaac ggattgctgc taatttggtt gaagtagccg gggaattacc tttctcactg
    11161 tgggaaggag aacaatcttc agacgattac caagcaaaaa cctttagtgg caatatccgt
    11221 gtggttggtc aaattaccag ctttacttca ctttatgccc aacacgagcg ttttagtgaa
    11281 gggaaatcag aaagggtaat ggatttagcc caagatcgag atgtacatat agaaaccatt
    11341 gaggcaggtt attcaagtga aactgccgct ttctcgccat tccaattccc acacggtatt
    11401 aaagtgggca cggtgttaca tcactttttt gagcattgcc aatttaacga acaaattgat
    11461 agagaggctg ttgctaaagt atgcgagcaa ttagggctaa gcgaggaatg gattgaacca
    11521 acagcattat ggtttgagcg tattctaact acccctttgg cagaggcaaa tttctgctta
    11581 aaagcgattg atgaaaccaa acgattaaac gagtggcaat tctatttacg cttaaaaaat
    11641 gataaagcct tacatcaatt aaatgcgtta ttaaagcaac atagcccttt agcaaaaact
    11701 ttacctgaat tgcaattacc gcaattagaa ggttttgtgc gaggctttgt agattgtatc
    11761 gtgcaagtgg aggagaaatt ctacttaatt gattacaaat cgaatttctt aggctatctg
    11821 ccacaaaatt atgcgaaaga acacattcaa cgggaaatgg gcagacaacg ctatgatttg
    11881 caatatctgc tttacactct cgccttacac cgttatctaa ctgcccgttt aggtgaaaaa
    11941 tacgattatg aacgagactt cggcggagtg gcttacttgt tcttaagggc aatgaacggc
    12001 gaaaatgata acggcgttta ttttgacaag ccgactaagc agttgattga agaaatggat
    12061 cggttgtttg ggtaaatatg tgtaggggcg aaaatttttc gcccctttat gggtaattaa
    12121 ctcgcttgct cataccaaat cttattgata taaagcaaca ctttaccaag cggagtttct
    12181 accctaattt catcatcaac ctgtttgccg attaaggctc gggcgacagg ggagtcaatc
    12241 gaaatccagt tttttgctgg gtcgaactca tcgcaaccaa caatacggta ctgttttgcc
    12301 tcgccctctt cattttctaa ttccacccaa gcgccgaaaa agactttgcc ttcttgttta
    12361 gggtgataat caacaatttg tagcacctct agccgcttag ataaaaaacg cacacggcgg
    12421 tcgatttctc tcaaacggcg tttgccgtaa atatattcgg cattttcgct tcgatcaccc
    12481 aatgcggcag catcagacac cgcttgagtc actttcgggc gttcttcccg ccatagaaat
    12541 tttaattcct gatctaaggc ttgccagcct gaacgggtaa tgtaatttga tttagccata
    12601 gttatttaat tgtttatgga gcaaatagtg atttgcccct acgaagtttc tattttgaaa
    12661 gcaataaatt cgctactgtt cttattccca gtccggttgc tcctgccgcc cataaatcgc
    12721 tcgccgtttt gcggaatgtg gcagaggcgt caatatgtag ccagttttgc tggtagtttt
    12781 ctacaaagta agataaaaat gcggtggcgg tacttgcccc agcccccacc ggcacagagc
    12841 cgatattggc gatgtctgca aaggaagaag caatttgccc acggtgaaat tcttcaaacg
    12901 gtaaacgcca gaacggttca ttttccgttt tggcagactt aaatagtgct tcggttaggt
    12961 tgtcgtccat tgaaagcacc gagtggtagt cattgccgac tgcgactttt gctgctcctg
    13021 tgagggttgc agcatcaata ataaattgtg gattttgctt gcacgcatca attaaaccat
    13081 cggcaagcac taaacgccct tcagcatcgg tgtttaaaac ttcaacagtt acgccattgc
    13141 ggtaagtgat aatatcgcct aatttgaacg cattgtggct gaccatattt tccgcacagc
    13201 agaggtagag tttcactcgt ttattcaagc ctcgagcaat ggcaaaagca agtgagccgg
    13261 ttaaaagtgc cgctccgccc atatcggttc gcattgtgct catactatcg cttggtttta
    13321 gactataacc gccggtgtca aaggtaatgc ctttgccgac caagcaagcc agtacctgtg
    13381 cgttttcatc gttggttggg ttgaagtcta atactaagaa tgcaggcgga ttggcagagc
    13441 cttttccaac tgtccaaatg ccgtgatagc cttgttcttg taatgcttcg ccacgaatga
    13501 tctggtaaga aaccgtattc ggttttgcca ttttttggat aaactcgacc gcttgttcgg
    13561 ctaatttttc aggggttaat ttatctgatg gcttattaat taattcacgc acgaaagtgg
    13621 cacattttac acggttatca aattcggctt gctgttcgtc taagtctggg tattgtaccg
    13681 agccttgatt ttttaccgaa tgaaatcctt gatgaaacgc ccagcaggat tctaaatccc
    13741 aactatcgcc ggtcagctcc acggttgaaa taccttgatt tttaattttg cgggcggctt
    13801 tttgaatagt ggctaaatcg ttgttcggat tatgtacggt aaaccccgca tcatttgcgg
    13861 aaagaatagc atttttaccc caaatttcag gggcgttttg agtagaaagt gaaattttca
    13921 ttgctgtcat tatcggtttc cttagaaaat ggattttgct ttatcctaac aaacttttgc
    13981 aaagttttat acaaaaatga ccgcttgcag agcatagcct tgacaagcgg tcattgtttc
    14041 gtgtttttac aacctgatta aggacggatt atttgccgtc ccaagcttta attgcattgt
    14101 ggcggatttt aacacggctt tcagcattgc ctttgtagta atctttatct aaaccgcctt
    14161 cccagtcgcc atagttcggg ttcggtaaca taatgaattt tttaccgaat tgttggctgt
    14221 tttcggctac gaatgcacgg cgttccgcgt ttgatttacg gtaagtcgca tcgccgaaat
    14281 cgtttaagtt gtcgccaata tagagcacga tttcatagcc ttgtttttcg atttcttcaa
    14341 agcgaggggt tttgttcgat ttgtcttttt tcaacaagag tgtttgttcg ctcacaccac
    14401 taaagcctaa tttgttcata tcatcaatag taccggcttt ttcactgctg tctttacggt
    14461 ttgaaacata gaacatagtt cctttgtggc tattaacgta gttgttaaat tccactgcac
    14521 cggcaatcgc tccggtttgg cgggcattta cccaacgagt ccagctttca ccatcaaacg
    14581 gcttgtggtt tttaacctgc cagccggcat aaaggctgtt gtccaccata gtttcatcta
    14641 aatctactac cacggctttt ttcttgcctt ttttcacttt ggcttggtcg aatgccactt
    14701 tcgctgcatt gaatgcttga tgggcaagag cttggtattc gccggattgt tgcatccagt
    14761 ttatccctaa tgtagcttgc tcttgcaaca ccatttgatg atgctcatcg ctgtttttat
    14821 gcgtgtgcga acaagcagtt aatacaaata aagctgtgag gctcatagca gaaagtttga
    14881 tgtttttcat acgtatatcc ttagttttat tgttgataaa cagagcgatt atacctaaaa
    14941 agagttgctt atggctaata gtttattgct cctattcact tttaaagcaa actaaggtag
    15001 cctagtaacc tttttataac aattacacag aggctatatg caaacattat taaaatttac
    15061 acaatttatc agcaaaacct tcgccatttg ggtggtgttt tttgctttta tcgcagcaca
    15121 atttccggaa acctttaagc aattcctgcc ttggattcct tatctgttag ggattgtgat
    15181 gttgggaatg gggttaacct taactttcaa agattttgcc gaggtaacga aaaatccgaa
    15241 atcagtgatt atcggcgtga tcgctcaatt cgtggtatgc cgagtgtagc ttatggattg
    15301 gcaaaagcat ttaacttacc cgcagattta gccattggtg tgattttagt tggttgttgc
    15361 ccgggcggta cttcttcaaa cgtgatgacc tatttggctc gtggcaacac agcgttatcg
    15421 gtagcttgta ccactatttc aactttactt gccccagtgc tcacgccggc aattttctat
    15481 ttatttgcca gccaatggtt agaaattaat gcaacggcaa tgtttatttc ggtgttgcaa
    15541 atggtgctat tgccaatttt tgtgggcgtg gtgattcgta ctattttcaa acaaaaagta
    15601 gagcaattta gccaaacaat gccattaatt tcagtgattg caatcgtatt aattgtcacg
    15661 gcggtggtgt cagttagccg agatcgtatt attgaatccg gtttattaat ttttgcggta
    15721 gtagcattac acaacggttt aggttattta atcggttatt tggcgagccg tttattaaaa
    15781 ttaccgattg cagacagcaa agcggtggcg attgaagtgg ggatgcaaaa ctcaggattg
    15841 ggggcagcac ttgccgcatt acactttaaa gccaacccta tcattgcggt gccaagtgcg
    15901 gtatttagct tctggcacaa tatttcaggc ccgattttgg cgatgatttt cgctaaaatg
    15961 aaaaacgata acgaaaaata aaattcgttt taatacagac gttttaatgt tttaataaag
    16021 aatagacaaa cggttaaatt tttctaattt tttgcaaaat gttcagaaaa tttaaccgct
    16081 tgttgtttag aatttagaac attcttggtc cacgtgcagt gctgattttt tcatcatccg
    16141 ctctttgggt aacaatcact tgatcaccct catttaagcc tgctaatact tcggtatttt
    16201 gcgtatccga aatgccaatt tggatttttc tttcaaccga ctgctcattt tccagcacca
    16261 acacataatt ctctttacca cgttttttga ttacgctggt gggtacaact aaaacctgtt
    16321 cccgtttatc aatagtaatt tgggcttgag tagtcattcc gatgcgtaaa ctgttatcgg
    16381 cattatcaac ggaaacatta gcgtagtaat aaaccgcttc ggtattaccc gattgttcgg
    16441 tgtagttatt atcggtcagc gtggtgagtg ccggatcaac tgaatcaatg gtagcactat
    16501 attctcgatt tggctcagca agggtggaaa acttaacttt ttgcccgact ttaacctgtg
    16561 caatatcgcc ctccgaaatt tcaagtttaa tcagcatctt agataaatct gctacctgta
    16621 caatagttgg cgaagtttga ttggaattga ccgtttgccc gacagaaacc ggtacagaca
    16681 caatcacgcc gtccattggc gagataattt gcgtataagt taagttggtt ttagcatcgt
    16741 ttaccgaaat ttcagccgat tttaattgtg ctttaatgtt atccaccgtg gctttggctg
    16801 aagctaaggt atttttagcc gtttcaagat cgtttaaaga ggcactattt gcccgataga
    16861 gtttatttaa gcggttgtag ttcgattgag ctacttcaag tgcaacctgt gcagaggtga
    16921 gctgtgcttt ataagaagaa agccgggctt gagcggtgct taaggtgttg ctctggttgc
    16981 tggaatcaat atctgcaatt aaatctcctt gcttcaccga ttgcccgagc tcgacataga
    17041 gtttttggat tttgcccgaa acttgagccc ccacttcggt gcgtttattc gctcgaaccg
    17101 aaccggttgc cagcacattt ttgttaattt ctgcccgtgt gacagcttgg gtaatgtaat
    17161 ggatttgcgg tttattggtt tgttgctgat aataaaaata gccgcctatt ccgagagcgg
    17221 caattagcac aagagtggct aatgttttac gtttcatact gttccttgat gatatacaag
    17281 cggtcaaatt tagacaaaaa tttgcaaaaa agttgctctc aactaaacaa tgccaaatgt
    17341 ggctcgaaga tagcttgcca ccgcttcatc tttgttaaag ccaatcactt cattattcgg
    17401 taatacggct tttaaacgtg gatcggcatt acccattaaa caacctttgc cggctaaact
    17461 tagcatttcc acatcattca taccgtcacc aaaagcgata caatcggcta aggtgtagcc
    17521 ccgtaatttg accaattcgg cgagagcatt ggctttacac actccttttg ccatcacttc
    17581 aaggcaaagt agggcagagt aggtcatttg cacacggtcg ccataggtat cccgaatata
    17641 atgttcaatc ggtactaaag cttcagcagt tttgccgata aaaaacactt tctcggtatt
    17701 tcgaccgtga tgatgagcaa aatttactac ttgatagcta aatcctgaat cttgatggaa
    17761 tttttttaac tctgcaatct ctttattgat aaaccattca tcgccttgat agctgtttaa
    17821 gcacacattt tggtcgtcaa attcaatgtt cattaattca aaggcaagat cttccggtaa
    17881 ataatggttg agaatttttt taccctctaa attgttggct cttgccccgt ttgaagtgac
    17941 taacattgca tttttaacgt ttactttccc aataatctga gacacatctg gatgattgcg
    18001 accggtggcg aaaaaaatat caacaccttt ttttgatagt tgttctaatg tatcaatagt
    18061 gaaatcacca attttatgat tggcatttaa tagtgtgcca tcaagatcgg aaataattgc
    18121 acgaaatggg agagtcataa t
//
LOCUS       DANLYI010000029        18013 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.029, whole genome shotgun sequence.
ACCESSION   DANLYI010000029 DANLYI010000000
VERSION     DANLYI010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 18013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18013
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.029"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            <1..235
                     /locus_tag="PXD81_000746"
     CDS             <1..235
                     /locus_tag="PXD81_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HDL5536798.1"
                     /translation="VTIGANNDQLITFNYIFAQSELRLSTLVAILFGLGLILGWLVTG
                     IFYLKIKFQNVALNRRVKRQAQQITELTTPKAE"
     gene            239..1435
                     /gene="lapB"
                     /locus_tag="PXD81_000747"
     CDS             239..1435
                     /gene="lapB"
                     /locus_tag="PXD81_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HDL5536799.1"
                     /translation="MLELLFLLLPIAALYGWYMGQRSAKKDQETASNKFSREYVTGLN
                     FLLSNQQEKAVDLFLSMLQKQESENHIASESQFEAELTLGNLFRSRGEVDRALRIHQT
                     LVNSPHYSFDQKLLAKQQLAKDFMVAGFYDRAENYYISLVDEPEFAKYSLSQLAVIYQ
                     KTREWKKAINVTEKRLRIEPDADKIPLSHFYCEYAQGVKFDDKAAFIESLNKALEYYP
                     QSTRASIILGDFYLEEGEYLRALSYFEQILVQDPDYISEVLGRIKQSYMALEDLNGYE
                     LFLIRANRVKHNSSVDIALTEFIEEKDGINAAHSKLYQQLSTYPNLITFHRFIRYQAD
                     FAEEGNGKESLVLLHNMVGNQIKRSFQYRCLNCGYQSYRLMWQCPSCNQWEKIKPVQS
                     IEGIIQ"
     gene            1459..2151
                     /gene="pyrF"
                     /locus_tag="PXD81_000748"
     CDS             1459..2151
                     /gene="pyrF"
                     /locus_tag="PXD81_000748"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HDL5536800.1"
                     /translation="MNNKVIVALDYETEREALEFVDLVDPSLCRLKVGKEMFTTLGTN
                     FVKQLHERKFDVFLDLKYHDIPNTVARAVRSAADLGVWMVDLHASGGLAMMEEAKKIL
                     EPYGKEAPLLIAVTVLTSMEDLDLLQIGINASPMEQVIRLAHLAQRAGLDGVVCSPQE
                     VEVLRTHCGQDFKLVTPGIRPKGTDFGDQRRVMTPKQAVETGSDFLVIGRPITQAQDP
                     LAVLKSINQSIS"
     gene            2234..2545
                     /gene="yciH"
                     /locus_tag="PXD81_000749"
     CDS             2234..2545
                     /gene="yciH"
                     /locus_tag="PXD81_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005597129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HDL5536801.1"
                     /translation="MTLVYSTETGRIKSEVIKEERPKGDGIVRIQRQTSGRKGKGVCL
                     ITGLDLEDKALNSLASELKRKLGCGGSFKDGVIEIQGDNRGFIKQILEQKGFKVKLAG
                     G"
     gene            2648..4810
                     /locus_tag="PXD81_000750"
     CDS             2648..4810
                     /locus_tag="PXD81_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HDL5536802.1"
                     /translation="MWKKTLIAVFLASQYAFAVGTGKEPSKSYTETELKQLKNDWLAI
                     YQKELQYAENLRLKQRENFQQINYLADAAISQKNLSLSTEQIIQRLLLTLAGYPLEMK
                     AEWKLLNAKITLKQATESEVKAFISKYPDSLYQRQLQNLMFELLYREQKFPELLEYAQ
                     NVKQSSLADQCRLFSARYELAASKAQINPEVSQVTEQKAENAELLALVQEFDRFWLAT
                     PKLTSDCANLESYWRDQGLKTDEKMRLKAVELIKQNANAELANLAANVNNENLKIWLS
                     SVEKVANNPADLQNFIENQPLDSPFYADNKAIIQQLFAKYVRTLSENMENPSFQQYQT
                     WAEKYQLSSEEINGWKNAFITRFFDNPEPTFQFWRDEQIKLLKTDNLTERRLRMAIWQ
                     KADLNEWLVLLSQQAKEKAEWRYWAAKEEKNEAKRKVDFESLAKERGFYAMLAAQQLG
                     IVYQIPMLEVPELTQAQLELYANQLARIQELRELGQFDQARKIWIDWIKNIKPEAGEN
                     TPTKIQLALIKYAKDKNWYDLAVEGTIQTKAFDYLDLRLPNAYSDWFDLHLQDKKISK
                     TFAQAIARQESAWNAQAQSHANARGLMQLLPTTAQKTAKDNGLTFKDGNDLFQPFNNI
                     MLGTTHLMELNEKYPNNRILIASAYNAGAGRVEQWLKRSNGQLAMDEFIASIPFYETR
                     GYVQNVLAYDYYYQMLYSDINDKNGLKMFYQEELTRKY"
     gene            4859..4935
                     /locus_tag="PXD81_000751"
     tRNA            4859..4935
                     /locus_tag="PXD81_000751"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4893..4895,aa:Met,seq:cat)
     gene            5162..5623
                     /gene="rimP"
                     /locus_tag="PXD81_000752"
     CDS             5162..5623
                     /gene="rimP"
                     /locus_tag="PXD81_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018650956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HDL5536803.1"
                     /translation="MATLEQKLEDLVLDSIEALGFELVGIECQRAGRFLTVRLYIDKE
                     GGVTIDDCSDVSRQVSAIFDVEDPIADKYNLEVSSPGLDRPLFTVAHYERFIGQDVVI
                     HLRIPMFDRRKWQGKIKQVEGDFITLTVENEDRQFAFGNIQKANLVPVFNF"
     gene            5644..7140
                     /gene="nusA"
                     /locus_tag="PXD81_000753"
     CDS             5644..7140
                     /gene="nusA"
                     /locus_tag="PXD81_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005614874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HDL5536804.1"
                     /translation="MSKEILLAAEAVSNEKQLPKEAIFEALETALTIATKKSRDLDID
                     VRVVVDRKTGDFQTFRRWAVVENVNNMTREISLEAAQFEDPNVQLGDFIEDEVESIEF
                     GRVAIQTFGQVVKTKIREAEKTKIIDEFRSDLGKIILATVKKVTRDNIFLELQGKSED
                     IKGEAVIVREDMIPRENFRPGDRVRGVLYEIKPESKGPQLFVTRAKPVMLEELFKLEV
                     PEIGEGVIEIKGSARDVGSRAKIAVKTHDKRIDPVGACVGMRGARVQAISNELGGERV
                     DIVLWDENPAQFVINAMAPADVSSIVINDETHSMDIAVEAKNLAQAIGRNGQNVRLAT
                     QLTGWALNVMTVEELTKKSEEEDGKVINLLTSALEIDEEFAQVLIEEGFTSLEAIAYV
                     PVDTLTAIDGLEDEDLVEELQSRAKNAITKKALEEEKLLKSAQIEEKLLTLEGMDRHI
                     AFKLAEKQITTLEELAEQGVDDLADIEELSEEKAGELIMAARQICWFN"
     gene            7125..9692
                     /gene="infB"
                     /locus_tag="PXD81_000754"
     CDS             7125..9692
                     /gene="infB"
                     /locus_tag="PXD81_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HDL5536805.1"
                     /translation="MLVQLSKEDNKMTEEKKTLSLGGARKASKVNTTTTSGKVKAVEV
                     KEKKPKIDAKALQEKAAKEAAAAKARAEKEAAEKLAKEKAEAEAKEKAAKKAAESTAS
                     KPISVPVMPSSKAKEEKPKAQQVQPKQEKAVDPEKEAKRKEEAELRRKQEELAQQKAE
                     MEAKRAAENARRLAEIAREEAEDNGNEDFDDERFTSSYAREAERDNDRRSEGNRGRNG
                     KGNVAKAKKGGREEDKNERSADRRNHKEVKGKGKQGKKGAALQQAFTKPAQVVKADVV
                     IGETITVAELANKMAVKATEIIKMMMKMGEMVTINQVIDQETAQLVAEELGHKVILRK
                     ENELEEAVLEDRDTHAEKVTRAPVVTIMGHVDHGKTSLLDYIRKAKVAAGEAGGITQH
                     IGAYHVETDDGKMITFLDTPGHAAFTSMRARGAKATDIVVLVVAADDGVMPQTIEAIQ
                     HAKAAGAPLVVAVNKIDKPEANPDRVEQELLQHEVISEKFGGDVQFVPVSAKKGMGID
                     DLLEAIILQSEVLELTAVKDGMASGVVIESYLDKGRGPVATILVQSGTLNKGDIVLCG
                     FEYGRVRAMRDENGKDVESAGPSIPVEVLGLSGVPAAGDEATVVRDEKKAREVALYRQ
                     GKFREVKLARQQKAKLENMFSNMAAGDVAELNIIVKADVQGSVEAISQSLMELSTDEV
                     KVKIVGSGVGGITETDATLAAASNAIMVGFNVRADASARRVIENENIDLRYYSIIYEL
                     LNDVKAAMSGMLQPEFKQEIIGLAEVRDVFRHPKFGAIAGCMVTEGIVKRNNPIRVLR
                     DNVVIFEGELESLRRFKDDVSEVRNGMECGIGVKNYNDVKVGDQIEVFEVVEVKRTI"
     gene            9777..10157
                     /gene="rbfA"
                     /locus_tag="PXD81_000755"
     CDS             9777..10157
                     /gene="rbfA"
                     /locus_tag="PXD81_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HDL5536806.1"
                     /translation="MSREFKRSDRVAQELQKEVAVILQREVKDPRIGMVTVSDVEVSR
                     DLAYAKIFVTFLFDNDAEAIKQGMQGLEKASPYIRTLLGKAMRLRIVPELRFIYDESL
                     VEGMRMSNLVSSVIKNDEEKHKED"
     gene            10160..11083
                     /gene="truB"
                     /locus_tag="PXD81_000756"
     CDS             10160..11083
                     /gene="truB"
                     /locus_tag="PXD81_000756"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HDL5536807.1"
                     /translation="MSRPRKRGRDIHGVFLLDKPQGMSSNDILQKVKRIFQANKAGHT
                     GALDPLATGMLPICLGEATKFSQFLLDSDKRYLVTAKLGERTDTSDAEGQVVETKAVA
                     VTKSQILAALEKFRGDILQVPTMFSALKHNGKPLYEYARAGITVEREARPITIFELNF
                     IEYKAPFLTLEVHCSKGTYIRTLVDDLGEELGCGAHVTMLRRTAVADYPIEKMMSYAD
                     LQNLAENQPLAELDQHLLPMDTAVSALAKINLSSEQTKAVGFGQRIRFDNLNQLYGLV
                     RLYSEDEQFLGVAEITNDTVIRPNRMINLTE"
     gene            11086..12166
                     /locus_tag="PXD81_000757"
                     /pseudo
     CDS             11086..12166
                     /locus_tag="PXD81_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252783.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase"
     gene            complement(12204..12728)
                     /gene="tadA"
                     /locus_tag="PXD81_000758"
     CDS             complement(12204..12728)
                     /gene="tadA"
                     /locus_tag="PXD81_000758"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HDL5536808.1"
                     /translation="MFIKPNQTSCPLQISEQDIAFMQYALDLADLAEAKGEIPVGAVL
                     VDKNQQIIGRGWNQTIQLCDPSAHAEMQAIRQAGQTLGNYRLLDCTLYVTLEPCPMCA
                     GAILHSRIKRLVFGASDYKTGAVGSRYHLFEDYKMNHFLEVHGNVLGRECSQKISDFF
                     KQRRLQKKGDALIK"
     gene            complement(12810..14618)
                     /locus_tag="PXD81_000759"
     CDS             complement(12810..14618)
                     /locus_tag="PXD81_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005618868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDL5536809.1"
                     /translation="MTTESLTFADLGLPQPILDAVNEMGFVNPSPIQQECIPHLLAGR
                     DVLGMAQTGSGKTAAFSLPLLAQIDPTQRHPQMLVMAPTRELAIQVADACEQFAKNMK
                     GIRVVTVYGGQRYDIQLRALKQGSQVVVGTPGRILDHIRRGTLDLSELKFIVLDEADE
                     MLRMGFIDDVETVMAELPETHQTALFSATMPEPIRRITRRFMKDPQEVKIKATERSAP
                     DIEQSYWLVNGFRKNDALLRFLEVEEFDAAIIFTRTKTGTIDITELCERNGYRTAALN
                     GDMTQQAREQTLDKLRSGRLDILVATDVAARGIDIERISLVINYDIPLEEESYVHRIG
                     RTGRAGRSGRALLFVEPRERRLLRNIENLIKKPINEVAIPNHEILMEKRREKFKARIS
                     KQLEHHDLEKYRELLEDLFTADQDHEELAAAMMMLLQEKQKLILPPDPVVRAARSERG
                     RDRNERNDRRNGREHRENNGVAMDLYRIELGREDGVEVRHIVGAIANEGDISSRYIGH
                     IKLYDKYSTIELPQGMPNHLVQHFAHKARVLSKPMQMTLLGSANSASNANPFEGRGRR
                     GNDRNDRGGRRDGGFNKDRKGGFKEKRFSEKRGRRD"
     gene            complement(14820..15743)
                     /gene="nlpI"
                     /locus_tag="PXD81_000760"
     CDS             complement(14820..15743)
                     /gene="nlpI"
                     /locus_tag="PXD81_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HDL5536810.1"
                     /translation="MVQLFKLFRIRFLLLNLISVLMLSGCTSRYADLVSIKNLALAEL
                     NPQLRFEQEAMVVRLSQVLEEAPLTPSEQADLYFERGVIYDSLGLWSLARYDFAQAIS
                     LNQRMAAAYNYMGLYLLLEGDYDSSIDAFNAVLELDREYSYTYLNRGLAFYYSGRYSE
                     AQRDLLHFYEEDKKDPYRALWLYFNELEFTPTEAKNNLQKRANNLSSDYWGTNIVNYF
                     LGKLNLAQVRAIMERDAKENSSQYAEILTETYFYLAKQQLKLDHKDEAISLFRLALAN
                     QVFNFVEYRFALFELSQIRGMTQTTPVVDKE"
     gene            complement(15824..17974)
                     /gene="pnp"
                     /locus_tag="PXD81_000761"
     CDS             complement(15824..17974)
                     /gene="pnp"
                     /locus_tag="PXD81_000761"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HDL5536811.1"
                     /translation="MTPIVKQFKYGQHTVTLETGAIARQATAAVMASMDDTTVFVTVV
                     AKKDVKEGQDFFPLTVDYQERTYAAGRIPGGFFKREGRPSEGETLIARLIDRPVRPLF
                     PEGFFNEIQVIATVVSVNPQISPDLVAMIGASAALSLSGVPFNGPIGAARVGFINDQF
                     VLNPTTSEQKISRLDLVVSGTDKAVLMVESEADILTEEQMLAAVVFGHEQQQVVIENI
                     KEFVKEAGKPRWDWVAPEPNTDLINKVKALAETRLGDAYRIVEKQVRYEQIDAIKAEV
                     IAQLTAEDETVSEGTIIDIITALESQIVRSRIIAGEPRIDGRTVDTVRALDICTSVLP
                     RTHGSALFTRGETQALAVATLGTERDAQIIDELTGEKSDRFLFHYNFPPYSVGETGRI
                     GSPKRREIGHGRLAKRGVLAVMPTAEEFPYVVRVVSEITESNGSSSMASVCGASLALM
                     DAGVPIKAAVAGIAMGLVKEDEKFVVLSDILGDEDHLGDMDFKVAGTRTGVTALQMDI
                     KIEGITPEIMRIALNQAKGARMHILGVMEQAIPAPRADISDYAPRIHTMKIDPKKIKD
                     VIGKGGATIRALTEETNTSIDIDDDGTVKIAATDGNAAKAVMARIEEIVAEVEVNQIY
                     NGKVTRVVDFGAFVSILGGKEGLVHISQITNERVERVADYLTVGQEVQVKVVEIDRQG
                     RIRLTMKDINNTNEANAEETVAENVVETEQENNF"
BASE COUNT         5487 a         3436 c         4046 g         5044 t
ORIGIN      
        1 tgttacgatt ggagccaata atgatcaact tatcaccttt aactatattt ttgctcagag
       61 tgaattacgc ttatcaacgt tagttgcgat tttatttggc ttaggattga ttcttggttg
      121 gttggtaacg ggtatttttt acttaaaaat taagtttcaa aacgttgctt taaatcgtcg
      181 tgtaaagcgc caagctcagc aaataactga attaacgact ccaaaggctg aataatcaat
      241 gcttgaatta ttgttccttt tattgcctat tgcagcacta tatggctggt atatgggaca
      301 acgtagtgca aagaaggatc aagaaaccgc tagtaataag ttctctcgtg agtatgttac
      361 ggggttaaat tttcttttat ctaatcagca ggaaaaggcg gtggaccttt tcctctctat
      421 gttacaaaaa caagaatccg aaaatcatat tgcatctgaa tcccaatttg aagcggagtt
      481 aaccctaggt aatcttttcc gttcaagagg tgaggtagat cgtgccttac gtattcacca
      541 aacattagtg aatagccctc attattcatt tgatcaaaaa ttattagcaa aacagcaatt
      601 agctaaagac tttatggttg caggtttcta tgaccgtgca gaaaattatt acatttcatt
      661 agttgatgaa cctgagtttg ctaaatattc tttatctcag cttgctgtta tttaccaaaa
      721 aaccagagaa tggaaaaaag cgattaatgt tacagaaaaa cggcttcgaa tagagccgga
      781 tgcagataaa ataccgcttt cacattttta ttgtgaatat gcgcaagggg ttaaatttga
      841 tgataaagcg gcatttattg aatcgttaaa taaagcgtta gaatattatc ctcaatccac
      901 acgcgcttct ataatattag gtgatttcta tttagaagag ggagaatatc taagagcatt
      961 aagctatttc gaacagattc ttgtgcaaga ccctgattat attagcgaag tgcttggacg
     1021 aattaagcaa agctatatgg ctcttgagga cttaaatggc tatgagttat ttctcattcg
     1081 agcaaaccga gtaaaacata atagtagtgt agatattgct ttaactgaat ttattgaaga
     1141 aaaagatggt atcaatgctg cacattctaa actataccag cagttgagta cttatccgaa
     1201 tttaattact tttcaccgct ttattcgcta tcaagcagat tttgcggagg aaggaaatgg
     1261 taaggaaagc ttagttttat tacataatat ggtgggaaat cagataaaac gcagcttcca
     1321 atatcgctgt ttaaattgtg gttatcaaag ttatcgctta atgtggcaat gtccttcttg
     1381 taatcaatgg gaaaaaatta agccggtaca aagtatcgaa ggtattattc aataaagaaa
     1441 gaatctgagg aattgaaaat gaacaataaa gtaattgttg cgttagacta tgaaactgaa
     1501 cgtgaagcat tagaatttgt ggatttggtc gatccgtcgc tttgtcgttt aaaagtaggc
     1561 aaagaaatgt ttacaacctt aggcacaaat tttgtcaaac aattacacga acgtaaattt
     1621 gatgtattcc ttgatttaaa atatcatgat attccaaata cggttgctcg agcagtacgt
     1681 tctgctgctg atcttggtgt gtggatggtt gacttgcatg cttctggtgg tttagccatg
     1741 atggaagagg caaagaaaat tttggagcct tatggcaaag aggcaccatt attaattgca
     1801 gtgacggttc taaccagtat ggaagatttg gatttgcttc aaataggtat taatgcttcg
     1861 ccgatggaac aagttattcg tttagcacat ttagcacaac gggctggttt agatggcgtg
     1921 gtatgttctc cacaagaagt tgaggtatta cgtacccatt gtggtcaaga ttttaaatta
     1981 gtgaccccag gaatccggcc aaaaggaaca gattttgggg atcaacgccg agtgatgaca
     2041 cccaaacaag cagtagaaac aggctcagat tttttggtta ttggtcgtcc tattacccaa
     2101 gctcaagatc cgcttgcggt attaaaatct attaaccaat ctatcagtta attcattgca
     2161 tttttaatga aggcggaaaa atttccgcct tttttaatca aacaagcggt tatttttgag
     2221 gaaaaatttg taaatgacat tagtctattc aacggaaaca ggcagaataa agtccgaagt
     2281 aataaaagaa gaacgcccta aaggggatgg tattgtgcgt attcaacgtc aaacaagcgg
     2341 tagaaagggg aaaggtgttt gccttatcac cggtcttgat ttagaggata aagccttaaa
     2401 ttctcttgct tcagagctta aacgtaaatt gggttgtggt ggctcattta aagatggagt
     2461 aattgaaatc cagggcgata atcgaggatt cattaagcaa atcttagaac agaaaggttt
     2521 caaagtgaaa ctggcaggtg gttgagtttt aaacaattat cagctggcag tttttaaata
     2581 acttcatttt atttgaaact tcattcctac ttttctgcct aaaactcacg attaaggaga
     2641 aaaactaatg tggaagaaaa cgttgattgc tgtattttta gcaagccaat atgcttttgc
     2701 ggtaggaacc gggaaagaac catcaaaaag ttatactgaa acagaattaa aacaattaaa
     2761 aaatgattgg ttagcgattt atcaaaaaga gctacagtat gcagaaaatt tgcgtttaaa
     2821 acagagagaa aattttcagc aaattaatta tttggcagat gcagcgatta gtcagaagaa
     2881 tttatcactt tcaacggagc agataattca gcgtttatta ctaaccttag cgggttatcc
     2941 gttggagatg aaagctgaat ggaagctgct aaatgctaaa ataacgttaa aacaagccac
     3001 agaaagtgaa gttaaagcat ttatttcaaa atatcctgat tctttgtatc aaaggcagtt
     3061 acagaacttg atgtttgagc tgctctatcg tgaacagaaa tttccggagt tactggaata
     3121 tgctcaaaat gtaaaacaga gtagtcttgc cgaccaatgc cgtttatttt ctgctcgtta
     3181 tgaactggca gcaagtaaag ctcaaatcaa tccggaagtt tctcaagtaa ctgagcaaaa
     3241 agcagaaaat gcagaactgc ttgctttggt gcaagagttt gatcgctttt ggcttgctac
     3301 gccgaaatta acttctgatt gtgcgaattt agaaagttat tggcgggatc aggggcttaa
     3361 aaccgatgaa aaaatgcgtt tgaaagcggt cgaattaatt aaacaaaatg caaatgcaga
     3421 attggcgaat ttggcggcaa atgtaaataa tgagaatttg aaaatctggc tatcttccgt
     3481 tgaaaaggta gcgaataatc cggcagattt gcaaaatttt atcgaaaatc aaccgcttga
     3541 ttccccattt tatgctgata ataaagcgat tattcagcaa ctctttgcta aatatgtgcg
     3601 aactttatcg gaaaatatgg agaacccgag tttccagcag tatcaaacgt gggcagagaa
     3661 gtatcaactt tccagcgagg aaatcaatgg ctggaaaaat gcttttatca ctcgattttt
     3721 tgataaccca gaaccaacat tccaattttg gcgtgatgag caaatcaagt tgctcaagac
     3781 agacaattta actgaacgcc gtttacgaat ggcaatttgg caaaaagcag atttaaatga
     3841 gtggctggtt ctgctatctc agcaggcgaa agaaaaagca gaatggcgtt attgggctgc
     3901 gaaagaggag aaaaacgaag cgaagcgtaa agttgatttt gaaagcttag ctaaagagag
     3961 aggcttttat gctatgttgg ctgctcaaca attaggcatt gtttaccaaa tcccgatgct
     4021 tgaagtgcct gaattgacac aagcacagct tgaattgtat gcaaaccaat tagctcgtat
     4081 tcaagagttg cgagagctag ggcagtttga ccaggcaaga aaaatatgga ttgactggat
     4141 aaaaaacatc aagccggaag caggtgaaaa tacaccaact aaaatccagt tagccctgat
     4201 aaaatatgcc aaggataaaa attggtatga tttggcggtg gaaggtacaa ttcaaacaaa
     4261 agcctttgat tatcttgatt tgcgattacc gaatgcctat tcggattggt tcgatttaca
     4321 tctgcaagat aaaaaaatca gcaaaacgtt tgctcaggcg attgctcgtc aagaaagtgc
     4381 gtggaatgct caggctcaat cgcacgctaa tgcgagaggg ctaatgcaac tgttaccgac
     4441 aacagcacaa aaaaccgcta aagataacgg tttaaccttc aaagatggca atgatctctt
     4501 tcaaccgttt aacaatatta tgttaggcac aacccattta atggagttaa acgaaaaata
     4561 tccgaataat cggattctga ttgcctctgc ttataatgca ggagcagggc gtgttgagca
     4621 gtggctgaaa cgtagtaacg ggcagttagc aatggatgaa tttattgcca gtatcccgtt
     4681 ttatgaaacc agaggctatg tacaaaatgt gctggcgtat gactactatt atcaaatgct
     4741 ctatagcgat ataaatgata aaaatggatt aaaaatgttc tatcaagagg aattaacaag
     4801 aaaatattaa aaaatgcttg tgtttttgtg tagaaccgct ataatgcacc ccatcagacg
     4861 cggggtggag cagcttggta gctcgtcggg ctcataaccc gaaggtcgtt ggttcaaatc
     4921 cggcccccgc aaccagtttt aagctcacag ctttggttaa agttgtgagt tttgttttat
     4981 atgctgtagc aaatttgcaa aaaatttggc aaaaaagacc gcttgttgtg gttgctacag
     5041 caaaaaacaa accgcttaaa gccccacggt ttcccgtctg gggctttttt gcggaaattg
     5101 gtacagcatt aacgtatcaa agctggttta tccggctttt tttatatttg gagattatga
     5161 cttggcaact ttagaacaaa aattagaaga tttagtgctc gattctattg aagcattagg
     5221 ctttgaatta gtcggcattg aatgccaacg tgcaggccgt tttttaacgg tgcgtttata
     5281 tattgataaa gaaggtggcg tgacgattga tgattgtagc gatgtcagcc gccaagtgag
     5341 tgcgattttt gatgtggaag atccgattgc cgataaatat aacttagagg tttcttcacc
     5401 agggttagat cgtccattat ttaccgttgc ccactatgag cgttttatcg ggcaagatgt
     5461 agtgattcat ttacgcattc cgatgttcga tcgccgtaaa tggcaaggta aaatcaaaca
     5521 agtagaaggt gatttcatca ctttaacggt agaaaatgaa gatcgccagt tcgcatttgg
     5581 caatattcaa aaagcaaatt tagttccggt ttttaatttt taagataaag gtaaactgac
     5641 aaaatgagta aagagatttt attagctgcg gaagcagttt caaacgaaaa gcaattacca
     5701 aaagaggcaa tttttgaagc cttagaaacc gctttaacca ttgcaaccaa aaagagccgt
     5761 gatttagata ttgacgtgcg tgtggtggta gatcgtaaaa ccggtgattt ccaaactttc
     5821 cgtcgttggg ctgtggttga aaatgtgaat aatatgaccc gtgaaatcag cttagaagcg
     5881 gcacaatttg aagatccaaa cgtacaatta ggcgatttta ttgaagatga agtagagtct
     5941 atcgagttcg gtcgtgtggc gattcagact tttggtcagg tagtcaaaac caaaattcgt
     6001 gaagcggaga agacgaaaat cattgatgaa ttccgttcgg atttaggcaa aattattctt
     6061 gccactgtga aaaaagtaac ccgtgataat attttccttg agttacaagg taaatcagaa
     6121 gatatcaaag gcgaagcggt cattgtgcgt gaagatatga tcccacgtga aaacttccgc
     6181 ccgggtgacc gtgtgcgtgg tgtgttatac gaaatcaagc ctgaatcaaa aggacctcag
     6241 cttttcgtta cccgtgctaa accggtgatg ttagaagagc tattcaaatt ggaagtgcct
     6301 gaaattggcg aaggcgtaat tgagatcaaa ggttcagccc gtgatgtcgg ctctcgtgca
     6361 aaaattgccg tgaaaaccca cgataaacgt attgacccgg taggggcttg tgtgggtatg
     6421 cgtggtgctc gtgttcaagc tatcagcaat gaattaggcg gcgagcgtgt ggatatcgta
     6481 ctttgggacg aaaatccggc tcaatttgtg attaatgcaa tggctccggc agatgtaagc
     6541 tctattgtga ttaacgatga aactcactca atggatattg cggttgaagc gaaaaatctg
     6601 gctcaagcta tcggtcgtaa cggtcaaaac gtgcgtttgg cgacacaatt aactggttgg
     6661 gcattaaatg taatgaccgt tgaagagcta acgaagaaga gcgaagaaga agatggtaaa
     6721 gtcatcaatt tattaacttc tgctttagaa attgatgaag aatttgctca agttttaatt
     6781 gaagagggct ttacgtctct tgaagcgatt gcttatgtgc cggttgatac tttaacggcg
     6841 attgacggtt tagaagatga agatttagtc gaagaattac aatctcgtgc caaaaatgcg
     6901 atcacgaaaa aagcgttaga agaagagaag ttattaaaat cagctcaaat tgaagaaaaa
     6961 ttattaacgc ttgaaggtat ggatcgccat atcgcattca aattagctga aaaacagatt
     7021 acgactcttg aagaacttgc agagcaaggt gttgatgatt tagccgatat tgaagaactt
     7081 tcagaagaga aagctggcga gctaattatg gcagcacgcc aaatttgttg gttcaactaa
     7141 gcaaagagga taataaaatg acagaagaga aaaaaacact ttcgcttggt ggcgctcgca
     7201 aagcgagtaa agtgaataca accacaacca gtggtaaagt aaaagcggtt gaagtaaaag
     7261 agaaaaaacc gaaaattgat gcaaaagcat tgcaagaaaa agcagcaaaa gaagctgccg
     7321 cagcgaaagc tagagctgaa aaagaagcag ctgaaaagtt agccaaagaa aaagccgaag
     7381 ctgaagctaa agagaaagct gcaaaaaaag cagcagaatc gaccgctagt aaaccgattt
     7441 ctgtgccggt aatgccaagc agcaaagcaa aagaagaaaa accgaaagca caacaggtac
     7501 agccaaaaca agagaaagcg gttgatcctg aaaaagaagc taaacgcaaa gaagaagctg
     7561 agttacgccg taagcaagag gaattagctc agcaaaaagc ggaaatggag gcaaaacgtg
     7621 ctgcagaaaa tgcccgccgt ttagcagaaa ttgctcgtga agaagcggaa gataacggca
     7681 acgaagattt tgatgatgag cgttttactt cttcttatgc tagagaggct gagcgtgata
     7741 atgaccgccg tagtgaggga aatcgtggtc gtaacggcaa aggcaatgtg gctaaagcga
     7801 aaaaaggcgg acgtgaagag gataaaaacg aacgcagtgc agatcgtcgc aaccataaag
     7861 aagtaaaagg caaaggtaaa caaggtaaga aaggtgcagc tttacaacaa gcctttacca
     7921 aaccagctca agttgttaaa gctgatgtgg tgattggtga aactatcact gtggctgaat
     7981 tggctaataa aatggcagtg aaagcgactg aaatcatcaa aatgatgatg aaaatgggtg
     8041 aaatggtgac gatcaaccaa gttatcgacc aagaaaccgc acaattagtg gcggaagaac
     8101 ttgggcataa agtgattttg cgtaaagaaa atgagttaga agaagcggtt ttagaagatc
     8161 gtgatactca cgcagaaaaa gtaacccgtg caccggtagt gactattatg ggccacgttg
     8221 accacggtaa aacctcgtta cttgactaca ttcgtaaagc caaagtggcg gcaggtgaag
     8281 caggtggtat tacccagcac attggtgcat accacgttga aaccgatgac ggcaagatga
     8341 tcactttctt agataccccg gggcatgcag cctttacctc aatgcgtgcg cgtggtgcga
     8401 aagcaactga catcgttgtt ctcgtcgttg cggctgatga cggtgtaatg ccacaaacta
     8461 ttgaagctat ccagcacgcc aaagcagcgg gtgcgccatt agtggtagcg gtgaataaaa
     8521 ttgataaacc ggaagcgaac cctgaccgtg tagaacaaga gttactacaa cacgaggtta
     8581 tttctgagaa attcggtggc gatgtgcaat tcgtgccggt atctgcgaaa aaaggtatgg
     8641 ggatcgatga tttactagaa gccattattc tgcaatccga agtgcttgaa ttaaccgcag
     8701 taaaagacgg tatggcaagc ggcgtggtaa tcgaatctta cttagataaa ggtcgtggcc
     8761 ctgtggcaac tatcttggtt caatccggta cgctaaacaa aggcgacatt gttctatgtg
     8821 gttttgaata cggacgtgtg cgtgcaatgc gtgatgaaaa cggtaaagat gttgaatcag
     8881 caggtccgtc tattccggta gaagtattag gcttatcagg cgtgcctgct gccggtgatg
     8941 aggcaaccgt cgtgcgtgat gagaagaaag cccgagaagt ggctctttat cgccaaggta
     9001 aattccgtga agtaaaactg gctcgtcagc aaaaagctaa actggaaaat atgttcagta
     9061 atatggcagc cggtgatgtg gcagaattga acatcattgt taaagcagac gtacaaggct
     9121 cggtagaagc gatcagccaa tccttaatgg aactttcaac cgatgaggtg aaagtgaaaa
     9181 ttgttggttc aggtgtaggt ggtattaccg aaactgatgc aaccttagcc gcagcttcta
     9241 atgctattat ggtcggcttt aacgtgcgtg ccgatgcctc tgcccgccgt gtgattgaaa
     9301 acgaaaatat cgatttacgt tactactcca tcatttatga gctattaaat gacgtgaaag
     9361 cagcaatgag cggtatgtta cagcctgaat tcaaacaaga aattatcggc ttggcagaag
     9421 tgcgcgatgt gttccgccac ccgaaatttg gggcgattgc cggttgtatg gtgacagaag
     9481 gtatcgttaa acgtaacaac ccaatccgtg ttctgcgtga taacgtagta atttttgaag
     9541 gcgagttaga atctctccgc cgtttcaaag atgacgtatc tgaggtgcgt aacggtatgg
     9601 aatgtggtat cggcgttaaa aactacaacg acgtaaaagt tggcgaccaa atcgaagtgt
     9661 ttgaagtggt tgaagtaaaa cgtactattt agcctaattt tgtgtagggg cgtattgcat
     9721 acgccctttg tattttttag ggcgtgaagc atcacgcccc tataaaagag aaaaatatgt
     9781 ccagagaatt taaacgtagt gaccgtgtgg cacaggagct acaaaaagaa gtggcggtga
     9841 ttttacaacg tgaggtaaaa gatcctcgca ttggtatggt gactgtttct gatgtggaag
     9901 tatcaagaga tttggcgtat gccaaaattt ttgtgacttt cttatttgat aacgatgctg
     9961 aagctattaa gcaaggtatg caaggcttgg agaaagcaag cccctatatt cgaacccttt
    10021 taggtaaagc aatgcgtttg cgtattgtgc ctgaactgcg ttttatctac gatgaatcgc
    10081 tagtcgaagg tatgcgtatg tctaaccttg tgtctagtgt aatcaaaaat gacgaagaaa
    10141 aacataaaga ggactaattg tgtctagacc tcgcaaacgt ggccgtgata ttcacggcgt
    10201 tttcttatta gataaaccac aaggaatgtc ttcaaacgat attctgcaaa aggtaaagcg
    10261 aattttccaa gcaaataaag ccggacatac cggggcgtta gaccctctcg caaccggaat
    10321 gctgccgatc tgtttaggtg aagcgaccaa attttcgcaa tttttgttgg attcggataa
    10381 acgttatctc gtcaccgcaa aattaggcga gcgaaccgat acttcagatg cggaagggca
    10441 agtggtggaa accaaagcag ttgcagtgac aaaatctcaa attttggctg ctttggagaa
    10501 atttagaggt gatattttgc aagtgccgac aatgttttcg gccttaaaac acaacggcaa
    10561 acccttgtat gaatatgccc gagcagggat tacggtggag cgtgaggctc gcccgattac
    10621 gatttttgaa ctaaatttca ttgaatacaa ggctccattt ttaacccttg aggtgcattg
    10681 ttctaaaggt acttatatcc gaactttggt cgatgattta ggcgaagagt tgggctgtgg
    10741 tgcacacgtt acgatgctac gccgtacagc agtggcagat tatccgattg aaaaaatgat
    10801 gagctatgca gatttgcaaa atttagccga aaatcaaccg cttgcagagt tagaccagca
    10861 cttattgccg atggatacgg cagtttctgc attagcaaaa ataaatttaa gttcagagca
    10921 aactaaagcg gtgggttttg gtcagagaat tagatttgat aatctgaacc aactttatgg
    10981 tttggtacgt ttatattcag aagatgaaca atttctcggt gtggcagaaa tcaccaatga
    11041 tactgtgatt cgtcctaacc gaatgattaa cttaacggaa taagaatgaa atcgataatt
    11101 tttaccttac ctttagcgat tatcacttta gccttagccg gttgttcaag tgataatcaa
    11161 gaaagttatg ccggcttaaa cttagatgct aaatacagta aatctcgctc ggtcagtcat
    11221 tttaatgatt atgtgcattt tctgaaacag aaggcagcag gggcaggagt gtctgcaaaa
    11281 accttaaatg ctcagaaatt cattcattac aacgccagag cggtgcagtt agatcaacag
    11341 caagctgcac gaagacgtga cccgaatgta ccgcctccgc caccaaatcc aagttttaaa
    11401 caaagtatta actcaagcga aggtggatca ggcaagtgaa cgttggtatg aatataatag
    11461 ccagcttacc catgccagcc aaaaatatgg ggtacaaaaa gagtatattt tggcactttg
    11521 gggaatggag agcagctttg ggcgtaacca aggggatttt gatgtacttt cggtgttggc
    11581 aaccctcgcc tttgatggtc gccgtgagaa gttatttacg caagaatttg tgaatgcgat
    11641 gaaaatgctc gatgacggca caattaaacg ctatgaattg cttggatctt gggcaggagc
    11701 gatgggacaa actcaattta tgccaacttc ttacttaacc tatgcagctg atggcaataa
    11761 tgacggtaaa aaagatattt gggagaatga agccgatgca tttgcctcga ttgccaatta
    11821 tctttcaaca gtaggttggg ataatcaact accttggggt gttgaggtta aattagcttc
    11881 accgatggat ttaagtttcg ccggtcttga aaccaacaaa gcgaaaacat tggcggaatg
    11941 gcaagcctta ggtatttatt tagcttatcc gaatggacaa gaatcggcaa aattagctca
    12001 attacacggc aaagaggttt ggttgattcg tccggataaa gaagcgggaa gagcgttttt
    12061 agtttctaat aatttccgta ctttaatgga ttggaataaa tcgaataacc ttggtatcag
    12121 tatcggaaaa tttgccgatc gaattttagc ttcagttggt caataatcga ataaaagcat
    12181 agtagcaaag cctatctatg cttttatttt atcaaagcgt cgcctttctt ctgcaaacga
    12241 cgttgcttaa aaaaatcact gatcttttgg ctacattctc tgcccaatac attgccatgc
    12301 acttctaaga aatggttcat tttataatct tcaaacagat gatagcgtga acctaccgca
    12361 cctgttttat aatcacttgc cccaaaaacc aatcgcttaa tccgactatg taaaattgca
    12421 ccggcacaca ttgggcaagg ctcgagcgta acataaaggg tacaatctaa cagacggtaa
    12481 ttccctaaag tttgtcctgc ttgacgaatt gcctgcattt cagcgtgtgc agatggatca
    12541 cagagttgaa tggtttggtt ccagcctctg ccgataatct gttggttttt atcaaccagt
    12601 actgccccaa ccgggatttc gccttttgct tcggctaaat cagcgagatc aagggcgtat
    12661 tgcataaagg cgatgtcttg ctcggagatc tgaagtggac aagaagtttg atttggtttg
    12721 ataaacatag ttgcaaaaaa ttgagttttt ttgaccgctt gtaaaatgcc ccacgagggt
    12781 ggggcatcgc ttacttcgct tagtgtcgtt tagtcacgac gaccacgttt ttcactgaaa
    12841 cgtttttctt taaagccacc tttgcggtct ttattgaaac ccccgtctcg gcgaccacca
    12901 cgatcattac gatcgttacc acggcgacca cgaccttcaa atggatttgc attacttgca
    12961 ctgtttgcag agccaagtaa tgtcatctgc attggtttgc ttaatacacg tgctttgtga
    13021 gcgaaatgtt gtacgagatg atttggcatt ccttgcggca actcaatggt tgagtattta
    13081 tcataaagtt tgatatgccc gatgtaacgg ctgctgatat ccccctcgtt ggcaatcgct
    13141 ccaacaatat gacgaacttc cacaccgtct tcacgcccca attcaatgcg gtataaatcc
    13201 attgccacac cattgttttc acggtgttca cgaccgttac gacgatcgtt tcgctcattg
    13261 cggtcacgtc cacgttcaga acgggcagca cgcacaaccg gatccggtgg cagaatcagt
    13321 ttttgttttt cttgcagtaa catcatcatc gccgctgcta attcttcgtg gtcttgatcc
    13381 gcagtaaata aatcttccaa caattcacga tatttttcta aatcgtgatg ttctaactgc
    13441 ttagaaatgc gggctttgaa tttctcacgg cgtttttcca ttaaaatttc gtggtttgga
    13501 atcgccactt cattaattgg ttttttgatt aaattttcga tattgcgtaa taagcggcgt
    13561 tcacgaggct caacaaacag taaggcacga cctgaacgac cggcacgacc ggtacgtccg
    13621 atgcggtgaa cataagattc ttcttctaac ggaatatcgt agttaataac caagctgatt
    13681 cgctcaatat caataccacg ggcagcaaca tcggtggcga ctaaaatatc taaacgaccg
    13741 gagcgtaatt tatctaaggt ttgctcacgg gcttgctggg tcatatcgcc gtttaatgcc
    13801 gcagtacggt agccgttgcg ttcacacaat tcggtaatat caatagtacc ggttttggtg
    13861 cgggtaaaga tgatagcagc atcaaattct tctacttcta ggaaacgcaa taacgcatcg
    13921 tttttacgga agccgtttac cagccagtag ctttgttcaa tatccggggc tgaacgctcg
    13981 gttgccttaa tttttacctc ttgcggatct ttcataaaac gacgggtaat acggcgaatc
    14041 ggctccggca tggtggcaga gaagagagca gtttgatgtg tttccggtaa ttcagccatt
    14101 accgtttcaa catcgtcaat aaagcccata cgcagcattt catcggcttc atctaacacg
    14161 atgaatttta attcggataa atctaatgtg ccacggcgga tatggtcaag aatacgccca
    14221 ggtgtaccca ctactacttg tgagccttgt tttaaagcac gtaattgaat gtcgtaacgt
    14281 tgtccaccat aaaccgtcac tacacgaatg cctttcatat tttttgcaaa ctgctcacaa
    14341 gcatcggcta cttgaatcgc aagctcacgg gtgggtgcca tcaccaacat ttgcgggtgg
    14401 cgttgtgttg ggtcaatttg tgccaacagc ggcagcgaga atgcggcggt tttaccgctc
    14461 ccggtttgtg ccataccaag cacatcacga ccggctaata aatgaggaat acattcttgt
    14521 tgaatgggtg atgggtttac aaagcccatt tcatttacag catcaaggat tggttgaggt
    14581 aagcctaaat cggcgaaagt taaagattca gttgtcataa aaaaccattt gtttggaacg
    14641 cacaaagcag aaaatatcga ctatttttct gcacagccat taatgaaaat tatattgttg
    14701 aaatttagga agaaatgaaa taacaaagca agtgatgctt taaaaatccc ctgagtataa
    14761 gcggttaaat ttacaaaatt ttttgcgaaa ttaaccgctt gtattgtcct aatctttccc
    14821 tattctttat caacaacggg agtcgtttga gtcataccac gaatttgtga taactcaaac
    14881 agggcaaaac gatactcaac gaagttaaac acttgatttg caagagcaag acgaaagagg
    14941 ctgattgcct catctttatg atctaatttg agttgttgtt ttgctaagta gaaatacgtt
    15001 tctgtcagta tttctgcata ttgtgagcta ttttccttag cgtcacgttc cattatagca
    15061 cgaacttgag ctaaattgag cttccctaaa aaataattta cgatatttgt tccccagtaa
    15121 tccgaagaaa gattattagc tcgcttttgt aaattatttt tagcttctgt tggtgtaaat
    15181 tccagctcat taaaatagag ccataatgct cgataaggat ctttcttatc ttcttcataa
    15241 aaatggagaa gatcacgctg ggcttcagaa tatctgccgc tataatagaa agccaaacct
    15301 cgattgagat aggtgtagct atattcccga tctaattcca atacggcatt aaacgcatca
    15361 atcgaactgt cgtaatcgcc ttccagcagc agatacagcc ccatatagtt ataagctgcc
    15421 gccatacgtt gattgagcga aatggcttga gcaaaatcat aacgggctaa cgaccataaa
    15481 cctaaactgt cgtaaataac gcctctttca aaatagaggt ctgcttgctc agagggcgtt
    15541 aatggggctt cttccaatac ttggctgagt cttaccacca ttgcctcttg ttcaaagcga
    15601 agttgaggat taagttctgc caatgctaag tttttaattg aaaccaaatc ggcataccga
    15661 ctggtacagc cgcttagcat taaaacagaa attaaattca gtaataagaa acggattcga
    15721 aacaacttaa aaagttgtac cattacagca tatccctcaa ttaaggaaaa ttaaaaactg
    15781 aataacggac actcaaagtc cgttattcgc ctttattatt gaactagaaa ttattttctt
    15841 gttctgtttc taccacattt tcagctacag tttcttctgc atttgcctcg ttggtattat
    15901 tgatgtcttt catcgtcaga cgaatgcgtc cttgacggtc aatttctacc actttcactt
    15961 gtacttcttg accaacggtt aagtagtccg ctacacgctc aacacgttcg ttggtgattt
    16021 gtgaaatgtg gactaaacct tctttgccac ctaagatgga aacgaatgca ccgaagtcca
    16081 ccacacgggt tactttaccg ttgtagattt ggtttacttc cacttcggca acgatctctt
    16141 caatacgagc cattactgct ttcgctgcat tgccgtcagt tgccgcaatt ttcaccgtac
    16201 cgtcatcatc aatgtcgata gaagtattgg tctcttcggt taaagcacga attgttgcac
    16261 cgcctttacc aatcacatct ttgattttct tcggatcgat cttcattgtg tgaatgcgtg
    16321 gggcatagtc agaaatatct gcacgaggtg ccggaatggc ttgttccatt acacctaaaa
    16381 tgtgcattct tgcaccttta gcttggttta aggcaatacg cataatttca ggggtgatcc
    16441 cttcgatttt gatgtccatt tgcagcgcag tcacaccggt acgggttccc gctactttaa
    16501 agtccatatc gcctaagtgg tcttcatcac ctaagatgtc agaaagtacc acgaatttct
    16561 cgtcttcttt cacgagcccc attgcgatac cggcaaccgc tgctttgatt ggcacacctg
    16621 cgtccatcaa cgcaagagac gcaccacaca cagatgccat tgaagaagaa ccgttagatt
    16681 cagtgatttc agacaccaca cgcactacat acgggaactc ttcagcggtt ggcatcacgg
    16741 ctaatacgcc acgttttgct aaacgaccgt gaccgatttc acggcgtttt ggcgagccga
    16801 tacgaccggt ttcgcccaca gagtatggag ggaaattgta gtggaataag aaacggtcag
    16861 atttttcgcc ggtcaattcg tcaatgattt gggcatcacg ctctgtgccc aatgttgcta
    16921 ctgctaatgc ttgggtttcg ccacgggtga aaagagcaga accgtgggtg cgtggtaaca
    16981 cactggtgca aatatccaat gcacgcacgg tatccaccgt acggccgtca atgcgtggtt
    17041 cgcctgcaat aatacggcta cgcacgattt ggctctctaa tgcggtgatg atgtcgatga
    17101 tagtcccttc agaaacagtt tcatcttctg cggtaagttg tgcaatcacc tctgctttaa
    17161 tcgcatcgat ttgctcgtaa cgaacttgtt tttctacgat acgataagca tcgccaaggc
    17221 gtgtttctgc taatgctttt actttgttga ttaaatctgt atttggctct ggtgcaaccc
    17281 aatcccaacg tggtttgccc gcttctttaa caaattcttt gatgttttcg attacaacct
    17341 gttgttgctc gtggccgaac accaccgccg ctaacatttg ctcttcggtt aagatatccg
    17401 cttcagattc caccatcaac acggctttgt ctgtacctga aaccactaaa tctaagcggc
    17461 tgattttttg ctcgctggtg gttgggttta atacgaattg atcgttgata aaaccgacac
    17521 gagccgcacc gattggaccg ttaaacggca cgccggataa tgaaagggca gccgatgcac
    17581 cgatcatcgc aaccagatca ggactgattt gtgggtttac cgatactacg gtcgcaatca
    17641 cttgaatttc gttaaagaaa ccttctggga aaagtggacg cacaggacgg tcgatcaagc
    17701 gagcgattaa ggtttcacct tcgctaggac gtccttcacg tttgaagaaa ccgcccggaa
    17761 tacgaccggc tgcgtaagta cgctcttgat aatctacggt taatgggaag aaatcttgcc
    17821 cttcttttac gtcttttttc gctactacgg taacaaatac ggttgtgtcg tccatacttg
    17881 ccattactgc tgccgttgct tggcgtgcga tagcaccggt ttctaaggtc acggtgtgct
    17941 gaccgtattt aaactgtttt acaattggag tcattgaaat tttcctttta aagaatttac
    18001 tattattgtc gtt
//
LOCUS       DANLYI010000030        17647 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.030, whole genome shotgun sequence.
ACCESSION   DANLYI010000030 DANLYI010000000
VERSION     DANLYI010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 17647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17647
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.030"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            complement(<1..355)
                     /locus_tag="PXD81_000762"
     CDS             complement(<1..355)
                     /locus_tag="PXD81_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005752829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HDL5536812.1"
                     /translation="MSIAQNHNFKDKVVVITGAGGVLCAYFAKEIAKTGAKVALLDIN
                     LEAAQNFASEIADEGYIAKAYQTNVLELESIKQARDEILKDFGTCDILINGAGGNNPK
                     ATTDNEFHDINLPENT"
     gene            complement(367..1638)
                     /locus_tag="PXD81_000763"
     CDS             complement(367..1638)
                     /locus_tag="PXD81_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005668000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HDL5536813.1"
                     /translation="MLWLFLGSLFLFLFLGVPVALSMLLSASFMLWKMDMFDAQLITE
                     NFVMGTNNFPLMAIPFFMLTGEIMKHGGISERIINFAMSMVGHIKGGLGYVVIISGLI
                     FAGLSGSAVADTAALGAILIPMMVARKYDVARSTGLICAAGIISVVIPPSIPMIVYGI
                     TAGASITKLFMGGMVPGFLMVLGLWLVWKFLYRNEEQTVEPKKTGKERWAAFKSAFLP
                     LLLPIIIIVGLRGGIFTPTEAGVVAAIYAAIISIILKGLTLDKIQDVFLGTIKTTAMV
                     MFVAAAAMISAFAVTVAQIPMELVEFIKGITDSPTVLMFVIMIFLLLVGCVMDLIPAV
                     LIFVPVLLPLLRAYNIDIAYFGIMMVINLSIGLITPPVGTVLYVGSGIAKIGIARLTK
                     GIYPFLLVYAAILFLLILFPEIIIVPMNWLS"
     gene            complement(1641..2120)
                     /locus_tag="PXD81_000764"
     CDS             complement(1641..2120)
                     /locus_tag="PXD81_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HDL5536814.1"
                     /translation="MQISTALDRTLKLLAIIALSTMIILVFFNAVLRYFFDSGIAWSE
                     EFARICFVYMIFLGIILVAKERGHLTVDIVVSALPAKVQMYLLFFADILVLVALSFIT
                     YGAYQLMLLTYTQKMPATEISSAFLYFAAVISSISYFAIVFTNLWKHIRLAFIKEEK"
     gene            complement(2181..3164)
                     /locus_tag="PXD81_000765"
     CDS             complement(2181..3164)
                     /locus_tag="PXD81_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005693870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HDL5536815.1"
                     /translation="MKLKTFTKALLISSLMFTGSAFAKTIIKIGHYNSDVHPSNIALQ
                     EYFKKTIEEKTEGRYEIRIFPNNQLGGEDQIVNGLRNGTIEGGITGLLLQNVDPIFGV
                     WEWPYLFKDNAEAKKVLESPIAQEIGEKMEKYGIKLLAYGMNGFRVISSNKKVENFDG
                     FKGLRLRVPLNSLFVDWAKAMHINPQSMPLSEVFTALEQKVIDGQENPYMLIKDSGLY
                     EVQKYIIESNHIFSPGLLQLSLKTWNKMSKEDQTIFLEAAKLYQEKEWELAMKMEQDV
                     KDFFHKNGKEVIVPSEQFKADMLKASETLYQNFYQKYDWAKDVISKIHAAK"
     gene            complement(3193..4218)
                     /locus_tag="PXD81_000766"
     CDS             complement(3193..4218)
                     /locus_tag="PXD81_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HDL5536816.1"
                     /translation="MEKPLKSVCLVEPNKVGIQTVSYPVKGENDVLIKVEGMGICGSD
                     IGAFRGTNPLVTYPRILGHEIVGTVIESGKGMPNNINVGDRVIIEPYIYCGHCYPCSI
                     GRTNCCESLKVLGVHIDGAMQEVVRHPAHMIIKTPDMPIHELALAEPLTISLHAIHRT
                     KVKAGEHVAIIGAGAIGLMAALVAKAYGATPILIDILDKRLDYAKSIGIPHTINPTKE
                     NDLEAIKSITNGRMAEVVIEASGANVAVQNTLKYTSFAGRIALTGWPKTETPLPTNLI
                     TFKELNIYGARTSKGAFEEALELLASRRIEPKDIISKVITFDEIPHYIEELSANPDDY
                     LKIIAVF"
     gene            4382..5143
                     /locus_tag="PXD81_000767"
     CDS             4382..5143
                     /locus_tag="PXD81_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HDL5536817.1"
                     /translation="MQNSSRTYTKIGKILKEEIAQGKYRVGDKLPTERDISERFEVSR
                     TIVREAIVMLEVEKLIEVKKGSGVYVIRVPELFPEDFPEEKDNELRDVGPFELLQARQ
                     LLESSIAEFAAQQATKQDIQVLRNILESEKKLLLEKMDDYSADKEFHLALAHMTGNDV
                     LIQLQEQLWRYRFNSAMWAQLHSRILQHDHHHLWIEDHERILNAIQRKNPALARKAMW
                     QHLENVKTKLLELSDVEDPKFDGYLFNVNPVVVGI"
     gene            5173..6357
                     /gene="uxuA"
                     /locus_tag="PXD81_000768"
     CDS             5173..6357
                     /gene="uxuA"
                     /locus_tag="PXD81_000768"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012341261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HDL5536818.1"
                     /translation="MEQAWRWYGPNDPVSLDDVRQAGATGIVTALHHIPNGQVWSVEE
                     INKRKALLAEKGLVWSVVESVPVHEEIKTQTGNYQQWIDNYKQTLRNLASCGIDTVCY
                     NFMPVLDWTRTDLEYEMPDGSKALRFDQVAFAAFELYILKRPGAEATYSSAEQAEAKA
                     YFEQMSDADIAKLTKNIIAGLPGSEEGYTLEEFQAQLDRYQGLSAEKFRTHLAYFLNE
                     IVPVAQEVGIRMAIHPDDPPRPILGLPRIVSTIEDMQWFVETQPLAANGFTFCTGSYG
                     VLAENDLVKMAEQFADRIYFAHLRATQREENPQSFHEADHLAGDVDMFGVVKALLTEE
                     YRRKANGDHRLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLRGLAEFRGLELALKKVYF
                     ND"
     gene            6408..7277
                     /locus_tag="PXD81_000769"
     CDS             6408..7277
                     /locus_tag="PXD81_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDL5536819.1"
                     /translation="MFKKYFGEIILFLVTFIAAAGWFFSKHALVGLPPIGFMGGRFIL
                     ASLILLPFSYRELYRLDKVQVKYSVITGLTYGIYTIFWVLGLAFSTVFGEGAFLVSLA
                     MLIAPLLSWLIFKHTPPKIFWLALPIALSGLYFLSAEKGIFHFSLGSTIFLVSSVFGA
                     LYFVLNNQYAKQVSLFPLITIQLAIVGAVCSLYSYFMESWHFPILTEVWLWFAASVLI
                     ATNLRFLMQTLGQKYCHIGTSALIMLLEPVWTLLLSVMLLGEQLSATKWLGCSLILIA
                     LIIYRLPILRLKK"
     gene            7445..7759
                     /gene="rsfS"
                     /locus_tag="PXD81_000770"
     CDS             7445..7759
                     /gene="rsfS"
                     /locus_tag="PXD81_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015674146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HDL5536820.1"
                     /translation="MEQNLVEFLTKTLDDLKAQDILAIDVKGKSSITDTMILATGTSS
                     RHVASTAERLITEAKQAGFEVFGDEGKAVADWIVVDFGKAMVHLLQSDSREMYQLEKL
                     WA"
     gene            7836..8303
                     /gene="rlmH"
                     /locus_tag="PXD81_000771"
     CDS             7836..8303
                     /gene="rlmH"
                     /locus_tag="PXD81_000771"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010618862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HDL5536821.1"
                     /translation="MKIQLIAVGTKMPAWVTTGFEEYQRRFPKEMPFELVEIPAGKRG
                     KNADTKRILELEGKAMLAAAGKAKIVTLDIPGKAWTTEQLATQLEGWKNDGRDVALLI
                     GGPEGLSPECKAAAEQSWSLSPLTLPHPLVRVVVAESLYRAWSVTINHPYHRE"
     gene            8327..10276
                     /gene="mrdA"
                     /locus_tag="PXD81_000772"
     CDS             8327..10276
                     /gene="mrdA"
                     /locus_tag="PXD81_000772"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HDL5536822.1"
                     /translation="MFGKLAKFTASNQREKVRDGQAESNLYARRALVAFLGVLALSGV
                     LLTNLYHLQVVNYEDYQTRSNGNRIKLLPVPPARGLIYDRNGKVLAENLTYFGLFIVP
                     EKTENIEQTLTELKDIVGLTDEDIENYHKEKKRSSRYTPIMLKGNLNEAQMARFAVNQ
                     YRFPSLDVQAYYKRNYPYGELLTHIIGYVAKINENDKKKLQEAGKFGNYAGSHDMGKL
                     GIEKFYEDQLHGVTGFEEVEINNRGKVIRKLRDQAGVAGSSIRLSIDVELQQYVMSLI
                     AKHKGAVVVLDPRDSSILAMVSNPSYDNNLFVGGISGQDYKALLDDPTRPLYSRTTQG
                     TYPPASTIKPFMAVAGLEEGMITPSTTISDPGYWILPGTTQRYRDWKRSGHGATNVNK
                     AITESSDTFFYQLAYKMGIDKMSDWMKKFGFGVKTGIDIFEESAGVMPTREWKQKRYK
                     KPWVQGDTIPVGIGQGYWISTPLQLAKATAVLINNGRVNTPHLMLEVQSSNSVTPYQD
                     PLLYEDIKGVPDVYWNLAKQGMYNVNHAANGTARKVFAGAFYHSAGKTGTAQVFNLNG
                     KNYDKNAIKKELHDHAWFIGYAPYEQPKIVVALILENAGGGGSAAAPVARQIMDYALR
                     KGIGEQPDSQNSEQNKPLVTEEHHE"
     gene            10269..11396
                     /gene="rodA"
                     /locus_tag="PXD81_000773"
     CDS             10269..11396
                     /gene="rodA"
                     /locus_tag="PXD81_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HDL5536823.1"
                     /translation="MNSGFKKLFWKIFSLDLLLLSGLLAITGYGLLVLYSASGANERM
                     FTSRLIQVSLGLGLMLIMAMVPPRFYQKVSPYLYIFCLILLILVDLVGETSKGAQRWL
                     NLGFVRFQPSEIAKLAVPLMVATYLGKRPLPPSFKDTFIALAIIIVPTLLVAIQPDLG
                     TSILVCSAGIFVLFLAGLSWKLIGAGVVFLAGFIPIMWFFLMHDYQKTRVMTLIDPNK
                     DPLGAGYHIIQSKIAIGSGGINGKGWMEGTQSQLEFLPEPHTDFIFAVLSEEHGMIGV
                     LILLAIYLFIIARGLVIGTKSDSAFGRILSGGTALLFFVYVFVNIGMVSGILPVVGVP
                     LPLFSYGGTSYVTLMAAFGLMMSTYVHRKRVGSNNPYDKLK"
     gene            11488..12147
                     /locus_tag="PXD81_000774"
     CDS             11488..12147
                     /locus_tag="PXD81_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HDL5536824.1"
                     /translation="MRLGKIVTLLLAGFLTFSSLNSLAATKNTKAKSSILAKKQATSK
                     AIKKVGISKVKKKEVVAKATKKKTISTVTTKPIKSTKKLARKVVKKTVTAKKKLVKKQ
                     HKPLVKKHFQTGVASYYANYFNGRRTANGETFNNAKMTAAHRTLPFGTLIEVTNLRNG
                     RSVVVRVNDRGPYAHARVLDLSSAAAKKIGMHHSGTARVKIAIVDKSKLGSTNQFYVM
                     N"
     gene            12161..13339
                     /locus_tag="PXD81_000775"
     CDS             12161..13339
                     /locus_tag="PXD81_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005602317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HDL5536825.1"
                     /translation="MKKTLVKAMSVVGFAVSSIAYADLNTNFNLSAPQINAQTYILMD
                     YNSGEVLVSLNPDQRQYPASLTKMMTSYVVGEALKSGKVQNSDMVIVTENSWAQKFPG
                     SSLMFLDLNSKVSVGELMKGLIIVSGNDASVALAEHVSGSQAAFINEMNKYAQQFGLK
                     NTHFTTVHGLDDPEQYSSARDMAIIGSHIIRDQPEEYKIYAEKEFQYNIKKPQPNRNG
                     LLWDKTIQVDGMKTGHTDKAGYNLVASAINPNTRLISVVMGVPTYKGREVESKKLLQW
                     GFANFETIKSIPAAQSVAEQSVYYGEVNKVQLGSVQDSFVTIPKGRSADLKARYELEK
                     KNLEAPLAKGQVVGKMIYQLDGKDVASTNLQVLQDVPEAGIFGKAWDWIALTVKSLFD
                     "
     gene            13507..13806
                     /locus_tag="PXD81_000776"
     CDS             13507..13806
                     /locus_tag="PXD81_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbeD family protein"
                     /protein_id="HDL5536826.1"
                     /translation="MSQPKAINLQDLPQAKLKDLLEFPCHFTFKVVGANREDLVDDVV
                     VVTQKYAKGDYNPRQQKSSKGTYNSVSIDIVAENIEQVETLYTELAKINGVRMVL"
     gene            13868..14521
                     /gene="lipB"
                     /locus_tag="PXD81_000777"
     CDS             13868..14521
                     /gene="lipB"
                     /locus_tag="PXD81_000777"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HDL5536827.1"
                     /translation="MKLIIRQLGIQPYEEIWHQMQSFTDSRDGNTDDEIWLVQHPAVF
                     TQGSAGKPEHLLNPTNIPVVQSDRGGQITYHGEGQQIMYVLIDIKRLKSLGKEVSVRD
                     LVTALEQTVVQTLAEYGIESYPKADAPGVYVDGKKICSLGLRIRKGCSFHGLALNINM
                     DLTPFQNINPCGYAGLEMCQLSELVHRPDLRCEEVAPKLVAHFSTILGYNNQQIINN"
     gene            14536..15543
                     /gene="lipA"
                     /locus_tag="PXD81_000778"
     CDS             14536..15543
                     /gene="lipA"
                     /locus_tag="PXD81_000778"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HDL5536828.1"
                     /translation="MATSTAQTTATATVKKMSPFKMEKGVKYRDAAKTSVIQVRNIDP
                     DQELLKKPEWMKIKLPANSAKIDSIKNGMRRHGLHSVCEEASCPNLHECFNHGTATFM
                     IMGAICTRRCPFCDVAHGKPLPLDPDEPRKVAETVQDMKLKYVVITSVDRDDLPDRGA
                     AHFSATVREIKALNPHCKVEILVPDFRGRVEQAVAILKENPPDVFNHNLENVPRLYRE
                     VRPGADYKWSLELLKIFKQEFPHIPTKSGLMVGLGETNEEILEVMQDLRDHGVTMLTI
                     GQYLQPSRHHLKVERYVPPAEFDMFREEAEKMGFEHAACGPFVRSSYHADLQAKGELV
                     K"
     gene            15730..16833
                     /locus_tag="PXD81_000779"
     CDS             15730..16833
                     /locus_tag="PXD81_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL5536829.1"
                     /translation="MKKLLLNSVFAATLLAFGATANAEIKTITDVLGREVKVDVPAKR
                     VALTFYYPDYIAVTGVENFDKVVGISREFWEKFNPGSWALFAEKMPNLKNIADLGYVN
                     SGTFSTEKTIALKPDVLVLPEIQYQALASEISRIEQAGIPVVVDFNKQTIENHTKSVR
                     VFGQLTGTEERAEKIAKEYEEGIADIQSCIDNAKVSKPKIYAEFGNKGPKEHSFTFGK
                     NMWGAIAETVRGDNISAPFVENWGQINPEQVLVSKPDVIMISGTELGNDTNPEIMSMG
                     INIEEADAQKRLKGFIERTGWKELPAVKSGRVYGLYHTASRSISDLASAQFMAKALYP
                     ELFKDIDPEKTYLDFHNNYLPITPKGTFFMQLK"
     gene            complement(16924..>17647)
                     /locus_tag="PXD81_000780"
     CDS             complement(16924..>17647)
                     /locus_tag="PXD81_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL5536830.1"
                     /translation="VVADKINLHFEQGKIYTLLGPNGAGKSSLLKTLFGEIAHQGTID
                     FDGNKLTSSHLHSWRKEIGYMPQDTQVEASLSALEVILLGQLDSLNMYISDELLNEAV
                     DIMDKLGISHLAHQDIMRLSGGQRQMVMFAQVLLRKPHILLLDEPVSALDMYHQINLL
                     EYVNAYTRANNLITIMVLHDLSLAAQFSDSVMLLSEGKFKHKAELVICWNAILFRDSI
                     MSILRFYMMETARRLSARFANV"
BASE COUNT         5336 a         3302 c         3820 g         5189 t
ORIGIN      
        1 ttgtattttc cggtaagtta atatcgtgaa attcattatc tgtcgttgct tttggattat
       61 tcccacctgc accattaatt aaaatatcac aagtgccaaa atcttttaga atttcatctc
      121 ttgcctgttt tatgctttct aattcaagta cattagtttg gtacgctttc gcaatatagc
      181 cttcatcggc aatttcagaa gcaaaattct gtgctgcttc tagattgata tcgagtagtg
      241 caacttttgc acctgttttt gcaatttctt ttgcaaaata agcacatagc acaccacctg
      301 ctccggtaat aacaacaact ttatctttaa aattatgatt ttgggcaata gacataataa
      361 gaactcctaa gataaccaat tcattggtac gataataatt tcagggaaaa gtattaataa
      421 gaacagtatt gcagcataga ctagcaagaa tggatagata cctttggtta atctagcaat
      481 accaattttt gctataccag aaccaacata taataccgtt ccaacaggag gcgtaattaa
      541 accgatagat aggttaatta ccatcataat accaaagtaa gcaatatcga tgttatacgc
      601 tcttagtaat ggtaatagta ctggtacaaa aattaatact gctgggatta agtccattac
      661 acagccgact aataataaga aaatcataat gacgaacatt agtacagtcg ggctatcggt
      721 aatacctttg ataaactcaa ctaactccat aggtatttga gcaacagtaa ctgcaaatgc
      781 agatatcatt gccgccgctg caacaaacat aaccattgca gttgttttga tagtgcctaa
      841 gaaaacatct tggattttat ctaaggttaa cccttttaag ataatgctga ttattgctgc
      901 ataaattgct gctacaactc ccgcttctgt tggtgtaaag atgccgcctc ttaaacctac
      961 gataataata attggaagta gcaatggtaa gaatgctgat ttaaatgcag cccaacgttc
     1021 tttacctgtt tttttcggtt caacggtttg ttcttcgttt cggtataaga atttccaaac
     1081 taaccataaa cctaaaacca ttaaaaagcc tggaaccatt cctcccataa ataatttggt
     1141 aattgatgca ccggctgtaa taccatagac aatcattgga atgcttggtg ggataactac
     1201 agaaataata cctgctgcac agattaagcc tgttgaacgt gcaacatcat attttcttgc
     1261 aaccatcatc ggaataagaa ttgcaccgag agccgcggta tcagcaacag cggagccgga
     1321 taaacctgcg aaaattaagc ctgaaataat cactacatag cccaatccgc ctttaatatg
     1381 gccgaccatg ctcattgcaa aattgataat acgttcggaa ataccgccgt gtttcataat
     1441 ttcaccggtc agcataaaaa acgggatagc catcagcgga aaattatttg tacccattac
     1501 gaaattctcc gtgattaatt gggcatcaaa catatccatt ttccatagca taaaactggc
     1561 actaagtagc atagagagag caacgggcac tcctaaaaag agaaagagga atagacttcc
     1621 taaaaatagc cataacatat ttatttctcc tctttaatga aagctagccg aatatgtttc
     1681 cataaatttg tgaatacaat agcgaaatag gagatagatg aaataactgc tgcaaaatat
     1741 agaaacgcag atgaaatttc agttgctggc attttttgtg tgtatgtcaa taacattaac
     1801 tggtatgcac cgtaagtaat aaatgacagt gcaactaaga ctaaaatatc agcaaagaac
     1861 agtaagtaca tttgtacttt tgccggtagt gcagaaacaa caatatcaac ggtgaggtgt
     1921 cccctttcct ttgcaacgag gattattcct agaaagatca tatagacaaa gcagattctg
     1981 gcgaattcct cagaccaagc tattccggag tcaaaaaaat atcgtaaaac cgcattaaaa
     2041 aagactagga taatcattgt agatagagca attatcgcaa ggagtttgag agttctatct
     2101 aatgctgtag aaatttgcat aaatttctcc ttcttcattt agatgggcga cctcttttat
     2161 ccggtcgcct cgcttattgc ttatttggct gcgtggattt ttgaaataac atctttagcc
     2221 caatcatatt tctgatagaa attttgataa agcgtttcgg atgcttttaa catatcagct
     2281 ttaaattgtt cactcggaac aatgacttct ttgccatttt tgtggaagaa atcttttaca
     2341 tcttgttcca tcttcatcgc taattcccac tctttttctt ggtagagttt tgctgcctct
     2401 aagaagattg tctgatcttc tttagacatt ttattccaag ttttcaaact aagttgtagt
     2461 aagccaggtg agaaaatatg attagattca ataatatatt tttgcacctc gtataaacca
     2521 gaatctttaa ttaacatata tggattttct tgcccatcaa tgactttttg ttctagtgcg
     2581 gtaaatactt cacttaaagg catactttga gggttaatat gcattgcttt tgcccagtcc
     2641 acaaacaacg aatttagtgg aacccttaat cgcaaacctt taaatccatc aaaattctca
     2701 acttttttat tggaagagat aacacggaag ccattcatac catacgctag taatttaatg
     2761 ccgtacttct ccattttttc cccgatttct tgagcaatag gagattctag aactttctta
     2821 gcttcggcat tatctttaaa tagatatggc cactcccata cgccaaaaat tggatcaaca
     2881 ttttgtagta ataaacctgt aatgccacct tcaatcgtgc cattacgcaa accatttaca
     2941 atttgatctt ctcctcccaa ctggttattt gggaaaatac ggatttcgta gcgtccttct
     3001 gttttttctt caatggtttt tttgaagtat tcctgcaatg cgatatttga tggatgaaca
     3061 tctgaattat agtgaccaat cttaatgata gtcttcgcaa atgccgaacc ggtgaacatc
     3121 aggctagaaa ttaatagagc tttagtgaaa gttttgagtt tcatagttat tacctcgtag
     3181 gtgagttagt atttaaaata cagcgatgat ttttaagtaa tcatcaggat tagctgagag
     3241 ttcttcgata tagtgaggta tttcatcaaa tgtaattacc ttactaatga tatcttttgg
     3301 ttcaattctt cttgatgcca gaagctctaa tgcttcttca aatgccccct tgcttgttct
     3361 tgccccataa atattaagtt ctttgaaggt gattaaatta gttggtaatg gtgtttctgt
     3421 tttcggccat ccggttaaag caatacgtcc agcaaaagag gtgtatttaa gcgtattttg
     3481 tactgcgaca tttgctcctg acgcttcaat aaccacctct gccattcttc cattggtaat
     3541 tgatttaata gcttcaagat cgttttcttt tgttggatta attgtgtgtg gaataccaat
     3601 agattttgcg taatccaatc gcttatctaa aatgtcaatt aaaatcggcg tcgctccata
     3661 agctttggca accaatgcag ccattagccc aattgcccct gctccaatga tagcaacatg
     3721 ttcaccagct ttgacttttg ttctatgaat tgcgtgtaag gaaatagtta agggttctgc
     3781 taacgctaat tcgtgtattg gcatatcagg tgttttgatt atcatatggg ctggatgtct
     3841 aacaacctct tgcattgccc catcaatatg tacccccaat acttttagag attcgcaaca
     3901 atttgttcta ccaatggagc agggatagca atgtccacaa tagatgtaag gttcaatgat
     3961 gacacgatct cctacattaa tattatttgg cataccttta ccagactcaa ttactgtgcc
     4021 aacgatttca tgccctaaaa ttctaggata agtaacaagt ggattagtgc ctctaaatgc
     4081 tccaatatca gagccacata tccccatacc ttctactttg attagcacat cattttcacc
     4141 tttaacagga taagaaacgg tttgtatacc aactttatta ggttctacaa gacatactga
     4201 tttgagtggt ttttccatga ttttgtcctt ttttgataaa gttaatcgac attttgtagt
     4261 gtcaatggta ttttatgcac agatactaac accaaaaaac aagtttggca ttccaatttt
     4321 tgtgattttg tgaacctgat cacaaaaatg aaattttgga attccaattt cgtgaggatt
     4381 tatgcaaaat agttccagaa cttacactaa aataggaaaa attctcaaag aggaaattgc
     4441 tcaaggaaaa tacagagtag gagataagtt acctactgag agagatattt ctgaaagatt
     4501 tgaagtgagt agaactatcg taagggaggc gatagtgatg ctagaagtag aaaagcttat
     4561 tgaggtaaaa aaaggatcgg gcgtgtatgt cattagagtc ccggagcttt ttccagaaga
     4621 ttttccagaa gagaaagata atgagctaag ggatgttggg ccatttgaac tacttcaagc
     4681 acgacaatta ttagaaagca gtattgctga gtttgcagca cagcaggcta ccaaacaaga
     4741 tatacaagta cttcgtaaca tattagaaag tgagaagaaa cttttgttag agaagatgga
     4801 tgactacagt gcagataagg aatttcatct tgcattagct catatgacgg gtaacgatgt
     4861 tctgattcaa ttacaagaac agctttggcg ataccgcttt aatagtgcga tgtgggctca
     4921 gctacactcc agaattttac agcatgatca tcaccacttg tggatagagg atcatgagcg
     4981 aattttaaat gcaatacaga gaaaaaatcc tgcattagct cgtaaagcaa tgtggcaaca
     5041 tctagaaaat gttaaaacta aattgcttga attgtctgat gtagaggatc ctaaatttga
     5101 tggatatcta ttcaatgtaa accccgttgt agtaggtatc taaaacctaa actacacaat
     5161 gaggagttta ctatggaaca agcatggcgt tggtatggac caaacgatcc ggtatcttta
     5221 gatgatgtaa gacaagcagg ggcaacgggg attgtgacag cgttgcacca tattcccaac
     5281 gggcaggtgt ggtcggttga ggaaattaat aaacgtaagg cattattagc tgaaaaaggg
     5341 ctggtttggt cggtggtaga aagtgtgcct gttcacgaag aaatcaaaac tcaaacaggc
     5401 aactatcagc aatggattga taactataag caaaccttgc gtaatttggc aagctgtggg
     5461 attgatacgg tgtgctataa ctttatgccg gtgctagatt ggactcgtac cgatctggag
     5521 tatgaaatgc ctgatggctc aaaagcattg cgttttgacc aagtggcgtt tgcggcgttt
     5581 gagttatata tcttaaaacg cccgggagcg gaagccactt actcatcagc agaacaagcc
     5641 gaagccaaag cctattttga gcagatgagt gacgctgata ttgcaaaatt aaccaaaaat
     5701 atcattgccg gtttgccggg gtctgaggaa ggctatacgc tcgaagaatt ccaagcacag
     5761 cttgaccgct atcaagggct ctcggctgaa aaattccgta cccatttagc ctatttctta
     5821 aatgagattg tgccggttgc acaagaggtg ggcattcgta tggcgattca ccctgacgat
     5881 ccgcctcgcc cgattttagg cttgccacgc attgtttcaa ccatagaaga tatgcaatgg
     5941 tttgtggaaa ctcaaccgct tgcagccaat ggcttcacgt tctgtaccgg ttcatacggc
     6001 gtattagccg agaatgattt ggtcaaaatg gcagagcagt ttgcagatcg catttatttt
     6061 gctcacttac gagctaccca acgtgaagaa aatccacaaa gtttccacga agcggatcat
     6121 cttgccggtg atgtcgatat gtttggcgtg gtgaaagcat tacttaccga agagtatcgc
     6181 cgcaaggcaa atggcgatca ccgattaatt ccaatgcgtc cggatcacgg ccatcaaatg
     6241 ctcgatgatc tgaagaaaaa aaccaaccca ggctattctg cgattggtcg tttaagagga
     6301 ttagcggagt tccgtggatt ggagttggca cttaagaaag tctattttaa cgattagcct
     6361 tatttattat aatgccctct actgtagagg gcattttata gtttgatatg ttcaaaaaat
     6421 atttcggcga aataatcctt tttctcgtta cctttattgc tgcagcgggg tggtttttct
     6481 ctaaacacgc tttagttgga ttaccgccta ttggctttat gggagggcgt tttatattag
     6541 cctctttgat tcttctgcct ttttcttacc gagagctgta tcgtttagat aaggttcaag
     6601 ttaaatattc ggtgattacc ggtcttactt acggaattta tactattttt tgggtattgg
     6661 ggctggcttt tagtacagtg tttggtgaag gggctttttt ggtgagcctt gcgatgctga
     6721 ttgctccttt gctctcttgg ttgatattca aacatacgcc accgaagatt ttttggttag
     6781 ccttacccat tgcattaagt gggctttact ttctttctgc ggagaaaggc atctttcatt
     6841 tttccttagg cagtacgatt tttctggtgt catcagtgtt tggggcgtta tattttgtgt
     6901 taaataatca atacgccaaa caagtctcat tgtttccatt aattactatc caattggcga
     6961 tagtcggtgc ggtctgtagc ttgtattcct attttatgga aagttggcat ttcccgattc
     7021 tcaccgaagt gtggctctgg tttgccgcaa gtgtattgat tgcgacaaat ttacgttttt
     7081 taatgcaaac actggggcaa aaatattgcc atattggtac ttctgcactc attatgttac
     7141 ttgagccggt ttggacattg ttattgagcg tgatgttatt gggggaacaa cttagtgcga
     7201 caaaatggct aggttgcagc ttgattttga tagcattgat tatttatcga ctccctattc
     7261 tacgactcaa aaaataggta aaacaaaagc aaatatgcca tagtattgga agcagttagg
     7321 cattttagag taagtaacaa gcggtcattt ttgcaaaaaa tcttgcaaaa atgaccgctt
     7381 tctttctttt atccgttcaa tctatgttaa aattctacat ttttataaga tgagagagag
     7441 aaacgtggaa cagaatttag tcgaattttt aaccaaaacc ttagatgatt taaaggcaca
     7501 agatatttta gccattgatg tgaaagggaa atctagcatt actgatacga tgattttagc
     7561 caccggtaca tcgagccgtc acgttgcttc aacggcagag cgtttaatta cggaagcaaa
     7621 acaggctggg ttcgaggtat ttggagatga aggtaaagca gttgccgatt ggatcgtggt
     7681 cgattttggg aaggcaatgg ttcacttgtt gcaatccgat agccgtgaga tgtaccaatt
     7741 agagaaactt tgggcgtagc agttcaataa ggaacgacaa gcggtgattt ttgcaacatt
     7801 ttttgcaaat taaaccgctt gtaagtgaaa gagagatgaa aattcaatta attgcggttg
     7861 gcactaaaat gccggcgtgg gtaacgacag ggtttgaaga gtatcaacgc cgttttccga
     7921 aagagatgcc gtttgagttg gtggaaattc cggcaggaaa acggggcaag aatgctgata
     7981 ctaaacgcat tcttgagctt gaaggcaaag caatgttggc ggcagcgggc aaggcaaaaa
     8041 tcgtgacttt ggatattccg ggcaaagcgt ggacaaccga gcagttagcc actcagttag
     8101 agggctggaa aaacgatggg cgagatgtag cgttattgat tggcggcccg gaaggattat
     8161 caccggaatg caaagccgct gccgagcaga gttggtcgtt gtcgccttta accttacctc
     8221 acccactggt gcgggtggtg gtggcggaaa gtttgtatcg ggcgtggtcg gttaccatta
     8281 atcaccctta tcatcgagag tagtgagtca ttttagatag tgttatatgt ttggtaaatt
     8341 agcaaaattc acagcttcaa accagcgaga aaaggtgcga gacgggcagg cagaaagcaa
     8401 tctgtatgcc cgccgtgcct tagttgcgtt tctgggggtg ttggcgttaa gtggtgtttt
     8461 attaaccaac ttgtatcact tgcaagtggt gaattatgag gactaccaaa cccgttcaaa
     8521 cggtaaccgc attaaattgt tgcctgtgcc accggctcga ggcttaattt atgaccgtaa
     8581 cggtaaagta ttggcggaaa atttaaccta tttcgggctg tttattgtgc ctgaaaagac
     8641 ggaaaacatt gagcagactt taaccgagtt gaaggatatt gttgggctga cggacgaaga
     8701 tatcgagaat tatcataaag agaaaaaacg cagttctcgc tataccccaa ttatgctcaa
     8761 aggcaatttg aatgaggctc agatggctcg ttttgcggtg aaccaatacc gttttccaag
     8821 tttagatgtg caggcgtatt ataagcggaa ttatccttac ggcgagcttt taacccatat
     8881 tatcggttat gtggcgaaaa ttaacgaaaa cgataagaaa aagctgcaag aagccgggaa
     8941 attcggcaat tatgccggtt cgcacgatat ggggaaattg gggattgaaa aattctatga
     9001 agatcagctc cacggtgtca ccggttttga agaggtagaa attaataacc gagggaaagt
     9061 aatccgtaaa ttgcgtgacc aagcaggggt tgccggtagc agtattcgct tgagtattga
     9121 tgtggaatta cagcaatatg tgatgtcgtt aattgctaag cataaagggg cggtggtggt
     9181 gcttgaccct cgtgatagca gtattttagc gatggtctca aacccaagtt atgacaataa
     9241 tttatttgtg ggcggtattt cagggcagga ttataaagca ttattggacg atccaacccg
     9301 accactttat agccgtacta cacaaggcac ttatccaccg gcttcaacta ttaagccatt
     9361 tatggcggtg gcaggtttgg aagagggaat gattacccct tctaccacca tttcagaccc
     9421 cggctattgg attttgcccg gcactacaca acgttatcga gattggaaac gtagcggaca
     9481 cggtgcaact aatgtgaata aagcaattac cgaatcgtct gatacctttt tctatcaatt
     9541 agcctataaa atggggattg ataaaatgtc ggattggatg aaaaaattcg gtttcggagt
     9601 gaaaaccggt atcgatattt ttgaggaatc cgcaggggta atgccaaccc gtgaatggaa
     9661 acaaaaacgc tataaaaaac cttgggtgca gggcgatact attccggttg ggattgggca
     9721 gggctactgg atttcgaccc cgttacagtt ggcgaaagcc acggcggtgt taatcaacaa
     9781 cgggcgggtg aatacaccgc atttaatgct tgaagtgcaa agcagtaatt cggttacgcc
     9841 gtatcaagac ccattgttgt atgaagatat taaaggcgtg ccggatgtct attggaattt
     9901 agcaaaacag ggaatgtata acgtaaacca cgccgcaaac ggtacggcac gaaaagtgtt
     9961 tgccggtgca ttttatcatt cagcagggaa aaccggtacg gctcaggtgt ttaacttaaa
    10021 cggtaaaaat tacgataaaa atgcgattaa aaaagagttg cacgaccacg cttggtttat
    10081 cggctatgct ccttatgaac aacctaaaat agtggtggca ttaattttag aaaatgcagg
    10141 cggtggtggt agtgccgcgg ctcctgttgc tcgtcagatt atggattatg ctttgagaaa
    10201 aggcattggt gaacagccgg attcgcaaaa ttctgagcaa aataaaccgc ttgtcacaga
    10261 ggaacatcat gaatagtggc tttaaaaaac ttttctggaa aattttttca ctcgatctcc
    10321 ttttattatc aggattatta gctattaccg gttatggttt attagtacta tatagcgcaa
    10381 gcggtgcaaa tgagcggatg tttaccagta gactgattca agtttcgcta gggcttgggc
    10441 taatgttgat aatggcaatg gtacctcctc gcttttatca aaaagtttcg ccttatctgt
    10501 atattttctg tctgatatta ttgattttgg tggatttggt cggcgaaacc agtaaaggag
    10561 cgcaacgttg gttgaattta ggatttgttc gctttcagcc ttctgagatt gccaagctcg
    10621 ctgtgccact gatggtagcc acttatttag gtaagcggcc gcttcctccg agttttaaag
    10681 atacttttat cgccttagcc attattattg tgccaacctt attggtggca attcagcccg
    10741 atttgggaac atctatttta gtgtgttctg ccggtatttt tgttttattt ttggcagggc
    10801 taagttggaa attaattggc gcaggtgtgg tgtttttagc cggttttatc ccgataatgt
    10861 ggtttttctt aatgcacgat tatcaaaaaa cccgggtgat gaccttaatt gatcctaata
    10921 aagacccgtt gggtgccggc tatcatatta ttcaatccaa aattgcgata ggctctggtg
    10981 gcattaatgg aaaaggctgg atggaaggaa cgcaatccca gcttgaattt ttacctgaac
    11041 cgcataccga ttttattttt gcagtgttaa gcgaagagca tggtatgata ggtgtgctga
    11101 ttttattagc gatttatttg tttattattg cccgaggttt agtgattggc acaaaatcgg
    11161 attctgcttt cgggcgtata ttatcaggtg gtacagccct tctatttttt gtttacgttt
    11221 ttgtaaatat tggtatggtg agtggtattt tacccgtagt cggtgtacca ttaccgcttt
    11281 tcagttacgg cggaacctcc tatgtgacgc tgatggcggc atttggtttg atgatgtcaa
    11341 cctatgttca ccgtaagcgt gtaggtagta ataatcctta tgataaactg aaataaatta
    11401 aacttttgct tttttgcggt atcaaaaagc agtgaaacga tcatcgttac aaacaataac
    11461 gacatccaca tagaggaaaa aacttatatg agattaggta aaattgtaac cctgttgtta
    11521 gctggatttc tgacatttag ttcattaaat tctttggctg cgacaaagaa tacaaaagct
    11581 aaatcgagta ttttagccaa gaagcaagca acttctaaag caataaaaaa agtaggtatt
    11641 tctaaagtta agaaaaaaga agtagtggct aaagctacaa aaaagaaaac aatttctact
    11701 gttacaacta agccgattaa atctacaaaa aagctggctc gcaaggttgt caagaaaacg
    11761 gtaaccgcta agaaaaaatt ggtaaaaaag cagcataagc cactggtcaa aaaacatttc
    11821 cagactggtg ttgccagcta ttatgcaaat tactttaatg gtcgccgaac cgctaatggg
    11881 gagactttca ataacgcaaa aatgacggct gcacatcgaa ctttaccatt tggtacttta
    11941 attgaagtga cgaatttacg aaatggtcgt tctgtggtgg tgcgtgtaaa tgatcgcgga
    12001 ccttatgctc acgcacgggt cttggattta tcatcagccg cagcgaaaaa aatcggtatg
    12061 caccattcgg ggacggcaag agtgaaaatt gcgattgttg ataagtcaaa attgggatcg
    12121 actaatcagt tttatgtaat gaattaattt aagagggaat atgaaaaaaa cattagtgaa
    12181 agcgatgagc gtggtaggtt ttgcggtttc aagcattgcc tatgcggatt taaacactaa
    12241 cttcaattta tcggcacccc agatcaacgc acaaacctat attttgatgg attacaactc
    12301 cggtgaagtt ttagtgagcc taaatccgga tcaacgtcaa tatcctgcat cattgaccaa
    12361 aatgatgact agctacgtag ttggtgaagc cctaaaatcg ggaaaagtgc aaaacagtga
    12421 tatggtgata gtcaccgaaa attcgtgggc gcaaaaattc cccggctctt cattaatgtt
    12481 tttagattta aacagcaaag tttcagtagg tgagttgatg aaagggctga ttatcgtctc
    12541 gggtaatgat gcctctgtgg cgcttgctga acatgtttca ggctcacaag cggcgttcat
    12601 taatgaaatg aacaaatatg cccaacagtt tggcttaaag aatactcact ttaccactgt
    12661 tcacggttta gatgaccctg agcaatattc ttctgctcgt gatatggcga ttattggttc
    12721 gcatattatt cgtgatcagc cggaagagta taaaatttat gcggaaaaag agttccaata
    12781 taacatcaaa aaaccacaac caaaccgcaa cggattattg tgggataaaa ccattcaagt
    12841 tgatggtatg aaaacagggc atactgataa agccggctat aacttagtcg catcagcaat
    12901 taacccgaat acacgtttaa tttctgtagt gatgggcgtg ccaacttata aagggcgtga
    12961 agtggaaagt aagaaattac tgcaatgggg ctttgcgaac tttgaaacca ttaaatctat
    13021 tccggcagct caatcggtgg cagagcaaag tgtctattac ggtgaagtca ataaagtaca
    13081 attaggctcg gtgcaagata gctttgtgac tatcccgaaa ggtcgctcgg cagatttaaa
    13141 agcccgttat gagttagaga agaaaaacct cgaagctcct ttagcaaaag ggcaggtagt
    13201 gggtaaaatg atctaccaac ttgacggcaa agatgttgca agcactaact tacaagtctt
    13261 acaagatgta ccggaagccg gtattttcgg caaagcgtgg gattggattg cccttactgt
    13321 gaagagcttg ttcgattaat cttgttattt gcctttttat ccccatctag ggagcaatgc
    13381 aagcggtgtg attttgcaaa aaatttgcaa aatttcaccg cttaccgttt tagatatgaa
    13441 tgtataaaaa gtgtagtggc gaaaaatttt tcgcccctcg aagaaagaat agaagaagga
    13501 tcaaaaatgt ctcaacccaa agcgataaac ttacaagatt tacctcaagc caaattaaaa
    13561 gatctgctag agtttccgtg ccatttcacg ttcaaagtgg tgggagcaaa ccgagaagat
    13621 ttggttgatg atgtggtagt ggtcacgcaa aaatatgcca aaggcgatta caatccacgt
    13681 cagcaaaaaa gctcaaaagg tacctataat tccgtttcga ttgatattgt ggcggaaaat
    13741 attgagcaag tagaaactct ctacacggaa cttgccaaaa ttaatggcgt aagaatggta
    13801 ttataatgcg aggggcgaat gacattcgcc ctcaattttt caataaagcg atgttggaaa
    13861 atagaaaatg aaattaataa ttcgccaact aggcattcag ccttatgaag aaatttggca
    13921 ccaaatgcaa tctttcacag atagccgtga tgggaatacc gatgatgaga tctggctggt
    13981 tcagcacccg gctgtgttta cgcaaggatc ggcaggcaaa ccggagcatt tgttaaatcc
    14041 aaccaatatt ccggtggtgc aaagcgaccg tggcggtcaa attacttacc acggcgaggg
    14101 gcagcaaatt atgtatgtgc tgattgatat caaacggctg aaatccttgg gtaaagaagt
    14161 gtcggttcga gatttagtga ctgccctgga gcaaaccgtg gtacaaacgt tggcagaata
    14221 tggcatagaa agttatccaa aagccgatgc acccggggtg tatgtggacg gcaagaaaat
    14281 ctgctcactt ggtttacgaa ttcgaaaagg ttgctctttc cacggtttgg cattaaatat
    14341 caatatggac ttaacgcctt ttcaaaacat caatccgtgc ggttatgcag ggctggaaat
    14401 gtgccaatta agtgaactgg ttcatcgccc tgatttgcgt tgcgaagagg ttgctccaaa
    14461 attagtggct catttcagca caattttagg ctataataac caacaaatta ttaacaatta
    14521 ggataaaaca cagtaatggc tacatcgaca gctcaaacga cggcaactgc tacagtgaaa
    14581 aaaatgagcc ctttcaaaat ggaaaaaggg gtgaaatatc gtgatgccgc caaaacctct
    14641 gtgattcagg tgcgtaatat cgacccggat caagaactct taaaaaaacc ggagtggatg
    14701 aaaatcaaac tgccggcaaa ttcggcaaaa attgatagca ttaaaaacgg tatgcgtcgc
    14761 cacggcttgc actcggtgtg cgaggaagcc tcttgcccga atttacacga atgttttaac
    14821 cacggtactg caacctttat gattatgggg gcaatctgta cccgtcgttg cccgttctgt
    14881 gatgtggcac acggcaaacc gcttccgctt gacccggacg aacctcgcaa agtggcagaa
    14941 acggtgcaag atatgaaact caaatatgtg gtgattacct cagtggatcg tgatgatttg
    15001 cccgaccgtg gtgcggctca tttctctgcg accgtgcgtg aaatcaaagc cttaaatccg
    15061 cattgtaagg ttgaaatttt agtgcctgat ttccgtggac gtgtggagca agcggtggca
    15121 attctcaaag aaaatccacc tgatgtcttt aaccacaacc tagaaaatgt gccacgtctc
    15181 tatcgtgaag ttcgtccggg agcggattac aaatggtcgt tggaattatt gaaaatcttc
    15241 aagcaagagt tcccgcatat tccgaccaaa tccggcttaa tggtaggctt aggtgaaacc
    15301 aacgaagaaa tcttggaagt aatgcaagat ttacgagacc acggcgtgac gatgctcacc
    15361 atcgggcaat acttacaacc aagccgccac cacttaaaag tggaacgtta tgtgccaccg
    15421 gcagagtttg atatgttccg tgaggaagct gaaaaaatgg gctttgaaca cgcagcctgc
    15481 ggaccgttcg tgcgttcttc ttaccacgcg gatttacaag cgaaaggcga attggtgaaa
    15541 taaaatcgat aagggcgaat attatttgcc ctttgttttt gtggtttgca aagttttagc
    15601 aaaattaaac cgcttgtaat ctcttctaat ttactcttct atagataatt tcgttgttgc
    15661 gaattatttt cattgactat ttgttataga aagttacaat tcaactttcc atttcttttt
    15721 ggagttccaa tgaaaaaatt actattaaac agcgtatttg ctgccacatt gcttgctttt
    15781 ggtgcaactg ccaatgccga aattaaaacc atcactgatg tattagggcg tgaggtcaaa
    15841 gttgatgtgc ctgctaaacg tgttgcactg actttctatt accctgacta tattgcggta
    15901 accggtgttg aaaatttcga taaggtagta gggatttcac gagagttttg ggaaaagttc
    15961 aacccaggca gttgggcatt atttgcagaa aaaatgccaa atctaaaaaa cattgccgat
    16021 cttggctatg tgaatagtgg tactttttca acagaaaaaa ccatcgcatt aaaaccagat
    16081 gtgttagtgc tacctgaaat tcaatatcaa gcgttagcca gtgaaatttc acgcattgag
    16141 caagctggca ttcctgttgt agtagatttc aacaagcaaa ctattgaaaa ccatacaaaa
    16201 agcgtgagag ttttcggtca gcttacaggc actgaagaga gagcggagaa aattgccaaa
    16261 gagtatgaag agggcattgc tgatattcag agctgtattg ataatgcaaa agtaagtaag
    16321 ccgaaaatct atgctgaatt tggtaataaa ggcccgaaag agcatagttt tacctttggt
    16381 aaaaatatgt ggggagcgat tgccgaaacg gtgcgaggcg ataacattag tgccccgttt
    16441 gtagaaaact gggggcagat taaccctgag caggtgttag tttctaaacc tgatgtgatt
    16501 atgatttcag gtacggaact aggcaacgac actaaccctg agattatgtc gatggggatt
    16561 aatattgaag aagccgatgc tcaaaaacgc ttaaaaggct ttattgagcg tacaggttgg
    16621 aaagagttac ctgcggtaaa atcagggcgt gtttatggtt tatatcatac agcttctcgc
    16681 tcaatttcag atttagcgag tgctcaattt atggcaaaag cactttatcc tgaattgttt
    16741 aaagatatcg atcctgaaaa aacgtatttg gatttccaca acaactactt accaattacg
    16801 ccaaaaggca cattctttat gcaactgaaa taaaaataac aagcggtcat tttgcagatt
    16861 tattttgcaa aatgaccgct tgtattcgct tttactattt gcggttcaaa cagcaatcaa
    16921 tatttaaaca tttgcgaagc gggcggataa tcggcgagcc gtttccatca tataaaatct
    16981 caatattgac attatagagt ctctgaataa tatcgcattc caacatatca caagctctgc
    17041 cttgtgcttg aatttcccct cgcttaatag catcacacta tccgaaaatt gagccgcaag
    17101 gcttaaatcg tgcagcacca taattgtaat taggttattc gctcgagtgt aggcattcac
    17161 atattcaagt aagttgattt gatgatacat atccaacgca ctgacaggct catctagcag
    17221 cagaatgtgt ggtttacgta gcaatacttg tgcgaacatt accatttgcc gttgcccgcc
    17281 acttagccgc ataatatctt gatgagctag atgtgaaata ccgagtttat ccataatatc
    17341 aacggcttca ttgagtaatt catcgctaat atacatattc aggctatcaa gttgccctaa
    17401 taaaataacc tctaaggcac tgagtgaggc ctctacctgt gtgtcttgtg gcatatagcc
    17461 aatttcttta cgccaactat gcaagtggct tgaggttaat ttattaccgt caaaatcaat
    17521 tgtgccttga tgagcaattt ccccaaatag agttttaagg agagaagatt tacccgcacc
    17581 atttgggcca agtaaagtat agatcttgcc ttgttcaaaa tgtagattga ttttatcagc
    17641 caccact
//
LOCUS       DANLYI010000031        17192 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.031, whole genome shotgun sequence.
ACCESSION   DANLYI010000031 DANLYI010000000
VERSION     DANLYI010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 17192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17192
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.031"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            complement(199..1074)
                     /gene="ubiA"
                     /locus_tag="PXD81_000781"
     CDS             complement(199..1074)
                     /gene="ubiA"
                     /locus_tag="PXD81_000781"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HDL5536831.1"
                     /translation="MNNETLFSKQKWIAYAQLMRFDKPIGTLLLLHPTLWALFIAAEG
                     MPPVAILAIFTLGVIVMRAAGCVINDYADREIDGHVKRTSQRPLATGRVTTTEAKVIF
                     AILLLFAFLLDLMLNRYAFLLSFVAVFLAVIYPFMKRFTHLPQVVLGMAFGWSIPMAF
                     GAVTETLPLECWLLFFANLAWTVAYDTQYAMVDRDDDLRIGVKSTAILFAQYDNKIIA
                     LLQFITLILLGLLGVVKDYHIGYFVVLALSATLFIYQCWLTKKRERSECFKAFLNNHY
                     FGLGVFVAILVGIYF"
     gene            complement(1067..1828)
                     /locus_tag="PXD81_000782"
     CDS             complement(1067..1828)
                     /locus_tag="PXD81_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HDL5536832.1"
                     /translation="MKQANRHKKIIELVNQLGYVSTEQLVAELNVSPQTIRRDLNEMA
                     ENNLIRRHHGGAAAPSNTENSDYTHRKQFFSHEKNAIAQQVAKLIPNGASLFLDIGTT
                     SEAVALALLSHKNLKVVTNNLNAAHILMQNEDCQITVAGGGLRSDGGLIGEETVRFIN
                     QFRLDFGILGISAVDMDGSMLDYDYHEVQVKRALMECSRQVVLVTDHSKFTRTAIARL
                     GNVKEVNYLFTDQDLPLELQTHLSQSDVIVKICNE"
     gene            complement(1895..3262)
                     /gene="purB"
                     /locus_tag="PXD81_000783"
     CDS             complement(1895..3262)
                     /gene="purB"
                     /locus_tag="PXD81_000783"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HDL5536833.1"
                     /translation="MELTALTALSPIDGRYQDKVASLRPIFSEFGLLKFRVTVEVRWL
                     QKLASHTQINEVPVFSEKANDYLNQIVADFSLEDANRIKTIERTTNHDVKAVEYFLKE
                     KCEALPELQAVNEFIHFACTSEDINNTSHALMLKTAREEVLLPEWKKVIDAVAELAKC
                     YQHIPLLSRTHGQPASPTTMGKEMANVAYRLQRQYKQLENLEILAKINGAVGNYNAHL
                     SAYPEIDWHTFSQEFVESLGVTWNPYTTQIEPHDYIAEFFDCVARFNTILIDFDRDMW
                     GYIALNHFKQRTIAGEIGSSTMPHKVNPIDFENSEGNLGLANAVMAHLGQKLPISRWQ
                     RDLTDSTVLRNLGVGLGYALIAYASTLKGISKLEVNEQHLRDELNQNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDETAMREFIDKLEIPENEKNRLKEMTPASYIGYAVE
                     LVDKL"
     gene            complement(3296..3925)
                     /gene="hflD"
                     /locus_tag="PXD81_000784"
     CDS             complement(3296..3925)
                     /gene="hflD"
                     /locus_tag="PXD81_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005601117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HDL5536834.1"
                     /translation="MADYHDITLAYAGVCQAATLVQQFAHKGMADREAFKCALESLLI
                     TQPESTLSVFGDDLSHLKMGLETALAQTGGGNGKLDTEVGRYWISLLALSQKLNKNPE
                     AKQQLAQRLQQVERQLALYEDNVMADQMIANLAAIYSDIISPLGTKIHVMGMQDYLVR
                     PDIQNKIRASLLAGIRAGILWQQVGGSRWQFLFSRKKIFNQAQKFYRQI"
     gene            complement(4057..5721)
                     /gene="cydC"
                     /locus_tag="PXD81_000785"
     CDS             complement(4057..5721)
                     /gene="cydC"
                     /locus_tag="PXD81_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HDL5536835.1"
                     /translation="MKSLFPFFALYQTHFGRLLLGVVLAILGLAASIGLLSLSGWFLA
                     ASFLAGNALIFNFFYPSSGVRGLAVGRTISRYFERLVTHDATFRVLANLRASVFKKLI
                     PLSPSGLNRFRNSELLNRLVADVDTLDTLYLNLVSPFVSAVMIIAFMAIGLSFVSVPL
                     MLIICGTLLVLLAVIPTVFYRLGLKLGRNAIQSRANYRSQFIEWVQLNAEFLLFGNLN
                     QMSEKLQQTEQQWLNAQSKESRLSGLSNSLIMLSNGILTCVVIYLVSTSINVPTAKYP
                     EALIALVIFCVMASAEILSPIGIAFLHLGQVITAAERISEITEQQPNVTFGSKAEWQN
                     LSQNQPLVRCENVSFAYYGEQNVLNNLSFKVLKGQKVAILGKTGSGKSTIFQLLNRNY
                     DPTSGQIWLNNCKIDEYSEPMLRSKLVTLSQRVHIFSQTLKDNLLMGNAQATDEQMQE
                     AVRKVGLGHLLETDGLNLWLGEGGRPLSGGEQRRLGLARLLLSSAELVLLDEPTEGLD
                     RETEQQILNLILTHCCDRTLIMITHRQSGLDKFDVVYRIDNGRLVG"
     gene            complement(5721..7487)
                     /gene="cydD"
                     /locus_tag="PXD81_000786"
     CDS             complement(5721..7487)
                     /gene="cydD"
                     /locus_tag="PXD81_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HDL5536836.1"
                     /translation="MEKQRQRELQKWLKSQQKVIKKYMHLNILLGSFSSLCMIGQMWL
                     IATMLDKMLIGKQSPNSFLVEIMLLFCCFAARAFFIFLRERVGFKAGQTLRLHLRRQI
                     LAKMEAVGPMSIQQKPAGSWATLMLEQVENLHNFYARYLPQQFLSLIVPLLILCFVFP
                     INWAAGAILFATMPLLPLFMILAGMKAVEANQRNIGILSRISGQFLDKLKGLETIRLF
                     GQAEKQTEQIYQSTEDFRISTMDVLKMAFLSSAVLEFFTAVSIAVTAVYFGFIFLGEL
                     DFGYYGTGVTLFIGFFCLMVAPEFYQPMRELGVFYHDKAAAISAADNIETFLKEKVKT
                     QGGNLQENVENQPLVIQAKDCVIFSPQGKALTQALNFELNAHQHIALVGQSGAGKSSL
                     MNMLLGFLPYEGSVTINGTELRDLNLSQWRAKLAWVGQNPQLMHGSLKENILIGNPNA
                     TDSELAKALQLSKADEFVARLGLEHQVQDSNIGISGGQAQRIAIARALLRPYELLLLD
                     EPTASLDMDSEQQVLAALHNLSREQTTLMITHRVEDLTQCDEIWVMKQGQIIQKGRFT
                     ELEHTGFFAELIHNELVHQGEI"
     gene            complement(7602..11699)
                     /locus_tag="PXD81_000787"
     CDS             complement(7602..11699)
                     /locus_tag="PXD81_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB"
                     /protein_id="HDL5536837.1"
                     /translation="MTDENKQTATNQTDSAQEPQATQPKKSKWRFLKHGFCLTFILLL
                     LAILFLCTGYGQRKVLSWVDKFVEPLQLGQVQGSLQDGLQLSDARFVMDGVNVTVGQA
                     DLHIGFSCLLHYEMCLNNLSLKDTEVLIDTTKLPPSQPKEETEPFTELNLPLGISAQN
                     INLENIKVKVDDMDIHLNHFHTALSGKGRAITVHPTQLEGLNLLLALKLDETDTEQAV
                     DFAQKNAKTEALSATNPQEIADKVIQEAVEKTSEKVVEGFHQLQRKTVAQAEQVDNKV
                     DWAAIKAQLAQPLLDKNIRFSLPLDLNIEQIDIANVAIAQKAKDEKGNLIEPISLLNV
                     ASLQLQAQAKDQHINLKSLDFKSDQGDLTASGSLSLQDNYPLSWTLSGHEAKTAKVKL
                     PFNQIEASLSGELYDKTSLKVKTAGAISASLEGNVALAQAKTPFDLSLKSESMRYPFA
                     PQQNGDVLSLENIAMRLNGNLLDYALEAKVSAKGMGVPASSASLTGKGELTHFNIQDL
                     SLNTLEGTAKIDGLVDWSEGVAWRSNLKLQHINTKSLLADWPAVLSGSLSSEGYAGRG
                     NSASGWKADVSNIDIKGSLLQKNLHLSGNLKADHQQLLDVPGLSLVYGENKIDLKGVL
                     GEKSDFYADIKAPNLQGLVPNLKASVNGNAKLSGKVSEPNIDLDLVASNVGYEQFKLQ
                     NLTAKGKITTEKTIQGDLTLGLRQFAYNEIKVGNATLLLKGNEENHSLKLSAKGNPVG
                     ADLQISGKFDRLKEIWEGQLSQVSIQSTEFGQFQADKAVNVKYDNKAINANISAHCWH
                     NRKINLCFPTAFNAGVEGKVPFEIRNFDLAVLQQFLEKNSQLSGIVNAKGDAAWFKNK
                     QPQVNLDLTSNAIKFIQKMEGGKSFPLTVSPLKIDLKMSDNNLTLKSDLRVENNGRLS
                     TDLVMKDLSKARALSGNIHLDQLSLKLIRPLLERGDSVDGNVNARLTVSGTATSPLLH
                     GNLNVTELKARSVTMPFDITGGNLAMNFHGATSTLSGKLQTKQSELRLEGDADWRDLN
                     AWKTRVHAQANRFQVDIPNIAKVAVSPDIQVTATPTLLTLSGNVDIPWARIEVEQLPE
                     SAVSVSGDEVIMDGSVKNKVPFSQRNIPAQTSGGMAINADIKINIGNDVKLKAYGLNS
                     NVNGLLSVRQGKQGLGLYGQVHLREGRFAAYGQDLLIRKGDISFAGSPSQPTLDIEAI
                     RNPEAMEDPTITAGVRVTGLADNPTVKVFSDPAMSQNEALSYILTGRSLESSGDTSSS
                     NAVAAALLSMSLSKSSKLVGDVGSTFGLKDLSVTTAGIGDNTKVEVSASLAPKFRVKY
                     GVGIFAPLTELTLRYNLTPRLYLQWVSSINQAVDLMYRFEFDELF"
     gene            complement(11780..13615)
                     /locus_tag="PXD81_000788"
     CDS             complement(11780..13615)
                     /locus_tag="PXD81_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012262982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor"
                     /protein_id="HDL5536838.1"
                     /translation="MNTKLHFLTFCCLVAFASMAQANQEQRLSDVDRERVENLQEKLA
                     EDSDDEPEIEPLVTLEVKGIQGKDAKKNVELYLDQIANEYADGSERYQYLVQTTVDKA
                     LRALGYYNSRYQFVQLPQEGKKPLLVLNVELDKETVKIDETDIVLQGDAKTDEDFAHL
                     VSKAPKQGSRLNHKTYDNFKGDIESTAFSKGYFDGHWLYHRLEVYPTDHKADWRLGYD
                     SGKRYHYGQIRFVDSQIKEEYLQNILKIHSGEPYLANDLSILTQDYLSSNWFSSVMVE
                     PHLNEQDKLVDLNVLFQPKKRNEVEIGIGYETEVGPRLQLNWKKPWLNSRGHSIESRT
                     YVSKPEQTFEFGYNIPLREDPLHYYYQLSGTLENEDQNDTKSTAATLGLQRFWTHETG
                     WSFSVGLKARYDSFTQANDRFKTLLVYPTASLNRTRTDGKPYPLWGDTQKLTVNWGSR
                     AVGSDVNFYSYKASTSWARTYFDNHRFYLRAELGYLKANQFERIPPALRYFAGGDMSV
                     RGFGYKDISPRDPSNGKRVGGSHLATGTVEYQYQVYPNWWGAIFYDAGLASNKFKTQD
                     LYYGTGLGVRWASPIGAIKFDVATPVKSPDNSKGIQIYIGLGSDL"
     gene            complement(13627..14472)
                     /locus_tag="PXD81_000789"
     CDS             complement(13627..14472)
                     /locus_tag="PXD81_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HDL5536839.1"
                     /translation="MFKQINKRLNFNIFLQMFVLFTPLVQAAQMAIVIDDVGYRIKED
                     REILALPKAVSVAIIPVAPYATERAKDAYNQKRDILIHLPMEPKSKQPIEEGGIHIGD
                     NEEKIRKLIHTSRGQVPYAIGLNNHMGSGATSDSATMQHLLKVLKENTLFFLDSKTIG
                     SSVAAKTARQFGINTLERDIFLDDSDLLADVQKQFAHAINHARKNGVAVVIGHPRKNT
                     ISVLKQNLAQLPQDIELVSVGNLWRNEKTVPDKPFIMLFEQEPALSSVPPYDSIPLLR
                     GIPKE"
     gene            complement(14473..15636)
                     /locus_tag="PXD81_000790"
     CDS             complement(14473..15636)
                     /locus_tag="PXD81_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HDL5536840.1"
                     /translation="MLVKRINSYCWTAIFSGVISFSTPFSFANNLNQIQQKIQQQTSK
                     INEQKQKRNALQSTLKNQEVEMGKVLDKLQKTEMSLTEVRQTIKATETEIARLEKLEK
                     QQKEKLKEQLDSAYRSGVNPSVLERLMSEEAKNAERMTAYYQHMNNIRIDAIVEIRKT
                     QADLKARRDELKGQQKDHQTQLNEQKKQEKDLKKVQSQRESTLRLIDKTLEQDQSRLE
                     NLRSNEQALKQQLEQAQREAQQAEKREQQREKQAKSGQSKTTENVERTPIKAGRYTMP
                     VSGNIITPFGNNWHGVVIGASAGTSVRAMASGRVIMAQWLAGYGNMVAIDHGNGDISL
                     YGYNQSISVSKGSRVQGGQVIAKVGNSGGQSRSALYFGVTRKGTPINPLNLVK"
     gene            15804..16487
                     /locus_tag="PXD81_000791"
     CDS             15804..16487
                     /locus_tag="PXD81_000791"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011201466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HDL5536841.1"
                     /translation="MQLVFIRHGFSEWNAKNLFTGWRDVNLTERGIEEAKSAGQKLKA
                     AGFEFDIAFTSVLTRAIKTCNIVLEESDQLWIPQVKNWRLNERHYGALQGLDKKETAE
                     KYGDEQVHIWRRSYDISPPDLDPQDPNSAHNDRRYANLPKDLIPNAENLKITLERVLP
                     FWEDHIAPALISGKRVLVTAHGNSLRALAKHIEGISDADIMDLEIPTGQPLVYELDDN
                     LKVISKRYL"
     gene            complement(16624..17181)
                     /gene="frr"
                     /locus_tag="PXD81_000792"
     CDS             complement(16624..17181)
                     /gene="frr"
                     /locus_tag="PXD81_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HDL5536842.1"
                     /translation="MINEIKKDTQDRMEKSLEALKGHIAKIRTGRAQPSLLDGIQVEY
                     YGSATPLRQVANVVAEDARTLAVNVFDKSLISAVEKAILTSDLGLNPSSAGTTIRVPL
                     PPLTEERRRDLIKIVKAEGEQGKVAIRNVRRDANDQIKALLKDKEISENEQHKAEEDI
                     QKITDTFIKKVDEVLAAKEKELLDF"
BASE COUNT         4857 a         3808 c         3204 g         5323 t
ORIGIN      
        1 gtaatgtccc ttttttgaat gtctactaca aaatgggata ttttacaaac cgattgacta
       61 atgaagcaag aatataatta actacaactg gatatatttt gcaaatttcc cgataaattt
      121 caccgcttgt agataaaaac cgtagataaa aaccatagat aaaaaatagg agctaaacat
      181 ttttagcccc taccgtcatc agaaataaat cccgactaaa atcgccacga acacacctaa
      241 gccgaaatag tggttattta gaaacgcttt aaagcattcg ctacgttctc tttttttggt
      301 cagccaacat tggtagataa acagcgtggc acttaaggct aatacgacaa aatagccgat
      361 gtgataatct tttaccacgc ccaataaccc caataaaatc aaggtgataa actgtaataa
      421 cgcaataatt ttattatcgt attgagcgaa taaaattgca gtggatttaa ctccaattcg
      481 taaatcatcg tcacgatcaa ccattgcata ttgggtgtcg taggcgactg tccacgctag
      541 attggcaaaa aacagcagcc agcattcaag tggaagagtt tccgttaccg caccaaatgc
      601 catcggaatc gaccaaccga atgccattcc aagcactact tgtggcaagt gggtaaaacg
      661 cttcataaag ggataaatca ccgccagaaa aactgccaca aaggaaagca gaaacgcata
      721 gcggtttagc attaaatcga gcagaaaagc gaacaacagc aagatggcaa aaatgacttt
      781 tgcctcagtg gtggtcactc tgccggtagc taatgggcgt tgagaggtgc gtttaacgtg
      841 tccgtcaatt tctctatcgg cataatcatt aatcacacaa cctgccgcac gcataacgat
      901 taccccaagg gtaaaaatgg ctaaaatcgc aacaggcggc attccctctg cggcaataaa
      961 taacgcccat agtgtggggt gtaataataa taacgtgccg atgggtttat cgaaacgcat
     1021 taactgggcg taggcaatcc atttttgttt agaaaaaaga gtttcgttat tcattacaaa
     1081 ttttgacgat aacgtccgat tggcttaaat gagtctgtaa ttctaacggt aaatcttgat
     1141 ctgtaaacaa ataatttact tctttaacat ttcctaaacg tgcaatagct gtacgggtaa
     1201 actttgaatg atcggtcact aaaaccactt gtcgggaaca ttccattaat gcacgcttta
     1261 cctgcacttc gtggtaatcg taatcgagca tagagccgtc catatccacc gcactaatgc
     1321 ctaaaatccc aaagtccaaa cggaattggt taataaagcg aaccgtttct tctccgatta
     1381 gcccgccatc acttcttagc cctccgcctg ctaccgtaat ttggcaatct tcattttgca
     1441 tcagaatatg agccgcattt aaattattgg taactacttt taaattttta tgcgaaagta
     1501 aggctaatgc cactgcttct gaagtcgtgc caatatctaa aaataatgat gctccatttg
     1561 gaatcagttt tgccacctgt tgggcaatcg catttttctc gtgtgagaaa aattgcttgc
     1621 ggtgggtgta atcactattc tccgtattgg aaggagcggc tgccccaccg tgatgacggc
     1681 gaatcagatt attttctgcc atttcattca gatcacggcg aatggtttgc ggactcacat
     1741 tcaattcagc cactaattgc tcggtactca catagccaag ctgattaacc agctcaatga
     1801 tctttttgtg tctgtttgcc tgtttcatat ttcctctttt gcaaataatt tgcagaaaaa
     1861 gaccgcttgc aagcggtctt ttttcagtta aattttacaa tttatcaaca agttcaaccg
     1921 catagccgat atagcttgcc ggtgtcatct ctttcaaacg atttttttca ttttccggaa
     1981 tttccaactt atcgataaac tcacgcattg cggtttcatc aacccgtttg cctcgagtca
     2041 gctctttaag tttttcatac ggtttttcaa tgccataacg acgcataaca gtttgaattg
     2101 gctctgccaa tacttcccag ttttggttga gttcatcacg caagtgttgt tcattcactt
     2161 ctaatttgct aatacctttt aaggtggacg cataagcaat taacgcataa cctaaaccta
     2221 cacctaaatt acgcaacacg gtggagtcag ttaagtcacg ctgccagcga gaaatcggca
     2281 atttttgccc caaatgagcc attaccgcat ttgccaagcc aagattgcct tcggaatttt
     2341 caaaatcaat cgggttcact ttatgtggca ttgtgctaga gccgatttca ccggcaatag
     2401 tgcgttgttt gaagtgattt aaggcaatgt agccccacat atcacgatca aaatcaatta
     2461 aaatcgtatt aaaacgagcc acgcaatcaa agaactccgc aatataatcg tgcggttcaa
     2521 tttgggtggt gtatgggttc cacgtcacac ctaacgattc cacaaattct tggctgaaag
     2581 tgtgccaatc aatctccgga tatgctgata aatgtgcgtt atagttgccc actgctccat
     2641 tgattttagc gagaatttct aaattttcta actgtttata ttggcgttgt aaacgatatg
     2701 ccacatttgc catctcttta cccattgtgg tcgggcttgc cggctgaccg tgtgtgcgag
     2761 aaagcaacgg aatatgttgg taacatttcg ccagctctgc tacagcatca atcacttttt
     2821 tccattcagg taataatact tcttcacgag cggttttaag cattaaagcg tgcgaagtat
     2881 tattgatatc ttcagaagta caagcaaaat gaataaattc attcaccgct tgtaattcag
     2941 gcaacgcttc acatttctct tttaggaaat actccaccgc ttttacatcg tggttggtgg
     3001 tacgttcaat cgttttaatc cgattggcat cttctaacga aaaatcagcc acaatttggt
     3061 ttaggtaatc gtttgctttt tcagaaaaca ctggaacttc attaatttgt gtatgcgatg
     3121 ccaatttttg taaccagcga acttccaccg tcacacggaa ttttaataag ccaaattcac
     3181 taaaaatcgg acgaaggctc gcaactttgt cttgataacg tccatcaatc ggggataacg
     3241 cagttaaagc ggtaagttcc attgttttat attctccaaa taggtaaatg ttcggttaga
     3301 tttgacgata aaatttctgt gcttggttaa aaattttctt gcgtgaaaat aaaaattgcc
     3361 aacggcttcc gccgacttgc tgccacaaaa taccggctcg aataccggct agtagcgagg
     3421 ctcgaatttt attctgtata tccgggcgaa ccaagtaatc ttgcattccc atcacgtgaa
     3481 ttttcgtacc taacgggctg ataatatcgc tataaatagc ggctaaattg gcaatcattt
     3541 gatctgccat cacattatct tcatacaggg ctaactggcg ttccacttgc tgtaaacgct
     3601 gggctaactg ttgttttgct tcagggtttt tattgagttt ttggctaagg gcgagcaggc
     3661 ttatccaata acgtccgact tcggtatcta atttgccgtt accgccgcca gtttgagcta
     3721 atgcggtttc taagcccatt tttaaatggg aaagatcatc gccaaataca cttaacgttg
     3781 attccggctg agtgattaac aggctttcta aagcacactt aaaagcctct ctgtctgcca
     3841 tgcctttatg tgcaaattgt tgcactaacg tcgccgcttg gcaaacgccg gcataagcaa
     3901 gggtaatatc gtggtaatcc gccatctctg taaaattcaa tatgataaaa cattagtggg
     3961 aatatagcat tttttcagct tcttttgaat ggcaaaaggc atttattcta ttgaaaaata
     4021 aatgcctttt cattaatacg caatttgctg caaaaactat cctaccaatc gcccattatc
     4081 tattcgataa accacatcaa atttatccag tccgctttgg cggtgagtga tcataattaa
     4141 ggtgcgatca cagcagtgag tcaaaattag gtttaaaatc tgctgttcgg tttctctgtc
     4201 cagtccttct gttggctcat caagtaagac taattcagca gaactcagga gcaaacgggc
     4261 aagccctaaa cggcgttgtt cgccaccaga aagcggtctg ccgccctcgc ctaaccataa
     4321 atttaagccg tctgtttcaa gcaaatgccc taagcctact tttcttactg cttcttgcat
     4381 ttgttcgtct gtagcttgag cattccccat taataaatta tcttttaggg tttggctaaa
     4441 aatatgcact cgttggctta aggtaactaa ttttgagcgt aacatcggct ctgaatattc
     4501 gtcaatttta caattattca gccaaatttg accgcttgta gggtcgtagt tgcggtttaa
     4561 cagttggaat atggtcgatt ttccgctacc cgttttacct aagattgcca ctttctgccc
     4621 ttttaatacc ttaaaggaaa gattatttag tacgttttgc tcgccgtaat aagcaaagct
     4681 cacgttttca cagcgtacaa gcggttgatt ttggctcaaa ttttgccatt ctgccttgct
     4741 accaaatgtt acattaggct gttgttcggt aatttcacta atccgctcgg cggcggtaat
     4801 cacttgccct aaatgcaaaa aggcgatacc aatcggggaa agaatctccg ccgatgccat
     4861 tacacagaaa atcactaacg caattaaggc ttcagggtat tttgcagtgg gcacattaat
     4921 cgaagttgaa accaaataga tcaccacaca agtcaaaatg ccgttggata acattatcaa
     4981 gctatttgat aagccggaca gtcggctttc tttgctttga gcgttcagcc attgttgttc
     5041 agtttgctgt agtttttcgc tcatttggtt caagttgcca aagagtaaaa attctgcatt
     5101 taactgtacc cactcaataa attggctgcg gtaatttgct cggctttgta ttgcattacg
     5161 ccctaatttc aaacccaatc gataaaatac ggtaggaata acggcaagca ataccaatag
     5221 tgtgccgcaa atgatcagca ttaatggcac ggaaacaaaa gaaagcccga ttgccataaa
     5281 tgcaataatc atcactgcac tgacaaaagg cgagactaaa ttcagatata gggtgtctaa
     5341 cgtatccaca tctgccacca agcggtttaa caattcgctg tttctaaagc gatttagccc
     5401 gcttggactc agtggaatca attttttaaa gacgctagca cgcaaattcg ccaatacccg
     5461 aaaagtcgca tcgtgggtga ctaaacgttc aaagtaacgt gaaattgtcc gccctactgc
     5521 caaccctctc acccccgatg aagggtagaa aaagttaaaa ataagcgcat tgccggctag
     5581 aaatgaggca gctaaaaacc aaccggaaag gctgagcaaa ccgatacttg ccgctaatcc
     5641 taaaattgcc agcacaacac ctaataatag tcgcccaaaa tgggtttggt agagagcaaa
     5701 aaacgggaac aacgatttca ttagatttct ccctgatgca ctaattcatt atgtattaat
     5761 tccgcaaaaa agccggtgtg ttctaattca gtgaaacgac ctttctgaat gatttgccct
     5821 tgtttcatca cccaaatttc atcgcattgg gttaaatctt ccactcgatg agtaatcatt
     5881 agcgttgttt gctctcggct gagattatgc agagcggcta acacttgctg ttccgaatcc
     5941 atatcaaggc ttgcagtcgg ctcgtctaac agtaataatt cgtacgggcg aagcaacgct
     6001 cgtgcaatcg caatccgctg tgcttgcccg ccagagatac cgatattact gtcttgcact
     6061 tgatgctcca accctaaacg tgccacaaat tcatcggctt ttgaaagctg taatgccttg
     6121 gctaattcag aatctgtcgc attagggttg cctattaaaa tattctcttt caaactgcca
     6181 tgcattaatt gcggattttg ccccacccac gctaatttcg ctcgccattg gcttaaattt
     6241 aaatctcgca gttcggtgcc gttaatcgta accgaccctt cataaggcaa gaaaccaagt
     6301 aacatattca ttaatgaact ttttcctgca ccactttgcc caaccaaagc gatatgttgg
     6361 tgtgcattca actcgaaatt taacgcttgg gttaaggctt tgccttgtgg tgagaagatc
     6421 acgcaatctt tggcttgaat cacaagcggt tgattttcaa cattttcttg caaattgcca
     6481 ccttgcgttt tcaccttttc ttttaagaaa gtttcgatat tatctgcggc actaatcgct
     6541 gctgccttgt cgtgataaaa cacgccaagt tcacgcatcg gttggtaaaa ctcaggtgcc
     6601 accattagac aaaagaagcc gataaataag gtcacgcccg taccataata gccaaaatcc
     6661 agctcaccta aaaaaataaa gccaaaatag accgccgtca ccgcaatcga taccgccgtg
     6721 aaaaactcca ataccgcaga ggataaaaac gccattttta gtacgtccat cgtactgatt
     6781 cggaaatctt cggtgctttg gtagatttgt tcagtctgct tttccgcttg cccaaataag
     6841 cgaatggttt ctaaaccttt taatttatct aagaactgtc cgctaatacg ggaaagaatg
     6901 ccgatattac gctgatttgc ctccactgct ttcattccgg ctaaaatcat aaataaaggt
     6961 aataatggca ttgttgcaaa taaaatagcc cctgctgccc agttaatcgg gaatacaaaa
     7021 cagaggatca gtaatggcac tatcagcgat aaaaattgct gtggcaaata gcgtgcgtag
     7081 aaattatgta gattttccac ctgctcgagc attaaggttg cccagctgcc cgccggtttt
     7141 tgttgaatgc tcatcggccc gactgcttcc attttggcta gaatttggcg gcgaagatgt
     7201 aaacgcaagg tttgccctgc tttgaaaccg acacgctcac gcagaaaaat gaagaatgct
     7261 ctggcagcaa aacaacagaa taagagcata atttcaacca gaaagctatt cggtgactgc
     7321 tttccgataa gcattttgtc taacattgtg gcaatcagcc acatctgtcc aatcatacat
     7381 aaactactga aactccctaa cagtatgttt aagtgcatat attttttgat gactttttgc
     7441 tgacttttaa gccatttttg taattcgcgt tggcgttgtt tttccataat tttgtcaatt
     7501 tttttgtttt ttcaaccgct tgtaaatcgg gtttgccatt ctactataaa gcaaaaaggt
     7561 tgttaatggc gaaacattaa caacctttca ttcagcctta actagaatag ctcgtcaaat
     7621 tcaaagcgat acattaaatc taccgcttgg ttaatacttg agacccattg taagtacaat
     7681 cttggcgtta aattatagcg taaggttaat tcggtaagcg gtgcaaaaat gcccacgcca
     7741 tacttcactc tgaatttagg ggcaaggctg gcacttactt ctactttggt gttatcgcca
     7801 atacctgcgg tggtcacgct taaatctttg agtccgaaag tgctaccaac atcacccacc
     7861 aatttactgc tttttgataa gctcatactg agtaatgccg ctgcgactgc attactggag
     7921 ctggtatcgc cactgctttc caatgaacga ccagttaaaa tataggaaag tgcttcattt
     7981 tgcgacattg ccggatcaga gaatactttt accgttggat tgtctgccaa accggtcact
     8041 cttaccccag ctgtaatggt cggatcttcc attgcctccg gattgcgtat agcttcaata
     8101 tctaaggttg gctgagacgg tgagccggca aaactaatat cacctttacg aatcagcaag
     8161 tcttgaccat aagcggcaaa tcgtccttca cgcaaatgaa cttgcccgta aagccctaaa
     8221 ccttgtttgc cttgcctgac agacaataag ccattcacgt tactgtttaa accataggct
     8281 ttgagtttga catcattacc gatgttaatc ttaatatctg cattaattgc cattccacct
     8341 gaggtttgag ccggaatatt acgttggcta aacggcactt tatttttcac agagccgtcc
     8401 ataatcacct catctccact tacactgacc gcactttcag gtaactgctc gacttcaatt
     8461 cttgcccaag ggatatctac atttccactt aaggttaaaa gagttggcgt tgccgtcact
     8521 tgaatatccg ggctaactgc cacttttgcg atattaggaa tatccacttg gaaacggtta
     8581 gcttgagcgt gaacccgtgt tttccacgca tttagatctc gccagtctgc atcaccctcc
     8641 aaacgcagtt cgctttgttt ggtttgtaat ttaccgctta aagtcgatgt tgccccgtgg
     8701 aaattcattg ctaaattccc acctgtaata tcaaacggca ttgtaacaga gcgagctttt
     8761 aactctgtaa cgtttagatt tccgtgcaat aacggagagg ttgcggtgcc gctcacggta
     8821 agacgagcat tcacattgcc atcaaccgaa tcgccccgtt caagtagcgg tcgaatcagc
     8881 tttaagctta attggtcaag atgaatattg ccggataacg cacgagcctt acttaaatct
     8941 ttcatcacta aatcagtcga aagtctgccg ttattttcga cccttaaatc agatttaagg
     9001 gttaaattat tgtcagacat tttcagatca attttaagcg gtgaaacggt gagtgggaaa
     9061 ctttttcccc cctccatttt ttgaataaat ttaatcgcat tagaggttaa atccagattc
     9121 acttgaggct gtttgttttt aaaccaagct gcatcgcctt tggcattcac aatccctgaa
     9181 agctggctgt ttttctccaa aaattgttgc aataccgcta aatcaaagtt acggatttca
     9241 aaaggcactt taccctcaac accggcatta aatgcagtcg ggaaacaaag attaattttc
     9301 cggttatgcc aacaatgtgc ggaaatattg gcgttaatgg ctttattgtc atatttcaca
     9361 ttcactgctt tatctgcttg gaattgacca aactcggtgg attgaatcga aacttggctt
     9421 aattgccctt cccaaatttc tttcaaacgg tcaaattttc cagaaatttg caaatcagca
     9481 ccaaccggat tgccttttgc acttaatttc aagctatgat tttcttcatt tccttttagc
     9541 aataaagtgg catttcccac tttgatttca ttataggcaa attgacgtaa ccccaaggtt
     9601 aaatcccctt gaatggtttt ctcagtggta attttgcctt ttgcggttaa attttgcagt
     9661 ttaaattgct catagcctac atttgaggcc actaaatcta aatcaatgtt aggttctgaa
     9721 accttacccg ataatttggc attaccattc acgctggctt ttaaattcgg cactaaccct
     9781 tgcaaattag gggctttaat atccgcatag aaatcagatt tttcgcctaa cacgcctttt
     9841 aaatcgatct tattttcacc atacaccagc gataagcccg gaacatctaa aagttgttga
     9901 tgatcggctt ttaaattacc gcttaagtgt agatttttct gtaataacga acctttaata
     9961 tcaatattcg agacgtccgc tttccatccg ctagctgaat tgccccgtcc tgcataacct
    10021 tcggaagata aagaaccgga caataccgcc ggccagtctg ccagtaggga tttagtgtta
    10081 atatgctgaa gtttcaaatt tgaacgccac gcaacgccct cgctccaatc cactaaaccg
    10141 tcgatcttcg ccgtaccttc cagcgtattg agacttaaat cttgtatatt aaaatgggtt
    10201 aattcccctt tgccagttaa actagccgag cttgcaggca cgcccattcc tttggcactg
    10261 actttagcct ccaaagcgta atccaggaga ttgccattaa gcctcatggc gatattctct
    10321 aaactcagca catcaccatt ttgctgagga gcaaacggat aacgcattga ctcactcttc
    10381 agcgataaat caaagggagt tttcgcctga gccagtgcaa cattgccctc caacgaggcg
    10441 ctaattgcac cagccgtctt cacttttagg ctggttttgt catatagctc accggaaaga
    10501 gaggcttcaa tttgattgaa cggcaatttt actttcgccg tttttgcctc gtgaccactt
    10561 agcgtccaac ttagcggata attatcttgt aacgataaag aaccgcttgc ggttagatcc
    10621 ccttggtcgc ttttgaaatc taaacttttt aggttgatat gctgatcttt tgcttgagcc
    10681 tgaagctgta aagaggcaac atttaacaat gagatcggct caatcaaatt ccctttttca
    10741 tctttagctt tttgagcaat cgctacattc gcaatatcaa tctgttcaat attgagatca
    10801 agaggcaaac taaagcgaat atttttatcc aataacggct gtgctaattg tgctttaatc
    10861 gctgcccaat ccactttatt atccacctgt tcggcctgtg caacagtttt tctctgtaat
    10921 tggtgaaagc cctctacgac tttttcactg gttttttcca ccgcctcttg aatcacttta
    10981 tcagcaatct cttgtggatt tgtcgctgat aacgcctctg tttttgcatt tttttgtgca
    11041 aaatcgaccg cttgttcagt atcagtttcg tctaacttta aggctaaaag cagatttagc
    11101 ccctctaact gtgtcgggtg aacggtaata gcccgccctt taccagaaag tgcagtgtgg
    11161 aaatgattaa gatgaatatc catatcatca actttgacct tgatattctc taaattgata
    11221 ttttgagctg aaatccccaa tggcaaattt aattcggtga aaggttcggt ctcttctttt
    11281 ggctgagaag ggggtaattt agtcgtatca atcaacacct ctgtatcttt aaggctgaga
    11341 ttattcaaac acatttcata gtggagcaag cagctaaaac caatatgcag atctgcttgc
    11401 ccaaccgtta cattcacacc gtccatcaca aatcgggcat cagaaagctg taacccatct
    11461 tgcaggctac cttgcacttg cccaagttgt aatggctcta cgaatttatc tacccagctt
    11521 aacactttac gttgcccgta acctgtgcat agaaagagaa tggcgagtaa aagtagaata
    11581 aacgtcagac aaaagccgtg cttcaaaaac cgccactttg attttttagg ctgtgttgct
    11641 tgtggctctt gtgctgagtc cgtttgattt gtggctgttt gtttattttc gtcagtcata
    11701 atcgtgcata tttatgaggt gataaaaagt aaacaagcgg tatgcgaaat tttactgaaa
    11761 ttcgaccgct tgttttcatt tacagatcgg aacctaaacc aatataaatt tgtattcctt
    11821 tggaattatc tggtgatttt accggcgtgg cgacatcaaa tttaatcgcc ccgataggtg
    11881 atgcccaacg cacccctaag cccgtaccgt agtagagatc ctgagttttg aatttattcg
    11941 atgccaagcc tgcatcataa aaaattgcac cccaccaatt agggtaaacc tgatactgat
    12001 attctactgt gcctgtggct aaatgagagc ctccgacacg tttgccatta ctcggatcac
    12061 ggggggaaat atccttatag ccaaagcccc gcacactcat atcgccaccg gcaaaatagc
    12121 gtaacgccgg tggaatacgt tcaaattgat tggctttcaa ataccccaac tccgccctta
    12181 aatagaaccg atggttatca aagtaagttc tcgcccaaga tgtcgaggct ttataactat
    12241 aaaagtttac atctgaacct actgcacgac tcccccagtt taccgttaat ttttgtgtat
    12301 ccccccataa tgggtaaggc ttgccatctg ttctggttcg attcaatgat gccgttgggt
    12361 aaactaataa ggttttaaat cgatcattag cttgagtaaa agaatcgtaa cgggctttta
    12421 aaccaacaga aaatgaccaa ccggtttcgt gagtccagaa acgttgtaag ccaagggtgg
    12481 cagcggttga ttttgtatcg ttttgatctt cgttttctaa agtgccggat aattggtaat
    12541 aataatgtag cggatcttct ctaagtggaa tattataacc aaattcaaat gtctgctccg
    12601 gcttagaaac gtacgttcgg ctttcaatac tatgacctcg gctatttaac caaggtttct
    12661 tccagttaag ctgtaacctt gggccaactt cagtttcgta gcctatccca atttccactt
    12721 cattgcgttt tttaggctga aataacacat ttaaatccac taatttatcc tgttcattta
    12781 aatgtggctc aaccatcact gatgaaaacc aattactgga aaggtaatct tgggttaaaa
    12841 tcgaaagatc gttagctaaa tatggctcac cggaatgaat tttaagaata ttttgcaaat
    12901 attcttcttt gatttggcta tcaacaaacc ggatttgtcc ataatggtat cgttttccgc
    12961 tatcatagcc taaacgccaa tccgctttat gatcggtcgg ataaacttcc aaacggtgat
    13021 aaagccaatg accgtcaaaa taccctttac taaaagcggt actttcaata tcacctttaa
    13081 aattatcata ggttttatgg tttaaacgac taccttgttt tggggcttta ctgactagat
    13141 gagcgaaatc ttcgtccgtt tttgcatcgc cttgcaaaac aatatcggtt tcatcaattt
    13201 ttaccgtctc tttatctagc tcaacattta atactaagag cggtttcttt ccctcttgag
    13261 gtagctgcac aaattggtat cggctattat aatagcccaa ggctctcaga gctttatcta
    13321 ctgtggtttg caccaaatat tgataacgct ctgaaccatc tgcatattca ttggcaattt
    13381 gatctaaata tagctccaca tttttcttgg catctttacc ctgaatgcct ttgacttcta
    13441 aagtgactaa tggctctatt tctggctcat catcactatc ttccgctaat ttttcttgca
    13501 gattttcaac tcgctcacga tctacgtcag acagcctttg ctcttgattt gcttgtgcca
    13561 ttgaagcaaa agccactaaa cagcagaatg ttaaaaaatg tagttttgta ttcattagta
    13621 acctttttat tcttttggaa tgccacgcaa aagcggaata ctgtcataag gcggaacaga
    13681 acttaacgct ggctcttgct caaagagcat aatgaatggt ttgtcaggca ctgttttttc
    13741 attacgccac aagtttccaa cactcaccaa ttcaatatct tgaggtagtt gtgctaaatt
    13801 ttgcttcagt acactaatcg tattcttgcg agggtgtcct atcaccaccg ctaccccgtt
    13861 ttttcgtgcg tgattaattg catgtgcaaa ctgtttttgt acatcagcaa gcagatcgct
    13921 atcatctaaa aaaatgtctc tctccagcgt attaatacca aattgacgag ccgttttagc
    13981 cgccacacta ctgccaatag ttttactgtc taaaaagaaa agcgtatttt cttttaacac
    14041 tttcaacaaa tgttgcattg tagcagagtc tgaagttgct ccactgccca tatggttatt
    14101 taaaccaata gcatatggta cttgaccacg agaagtatga ataagtttcc gaattttctc
    14161 ttcgttatca ccaatatgaa tacccccctc ttcaattggc tgtttcgatt taggttccat
    14221 tggcaaatga atcaaaatat cccgtttttg gttataagcg tcctttgccc gttctgttgc
    14281 atagggtgca acaggaataa tcgccacgct taccgccttg ggtaaggcta agatttctcg
    14341 atcttctttt attcgatagc ccacatcatc aattacaatc gccatttgag ctgcttgtac
    14401 aagcggtgta aaaagcacaa acatttgcaa aaaaatgtta aaattcaacc gcttgttaat
    14461 ttgtttaaac atctatttca ccaaatttag aggattaatc ggcgtgcctt tacgggtaac
    14521 accaaaatag agtgcagaac ggctttgtcc cccactgtta ccaaccttcg caataacctg
    14581 tccgccttgc actcggctac ccttgctgac tgaaatagat tggttatagc cataaaggga
    14641 aatatcacca ttgccgtggt caatcgcaac catattgcca taccctgcaa gccattgagc
    14701 cattatgact cgcccgctcg ccattgctct aaccgaagta ccggcagaag caccaatcac
    14761 tacaccgtgc caattattac caaaaggagt gataatattg ccagacaccg gcatcgtata
    14821 tcgaccagcc ttgatcggtg ttctctcaac attctcagtt gttttggact gaccggattt
    14881 tgcctgtttt tcccgctgtt gttcacgttt ttctgcttgc tgagcctctc tttgcgcttg
    14941 ttctaattgt tgttttagtg cctgttcatt gctgcgtaaa ttctctaatc ggctttgatc
    15001 ctgctcaagc gttttatcga ttaaacgtaa ggtactttca cgctggcttt gtactttctt
    15061 taaatctttc tcttgtttct tttgttcatt aagttgcgtt tgatgatctt tttgctggcc
    15121 tttcaattca tcacgtctgg ctttaagatc agcttgggtt ttacgaattt caacgatcgc
    15181 atcaatgcga atattattca tatgctgata ataagcggtc attctttccg cattttttgc
    15241 ctcttcagac attaaacgct ctaagaccga agggttcacc ccagaacggt aggcagagtc
    15301 caactgctct tttaattttt ctttctgctg tttttccaac ttttctaaac gggcaatttc
    15361 agtctcagtc gctttaatgg tctggcgaac ttccgttaaa gacatttcgg ttttttgtaa
    15421 tttatccaac accttcccca tttctacttc ttggtttttt agggtagatt gcaacgcatt
    15481 acgtttttgc ttttgttcat taatttttga ggtttgctgt tggatttttt gctgaatctg
    15541 attaagatta tttgcaaaag aaaaaggggt agaaaagctt attaccccac tgaaaatagc
    15601 agtccaacag taagaattta ttcgtttcac cagcatacct ttgtttcttc aaaatattat
    15661 taaggttata ttctaatcgg ttatttgctt aagatcagct ttttagtgaa agaatttaac
    15721 tatctatttc aaaatataaa gaattttgct ataaatagca caatttcaaa tccattaatt
    15781 ttactcaaag ctaaggagtc attatgcaat tagtatttat ccgtcacggt ttcagtgaat
    15841 ggaatgctaa aaacttattt accggttggc gtgatgttaa tttaacagaa cgtgggattg
    15901 aagaagcaaa atctgccggt caaaaattaa aagcagccgg ttttgaattt gatattgcgt
    15961 ttacttcggt tttaacccgt gcgatcaaaa cttgtaacat cgtattagaa gaatcagacc
    16021 aattatggat ccctcaagtg aaaaactggc gtttaaacga gcgtcactac ggtgcattac
    16081 aaggtttaga caaaaaagaa actgctgaaa aatatggtga tgaacaggtt cacatctggc
    16141 gtcgttctta tgacatctct ccaccggatt tagacccaca ggatccaaac tcagcacata
    16201 acgaccgccg ttatgctaac ttaccaaaag atttaattcc aaatgcagaa aacctcaaaa
    16261 tcacccttga gcgtgtgtta ccattctggg aagatcatat tgcacctgca ttaatttcag
    16321 gtaaacgcgt attagttact gctcacggta actcattacg tgcattagca aaacacatcg
    16381 aaggcatttc tgatgcagac attatggact tagaaatccc aaccggtcag ccattagtgt
    16441 atgaactaga tgacaactta aaagtaatca gcaaacgtta cttataattg gtaaatttcc
    16501 gataaaaaat aaccgcttgt agtgcaacac attacaagcg gttatttttt gttaattttt
    16561 tgcaaatggc aaagaacaaa aataaaggcg aatcatacat tcgccttatt gttttaaagt
    16621 agattagaaa tctaacaact ctttctcttt tgctgctaat acttcatcaa cttttttaat
    16681 gaaggtatcg gtgatttttt gaatatcttc ttcagctttg tgttgttcgt tttcgctgat
    16741 ttctttatct tttaataacg ctttgatttg gtcattagca tcacgacgaa cattacgaat
    16801 tgccactttg ccttgctcac cttctgcttt aacgattttg attaaatcac gacgacgttc
    16861 ttccgttaat ggtggaagcg gaacacgaat ggttgtgccg gcagaagatg ggtttaaacc
    16921 taaatctgag gttaaaattg ctttttctac cgcagagatt aaagatttat caaatacgtt
    16981 taccgctaaa gtacgggcat cttcagctac cacgttcgct acttgacgta atggagtcgc
    17041 agagccgtaa tattccactt gaataccgtc taataaagaa ggttgggcac gacctgtacg
    17101 aattttcgca atatgccctt ttaaagcctc aaggcttttt tccatacgat cttgggtatc
    17161 tttcttgatt tcgttaatca ttgatttttt at
//
LOCUS       DANLYI010000032        17149 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.032, whole genome shotgun sequence.
ACCESSION   DANLYI010000032 DANLYI010000000
VERSION     DANLYI010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 17149)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17149)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17149
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.032"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            complement(<1..233)
                     /locus_tag="PXD81_000793"
     CDS             complement(<1..233)
                     /locus_tag="PXD81_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HDL5536843.1"
                     /translation="MNELYEKYKDEPSMLVAIADGEGNIGVLEDIFLYKKQLAFSYVT
                     PLERWNWLHQCNLNSYAPAPLSVIEFYIQNGVE"
     gene            complement(235..9879)
                     /locus_tag="PXD81_000794"
     CDS             complement(235..9879)
                     /locus_tag="PXD81_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemagglutinin repeat-containing protein"
                     /protein_id="HDL5536844.1"
                     /translation="MNKHCFRTIFSKTLQRIVVVSELAKLAGKSESESMTSGAILQKI
                     CKIRPLAFSLFCALGFVTLSDNAMAETLIIQADSTAPKNQQPIILQTANGLPQINIQT
                     PNDKGLSHNKYSQFDVAEKGAILNNSRTNTATQQAGMVQGNPYLARGEAKVILNEVNS
                     SKPSVMKGYVEVAGKKAEVIIANPSGLHCDGCGIINADRATLTTGKPQINQGNLEGFV
                     VEKGKVTVAGKGLDNSRVDYTDILAREAKINAGVWSKKETKVITGKNTVKRSNSDNNL
                     QIIHTNQPLAGEEKPTVAIDVGELGGMYAGKIHLIGTEQGVGVRNAGHIGASADTLTI
                     DSQGRIVNSGTFNAQRQVQLNAQHGIDNQGKIENKQGNIQLNSKADIQNSGSIVARGG
                     NIQKKAETKIQQSGETVAKGNITYTARQIHADKNSLIAAGVDVTDTAQGEVRKLEAQS
                     VAGKTIQLTAPEKTTVQGKNIASGKINVAAAEIDANQSQNNAHTVEYSAKSGDIQANS
                     AQFSANQIKLTTPKWLSTEGSQLTAEVIEAQQANLNARGSVWKQTGEQDFRLKAESIH
                     TDSARFSVGGHFEIDGNRLTNNQGLLSSGKRLGLNLNDAVDSVDGKLVAKGELSIRAP
                     LLNNQQGLILSGEKQAITVNTLKNQSGVITSQTEQTLDITSELQNQGGRISASGVAIN
                     AQSLDNREQGEISSVKELALTVSQQVDNRHGVIQSVGNLTLNSTLLDNQGGVLKSATD
                     ITLNVPTVLNNRISENGSLIEAGGSLTMNSFEVENQGTLARQPQPIQGIIASQFVLNS
                     TKQLNNEKGGIYIGSSARFTLSDSLNNRNGEILSWGDLAIFGGKSLTMTNTEGKVSAN
                     NGLEITAKALSGDGDVQAGDIRLNLQENFDITKDINANRSMIIQTEGDILNRKKLAAN
                     DRLQLTANQITNAQNARISSADTRLTATSTVYNEGLINSRSDNDNAQTVIKAQEIHNI
                     GTGNIYGDKVALGAERILNQDKDGTSATIAARQSLGLAAKVIENNTLVYEANKKSGSL
                     IYSGGEIAIGRQLNEQDTVIGNAGQLHNRSSIIEAEGRIQLNADKVANVNSHYRSELE
                     TTDRQTVNEHFILPRDKHNRGGSDVYDNGHLPTINVNQLRKGYYRKTWSYWQKYDEAN
                     LPVITDISQLNATTVLAKPYAQSCANDESVDCTLLPAGNYKKDNPVWTWFNLTPPAED
                     APEMTPEIIQAMNEEDLANQHLSEAEQEKLQKEQGQGIAPVVPARPIEPVRGSTETEA
                     QYQKRLQTYQAELKAYEAAEAAYQRYVLLKPLIDWEAKYGANIQALDEAIAKHNKANL
                     SQEYPHFWNLWIKERMVKENITKASLPAQILAGGDLTYQSDEFINDKSWVIAGKALNP
                     IGRSRIANLDDADAVRQDWELGRRQFSFTEYHGGTRRRHSRYDENDGPLARLKEVHKD
                     MNIFVELENTSPTAYTDYVDAKAANAVKPEGNQVERGNLPDAPNPPTMAIERIPSPNA
                     AAFEVRSMTVDTRLPNQGLYRINPQADSHVLIETDPDFTDKKRWLSSDYMFNALRHDH
                     NNVHKRLGDGFYEQRLIREQINRLTGRQFLGDYTDFDSQYKALMDAGITFAQKFNLRL
                     GITLSPEQVASLTSDIVWFETQTVRLPSGETVSVLVPKVYALARKGDIDGKGSLISAD
                     ALTINSKEIVNQGTIAGRRLAQFNAERLENSGTLSGGVLAAKVSGDLENIGGKIEADK
                     AILLDIAGNFNHTSTTRTTQIDLDGFKRAETNLDRKALLHVKDENGLLQVQAGNITSA
                     GADIINDGQGQTYLSAKNQLNLTALQVGFDEKMGGGNHYRNEALNDVVISRVQGKGDV
                     VVSGKNLYSEGADLDAKARLALLAENDMVLGSATRSRDYEEYHKTKSGGALAKKTKTT
                     YDNISETEHKGTELTGEDILVKAGHDIQGESLLAVSRSGDIDIIGGNNVTLSSATNQL
                     SEQHIRETKRSGFLNGGEIGFTIGSQKTRQENSLEGAIQSTARNTLGSEGGNIRIQAG
                     NKASVSNVDVLISNEKTAVISGKNGVRVESGKDVINSTDNYEFSQSGLSIALSTPVTD
                     AVQSARQSVQKAKATQNEKLKGVYAVKAAEDAVIAAQNAQKVAETLGNLGNALTENHA
                     AAENPAVKISVSLGTQKQQRESRSQSVTHSKSTLNGGNIALISGAGKVALEGVDTRVK
                     DTLLLDGKLGIESKGVADTYQNNTKNKNHSASVGVFIGLDRDSFGIGFEAGVSGGKGK
                     ENIETETWQNNQLQAGKIVTNSANGKLMLDATNVKANHWEGEVQDFEARSRQDISKYK
                     SEYTEGSVHGTLAVGTTGVNGHVAYNGAKLNTAQVENQTAIDIGKSGMDVKVKKNAHF
                     EGAVMTSQAEKENNRFQAGTLTTGDIENHSELKTRSVAISGGTSGVNPMSALSLLGNK
                     NESERSTTKAAIGENIAITLTEDLNAETTLNHLNRDTQNANQKVTKYDISEVKETQEL
                     VKGIGEIAEKAYQIYTHSEREKVTEAKLELGKAQAQKASKEEIGKLEANLDRLQKEFD
                     KNYGTGSKTKRAVEAVTAALQGLAAKDVGQAAVGLASPYLNAEIKKYTEGDTQANLIA
                     HALLGAVEAAATGNNVLAGAAAGAGSEAAADFIVKTLYKGKSTQDLTEAEKQNVALLS
                     QLASGLASGLIGDSTQSAAVGADIGKRAVENNYLSNWQEQKRSQELKSCHGDARCENQ
                     VRLYWGLVDIGQDVSFVSGAVAGVPENLAESVEGLISMFSSPIETIVAMKELIAREDT
                     LSYIANMTKQEYLAKLDKMQVYYEQAGAVGSFNAGKEFGNMMTDVVTALTGAGALVKT
                     GVGTTAKIVTKSGKELAIPKTRSSASLGTCSFHGDMEVKTDKGYRQISSIKVGDKVLA
                     KNERTGITTYQKVQAHYSNPYDYTVYVEVADSKGKYHTIVSNKIHPFFTQVLSGRVPI
                     SSEGHSYNGEIAKAQWVDAQHLQKGYRLLAESGEWQTVTKVKIKQERLKAYNMTVEKD
                     HTYFIRGTNADNEGVWVHNDCWHALPNDAKQVKNIDGYKAYQFTDPKDGKVVTVVQKD
                     LNRFETPNHQAGVDPHFNRLSQQIDAETGRYLSTDNPVDGLYNRAYLRSETLQKIYAN
                     YEKLPNGNYRNKLTRQEIEQPIDIGHMYGWEHRRLSLAAKELNWSQKQFNDYVNARPE
                     NFRLENMSENRSHKNEMPGKHDIQRIKSDMEKFLKEGK"
     gene            complement(9931..11706)
                     /locus_tag="PXD81_000795"
     CDS             complement(9931..11706)
                     /locus_tag="PXD81_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
                     /protein_id="HDL5536845.1"
                     /translation="MKTPQNLILSTVLLSASFVAEANSSSLVQQARIDATQQQIDAAQ
                     QHRQQQYQQNQAEKFQPKADVRLETQINDTLTLSNHELPCYPIHQITLIDYSPDNSLT
                     TSQFQWAFEKAVKSFHLTLPHCFGGEGLAVLMKQIQNEIIGKGYVTTRVVTAEQDLRG
                     GKLVLTVIPGKIRNTLVVDSGKVPRFTKLHALTGFTFAEGDILNVRDIEQSLENLKRV
                     PTAEANIEILPSSSEKAEVGDSDLKISYSQAFPFRLNLGLDDAGSKSTGKLQTSATLS
                     IDNIFSANDLFYTSFTHSMKQKGDDNGRRASKNLTLYYSVPFGYWQLAFSHTHHRYHQ
                     EVFGAFDKSYLYAGESDTDKLTFSYLLYRDAKRKTSLSGSLWPRQSQNYIDGSEIEVQ
                     KRRMAGWEAGFSHKEYFPTATLELSANFKRGTAARGALSAPEEDYGEGTSRPKIITAS
                     ASLTKPFFWGKQPWQWQSSLNAQWNKTPLVPQDRFSIGGRYTIRGFDGELTLSGERGW
                     NWRNELSWQFNPNHAFYWAVDGGRVSGWATENQLGHHLMGTAIGFRGGVKGFSYDVFA
                     GRPVRKPEGFKTSDTVAGFNIGYNF"
     gene            11861..12946
                     /gene="queA"
                     /locus_tag="PXD81_000796"
     CDS             11861..12946
                     /gene="queA"
                     /locus_tag="PXD81_000796"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005601009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HDL5536846.1"
                     /translation="MLVSDFHFNLPDELIARYPTAERSASRLLHLNGETGEFADQQFV
                     DLYDHINEGDLLIFNNTRVIPARLYGRKPSGGKVEALVERVLDEHRCLAHVKASKAPK
                     EGAELIFGEDKLGEGKGFTATMVARHDTLFELVFNEEQPLFELLQQAGHMPLPPYIDR
                     PDEDADQERYQTVYSKVLGAVAAPTAGLHFDNAMLEKLKAKGVDTAFVTLHVGAGTFQ
                     PVRVETIEDHKMHAEYAEVSQEVVDKILATKAKGKRVIAIGTTSVRSVESAAQAAEKD
                     GKLIAPFFSDTAIFLYPGKTFKVIDALVTNFHLPESTLIMLVSAFAGYRNTMNAYAHA
                     VETKYRFFSYGDAMFINKNPNALSDVP"
     gene            complement(12973..13511)
                     /locus_tag="PXD81_000797"
                     /pseudo
     CDS             complement(12973..13511)
                     /locus_tag="PXD81_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015701733.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(13524..15806)
                     /locus_tag="PXD81_000798"
     CDS             complement(13524..15806)
                     /locus_tag="PXD81_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HDL5536847.1"
                     /translation="MTLDRFCIALILFHLPILWLPKAFLFVGIVIGTLVLILSVYLRS
                     KLTAVLGLLVCIGYIQIIQVAKNAEKTTASKTPVQFEISKILKQTDYQTAIATLNTGE
                     KIYLNWQSKQPLLLNQFYQAELALRPISARSNIGNFDRQRWYFANHINMTATVRKVET
                     LAKTDYSLRTNWLNKVKAETDALPTQGFLLALAFGERAWLKTEHWGQFQQTATAHLIA
                     ISGLHIGLAMLFGVYFAKGVQWLCLKSELTALQAVGFSLYFARIVSFITAFGYSYLAG
                     FAVPTARALLAIAFVLLCQVLRRHYTPWQYWGRIVALLILIDPLSLLSDSFWLSILAV
                     ASLILWYQYFPLSRFLNDEYRKKITKFNRLWLSLLHLQIGIWLIFSPVQLYFFEGISA
                     FALLANLIIVPLYSFLLVPLILFTLLTDNLFSTWLLADYLAQFSLWLLDPFSNAWFAL
                     SHWQQWQLLSLNLLVLVLLYCKLYRLSYKKWLSAVAVSLLFNLSFYLPKLFNQTQTEW
                     IMFDVGQGLAMAFIYQQNKAVIYDTGSSWQANDGSRNSMAKMEILPYLKRNGIEVEAI
                     FLSHDDNDHSGGVVELLQAYPKARLISSSKVTYHNINPEPCIAGTSWQFGEWQLLAIY
                     PSHIAERAKNQDSCVILAKNDRLKILLTGDSGVEQERQFAHQLGKIDFLQVGHHGSKS
                     STGETLLSITQPAFAVISAGRWNPWKLPNKQIIERLNRRHINVLNTVETGMIRVKFYP
                     SSIEIEQARGAFSPWYKQFY"
     gene            complement(15948..17138)
                     /gene="sstT"
                     /locus_tag="PXD81_000799"
     CDS             complement(15948..17138)
                     /gene="sstT"
                     /locus_tag="PXD81_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005610059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HDL5536848.1"
                     /translation="MLGGNLVLRIAIGLVLGVLLAFINKEWATSVGVLGQFFVKSLRA
                     IAPILVFVLVLAAIANKEVGSDSKLKPILVLYVLGTFFAALTAVVLSFMFPTTLELTA
                     SPDGLTPPQGIGEILKTVIFNLVDNPLVALSNANFIGILAWSIGLGIAFRHGSASSKA
                     FLNDLANAVSFVVKVVIAFAPIGVFGLVAETVAINGIDAFEDYARLLVVLVGAMLFVA
                     LVLNPLLVYWKIRRNPYPLTFTCLRESGVTAFFTRSSAANIPVNMNLAKRLGVRDEIA
                     SVAIPLGASINMAGAAITVTVLTLAAAYTQGIQPDFATALLLSVVASICACGASGVAG
                     GSLLLIPLACSLFNIPNDIAAQVIGVGFVIGVIQDSVETALNSSTDVLFTAAVSYAED
                     QKSA"
BASE COUNT         4408 a         4029 c         3415 g         5297 t
ORIGIN      
        1 atttccactc cattttgtat ataaaattca atcacagaaa gtggtgctgg tgcataagaa
       61 ttaagattac attgatgtaa ccaattccat ctttctaatg gtgtaacata agagaatgct
      121 aattgctttt tatataaaaa aatatcttct aatactccaa tgttaccttc gccatctgca
      181 atggcaacta gcattgaggg ttcatcttta tatttttcat ataattcatt catattattt
      241 accttctttt aagaattttt ccatatcgga tttgattcgt tgaatatcat gttttcctgg
      301 catctcattc ttatggctac gattttcaga catattttct aatctgaaat tctccggtct
      361 cgcattaaca taatcattaa attgtttttg actccaattt aattcttttg ccgctaatga
      421 taatcggcga tgttcccagc catacatatg acctatatca ataggctgtt caatttcttg
      481 acgggttaat ttattgcgat aattaccatt tggcaatttt tcataattag cataaatttt
      541 ctgtaacgtt tccgagcgta aataagctcg gttatatagc ccatctaccg gattatctgt
      601 cgataaatag cgtcccgttt cggcatcaat ttgctgagac aaacggttaa aatgtggatc
      661 taccccggct tgatggtttg gcgtttcaaa acgattgaga tctttttgaa ctactgtcac
      721 cactttcccg tctttcgggt ctgtgaactg ataagcttta tagccatcaa tattttttac
      781 ctgtttggca tcattcggta aagcgtgcca acaatcatta tgcacccata ccccttcatt
      841 atcggcattt gtacctctaa taaagtaagt atgatctttc tctaccgtca tattgtaggc
      901 ttttaatctt tcctgtttga tttttacctt agtgacagtc tgccactcac cactttcagc
      961 aagtaagcga tagccttttt gcaaatgttg agcatcaacc cactgagctt ttgctatctc
     1021 tccgttgtaa gaatgcccct ccgaagaaat cggcactctg cccgataata cttgcgtaaa
     1081 gaacgggtgg attttgtttg acacaatggt gtggtatttt cctttgctgt ctgcgacttc
     1141 tacatacacc gtataatcgt atggattact gtaatgagct tggactttct gataggtggt
     1201 tataccggtg cgttcatttt tcgccagcac cttatcgccc actttaatgc tggaaatctg
     1261 tcgataacct ttatcagttt tgacttccat atcaccgtgg aatgagcaag tgcctaaact
     1321 agcgctcgat ctggtcttag gaatcgcaag ttctttacct gatttggtga ctattttcgc
     1381 cgttgtccct acacctgttt taacaagagc accagcaccg gttaatgctg tcaccacatc
     1441 agtcatcata ttgccaaact cttttccggc attaaaagaa cctacagccc ctgcctgttc
     1501 atagtaaacc tgcattttat cgagtttagc taaatactcc tgtttagtca tattagcaat
     1561 ataggagagc gtatcttctc ttgcgatcaa ttctttcatc gccacaatag tttctattgg
     1621 gcttgagaac atggagatca atccttcaac gctctcagcg agattctcag gaacaccagc
     1681 aacggcgcct gatacaaaac taacatcttg cccaatatca actaatcccc aatataatcg
     1741 aacttggttt tcacagcggg catcaccatg gcaggatttt aattcttgtg agcgtttctg
     1801 ctcctgccag ttcgagaggt agttattctc cactgccctt ttcccaatat ccgccccaac
     1861 cgctgccgat tgtgtactat cccctatcaa acctgaagcc agtccgcttg caagctggct
     1921 tagcaacgcc acattctgtt tctcggcttc ggttaagtct tgcgtggatt tgcccttgta
     1981 gagggtttta actataaagt ccgctgccgc ttcgctccct gcgcctgcag cagcccctgc
     2041 cagtacattg ttccctgtcg cagccgcttc caccgcaccc agtaaggcgt gggcaatcag
     2101 gttggcttgg gtatcccctt cggtgtactt tttgatttct gcatttaagt atggtgaggc
     2161 taaacccact gccgcttgtc ccacgtcttt cgccgctaaa ccttgcaagg ctgccgtcac
     2221 cgcttccacc gctcgtttgg ttttactgcc cgtgccgtag tttttatcaa actctttttg
     2281 caagcggtct aaattggctt ctaatttacc aatctcttcc ttgctcgctt tttgagcttg
     2341 tgctttgccc aattccagct tcgcttccgt cactttttca cgctcactat gcgtgtaaat
     2401 ctgataagcc ttttctgcaa tctcgcctat gcctttcacc agctcttgtg tttctttcac
     2461 ctcactgata tcgtatttcg tcactttttg attggcgttt tgcgtatctc ggttgagatg
     2521 attaagcgta gtttccgcat ttaggtcttc ggtgagggtg atagcgatat tttctccaat
     2581 cgctgccttt gttgtgcttc gctcgctttc gtttttattg cccagtaagc tcagtgccga
     2641 catcgggttc accccacttg tccctccgct aatcgcgaca cttcgggttt tcaattcact
     2701 gtggttttca atatcgccgg tggtcagcgt gccggcttgg aagcggttgt tttctttctc
     2761 cgcttggcta gtcattaccg ctccctcaaa atgggcgttc tttttcactt taacgtccat
     2821 tccactcttg cctatgtcga ttgcagtttg gttttccacc tgagcagtgt tcaacttcgc
     2881 tccattatat gccacatgac catttactcc tgttgtccct accgccaatg tgccatgtac
     2941 gctgccttct gtgtattcgc ttttatattt gcttatatct tgtcgacttc ttgcctcaaa
     3001 atcttgtacc tcaccttccc aatgatttgc tttgacattg gttgcatcca gcatgagttt
     3061 accatttgca gaatttgtaa caattttacc cgcttgcagt tggttgttct gccacgtttc
     3121 agtttcgata ttctccttgc ctttacctcc actcactcct gcctcaaaac caataccaaa
     3181 actatctcta tctaaaccga taaatacgcc cacactcgca ctgtgattct tattcttagt
     3241 gttgttttga taagtatccg caacaccttt ggattcaata cccagcttac cgtcaagcag
     3301 cagggtgtct ttcactcggg tatccacccc ttccagcgca actttgcccg ctcccgaaat
     3361 caaggcaata ttgccgccgt tcagggtgct tttgctgtgg gtcacgcttt gactacggct
     3421 ttctctttgc tgtttttgcg tgccgagact gactgaaatc ttcaccgccg gattttctgc
     3481 cgcagcatgg ttttctgtga gtgcattgcc aagattgccc agcgtttctg ccactttctg
     3541 agcattttgt gctgcaatca cggcatcctc cgccgctttt accgcatata cccctttgag
     3601 tttctcgttt tgtgttgcct tcgctttttg caccgattgc cgtgccgatt gcaccgcatc
     3661 cgttaccggt gtactcaacg caatgcttaa gcccgactgg ctaaattcat aattatcggt
     3721 gctgttaata acatctttcc ctgattcaac ccgtaccccg tttttgcctg aaatcacggc
     3781 ggttttttcg ttggaaatca gcacatcgac attgctcacg ctcgctttat tgccggcttg
     3841 aatccgaata ttgccccctt cactgcccaa tgtattgcgt gccgtgcttt ggattgcccc
     3901 ctctaagctg ttctcttggc gtgttttttg gctaccgata gtaaagccga tttcaccacc
     3961 gttcaagaaa ccgctgcgtt ttgtctcacg aatatgctgt tctgaaagtt ggtttgttgc
     4021 cgaactgagc gttacattat taccgccgat aatatcaata tcgccactgc gtgagacggc
     4081 aagaagactt tcaccttgaa tatcgtgacc ggctttcact aaaatgtctt caccggttag
     4141 ttcagtgcct ttgtgttcgg tttcagaaat attgtcataa gtcgtttttg ttttcttcgc
     4201 tagcgcacca ccactttttg ttttgtgata ctcttcataa tcgcgagaac gtgttgcgct
     4261 acccaacacc atatcatttt cagccagtaa cgctaaacgc gctttggcat ccaaatccgc
     4321 tccttcactg taaaggtttt tccctgaaac caccacatcg cctttgcctt gcacccgtga
     4381 aatgaccaca tcattcaagg cttcattacg atagtgattg ccaccgccca ttttttcatc
     4441 aaaaccaacc tgtaaagcgg tcaaattcaa ctgatttttt gcagataagt aggtctgccc
     4501 ctgtccgtca ttgatgatgt ctgcgcctgc tgaggtgatg ttgcctgcct gtacttgcag
     4561 cagtccattt tcatctttaa cgtgcagtag ggctttgcgg tcaaggttgg tttcagcgcg
     4621 tttgaaaccg tctaggtcaa tctgtgtggt tcgagtggtg gaggtatggt taaaattgcc
     4681 cgcaatatcc agcaaaatcg ctttatccgc ttcaatcttg ccgccgatat tctctagatc
     4741 accgctcact ttggcagcaa gcacgccacc actgagtgtg ccactattct ctaaccgttc
     4801 ggcattaaac tgtgctaaac ggcggcctgc tatcgtgcct tggtttacaa tctctttact
     4861 gttgatggta agcgcatcgg ctgaaatcag gctgcctttg ccgtcaatat cacctttgcg
     4921 tgcaagggca tacactttcg gcactaatac cgataccgtt tcaccgcttg gcaagcgaac
     4981 cgtttgggtt tcaaaccaaa caatatcgct ggtcaagctt gccacttgct ccggcgataa
     5041 ggtaatgcct aaccgaagat tgaatttctg cgcaaaggtg atgccggcat ccatcagggc
     5101 tttatattgg ctatcaaaat ccgtatagtc gcccaaaaat tgtctgccgg ttaagcggtt
     5161 gatttgctct cgaattaagc gctgttcata aaaaccatcg cccaaacgtt tatgcacatt
     5221 gttatggtcg tggcgtaggg cattgaacat ataatcactg cttaaccagc gttttttatc
     5281 ggtgaaatcc ggatcggttt cgatcagaac atggctatcc gcttgagggt taatgcgata
     5341 aagcccttgg tttggcaaac gggtatcgac cgtcattgag cgaacctcaa atgcggctgc
     5401 attcgggctt ggtatgcgtt caatcgccat tgttggcgga ttaggggcat cgggtagatt
     5461 accccgttca acctgattgc cttccggctt cacggcattt gctgctttag cgtccacata
     5521 atctgtataa gcggtcggtg aagtattttc cagctcgaca aaaatattca tatccttatg
     5581 gacttctttt aaacgagcga gaggaccatc attttcatcg tagcgggagt ggcgacgtct
     5641 tgtgcctccg tggtactcag taaaagagaa ttggcgcctt cccagttccc aatcttggcg
     5701 aacagcatct gcatcatcaa ggttggcaat tctactgcga ccaatcgggt ttaacgcttt
     5761 gcctgcaata acccaacttt tatcgttgat aaattcatcg ctttgataag tgagatcgcc
     5821 acctgccaag atctgtgccg gtaagctggc tttggtgata ttctctttta ccatacgctc
     5881 tttaatccac aaattccaga aatgcgggta ttcttggctt aaattcgctt tattgtgctt
     5941 tgcaatcgct tcatctaatg cttgaatatt ggcaccatat tttgcttccc aatcaattaa
     6001 cggttttaat agcacataac gctgataagc cgcttctgcc gcttcatagg ctttcagttc
     6061 cgcttgatat gtttgtagtc gtttttggta ttgggcttcc gtttccgtag agcctctaac
     6121 aggttcaatc ggtcttgccg gaaccaccgg tgcaatgcct tgcccttgtt ccttctgtaa
     6181 tttttcctgc tctgcctcac ttaaatgctg attcgctaaa tcttcttcat tcattgcttg
     6241 aataatttca ggcgtcattt ccggtgcatc ttcagctggt ggtgttaaat taaaccacgt
     6301 ccatactggg ttatcttttt tataattacc tgcaggtaat agagtacaat ctacactttc
     6361 atcattcgca cagctttgag catagggttt tgccaatacg gtcgtggcat tcagctggct
     6421 aatatcggta attaccggca agtttgcttc atcatacttt tgccaatagc tccacgtttt
     6481 gcgatagtag cctttgcgga gttgattcac attaattgtc ggcaaatgcc cgttatcata
     6541 tacatcactt ccacctcgat tatgcttatc tcttggcaaa ataaaatgtt cgttcacagt
     6601 ttggcgatca gtggtctcca attccgaacg ataatgacta tttacattcg ctaccttatc
     6661 cgcattcaac tgaatacgcc cttcagcttc aataatactg ctgcggttat gtagctgccc
     6721 tgcgttgcca ataacggtat cttgctcatt taattgacgt ccaatcgcta tctctcctcc
     6781 gctatatatt aatgaaccgc ttttcttatt cgcttcataa actagggtat tattttcaat
     6841 tactttagcc gctaaaccga gagattgacg agcagcaata gtggcagagg tcccgtcttt
     6901 atcttgattt agtatcctct ccgcacctaa cgccacctta tcgccataaa tattccctgt
     6961 gccgatattg tggatttctt gagctttaat cacagtttga gcgttatcgt tgtccgagcg
     7021 gctattgatt aagccttcgt tatagacggt tgaagttgca gtcaaacggg tatctgccga
     7081 gcttattcgg gcattttgtg cattggttat ttggtttgcc gtgagctgca agcggtcatt
     7141 tgcggctaat tttttgcgat tgagaatatc gccctcagtc tgaataatca tcgacctatt
     7201 cgcattgata tctttggtaa tatcaaagtt ctcttgcaaa ttcaggcgaa tatcgcctgc
     7261 ttgtacatca ccatcaccgg ataacgcttt ggcagtgatc tccaaaccat tatttgcaga
     7321 aacttttccc tcggtattcg tcattgtgag tgattttccg ccgaaaatag ccagatcgcc
     7381 ccaacttaaa atttccccat tacggttatt taagctgtcg ctgagggtaa atcgtgctga
     7441 tgagccgata taaataccgc ctttttcatt attgagctgt tttgtgctgt tcagtacaaa
     7501 ctgcgatgca ataatcccct gtattggctg aggttggcgt gccaaggtac cttgattttc
     7561 cacctcgaaa ctgttcatcg taagtgagcc acctgcttca ataagtgagc cattttccga
     7621 gatccgatta tttaagacag tcggtacatt gagcgtgata tctgtggccg atttcagtac
     7681 cccaccttgg ttatccaaaa gcgtgctatt tagggtgaga tttcccacac tttgaataac
     7741 accgtgccga ttatctactt gttgggaaac cgtgagcgct aactctttta ctgaactaat
     7801 ttctccctgc tcacggttat ctaaggattg agcgttaatc gcaacaccgc tggcggaaat
     7861 tcgcccgcct tgattttgca gttcgctagt tatatcaagg gtttgctccg tttgagaggt
     7921 gatcaccccc gattgatttt ttaacgtatt gaccgtaatc gcctgttttt cgccactcaa
     7981 aatcagacct tgctgattat tcagcaatgg agcacgaata gataactcgc cttttgcaac
     8041 caatttgcca tcgactgaat ccacagcatc atttaaattt agccctaaac gcttgccact
     8101 acttaatagc ccttggttat ttgttaaacg attaccgtca atctcaaaat gtccacccac
     8161 tgaaaagcga gccgagtcag tatggatact ctctgctttt aggcgaaaat cctgctcacc
     8221 agtctgcttc caaaccgagc cacgagcatt taaattcgct tgttgggcct caatcacctc
     8281 tgcggttagc tggcttcctt cagtggaaag ccattttggg gtggtgagct tgatctgatt
     8341 tgcactaaac tgcgcactat tcgcctgaat atcgcccgat tttgcactgt attcaacggt
     8401 atgggcatta ttttggctct gattggcatc aatctcagct gccgccacgt taattttccc
     8461 cgaagcgata ttttttccct gcacagtggt tttttctggg gcagtgagtt gaatagtttt
     8521 ccctgccaca gattgagctt ccagcttccg tacttcacct tgtgcagtgt cggttacgtc
     8581 caccccagcg gcaatcagtg agtttttgtc ggcgtgaatt tggcgggcgg tgtaggtgat
     8641 attgcctttt gcgaccgttt cgccactttg ctggatttta gtttcggctt ttttctgaat
     8701 attgccaccc ctcgccacga tggaaccgct attttgaata tcggctttcg agttaagctg
     8761 aatattgccc tgtttatttt caattttgcc ttggttgtca atcccgtgtt gggcattgag
     8821 ttgcacttgt ctttgagcgt tgaacgtgcc gctgtttaca atccgccctt gactgtcaat
     8881 ggtgagcgta tcggcacttg caccgatatg cccagcgtta cgcacgccca cgccctgttc
     8941 cgtaccaatc aggtggattt tgccggcata cattccgccc agctcaccca catcaatcgc
     9001 aactgtgggc ttttcttccc ctgcaagcgg ttgatttgta tgaataattt gcaaattgtt
     9061 gtccgaattt gaccgcttga cggtgttttt gccggtaatg actttagtct cttttttcga
     9121 ccagacaccg gcgttgattt tcgcttctcg ggcgaggata tcggtgtaat ccactcggct
     9181 gttgtccagc cctttgccag caaccgttac cttgcctttt tccactacaa aaccttctaa
     9241 attgccttga tttatctgtg gttttccggt ggtcagcgtg gctcggtcgg cgttgataat
     9301 accgcagccg tcacagtgca agccgctcgg gttggcgata atcacttccg cttttttgcc
     9361 agccacttcc acataacctt tcattaccga aggcttactg gaattgactt catttaaaat
     9421 caccttcgct tcgccacggg caagatacgg gttgccttgc accataccgg cttgttgggt
     9481 ggcggtgttg gtgcggctgt tgttgagaat tgcccccttc tccgctacgt caaattggct
     9541 gtatttattg tgggaaagcc ctttatcgtt cggggtttga atattgattt gtggcagccc
     9601 gttggcggtt tgcaaaataa tcggttgctg atttttcggg gcagtgctat cggcttgaat
     9661 aatcaaagtt tccgccatag cgttgtctga taaggtaacg aatcccaacg cacagaacaa
     9721 gctgaacgca agcggtcgaa ttttgcaaat tttttgtaaa attgcaccgc ttgtcatact
     9781 ttcactttct gatttacctg ctaattttgc taattcagat accaccacaa tacgttgtaa
     9841 ggtcttgctg aaaatagtgc gaaaacaatg cttattcata ataattcctt aatagcacaa
     9901 tatacttaat acaatatgcc caatcattca ttaaaaattg taaccaatat taaaaccggc
     9961 aacggtgtct gaggttttaa acccttccgg ttttcgcacc ggtctaccgg caaacacgtc
    10021 ataactaaat cctttaactc caccccgaaa cccaatggct gttcccatta aatgatgtcc
    10081 gagctgattt tccgttgccc aaccggacac tcgaccacca tctaccgccc aataaaaagc
    10141 gtggttcgga ttaaattgcc agctcaactc attacgccag ttccagcccc gctcaccgga
    10201 aagagtcagt tcaccgtcaa atccacgtat ggtgtaacgc ccgccaatcg aaaaacggtc
    10261 ttgcggaacc agaggggttt tattccattg ggcatttagg ctactttgcc actgccatgg
    10321 ctgcttgccc caaaaaaacg gtttcgtcag gcttgcactt gccgtgatga ttttagggcg
    10381 agaagtccct tcgccataat cttcctcggg agcagataat gcccctcgtg ctgccgtacc
    10441 tcgtttgaaa ttagccgaaa gctcaagcgt agcggtcggg aaatattctt tatgcgaaaa
    10501 acccgcttcc caacctgcca ttcggcgttt ttgtacttca atttctgagc cgtcaatata
    10561 gttttgcgac tggcgtggcc aaagtgaacc ggacaagctc gttttacgtt tggcatcacg
    10621 ataaagcaaa taagaaaagg ttaatttatc ggtatcactt tcaccggcgt agagatagct
    10681 cttgtcaaag gcaccaaata cttcttgatg ataacgatga tgagtgtgtg aaaatgctaa
    10741 ctgccaatag ccaaacggca cggaatagta aagcgtgaga tttttactgg cacgtctgcc
    10801 gttatcatca cctttttgtt tcatcgaatg ggtaaaagag gtatagaaca ggtcatttgc
    10861 cgaaaaaata ttatctatag acagagtggc ggaggtttgt aatttgcctg tggatttcga
    10921 gccggcatcg tccagcccaa gatttaagcg aaatggaaac gcttgcgaat agctgatttt
    10981 taaatcactg tctcccacct ccgctttttc gcttgagctg ggtaaaatct caatatttgc
    11041 ctccgccgtc ggcacccgtt ttagattttc cagagactgc tcaatatctc gcacattcag
    11101 aatatcgcct tcggcaaaag taaaaccggt taaggcgtgc agcttggtaa aacgcggtac
    11161 tttgccgcta tctaccacta aagtattgcg tattttgccc ggaatcacgg ttaaaactaa
    11221 ttttccgcca cgtaaatctt gctccgcagt aaccactcgt gtagtgacat aacctttgcc
    11281 aataatttca ttttggattt gcttcatcaa cacagctaac ccttcaccac caaagcagtg
    11341 aggtaatgtc aaatgaaatg attttactgc tttttcaaac gcccattgga attggctggt
    11401 agtcagagaa ttgtcaggag aatagtcaat gagagtgatt tgatgaatgg ggtaacaagg
    11461 caattcgtga ttggaaagag taagtgtatc attgatttgt gtctcgagcc gtacatcagc
    11521 tttcggctgg aatttttctg cttgattttg ttggtattgc tgttggcgat gttgctgggc
    11581 agcatcaatt tgttgttgag ttgcatcaat gcgtgcttgt tgtacaaggc ttgaagaatt
    11641 tgcttcagca acaaaagaag cacttaacaa taccgtgctt aaaatcagat tttgtggggt
    11701 tttcataaga tcaaataagt aagacattca acgtttttag tctataaaaa tttatgttgt
    11761 tttgcaaact ccgttttatg gagctttata catatctgac gactttggtt ttttatgcta
    11821 ttatctccgc catttttata ctatagagaa tagaataacc gtgttagttt cagattttca
    11881 tttcaactta cccgatgagc tgattgctcg ctacccgact gccgaacgct ctgccagccg
    11941 tttattacac ttaaatggcg aaacaggcga atttgccgat caacaatttg tggatttata
    12001 cgatcatatc aacgaaggcg atctattgat cttcaataac actcgagtga tcccggctcg
    12061 cctttacggt cgaaaaccta gtggcggcaa agtagaggca ttagtggaac gggtgctaga
    12121 tgaacaccgc tgcttggctc acgttaaagc ctcaaaagca ccgaaagaag gggctgaatt
    12181 gatctttggg gaagataagc taggtgaagg caaaggcttt acggcgacaa tggtagctcg
    12241 ccacgatact ctgtttgaat tggtgtttaa cgaagagcaa ccgttattcg aattgctaca
    12301 gcaagctggt catatgcctc ttccacctta tatcgaccgc cccgatgaag atgctgacca
    12361 agagcgttat caaaccgttt atagcaaagt gttaggtgca gttgccgccc ctaccgccgg
    12421 tttgcatttt gacaatgcaa tgcttgaaaa actaaaagca aaaggggtgg atactgcctt
    12481 cgtaacattg cacgttggtg ccggcacttt ccagccggta cgggtagaaa ccattgaaga
    12541 tcataaaatg cacgccgaat atgccgaagt gagccaagaa gtagtcgata aaattctcgc
    12601 aacgaaagcc aaaggaaaaa gagtgattgc aattggtacg acatcagtac gttcggttga
    12661 aagtgccgcc caagcggctg aaaaagatgg gaaattgatt gctccgttct tttctgacac
    12721 cgctattttc ctttacccgg gaaaaacctt taaagtcatt gatgcgttag tcaccaattt
    12781 ccatttaccg gaatccacgc taattatgtt agtctcagca tttgccggct atagaaatac
    12841 gatgaatgcc tacgcccacg cagtagaaac taaataccgt tttttcagct atggcgatgc
    12901 gatgtttatc aataaaaacc caaatgcgtt gagtgatgtt ccttaattta tgagggttat
    12961 aagggaataa cattatgaac aaaaacaatc ttccaaccat tttaagggat tgttttcaat
    13021 atattcaata atttggaagt acgatgtttc attacgaata atgtgttcat aataattcct
    13081 ttgccaaatt tcagaaatat cggtattttg acgaacccat ttagttacgc caattttaaa
    13141 accacgcaca atagagccga ttgttcgtga tgttccccgt aagggcgaaa catttttcgc
    13201 ccttatacat ggggcaaatg atgatttgcc cctacagttt cgacgatttc aataatgcca
    13261 tgtaaatgat taggcataat cacaaagcta tgcaagctta catttggaaa atgatcgggt
    13321 atatcttccc aacattgacg tacaatattc cctatcttat taagcctcat ttcatcatca
    13381 acaatctcac cgagaaaatg gtgtttattt ttacaacaaa tagtaatgaa atagaatcct
    13441 tgtgatcggt aatcataatg ctttagacga attgaacgcc tcctatgaat ctccggctga
    13501 tatttctgca tttttcttat tccttaataa aactgcttat accaagggga aaatgccccc
    13561 cttgcctgct caatttcaat agaacttggg taaaatttta ctcttatcat tcccgtttct
    13621 acggtattta atacattaat atgtcttctg tttaatctct caataatctg tttattcggt
    13681 aatttccatg ggttccatct tccagcagaa atcactgcaa atgctggctg ggtaatcgat
    13741 aataaagttt cgccagtgct gcttttgcta ccgtgatgtc caacctgtag aaaatcaatt
    13801 ttgccgagct gatgggcaaa ttggcgttct tgttccacgc cagaatcccc tgtcagtaat
    13861 atcttcaagc ggtcgttttt ggctaaaata acgcaagagt cttggttttt agctcgttct
    13921 gcaatgtgtg acgggtaaat tgccaataat tgccattcgc caaattgcca cgatgttccg
    13981 gcaatacaag gctcgggatt tatattgtga tacgtcacct tgcttgaact aattaacctt
    14041 gcttttgggt atgcttgcaa taactccact accccacctg aatgatcatt atcatcgtga
    14101 cttagaaaaa tcgcctctac ctcaatgcca tttcgcttta aatacggcaa gatttccatt
    14161 tttgccattg aattgcgact accatcattt gcttgccaac tggagccggt atcgtaaatt
    14221 accgctttat tttgttgata aatgaacgcc attgccagcc cttgcccaac atcaaacata
    14281 atccattctg tttgggtttg attaaagagc ttagggagat aaaaactcag attgaacaat
    14341 agggataccg ctactgcact taaccacttt ttataagata gccgatataa tttgcaatac
    14401 agcaatacta aaaccaataa attcagtgaa agtaactgcc actgttgcca atggcttaat
    14461 gcaaaccacg cattagaaaa tggatctaac agccataggc taaattgagc aagatagtcc
    14521 gccaacagcc acgttgaaaa cagattgtcg gtcaagagcg taaataaaat aagcggtaca
    14581 agcaaaaagc tatacaacgg cacaataatc aggttggcaa gcagtgcaaa agcagagatg
    14641 ccctcaaaaa agtagagttg aaccggtgag aaaatcagcc aaatgccgat ttgtaagtgc
    14701 aaaagcgata accacaagcg gttaaatttt gtgatttttt tacgatattc atcgtttaaa
    14761 aatcggctaa gaggaaaata ttgataccat aaaattaaac ttgctaccgc taaaattgag
    14821 agccaaaaac tatcagaaag caggctcaat gggtcaatta aaatcagtaa cgccactatt
    14881 ctcccccaat attgccaagg ggtatagtgt cgccgcagaa cttggcaaag caagacaaac
    14941 gcaatcgcca gcaacgctct ggcggtcggc acggcaaaac cggctaaata gctgtaacca
    15001 aatgcggtga tgaagctaac tattcttgca aaataaagtg aaaatccgac cgcttgtaat
    15061 gcggtaagct cgcttttcaa gcaaagccat tgcacgcctt tggcaaagta aacaccaaac
    15121 aacattgcca gcccgatatg caagcctgaa atggcaatta gatgggcagt agcggtttgt
    15181 tggaattgcc cccagtgttc tgttttcagc cacgcccttt caccaaaagc cagagccaat
    15241 aaaaagcctt gcgtaggcaa agcgtccgtt tcagctttca ctttattcag ccaatttgtt
    15301 cgcaagctat aatcagtttt ggctaatgtt tccaccttgc gaaccgttgc tgtcatattg
    15361 atatgattgg caaaatacca acgctgtcgg tcaaaattac cgatattgct acgagctgaa
    15421 ataggacgaa gagccagttc ggcttgatag aattgattaa gtaacaacgg ctgtttcgat
    15481 tgccagttca agtagatctt ctcgcctgta tttagtgtcg caatcgctgt ttgataatcg
    15541 gtttgtttta agattttact gatttcaaat tgaacaggcg ttttactagc agtcgttttt
    15601 tccgcatttt ttgcaacttg aatgatctga atatagccaa tacaaaccaa taagccgagt
    15661 acagccgtta attttgagcg tagataaaca ctcaatatca gcactaaggt tcctattact
    15721 atacccacaa atagaaacgc tttaggcaac cataaaatag gcagatgaaa tagaatcagg
    15781 gcaatacaaa aacggtctaa agtcataatt cggcttactt tttaaagctg agtatgccgt
    15841 ttgtgcttaa tattttcaga ataaattttc agattttcga cacagttcgc aaaaatactt
    15901 gaaaaaataa aggcagactt tttagcctgc cttataaaga agagatatta tgctgatttt
    15961 tgatcttcag cgtaacttac tgctgcagtg aataatacgt ctgttgatga atttaatgcg
    16021 gtttcaaccg agtcttgaat cacaccaatc acaaaaccca ccccaattac ttgagcagca
    16081 atatcgtttg gaatattaaa taaactacag gctaatggaa tcaataataa tgaaccgcct
    16141 gccacacctg atgcgccaca agcacagatt gatgcaacta cgcttaatag tagtgctgtt
    16201 gcgaaatcag gttgaatacc ttgtgtataa gctgccgcta gggttaatac ggttacagta
    16261 atcgctgcac ctgccatatt aatacttgca cctaatggaa tagctacaga agcgatctca
    16321 tcacgaacgc ccaaacgctt ggctaaattc atattcaccg gaatgttggc tgctgaactg
    16381 cgggtaaaga atgccgttac accactctct cgtaaacagg taaatgtaag tgggtatggg
    16441 ttgcggcgaa ttttccaata cactaataat ggatttagta ctaatgccac aaataacatt
    16501 gcgccaacta agactactaa taaacgtgcg taatcttcaa acgcatcaat accattgatg
    16561 gcaacagttt cagctactaa accaaatacc ccaataggcg caaatgcaat cacaactttc
    16621 acaacaaacg aaactgcgtt agctaaatca tttaagaatg cttttgaact tgccgaacca
    16681 tggcggaatg caatccctaa accgatagac cacgcaagaa taccgataaa gtttgcatta
    16741 cttaatgcga ctaatggatt atcaactaag ttaaaaataa cggtttttag aatttcacca
    16801 atgccttgtg gcggggttaa accgtctggg cttgcagtaa gctctaatgt agttgggaac
    16861 ataaagctta aaacaacagc cgttaatgcc gcaaaaaatg tacctaatac ataaagcacc
    16921 agaatcggct ttaatttgct atctgaacca acttctttat ttgcaatcgc agccaaaact
    16981 aatacaaata ctaaaatagg cgcaatggca cgtagtgatt tcacaaaaaa ttgacctaat
    17041 acaccaacgc tggtagccca ttctttatta ataaatgcca gcaatacccc tagcactaaa
    17101 ccaatggcga tacgtaatac taaattaccg ccaagaattt ttgatgata
//
LOCUS       DANLYI010000033        17083 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.033, whole genome shotgun sequence.
ACCESSION   DANLYI010000033 DANLYI010000000
VERSION     DANLYI010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 17083)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17083)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17083
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.033"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            13..237
                     /locus_tag="PXD81_000800"
     CDS             13..237
                     /locus_tag="PXD81_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HDL5536849.1"
                     /translation="MVVKILKRKSQKTLQQIYTQPISANIKWTNIEALFLELGAEIEE
                     REGSRIAVVLFQQVRVFHRPHPNPDTDKGR"
     gene            280..609
                     /locus_tag="PXD81_000801"
     CDS             280..609
                     /locus_tag="PXD81_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HDL5536850.1"
                     /translation="MRNIMDINGYKAVIAYDPETELFRGEFIGLNGGADFYADNVIQL
                     KKEGELSLNIFLELCREKGIEPFKQYSGKFNVRLSPELHKAAVIAATAENLSLNEWIN
                     QTLEKSV"
     gene            complement(648..1034)
                     /gene="cueR"
                     /locus_tag="PXD81_000802"
     CDS             complement(648..1034)
                     /gene="cueR"
                     /locus_tag="PXD81_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005654127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HDL5536851.1"
                     /translation="MNISQAAEKTGLSAKQIRDYEKAGLLPPTSRTEAGYRFYNATDL
                     SRLHFISNARKVGFSLAQISELLKLNDNPNRSSRDVKQLTEQHIGELEQKIADLNQML
                     TLLKSWSSSCCGNANSECSILNGLNG"
     gene            1123..1323
                     /locus_tag="PXD81_000803"
     CDS             1123..1323
                     /locus_tag="PXD81_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HDL5536852.1"
                     /translation="MTITLQLDGLHCGNCVKSVEKALNGVAGVNQVSVTLEPQIAVVE
                     GSAEAQALIEAVEDIGFDAKLA"
     gene            1376..3568
                     /locus_tag="PXD81_000804"
     CDS             1376..3568
                     /locus_tag="PXD81_000804"
                     /EC_number="3.6.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005710411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HDL5536853.1"
                     /translation="MTSTAHYSPEQQLLIDGMHCAACVRRVEKALLKVEKVEFASVNL
                     ADQTAFVQGNANPEALVAAVVKIGFGAEVLESEEERRIKQQAQTQRVLGHKRTQFMLA
                     LVVGFALVAFGFYKGMTVNETNRSLWLSWAGISLATMYFSGKDFFLGAFSALKNRSAN
                     MDMLVAISTGTAWLYSCWLTLFPQPNAHVYFEASVMILGFINLGKYLELKAKQRSSLA
                     LEKLLDLAPKQAVIFEENIAKTIPAKAIKPQMRVQALTGDRLAVDGILDSGSIWVDES
                     MLTGEALPVEKKIGDKVRAGTLVQDGSGTYIAEQVGSQTALARVINAVRHAQSSKPPL
                     AQFVDKIAAVFVPVVVSIALLSALIWLAVGQDFAFALSIFTTVLIIACPCALGLAIPL
                     STIAGVARSAEFGVLVRNIEALQASSEIDTLVFDKTGTLTTGRMEVSDVLTFNGFEQK
                     RLLQLAKSVEQHASHPIAKAIAKFAENQTACGVTDVQVLKGLGISAKYEGSTIRMGNW
                     QFVGETIQANLASLLPAKEGDFSSKIFVSVDNQLAGVLSVSDQLRPEAKATIHQFQAE
                     GYQCVMLTGDRQQTADYFANALGLDGVIAEVLPEQKAEKIRALQAEGKKVAMIGDGIN
                     DAPALAQANVGIAMHNGSDIAVETADLSLMREGLAPLMQILPFSKKVLKNMKQSLLGA
                     FIYNVIAIPIAAGVLYPFTGWLLNPMIAAIAMTLSSITVVLNSQRLLK"
     gene            complement(3621..4406)
                     /gene="truA"
                     /locus_tag="PXD81_000805"
     CDS             complement(3621..4406)
                     /gene="truA"
                     /locus_tag="PXD81_000805"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011848489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HDL5536854.1"
                     /translation="MKIALGIEYDGSRYFGWQRQEAVESVQQQLEQALSVVANSPVEV
                     FCAGRTDAGVHGTGQVVHFETQANRPLQSWCFGTNAHLPDDIAVKWAVAVNEDFHARF
                     SATARRYRYIIFNQKLRSAILPKGVTHFHFPLDERKMHQAGQYLLGENDFSSFRAAKC
                     QSNTPWRNIHHLNVSRQGNYVIVDIQANAFVHHMVRNIVGTLLEIGQGRQPVEWAKWV
                     LEQRDREKAAPTAKAEGLYLVEVHYPEHFGIPKTPLGPLFLAD"
     gene            complement(4471..5160)
                     /gene="folE"
                     /locus_tag="PXD81_000806"
     CDS             complement(4471..5160)
                     /gene="folE"
                     /locus_tag="PXD81_000806"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HDL5536855.1"
                     /translation="MTTISPELTITENTLSPEAQKVRQALLAKGIETPTVTHTKDKAS
                     RRAEIQQHMQAVLELLGLDLSDDSLEETPHRLAKMYVDEIFSGLDYATFPKITNIENR
                     MKVSEMVLVDDITLTSTCEHHFVTIDGKVAVAYYPKKWVIGLSKINRVVQFFAQRPQV
                     QERFTEQILTAFQTILETDDVAVYVKATHFCVKCRGIKDTNSYTVTSAFGGVFLEDRE
                     TRKEFLGLLNK"
     gene            5261..5968
                     /locus_tag="PXD81_000807"
     CDS             5261..5968
                     /locus_tag="PXD81_000807"
                     /EC_number="2.7.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-D-manno-octulosonic acid kinase"
                     /protein_id="HDL5536856.1"
                     /translation="MQNIQYLFNADLIPEHQAEFVQSLLTCKNFDNSDRLLGFSKGRG
                     TTWFLDTSAELGINVVLRHYYRGGLFGKIIKDSYFFTRFEATRAYKEFFLLQQMLAWG
                     LPVPRPVAIKIERHFCCYRADIMLEKLENTQDLSKILQSHSLSNEQYEQIGKLIRSLH
                     NHQIHHSDLNIHNILLGNDGKFCLIDFDKCGIEPGENWKQNNLDRLLRSFKKEQGRLN
                     IFFSEENWQAVLRGYTL"
     gene            complement(6023..7120)
                     /gene="apbC"
                     /locus_tag="PXD81_000808"
     CDS             complement(6023..7120)
                     /gene="apbC"
                     /locus_tag="PXD81_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005617485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HDL5536857.1"
                     /translation="MNQLNEQQLVEIKAVLQQFSHPTLQKDLIALNAFKKAELGGDIL
                     RIEITMPFAWNSGFETLKAETEAKLKQVTGSSGVKWVLNYQIATLKRANKHPAVNGVK
                     NIIAVTSGKGGVGKSTTSVNLALALRAQGTRVGILDADIYGPSIPHMLGAKDQRPTSP
                     DNKHITPITAHGLQSNSIGYLMEEDNATIWRGPMASSALSQLLNETWWNELDYLVIDM
                     PPGTGDIQLTLSQQIPVTGAVVVTTPQDIALLDAIKGISMFEKVSVPVLGVIENMSMH
                     ICSNCGHHEAIFGTGGAEKIAQKYGTKVLAQLPLHIRLREDLDSGVPTVVAAPEHEIS
                     QAYLALAEKVASELYWQGSVIPSEIMIREVK"
     gene            complement(7117..8664)
                     /locus_tag="PXD81_000809"
     CDS             complement(7117..8664)
                     /locus_tag="PXD81_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HDL5536858.1"
                     /translation="MLKSLQAVTFVKKIAKVTACILLITSCDQLICPQSIQSVWVENR
                     QSDTLLKRAIYSAVFQLDPHKIKASADAAPLRDLLVGLTAYNRKGDVVPAIAQNWFTE
                     DEKEWLFILDEKAVWSNGQPVTAQDFVASWRRLIDPQNHSVLSKYLIYMGIENAKAIE
                     EQIKSVEELGVEALNAHTLRIRLNHPNHQLPEMLAHSALLPTYQGIAPTIDNFVSNAS
                     YRLEKLDAQKMVLKARTQEIPFQTIEYDLITTIQNCSAFDIVENPLLNKKTNIVKFPR
                     LCSYYYEFNFDDPLLAKREIREAIRTMVASANSGQEYGVPSQSVIPHSLVKDREKRWN
                     PVVVEQLLTKAGISPYNPLKLTLLYDENSINIANQVTRALSQSELFNITPKMVDWQTL
                     NKVREQKDYQIARAGWCADYPDPIVFLQKFHSMSPDNHSGYKSEIVDQKLNRLQTETL
                     SAEARESLIQEISEQLYSDVAVLPLFQYHHRVGIVSSLLGIDLNNDSEVIYSKDLKRN
                     LANKDNQ"
     gene            complement(8690..9340)
                     /locus_tag="PXD81_000810"
     CDS             complement(8690..9340)
                     /locus_tag="PXD81_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchE family NAAT transporter"
                     /protein_id="HDL5536859.1"
                     /translation="MDIIINFAIYLQFFIGLFAIVNPFGSLPIFFSMTAHQYEGERNR
                     TSLITAVSIGVILLVSLYFGNLILNAFSISLSSFRVAGGILIVSIAMTMISGKLGEHK
                     QNKEEKNEDITEYENIGVVPLAMPIMAGPGAIGSTIVWGTRYHTWEDYVGFSIAIIVF
                     ALICYILFRYSAPLVKKLGKTGSNVVTRIMGLILMALGIEIIVAGITTLFPVLLVK"
     gene            complement(9462..11333)
                     /locus_tag="PXD81_000811"
     CDS             complement(9462..11333)
                     /locus_tag="PXD81_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HDL5536860.1"
                     /translation="MIEKMHERTNGPVFKIIFALVSISFVIGGIGTGLISQDNSVAKV
                     NGEEISQQLFNNTLNREQNRLNAELGSRFWDLMDSPEYAAQFNQSVLNGLIDEELLRQ
                     YAKELKLGISADQIKSEIVNSQMFQQDGKFSNELYQQTLRHNNLSADGYAAIVQEGML
                     QSQLQEGIINSDFNVPAQQALLAKLLLQQREVRLAEFSVAKEMENQTASEQELRAYYE
                     ANKTKLLAPEKLAVEYVTLSPKDVESKVEITNEQIQTYYDRNKADYVTKGEAHLAHIQ
                     VASEAEAQAIEQALKNGEDFATLAKNKSSDTLSANNGGDLGWAKAGTFPKAFEDAVAN
                     LTAGQMSAAVKVDNAYHIIKVLERKPEQVIELAQVKDKIAQTIRQELVLTEYFTVARE
                     MANKAFENNSSLEEVAKAGNVELQKTDLFTRENVPTALQNEKVLKVLFEGELRQNKQN
                     SDALDVGNDANPQTLFVRVSDYDAERPQTFDEAKAAVENFVKAEKAEKALLAKVEENV
                     KALNEGKSVDVKFNAPQTLVYMQAEVENPVLAGTIFAMKKAQDKTTYQMARNQKGDVV
                     IVALDNITEGNAEQFKTLAPQFEQANRLILRNEFLQSLRAKASIEVNEDFMQQRSSH"
     gene            11515..11784
                     /gene="rpsO"
                     /locus_tag="PXD81_000812"
     CDS             11515..11784
                     /gene="rpsO"
                     /locus_tag="PXD81_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HDL5536861.1"
                     /translation="MSLSVEAKAKIVAEFGRDAKDTGSSEVQIALLTAQINHLQAHFA
                     EHKKDHHGRRGLLRMVSRRRKLLDYLKRTDLAKYSETIARLGLRR"
     gene            11856..12725
                     /locus_tag="PXD81_000813"
     CDS             11856..12725
                     /locus_tag="PXD81_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HDL5536862.1"
                     /translation="MAFASLFTLLDDIAAVLDDVSIMTKVAAKKTAGVIGDDLALNAN
                     QVSGKNVVPERELPIVWAVAKGSLINKVILIPLALLLSVFLPQAIIPLLMIGGAYLCF
                     EGVEKLLHRLLHKNNQAHSDETETLSEKDKIKGAIRTDFILSAEIIIIALGVLQDSST
                     LTQILSLSAIGVGITLFVYGLVGLIVKLDDIGLWLMQKKSTFSQAVGKGLLFAMPWFM
                     KGLSVIGTIAMFLVGGGIFTHNLPMIHHFVEQFGISPHFSTLVDFAVGIVVGIIACLI
                     ILPLMKLFSKKAH"
     gene            complement(12815..14080)
                     /locus_tag="PXD81_000814"
     CDS             complement(12815..14080)
                     /locus_tag="PXD81_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme biosynthesis protein HemY"
                     /protein_id="HDL5536863.1"
                     /translation="MFRTLFLMLLLLVGLISGPYLAGNQGYVRIETATTVIEMSIVML
                     VVFFVLAMAVVYLLEWIVTKICRMSNGAYNWFSTRKHKKAQQETLEGLMRMSEGNYSK
                     AEKLFSKNAKHADEPVLNLIKAAEAAQQNGDDLAANKYLIKATEIAGPNNVAVELVRT
                     RILLQQGKLPAARTAIDSLLELAPHSDEVLRLAIQIYKESRAYVALDRLLVEIGQRNF
                     LTAEEYAELEYFVDDGLQDERLQEEGQEGLLSWWENQPNRRRKSVYSRVGVIKRLIDS
                     DDHQSAQEIALETLKKFEDEQLQGLFVQLTRLQIDSDSKLLKVLAKRADKANVKYTDD
                     YARALGYIYTRDGQYQKAKSQFELLLAHDECVADDRIMALHVAEQMGDTLMANRIREE
                     NLKQVNMATQSKVNEPLALPQTATESNSL"
     gene            complement(14099..15409)
                     /locus_tag="PXD81_000815"
     CDS             complement(14099..15409)
                     /locus_tag="PXD81_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HDL5536864.1"
                     /translation="MSRSKKVTEHLEHSAEKVVEEAQQAVDSAGSFAKKEIEEDVVNE
                     AIEKDIQTEMQKAPKEQVVVQKSGGKGLALVALLVALAVGGAGHYMANKKFAEVESQI
                     QAVSAKANQPTTQTMVEMPNFENEKAQIAELVTEYQKALERIKALETAQNGYTQQISS
                     LQLQLQKLNNTSGSDNTFWLLSEADFLLNNAARKVVLDNDIETAKNLLLEADQVLSKA
                     PNTIHIREAIKTDLNTLTALHQIDQNALMQRVANLANKLDDLPILESEQASNSKQDGQ
                     VSDSLADWEKNLEKSAASFLDHFIRVSKRNAADEKAFVAPNQEIYLRENIRLRLQIAI
                     LAVPRQQSELYKKSLQTVGSWVRSYFETSNENVQHFLKELDELAEQTIYVDAPTSLQS
                     LKVIAQQLNKTPEKIEKVEIKAEKELEQAEPVKAEQDAMPLSAQ"
     gene            complement(15421..16185)
                     /locus_tag="PXD81_000816"
     CDS             complement(15421..16185)
                     /locus_tag="PXD81_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HDL5536865.1"
                     /translation="MNVLVTRLDSRGQELVDMLNEQQIFAIHQPLFTIEAGKELPLLP
                     SAISRLKSDDYVFVVSPNAVDYAAKTLVDTGFHFRSDLKYFAVGQRTAKYLTEKAEQA
                     VIYPIEFENSEGVLALPEMQNLTDKTILILRADSGRELLAETAVLRGATIQYLECYRR
                     EPVTDDIPEKISLCKRLGVDTIVITSSEILRSLYEQAKADCRAWLFECDLVVVSRRIA
                     KIAKQMGWQEDKIRVSDKADNVTLMNTLLFKVGKSN"
     gene            complement(16185..>17083)
                     /gene="hemC"
                     /locus_tag="PXD81_000817"
     CDS             complement(16185..>17083)
                     /gene="hemC"
                     /locus_tag="PXD81_000817"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HDL5536866.1"
                     /translation="LALWQANFVKSELEKHFPQLSVELVTMVTKGDIILDTPLAKIGG
                     KGLFVKELELALLENRADIAVHSMKDVPMSFPEGLGLAVICEREDPRDAFVSNNYHSL
                     EDLPKGAVVGTSSLRRQCQLMAKYPHLEVKSLRGNVGTRLSKLDNGEYDAIILASAGL
                     IRLGLQERIRSYISVEQSLPAAGQGAVGIETRANDERILNYIAKLNHSSTACCVMAER
                     AMNTRLQGGCQVPIGGYATLEGDELTLNALVGSLDGSQIIRASAKGHKQEAEQLGILV
                     AEQLLAQGADKILAAVYNENVQ"
BASE COUNT         4856 a         3693 c         3225 g         5309 t
ORIGIN      
        1 tggtactata ttatggtagt aaaaatccta aagcggaaat ctcaaaagac actacaacaa
       61 atctacaccc agcctatttc tgccaatatt aaatggacaa atatagaagc tctttttcta
      121 gagttaggag cagaaataga agaacgagaa gggagcagaa ttgcagttgt tttatttcaa
      181 caggtcagag tatttcatcg cccacacccg aatcctgata cagataaggg gcggtagcga
      241 gtattaaaaa atggttatct gaaaacggag tcattcctta tgcgtaatat tatggatatt
      301 aatggttata aagctgtcat tgcttacgat cccgaaacag aattatttcg aggtgaattt
      361 atcggattaa atggtggagc cgatttttat gccgataatg tcattcaatt aaaaaaagaa
      421 ggtgaactaa gtttgaatat ctttttagaa ctttgtagag aaaaaggtat tgagccgttc
      481 aaacaatatt ccggaaagtt taatgttcgc ctttcgcctg aattacataa agctgcggta
      541 attgcagcaa ctgctgaaaa tttaagctta aatgaatgga ttaaccaaac gttagaaaag
      601 agcgtgtagc ctaattgcaa aacaatcacc tattttgacc gcttgtatta cccgtttaac
      661 ccattcaata tcgaacactc cgaattggcg ttgccacagc aagagctact ccagcttttg
      721 agcaaagtca gcatttggtt taaatcggca attttttgtt caagctcacc gatatgctgt
      781 tcggttaatt gttttacatc tcggcttgag cggtttgggt tgtcgtttaa tttaagtagt
      841 tcgctgattt gagccagcga aaagcccact tttcgggcat tgctgataaa atgtaatcgg
      901 ctcaaatcag tagcattgta gaatcgatac cccgcttcgg tgcgacttgt cggcggcaga
      961 agtcctgctt tttcataatc tcggatttgt ttggcggaaa gcccggtttt ttctgcggct
     1021 tggctaatat tcattataat cctctcactt taatcgcttg accttaacct aaggttaagc
     1081 tttattatac gcctgaataa tcactcaaaa caggagaaaa tgatgacaat taccctacaa
     1141 cttgatggct tacattgtgg caactgtgtg aaaagtgtcg aaaaagcgtt aaatggcgtg
     1201 gctggggtga accaagttag cgtaacgtta gaaccgcaaa ttgctgtggt ggaaggctca
     1261 gcagaagcac aagccttaat tgaagcggtt gaagacattg gctttgacgc aaaattagcc
     1321 taattaataa ggggcagaaa ttttgcccta atctgcccaa tatttcggag gataaatgac
     1381 aagcacagct cactattcgc cagaacaaca acttttgatt gatggaatgc actgtgccgc
     1441 ctgtgttcgg cgagtggaaa aagccttact taaagtggaa aaggtggaat ttgcctcagt
     1501 caatcttgcc gatcaaaccg cttttgtgca aggcaacgcc aaccctgaag ccttggtagc
     1561 agcggtagtt aaaatcggtt ttggggcaga agtgctggaa agcgaggaag aacgccgaat
     1621 taaacaacaa gcccaaacgc aacgagtgtt agggcataaa cgcactcaat ttatgcttgc
     1681 ccttgtagtt ggttttgcct tagttgcttt tgggttctac aaaggaatga cggtaaacga
     1741 aaccaatcgt tcgctttggc tcagctgggc aggtattagc cttgcgacaa tgtatttttc
     1801 aggtaaagat ttctttcttg gggcattttc agcactgaaa aaccgctcag ccaatatgga
     1861 catgttagta gcaatcagca ccggcacggc ttggctctat tcttgttggc tcacgctttt
     1921 cccacaaccg aatgctcacg tttattttga agccagcgtg atgattctcg gctttatcaa
     1981 tctaggaaaa tacttagaac tgaaagccaa acagcgttcc tcgctagcat tggaaaaatt
     2041 gctggatctc gccccaaaac aagcggtcat ttttgaagaa aatattgcaa aaaccattcc
     2101 ggctaaagca attaagccac aaatgcgggt gcaagcctta accggtgacc gcttggcggt
     2161 tgatggtatt ttagacagtg gctctatttg ggttgatgaa tcaatgctca ccggtgaagc
     2221 cctacctgta gagaaaaaaa tcggtgataa agtgcgagcc ggcactctgg tgcaagacgg
     2281 ttcaggcact tacattgccg aacaggtcgg ctcacaaacc gctttggctc gtgtaatcaa
     2341 tgccgttcgc cacgcccaaa gcagcaaacc gccattggca cagtttgtag ataaaatcgc
     2401 cgctgttttt gtgccggtgg tggtttcaat cgcattactt tctgcgttaa tttggctggc
     2461 agttgggcag gattttgcct ttgcactctc gatttttact accgtgttga tcatcgcttg
     2521 cccttgtgca ttggggctgg caattccgct ttctaccatt gccggcgtgg cacgttctgc
     2581 cgaatttggc gtgctggtgc gaaatattga ggcactacaa gcaagcagtg aaatcgacac
     2641 cctagtgttc gataaaaccg gcacactcac cactggcaga atggaagtgt cggatgttct
     2701 cacctttaac ggatttgagc aaaagcgctt gctacaactt gctaaaagtg tagagcaaca
     2761 cgcctcacac ccgattgcga aagcgattgc aaaatttgcc gaaaatcaga ccgcttgtgg
     2821 tgtaaccgat gtgcaagtgt tgaaagggtt aggcatttca gcaaaatatg aaggcagtac
     2881 aatcagaatg ggaaattggc aatttgtagg cgaaaccatt caagcaaatc ttgcctccct
     2941 attgcccgct aaagaggggg atttttcctc aaaaattttt gtttcggtgg ataatcaact
     3001 cgccggtgta ttgtcggttt cagaccaact tcgccctgaa gcgaaagcaa ccattcatca
     3061 attccaagcg gaaggctatc aatgcgtgat gctgaccggt gatcgccaac aaactgccga
     3121 ctatttcgcc aacgcattgg ggttagacgg cgtgattgcg gaggtattac cggagcaaaa
     3181 agcggaaaaa attcgagcct tgcaagcaga gggtaaaaaa gtagcaatga ttggcgacgg
     3241 cataaacgat gctccagctc tggctcaagc caatgtgggc attgcgatgc acaatggttc
     3301 agatattgca gtggaaaccg ccgatctttc gctgatgcga gaagggctag caccactgat
     3361 gcaaattcta ccgttctcta aaaaagtgct gaaaaatatg aagcaaagtt tgctcggggc
     3421 ttttatctat aatgttattg cgattcctat tgctgccggc gtgctttacc ctttcaccgg
     3481 ctggctactc aacccaatga ttgccgccat tgcgatgaca ttgtcgtcaa ttaccgtggt
     3541 gttaaatagt caaaggttgt tgaaataatt taagcggtca aatttgcata tttttttgca
     3601 aatttgaccg cttgtaatca ctaatccgcc aaaaacagcg gcccaagagg tgttttcgga
     3661 atgccaaaat gctcagggta atgcacctcg accaaatata gcccctcggc tttcgctgta
     3721 ggggcagctt tttcccggtc acgttgctcc aacacccatt ttgcccattc cacaggctgt
     3781 ctgccttgcc cgatttcgag caatgttccc acaatattgc gaaccatatg atgtacaaag
     3841 gcgttggctt gaatatcaac aatcacataa ttgccttgcc gagacacatt taaatgatga
     3901 atattccgcc acggcgtatt agattggcat ttggcggcac ggaaagagga aaagtcattc
     3961 tcgcccagca aatactgccc tgcttggtgc attttgcgtt catcaagggg aaagtgaaag
     4021 tgggtaacac ctttaggtaa aattgccgac cgcagttttt ggttaaaaat aatatagcgg
     4081 taacggcggg cggtggcact aaaacgggcg tgaaaatcct cattcaccgc taccgcccat
     4141 ttcaccgcaa tatcatcggg taaatgagcg ttagtgccga aacaccagct ttgtagcggg
     4201 cggtttgctt gcgtttcaaa atgcacgacc tgccctgtgc cgtgcacacc ggcatcggtt
     4261 ctgccggcac aaaacacttc tacgggcgaa ttggcaacaa ccgacaacgc ctgctctagc
     4321 tgttgttgta cactctctac cgcttcttgt cgttgccagc caaaataacg gctgccgtca
     4381 tattcaatac ctaatgcgat tttcatttta ataccttgat aaaaagaggg gcgaaaaatt
     4441 tttcgccccc acagtaattg atatttgcaa ttatttattc aataagccta aaaattcttt
     4501 gcgggtttct cggtcttcta agaaaacacc gccaaaggca gaagtaactg tatagctatt
     4561 ggtgtctttt atgccacggc atttcacgca aaaatgggtg gctttcacat acaccgcaac
     4621 atcgtctgtt tctaaaatag tttgaaaggc agttaaaatc tgttcggtaa aacgctcttg
     4681 cacctgcgga cgttgggcaa aaaactgcac gacacggttg attttcgaga gtccaatcac
     4741 ccattttttc gggtaatagg ctaccgctac tttgccgtca atagtaacaa aatgatgctc
     4801 acaggtagaa gttaaagtaa tatcgtccac cagcaccatt tcactgactt tcattcggtt
     4861 ttcaatattg gtaattttcg ggaaggtagc ataatcaagc ccgctgaaaa tttcatcaac
     4921 atacattttc gccaaacgat gaggcgtttc ttctaagctg tcatcactta aatctaaacc
     4981 gagcaattct aaaactgcct gcatatgctg ctggatttct gctcggcgac tggctttatc
     5041 tttggtatga gtaacagtcg gagtttcaat gccttttgcc aacaaggctt ggcgaacttt
     5101 ttgagcctcc ggcgaaagcg tgttttctgt gatagttaat tcgggcgaga tcgttgtcat
     5161 tggttttcct ctcaaatgta agctgaagat tctaacaaat tgacatcaaa aaaaatagct
     5221 ctaaaatgag ctatttcagc cataaacacc ctctttattt atgcaaaata tacagtatct
     5281 ctttaatgcc gacctgattc ccgaacatca agctgaattt gtgcagtctc tcttaacttg
     5341 caaaaatttt gacaattccg accgcttgct aggcttctca aaagggcgag gtacgacttg
     5401 gtttttagat acctctgctg aactaggtat caatgtggtg cttcgccatt attatcgtgg
     5461 cggcttgttc ggtaaaatca tcaaagatag ttactttttt actcgctttg aggccacccg
     5521 agcgtataaa gaatttttct tactgcaaca aatgttagct tggggtttac ctgtgcctcg
     5581 ccctgtggcg attaaaatag aaagacattt ttgttgctat cgtgccgaca ttatgctcga
     5641 aaagctagaa aacacacaag atttaagcaa aatcttgcaa tcccattctt tatcgaacga
     5701 acaatatgag cagataggga aactgattcg cagtctgcat aaccatcaaa ttcaccattc
     5761 cgacttaaat atccataata ttttgcttgg taatgatggc aaattttgtc tgattgattt
     5821 tgataaatgc ggcattgagc cgggcgaaaa ttggaagcaa aataacctag accgcctgtt
     5881 acgctcgttt aagaaagaac aagggcgatt aaatattttc ttcagcgaag aaaattggca
     5941 ggctgttttg agaggatata ctctctaaga aacaataatg gcacaagctt agaaacttgt
     6001 gccatcaaat catcaatatt aactacttca cttcacgaat cataatttca gacggaatca
     6061 cactgccttg ccagtaaagt tcagaagcta ctttttccgc taatgcgagg taggcttggc
     6121 tgatttcgtg ttccggagct gccaccaccg ttggcacacc gctatctaaa tcttcacgca
     6181 gacgaatatg tagtggtaat tgtgctaaca ctttagtgcc atatttttgg gcgatttttt
     6241 ccgctccgcc ggtgccgaaa attgcctcat ggtgtccgca gtttgagcag atatgcattg
     6301 acatattttc aatcacccca agcactggta ctgagacttt ctcaaacatt gagatccctt
     6361 taatcgcatc taataaggca atatcttgtg gggttgttac taccaccgca ccggtcaccg
     6421 gaatttgctg tgaaagcgta agctgaatat cacccgtacc cggtggcata tcaatgacca
     6481 agtaatccaa ttcattccac caagtttcat ttaaaagctg acttaacgca ctgcttgcca
     6541 tcgggccacg ccaaatggtg gcgttatctt cttccattaa atagccgata gaattagatt
     6601 gcaagccgtg agcggtaatc ggcgtgatgt gcttgttatc aggagaagtt ggacgttggt
     6661 cttttgctcc caacatatgc ggaatagatg ggccgtaaat atccgcatct aaaatcccca
     6721 ctcttgtccc ttgtgctcgt aaggctaaag caagattaac tgaggtcgtt gatttcccta
     6781 ccccaccttt tcctgaagtg accgcaatga tattttttac cccattgact gccgggtgtt
     6841 tattcgcccg ttttaaggtc gcaatttggt aatttaatac ccattttaca ccgcttgaac
     6901 ctgttacttg ctttaattta gcctctgttt cggcttttaa ggtttcaaaa ccactgttcc
     6961 acgcaaatgg catcgtgatt tcaattctaa gaatatcgcc ccctaattct gcttttttaa
     7021 acgcattaag ggcaattaaa tctttctgta aagtcgggtg ggaaaattgt tgcaagactg
     7081 ctttaatttc aacaagttgc tgttcattta attggttcat tggttatcct tgtttgctaa
     7141 attgcgtttt aaatctttgc tataaattac ttcgctatcg ttattcaaat caatacccaa
     7201 aagggaggaa acaatcccaa cccgatgatg gtattgaaat aatggcaata cggcaacatc
     7261 gctataaagc tgctcgctaa tttcttgaat aagtgactct ctcgcttctg cactaagcgt
     7321 ttctgtttgt aaacgattta atttttgatc gactatctca cttttatagc cggaatgatt
     7381 atccgggctc attgaatgaa acttctgcaa aaatacaatc ggatcaggat aatctgcaca
     7441 ccagcctgct cgtgctatct ggtaatcttt ttgttccctt actttattta aagtctgcca
     7501 atcaaccatt ttaggtgtaa tattaaataa ttctgattga gaaagggctc gagtaacttg
     7561 gttagcaata ttaatgctat tctcatcata taacaaagtc agctttaaag gattataagg
     7621 gctaattcct gcttttgtta atagctgctc aaccacaaca ggattccaac gcttttctct
     7681 atcttttact aagctatgtg gaattacaga ctgactcgga accccatatt cttgcccact
     7741 atttgctgaa gccaccattg tccttattgc ttcacgaatc tctcgtttag ctaataatgg
     7801 atcatcaaaa ttgaactcat agtaataaga gcacaaacgc ggaaatttca cgatattcgt
     7861 ttttttattt aataaaggat tctcgacaat atcaaaagca gagcagtttt ggatagttgt
     7921 aattaaatca tactctatag tttgaaaggg aatttcttgg gttcgagcct ttaacaccat
     7981 tttttgagca tctagtttct cgagacggta agacgcattg ctcacgaagt tatctatggt
     8041 tggagcaatc ccttgataag taggtaataa tgcggaatgt gccagcattt caggcaattg
     8101 gtgatttggg tgatttagcc gaattctcaa tgtatgcgca tttaatgcct ctacaccgag
     8161 ttcttcaact gactttattt gctcttcaat cgcttttgca ttttctatac ccatatagat
     8221 gagatattta gacaatacag aatgattttg aggatctatt aaacgtcgcc agcttgctac
     8281 aaaatcttgt gcagtcacag gttggccatt agaccacaca gctttctcat ctaaaataaa
     8341 caaccactct ttttcatctt cagtaaacca attctgagca atggccggaa cgacatcacc
     8401 ttttctgttg taggccgtta aaccgactaa tagatctctt aaaggagcag catctgcaga
     8461 agctttaatt ttatgtgggt cgagttgaaa tacagcagaa taaattgcac gtttgagtaa
     8521 agtatcactc tgccggtttt ctacccagac agattgaatg gattgagggc aaattaattg
     8581 atcacaagat gtaataagca gaatgcaagc ggtaactttt gcaatttttt ttacaaatgt
     8641 aaccgcttgt aaggatttca acataaactc cccaaaggtt aattaagatt tattttacaa
     8701 gaagcacagg aaataaagtt gtaattcctg ccacaataat ttcaattccc agcgccatta
     8761 aaatcagccc catgatacga gtaacgacat tagatcctgt tttgcctaat tttttcacca
     8821 aaggggctga atagcggaat aaaatataac aaattaatgc aaatacaata atcgcaatgc
     8881 taaagccaac atagtcttcc caagtatggt agcgggttcc ccacacaatg gttgagccaa
     8941 ttgcccctgg tcctgccata atcggcattg ctaatggcac gacaccgata ttttcgtatt
     9001 cagtaatatc ttcatttttc tcttctttat tttgtttgtg ttcacctaat ttaccgctaa
     9061 tcatcgtcat cgcaatgctt acaatcaaaa tgccaccagc tactctaaaa gagctaagtg
     9121 aaatgctaaa cgcattgaga atcagattac caaaatagag gctgactaat aaaattactc
     9181 caatagaaac agcggtgatt aagctggtgc gatttctttc cccctcatat tggtgagcgg
     9241 tcatactgaa aaaaataggc aaggaaccaa aaggattgac tatagcaaat agaccaatga
     9301 agaattggag gtagatggca aaattaataa tgatatccac aaaaactttc tcaaacaaca
     9361 gtgtttcggg cgctattata tcggttaatc aaggtagata caaaccaaaa gcacccagcc
     9421 taaaggacgg ttgggtgctt tttgataaaa tgaaaggctt gttagtgtga cgaacgttgc
     9481 tgcataaaat cttcatttac ctcaattgaa gcttttgcac gtagcgattg taagaattca
     9541 ttacgtaaaa ttaaacgatt agcttgttca aattgtggcg ccaatgtctt aaactgttca
     9601 gcattccctt ccgtaatgtt atctaaggca acaataacta catcgccctt ttgattacgt
     9661 gccatttgat aagtcgtttt atcttgcgcc tttttcattg cgaaaatcgt tccagccaat
     9721 accgggtttt ctacttctgc ttgcatataa actaaagttt gtggcgcatt aaatttcacg
     9781 tccactgatt tcccttcatt caaggctttt acattttctt ctactttcgc cagcaacgct
     9841 ttttccgctt tttctgcctt cacaaaattt tcaactgctg cttttgcttc atcaaaagtt
     9901 tgtggacgtt ctgcatcata atcactcact ctcacaaaca atgtctgtgg atttgcatca
     9961 ttcccaacat ctaaagcatc agaattttgt ttattttggc gtagctctcc ttcaaacaaa
    10021 acttttaata ctttctcatt ttgtaaagca gtaggtacgt tttcacgggt aaacagatcc
    10081 gttttttgca gttccacatt accggctttc gcaacttctt caagtgaaga attattctca
    10141 aaggctttat ttgccatttc gcgcgcaact gtaaaatact cagttaacac taattcttgg
    10201 cgaatagttt gggcaatttt atctttcact tgtgctaact caatcacttg ctcaggttta
    10261 cgctccaata ctttgatgat atggtaagca ttatccactt tcaccgctgc actcatctgg
    10321 ccggcagtta aatttgctac agcatcttca aaagctttcg ggaaagtgcc agcttttgcc
    10381 caacctagat caccaccatt atttgctgat aaagtatcgc tagatttgtt ttttgccaaa
    10441 gtagcaaaat cttccccatt tttgagcgct tgctcaatag cctgtgcttc cgcttcacta
    10501 gcgacttgaa tatgagctaa atgggcttca cctttagtta cataatcggc tttattgcga
    10561 tcatagtaag tttgaatttg ctcattggta atctcaactt tgctttccac atcttttgga
    10621 gaaagagtaa cgtactcaac cgctaatttt tcaggtgcta ataatttagt tttatttgct
    10681 tcgtaatagg ctcgcaattc ttgctcggaa gcggtctgat tttccatttc ttttgcaact
    10741 gaaaattcgg ctaaacgcac ttcacgttgt tgcaacagta attttgctaa caatgcctgc
    10801 tgtgccggta cattaaaatc actattgata atcccctctt gtagctggga ttgcaacata
    10861 ccttcctgca caatcgctgc atagccatcg gcacttaaat tattgtggcg taatgtttgt
    10921 tggtataact cgttactgaa tttaccatct tgttgaaaca tctggctgtt tacaatttca
    10981 gatttgattt gatcagcact aatacctagt tttaactctt ttgcatactg gcgcagaagc
    11041 tcttcatcaa ttaatccatt taataccgat tgattaaatt gagcagcata ctccgggcta
    11101 tccattaaat cccaaaagcg actccccagt tcagcattta atctattttg ctcacgatta
    11161 agcgtgttat taaataattg ctgactaatt tcttccccat ttactttcgc tacagaatta
    11221 tcttgtgaaa tcaatcctgt cccaattcca ccgattacaa atgaaataga gactaatgca
    11281 aaaataattt taaagaccgg cccatttgtc cgctcgtgca ttttttcaat catttaaaaa
    11341 ttcctaaatt actcaaaaat tgtcgctgat tatactcttt ctcaaatgag aaataaactt
    11401 gcaaattttt attgaaattt aaccgctttc tctttaaaat tcacagtccc tgtcgtagct
    11461 gagttagaga tcggctattg atgtattatc aacccttaac aaaaggaaat taaaatgtct
    11521 ctaagcgtag aagcaaaagc aaaaatcgtt gctgaatttg gtcgtgatgc aaaagacact
    11581 ggttcttctg aagttcaaat tgcattatta actgcacaaa tcaaccactt acaagcacac
    11641 tttgctgagc ataaaaaaga tcaccacggt cgtcgtggtt tattacgtat ggtttcacgc
    11701 cgtcgtaaat tattagacta cttaaaacgt actgatcttg ctaaatattc ggaaactatc
    11761 gcacgtttag gtttacgtcg ctaatttcaa atattagata tgagagccga gctgaaaagt
    11821 tcggcttttt tataaatttt attcgaggaa aaaatatggc ttttgcgtca ttatttacat
    11881 tattagacga tattgctgcc gtgcttgatg atgtatcgat catgactaaa gtggctgcta
    11941 agaaaacagc aggcgtaatc ggtgatgatc ttgccttaaa tgctaatcaa gtcagtggaa
    12001 agaatgtggt gccagaacga gaactaccga ttgtttgggc tgtagcgaaa ggctcattaa
    12061 tcaataaagt gatcttgatt ccactagcgt tgctgctatc ggtcttttta ccgcaggcta
    12121 ttatcccgct attaatgata ggcggtgcat atttatgttt tgaaggcgta gagaaattac
    12181 tccatcgtct attacataaa aacaatcaag ctcattctga tgaaaccgaa accctttctg
    12241 aaaaagataa aattaaaggt gcaattcgca ccgattttat tctatcagca gagattatca
    12301 ttattgcgtt aggtgttttg caagactctt caaccctaac ccaaatttta tctctctccg
    12361 ctatcggggt cggaattacc ctgtttgttt atggcttagt tggattaatt gtaaaactag
    12421 atgatatcgg tttatggtta atgcagaaaa aaagcacatt ctcacaagcg gttggaaaag
    12481 gcttattatt tgcaatgccg tggtttatga aaggactttc tgtgattggt actattgcga
    12541 tgttcttagt cggtggtggt atttttaccc ataatttacc aatgattcat cacttcgtag
    12601 aacaattcgg tatctctccc catttttcta ctctggttga ttttgcagtg ggtattgtgg
    12661 ttggtattat tgcatgctta attattttac ctttgatgaa attattttct aaaaaggcgc
    12721 actaatcccc aaaagcgtag ctttggttaa agctagcttt ttatctcttg ctataaaaaa
    12781 atagcaggtt tcaagcctgc tattttttta attattataa tgagttagac tcagtggctg
    12841 tttgcggtaa ggctaatggc tcattaactt tagactgagt agccatattc acctgtttga
    12901 gattttcctc acgaatacgg tttgccatta atgtatcccc catctgctct gccacgtgca
    12961 gtgccataat acgatcatca gcaacacatt catcatgagc caataacaat tcaaactgag
    13021 acttcgcttt ttggtattgc ccatctcgtg tataaatata gccaagcgct ctagcataat
    13081 catcggtata tttaacattc gctttatctg cccgttttgc taatactttt agtagcttac
    13141 tgtcagaatc aatttgtaaa cgggttaatt gcacaaataa accttgtaat tgctcatctt
    13201 caaatttctt caacgtttct aaagcaattt cttgtgcaga ttgatgatcg tcactatcaa
    13261 ttaaacgctt gatgacaccg acacgactat acaccgattt acgacgacga tttggttggt
    13321 tttcccacca agaaagcaag ccttcctgcc cttcttcctg taaacgctca tcttgtaaac
    13381 catcatcaac gaaatactct aactcagcat actcttctgc agttaagaaa ttacgttggc
    13441 cgatttcaac taataatcta tctaacgcaa cataagctct ggattcttta taaatttgaa
    13501 tcgctaagcg taatacctca tcgctatgag gtgcaagctc tagtaggcta tcaatcgcag
    13561 ttcgggcagc cggtaatttt ccttgttgta ataaaattcg ggttcgtacc aactccactg
    13621 caacattatt cggtccagct atttcagttg ccttaattaa atatttattt gcagctaaat
    13681 catcaccatt ttgctgagca gcttccgccg ctttaattaa attcagtaca ggctcatctg
    13741 catgttttgc atttttacta aagagttttt ccgcttttga gtaattccct tcactcatac
    13801 gcattaaacc ttccagtgtt tcttgctgag cttttttatg cttacgagtt gagaaccagt
    13861 tataagctcc attactcata cggcatattt tagtgacaat ccattcaagt aaataaacta
    13921 ccgccattgc caacacaaaa aaaaccacaa gcattacaat gctcatttca atcacggtag
    13981 ttgctgtttc aattctgaca taaccttgat ttccggctaa ataaggccca gagattagtc
    14041 caactaataa taaaagcatc aaaaagagcg ttctaaacat aatctattct cctttttatt
    14101 attgtgctga aagaggcatc gcatcttgct ctgcttttac aggctctgct tgctctaatt
    14161 ctttttcagc ttttatttcg actttctcta ttttttcagg ggttttatta agttgctgag
    14221 caatcacctt taagctttgt aatgaagttg gagcatccac ataaatagtt tgttctgcca
    14281 actcatctag ctctttcaag aaatgttgca cattttcatt tgaagtttca aaataacttc
    14341 ttacccaaga gcctaccgtt tgtaacgatt ttttataaag ttcgctttgc tggcgaggaa
    14401 cagctaaaat cgcaatttgt aaacgcaagc gaatgttttc acgcaaataa atttcttgat
    14461 tcggtgcaac aaaggctttc tcatctgctg cattacgttt cgatacacga ataaaatgat
    14521 ctaaaaatga agcagcactt ttttctaaat ttttctccca atccgcaaga gaatcactta
    14581 cttgcccatc ttgtttgcta tttgaagctt gttcactttc caaaatcggc aaatcatcta
    14641 atttattcgc taaatttgcg acacgctgca ttaaggcatt ttgatcgatc tgatgcaatg
    14701 cggttaatgt atttaaatca gttttgattg cctcacgaat gtgaatcgta ttcggtgctt
    14761 ttgataaaac ttgatctgcc tctaacaata aatttttcgc tgtttcaatg tcattatcta
    14821 acaccacttt acgagccgca ttattcaata ggaaatctgc ctcagataat agccagaatg
    14881 tgttatccga accagaggta ttatttagct tttgcagttg aagctgcaaa ctactaattt
    14941 gctgggtata accattttgg gctgtttcta aagctttaat ccgttctaaa gctttttgat
    15001 attccgtgac caattcggca atttgtgctt tttcattttc gaaatttggc atctcaacca
    15061 tagtttgagt agtaggctga tttgctttag cagatactgc ctgaatttga gattcaacct
    15121 ctgcaaactt tttatttgcc atataatgac cagctccgcc aaccgctaaa gccactaata
    15181 gtgcaactaa tgccaagccc tttccgcccg atttttgaac aaccacttgt tcttttggcg
    15241 ctttttgcat ttctgtttga atatctttct ctatagcttc gttcactaca tcttcctcaa
    15301 tctctttttt tgcaaaagat cccgctgaat cgaccgcttg ttgtgcctct tctaccacct
    15361 tttcagctga atgctctaaa tgttcagtta cttttttaga tcttgacata ctcgccacct
    15421 ctagttagat ttgcccactt taaataataa tgtattcatc aaagttacat tatcggcttt
    15481 atccgatacc ctaatcttat cttcttgcca gcccatttgt ttagctatct ttgcaatccg
    15541 ccggctcact acaaccagat cacattcaaa taaccatgct ctacaatctg ctttcgcttg
    15601 ttcataaagt gatcttaaaa tttcactact ggtgatcaca atagtatcta ctcccaagcg
    15661 tttacataag ctgatttttt cgggaatatc atcggtaacc ggctctctac gataacactc
    15721 caaatattga atagttgccc cacgcaagac tgcagtttca gcaagtagct cacgcccaga
    15781 atcggctctt agaattaaaa tagttttatc agttagattc tgcatttcag gtaatgccaa
    15841 aaccccctcg ctattttcaa attctatcgg ataaatgacc gcttgttcag ctttttctgt
    15901 taaatatttc gcagtccgtt gccctaccgc aaaatatttc aaatctgaac gaaaatggaa
    15961 cccggtatct actaatgttt ttgcagcata gtccactgca ttcggcgaaa caacaaatac
    16021 ataatcgtct gattttagcc gagaaatagc cgaaggaagc aaaggtaatt ctttaccggc
    16081 ttcaatcgta aatagcggtt gatgaatagc aaaaatttgc tgttcattca acatatctac
    16141 caattcttgc ccccgactat ccaggcgtgt gaccaacaca ttcattattg tacgttttcg
    16201 ttataaaccg cagctaaaat tttatctgca ccttgagcaa gcaactgttc cgccaccaaa
    16261 atgcctaatt gctccgcctc ttgcttatgt ccttttgccg aagcccgaat aatttgtgag
    16321 ccatctaacg atccaactaa cgcattaagc gttaattcat cgccctctaa tgtagcataa
    16381 ccaccaatcg gcacttggca accgccttgc aaacgggtat tcattgcacg ttctgccatc
    16441 acgcaacaag cggtcgaact atggtttaat tttgcaatgt agttcaaaat ccgttcatca
    16501 ttggctcgag tctcaatccc tactgctcct tgaccagctg cgggtaatga ttgttccaca
    16561 gaaatatagc tacggattcg ctcttgcaag cctaaacgaa ttaagccggc agaggctaaa
    16621 ataatcgcat catactcgcc attatctaat ttgcttaagc gagtgccgac attgcctcgc
    16681 aatgatttaa cctctaaatg cggatatttc gccattaatt ggcactgacg gcgtaagctc
    16741 gatgtaccga ccacagcacc tttcggcaaa tcttctaacg aatggtagtt gttcgacaca
    16801 aacgcatcac ggggatcttc acgttcacaa atcaccgcaa gccccaagcc ttcgggaaag
    16861 ctcatcggca catctttcat tgaatgcacc gcaatatcag ctcgattttc taaaagggct
    16921 aattctaact ctttcacaaa caagcctttt ccgccaattt tggctaatgg ggtgtctaaa
    16981 ataatatcgc ctttcgttac catcgtaacc agttcgacag ataattgcgg aaaatgtttt
    17041 tctaattcgc tttttacaaa atttgcctgc cataaggcta acg
//
LOCUS       DANLYI010000034        16739 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.034, whole genome shotgun sequence.
ACCESSION   DANLYI010000034 DANLYI010000000
VERSION     DANLYI010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 16739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16739
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.034"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            <1..425
                     /gene="nudB"
                     /locus_tag="PXD81_000818"
     CDS             <1..425
                     /gene="nudB"
                     /locus_tag="PXD81_000818"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HDL5536867.1"
                     /translation="HSILVVIYAKNTQRVLMLQRKDDPDFWQSVTGSINIGETPLEAA
                     EREVSEETGLQTFEQKQPLVDCQKQIEFEIFPHFRYKYAPNITHCVEHWFLLPLDSEQ
                     EPILTEHLAYRWVSVEDAVRLTKSPNNAQAIQEYLTSK"
     gene            438..1178
                     /locus_tag="PXD81_000819"
     CDS             438..1178
                     /locus_tag="PXD81_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL5536868.1"
                     /translation="MAGHSKWANIKHRKAAQDAQRGKIFTKLIRELVTAAKLGGGDTS
                     SNPRLRAAVDKALSSNMTRDTINRAIERGVGGGDDTNMETKIYEGYGPGGTAVMVECL
                     SDNANRTISQVRPSFTKCGGNLGTEGSVGYLFSKKGLILVANADEDALTEAAIEAGAD
                     DIQPQEDGSFEIYTAWEDLGSVKDGIEAAGFKIENAEVTMIPSTTVELDAETAPKLLD
                     LINRLEDCDDVQNVYHNGEISDEVAALL"
     gene            1308..1835
                     /locus_tag="PXD81_000820"
     CDS             1308..1835
                     /locus_tag="PXD81_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5536869.1"
                     /translation="MKLVKFVPALAATLVLAACSNANTQSAPQQAQDAVTKVAEQATQ
                     QVNEIAGNAPRASGAVKVEKATDGAKSIVYRCQSGKRVTANYVFQGEEAKAVNLLVQS
                     GGKTQKIPTLTRDASNQDSTSFKTDKYSWDLDTGFTLSSNGQSGMLTKYGAKTDTIVA
                     KLCDVDKALTSKLNK"
     gene            1902..2480
                     /gene="ruvC"
                     /locus_tag="PXD81_000821"
     CDS             1902..2480
                     /gene="ruvC"
                     /locus_tag="PXD81_000821"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HDL5536870.1"
                     /translation="MAIILGIDPGSRVTGYGVIRQNGRQLEYLGSGAIRTTAEDLPTR
                     LKRIYAGVSEIITQFQPNMFAIEQVFMAKNPDSALKLGQARGTAIVAAVNHDLPVFEY
                     AARLVKQTVTGIGSADKVQVQDMVTRMLKLSAKPQADAADALAIAITHAHSIQHSLIV
                     AKQSQQNVSSEKEQILALMKTRYSRGRFRLKG"
     gene            complement(2546..3826)
                     /gene="murA"
                     /locus_tag="PXD81_000822"
     CDS             complement(2546..3826)
                     /gene="murA"
                     /locus_tag="PXD81_000822"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HDL5536871.1"
                     /translation="MEKFRVYGPFSLNGTVDISGAKNAALPILFAAILAEKTVTLKNV
                     PDLKDVDTTFKILRQLGVTVEKGSDKGVVHIDASKINNYVAPYELVKTMRASIWALAP
                     LVARFQLGQVSLPGGCTIGARPVDMHISGLEKMGAKIELDEGYVKATSNGRLNGARIY
                     MDKVSVGATLSVMMAGTLAKGTTVIENAAREPEIVDTADFLNAMGAKISGAGTDMITI
                     EGVEHLGGCEHSVVPDRIETGTFLVAAAISGGRITCRNTKADTLDAVIEKLREAGMQV
                     DVTENTITLDSLGRRPKAVNIRTMPHPGFPTDMQAQFTLLNTVAEGTSRITETIFENR
                     FMHIPELIRMGAKAEIEGNIAICYGVEQLKSAEVMATDLRASISLVLAGCIATGETIV
                     DRIYHIDRGYEHIEEKLRQIGARIERFSAPFEGE"
     gene            complement(3936..4196)
                     /locus_tag="PXD81_000823"
     CDS             complement(3936..4196)
                     /locus_tag="PXD81_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005598336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA/IbaG family iron-sulfur metabolism protein"
                     /protein_id="HDL5536872.1"
                     /translation="MNPSQIEEILKQALSDATEVHAQGENAHFGVIVVSDSIASLSKV
                     KQQQAVYAPLAEYFTTNAIHALTIKVFSVEKWKTERLLNMVG"
     gene            complement(4229..4552)
                     /locus_tag="PXD81_000824"
     CDS             complement(4229..4552)
                     /locus_tag="PXD81_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HDL5536873.1"
                     /translation="MPQKTLQWDIQQNNESLFVKLSGELTRNTLLPLWKQRASFLSPT
                     GKQSLYWDLKEITRIDSAGFTLLAELLNHYHKIIPNSLINTPDSVRTLAALYDLDGWF
                     EQFII"
     gene            complement(4564..5199)
                     /gene="mlaC"
                     /locus_tag="PXD81_000825"
     CDS             complement(4564..5199)
                     /gene="mlaC"
                     /locus_tag="PXD81_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HDL5536874.1"
                     /translation="MLKNIKKIIVTGLVAVSALFSTTAFAETSPYVLMQQTADKLFGD
                     IKANQAKIKSNPEYLRTIVRNDLMPHVHVKYAGQLVLGKNLSAATDAQKEAFFNAFGQ
                     FVEQSYAQVLTQYQDQSVQIESPKSVDGKSIVSIRVNVSGKGAAQPIKLDFKWRKNSK
                     TGEWQAYDMAAEGVSMVATKQNEWAGVIRQKGIDALIAQVAQSAKQPITLK"
     gene            complement(5245..5769)
                     /gene="mlaD"
                     /locus_tag="PXD81_000826"
     CDS             complement(5245..5769)
                     /gene="mlaD"
                     /locus_tag="PXD81_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HDL5536875.1"
                     /translation="MRQSIKYEFWVGLFVLLGLAALVFLGLRVANVQGFSNEKTYTLY
                     ATFDNIGGLKVRAPIKVGGVVVGRVADISLDNKTYTPKVALAVNESFNQIPDTSSLSI
                     KTSGLLGEQYVALNVGFVIEGETAMMKEGDTFVDTNSAMVLEDLIGQFLYGDKKSDKT
                     EDEKASDSAEPKAE"
     gene            complement(5790..6566)
                     /gene="mlaE"
                     /locus_tag="PXD81_000827"
     CDS             complement(5790..6566)
                     /gene="mlaE"
                     /locus_tag="PXD81_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HDL5536876.1"
                     /translation="MVNFISSIGAVVINFIRTLGRSAFMLWGALIGKPEFRKHTPLLI
                     KQLYVLGVQSLLIIMLSGLFIGMVLGLQGYVVLVDFAAESSLGTLVSLSLLRELGPVV
                     TALLFAGRAGSALTAEIGLMKATEQLSSLEMMAVDPLRRIIAPRFWAGVISMPVLAAI
                     FTAIGIWGGSLVGVDWKGVDGGSFWSVMQNSVNTSDLINGFIKSVIFAFAVVWIALFN
                     GYDCVPTSEGISQATTRTVVNASLVILGLDFILTAIMFGG"
     gene            complement(6559..7362)
                     /gene="mlaF"
                     /locus_tag="PXD81_000828"
     CDS             complement(6559..7362)
                     /gene="mlaF"
                     /locus_tag="PXD81_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HDL5536877.1"
                     /translation="MTKTENLVEVKNLTFKRGERVIYDNLNLQVQKGKVTAIMGPSGI
                     GKTTLLRLIGGQILPESGEILFDGRDICKANNKELYEIRQRMGMLFQSGALFTDISTF
                     DNVAFPIREHTRLPEELIRKLVLLKLEAVGLRGAANLMPSELSGGMARRAALARAIAL
                     DPDLIMYDEPFTGQDPISMGVIVELIKELNQALNLTSIVVSHDVKEVLSIADYAYIVA
                     DKRVIAEGTPEILQASEDPQVVQFLAGKADGPVRFHYPAKDYTEELFNG"
     gene            7587..8981
                     /locus_tag="PXD81_000829"
     CDS             7587..8981
                     /locus_tag="PXD81_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005617770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HDL5536878.1"
                     /translation="MNKTMKKSFLTAFILGSTIAAMPAYAEATLPTAVNGQAVPSLAP
                     MLEKVRPAVVSIAVEGKTKGDSRRSREIPEEFEFFFGPHADLFGNRSSAPRNFRGVGS
                     GAVINAEKGYVITNNHVIDNADKITVKLEDGREFKAKLVGADPLSDVALIQVEDPKNL
                     TEIKFADSDKLQVGDFTVAIGNPFGLGQTVTSGIISALGRSTGQSDEGYENYIQTDAA
                     VNQGNSGGPLINLNGELIGINTAIISPSGGNAGIAFAIPSNMANSLVTQIIEFGEVKR
                     GMLGIKGGELNADLAKEFNISTQQGAFVSEVLPDSAAAKAGFKAGDVITEINGQKIRS
                     FSELRAKVATSGVGKEIELTYLRDGKEAKAKVTLQSDSEAKVTASNLIPALKGADFNN
                     YNAKGIKGVEISNVEKGSIAEMRSLKKGDIIIGVNRHSVENIADLRKILETKPSVVAL
                     NIVRDGANFYVILQ"
     gene            complement(9033..9794)
                     /locus_tag="PXD81_000830"
     CDS             complement(9033..9794)
                     /locus_tag="PXD81_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C biogenesis protein"
                     /protein_id="HDL5536879.1"
                     /translation="MKTREQLSQENYQQAVKFAQHFADDVRQEFEREAEQRYQFERTQ
                     FEQSVLQNNAQNRPLAKIASLSCVAILLLAMTYYGLSGRYQVVQSGLQQHQDFQQQMS
                     DAHGTSKNERYILSLQNQLRENPNNGDLWYELGQAYALSNDFDSALVCYDNAEKLLGK
                     RAAILGAIATARYYDNGQKMTVEIQAIIEQALQLDKNENASLLLLASDSFLNNNFQQA
                     LDNWRKVLDSNNDSINRRAIIQSMEMARQMLNSQQ"
     gene            complement(9781..10233)
                     /gene="nrfF"
                     /locus_tag="PXD81_000831"
     CDS             complement(9781..10233)
                     /gene="nrfF"
                     /locus_tag="PXD81_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HDL5536880.1"
                     /translation="MRNSIISIGFLQKIRKILPLVFGLLLFVGTVQAEMVDTFQFQNE
                     ADRVRAISLARSLRCLQCQNQNLVESNATAAYNMRIEVYEMVNQGKSNEEIIRIMTER
                     FGSFVNYDPPFTPQTWVLWGLPVGLFSLLFLAILWRTRKNKGNKNENA"
     gene            complement(10223..10756)
                     /locus_tag="PXD81_000832"
     CDS             complement(10223..10756)
                     /locus_tag="PXD81_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005604798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HDL5536881.1"
                     /translation="MKKILLLIPLLLLVVIVGFLTVPLMNKDALAPTEDWQGKPFPEF
                     VGKNLLDSNAHLNNNSLPKEPYILNVWVSWCTWCIKEFPILLELKQKGVPIVGLTYSD
                     RPNDALNALKNWGNPFSLIIDDYEKGFLIQTLKVSSAPSSYLIDKHGVVRYQQKGYNP
                     DFAQDFLPKLEALRNEK"
     gene            complement(10753..12657)
                     /gene="nrfE"
                     /locus_tag="PXD81_000833"
     CDS             complement(10753..12657)
                     /gene="nrfE"
                     /locus_tag="PXD81_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfE"
                     /protein_id="HDL5536882.1"
                     /translation="MLPELGYFSLLLAAISAIFQVGFALLGEVRKQYHWLALAPLFTY
                     LQAAFTTFSFSTLAYAFLTDDFSVIYVAAHSNSQLPDFFKFAATWGGHEGSMLFWLTA
                     LVIWSAVFCKFSQKIDRLFANRTLAMLGLIALGFLLFILLVSSPFERGFPPPPEGRDL
                     NPMLQDIGLIFHPPLLYLGYVGFAVSFAMIVAALVSGGMDAAIMRWLRPWTMISWGFL
                     TAGIILGAWWAYYELGWGGWWFWDPVENASLMPWLLGTALVHSLIVSQQRGIFYYWTI
                     LLAIFAFALSLLGTFIVRSGVLTSVHAFAVDPDRGMALLILFFGLSFIALALFAFRAN
                     LWQGKVRFNLLSKETAFLLINGLFSVAASVVLLGTFYPMIFTVMNWGSISVGAPYFNS
                     VFTPLALLLMIVMGFAVVLGWKQISNKQILVKLWLLPIAVGLALGLIYASISQREHFA
                     LQPLPTIFISLAIWIILTHLPYSQKMWQIRPLAMRLAHIGFAVCVIGAMMNSYYGDEI
                     GVRLKPNQAAELAGYTFKYEDYHDLIGPNYTAEQAVFSISRDEQLLATVTPERRYYDV
                     RTMTMAEVGLFHHNLDDIYIVMGDKFGNLEYAFRLHYKPYVQALWLGGMMMVMAALLA
                     LFGYRRENKK"
     gene            complement(12927..13874)
                     /gene="nrfD"
                     /locus_tag="PXD81_000834"
     CDS             complement(12927..13874)
                     /gene="nrfD"
                     /locus_tag="PXD81_000834"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011848317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HDL5536883.1"
                     /translation="MNEYVPFQTPNLVWDGTIAIYLFLLGISSGAVQLAIAYRNSGVK
                     MEKPSQNWIIRSAAILGTLPTIIGLLLLIFHLTKPWTFWKLMFNYNFTSVMSMGVMLF
                     QLYMAVLVVWIAIMFKDWIVRYLPVVSGLIDFAEAKLLKPIEFILFVLAAVLGAYTGF
                     LLSALVSYPMLNNPVLPALFLASGASSGIAATFLVILLAGKLSGESHEVHFMHKFEVP
                     IMVTELGLIVAFFVGLHFGGADKSLALTHALNGFWGNVFWIGVMLIGIAIPLVANIFG
                     SHRLKHNVKFIILISVFDLIGVLCLRYFILYAGQLTVAS"
     gene            complement(13874..14551)
                     /gene="nrfC"
                     /locus_tag="PXD81_000835"
     CDS             complement(13874..14551)
                     /gene="nrfC"
                     /locus_tag="PXD81_000835"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007524349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HDL5536884.1"
                     /translation="MTCTRRDFVSGAGAIAVVASTGLATSITLATEKEQKKIRYAMVH
                     DETACIGCTACMDACRTTNKVPEGVSRLEIIRSEPFGEFPNVQYEFFRQSCQHCTNAP
                     CVSVCPTGASFIDPETGIIDVHSDLCVGCQYCIAVCPYRVRFIHPEKKSADKCNFCRD
                     TNLAEGKQPACVEACPTKALTFGDMNDPSSDIFHKVKNNPVYRTKTALGTEPNLYHIP
                     FGKGEHR"
     gene            complement(14551..15207)
                     /gene="nrfB"
                     /locus_tag="PXD81_000836"
     CDS             complement(14551..15207)
                     /gene="nrfB"
                     /locus_tag="PXD81_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HDL5536885.1"
                     /translation="MKKLISKAGRAVALIQAVVFAGFFAISSANALMPEQPKPLWQSE
                     TADDAKVAQDARRDPNKYCTQCHESANATGKPFHHAGKHFQKDVKSPNNGEPLTCISC
                     HGNISESHRKGAKDVMRFNPHGKASNPSLERSVNEQNQVCFACHTAEKLREKFWAHDT
                     HATKIACTNCHEIHPEKDPMKDIPEKDRIKLCTDCHTKIHSGEFKNPEMKATEAKGTK
                     "
     gene            complement(15226..16728)
                     /gene="nrfA"
                     /locus_tag="PXD81_000837"
     CDS             complement(15226..16728)
                     /gene="nrfA"
                     /locus_tag="PXD81_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HDL5536886.1"
                     /translation="MKFLIKSLAVATISILGCLQTALAEEAKTESLTDKAVKHEKLGV
                     KIESANHLFAEKYPLQYDSWKSTAKSTDRGSALEADPRYVILWAGYAFAKDYNKPRGH
                     FYAVTDVRDILRTGAPKDENDGPQPMACWTCKGPDVPRLIEEKGERGYFDPKWAKYGA
                     EIVNSIGCADCHDTTSEEFKQGKPALRVARPHVLRALNTVGWKFEDLDKHGKRPAVCA
                     NCHVEYYFKNKTDVTFPWDKGVDVDSIEKYYDEINFTDWTHALSKAPMLKTQHPDFEV
                     WSQGTHGKNGVTCIDCHMPKVKDKDGKVYTDHKIGNPFDQFEATCKTCHEQSKETLEK
                     RVKEYKHEVKEAMIRLEDQLVKAHFEAKAAWEAGATQEEMKEILMAIRHAQWRWDYSA
                     AGHGNHFHAPDVMLRTIATGIDRAADARAKLGVVLAKHGVTTPVAIPDISTKEKAQLA
                     IGLDIPKEQAAKDEFLKTVVPQWEKEARAKGLLPAEEADKPVAAPKAEAK"
BASE COUNT         4991 a         3721 c         3327 g         4700 t
ORIGIN      
        1 ctcatagtat ccttgtggtg atttatgcta aaaataccca acgagtattg atgctacaac
       61 gcaaggacga tcctgatttt tggcaatcgg tgaccggttc gatcaatatt ggagaaacac
      121 cgctggaagc agcagaacgg gaagtgagcg aagaaaccgg tttgcaaact tttgagcaaa
      181 aacaaccgct tgttgattgc caaaagcaga tagaatttga aattttcccg cattttcggt
      241 ataaatacgc acctaatatc acacattgtg tcgaacattg gtttttgctg ccacttgata
      301 gtgagcaaga gcctatttta accgaacatt tggcatatcg ttgggtttca gtggaagatg
      361 cggttcgact gactaaatct cccaacaatg cccaagcaat acaagaatat ttaacatcaa
      421 aataaggaaa caacagaatg gcaggccata gtaagtgggc aaatattaaa caccgtaaag
      481 cggcacaaga tgcacaacgc ggtaaaattt ttactaaatt aattcgtgaa ttggtgactg
      541 ccgccaaact cggcggtggt gatacctctt ccaaccctcg tttgcgtgcg gcggtggata
      601 aagcgttatc cagcaatatg acccgtgata ccatcaaccg tgcaatcgag cgtggtgtag
      661 gcggtggcga tgataccaat atggaaacca aaatctacga aggttacggc ccgggtggta
      721 cagcagtgat ggtggaatgt ttaagtgata acgctaaccg tacgatttcc caagttcgtc
      781 caagtttcac aaaatgtggc ggtaacctag gtactgaggg ttcggtaggt tatttattta
      841 gcaaaaaagg cttaatttta gtggcaaatg ccgatgaaga tgccttaact gaagctgcta
      901 ttgaggccgg tgcggacgat attcaaccac aagaagatgg tagctttgaa atttataccg
      961 cttgggaaga tttaggctca gtgaaagacg gcattgaggc tgcaggcttt aaaattgaga
     1021 atgcagaagt aacaatgatt ccatcaacta ccgttgaact tgacgcagaa actgcaccaa
     1081 aacttcttga tttaatcaac cgccttgaag attgtgatga tgtacaaaac gtttatcaca
     1141 atggtgaaat cagtgacgaa gtcgctgcat tactgtaata aaaaaatcac gcaaaataag
     1201 cataatttag gttatgctta ttttttatga acttttttcc tatattactg actaacctaa
     1261 tgtagtttat atgctactac tttactataa cattttaagg agagtctatg aaattagtga
     1321 aatttgtccc tgccctagca gcaactttag tattagccgc ttgctctaat gcaaacacac
     1381 aatctgctcc tcagcaagct caggacgctg tgactaaagt ggctgaacaa gcaacacagc
     1441 aagtgaatga aattgcaggt aatgcacctc gtgcaagtgg tgcagtaaaa gtagaaaaag
     1501 caactgatgg tgcaaaatca attgtatatc gttgccaaag tggtaaacgt gtaactgcga
     1561 attatgtctt ccaaggagaa gaggcaaaag cagtaaatct attagtccaa agcggcggta
     1621 aaacacaaaa gatcccaaca ttaactcgtg atgcctcaaa ccaagattct acttcattca
     1681 aaacggataa atattcttgg gatttagaca caggctttac cttatcatct aatggccagt
     1741 caggtatgct aaccaagtat ggtgctaaaa cagataccat cgttgctaaa ttatgtgatg
     1801 tagataaagc attaacctca aaattaaata aataatcttt cttgatttta ctactcataa
     1861 gcgagaagat aatatcttct cgcttatttt attgcacaaa gatggctatt attttaggaa
     1921 ttgaccccgg ctcaagggta acaggttacg gtgtgattcg ccaaaacggg cgacagctgg
     1981 aatatttagg aagtggggct attcgaacca cggctgaaga tctgcctacc cgcttaaaac
     2041 ggatttatgc aggcgtgagt gaaatcatca ctcaatttca accaaatatg ttcgccatag
     2101 agcaggtatt tatggcaaaa aaccctgatt ctgccttaaa gctcgggcag gctcgaggaa
     2161 cagcgattgt agcagcggtt aatcacgatt tacctgtgtt tgaatatgcc gcccgattag
     2221 tcaaacaaac cgttacggga attggttctg ctgataaagt acaagtgcaa gatatggtaa
     2281 cccgtatgct taaactctcc gccaaaccac aagccgatgc ggctgatgca ttagccattg
     2341 cgattaccca cgctcattca atccaacatt cactgattgt tgctaagcaa agccagcaga
     2401 atgtgagtag tgagaaagag caaattttag cactaatgaa aacccgttat agccggggaa
     2461 ggtttaggtt aaaaggctaa ctcaatataa aatgaccgct tggaactaat gtttcaagcg
     2521 gtcatttttt gagaaaattt tacaattact cgccttcaaa aggagctgag aaacgttcaa
     2581 ttctcgcccc aatctgacgt aatttttctt cgatgtgttc atagccacgg tctatgtggt
     2641 aaattctatc cacaatcgtt tcacccgttg caatacagcc tgccaacact aagctgatgg
     2701 aagcacgcaa gtcagttgcc attacttctg ctgattttaa ctgctctaca ccatagcaaa
     2761 ttgcaatatt accttcaatt tccgctttgg cacccatacg aatcagctca ggaatatgca
     2821 taaaacggtt ttcaaaaatc gtttctgtaa tacggcttgt gccttctgca actgtattta
     2881 ataaggtaaa ttgagcttgc atatcagttg ggaaacctgg gtgtggcata gtgcgaatgt
     2941 tcactgcttt cgggcgacga cctagagaat caagcgtgat agtattttcc gtcacatcaa
     3001 cctgcattcc agcttcacgt aatttttcga tcactgcatc aagagtgtct gctttggtgt
     3061 tacggcaagt aattcgacca ccggaaatag ccgctgccac taaaaatgta ccggtttcaa
     3121 tacgatccgg cactacgctg tgttcgcaac cgcctaaatg ctcaacccct tcaatggtaa
     3181 tcatatcggt acctgcacca gaaattttag ctcccatcgc atttaagaaa tcggcagtat
     3241 ctacaatttc aggctcacga gcagcatttt caataacagt agtacctttt gctagagtac
     3301 ctgccatcat caccgataaa gttgctccta cgctcacttt atccatatag attcttgcac
     3361 cgtttaaacg accgtttgaa gttgctttga catagccttc atcaagctcg attttcgccc
     3421 ccattttttc aagaccggaa atgtgcatat ctaccggtct tgccccgata gtacaaccgc
     3481 ccggtaaaga aacttgccca agttggaacc gggcaactaa tggagctaac gcccaaattg
     3541 aagcacgcat tgttttcact aattcgtaag gtgcaacgta attattgatc ttactcgcat
     3601 caatatgtac cacgccttta tcgctacctt tttcaaccgt tacccctaat tggcgtaaga
     3661 ttttaaaagt ggtgtccaca tcttttaaat caggcacatt ttttaacgta actgtttttt
     3721 cagccaaaat agcggcaaat aaaataggga gtgcggcatt tttcgcacct gaaatatcga
     3781 cagtaccatt taaagaaaat ggaccataca cacgaaattt ttccatttgg gagatcctaa
     3841 aaaataataa tttgtagggg cgaaaaattt ttcgcccaat tttccaacgt ttacaaaaga
     3901 gtttgggcga atagtgattc gcccctacat ttcatctatc caaccatatt tagcaaacgc
     3961 tcagttttcc atttttccac gctgaatact ttaatggtta aagcgtgaat cgcattagta
     4021 gtgaaatact cagctaaagg ggcataaaca gcttgctgtt gttttacttt tgaaagagaa
     4081 gcaatgctgt cacttactac aataacgcca aagtgggcat tctcgccttg agcgtggact
     4141 tctgttgcat cagataatgc ttgttttaaa atttcttcaa tttgtgatgg attcattctg
     4201 acttcctaaa atatgattgg taactcactc aaataataaa ttgctcaaac caaccgtcta
     4261 aatcgtataa tgcagccaaa gttctgacag aatctggcgt attaatcaaa ctgtttggta
     4321 tgattttatg gtaatgattc agtaattcag caagtaaggt aaaaccggca gaatcaatcc
     4381 gagtgatttc ttttaaatcc caatataggc tttgtttccc tgttggagat aaaaaagaag
     4441 cacgttgctt ccataaaggg agcaacgtgt tacgagttaa ttcgcctgat agtttcacaa
     4501 aaagactttc gttattttgc tgaatgtccc attgtaatgt tttttgaggc ataactaaac
     4561 tacttatttt aaagtgatag gctgttttgc agattgtgcc acttgtgcaa ttaaggcatc
     4621 aatccctttt tgacgaatca cacccgccca ttcgttttgc ttggttgcaa ccatacttac
     4681 tccttcagcg gccatatcgt atgcttgcca ttcacctgtt ttgctgtttt tacgccattt
     4741 aaaatctaat ttgatcggct gtgcagcacc tttacctgac acgtttacac gaatgcttac
     4801 gatagattta ccatccaccg atttcgggct ttcaatttgc acactttgat cttgatactg
     4861 ggttaacacc tgagcataag actgctcaac aaattgacca aaagcattaa agaatgcctc
     4921 tttttgtgca tcagtcgcag ccgataagtt tttccctaac actaattggc ctgcatattt
     4981 cacgtgaacg tgtggcatta aatcgttacg cacaatggta cgcaaatatt cagggtttga
     5041 tttaattttc gcttgatttg ctttaatatc gccaaataat ttatctgcgg tttgttgcat
     5101 taatacataa gggctagttt cagcaaatgc ggtggttgaa aataatgcag aaactgccac
     5161 taagcctgtt acgataattt ttttaatgtt ttttaacatt attgatgctc tccaaaactc
     5221 taaattagag gttgttgtac ttaattactc tgcttttggt tcagcagaat cacttgcttt
     5281 ttcatcttca gttttatctg actttttatc accatataag aactgaccga ttaaatcttc
     5341 taacaccatt gcagaattgg tatctacaaa agtatcacct tctttcatca ttgcagtttc
     5401 accttcgatt acgaagccaa catttaacgc aacgtattgc tcgcctaata agcctgaggt
     5461 tttaattgag agcgagctgg tatcgggaat ttgattaaaa ctttcattta ccgctaatgc
     5521 cactttcggg gtgtaggttt tgttatcaag tgaaatatca gccactcggc caactaccac
     5581 accgccaact ttaataggcg cacgaacctt tagtccacca atattatcaa aagttgcata
     5641 cagggtgtaa gttttctcat ttgagaagcc ctgcacattt gctactctta agcctaaaaa
     5701 gacaagagct gcaagcccaa gtagtacaaa taagcctacc caaaattcat atttaattga
     5761 ttgacgcatt gtctgttcct ttcaaaaaat caaccgccga acattatcgc agttaaaata
     5821 aaatccaatc ctaaaatcac cagtgaagca tttaccaccg ttcttgtggt cgcttggctg
     5881 attccttctg aagtcggtac gcaatcgtag ccgttaaata aggcaatcca gaccaccgca
     5941 aaggcaaaaa tcacactttt aataaagccg ttaattaaat cgctggtatt taccgagttt
     6001 tgcattaccg accaaaaact gccaccatca acgcctttcc aatctacccc gactaatgag
     6061 ccgccccaaa ttccgatagc ggtaaaaata gcagctaaca ccggcattga aatcacgcct
     6121 gcccaaaagc gaggagcgat gatacgtcta agcggatcaa ccgccatcat ttcaagactg
     6181 gaaagttgct ctgtggcttt catcagcccg atttctgcgg tcaatgccga accggctcgc
     6241 cctgcaaata ataatgcagt aactacagga ccaagttctc gtaaaagcga gagtgaaacc
     6301 aaagttccca agctactttc ggcggcaaaa tcgaccaata ccacatagcc ttgtaagcct
     6361 aacaccattc cgataaataa gcccgacagc ataataatta agagcgattg cactcctaac
     6421 acataaagct gtttaatcag taacggggta tgttttctaa attcaggttt accgattaat
     6481 gctccccata acataaaagc ggaacgccct aatgtacgga taaaattaat caccaccgcc
     6541 ccgatagagc taataaaatt aaccattaaa tagctcctcc gtataatctt ttgctggata
     6601 atggaaccgc accggaccat cagctttacc ggctaagaat tgtaccactt gcggatcttc
     6661 gcttgcctgt aaaatttccg gtgtaccttc agcaatcact cgtttatctg ccacaatata
     6721 agcataatcg gcaatactta atacttcttt cacatcgtga gacaccacaa tggaggtcaa
     6781 attcaatgcc tgatttaact ctttaatcag ctcgacaatc acacccatac taatcggatc
     6841 ttgcccggta aacggctcat catacataat cagatccgga tctaacgcaa tcgctcgagc
     6901 aagggcggca cgtcttgcca taccgccgga aagttcggac ggcattaaat ttgccgcacc
     6961 tcgcaaccca accgcctcga gcttcaataa caccaatttg cgaattagct cttcgggtaa
     7021 acgggtatgc tcccgaatcg ggaaagccac attatcgaag gtagaaatat cggtaaataa
     7081 cgcccccgat tgaaacaaca tccccattcg ttggcgaatt tcatacaact ctttgttatt
     7141 ggctttgcaa atatctctgc catcaaataa aatttcgccc gattcaggta aaatctgccc
     7201 accgattagc ctcaacaggg tggttttacc aatcccagaa ggccccataa tggcagtcac
     7261 tttacctttc tgcacttgca gattcaggtt atcgtaaatc actcgctccc ctcgcttgaa
     7321 ggtcaagttt tttacttcaa ccaaattttc cgtttttgtc atcaaatagg actcaaatta
     7381 ataaaagtga tatttatggt ctatcaaatt tgccagaatg tcaaaaaact tttaataatc
     7441 tgacaaaaat ctgcctttac aagcggtcag atttgctaaa ttttttgcaa accggctgaa
     7501 ctgaaaaaga aaggtcttgt cttagccaat acgagcgttt tcactcgtag attataaaaa
     7561 cataggatat gaggaacatt cacaatatga acaaaacaat gaaaaaatca tttttaactg
     7621 cattcatttt aggctcaacc attgccgcaa tgccggcgta tgcagaagca acattaccca
     7681 cagccgtaaa tgggcaagct gtgccaagcc ttgcaccaat gctggaaaaa gttcgcccgg
     7741 cggtggtgag tattgcagta gaaggtaaaa ccaaaggcga tagccgccgc agtcgtgaaa
     7801 ttccggaaga atttgaattt ttcttcggtc ctcacgccga tttattcggc aaccgctcat
     7861 cagctccacg caatttccgt ggcgttggat caggtgcagt catcaatgcc gaaaaaggtt
     7921 atgtgattac caataaccac gtgatcgaca atgccgataa aattaccgta aaacttgaag
     7981 acggacgtga atttaaagct aaattagttg gtgctgatcc cctttcagat gtggcgttaa
     8041 ttcaagttga agatccaaaa aacttaactg aaattaaatt cgccgattcc gataaattac
     8101 aagtgggcga ttttacagtt gcaatcggta acccgtttgg cttaggacaa actgttactt
     8161 caggcatcat ctcggcactt ggtcgttcaa ccggtcaatc cgatgaaggc tatgaaaact
     8221 atattcaaac cgatgcggca gttaaccaag gtaactctgg cggcccgtta attaacttaa
     8281 acggcgaatt aatcggaatt aacaccgcta ttatttctcc aagtggtggc aatgccggta
     8341 tcgcctttgc gattccaagt aatatggcaa acagcttggt gactcaaatt attgagtttg
     8401 gtgaagtaaa acgtggaatg ttaggcatta aaggcggtga attgaatgct gaccttgcta
     8461 aagagttcaa tatttccacc caacaagggg catttgtaag cgaagtctta ccggattctg
     8521 ctgctgctaa agccggcttt aaagcaggtg atgtgattac tgaaatcaac ggtcaaaaaa
     8581 tccgtagttt cagcgaatta cgagcaaaag tagctacttc aggtgtaggt aaagagattg
     8641 aacttactta cctgcgtgac ggcaaagaag ccaaagcaaa agtgacctta caatcggatt
     8701 ctgaagccaa agttactgct tcaaacctca ttccggcatt aaaaggggca gattttaaca
     8761 actacaatgc caaaggcatt aaaggggttg aaatttctaa cgtagaaaaa ggctcgattg
     8821 ctgaaatgcg aagcctcaaa aaaggcgata ttatcatcgg cgtgaaccgc cactcggtag
     8881 aaaacattgc cgatttacgc aaaatattag aaaccaagcc atctgtggtt gcactcaata
     8941 ttgtgcgtga cggtgcaaat ttctatgtga ttttgcaata aaccttttga tccactcaaa
     9001 caaaagccga tgacaaaaat catcggcttt tgttattgct gggaatttaa catctgtcgt
     9061 gccatttcca tactttggat aatcgctctg cggttgatag aatcattatt gctatctaac
     9121 actttacgcc aattatcgag agcttgttga aaattgttat ttaaaaagct gtcggaggct
     9181 agcaggagta aactggcatt ttcattttta tccaactgta gggcttgctc gataatggct
     9241 tgaatttcaa cggtcatttt ctgcccgtta tcgtaataac gagcggtggc aatcgccccc
     9301 aaaatagccg ctcgttttcc gagtagcttc tcggcattat cgtagcaaac cagtgccgaa
     9361 tcaaaatcat tgcttaacgc ataagcctgc cctagttcgt accataaatc accgttattc
     9421 gggttttctc gaagctggtt ttgcagactc aaaatatagc gttcattttt cgatgtgccg
     9481 tgtgcatcgc tcatctgttg ttgaaaatct tgatgttgtt gcaaacctga ttgcaccact
     9541 tgataacgtc cggaaagccc atagtaagtc attgctaaca acaaaatcgc cacgcaactc
     9601 aggcttgcga ttttagcaag cggtcgattt tgagcattat tttgcaaaac gctctgttca
     9661 aattgcgttc gttcaaattg gtaacgttgc tctgcctcac gctcaaactc ttgtctgacg
     9721 tcatctgcaa aatgttgggc aaatttaacc gcttgttggt agttttcttg gcttaattgc
     9781 tcacgcgttt tcatttttgt tgcctttatt tttgcgagtt cgccacaaaa tagctagaaa
     9841 taacaggcta aacaagccaa ccggcaagcc ccataatacc catgtctgtg gcgtgaatgg
     9901 tggatcgtaa ttcacaaaac tgccgaaacg ctcggtcata atgcggataa tttcctcatt
     9961 acttttccct tgattcacca tttcatacac ttcaattcgc atattgtaag ccgccgtcgc
    10021 attggattcg accaaatttt ggttctgaca ttgcaaacat cgcaacgaac gagccagtga
    10081 aatcgcccgt acccgatccg cctcattttg aaattgaaag gtgtccacca tttcagcttg
    10141 aaccgtgccg acaaatagca acaagccaaa tacaagcggt aatattttgc ggattttttg
    10201 caaaaagccg atggaaatta tgctatttct catttctgag agcctctaat ttgggtagga
    10261 aatcctgtgc aaaatcaggg ttgtaaccct tttgttggta acgcactaca ccgtgtttat
    10321 caatcaaata actggaaggt gcagaagaga ctttcagggt ttggatcaaa aagccttttt
    10381 cgtaatcatc aataattaaa ctaaacggat ttccccagtt tttcaatgcg ttgagggcat
    10441 catttgggcg gtcgctataa gttaagccga caatcggcac gcctttttgt tttagttcca
    10501 acaaaatcgg gaactcttta atgcaccaag tacaccagct cacccacaca ttgagaatat
    10561 aaggctcttt cggcaagctg ttgttattta aatgcgcgtt actatcaagt aagttcttgc
    10621 ctacaaactc tggaaacggc ttgccctgcc aatcttcagt gggagcaagg gcatctttat
    10681 tcattaacgg cacagtcaaa aagccaacaa taactacaag taacaggagc ggaataagta
    10741 acaggatttt tttcattttt tgttttctct acgataaccg aataatgcca acagggcggc
    10801 cattaccatc atcataccac ctaaccataa ggcttgcaca taaggcttat agtgcaaacg
    10861 gaaggcatat tctaaattgc cgaacttatc ccccatcaca atataaatat cgtctaaatt
    10921 gtggtggaaa agccctactt ctgccattgt catcgttctt acatcataat agcgtctttc
    10981 cggtgtgacg gttgccagca actgttcatc tcggctgata gaaaaaacgg cttgctcggc
    11041 ggtgtagttc ggaccgatga gatcgtgata atcttcatat ttaaaggtat aaccggcaag
    11101 ttccgctgct tgattcggct ttaaccgcac gccgatttca tcgccgtaat aactgttcat
    11161 cattgcccca atcacgcaaa ccgcaaagcc gatatgggct aaccgcatcg caagcggtcg
    11221 aatttgccac attttttgcg aatagggcaa atgagtgaga ataatccaga tcgcaaggct
    11281 gataaatatg gtgggtaacg gctgcaaagc aaaatgctct ctttgcgaga tggaggcata
    11341 aatcaaccct aacgctaaac cgactgcaat cggcagcaac caaagtttta ccaatatctg
    11401 cttatttgaa atctgtttcc agcccagcac caccgcaaag cccattacta tcattaacag
    11461 taacgctagt ggcgtgaata cgctattgaa atagggggcc cccactgaaa tcgatcccca
    11521 attcatcact gtgaagatca tcgggtagaa agtccccagc aataccacag aagcagccac
    11581 actaaataag ccgttaatca ataaaaatgc cgtttcttta gaaagtaagt tgaaacgcac
    11641 tttgccttgc cagagattcg ctcggaatgc aaacagagca agggcaatga aacttaagcc
    11701 gaagaataag atcagcagtg ccattcctct gtcgggatcg accgcaaagg catgaaccga
    11761 ggtcagtaca cccgaacgaa caataaacgt gcctaatagg cttaaagcaa aggcgaaaat
    11821 ggcaagtaga atcgtccaat aatagaaaat gccacgctgt tggctcacaa ttaaactatg
    11881 caccaatgcg gtgccaagta gccaaggcat tagggaggca ttttccaccg gatcccaaaa
    11941 ccaccagcca ccccagccga gttcgtaata tgcccaccac gcccctaaaa taatgccggc
    12001 ggtgagaaat ccccacgaaa tcatcgtcca cggacgaagc caacgcataa tcgcagcgtc
    12061 cattccgcct gaaacgaggg cggcgacaat cattgcaaag ctcaccgcaa agccgacata
    12121 gcccaagtag agcaaaggcg ggtgaaagat gagcccaata tcttgcaaca ttgggttgag
    12181 atctcgccct tcaggtggtg gtggaaagcc acgttcaaat gggctggaaa ccaatagaat
    12241 gaaaagtaga aaacctagtg caattagccc taacatcgct aaggttcggt tggcaaacaa
    12301 gcggtcaatt ttttgcgaaa atttgcaaaa taccgcagac caaatcacaa gagcggtgag
    12361 ccaaaacagc attgagcctt cgtgtccgcc ccaagtggcg gcaaatttaa aaaaatcggg
    12421 gagctggctg ttggaatgag cggcaacgta gataaccgaa aaatcatcgg ttagaaaagc
    12481 ataagctaag gtagaaaagg aaaatgtggt aaaagcagct tgcaaataag tgaaaagcgg
    12541 agccaatgcc agccaatgat attgcttgcg aacttcgcct agaagtgcaa aaccaacttg
    12601 aaaaatggca ctaattgcag ccaataaaag tgaaaaatag ccgagttccg gtagcataaa
    12661 aaatccaaaa aatgaccgct tgtgaatgta aaaaaagccc tctccacttg tgggagaggg
    12721 acagattttt cttcgttcag aagaaaaatc agggagaggg gaattaaata attttgctaa
    12781 aaataacaat actccctctc tctgaaaaat atttctgaac gaaatatttt tcggtctcaa
    12841 tactcgctac gctcgctcct tcggagccgt tggcaaagcc aacgttcaaa tgtaaacatt
    12901 tgtcccacaa ggggagagag tatatcctaa cttgcaaccg ttagttgccc tgcataaagg
    12961 ataaagtaac gcaagcagag tacaccgatt aagtcgaaaa cggaaattaa gataataaat
    13021 ttcacgttat gtttcaagcg gtggctacca aaaatattgg caacaagcgg aatcgcaata
    13081 ccgattaaca ttacgcctat ccagaacaca ttcccccaga agccgttaag tgcatgggtt
    13141 aaagcgagcg atttatctgc tccaccgaag tgtaagccca caaagaacgc tacaattaag
    13201 cctaattcgg ttaccataat cggcacttca aatttgtgca taaagtggac ttcgtggctt
    13261 tcaccggata acttgcctgc gagtaaaatc actaggaaag ttgctgcaat acctgacgat
    13321 gcccctgatg ccaagaacaa tgccggtaac acagggttat tcagcattgg ataactcact
    13381 aacgccgaga gtaagaaacc ggtgtaagca ccaagtacag cagccaatac gaataagata
    13441 aattcaattg gttttaacag cttcgcttcg gcaaaatcga tcaaaccaga gaccacaggt
    13501 agataccgaa caatccaatc tttaaacatt atggcgatcc aaaccaccaa tactgccata
    13561 taaagttgga ataacattac ccccatcgac attactgagg taaagttata gttgaacatt
    13621 aatttccaga atgtccaagg tttggttaag tggaaaatca gtaacagtaa gccgataatg
    13681 gttggtaatg tgccaagaat agccgcacta cgaataatcc agttttggct tggtttttcc
    13741 attttgacac cgctattacg gtaagcaatc gctaactgaa ccgcacctga tgaaatacca
    13801 agtaggaata agtaaatagc gatagtgcca tcccaaacaa ggtttggggt ttggaaaggt
    13861 acatattcat tcattatcta tgttctcctt taccaaatgg aatgtgatat aaattcggct
    13921 ccgtgcctaa tgccgttttg gtgcgataaa ccggattatt tttcacttta tggaaaatat
    13981 cgctacttgg atcattcata tcaccaaagg ttaaagcttt agtcggacaa gcctctacac
    14041 aagcaggctg tttgccttct gcaagattgg tatctcggca aaagttacat ttatccgccg
    14101 attttttctc agggtgaata aaacgtacac gatacgggca aaccgcaata cagtattggc
    14161 aaccgacaca caaatctgag tgtacatcaa taatcccggt ttcaggatcg ataaatgaag
    14221 caccggtcgg gcaaacactc acacaaggtg cattggtaca gtgttggcaa gattggcgga
    14281 aaaactcata ttgtacgttc ggaaactcac cgaaaggctc actacggata atttctaaac
    14341 gagaaacccc ttccggcact ttgttggtgg tgcgacaagc gtccatacaa gccgtacagc
    14401 cgatacaggc agtttcatca tgaaccatcg catagcggat tttcttttgt tctttctcag
    14461 tagcaagagt tatggaggtg gcaagccctg tacttgccac aactgcgata gcacctgccc
    14521 ctgagacgaa atctctacgg gtgcaagtca ttattttgtt ccttttgctt ctgtggcttt
    14581 catttcagga tttttaaatt cgcctgaatg aattttggta tgacaatccg tacaaagttt
    14641 aatgcgatct ttttcaggaa tgtctttcat cggatctttt tccgggtgaa tttcatgaca
    14701 gttggtacaa gcaattttag ttgcgtgcgt gtcgtgagcc cagaattttt cacgcaattt
    14761 ctctgccgta tggcaagcaa aacagacttg attttgctca tttaccgaac gttcaagtga
    14821 tgggttactt gctttaccgt gtgggttaaa acgcattacg tcttttgccc ctttgcggtg
    14881 gctttcagaa atattaccgt ggcaacttat acaagtgagt ggctctccat tattcgggct
    14941 tttcacatct ttttggaaat gtttgcctgc gtgatggaaa ggcttacctg ttgcgtttgc
    15001 cgattcgtga cattgagtac aatatttatt tggatcacgg cgtgcatctt gagctacttt
    15061 tgcatcatca gccgtttctg attgccaaag aggttttggc tgctcaggca ttaacgcatt
    15121 cgcactggaa attgcaaaaa aacctgcaaa tacgaccgct tgtatgaggg cgactgctcg
    15181 tcctgctttt gagattaatt ttttcatagt tcacctcaag ctacattatt ttgcttctgc
    15241 ttttggtgct gctaccggtt tgtctgcttc ttctgcgggt aataaacctt tagcacgagc
    15301 ttctttttcc cattgaggaa ccacagtttt taagaactca tcttttgctg cttgctcttt
    15361 aggaatatct aaaccaatag caagttgtgc tttctcttta gttgagatgt ccggaatcgc
    15421 cactggcgtt gttacaccgt gtttcgctaa cactacacct aatttagcac gggcatctgc
    15481 tgcacggtcg atacctgttg cgatggtacg taacattaca tcaggtgcgt ggaagtggtt
    15541 accgtgacct gccgcagagt aatcccaacg ccattgagcg tgacgaattg ccattaagat
    15601 ctctttcatc tcttcttgag ttgctccagc ttcccaagca gctttcgctt caaagtgagc
    15661 tttcactaat tgatcttcta agcgaatcat tgcctctttt acttcgtgtt tatactcttt
    15721 tacacgtttt tccaatgttt ctttgctttg ttcatgacaa gttttacaag tcgcttcaaa
    15781 ttggtcaaac ggattaccga ttttgtggtc agtataaact ttgccgtctt tgtctttcac
    15841 ttttggcata tggcaatcaa tacaggttac accatttttc ccatgtgtac cttgtgacca
    15901 aacttcaaaa tctgggtgct gcgtttttag catcggagct ttcgataaag catgagtcca
    15961 gtctgtaaaa ttgatttcat cgtagtattt ttcaatgcta tctacatcta cacctttatc
    16021 ccaagggaaa gttacatctg ttttattttt gaaatagtac tccacatggc agttagcgca
    16081 cacagcaggg cgtttaccat gtttatctaa atcctcaaac ttccaaccaa ccgtattcaa
    16141 tgcacgtaat acgtgaggac gtgcaacacg tagtgcaggt ttaccttgtt taaactcttc
    16201 tgatgtcgtg tcatggcagt ctgcacaacc tattgagttt acaatttcag caccatattt
    16261 cgcccattta ggatcaaaat atcctcgctc acctttctcc tcaattaagc gtggaacatc
    16321 agggccttta catgtccaac aagccatagg ttgtggacca tcattttcat ctttaggcgc
    16381 gccggtgcgc aaaatatcac gcacatcggt aactgcataa aagtgacctc gtggtttatt
    16441 ataatctttc gcaaaagcat agcctgccca caaaataaca taacgagggt cagcctctaa
    16501 cgcacttcct cgatctgtgg attttgcagt tgacttccaa gaatcgtatt gtagcggata
    16561 tttttctgca aataggtggt ttgcagactc aattttcacg cctaattttt catgcttaac
    16621 tgctttatct gttaatgatt cagttttcgc ctcttcagct aaagcagttt gtaaacaccc
    16681 caatattgaa attgttgcca cagctaggct ttttattaaa aatttcattt tggctctcc
//
LOCUS       DANLYI010000035        16609 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.035, whole genome shotgun sequence.
ACCESSION   DANLYI010000035 DANLYI010000000
VERSION     DANLYI010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 16609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16609
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.035"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            <1..1706
                     /gene="argS"
                     /locus_tag="PXD81_000838"
     CDS             <1..1706
                     /gene="argS"
                     /locus_tag="PXD81_000838"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011200504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HDL5536887.1"
                     /translation="DKIKQAMVASGADSNVEPLVRQSGKPEFGDYQANGAMGAAKKLG
                     MNPREFAQKILENADLNGIADKLEIAGPGFINIFLNPEWLAENTNSSLQAVNFGIKTA
                     NPQTIVIDYSSPNVAKEMHVGHLRSTIIGDAVVRTLAFLGNNVIRANHVGDWGTQFGM
                     LIAYLEKMENEHASEMELSDLEAFYRAAKEHYDSDEAFAEKARNYVVKLQSGDKYCLT
                     MWKKLVDITMHHNQENYNRLNVTLTEKDVMGESLYNPMLPEIVADLKAKGLAVEDDGA
                     LVVFLDEFKNKDGDPMGVIVQKKDGGFLYTTTDIAAAKYRYETLKADRALVFSDSRQA
                     QHMQQAWLITRKAGYVPDSFSLEHPFFGMMLGKDGKPFKTRSGGTVKLKDLLDEAVER
                     ADKLIAERSTELTAEEKAAVVEAVAIGSVKYSDLSKNRTTDYVFDWDNMLTFEGNTAP
                     YMQYAYTRIRSIFARANIDENALSGSIQLNEPKERALAVKLLQFEEALNGVAKEGMPH
                     ILCQYLYELAGSFSSFYEACPILNAEETTKNSRLKLAALTAKTLKQGLDLLGIKTVEK
                     M"
     gene            1896..3062
                     /gene="cgtA"
                     /locus_tag="PXD81_000839"
     CDS             1896..3062
                     /gene="cgtA"
                     /locus_tag="PXD81_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HDL5536888.1"
                     /translation="MKFIDEALIRVEAGDGGNGCVSFRREKFIPKGGPDGGDGGDGGD
                     VYLIADENLNTLIDYRFEKRYAAGRGENGRSAGCTGHRGQDITLRVPVGTRAIDNDTQ
                     EIIGDLTRHGMKMLVAKGGYHGLGNTRFKSSVNRAPRQKTNGTPGEKRDLKLELLLLA
                     DVGMLGLPNAGKSTFIRSVSAAKPKVADYPFTTLVPSLGVARVGADRSFVVADIPGLI
                     EGAADGAGLGIRFLKHLERCRILIHLVDIMPIDESDPAHNISVIESELYQYSEKLAEK
                     PIWLVFNKIDTIGEEEAAERAKDIAEQIGWEGDYYLISAATGQNVQALTRDIMDFIEA
                     NPREVEEENKAPEEVKFKWEDYHNEAMQNAVEEDWDDDWSEEDDEGIEFIYERH"
     gene            3147..3764
                     /locus_tag="PXD81_000840"
     CDS             3147..3764
                     /locus_tag="PXD81_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005603203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase family
                     protein"
                     /protein_id="HDL5536889.1"
                     /translation="MLSSTSCDLFNIPFFQFAQMKKYCPEEIPRIKAEYKTQWEIWKK
                     LHQEVSRQLGQPFAPPHIEKWCNGWQVRAHFFAYYKYEFNRNSAAILSVILNRRRLQV
                     SLDWHCYRADRSQINVHQYNQWWENLDREKFADFEIWHGSESEYDDFRTVSQMSEQAF
                     TLTNEEDFWCIGKNVEKDDLDNIDVVEFIYQTIRALLPLYEKCHQ"
     gene            complement(3811..5769)
                     /locus_tag="PXD81_000841"
     CDS             complement(3811..5769)
                     /locus_tag="PXD81_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HDL5536890.1"
                     /translation="MIAELGNYTLALALAISILLAVLPLWGAEKQNSTLIALARPMTW
                     AMFLALTVSFGSLFYLFAVNDFTVQYVVNNSNSRLPLQYRLSAVWGAHEGSLLLWVWL
                     LSLWSVGVATFTRKMPEDAVARVLGLMGVIGIGFLMFVIFTSNPFDRTFPNFPIDGKE
                     LNPMLQDVGLIFHPPLLYMGYVGFSVAFAFAIASLMTGRLDTAWARWSRPWTMAAWVF
                     LTLGIVLGSWWAYYELGWGGWWFWDPVENASLMPWLAGTALIHSLAVTEKRGTFKAWT
                     VLLAILAFSLCLVGTFLVRSGILVSVHAFASDPTRGLYILAYLVLVVGGSLLLYAFKG
                     SEIKSLDNYERYSRETMLLINNILLMAFLSVVFLGTLLPLIHKQIGLGTISIGAPFFD
                     QMFLILMVPFSFVLGIGPLVKWRRDQVSAIRKPVIIATILMALLGFGLPYLFRNTITV
                     SVVLGTMMSVFIVLLSLYELHQRATHRHSFLSGIFKLSRSHWGMVLAHLGVAMTVFGI
                     AFSQNFSIERDVRMNVGDKAEILDYQFEFKGIKITDGANYQGGTAELEITRNGKYEAT
                     LNAEKRFYTVSRMGMTEAAIDWGFTRDLYAALGEKLEDNSWAVRLYYKPFIRWIWIGG
                     LFMALGGLLCMLDKRYRLRIENKKEVIA"
     gene            complement(5766..6323)
                     /gene="ccmE"
                     /locus_tag="PXD81_000842"
     CDS             complement(5766..6323)
                     /gene="ccmE"
                     /locus_tag="PXD81_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HDL5536891.1"
                     /translation="MNPRRKSRLKVVVSILLGLSVAAGLTLYALSQNIDLFYTPSEIV
                     NGKNDDPKQKPEVGQRIRVGGMVVEDTVKRDDKSLKVVFDLNDIGPSITVEYEGILPD
                     LFREGQGIVAQGVLIEPTRLKATEVLAKHDENYMPPELGDKLKEQHKKSGVSEQDLKG
                     ESETDRKMKAEAEAEKANNVQGESK"
     gene            complement(6509..6706)
                     /gene="ccmD"
                     /locus_tag="PXD81_000843"
     CDS             complement(6509..6706)
                     /gene="ccmD"
                     /locus_tag="PXD81_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HDL5536892.1"
                     /translation="MTFQFESISDFFAMGGYGFYVWLSYGVSFIAMIGLIWLSRREQK
                     AILAQAKKEIVREEKLKQTSQ"
     gene            complement(6721..7458)
                     /locus_tag="PXD81_000844"
     CDS             complement(6721..7458)
                     /locus_tag="PXD81_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter permease"
                     /protein_id="HDL5536893.1"
                     /translation="MWKWLHPYAKSETQYHLLGKIQPFLGWLSLIMLAAAFVWGLAFA
                     PKDYQQGDSYRIIFIHVPAAIWSMGVYGSMAVAGLVALVWQIRQANLAMISMAPIGLV
                     FAFISLATGAIWGKPMWGTWWVWDARLTSALVLFFLYIGVMALYSAFQDRQTGAKAAA
                     VLSVVGVINLPIIHFSVEWWNTLHQGATITKFDKPSMAVEMLIPLLLAIFGSLIFTAW
                     FSIWRYRIALLKDERNRPWVKQLAKSV"
     gene            complement(7658..8320)
                     /gene="ccmB"
                     /locus_tag="PXD81_000845"
     CDS             complement(7658..8320)
                     /gene="ccmB"
                     /locus_tag="PXD81_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005565617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HDL5536894.1"
                     /translation="MILLTIIQRELTIAFRKPSEILNPLWFFLMVITVFPILMGPNPE
                     LLGKIAGGIAWVAALLSALLSFERLFRDDYLDGSLEQLMLLPLALPQVALAKVIAHWL
                     LTGLPLILLSPIAAILLSLETHVWWALVLTLLLGTPILSCLGAIGVALTVGLRKGGTL
                     LSLLILPLFLPVLIFAAAVLEAATLNMGYSGQLAIIGAILAVTLTFSPFAIAGALRIS
                     LN"
     gene            complement(8317..8958)
                     /gene="ccmA"
                     /locus_tag="PXD81_000846"
     CDS             complement(8317..8958)
                     /gene="ccmA"
                     /locus_tag="PXD81_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HDL5536895.1"
                     /translation="MNQTAQYQLKLNQIACERGETRLFEGCSFTVQSGEWVQIEGHNG
                     IGKTSLLRILAGLFLPAEGEVLWNDIAIQKQRDHYYSELFYLGHYSGVKPELTPWENL
                     RFYQKIQQLPQDDDALWHALTKVSLIGREDLPCSHLSAGQQRRVALAKLWLTNAKLWI
                     LDEPFTAIDKKGVADLIAHIEQHCEQGRLVIFTSHQAAESDKVRIISLDQFKV"
     gene            complement(9102..9812)
                     /gene="hda"
                     /locus_tag="PXD81_000847"
     CDS             complement(9102..9812)
                     /gene="hda"
                     /locus_tag="PXD81_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015674467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA regulatory inactivator Hda"
                     /protein_id="HDL5536896.1"
                     /translation="MQLPLPIHQIDEDTFDNFYSQNSEVLLGSLKRNFTDVQQPFFYI
                     WGGKSCGKSHLLKAVSNHFLLNNQTSSYIPLTKANYFSPLVLDNAELLNVICLDDIQA
                     IAGDAEWELAIFNLFNQIREQQGLFNEGQKTLLLISADCPPHQLNIQLPDLRSRLTWG
                     EVYQLNDLTDEQKCQILQLNAHQKGVELSDEVAHFLLKKAGTDLEQLSKILDKLDKAS
                     LQAQRKLTIPFVKEILSL"
     gene            10082..11683
                     /locus_tag="PXD81_000848"
     CDS             10082..11683
                     /locus_tag="PXD81_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005616566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL5536897.1"
                     /translation="MKNMTKLSLITLALAASSSVFAAPKTFVYCLEASPSALSPVLST
                     DGASFDVNALPIYNKLTDFEDGTTNVIPSLAEKWDISEDGKTYTFHLRKGVKFHDNKE
                     FKPSRELNADDVVFSINRQFDPNHPFHKVSGGNYEYFIGMDMQNIIDKVEKVDDHTVK
                     ISLKVPNAPFLANLAMDFNAVYSAEYAEQSLKAGKPERIDTNPIGSGPFQFVDYQKDA
                     TVRYKAFENYWQGTAKIDRLVFAITPDASVRLAKLQKGECHAMPYPNPADIEDLKKDP
                     NIELMSQSGMNIGYLALNSSIKPLDNQKVRQALNHAVNKQAIVDAVYQGAGQVAKNPI
                     PPTMWSYNEAVQDYDYNPEKAKALLKEAGFENGFDTELWAMPVSRPYNPNARRMAELI
                     QEDWKKVGVNAKIVSYEWGEYLKRLRQGEAATSMIGWTGDNGDPDNFLNTLLSCSAVE
                     AGSNYAKFCHKPFEEVVLGAAQESDKAKRTELYKQAQVIFKEQAPWITIAHSTVYFPV
                     RKEVKGYVMSPFSLHNFYKVDLADK"
     gene            11955..12959
                     /locus_tag="PXD81_000849"
     CDS             11955..12959
                     /locus_tag="PXD81_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005712947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HDL5536898.1"
                     /translation="MFSFILKRVLMVIPTFIAITLITFALVHFIPGDPIEIRMGERGL
                     SPEVHQQMMEQLGLNRPLFEQYISYIKGVLHGDLGNSFRNNEPVLKEFFTLFPATVEL
                     AFFALLWSLIFGIILGVIAAVKKESWISHTVTTLSLTGYSMPIFWWGLILILYFSTPL
                     GLPASGRLPSEYWIEAETGFMLIDTWNSDEPGAFLAAIKSLILPSIVLGTIPLAVVTR
                     MTRSSMLEVLGEDYIRTAKAKGLNTTRIVIVHALRNALIPVVTVVGLIVGQLLSGAVL
                     TENIFSWPGIGKWIIDAINSRDYPVLQGSVLIIATIIILVNLTVDLLYGIVNPRIRHS
                     "
     gene            13061..13954
                     /locus_tag="PXD81_000850"
     CDS             13061..13954
                     /locus_tag="PXD81_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009875437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HDL5536899.1"
                     /translation="MSGITAVNPPAPKTPLQEFWYYFCQNRGALIGLTFIAIVFVACV
                     FAPWVAPFDPIEQNRSALLLPPAWLEGGNSAHLLGTDDIGRDILSRIIYGSRLSVFIG
                     LVIVLLSCVFGVILGLLAGYYGGTLDIIIMRFVDIMLAIPSLLLTIGVVTILGPSLIN
                     AAIAIAIVSIPSYVRLTRASVLSEKNRDYVTASRVAGASVLRLMFVVILPNCLAPLIV
                     QMTMGISNAILELAALGFLGIGAQPPTPELGTMLAESRGFMQSANWLVTIPGLAILSL
                     VLAFNLMGDGLRDALDPKLKQ"
     gene            13965..14954
                     /gene="dppD"
                     /locus_tag="PXD81_000851"
     CDS             13965..14954
                     /gene="dppD"
                     /locus_tag="PXD81_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HDL5536900.1"
                     /translation="MALLTVDQLSVHFGETKTPFKAVDRISYEVNEGEVLGIVGESGS
                     GKSVSSLAIMGLIDFPGRVMAEKLYFNGNDLLALKPKEKQQIVGADVSMIFQDAMTSL
                     NPSYTVGYQIMEALKVHQGGSKASRRERAIELLTMVGIPDPESRLEVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIDLLLELQRKENMALILITHDLALVA
                     ESAHRIIVMYAGQVVEEGKAEQIFKAPLHPYTQALLKALPEFAEGKSRLQSLPGVVPG
                     KYDRPQGCLLNPRCPYATEKCRTIEPAMQIINGRQVKCHTPLNAQGEPIRIAV"
     gene            15082..16071
                     /locus_tag="PXD81_000852"
     CDS             15082..16071
                     /locus_tag="PXD81_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL5536901.1"
                     /translation="MKENNSLPPFLKAVDLKKYYPVKKGMFAKPKTVKALDGVSFTLE
                     RGKTLAIVGESGCGKSTLGRMLTMIESPTEGELFYNGQNFLVNDKETAQLRRKKIQIV
                     FQNPYSSLNPRKKVGSILEEPLLINTNLSAKERKEKVLEMMAKVGLKPEFYDRYPHMF
                     SGGQRQRIAIARGLMLQPDIVVADEPVSALDVSVRAQVLNLMMDLQEEMGLSYVFISH
                     DLSVVEHIADEVMVMYLGRCVEQGDTKTIFSNPRHPYTQALLSATPRLNPEQRRERIK
                     LTGELPSPLNPPKGCAFNARCRFATDRCYVERPELKTYQDGTRIACFVVEENN"
     gene            complement(16305..>16609)
                     /locus_tag="PXD81_000853"
     CDS             complement(16305..>16609)
                     /locus_tag="PXD81_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248476.1"
                     /note="Bacteria have multiple sigma factors which are
                     active under specific conditions; the sigma factor binds
                     with the catalytic core of RNA polymerase to produce the
                     holoenzyme and directs bacterial core RNA polymerase to
                     specific promoter elements to initiate transcription;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RNA polymerase subunit sigma"
                     /protein_id="HDL5536902.1"
                     /translation="DDEENHFFDNYGHWKEEYYPSSWQEEAVYSKEFWILFEVCLSNL
                     PAKQARVFMMREYLEATSDEICRVVNITSDNLYTLIYRARLQLQQCLSRKLTERNR"
BASE COUNT         4951 a         3673 c         3546 g         4439 t
ORIGIN      
        1 cagataaaat taaacaggca atggtagcaa gcggtgcaga cagcaatgtc gagcctttag
       61 tccgccaatc gggcaagccg gaatttggcg attaccaagc caatggtgcg atgggtgcag
      121 cgaaaaaatt ggggatgaat ccgcgtgaat ttgcccaaaa aatcttagag aatgccgatt
      181 tgaacggtat tgccgataaa ttagaaattg ccggtccggg ctttatcaac attttcttaa
      241 acccagaatg gctggcggag aacactaaca gttcattgca agcggtcaat tttggcataa
      301 aaactgcaaa tccgcagacg attgtgatcg actactcctc gccaaacgta gcaaaagaaa
      361 tgcacgtcgg gcatttgcgt tccaccatta ttggcgatgc ggtagttcgt acccttgcgt
      421 ttttaggcaa taatgtgatt cgtgccaacc acgttggcga ctggggtact caattcggta
      481 tgctgatcgc ttaccttgaa aaaatggaaa atgagcacgc cagcgaaatg gagctaagcg
      541 atttagaagc cttctatcgt gcggcaaaag agcattatga ttcagacgaa gcattcgccg
      601 aaaaagcccg taactatgtg gtgaaattac aaagcggcga taaatattgc ttaacaatgt
      661 ggaaaaaatt ggtcgatatt acaatgcacc acaatcaaga gaactacaat cgcttaaatg
      721 ttacgctcac cgaaaaagat gtaatgggcg aaagcctcta caatccaatg ttgcctgaaa
      781 ttgtggcgga tttaaaagcc aaaggcttag cggtggaaga tgacggtgct ttagtggttt
      841 tcctcgatga atttaaaaat aaagatggcg acccaatggg cgtaatcgtg cagaaaaaag
      901 atggcggttt cttatatacc actaccgata ttgcggcagc aaaatatcgc tacgagacct
      961 taaaagctga ccgagcctta gtcttttctg acagccgcca agctcaacat atgcaacagg
     1021 catggctcat tacccgcaaa gcgggctatg tgccggacag tttcagcctt gaacacccgt
     1081 tcttcggaat gatgctaggc aaagacggca aaccgttcaa aacccgtagc ggtggcacag
     1141 tcaaactcaa agatttatta gatgaagcgg tagagcgtgc cgataaactg attgccgaac
     1201 gttcaaccga gctaactgcg gaagaaaaag ccgccgtggt tgaagcggtt gcgattggtt
     1261 cggtgaaata ttctgactta tcgaaaaacc gcaccaccga ttatgtgttc gattgggaca
     1321 atatgctgac ctttgaaggc aataccgccc cgtatatgca gtatgcctac acccgcattc
     1381 gctccatctt tgcccgtgcg aacattgacg aaaatgcatt gagcggttca atccaactca
     1441 atgagccaaa agagcgtgca ttagcagtga aactattgca atttgaagag gcattaaacg
     1501 gcgtggcaaa agaaggtatg ccacatattt tatgccaata tttatacgag cttgcaggta
     1561 gtttctccag tttctacgag gcttgcccga ttctcaatgc cgaagaaaca accaaaaaca
     1621 gccgtttgaa actcgctgcc ttaacggcga aaaccttgaa acaagggttg gatttactcg
     1681 gtattaaaac tgtagagaag atgtaataca aactctctcc ccttgtggga gagagaccgc
     1741 ttatgagcgt tcagcgaaat aagcagagag aggggacaag cggtaatttt tattaaaatt
     1801 tttacacatt ttcccctctc cctgattttt cttctgaacg aagaaaaatc tgtccctctc
     1861 ccacaagggg agagggcata taaaaggaaa caaatatgaa atttattgac gaagccctga
     1921 ttcgtgtaga agcaggcgat ggcggtaatg gctgtgtgag cttccgccgt gaaaaattta
     1981 tcccgaaagg cggacctgac ggtggtgatg gtggcgatgg tggtgatgtg tatttaattg
     2041 ccgatgaaaa cctaaacacc cttatcgact accgctttga aaaacgttat gccgctgggc
     2101 gtggcgagaa tggtcgcagt gccggctgta ccggacatcg tgggcaagat attaccctac
     2161 gagtgccggt tggaacccgt gcgattgata acgacaccca agaaattatt ggcgatttaa
     2221 cccgccacgg aatgaaaatg ctggtggcaa aaggtggtta tcacggttta ggtaataccc
     2281 gctttaaatc ctcagtaaac cgagctccac gccaaaaaac caacggtacg cctggcgaaa
     2341 aacgtgattt gaaattagag ctgttactgc ttgccgatgt gggaatgctt ggcttaccaa
     2401 acgccggtaa atccaccttt atccgcagtg tctctgctgc caaaccaaaa gtggcggatt
     2461 acccgttcac gacgctggtg ccaagtttag gggtagcccg tgtgggggcc gaccgcagtt
     2521 ttgtggttgc cgatattccg gggttaattg aaggtgcagc agatggtgca gggctgggga
     2581 ttcgtttcct aaaacacttg gaacgttgcc gtattttgat tcacttagtc gatattatgc
     2641 cgattgatga aagcgatccg gctcataata tttcggtgat tgaatccgag ctttaccaat
     2701 acagcgaaaa actcgccgaa aaaccgatct ggttggtgtt taataaaatt gacaccatcg
     2761 gcgaagaaga agctgctgag cgagcaaaag acattgccga gcaaatcggc tgggaaggcg
     2821 attactactt gatttctgcc gctaccggtc aaaacgtgca agctctcact cgtgatatta
     2881 tggactttat tgaagccaac ccacgcgaag tggaggaaga aaataaagca cctgaagaag
     2941 tcaaattcaa atgggaagac taccacaacg aagcaatgca aaatgcggta gaagaagatt
     3001 gggacgacga ttggagcgaa gaagatgacg aaggcataga gtttatttac gaacgccatt
     3061 agtgcaataa tacaagcggt tgtttttaaa cggagatttg caaatttccg gagaaaaaca
     3121 accgcttgta ggtttaagga gagtttatgc ttagctcaac atcgtgcgat ttattcaata
     3181 ttccgttctt ccaatttgct cagatgaaga aatattgccc tgaggagatc cctcgaatta
     3241 aggcggaata taaaactcaa tgggaaattt ggaaaaagct acatcaggaa gtttctcgtc
     3301 agcttgggca gccgtttgct ccaccacata ttgagaagtg gtgcaacggt tggcaagttc
     3361 gagcccattt ttttgcctat tacaagtatg aatttaatcg aaattctgcg gctattcttt
     3421 ccgtgatttt aaatcgtcgc cgtttgcaag tgagccttga ttggcattgt tatcgagccg
     3481 atcgctcgca aattaacgtg catcaataca atcaatggtg ggaaaattta gatcgggaaa
     3541 agtttgctga ttttgagatt tggcacggaa gtgagagtga atatgatgat tttagaaccg
     3601 ttagtcagat gtcagagcaa gcgttcaccc taactaacga ggaagatttt tggtgtatcg
     3661 gcaaaaatgt ggaaaaggac gatttggata acattgatgt agtagaattt atctaccaaa
     3721 ccattcgggc attattgcca ctttatgaga aatgtcatca ataaacacta aaaagagagg
     3781 cgaacatcat ttgttcgccc ctttcaaaat ttaagctatc acttcttttt tattttcaat
     3841 gcgtaaacgg tagcgtttat ccaacataca gagtaaacca ccgagagcca taaacagccc
     3901 gccaatccaa atccagcgaa tgaacggttt ataatacaaa cgcactgccc aagagttatc
     3961 ttctaatttt tcgccaagtg cagcgtataa atctcgggta aagccccagt caatcgccgc
     4021 ttcggtcatt cccattcggc tcaccgtata gaaacgtttt tcagcattaa gtgtggcttc
     4081 atatttaccg tttcgagtaa tttcaagctc ggcagtgccg ccttgatagt ttgccccatc
     4141 ggtgatttta atacctttaa attcaaattg gtaatctaaa atctcagctt tatcacccac
     4201 attcattctg acatctcgtt caatgctgaa attttggcta aatgcaatgc cgaacactgt
     4261 catcgcaacc cctaagtgag ccagcaccat tccccaatgt gaacgggaca gtttgaaaat
     4321 gccacttaag aacgagtggc gatgggttgc acgttggtgt agctcataca ggctaagtaa
     4381 cacaataaag acagacatca tcgtgcctaa caccacactc acggtaatgg tgttacggaa
     4441 taagtatggc aatccgaatc caagcaatgc cattaaaata gttgcaataa ttaccggttt
     4501 acgaatagca gaaacttggt ctctacgcca tttcactaat ggtccaatcc ctaatacaaa
     4561 tgagaaagga accattaaaa ttaagaacat ttggtcaaaa aatggtgccc cgattgaaat
     4621 cgtccctaag ccgatttgtt tgtgaattaa tggcagtaag gtccctaaaa acaccaccga
     4681 taaaaatgcc attaataaaa tattattaat gagtagcatc gtttctcttg aatagcgttc
     4741 atagttatct aagcttttaa tttccgagcc tttgaatgca tacagcagta aagaaccacc
     4801 tactactaac accaagtaag ccaagatgta taaaccacga gttggatctg aagcaaaggc
     4861 gtgaactgac accaaaatgc ctgaacgcac taagaaagtc cccactaaac agagcgagaa
     4921 agcaagaatc gctaataaga ccgtccacgc tttaaatgtg cctcgttttt cagtcactgc
     4981 taatgaatga atcagtgctg ttcctgcgag ccacggcatt aatgaggcat tctcgacagg
     5041 atcccagaac caccagccac cccaaccaag ttcgtagtat gcccaccaag aaccaagcac
     5101 gataccgagc gttaaaaata cccacgctgc cattgtccac ggacgagacc atcttgccca
     5161 cgcagtatct aaacgccctg tcatcagtga ggcaatcgca aaagcaaagg caacggagaa
     5221 acccacatag cccatataaa gcaacggtgg gtggaagatc aagcccacat cttgcagcat
     5281 tgggtttaat tctttgccat caatcggaaa atttgggaaa gttctatcaa acgggttgga
     5341 ggtaaaaatc acaaacatca aaaagccgat gccaatgacc cccattagtc ctaacactcg
     5401 agcaacggca tcttccggca ttttacgggt aaaggttgct accccgacag accaaagcga
     5461 caacaaccaa acccaaagca ataatgagcc ttcgtgagcg ccccacaccg cagataaacg
     5521 atattgcaac ggtaaacggc tattggagtt gttcaccaca tattgcacgg taaaatcatt
     5581 caccgcaaac agataaaaca gcgaaccaaa agagacagtc aatgccaaaa acattgccca
     5641 agtcatcggg cgggcaagag caattaaggt gctattttgc ttttctgctc cccaaagcgg
     5701 taaaactgcc aacaagatag aaatcgctag ggctaatgcc aacgtataat tgcctaattc
     5761 tgcaatcatt tgctttcccc ttgcacattg ttggcttttt ctgcttccgc ctcggctttc
     5821 attttgcggt cggtttccga ttcacctttc agatcttgtt cagacacacc tgatttttta
     5881 tgctgttctt ttaatttatc gcctaattcc ggtggcatat agttttcatc gtgttttgcc
     5941 agaacttccg tagcttttaa acgggttggt tcaatcaaca cgccttgtgc cacaatgcct
     6001 tgcccttcac ggaataaatc cggcaaaatt ccttcgtatt ccacggtaat cgaaggcccg
     6061 atatcattga gatcaaatac tacttttaaa cttttatcat cacgctttac cgtatcttcc
     6121 actaccattc cgcccacacg gatacgctga ccaacttcag gcttttgttt cggatcatca
     6181 tttttaccgt ttacaatttc agacggcgtg taaaacagat cgatattttg gcttaacgca
     6241 taaagtgtca agcctgctgc gacagaaagc ccaagtaata tcgaaaccac cacttttaat
     6301 cttgatttgc gtcttggatt catcgtaatc acccttcaaa ataaatgcaa aattgcataa
     6361 aaactcaaaa ataaattagt gtaagaatag caaaaaaccg caaaacattc ccgatatttc
     6421 aaggaagttt tgcgatttct caaatcaaat acctataaag aagtatgagg ctatcgttgc
     6481 aaaaaataaa gttatttcga ccgcttgttt attgtgatgt ttgctttaat ttttcttcac
     6541 gcacaatctc tttttttgct tgagccaaaa tcgctttctg ttcacggcga ctaagccaaa
     6601 tcagcccaat cattgcaatg aaactgacac cataagagag ccatacataa aagccgtagc
     6661 cacccattgc aaaaaaatcg ctgattgatt caaattgaaa tgtcattgtt ctctcctact
     6721 ctaaactgat tttgccagtt gttttaccca agggcgatta cgttcgtcct ttaataaggc
     6781 aatgcgataa cgccaaatgc taaaccaagc ggtaaaaatt aagctaccga aaatcgccaa
     6841 tagcaacgga attaacattt ccaccgccat agacggttta tcgaatttag tgatggtcgc
     6901 cccttgatgc aaggtgttcc accattccac cgaaaagtga ataatcggca agttaatcac
     6961 gccaacaact gacagcacag ccgccgcttt ggctccggtt tgtctgtctt ggaatgcgga
     7021 ataaagagcc atcacgccga tataaaggaa aaataacact aaagcggagg ttaaacgagc
     7081 gtcccacacc caccaagtcc cccacattgg tttgccccaa attgcaccgg ttgcaagtga
     7141 aataaaagcg aatactaagc cgataggtgc cattgaaatc atcgccaagt tagcttggcg
     7201 aatttgccaa accaatgcca ccaagcctgc caccgccatt gagccatata cgcccatcga
     7261 ccaaattgct gccggcacgt gaataaagat aatgcgatag ctatcgccct gttggtaatc
     7321 tttcggggca aaagccaagc cccaaacaaa agcagccgct aacataatca aacttagcca
     7381 acctaaaaac ggttggattt tccctagcaa atggtactgc gtttccgatt ttgcataagg
     7441 gtgtagccat ttccacatat atttctccaa agtaaataat tcccctcacc cgtttacagg
     7501 agagggacga tttgagcttc gttcagaagc gaaaatcagg gagaggggaa aaagttgcaa
     7561 attttcataa taattcgacc gcttcccctc tctctgaaaa acacctctga acgaggtgtt
     7621 tttcggtctc tctccctcaa gagggagaga ggaagtttta gttcaaacta attcttaatg
     7681 caccggcaat cgcaaacggc gaaaaagtta aagtaaccgc taaaattgct ccgataatcg
     7741 ccagttgtcc gctataaccc atatttaagg tggcggcttc taataccgca gcggcaaaaa
     7801 tcagtaccgg taagaataac gggaggatta ataaactcag caacgtaccg cccttgcgta
     7861 agcccacagt gagtgccacg ccaatcgctc ctaagcagct taaaatcggg gtgccgagta
     7921 ataatgtcag cactaacgcc caccaaacgt gggtttcaag tgaaagcaaa atggcagcaa
     7981 tcggtgaaag taaaatcaac ggtaagccgg tcagcagcca gtgagcaatc actttcgcta
     8041 acgcaacctg cggcaaggca agcggcagta acattaattg ctcgagcgaa ccgtctaaat
     8101 aatcatcacg gaataaccgc tcaaaggaaa gcaatgcaga aagcaaagca gctacccacg
     8161 caatgccgcc ggcaattttg cctaataatt cggggttagg tcccattaaa atcgggaata
     8221 cggtaatcac cattaaaaaa aaccacaacg gatttaaaat ctccgagggt ttacgaaagg
     8281 caatggtgag ttcccgctga ataatagtca ataaaatcat actttaaatt gatctaacga
     8341 aataatacgc actttgtcgc tttcagcagc ctggtggctg gtaaaaatca ctaatctgcc
     8401 ttgctcgcaa tgctgttcaa tatgagcaat cagatccgcc acaccctttt tatcaatagc
     8461 ggtaaagggt tcatctaaaa tccaaagttt ggcgttagtg agccacaatt ttgccaacgc
     8521 cacacggcgt tgctgaccgg ctgacaaatg cgaacaaggc aaatcttcac gcccgatgag
     8581 cgacactttc gtcaatgcgt gccaaagggc atcatcatct tgcggtaact gttggatttt
     8641 ctggtagaaa cgcaaatttt cccacggtgt taattccggt tttacccccg aataatgccc
     8701 taaataaaat agttcagaat aatagtgatc acgctgtttt tgaattgcaa tatcgttcca
     8761 cagcacctca ccttccgcag gtaaaaaaag cccggctaaa atacgtaaca ggctggtttt
     8821 tccaatgccg ttatgccctt caatctgcac ccattcacca ctttgcacgg taaaactgca
     8881 cccttcaaac aaacgggttt cgccccgttc gcaggcaatc tgattaagtt taagctgata
     8941 ttgggccgtt tggttcatta tttttcatct ggttgaacat tttagccgaa gtgtatcata
     9001 agcctttgat ttaacgtagc aaataaatta cttctttaga ggtagcaagc ggtcaaattt
     9061 tcaaaaagtt ttgcaaattt tgataagaat caaaccgctt gttacagaga gaggatttcc
     9121 ttcacaaaag gaatggttaa tttacgctgg gcttggagag acgctttatc caacttatcc
     9181 aatattttag agagttgctc taaatccgtt cccgcttttt ttaacagaaa atgagcgacc
     9241 tcatcggaca attccacccc tttttggtga gcatttaact gtaaaatttg gcatttttgt
     9301 tcatcggtta aatcgtttag ctggtacact tcgccccaag ttaagcggga acgtaaatcc
     9361 ggcaattgga tattgagttg gtgtggtggg caatcggcac tgattaacag caaggttttt
     9421 tgcccttcgt taaacaagcc ttgttgttct cgaatttggt taaacaggtt aaaaatagca
     9481 agttcccatt cagcatctcc tgcaatcgct tgaatatcat ctaggcaaat cacattcagc
     9541 agttcggcat tatctaacac caacggcgag aaataattgg ctttagtgag cggaatataa
     9601 ctagaagttt ggttatttaa aagaaagtgg ttacttactg ctttcaacaa atgactttta
     9661 ccgcagctct taccgcccca aatgtaaaaa aacggctgtt gaacatccgt aaaatttcgt
     9721 ttgagggatc caagcaacac ttcgctgttt tggctataaa aattgtcgaa agtgtcttca
     9781 tcaatttgat gaatgggtag aggtaactgc aaggctttcc ttaaatctct aagtttgata
     9841 tttttctaag aatagcggaa aagcaggggt ttgagaagta aaaatagtga gaaaatatcc
     9901 actcattttt ctaatgaaga gatgacaagc ggttgaattt ccacataatt ttgtcaatct
     9961 gaccgaaaac ccaccgtttt gtgttaaaaa aagattgcag ttagctcaaa aaattgtata
    10021 ttcaaagctt tctacttaaa aagtagagta aaacaactat tacaaacaca acggagtgaa
    10081 tatgaaaaac atgactaaat tatcgctaat aacattagcg ctagccgctt caagtagcgt
    10141 ctttgctgca ccgaaaacct ttgtttactg cttagaggct tcgccttcag cattaagccc
    10201 agtgctatca acagacggtg caagctttga tgtgaacgcc ctcccgattt ataacaaatt
    10261 aaccgacttt gaagacggta caactaatgt tattccctct cttgccgaaa aatgggacat
    10321 ttcggaagac ggcaaaactt acaccttcca cctgcgtaaa ggcgtaaaat tccacgacaa
    10381 taaagaattt aaaccaagcc gtgagttaaa tgcagacgat gtagtgttct ccattaaccg
    10441 tcagtttgat ccaaaccatc cattccacaa agtttccggc ggtaactacg aatactttat
    10501 cggtatggat atgcaaaata ttatcgataa agtggaaaaa gtggacgatc acaccgttaa
    10561 aatcagctta aaagtgccaa atgcgccgtt cttagctaac ttagcgatgg atttcaacgc
    10621 cgtttactca gcggaatatg cagagcaatc cttaaaggcc ggcaagcctg agcgtattga
    10681 taccaaccca atcggcagcg gcccgttcca gtttgtggat taccaaaaag atgccaccgt
    10741 tcgttacaaa gcctttgaaa actactggca aggcacagcg aaaattgacc gcttagtgtt
    10801 tgcaattact ccggatgcat cggtacgttt agccaagtta caaaaaggcg agtgccacgc
    10861 aatgccttac cctaacccgg ctgatatcga agatctgaaa aaagatccaa atattgaatt
    10921 aatgagccaa tctggaatga atatcggcta tttggcatta aattcatcga ttaaaccatt
    10981 agataaccaa aaggtacgtc aagcattaaa tcacgctgta aacaaacaag cgattgtaga
    11041 tgcggtatat cagggagccg gtcaagtagc gaaaaaccca attccaccaa caatgtggag
    11101 ctataatgaa gcggttcaag actatgacta caaccctgaa aaagccaaag cattgttaaa
    11161 agaagccggt tttgaaaatg gttttgatac tgaactttgg gcaatgccgg tatcacgtcc
    11221 atataacccg aatgctcgcc gtatggctga acttatccaa gaagactgga aaaaagtggg
    11281 cgtgaatgcg aaaatcgtca gctatgaatg gggcgaatat ctcaaacgct tacgccaagg
    11341 tgaagcggca acctcaatga ttggctggac aggcgataac ggtgacccag ataacttcct
    11401 caacacctta ttaagctgct cagcggtgga agccggttca aactacgcca aattctgcca
    11461 caaaccgttt gaagaagtcg tgcttggtgc tgcccaagaa agcgacaaag caaaacgtac
    11521 cgagctttat aaacaagctc aagtgatctt caaagagcaa gctccttgga ttacgattgc
    11581 tcactccacc gtttacttcc cggtgcgtaa agaagtaaaa ggttatgtga tgagtccgtt
    11641 cagcttacat aacttctaca aagtagattt agcggataaa taatccgcca acaagcggta
    11701 gaatttgcag atttttttac aaatttgacc gcttgtcctc ccctctttga actacgaggg
    11761 gcagggggga aatttggcag aagcgattgc cgaagtgaga ggattgtaaa agaggaaata
    11821 aattttctgt cttattgatt attttcttcg ttaattacta ctgtcaaatc tcccctaccc
    11881 cctctttgct aaagagggga atcagatcga gagatcagac aactttgatg aaaatccaac
    11941 ctaagagatt ttaaatgttt tcatttattt taaaacgtgt gctaatggtg atccctacct
    12001 ttatagcgat tacgttaatt acttttgccc ttgtgcattt tatccccggt gatccaattg
    12061 agatccgaat gggggaacgt ggattatcgc ctgaggttca tcaacaaatg atggaacaac
    12121 tcgggctgaa ccgaccgttg tttgaacaat acatcagcta catcaaaggt gtattgcacg
    12181 gtgatcttgg taactcattt cgtaacaacg aaccggtgct gaaggaattt tttaccctat
    12241 tcccagccac ggttgagttg gcattttttg ccctgctctg gtcgttgatt ttcggcatta
    12301 ttctcggtgt gattgctgcg gtgaaaaaag aaagttggat ttcacacact gtaaccacgc
    12361 tttcactgac cggctactca atgccaattt tctggtgggg cttgatttta attctttatt
    12421 tctccacccc gctcggctta ccggcttcag gtcgtttacc atctgaatac tggattgaag
    12481 ccgaaaccgg ttttatgctg attgatactt ggaattcgga cgaaccaggg gcatttttag
    12541 cggcaattaa atctctgatt ttgccgtcta tcgtactggg gacaatccct cttgcagttg
    12601 tgacccgaat gactcgctct tcaatgttgg aagtgttggg tgaagactat atccgcaccg
    12661 ccaaagccaa aggcttaaac actacccgaa ttgtgattgt tcacgcatta agaaatgcct
    12721 tgattccggt ggtaacggtc gttggcttaa ttgtcgggca actgctttca ggagccgttt
    12781 taacggaaaa catcttctcg tggccgggta tcggcaaatg gattattgat gcaatcaaca
    12841 gccgtgatta ccctgtacta caaggctcag tgctgattat tgctactatt attattttag
    12901 tgaaccttac ggtagatcta ctctatggca ttgtcaatcc gaggattcga catagttaga
    12961 aaaattcctt cgatttttcc cctctttagc aacgagggag ttacacaagc ggttcaaatt
    13021 ccgcaatttt ttacaagatt atttttagtt ggagttaatg atgtcaggaa taacggcggt
    13081 aaatccaccc gcacccaaaa caccgttaca ggagttttgg tactattttt gtcaaaatcg
    13141 gggggcatta ataggactta cctttattgc aatcgtcttt gttgcgtgcg tattcgcacc
    13201 ttgggttgcc ccttttgatc caatcgaaca aaatcgcagt gcgttattgc tccctcctgc
    13261 ttggcttgaa ggggggaatt ccgctcatct gctgggtact gacgatattg ggcgtgatat
    13321 tctttcccgt attatttatg gttcaagatt gtccgtcttt atcggcttag tcattgtgct
    13381 actttcttgc gtattcggcg tgattttagg tctacttgct ggctactatg gtggcacact
    13441 cgacatcatc attatgcgtt ttgttgatat tatgctggcg attccaagcc tgcttttaac
    13501 tatcggcgtg gtcactattt taggcccttc attgataaat gccgctattg cgattgcgat
    13561 tgtctccatt ccaagttatg ttcgcttgac ccgtgcttct gtattaagcg agaaaaaccg
    13621 tgattatgtt actgcttctc gtgttgctgg tgcgagcgtg cttcgcttaa tgtttgtggt
    13681 gattttacct aactgtttag ctccgcttat cgttcagatg acgatgggga tctcaaacgc
    13741 tattttagaa cttgccgccc tcggcttctt aggcattggt gcacagccgc caacaccgga
    13801 gcttggcaca atgcttgccg aatctcgtgg ctttatgcaa tcggcaaact ggctggtgac
    13861 cattccgggt ctagcgattt tatcgttagt attagctttc aacttaatgg gtgacggctt
    13921 gcgtgacgcc ctcgatccaa aactgaaaca ataggagatc taaaatggca ttattaacgg
    13981 ttgatcaact ttccgtccat tttggtgaaa ctaaaacacc atttaaagcg gtggatcgta
    14041 tcagttacga agtcaatgaa ggcgaagtat taggcattgt gggcgaatcc ggttcaggaa
    14101 aatcggtaag ttctctcgct attatgggct taatcgattt ccccggcagg gttatggctg
    14161 aaaaattgta ttttaacggc aatgatttat tggcattaaa accaaaagaa aaacaacaga
    14221 ttgtaggggc tgatgtctca atgatcttcc aagatgcaat gaccagctta aatccgagct
    14281 atactgtggg ctatcagatt atggaagccc taaaagtgca tcaaggtggc tcaaaagcca
    14341 gccgtcgtga gcgagcgatt gagttgctga cgatggtcgg tattcccgat cctgaatccc
    14401 gtttagaagt ttatccgcac cagctttcgg gtggtatgag ccagagagtg atgattgcga
    14461 tggcgattgc ctgtcgcccg aaattattga ttgccgatga gccgactact gcgttagatg
    14521 tgacgattca agctcaaatc attgatttat tacttgaatt acaacgcaaa gaaaatatgg
    14581 cgttgatttt aatcacgcac gatttagcgt tagttgccga gtctgcccat cgcattatcg
    14641 tgatgtatgc agggcaagtc gtggaagagg gcaaagccga gcagatattc aaagcaccac
    14701 tgcaccctta cacccaagca ttgctcaaag ccttaccgga atttgccgaa gggaaatccc
    14761 gcctgcaatc actaccgggc gtagtgccgg gcaaatatga ccgcccacaa ggttgcttac
    14821 tcaaccctcg ctgcccgtat gcaaccgaaa aatgccgaac cattgaaccg gcaatgcaga
    14881 taatcaacgg cagacaggtg aaatgccaca cgccactgaa tgcacagggt gagccgataa
    14941 ggatagctgt ataacgtcat aaagagtagg gacgcaatgc ttcgcgtccg ttacaagcgg
    15001 tgatttttaa ccgatttttg caaaataacg atagtggacg caaagcattg cgtccctaca
    15061 ttttccgaga aggaaaacag aatgaaagaa aataattctc tcccaccatt cctcaaagca
    15121 gtggacttaa aaaaatacta cccagttaaa aagggaatgt tcgctaaacc gaaaacggta
    15181 aaagcgctgg atggtgtctc ttttaccctt gaacgtggca aaacccttgc gattgttggc
    15241 gaatccggct gtggaaaatc aactttgggg cgaatgctca ctatgattga aagcccaacc
    15301 gaaggtgaac tgttctacaa cgggcagaat ttcttagtca atgacaaaga aacggctcaa
    15361 ctacgccgta aaaaaatcca aattgtgttt cagaacccgt atagctcgct caatccacgc
    15421 aaaaaagtag gtagcatttt agaagaacca ttattgatta acaccaactt atccgcaaaa
    15481 gaacgcaaag aaaaagtgtt ggaaatgatg gcgaaagtcg gcttaaaacc ggaattttac
    15541 gaccgttatc cgcatatgtt ttcgggcgga caacgccagc gaattgcgat tgctcggggc
    15601 ttaatgttgc agcctgatat tgtggttgcc gatgaacccg tttccgcatt agatgtttcg
    15661 gttcgggcgc aagtgttgaa tttgatgatg gatttgcaag aagaaatggg cttgtcttac
    15721 gttttcatct cgcacgattt atccgtagtg gaacatattg ccgatgaagt gatggtgatg
    15781 tatttagggc gttgcgtaga acaaggggat accaaaacta tcttcagcaa tccacgtcac
    15841 ccttatacac aagcgttact ctccgcaacc cctcgtttga acccggagca acgccgtgag
    15901 cggattaaac tgactggcga actaccaagc ccgcttaatc caccaaaagg ctgtgccttt
    15961 aacgcccgtt gcagatttgc aaccgatcgt tgctatgtgg agcgaccaga gctgaaaacc
    16021 tatcaagacg gtactcgcat tgcttgtttt gtggtggagg aaaataatta ggcaacgctc
    16081 gctatttaaa aggttaaagt ttttcaactt taacctttat ttttcatact taatactcct
    16141 catcttttgt agcaaaatct ttcattaatt tgctgatatg ctcacaatgg tgtttaaatg
    16201 acaatacggg caaataatca aatgcgtttt aacgccaatc ttctctttta aagtgagttt
    16261 tcgttccttt gagtcagaaa ttaaccttgt tacttgtcgg caattcatct atttctctcc
    16321 gttaatttcc ttgaaagaca ttgttgtaac tgcagccttg ctcgatagat taatgtatat
    16381 aaattatctg atgtaatatt tactactcga caaatttcat ctgaggttgc ctctaaatat
    16441 tccctcatca taaataccct agcttgtttt gcaggtaaat tgcttaagca aacttcaaat
    16501 aatatccaaa attctttgga ataaacagct tcttcctgcc agctagaagg ataatattct
    16561 tctttccaat gcccataatt atcaaaaaag tgattctctt cgtcatcat
//
LOCUS       DANLYI010000036        16373 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.036, whole genome shotgun sequence.
ACCESSION   DANLYI010000036 DANLYI010000000
VERSION     DANLYI010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 16373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16373
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.036"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            306..887
                     /gene="rsxA"
                     /locus_tag="PXD81_000854"
     CDS             306..887
                     /gene="rsxA"
                     /locus_tag="PXD81_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007525505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HDL5536903.1"
                     /translation="MVDYILLVISTALINNFVLVKFLGLCPFMGVSKKVETAIGMGLA
                     TTFVLTVASLSAYLMETYLLEPLNAQFLRTLVFILVIAVIVQLTEMIVHKTSPTLYRL
                     LGIYLPLITTNCAVLGVALLNVNLANNLVESVLYGFGAAAGFSLVLVLFAALRERLAA
                     ADVPRPFQGASIALITAGLMSLAFMGFTGLVKL"
     gene            887..1504
                     /gene="rsxB"
                     /locus_tag="PXD81_000855"
     CDS             887..1504
                     /gene="rsxB"
                     /locus_tag="PXD81_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HDL5536904.1"
                     /translation="MLDLPIIAYILIAIAVIALIFGAVLGYSSIKLKVESDPIVDQID
                     ALLPQSQCGQCGYPGCKPYAEAIANGDVITKCVPGGQPLVVNLAELLGVEVPPMEGMA
                     EPEMKVAFVIEDLCIGCTKCIQACPVDAIIGTNKAMHTIIPDLCTGCELCVAPCPTNC
                     IEMRPIKPTTQSWNWKFDQDLIIPIVNTTELQRKLVTGGGKNEQS"
     gene            1491..3785
                     /gene="rsxC"
                     /locus_tag="PXD81_000856"
     CDS             1491..3785
                     /gene="rsxC"
                     /locus_tag="PXD81_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HDL5536905.1"
                     /translation="MSNPNLVIERIRQNKETGKLWDFPGGIHPMENKSQSNSKPIRTL
                     NLPKFFYVPLVQHSGWAGELLVKVGDVVLKGQPLTKGDHFRQLPVHSPTSGRVIGIEP
                     YVSAHASGLPEITVIIETDGKDKWVERQPIEDFLSLTSDQIIQKVYQYGIAGLGGAVF
                     PTATKLSLADKRCKLLVINGAECEPYITCDDRLMQDFTADLIEGIRILRYVLRPEEVV
                     IAIEDNKPHAIQALETALKGSNDMFVRAIPTKYPSGASDQLIQVLTGLEIPAGKRTIE
                     MGIVMHNVGTAFAVKRAIIDDEPLIERVVTLTGDKIRNKGNVWARLGTPIQHLLEQVD
                     YQADKRFPVFLGGPMMGFILPALQAPITKTANCLIAPDHFEYAPPEPERSCIRCSSCS
                     DACPVGLLPQQLYWFARAEDHDKSKEYHLDACIECGVCAYVCPSYIPLIQYFRQEKAK
                     IEEIEEKARKAEEAKIRFEAREARLQKERDARTARISQAAEKRREEVANAGGVDPVKA
                     ALERLKAKKAVETVQEIPAEVQVKPNGEPDNSDLMAQRRARRLAKQAEQAVEKSENVT
                     NALIGENNDLPLQSMNETASDKSVGANNYSPEAESEDPRKAAVAAALARAKAKKQAQQ
                     AVEKSENITNAVIGVNNDLPLQSMNETVSDKSVGANNHSPETESEDPRKAAVAAAIAR
                     AKAKKQAQQAVEKPEDIANAVIGVNNDLPLQSMNETTSDKSVGANNHSPKTESEDPRK
                     AAVAAAIARAKAKKAAQQEKSNNV"
     gene            3778..4413
                     /locus_tag="PXD81_000857"
     CDS             3778..4413
                     /locus_tag="PXD81_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HDL5536906.1"
                     /translation="MFSELIPALPWSFLFIQDLIGTIVFAISGAIAARQHKMDIFGMF
                     ILAFVTGVGGGTLRDMMIGSTPVFWMKQPIYVLMITLAVVITAIFRNQISRKSWQTGL
                     LVFDAIGLGVFTVIGVQKGLDFGLHPLICVALGGVTGCFGGLIRDILRNEVPIILQKE
                     VYVTASLIGGVIFVAANSVGLESDWVYLATVFTVIIIRLLAIRFNLNLPRL"
     gene            4459..5514
                     /gene="rsxD"
                     /locus_tag="PXD81_000858"
     CDS             4459..5514
                     /gene="rsxD"
                     /locus_tag="PXD81_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HDL5536907.1"
                     /translation="MFKMVSSPHTHSSNLTANFMLWVIAMMLPAFGVQLYYFGYGVLI
                     QTLLAVSLAIVIEIGIAKLRKKTTAFYLADLSGILTALILAMSIPPYAPYWLVLIGVI
                     VALLLAKHSYGGLGQNLFNPAMVGYALLLVSFPVQMTAWIVPIELLSEPPTFRDAISL
                     IFYGVTSDGFSIHQLVNSVDGIAQATPLDSMKSSLAKSGFDAVMNTPIFNGLFATGWL
                     QLNLAFLFGGLVLIYKRIIHWQIPVAMLATFAFFSGFTELFSQQSHLHFVSHFFSGAM
                     MFGAFFIATDPVTASITPRGKLIFGGLVGLLVYLIRYYGNYPDAIAFSVLLANICVPL
                     IDHYTQPRLYGTKLDNR"
     gene            5514..6146
                     /gene="rsxG"
                     /locus_tag="PXD81_000859"
     CDS             5514..6146
                     /gene="rsxG"
                     /locus_tag="PXD81_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HDL5536908.1"
                     /translation="MNTSKITLNYALILGFIALLCTAVSTGVYLLTKGKIDDVTAMQQ
                     RKLLEEVVPKSHFDNDVLATCKLVQLENAPYLNRIYVAKKSENLTAYLVQGTAPDGYS
                     GDIVLLMGIEPNGNILGVRTLEHKETPGLGDKIETRVSDWILSFTNQLFSLENEADWN
                     VKKDGGKFDQFTGATITPRAVVNHIRQSAKWVVTELAQNPTMFNQFKACK"
     gene            6155..6838
                     /locus_tag="PXD81_000860"
     CDS             6155..6838
                     /locus_tag="PXD81_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005624031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit E"
                     /protein_id="HDL5536909.1"
                     /translation="MEREQQIPVTNIDVAPKQSSVWKTIFADGVWKNNGALVQLLGLC
                     PLLAVSNSVTNALGLGLATMFVLICTNITVSLFRKITPHDIRIPIYVMVIATVVTAVQ
                     LLMNAFAFPVYQSLGIFIPLIVTNCIVIGRAEAYASKNEVKFSAFDGFSMGLGMTFSL
                     VVLGALREIIGNGTLFSGLDLLLGDWAKALRIDVLHIDSGLLLAILPPGAFIGLGVIL
                     AVKNIIDKR"
     gene            7279..8229
                     /gene="lpxM"
                     /locus_tag="PXD81_000861"
                     /gene_synonym="msbB"
     CDS             7279..8229
                     /gene="lpxM"
                     /locus_tag="PXD81_000861"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249087.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HDL5536910.1"
                     /translation="MTRITAQDGYTHKFLWSFLHPKYWGVWLGVLGLVILAYVPVRIR
                     DKLSAFVGKMAYRYLKQKNKKGYHRAKVNLRYCFPDWSEKKREEVVEKMFITVAQTML
                     AIGETAIRPASYLQKRCEFTGFENVVKAKESGKNVIMLVPHTWSIDMAGVAMFSLGYP
                     MTSMYNPHRNALVDWLWNITRERLGGRMHTRQNGIKAFLADVRKGDVGYFLPDEDFGE
                     ELSVYADFFATEKATLPGLNKMARVANAEVIPMFSIYNAEKAKYEMEILPPLEFSGDP
                     IQSAREMNRVIEYFVEKNPEQYVWILRLLKTRRNREDIYL"
     gene            8357..8896
                     /gene="apt"
                     /locus_tag="PXD81_000862"
     CDS             8357..8896
                     /gene="apt"
                     /locus_tag="PXD81_000862"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HDL5536911.1"
                     /translation="MNQLDLIKSSIKSIPDYPKAGIIFRDITSLLEVPTAFQATIDAI
                     VAEFKDKGITKIVGTESRGFIFGAPVALALGVPFVLVRKPKKLPREVISQSYTLEYGE
                     DTLEIHKDAVSANDNVLVIDDLLATGGTIDATAKLIRRLGGNVEHAAFVIWLPELGGK
                     ERLEKEGVKSFSLVSFEGH"
     gene            8987..11044
                     /gene="dnaX"
                     /locus_tag="PXD81_000863"
     CDS             8987..11044
                     /gene="dnaX"
                     /locus_tag="PXD81_000863"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HDL5536912.1"
                     /translation="MSYQVLARKWRPQRFSEVVGQQHVLSALENSLREDRLHHAYLFS
                     GTRGVGKTSIARLFAKGLNCVNGITAEPCGECANCKAIEEGHFIDLIEIDAASRTKVE
                     DTRELLDNVQYKPTVGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEYVKFLLATTDPQ
                     KLPITILSRCMQFHLRALEQSQIARHLEFILNAENIPFEPLALEKLAKAAQGSIRDSL
                     SLTDQAIAMSNANITLAIVSQMLGLIDDNQPLELVQATAQADGEKAMAIIEQIAAKGV
                     DWQQLLSDVAEVLHKIAMLQLVKTPTTEETLVHFLAKQIPPEDVQFFYQLILNSKKEL
                     PFAPEQRAGVEMAILRALAFHPKQAEKIATVAVEKVTQPNHSVSAVTTAKPQNQISQL
                     RENFEQNQQKTVTVANTTTAVESIAQPPQAVDTAISPSAPATLSPAMAALEARKKLQA
                     QQANLAQTEKKKPELKAPQVIAKKSANQTASLQERFISLAENQHKAVLQVSKKTESAP
                     KSVEDEDYRWTWLNPELENQADSAKPSDIKQAILQERTPELVAKTIHLSCQQDKWCDI
                     ANSLNLGGFSRQIALNSYLAEQNEQHLALVLKPNMAHLDNPESRKNLEAALAEKGYSY
                     ELTIGDNSEQQTPLEIRRAIFKQLTEAAKSALLNDEKLMLLRNAFDAQIDESTIRAVA
                     ETE"
     gene            11050..11958
                     /locus_tag="PXD81_000864"
     CDS             11050..11958
                     /locus_tag="PXD81_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL5536913.1"
                     /translation="MKALELIDLVKTYPTGVKAVKGINLVVEQGDFYALLGHNGAGKS
                     TTIGIISSLVNKTSGSVKVFGYDLDTQKVELKHQIGLVPQEFNFNQFEKVIDVLVQQA
                     GFYGIPRSEALVRAEKWLKLLDLWEKRTHLTRELSGGMKRRVMIARALMHNPKLLILD
                     EPTAGVDIELRRSLWDFLRDLNNQGTTIILTTHYLEEAETLCRHIGIIQHGELIENTS
                     MKALLAKLESEVFVLDLQNSEQNQPLVIQNYPHKWLDENTLEVNVKRQQGLTNLFSQI
                     SAQGAEVLSMRNKSNRLEELFINMAQ"
     gene            11960..12721
                     /locus_tag="PXD81_000865"
     CDS             11960..12721
                     /locus_tag="PXD81_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007523083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL5536914.1"
                     /translation="MNLIGFYTLAYKECRRILRIWRQTLVPPVITTTLYFLIFGTLIG
                     GRIGEMNGVGYTQFIAPGLIMMTAITASYVNTASSFFLSKFTRDFEEMLISPLSTHNI
                     IWGYVTGSILRAGLAATLVMCVAMVFVSFEIYSVAIIVLTLLLTIIAFALGGVINAVF
                     ARSFDDVGLIPTFVLTPLTYLGGVFYSISLLPEFWQTVSKFNPIVYMINGLRYGFLGI
                     SDVPLGYTFAVLTSLCIGLYIIAYSLIQRGVGLRS"
     gene            12742..13497
                     /gene="modA"
                     /locus_tag="PXD81_000866"
     CDS             12742..13497
                     /gene="modA"
                     /locus_tag="PXD81_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HDL5536915.1"
                     /translation="MKALKKLAITMALTLSGTAFSQEITVFAAASMTNVLQDIQQDFE
                     KAYPQDKITFSFASSSVLAKQIEQDAPADIFISADQKWMDYVAEKQPEKVKHRRNLVR
                     NELVLIAPTESNIEPKAIQAVDFAKELANSYLSVGDPNHVPAGKYAKKALSHYQLWES
                     VEPRLARAKNVRDALSFVERGESPLGIVYSTDAKVSNKVKVVAVFPPESYGEIVYPAA
                     AVSEKASAATFLDFLKTPKAKAKFEAAGFYPVN"
     gene            13681..14373
                     /gene="modB"
                     /locus_tag="PXD81_000867"
     CDS             13681..14373
                     /gene="modB"
                     /locus_tag="PXD81_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HDL5536916.1"
                     /translation="MLNFTPQELNAIVLSLKVASIAVVLALPLAVWVAWLLARKQFWG
                     KNLLNGIMHLPLVLPPVVIGYLLLISMAKRGVIGQYLWEWFHFSLSFSWKGAVLASMV
                     MAFPLMVRSIRLAFEAVDPKLEQAARTLGAIPLKVFFTLTLPLSFSGIIAGGVLGFAR
                     SLGEFGATITFVSNIPNQTQTIPAALFTFIETPDGEMAAARLCLVAILISLIALFCSE
                     WLAERQKRYAQT"
     gene            14360..15415
                     /gene="modC"
                     /locus_tag="PXD81_000868"
     CDS             14360..15415
                     /gene="modC"
                     /locus_tag="PXD81_000868"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009875448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HDL5536917.1"
                     /translation="MLKLKLLQQLGNLTLNVNLDLPNKGITAIFGRSGAGKSSLINLI
                     AGLSTAQAGFIELNGQVLFDSQNGINLPPEKRNIGYVFQEHRLFPHYSVEKNLKYGYK
                     RSDSADFLQIVELLGIAPLLNRYPASLSGGEKQRVAIGRALLSSPNILLMDEPLSALD
                     LPRKQELLDYLDKLSARLEIPILYVSHSLEEVVRLADHLVLLENGKVTAFDTLSAVWH
                     SSAFSAWQPNHQRISLLELPVEETLPHYNMLGLSLNAQRLWVNQLPRHQIGEKLRVTI
                     ASKEVSISLQKPTQSSIRNVLKGNVRKIETHLDRVDLAIQVENQQIWSTISLWSFNEL
                     AIQPEQEVYLQIKSVSL"
     gene            15470..15904
                     /locus_tag="PXD81_000869"
     CDS             15470..15904
                     /locus_tag="PXD81_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RatA family
                     toxin"
                     /protein_id="HDL5536918.1"
                     /translation="MPIVNQSALVPYSAEQMYQLVNQYEKYPEFLSGCVSTKTLSTGE
                     NELKAELHIQKLGISQTFSTHNKMTPPYKIEMALIEGPFRHLHGAWTFTPFDEQSCKI
                     ALQLNFEFSSPIVAMVFGKVFNELTLKMVNAFKQRAKEVYGV"
     gene            15894..16229
                     /locus_tag="PXD81_000870"
     CDS             15894..16229
                     /locus_tag="PXD81_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HDL5536919.1"
                     /translation="MVSEQRQNPEEQKSEEQKINVEVLYAYPDKYFLKKLQVPQNATI
                     QTIILLSGVLEKYTEIDLRENKVGIFSRPAKLNETVEEGDRIEIYRPLIADPKEIRRK
                     RAAEQAEKK"
BASE COUNT         4622 a         3426 c         3736 g         4589 t
ORIGIN      
        1 tatcaaagga gtgagacttt ttaggattaa cttagaaaat caagctcctt tcacctttca
       61 tgctgtatag cttagtcccc acattctcca ttctaccaga gatagaaaca aaggtaacaa
      121 gatcatattt gatatggtat gtttactaga agtacctccg atttcttatt gtgaatgagc
      181 cgaaaacaag agataatata acgtttattt ttaatgagat gctttaacaa acggtcagat
      241 tttctctgtt ttttgcaaaa taaaatgcgt ttttgaccgc ttgtatattt aattttgtgg
      301 aatttatggt cgattatatt ctgttggtca tcagcaccgc ccttattaat aactttgtct
      361 tagttaaatt tctagggctt tgtccgttta tgggagtgtc gaaaaaggtg gaaactgcta
      421 ttggtatggg gttggcgaca acctttgtgc tgacggttgc ctcgctctct gcttatttga
      481 tggaaaccta tttgcttgag ccgttaaacg ctcaattttt gcgtaccttg gtgtttattt
      541 tagtgattgc ggtgatcgtg caattaacgg aaatgattgt gcataaaacc agcccgacac
      601 tttatcgctt gcttgggatc tatttaccgc tgattaccac caactgtgcc gtattagggg
      661 tggcattatt aaacgtgaat ttagccaata acttggtgga atctgttctt tatggttttg
      721 gagccgctgc cggtttctcg ctggtgttag ttttatttgc ggctctgcgt gagcgtttgg
      781 ctgccgctga tgtgcctcgt ccgttccaag gagcatcaat tgctctgatt accgcaggct
      841 tgatgtcgct tgcctttatg ggctttacag gattggtgaa actctaatgc ttgatttgcc
      901 aattatcgct tatattctta tcgctattgc tgtgatcgca ctgattttcg gggcggtttt
      961 aggctattcc tcgattaaac tgaaagttga atccgacccg attgtcgatc aaattgatgc
     1021 ccttttacca caaagccaat gcggtcaatg tggctacccg ggctgtaagc cttatgctga
     1081 ggcgattgct aatggtgatg tgatcactaa atgtgtgccg ggtggacaac ctttagtggt
     1141 gaatcttgcc gaattgcttg gggtagaagt gccgccaatg gaaggtatgg cagagcctga
     1201 gatgaaagtg gcttttgtga ttgaagatct ctgcattggc tgtactaagt gcattcaggc
     1261 ttgcccggtt gatgcgatta tcggcacgaa taaagcaatg cacaccatta ttccggatct
     1321 ttgcaccggc tgtgaacttt gtgtcgcccc ttgcccgacc aactgtattg aaatgcgacc
     1381 aattaaaccg acaacccaat cttggaactg gaaatttgac caagatttaa ttattccgat
     1441 tgtgaatacc actgaattgc agagaaaatt agttaccggc ggaggcaaaa atgagcaatc
     1501 ctaatttagt gattgagcga atccgccaaa ataaagaaac gggtaaactg tgggatttcc
     1561 cgggcggtat tcacccgatg gaaaataaat cccaatcaaa cagcaagcca attcgcacct
     1621 taaatttacc gaagtttttc tatgtgccac tggttcaaca ttctggctgg gcaggggaat
     1681 tattagtcaa agtcggcgat gtggtattga aagggcagcc gctcacgaaa ggcgatcact
     1741 tccgtcaact gcccgtacac agcccaacct ccgggcgggt gattgggatt gagccttatg
     1801 tttccgctca cgcctcaggt ttgcccgaaa taacggtgat tattgaaacc gatggtaaag
     1861 ataaatgggt ggaacgccag ccgattgagg attttttaag cctaacaagc gatcaaatta
     1921 tccaaaaagt ttaccaatat ggcattgccg gtttaggcgg tgcggtattc ccaaccgcaa
     1981 caaaattaag tttggcggat aaacgctgta aattactggt gattaacggg gcagaatgtg
     2041 agccttacat cacctgtgac gatcgcttaa tgcaagattt cactgcggat cttatcgaag
     2101 gtattcgcat tctgcgttat gtgcttcgcc cggaagaagt ggtgattgcg attgaagaca
     2161 acaaaccgca cgctattcaa gctttagaaa ccgctttaaa aggctcgaac gatatgtttg
     2221 tgcgggcgat cccgactaaa tatccctccg gagcttctga tcagctgatt caagtgttga
     2281 ccggacttga gattccggct ggcaaacgca ctattgaaat gggcattgtg atgcacaatg
     2341 tcggcacggc gtttgcggtt aaacgggcaa ttattgatga tgaacctttg attgaacggg
     2401 tagtgacgct gacaggcgat aaaattcgca acaaggggaa cgtgtgggca agattaggca
     2461 cgccaattca gcacttgttg gagcaggtag attatcaagc cgataaacgc tttccggtgt
     2521 ttttaggcgg accgatgatg ggctttattt tgcctgcatt gcaagccccg attaccaaaa
     2581 ctgcgaactg cttgattgcc cctgatcatt ttgaatatgc accacctgaa ccggagcgta
     2641 gttgtatccg ctgttcaagc tgttccgatg cttgcccggt ggggttattg ccacagcaac
     2701 tttattggtt tgcccgtgca gaagatcacg ataaatcgaa agaataccat ttagatgcct
     2761 gcattgagtg tggcgtttgt gcttacgttt gcccaagcta cattccgtta atccaatatt
     2821 tccgccaaga aaaagcaaaa atcgaggaaa ttgaggaaaa agcccgcaaa gcggaagagg
     2881 ctaaaatccg ctttgaggcg agagaagctc gcttacaaaa agaacgagat gccagaactg
     2941 ctcgcattag ccaagcggcg gaaaaacgcc gtgaagaagt ggcaaatgca ggtggtgttg
     3001 atcctgtaaa agcggctctt gaacgcctga aagcgaaaaa agcggttgaa acagtccaag
     3061 aaataccggc agaagtacag gtaaaaccta acggcgagcc tgataacagc gatttaatgg
     3121 cacaacgcag agccagacgt ttagccaaac aggcagaaca agcggtcgaa aaatcggaaa
     3181 atgttacaaa tgccttgatt ggggaaaata atgatttgcc tctacaatca atgaacgaaa
     3241 ctgcatcgga taaatctgta ggggcgaata actattcgcc cgaagcagaa agcgaagacc
     3301 cacgcaaagc cgccgttgcg gctgcccttg cccgagccaa agccaaaaaa caggcacagc
     3361 aagcggtcga aaaatcagaa aatattacca atgccgtgat tggggtaaat aatgatttgc
     3421 ccctacaatc aatgaacgaa acggtatcgg ataaatctgt aggggcgaat aaccattcgc
     3481 ccgaaacaga aagcgaagac ccacgcaaag ccgccgttgc agcagctatc gctagagcta
     3541 aagcgaaaaa acaagctcaa caagcggtcg aaaaaccgga agatattgcc aatgctgtga
     3601 ttggggtaaa taatgatttg cccctacaat caatgaacga aactacatcg gataaatctg
     3661 taggggcgaa taaccattcg cccaaaacgg aaagcgaaga cccacgcaaa gccgccgttg
     3721 cggccgccat cgccagagct aaagcgaaaa aagccgctca gcaggagaaa agcaacaatg
     3781 tttagcgagc taattccggc attgccgtgg tcgttcctgt ttattcagga cttaatcggc
     3841 actatcgtgt ttgcgatttc gggggcaatt gctgcccgcc agcacaaaat ggacattttc
     3901 ggtatgttta ttttagcgtt tgtgaccggg gtcgggggcg gtacgctaag ggatatgatg
     3961 attggctcca cgccggtgtt ttggatgaaa caaccgatct atgtgctgat gattaccctt
     4021 gcagtggtga ttaccgctat tttccgcaac caaatttccc gcaaaagttg gcaaaccggc
     4081 ttgctggtgt ttgatgccat cgggcttggc gtatttaccg tgattggcgt gcaaaaaggc
     4141 ttggatttcg gcttgcaccc actgatctgt gtggctctgg gcggtgtgac aggctgtttt
     4201 ggtggcttaa tccgtgatat tttacgcaac gaagtgccga tcattttgca aaaagaagtg
     4261 tatgtgaccg ccagccttat cggtggtgtg atttttgtgg ctgcaaactc agttgggctc
     4321 gaaagcgatt gggtttacct tgccaccgtg ttcaccgtaa ttattatccg cctgctggca
     4381 atacgcttta atttgaattt gccgagactt taataagcgg tcattttttt gcaatttttt
     4441 actaaaaagg ttttatagat gtttaaaatg gtcagttccc cccacactca ttcgagcaat
     4501 ctcactgcga attttatgct gtgggtgatt gcgatgatgt tacctgcttt tggggttcag
     4561 ctttattatt tcggctatgg tgtgctgatt caaacgttgc ttgccgttag ccttgcgatt
     4621 gtgattgaaa tcgggattgc aaaattacgc aaaaaaacaa ccgctttcta tttggctgac
     4681 ttatcaggta ttttaaccgc cttgattttg gcgatgtcga ttccgcctta tgctccttat
     4741 tggcttgtgt tgattggcgt gattgtggcg ttattattgg caaaacacag ttatggtggc
     4801 ttggggcaga atctatttaa cccggcaatg gttggctatg cgttgttgtt ggtttctttc
     4861 ccggtgcaaa tgacggcgtg gatagtgccg attgagttat taagtgaacc accgacattt
     4921 cgtgatgcga tttcgttgat tttttacggg gtaactagcg atggttttag tattcatcag
     4981 ctagttaata gtgttgatgg cattgcacaa gctaccccgc ttgatagtat gaaaagcagc
     5041 cttgcaaaat caggttttga cgcggtaatg aatacgccga ttttcaacgg gctgtttgcg
     5101 accggttggc tacaactgaa ccttgcgttc ttattcggcg gtttggtgtt gatttataaa
     5161 cggattatcc attggcaaat tccggtagca atgttggcaa cttttgcctt ttttagtgga
     5221 ttcaccgagc tgtttagcca acaatcgcac ttgcattttg tgagccattt ctttagcggt
     5281 gcgatgatgt tcggggcatt ttttattgcg accgacccgg taacagcctc cattacccca
     5341 agaggaaaac tgattttcgg tggcttggtg ggattattgg tttatctaat ccgctactac
     5401 ggaaattacc ctgatgcgat tgcattttca gtgttattgg caaacatttg cgtaccgtta
     5461 atcgaccatt atacgcagcc gagattatac ggcacaaaat tggataacag ataatgaaca
     5521 cctcaaaaat taccttaaat tacgccttaa ttttaggctt tattgccttg ctttgcaccg
     5581 ctgtttcaac cggtgtgtac ctgcttacca aaggcaagat tgatgatgta accgcaatgc
     5641 aacaacgcaa attattggaa gaggttgtgc cgaaaagcca ttttgataat gatgtgttgg
     5701 cgacttgcaa actggttcag ctcgaaaatg ccccttattt gaaccgtatt tatgttgcaa
     5761 aaaaatcgga aaatctgacc gcttacctag ttcaaggaac ggctccggac ggctattccg
     5821 gcgatattgt gctattgatg gggattgagc cgaacggcaa cattttaggc gttcgcaccc
     5881 ttgagcataa agaaactccg gggctgggag ataaaattga gacccgagtg tcggattgga
     5941 tcttatcttt taccaatcaa ctttttagtt tagaaaacga agcggattgg aatgtaaaaa
     6001 aagatggcgg caagttcgac caatttaccg gtgcaactat tacacctcgt gcggtggtca
     6061 atcatatccg ccaaagtgcg aaatgggtgg tgacagagct ggctcaaaac ccgacaatgt
     6121 ttaatcaatt taaagcgtgc aaataggggg agaaatggaa agagaacaac aaatccctgt
     6181 gacgaatatt gacgttgccc cgaaacaatc cagcgtttgg aaaacgattt tcgctgatgg
     6241 tgtgtggaaa aataatggtg ctttggtgca attattaggg ctttgcccac tactggcggt
     6301 ttcaaacagc gtaaccaatg cacttggact aggcttggca acaatgtttg tgctaatttg
     6361 caccaatatc acggtttccc tgtttcggaa aatcactcca cacgatattc ggattccgat
     6421 ttatgtgatg gtaattgcaa cggtggtaac ggcagtacag cttttaatga atgcttttgc
     6481 tttccctgtt taccaatcgc tcgggatttt tattccgctg attgtaacca actgtatcgt
     6541 gattggacga gcagaagcct atgcctcaaa aaatgaggtc aaattctcgg cttttgacgg
     6601 cttttcaatg gggctgggaa tgacttttag ccttgtggtg ctaggcgcgt tgcgtgaaat
     6661 tattggcaac ggcacgctgt tttcaggctt agatctactc cttggcgatt gggcaaaagc
     6721 cctacgcatt gatgtattgc atattgattc aggtttacta ctggcaattt tacctccggg
     6781 ggcttttatt ggacttgggg tgatattggc ggtgaagaat attattgata agagatgatg
     6841 tgttatgtca cttttctttg cttgtgcaaa gaaaagtaac caaaagaaag cacaccctgc
     6901 tttgttgcta tcctcacttg gttgaaattt tcttaacgaa aaataaccct gatgttcgct
     6961 tcgctctcgc tcgagttatt ttcctaaaaa tttcaagtcg ttcgggcaac tcagaaggga
     7021 acccgatccg accatacaag cggtgatttt tttggaaaat tttgcaaaat gatgctctgc
     7081 cgggttccct tctgaaccgc ccgaacgact ttgaatttgt aggaaaaatt ccttgtttga
     7141 gcagcgaagc ggcgagtttg gaattttttc gttaagcaaa ttcaaaccaa gcgaagatag
     7201 cggtgaagta gggttgcctt tctttggtta ctttctttgg cgaagcaaag aaagtgacaa
     7261 ataatgagga attatagaat gacacgaatt accgcccaag acggctatac gcacaaattc
     7321 ctatggtctt ttctacaccc gaaatattgg ggcgtatggt taggtgtatt aggattagtg
     7381 attttagcct atgtgccggt gcgaattagg gataaattat ccgcttttgt gggtaagatg
     7441 gcgtatcgct atctcaagca gaaaaataaa aaaggctacc accgtgctaa agtgaatttg
     7501 cgttactgtt ttcccgattg gagtgagaaa aagcgtgaag aagtggtcga gaaaatgttt
     7561 atcacggtag cacaaaccat gttggcgatt ggcgaaactg caatccgccc ggcctcttat
     7621 ttacaaaaac ggtgtgaatt taccggcttt gaaaatgtgg tgaaagccaa agagagcggt
     7681 aaaaacgtga ttatgttagt gccacatact tggtcgattg atatggcggg ggtggcgatg
     7741 ttctcgctcg gttatccgat gacatcaatg tacaatccac accgtaatgc gttggtggac
     7801 tggctgtgga atattacccg tgagcgttta ggtgggcgaa tgcacactcg tcaaaatggg
     7861 attaaagcgt ttttagccga tgtgcgtaaa ggtgatgttg gttattttct gcccgatgaa
     7921 gatttcggcg aagagctaag tgtgtatgcc gacttttttg ccaccgaaaa agccactttg
     7981 ccgggcttaa ataaaatggc aagagtggca aatgcggaag tgatcccaat gttctcgatt
     8041 tataatgcgg aaaaagcaaa atatgaaatg gaaattttgc caccgcttga attttcagga
     8101 gacccaattc aatccgcccg agaaatgaac cgagtaattg aatattttgt ggagaaaaac
     8161 cccgagcagt atgtttggat tttacgtttg cttaaaacca gacgaaacag ggaagatatt
     8221 tatctataat atagggaaaa ggaaactgaa aagtaagctg tactatcaaa cgtaaggtta
     8281 tcaatttctc cttgaaattt ctgctgtttt tctatagagt tttccagtta tttttttatt
     8341 acacgtaggt caaacaatga accaattaga tttaattaaa tcttctatca aatccattcc
     8401 agattatccg aaagcaggga ttatcttccg tgatatcact tcacttttag aagtgccaac
     8461 tgcattccaa gccaccattg atgccatcgt tgcagaattt aaagacaaag gtattactaa
     8521 aattgtcggc acagaatctc gcgggtttat ttttggtgcc cctgttgcat tagcgttagg
     8581 cgtgcctttt gtgttggttc gtaaaccgaa aaaattacca cgtgaagtga tttcgcaatc
     8641 ttacacgctt gaatatggtg aagacacgct agaaattcat aaagacgcag tgagtgctaa
     8701 tgataatgta ttggtcatcg acgatttact cgcaaccggt ggtactattg atgcaactgc
     8761 aaaactaatt cgtcgcttag gtggtaacgt agaacacgct gcctttgtga tttggctgcc
     8821 tgagttaggt ggtaaagagc gtttggaaaa agaaggggtt aaatccttta gcctagtcag
     8881 ctttgaaggt cattaatggg gaacaagcgg tggctttttg ctaaaatttt gcaaaatttt
     8941 tcggaaagat gaccgcttgt gatttccaaa ttgaatagaa aaatcaatga gttatcaagt
     9001 tttagcccgt aaatggagac cgcaacgctt ctcggaagta gttggtcagc aacacgtttt
     9061 atctgctctt gaaaacagct taagagaaga tagactgcac cacgcttacc tgttttcagg
     9121 cactcgtggt gtgggtaaaa cctctattgc ccgtttattc gccaaaggct taaactgtgt
     9181 aaacgggatc accgccgagc cttgcggtga atgtgcgaat tgtaaagcga ttgaagaagg
     9241 gcattttatc gatctcattg aaattgatgc tgcttctcgt actaaagtgg aagacacccg
     9301 tgagctactt gataacgttc aatacaaacc caccgttggg cgttttaagg tttatttaat
     9361 tgacgaagtg catatgctct ctcgtcatag tttcaacgcc ttactaaaaa cccttgaaga
     9421 gccgcctgaa tatgtcaaat tcttgctggc taccactgat ccgcaaaaac tgccgattac
     9481 gatcctctct cgctgtatgc aatttcatct gcgggcatta gagcaatcgc aaattgcccg
     9541 tcatcttgaa tttattctaa atgcagagaa cattccgttt gagcctttgg cactagaaaa
     9601 gttggcaaaa gcagcacaag gtagtatccg agactctctc agcctcaccg atcaagccat
     9661 tgcaatgagt aatgccaata ttacgcttgc gatagtcagc caaatgctag ggttgattga
     9721 cgataatcaa ccactagaat tagtgcaagc aaccgcccaa gccgacggtg aaaaagcaat
     9781 ggcgatcatc gagcaaattg cagcgaaagg cgttgattgg caacaattat taagcgatgt
     9841 ggcagaagtg ttacacaaaa ttgcaatgtt gcaattagtg aaaacaccta caaccgaaga
     9901 aacattagtg cattttctgg caaaacaaat tccgcccgaa gacgtgcaat ttttctatca
     9961 attaatcctc aacagtaaaa aagagttgcc ttttgcaccg gagcaacgtg ccggtgttga
    10021 aatggcgatt ttgcgtgcgt tggctttcca ccctaaacag gcagaaaaaa tagcaacagt
    10081 tgcagtggaa aaggttacac aaccaaacca ttcggtttca gcggttacga cagcaaaacc
    10141 acaaaatcaa attagccagc taagagaaaa ttttgagcaa aatcagcaaa aaacggttac
    10201 tgttgccaat actaccacag cggttgaaag cattgctcaa ccgccacagg cagtagatac
    10261 cgctatttcg ccgtctgctc ctgcgactct ttcaccggca atggcagccc tcgaagcccg
    10321 caaaaagcta caagcacagc aggcaaattt agctcaaacc gaaaaaaaaa agcctgagct
    10381 aaaagcccct caagttattg caaaaaaatc ggcaaatcag accgcttcat tgcaagagcg
    10441 gtttatcagc ttggcggaaa atcagcacaa agccgtactg caagtttcaa agaaaaccga
    10501 atccgcccct aaaagtgtgg aagatgaaga ttatcgctgg acgtggctca atcctgaatt
    10561 agaaaatcaa gcagacagtg ccaaaccttc tgatattaaa caagcgattt tgcaagaacg
    10621 cacccctgaa ttagtggcaa aaaccattca tttatcttgt cagcaggata aatggtgtga
    10681 catcgccaac tcactcaatt taggcggatt ttcccgtcag atcgccttaa atagctattt
    10741 agcggaacaa aatgaacagc acctcgcatt ggtgttaaag ccgaatatgg ctcacttaga
    10801 taatccggaa tcacgtaaaa atttagaagc tgcccttgcc gaaaaaggct attcatacga
    10861 attgaccatt ggtgacaata gcgaacagca aactccgctt gaaattcggc gagccatttt
    10921 caaacaatta actgaggcag caaaatcagc tctgcttaac gatgaaaaat taatgttact
    10981 tcgcaacgct tttgatgcac aaattgatga atcgaccatt cgagcggtgg cggaaactga
    11041 gtaaaacaaa tgaaagcatt agaattaatt gatttggtaa aaacctaccc aacaggtgta
    11101 aaagccgtta aaggcattaa tcttgtggtc gagcaaggtg atttctatgc cctacttggg
    11161 cataacgggg caggtaaatc tactactatt ggcattatca gctcactggt aaataaaacc
    11221 agcggcagcg tgaaagtgtt tggctatgat ttagacaccc aaaaagtcga attaaagcat
    11281 caaatcggct tagtgccaca agagtttaat ttcaatcagt ttgaaaaagt gattgatgtg
    11341 ctagtgcaac aagccgggtt ttacggcatt cctcgcagtg aagccttagt acgtgccgaa
    11401 aagtggctga aattgctcga cctatgggaa aaacgcaccc acctcacccg tgagctttct
    11461 ggtggaatga aacgtcgggt gatgattgcc cgagccttaa tgcacaatcc aaaactactg
    11521 attttagatg aacctaccgc aggggtggat attgaactcc gccgctcatt gtgggatttc
    11581 ttgcgggatc taaacaatca aggcacaact attattctca ctacccacta tttagaagag
    11641 gcggaaaccc tatgccgtca catcggcatt attcaacacg gcgaattaat tgaaaacacc
    11701 tcaatgaaag ccttgcttgc caaattggaa agtgaggtat ttgtgttaga tttgcaaaat
    11761 tctgagcaaa atcaaccgct tgtgattcaa aattacccgc ataaatggct tgatgaaaac
    11821 acccttgaag tgaatgtaaa acgccagcaa gggctaacca atctgttttc gcaaatttcc
    11881 gcccaagggg ctgaggtgct aagtatgcga aacaaatcaa accgcttgga agaactattt
    11941 atcaatatgg ctcagtaaaa tgaatttaat cggattttat accttagctt acaaagagtg
    12001 ccgccgcatt ttgcggattt ggcgacaaac tttagtgcca ccggtgatta ctaccacgct
    12061 ctatttttta atcttcggca cgctgattgg tgggcgaatc ggggaaatga atggcgtggg
    12121 ttacacccaa tttattgcac ccggcttaat tatgatgacc gccatcaccg cctcttatgt
    12181 gaataccgcc tcttcatttt tcttgagtaa atttacccga gattttgaag agatgctgat
    12241 ttcgccactc tctacccata atattatttg gggttatgtc accggcagta ttttgcgtgc
    12301 aggtttagcc gcaaccttag taatgtgcgt ggcaatggtg tttgtctcgt ttgaaatcta
    12361 ctcggttgcg attatcgtct taaccctgtt gctgacaatc attgcctttg cgttaggtgg
    12421 cgtaattaat gcggtgttcg cccgctcctt tgatgatgtg gggctgattc caacctttgt
    12481 gcttactccc ttaacttact taggcggcgt attttactct atttcattac tgcctgaatt
    12541 ttggcaaacg gtgtctaaat tcaacccgat cgtttatatg attaacggct taagatatgg
    12601 ctttctcggt atcagcgatg tgccgttagg ttatactttt gcagtgctta cttcactttg
    12661 catcggcttg tatattattg cctattcctt aattcaacga ggggttgggc tgcgttctta
    12721 actaattgtg aggttatgaa aatgaaagca ttaaaaaaac tagcaattac aatggcatta
    12781 acgttatcag gcacagcctt ttcacaagag attaccgtgt ttgctgccgc ttcaatgaca
    12841 aatgtgttac aagatattca gcaggatttt gaaaaagcct atccacagga taaaatcacg
    12901 ttctccttcg cttcatcatc ggtattagca aagcagattg agcaagatgc accggcagat
    12961 atttttattt ccgccgacca aaagtggatg gattatgttg ccgaaaaaca acctgagaaa
    13021 gttaaacatc gccgcaattt agtcagaaac gagttagtat taattgcacc taccgaaagt
    13081 aacattgagc caaaagcgat acaagcggtc gattttgcca aagaattagc aaacagctat
    13141 ttatcggtgg gagacccaaa ccacgttcct gccggcaaat atgccaaaaa agcccttagt
    13201 cattaccaat tatgggaaag tgttgaacct cgtttagcga gggcaaaaaa tgtacgagat
    13261 gccctctctt ttgtcgagcg aggcgaatcc ccacttggga ttgtctattc caccgatgct
    13321 aaagtgtcga ataaagtcaa agtggttgcc gttttcccac cggaaagcta cggcgaaatt
    13381 gtttaccctg ctgcggcagt cagtgaaaaa gcctctgccg ccacattttt agattttctc
    13441 aaaaccccga aagcaaaagc gaaatttgag gcagctgggt tttatcctgt aaattaaatt
    13501 tactctctcc ccttgtggga gagagacaga aaaatatgtc gttcagacat atttttcaga
    13561 gagagggcaa atagcgttta aaatattccc cctctccctg atttttcttc tgaacgaaga
    13621 aaaatctatc cctctcccac aaggggcgag ggtaagctta taaccaaata gttttagatt
    13681 atgcttaatt tcaccccaca agaactcaat gcgattgttc taagcctaaa agttgcctcc
    13741 attgctgtag tgcttgcatt accgcttgcc gtgtgggtgg cgtggctgct tgcccgcaaa
    13801 cagttttggg gaaaaaatct gctcaatggt attatgcatc tccctctggt gttaccgccg
    13861 gttgtcattg gctatttatt gcttatttcg atggcgaaac gaggggtaat cgggcaatat
    13921 ttgtgggaat ggtttcattt cagcttgagt ttttcgtgga aaggagcagt gcttgcctca
    13981 atggtgatgg cgtttccgct aatggtgcgt tctatccgtt tagcctttga ggcagttgat
    14041 cctaaattgg agcaagccgc cagaacgctt ggagcaatac cgttaaaagt gtttttcacg
    14101 ctcactttac cgctctcttt ttcaggcatt attgccggtg gcgtgctggg ttttgcccgc
    14161 tctttggggg agtttggggc aaccatcacc tttgtctcca acattcccaa ccaaacccaa
    14221 accattcctg ccgccctttt cacctttatt gaaacccccg atggcgaaat ggctgccgcc
    14281 cgtttgtgct tggtggcgat tctgatttca ctcattgccc tgttctgttc ggaatggctg
    14341 gcagaaaggc aaaaacgcta tgctcaaact taaacttctt cagcaactcg gcaatctgac
    14401 gctcaatgtc aatctcgatt tgccgaataa aggaatcacc gctattttcg ggcgttccgg
    14461 tgccggtaaa tccagcctga ttaatttaat tgcggggctt tcaaccgctc aagccggctt
    14521 tatcgaatta aacggtcagg tgctgtttga cagccaaaac ggcatcaatt taccacctga
    14581 aaaacgcaac attggctatg tgtttcaaga gcatcgcctt ttccctcatt attcggtgga
    14641 aaaaaactta aaatatggct acaagcggtc ggattctgcg gattttttgc aaattgtgga
    14701 actgttaggc attgcccctt tgcttaatcg ctatccggca agcctttccg gtggcgaaaa
    14761 acaacgtgtg gcgattgggc gggcgttatt aagctcaccg aatattttgc tgatggacga
    14821 accattatct gccctcgatc tccctcgtaa gcaagagcta ctggactatt tagataaatt
    14881 atccgctcgg cttgaaatac cgattctgta tgtcagccac agtttagagg aagtagttcg
    14941 attggcagat catcttgtgc tgttggaaaa cggcaaagta accgcatttg ataccctttc
    15001 agcggtttgg cacagctcgg cattttccgc ttggcagcca aatcatcagc gaattagcct
    15061 gcttgaatta ccggtggaag aaacacttcc ccactataat atgcttggct tgagcttgaa
    15121 cgcacagcga ttatgggtga accaactccc ccgccatcaa ataggcgaga agctaagggt
    15181 aactattgcc agcaaagagg tttcaatcag cttacaaaaa ccgactcaaa gctcaattcg
    15241 taatgtgtta aaaggcaatg ttcggaaaat agaaacgcat ttagatcggg ttgatctggc
    15301 aattcaagtc gaaaatcagc aaatttggag tacgatcagc ctttggtctt ttaatgaatt
    15361 agcgattcaa ccggagcaag aggtctatct tcaaatcaaa agcgtttctt tataggcttg
    15421 caaatttacg ctaaaatatg accgcttgtt aattcccaaa agagacatta tgccaatcgt
    15481 taatcaatct gccctcgtgc cttatagtgc cgagcaaatg tatcaacttg ttaatcagta
    15541 tgaaaaatac cccgaatttt tatccggctg cgtgagtacc aaaaccctta gcaccggcga
    15601 aaatgaatta aaggcagagt tacacattca gaaattaggc attagccaaa ctttcagtac
    15661 ccacaataaa atgacgccgc cctataaaat cgagatggca ttaattgaag ggcctttccg
    15721 ccatttacac ggagcttgga cattcacccc ctttgatgag caaagttgca aaattgccct
    15781 gcaattaaat tttgaatttt ccagcccaat tgtagcaatg gtgttcggca aggtgttcaa
    15841 cgaattaact ttaaaaatgg taaatgcttt caaacaacgt gcaaaagagg tgtatggtgt
    15901 ctgaacaacg tcaaaatcca gaagaacaaa aaagtgaaga acagaaaatt aatgttgagg
    15961 tgctttatgc ttaccctgat aaatattttc tgaaaaaact gcaagtaccg caaaacgcga
    16021 ccattcaaac tattattttg ctctccggcg tgttggaaaa atataccgaa attgacttgc
    16081 gtgaaaacaa agtcggtatt tttagccgcc cggcaaaact gaatgaaacg gtggaagaag
    16141 gcgacagaat tgagatctat cgcccgttaa ttgctgatcc gaaagaaatc agacgcaaaa
    16201 gagcggctga gcaagcggag aagaaatagc tttctcctat tcaatctagg ggtgaataat
    16261 tattcgccct atctattttc tccctcgcca ttcctgctat aaattccctc ctttgattaa
    16321 atatgaaaat ctttcataaa ttttatgtaa attttgagtt tgattatatt taa
//
LOCUS       DANLYI010000037        16278 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.037, whole genome shotgun sequence.
ACCESSION   DANLYI010000037 DANLYI010000000
VERSION     DANLYI010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 16278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16278
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.037"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            <1..1571
                     /locus_tag="PXD81_000871"
     CDS             <1..1571
                     /locus_tag="PXD81_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005606677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cell division protein FtsI"
                     /protein_id="HDL5536920.1"
                     /translation="KSEGYWTQLAQVTGLDYNSLIEKVRNNPSSVFLRLDNENLVRER
                     QKFQALSKEIGRSYNDIMDELYSKNRQRFLYLSRHQSEAISEYAKELDIDALVIKVES
                     RRFYPLAEESSQLIGFTDKDDMHGSEGLERSFDSLLIGKNGKQIIRKDAKGNIVENIR
                     SEKQYDPQDVMLSIDEELQSMVYGEIKKAVQENNAESGTAVLVDVQTGEILAMANAPS
                     FNPNKRDSFKPELMRNRAITDTFEPGSTVKPFTVLTALQNGVTYRDEVIGTRPFVVNG
                     HTIKDVAPRDSLTLTGILQKSSNIGVSKLALRMPANALVDTYTKVGFGKDTGLGLGEQ
                     RGTNGDRKRWADIERATLSYGYGLNVTPLQLARAYATLGSFGLYRPLSITKVDPPVIG
                     ERVLPEKTTRDVVHMMESVAAKGEGGHRAAVDGYRVAIKTGTARKLEKGKYVEKYIAY
                     TAGLAPASDPRFALVVLINEPKAGNYYGGFVSAPLFSRIMGYTLKQRNIKPDNLTENT
                     QSAVREIKLERPIN"
     gene            1580..3070
                     /gene="murE"
                     /locus_tag="PXD81_000872"
     CDS             1580..3070
                     /gene="murE"
                     /locus_tag="PXD81_000872"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HDL5536921.1"
                     /translation="MKRLLPFLTELDAWVEELTELKQMTLDSRQVQAGDLFVALKGHQ
                     VDGRKFIPNAIEQGAAIILAEADEDQPEIELDPKFAKFNLDRTACCKVVSVPRLPKLL
                     SEIAGALYHNPSHKLTLAGITGTNGKTTTAQLLAQWHNLLGGKSAVMGTIGNGLYGQV
                     QEAVNTTGSAIEIQHNLANFVELGADFCAMEVSSHGLAQFRAEALDFDLAIFTNLSRD
                     HLDYHNTMEEYAQAKFRLFNELSTKAQVINADDEIGREWLTQLPNAVAVSTDPKFAGN
                     HQFVKATAVKFTLQGASIAFESSWGNGELHSRLIGAFNVNNLLTAFAGLLALGFDIQE
                     LVKTAPNLVGVAGRMECITAPNKPMVIVDYAHTPDALEKALQAARLHCEGELWCIFGC
                     GGDRDAGKRPLMATIAEKLTDKVIATDDNPRTEDNQKIMADIVKGFSKPQQIIHNREE
                     AIKTAIEQAKATDVILIAGKGHEDYQIIGTEKLHFSDQETARKYLF"
     gene            3158..4552
                     /gene="murF"
                     /locus_tag="PXD81_000873"
     CDS             3158..4552
                     /gene="murF"
                     /locus_tag="PXD81_000873"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HDL5536922.1"
                     /translation="MIKLTTQQIAEILNAKLIGNGETVVETTSTDTRQAVENGLFFAL
                     KGENFDAHHYLQNAVAQGCVAVVVERECEIDVPQLVVADTRLALGELAKWLKAKLNPK
                     TVAMTGSSGKTTVKEMTAKILQKMTACEQEVLYTFGNLNNDLGVPMTLLRLTEQHKYA
                     VIELGANHIGEIAYTTAIAQPDACLVNNVAAAHLEGFGSLAGVAQAKGEIYRGLKENG
                     KAIVNLAFYYPQWQKEIGSREMQSFSYIGSDSDSYADYWAEDVELHLSGSRFTLHTPQ
                     GEIAIDLPYLGKHNVSNALAATSLAMSVGATLEAVKAGLEQRSMVKGRLYPVQINDHC
                     LFIDDTYNANVDSMKSAISVLENYPAYRIFAVGDMAELGEESSKCHQEVADFAKNAAL
                     DLIVSFGKESAVISQQAEHHFTDKQQMVDFLLPIILQKIEQKQPLVLLAKGSRSQKME
                     TVISELCKPFNLNF"
     gene            4573..5655
                     /gene="mraY"
                     /locus_tag="PXD81_000874"
     CDS             4573..5655
                     /gene="mraY"
                     /locus_tag="PXD81_000874"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HDL5536923.1"
                     /translation="MLVWLAELLVQYNTAFNVVSYITFRAIMALLTAMGIGLWIGPKV
                     IRRLQILKFGQEVRNDGPESHFKKRGTPTMGGVMILAAIGVSTLLWADLRNSYVWFTL
                     FVLFGYGAVGFVDDYRKISRKNTDGLIARWKYFWLSAIALVAVFGMYAVGKDTAATQL
                     VVPFFKDVMPQLGLFYIVLSYFVIVGTSNAVNLTDGLDGLAIVPTIMVASAFALIAWA
                     TGNFNFAQYLHIPFIPNAGELVILCTAIVGAGLGFLWYNTYPAQVFMGDVGSLSLGGA
                     LGVIAVLVRQELLLVVMGGVFVVEALSVILQVGSYKLRQKRIFRMAPIHHHFELKGWS
                     EPRVIVRFWIITLMLVLVGLVTLKLR"
     gene            5719..7020
                     /gene="murD"
                     /locus_tag="PXD81_000875"
     CDS             5719..7020
                     /gene="murD"
                     /locus_tag="PXD81_000875"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005606684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HDL5536924.1"
                     /translation="MQKQYENKTVTIIGLGTTGLSCVDFFKKKNANIQVIDTRANPAG
                     ADKLDVTIPLHTGGLNSEWLLKSDLIVISPGLAVATPEIQQAIQAGVEIVGDVELFCR
                     EAKAPIIAITGSNGKSTVTTLTTEMAKQAGIKVGMGGNIGVPVLSLLEGDYDLFVLEL
                     SSFQLETTYSLKAKAATVLNISEDHMDRYDSIADYRQAKLRIYDNAENIIVNGEDAQT
                     YPIQAVKKVIRFAEQNAEYSLRNQMLYSGDDAIIQTKQMLISGRHNEMNALAAMALAE
                     AANVPREGIVKALQIYGGLPHRFQAVATNDGVRWVNDSKATNVGSTVAALNGLNIAGT
                     LYLLLGGDGKAADFSELKPLINKPNIVCYCFGQDGAALAELSEQSVLVETMQQAIEAI
                     RPQLKKGDMVLLSPACASLDQFKNFEQRGEVFMALAQGQHA"
     gene            7020..8198
                     /gene="ftsW"
                     /locus_tag="PXD81_000876"
     CDS             7020..8198
                     /gene="ftsW"
                     /locus_tag="PXD81_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HDL5536925.1"
                     /translation="MLERIKSEWDKWVRLTPSNSLYDRTLIWLFLGLLIIGFVMVTSA
                     SLPVSTRLNNDPFYFAIRDGLYIIASIIFCYVFVQIPIEKWEKHNLALFFISIGFLIA
                     VLIFGRSINGAVRWIPLGILNFQPAELAKLAVICYFASFYVRKYDEIRKEKASFWRPA
                     VILFLFGFLLILQPDLGSTFVLFVLTFSMLFIVGAKIMQFMFLGVVGTVLFAVLILTS
                     EYRLKRVTSFMDPFADAYGDGFQLSNSQMAFGQGEFWGQGLGNSVQKLEYLPEAHTDF
                     VMAVIGEEFGFFGIACIVLLLILLTVRALKISKESLVLEERFKGYMAFGIAIWVFLQG
                     FVNLGVASGLLPTKGLTFPLVSYGGSSLVIMSIAIAVLLRIDHENRAERIGHAKIKTI
                     "
     gene            8317..9372
                     /gene="murG"
                     /locus_tag="PXD81_000877"
     CDS             8317..9372
                     /gene="murG"
                     /locus_tag="PXD81_000877"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HDL5536926.1"
                     /translation="MAKKLLVMAGGTGGHVFPAIAVARELQKQGWQIRWLGTKDRMEA
                     TLVPKHGIEIDFIEISGLRGKGVAALLKAPFAIFKAVMQARKIIKNYQPDAVLGMGGY
                     VSGPGGIAAKLCGVPVILHEQNAVAGLTNVWLSKIARKVLQAFPTAFKEAEVVGNPVR
                     EDLSALPLPNERFAKRGYPINILVMGGSQGARVINQTVPEVAKVLGNNVFISHQVGKG
                     NLAGIEEVYQQTGNGIASEFIDDMKAAYEWADLVICRSGALTVCEIAAVGLPAVFVPF
                     QHKDRQQFLNANYLAADGAAVIIEQSDFTPKALQQALEPLIANRQLLLDMSMRARAKS
                     TPMAAKRVAEVIIQEAN"
     gene            9448..10875
                     /gene="murC"
                     /locus_tag="PXD81_000878"
     CDS             9448..10875
                     /gene="murC"
                     /locus_tag="PXD81_000878"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013527829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HDL5536927.1"
                     /translation="MKNFQDKVKKLVPEMRRVSQIHFIGIGGAGMSGIAEVLLNEGYQ
                     ISGSDIADRAVTQRLSAAGAKVFIGHQAENIAGASVVVVSTAIDETNPEIIAAREARI
                     PVIRRAEMLAEIMRFRHGIAIAGTHGKTTTTAMISMIYTEAKLDPTFVNGGLVKSTGK
                     NAHLGASRYLIAEADESDASFLHLQPMVSVVTNIEPDHMDTYGGDFEKMKETYVRFLR
                     NLPFYGLAVMCADDETVMEIAPRVGRQVITYGFSENADYRIEDYQQTGFQEHYTVVCP
                     SGERIDVLLNVPGMHNALNATAALAVAKEEGIGNEAILAALADFQGAGRRFDQLGSFI
                     RPNGKIMLVDDYGHHPTEVDVTIQAARSGWEGKRVVMIFQPHRYSRTRDLFDEFVQVL
                     SKVDALILLDVYAAGEAPIVGADSKALARSIRNLGKVDPILVSDTDQLGEVLDQIIQD
                     GDLILAQGAGSVSRISRGLAESWKN"
     gene            10965..11876
                     /locus_tag="PXD81_000879"
     CDS             10965..11876
                     /locus_tag="PXD81_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014992116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HDL5536928.1"
                     /translation="MSIKNEKIAVLFGGVSQEREVSLNSGAAVTAALKSLGYNVEGID
                     TKEFPIEKLKEKGIQRVFNILHGGIGENGVLQGALEQMGIPYTGCGVMASAVTLDKFR
                     TKLLWNAVGLPTADMVVVQRGQAVDFDQIIAKLGLPVFVKSSCEGSSVGVFKVKTKEE
                     LAPAIHEALKFDSIVLVEEFLAGNEYSVPVLDGQVLPAVQVIPDGEFYDYHAKYVSDN
                     TQYVVPALNDERQAEVAKLVKAAYDVVGCRGWSRIDVMEDGEGKFRLVEVNTNPGMTS
                     HSIFPKSAATVGISFEKLVERVLELSA"
     gene            11876..12667
                     /locus_tag="PXD81_000880"
     CDS             11876..12667
                     /locus_tag="PXD81_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HDL5536929.1"
                     /translation="MGIFRKKNSTNVRLNVLNPKTPVSPRKWLVFIKPLIVLLCTLFV
                     FGVYSNWQNILESWDKTPIRAYALTHKTQFTTNADIRETLSKEPALKGYFGQNIQEVK
                     DKLLSIPWVKDVAVRKLYPDRLSITLLEHKPVAIWNDTNFVSEQGTVFSLPKDRIDKN
                     GLPLLYGPDTEGKSVLEAWNKIRADLNARNLDLTSVSVDNRGSWTIRLSNQVELRLGR
                     GEWTSKIDRFVTIFPEIHIPEGNRLAYVDLRYPHGAAVGFSPIKN"
     gene            12690..13961
                     /gene="ftsA"
                     /locus_tag="PXD81_000881"
     CDS             12690..13961
                     /gene="ftsA"
                     /locus_tag="PXD81_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005603039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HDL5536930.1"
                     /translation="MTKAVESKIIVGLDIGTSKVVAVVGEVLPDGVINVMGAGVCPSK
                     GVDRGGVIDLDAAVNSIQRAIEQAESIADCQIMGVTLAISGQHIVGLNEAGSSTLSGG
                     IVTQDNIESAVDMAKAIKLQDGLETLHMIPQEYHVDRLPATKNPIGLSGMRLQVQAHL
                     IACHQAWLKNLKNAVEHAKLKVDQVVFSGLASSYSVLTEDEKELGVCLIDIGGGTMDV
                     LVYTDGALRYSKVIPFAGNNITDYLARVFTTSRPEAESLKVGYGSAISPPTHNSDKKI
                     EVAGLGGRMARTFTRAQVATVTSQCYNDLLKVVEEELTQLRHELFQKGIKQELIAGVV
                     ITGGGSQIEDIVECAKSIFGSQVRVGYPLNITGLTDYVNKPQYATALGLLQYSHYNYT
                     EQGTSTDSNEDIFAPIGRGTKKIIDFFKSKF"
     gene            13994..15241
                     /gene="ftsZ"
                     /locus_tag="PXD81_000882"
     CDS             13994..15241
                     /gene="ftsZ"
                     /locus_tag="PXD81_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HDL5536931.1"
                     /translation="MFEPIIEQTQDAIIKVVGVGGGGGNAVDRMSRSADDIKGVEFFD
                     VNTDAQVLRKRTTRQTIQIGASTTKGLGAGADPMVGKQAAEEDREAIANALKGANMTF
                     IAVGMGGGTGTGAAPVVAQIAKEQGSLTVGVVTKPFRFEGPRRMRFADQGIKELSQYV
                     DSLIIIPNDKLRGLGKQTTAVDAFAAANDVLSNCVLGITNMITSSGGSTGADINVDFA
                     DVRTVMSGKGHAMIGTGFAEGEVGEGRAEKAMNDAISSPLLENVDISGASGMLINISA
                     GTDFLLEEVYAMMDLIYGFATEDAAIVFGCNYYPEMDGKVSVTLVATGIGQPEEALHM
                     PHKAQPVYAQQGNHVQATQPTQPNHAVQQPNFTQPSSYGQPNQFSGQPQPLQQPVAPK
                     PQSVDTAQIFNPNSIPGFMRNSQ"
     gene            15273..16193
                     /gene="lpxC"
                     /locus_tag="PXD81_000883"
     CDS             15273..16193
                     /gene="lpxC"
                     /locus_tag="PXD81_000883"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012073651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HDL5536932.1"
                     /translation="MTKQRTLKQAAKVTGIGLHSGKKVTLTLRPAPANTGVIYARTDL
                     EPVVYFPASADSIRDTQLCTCMVNDDGVRISTVEHLNAAMSALGLDNVIVEVDAPEIP
                     IMDGSSSPFIYLLLDAGIEEQDAPKKFIRIKETVRVEEGDKWAELKPYNKGFKLNFTI
                     DFEHPLIPKNVGSYSIDFSAENFIQQLSRARTFTFMKDVEYLQSIGLALGGSLDNAIV
                     LDDYRILNEDGLRFKDELVRHKMLDSIGDLYMCGYNILGEFSAYKSGHGVNNKLLRAV
                     LENKNAWEFVTFEDKAEVPQGYQVQQEIFI"
BASE COUNT         4848 a         3053 c         3798 g         4579 t
ORIGIN      
        1 ctaaaagtga aggttattgg actcagcttg cccaagttac aggtttggat tacaacagtt
       61 taatagaaaa agtacggaat aaccctagct cggttttttt acgtttagat aacgaaaatt
      121 tagtaagaga acgccagaaa ttccaagcgt taagcaaaga aatcggccgt tcctataatg
      181 acataatgga cgagctttat agcaaaaacc gccagcgatt cctttactta tcccgtcatc
      241 aatctgaagc gatcagcgaa tatgcaaaag aactagatat tgatgcatta gtgattaaag
      301 tcgaatcacg ccgtttttac ccgctggcag aagaatcttc acagttgatc ggttttactg
      361 ataaagatga tatgcacggt tcggaagggc tggagcgtag ttttgattcc ttacttatcg
      421 gcaaaaacgg taaacaaatt atccgaaaag atgccaaagg caatattgtg gaaaatatcc
      481 gttcggagaa acagtacgat ccgcaagatg ttatgttaag cattgatgaa gaattgcagt
      541 ctatggtgta cggtgaaatc aaaaaagcgg tacaggaaaa taatgcggaa tcaggcactg
      601 cggttttggt tgatgtgcaa accggcgaaa ttttggcaat ggcaaatgcc ccttcattca
      661 acccaaacaa acgggatagt tttaaacctg aattaatgcg taaccgtgct attaccgaca
      721 cttttgaacc gggttcaacg gtgaaacctt ttaccgtatt aaccgcatta caaaatgggg
      781 taacttatcg tgatgaagtt attggcactc gcccatttgt cgtcaatgga catacaatta
      841 aagacgttgc tccaagggac agccttacgc ttaccggcat cttgcaaaaa tccagtaaca
      901 tcggggtcag taaattagcc ctcagaatgc cggcaaatgc gttagtggat acttacacga
      961 aagtcggttt cggtaaagac accggtttag gcttgggtga acaacgaggt acgaacggtg
     1021 atcgcaaacg ttgggcggat attgaacgtg ctaccctttc ctatggatac ggtttaaatg
     1081 tgaccccatt acaacttgcc cgtgcttatg cgactttagg aagtttcggg ctgtatcgcc
     1141 cactttcgat taccaaggtc gatccgccgg tgattggcga acgggtgcta ccggaaaaaa
     1201 ccacccgaga tgtcgtgcat atgatggaaa gcgttgcggc taaaggcgaa ggcggacacc
     1261 gtgcagcggt ggacggctac cgtgtagcca tcaaaaccgg tacagcacgc aaattagaaa
     1321 aaggtaaata tgttgaaaaa tatattgctt ataccgcagg gcttgccccg gcaagcgatc
     1381 ctcgctttgc attagtggta ttaatcaacg aacctaaagc aggcaactat tatggtggct
     1441 ttgtttctgc accactgttc tcacgcatta tgggttacac cttaaaacaa cgtaatatta
     1501 agccggataa cttaacggaa aatacccaaa gtgccgtgcg tgaaattaaa ttagaacgcc
     1561 ctatcaatta aggttagcta tgaaacgttt actccctttt ctgacagaac ttgatgcttg
     1621 ggtggaagaa ctcaccgagc taaaacaaat gacacttgat agtcgccaag ttcaagccgg
     1681 tgatctcttt gttgctttaa aaggacatca agtcgatggc agaaaattta ttccgaatgc
     1741 gatagaacaa ggagcagcaa ttattttggc tgaggctgat gaagatcaac cggaaattga
     1801 gcttgatccg aaatttgcaa aatttaacct agatcgcacc gcttgttgca aggttgtctc
     1861 tgttccacgt ctgccaaaat tgctctctga aattgcagga gctctttacc ataacccgtc
     1921 acacaagctg actttagccg gcattaccgg cacgaacggt aaaacgacta ctgctcaatt
     1981 attagcacaa tggcataatt tattgggtgg gaaatcggca gtaatgggta ctatcggcaa
     2041 cggtttgtac ggacaagtgc aagaagcggt aaataccacc ggctctgcca ttgaaattca
     2101 gcataatctt gcgaactttg ttgaacttgg agcggatttt tgtgcgatgg aagtcagctc
     2161 tcacggtttg gctcaattta gagccgaagc cttggatttt gatttagcga tctttacgaa
     2221 tttaagccga gatcaccttg attaccacaa cacgatggaa gagtatgccc aagccaaatt
     2281 ccgtctcttc aacgaattaa gcaccaaagc tcaagtaatt aacgccgatg atgaaatcgg
     2341 gcgtgaatgg ctgacacaat taccaaatgc agttgcggta agcaccgatc cgaagtttgc
     2401 aggcaaccac caatttgtca aagccactgc ggtaaaattt accctacaag gggcaagtat
     2461 tgcgtttgaa tcaagttggg gcaatggcga gttacatagc cgtttaatcg gggctttcaa
     2521 tgtaaataat ctgcttaccg cctttgccgg acttttagct cttggctttg atattcaaga
     2581 actggttaaa accgccccga atttagttgg cgtagcaggt cgaatggaat gcattaccgc
     2641 tccaaataag ccaatggtaa ttgtggacta tgctcacacc cctgatgctc ttgaaaaagc
     2701 cctacaagcg gctcgtttac attgcgaagg cgaactttgg tgcattttcg gctgtggtgg
     2761 agaccgtgac gccggcaaac gcccattaat ggcgacgatt gccgagaaat taaccgataa
     2821 agtgattgct acagacgaca accctcgcac cgaagataat caaaagatta tggcagatat
     2881 tgtgaaaggt tttagtaaac cccaacaaat tatccacaat cgtgaagagg caattaaaac
     2941 cgccattgag caagctaaag ccacagatgt gattctaatc gcaggcaaag gtcacgaaga
     3001 ttatcagatt attggcacag agaagttaca tttttcggat caggaaacgg caaggaaata
     3061 tctgttttaa tgtcactttc tttgcacaag caaagaaaag tgacagtgac aaatacaaaa
     3121 tgctcgtaat taattttata aagaaaaaga taaatcaatg ataaaactaa ccacccaaca
     3181 aatcgctgaa atcctaaatg ccaaattgat cggcaatggc gaaactgtgg tagaaaccac
     3241 cagcaccgac acccgacaag cggttgaaaa cggcttattt tttgcattaa agggcgagaa
     3301 ctttgatgct catcactatc ttcaaaatgc agtcgcacaa ggttgtgtag cggttgtggt
     3361 tgagcgtgag tgtgaaattg atgtaccaca acttgtggtg gcagataccc gtttggcatt
     3421 gggggaattg gcgaaatggt taaaagctaa gctgaacccg aaaacggtag cgatgacagg
     3481 ttcttccggc aaaacaaccg tcaaagagat gacggcaaaa attctgcaaa aaatgaccgc
     3541 ttgcgaacag gaagtgcttt atacttttgg taacttaaac aatgacttag gtgtaccgat
     3601 gaccttgctt cgcttaaccg aacagcataa atatgcggtc attgagctgg gggcaaatca
     3661 tatcggtgaa atcgcttata ccaccgctat cgctcagcct gatgcctgct tggtgaataa
     3721 tgtggctgcg gcgcatttag aggggtttgg ttcattagca ggcgtggcac aagccaaggg
     3781 cgaaatttat cgtggcttga aagaaaatgg caaagcgatt gtcaatttag ctttttatta
     3841 tccgcaatgg caaaaagaga ttggctcacg ggaaatgcaa tctttctctt atatcgggtc
     3901 agattcggat tcttacgcag attactgggc ggaagatgta gaactgcatt taagcggttc
     3961 acgctttacg ctacataccc cacagggcga aattgctatt gatctgcctt atttgggcaa
     4021 acataacgtg agcaatgcct tagcggcaac ttcgctggcg atgtctgttg gggcaacgct
     4081 agaagcagtt aaggcagggc ttgagcagcg ttcaatggta aaagggcgtt tgtatccggt
     4141 gcaaattaac gaccactgtt tgtttattga tgacacttac aacgccaatg tggattcaat
     4201 gaaatcggcg atttctgttt tggaaaatta tccggcttat cgtatttttg cagtgggtga
     4261 tatggcggaa ttgggtgaag aaagtagcaa atgccaccaa gaagtagcgg attttgccaa
     4321 aaatgcagca ttagatttaa tcgtgagctt tggaaaagaa agtgcggtaa tcagccaaca
     4381 ggcagaacat cattttaccg ataaacagca aatggttgat ttcctactgc cgattatttt
     4441 gcaaaaaatc gaacaaaaac aaccgcttgt attattggca aaaggctctc gtagccagaa
     4501 aatggaaacc gtgatttccg aattgtgtaa accgtttaac ctaaatttct aattattttt
     4561 aaggactaag caatgttagt ttggcttgca gaactattgg ttcaatataa cacagcgttt
     4621 aacgttgtct cttatattac tttccgtgcc attatggcgt tattaactgc aatgggaatc
     4681 gggctttgga tcgggccgaa agtaattcgc cgtttgcaga tcttaaaatt tggtcaggaa
     4741 gtgcgtaatg acggaccgga aagccatttc aaaaaacgtg gtacgccaac aatgggcgga
     4801 gtgatgattc tcgcagcgat tggggtgagt acattgcttt gggcagatct gcgtaattcc
     4861 tatgtttggt tcacgctgtt tgtgctattt ggctacggtg cagtgggctt tgttgatgat
     4921 tatcgtaaga tctctcgtaa aaataccgat ggtttgattg cccgttggaa atatttttgg
     4981 ttatctgcta tcgcattagt ggcggtgttt ggtatgtatg cggtgggtaa agataccgct
     5041 gcaacacaat tagtcgtgcc gttttttaaa gatgtgatgc cgcaactcgg tttattttat
     5101 attgtgttat cctatttcgt gattgtcggc accagtaatg cggtaaacct aacggacggc
     5161 ttagacgggc ttgcgattgt gccaactatt atggtcgcat ctgcctttgc gttaattgcg
     5221 tgggcaaccg gtaactttaa ttttgcacaa tatttacata ttccttttat tccaaatgcc
     5281 ggtgaattgg ttattttatg tactgcgatt gtgggagcag gtttagggtt cttatggtat
     5341 aacacctacc ctgcacaggt gtttatgggc gatgttggct cactttcgct cggcggtgca
     5401 ttaggcgtga ttgcagtatt agttcgccaa gaattattat tggtggtaat gggcggtgta
     5461 tttgtggttg aagccctctc ggtgatttta caagtaggtt catacaaatt acgccaaaaa
     5521 cgtattttcc gaatggctcc aattcaccac cattttgaac tcaaaggctg gtctgaacca
     5581 cgggttatcg tgcgtttctg gattatcacg ctaatgctgg ttttagtcgg cttagtaaca
     5641 ttgaaattac gttaattgat tgaataaggt gcattgagat aatgtacctt atgttatttt
     5701 taaattacag aataaattat gcaaaaacaa tacgaaaaca aaaccgttac tattatcggc
     5761 ttaggtacaa cagggttatc ttgtgtggat ttctttaaga aaaaaaatgc caatatccaa
     5821 gtgattgaca cccgagcaaa cccagccgga gcggataaat tggacgtaac tattccgttg
     5881 cataccggtg ggttaaacag tgaatggttg ctgaaatctg atcttattgt aattagccca
     5941 ggtttagccg ttgccacacc tgaaattcag caagcgattc aagctggtgt tgaaatagtc
     6001 ggtgatgttg aactgttttg tcgggaagcg aaagctccga ttattgcgat caccggttca
     6061 aacggcaaaa gtaccgtaac gactttaact accgaaatgg cgaagcaagc ggggattaaa
     6121 gtgggaatgg gtggcaacat cggtgtgccg gtattaagtt tgctggaggg cgattatgac
     6181 ctttttgtgc tggagctatc cagcttccag cttgagacaa cttattcact gaaagcaaaa
     6241 gctgccactg ttttaaatat tagcgaagat catatggatc gctacgattc gattgccgac
     6301 tatcgccaag cgaaattgcg gatttacgat aatgcggaaa atatcatcgt aaacggtgaa
     6361 gatgcccaaa cttacccgat acaagcggtc aaaaaagtga ttcgttttgc agaacaaaat
     6421 gcggaatata gcctacgcaa tcagatgctt tattcaggcg atgatgcaat tatccaaact
     6481 aaacagatgc tgattagcgg ccgtcataat gagatgaatg cgttagcggc gatggcatta
     6541 gcagaagcgg cgaatgttcc tcgtgaaggc attgtgaaag ctttgcaaat ttatggtggt
     6601 ttgccacacc gtttccaagc ggtggcaacc aatgatggcg tgcgttgggt caatgattcc
     6661 aaagcgacca atgtgggtag cacggtagcg gcattaaacg gcttgaatat tgccggtaca
     6721 ctttatttac ttttaggtgg tgacggcaag gctgcggatt tttccgaact caaaccatta
     6781 attaataagc caaatattgt ctgctattgt ttcgggcaag atggagcggc attggcagaa
     6841 ttaagcgaac aaagtgtgtt ggttgaaacg atgcagcaag caattgaagc aatccgccca
     6901 caactgaaaa aaggcgatat ggtactgctt tctccggctt gtgccagttt agatcagttt
     6961 aaaaactttg agcaacgtgg cgaggtattt atggcattag cacaaggaca gcacgcataa
     7021 tgttagaaag aattaagtcg gaatgggata aatgggtcag attaacccct tctaattcac
     7081 tttatgacag aactctaatt tggctatttt taggcctgct aattatcggt tttgtgatgg
     7141 taacatctgc ttcactccca gttagcacaa ggttgaataa cgatccattt tattttgcga
     7201 ttcgagacgg gctgtatatt attgcttcaa ttatcttttg ttatgttttt gtacaaatcc
     7261 caattgaaaa atgggaaaag cataacttgg cactgttttt tatttcaatc ggttttttaa
     7321 tcgccgtact cattttcggc cgctcaatta atggtgcagt gcgttggatt ccgttgggta
     7381 ttctcaactt tcagccggca gaattagcca aattagcggt catttgctat tttgccagtt
     7441 tctatgtacg taaatatgat gaaattcgta aagaaaaagc cagtttctgg cgaccggcag
     7501 tgattttatt cttattcggc ttcttgctga ttttgcagcc ggatttaggt agtacctttg
     7561 tattatttgt tctcactttc tcaatgcttt tcattgtggg ggcaaaaatt atgcaattta
     7621 tgtttttagg cgtggtggga acggtgttat ttgcggtgtt gattttaacc tcggaatacc
     7681 gtttaaaacg tgttacctcc tttatggatc cgtttgctga tgcttatggc gacggtttcc
     7741 agctctcgaa ctcacaaatg gcatttggac aaggtgaatt ttggggacaa ggtttaggta
     7801 attcggtaca aaagctagaa tatttgccag aggcacatac tgactttgta atggcggtta
     7861 ttggtgaaga atttggtttc tttggtattg cttgcatcgt acttttactt attctattaa
     7921 cggtgagagc cttaaaaatc agtaaagaat ctttggtgct ggaagagcgt tttaaaggct
     7981 atatggcatt tgggattgcg atttgggttt tcctacaagg cttcgtaaac ttaggggtgg
     8041 catcaggttt gctgccaacg aagggcttaa ccttcccatt agtgagttat ggtggttcga
     8101 gtttggtaat tatgtcgatt gcgattgcag ttttgctacg aattgaccac gaaaaccgtg
     8161 ccgagcgaat cgggcacgca aaaattaaaa ccatctagcc gagcggtctg caacgttaca
     8221 atttgcaaaa ttttacaaaa atgagaccgc ttgtattagc tggaaagcat aaaaattcag
     8281 taaaatagca cgttttagaa taaaaaagag ttgaaaatgg cgaagaaatt attagtaatg
     8341 gcaggtggaa caggggggca cgttttccct gcaattgccg tagctcgaga gctacaaaaa
     8401 caaggctggc aaatccgctg gctggggacg aaagaccgaa tggaagccac tcttgtgccg
     8461 aaacacggta ttgagattga ttttattgaa atctcaggct tgcggggtaa aggcgttgca
     8521 gcattgttga aagcaccgtt tgctattttt aaagcggtga tgcaagcccg caaaatcatt
     8581 aaaaattatc agcctgatgc ggtgttggga atgggcggtt atgtgtccgg tccgggcggt
     8641 attgcggcta aattatgtgg cgtgccggtg attttacacg agcaaaacgc agttgccggc
     8701 ctaaccaatg tgtggctatc caaaattgcc cgcaaggtgt tacaggcttt ccctactgct
     8761 tttaaagagg ccgaagtagt aggtaatcct gtacgtgaag atttatctgc tcttccgctg
     8821 ccgaatgaac gtttcgccaa acgaggctac ccgattaaca ttttagtaat gggtggcagc
     8881 caaggggcaa gagtgatcaa ccaaaccgtg ccggaagtgg caaaagtgtt gggtaataat
     8941 gtgtttatca gccaccaagt cggtaaaggt aatttagccg gtatcgaaga agtctatcaa
     9001 caaaccggca atgggattgc ttccgagttt attgatgata tgaaagccgc gtatgagtgg
     9061 gcagatttgg tgatttgtcg ttccggtgcg ttaacggtgt gtgaaattgc cgccgtaggt
     9121 ctgcccgctg tgtttgtgcc gttccaacat aaagacaggc aacaattttt aaacgctaac
     9181 tatttggcgg cagatggagc agcagtgatt atcgaacaga gcgatttcac tccaaaggca
     9241 ttacagcaag ccttagaacc cttaattgcc aatcgccaac tcttattgga tatgtcaatg
     9301 cgagccagag caaaatccac cccaatggca gcaaaacgtg tagcggaagt gattattcaa
     9361 gaagcgaatt aacttactac aagcggtcgt ttttggtaaa aattttgcaa aatgtaaccg
     9421 cttgcgaata gattaaaagg acaataaatg aaaaatttcc aagataaagt caaaaaatta
     9481 gtgcctgaaa tgcgtcgtgt cagccaaatt cattttatcg ggattggcgg agccggtatg
     9541 agcggcattg cggaagtgtt attaaacgag ggataccaaa tttcaggttc ggatattgca
     9601 gacagagcgg ttacacaacg tctatccgca gccggagcga aagtctttat cggacaccaa
     9661 gcagaaaata ttgctggggc gagtgtggtg gtggtttcta ctgcgattga tgaaaccaac
     9721 cctgaaatta ttgccgctcg agaagctcga attccggtga ttcgccgagc agaaatgctt
     9781 gccgaaatta tgcgttttcg ccacggcatt gcgattgcag gcacacacgg taaaaccact
     9841 acaaccgcaa tgatttcaat gatctacacc gaagcaaaat tagatccgac tttcgtgaat
     9901 ggcggtttgg taaaatctac cggcaaaaat gctcatttgg gggcgagccg ttatttaatt
     9961 gccgaagccg atgagagtga tgcttctttc ttgcacttgc agccaatggt gtcggtagta
    10021 accaacattg agcctgacca tatggacacc tatggtggcg attttgagaa aatgaaagag
    10081 acttacgtac gatttttgcg taacttacct ttctacggct tagcggtaat gtgtgccgat
    10141 gatgaaacgg taatggaaat tgctcctcgt gtcggcagac aagtgattac ctatggcttt
    10201 agcgaaaatg cggattaccg aattgaagat taccagcaaa ccggcttcca agagcattac
    10261 accgtagttt gcccaagcgg tgaacgaatt gatgtgctgt tgaatgtgcc gggtatgcac
    10321 aatgccttaa atgcaaccgc tgcgttagcg gtggcaaaag aagaagggat tggcaatgag
    10381 gcgattttag ctgctttagc tgatttccaa ggggcaggac gccgctttga ccaattaggt
    10441 tcatttattc gcccgaacgg caaaattatg ttagttgatg actacggcca ccacccaact
    10501 gaagttgatg taaccattca agccgctcgt tcaggctggg aaggtaaacg tgttgtgatg
    10561 attttccaac cgcaccgcta ttctcgtacc cgtgatttat tcgatgaatt tgtacaagta
    10621 ctgtctaaag tcgatgcctt gattttattg gatgtgtatg ctgccggcga agctccgatt
    10681 gtgggggcag acagtaaagc cttagcccgt tcaatccgta atttaggtaa ggtcgatccg
    10741 attttggttt cagataccga tcaacttggc gaggtattag atcaaattat tcaagacggt
    10801 gatttaattt tggcacaagg tgcgggcagt gtaagccgca tttctcgtgg cttggctgag
    10861 agctggaaaa attaaatact ctctcccctt gcgggagaga gaccgaaaaa tacttcgttc
    10921 agaagtattt ttcagagaga gggtatatat ttaaaggatt tcaaatgagt attaaaaacg
    10981 aaaaaattgc cgtcttattc ggtggcgttt cgcaagagcg tgaagtttca ctgaattctg
    11041 gtgcagcggt aacagctgca ttaaaaagtt taggctataa cgtagaaggc attgatacta
    11101 aagaattccc gattgaaaaa ttaaaagaaa aagggattca gcgtgtattt aatattttac
    11161 acggcggtat tggcgaaaat ggcgtactgc aaggtgcgtt agagcaaatg ggtattcctt
    11221 acaccggttg tggcgtaatg gcatcagcag tgacgctgga taaattccgg actaaattat
    11281 tatggaatgc agtaggttta ccaacagcgg atatggtggt ggttcagcgt ggacaagcgg
    11341 tcgattttga ccaaattatt gcaaaattag gcttaccggt atttgtgaag tcttcgtgtg
    11401 aaggctcaag tgtgggtgta ttcaaagtaa aaaccaaaga agaattggca ccggcaattc
    11461 acgaggcatt aaagtttgat tccattgtgt tggtggaaga atttttagcc ggtaatgaat
    11521 attctgtacc ggtattagac ggacaagttc taccggcggt acaagtaatt ccggacggcg
    11581 aattttatga ctaccacgca aaatatgtgt cggataacac ccaatatgta gtaccggcat
    11641 taaatgacga acgccaagca gaggtcgcca aattagtgaa agcggcttac gatgtggttg
    11701 gctgtcgagg ttggagccgt attgatgtga tggaagatgg cgaaggaaaa ttccgcttgg
    11761 ttgaagtaaa taccaacccg ggaatgacca gtcacagtat tttcccaaaa tcagcagcaa
    11821 cagttggtat ttcctttgaa aaattagttg aacgcgtatt ggagttaagt gcttaatggg
    11881 catcttccgc aagaagaaca gcacaaatgt gcgtttgaat gttcttaatc caaaaacacc
    11941 tgtttctcca cgtaaatggc tagtattcat taagccatta attgtgttat tatgtacact
    12001 atttgtattc ggtgtgtata gtaattggca aaatatactc gaaagttggg ataaaacacc
    12061 gattcgcgct tatgcattaa cacataaaac acaatttaca acaaatgcag atattcgtga
    12121 aactttgtct aaagaacctg ctctaaaagg atatttcgga caaaatattc aagaggtcaa
    12181 agataagctt ttatctatac cttgggttaa agatgtggct gttcgtaaac tttatcccga
    12241 ccgattaagt attactttgc tagaacataa acccgttgca atttggaatg atactaactt
    12301 tgtctcagaa caaggaaccg tctttagttt accgaaagac agaatcgata aaaatggact
    12361 tccattactc tatggtccag atacggaagg aaaaagtgta ttagaggcat ggaataaaat
    12421 ccgagctgat ctaaatgccc gcaacttgga tttaacctcc gtttctgtag ataatcgagg
    12481 ttcttggaca attaggcttt ctaatcaagt agaattaaga ttaggaagag gggaatggac
    12541 ttctaaaatt gatcgttttg taacaatctt tcctgaaatt catatacctg aaggtaatag
    12601 attagcttat gttgatttac gttatccaca tggcgcggcg gttggattta gtcctataaa
    12661 aaattaaact cagtgaacac aaaaacatta tgacaaaagc agtagaatca aaaattatcg
    12721 ttggtcttga cattgggaca tcaaaggttg ttgccgttgt tggtgaagta ttacctgatg
    12781 gtgtaattaa tgttatggga gctggcgttt gcccttctaa aggagtcgat cgaggtggcg
    12841 ttattgatct tgacgctgcc gttaattcta ttcaaagagc cattgagcag gcggaatcta
    12901 ttgccgattg tcaaattatg ggtgtaacac tggcaatctc aggtcagcat attgttggtt
    12961 taaatgaagc aggatcatca acattatctg gaggtattgt tacccaagat aatattgaaa
    13021 gtgctgtgga tatggcaaag gcaattaaat tacaagatgg cttagaaaca ttacacatga
    13081 ttccacaaga atatcacgtg gatagattgc ctgcgactaa aaatccaatt ggcttatcgg
    13141 gtatgcgttt acaagttcaa gcacacttaa ttgcttgcca tcaagcttgg ttaaaaaact
    13201 taaaaaatgc ggttgaacat gctaaattaa aagtcgatca agttgtattt tcaggtcttg
    13261 cctctagcta ttctgtttta actgaagatg aaaaagagct gggtgtttgc ttaattgata
    13321 ttggtggcgg tacgatggat gtactagttt ataccgatgg agcattaaga tacagtaaag
    13381 tcattccatt tgcaggtaat aacattactg attatttggc tcgtgtattt acgacatctc
    13441 gccctgaagc tgaaagcctt aaagtaggtt atggtagcgc aatttcgcct ccaacccata
    13501 attcagataa aaaaattgag gtggcaggac taggtggacg gatggcaaga acctttaccc
    13561 gagctcaggt ggcaacggtt acctcacaat gttataacga tttgctaaaa gttgttgaag
    13621 aggaattaac ccaattacgc catgaacttt tccaaaaagg aataaaacaa gagttaattg
    13681 ccggtgtagt cattactggt ggtggctcac aaattgaaga tattgtagaa tgtgctaaat
    13741 caatttttgg ttcacaagtt cgtgttggat atccactcaa tattactggc ttaaccgatt
    13801 atgtgaataa accacaatat gcaacagcat tagggctttt acaatatagc cactataatt
    13861 atactgaaca aggtacaagt acagattcta atgaagatat ctttgctcct attggtcgtg
    13921 gaaccaagaa aatcattgac ttcttcaaat caaaatttta atagtgttaa aataaacacg
    13981 cagggagata aaaatgtttg aacctattat tgaacaaacg caagatgcta tcattaaggt
    14041 agtgggtgtt ggtggcggtg gcggtaatgc cgtagacaga atgtctcgct ctgccgatga
    14101 tattaaaggt gttgaattct ttgatgtaaa tactgatgct caagttttaa gaaaaagaac
    14161 cacacgccaa actatccaaa ttggagcaag tacaactaaa ggtttaggtg caggtgctga
    14221 tccaatggta ggaaaacaag ctgccgaaga agatcgtgag gctatcgcta atgcattaaa
    14281 aggtgcaaat atgaccttta ttgctgttgg tatgggtgga ggtaccggta ccggagcagc
    14341 ccctgttgtt gctcaaattg ccaaagagca aggctcacta actgtaggcg ttgtaacaaa
    14401 acctttccgc ttcgaagggc ctcgccgtat gcgctttgca gaccaaggca ttaaagagct
    14461 ttctcaatat gttgattctc ttattataat tcctaatgat aaattacgtg gtttaggtaa
    14521 gcaaacaaca gcagtagatg cctttgccgc agcaaatgat gttttaagta actgtgtgct
    14581 tggtattacc aatatgatta ccagttcggg cggttctacc ggtgcagata ttaacgtgga
    14641 cttcgcggat gttcgtacag taatgtctgg taagggacac gctatgattg gtacaggttt
    14701 tgctgaaggt gaagttggtg aaggacgagc ggaaaaagca atgaatgatg ccatttctag
    14761 tccattactt gaaaatgtgg atatttcagg tgcaagtggt atgctcatca acattagtgc
    14821 tgggacagat ttcttattag aagaagttta tgctatgatg gacttaattt atggctttgc
    14881 aacagaagat gccgcaattg tatttggttg taactattac cctgaaatgg atggaaaagt
    14941 gagtgtcact cttgtcgcaa caggaatagg ccaacctgag gaagctctac atatgccaca
    15001 taaggctcag ccagtatatg cacagcaagg aaatcatgtt caagctacac agcctactca
    15061 gccaaaccat gcggtacaac aaccaaactt tactcaaccg tcaagttatg gtcagccaaa
    15121 tcaatttagt ggtcaaccac aaccactaca acaacctgtg gctccaaaac cacaatcggt
    15181 tgatacagca caaattttta acccaaattc aattccgggt tttatgagaa atagtcaata
    15241 acttccactc atttcggaag taaggacaaa atatgactaa acaaagaaca ttaaaacaag
    15301 cagcaaaagt aacaggtatt ggcttacata gcggcaaaaa agtaacctta acgttacgcc
    15361 ctgccccagc taatacagga gttatttatg cccgtaccga tttagagcct gtcgtttatt
    15421 tccctgcaag cgcagactct attcgtgata cacaattatg cacatgtatg gtaaatgatg
    15481 atggtgttcg tatttcaaca gtagaacatt taaacgcagc aatgtcagca ttaggcttag
    15541 ataatgttat tgttgaagta gatgcacctg aaattccgat tatggacggt agttcaagcc
    15601 catttatcta tcttttatta gatgccggca tcgaagaaca agatgcaccg aagaaattta
    15661 ttcgcattaa agaaacagta cgcgttgaag aaggcgataa atgggccgaa ttaaaaccat
    15721 acaataaagg ttttaaattg aacttcacta ttgactttga gcacccatta atccccaaaa
    15781 atgtagggag ctattccatt gatttttctg ctgagaactt tattcagcaa ctaagtcgag
    15841 cgagaacctt tacatttatg aaggatgtag aatacttgca atctattggt cttgcattag
    15901 gtggaagcct tgataatgct attgtattag atgattaccg aattctaaat gaagacggtt
    15961 tacgatttaa agatgaactg gttcgccata aaatgctgga ttcgataggt gacttatata
    16021 tgtgtggtta taatatccta ggtgaattta gtgcatataa atcaggtcat ggcgttaata
    16081 ataaattatt aagagctgta ttggaaaata aaaacgcttg ggagtttgtt acctttgaag
    16141 ataaagcaga agtcccacaa ggttaccaag tacaacaaga aatttttatt tagtctaaat
    16201 ggcttaaaag acgaacagaa atgttcgtct tttattttgc aaattttttc taatatttaa
    16261 ccgcttgcaa cccgcctc
//
LOCUS       DANLYI010000038        16105 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.038, whole genome shotgun sequence.
ACCESSION   DANLYI010000038 DANLYI010000000
VERSION     DANLYI010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 16105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16105
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.038"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            <1..275
                     /locus_tag="PXD81_000884"
     CDS             <1..275
                     /locus_tag="PXD81_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5536933.1"
                     /translation="LDEWYSASDKERREFIAKHFAEETVRSDRNTPYDYAMCTGRMFA
                     KQLTRDEVKDYPLANTKPQYLTRDRRIEIMSNLPREILQNCAKYIQ"
     gene            complement(311..1063)
                     /locus_tag="PXD81_000885"
     CDS             complement(311..1063)
                     /locus_tag="PXD81_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HDL5536934.1"
                     /translation="MAYKPPFTVTTKMLNLVVEISHKLGTLQLDFERNLHLRKNNRLR
                     SIQASLAIEANSLTIEQVTAIIKGKKVLGHPKDIREVKNAYDAYDEMMHYNPYSIKDF
                     LKAHKFLTADLVNDAGAFRSKDVGIYNFSGQLVHMGARPQFVENLINELFSWAKQDDT
                     PALIKSSVMHYEIEMIHPFSDGNGRMGRLWQNVLLAQWNPLFAWLPIETMVYENQQRY
                     YQMLSLADSQNDSTGFIEFMLEMILETLLKLK"
     gene            complement(1102..2349)
                     /gene="clpX"
                     /locus_tag="PXD81_000886"
     CDS             complement(1102..2349)
                     /gene="clpX"
                     /locus_tag="PXD81_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HDL5536935.1"
                     /translation="MAFEKEPHCSFCGKKRTEVDQLVEGTEGYICNECIEESYALLHG
                     EEEPLIDENQDDEGSFFTNVPTPHELHAHLDDYVIGQEHAKKVLSVAVYNHYKRLRSA
                     LSNHQATNGVELGKSNILLIGPTGSGKTLLAETLARRLNVPFAVADATTLTQAGYVGE
                     DVENVIQKLLMKCDFEAEQAERGIVFIDEIDKITRKSESASITRDVSGEGVQQALLKL
                     LEGTVANINPQGGRKHPKGETIPVDTSKILFICGGAFAGLDKIVEARTNTQGGIGFAA
                     ALKKDKDRQDLTELFKQVEPEDLVKFGLIPELIGRLPVVTPLQELDEEALIQILTEPK
                     NAIIKQYQALFEMEGVELHFTKDALISIAQKAISRKTGARGLRSIVENLLLDTMYDLP
                     TLNAKKVTIGKGCVENGDKPKVE"
     gene            complement(2349..2942)
                     /gene="clpP"
                     /locus_tag="PXD81_000887"
     CDS             complement(2349..2942)
                     /gene="clpP"
                     /locus_tag="PXD81_000887"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HDL5536936.1"
                     /translation="MALVPMVVEQTSKGERSYDIYSRLLKERIIFLNGQVEDNMANLI
                     VAQLLFLEAEDPEKDIYLYINSPGGVVTAGLAIYDTMNFIKPDVATLCTGQAASMGAF
                     LLSAGAKGKRFALPHARIMIHQPLGGARGQATDIQIQAEEILRLKATLTRRMAEHSGQ
                     SYEKVLADTERDNFMSAEEAAEYGLIDKVLTSRSEVA"
     gene            3054..3437
                     /locus_tag="PXD81_000888"
     CDS             3054..3437
                     /locus_tag="PXD81_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufE family protein"
                     /protein_id="HDL5536937.1"
                     /translation="MTLTEIYQKFERCKSWEERYRLLIQLSRLLPKPSEQELAQIAEI
                     EGCESRLWFEFQAEPRKVVAYSDARLMQGILFIVSVALTEKTAEELKGFDLKTMFDEL
                     KISQNLTSTRLNGLGQLQKHIMMNL"
     gene            complement(3541..3617)
                     /locus_tag="PXD81_000889"
     tRNA            complement(3541..3617)
                     /locus_tag="PXD81_000889"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3581..3583),aa:Asp,seq:gtc)
     gene            complement(3706..4461)
                     /locus_tag="PXD81_000890"
     CDS             complement(3706..4461)
                     /locus_tag="PXD81_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDL5536938.1"
                     /translation="MKKSIYDTPIFFERYQQLRQNPISMNEVVEKPTMFSLLPDLTGK
                     KVLDLGCGTGVHLAHYLTLGAEKVVGLDLSELMLQQAESDLSAHWQKGNDFSLYCLPM
                     ERLNEISEGDFDVVTSSFAFHYIADFSELLAKISQKMTACGTLIFSQEHPIVTCYREG
                     YRWEKNQEKQQVAYRLNYYREEGERDRSWFQQPFKTYHRTMATIVNNLIQGGFEIVQM
                     EEPMLAGQPRWHNEFKDLQHRPPLLFIKAIKQK"
     gene            complement(4521..5516)
                     /gene="trpD"
                     /locus_tag="PXD81_000891"
     CDS             complement(4521..5516)
                     /gene="trpD"
                     /locus_tag="PXD81_000891"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HDL5536939.1"
                     /translation="MQALLDKLFANQALAQAESHRFFSEVIKGNVSNEQLAAALIALK
                     LRGETVDEIAGAAKAALENANPFSTPDYPFADIVGTGGDGHNTINISTTSAIVAATMG
                     YKIAKHGNRSVSSKTGSSDVLSHLGINVDVSPETARLALDETNICFLFAPTYHSGFKY
                     AAPVRQALKTRTIFNILGPLVNPAHAKRQLLGVYSPELVKVYAETVKALGYEHTIVVY
                     GAGLDEVAVHGETQVAEIQNGQIDYYTLTPEDFGIERHSLEDLKGGEPAENAEKVTAI
                     LQGKGKLAHIDAVAANVALLMKTFGEDDLKANVQKVKETLESGKALETLRKLSTY"
     gene            complement(5534..5920)
                     /locus_tag="PXD81_000892"
     CDS             complement(5534..5920)
                     /locus_tag="PXD81_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005543439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase family protein"
                     /protein_id="HDL5536940.1"
                     /translation="MISVYGLKQTLADRRALIADVIFDCMQMSLGVPKQRHALRFDLL
                     DAENFYPPINRSQDFIVIEINLMQGRSEQLKKRLIKGLFSALHTKVGIVPMDVEITIK
                     EQPEHCWGFRGMTGNEATDLEYQAHR"
     gene            complement(5913..6503)
                     /locus_tag="PXD81_000893"
     CDS             complement(5913..6503)
                     /locus_tag="PXD81_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HDL5536941.1"
                     /translation="MANILFIDNFDSFTYNLVDQFRELGHQVTIFRNDYPLEDFLAKA
                     ETTENCLVALSPGPGNPAEAGNLLEIIRRLKDKVPMIGICLGHQALIEAFGGKVVHTG
                     TVLHGKVSRIEHDNQAMFSGINNPMLVARYHSLMGDELPAEFIVNAKFEHIVMAIRHK
                     TLPICGFQFHPESILTVQGTKLLKQSVEWLLGEKND"
     gene            complement(6513..8069)
                     /gene="trpE"
                     /locus_tag="PXD81_000894"
     CDS             complement(6513..8069)
                     /gene="trpE"
                     /locus_tag="PXD81_000894"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HDL5536942.1"
                     /translation="MPQLTITQTELPYFADTTRVYYALCGERPHTLLLDSAEIQSKNS
                     VKSLLFAKSAVHIFCEDQTVTFHALTPNGEAVLPLIANALSLLNPAPTCKFLDKNDRL
                     QAIFPTISNQLDEDSKLKTTTVFDGLRTVSQLFADSTSPIYLGGLFSYDLVANFIPME
                     NISLQDDGLSCPDYTFYLAEQLVTIDHQTETAVLNTFCFEPNEQTNLNQQAVEFTQKL
                     ANLSHIELPLANQTATSEATANIEDEDFKAIIRKLKHHINIGDVFQIVPSRRFSLPCP
                     NTLASYKQLKINNPSPYMFYMQGENFTLFGASPESALKYSQANRQLEIYPIAGSRPRG
                     FDEQGNIDPELDSRLELELRLDQKELAEHLMLVDLARNDVARVSESGTRRVVDLMQID
                     RYSQIMHLVSRVIGTLRKDLDALHAYQACMNMGTLTGAPKIKAMQLIYQVEQQKRHSY
                     GGAVGYLTSDGNLDTCIVIRSAFVQNGIAYVQAGCGEVLDSDPQLEADETRHKARAVL
                     KAIGQVNAKA"
     gene            8211..8828
                     /locus_tag="PXD81_000895"
     CDS             8211..8828
                     /locus_tag="PXD81_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014990994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HDL5536943.1"
                     /translation="MTQFFYIHPDNPQQRLINQAVEIIRNGGVIVYPTDSGYALGCAM
                     GDKHAMDRIVAIRKLPENHNFTLVCSDLSELSIYALVNNQAYRLIKNNTPNPYTFILP
                     ATKELPRRLMTKRKTVGIRVPDNAIALALISALGEPILSCSLMLPETEMTESDPDEIR
                     DHLQNRVDLIIHGGYLGQQPTTVVDLTDDVPVIIREGSGDPTSFN"
     gene            8974..10041
                     /gene="rluB"
                     /locus_tag="PXD81_000896"
     CDS             8974..10041
                     /gene="rluB"
                     /locus_tag="PXD81_000896"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HDL5536944.1"
                     /translation="MTTFQKKSTATFRADKRGESRPSFKKEEQKSERKFGKTSEKPPR
                     PTSKPLTQRIAKKVEKTTASEVASQEKKAKVVGEKLQKILARAGQGSRRELEEIIAAG
                     RVSVDGKMATLGDRVQVSSSTKIRIDGHLVNLMPAQKEICRVLMYYKPEGELCTRSDP
                     EGRSTVFDRLPRLSGARWIAVGRLDINTSGLLLFTTDGELANRLMHPSREVEREYSVR
                     VFGNIDDAMLQRLRKGVQLEDGPANFKQIKVVGGTGLNQWFDVTLTEGRNREVRRLWE
                     SQGVEVSRLIRIRYGNIKLDKGLPRGGWEEMGLEQVNYLRELVGLPPETETKVEVGVN
                     RRRTNIRQIRKAVKQHQKYRG"
     gene            10107..11879
                     /locus_tag="PXD81_000897"
     CDS             10107..11879
                     /locus_tag="PXD81_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HDL5536945.1"
                     /translation="MSWDIVATLAVTGIAVFLFATEKMRMDVVAILVLCSLVLLGQVD
                     TKSALSGFSNSATVTVTAMFVLAAGLQNSGALDSVGSLLAKVKSPWLFLLVLCGVNAA
                     VSPFVNNTAVVAVLIPIVIAAAQNIKMAPSKALIPLSFSSQMAGVCTLIGTSTNLLVN
                     AIAQKQGHSGFGMFEFAPLGIIFLVVGVAYLLVTSRFLLPESHLQLEDGEGFGKYVSE
                     LKVNKDSVLIGKSTFESGLNEEFNLFTIGVLRDGERLSTPSHQVLQKHDILLLRGESE
                     NLAKVREKYGLHHVVYGRRENDEDNLDEDLMVAEVMISPISRWIGGTIPILQQRWNKN
                     ATVLGIQRQSKVIRERLRTTAFKMGDILLLTLPKEDMETLRQDKDFIVLSSDLVKTEE
                     SWKSKFALGVMVLVVATAALGWVPISVSALIGAVAMCVAGCLTAEEAYQSIDWKIILV
                     LAGLLPLGEAMANSGAAQFIVDNTLGKVGEFGPLVVLAVLYLLTMVLTEFMSNAGTAV
                     LLTPIAVSTAKMLGVDASPFIIAVMFAAATSFMTPVGYQTNTMVYGAGGYKFTDFIKI
                     GLPLNVIYWILGIVLIPIFFPFNP"
     gene            complement(11969..>16105)
                     /locus_tag="PXD81_000898"
     CDS             complement(11969..>16105)
                     /locus_tag="PXD81_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HDL5536946.1"
                     /translation="RIVWNHAKRTWIVASELSRSANKSNATNTSLTTNIIKLSTLSLA
                     LSAGFASAATYSPTYGTIPDNNPQTSIAIGDGSKVNSTLVSTITGLEGNSGKVSTALG
                     YNTTATADFSTALGGFANASFASTAVGGQATADKRSVAVGYNATAMGLREVFIGDHAG
                     LNHSNSTEYNIGIGYSASSNVTGNNTISIGNTAGDGTSGSHNIAIGTYANAKLAGPTT
                     NVTSDDNIAIGNSALANGVNNYKTTAATGETVIGKATAVGSHANATGIVSSAYGAEAN
                     ATAIHSTAIGAKSQANGDNSTAIGYEAKTSGHGATSLGYYANATANLTTAVGTNAGAT
                     SDYASAFGREANASGGSATALGNRATASGAASVALGVSAKATNQRTIAIGESSNASAF
                     NATAIGRNATAEHTDSIALGSNSVTAIAIPTTNATVNGITYSDFAGTNPIATVSIGAE
                     GKERTITNVAAGRISLSSTDTINGSQLYLTQQAIGNVAATTANILGGGAAVTENGNIT
                     FPTYALVNGTPDADKEGKQGRYTTVSAALSALNTAVISPLTFAGDTGTNFERHLGSTV
                     KIKGGSTGILTENNIGVVADGNSTLTIKLAEKVNLGANGSLTTGDTVVNNTGITIANG
                     VADKPVSLTKSGLDNGGNKIANVAAGDVDTDAVNVSQLKQAISKFATHYVSISDDGIQ
                     RANYDNSGSSGVNPMAIGVATSANGELATALGSEAEANGERTTAVGPRATADGMNATS
                     IGYNANANATNALAVDSAANANADTSTAIGTASTATATRATALGSKSEATGENSTAVG
                     YEASSIGADSLAAGYNANASGTQSTALGNSANAGGIWSTSVGRNANAAGSSAIALGNS
                     ANAAGVASIALGVSSQATTTAAVALGQNAKATHQGSVALGTNSETVATVATKSATLNG
                     NTYTFAGTTPSSTVSIGSVGNERTLTNVAAGRILDSSTDAINGSQLYAAYTEIDGLNT
                     KVNELSNGALTFVDDAGTEIVRKLGTSLNVKGGADATILTDNNIGVVATDANTLTVKL
                     AKDIDLTPAGSVAVGNSKLNNNGLTINNGPSVTMTGVDAGKLKITNVADGDISPISAD
                     AVNGSQLYDTANTIATALGGNSSVNANGAVSAPSYTVVDGAPTNEVSKTVNNVGSAIT
                     ALNDAVTSPLTFAGDTGTPSQRKLGSTVTVKGGVSNESQLTDNNIGVISNGNGSLTVK
                     LAKDIKVNSVTAQTVTANIAVADTVKTGDTTIDTNGLTIVGGPSITKTGINAAGTKVT
                     NVKAGTEDTDAVNFSQLKATEKNINNKINNIDSKVNKVDKRLRAGIAGATATAGLPQA
                     YLPGKSMLATAGDTYRNEAAIAVGYSRISDNGKVIYKLTGNSNTRGDFGGSIGMGYQW
                     "
BASE COUNT         4426 a         3285 c         3457 g         4937 t
ORIGIN      
        1 atttagatga gtggtactca gcttcagata aagagcgaag agaatttatc gcaaaacatt
       61 ttgcagaaga aacagtaaga agtgatagaa atacaccgta tgattatgct atgtgcactg
      121 gtagaatgtt tgctaaacaa ctgactcgag atgaagtcaa agactaccct ttggcaaaca
      181 ctaagccgca atatttaacc cgtgatagac ggattgagat tatgtctaat ttacctcgtg
      241 aaatattaca aaattgtgct aaatacatac agtaatttta tgagtaacac cactttggaa
      301 ttaagtggtg ttattttaat tttaataatg tctctaaaat catctccaac ataaactcaa
      361 taaagccggt agaatcattt tggctgtctg cgagagaaag catttgataa taacgttgtt
      421 gattttcgta aaccattgtt tcaatcggca accaagcgaa aagtgggttc cattgtgcta
      481 aaaggacatt ttgccataat cgacccattc tgccgtttcc atcactaaag gggtgaatca
      541 tttctatttc atagtgcata accgagcttt taatgagtgc aggtgtatca tcttgtttag
      601 cccaagagaa caactcatta atcaaattct ctacgaattg aggacgagcc cccatatgca
      661 caagttgccc actgaaatta taaatgccga catcttttga acgaaaagcc cctgcgtcat
      721 ttactaagtc agcagtaaga aacttatgtg ctttgagaaa atctttaatc gagtaaggat
      781 tatagtgcat catttcatcg taagcgtcat aggcattttt gacttcacga atatctttgg
      841 ggtgtccgag aacctttttg cccttaatga ttgcggtaac ttgctcaatc gttaatgaat
      901 ttgcctcaat agctaaagag gcttgaattg atctcaagcg gttatttttt ctaagatgta
      961 aattacgctc aaaatccaat tgtagcgttc ccaatttatg agaaatttct acaaccagat
     1021 tcagcatttt agtggtaact gtaaaaggtg gtttataagc cattttgcaa taatcctaaa
     1081 aaattaaacc gcttgtcatt gttactccac cttcggctta tcgccgtttt caacacagcc
     1141 tttgccgatg gtgacttttt tcgcattgag ggttgggaga tcgtacattg tgtcaagtaa
     1201 taagttctcg acaatagaac gtaacccacg agccccggtt ttgcggctaa tcgctttttg
     1261 ggcaattgaa attaatgcat ctttggtgaa gtgcaattca acgccttcca tttcgaataa
     1321 ggcttgatat tgcttgatga tcgcattttt cggctcggtt aagatttgaa tcagagcttc
     1381 ttcgtccaat tcttgcagcg gagtcaccac cggtaaacgc ccgattaatt caggaattaa
     1441 gccgaatttg actaaatctt ccggttcaac ttgtttgaat agctcggtta aatcttggcg
     1501 atctttatct tttttcaatg ccgctgcaaa accgatacca ccttgagtgt tggtacgggc
     1561 ttccacgatt ttatctaaac cggcaaaagc accgccgcaa ataaagagaa ttttggaggt
     1621 gtccaccgga atggtttcgc ctttcgggtg tttgcgaccg ccttgtgggt taatgttcgc
     1681 caccgtgcct tcaagtagtt ttaataaggc ttgttgaaca ccttcgcccg atacatcacg
     1741 ggtaatggat gcactttccg atttacgggt gattttgtcg atttcatcaa taaaaacaat
     1801 gcctcgttcc gcttgttcgg cttcaaaatc gcatttcatt aataattttt gaatcacgtt
     1861 ttcgacatct tctcccacat agcctgcttg ggtaagggta gtagcatcag ctaccgcaaa
     1921 aggtacgttt aaacggcggg caagggtttc tgccaacaag gttttaccgc taccggtcgg
     1981 cccgattaat aaaatattac ttttacctaa ctctacgcca ttagtggctt gatggtttga
     2041 caatgcactt cttaagcgtt tgtagtggtt ataaacggcc accgaaagca cttttttcgc
     2101 gtgttcttga ccgatgacat aatcatctaa atgagcgtgt aattcgtgag gtgtcggcac
     2161 attggtgaaa aaactgcctt catcgtcttg gttttcatca attaacggct cttcttcacc
     2221 gtgtaataag gcgtaggatt cctcgataca ttcattacaa atatagcctt ccgtgccttc
     2281 gactaattga tcgacttcgg tacgtttttt accgcaaaaa ctacagtggg gttctttttc
     2341 aaatgccatt acgccacctc actgcgagaa gttaatactt tgtcgattaa gccatactca
     2401 gcggcttctt ccgcagacat aaaattatca cgctcggtat cggctaacac tttttcatag
     2461 ctctgaccgc tgtgttctgc catacggcgg gtaagggtag ctttcaagcg taaaatttct
     2521 tccgcttgaa tttgaatatc ggttgcttga cctctcgctc cacctaaagg ttggtgaatc
     2581 ataatacgag cgtgcggcag agcaaagcgt ttaccttttg ccccggctga aagcaaaaat
     2641 gcccccattg aagccgcttg ccctgtgcaa agggttgcta catcgggttt gatgaagttc
     2701 attgtatcgt aaattgccaa gcctgctgtt accacgccac ccggtgagtt aatgtataaa
     2761 tagatgtctt tttccggatc ttcggcttct aaaaacagaa gttgtgccac aatcaggttt
     2821 gccatattgt cttccacctg accgtttaaa aaaataatac gttcttttaa taaacgtgaa
     2881 taaatgtcgt aagaacgttc gccttttgag gtctgttcca ccaccattgg gactaatgcc
     2941 attttgtttt cctctgtcgt aatacaaaat ctcgctattc tagcaaattt tcagaaaatc
     3001 attaacaaca agcggtcaga ttgttaaaat cttttgcaaa tgatgaggaa attatgacat
     3061 taacagaaat ttaccaaaaa tttgaacgtt gtaaatcgtg ggaagaaaga taccgcttgt
     3121 taattcagct aagtcgcttg ttaccaaaac caagtgagca agagttggca caaatagcgg
     3181 aaattgaggg ctgtgaaagc cgtttatggt ttgaatttca agctgagcca cgaaaagtag
     3241 tggcatatag cgatgcccgt ttaatgcagg ggattttatt tattgtgagt gtagctttaa
     3301 ctgaaaaaac ggcagaagag ctgaaaggct ttgacttaaa aacgatgttt gatgagctaa
     3361 aaatttcaca aaatttaacc agcacaaggt taaatgggtt gggtcagtta cagaaacata
     3421 taatgatgaa tttgtaattc accctcgccc cttgtgggag agggtgaata aaatccgcag
     3481 gattttattc gggagagggg aatataaaaa acctctgatt tctcagaggc ttaaatttgt
     3541 tggcggaacg gacgggactc gaacccgcga ccccctgcgt gacaggcagg tattctaacc
     3601 agctgaacta ccgctccata ctggtgctaa aataatagtg gtttcctcaa cattgagcaa
     3661 atagtttttg caaaatagtg tttaattggt tgtaaaataa ccactttatt tctgcttgat
     3721 cgccttgata aacagcaacg gcggacggtg ctgtaaatct ttaaattcat tgtgccaccg
     3781 aggttggcct gccagcattg gttcttccat ttgcacaatc tcaaacccgc cttgaataag
     3841 gttattcaca atggttgcca ttgtgcggtg gtaggtttta aagggttgtt gaaaccaact
     3901 tctgtcccgt tcgccctctt cccgataata gttcaagcgg taagcaactt gttgtttttc
     3961 ttggtttttc tcccaacgat agccttctcg gtaacaggtg acaatcgggt gttcttgcga
     4021 gaaaatcagc gtgccacaag cagtcatttt ttgcgaaatt tttgcaagta gttcggaaaa
     4081 atcagcaata taatgaaacg caaaagagct ggttactaca tcgaaatcgc cctcgctgat
     4141 ctcatttaat cgctccatcg gcaggcagta aagggaaaaa tcgttgcctt tttgccaatg
     4201 agcagaaaga tcgctttctg cctgttgtag cattaattca gataaatcca accccaccac
     4261 tttttctgca ccgagcgtta aataatgggc taaatgaact cctgtgccac aacctaaatc
     4321 cagcactttt ttgccggtta agtctggcag tagagagaac attgttggct tttccaccac
     4381 ttcattcata cttattggat tttgtcgaag ttgttggtaa cgctcaaaga aaatcggagt
     4441 gtcgtaaatg ctttttttca cttaatagcc tttaaacaaa tataaaataa ggcgggttaa
     4501 aacccgccac tacgatatga ttagtaagtc gataattttc gcaaagtttc taacgctttt
     4561 ccactctcaa gcgtttcttt tactttttgc acattggctt ttaaatcatc ttctccaaag
     4621 gttttcatta ataatgcgac atttgctgcc accgcatcaa tatgagccag tttacctttt
     4681 ccttgtaaaa tagcggttac tttttcggca ttttctgcag gttcaccgcc tttgagatct
     4741 tctaacgaat ggcgttcaat gccgaaatct tccggcgtta aggtgtaata gtcgatctgt
     4801 ccgttttgaa tttccgcaac ctgcgtttcg ccgtgaacgg caacttcatc taaacctgca
     4861 ccgtatacca caatagtgtg ttcgtaacct aaggctttta cggtttcagc atagactttc
     4921 actaactcag gggagtacac accgagtaat tggcgtttag cgtgagccgg gtttaccaac
     4981 ggccctaaaa tattgaaaat cgtgcgggtt tttaaggctt ggcgaacagg ggcggcatat
     5041 ttgaatccgc tgtggtaggt tggggcaaac aaaaagcaga tgttggtttc atctaaagca
     5101 aggcgtgccg tttcaggtga aacgtcaaca ttaataccta aatggcttaa tacatcgctt
     5161 gagccggttt tacttgatac gctacggttg ccgtgtttgg caattttata gcccattgtg
     5221 gcggcaacaa tcgcacttgt ggtagaaatg ttgatggtat tatgcccatc gccacctgtg
     5281 ccgacaatat ccgcaaaagg gtaatcaggc gttgagaacg gattggcatt ttcaagtgcg
     5341 gcttttgcag cacctgcaat ttcatcaacg gtttcgccac gcagttttag agcgattaag
     5401 gcagcagcaa gttgttcatt tgatacattg cctttaataa cttcactaaa aaaacggtga
     5461 gattccgctt gggctaaggc ttggttggcg aataatttat ctaaaagtgc ttgcatagtc
     5521 tcttcctttt taattagcga tgtgcctgat attccaaatc ggttgcctcg ttgccggtca
     5581 ttccacgaaa gccccagcaa tgctctggct gttctttaat ggtgatttca acgtccattg
     5641 gcacaatgcc gactttggtg tgtaaggcac taaatagtcc tttaattaag cgttttttga
     5701 gctgttcgct ccgcccttgc atcaggttta tttcaatcac aataaaatct tggctacggt
     5761 taattggggg atagaaattt tccgcatcca ataagtcaaa acgcaaagcg tggcgttgtt
     5821 ttggtacgcc aaggctcatt tgcatacagt caaaaatcac gtctgcaatc agggctctgc
     5881 ggtctgctaa ggtttgtttt aatccatata cactaatcat ttttttctcc taataaccat
     5941 tccaccgact gcttaagtaa ttttgtgcct tgcacggtta aaatggattc cgggtggaac
     6001 tggaaaccgc aaatcggtaa ggttttgtgg cgaattgcca tcacaatatg ttcaaatttc
     6061 gcattaacga taaattctgc cggtaattca tcgcccatta atgagtgata acgagcgaca
     6121 agcattggat tgttaatgcc tgaaaacatc gcttggttat cgtgttcaat acgagaaact
     6181 ttgccgtgca atactgtgcc ggtatgcact actttgccgc caaaggcctc aattagagct
     6241 tgatgtccta aacaaatgcc aatcatcggt actttgtctt tcagacggcg aataatttcc
     6301 aataaattgc ctgcctcagc cggattaccc gggccggggg aaagggcaac caaacaattt
     6361 tcggtggttt cagctttggc taaaaaatcc tcaagcggat aatcattgcg gaaaatcgtt
     6421 acctgatgcc ctaattcacg aaattgatcg accaagttat aggtaaaaga gtcgaaatta
     6481 tcaataaaaa gaatatttgc catagtttgt ccttatgctt ttgcattcac ctgcccaatc
     6541 gcttttaaca ccgcgcgtgc tttatggcgt gtttcatctg cctctaattg tggatcggaa
     6601 tccagcacct caccacaacc agcttgcaca taggcaatgc cattttgaac aaaggcggaa
     6661 cggatcacga tacaggtatc taaattgccg tctgaggtta aatagcccac cgcaccgccg
     6721 tagctatggc gtttttgttg ctcgacttgg tagatcagtt gcattgcctt aattttcgga
     6781 gcaccagtta gcgttcccat attcatacaa gcttgataag cgtgcagagc gtcaagatct
     6841 tttctaagtg tgccgatcac acgagaaact aagtgcataa tttgcgaata gcggtcaatt
     6901 tgcattaaat caaccacacg gcgagtgccg gattcgctca cacgagccac atcattgcgg
     6961 gcaagatcga ctaacattaa atgttcggca agctcttttt ggtctaaacg cagttctaat
     7021 tccaaacggg aatctaattc aggatcgata ttaccttgtt catcaaaacc gcgtgggcga
     7081 gagccggcaa tcgggtaaat ttccaactgg cggttggctt gcgaatattt caacgcactt
     7141 tcaggcgacg ccccaaacag agtgaaattt tcgccctgca tatagaacat atacgggctt
     7201 ggattgttga ttttgagctg tttatagctg gcaagagtat tcgggcaagg caagctgaaa
     7261 cggcgagagg gcacaatttg gaacacatca ccgatattaa tatggtgttt gagtttgcgg
     7321 ataatcgctt tgaaatcttc atcttcaata ttggcggttg cctcactcgt tgcggtttgg
     7381 ttcgctagag gcagctcaat atgtgacaga tttgcaagtt tttgcgtaaa ttcgaccgct
     7441 tgttggttca aattggtttg ttcattcggt tcaaaacaga aggtgtttag caccgcagtt
     7501 tcggtttggt ggtcgattgt aaccagttgc tctgctaaat agaaagtgta atctgggcaa
     7561 cttaagccat catcttgcag gctgatattt tccatcggaa taaaatttgc caccaaatcg
     7621 tagctaaata atccgcccaa atagataggc gatgtggaat cagcaaacag ttggctcacc
     7681 gttcttagcc cgtcaaacac ggtggtggtt ttgagcttgc tgtcttcatc taactggttg
     7741 ctgatggttg ggaaaatcgc ttgcaagcgg tcgtttttgt ctaaaaattt gcaagtcgga
     7801 gcagggttta acaggctcaa tgcgtttgca atcaaaggta aaaccgcttc tccgtttggg
     7861 gttaaagcgt ggaaagtcac ggtttgatct tcacagaaaa tatgcactgc cgatttcgca
     7921 aacagcaaac tttttacact gtttttactt tgaatttccg ctgaatctaa taatagcgta
     7981 tgtgggcgtt cgccgcataa tgcgtaataa acacgggtcg tatcggcaaa atagggtaat
     8041 tctgtttgag taatcgttaa ttgaggcata gcaaattcct attcaatttt tcgtactatt
     8101 aaactagtac attaaaaaga agtcaagcaa ttattccaga taattatgcg agcttgggcg
     8161 aacttggtgt ataattagcc caatttttag aaagtcgaag gcttgagact atgacacaat
     8221 ttttttatat ccaccccgac aacccacagc aacgcttaat taaccaagcg gtagagatta
     8281 ttcgaaacgg tggcgtaatt gtttacccta ccgattcagg ctatgctctc ggttgtgcaa
     8341 tgggcgataa gcacgcaatg gatagaattg tagcaattcg taaattgccg gaaaatcaca
     8401 attttacctt agtttgtagt gatttatccg agctttctat ctacgcgttg gtcaataatc
     8461 aagcctatcg cttaattaag aataataccc cgaatcctta tacttttatt ctgccggcaa
     8521 ctaaggaact tcctcgcagg ctgatgacaa agcgtaaaac agtggggatt cgtgtgccgg
     8581 ataatgcgat cgcattggcg ttaatttccg ccttaggtga gccgattctt tcttgctcgt
     8641 taatgttgcc ggagacggaa atgaccgagt cagaccctga tgaaattcgg gatcatttgc
     8701 aaaatcgggt ggatttaatt atccacggtg gttatttagg gcagcagcct acaacggtag
     8761 ttgacttaac cgatgacgtg ccggttatta ttcgagaagg atcgggcgat ccaacatcgt
     8821 ttaattaaat tgatgttgta tgattaatat atagaattat gcgtaggggg gctgtgccag
     8881 tccgtattaa tatcgttatt tggacaggca caggacctgt ccctacaatg aaaatcaaat
     8941 tcagacgctt gtgaaagcga caaaaggaac cttatgacaa cttttcaaaa aaaatcaact
     9001 gcgactttcc gtgcagacaa acgtggtgaa agccgtccct catttaaaaa agaagagcaa
     9061 aaatctgaac gcaaatttgg taaaacgtca gaaaaaccac cacgtccaac ttctaagcca
     9121 ttaacgcaaa gaattgcaaa aaaagtggaa aaaacgaccg ctagtgaagt ggcatctcaa
     9181 gagaaaaaag ccaaagtagt aggtgaaaag ctacaaaaaa tcttagcgcg agcagggcaa
     9241 ggttctcgcc gtgaattaga agagattatc gctgctggac gagtgagcgt ggacggtaaa
     9301 atggctactc ttggtgatcg tgtgcaggtt agctcatcga ctaaaatccg cattgacgga
     9361 catttagtta atttaatgcc ggcacaaaaa gagatctgcc gtgtattaat gtattacaaa
     9421 ccggaagggg aactttgcac ccgttccgat ccggaaggac gatccaccgt atttgaccgc
     9481 ttgcctcgtt taagtggcgc tcgttggatt gcggtcgggc ggttagatat taacacctca
     9541 ggcttattat tgttcactac cgatggcgaa ttagccaacc gtttaatgca cccaagccgt
     9601 gaagtcgaac gtgaatattc ggtgcgtgtg ttcggcaaca ttgacgatgc gatgttacaa
     9661 cgcttacgca aaggcgttca gttggaagac ggaccggcaa actttaaaca aattaaggtt
     9721 gtgggtggca caggcttgaa ccaatggttt gatgtgactt taaccgaagg gcgtaaccgt
     9781 gaggttcgcc gcctgtggga atcgcaaggg gtagaagtca gccgtttgat tcgtatccgt
     9841 tacggcaata tcaaattaga taaaggctta cctcgtggcg gctgggaaga aatgggctta
     9901 gaacaagtca attacttgcg tgagctggta gggttaccgc cggagactga aactaaagtg
     9961 gaagtggggg taaaccgccg ccgaaccaat atccgtcaaa ttcgtaaagc ggtaaaacaa
    10021 catcaaaaat atcgtggcta aacaccatct ttggggctgc attagttctg ctaatgcagc
    10081 tattttcttt caaaaaagga gcatttatgt cttgggatat tgttgcaaca ttggcggtca
    10141 ctgggattgc cgtattttta tttgcaactg aaaaaatgcg aatggacgta gttgcgattt
    10201 tagtgctgtg ttcattagta ttattagggc aggttgatac caaatctgct ctgagtggat
    10261 tttcaaactc agctactgta accgtaactg caatgtttgt attagctgct ggtttacaaa
    10321 atagtggtgc tttagatagt gtaggcagtt tacttgctaa ggtaaaatct ccttggttat
    10381 tcttgcttgt actctgtggc gtaaatgctg ctgtttctcc ttttgtgaat aatacggcag
    10441 tggtcgcagt attaattccg attgtgattg ccgcagcaca gaatataaaa atggctcctt
    10501 ccaaagccct tattccgtta tcattttctt ctcaaatggc aggtgtttgt acactgatcg
    10561 gcacttccac taatctatta gttaatgcaa tcgctcaaaa gcaagggcat tccggtttcg
    10621 gtatgtttga atttgcccct ttggggatta tttttttagt ggttggggtt gcctatttat
    10681 tagtgaccag ccgtttctta ttacccgaat cacatttgca acttgaagat ggtgaggggt
    10741 ttggaaaata tgtttctgaa cttaaagtaa ataaagactc tgtattaatt ggcaaaagta
    10801 catttgaatc aggattaaat gaagaattta atctatttac tattggtgtt ttgcgtgatg
    10861 gggaaagatt atcgacaccg agtcatcaag tattgcaaaa acatgatatt ttattgttgc
    10921 ggggtgaatc ggaaaattta gcaaaagtac gagaaaaata tggattgcac cacgttgtgt
    10981 atggacgccg tgagaatgat gaagataatc tggatgaaga cttgatggtg gctgaagtga
    11041 tgatttctcc tatttctcgt tggataggag ggactattcc tattttacaa cagcgttgga
    11101 ataaaaatgc aacggtgtta ggcattcaac gccaaagtaa agtaattcgt gagcgtttac
    11161 gcaccactgc ttttaaaatg ggggatattt tattactcac attaccaaaa gaggatatgg
    11221 agacgttacg ccaagataaa gactttattg tgttatcttc tgatttagtc aaaacggagg
    11281 aatcgtggaa aagtaaattt gctttagggg taatggtttt ggtggtggca acagcagcct
    11341 taggctgggt tcctatttca gtgagtgcct taattggtgc agttgcgatg tgtgttgctg
    11401 gttgtttaac ggctgaagaa gcctatcaat ctattgactg gaaaatcatt ttagtcttag
    11461 ctggattact gccattaggt gaagcgatgg caaatagtgg ggcggcgcaa tttattgttg
    11521 ataacacgct tggtaaagta ggggagttcg ggcctttagt ggtgttagct gtattatatt
    11581 tactgacaat ggtgttaacc gaatttatga gtaatgcagg gactgccgtg ttgcttacgc
    11641 ctattgcggt ttctaccgct aaaatgttag gggtagatgc ttccccattc attattgccg
    11701 taatgtttgc ggctgcgacc agctttatga cccctgttgg ctatcaaacc aacacgatgg
    11761 tatacggtgc aggtggctat aaattcacag attttattaa aattggttta ccgctaaatg
    11821 taatctattg gattttaggg atagtattaa ttccgatttt cttccctttt aatccataat
    11881 tgatagataa ttatagaaaa aaggtttaca gatcagaaac tctgtaaacc ttttttattc
    11941 agcaataaat caattttcta ttggaaaatt accattgata acccatgcca atactaccac
    12001 caaagtcgcc tctggtattg ctgttaccag ttaatttata gataaccttc ccgttatctg
    12061 aaatccgaga ataacctact gcgattgcag cttcattacg gtatgtatct ccagccgttg
    12121 ctaacatact tttacctggt agatatgctt gcggtaaacc tgcggttgca gttgcaccag
    12181 caataccagc gcgtaaacgt ttatcaactt tgtttacttt tgaatcaata ttattgatct
    12241 tattattgat atttttttct gttgctttta attggctaaa gttaactgca tcagtatctt
    12301 cagttcctgc ttttacgtta gtcacttttg ttcctgctgc attaatacct gttttagtta
    12361 tactcggtcc ccctacaata gttaatccat tagtatcaat ggttgtatca ccagtcttaa
    12421 cagtatcggc tacagcaatg ttagctgtta ctgtttgagc tgtgacagaa ttaactttaa
    12481 tatctttagc taatttaacc gttaatgaac cattaccatt tgaaataacc ccgatattat
    12541 tatcagttag ttgtgattcg ttagataccc ctccttttac tgtaacagta gaacctaatt
    12601 tacgttgaga tggcgtgccg gtatcacctg caaatgttag tgggctggtt actgcatcat
    12661 ttaacgcagt aattgcagag cctacattat tgaccgtttt actaacttca tttgtaggag
    12721 cgccatcaac cacagtatag cttggggctg aaacagcacc atttgcattt acacttgaat
    12781 taccacctaa agcggtagca atagtatttg ccgtatcgta aagttggcta ccattaactg
    12841 catctgctga gataggtgaa atatcaccat cagctacatt agttattttt aatttgccag
    12901 catcgacacc tgtcattgtg acacttgggc cattattaat ggttaagccg ttgttattta
    12961 gttttgaatt accaacagct acggagccgg caggagttaa atcaatgtct ttcgctaatt
    13021 taactgttaa tgtgttagca tctgttgcca ctacaccgat attattatca gtcaatatgg
    13081 ttgcatcggc cccaccttta acattcagag aagtacctaa tttacgaaca atttcagtac
    13141 cagcatcatc tacaaaagta agtgctccat tacttaactc attcactttc gtgtttaaac
    13201 catcaatttc ggtataagca gcataaagtt gtgaaccgtt gatagcatcc gttgatgagt
    13261 ccaaaatacg acctgctgca acattcgtta aagtacgttc attgcctaca gaaccaatac
    13321 ttacagttga gcttggcgta gtacctgcaa atgtgtatgt attgccattt agcgttgcac
    13381 tttttgttgc aactgtggct actgtttcag agttagtacc taatgcaaca gaaccttggt
    13441 gtgttgcttt tgcattttgg ccaagagcca ctgcagcagt tgttgttgct tgtgatgaaa
    13501 cacctaatgc aatagaagct acaccggctg catttgcact attacctaaa gcaatcgcag
    13561 agctacctgc agcattggca tttctaccca cagaagttga ccatattcca cctgcattag
    13621 cagagttacc taaagcggtt gactgtgtac ccgaagcatt tgcgttatag cctgcagcaa
    13681 gagaatctgc accgattgaa cttgcttcat aacctacagc ggttgaattt tcaccggtag
    13741 cttcagattt agagcctaat gcagttgcac gggtagctgt cgctgttgaa gctgtaccaa
    13801 tagctgttga ggtatcagca tttgcattgg ctgcagaatc aaccgcgagg gcatttgttg
    13861 catttgcatt cgcattgtag cctatagaag tggcattcat accatcagct gttgcgcgag
    13921 gaccaactgc agttgtacgt tcaccgtttg cttctgcttc agagcctaat gcggttgcca
    13981 gctcaccatt agcacttgtt gcaacgccga tagccattgg attaacaccg ctagagcctg
    14041 aattatcata gttagcacgt tgaatgccat catctgaaat actaacataa tgagttgcaa
    14101 atttgctgat agcttgtttg agttgactga cattgaccgc atcagtatct acatcgcctg
    14161 cggcaacatt ggcgatttta ttaccgccat tatctaagcc agatttagta agacttaccg
    14221 gtttgtctgc aacgccgtta gcgatggtga tacctgtatt gttaacaacg gtatcacctg
    14281 ttgttaatga accattagcg cctaagttaa ctttttctgc taattttatg gttaaagtac
    14341 tattgccatc agctacaaca ccaatattat tctcggttaa tatgcctgta gatccgcctt
    14401 tgatcttgac tgttgaacct agatggcgct caaaattagt tcctgtatca ccggcaaagg
    14461 ttaacggact gatcaccgct gtgtttaatg cgcttagtgc tgcagaaaca gtagtgtatc
    14521 tcccttgttt gccttcttta tctgcatctg gtgtgccatt cactaaagcg taggttggga
    14581 aagtgatatt tccattttca gtaacagctg ccccaccacc taagatattt gcggttgttg
    14641 cagcaacatt acctatagct tgttgtgtta aatataattg tgaaccgttt atggtatctg
    14701 tagagcttaa agatatacgt cctgcagcaa cattggtgat agttctctct ttgccttccg
    14761 ccccaatact tactgtagca attggatttg ttccagcaaa gtcactatat gtaataccat
    14821 ttacagttgc atttgtagtt ggtatggcta tagctgtaac tgagttgcta cctagcgcta
    14881 tcgaatcggt atgttcagca gtggcatttc gacctatagc ggttgcatta aaggcggaag
    14941 catttgatga ttctccgatt gcaattgtgc gttgatttgt tgctttagcg cttaccccta
    15001 gtgcaacaga agccgcgcct gatgcagtag cacgattgcc taaagcggtt gcagaacctc
    15061 cacttgcatt ggcttcacga ccaaatgcag aagcataatc gctggttgca cccgcattag
    15121 taccaacagc agtagttaaa tttgctgttg catttgcata ataacctaat gaggttgcac
    15181 cgtggcctga agtcttcgct tcataaccaa ttgctgttga gttatcgcca tttgcttgag
    15241 attttgcacc aattgctgtg gagtgaatag ctgttgcgtt agcttcagca ccataggcgg
    15301 agctaacaat accagttgca ttggcatgac tacctacagc agttgctttt cctattaccg
    15361 tttctcctgt tgctgctgta gttttgtaat tattaacacc atttgcaaga gcggaattac
    15421 cgatggcgat attatcatca ctggttacat ttgtagtagg tcctgctagt tttgcattag
    15481 cataagtacc aattgcaata ttgtggctac cactagtacc gtctccagca gtattaccaa
    15541 tagaaatcgt attgtttcct gttacattag agctagccga ataaccaata ccaatattgt
    15601 attctgtgct gttagagtga tttaatccgg catgatcgcc aataaaaact tcacgtaagc
    15661 ccattgcggt tgcattgtac cctactgcta cggaacgttt atcggcagtg gcttgcccac
    15721 caacagctgt tgaagcaaaa ctagcgttag caaagcctcc taatgcagtt gaaaaatcgg
    15781 ctgtcgcggt tgtgttatac cctaatgctg tagaaacttt accgctattt ccttctaagc
    15841 ctgttattgt gcttactagt gttgagttaa ctttagatcc atcaccaatt gcgatagatg
    15901 tttgtgggtt attatctggt atggtcccat aggttggtga ataggttgca gcacttgcaa
    15961 aacctgcaga aagcgcaaga cttagtgtgc tgagtttaat aatattagtg gttagtgatg
    16021 tatttgttgc attagattta ttagcacttc ttgataattc agaagcaaca atccaagttc
    16081 gttttgcatg gttccaaaca atgcg
//
LOCUS       DANLYI010000039        15948 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.039, whole genome shotgun sequence.
ACCESSION   DANLYI010000039 DANLYI010000000
VERSION     DANLYI010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 15948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15948
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.039"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            33..2255
                     /gene="glgB"
                     /locus_tag="PXD81_000899"
     CDS             33..2255
                     /gene="glgB"
                     /locus_tag="PXD81_000899"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HDL5536947.1"
                     /translation="MQDYTYSEKDAFFVERLSSGSCNDPFSYLGIHPSNNGVIIRVFL
                     PSAIACQVIDLNGKPVSSMLKIDDRGFFIAELIGKKTDFSYRLLVEYPDSTVDIEDPY
                     RFKTHIQELDNWLFAEGKQLRPYEKLGAHLVTQNEVGGVHFSVWAPNAQRVSVVGDFN
                     HWNGLVHSMRFHPDSGIWDIFLPNVKKGECYKFEVLDRNGVLRLKSDPYAFASQFRPE
                     TASIVSGLPSIVPAEKTCRNANAPDKPISIYEVHLGSWRRNIENNYWLSYEELADELI
                     PYVKDMGFTHIELLPISEFPFDGSWGYQTTGIYSPTSRFGTPEGLSYFIQKAHDKGIG
                     VILDWVVGHFPVDEHGLVYFDGTHLYEHSDPKEGYHKDWNTLIFSYGRNEVSNYLSGN
                     ALYWIERFGIDALRVDAVASMIYRDYSRSDGEWIPNKYGGRENLEAIDFLRHTNNILR
                     DESQGGASIAEESTSFAGVSHSTERGGLGFNYKWNMGWMNDTLRYMQKDPIYRRYHHN
                     LMTFGMTYQYSEQFVLPLSHDEVVHGKGSLLNKMPGDCWQKFANLRAYYGYMWGYPGK
                     KLLFMGNEFAQGKEWNFAESLDWYLLGEEGGEWHKGVLGWVKALNHLYTQTPALFEQD
                     YNPEGFEWLVVDDHDNSVFAFERKAKNGDSVIVVSNFTPIVRENYRFGVNELAEYQEI
                     LNSDLACYCGSNVSNANRFASELIASHGKAYSLSITLPPLSTIFLKRAADKKTKQNKT
                     "
     gene            2279..4291
                     /gene="glgX"
                     /locus_tag="PXD81_000900"
     CDS             2279..4291
                     /gene="glgX"
                     /locus_tag="PXD81_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HDL5536948.1"
                     /translation="MHYFRGKPFPLGNKLDRQAGEFGTNFAIVSQNATAIELCLFDEN
                     HHETRIPMFSSGDTWHIFVAGVQAGTKYGYRVKGIQDEQAGLLFNPQKLLTDPYAKAI
                     EGKPDLSSAEAAAWYKWNDERDNASLAPKSIVIDEHFDWENDSQLFTPWAETIIYEMQ
                     VKGFSQRNFQIPSDIRGTFAGLAHPVSIDYLKTLGITAVELLPVTYHIDETHLQKQGL
                     VNYWGYNVLGNFAIDPNLAADKQNPLKEFKQLVKTLHQAHIEVIMDIVFNHTAESDKN
                     GPMLSFRGIDNTAYWLDKQGEYENWTGCGNTLNLSNPYILCWAIDCLIYWVEECHIDG
                     FRFDLASILGRTPSFSEKADFFTALKSHSKLTHIKLIAEPWDIGVEGYQLGQFPEPFY
                     QWNDCYRDDIRRFWLQNQGDWGLLARRLAGSDDVFTKYSVSKSINFITAHDGFTLQDL
                     TSYQQKHNWANGEQNRDGHNSNYSNNHGVEGQTADELVKKQREITACSLLATLFLSAG
                     VPMLLAGDEIGHSQQGNNNSYCQDNEITWLDWENMNHTRLDYVRSLIRLRKKIALLSK
                     QNNWWQEDSVQWLNPSAQLMNIDDWHNQHNGLVVLLQEEWLLLFNANRQPQPFLLPQG
                     NWKVVLEQAQGILMTDTQAVTLNNMGVCVLQKSDVMFANKLETQYA"
     gene            4284..5597
                     /gene="glgC"
                     /locus_tag="PXD81_000901"
     CDS             4284..5597
                     /gene="glgC"
                     /locus_tag="PXD81_000901"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HDL5536949.1"
                     /translation="MLNQESTALVARQDLTNDTLVLILAGGRGSRLYELTDKRAKPAV
                     YFGGSRRIIDFALSNCINSNLLKIGVITQYAAHSLLRHLQRGWSFLPYERNQYIDMLP
                     ARQQLDENTWYRGTADAVYQNMAMMKSHYRPKYVVILAGDHIYKMDYTQMLKDHVDQG
                     AKCTVGCIEVPRSQATEFGVMAVNEKLKVKEFVEKPADPPAMPGKPDSSLASMGIYVF
                     NADYLYEMLEKEASTPGTSHDFGKDIIPKAVEQGVLYAHPFERSCKGRNASGAIYWRD
                     VGTIDSYWAAHMDLVSEDPELDLYDESWPIHGRPQQTAPARFFYNRARARTLDNSLIA
                     GGCVITDAEISNSVLFNRVHVEAESVIEHTVILPQVTIGKNCVIKRAVIDRHCVIPDG
                     LEIGVNAEEDAKRFRVSKNGIVLVTQSMLDKLAGKEANSDIKYTI"
     gene            5720..7156
                     /gene="glgA"
                     /locus_tag="PXD81_000902"
     CDS             5720..7156
                     /gene="glgA"
                     /locus_tag="PXD81_000902"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017357702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HDL5536950.1"
                     /translation="MKVLHICSEMYPLIKTGGLADVMGALPFAQQQAGNDVRVLIPYY
                     PKVAQTLGETVEVATIGTFAGTVSVRFAYFNGLGVYVIDAPHLFMRDGNPYHDSNYQD
                     YPDNYKRFALLGYIGAQLAQGLDHWWGTAQVLHAHDWQAGLACAYLKSWGSNVKTLFT
                     IHNIAYIGRFNAFHLAELGLPWEFFHAEGLEFYGEISYLKAGLYFADRITTVSPTYAR
                     EITEEIAGAGLHGLLATRQYQGRLRGILNGVDAEVWNPETDTHIVVNYRANYMHGKAK
                     NKAELQRIFGLPEDKEALLMVMVTRLTEQKGADFLLANIDTLMQHKVQIIVLGSGSPD
                     LENALRYFQHKYPNQIGVKIGYDEALSHQIIAGGDLILVPSRFEPCGLTQLYGLKYGT
                     LPLVRRTGGLADTVVDSHKESIENRSATGFVFDYPDAPAFLEAAMRAISLWQKQKLWS
                     SVRQNALAQDFGWAKVAEQYQNLYHEII"
     gene            7251..9751
                     /locus_tag="PXD81_000903"
                     /pseudo
     CDS             7251..9751
                     /locus_tag="PXD81_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651737.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
     gene            complement(9838..10554)
                     /gene="trmJ"
                     /locus_tag="PXD81_000904"
     CDS             complement(9838..10554)
                     /gene="trmJ"
                     /locus_tag="PXD81_000904"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HDL5536951.1"
                     /translation="MQILNQIQIILVETSLPANIGSAARAMKTMGLTHLRLVAPKQPL
                     DEQAEALAAGAKDVLDNAQCYATFDEAIADCQLVIGTSARLRHLQNTLIEPRDCGELV
                     VNRTNLGKVAIVFGRERVGLTNEELLKCQYHLNFPTNPDYGSLNLAMAVQLASYEIRM
                     AYLQNSGEKQPLAADKDYPTAQALEHFFAHTERLYTELGFIRNDSVMLKLRRLYQRAE
                     LETNELNLLRGMLTAVEKAN"
     gene            10734..12272
                     /gene="pntA"
                     /locus_tag="PXD81_000905"
     CDS             10734..12272
                     /gene="pntA"
                     /locus_tag="PXD81_000905"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007525314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HDL5536952.1"
                     /translation="MLIGVPRELLDGETRVAATPKTVEQIKKLGFDVLVEQDAGFKAS
                     FEDNTFLNAGANIGTQQEVWNADIIFKVNAPTEAEIELIKEGVTLVSFIWPAQNPQLM
                     EKLQSKKINVLAMDAVPRISRAQALDALSSMANISGYRAVIEAANAFGSFFTGQITAA
                     GKVPPAKVLVIGAGVAGLAAIGAANSLGAIVRAFDSRPEVKEQVKSMGADFLEIDFEE
                     EGGSGDGYAKVMSEEFNRRAMALYAEQAKEVDIIITTAAIPGKPAPRLITKEMVDSMK
                     PGSVIVDLAAATGGNCEYTKAGEIVVTDNQVKVIGYTDFPARLPTQSSQLYGTNLVNL
                     LKLLAPNKDGQIEINFDDVVLRGVTVVRDGELTWPAPPIQVSAQLQKQAAAPQAVKKE
                     EKPADPRVKYGVMAAAGVLFLWLASVAPAAFLSHFTVFVLACVVGYYVVWNVSHALHT
                     PLMAVTNAISGIIIVGAVLQIAQPTGNFFIDILAFIAILVASINIFGGFKVTQRMLAM
                     FRKG"
     gene            12287..13732
                     /gene="pntB"
                     /locus_tag="PXD81_000906"
     CDS             12287..13732
                     /gene="pntB"
                     /locus_tag="PXD81_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005597319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HDL5536953.1"
                     /translation="MSFGFVTAAYIIAAILFIMSLAGLSKHETAKAGCWYGIVGMAIA
                     LVATIFGPQSHGTIWILIAMAIGGFIGVKKALKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYGLHTNALMPANLDETAQAAFLAEQATLANIHNVEVFLGIFIGAVTFSGSLVAFG
                     KLSGKLFGRKVSSAALNIPHKHKWNLAAIIVSVFLMIVFLNNPHNIFPVLIMTIIALV
                     FGWHLVSSIGGADMPVVVSMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYI
                     MCKAMNRSFISVIAGGFGNEVQSGKGDEEYGEHRETKAEEVAEMLKNASSVIITPGYG
                     MAVAQAQYPVAEITAKLREKGVNVRFGIHPVAGRLPGHMNVLLAEAKVPYDVVLEMDE
                     INDDFEETDVVLVIGANDTVNPAALDDPSSPIAGMPVLEVWKAQNVIVFKRSMAVGYA
                     GVQNPLFFKENTQMLFGDAKERVDDILRALN"
     gene            complement(13799..14725)
                     /locus_tag="PXD81_000907"
     CDS             complement(13799..14725)
                     /locus_tag="PXD81_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005598065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDL5536954.1"
                     /translation="MKPIFLEIRHLRTLIALKESGSVSLAAKRVHLTQSALSHQIKLL
                     EEQYDLTLFERKSSPIRFTPAGERLIKLAYDILPKVIEAELDLARVKQGEVGEVRVAV
                     ECHTCFDWLMPAMDTFRQHWPLVELDIVSGFHTDTVGLLLSHRADLAIVAEEEDSAGI
                     IYKPLFSYEMVAICSKDHPLATKDVWEAEDFKDETLITYPVPDDMLDLLRKILGPAGI
                     KVTRRNSELTIAIIQLVASRRGIAALPYWAVKPYLDRGYVVARKITQQGLYSTLYGAI
                     READATLAYLDDFHDTVKSQSFATLPGLMVLE"
     gene            complement(14835..>15948)
                     /gene="asd"
                     /locus_tag="PXD81_000908"
     CDS             complement(14835..>15948)
                     /gene="asd"
                     /locus_tag="PXD81_000908"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017357680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HDL5536955.1"
                     /translation="QNVGFIGWRGMVGSVLMDRMVEENNFANINPVFFTTSQAGQKAP
                     VFGGKDAGELKNAFDIEELKKLDIIVTCQGGDYTNEVYPKLKETGWNGYWIDAASALR
                     MKDDAIIVLDPVNQHVISEGLKNGIKTFVGGNCTVSLMLMAIGGLFEKDLVEWVSVAT
                     YQAASGAGAKNMRELLSQMGELEDSVKAELANPASSILDIERKVTAKMRAEDFPTENF
                     GAALGGSLIPWIDKLLPETGQTKEEWKGYAETNKILGLSDNPIPVDGLCVRIGALRCH
                     SQAFTIKLKKDIPLAEIEQIIAAHNEWVKVIPNDKETTLRELTPAKVTGTLSVPVGRL
                     RKLAMGGEYLAAFTVGDQLLWGAAEPVRRILVQLTQ"
BASE COUNT         4565 a         3097 c         3655 g         4631 t
ORIGIN      
        1 taagtattaa acgctttaag caaggagtgc cgatgcaaga ctatacttat tctgaaaaag
       61 atgctttttt tgttgagcga ctttcttcgg gttcttgtaa tgatcctttc tcctacctag
      121 gtattcaccc tagcaataac ggcgttatta ttagggtatt tcttccttct gccatagctt
      181 gccaagtgat cgatttaaac ggaaagcctg tgtcatcaat gctaaaaatt gatgatagag
      241 gcttttttat tgcagagctg attggtaaaa aaacagattt ttcttaccgc ttgttggtgg
      301 aataccctga ttcaacggtg gatatagaag acccttaccg ttttaaaacc catattcaag
      361 agcttgataa ttggttgttt gcggaaggaa aacaacttcg cccgtacgaa aaattaggcg
      421 cacatttagt cacacaaaat gaagttgggg gtgtgcattt tagcgtctgg gcgccgaacg
      481 ctcagcgggt ttccgtggtt ggggatttca accactggaa cggcttggta cattcaatgc
      541 gattccaccc cgattccggt atttgggata ttttcctgcc gaatgtgaaa aaaggggagt
      601 gctataaatt tgaagtgttg gatcgaaacg gcgtgctacg gttgaaatcc gacccttacg
      661 cctttgcctc ccaattccgc ccagaaaccg catctattgt ttccggcttg ccaagcattg
      721 tgccagccga gaaaacctgc cgtaatgcga atgctcccga taaaccgatt tccatttatg
      781 aagtgcattt aggctcttgg cgacgcaaca ttgaaaacaa ttattggctt agctatgaag
      841 agcttgccga tgaactcatt ccttatgtga aagatatggg ctttacccat attgaattgc
      901 tgcctatttc cgaatttccg tttgatggct cttggggtta tcaaaccacc ggtatttatt
      961 cgccaaccag ccgttttggt acgcctgagg gcttgagtta ctttatccaa aaagctcacg
     1021 ataaaggcat tggtgtgatt cttgactggg tagtggggca ttttccggta gatgaacacg
     1081 gcttggttta ttttgatgga acgcatttat acgaacattc cgatccgaaa gagggctacc
     1141 acaaagattg gaatacctta atttttagct acggcagaaa tgaggtgagc aattatttgt
     1201 ctggtaatgc actttattgg attgagcgtt ttgggattga tgcgttgcgg gtggatgcgg
     1261 tggcgtcaat gatttatcgt gattactcac gttcagacgg cgagtggatt ccaaataaat
     1321 atggcggacg tgagaactta gaggcaatcg acttcttacg ccacaccaat aatattttga
     1381 gagacgaaag ccaaggcggt gcgagcattg cagaggagtc cacttctttt gccggagtgt
     1441 ctcactcaac tgaaagaggc ggtttgggct ttaattataa atggaatatg ggctggatga
     1501 acgacacttt acgctatatg caaaaagatc caatttaccg ccgttatcac cacaatttaa
     1561 tgacttttgg tatgacctac caatacagcg aacagtttgt gttaccgctt tcacacgatg
     1621 aagttgttca cggcaaaggt tcattgctga ataaaatgcc gggggattgc tggcaaaaat
     1681 ttgcgaactt acgagcctat tatggctata tgtggggcta tccgggcaaa aaattgctct
     1741 ttatgggcaa cgagtttgct caaggtaaag agtggaattt tgccgagagc ttagattggt
     1801 atttactcgg ggaagaaggg ggcgaatggc ataagggcgt attgggttgg gtaaaagccc
     1861 ttaaccattt atacacccaa acccctgcac tgtttgagca agattacaat cctgaaggtt
     1921 ttgagtggtt agtggtggac gatcacgata attcggtgtt tgcttttgag cgtaaagcca
     1981 aaaatggcga ttcggtgatt gtggtgagta atttcacccc gattgtgcgt gaaaattacc
     2041 gttttggggt gaatgaattg gcagaatatc aggaaattct caattctgat ttagcttgtt
     2101 attgcggtag taatgtcagc aatgcaaacc gatttgccag cgagttaatt gcatcgcacg
     2161 gtaaagccta ctccttgagc attactttgc caccgctatc gactatcttc ctcaaaagag
     2221 ccgcagacaa aaaaacaaaa caaaacaaaa cataacttaa agaaacataa aaaataatat
     2281 gcactatttt agaggtaagc cttttccttt aggcaataaa cttgatagac aagcgggtga
     2341 atttggcaca aattttgcca ttgtttcgca aaatgcaacc gctattgagt tatgtctttt
     2401 tgatgaaaat caccacgaaa cccgtattcc gatgttttct tccggagata cttggcacat
     2461 ttttgtagca ggtgtacaag cagggacaaa atatggctac agggtgaaag gcatacaaga
     2521 tgaacaagcc ggtttgttat ttaatccgca aaaattattg actgatcctt acgctaaagc
     2581 gattgaaggg aagccggatt taagtagtgc agaggcagcc gcttggtata aatggaacga
     2641 tgaacgggat aatgcctctc ttgcacctaa gtctatcgtg attgatgagc attttgattg
     2701 ggaaaatgat agccagttat tcactccttg ggcagagact attatttatg aaatgcaggt
     2761 gaaaggtttt agtcagcgga actttcagat tccaagcgac attcgaggca cttttgccgg
     2821 tttggctcac cctgtttcga tagactattt aaaaacctta ggaatcaccg cagtagaact
     2881 tctacccgtt acttaccata ttgatgaaac ccatttgcag aaacaagggc ttgtgaatta
     2941 ttggggctat aatgtattag gtaattttgc tattgatccg aatttagcag cagataagca
     3001 aaatcctctt aaagagttta aacagctcgt aaaaacatta catcaagcac atatcgaagt
     3061 gattatggat attgtgttta accacacagc tgagagtgat aaaaacgggc cgatgctaag
     3121 ttttagaggc attgacaata cagcttattg gctagataaa cagggcgaat acgaaaattg
     3181 gactggctgt ggtaatacct taaatttgag caatccttat atactctgct gggcaatcga
     3241 ttgcttaatc tattgggtgg aggagtgcca tattgatgga ttccgctttg atcttgcctc
     3301 aattctaggt agaacaccga gtttttctga aaaagcagat tttttcacag cacttaaaag
     3361 ccattctaaa ttaacacata ttaagttgat tgctgagcct tgggatattg gtgtagaagg
     3421 ttatcaatta gggcaattcc ctgagccttt ttatcagtgg aatgattgtt atcgtgatga
     3481 tatacgccgt ttttggttac aaaaccaagg tgattggggg cttttggcac ggcgattggc
     3541 aggaagtgat gatgtgttta ctaaatattc cgtttcaaaa agtatcaatt ttattacggc
     3601 acacgatggg tttacgttac aagatctcac cagctatcag caaaaacata attgggcaaa
     3661 tggtgaacaa aaccgagacg ggcataatag taactacagc aataaccacg gtgttgaagg
     3721 gcagacagca gatgaattgg taaaaaaaca gagagaaatc accgcttgta gtttgcttgc
     3781 tacgctcttt ttatcggctg gtgttccaat gcttttagcc ggtgatgaaa tcggacatag
     3841 tcaacaaggt aacaataata gttattgcca agataatgag atcacttggc tagattggga
     3901 aaatatgaat cacacacggc tggattatgt tcgtagttta atccggttgc gtaaaaaaat
     3961 cgctttgctt tccaagcaga ataattggtg gcaagaggat tcggtacaat ggttgaatcc
     4021 ttctgctcag ctaatgaata ttgacgattg gcataatcaa cataatgggc ttgtcgtttt
     4081 attacaggaa gaatggctgc ttttattcaa tgctaatcga caacctcaac cgtttttact
     4141 gccacaaggc aactggaaag ttgtgttgga gcaggctcaa ggtattttga tgacagacac
     4201 gcaagcggtt acgctcaata atatgggtgt atgcgtactt caaaaaagtg atgtaatgtt
     4261 cgctaataaa ttggagacac aatatgctta accaagaatc aacggctctt gttgcaagac
     4321 aagatttaac caacgatacc ttagtattaa ttcttgcagg aggacgaggt tctcgcctat
     4381 acgaattaac cgataaacgt gcaaaaccgg cagtctattt cggtggtagc cgccgtatta
     4441 ttgattttgc gttatcaaat tgtattaact cgaatttatt aaaaatcggg gtgattaccc
     4501 aatatgcagc acactctttg ttgcgccatt tacaacgtgg ttggtcattt ttaccttatg
     4561 aacgtaacca atatattgat atgttaccgg cacgccaaca gctagatgaa aacacttggt
     4621 atcgtggcac agcggatgcg gtgtatcaaa atatggcgat gatgaaatcg cactatagac
     4681 caaaatatgt ggtgatttta gcaggggatc atatctataa aatggactac actcaaatgc
     4741 tgaaagatca cgtcgatcaa ggtgcaaaat gtactgtagg ttgtatcgaa gtgcctcgct
     4801 cgcaagcaac agagtttggg gtaatggcgg tgaatgaaaa gctgaaagtg aaagagttcg
     4861 tcgaaaaacc agctgatcca cctgcaatgc cgggaaaacc ggattcctcg ctagcatcaa
     4921 tggggattta tgtatttaat gcagattatc tatatgaaat gctggaaaaa gaagcctcta
     4981 caccgggaac ctcacatgat ttcggtaaag atattattcc aaaagcagta gagcaaggtg
     5041 tgctatatgc ccacccattt gaacgttctt gtaaagggcg taatgccagt ggggcaattt
     5101 actggcgaga tgtcggtacg attgacagct actgggctgc ccatatggat ttagtttctg
     5161 aagatccgga attagattta tatgatgaat cttggccgat tcacggtcgt ccgcaacaaa
     5221 ctgcaccggc acgttttttc tataatcgtg cgcgtgccag aaccctagat aactcactga
     5281 ttgcgggtgg ttgtgtgatt accgatgcgg aaatcagtaa ttcggtctta tttaaccgtg
     5341 ttcacgttga agcggagtcg gtgattgaac acacagtgat tttaccgcag gtgactatcg
     5401 gtaaaaattg cgtaattaag cgcgctgtga ttgacagaca ttgtgtaatt cctgatggct
     5461 tagaaattgg tgtaaatgcc gaagaagatg cgaaacgttt tcgtgtcagc aaaaatggca
     5521 ttgtattagt gacacagtca atgttagata aattagcggg aaaagaagct aattcggata
     5581 ttaaatatac aatttagtaa gagatgatat aatgcttcga gtttgtcatt tgaggtcgca
     5641 agctcgaagt attgttatga ttaaagcgct tcaagcggtc ttatttttca actattttac
     5701 acatagaaaa cggttaaaaa tgaaagtttt acatatttgc tctgaaatgt accctttaat
     5761 taaaacaggc ggtttagctg atgtaatggg ggcattgcca tttgcgcaac agcaagctgg
     5821 taatgatgtc agagtgttaa ttccttatta cccgaaagtc gcgcaaactt taggggaaac
     5881 ggtagaagtt gctactatcg gcacttttgc cggaacagtc tctgttcgat ttgcttattt
     5941 taatggctta ggcgtttacg ttatcgatgc gcctcattta tttatgcgtg atggtaaccc
     6001 ttaccacgac agcaattatc aagactatcc ggataactat aaacgctttg ctttattagg
     6061 ttacattggg gcgcaattag cccaaggttt ggatcattgg tggggaaccg ctcaagtttt
     6121 acacgcacac gattggcaag cagggcttgc ttgtgcatat cttaaaagtt ggggaagcaa
     6181 tgtaaaaacg ctcttcacta ttcataatat tgcctatatc gggcgcttta atgccttcca
     6241 tttagcagag cttggcttac cttgggaatt tttccacgcc gaaggcttag aattttacgg
     6301 tgaaatttct tacctcaaag caggtttgta ttttgctgat cgcattacta ccgttagccc
     6361 gacctatgct cgtgaaatta ctgaggaaat tgccggtgca ggcttgcatg gtttactggc
     6421 aacccgtcaa tatcaaggta gattgcgtgg cattttaaac ggggtcgatg ctgaggtgtg
     6481 gaatccggaa acggatacac atattgtggt gaattatcgt gcgaactata tgcacggtaa
     6541 agccaaaaat aaagcggaac tccaacgaat ttttggtttg ccagaagaca aagaagcatt
     6601 attgatggta atggtgactc gtttaactga gcagaagggg gcagatttct tactcgccaa
     6661 tatagatacc ttaatgcaac ataaggtaca gattattgta ttgggaagtg gatcgccaga
     6721 tttagaaaat gcgctacgtt atttccaaca caaatacccg aaccaaattg gggtgaaaat
     6781 cggttatgac gaggctttat cgcaccaaat tattgcaggt ggtgatctga ttctagtacc
     6841 aagccgtttt gagccgtgcg gattaactca actttatggg ctgaaatatg gcactttacc
     6901 gctggttcgc cgtaccggtg gcttagctga tacggtggta gatagccata aagagagcat
     6961 tgaaaaccgc agtgccaccg gatttgtatt tgattaccca gatgcacctg cgtttttaga
     7021 ggcagctatg cgggcaatca gcttatggca gaaacaaaaa ttgtggtcga gcgttcgcca
     7081 gaatgccctt gctcaagatt ttggttgggc aaaagtggca gaacagtacc aaaatttata
     7141 ccacgaaatt atttaatttg tattcacaac atcatttcaa gcggtcgaat ttgcggattt
     7201 ttttgtaaaa acgaccgctt gcaatattaa aaattaggag aatttactca atgagcaggc
     7261 agtcattaag cccagaaatg atcaaaaatg ttaaaaatgc gattttgcac aaaatggtgt
     7321 tcgctttagg ggtcgaaccc cgtgatgcca gtaagcgtaa ctggctcaat gcaaccttac
     7381 gtgtagtgcg tgatttatct accgaagatt ggttacaaac ccgccgtagc caggttgcaa
     7441 atggtagccg ccgtgtttat tacctttcaa tggaattttt aatggggcgt acgttcagta
     7501 acgcaatgat ttctgaaggt gtgtatgagc ttattggcgc tgcgttaaaa gagttagggc
     7561 aagatctgga agagattatc aatgaagagg gcgtccgggc ttaggtaacg gtggcttagg
     7621 gcgtttagcc gcctgctata tggacagcct tgccacaatg aaaattccgg caatcggcta
     7681 cggtattcaa tatgaatacg gtatgttccg ccaagaaatc cgcaacgggg agcaaattga
     7741 gcaacctgat gaatggttag agaatgaatt tgtgtggcca tatctacgtt caagtaagcg
     7801 tttcccggta cgctttggcg gtaaaacttg gcgtgagggc aaaaaagtag tatggcagcc
     7861 ggaagaagaa attaccgctc aagctcacga ccaactgatc ccgggctttg agaccacctc
     7921 aactaacagc ttacgtttat ggtctgcaca cgcatcgggt agagggtttg gcttatctga
     7981 ttttaaccgt ggcgattatt ttgcagcaat ggttaaacaa aacagctcgg aaaacgtctc
     8041 tcgtgtgctt tatccggacg attctaccta taacggtcgt gaattacgct tacgccaaga
     8101 gtatttcctt tgctcggcat ctgtacaaga tattatccgc cgccacgaag cggagttcgg
     8161 ctcttgtgtg aatttagccg acaaagtggc aactcactta aatgacacac acccaaccct
     8221 tgcggtgcct gagttaatgc gtattttaat tgacgaaaaa ggctatggct gggaacaagc
     8281 gtgggcgatg actcgtaaaa ttttcttcta caccaatcac actttgatga gtgaagcgtt
     8341 agaaacttgg ccggtagaaa tgttagcccg tgtgctacca cgccatttag agattatttt
     8401 cgacattaac gaatatttct tagaggaagt gcgtagccaa ttcccggaag atcacgattt
     8461 gatttctcgt gtttccttaa ttgatgaaca aggcgatcgc cgtgtgcgta tggcgtggct
     8521 tgcggtagtc gcctcagcga aagtaaatgg tgtggcgaaa attcactcgg atttaatggt
     8581 tgaatctatt tttgccgatt ttgcccgtat ttacccaaat cgttttacta atgtgactaa
     8641 tggggtgaca cctcgccgct ggatcaaaat tgccaaccca gggcttgcta atattttaga
     8701 taaatgtatc ggcaataagt ggctgactga tttaagtgaa ttggaaaaat tcaacgtgtt
     8761 tgttgatgat gctgatgtgc aagcacaggt ggcagcggtg aaagtagaaa acaaacgtcg
     8821 cttagccaaa tatgtcgaag aaactcaggg aattaaactt aatcctgaag cgatttttga
     8881 tgtacaagtt aagcgtattc acaagtataa acgccagcaa atgaacgtgt tgcatatcat
     8941 cacgctttat aaccgcatct tgaaaaaccc gactgctgat tggacacctc gtgtgtttat
     9001 cttcgcaggg aaagcggcaa gtgcttatta tgcggcgaaa aaagtgattc gtttgattaa
     9061 tgatgtggca aatgtggtga ataatgaccc tcgcattaat gatttaatta aggtggtgtt
     9121 tattccgaac tacggtgttt cattagcaca aatgattatt ccggcagctg atgtttcaga
     9181 gcaaatttcc ttagcgggta cagaggcctc cggaacctct aatatgaaat ttgccctcaa
     9241 tggtgcttta accattggta cattagacgg tgcgaatgtc gaaattttag atcacgttgg
     9301 taaagagaac atctttattt tcggtaatga agtagagcag gtggaagagc ttcgccacaa
     9361 cggttactca ccgtatcact actacgaaaa agacagcgaa ttgaatgagg caatttcaca
     9421 aattctaaac ggtaaattct caccggaaga tccgtatcgc tatcaagatt tgatcttaaa
     9481 ttcaggtgac tactaccaag cctgtgccga ttatcgtagc tatgtggagg ctcaagaaaa
     9541 agtggctcaa gcctatcgta acaagaaagc ttggacacgt tcagccatca tcaatattgc
     9601 caatatgggc tatttctcct cagaccgctc ggtgatggat tatgcgaaaa atatttggca
     9661 tatcgaaccg atgagcgaag aacaattaaa aggcgattct acaatgggca gtattattga
     9721 aagttcaaat aaactgttga atttaaaata atgatttaga gaaaacggta agagtaatct
     9781 taccgttttt gttttagtca gggattttgc aaatttctac taaaaattaa ccgcttgtca
     9841 atttgccttc tccaccgccg tgagcattcc cctcagtaaa ttcagctcat tagtttccag
     9901 ttcggcacgt tggtataaac ggcggagttt tagcatcact gaatcattgc ggataaagcc
     9961 taattcggta tataagcgtt cggtgtgagc aaagaaatgc tctaaggctt gagcggttgg
    10021 gtaatcctta tctgctgcaa gcggttgttt ttcgccagaa ttttgcaaat aagccattcg
    10081 gatttcataa cttgcgagct gtactgccat tgccaaattc agagaaccat aatcaggatt
    10141 ggttgggaaa ttcaaatggt actggcattt caataattct tcgttggtta gccccactcg
    10201 ctcacgtcca aatacaatcg ccactttgcc aagattggtt cgatttacca ctaattcacc
    10261 acagtctctt ggttcaatca gggtattttg caaatggcga agtctggcac tggtgccgat
    10321 caccagttgg caatcggcaa tggcttcatc gaaagtggcg tagcattgag cgttatccaa
    10381 cacatctttt gccccagcag caagggcttc ggcttgttca tcaagcggtt gttttggggc
    10441 gactaatcgc aaatgggtta agcccatcgt tttcattgct cgggctgcgg aaccgatatt
    10501 tgccgggagt gaagtttcaa caagaatgat ctgaatttgg ttgagaatct gcattggttt
    10561 tgtttctttt ttgttaaatt tagcctgtta atagtgtagc aaaaatttag cctgcttaaa
    10621 atttgaaaaa atgggaaata atcggtttaa ttgggcataa ttttttacct atttgtttta
    10681 tttagtggta catttagagc gtttttaatc acaacaacca tacaggagtc tttatgctta
    10741 ttggtgtacc aagagagctg ttagacggtg aaactcgagt ggcggcaacg ccaaaaaccg
    10801 ttgagcagat caaaaaactt ggctttgacg tacttgtgga acaagacgca ggttttaaag
    10861 ccagttttga agataataca tttttaaatg caggtgcaaa tatcggcaca caacaagaag
    10921 tttggaatgc agacattatt tttaaagtga atgcgccgac agaagctgaa attgagttaa
    10981 tcaaagaagg ggtaacctta gtcagcttta tttggccggc acaaaatcca caattgatgg
    11041 aaaaattgca gtctaagaaa attaatgtgt tggcaatgga cgctgtgcct cgtatttccc
    11101 gtgcccaagc tctcgatgct ttaagttcga tggcaaatat ttcgggttac cgtgcggtga
    11161 tcgaagcggc aaacgctttt ggtagcttct ttactgggca aattaccgca gcaggtaaag
    11221 taccaccggc aaaagtgttg gtaattggag ccggtgttgc tggcttggct gcaatcgggg
    11281 cggcaaatag cctgggggcg attgtgcgtg cctttgactc tcgtcctgaa gtaaaagagc
    11341 aagttaaatc aatgggggct gatttcctcg aaattgattt tgaagaagaa ggtggctcag
    11401 gtgacggcta tgcgaaagtg atgtcggaag agtttaaccg tcgtgcaatg gcgttatatg
    11461 ccgagcaagc caaagaggtg gatattatta ttaccaccgc tgccattccg ggcaaacctg
    11521 ctcctcgttt aatcactaaa gagatggttg attcaatgaa accgggttca gtgattgttg
    11581 atttagccgc tgcaaccggt ggtaactgtg aatacaccaa agccggcgag attgtggtga
    11641 ccgataatca agtgaaagtg attggctata ccgattttcc tgcacgttta ccaacgcaat
    11701 cttcgcaact ttatggtact aacttggtga atttactcaa actgttagca ccaaataaag
    11761 atggtcaaat tgagattaat tttgatgatg tagtgttgcg tggcgtaacg gtggtgcgtg
    11821 acggcgaatt aacttggccg gctccgccga ttcaagtatc tgcccaactg caaaaacaag
    11881 cagccgcacc acaagcggtc aaaaaagagg aaaaacctgc agaccctcga gtgaaatatg
    11941 gcgtgatggc agcagcaggt gtgctgttcc tatggcttgc atctgtggct ccggcagcgt
    12001 ttttatcgca ttttaccgta ttcgttctcg cttgtgtggt agggtattat gtggtttgga
    12061 acgtcagcca cgcactacat accccattaa tggcggtaac aaatgcgatt tcagggatta
    12121 tcatcgtagg tgcggtatta caaattgccc aaccgaccgg taatttcttt attgatattt
    12181 tggcatttat tgcgattttg gttgccagca tcaatatttt tggtggcttt aaagtaacgc
    12241 aacgtatgct tgcaatgttt agaaaaggtt aaggagaaac gaaactatgt cttttggatt
    12301 tgtgactgct gcgtacatta ttgcagctat tctctttatt atgagcttag ccggtctttc
    12361 taaacacgaa accgccaaag caggttgttg gtatgggatt gtgggtatgg cgattgcttt
    12421 agtggcaacc attttcggcc cgcaatcaca tggcacgatt tggatcttaa tcgcaatggc
    12481 aatcggtgga ttcatcgggg tgaaaaaagc cctaaaagta gaaatgaccg aaatgcctga
    12541 attagtggca attctacaca gctttgtcgg tttagcagcg gtattggttg gttttaacag
    12601 ctacggactt cataccaatg cattaatgcc tgcaaattta gatgaaactg cacaagcggc
    12661 atttttagcg gaacaagcca ctcttgccaa tatccacaat gtggaagtgt tcttaggtat
    12721 tttcattggt gcggtaacct tctccggttc attggttgcc tttggtaaac taagcggtaa
    12781 attatttggt cgtaaagtct cttctgcagc actgaatatt ccacataaac acaaatggaa
    12841 tttggcagcc attatcgttt ctgttttttt gatgatcgtg tttttaaata atccgcacaa
    12901 tatcttccct gtgctgatta tgacaattat tgcgttagtc ttcggctggc acttagtttc
    12961 ttctattggt ggggcggata tgccggtggt agtttcaatg ctgaattctt attcaggttg
    13021 ggcagccgca gcggcaggct ttatgctaag caacgattta ttaatcgtaa ccggtgcatt
    13081 agtgggatca tcaggtgcta tcctttctta tattatgtgt aaagcaatga accgctcatt
    13141 cattagcgtg attgccggtg gttttggcaa tgaagttcag tctggcaaag gtgatgaaga
    13201 atacggtgaa caccgtgaaa cgaaagccga agaagtggca gaaatgctga aaaatgcaag
    13261 ctctgtgatc atcacgccgg gttatggtat ggcagtggct caagcacaat atccggtagc
    13321 ggaaatcacc gcaaaattgc gtgaaaaagg cgtgaatgtg cgttttggta ttcaccctgt
    13381 ggcagggcgt ttaccggggc atatgaacgt attattggcg gaagcgaaag taccttatga
    13441 tgtagtgctg gagatggacg aaatcaatga cgattttgaa gaaaccgatg tggtattggt
    13501 aatcggtgca aacgataccg taaaccctgc tgcattagac gacccatcaa gccctattgc
    13561 tggaatgccg gtgcttgaag tatggaaagc acaaaatgtt atcgtgttca aacgttcaat
    13621 ggcagtaggc tacgccggtg tgcaaaaccc actgttcttt aaagaaaata cccaaatgct
    13681 atttggtgat gcaaaagaac gtgttgatga tattttacgg gcgttaaatt aaatttaaat
    13741 aaaaaagcga acataagttc gcttttttgt gcttaggcta acataaattg ttaatttttt
    13801 actctaatac catcaatccg ggaagagtcg caaaactctg cgattttacg gtatcatgga
    13861 aatcatctaa gtaggctagg gtagcatcgg cttctcgaat agcgccataa agggtgctat
    13921 atagtccttg ctgagttatt tttcttgcca ccacataccc cctatctaaa tatggtttaa
    13981 ccgcccaata aggtagtgca gcaatgcctc gccgacttgc tactagctga ataatagcaa
    14041 tagttaactc actattacgc cgagtaactt tgatgccagc agggcctaat atttttcgca
    14101 gtaaatctag catatcatca ggtactggat aggtaattaa tgtctcatct ttaaaatctt
    14161 cagcttccca tacatctttt gtggcaagag ggtgatcttt agagcaaata gctaccattt
    14221 catatgaaaa gagaggtttg tatataatac cagcactatc ttcctcttca gcgacaattg
    14281 ccagatctgc acgatggctt aatagcaaac caaccgtatc agtatggaaa cccgatacga
    14341 tatctaattc aactagaggc caatgttgac ggaaagtatc cattgccggc attagccaat
    14401 caaaacaggt atggcattct actgcaacac gtacctctcc gacttctcct tgtttcactc
    14461 gggctaaatc taattcagcc tcaatgactt tgggcaaaat atcgtaggcc aacttaatta
    14521 aacgttcgcc agctggtgta aagcgaatag gggagctttt tcgctcaaat agcgtaagat
    14581 catactgttc ttccagtaat tttatttggt gtgaaagtgc ggattgtgtt aaatggacac
    14641 gctttgctgc aagagatacg ctaccgctct cttttaatgc gattaatgtc cgcaaatggc
    14701 ggatttcaag aaaaatcggt ttcataattt attcatctaa aagtgaaaaa agatgaaaat
    14761 aattataaca aacaagcggt caaattctgc aaaaaatttg caaaatccaa ccgcttgtat
    14821 agagcagtgg cgaattattg ggttaattga actaaaatgc ggcgaacagg ctctgctgca
    14881 ccccataata attggtcacc tacggtaaat gcagctaagt attctccacc catcgctaat
    14941 ttacgcaagc gaccaaccgg cacgctcaat gtaccggtca ctttcgccgg ggtcaattca
    15001 cgcaatgtgg tttctttgtc gtttggaatt actttcaccc attcattgtg ggcagcaata
    15061 atttgctcga tttccgctaa cggaatatct tttttcagct taattgtgaa tgcttggctg
    15121 tggcagcgta atgctccaat acgcacgcat aaaccatcaa ctggaattgg gttatcgctt
    15181 aaacctaaaa ttttattagt ttctgcataa cctttccact cttctttagt ttgcccggtt
    15241 tccggtaata atttatcaat ccaaggaatt aagctaccgc ccaatgccgc accgaagttc
    15301 tcggttggga agtcttccgc acgcatttta gccgtaactt tacgttcaat atctaaaata
    15361 gaagaggcag gatttgctaa ttcagctttt acgctatctt ctaattcacc catttgagaa
    15421 agcaattcac gcatattttt cgcaccggca ccggaagcgg cttggtaagt tgctacagaa
    15481 acccactcga ccaaatcttt ctcaaataaa ccaccgatag ccattagcat taggcttacg
    15541 gtacagtttc cgcctacaaa tgttttaatg ccgtttttta agccttctga aatcacatgt
    15601 tggttcaccg ggtctaatac gataatagca tcgtctttca tacgtaatgc agaagccgcg
    15661 tcaatccaat agccgttcca accggtttct tttaattttg gatagacttc atttgtataa
    15721 tcaccacctt ggcaagttac gataatgtct aattttttta attcttcaat gtcaaaggcg
    15781 ttttttaatt cgccggcatc ttttccgcca aaaacagggg ctttttgacc cgcttgtgaa
    15841 gtagtgaaaa aaacagggtt gatattggcg aagttatttt cttcaaccat acgatccatt
    15901 aagaccgaac cgaccatacc acgccaaccg ataaaaccta cattttgc
//
LOCUS       DANLYI010000040        15667 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.040, whole genome shotgun sequence.
ACCESSION   DANLYI010000040 DANLYI010000000
VERSION     DANLYI010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 15667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15667
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.040"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            <1..2941
                     /gene="ptrA"
                     /locus_tag="PXD81_000909"
     CDS             <1..2941
                     /gene="ptrA"
                     /locus_tag="PXD81_000909"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HDL5536956.1"
                     /translation="LSFLTQFVAAAFALNVMTYPAFAEVAKNSEKTTASQPQSQLGFE
                     LIKQSINKSPNDKAIYQGIKLDNGMEVLLISDSQANKSLMSAIIPIGSMDDPISQQGL
                     AHYLEHMILMGSKNYPETNSLDGFLTKNGGMNNASTASHRTAYYLQVNNEAFSEAVTR
                     LADTLAFPLLLETNAKKEVNAVNAEMVRAKSHDGHLLHSVNLATANPAHPATKFAVGN
                     KETLSDKPDSKLQTELEQFYKQHYSANLFKVVLYSNQSIEQMAKLAANTLGKMENKHF
                     SIPQTAEPRYRDEDKSVFIQYKPVKSNKMLVIGFDFPNDEMQFKHKTGKYIAYMLNNN
                     TDGTLSDYLIKQGLSDGGIEAVSSANIGRDRGSFDIYIELTDKGLSEQDQIISLVFQQ
                     IEKIKQEGIQESYFSEVKESLKQDFQHLQVEKNMDFVEFIADQMLHFPLEHVIDQPYL
                     TETMDNEAIKAKLAAMNIDNARIMLVSDTAKTDKKTPYFEAGYSVAKITQEQKQKWLD
                     FSKNPQLNLPELNPYFTTDFTLNKVETGLTKPKLINDQAGNLIYTMPSVYFTNEPKAK
                     IGVGFNIRPRKDDLKSLTAASILGYMNNLAQTKLDFQSSVAGMNLSLAPAANGLALSA
                     EGYTQNLAKLMLDSVKQFSRFEFNEDVLAQAKQRLHQELDRNEKENSLNQANAALSRF
                     ASYPYFDVPKQREMIDSITLADIEQLRQRILQQSSGFKLLSVGNLSAEQVSALAEDLQ
                     AVIKNQKSANATGQYVDVSTSQRKINVIKSVPNEDNALLMAFMPNGYNELESLTRAKL
                     LRSIISRWYFDDLRTDKQLGYVVYATDNIIGKTAGIQFMVQSPNTTPAGILEHNERFF
                     TQSFERLKNLSDEEFGKYRDSLVEKLQYKPESLAEEFSEFGSDFSRNNPKFDRLQQVI
                     ELTKALTKADIVAFYQKAVIEKQGFVFVSQAVGTKTKAEDVAKFDGFEQIESIEKLQR
                     EFEIKDY"
     gene            complement(2990..4636)
                     /locus_tag="PXD81_000910"
     CDS             complement(2990..4636)
                     /locus_tag="PXD81_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005606119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL5536957.1"
                     /translation="MKFPTLTKISASVLAVLALAACDDKSNENKPTAAAAKPQTEKTF
                     VNCVSRSPTGFSPILMMDGLSYNASSQQIYNRLVEFVPGTTDIEPALAESWEISEDGL
                     TYTFKLRKGVKFHSNKDFTPSRELNADDVVFSFNRQLDKNHPYHTVSNGTYPYFNAMK
                     FPTLLKSVEKVDDSTVRITLTKRDASFLSSLGMDFTSIYSAEYADKMMKAGIPEAVDT
                     TPIGTGPFVFSGYVLDQASRYVANKEYWKGKPDIDRLIFEIVPDATTRYAKLQSGQCD
                     LMDFPNATDIEKMKNDPKINLLSQPGLNIAYVAFNTEKAPFDNVKVRQALNLAVDKKA
                     IIDVVYQGNGFAAKNPLPPTIWGYNDSLADSEYNVEKAKQLLAEAGFPNGFETELWVQ
                     PVVRASNPNPRRMSEIIQADWEKIGVKAKLVTYEWGDYIKRTKAGELTAGTYGWSGDN
                     GDPDNFLSPLFGTPNIGNSNYARFTNPELDALLEKALGSSDKAERTKLYEQAQVILRE
                     QAPWINVAHSINFAPTSKRVEGYKQSPFGYTYLYPVKIVD"
     gene            4712..6802
                     /locus_tag="PXD81_000911"
     CDS             4712..6802
                     /locus_tag="PXD81_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent
                     hemoglobin/transferrin/lactoferrin family receptor"
                     /protein_id="HDL5536958.1"
                     /translation="MIFANQSIELDTVNVIATRDPSKFADTPQKQTKDALLVKQATSV
                     AAALKALPNVDVQGGARAIVQKPSIRGLSDNRIVQVIDGVRQNFDLAHRGSYFVPMSL
                     IQEIEVIKGPSSSLWGSGALGGVVAMRTPNALDLLKNNDKFGAKIRQGYQTANNLSET
                     EASVFAANDRFDALLSGFYNNADNLRSGEGKELNNTGYKQAGGLVKFGWQINDTHRLE
                     LSHRHSQFKQTAPGNNEVENQFTDNDVSAQISAWHAANPGNPSNPVYLAKMLAFYGQL
                     HSSLGSVSYLSDQKITDKSTSLNYYFNPSNPYLSTQVTLYSNSTTENEHRKISGVEDQ
                     TKLSTRGVNIRNHSELSWFSLVYGVDYMLDKVATQRGRNNTDAQYRANPYDAKSKTTG
                     AYLIAHIPLFDEKVVFSPSVRYDRFDTSSETVKYKDSHWSPAAKLTWKATNWLDLTAK
                     YNEAFRAPSMQERFVSGSHFGTTVRGLPINNIFLTNPNLRPETAKNKEVTFNVHFDNV
                     ISQGDKFNFEATYFRNDVKDLINLQVFKANPTSPMEFIPTRSQYQNIANARLSGIELQ
                     TRYQTERLAVFANYGSTKGKDKDSGEALSNIAASKIGFGVNYAVVQDKFTVGANVTRY
                     QAQHRVPKNHGVTYQGYTLTDLHATYAPLKGEWKNLRLDVAVENLFDKKYQPAFSLME
                     GSGRNVKLSAAYSF"
     gene            6873..8561
                     /locus_tag="PXD81_000912"
     CDS             6873..8561
                     /locus_tag="PXD81_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005694040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
                     /protein_id="HDL5536959.1"
                     /translation="MKFYPSLIASAVSFAVVLSGQAAVNVPDAGSLQRELDQRQAQQK
                     VKPSGEWFNQAAKAPYTATDGQELTFPLTQVQVFDRTNQEISTPELAPILANYVGKAV
                     SLTDLSNLANEITEFYRSHNYLVAKAILPPQEIEQGKVKILLFKGNVGEVRLQNQSAL
                     SDKFVARLANTTVNGSEFILKDELEKFALTMNDVPGVNTGLELSAGKQAGEANLLIKL
                     QNAERFSGYVSADNQGNKNTGRYRLATGIKANNLIGWGDELKVDLMSSNNANLKNARL
                     DYSSLIDGYATRLGLTANYLDYELGGNFKALQSQGHTHNLGAYVLHPTIRLPDFRLNT
                     KVSFNHQRLTDKQQAVNVAQKRQVNSLTVGINGSWNSLKDGTSYFSLSALFGNLANHT
                     NEQAHYVATDFQPKSRFTVYQYSLSHEQALPKSFAFNLGVNGQFADKTLDSSQKMLLG
                     GLYGVRGHQAGVASVDEGHLVQTELKHYLPLFSKSLLTSSVFYDYGWGKYYKHSGFLA
                     RGVNNSVKLQSIGAGLSLSEAGSYAINASVAKPLGKKIENADKHQFWFSAIKTF"
     gene            8573..11389
                     /locus_tag="PXD81_000913"
     CDS             8573..11389
                     /locus_tag="PXD81_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005694039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="filamentous hemagglutinin N-terminal
                     domain-containing protein"
                     /protein_id="HDL5536960.1"
                     /translation="MFKRNAISLCILTACSGLAYANTADLPQGERVRVGTATIERTTD
                     KMTVNQSSNKVQIDWNSFDIGKNKEVEFKQPTEMAVAYNRVTGGNASQIQGKLTANGK
                     VYLANPNGVLITKDAEVNVGGLLVTTKDLEKISENGTKDKFSRKLAKEGKILNQGKIT
                     AKGERAFVVLVGDDVANEGEINAKNYYHKETKKVTECYDDSWSLVKQCYDFDKEIVKS
                     TSGEVHLSSGENVTFTLSDRYIDVALDDNTVNSIIKNDGAIIANGDITLTAKGRNQAL
                     DSLIINNGVLQANKVQNKNGTIILSAGETQLHEKSQIKGEKVSFAEASYYGPTEDIKV
                     VSKTGSKVTAPVIDFKGKTVNIQGEFGREDSTDLYNDELQILKTTVNIEVPNTEDIRI
                     ADTDNGGSGSFIQAGALASLLANNGRVNVQGKGFDVSGKLNVNAFKQTDSVLKFINQA
                     GINIHNADIHANGRLFFVTNLPNDTDFQSNIRITDSKINLGNGSMGFGRSSKRWEMHD
                     VVRRADTELRKKFNLDMKNVELNQVDDFVVAGGFQQVNLDNVKARGKTNFYLDSGNSR
                     MFVGEQIYEYGVTDIEKRTQLANLDQRRKRWNSDNYDETDLNWEYNQRFDGFAAKHNH
                     RAPIADTEINIANSDISLDNGFVHLMAEKINLDDSKINITFNQDNSLDESTQINRLGL
                     NGKVSMKNSHINVVGDEKDGISPARHYATMFLVGELLGEKSSIFLKSHQGAVLRTDSN
                     VTIAGKNSKDDLKITAITTGKKMGNEIIGPDGLPTSEDNDANASNTAFAIGYEPNATA
                     IVENASITALAPNGGLAYTGTSKTQIDAKDNATFTFFEKPRAGYDKLLGDIKGNPDKL
                     TPRDFARLYDNIKGIKASSLSEQQLGLADTAKKTSEHTLANSLDVENLVSVTVCDEQN
                     KCEERMLGNKNTNAKVIVGDIKFE"
     gene            11512..11643
                     /locus_tag="PXD81_000914"
                     /pseudo
     CDS             11512..11643
                     /locus_tag="PXD81_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015587011.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(11698..12459)
                     /locus_tag="PXD81_000915"
     CDS             complement(11698..12459)
                     /locus_tag="PXD81_000915"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HDL5536961.1"
                     /translation="MKLNQAQIQNALICGQAVVFDEIDSTNEYLLTHHQRLENGTVCL
                     AEKQTAGRGRRGRTWYSPESENLYFSILWHYPMEQAADLPPLSLVVALIIAESLTVQN
                     VKDIQIKWPNDIYYQGKKMGGILLETQANRNGLDVVIGIGLNLGMAKVDENIVTQAWA
                     DLSQYHFNRNELVCRLAYELQKNLKIYPLVGFAHYAERWQSFDLFRHKAVKLITEAEE
                     IHGISQGINEQGELILKQGDNLRYFGIGEISLRAD"
     gene            complement(12471..13502)
                     /gene="murB"
                     /locus_tag="PXD81_000916"
     CDS             complement(12471..13502)
                     /gene="murB"
                     /locus_tag="PXD81_000916"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HDL5536962.1"
                     /translation="MMKHSLTPFHTFHLPAKATQIIEFTTVEQLLSEWQKAFNAQLPI
                     LILGQGSNVLFLEDFDGVVLVNKLKGITHHEDEHFHYLHVQGGENWHELVKWTLAHNI
                     AGIENLALIPGVASSAPIQNIGAYGVEFEQVCDFVEVLNLRSGELFKLSKSECEFGYR
                     ESVFKHQYRDEFAIISVGLKLAKAWKPVLSYGSLAQLNPETVTPIQIFDEVCAVRSSK
                     LPNPDEFGNAGSFFKNPVISNEQFAPIQAKFPAIPHYPQADGTVKLAAGWLIDQTGLK
                     DLQIGGAAVHTQQALVLINKENATGQDVLALAKTVRQRVKEKFGVEIQPEVRFIGKNG
                     EVDSEKITR"
     gene            complement(13606..14280)
                     /gene="crp"
                     /locus_tag="PXD81_000917"
     CDS             complement(13606..14280)
                     /gene="crp"
                     /locus_tag="PXD81_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010787261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HDL5536963.1"
                     /translation="MQDMTLSTAPLENVTPPPSIHDPAVEWFLTHCHIHRYPAKYTLI
                     HAGEDAESLFYIVNGSVSVYIKDDETKEMLLTNLGTGEFVGELSLFEEKVQQRTAYVR
                     TKGPCEIAEISYKKFKQLVHLNPDILMYLSAQMARRLRQTSRQVGNLAFLDVAGRIAQ
                     TLLNLAKMPDAMTHPEGMQIKITRQEIGQMVGCSRETVGRIIKMLEDEGLISAHGKTI
                     VVYGAR"
     gene            complement(14342..14560)
                     /locus_tag="PXD81_000918"
     CDS             complement(14342..14560)
                     /locus_tag="PXD81_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018650836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HDL5536964.1"
                     /translation="MIIPWQELEESTLHNILDSFILREGTDYGERELSLAEKRERLLT
                     QLKADKVVIVWSELHESLDIKDKKSFLG"
     gene            complement(14563..15168)
                     /gene="slmA"
                     /locus_tag="PXD81_000919"
     CDS             complement(14563..15168)
                     /gene="slmA"
                     /locus_tag="PXD81_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005624468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HDL5536965.1"
                     /translation="MTEPKIKMPKKSVVERRQQVLEVLISLLNSEEGMQRITTERLAA
                     AVGVSEGALYRYFPSKTKMFEALIEKIEQTLTSYIHANKRQDSSANSVRAILYVILEF
                     ARKNPGVTRILTGHALMFEEELLKARVAKFFDNLELQFANILQLSKLRERKSFEDERA
                     LAGYLVNFCEGQFLRLVRSNFSYNQHQHFEKQWAFIKPLFD"
     gene            complement(15180..15635)
                     /gene="dut"
                     /locus_tag="PXD81_000920"
     CDS             complement(15180..15635)
                     /gene="dut"
                     /locus_tag="PXD81_000920"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014550997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HDL5536966.1"
                     /translation="MKQIDLKILDSRIGNEFPLPAYATEGSAGLDLRAMLNEPLTVKA
                     GETVLIPTGISIYIADPNLAAVILPRSGLGHKNGIVLGNLVGLIDSDYQGELKVSLWN
                     RSQADFTVNVGDRIAQLVFVPVVQASFNIVQEFEQTDRGEGGFGHSGKQ"
BASE COUNT         4678 a         3311 c         3430 g         4248 t
ORIGIN      
        1 attatctttt cttacccaat tcgttgcagc ggcttttgcc ctcaatgtga tgacttaccc
       61 tgcatttgcg gaagttgcaa aaaattctga aaaaacgacc gctagtcaac cccaatccca
      121 actcggtttt gagctgatta aacagagcat taacaaaagc ccgaatgata aagcgattta
      181 tcaaggtatt aaacttgata atggaatgga agtgctactg atttccgaca gtcaagcgaa
      241 taaatcgttg atgtctgcca ttattccgat tggctcaatg gatgacccga tttctcagca
      301 aggtttagcc cactatcttg agcatatgat cttgatgggc tcgaaaaact atccggaaac
      361 caacagctta gacggttttt taaccaaaaa tggcggtatg aataatgcct cgactgcttc
      421 tcaccgcacg gcgtattatc tacaagtcaa taacgaagcc tttagcgaag cggtaacacg
      481 actggcggat acgttagctt tcccgttatt gctggaaacc aacgccaaga aagaggttaa
      541 tgcggtcaat gcggagatgg tgcgagctaa atcgcacgat gggcatttat tgcacagtgt
      601 taatttagcc accgcaaacc cggctcatcc tgctaccaaa tttgcggtag gcaataaaga
      661 gacgttatct gacaagccgg atagcaaatt acaaaccgag ttggagcaat tttataaaca
      721 gcattattct gcgaatctgt ttaaagtggt gctttattcc aatcaatcga ttgagcagat
      781 ggcaaaattg gcagccaaca ctttaggcaa aatggaaaac aaacatttca gcataccgca
      841 aaccgctgag ccacgttatc gtgatgagga caaatcggta tttattcaat ataagccggt
      901 gaaatccaac aaaatgttag tcattggctt tgattttccg aatgacgaaa tgcagtttaa
      961 gcacaaaacc ggtaaatata ttgcttatat gttaaacaat aatactgacg gcacgctttc
     1021 ggattacttg attaaacagg ggttgtctga cggcggtatt gaggcggtta gcagtgccaa
     1081 tatcgggcgg gatcgaggct cgtttgacat ctatattgaa ctgacagaca aaggtttgag
     1141 cgagcaagat caaattattt cattggtttt ccaacaaatt gagaagatca aacaagaagg
     1201 cattcaagag agctatttta gcgaagtgaa agagagcctg aagcaggatt tccaacattt
     1261 gcaagtggaa aaaaatatgg atttcgtgga atttattgcc gatcaaatgc tccatttccc
     1321 gttagaacac gttatcgatc agccttatct gaccgaaaca atggataacg aggcaattaa
     1381 agcaaaattg gcggcaatga atattgataa tgcccgcatt atgttagtca gcgatactgc
     1441 caaaaccgac aagaaaaccc cttattttga agcaggttat tcggtggcaa aaatcacgca
     1501 agaacaaaag caaaaatggc tggatttcag taaaaatcca cagctcaatt tgcctgaatt
     1561 gaacccgtat ttcactaccg attttacctt aaacaaggtc gaaaccggct taactaaacc
     1621 gaaattgatt aacgaccaag cggggaattt aatctataca atgccgagcg tgtattttac
     1681 caatgaaccg aaggctaaaa tcggcgttgg ctttaatatt cgcccgagaa aagatgactt
     1741 aaagagcctg accgcagcgt ctattttggg ttatatgaac aatttagccc aaaccaagtt
     1801 agattttcag tcttcggtgg cgggtatgaa tttaagttta gctccggcgg caaacggttt
     1861 agccttaagt gcagagggtt atacccaaaa tctggctaaa ttaatgctag acagcgtaaa
     1921 acagttcagc cgctttgagt ttaacgaaga cgtgttggca caggctaaac aacgtttgca
     1981 ccaagagctg gatcgtaacg aaaaagaaaa cagccttaat caagctaatg cagcattaag
     2041 ccgttttgcc tcttaccctt attttgatgt gccgaaacag cgtgagatga ttgatagcat
     2101 tacccttgcg gatattgagc aactacgcca gcgaattttg cagcagagca gcggctttaa
     2161 gttgttatcg gtggggaatt tgtcggctga gcaggtttct gctctggcag aggatttaca
     2221 agcggtcata aaaaaccaaa aatctgcaaa tgctaccgga cagtatgtgg atgtgagtac
     2281 cagtcagcgt aagattaatg tgattaaaag cgtgccgaat gaagataatg cgttgctaat
     2341 ggcatttatg ccgaacggct ataacgagct ggaaagcctt actcgagcca aattgctcag
     2401 aagcattatt tcccgttggt attttgatga tttgcgtact gataagcaac taggctatgt
     2461 ggtttatgcg acagataata tcatcgggaa aaccgccggt atccagttta tggtacaaag
     2521 cccgaatacc acaccggcag ggattttaga gcataacgaa cgtttcttta cacaaagttt
     2581 cgaacgttta aaaaatctat ctgatgagga atttggaaaa tatcgtgata gcctagtgga
     2641 aaaactgcaa tataaaccgg agtctttagc ggaagagttt tctgagttcg gttcagactt
     2701 tagcagaaat aacccgaaat ttgaccgctt gcaacaggtg atcgaattaa ccaaagcact
     2761 caccaaagca gatattgtgg cattttacca aaaagctgtg attgaaaagc aaggcttcgt
     2821 gtttgtcagc caagctgtcg gcaccaaaac caaagctgaa gacgttgcga aatttgacgg
     2881 ttttgaacag atcgaaagca ttgaaaagct acaacgagag tttgagatta aggattatta
     2941 gagagttatt agagagagtt aggggcgaaa aattttcgcc cctcattttt taatccacaa
     3001 ttttcaccgg atataagtaa gtatatccga acggactttg tttgtagcct tcaacacgtt
     3061 tgctggttgg ggcaaagtta attgagtgag ccacgttaat ccacggagct tgctctctta
     3121 agataacctg tgcctgctcg tataacttag tacgttctgc tttatcagat gagcctaatg
     3181 ctttttctaa taacgcatct aactcagggt tagtaaagcg agcatagtta ctgttgccga
     3241 tatttggcgt gccgaatagt ggcgataaga agttatccgg atcgccgtta tcgcccgacc
     3301 aaccgtaagt tccggcggtt aattcaccgg ctttggtacg cttgatgtaa tcaccccatt
     3361 cataggtgac taattttgct tttacgccga ttttttccca gtcggcttga ataatttcag
     3421 acatacggcg tgggttcggg ttagaagcac gcaccacagg ctgcacccat aattcggttt
     3481 caaaaccgtt cgggaagcct gcttcagcta atagttgctt cgctttttcc acgttatatt
     3541 ctgaatccgc taagctgtcg ttatagcccc aaatggttgg cggaagtggg tttttcgccg
     3601 caaaaccgtt accttggtaa actacatcaa taatcgcttt tttatccact gctaagttta
     3661 acgcttggcg aactttcacg ttatcaaacg gagctttttc ggtattgaat gccacataag
     3721 caatgttcaa acccggttga gaaagtaaat tgattttcgg atcatttttc attttctcaa
     3781 tgtcggttgc atttgggaag tccattaaat cacattgacc ggattgtaat ttcgcataac
     3841 gggttgttgc atccggcacg atctcaaaaa tcaaacggtc aatgtcaggc ttacctttcc
     3901 aatactcttt gtttgctaca taacgactgg cttggtctag cacataaccg ctaaacacaa
     3961 acggacctgt gccgattggc gtggtatcta ccgcttcagg tatgccggct ttcatcattt
     4021 tatccgcata ttccgccgaa taaatggagg tgaaatccat accaaggctg gataagaatg
     4081 acgcatcacg tttggttaag gtaatacgaa cggtgctgtc gtccactttt tccaccgatt
     4141 tcaataatgt cgggaatttc attgcgttga aatacggata agtgccgtta gagacggtgt
     4201 gatacggatg atttttatct aactgacggt taaatgaaaa tactacatcg tccgcattca
     4261 attcacggct tggagtgaag tctttgtttg aatggaattt cacgcctttg cgtaatttaa
     4321 aggtgtaagt taagccgtct tcgctgattt cccagctttc tgccaatgcc ggttcaatat
     4381 cggttgtacc cggcacaaat tccaccaagc ggttgtaaat ttgttgcgag ctggcgttat
     4441 aagataaacc gtccatcatt aaaatcgggc taaaaccggt tggcgaacgg ctaacgcagt
     4501 ttacaaaggt tttctcggtt tgcggttttg ctgctgccgc agtaggttta ttttcgttgc
     4561 ttttatcatc gcaagccgct agagctaata ccgctaaaac gcttgcacta attttggtta
     4621 acgttggaaa tttcatatta ttctccgctt aatcttagga ttaacttacg aagattacct
     4681 tttccacaat gaggagtaaa gaacaaaaag cattattttt gcaaatcaat ccatagaatt
     4741 agataccgtc aacgtcattg ccacccgtga tccaagtaaa tttgccgata cgccacaaaa
     4801 acaaactaaa gatgcacttt tggtaaaaca agcgacgagt gtggcggcgg cattaaaagc
     4861 gttgccgaat gtggatgtgc aaggaggggc ccgtgcgatt gtgcagaagc cgtctattcg
     4921 tgggttgagc gataatcgta ttgtgcaggt gattgacgga gtgcgtcaga attttgattt
     4981 ggctcaccgt ggttcttatt ttgtgccgat gtcattgatt caagaaattg aagtaatcaa
     5041 aggtccaagc agctcgttgt gggggagcgg tgccttaggt ggggtggtgg caatgcgcac
     5101 accgaatgcg ttggatttat tgaaaaataa tgacaaattc ggggcaaaaa tccgtcaagg
     5161 ttatcaaacg gcgaataatc tttccgaaac cgaagcctcg gtgtttgcag caaatgatcg
     5221 gtttgatgcc ctgctgagtg gtttctacaa taatgctgac aatttgcgta gcggtgaagg
     5281 caaggaactg aataacacgg gctataagca agccggcggt ttagtaaaat tcggctggca
     5341 aattaacgat actcatcgtt tggaattatc ccatcgtcat agccaattta aacaaactgc
     5401 accgggtaat aacgaggtag aaaatcaatt taccgataat gatgttagtg cacaaattag
     5461 tgcttggcat gcggctaatc ctggaaatcc aagtaatcca gtatatcttg cgaaaatgtt
     5521 agccttttac ggtcaattac attcaagtct tggtagtgtg agttatttaa gtgatcaaaa
     5581 aattaccgat aaaagcacct cacttaacta ctatttcaac ccaagcaatc cttatttgag
     5641 cacacaagtg acattatata gcaatagtac cactgaaaac gaacatcgaa aaataagtgg
     5701 tgtggaagat caaactaaac taagcactcg tggtgtgaat atccgtaatc attccgaatt
     5761 gtcttggttc tccttggtgt atggcgtgga ttatatgttg gataaagtag ccactcagcg
     5821 tgggagaaat aatacggacg cacaataccg tgctaatcct tatgatgcta aatctaaaac
     5881 tacaggtgct tatttaattg cacatattcc gctgtttgat gaaaaagtgg tcttttctcc
     5941 gagcgtgcgt tacgaccgtt tcgatacttc aagtgaaacg gtgaaatata aagacagcca
     6001 ttggtctcct gctgcaaaac ttacttggaa agcgacaaat tggttggatt taaccgcgaa
     6061 atataacgaa gctttcagag ccccttctat gcaagaacga tttgtgagcg ggtctcattt
     6121 tggtaccaca gtgagaggat tacctattaa taatattttc ttaactaacc ctaatttacg
     6181 cccggaaacg gcaaaaaata aggaagtaac cttcaatgta cattttgata atgtcatctc
     6241 gcaaggagat aaatttaact ttgaagcgac ttatttccgt aatgatgtga aggatttgat
     6301 taatcttcaa gtattcaaag caaatcctac aagcccaatg gaatttattc ccactcgttc
     6361 ccaatatcaa aatattgcga atgcacgttt gagtggtatt gaattacaaa ctcgttatca
     6421 aactgaacgt ttggcagttt ttgctaacta cggtagtaca aaaggtaaag ataaagacag
     6481 cggggaagcc ttgtcgaaca ttgctgcaag caaaatcggc ttcggggtca attatgccgt
     6541 ggtgcaagac aaattcacgg tgggggcaaa cgtaacccgt tatcaagcac aacatcgtgt
     6601 gccgaaaaac cacggcgtga cttaccaagg ctatacgctg accgatctac acgccactta
     6661 cgccccatta aaaggcgaat ggaaaaacct gcgtttagac gtggcggttg aaaacctatt
     6721 cgataaaaaa taccaaccgg cattcagctt aatggaaggc agcggtcgta atgtgaaact
     6781 cagtgcggct tacagcttct aatgtcttta acggaagacg gcacaagcgg tcgttttccg
     6841 gttatttttt gcaatattaa aagggagtta ctatgaaatt ttatccgtcg ctgatcgcaa
     6901 gtgcggtcag ttttgccgtt gttttgagtg gacaagcggc agtgaatgtg ccggatgccg
     6961 gctcgttaca gcgtgaatta gaccaacgtc aagctcaaca aaaagtcaaa ccgagtggcg
     7021 aatggtttaa tcaagcggca aaagcacctt ataccgcaac ggacggtcaa gaactgacat
     7081 ttccattaac tcaggtacaa gtttttgatc gaaccaacca agaaatctct accccggaat
     7141 tagccccgat tttggcaaac tatgtgggta aagcggtttc gctgaccgat ttaagcaact
     7201 tggcaaatga gatcaccgag ttttatcgta gccacaatta tctggtggca aaagccattt
     7261 taccgccaca ggaaattgaa caaggtaagg tgaaaatcct gttgtttaaa ggcaatgtcg
     7321 gtgaagtgcg gttacaaaat cagagtgctt taagcgataa atttgttgca cgccttgcca
     7381 acaccacagt gaatggttcg gaatttatcc taaaagacga attggaaaaa tttgccctca
     7441 cgatgaatga tgttcccggg gtgaataccg gcttggagct ttctgccggt aagcaagcag
     7501 gcgaggcgaa tttgctgatt aagctccaaa atgccgagcg tttctcaggc tatgtatcgg
     7561 cagataacca aggcaataaa aataccggac gttaccgttt agccacaggg ataaaagcca
     7621 ataatctgat aggttggggc gatgagttga aagtggattt gatgagttcc aacaacgcta
     7681 atttaaaaaa tgcgcgtttg gattattcga gcttgattga cggctacgct acccgtttgg
     7741 gcttaacggc gaattatctc gactacgaat taggcggtaa ctttaaagcc ctgcaatccc
     7801 aagggcatac tcacaatctt ggggcttatg tgttgcaccc gacaatccgc ttgccggatt
     7861 ttcggttaaa caccaaagtc agctttaatc atcaacgttt aaccgacaaa caacaagcgg
     7921 tcaatgtggc acaaaaacgc caagtaaaca gcttaacggt gggcattaac gggtcgtgga
     7981 attcgcttaa agacggcaca agctatttct ctctgtctgc cttgttcggc aatttagcta
     8041 accacaccaa cgagcaagcg cattatgtgg caacagattt tcagcccaaa tcccgtttta
     8101 cggtctatca atacagcctg tctcacgaac aagcgctgcc aaaatccttt gccttcaacc
     8161 ttggcgtgaa cgggcagttt gcggataaaa ccctcgacag ctcacaaaaa atgttactgg
     8221 gcggtttata cggcgtgcgt ggtcatcaag ccggtgtcgc ttcggtggat gaggggcatt
     8281 tagtgcaaac cgaactcaag cattatttac cgctcttttc caaaagcctg ctgaccagct
     8341 cggtcttcta tgattatggt tggggaaaat actataaaca cagcggattt ttggcgcgtg
     8401 gtgtaaacaa tagcgtgaaa ttacagagta tcggagcagg gctttcgctc tctgaagcag
     8461 gcagttatgc cattaacgcc agtgttgcca aaccgctggg taaaaaaatc gagaatgcag
     8521 ataaacacca attctggttc tccgccatca aaaccttcta aggagacaaa ctatgtttaa
     8581 acgtaatgca atttctctct gcattttgac cgcttgcagc ggtttagcct atgccaatac
     8641 agcagatttg ccgcaaggtg aaagggtgcg tgtcgggacg gcaacgattg agcgcacaac
     8701 cgataaaatg accgtgaatc aatccagcaa taaagtacaa attgattgga acagctttga
     8761 tattggtaag aataaagaag tggaattcaa acagccgaca gagatggcgg tggcatataa
     8821 ccgtgttacc ggcggaaacg ccagtcaaat tcaaggcaag ctgaccgcaa acggcaaagt
     8881 gtatttagcc aacccgaacg gtgtactcat caccaaagat gcggaagtga atgtgggcgg
     8941 tttgttagtg accacaaaag atttagaaaa aatcagcgaa aacggcacaa aagataaatt
     9001 cagtcgcaaa ttagcgaaag aaggcaaaat tcttaaccaa ggtaaaatta cggccaaagg
     9061 cgagcgggct tttgtggtat tggtgggcga cgatgtggct aatgaagggg aaattaacgc
     9121 caaaaattat taccacaaag aaacgaaaaa agtcaccgaa tgttatgacg attcttggag
     9181 cttggtaaaa caatgctacg attttgataa agaaatcgtg aaaagtacca gtggagaagt
     9241 gcatttatcc tccggagaaa atgtcacctt tactttaagc gaccgctata ttgatgtggc
     9301 gttagacgat aatacggtca atagcattat caaaaatgac ggggcaatca tcgccaatgg
     9361 tgacattacc ctcactgcca aagggcgaaa tcaagcctta gacagcttaa ttatcaataa
     9421 cggcgtattg caagccaata aggtgcaaaa taaaaacggt acgatcattt tatctgccgg
     9481 tgaaacccaa ttacacgaaa aaagccaaat taaaggcgag aaagtgagct ttgccgaagc
     9541 ttcttattac ggtccgacag aagacattaa agtggtttcc aaaaccggct caaaagtgac
     9601 cgcacctgtg atcgatttca aaggtaaaac cgtgaatatt caaggggaat ttgggcgtga
     9661 agacagcacc gatctctata acgatgaact acaaatcctc aaaaccacgg tgaatattga
     9721 agtcccgaat acggaagata ttcgcattgc agataccgac aacggaggta gcggttcctt
     9781 tatacaagcg ggtgctttag cctcattact tgcaaataac ggacgggtta atgtacaggg
     9841 caaaggtttt gatgtctcag gcaagttgaa tgtgaatgcc tttaaacaaa cggattccgt
     9901 gctgaaattt atcaatcagg cgggcatcaa tatccacaat gccgatattc acgcaaacgg
     9961 gcgtttattc tttgtgacaa acttgccgaa tgacaccgat ttccaatcga atatccgcat
    10021 taccgacagc aaaatcaact taggcaacgg ctcaatgggc tttggtcgca gtagcaaacg
    10081 ttgggaaatg cacgatgtgg ttcgacgtgc cgatacggaa ctgcgtaaaa aattcaatct
    10141 ggatatgaaa aacgtggaat taaaccaagt ggatgatttt gtggtagcgg gcggtttcca
    10201 acaagttaat ttggataacg tgaaagcccg tggcaaaacc aacttctatc tggattccgg
    10261 caactcccgc atgtttgtcg gtgagcagat ttatgaatat ggcgtaaccg atattgaaaa
    10321 acgcacccaa ttggcgaatt tagaccagcg tagaaaacgt tggaattcgg ataactatga
    10381 tgagaccgat ctcaactggg aatacaacca acgctttgac ggctttgcag caaaacacaa
    10441 tcaccgcgcg ccgattgccg atacggaaat caatattgcc aatagtgata tcagcttaga
    10501 taacggtttt gtgcatttaa tggcagaaaa aatcaatctg gacgacagca aaatcaatat
    10561 caccttcaac caagacaaca gcttggatga aagtacccaa atcaaccgct taggactaaa
    10621 cggcaaagtg tcgatgaaaa acagccatat taacgtagtg ggcgatgaaa aagacggtat
    10681 ctcaccggct cgccattatg ccacgatgtt cttagtgggg gagctgcttg gtgaaaagag
    10741 ttctattttc ctcaaatccc accaaggtgc agtattgaga accgacagca atgttacaat
    10801 tgccggtaaa aacagcaaag acgacctcaa aattaccgcc attaccaccg gtaaaaaaat
    10861 gggcaatgaa atcatcggac cggacggctt gccaacctcg gaagataacg atgccaacgc
    10921 cagcaacacc gcctttgcca tcggctatga acctaacgct acggctatcg tcgaaaatgc
    10981 aagcattacc gcattagcac caaacggcgg cttggcatac accggcacgt ctaaaaccca
    11041 aattgacgcg aaggataatg ctacctttac cttctttgaa aaacctcgag ccggctatga
    11101 caaattgctt ggcgacatca aaggtaaccc cgataaatta accccacgcg atttcgcccg
    11161 tttatacgac aacattaaag gcatcaaagc cagcagccta tccgaacaac agcttggcct
    11221 agcagacacc gcgaagaaaa ccagcgaaca caccttagca aacagcctag acgtagaaaa
    11281 tttggtttca gtgacagtgt gtgatgagca gaataagtgt gaggagcgta tgttaggtaa
    11341 caaaaatacc aatgctaaag ttattgtcgg agatatcaaa tttgagtagc gttgaagaaa
    11401 gtggtagaag ctcagacttg atctgataaa gcactataaa aagtgagagt ttcccgttta
    11461 gaatatgagt gtcaaaattc aatctaaaca aaaaggaaac tctcatgttt tattacttac
    11521 aatcccctca ttaaacacaa gaccggttta ttaaatttag cagaagaacc gggcaatatc
    11581 tctcaagcct gcaatgtggt gggtatgagc ctagatacat tctattgcta tcaacaagcg
    11641 gttatttttg gcaactaatt tgcaaaaaaa caataaaatc cgaccgcttg tagagggtta
    11701 atccgccctt agtgaaattt caccgatgcc gaaatagcgg aggttgtcgc cctgttttag
    11761 aatgagttcg ccctgctcgt taatgccttg cgaaatgccg tggatttctt cggcttcggt
    11821 aatgagctta accgctttat ggcggaaaag gtcgaaactt tgccagcgtt cggcataatg
    11881 agcgaagccg acaagcggat aaattttgag atttttttgc aattcatagg ctaaacggca
    11941 aaccaattcg ttgcggttga aatgatattg cgataaatct gcccacgctt gcgtcacaat
    12001 attttcatcc acttttgcca ttcctaggtt cagcccaatg ccgataacca catctaaacc
    12061 attgcgattg gcttgggttt ccaacaaaat gccccccatt tttttgcctt ggtaataaat
    12121 gtcattcggc catttaattt gaatatcttt tacattttga acagtaaggc tttccgcaat
    12181 gatcaaggct accactaaac ttaagggcgg taaatctgcc gcctgctcca tcgggtaatg
    12241 ccataaaatc gagaaataga gattttcact ctcgggcgaa taccaagttc gacctcgtct
    12301 gccacgtccg gcagtttgtt tttcagcgag gcaaaccgtg ccgttttcca aacgttggtg
    12361 atgagtgagc aaatattcat tggttgaatc aatttcatcg aacacaacgg cttgcccgca
    12421 aattaaagca ttttggattt gagcttggtt aagtttcatt atttccccac ttagcgagtg
    12481 attttttcgc tatcgacctc accgttttta ccgataaaac gcacttcagg ctgaatttct
    12541 accccaaatt tttctttcac tctctggcga acggttttgg ctagagccaa gacatcttgc
    12601 cctgttgcat tttctttatt aatcagcact aaggcttgct gggtatgtac cgccgctccg
    12661 ccaatttgta agtctttcag cccggtttgg tcaattaacc aacctgcggc taattttacc
    12721 gttccatcag cttgagggta gtgaggaatg gctgggaatt tggcttgtat tggtgcaaat
    12781 tgttcgtttg aaatcaccgg atttttaaaa aagctgccgg cattgccaaa ttcgtcaggg
    12841 tttggcaatt tggaggagcg aaccgcacag acttcatcaa aaatctgtat tggcgtaacg
    12901 gtttccggat tgagttgggc taatgatccg taacttagca ccggtttcca cgctttggct
    12961 aactttaagc ctaccgagat aatcgcaaat tcatcacggt attggtgttt aaacacactt
    13021 tcacgatagc caaattcgca ctcggatttg gatagcttaa agagttctcc ggaacgtaaa
    13081 tttaacactt ccacgaaatc acatacctgt tcaaattcaa ccccataagc tcctatattt
    13141 tgaatgggag cagagctggc gacacccgga attaacgcca gattttcaat gccggcaata
    13201 ttgtgtgcca acgtccattt aaccaattcg tgccagtttt ccccaccttg aacgtgcaaa
    13261 tagtggaaat gttcgtcttc gtggtgcgta atccctttga gtttatttac taacaccaca
    13321 ccgtcaaaat cctctaaaaa caaaacgtta gagccttgcc cgagaatcaa gattggcagt
    13381 tgagcgttaa aggctttttg ccattcgctc aatagctgtt cgactgtggt aaattcaata
    13441 atttgggtcg cttttgcagg caaatggaaa gtgtgaaaag gggttaagct atgtttcatc
    13501 atcaaattag ttagttaaga agtggaaata acaagcggtg atatttttac aaaattttgc
    13561 aattaagatt ttgggcaggc acaggatctg cccctacatt cagaattacc ttgccccata
    13621 taccacaatg gttttgccgt gagcagaaat taagccttcg tcttccaaca tttttataat
    13681 gcgtccaacg gtttcacggg aacaaccgac catttgcccg atttcttggc gggtaatttt
    13741 gatctgcata ccttccgggt gagtcattgc atccggcatt ttagctaagt tgagcaaggt
    13801 ttgagcaatg cgaccggcaa cgtctaaaaa ggcaagatta ccgacttggc gtgaagtttg
    13861 acgtaaacgg cgagccattt gtgccgataa atacattaaa atatcggggt ttaaatggac
    13921 tagttgtttg aattttttat aggaaatttc cgcaatttca caaggacctt tagtgcggac
    13981 gtaagcggta cgttgttgga ctttttcttc aaataaactt aattcaccga caaactcacc
    14041 tgtgcctaag ttagttaaga gcatctcttt ggtttcatca tctttgatgt aaaccgacac
    14101 tgagccattc acgatgtaga aaagtgattc tgcatcttca ccggcgtgaa tgagtgtata
    14161 tttcgccgga taacgatgga tatgacaatg ggttaaaaac cactccaccg cagggtcgtg
    14221 aatagagggc ggaggagtta cattttccag aggagctgtt gaaagtgtca tatcttgcat
    14281 agtatttcct ataactctta tactaggtta aatttttaga attttaacac gaaattaaag
    14341 gttagcctaa aaaagatttt ttgtctttaa tatcaaggct ttcgtgtaat tccgaccaaa
    14401 caatcaccac tttatctgcc ttaagttgtg tgagtaatcg ttctcttttt tctgccagag
    14461 aaagttccct ttccccgtag tctgtgcctt ctcgtaaaat gaaggaatct aaaatattat
    14521 gtaaggttga ttcttcgagt tcttgccaag gtataatcat aattaatcaa ataaaggctt
    14581 aataaatgcc cactgttttt caaaatgttg gtgttggtta tagctgaaat tagaacgaac
    14641 caagcgtaaa aattgccctt cgcagaaatt cactaaataa ccggctaagg ctctttcgtc
    14701 ttcaaagctc ttgcgttcac gcagtttact aagttgcaaa atattggcaa attgtaactc
    14761 caagttatca aagaattttg ccactcgagc tttgagcaac tcctcttcaa acatcaaggc
    14821 gtgtccggtc aggatgcggg taacgcccgg gtttttgcga gcaaattcta aaatcacata
    14881 taaaattgct cgcaccgaat tggcactgct gtcttggcgt ttattggcgt gaatatagct
    14941 ggtgagggtc tgctcgattt tctcgattaa cgcttcaaac atcttggttt tacttgggaa
    15001 atagcgatac aacgccccct ccgagacccc caccgcagcg gctaaacgct cggtagtgat
    15061 acgctgcatt ccctcttcag aatttaacaa gctgattaac acttccaaca cttgttggcg
    15121 gcgttccacc actgattttt tcggcatttt aattttaggt tctgtcattg ccaatcctat
    15181 tattgcttac ctgaatgccc aaaaccgcct tcgccacggt cggtctgctc aaactcttgc
    15241 acaatgttaa aacttgcctg aaccaccggc acaaagacta actgagcaat acggtcgccc
    15301 acgttcacgg taaaatcagc ttggctacgg ttccaaagcg acactttcag ctcgccttgg
    15361 taatcgctat cgatcaagcc gactaagttg cctagcacga tcccgttttt atgccctaaa
    15421 cctgatcttg gtaagatcac cgctgccaaa ttcggatcgg cgatataaat tgaaatcccc
    15481 gttgggatca acaccgtttc acccgctttt accgtcagcg gctcgttgag catcgcccgt
    15541 agatccaagc ctgccgagcc ttcggtcgca tacgcaggta atggaaattc attgccgata
    15601 cggctatcta aaatttttaa atcaatttgt ttcattcttt ttcctttttg taaattttta
    15661 aaactat
//
LOCUS       DANLYI010000041        14964 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.041, whole genome shotgun sequence.
ACCESSION   DANLYI010000041 DANLYI010000000
VERSION     DANLYI010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 14964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14964
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.041"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            <1..444
                     /locus_tag="PXD81_000921"
     CDS             <1..444
                     /locus_tag="PXD81_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011271796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDL5536967.1"
                     /translation="LNYLGTLLPTQVFAKDMLGKEGANIINISSMNAFTPLTKIPAYS
                     GAKSAISNFTQWLAVYFSKVGIRCNAIAPGFLVSNQNKALLFNADGTPTARANKILTN
                     TPMGRFGQADELVGAILFLMDHHYSSFINGVVLPVDGGFSAYSGV"
     gene            461..1408
                     /locus_tag="PXD81_000922"
     CDS             461..1408
                     /locus_tag="PXD81_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HDL5536968.1"
                     /translation="MKKLAILGECMIELNGEPFGEMHQTYGGDSLNTATYLARVSSPE
                     QIEVRYISALGTDKLSQGMLAAWQVDNINTDFVLRDEHRQPGLYLIQLDKQGERTFLY
                     WRNQSAARYLLQHPGYPKALSALNEVDMVYLSGISLAILPENDRTLLIAQLRELKTQG
                     VKIAFDSNYRPKLWESVAAAQAHYAELLQLVDLALVTFDDEQLLWGDVNEQATFDRLT
                     QYGIQTIVVKQGSLGATVLQNGERTFVPTVAVEKVVDTTSAGDSFNAGFLNGYLLGKD
                     VVTCCQQGNAVAGIVIQHKGAIIAKSATTHLKSQFEQSI"
     gene            1496..2134
                     /locus_tag="PXD81_000923"
     CDS             1496..2134
                     /locus_tag="PXD81_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HDL5536969.1"
                     /translation="MRYTTEQIIEKLRQLKVVPVIALDKAEDILPLAKTLSENGLPVV
                     EITFRSEAAEEAIKLVQANYPEVLIAAGTVLTKEQVLTAKNAGADCIVTPGFNPTIVK
                     YCQVLGLPVTPGVNNPMAIEAALELGIQAVKFFPAEASGGVKMIKALLGPYGNLQIMP
                     TGGISPQNINDYLAIPNVVACGGSWFVEKSLIKEQKWEEIGKLTKEVVELVK"
     gene            2358..3812
                     /locus_tag="PXD81_000924"
     CDS             2358..3812
                     /locus_tag="PXD81_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF560 domain-containing protein"
                     /protein_id="HDL5536970.1"
                     /translation="MKKIVSTLLILTSNLAFAETTPAQLQEKMDNQRMQKNLHTLEKP
                     EPQAKPQAVNAQAHRQSISISSQQLLQQPELLNSAMLTALVTNHTENVQFLLPIYQQL
                     PASKQNSEVVQWAEAVNAKAKQNYSEAIKGYRQLLAKYPENQPIRMQLAVALFENREN
                     EAAEAQFYKLQADKLPQEIANAVNSYLEAIGKQDQWSFQGGLTYLNDPNINNAPNAGT
                     TYGNWTAPKKESAQGVGFHFEADKKWSWGNGFFHEFRLNGNGKYYWNNKKYNEYSLRE
                     SLGLGYQNAKQKITLLPFFEQMWYAGGSSQTESTKRYSNAGGVNARWQYWITPQWQSA
                     ISYEYAEQRYTRRQHLDGNYHFIAPSLYFYPNSRQYWFVGANFNRTSPRDLDDSFIRR
                     GVSIGWGQEWKNGLSTQLSASYARKQYRAPMPIFQITQRNSEYGVQASIWHRALHFKG
                     ITPRLTWQYNKVKSNHSFYSYDKNRVYIEFSHTF"
     gene            complement(3860..6457)
                     /gene="mutS"
                     /locus_tag="PXD81_000925"
     CDS             complement(3860..6457)
                     /gene="mutS"
                     /locus_tag="PXD81_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HDL5536971.1"
                     /translation="MTQDFSTHTPMMQQYLQLKAQNPDILLFYRMGDFYELFYDDAKR
                     AAALLDISLTKRGQSAGEPIPMAGVPYHAVEGYLAKLVALGESVAICEQIGDPATSKG
                     PVERKVVRIVTPGTVSDEALLPERQDNLVAAVYEEKGTFAIATLDMTSGRFLISELAS
                     KEALSAELQRVQPAEILYAEDFSAASILTNYKGLRRRPVWEFELVTAIQLLNRQFGTQ
                     TLAGFGVEKAVVALCAAGCVLHYAQETQRTALPHINAIHLAQHSDTILLDAATRRNLE
                     LTQNLAGGTENTLAAVLDKCVTPMGSRLLKRWIHQPIRDCGKLQARQKAITALIQYER
                     MSDLQPLLQQVGDMERILARVALRTARPRDLTRLRTALAQLPEIAKNSQNLTASIDQL
                     LAQLADFSPLHDLLERAIIENPPQLIRDGGVIAEGYNAELDEWRQLSDGATQYLENLE
                     IRERESTGIDSLKIGFNAVHGYYIQISQGQAHKAPIHYVRRQTLKNAERYIIPELKTY
                     EDKVLKAKGASLALEKQLYDELFDVLMPRLGELQLAAMMLSELDVLTNLAERADSLGY
                     VCPSFSPMRMVNIKGGRHPVVEQVLTEPFIANPVFLNSQRHLLIVTGPNMGGKSTYMR
                     QIALITLMAYIGSFVPAESAEIGPIDRIFTRIGASDDLASGRSTFMVEMTEMANILHQ
                     ATENSLVLIDEIGRGTSTYDGLSLAWACAEWLAKKTQSLTLFATHYFELTGLPNQLKG
                     VANVHLDAREHQDSIVFMHSVQEGAASKSYGLAVAALAGVPKQVIQLAKQRLAHLEEI
                     SHQTKQANEHPQADLLFSDGFDLQKNEAIQPLVVENEVEKALKALQPDELTPRQALDE
                     LYRLKKLLG"
     gene            6607..7170
                     /locus_tag="PXD81_000926"
     CDS             6607..7170
                     /locus_tag="PXD81_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005613140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5358 domain-containing protein"
                     /protein_id="HDL5536972.1"
                     /translation="MLRKIVLLSTGLLLAACSSNEPQQPEAFPGEFANADYVLSEENA
                     QKWVIASEQAKQCIYPNLTRIQQEHFSKEDSYIFSQYVFFYPLEEIIGEQYVKIIQND
                     EKSMGYAQYLFKKLKNNEAVEALPKKQCATLRTNAKNDLAVVKGQYQSGMIEETNSQE
                     GKSGDKVATNDNKFFFDIIKWGSMLLL"
     gene            7344..7973
                     /locus_tag="PXD81_000927"
     CDS             7344..7973
                     /locus_tag="PXD81_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HDL5536973.1"
                     /translation="MAVSATSFAQTTQPTAAEQMNAVKASVAEKATVAKEATKEKAAE
                     LKAGSAEKMAIAKAKTESFKDSAKNKMDAVKATTAEKTDVIKAKAASASDTMKVKAQE
                     AKAATAEKAATAHETLNSAKTATAEKAASMKAAATEKMSAVKKSQKVNINTANEEQLQ
                     SLTGIGEAKAKAIVEYRKKHGKIKNAAELANIPGIGDATIEKVAPYLTF"
     gene            8121..8750
                     /locus_tag="PXD81_000928"
     CDS             8121..8750
                     /locus_tag="PXD81_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007526112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HDL5536974.1"
                     /translation="MALDFNSVGLDSVEAKGGYGIGLQIGQQLLGSGMDVNAETVARG
                     IYDVLNSNEPAIDINEVTAALQELGQRAEAAQAEAFKAIDAENKAFLEENKKANGVIV
                     TDSGLQYEILTEGTGEKPTATSTVRVHYTGSLIDGTVFDSSVKRGQPAEFPVNGVIRG
                     WTEALQLMPVGSKWRLTIPQELAYGERGAEASIPPFATLVFEVELLDIL"
     gene            8816..9331
                     /locus_tag="PXD81_000929"
     CDS             8816..9331
                     /locus_tag="PXD81_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein disulfide oxidoreductase"
                     /protein_id="HDL5536975.1"
                     /translation="MQKKSLISRLLKNIFLYGSLFIIISLVVDWYRSPSAPAQFEQTV
                     HYDIQHQPKVIAQLSHEKPMLLYFWGSWCHYCQVVSPNIQQLANDGTEVLGVALKSGD
                     ENDVQAYLNENGYTFATLNDPSGDFSRGWNIQATPTILIIKDGKIAHHTTGYTSYLSL
                     KFRLWLADVLY"
     gene            complement(9379..10309)
                     /locus_tag="PXD81_000930"
                     /pseudo
     CDS             complement(9379..10309)
                     /locus_tag="PXD81_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015433453.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(10368..11018)
                     /locus_tag="PXD81_000931"
     CDS             complement(10368..11018)
                     /locus_tag="PXD81_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595-like element ISMha4 family transposase"
                     /protein_id="HDL5536976.1"
                     /translation="MRKSRLSQYKQNKLIEQFVAGVTARTASELVNVNKNTAAYYFHR
                     LRLLIYQHSPHLEMFEGEIEVDESYLGGHRKGKRGRGAAGKTAVFGLLKRDGKVYTVV
                     VPNTQFATLLPIIREKVKPDSIVYTDFYRSYDVLDVSEFNHFRINHSTHFAENQNHIN
                     GIENFGSQAKRHLRKFNGIPKVHFELYLKECEWRFNHSDLKSQISFLKQLVKGSLG"
     gene            11347..12705
                     /gene="mnmE"
                     /locus_tag="PXD81_000932"
     CDS             11347..12705
                     /gene="mnmE"
                     /locus_tag="PXD81_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007523948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HDL5536977.1"
                     /translation="MKDTIVAQATPIGRGGVGILRVSGPLAETVAQAVLGKTLPPRIA
                     NYLPFKDEDGTVLDQGIALFFKAPNSFTGEDVLELQGHGGQVILDLLLKRILQVKGLR
                     IARAGEFSEQAFLNDKLDLAQAEAIADLIDATSEQAARSALKSLQGEFSNKINALVDS
                     VIYLRTYVEAAIDFPDEEIDFLADGKIEAKLNEIIAQLDGVRREAKQGSILREGMKVV
                     IAGRPNAGKSSLLNALAGREAAIVTNIAGTTRDVLREHIHIDGMPLHIIDTAGLRDAS
                     DEVERIGIQRAWEEIAQADHVLLMIDSTEQQADQFRQEWAEFLAKLPANMPVTVIRNK
                     VDLSGEQEGLIQVDDFTMIRLSAQTKVGVDLLREHLKKSMGYQSSTEGGFLARRRHLQ
                     ALETAAEHLERGHIQLTQFLAGELLAEELRMVQNALSEITGQFTSDDLLGNIFSSFCI
                     GK"
     gene            12706..13971
                     /gene="tilS"
                     /locus_tag="PXD81_000933"
     CDS             12706..13971
                     /gene="tilS"
                     /locus_tag="PXD81_000933"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HDL5536978.1"
                     /translation="MALFNRFQTQCATHLPNQTAFLIGLSGGVDSVVLLHLFARTSFH
                     VRAIYVHHGLSPNADDWAAFCEQYCKRLNIPFILQKVRVDSSNGVENGAREARYQAIQ
                     QQLKPNEVFATAHHLDDQAETFLLALKRGSGVKGLSAMQAVTFLQNFTVFRPLLTFTK
                     SDLIGYAVQHQLGWIEDESNADNRYDRNFLRNSILPLLNQRWQHFSQMVARSAQHCAE
                     QQALIEELLSNELKSRTGEKQQLNINGFGQFSLAKQQQLSRLWLEQNGVRMPSQAQLQ
                     AVISELIFAKADKNPQVKVGENIIRRYQQAIYVTEEIADIPPFEVVLPTETKVELPYQ
                     LGTIIRQQQEIICKKNGKNHRLQLPQELAQSTISIKIGQLGKVKCYGKAQREEMKKIW
                     QNHGIPVWERNRTPLIFWQDELVGLLPIL"
     gene            complement(13961..14854)
                     /gene="xerD"
                     /locus_tag="PXD81_000934"
     CDS             complement(13961..14854)
                     /gene="xerD"
                     /locus_tag="PXD81_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005605467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HDL5536979.1"
                     /translation="MAELDPIIEQFLDTLWQEHGLSENTVASYRSDLERFSDYFSEPK
                     AFLSLDHIDLQGFLGERLEQGYKATSSARMLSCLRKFFRFLCLEHYRQDDPTLTLSSP
                     KRGASLPKSLSEDQVMDLLDAPNTLDPIELRDKAMLELLYATGLRVTELVSLSMDNIS
                     LKQGVVRIIGKGDKERLVPLGEEANYWIQEFFQYGRPILLNNQQSDVVFPSRRGQQMT
                     RQTFWHRIKHYAVLAGIDADKLSPHVLRHAFATHLVNHGADLRVVQMLLGHSDLSTTQ
                     IYTQVAKARLKSLHQQFHPRG"
BASE COUNT         4449 a         3448 c         3225 g         3842 t
ORIGIN      
        1 ttaaattact taggcacact attaccaaca caggttttcg caaaagatat gttgggtaaa
       61 gagggcgcta atatcattaa tatatccagt atgaatgcct ttactccatt aactaaaatc
      121 cctgcctatt ctggagcaaa atctgcaatc agtaacttta cacaatggtt agcagtttat
      181 ttctcaaaag taggtatccg ctgtaatgct attgcaccgg gatttttagt gagtaatcaa
      241 aataaagctc tactatttaa cgccgatggt acgccaacag ctagagctaa taaaatcctc
      301 acgaataccc ctatgggacg tttcggacaa gccgatgaat tggtaggtgc aatcctattt
      361 ttaatggatc accattattc cagctttatc aatggcgttg tattacctgt tgatggcgga
      421 ttctctgctt atagcggcgt gtaacttaga aggtattcct atgaaaaaac tcgccattct
      481 cggtgaatgt atgattgagt taaacgggga gccgtttggg gagatgcacc aaacctatgg
      541 tggggattcg ctcaacaccg ctacctattt ggctcgggtg agttcgcctg aacagattga
      601 ggtgcgttat atttccgctc tcggcacgga taagctcagt cagggaatgt tagcggcgtg
      661 gcaggtggat aatatcaaca cggattttgt gttgcgtgac gagcaccgcc agccggggct
      721 gtatctgatt cagttggata aacagggcga gcgaacgttc ctctattggc gaaatcaatc
      781 tgccgcccgt tatttgttgc aacaccccgg ctatcccaaa gcactctcgg cactaaacga
      841 ggtagatatg gtttacttga gcggtatcag ccttgccatc ttgccggaaa acgaccgcac
      901 tttgctgatt gcacagttgc gtgagctcaa aacacaaggg gtgaaaatcg cctttgacag
      961 caattaccgg ccgaaactgt gggaaagtgt ggctgctgcc caagctcact atgccgagct
     1021 gttgcaatta gtcgatttag ctctcgtgac ctttgatgac gagcaactgc tttggggcga
     1081 tgtgaatgag caagccacct ttgaccgtct cactcaatat ggcattcaga ccattgtggt
     1141 gaaacaaggc tctctggggg caacggtgtt gcaaaacggg gagcgtactt ttgtgccaac
     1201 tgttgcggta gagaaggtgg tcgataccac ttccgccggc gattccttta atgccggctt
     1261 ccttaacggt tacttattgg gtaaagatgt cgttacctgt tgccaacaag gaaatgccgt
     1321 tgccggtatc gtcattcagc acaaaggggc gattattgcc aaatccgcca ctactcattt
     1381 gaaatcgcag tttgaacaat ccatttagac caacaatagc acaacgacaa gcggttaaat
     1441 ttgcaaaatt ttttacaaaa gcgaccgctt gcaatacaac ataatgaggc tagaaatgcg
     1501 ttacaccacc gaacaaatca ttgaaaaatt aagacaactg aaagttgttc ccgtgattgc
     1561 tttggataaa gcagaagata ttttaccgct ggcgaaaacc ctttccgaaa acggtttgcc
     1621 ggtggtggaa atcactttcc gctcggaagc agctgaagag gcaattaaat tagtgcaagc
     1681 caactatccg gaagtattga ttgcagcagg cacagtgctg accaaagagc aagtcttaac
     1741 tgccaaaaac gctggggcgg attgcattgt gactccgggt tttaatccga ctatcgttaa
     1801 gtattgccaa gtgttaggtt tgcctgttac accgggtgtg aacaacccga tggcgattga
     1861 agccgccctc gaacttggca tacaagcagt taaatttttc cctgccgaag ccagtggcgg
     1921 cgtgaaaatg attaaagccc tgctcggccc ttacggcaac ttacagatta tgccgaccgg
     1981 cggcatctcg ccacaaaaca tcaacgacta cctcgccatt cccaacgtag tcgcctgcgg
     2041 cggctcttgg tttgtggaga aatccctgat taaggaacag aagtgggagg agattgggaa
     2101 attgacgaag gaagtggttg aattggtgaa ataaccattt aaagttccaa gcaatgagct
     2161 attttctacc caaaatggat ctctttcctc ttatttacaa agcataacaa atattgcata
     2221 ctttatttag tagcatatag taggtatatt taaagctata tatttaactt aatgaaaaat
     2281 atagcaaaac aacctcaaaa ctcttcacta aaggctattg aaaaatagcc ttttctattc
     2341 aaataaaaga tagctttatg aaaaaaatag tctcaacgct tttaatcctt acctcaaacc
     2401 tggcatttgc ggaaaccaca ccggctcaac ttcaagaaaa aatggataac cagcgaatgc
     2461 agaaaaacct acatacatta gaaaagcctg aacctcaagc caaaccacaa gcggttaatg
     2521 cacaggctca ccggcaatcg atctctattt caagccaaca gctgcttcaa cagcctgagt
     2581 tgctcaactc ggcaatgcta actgcgttgg tcactaacca cactgagaat gttcaattct
     2641 tactgcctat ttatcaacaa ttacccgcca gtaaacaaaa cagcgaagtt gtacaatggg
     2701 cggaggcagt taatgccaaa gcaaaacaaa attattcaga ggcaataaaa ggctaccgtc
     2761 agctactggc aaaataccct gaaaatcagc caatccgtat gcaattagcg gttgcattgt
     2821 ttgaaaatcg ggaaaatgaa gccgcagagg ctcaatttta taagttacaa gcggacaaat
     2881 taccgcaaga aattgcaaat gcggtcaata gctatttgga ggcgattggc aagcaagatc
     2941 aatggagttt tcaaggcggc ttaacctatt tgaatgaccc gaatatcaac aatgctccaa
     3001 atgcaggcac gacctatggc aactggactg caccgaaaaa agaatccgcc caaggggtcg
     3061 gctttcactt tgaggcggat aaaaagtggt cttggggcaa tggtttcttc cacgagtttc
     3121 gtttaaacgg aaacggcaaa tattactgga ataacaaaaa atataacgaa tattcgctca
     3181 gagaaagcct cggcttgggt tatcaaaatg caaaacaaaa aatcaccttg ttgccgtttt
     3241 ttgagcagat gtggtatgcc ggtggctcaa gccaaaccga aagcaccaaa cgctactcca
     3301 acgcaggcgg tgtgaatgcc cgctggcaat attggattac tccacagtgg caatctgcaa
     3361 tcagctacga atatgcggaa cagcgttaca ctcgccgcca acatttagac ggcaattatc
     3421 attttattgc cccgagcctc tatttttacc ctaacagccg gcaatattgg tttgtggggg
     3481 cgaattttaa ccgcaccagc ccccgtgatt tagatgatag ctttattcgc cgaggtgttt
     3541 ctattggttg gggacaagag tggaaaaacg ggctttcaac ccaattaagt gccagctacg
     3601 cccgcaaaca ataccgagcc ccaatgccga ttttccaaat tacacaacgt aacagtgaat
     3661 atggtgtgca agccagcatc tggcacagag ccttgcattt taagggtatt acccctcgct
     3721 taacgtggca atataacaag gtaaaaagca atcatagctt ctatagttat gacaaaaacc
     3781 gtgtctatat tgagttttca catacgttct aaacaaaaac gccccttaaa cggggcgatt
     3841 tcttagttat tttttcaatt taccccaaca attttttcaa gcgatacaac tcgtccaaag
     3901 cctgtcttgg ggtaagttca tcaggctgaa gggctttcag ggctttttca acttcatttt
     3961 ctactacaag cggttgaatt gcttcatttt tttgcaaatc aaaaccatcg gaaaacaaca
     4021 aatccgcctg tggatgttcg tttgcttgtt tggtttggtg ggaaatttcc tccaaatgtg
     4081 ccaaacgctg ctttgccagt tgaatcactt gctttggcac acccgccaag gctgccaccg
     4141 ccaagccgta acttttgctg gcggccccct cttgcaccga gtgcataaac acaatactgt
     4201 cttgatgttc acgcgcgtct aaatgcacat ttgccacccc tttgagctga ttcggcaagc
     4261 cggtaagttc aaaatagtgg gttgcaaaca aagtgagtga ctgggttttc ttcgccagcc
     4321 attcggcaca cgcccacgcc agtgataagc cgtcataggt agaagtgcct cgcccgattt
     4381 catcaatcag cacaaggctg ttttcggtgg cttgatgcag aatattcgcc atttcggtca
     4441 tttccaccat aaaggttgaa cgccctgaag ctaaatcatc gcttgcccca attcgggtga
     4501 aaatacggtc aatcgggccg atttccgcac tttctgccgg cacaaaactg ccgatatacg
     4561 ccatcaatgt aatgagggca atttgccgca tataggtgct tttaccgccc atattcgggc
     4621 cggtcacaat caataaatgc cgttgggaat ttaaaaacac cggattggca ataaacggct
     4681 cggttaaaac ctgctccacc accgggtgtc tgccgccttt aatattcacc attcgcatcg
     4741 ggctaaagct cgggcaaaca tagcctaaac tatcagctcg ctcggcaagg ttggttaaga
     4801 catcaagttc agacagcatc atcgccgcta attgcaattc gcctaagcga ggcattaaca
     4861 catcaaacag ttcatcataa agctgttttt caagggcaag cgatgcccct tttgctttca
     4921 gcactttgtc ttcgtaagtt ttcagctcag ggataatata ccgctccgca tttttgagcg
     4981 tttggcggcg aacatagtga atcggggctt tgtgtgcttg cccttggctg atctgaatgt
     5041 aatagccgtg aaccgcatta aagccgattt tcagcgaatc aatgccggtg ctttcacgtt
     5101 ctctgatctc caaattttcc agatattgcg ttgccccgtc cgaaagttgc cgccattcgt
     5161 caagctcggc attataacct tctgcaatca ccccaccatc acgaatgagt tgtggtgggt
     5221 tttcgataat cgcccgctcc agcaaatcgt gtaacgggct gaaatccgcc agttgagcca
     5281 aaagctgatc gatactagcg gtcagatttt gtgaattttt tgcaatttcc ggtaactgag
     5341 ccaatgcagt gcgtagtcgg gtgaggtcac gagggcgagc cgtacgcaac gccactcggg
     5401 cgagaatccg ctccatatcg ccgacttgtt gtaataacgg ctgtaaatcc gacattcgct
     5461 catattgtat caatgctgtt atcgcctttt gtcgggcttg taatttacca caatctcgaa
     5521 tcggctgatg aatccaacgc ttgagcagac ggctccccat tggggtcaca catttatcta
     5581 acaccgccgc cagcgtgttt tccgtgccac ccgctaagtt ttgggtcagc tctaaattgc
     5641 gacgagtagc tgcatcgagc aaaatagtat cgctatgttg tgccaaatga attgcgttaa
     5701 tgtgcggtaa agcggtgcgt tgcgtttctt gggcataatg caagacacag ccggcagcac
     5761 aaagtgccac caccgctttt tccaccccaa atccggcaag tgtttgcgtg ccgaattggc
     5821 gatttagcag ctgaatcgcc gttaccagct caaattccca caccggacgg cggcgtaacc
     5881 ctttgtaatt cgttaaaatt gaggcagcgg aaaaatcttc cgcatacaga atttctgccg
     5941 gttgtacacg ttgtaactcg gcggatagag cctctttgct agcaagttca ctgattaaaa
     6001 accgcccgga ggtcatatct agcgtagcaa tcgcaaatgt gcctttttct tcgtaaactg
     6061 ccgccaccaa gttatcttgg cgttcgggta acaatgcctc atcgctaact gttcccggcg
     6121 taacaatccg taccactttg cgttctaccg gcccttttga ggttgctggg tcgccaatct
     6181 gttcacaaat tgctaccgat tcccccaacg ccaccaattt cgccaaatac ccctccactg
     6241 cgtgataagg cacacccgcc atcggaatcg gctcaccggc agattgccct cgcttggtga
     6301 gcgaaatatc caacaatgct gccgctcgtt tcgcatcatc ataaaacagc tcataaaaat
     6361 cccccattcg gtaaaacaat agtatgtccg gattttgagc tttgagttgc aaatattgtt
     6421 gcatcattgg cgtatgggtg gagaaatctt gtgtcatcag tctatttatt gtgagaaatc
     6481 attgctgata gtttaacaga ttcagccaag ggataaaaac aagcggtcat ttttcgctga
     6541 aaatttgcaa tggtcagccg ctgttttttt gttacaatgc accaaatttt taaaacagag
     6601 gtcagaatgt taagaaaaat agtgctactt tctacaggct tattattggc ggcttgtagc
     6661 agcaatgaac cgcaacaacc ggaagcattc ccgggcgaat ttgccaatgc cgattatgtg
     6721 ctttcggaag aaaatgctca aaaatgggta attgccagcg aacaggcaaa acagtgtatc
     6781 taccccaatt taacccgtat tcagcaagag catttttcca aagaagattc ttatattttc
     6841 tcacaatatg tatttttcta tccgcttgaa gagattatcg gggagcagta tgtaaaaatc
     6901 attcaaaatg atgaaaaatc aatgggttac gctcaatatt tattcaaaaa actgaaaaat
     6961 aacgaagcgg ttgaagcgtt accgaaaaaa caatgtgcga ccttacgcac caatgcaaaa
     7021 aatgatttgg cagtggtaaa agggcagtat caaagcggta tgatcgaaga aaccaatagc
     7081 caagaaggga aatcaggcga taaagttgcc accaatgata ataaattctt cttcgatatt
     7141 atcaaatggg gcagtatgtt acttctctag tgagcctcaa tactcggttg ttttaaccac
     7201 aaccgagcca tttataccta ccgattgaac ttttatctaa agcctcagtc ttaaaataca
     7261 gtttttttca attattttcc ctcttttata tttaaggatt ttcaataaaa gcattaaaaa
     7321 cattattagc attgggcgtg gcaatggctg tttcagctac ttcatttgca caaaccactc
     7381 aacctactgc ggctgaacaa atgaacgcag ttaaagcctc tgttgccgaa aaagcaactg
     7441 tggctaaaga agcaacaaaa gagaaagcag cggagttaaa agccggttct gcggaaaaaa
     7501 tggcgatagc aaaagccaaa accgaatcat ttaaagattc cgctaaaaac aaaatggacg
     7561 cagtaaaagc caccacagca gaaaaaacgg acgtgattaa agcgaaagcc gcatctgcaa
     7621 gcgacacaat gaaagtgaaa gctcaggaag caaaagccgc caccgcagag aaagctgcaa
     7681 ctgctcatga aaccttaaat tcagccaaaa ccgcaaccgc tgaaaaagcg gcctcaatga
     7741 aagcagctgc aacagaaaaa atgtcagcag tgaaaaaatc acaaaaagtg aatatcaaca
     7801 ctgccaacga agagcaatta caatcgctta ccggcattgg tgaagcaaaa gccaaagcga
     7861 ttgtagaata ccgcaaaaaa cacggtaaaa tcaaaaatgc agcagaactg gcgaacattc
     7921 ccggcattgg cgatgcaacc attgagaaag ttgctccata tttaactttc taatgttctg
     7981 caagcggtta ttttgttgca aaattttgca gaaaaataac cgcttgttca tttcacacct
     8041 tgtattctta aatttcaccc ccattaagtg aatcgtattt ctttcaatac agtattcaat
     8101 aaactcaaaa ggaacagaaa atggcattag atttcaattc agttggatta gattcagtag
     8161 aagcaaaagg cggttacggc atcggtttac aaattggtca gcaactttta ggcagcggaa
     8221 tggacgtaaa cgcagaaacc gttgcccgtg gtatttatga tgtgttaaac agcaatgagc
     8281 cggcaatcga catcaatgaa gtgaccgctg cattacaaga attaggtcag cgtgcggaag
     8341 cggctcaagc agaagcattc aaagcgattg atgccgaaaa caaagcgttc ttggaagaga
     8401 ataaaaaagc aaacggtgta atcgttaccg acagcggttt acaatatgaa attttaaccg
     8461 aaggcacagg cgaaaaaccg actgcgactt caaccgtgcg tgttcactac accggttcat
     8521 taattgacgg caccgtgttt gacagctcgg taaaacgcgg tcagccggca gaattcccgg
     8581 taaacggagt aattcgtggt tggacagaag cattgcaatt aatgccggtt ggctcaaaat
     8641 ggcgtttaac cattccacaa gaattagcct acggcgaacg tggtgcagaa gcatcaattc
     8701 caccatttgc gaccttagtg tttgaagtgg aattgcttga tattctctaa tcaaactcaa
     8761 ataaaaaacc tgtggaggca tttcacaggt ttttgctatt taagataact atattatgca
     8821 gaaaaaatca ctcatatctc gcttacttaa aaatattttc ctctacggca gcctgtttat
     8881 cattattagc ctggtggttg attggtatcg ctcgccctcc gctccggcac aatttgagca
     8941 aaccgtgcat tatgatattc aacaccagcc gaaagtaatc gcccaattaa gccacgagaa
     9001 gccgatgcta ctctattttt ggggaagttg gtgccactat tgccaagtgg tttcgccaaa
     9061 tatccaacaa ttagcgaatg acggcacgga agttttaggt gttgccctga aatccggcga
     9121 tgaaaatgac gtacaagcct atctcaatga aaatggctac actttcgcta ctttgaatga
     9181 cccaagcggt gatttctctc gaggttggaa cattcaagcc accccaacca tcttgattat
     9241 caaagacggt aaaatcgccc accacaccac cggctacact agctatttga gcttaaaatt
     9301 cagactatgg ctggcagacg tgttgtatta aacgattcct ttcatttgca aaaaaatcca
     9361 ctaaatccga ccgcttgttt acaccaaaaa gacaagcggt actaaattca ttgtcatcat
     9421 tgtggtgatc gataaaatca gcctcgatga gccaaaaata aagcctcggt ttttgccgtt
     9481 gtcgaatttc accagtaaat ttgccattgc tgtgagggcg tacacttcca ccaccgtaaa
     9541 aatcacggtg taaatatagg cagcggtcag agttgcgact tttaccgcaa taaaccaagg
     9601 ggcaataatc gccaccgcta ataacggcga aatccaaatt aattgctgtt tggtgaggtt
     9661 gattttacga gaaaaagtcg attgcaaact gacggttaaa acaccgttga tgaataacgc
     9721 aatcggcgaa tgctccggtg cgtgcaggac attgtcgaat aaatacggaa aaatcactca
     9781 gcggcacaaa tagcattaag tgagcgaaca aaaactcttt gttgatgatt tccttaattt
     9841 gtgcgaaacg aaatttcacg ccgccattca cgccttccac cgaataaaac ggcttgacca
     9901 taaacgcaaa caagccggct tccaaaataa agcagatcca aatcagcgtt tcaatcatct
     9961 caaacctaat aaacggaata ataatcagcg gggcgagcat tgaagacata ctggtgactt
    10021 ttagatattt gccttgtaaa cgggttttag cagcatattc atcgccggca atcgccagta
    10081 aacaggctct ggcattagtg ccgaataagc aactccccaa cccaaagcaa gtggttgcca
    10141 acaataaaat gaggtaatta tccgctgcta aaaacagcat ataggcaacc acatctaata
    10201 aacagcccaa taacatcatt tttgccaagc cgtagcgatc cccccaaatg ccggctaaaa
    10261 ttgccaatgc ctgattacaa aacaacagca atgaaaggaa aaaagcgatt tgggcgttat
    10321 ctagcccttt ctcttttaaa taaaagaaaa ggggctgtcc tagataacta acctaaacta
    10381 cccttaacca attgttttaa aaaggatatt tgagatttta agtcactgtg attaaaacgc
    10441 cattcgcatt ctttcaaata cagctcaaaa tgcactttgg gaatgccgtt aaacttacgc
    10501 aaatggcgtt ttgcttggct cccaaaattc tcaattccat taatgtgatt ttgattttca
    10561 gcgaagtgtg tgctgtgatt aatgcgaaaa tggttaaatt cactcacatc aagcacatca
    10621 taacttcgat agaaatcggt gtaaacaata ctatcgggtt tcaccttttc ccgaataatc
    10681 ggcaaaagtg tggcaaattg cgtgtttggt actaccacgg tataaacctt tccgtcccgc
    10741 tttaaaagcc cgaacacggc ggttttgcct gctgcaccac gtccacgttt acctttacga
    10801 tgaccgccta aataactctc atcaacctca atttctccct caaacatttc caaatgaggg
    10861 ctatgttgat agataagcaa ccgcaagcga tggaagtaat acgccgccgt gtttttgttt
    10921 acattgacca actcagatgc tgttcgggct gttacccctg caacaaactg ctcaatgagt
    10981 ttgttttgtt tgtactgact tagacgactt tttctcattt gggtatctta acctaaatgg
    11041 agtttttagt cgttatctag gacagcccca aagaaaaaca cgctttgcat tgaaaaaaag
    11101 gcggtggtgg aaataaaacg gcgtaggagt aatagttttt ctaaagacat tcagaatggt
    11161 tgaattggtt aaataaagag cggttattct accttttaag tcaaccccaa tcaatcggct
    11221 tttgttttac ctttggcgtg ggtttcggta tgataagcga tatgtagtgg cgggtcctgt
    11281 gcccgcctaa ataccaatga taacttgcgg gcgagcacag gactcgcccc tacgataaac
    11341 aagaaaatga aagacaccat tgttgctcaa gcaacaccga ttggcagagg cggtgtgggg
    11401 attctgcgag tttccggtcc gcttgccgaa accgttgctc aagccgtatt aggcaaaaca
    11461 ttgccacccc gtatcgcaaa ttatttgccg tttaaagacg aagatggcac tgtgttggat
    11521 caaggcatcg ccttattttt taaagctccc aactccttta ccggcgaaga tgtgcttgaa
    11581 cttcaaggac acggtgggca ggttatttta gatttactgc tcaaacgcat tttacaagtt
    11641 aaggggctac gcattgcccg agcaggtgaa ttttccgaac aggcattttt aaacgataag
    11701 ctcgaccttg cccaagctga agcgattgcg gatttaatcg acgccaccag cgagcaagcc
    11761 gctcgttcag ccctgaaatc actacagggt gaattttcta ataaaatcaa tgcgttagtt
    11821 gattcagtaa tttatttacg cacttatgtg gaagcggcga ttgacttccc cgatgaggaa
    11881 attgactttc tggcagacgg taaaatcgag gcaaaattaa atgagattat cgctcaatta
    11941 gacggtgttc gccgtgaagc gaaacaaggc tcgattctgc gtgaaggaat gaaagtagtg
    12001 atcgccggtc gcccgaatgc agggaaatca agcttgctta acgccttagc cggacgagag
    12061 gcggcgattg taaccaatat tgctggcaca acccgtgatg tgttgcgtga acatattcac
    12121 attgacggaa tgccgttgca tattatcgac accgccggtc tccgtgatgc cagtgatgag
    12181 gtggaacgta tcggcattca gcgtgcttgg gaagaaatcg cccaagccga ccacgtttta
    12241 ttaatgattg acagcaccga gcaacaagcc gatcagttcc gacaagagtg ggcggaattt
    12301 ctagccaaac tgccggcaaa tatgcctgtg accgtaattc gcaacaaagt cgatttatcc
    12361 ggcgagcagg aaggcttaat tcaggtggac gatttcacga tgattcgcct ctctgcccaa
    12421 accaaagtcg gcgttgattt attgcgtgaa cacttgaaga aatcaatggg ctaccaaagt
    12481 tctacggaag gcggattctt ggctcgccgc cgtcatctgc aagcattgga aaccgctgcc
    12541 gagcatttag agcgagggca tatccagctt actcaattct tagccggcga attgttagcg
    12601 gaagagttgc gtatggtgca aaatgcctta agtgaaatta ccggccagtt cacctctgat
    12661 gacttattag gcaacatttt cagctcattt tgcatcggta aataaatggc actttttaac
    12721 cgctttcaaa cccagtgtgc aacgcatttg ccgaaccaaa ccgccttttt aatcggctta
    12781 agcggtgggg tggattcggt ggtactactc cacctttttg cccgcacctc atttcacgtt
    12841 agggcaattt atgtccacca cgggctaagc ccaaatgcgg acgactgggc agccttttgc
    12901 gaacaatatt gcaagcggtt aaatattccc tttattttac aaaaggtcag agtggatagc
    12961 tcaaacggcg ttgaaaacgg ggcgagagaa gctcgttacc aagccattca acaacagctt
    13021 aagcccaatg aagtgttcgc caccgcccac catttagatg accaagccga aacttttttg
    13081 ctcgccctca agcgaggcag tggagtcaaa ggattatcgg cgatgcaagc ggtcactttt
    13141 ttgcaaaatt ttaccgtttt tcgaccgcta ctcactttta ctaagtcaga tttgataggt
    13201 tatgccgttc aacatcagct aggttggatt gaggacgaaa gcaatgccga taaccgttac
    13261 gaccgtaatt tccttcgcaa tagcatcttg ccactactca atcaacgttg gcagcacttc
    13321 agccaaatgg ttgcccgttc cgctcagcat tgtgcagaac agcaagcctt gatagaagag
    13381 ctattaagca atgaactcaa gtcaagaaca ggggaaaaac aacaactcaa cattaacggc
    13441 tttgggcaat tttcacttgc caagcaacag caacttagcc gcctttggct ggaacaaaac
    13501 ggagtgagaa tgccaagcca agcccagcta caagcggtca tttctgaact gatttttgca
    13561 aaagccgata aaaacccaca ggttaaagtc ggcgaaaata ttatcagacg ctatcaacaa
    13621 gctatttacg ttaccgaaga gatcgccgac attccgccgt ttgaggtagt acttcccaca
    13681 gaaaccaaag tggaattacc ctatcagctc ggtactatta ttcgccaaca acaagagatc
    13741 atttgcaaaa aaaacgggaa aaatcaccgc ttgcaactgc cccaagagct ggctcaatca
    13801 actatttcta ttaaaatcgg gcaactaggc aaagtaaaat gctacggcaa ggctcaacgg
    13861 gaagaaatga aaaagatttg gcaaaatcac ggcatacctg tttgggaacg caaccgtaca
    13921 ccgctgattt tttggcaaga cgaattagtt ggcctattac ctatcctcta gggtgaaatt
    13981 gctgatgaag cgatttcaaa cgggcttttg ccacttgagt gtaaatctgg gtggtcgata
    14041 aatcactatg ccccagcaac atctgtacca ctcgcaaatc agccccatga ttgaccaaat
    14101 gggtcgcaaa ggcgtggcga agcacgtgag gcgagagttt atctgcatca atacctgcaa
    14161 gcacggcata gtgcttaatc cgatgccaaa acgtctgcct tgtcatctgc tgacctcgcc
    14221 ggctcggaaa tacaacatca gactgctgat tattcagcaa aatagggcga ccgtactgga
    14281 aaaactcctg tatccaataa ttcgcctcct cgcccaatgg cactaaccgc tctttatccc
    14341 ctttaccgat aattctcacc accccttgtt tgaggctgat attatccatc gaaagcgaca
    14401 ccagctccgt tacccgcaga ccggtggcat acagcagctc cagcattgct ttatcccgta
    14461 actctatcgg atctaacgta ttcggggcat ctagcaaatc cattacctga tcttcactta
    14521 aggatttggg taaagaagca ccccgtttag gggaagaaag ggttaaagtc gggtcatctt
    14581 ggcgataatg ctcaaggcat aaaaaacgga aaaatttcct taaacaactc aacattctgg
    14641 cagagcttgt cgccttatag ccttgctcca aacgctcgcc gagaaaacct tgtaagtcga
    14701 tatgatcgag agacagaaat gctttaggtt cagaaaaata atcggaaaaa cgctctaagt
    14761 cagaacgata agaggcaacg gtattttcag acaaaccgtg ttcttgccat agtgtatcga
    14821 gaaattgttc aataataggg tcaagctcag ccatacaagc gggtatattt tcaggaaatt
    14881 ttgcaaattt taacagaatt aaatctataa aaatacaaaa gcgatagaaa cgaaaaaacc
    14941 gattagctta tgctaatcgg ttta
//
LOCUS       DANLYI010000042        14851 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.042, whole genome shotgun sequence.
ACCESSION   DANLYI010000042 DANLYI010000000
VERSION     DANLYI010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 14851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14851
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.042"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            225..656
                     /locus_tag="PXD81_000935"
     CDS             225..656
                     /locus_tag="PXD81_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3577 domain-containing protein"
                     /protein_id="HDL5536980.1"
                     /translation="MTTQTSTQKNYFNLHTTGIGYLNDIRLVEPKKGNPFYACRIAAL
                     VGESDSPEYRYFDMNVVGKETETLIKRCQKAVSEEKKVLVSFVMADLWFDTFTYSTDS
                     KFHKKGDTGVSVKGRLIRIKMIKIDGEIAYQEKPKSDEQSE"
     gene            674..1666
                     /locus_tag="PXD81_000936"
     CDS             674..1666
                     /locus_tag="PXD81_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005588345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL5536981.1"
                     /translation="MLCWALLLDVQDLRVTFKTPDGDVTAVNDLTFSLNAGETLGIVG
                     ESGSGKSQTAFALMGLLADNGRISGSAKFNGQEILNLPETQLNRLRAEQIAMIFQDPM
                     TSLNPYMKIGEQLMEVLMLHKGMDHKTAFEESVRMLDAVKMPESRKRMNMYPHEFSGG
                     MRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKSEFNTAIIMITHDLG
                     VVAGICDNVLVMYVGRTMEYGTAEQIFYTPSHPYSIGLIGAVPRVDGQEEELITIPGN
                     PPNLLRLPSGCPFQPRCVHATKQCLKAPALTLLNDQRLRACFVPAEQLIRGNAS"
     gene            1663..2652
                     /gene="oppF"
                     /locus_tag="PXD81_000937"
     CDS             1663..2652
                     /gene="oppF"
                     /locus_tag="PXD81_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000994906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppF"
                     /protein_id="HDL5536982.1"
                     /translation="MNTEKKVILEVKDLSVHFNIKDTKSLFFAKHQTLKAVNHVSFQL
                     YEGETLGVVGESGCGKSTLARAIIGLVESSGGNILWLGKELTQQSAKEWKTIRSDIQM
                     IFQDPLASLNPRMTIGSIIAEPLKIYQPHLTAEEVKEKVRAMMMKVGLLPNLINRYPH
                     EFSGGQCQRIGIARALIVEPKLIICDEPVSALDVSIQAQVVNLLKSLQREMGLSLIFI
                     AHDLAVVKHISDRVLVMYLGNAVELGDYEQVYHDTKHPYTKALMSAVPIPDPQLERNK
                     QIQLLEGDLPSPINPPSGCVFRTRCPLADQHCIADKPLLKGRDNHVVACFKAE"
     gene            complement(2756..3514)
                     /locus_tag="PXD81_000938"
                     /pseudo
     CDS             complement(2756..3514)
                     /locus_tag="PXD81_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014326384.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
     gene            complement(3644..4285)
                     /gene="bioD"
                     /locus_tag="PXD81_000939"
     CDS             complement(3644..4285)
                     /gene="bioD"
                     /locus_tag="PXD81_000939"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005724996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HDL5536983.1"
                     /translation="MAKVFFITGIDTDIGKTIATGWYAKKLMQQGASVITQKMIQTGC
                     RGIAEDLLIHRKIQGIELTEEDKQGITCPYVFDYPCSPHLAAKLEPRTIERKKIETCT
                     ALLCEKYDYVLLEGAGGLCVPYNEEETTLDYLCQHQYPVILVTSGKLGSINHTLLSLQ
                     VLNSKRVSVHAVIYNLYPETDQVISQETQHFLRRYLEKYSPNTLFEIMDLISV"
     gene            complement(4303..5058)
                     /gene="bioC"
                     /locus_tag="PXD81_000940"
     CDS             complement(4303..5058)
                     /gene="bioC"
                     /locus_tag="PXD81_000940"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005755527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HDL5536984.1"
                     /translation="MDRIDKGRIKQRFSAAVSNYDQQARAQQQIHQRLLSLLSYIDRQ
                     AFQRALEIGCGTGGLTKQLMSQLQVEHWELNDLCDMREHLQSVLPQPFHFHCGDAEQL
                     PFPQQYDLIASASVVQWFEDKAGFIARCKVRLKKHGFLLFSTFTPENLTQIKHLTGVG
                     LTYPRLEDWQKWLVPEFEILALEHEEIVLHFDDPLAVLKHLKQTGVTATQQQRWTKGQ
                     LHAFCQQYQQHYRNAQHKVELTYTPMYVLARLT"
     gene            complement(5062..5709)
                     /locus_tag="PXD81_000941"
     CDS             complement(5062..5709)
                     /locus_tag="PXD81_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005758126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF452 family protein"
                     /protein_id="HDL5536985.1"
                     /translation="MKTQHISQQGDNLIVYFAGWGTPPSVVEHLILPENYDLCLCYDY
                     RDLHCDVDFSAYQHIRVVAWSMGVWVAERVMQGYPLLSATAINGTGLPCDDQYGIPEA
                     IFAGTLSGLNEDNRTKFERRICGSKTLLRDYQHYSMRPTLQEIHAELTALYTFLQQDH
                     RTDLISWSKAIVGSKDKIFPAQNQRAYWQDRCAITEIAGEHLLFPHFTHWETCWL"
     gene            complement(5706..6869)
                     /gene="bioF"
                     /locus_tag="PXD81_000942"
     CDS             complement(5706..6869)
                     /gene="bioF"
                     /locus_tag="PXD81_000942"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005719374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HDL5536986.1"
                     /translation="MIRYYQQQLADLKAQNQFRQLPQLIHRGRFIQREDNTMLNMSSN
                     DYLGLANNEALRQAFFTQYQDQLPALTSSSSRLLTGSFPIYDELESLMAQAFGRETAL
                     LFNSGYHANIGILPALADKKTLIVADKLVHASMIDGIRLAQCEFVRFRHHDYVHLEQI
                     LQKNDRTFERIIVVTESVFSMDGDCADLTQLVALKQRYPQVMLYVDEAHAIGVLGEKG
                     LGLAEQQGCINQIDILVGTFGKALGSMGAYVICDQVIRDYLVNKMRPLIFSTALPPFN
                     VAWTHFVFQQLPHLQAERAHLAQLSQHLRQAIVDIFQVPMPSESCIVPYILGDNELTV
                     RTAQRLQQQGYYCLPIRPPTVPKGTSRIRFSLTADMQVAEVEQFIACLRELAE"
     gene            complement(6866..8167)
                     /locus_tag="PXD81_000943"
     CDS             complement(6866..8167)
                     /locus_tag="PXD81_000943"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016533607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HDL5536987.1"
                     /translation="MNEQQLLECDRQHIWHPYAALNADFPLFAVERAEGVYIQLKNGQ
                     QLIDGMSSWWSAIHGYNHPRLNHAATQQLSQMSHVMFGGLTHQPAVELAQQLVNILPE
                     SLDKIFFADSGSVAVEVAMKMAVQYQHAKGETQRYKFATIRSGYHGDTWHAMSVCDPV
                     TGMHSLFAKGLPVQYFLPQPPVKFGQAWQPEAMQPLQDLLAQHGDEIAGLILEPVVQG
                     AGGMYFYSPEYLVQAKALCEPYGILLIFDEIATGFGRTGKLFACEHANVVPDVMCIGK
                     ALTGGYLTLSATITNQAVADVICSGEAKCFMHGPTFMANPLACAVAAESIKLLLASPW
                     QQRIQQIEQQLRQELRQAEALDCVAEMRVLGAIGVIEMKAPVNMASLPPRFVQNGVWV
                     RPFGKLVYLMPPYIISSDQLSQLIHGTIRTLQEEYAHKESL"
     gene            8309..9196
                     /gene="psd"
                     /locus_tag="PXD81_000944"
     CDS             8309..9196
                     /gene="psd"
                     /locus_tag="PXD81_000944"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005755522.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HDL5536988.1"
                     /translation="MNILEKKAQLSYWQRIKIAFQYVMPQLYLTRLAGWFAKQQWGAV
                     THFVIKLFAKKYHVDMSEAAKPNFSDYASFNEFFIRPLADNARPINQNPTALCLPADG
                     RISQLGHIEQDLLLQAKGHYFSLNDLLAGDEALAHHFKDGEFATTYLSPRDYHRVHMP
                     CDATLCKMIYVPGDLFSVNPFLAEHVPNLFARNERVICVFDTEFGKMVQILVGATITA
                     SMSTVWAGVINPPRPEKITTWTYEGESAVKLLKGQEMGAFQLGSTVINLFEKDRVQLA
                     SHLQVDSPVRMGEILAHQK"
     gene            9221..9775
                     /locus_tag="PXD81_000945"
     CDS             9221..9775
                     /locus_tag="PXD81_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011609455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HDL5536989.1"
                     /translation="MSQQFFDQVSLESQLEVSAEAVAKGIFDVLNQNPPALELNEVSH
                     ALQTLQQQAKFKEIEAVVAQFLAENAQKAGVNTTESGLQYEVLVAGEGQIPAREDKVR
                     VHYTGTLIDGTVFDSSVKRGQPAEFPVNGVIAGWIEALSMMPVGSKWRLTIPHNLAYG
                     ERGAGASIPPFSTLVFEVELLAIL"
     gene            complement(9835..10158)
                     /locus_tag="PXD81_000946"
     CDS             complement(9835..10158)
                     /locus_tag="PXD81_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005755520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5536990.1"
                     /translation="MNWKAVLIFSVLGSLVSCASYSEHAVQRIEAGKSFAVTGNTKRI
                     NTMACQDNDDWYLDGYRVGKSFREHQQKMLIQRTAYCEEQTGKAVPDKFRHSWNSGYQ
                     QGLKR"
     gene            complement(10182..11285)
                     /gene="proB"
                     /locus_tag="PXD81_000947"
     CDS             complement(10182..11285)
                     /gene="proB"
                     /locus_tag="PXD81_000947"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005719361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HDL5536991.1"
                     /translation="MKIDKTLVVKFGTSTLTQGSAKLNLPHMIDIVRQLAQLHQAGFR
                     LVIVTSGAIAAGRHYLNHPQLPPTIASKQLLAAVGQSQLIQTWEKLFAIYDIHIGQIL
                     LTRADIEDRERFLNARDTLRALLDNQIIPVINENDAVATSEIKVGDNDNLSALVAILV
                     QAEQLYLLTDQQGLFDRDPRKHPDAKLIADVDKITDHIRAIAGGSGTSLGTGGMSTKI
                     SAADVATRSGIETIIAPGNRPNVIVDLAYGQAIGTKFSVQADRLESRKQWLYAAPSAG
                     IITIDAGAENAMLIQHKSLLPAGIVNVEGRFSRGEVVKIRTQQGKDVALGMPRYNSDA
                     LHLIQGKHSQDIEQVLGYEYGAVAVHRDDMIVL"
     gene            11451..12439
                     /locus_tag="PXD81_000948"
                     /pseudo
     CDS             11451..12439
                     /locus_tag="PXD81_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252470.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
     gene            12514..13161
                     /locus_tag="PXD81_000949"
     CDS             12514..13161
                     /locus_tag="PXD81_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3560 domain-containing protein"
                     /protein_id="HDL5536992.1"
                     /translation="MTINTYAKFAPTVFVAKCSESHTKGEIITLTSKYGNETEVQVHN
                     LVKQDGEHYFYSFTRCDGMDSQSFAQQKAERYQGYADNAAKRSQDWVNAANEGCDFLS
                     LGEPIKIGHHSEKRHRNLIERNARRMDNAVAEMNKAASYESRIAYWEKMAGKIDLSMP
                     ESLEYFKFELDKAKTKHKELKDNPEKREHGYSLTYAKKAVNELEKKVKLAELLWA"
     gene            13185..13592
                     /locus_tag="PXD81_000950"
     CDS             13185..13592
                     /locus_tag="PXD81_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme utilization protein"
                     /protein_id="HDL5536993.1"
                     /translation="MNRQGIYINGKQVFDSQGEDIQSIGFFGCNIQTVVGINKGSILV
                     SSNSNQLLVNGQSIELGSEPVLKIEVLGNVQKLSAGAADVSITGDAGSVDTVSGDVSI
                     KGNVASYVKTVSGDVRCRAITGSVETVSGDIYR"
     gene            13690..14040
                     /locus_tag="PXD81_000951"
     CDS             13690..14040
                     /locus_tag="PXD81_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5536994.1"
                     /translation="MTTINLSQQERELVEFVWNNRNTPWFFHCSVSNVSRNNTSRQIA
                     VCVIKDNQLISLDELIAKLCGYKLRQNGLYVTVHNMDAVFDTLYRFMCKLNDEMEANE
                     NVCPYLSLAHYEVF"
     gene            14131..14493
                     /locus_tag="PXD81_000952"
     CDS             14131..14493
                     /locus_tag="PXD81_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5536995.1"
                     /translation="MIDTFFDLDDTSCYLGNWEDILKEYDDCLSVEDFWHSAKEFDTV
                     PHFGNLYQERVILKLIARFCIELNIEQDSGLVEFDYYINAIDTHFYINSQRICDIDDW
                     NEMLDKIREEMTPAKLVA"
     gene            14606..>14851
                     /locus_tag="PXD81_000953"
     CDS             14606..>14851
                     /locus_tag="PXD81_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5536996.1"
                     /translation="MTFELGRVCATRAVAECFNQHTLVDLLHRYCSGDWGNLCDNDKK
                     ANVKAIERGGRILARYDVEDETLYVITEADRSYTTILF"
BASE COUNT         4287 a         3211 c         3082 g         4271 t
ORIGIN      
        1 actcctgaga agcgacggct atccttagag gtagttgagt tattgccaaa atgtctccta
       61 gcattctgaa agggaacgcc acttttgtgg gaatgccacc taaaccctct accatctcag
      121 tgaagcatca ttgagatttc attttacttc cacctcgcgg ggaacatttt tcccgtgttg
      181 ggcaaggatg tttctccgct tttttacatt tggagaaaca aactatgaca actcaaactt
      241 ctactcaaaa aaattacttc aaccttcata caacaggcat tggctatcta aacgatattc
      301 gtttggtaga gcctaaaaaa ggaaatcctt tttatgcctg ccgtatagca gctctggttg
      361 gcgaatctga ctctcctgag tacagatatt ttgatatgaa tgtagtcggt aaagagactg
      421 aaacacttat caaaagatgc caaaaagcag ttagtgaaga gaaaaaagtg cttgtgtcat
      481 ttgtaatggc tgatttgtgg ttcgatacat ttacttactc aacagatagt aaattccaca
      541 aaaaaggcga taccggtgta tctgtaaaag gtcgcttaat ccgcatcaaa atgattaaaa
      601 ttgatgggga aatagcctac caagaaaagc caaaatcaga tgagcaatct gagtaatttt
      661 acctaagccc gacattttat gttgggcttt attactcgat gtacaagatt tgcgcgtaac
      721 ttttaagacc ccagatggcg atgttactgc cgtcaatgat ctgacttttt ctttaaatgc
      781 cggtgaaacc ctcggcattg tcggcgaatc tggctcagga aaatcgcaaa ccgcttttgc
      841 tctaatgggg ctattagcag ataacgggcg aatttcagga tcagcaaaat ttaatggaca
      901 agaaattctc aacttgcctg aaacacaact taaccgcttg cgtgctgaac aaattgcgat
      961 gatcttccaa gatccaatga cctcgctgaa cccttatatg aaaatcggcg aacagctaat
     1021 ggaagtactg atgttgcata aaggcatgga tcataaaact gcgtttgaag aatcggtacg
     1081 gatgttagat gcggtgaaaa tgccggagtc acgtaaacgt atgaatatgt atccgcatga
     1141 attttccggc ggtatgcgtc agcgtgtgat gattgcgatg gcattacttt gccgtccgaa
     1201 actcctcatt gcggatgaac ccacaactgc gctcgatgtg acggtgcaag ctcaaatcat
     1261 gaccttatta aacgaattaa aaagcgagtt taataccgcc attatcatga ttactcacga
     1321 tctcggtgtg gtcgcaggaa tttgcgataa tgtgttggtg atgtatgtag gcagaaccat
     1381 ggaatacggc accgctgagc aaattttcta taccccaagc cacccttatt ctatcggctt
     1441 aattggtgca gtgcctcgcg ttgacggaca ggaagaagaa ctcattacta ttccgggtaa
     1501 tccacctaac ttattacgct taccgagtgg ctgtcctttc caaccacgtt gcgttcatgc
     1561 aactaaacag tgcctcaaag caccggcgtt aaccttgtta aatgatcaac gtttacgcgc
     1621 ttgttttgtc cctgccgagc aattaatccg aggtaacgca tcatgaacac agagaaaaaa
     1681 gtgatcctag aagtgaagga tttaagcgtt catttcaaca tcaaagatac gaaatcttta
     1741 ttctttgcta aacaccaaac tttaaaggcc gtcaatcatg tctcttttca gttatacgaa
     1801 ggcgaaacct taggtgtggt cggtgaatcg ggctgtggaa aatcaactct cgcacgcgcg
     1861 attattggct tagtggaaag ctctggcggt aatattttat ggctaggtaa ggagttaact
     1921 caacaaagtg cgaaagaatg gaaaacgatc cgtagcgata ttcaaatgat tttccaagat
     1981 cctttagcct ccctgaatcc acggatgacc attggcagta tcattgccga accattaaaa
     2041 atttaccaac cccatttaac cgcggaagaa gtgaaagaaa aagtacgcgc gatgatgatg
     2101 aaagtgggct tattacctaa cttaatcaat cgctatccac atgaattttc gggtggacaa
     2161 tgtcagcgta tcgggattgc gcgcgccttg attgtggaac caaaactcat tatttgcgat
     2221 gagccagtgt ccgccttaga tgtttcaatc caagcacaag tagtgaattt attgaaatct
     2281 ttacaacgcg aaatggggct atcattgatt ttcattgcac acgaccttgc tgtggtgaaa
     2341 cacatttccg atcgtgtttt ggtcatgtat cttggtaacg cggtggaatt aggtgactat
     2401 gaacaagtct atcacgatac taagcatcct tataccaaag ctttgatgtc tgcggtaccg
     2461 attcctgatc cgcaattaga acgcaataaa caaattcaat tgcttgaagg cgatctccct
     2521 tccccgatta atccgccgtc gggctgtgtg ttccgcaccc gttgcccact ggcagatcaa
     2581 cactgtattg cagacaaacc gctcctcaaa ggacgagata atcacgttgt tgcttgtttt
     2641 aaagccgagt aatacacaca accgcacaaa aaagtgcggt tgtttttttg cgaatattca
     2701 cagtcagact ttcgtgatac tttcgctctg agataaggat tacgatacag agaagttatt
     2761 caacaatcac acgtgcattg atcggttgca ctggacgatt attgtgtcct ttcatcaagg
     2821 tcgcaaacgc ctcaacttga gattggctaa tgttgtcata gtgttttaac actaaccaat
     2881 ttacaccttc agaacaaggt ggtgtagtta atgacccgct gaaacgatag taatcgcgtt
     2941 ttttcggaat taatgttgct gggtcaaaag cggtactaag cgtatatttc tcccctgctt
     3001 tttgcgggaa gttttgccaa attggggcta accattggtt ttcacttgat aatacataga
     3061 gcacgccaag cacagcaaga ttcccttcat tgtctgcgtg tacaaaatgt gcttccatcg
     3121 ggaaggattt acctcaaatt aagttttcac tcggcacatg gaaatggaat tgtttaaggg
     3181 taaaagtctt tccattaagc tgcatgcgat tacttgcctc tttgaaatcc acttgaatag
     3241 tatgcccatt attgacaatc tccacattgg aaggtcgata atccagctta atcggcgcta
     3301 acttaccatc aatcgtatgc ttgatatcta ttggtgtttg gtttttacct tcgccacaaa
     3361 tacggaattt gggtgaaagt tcagcccaat gttctggcga ttcgtgtccg gtgtatcccc
     3421 aatgcgtgtg cgtttcttct ttcttaaggg ggcttgtaca cgctgtcaat gctaatgcac
     3481 ctaacgtaag cacaaaaagg tttgattttt tcatagccta gctcctgatt aaaagagatg
     3541 aattgatgag acaacctaaa ctcaatgatg ccttaatccg agacgctttc atcttaggca
     3601 aaacaacact ccatttcaag tataacttgc tcaagaatat atcttagacc gaaatcaaat
     3661 ccattatttc aaataaggta ttaggcgagt atttctctaa ataacgacgt aaaaaatgtt
     3721 gtgtttcttg gctaatgact tgatctgttt ccggatacaa gttgtatatc acagcatgca
     3781 cacttactct tttggagttg agtacttgta aactcaataa ggtatgatta atactaccta
     3841 gcttgccaga ggtaactaaa atcacggggt attgatgctg acaaagataa tcaagggtgg
     3901 tttcttcctc attataaggt acacacaagc cgcccgcccc ttccaataat acataatcat
     3961 atttttcaca caaaagtgcg gtgcaggttt caattttttt acgttcgatc gtgcgcggtt
     4021 ctaatttcgc ggctaaatgc ggtgagcaag gataatcaaa cacgtaagga caagtgatac
     4081 cttgcttatc ttcttctgta agctcaatac cttgtatttt tcggtgtatg agtaaatctt
     4141 cggcaattcc tcggcagcct gtttggatca tcttttgcgt gatgacactt gcgccttgtt
     4201 gcatcagttt cttggcgtac caacctgtgg cgatggtttt gccaatatcg gtgtcgatgc
     4261 cagtgataaa aaaaaccttt gccatacttt ttccttctaa gattaagtta aacgcgccag
     4321 cacatacatc ggcgtgtaag tgagttctac tttgtgttgt gcgttacggt aatgttgttg
     4381 atattgttgg caaaaggcgt gcaattgccc ttttgtccac ctttgctgct gtgtggcagt
     4441 aacgcctgtt tgtttcaaat gttttaacac cgctaaggga tcatcaaagt gcaacacaat
     4501 ctcctcgtgt tcgagggcta aaatctcaaa ttcaggcact agccactttt gccagtcctc
     4561 aagacgtgga taagttaacc ccacaccggt gaggtgtttg atctgtgtta aattctcggg
     4621 cgtgaaagta ctgaataaca aaaatccgtg cttttttaag cgcactttac aacgcgcaat
     4681 aaaacccgct ttatcttcaa accattgcac cacggaagca ctggcaatca aatcatactg
     4741 ctgcggaaag ggaagttgtt ccgcatcgcc acaatggaag tgaaaaggtt ggggcaacac
     4801 tgattgcaga tgctctcgca tatcgcaaag gtcattcagc tcccagtgtt caacctgcaa
     4861 ctgtgacatt aattgcttcg ttaatccacc agtaccacat ccaatctcta atgcacgttg
     4921 gaatgcttgt ctgtcgatat acgacaataa actaagcaaa cgttggtgaa tctgctgttg
     4981 tgctctcgct tgctgatcat agttcgatac agcggcagaa aaacgctgtt taatgcgtcc
     5041 tttatcaata cgatccatgt gctacaacca acaagtttcc caatgggtga agtgaggaaa
     5101 caacaaatgc tcccctgcga tttctgttat tgcacaacga tcctgccaat aagcccgctg
     5161 attttgtgca gggaaaattt tatcttttga gccgacaatc gccttgctcc acgagattaa
     5221 atctgtacgg tgatcttgtt gtaaaaaggt ataaagtgcg gtcagttcag catgaatttc
     5281 ctgtaatgtg ggacgcatgg aatagtgctg ataatctctt aacagcgttt ttgaaccaca
     5341 aatacgacgt tcaaatttag tccgattatc ttcatttaaa ccagataacg tgccagcaaa
     5401 aatggcttcg ggaataccat attggtcatc acaaggtaag cccgtgccat tgatcgccgt
     5461 agcagatagc agtggataac cttgcatcac acgctccgct acccacacgc ccattgacca
     5521 tgccaccacg cgaatatgtt gataagcgga aaaatcaaca tcgcaatgca gatctcgata
     5581 atcatagcaa aggcataaat cataattttc cggcagaatc aggtgctcga ccacgctcgg
     5641 cggtgtgccc caccctgcaa aatagacgat cagattgtcg ccttgctgtg aaatatgttg
     5701 tgttttcatt cagcaagctc ccgtaaacaa gcaataaatt gttccacttc cgccacttgc
     5761 atatctgcag tcagtgaaaa acgaatacga gaggttcctt ttgggactgt cggcgggcga
     5821 atcggtaagc aataatagcc ttgttgttgc aaacgttgcg ccgttcttac tgtcagttcg
     5881 ttatcgccca aaatataagg tacgatacaa ctttcacttg gcatcgggac ttgaaaaatg
     5941 tcaacaatcg cttggcgtaa atgttgacta agctgagcaa gatgtgcgcg ttcggcttgc
     6001 aaatgtggta attgttgaaa gacaaaatgc gtccatgcca cattaaacgg cggaagtgcc
     6061 gtggaaaaaa tcaatgggcg cattttatta accaaataat ctcggatcac ttgatcgcaa
     6121 atcacataag cccccataga gcccaacgcc ttgccaaacg taccgactaa aatatcaatt
     6181 tggttgatac agccttgttg ttctgctaac cctagcccct tctcccccaa tacgcctatc
     6241 gcatgggctt catctacata taacatcact tgcggatagc gctgttttaa tgcgaccaac
     6301 tgggtgaggt ctgcacagtc accatccata ctaaacacgg attccgtgac cacgataatc
     6361 cgctcaaaag tgcgatcatt tttttgcaaa atttgttcaa ggtggacgta atcatgatga
     6421 cgaaaacgca caaactcaca ttgtgccaag cgaatgccat caatcatact ggcatgtacc
     6481 agtttatccg ccacaatcag cgtttttttg tccgctaaag caggcaaaat cccaatatta
     6541 gcatgataac cgctattaaa tagcaaggca gtttctcgtc caaatgcctg cgccattaag
     6601 ctttctagct catcataaat cggaaaagag cctgttaata aacgggagga agaagaagtc
     6661 aatgcaggga gttgatcttg gtattgcgtg aaaaatgcct gacgcaaggc ttcgttattg
     6721 gctaaaccta aataatcatt ggaggacata ttcagcatcg tattgtcctc acgttgaata
     6781 aaacgccctc ggtgaattag ttggggcagt tggcgaaatt gattctgcgc ctttaaatca
     6841 gctaactgct gctgataata cctgatcata gagattcctt atgtgcatat tcttcttgaa
     6901 gggtgcgaat tgtgccatga attaattgag acaattgatc cgatgagatg atatacggtg
     6961 gcatcaaata cactaatttg ccaaatggac gcacccaaac gccattttgc acaaagcgtg
     7021 gtggtaaact tgccatattg acaggcgcct tcatttcaat caccccaatc gctccaagta
     7081 ctctcatttc agcgacacaa tctaaggctt ccgcttggcg caattcttga cgtaattgct
     7141 gttcaatctg ttgaatacgt tgttgccaag ggcttgccaa taacaatttg atactttctg
     7201 ctgctaccgc acacgccagt ggatttgcca taaacgtcgg accgtgcata aaacattttg
     7261 cctcgccaga acaaatcacg tctgctaccg cttggttggt aatggtagcg gatagagtta
     7321 aatacccccc cgttaaagct ttaccgatac acatcacatc cggcacgaca ttggcatgtt
     7381 cacaagcaaa taatttgcct gtacgcccaa atcctgtggc aatttcatca aaaatcagca
     7441 aaatgccgta cggttcgcat aaggcttttg cttgcaccaa atactcaggg gaataaaaat
     7501 acataccacc cgccccttgc actaccggtt ctaaaattaa acccgcaatc tcatcaccat
     7561 gttgtgccaa taaatcctgt aacggttgca tcgcttcggg ttgccaagct tgcccaaatt
     7621 ttaccggggg ttgcggtaaa aaatattgca cgggtagccc tttggcaaac aagctgtgca
     7681 tgccggtcac gggatcacac acggacatcg catgccaagt atcaccatga tagcccgaac
     7741 gaatcgtagc aaatttatag cgctgtgttt cgcctttagc atgttgatat tgcactgcca
     7801 ttttcatcgc cacttccacg gcgaccgaac cactatcggc aaagaaaatt ttatccaaac
     7861 tttccggcaa gatgttgacc agttgctgcg ccagttctac cgctggttgg tgcgttaatc
     7921 caccaaacat cacatgactc atttgactca gttgttgcgt ggcagcgtgg tttaaacggg
     7981 ggtgattata gccatgaatc gctgaccacc acgatgacat gccatcaatc aactgctgac
     8041 cgttttttaa ctggatatac acaccttcgg cacgttctac tgcaaataag ggaaaatccg
     8101 catttaacgc cgcataaggg tgccaaatat gttgtcgatc acattctaaa agttgctgct
     8161 cattcatcgt ttcacctcgg ttgtgaatcg ccctttcaac tggcgtaaat tcacgcaaat
     8221 acttacataa acggaataac ttgttataat cgccaagcat ttggcttttc aagcccatta
     8281 ttctaactta aaaagatgat ttattactat gaatatatta gaaaaaaaag cacaactgag
     8341 ttactggcaa cggattaaaa ttgcctttca atatgtgatg ccacaacttt atttaacacg
     8401 tcttgcaggc tggtttgcaa aacagcaatg gggtgctgtc acacattttg tgattaaatt
     8461 attcgcgaaa aaataccatg tggatatgag tgaagctgca aaaccaaatt tcagcgacta
     8521 tgcgagcttt aatgaatttt ttatccgtcc attagcggac aatgcccgtc caattaatca
     8581 aaatcctacc gcactttgtt taccggcaga tggtcgtatt agtcagttag gacacattga
     8641 gcaggattta ttgctacaag ccaaaggaca ttatttcagc ttgaacgatt tgttagctgg
     8701 cgacgaggcg ctcgctcatc attttaaaga tggcgaattt gccaccacct acctctcacc
     8761 acgcgattac caccgtgtgc atatgccttg tgatgccacc ttatgcaaaa tgatttatgt
     8821 gccaggtgac ttattctcag tcaatccatt tttagccgaa cacgtgccaa acttatttgc
     8881 acgtaatgaa cgcgtgatct gcgtattcga taccgaattt ggtaaaatgg tccaaatttt
     8941 agtcggcgca accattaccg cgagtatgag caccgtgtgg gcaggtgtga tcaacccgcc
     9001 tcgtccagaa aaaatcacca cctggaccta tgaaggagaa agtgcggtca aattactgaa
     9061 aggacaagaa atgggagcct ttcaattggg ctcgacggtg atcaatttat ttgaaaaaga
     9121 tcgtgtgcaa ctggcatctc atttacaagt ggatagcccg gtacgtatgg gtgaaattct
     9181 tgcccaccaa aaataatttt taaccacaat aggaaaaaca atgagtcaac aatttttcga
     9241 ccaagtgtca ttagaaagcc aacttgaagt gagcgccgaa gccgtcgcga aagggatctt
     9301 tgatgtgtta aatcaaaatc caccagcact tgagttaaat gaagtgagtc atgccctaca
     9361 gactttgcaa caacaagcga aattcaaaga aattgaagcc gttgttgcac aattcttagc
     9421 tgaaaacgcg caaaaagcgg gggtaaatac tacggaatct ggtttacagt atgaagtgtt
     9481 agtggctggt gaagggcaaa ttccggcacg cgaagacaaa gtgcgtgtgc attacactgg
     9541 gacattaatc gacggtaccg tctttgacag ctcagtaaaa cgtggtcaac ctgcagaatt
     9601 cccagtaaat ggcgtgatcg ctggttggat tgaagccttg tccatgatgc cagtgggttc
     9661 aaaatggcgt ttaaccattc ctcacaacct cgcttatggc gaacgtggtg ctggtgcctc
     9721 cattccgcca ttcagtacct tagtgtttga agtcgaatta ttagccattc tttaaagcta
     9781 aacgctagac ataaaaaagt gcggtcattt tccgcacttt tttatgtcta gcgtttatcg
     9841 tttcaagcct tgctgatagc cgctgttcca cgaatggcgg aacttgtccg gcacagcctt
     9901 gcccgtttgt tcttcacagt aagccgttcg ttgaatcaac attttttgtt ggtgttctcg
     9961 aaatgatttt cccacacgat agccgtccaa ataccaatca tcattatctt gacatgccat
    10021 ggtattaatc cgttttgtat tccccgtcac tgcaaaagat ttgcctgcct caatacgttg
    10081 caccgcatgt tcagagtatg aagcacagct caccagcgaa cctagtacac tgaatatcaa
    10141 tactgctttc caattcatct tgttaccttt ctttttctga tttataacac aatcatatcg
    10201 tctcggtgta ccgcaaccgc cccatattca taaccgagaa cctgttcgat atcttgcgaa
    10261 tgtttgcctt gaattaaatg gagcgcatcg ctgttataac gtggcatccc taaggcaaca
    10321 tccttgcctt gctgagtacg aatttttacc acttcaccgc gtgaaaagcg cccttccacg
    10381 tttacaatcc cagcaggcaa taacgattta tgttgtatta acatcgcgtt ttccgccccc
    10441 gcatcaatgg taataatacc ggctgacggc gccgcatata gccattgttt acgactttct
    10501 aagcgatctg cttgcacact aaatttggtg ccaatggctt gcccataagc taagtccaca
    10561 atcacattcg ggcgattacc cggtgcaata atggtttcaa tgccggagcg ggttgccaca
    10621 tccgcggcac tgattttggt ggacatgcca cctgtgccta acgaggtgcc acttccgccc
    10681 gcaatcgcgc gaatatgatc ggtaattttg tccacgtcgg cgatcaattt ggcgtctgga
    10741 tgtttgcgcg gatcacgatc aaataaacct tgctgatcag taagcaaata caactgttct
    10801 gcttgcacca aaattgccac taacgcggat aaattatcgt tatcgcctac tttaatttct
    10861 gacgtagcga cagcgtcatt ttcattaatc actggaataa tctgattgtc taataaggcg
    10921 cgcaaagtat ctctggcatt taaaaaacgt tcacgatctt caatgtcggc acgggtcaat
    10981 aaaatctgcc caatatgaat atcataaatg gcaaacaatt tttcccacgt ttgaatgagc
    11041 tgactttgtc ctaccgcagc caataattgt ttcgaggcaa tcgtcggtgg caattgcggg
    11101 tgatttaaat aatgacgacc tgccgcaatc gcccctgagg taacaatcac taaacgaaag
    11161 cccgcttgat gaagctgtgc gagctgacgc acaatatcta tcatatgagg taaatttaac
    11221 ttcgccgatc cttgcgtaag cgtactggtt ccaaatttta caacgagtgt tttgtcgatt
    11281 ttcatcttct ctatcttctt atttttgtcg cacaaaattg aagctaactt accattaatc
    11341 atcagaaaaa tcatctcgtt tacttgctat tttctatgat ttagcacaaa ttttttctca
    11401 ctaaaaggca tataataaag cttcttgatt tttcagctag gaacccgatt atgacctcaa
    11461 cctcttcaca agaaacggag cggcaacgag aaatcactcg cctttgtgtt caagttgccc
    11521 aactcttata caacatggtg cagaaagtgc catcgttgtg caaatggcac atcgcttagg
    11581 cttagccctc ggtgtagata gcgtagaatg tgccctcacg ccaaatgcca ttattcttac
    11641 tacattagcc caagaccatt gcatcacgac agtccgtaaa aaccaagaca aaggaattaa
    11701 tatgcaagtg gttaccgaag tgcaacgtat tgtgattaca gcagaacgta aaatctatga
    11761 tatgcaacaa gtgcatgcca aactggctcg cattaaaccg cttaaataca atcgctattt
    11821 tgtgatgttg atgattggcg tatcctgtgc cagttttgct catttatctg gtggggacgc
    11881 actagtttca ttggttacat ttctcgccgc aacactcgcc atgtatgttc gccaatcact
    11941 tgccgcaaga cactataacc ccttaattgt atttgcggtg accgcctttg tcgcctcact
    12001 aattgcgggt atcgcgttga aatataacat cggcaatcag ccccaaattg ccttggcatc
    12061 cagcgtatta ttgctcgttc ccggctttcc actgattaat gcactcgccg atattttaaa
    12121 agggcatatc aatatgggga ttgcccgttg ggcactcgcg acaatcctca catttggggc
    12181 atgcttaggc attgtatttg cgctgagttt attcttagag gaaatctgta tgctaaataa
    12241 attcattcaa gatgaatttc tacggctaga tcaacgttat gcagcttggt atcaaagtga
    12301 agaaacggat gaagttgcca gaatggggat tcaagaaagt gagcggattt tgaaaattct
    12361 gtatgcactg aaatataacg aagaccctat gcaaaatggt ttaggctata ttgcatttat
    12421 ggcatcaaaa ggccaataat ttacttatct caacaattta cccaccgggg aaactcctcg
    12481 atggcgtttc ccctacttta taggagaaac gctatgacta ttaacactta tgcaaaattt
    12541 gcacctaccg tttttgttgc aaaatgctct gaatcacata ccaaaggtga aattattacg
    12601 ctaaccagca aatatggtaa tgaaaccgag gttcaagtac ataatcttgt taaacaagat
    12661 ggcgagcatt atttttattc atttactcgt tgtgatggga tggatagtca gtcatttgct
    12721 caacaaaaag cagaacgcta tcaaggctat gcagataatg cagccaaacg tagtcaagat
    12781 tgggtaaatg ccgctaatga ggggtgtgat tttttgagtt taggtgagcc aataaaaatt
    12841 ggtcatcaca gcgaaaagag acaccgtaac cttattgaac gtaatgctcg ccgtatggac
    12901 aatgcggttg cagaaatgaa caaagctgca agttatgaat ctcgtatcgc atattgggaa
    12961 aaaatggcag gaaaaattga cctgtctatg ccggaaagtc tggaatactt taagtttgaa
    13021 ttggataaag ccaaaacaaa gcacaaagaa ttgaaggaca accccgagaa acgagagcat
    13081 ggttattcac tgacttatgc taaaaaagca gttaatgaac ttgagaaaaa agtaaaatta
    13141 gctgaattac tttgggcata attatttaca aaggagatga cgatatgaat cgacaaggta
    13201 tttatattaa tggcaaacag gtgtttgatt cacaaggcga agatattcaa agcattggat
    13261 tttttggctg caatattcaa acggtcgtag gcataaacaa aggaagtatt ttagtctcgt
    13321 ccaatagtaa tcaattattg gtgaatggac agtcgattga attaggttct gagccagtcc
    13381 tcaaaattga ggttttgggc aatgtgcaga aactgagtgc cggtgccgca gatgtctcta
    13441 ttacaggaga tgccggcagt gttgacacag ttagcggtga tgtctctatt aagggaaatg
    13501 ttgcctctta tgtgaaaacc gtgagtggtg atgttcgctg tcgtgctatt accggttcgg
    13561 tggaaaccgt ctccggtgat atttatcgct gatatttact catcttatta tttaccaccc
    13621 tattgggaag caattttccc aaggtgggca gattgtttcc catttttatt ttgaatttgg
    13681 agaaacatga tgaccactat taacctttca caacaagaac gtgagttagt tgaatttgtc
    13741 tggaataacc gtaatacacc ttggtttttt cactgtagtg tatcgaatgt gtcgagaaat
    13801 aatacctctc ggcaaattgc tgtatgtgtc attaaggata atcaattgat tagccttgat
    13861 gaattgatag caaaattatg tggttataaa ctcagacaaa acggattata cgttactgtt
    13921 cataatatgg atgctgtttt tgatacctta tatcgcttta tgtgcaagct aaatgatgag
    13981 atggaggcaa atgaaaatgt ttgcccttat ttatcattag ctcactatga agtgttttga
    14041 ttggtatcag aactatccac cctgtcggga aacaattttt ccgatagggc agattgtttc
    14101 ccacttttat tttgaatttg gagaaacata atgatagaca ccttttttga ccttgacgac
    14161 acctcatgct atttaggtaa ctgggaggat atactgaaag aatatgatga ttgtctttca
    14221 gttgaagatt tttggcattc cgctaaagaa tttgataccg tacctcattt tggcaaccta
    14281 taccaggagc gtgtcatcct taagttgatt gctcgattct gtattgagct gaatattgag
    14341 caagattcag gtttagtcga atttgattat tatatcaatg caatagatac gcatttctat
    14401 attaatagcc aaagaatctg tgatatagat gattggaatg aaatgttaga taagattcgt
    14461 gaagaaatga cgccggctaa attagttgcc taatttttgc aaatttttac taaaaatgac
    14521 gcttactgtc gtcttaattc acccacttgg ggaaatatct ccccaatggg atgacatttc
    14581 ccctctttct gtttaggaga aatgtatgac ttttgagctt ggtagagtat gtgcaactcg
    14641 tgcagttgct gaatgtttca atcaacatac attagttgat ctacttcatc gctactgcag
    14701 tggtgattgg ggaaatttgt gtgataacga caagaaagcc aatgtgaaag ctattgaacg
    14761 cggaggacgc attttagctc gctatgatgt ggaagatgaa acactttatg taatcacaga
    14821 ggctgatcgt agttacacta caattttatt t
//
LOCUS       DANLYI010000043        14834 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.043, whole genome shotgun sequence.
ACCESSION   DANLYI010000043 DANLYI010000000
VERSION     DANLYI010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 14834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14834
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.043"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            complement(6..3281)
                     /locus_tag="PXD81_000954"
                     /pseudo
     CDS             complement(6..3281)
                     /locus_tag="PXD81_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015431593.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
     gene            complement(3529..4248)
                     /locus_tag="PXD81_000955"
     CDS             complement(3529..4248)
                     /locus_tag="PXD81_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HDL5536997.1"
                     /translation="MTQLTELTPALQALRERVLKANLELPKYGLVTFTWGNVSEIDRE
                     TGLVAIKPSGVEYDAMTVEDIVLVDLQGNRVYGDKKPSSDTETHLELYRQFTDIGGVV
                     HTHSRNAVGWAQAGRDLLALGTTQGDYFYGAIPCTRKMTAEEIAGSYELETGKVIVET
                     FRTRGIEPKAVPGVLVHSHGPFTWGKDGHDAVHNSVVLEEVAYMNFISHQIRPQIGPM
                     QQELLDKHYLRKHGANAYYGQ"
     gene            complement(4245..5105)
                     /locus_tag="PXD81_000956"
     CDS             complement(4245..5105)
                     /locus_tag="PXD81_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HDL5536998.1"
                     /translation="MRKHKLGIYEKALPKNISWQDRLSIAKVCGFDFVEMSIDETDER
                     LARLDWSKKERAKLVKTIINTGVTIPSMCLSGHRRFPFGSCDEATRQKAYEIMEKAIQ
                     LAVDLGIRTIQLAGYDVYYEEQDEGTIQRFQQGLEWAVELAASNQVTIAVEIMDTQFM
                     SSISRWKKWDEIIKSPWFTVYPDLGNLSAWNDNVAEELKLGIDKISAIHLKDTYKVTE
                     TCKGQFRDVPFGDGCVDFAACFKTLAELNYRGAFLIEMWTEKAEEPITEIINARRWIE
                     QKMQEGGFTL"
     gene            complement(5160..5906)
                     /gene="ulaR"
                     /locus_tag="PXD81_000957"
     CDS             complement(5160..5906)
                     /gene="ulaR"
                     /locus_tag="PXD81_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HDL5536999.1"
                     /translation="MNESYRHKELLALLEERKALSTADIMQLLNISPATARRDISKLD
                     GQGKLKKVRNGAEAILAPTFLQHTKITNNAQEKRRIAEAAGRLCKDGESLILTCGSTM
                     EMLGEVLCGRKLQIITNYLPLANQLIAGCHDDVVIMGGQYNKHKKVMLSINTQNEMTY
                     AATTMFTSGKGLTSEGLFKTDMIIANAEQQMAKHAERYVVLLDSTKLGKKVGMLFKEF
                     DKIDLLITGKEADPTIIQALRNKGLDIILA"
     gene            complement(5990..7081)
                     /gene="ulaG"
                     /locus_tag="PXD81_000958"
     CDS             complement(5990..7081)
                     /gene="ulaG"
                     /locus_tag="PXD81_000958"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HDL5537000.1"
                     /translation="MSKIQEITRESWILSTFPEWGTWLNEEIEEEVVPEGNFAMWWLG
                     CVGVWIKTPAGANICMDLWCGRGKSTKKVKDMVRGHQMANMAGVRKLQPNLRAQPMVL
                     DPFAINEVDFILASHYHSDHIDVNVAAAICNNPKLDHVKFVGPWHCAELWKKWGVPEE
                     RIIVVKPGDVVKLKDVEIHALDSFDRTCLVTLPVEGAEERGGELAGLCPSDEEMGRKA
                     VNYVFKTPGGTIYHGADSHYSIQFAKHGKQFDIDVALNNYGENPVGIADKMTSVDLLR
                     MAECLRCKVIIPVHHDIWTNFMASTQEIIDLWRMRKDRLQYQFHPFIWEVGGKYVYPR
                     DKDLIEYHHPRGFDDCFEQEPNIQFKSML"
     gene            7464..9260
                     /locus_tag="PXD81_000959"
     CDS             7464..9260
                     /locus_tag="PXD81_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate-specific subunit IIBC"
                     /protein_id="HDL5537001.1"
                     /translation="MDFLTEILLFLKDQVLNKAPFLLGIVACLGYILLKKDTSTIIKG
                     TIKTIVGFMIVQAGSGFLVTNFKPVIDGLAKYHQLTGAVIDPYTSMQSTIQTMGENYA
                     WIGYAVIGALFLNILLVVFRRLTGIRTIMLTGHIMFQQAGLVAVFYMIIGASMWETII
                     YTSVLMALYWGISSNIMYKPTQAVTGGAGFSIGHQQQIASWIAVKLAPKLGDKDDTVD
                     KMKLPNWLNIFHDSISATALVMTIFFGIILLSFGLDNLKELAGKTNQFMYILEMGLKF
                     AVAIQIIVTGVRMFVAELSEAFKGIAERLIPNSVLAIDCAAIYAFSPNAMVFGFMWGA
                     IGQFVAVGGLLAVGSPVLIIPGFIPMFFSNATIGVFANQFGGWKAVMKICFVMGIIEV
                     LGSAWVIQMLASQGTTFNGWMGMADWALFFPPILQGIISIPGFFFVIVAAAMVYMFFA
                     SKQLRAEEAEAAARGITLEQLDGYGLDEVKAEPKAEAVVQQAVENAENSANPIRILAV
                     CGSGQGSSMMMKMKIKGYLDKRGIPNIMDSCAVTDYKGKLSEVDIIVSSKHLANEIEV
                     PEGKAVLGVQNMLNPNSFGDELVELINQHNAK"
     gene            9340..10401
                     /locus_tag="PXD81_000960"
     CDS             9340..10401
                     /locus_tag="PXD81_000960"
                     /inference="COORDINATES: protein motif:HMM:NF012376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDL5537002.1"
                     /translation="MKKILSTLFMLSAVSVAVAKEVNIKLLGTSDVHGRVVPWSYGAD
                     VEDKSGSFAQIATYVKEVRANHKNVLLFDIGDAIQDNQVEEFAKTLDKAKNNPVPKVL
                     NAMNYDIFILGNHEFNFGMPNLDAILKDINAKILTANFYHKDGKRYVEPTTIIERDGV
                     KLGIIGLTTPMSAIFEKDTGHLDAMKFTSPIEEAKAQIADLKAKGVDAIIVLAHMGIE
                     NENNIPDTGVADLVNAVDGIDVIIAGHMHKDVPSETIKNTLITEPHRYGTVVSEVDLA
                     FDVNDKGEVKLLSKTAKTVPVKKLASDPEIEKIYQPYHNELRALNNVKIGETAQDLIP
                     QGKITVYRLLSRKTPECPH"
     gene            10389..10853
                     /locus_tag="PXD81_000961"
     CDS             10389..10853
                     /locus_tag="PXD81_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase C-terminal domain-containing
                     protein"
                     /protein_id="HDL5537003.1"
                     /translation="MSSLIHDVQQHYSGADVVSFAFDYETVRLDKGDIKKKDIIYNYR
                     YAGGDVSMYELTGKQLKQYMEWSADYFDTIQAGDKDYRYNAVRGKSKYVTFDLFGGVS
                     YNIDLRNPSGSKIVDLKLANGSLITDDGKYKVGMNSYRFGQLTKKGGIWEGQ"
     gene            10884..11000
                     /locus_tag="PXD81_000962"
     CDS             10884..11000
                     /locus_tag="PXD81_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ser/Thr protein phosphatase"
                     /protein_id="HDL5537004.1"
                     /translation="MGAEKGTIQNMLIDYITNVKGGKIEGKSHNRWAIIGLN"
     gene            11016..11483
                     /locus_tag="PXD81_000963"
     CDS             11016..11483
                     /locus_tag="PXD81_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDL5537005.1"
                     /translation="MNLKQSLIENNSILLNQSAANWEDAIKIGTDLLVKAGTIEPRYY
                     DNIVSKIKEMGPYIILAPGLAMPHARPEEGVIKTSFALVTFDTPIYFEGEDEPVHVML
                     TLAGSDSDQHMQGLVEITQVLDDPESDTGVDLDKIRRCKTAEEVYAVIDQALA"
     gene            11538..12218
                     /locus_tag="PXD81_000964"
     CDS             11538..12218
                     /locus_tag="PXD81_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HDL5537006.1"
                     /translation="MSKPLLQIALDSLSLEKAVADAKQAENQVEIIECGTILAFAEGM
                     KAVSTLRVLHPNHIIVCDLKTTDGGAILAKMAFEAGADWLTVSAAAHPATKAACKKVA
                     DEFNAAHPELKVKKEIQIEIYGNWTVEKDAKEWVELGVTQAIYHRSRDAELAGKGWTT
                     EDVELMKQLSDLGIELSITGGIVPEDIHLFKEIKNAKAFIAGRALVGEKGKQTAEAIR
                     VEIDKYWN"
     gene            complement(12286..13428)
                     /locus_tag="PXD81_000965"
     CDS             complement(12286..13428)
                     /locus_tag="PXD81_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HDL5537007.1"
                     /translation="MKIVIAPDSFKESLTALEVAQAIQQGFARVFPEAEYQLVPMADG
                     GEGSVQSLVDATHGSLQKISVIAPLANEVEGFFGLSGDQKTAFIEMAAASGLHLVPME
                     QRNPLVTTSYGTGQLIKAALDLGVEKIILGIGGSATNDGGVGMLQALGAKFLNAEQRE
                     IGFGGEALSQIQQIDLSGLDSRLKQVEFEVACDVDNPLCGERGASAIFGPQKGATPEM
                     VKMLDHALLHFAETVKSQLGIEIAEKAGAGAAGGMGGGLLLLPNVTLRSGVQIILEAT
                     DLAEKLADADLVVTGEGRMDAQSIAGKTPIGVAKVAKQFGKPVIAIVGSLKEDYPVVY
                     QHGIDAVFPIIRQVSNLEAVLKSGKENLISTAENVARLWKISALQE"
     gene            complement(13438..14817)
                     /locus_tag="PXD81_000966"
     CDS             complement(13438..14817)
                     /locus_tag="PXD81_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005757929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HDL5537008.1"
                     /translation="MSGIALILIFLVAIIIMIYMIAKWKVHPFLALMSISIALALVAG
                     LPLAKIPTIVGEGFSNTFKSIGIVIIFGAIIGTILEKTGAALKLADMVVKVVGQKRPE
                     LAMLIMGWVVGIPVFCDSGFVVLNPIREAIRKKILANPVGMAVALSAGLYASHVFIPP
                     TPGPIAAAGAVGLSHNLLLVIGMGFVVSIPVLIASYFFAKYVGKQVSVMEEAQADQVI
                     SQSYEDLIKQYGKLPSGFLSFAPIFMPIILMAIGSIAKIAGVSGDFGVFLQFVGNPII
                     ALAIGVIFAIFLLVSSGKMAEFDSLTNDTLKLVGPILFITAAGGVLGNVITQAGFVDY
                     IKQNAHIISTAGIFFPFLISAILKTAQGSSTVAIITTASIMGMFNAPDSLMNALGLTS
                     EMAAALTVMAIAAGSMCVSHANDSYYWVVTNFTKLTPQQGYRTQTTLTFIMGVVGMLT
                     VYLLSLILL"
BASE COUNT         4241 a         3378 c         2966 g         4249 t
ORIGIN      
        1 ttcagttaga aattaattct aatcccagca gtcacaacgt tgttgtgacc actatttttt
       61 gcgagtttaa ggtcgtaatt cacatataag gaagtatttt tatttacttc cgccaatgca
      121 ccaatcccta cccacgtttt atgttttgca agcccaatac cttttacgtc gaattttgca
      181 tcttgtaaac ctgtataact tgctttaaag ctaagatctt ctcgattaaa cgctttttga
      241 tgatttacat agccttggaa agtcgttttc atcccagcta aattcacctc taaagatgtt
      301 cgtaaaccaa tcaatccgct tgtttgttta taggttgctt tgtccgcggt gagaccaaat
      361 tgactgtttt gctcactgaa tgcgccacgt ctgacaacat catgactaaa accgacaaat
      421 ggagttaacg caaagcgacc ttgcttgaag tcatagcctg attcaacata agcagaaaat
      481 actctgtctc ggtggttgat ttttgctgta cttgcgttat tatccgccaa aataatatga
      541 cgctctacat ctgaatcaac actgccataa ccggctcgac cttgtaaata ccaaggtgtt
      601 ttatgcttat tgcctatacg ggcatataat gatgcaccta ttccatctgc ttttgaacta
      661 ccgccataac gatcgaagtt cacttttgct ttagaataat taagtgccgc acctaaaatc
      721 acatggtctg taactggctt atcaaaacct atttggccag cataggtatt ggttttacct
      781 tcaccaaacc ctatttcttt tagagtacca ttcccataaa tacccgttac tcacacacca
      841 gcagtctcac ctacattatc cagcgttcct aacattacaa gacggttaga gagatcttta
      901 ttcactgttt ctgagttttc aaaagtaagc gcttgcgcag acgcataaat ttgaccagat
      961 aagctatcta aaaccacact ttgcgtattg atgtttgcaa tactgttttg taaagtagcc
     1021 gcttgttgtg cgaattcatc atttgtagca tttgtagaac cattatttcc ttcaatttgg
     1081 ttatccaatg ccgaaaatga gctctctaaa gcagtcgcga catttttccg gctaactgtt
     1141 gcttttactt cattctcatt ggttttttcc gccgtcgcag ttaataaccc tgaggtttct
     1201 attgcagcaa agttaccctt aattgcggaa tcagaagtaa tagcactcat tgataaacct
     1261 ttttgggtca catactgagc tttaccctct tttaccgcag ctagtgataa agtagagccg
     1321 tttaagtcaa cttctccttt tacatttaag tttgaaccta aatctgacac tagcgtactt
     1381 tgtgctgaag caacgtaagc tctcttaatc gtagaataac cctggttgac aactgtaccc
     1441 tcgttattta cgccattttc taacgtggta ttattgacaa ccaatgtacc acttggagat
     1501 acgtttactt tagacacata ttttttaccg ttaatagtga cagcaccttc attgagtgtt
     1561 gtatcccctt caaaagtact agcacccgat aaaattaatt cgccttggcc atttttcacc
     1621 aagcctgcat caccgttaat attattggaa aactcatagc ttccatttgg aatatttaca
     1681 gtcacgttat cggctaaagc gagtcgttta ccaaaacgcg cagggccatt agtggctttt
     1741 tcaatattaa gtaaacccca tccatataca tcatcaactc caggagcgcc gatatcggtt
     1801 gccgtcgaga gaatagattg tttgataagg ttaccatcca tccaaggata tttttgtttc
     1861 acgagagctg ctgtacccgt aacggctgga gctgcaaagg atgaaccact agcaataatg
     1921 tttctacctc ctaaattaaa gatgtagtca cccattgcgg taacggtcca attttttgcc
     1981 acaccggctt gagaatatgg caataaattg ctccattcaa agttacccaa tccctgatta
     2041 gttctggttg ctaccgcaac aacagaaatc caaccttttt ccaagtcagc ttcataatga
     2101 ggtaacccag cttggagtga tggatcaaga tcaccacggt tattccccgc agcccagaca
     2161 aataagcccc cctcttttac tttatcctta tagaattgca acacattacc atcaaattgg
     2221 tgtaaatagt aatagctaga atttctattg ttattaaaat aagtaagagg ctttggaata
     2281 ccgtatgatt ggttaaaaat acgggctccc ctattccata agtcataata aattggagct
     2341 gttgcggata atcctgtacc cgcatttgtt gttttggtaa tatccgcaag taataacgtt
     2401 gacttagacg ctactccatt gcgagagttc cctcccccaa ccatttctgc gacctcaata
     2461 ccatgtgttg cagaagccgg ggcagtacgc cctgtttcaa taataacagc gcgattacca
     2521 aattttttga gtatatcgtc ctgcattaaa tagtttttaa aaccactatc taagataccg
     2581 actttaacac cttcaccagt taacttaaca ttttgtttgt tattaggatc atcaatgttg
     2641 taaccacgag agcggtcatt ccatcgaata cctacattgg gtacaccaac agctacaggt
     2701 tcaggatgaa gatttaccgg tgttgtaggc ctaatgataa ctggacgggt gtcatcaatt
     2761 ggcgattcaa tgacaggttt gttatctgta attggattag gctgtgtatc agtgatgggt
     2821 ttattatcaa ctattggttg gactggcgga tccataattg ggttggcatc aataaccggt
     2881 tgagttggcg tatctacaac gggtttattg gcaatcggtg tcggtttatt tacaattggt
     2941 ttgttcacag caggagcata agtggtacga acggttgttc tcccattatg cgttgtataa
     3001 acttttgtaa ctgtaatgcc attaactgtt gtcgaaacag ttctggttgt tacacctgca
     3061 ttgctacctg gataatacca cgacgaataa ccattacggc tattcgatgt atagtagccc
     3121 gaataaatcg gtgcatattt atagtaacta taataactat ccgtggcgga aggataatat
     3181 cttgttctac ttgcaagctg atctgaaagt tcagttgcgt taacctctgg cgataaggct
     3241 aatattgttg ctattgcaac aaaataattt ctatttttca ccttttggtt tccttctaat
     3301 aaatacaaag atactcttgt ttatttcaaa tctactacgg atccttaata gacttgtaaa
     3361 aacaagaggc agagattaga acaaaaatca aaattaaaat caagaaaagg aaaatggtaa
     3421 ttatttgtgg atctatccat aaactcaaac agactgagga gctattccga ttatagaaag
     3481 cccctcgaaa gctgttgata atttggaaaa taatttttag gttattagct attgcccata
     3541 ataagcattt gcaccatgct tacgcagata atgcttatct aataattcct gttgcattgg
     3601 gccaatttgt gggcgaattt gatggctaat aaaattcatg taagccacct cttctaatac
     3661 cacagaatta tgcacagcat cgtgtccatc ttttccccag gtaaatggac cgtgtgaatg
     3721 taccaacaca cctggaactg ctttcggttc aatacctcga gttcggaaag tttcgacaat
     3781 cacctttccg gtttccagct cataactgcc ggcaatttcc tctgcggtca ttttgcgagt
     3841 acaaggaatc gcaccgtaga aataatcgcc ttgagtggtg ccaagtgcca ataaatcacg
     3901 ccctgcttgt gcccaaccta cagcattgcg agagtgagtg tgtaccacac cgccaatatc
     3961 agtgaattgg cgataaagtt ctaagtgcgt ttctgtatca gaagacggtt ttttatctcc
     4021 gtaaactcga ttaccttgta aatcaaccaa tacaatgtct tccaccgtca tcgcatcgta
     4081 ttctacgccg gaaggtttga tggcgactaa accggtctca cggtcgattt cacttacgtt
     4141 accccaagtg aaagttacca agccatactt cggtaattct aaattggctt ttaacacacg
     4201 ctctcttaag gcttgtaatg caggggttaa ttcagttaat tgcgtcatag ggtaaatcct
     4261 ccttcttgca ttttttgttc gatccaacgg cgggcgttga tgatttcagt aatcggctct
     4321 tcggcttttt ccgtccacat ttcaattaag aatgcgccac ggtaattcag ctcggcaagg
     4381 gttttaaaac aagcggcgaa atctacgcaa ccgtcaccaa atggcacatc acggaactgc
     4441 cctttgcagg tttcagtcac tttgtaagtg tcttttaagt gaatggcaga gattttgtcg
     4501 atgccgagtt tcagctcttc cgctacgttg tcgttccacg ctgagaggtt gcctaaatct
     4561 ggatacacgg tgaaccacgg tgatttgatg atctcgtccc attttttcca gcgggaaatc
     4621 gaactcataa attgggtgtc cataatttcc accgcaatgg taacttggtt gcttgccgcc
     4681 agctccaccg cccattctaa gccttgttgg aagcgttgga ttgtgccttc gtcctgctct
     4741 tcgtaataga cgtcatagcc ggcaagctga atggtgcgaa taccgagatc gaccgccagt
     4801 tgaatggctt tttccataat ttcataggct ttttggcggg tcgcttcatc acaagaacca
     4861 aacgggaaac ggcgatgacc ggacaagcac attgacggaa tggtcacgcc ggtgttgatg
     4921 atggttttca ccagcttggc acgttctttt ttgctccaat caaggcgggc taagcgttcg
     4981 tccgtttcgt caatcgacat ttccacaaag tcaaaaccgc agactttggc gatagaaagg
     5041 cggtcttgcc acgaaatgtt tttcggcaag gccttttcgt aaatacccag tttatgtttt
     5101 ctcatagttt tcccttgaag atgataagta aaattttgat taaatttgac cgctacaggc
     5161 taagccaaga taatatccag gcctttgttt cttaatgctt ggataatagt cggatccgcc
     5221 tctttgccgg taatcaataa atcaatttta tcgaactctt taaacagcat accgactttt
     5281 ttgccgagct tggtgctatc caataacacc acataacgtt ctgcgtgctt tgccatttgt
     5341 tgctcggcat tggcaataat catatcggtt ttaaataatc cttcgctggt taagcctttg
     5401 ccactggtaa acattgtggt agcggcataa gtcatctcat tttgggtgtt aattgagagc
     5461 atcacttttt tatgtttatt atattgccca cccataatca ctacatcatc gtggcaacct
     5521 gcaatcaact ggtttgcaag cggtagataa ttggtaataa tttgcaattt tctaccacat
     5581 aatacttcac cgagcatttc cattgtggaa ccacacgtca gaataagact ttccccatct
     5641 ttacataaac gaccggcagc ttccgctatt ctacgtttct cctgcgcatt gttagttatt
     5701 tttgtgtgct gtaaaaatgt aggagccaga attgcttctg ctccgtttct aacctttttt
     5761 aatttacctt gaccatctaa tttactgata tcacgtcttg ctgttgccgg agaaatattt
     5821 aacagctgca taatatctgc tgttgacagt gctttccgct cttccaacag ggctaataac
     5881 tctttgtgtc tataactttc gttcactact ttatcccatt actaatcact actgctccat
     5941 aatgaacttg ctaaaacgcc actctccagt gagagtggcg actgctcacc tatagcatgg
     6001 atttaaattg aatatttggc tcttgctcga agcagtcatc aaagccgcga gggtgatgat
     6061 attcaattaa gtctttatca cgtgggtaaa catatttgcc gcccacttcc caaataaatg
     6121 ggtggaattg gtattgtaag cgatctttac gcatacgcca taaatcaata atttcttgcg
     6181 tacttgccat aaagtttgtc caaatatcat ggtgaaccgg aataatcact ttacagcgta
     6241 aacactccgc catacgtaat aaatcaactg aagtcatttt atccgcaata ccaaccgggt
     6301 tttcgccata gttattcagc gctacgtcaa tatcaaattg cttaccgtgt ttagcgaatt
     6361 ggattgagta gtgagaatct gcaccgtgat aaatcgtacc gcccggggtt ttgaacacat
     6421 agttcaccgc tttgcgtccc atttcttcat ctgatgggca taagcctgca agttctccgc
     6481 cacgttcttc tgcaccttct accggtaagg tcactaaaca ggtacggtca aatgaatcta
     6541 gggcgtgaat ttctacatct tttaatttca cgacatcgcc cggttttacc acaataatac
     6601 gttcttccgg cacgccccat tttttccata attctgcaca gtgccaaggg ccgacaaatt
     6661 taacgtggtc taatttcggg ttattgcaaa tagccgctgc cacgtttaca tcaatatggt
     6721 ctgagtgata gtgagaagcg agaatgaagt ccacttcgtt aatcgcaaac ggatctaaca
     6781 ccatcggttg agcccgcaaa ttaggctgga gcttacgcac ccctgccata tttgccattt
     6841 gatgaccacg caccatatct tttactttct tggtggattt accacgaccg caccataaat
     6901 ccatacagat attcgcacca gctggggttt taatccacac accgacacag cctagccacc
     6961 acattgcaaa attgccttcc ggtacaactt cttcttcaat ttcttcattc aaccaagtac
     7021 cccattcagg gaaagttgaa aggatccagc tttcacgagt gatttcttga attttgctca
     7081 taatagtctc ctgatttacg ctaaaataag aacaatgcaa tcaaaaccaa tcataaacaa
     7141 tcacaaaaat ttgtacatct tttttattca aaaatgtgat ctatgtctaa ttttttatga
     7201 tttactggta aaaatttatt ttaataactt gatttaaata ccatttattt agcatatatg
     7261 agctaattta aagaataatc tttaataatc acgaataaat atatttttct tcctccatga
     7321 cgagattata aaaataaaat gtgatctcgc tcacaaataa ttaaaaataa tcttttttaa
     7381 tctttttcta gcgtttatca ttaattccga caacttctct ggagtttttt agttctatta
     7441 atctctcaac aaagaggccc cctatggact ttttaaccga aattctcctt ttcttaaagg
     7501 atcaagtgct gaataaagca cccttcttac taggtatcgt agcttgctta ggttacattc
     7561 ttcttaaaaa agacacttct acgattatta aaggcacaat taaaacaatt gtcggtttta
     7621 tgattgtaca agccggttca ggtttcttag taaccaactt taagccggtt attgatggct
     7681 tagccaaata tcaccaactt accggtgcag taattgaccc ttacaccagt atgcaatcga
     7741 ccattcaaac catgggcgag aactacgctt ggattggtta tgccgtaatt ggggcgctgt
     7801 tcctcaatat tctattagtg gtgttccgcc gtttaaccgg tatccgtacc attatgctca
     7861 ccggtcacat tatgttccaa caagcaggct tggttgcggt gttctatatg atcatcggag
     7921 cgtcaatgtg ggagaccatt atctacacct cagtgttgat ggctctttat tggggtattt
     7981 cgtctaatat tatgtataaa ccaacccaag cggtaacagg cggtgcaggc ttctcaattg
     8041 gtcaccaaca acaaattgct tcttggattg cagtcaaact cgcaccaaaa cttggcgata
     8101 aagacgatac tgtagataaa atgaaattgc ctaactggct caatattttc cacgacagta
     8161 tttctgcaac cgcattagta atgaccatct tctttggaat catcttactt tcattcggtt
     8221 tagataacct caaagaactt gctggcaaaa ccaaccagtt tatgtacatt cttgaaatgg
     8281 gcttgaaatt tgcggtggca attcaaatca ttgtaaccgg cgtacgtatg tttgtagctg
     8341 agctttcaga agccttcaaa ggtattgctg aacgcttaat tccaaactca gtattagcga
     8401 ttgactgtgc cgctatctac gctttctcgc caaatgcgat ggtattcggc tttatgtggg
     8461 gggctatcgg tcaatttgtg gcagtaggcg gcttgcttgc agtaggctca ccggtgctta
     8521 ttattccggg ctttatcccg atgttcttct ctaacgcaac cattggtgtg tttgcaaacc
     8581 aatttggtgg ttggaaagca gtaatgaaaa tctgttttgt gatgggtatt attgaagtgc
     8641 ttggctcagc ttgggttatt caaatgcttg cttcacaagg cacaaccttt aacggttgga
     8701 tgggtatggc tgactgggca ttgttcttcc caccaatcct acaaggtatt atcagcattc
     8761 cgggcttctt ctttgtaatt gtggcagcag caatggttta tatgttcttc gcatctaaac
     8821 aacttcgtgc cgaagaagca gaagcggcag ctcgtggcat tacacttgaa caattagacg
     8881 gctacggctt agatgaagta aaagcagaac caaaagcgga ggcagtagta caacaagcgg
     8941 tcgaaaatgc cgaaaattct gcaaacccaa tccgcattct agcggtgtgt ggctctggtc
     9001 aaggctcatc aatgatgatg aaaatgaaga tcaaaggcta tctcgacaaa cgtggtattc
     9061 cgaatattat ggattcctgt gcggtaactg attacaaagg caaattaagc gaggttgata
     9121 ttattgtctc cagcaaacat ttagccaacg aaatcgaagt accggaaggc aaagcggtat
     9181 taggtgtaca aaatatgcta aatccaaact catttggtga tgaattagtt gagctaatca
     9241 atcagcataa tgcaaaataa gtaagtttag gggtgaataa tcacccctat ctatgctaaa
     9301 gattgtaaaa actcacttca tcaaacgagg aattatccaa tgaaaaaaat cttatccacc
     9361 ctgtttatgc tctccgcggt tagcgttgcc gttgccaaag aggtaaacat taaactactt
     9421 ggtacctcag acgttcacgg acgtgttgtg ccgtggagct acggggcaga cgtggaagac
     9481 aaatccggct cattcgccca aattgcaacc tatgtcaaag aggtgcgtgc caaccacaaa
     9541 aatgtgttgc tgtttgatat tggcgatgcg attcaagaca accaagtcga agagttcgct
     9601 aaaaccttag acaaagcgaa aaacaatccc gtgccgaaag tgctgaatgc aatgaattac
     9661 gacatcttca ttctcggcaa ccacgaattt aacttcggaa tgccgaattt agatgcgatt
     9721 ttaaaagaca tcaacgccaa aattctcact gccaacttct atcacaaaga cggcaaacgc
     9781 tacgttgagc caactaccat cattgaacgt gatggcgtaa aacttgggat tatcggctta
     9841 accaccccaa tgtcggctat ttttgaaaaa gacaccggtc atttagacgc aatgaagttc
     9901 acttcgccga ttgaagaggc gaaagcccaa attgccgatc tcaaagccaa aggcgtggac
     9961 gccattattg tgctggcaca tatggggatt gagaacgaaa acaacattcc cgacaccggc
    10021 gtggcggatt tagtgaatgc agtggacggt attgatgtaa ttatcgccgg ccatatgcat
    10081 aaagatgtgc cgagtgaaac tatcaaaaat accctgatta ccgaaccgca ccgctacggc
    10141 acagtggttt ccgaagttga tcttgccttt gatgtgaatg acaaaggcga agtgaaacta
    10201 ctttctaaaa ccgccaaaac cgtaccggtg aagaaactcg caagcgatcc ggaaatcgaa
    10261 aaaatttacc aaccgtatca caatgaactg cgtgccttaa acaacgtcaa aatcggtgaa
    10321 actgcacaag atttaatccc acaaggcaaa atcacggtgt atcgattgct ttctcgcaag
    10381 acaccggaat gtcctcattg atccacgatg tgcaacaaca ttacagcggt gcagacgtag
    10441 tgagttttgc atttgactac gaaaccgtgc gtttagacaa aggcgacatc aagaaaaaag
    10501 acatcatcta caactaccgc tatgccggtg gcgatgtgag catgtatgag ctaaccggca
    10561 aacagcttaa acagtatatg gaatggtcag cggattactt cgacaccatt caagccggtg
    10621 acaaagacta ccgctacaac gctgtgcggg gtaaatctaa atacgttacc ttcgacctat
    10681 tcggcggcgt aagctacaac atcgacctac gcaatccaag cggtagcaaa attgtcgatc
    10741 taaaactggc aaacggcagt ctaatcaccg atgacggcaa atacaaagtg ggaatgaact
    10801 cctaccgctt tggtcagctc accaaaaaag gcggcatctg ggaaggacag taaatccccg
    10861 tgatttggga atccaaagtc gccatggggg ctgaaaaagg cacaatccaa aatatgctga
    10921 ttgattacat caccaacgtc aaaggcggaa aaatcgaagg caaatcgcat aaccgctggg
    10981 cgattatcgg cttaaattaa cgaaagggaa acactatgaa cttaaaacaa tctcttattg
    11041 aaaataactc tattttgctc aatcaatcag cggcgaattg ggaagacgca atcaaaatcg
    11101 gcactgattt attagtcaaa gccggtacga ttgaaccacg ctattacgat aatattgtaa
    11161 gcaaaatcaa agagatgggg ccttatatta ttcttgcacc gggcttggca atgcctcacg
    11221 cccgtccgga agaaggtgtg attaaaacct ctttcgcttt agtgactttt gacaccccga
    11281 tttattttga gggcgaagat gagccggttc acgttatgct aacccttgca gggagtgact
    11341 ctgatcagca tatgcaaggc ttggtagaaa tcacccaagt gctagatgac ccagagagcg
    11401 acacaggtgt tgatttagat aaaatccgcc gctgtaaaac ggctgaagaa gtgtatgcag
    11461 taatcgatca ggcattagcc taatattcta caagcggttc aaaaacatca atattttgca
    11521 aatagaggaa tacaattatg tccaaaccat tattacaaat tgcccttgat tctctttcac
    11581 tggaaaaagc ggtggcggat gcaaaacaag cagaaaacca agtcgaaatt attgaatgcg
    11641 gtactattct tgcttttgct gagggtatga aagccgtttc caccttgcgt gtattgcacc
    11701 caaatcacat tattgtgtgc gacttaaaaa ccaccgatgg cggtgcaatt ttagctaaaa
    11761 tggcatttga agccggtgcc gactggctca cggtttcggc tgcggctcac cctgcaacca
    11821 aagcggcttg caaaaaagtg gcggacgaat ttaacgctgc tcacccggag ctaaaagtga
    11881 aaaaagagat tcaaattgaa atctacggca actggacggt ggaaaaagat gccaaagagt
    11941 gggtagaatt aggggtaacc caagcgattt accaccgctc ccgtgatgcg gaactagcag
    12001 gtaaaggttg gacaacggaa gatgttgagt taatgaaaca gctttctgac ttaggcattg
    12061 aattatctat caccggcggt atcgtaccgg aagatattca cctgttcaaa gagatcaaaa
    12121 acgccaaagc ctttattgca ggccgtgctt tagtcggtga aaaaggcaaa caaactgccg
    12181 aagccattcg tgtggagatt gataaatact ggaactaatc cggcattgaa acccaataaa
    12241 aatggcgttc tctcaaccgg aacgccattt tttatctcat tttttctatt cttgcaaggc
    12301 cgatattttc cacaatctcg ctacattttc ggcagttgaa atcaggttct ctttgcctga
    12361 ttttaacacc gcttctaaat tgctgacctg tcgaataatc ggaaataccg catcaatgcc
    12421 gtgttggtac accacagggt aatcttcttt caatgagcca actatcgcaa tcaccggctt
    12481 gccaaactgc ttagcgactt tcgccacccc aatcggggtt ttaccggcga tagattgagc
    12541 gtccattcgc ccttcccctg tcaccaccaa atccgcatcg gcaagtttct cagcaagatc
    12601 ggttgcctca agaataattt gcacgccgga acgtaatgtt acgttaggca gtagcagtaa
    12661 accaccgccc attccaccgg ctgcaccggc accggctttc tcggcaatct caatgcctaa
    12721 ttgggatttt acggtttcgg caaaatgcaa cagggcgtgg tcgagcattt taaccatttc
    12781 cggtgtagct cctttctgtg gcccaaaaat tgccgaagcc cctcgctcgc cacacagcgg
    12841 attatcgaca tcgcaagcaa cctcaaattc cacttgcttt aagcgtgaat ccaaaccgct
    12901 taaatcaatc tgctgaattt ggcttaatgc ctcgccacca aagccgattt cccgctgttc
    12961 ggcatttaga aatttcgctc ctaaggcttg tagcattccg acaccgccat cgttagtcgc
    13021 actgccgcca atgccgagaa tgattttttc cacccctaaa tctaaggcgg ctttaattaa
    13081 ctgccccgtg ccgtagctgg tggtaaccag cggatttcgt tgctccattg gcactaaatg
    13141 caagccactt gccgccgcca tttcaataaa ggcggttttt tgatcccccg acaagccaaa
    13201 aaagccctcc acctcatttg caagcggtgc aattacgctg attttttgca aactgccgtg
    13261 agtggcatca accagcgact gtaccgagcc ttcgccaccg tctgccatcg gtacgagctg
    13321 atactccgcc tcagggaaaa cacgggcaaa accctgttga attgcttgag ccacttctaa
    13381 tgcggtcaaa ctctctttaa atgaatccgg tgcaatcaca attttcatcg tcaatcctta
    13441 gagtaaaatc aatgaaagca gataaaccgt gagcataccc accacaccca taatgaaagt
    13501 tagggtagtt tgcgtacgat agccttgttg cggagtcagt ttagtaaagt ttgttaccac
    13561 ccagtagtag ctgtcattgg cgtgggaaac gcacattgag ccagccgcaa tcgccattac
    13621 ggttaaggct gccgccattt cactggttag ccctaaggcg ttcattaatg agtccggtgc
    13681 gttgaacatt cccataatgg aggcggtggt gataattgcc accgttgagc tgccttgtgc
    13741 ggtttttaaa attgccgaaa tcaggaacgg gaagaaaatc cctgctgtac tgataatatg
    13801 agcgttttgc ttgatgtaat ccacaaagcc ggcttgagta atcacgttac ctagcacacc
    13861 accggcggcg gtaataaaga gaattgggcc gactaatttc agggtatcat tggttaagct
    13921 gtcaaattcc gccattttac cgcttgaaac cagtaggaaa atagcgaaga tcaccccaat
    13981 tgctaacgca ataatcgggt tgccgacaaa ttgtaggaat acgccgaaat cgccacttac
    14041 accggcaatt ttggcaatcg agccgatagc cattaagata atcggcataa aaatcggggc
    14101 aaagcttaaa aacccacttg gtaatttgcc gtattgtttg atcagatctt catagctttg
    14161 gctgattact tgatccgctt gagcttcttc catcacgctg acttgtttgc caacatattt
    14221 ggcaaagaag taactggcaa tcaacacagg aatcgaaacc acaaagccca ttccgatcac
    14281 aagcaggaga ttatggctta aaccaacagc accggctgct gcaatcgggc ccggtgtcgg
    14341 aggaataaag acgtgggagg catataaacc ggcacttaac gccaccgcca ttcccaccgg
    14401 attggcaaga atttttttgc gaatagcttc acgaatcggg tttaacacca caaagccgct
    14461 atcacagaac actgggatac caaccaccca gcccataatc aacattgcca gttcagggcg
    14521 tttttgcccc accactttta ccaccatatc ggctaatttg agggctgcac cggttttttc
    14581 taaaatcgtg ccgataatcg ccccaaaaat aatcacgata ccgatacttt tgaaggtatt
    14641 actaaaacct tcgcccacaa tagttggaat tttggcaagc ggtaaacctg ccactaatgc
    14701 aagggctatc gaaatcgaca ttaaggctaa gaacgggtga actttccact tcgcaatcat
    14761 ataaatcatt atgatgatag cgaccagaaa gatcagaata agtgcaatac ctgacataat
    14821 aagtctcctc attg
//
LOCUS       DANLYI010000044        14599 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.044, whole genome shotgun sequence.
ACCESSION   DANLYI010000044 DANLYI010000000
VERSION     DANLYI010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 14599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14599
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.044"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            56..328
                     /locus_tag="PXD81_000967"
     CDS             56..328
                     /locus_tag="PXD81_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005709555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HDL5537009.1"
                     /translation="MKKGIHPENYREVLFYDASVQQGWVIRSCAATNKTMVWEDGKEY
                     PLYTLDTSSASHPVYTGKRREVNTEGRASQFKDRFKGFASTLSNQK"
     gene            337..462
                     /gene="ykgO"
                     /locus_tag="PXD81_000968"
     CDS             337..462
                     /gene="ykgO"
                     /locus_tag="PXD81_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HDL5537010.1"
                     /translation="MKILNSLKTAKSRHPDCQIVRRKGKLYVICKTNPRFKARQR"
     gene            642..1415
                     /locus_tag="PXD81_000969"
     CDS             642..1415
                     /locus_tag="PXD81_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005602412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HDL5537011.1"
                     /translation="MLKKLSIAALALTLSVSAFAKESLLERINNKGTITVGTEGTYAP
                     YTYHDASGKLTGYDVEVTRAVAEKLDVKVEFKETQWDAMLAGLKGERFDLVANQVALT
                     SPERRATFDKSESYSWDGAALLVREDETKIKTPADVKGVKTAQTLSSNYGEIARENGA
                     DIVPIDGMAQGLLLIQQKRADATFNSHLSLLDYLKKNPNSGLKIVWEKEEKVGAGLIA
                     NKGNDEALAKISKAIVELREDGTLKKLGEQFFGKDISVQ"
     gene            1498..2214
                     /locus_tag="PXD81_000970"
     CDS             1498..2214
                     /locus_tag="PXD81_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HDL5537012.1"
                     /translation="MLNDLLLSIPFMTQARVDLVLSGFWPMVQAAFLVSIPLAIASFI
                     IGMLIAVAVALVRISPATGFFHRLLLWLVKGYISIIRGTPMLVQICIVFYGLPAIGIF
                     IDPIPAAIIGFSLNIGAYGSETIRAAISSVPKGQWEAGYTIGMTYMQTFRRIIAPQAF
                     RVAVPPLSNTFIGLFKDTSLASVVTVTEMFRVAQQVANMSYDFLPVYIEAGLIYWCFC
                     WVLFLIQARLEVRFNRFVAK"
     gene            2224..2976
                     /locus_tag="PXD81_000971"
     CDS             2224..2976
                     /locus_tag="PXD81_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HDL5537013.1"
                     /translation="MLKIRNIHKTFGNNAILRGIDLDIQKGQVVVILGPSGSGKTTFL
                     RCLNALEMPEQGTLEFSGNAPLKIDFAVKQSKKEILALRRKSGMVFQNYNLFPHKTAL
                     ENVMEGPVFVQQQAVEIAQKNAKALLEKVGLADKAELYPFQLSGGQQQRVGIARALAI
                     QPELMLFDEPTSALDPELVQDVLNTMKELANEGWTMVVVTHEIKFALDVADVVVVMDG
                     GEIVEQGSPQQLFNHPQHERTKRFLNQIRADK"
     gene            complement(3192..3725)
                     /locus_tag="PXD81_000972"
     CDS             complement(3192..3725)
                     /locus_tag="PXD81_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012263204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HDL5537014.1"
                     /translation="MEFLAPVISLIVGFLSFVLVLRTWLQFCRVDPRLPISQTLLKIT
                     EPMVSPVGKLIPTVKNICFPALLIAAALVTLQFILFGVDLPRAVLIGLLSVVKTFGQI
                     LFFTTLIRALMSWVTQGNHPLDYTVAQITEPVLGFIRKLLPRTGMLDFSVMVLGFGLL
                     LLNSLFYQIFGGLWAAA"
     gene            complement(3831..4172)
                     /locus_tag="PXD81_000973"
     CDS             complement(3831..4172)
                     /locus_tag="PXD81_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HDL5537015.1"
                     /translation="MAIQRNARQRKKMHLGEYQELGFLIKFTFAEGTGIDQIDETVDR
                     FIDEVIRPNGLALEANGYLNWEGLVCLEKLGKCDESHRQLVKTWLETNGLKEVQVSEL
                     FDIWWDYPAQA"
     gene            complement(4196..4945)
                     /gene="trmB"
                     /locus_tag="PXD81_000974"
     CDS             complement(4196..4945)
                     /gene="trmB"
                     /locus_tag="PXD81_000974"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005601989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HDL5537016.1"
                     /translation="MSEKQTFADKKRKTVEQAEFTEDGRYLRKVRSFVLRTGRLSDYQ
                     RDMMNNNWANLGLDYQPMPFNFEQIFGNNNPVVLEIGFGMGKSLVEMAEQNPNRNYIG
                     IEVHTPGVGACIAYALEKGVKNLRVICHDATEILRDSIADGTLGGLQLYFPDPWQKAK
                     HHKRRIVQPEFISRVIRKLSPNGFIHFATDWENYAEHMLEVLRQFESELQNTSATNDF
                     IPRPNFRPLTKFEERGHRLGHGVWDLYFTKK"
     gene            complement(4955..5905)
                     /locus_tag="PXD81_000975"
     CDS             complement(4955..5905)
                     /locus_tag="PXD81_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HDL5537017.1"
                     /translation="MNSRILATGSYLPTKVRTNADLEKMVDTSDEWIVTRSGIRERRI
                     AEPDETVATMGAKAAQQALEMAGIEANDIDLIVVGTTTNSHAYPSAACQIQGLLQIED
                     AIAFDVAAACTGFVYALTVADQFVRNGQVKKALVIGSDINSRMLDETDRSTVVLFGDG
                     AGAVILEASEELGIISTHLHASPDLENNLTLRSQKRGEERSGFIEMQGNATFKIAVNQ
                     LSSVVEETLEANNLQKSDLDWLIPHQANLRIITATAKKLEMEMDQVVVTLDKYANTSG
                     ATVPVALDEAVRDGRIQRGQLLLLEAFGGGWTWGSVLVKF"
     gene            complement(6051..7070)
                     /gene="hemB"
                     /locus_tag="PXD81_000976"
     CDS             complement(6051..7070)
                     /gene="hemB"
                     /locus_tag="PXD81_000976"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HDL5537018.1"
                     /translation="MQVIQSQYPLRRMRRLRKHDFSRRLVAENQLTVNDLIYPVFVIE
                     GENQRVQVPSMPGVERLTIDQLLVEAAELVKYGVPAVALFPVVGDVKKSLMAEEAYNP
                     QGLAQRAVRALKAQFPELGVITDVALDPFTTHGQDGIIDETGYVLNDVTTEVLVKQAL
                     SHAEAGADIVAPSDMMDGRIGKIREALEQNGFINTQIMAYSAKYASNYYGPFRDAVGS
                     AGNLKGGNKYTYQVDPANGNEGLHEVAMDIQEGADMVMVKPGMPYLDMVWRVKENFGV
                     PTFAYQVSGEYAMHMAAIQNGWLKERECIMEGLLCFKRAGADGILTYFAKRVAKWLYE
                     EAQAK"
     gene            complement(7151..7906)
                     /gene="tatC"
                     /locus_tag="PXD81_000977"
     CDS             complement(7151..7906)
                     /gene="tatC"
                     /locus_tag="PXD81_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HDL5537019.1"
                     /translation="MSQVDESQPLITHLIELRNRLLRSFICVLLVFCGLVYWANDIYT
                     LLATPLTDNLPDGATMIATNVATPFFTPIKLTIVVSVFLSVPFILYQIWAFVAPALYK
                     QEKRLVYPLLVSSTLLFYCGVAFAYYVVFPLVFGFLTSTAPEGVTMATDISSYLDFVL
                     TIFMAFGICFEVPVAIILLCWAGVTTPEDLKAKRPYIIVAAFVIGMLLTPPDIFSQTL
                     LAIPMCLLFEVGVLFARFYRPKDDDEPSEQVAQ"
     gene            complement(7893..8537)
                     /gene="tatB"
                     /locus_tag="PXD81_000978"
     CDS             complement(7893..8537)
                     /gene="tatB"
                     /locus_tag="PXD81_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatB"
                     /protein_id="HDL5537020.1"
                     /translation="MFDIGFSELVLIMIVGLVVLGPKRMPIAIRTVMGWVSTIRGLAA
                     NVQNELAQELKLQELKESIKKAEELNLSSLSPELNKTVEDLKMSATQLQDSLNSAKNE
                     LEKTAAESSTIPKLTDEQVAEIQQKIEQESQVLEAQELQKNAQNKPLEVDSLSEQLNV
                     TRTEKPLSADELAEQAEMDESLLTLESYYPPDDDLATPQSSESAQKSEANNVAS"
     gene            complement(8541..8762)
                     /gene="tatA"
                     /locus_tag="PXD81_000979"
     CDS             complement(8541..8762)
                     /gene="tatA"
                     /locus_tag="PXD81_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011199679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HDL5537021.1"
                     /translation="MGGISIWQLLIVVAIIVLLFGTKKLRTLGSDLGESVKGFKKAMA
                     DEKKDAEFEKVEAKDAISAEQKAKEKEQA"
     gene            complement(8873..10510)
                     /gene="ubiB"
                     /locus_tag="PXD81_000980"
     CDS             complement(8873..10510)
                     /gene="ubiB"
                     /locus_tag="PXD81_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HDL5537022.1"
                     /translation="MNIKNLNRFYHIIRTFLRYGIDEAIPNLPITRKIKLGRKALFWV
                     KNQHSDKPYGVRLRLALQELGPVWIKLGQMLATRRDLFAPELADQLALLQDNVEPFDG
                     KIARQIIEQALGGKLETWFENFDETALASASIAQVHTAKFNQNQPLAGKDVVLKVIRP
                     GIEPVIKADLELMYEIAHFIPKLSNDGKRLRAVEVVREYEKTILDELDLRKEMHNAIR
                     LRANFENSEMLYVPEMYQDFCHKNLIAMERIYGIPVSNIAELEANSTDMKLLAERGVQ
                     VFFTQVFRDSFFHADMHAGNIFVNREHPENPQYIGIDCGIVGTLNHNDKRYLAESFVA
                     FFNRDYRRVALMHVESGWTPPDTDIDKFEQAFREVCEPIFAKPLSEISFGHVLLNLFN
                     VAREFNMEVQPQLVLLQKTLLYIEGLGRQVYPQLDLWQTAKPFLQNWLNEQVGVKAMV
                     RDIKQRLPQYREHFAEFPEALFKALQEQKQINFRLAEINESLKAQNSNANSRFLVLSI
                     SAIVICGAFWKFESLPIWLSVPLLTLFPLVLILSQRK"
     gene            complement(10584..11561)
                     /locus_tag="PXD81_000981"
     CDS             complement(10584..11561)
                     /locus_tag="PXD81_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005825116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1016 family protein"
                     /protein_id="HDL5537023.1"
                     /translation="MKQIIAQARENAVRAVDFQRVLMYWHIGKRIFEQEQQGQERADY
                     GTYLIKMLAKELEPEFGSGFCSRQLERYRQFYRAFPIATALRSQLNWTQYRRLIQISD
                     SDKREFYLAESCKNNWSSRQLERQINSSLYERLLLSNDKESVLAVARNEQQPVDPKQL
                     IKDPMVLEFLGLKRESAYYEKDLEQAIITHLQDFLLELGNGFSFIARQKRLHLDGDDF
                     FVDLVCYNRLLQCFVIFEIKTHKLTHQDLGQLQMYVNYFDRVEKLPHENATIGVLLCA
                     DKNDAVVKFSLPENQNKIFASQYQLYLPSEQTLIAEVQKEIENFEQKYP"
     gene            complement(11722..12369)
                     /locus_tag="PXD81_000982"
     CDS             complement(11722..12369)
                     /locus_tag="PXD81_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HDL5537024.1"
                     /translation="MLNNVKQQLMLPQFTFGLIETAFNALLKRSPHIEPNLRKLSGKV
                     LKIALTSPKIDFFVLFSESRTDWLGNYEGEADCTVQLAFDTLPKLADKQKLTELINNK
                     SLVLTGDIQVLQHFTALLEQLEKDPAELLSPFVGDVVAQTSTDLAKKLLGKVKAQFEQ
                     NNQHLVENLIQERPVLVHRLQAVHFYDQVEELAQQALKLEQKFTHYVRSSSHQQS"
     gene            complement(12560..13312)
                     /gene="ubiE"
                     /locus_tag="PXD81_000983"
     CDS             complement(12560..13312)
                     /gene="ubiE"
                     /locus_tag="PXD81_000983"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015433517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HDL5537025.1"
                     /translation="MQQSAETTHFGFKTVAKEEKQQLVANVFHSVAGKYDLMNDLLSF
                     GIHRVWKRFTIDCSGVRKGQKVLDLAGGTGDFTAKFSRIVGESGEVILADINSSMLEV
                     GREKLRNLGVVGNVSYVQANAECLPFADNTFDCIVISFGLRNVTDKDKALRSMFRVLK
                     PGGRLLVLEFSKPIIDPISQLYNFYSFNILPKVGEVVVNDAESYRYLAESIRMHPKQD
                     ELKAMMEQTGFESVNYYNLSAGIVALHRGYKF"
     gene            13657..>14599
                     /gene="corA"
                     /locus_tag="PXD81_000984"
     CDS             13657..>14599
                     /gene="corA"
                     /locus_tag="PXD81_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011199502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HDL5537026.1"
                     /translation="MIRAFGLDNACLISVDETNPTLLNDAIWIDLIDPSEAERTVLEN
                     RLDQTLAEERELEDLEASARFFEDEDGLHLHSFFYCLDDDDYADIATVAFTIRDGRLF
                     TLRDRDLPAFRLYRMRSRREKLIDSNAYELLLDLFETKIEQLADVIEEVYADLEKLSR
                     VILNGQQENENFNDALSRLTEFEDASSKVRLCLMDTQRALSFLLRKTRLPNNQLEQAR
                     DIMRDIESLQPHNESLFQKVNFLMQAAMGYINIEQNRIMKFFSVVSVMFLPATLVAST
                     YGMNFEFMPELHFQYGYPMAIGLMIVAASAPYLYFKRK"
BASE COUNT         4162 a         3265 c         2854 g         4318 t
ORIGIN      
        1 atgtatccca tattttaatt atggttaatg accttatcta aattctggag aaaatatgaa
       61 aaaagggatt catcctgaaa attatcgtga agtgttgttc tatgatgcct cagtgcaaca
      121 aggctgggtt atccgttctt gtgcggcaac caacaaaaca atggtatggg aagatggcaa
      181 agaataccca ctttacacct tagatacctc atcggcgtct caccctgttt acacaggtaa
      241 acgccgtgaa gtgaatacag aaggtcgtgc aagtcaattt aaagatagat ttaaaggctt
      301 tgcttcaacg ctttcaaatc aaaaatagag gataaaatga aaatcttaaa ctcacttaaa
      361 accgcaaaat cacgccaccc ggattgccaa attgtcagac gcaaaggcaa actttatgtg
      421 atttgcaaaa ccaacccgag atttaaagct cgtcagcgtt aaaaaccagc accttaataa
      481 gctaatttat taaggtgttt tttatttcat cttctcaata ttacctattt taaccacatc
      541 aacgatttta ttatttctta ttccatttag ttatttgcaa gcggtcaatt ttccccttaa
      601 atttgcaaaa aatttatttt accaattaaa aggaaatgat tatgttgaaa aaattatcaa
      661 ttgctgcatt agctcttacc ctttctgttt ctgcatttgc taaagagagc ctacttgagc
      721 gtataaacaa caaagggact attactgtgg gtacggaagg aacttatgcc ccttacacct
      781 atcacgatgc gagcggtaaa ttaaccggtt acgatgtaga agtaacccgt gcggtggctg
      841 aaaaattaga cgtaaaagtg gaatttaaag agacacaatg ggacgctatg ttagccggtt
      901 taaaaggtga gcgttttgac ttagtggcaa accaagtggc tttaaccagc ccggaacgtc
      961 gagcgacatt tgataaatcc gaatcttaca gctgggacgg tgccgcctta ttagtgcgtg
     1021 aagatgaaac taaaatcaaa acccctgctg atgtaaaagg cgtgaaaact gcccaaacct
     1081 tgagctcaaa ctatggcgag attgcccgtg aaaacggtgc agacatcgtg ccaattgacg
     1141 gtatggctca aggcttactg ttaattcaac aaaaacgcgc cgatgcaact ttcaatagcc
     1201 acttatcgct attagattac ttgaaaaaga acccgaattc cggcttaaaa attgtgtggg
     1261 aaaaagaaga aaaagtgggt gcaggcttaa ttgcgaataa aggcaatgac gaagcattag
     1321 cgaaaatcag caaagcaatc gtagaattac gtgaagacgg cacgttaaaa aaattgggcg
     1381 agcagttctt cggtaaagat attagtgtac aataatccat attggattag gcttggggcg
     1441 aattcgcccc ttttattttt aaatctccct caaaacggga gattttaggt ttttcagatg
     1501 ttaaacgatc tacttctctc aatccccttt atgacccaag ctcgggtcga tttagtgcta
     1561 agcggttttt ggccgatggt acaagcggca tttttagtct ctattccgct ggcaatcgcc
     1621 tcatttatta tcggtatgtt aattgccgtt gcagttgcat tagtgcgaat tagccctgca
     1681 acaggctttt ttcaccgctt gctactgtgg ctggtaaaag gttatatctc gattattcgc
     1741 ggcacgccga tgttggttca gatctgtatt gtgttttatg gtttgcctgc aatcggcatt
     1801 tttatcgatc cgattccggc ggcaattatc gggttttcac tcaatatcgg ggcttatggc
     1861 tcggaaacga tccgtgctgc gatctcctcc gtgccaaaag gacaatggga agccggttat
     1921 actatcggta tgacttatat gcaaactttt cgccgtatca ttgccccgca agcgtttcga
     1981 gttgctgttc cgccacttag caacaccttt atcggtctgt ttaaagatac ctctcttgcc
     2041 tccgttgtta ccgtaaccga aatgttccgg gttgctcaac aggtagcaaa tatgtcttat
     2101 gacttcctac ctgtttatat tgaagccggt ttgatttatt ggtgcttctg ctgggtgcta
     2161 tttttaattc aggcgagatt ggaagtccgc tttaaccgat ttgtggcgaa ataaggagga
     2221 attgtgctta aaattcgcaa tattcataaa acctttggca acaacgctat tctacgcggc
     2281 attgatttag atattcaaaa agggcaagtg gtagtaattt taggcccttc cggttcaggc
     2341 aaaaccacct ttcttcgttg cttaaatgca ctggaaatgc ccgagcaagg cacattggaa
     2401 tttagtggta atgctccgct gaaaattgat tttgccgtaa aacagagcaa aaaagagatt
     2461 ttagcgctac gccgcaaatc cggtatggtg ttccaaaact acaacctgtt cccgcataaa
     2521 accgcattgg aaaatgtgat ggaaggaccg gtatttgtgc aacaacaagc ggtcgaaatt
     2581 gctcaaaaaa atgcaaaagc cctgttagaa aaagtcggac tggcggataa agcagaactc
     2641 tacccgtttc aactgtcagg cgggcaacag caacgggttg gcattgcacg agcattagca
     2701 attcagccgg agctgatgtt atttgatgaa ccaacttcgg cacttgatcc tgagttagtg
     2761 caagacgtgc taaatacgat gaaagaactg gcaaatgaag gctggacaat ggtcgtggtt
     2821 actcacgaaa tcaaatttgc ccttgatgtt gccgatgtag tggtggtaat ggacggcggt
     2881 gaaatcgttg aacaaggctc gccacagcaa ctgtttaacc acccacaaca cgaacgaact
     2941 aaacgttttt taaatcaaat cagagcggat aaataagaaa cgatagcgct agcgtccacg
     3001 ctagtgctat ctaataaatg gtacaagcgg ttaattatgt ataaattgtt atactgtttt
     3061 ggcacgagcg tggacgctcg cgccatcgta cagtaaaatc agctaacaag gttccccata
     3121 aaggataaat aagacaaggc tccttaatct tttagattaa ggagcttatt tttagatttt
     3181 agaaatccga tttaagccgc agcccatagt ccaccaaaaa tttggtagaa caagctattt
     3241 agtagcaata agccgaaacc tagcaccatt accgagaaat ctaacattcc cgttcttggc
     3301 aggagcttgc ggataaaacc taacaccggt tcggtaattt gtgctaccgt gtagtctaat
     3361 gggtggttgc cttgagtgac ccagctcatt aatgcacgaa tcaaggtggt gaaaaataga
     3421 atttgtccga aggttttgac cacacttaat aagccgatta atactgctct gggtaaatct
     3481 acgccaaaca gaataaactg taacgttacc aaagcggcgg caattaatag tgccggaaag
     3541 caaatatttt taacggttgg aatcagcttg ccaaccggtg aaaccatcgg ttcggtaatt
     3601 ttaagtaagg tttgtgaaat cggtaaacga ggatcaaccc gacaaaattg cagccaagtt
     3661 cttagcacca gcacaaaact taaaaagcca acaataagcg aaataacagg agctaaaaat
     3721 tccatctctt tttctctttc tatacaaaac aagcggtcgt tttttagaaa aaatttgcaa
     3781 attttcagta aaatctgacc gcttgtatct tattaaatta actaggcaaa ttaagcttgt
     3841 gctgggtagt cccaccaaat atcaaataat tcgctcactt gaacctcttt taagccgtta
     3901 gtttctaacc aagttttcac taattggcgg tgagattcat cacatttgcc taatttttct
     3961 aagcagacta aaccttccca gtttaagtag ccgttcgctt caagggctaa accgtttggt
     4021 cgaatcactt catcgataaa acgatctacg gtttcatcaa tttggtcaat gcctgtgcct
     4081 tcagcaaagg tgaatttaat taagaaacct aattcttggt attcacctaa gtgcattttt
     4141 ttacgttggc gtgcgttacg ttgaatagcc attttttctc tcctctatga ataaatcatt
     4201 tctttgtaaa atataaatcc cacaccccgt gtcctaaacg atgccctcgc tcttcaaatt
     4261 tggttagcgg gcggaaattc ggacgtggaa taaaatcatt agttgctgat gtattttgca
     4321 actcgctctc aaattggcgt aatacttcaa gcatatgttc tgcgtaattt tcccagtcgg
     4381 tggcaaaatg aataaagccg ttagggctta attttctgat cacacggctg ataaattcag
     4441 gctgcacaat acggcgtttg tggtgttttg ctttttgcca agggtctggg aagtaaagct
     4501 gtaagccacc taatgtaccg tcagcaatgc tgtctcgcaa aatttcggtg gcatcgtggc
     4561 aaattactct taaattcttc acgccttttt ctaacgcata agcgatacaa gcccccaccc
     4621 cgggcgtgtg cacctcaatg ccgatatagt tgcgattcgg gttttgctct gccatttcca
     4681 ccagcgattt tcccattccg aagccgattt ctaacactac cgggttgtta ttgccgaaaa
     4741 tctgctcaaa attaaagggc attggctggt aatctaagcc taaatttgcc cagttgttgt
     4801 tcatcatatc acgttgataa tcgctcaaac gaccggtacg caatacaaaa ctccgcactt
     4861 ttcgtaaata acgcccatct tcggtaaact cggcttgctc aacggtttta cgttttttat
     4921 ctgcaaaagt ctgtttttct gacattttct tgccttaaaa cttcactaat accgaacccc
     4981 aagtccagcc accgccgaat gcttcaagga gtagtaattg cccacgttga attcgcccgt
     5041 cacgcaccgc ttcatctaat gccaccggca ctgttgctcc actggtattg gcatatttat
     5101 caagggtaac caccacttga tccatttcca tctctaattt tttcgccgtt gcggtgataa
     5161 tgcgtaaatt cgcttggtgc ggaatcagcc aatctaaatc ggatttttgt agattattcg
     5221 cttctaaggt ttcttctacc acgcttgaaa gttggtttac tgcaatttta aaggtggcat
     5281 tgccctgcat ttcaataaag ccggaacgct cttcgccacg cttttggctt cttaaggtta
     5341 agttattttc taaatccggt gaggcgtgca agtgggttga aataataccc agctcttcac
     5401 tcgcctctaa aatcaccgcc ccagctccgt caccaaataa tactactgtg ctgcggtcgg
     5461 tttcgtctaa cattctggaa ttgatgtctg aaccaataac caatgccttt ttcacttgcc
     5521 cgttacgcac gaattgatcg gcaacagtta aagcatacac aaatcctgta caagccgctg
     5581 ccacatcaaa agcaatcgcg tcttcaatct gaagcaagcc ttgaatttgg caagctgcac
     5641 ttgggtaagc gtgggagttg gtagttgttc ccaccacaat taaatcaatg tcatttgcct
     5701 caattcctgc catttccagt gcttgttgag cggcttttgc ccccattgtt gccactgttt
     5761 cgtcaggctc ggcaatacgg cgttcacgaa tgccggaacg ggttacgatc cattcatcag
     5821 aggtatcgac cattttttct aaatcagcat tggtacgcac ctttgtcggt aaatagcttc
     5881 ccgttgctaa aattctacta ttcatatttt tattattctc aatcaattaa atgataatcc
     5941 gttattatag cttgaaaacc aacaaatata taggctttac aagcggtcat ttttagcaaa
     6001 aaagttgcat tcacgaacga ttttgcacta gaaccaccgc ttgctagtat ttatttcgct
     6061 tgagcctctt cgtatagcca cttcgctact cgcttggcga aataagttag aataccgtct
     6121 gctcctgccc gtttaaagca gagtaatccc tccataatgc actcacgctc tttcagccag
     6181 ccgttttgga ttgccgccat atgcatcgcg tattcgcccg aaacttggta ggcgaaggtt
     6241 ggcacgccaa aattctcttt cacacgccac accatatcca aataaggcat acccggtttc
     6301 accatcacca tatctgcccc ttcttgaata tccatcgcca cttcgtgcaa gccttcgttg
     6361 ccgttagccg gatcgacttg gtaagtgtat ttattaccgc cttttaagtt gccggcagaa
     6421 cctactgcat cacggaacgg tccgtaataa ttagacgcat atttagccga atacgccata
     6481 atttgcgtat tgataaaacc gttttgctca agggcttcac gaattttgcc gattcgaccg
     6541 tccatcatat cgctcggggc aacaatatcc gcccccgctt ctgcgtgcga taacgcctgt
     6601 tttaccaata cttcagtagt cacatcattc agtacatagc ctgtttcatc aataatgccg
     6661 tcttgtccgt gcgtggtaaa cgggtctaac gccacatcag taatcacgcc taattccgga
     6721 aattgagctt ttaaagctcg cacggcacgc tgtgccaacc cttgcgggtt ataggcctct
     6781 tctgccatca gtgatttttt cacatcaccc accacaggga aaagagccac cgcaggcacg
     6841 ccatatttaa ccagttctgc ggcttcaact aaaagctggt cgatagttaa acgctctaca
     6901 cccggcatag aaggcacttg tactcgctga ttttcccctt cgatgacaaa aacagggtaa
     6961 atcaaatcat taacggtaag ttgattttct gccactaagc ggcggctgaa atcgtgcttg
     7021 cgtaaacgac gcatacggcg taaaggatat tgggattgaa ttacttgcat ttttaatttc
     7081 ctaattctta aaatacctat tcttgaaata agaaaggcat agcaaacagg tgctatgcct
     7141 tttcaaaata ttactgtgca acctgctctg atggttcatc atcatctttg gggcgataaa
     7201 accgagcaaa tagcacacca acttcaaaca ataaacacat tggaattgcc aataatgttt
     7261 gcgagaaaat atctggtggg gtgagcaaca taccaattac aaatgctgcc acaataatat
     7321 aagggcgttt tgcctttaaa tcttcgggtg tggttacgcc tgcccaacat aacaagataa
     7381 tcgcaaccgg cacttcaaag cagatcccga atgccataaa aatggttaaa acgaaatcaa
     7441 gatagctact aatatccgtt gccattgtta ccccttccgg agcggtagag gttaaaaagc
     7501 caaatacaag cggaaataca acataatagg caaaagcgac accacaataa aacagcaatg
     7561 tgctagatac caataatggg taaaccaaac gcttttcctg cttgtataaa gcaggggcga
     7621 caaacgccca aatttggtac aaaataaaag gaactgataa aaacaccgaa accacaatgg
     7681 ttaatttaat tggggtaaaa aacggagtgg ctacattggt tgcaatcatt gttgcaccgt
     7741 ctggtaaatt gtccgttaaa ggtgttgcca ataaggtgta aatatcattg gcccagtaaa
     7801 ccaatccgca aaacaccaag agcacacaga taaaactacg cagtaatcgg tttctcagct
     7861 caattaaatg tgtaatcaga ggttgagact catcaacttg cgacattatt tgcctccgat
     7921 ttctgcgctg attctgatga ttgaggcgta gctaaatcat catctggcgg atagtaagat
     7981 tccaacgtta aaagcgattc gtccatttct gcttgttcag ccagctcatc agccgaaagt
     8041 ggcttttctg tccgcgtaac attaagttgc tcagataaac tatccacttc aagcggttta
     8101 ttttgcgcat ttttttgcaa ttcttgcgcc tctaacacct ggctttcttg ctcgattttt
     8161 tgctgaattt ctgcgacttg ctcatcggtt aatttaggaa ttgtgctgct ttccgcagcc
     8221 gttttttcca actcattttt tgcagaatta agcgaatctt gcaactgggt ggctgacatt
     8281 ttcagatctt ccaccgtttt atttaattct ggcgaaaggg agcttaaatt caactcttct
     8341 gcctttttaa tgctctcttt taattcttgc agtttcagct cttgagccag ttcattttgt
     8401 acgtttgcgg ctaaacctcg aattgtactc acccagccca ttacggtacg aatcgcaatc
     8461 ggcatacgtt tcgggcctag caccaccaag cccacaatca taatcagtac taattcagaa
     8521 aaacctatat caaacacgga ttaagcctgc tctttctctt ttgctttctg ctctgctgaa
     8581 attgcatctt ttgcttccac tttttcaaat tcagcatctt ttttctcatc tgccattgct
     8641 tttttgaagc cttttacaga ttcgcccaaa tccgaaccta aagtacgtaa tttcttcgta
     8701 ccgaaaagta atacgatgat tgcaaccaca atcagaagct gccaaatact aataccgccc
     8761 ataaaaactc ctttcattta atagggaata gataaaataa aacgctgttt attatatgtt
     8821 actttgacat ataaaaaagt aattttgcaa aaaatttgtc aaaaacaacc gcttatttgc
     8881 gttgactcaa aatcaatacc agtggaaata aagttaataa cggcacactt agccatatcg
     8941 gcaaagattc aaatttccaa aatgcgccac agatcacaat cgctgaaatt gacagcacaa
     9001 gaaaacggct atttgcgttt gaattctgtg cttttaaact ttcgttaatt tccgctaaac
     9061 ggaaattaat ttgcttttgc tcttgtaagg ctttaaacaa tgcctccgga aactccgcaa
     9121 aatgctcccg atattgcggt aaacgttgct taatgtctcg aaccatcgct ttcacaccta
     9181 cctgctcatt cagccagttt tgtaaaaacg gttttgcggt ttgccataaa tccaactgtg
     9241 gataaacctg cctgcccaaa ccttcaatat aaagaagtgt tttttgcaac aacaccaact
     9301 gcggctgcac ttccatatta aattctcttg ccacattaaa caggttgagc aaaacgtgtc
     9361 cgaatgaaat ttccgataac ggcttagcaa aaatcggctc acatacctca cgaaaggctt
     9421 gctcaaattt atcaatatcg gtatccggcg gcgtccagcc cgattccacg tgcattaagg
     9481 caacacggcg gtaatctcgg ttgaaaaatg cgacaaaact ttcggctaaa tagcgtttgt
     9541 cattgtggtt caacgtgccg acaatcccgc agtcaatgcc gatatattgc ggattttccg
     9601 ggtgttctct gttcacaaaa atattgccgg cgtgcatatc ggcgtggaaa aaactgtcac
     9661 ggaatacttg ggtaaaaaat acctgcacac cacgctctgc cagcaatttc atatcggtgc
     9721 tgttcgcttc caactcggca atgttagaca ctggaatgcc ataaatccgc tccatcgcaa
     9781 tcaagttttt atggcaaaaa tcttgataca tttcaggcac atacagcatt tcgctatttt
     9841 caaaattcgc ccgaagccga atggcattgt gcatttcttt gcgtaaatcc agctcgtcta
     9901 aaatagtttt ttcgtactct cgcaccactt caaccgcccg tagccgtttg ccgtcattcg
     9961 ataatttcgg gataaagtga gcaatttcat acatcaactc taaatccgcc ttaatcaccg
    10021 gctcaatgcc cgggcgaatc acttttaaca ccacatcttt gcctgcaagc ggttgatttt
    10081 ggttaaattt tgccgtgtga acctgtgcaa tcgaagcaga agctaaggca gtttcatcaa
    10141 agttttcaaa ccaagtttca agctttccgc ccaaagcctg ctcgataatt tgacgagcga
    10201 ttttgccgtc aaacggttca acattatctt gtagcaaggc gagctggtcg gcaagctccg
    10261 gtgcaaacag atcacgtctg gtagctaaca tttgcccgag cttgatccaa accggtccaa
    10321 gctcttgcaa agccaaacgt aaacgtacac cgtaaggttt gtctgaatgt tgattcttca
    10381 cccaaaacaa agccttacgc cctagcttta ttttgcgggt aattggcaga ttcggaatgg
    10441 cttcatcaat accatagcga agaaaagtgc ggataatatg gtaaaagcgg ttgagatttt
    10501 tgatgttcat tgaaatatat atttaatggt aattaggaag attttagcaa aattctgtaa
    10561 aaatttaacc gcttgttggg ctactatggg tatttctgct caaaattctc aatttctttc
    10621 tgtacttccg caataagtgt ttgttcagag gggaggtaaa gttggtattg gctggcaaaa
    10681 attttattct gattttccgg tagcgaaaat tttaccaccg catcattttt atcggcacaa
    10741 agcaagacac caatagtggc attttcgtgc ggcagttttt caactcggtc aaaataattg
    10801 acgtacatct gtaattgccc caaatcttga tgggtgagct tgtgggtttt aatttcaaaa
    10861 atcacaaaac attgaagtaa acggttgtaa caaaccaaat cgacaaaaaa atcatcaccg
    10921 tctaggtgca aacgcttttg gcgtgcgata aacgagaaac cgttacccaa ttctagtaga
    10981 aaatcctgca aatgcgtaat gatggcttgc tctaaatcct tttcgtaata agcactttcc
    11041 cgttttagtc cgagaaattc caataccatc gggtctttaa tgagctgttt cggatcaacc
    11101 ggctgctgct catttctggc aacagcaagc acactttcct tatcattact gagcaataat
    11161 cgctcgtata gactactatt aatttgccgt tccagctggc gtgaactcca attattctta
    11221 caactttcag ccaaataaaa ttcccttttg tcagaatcgg agatttgaat tagacgacga
    11281 tattgtgtcc aattcaattg cgaccgcagt gcggtcgcaa ttggaaacgc acgataaaac
    11341 tggcgataac gctctaattg acggctacaa aaaccacttc caaattctgg ctcaagctct
    11401 tttgcgagca ttttaatcag gtatgttcca taatcagcac gctcttgccc ttgttgctcc
    11461 tgttcaaaaa tccgttttcc gatgtgccaa tacatcagca cacgttggaa atctaccgca
    11521 cgaaccgcat tttcacgagc ttgagcaata atttgtttaa tatcttagat catcgttgtt
    11581 tctattaaca taaaagcctc cgttttggat taagtttatg cttaatctat ccggaggcaa
    11641 tttctttcac tcaacttttc gagtcatatc acaattttat tttgttaaat ttaattttgc
    11701 acaaacgctt gcgtttgaac atcagctttg ctgatggctc gaagagcgaa cgtagtgagt
    11761 aaatttttgc tctaatttga gcgcttgttg ggcaagttct tcgacttggt catagaaatg
    11821 caccgcctgt aaacgatgca ccaacaccgg tctttcttgg attaaattct caaccaaatg
    11881 ttgattattt tgctcaaatt gagcttttac tttacctagc aattttttag caagatcggt
    11941 gctagtttga gccactacat cgccaacaaa aggggaaagc agttcggcag ggtctttttc
    12001 gagctgttca agtaatgcag tgaaatgttg caacacttga atatcgcccg tcagcaccaa
    12061 cgatttatta ttaatcagct cggttaattt ttgcttatcc gctagtttcg gcagggtgtc
    12121 aaatgccagt tgcaccgtac aatctgcttc cccctcgtag ttgccaagcc aatcggttcg
    12181 gctttcgcta aacaacacaa aaaagtcgat tttaggcgaa gttagggcga tttttagcac
    12241 ttttccggac aatttacgca aattcggctc aatatgaggc gagcgtttaa gtaacgcatt
    12301 aaaagcggtt tcaatcagac caaaggtaaa ttgcgggagc attaattgtt gttttacatt
    12361 atttaacatt cgtacctact taccctcttc acttttggga gaaggataga ttgttcttca
    12421 ttaagaagca aaatcaggga gaggggtatt tgcaaaatat tgaataaata tgaccgcttg
    12481 ccccctctct ctgcttattt cgctaaacgc tcataagcgg tctctctccc acaaagggag
    12541 agagtcattt aagagaaaac taaaatttat aacctctgtg taatgccaca atgccggcac
    12601 ttaagttgta gtaattcacg ctttcaaagc cagtttgttc catcattgct tttagctcgt
    12661 cttgtttcgg gtgcattcgg atcgattcag ctaaatagcg gtaactttcg gcgtcattta
    12721 ccaccacttc gcccactttc ggcaaaatat tgaatgagta gaaattataa agctggctga
    12781 ttgggtcgat aatcggtttt gagaattcca acacaagtaa gcgaccgccc ggttttagca
    12841 cgcggaacat tgaacgtagg gctttgtctt tgtcggtaac attacgcaag ccaaaactaa
    12901 tcacaataca atcaaaagtg ttatcagcaa aaggcaagca ttcggcattc gcttgaacgt
    12961 agctcacatt tccaaccacg cctaaattgc gtaatttttc ccgcccgact tccaacattg
    13021 agctgttaat atcggctaaa atcacctcac cactttcacc aacaatgcga gagaattttg
    13081 cggtaaaatc acccgtgccg ccggcaagat ccagcacttt ttgcccttta cgcacgccac
    13141 tgcaatcaat ggtaaagcgt ttccacactc gatgaatgcc gaaagaaagc aaatcgttca
    13201 ttaggtcata tttgcctgcc accgagtgaa acacatttgc gaccagttgc tgtttttctt
    13261 ctttcgctac ggttttaaac ccaaagtggg tggtttctgc cgattgttgc acttcttgtt
    13321 gcacttcatc tttaggtgct aattgttcta catcgctcat tttcttgtct ctagttgcaa
    13381 aatttaggcg taaccatagc aaaaaagcgg tcgtttttct ttaacttttt gcaaattttc
    13441 ttaagtaaat aaccgctttc ttgactagaa aagccaaatt ctgtaacatt cgcacactat
    13501 tttttcttga ggattacaca ttttggacag tcatagtcga tacttacccg tttaacctat
    13561 cttgtctaac ctcaatgtta ccgacaaggt cggtaatgct cgcctagtat ctttgttcct
    13621 ttttgttcca agcctcctaa ctatttggag ctaaacatga tccgtgcttt tggactagat
    13681 aacgcatgtt taatcagcgt tgatgaaact aacccaactt tattaaatga tgcgatttgg
    13741 attgatctca tcgatccatc agaagctgaa cgaaccgtat tagaaaaccg cttagaccaa
    13801 acactcgctg aagaacgtga attggaagac ttagaggcgt ctgcccgttt ctttgaagat
    13861 gaagacggtt tgcacctgca ctcgttcttt tactgcttag atgatgacga ttacgccgat
    13921 attgccactg tcgccttcac tattcgtgac gggcgtttgt ttaccttgcg tgatcgggat
    13981 ttacccgcat ttcgcttgta tcgaatgcgt tcccgccgtg agaaattgat tgacagcaac
    14041 gcctatgaac tcttactcga tttatttgag accaaaatcg agcagcttgc agacgtaatt
    14101 gaagaagtct atgctgactt agaaaaatta agtcgggtta ttctaaacgg tcagcaagaa
    14161 aatgagaatt tcaatgatgc cctttctcgc ttaactgaat ttgaagatgc aagttcaaaa
    14221 gtgcgtttat gtttgatgga tacgcaacgg gcgttaagtt tcttactgcg taaaacccgt
    14281 ttgccgaata atcagctaga acaagcccgt gacattatgc gagatattga atctctgcaa
    14341 ccgcacaatg aatccttgtt ccaaaaagtg aattttttaa tgcaagcggc aatgggttat
    14401 atcaatattg agcagaaccg aattatgaaa ttcttctcgg tggtgtcagt gatgttccta
    14461 cctgctacgc tggttgcttc tacttacggt atgaactttg aatttatgcc ggaacttcat
    14521 ttccaatatg gctaccctat ggcgattggt ttaatgattg ttgccgcctc cgccccttat
    14581 ctttatttta aacgtaaag
//
LOCUS       DANLYI010000045        14365 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.045, whole genome shotgun sequence.
ACCESSION   DANLYI010000045 DANLYI010000000
VERSION     DANLYI010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 14365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14365
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.045"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            complement(260..1393)
                     /locus_tag="PXD81_000985"
     CDS             complement(260..1393)
                     /locus_tag="PXD81_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012478416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine biosynthesis PLP-dependent protein"
                     /protein_id="HDL5537027.1"
                     /translation="MTKFNNIETTLVQIGNRTDPRTGAVAMPIILSTAYGRDGLGEST
                     GWDYTRTKNPTRAVLEQGIADLEGGDAGFAMASGMAAIQLVMSLFKAPDEWIISSDVY
                     GGSYRLFDFSHKNHGTLKPVYVNTADLAAIEAAITPNTKAIFVETPSNPLMEECDVAA
                     IAKIAKKHNLMLIVDNTFLTPALFRPIEHGADIVIHSATKYLSGHNDVLAGLIVAKDS
                     DATKVNGQNLTERLFYFQNCAGAVLSPFDSYLAVRGMKTLALRMERHQSNATELAKFL
                     AEQPEIDSVLYSGKSGMLSFRLQKEEWVAKFLKAIKLITFAESLGGAESFITYPATQT
                     HMDIPEAERITRGITNNLLRFSVGLEHVEDIKADLLQAFKQLK"
     gene            complement(1410..2222)
                     /gene="dapB"
                     /locus_tag="PXD81_000986"
     CDS             complement(1410..2222)
                     /gene="dapB"
                     /locus_tag="PXD81_000986"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005696006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HDL5537028.1"
                     /translation="MTLKIGIVGAGGRMGRQLIQAVHNAQGVELGSAFERKGSSLVGS
                     DAGEVAGVGTLGVVISDDVAQNVAGFDLLIDFTRPEGTLEHLDICVAHHKQMVIGTTG
                     FDDAGKAAIKAASEKIGIVFASNYSVGVNLVFKLLEKAAKVMGDYCDIEVIEAHHRHK
                     VDAPSGTALSMGEHIAKTLGRDLKTHGVFERNGITGERKRDEIGFATIRAGDVVGEHS
                     VWFADEGERVEIAHKASSRMTFANGAVRAAKWLNGKQNGLFDMTDVLDLNNL"
     gene            complement(2273..2650)
                     /locus_tag="PXD81_000987"
     CDS             complement(2273..2650)
                     /locus_tag="PXD81_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5537029.1"
                     /translation="MGIKVLFGTKISAELQYLPEKDLVKILQFKQHVEIYGLENLAGR
                     NKSSDDVPTNDPYWAEKVAKAQKYSLWHYHIGIPEYDTSQGFGNYTSEYILHYVKGEN
                     FIQIVDFTSHPPFKLPSESYLQN"
     gene            complement(2640..3113)
                     /locus_tag="PXD81_000988"
     CDS             complement(2640..3113)
                     /locus_tag="PXD81_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5537030.1"
                     /translation="MMKNLPLSMADTLAVIYPAFDFAADDKPATEQQISLLNFRITMA
                     NQLLIACIETIEKNPNFWAEIDLNDFKKYLNQNKVVIFDTQQYFTDGSRTMAILDNMK
                     SNIRKIEQQLSQATAFNFDLERMKERLNDTFHEVPKDITNVNEFRNWLKGVANGH"
     gene            complement(3181..4269)
                     /gene="rlmM"
                     /locus_tag="PXD81_000989"
     CDS             complement(3181..4269)
                     /gene="rlmM"
                     /locus_tag="PXD81_000989"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015432676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HDL5537031.1"
                     /translation="MFNKLALYCRAGFEKEVAGEITDKAAQQGIFGFANLKENSGYVI
                     FECYQAGEADRLAQALKFEQLIFVRQMLVVGDLLQNLSENDRISPILAQYAELNPRQS
                     SDVFVETPDTNEAKELLTFCRKFTVPLRSALKKQGWLGKSENMQGSVSLHILFTRPGA
                     CYVGYAYNHNKSPFFMGIPRLKFPAEAPSRSTLKLEEAILTFIPQAEEKKRFNDTMVG
                     VDLGACPGGWTYQLVKRGVFVYAVDHGKMAASLHETGRIEHCPEDGFKFQPPKRKQID
                     WLVCDMVEQPIRISKLIAKWLTNGWCRETIFNLKLPMKKRYQEVQLCLNFLEEELTKH
                     GFWFKMQAKHLYHDREEITVHIAVMGQK"
     gene            complement(4269..4661)
                     /locus_tag="PXD81_000990"
     CDS             complement(4269..4661)
                     /locus_tag="PXD81_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HDL5537032.1"
                     /translation="MKNKFVAIAAFSGLFTVAFGAIASHALSAQLSPKALGWIETGIK
                     YQMFHTLAILALGLFQIANFGQNPPVCRAKAFNIIGSNWALGILLFSGNLYLLALNVP
                     TIHWLTPIGGLCFMLGWIALIYISVRKA"
     gene            complement(4768..6169)
                     /locus_tag="PXD81_000991"
                     /pseudo
     CDS             complement(4768..6169)
                     /locus_tag="PXD81_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252330.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
     gene            complement(6324..8681)
                     /locus_tag="PXD81_000992"
     CDS             complement(6324..8681)
                     /locus_tag="PXD81_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin guanine dinucleotide-containing
                     S/N-oxide reductase"
                     /protein_id="HDL5537033.1"
                     /translation="MNTALEKGKFVFLDKPKNTITTAAHWGALNVTVENGKVVKSEGS
                     LAKNHQNELQDVVADQLYSPVRVKYPMVRKGYLEGNKDTTLRGRDKWVRISWDKAFEL
                     VSGEIQRVREQYGSESIFGGSYGWYSSGSLHASRTLLQRYLNVTGGFTNTKGDYSTGA
                     VQVIMPYVLGNIEVYAQQTSWHTIVESTELLVIWSSNPLNTLRIAWTSAEPEGIDYLA
                     KLKASGKRIICIDPVKSETCQFLEAEWLPINTGTDVALILGIAHTLITENKHDHDFLT
                     KYTVGFEQFADYVLGKADNLPKTAEWASQICDIPEEVIKTLANDFVTHRTMLMSGWGL
                     QRLQHGEQSHWALVTLAAMLGQIGLPGGGFGLNYRYSTNGLNVNSGVRLGAINAGKPN
                     PDMPFFPVSRIADALLKPNQPYDYNGQTLHYPNIKLVYWAGGNPFAHHPDTNYLVKAW
                     QQPETMIVNECYWTPTARMADIVLPATTSYERNDLTVAGDYVAKHIIPMKQVVEPQFE
                     AKNDYDIFAELAKRAGKEQAFTEGKSEMEWLKEFYQVAFEAGEKANTPLLDFEAFWAE
                     NKVLSFEAEHQNNTFVRYQDYRTDPIKHKLATPSGKIEIFSETIAKMNYEDCKGHPTW
                     FEHHEYSGKTNEAEPLALVTPHIKYRLHSQLAYSSLRQYYEVNGREPMLIHTKDAADR
                     GITSGDIVRIFNKRGQVLAGAVVTDGIIQGTVALYEGGWYDPQDLGKTDTPLCKNGNP
                     NVLTRNEGSSKLGQGNAPNTTTVQVEKYVGDAPEVTVFKAPFFSE"
     gene            complement(8817..11423)
                     /gene="acnB"
                     /locus_tag="PXD81_000993"
     CDS             complement(8817..11423)
                     /gene="acnB"
                     /locus_tag="PXD81_000993"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014391308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HDL5537034.1"
                     /translation="MENFIENYQQQVDERAALGVVPQPLSAEQTAQLVELLKNPPAEQ
                     AVFLLDLFTNRIPAGVDEAAYVKAAFLADITKGVATSPLISPAHAVKLLGTMQGGYNI
                     ETLLSALDNPELAPIAVEALSKTLLMFDNFHDVKERADQGNPFAQQVLESWANADWFT
                     SRPKLAEKLTVTVFKVTGETNTDDLSPAQDAWSRPDIPLHALAMLKNERDGIFPDENG
                     HTGPIKQLEALKEKGFPLAYVGDVVGTGSSRKSATNSVLWFMGEDIPYIPNKRAGGVV
                     LGGKIAPIFFNTLEDAGSLPIEVDVTNLNMGDVIDIYPYAGKICKHNSDEVLAEFELK
                     TEVLLDEVRAGGRIPLIIGRGLTHKARVALGLPESDVFIKPQSVSESNKGYTLAQKMV
                     GRACGVEGVRPGQYCEPRMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAA
                     YPKPVDVITHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIG
                     ISFPAGSGLVAFAAATGVMPLDMPESVLVRFSGTMQPGITLRDLVHAIPYYAIQQGLL
                     TVEKKGKKNIFSGRILEIEGLEDLKIEQAFELSDASAERSAAACTIKLNKEPIIEYLN
                     SNITLLKWMIAEGYDDKRTLERRIANMQAWLDNPELLEADENAEYAAVIEINLDEIKE
                     PIVCVPNDPDDARLLSQVQGDKIDEVFIGSCMTNIGHFRAAGKLLSQAQGELPTKLWI
                     APPTKMDAGLLTEEGYYSIYGKSGARVEMPGCSLCMGNQARVANNANVVSTSTRNFPN
                     RLGQGANVYLASAELAAVSALLGKLPTPQEYLDYMNNLQKDKDDIYRYMNFDQINSYV
                     KKADNVIVQVAV"
     gene            complement(11443..12690)
                     /gene="icd"
                     /locus_tag="PXD81_000994"
     CDS             complement(11443..12690)
                     /gene="icd"
                     /locus_tag="PXD81_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HDL5537035.1"
                     /translation="MQTQVQIPQGETIQLNQDGSLQVPDNPIIPFIEGDGIGVDVTPA
                     MKAVLDAAVEKAYQGKRKIAWMEIYAGEKANKLYGENTWLPDETLELIRQYHVAIKGP
                     LMTPVGGGIRSLNVAMRQGLDLYNCLRPIRYYDGTPSPVKHPELVDMVIFRENSEDIY
                     AGVEWVAGSPEANKVIEFLQQEMGVKKIRFTEDCGIGIKPVSKQGTQRLVRAALQYVI
                     DNNRKSLTLVHKGNIMKFTEGAFKEWGYQVAEEFGAKLIDKGPWMSLTNPKTGEEIII
                     KDSIADAFLQEILLHPTEYDVIATLNLNGDYISDALAAQVGGIGISPGANIGFEAAIF
                     EATHGTAPKIAGQNKGNPGSLILSGEMMLRHLGWVEAADLVVKAVSKTIANKTVTFDF
                     AEMLEGVTLRSTSEFAQDIIANM"
     gene            12924..14204
                     /locus_tag="PXD81_000995"
     CDS             12924..14204
                     /locus_tag="PXD81_000995"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005762304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HDL5537036.1"
                     /translation="MSKATLTLETGEKLELNMQKGTLGYQTIDIQPFLKHKLFAYDPG
                     LVSTAVCRSNITYVDGDEGVLLYRGYPIDQLAKHSDYLEVGYMLLFGERPTKEEYKDF
                     VQLIKKHTLVHEQLTKFFSGFRRDSHPMAVMCGVSGALAAFYHDAIDVNNQAHRELTA
                     IRLLAKIPTLAAMCYKYSIGQPFMFPQNHLSYAGNFLYMMFATPCEPYEVNPVLERAL
                     DRIFILHADHEQNASTSTVRTAASSGANPFACIAAGIASLWGPAHGGANEACINMLKE
                     IGTVDRIPEFIARAKDKNDPFRLMGFGHRVYKNYDPRAKVMRETCHEVLKELNIDNPL
                     FEVALELERIALSDPYFIEHKLYPNVDFYSGIVLKAIGIPTSMFTVMFALARTVGWIA
                     HWKEMYLQGNIKIVRPRQIYTGHTKRDFEPMDKS"
BASE COUNT         4124 a         3315 c         2747 g         4179 t
ORIGIN      
        1 gctaaaaaat ttgaacaaac gaagcgacag cgttagcaaa aacttaaaat gatgtacgga
       61 tttaaaaatc tgttcgtttg cttgttttat aagcaaacta atttcgcata attattatta
      121 tgttaaatag atagttaaaa taacacattt aattaaatct tttcgtgttt ctctcctcct
      181 ttattaaaaa agaaaacggt cagattggta aaatctatac ctaatatgac cgcttgtgaa
      241 tacaaaaaaa ttacttcaat tacttcaact gtttaaaggc ttgcagtaaa tcagctttaa
      301 tatcttcaac gtgttctaaa ccaacggaaa aacgcagtaa attattcgta atgcctcgtg
      361 taatacgttc tgcttccgga atatccatat gggtttgtgt tgcaggataa gtaataaaac
      421 tttctgcacc gcctaaactt tccgcaaagg taatcaattt aattgccttg agaaatttcg
      481 ctacccactc ttctttttgt aaacggaaag ataacatacc acttttgcct gaatataaca
      541 cgctgtcaat ttcaggttgc tctgctaaga atttagccag ttcggtggca ttagactgat
      601 ggcgttccat tcgtaatgcc aaggttttca taccacgcac ggcaaggtaa gaatcaaatg
      661 gcgacagcac tgcaccggca cagttttgga aatagaataa acgctcggtc aaattctgcc
      721 cgtttacttt ggtagcatca gaatctttcg caacaattaa gccggctaac acatcattat
      781 gccccgaaag atacttggtc gcactgtgaa tcacaatatc agctccgtgc tcaatcggac
      841 ggaataacgc tggggttaag aacgtattat caacgatcaa cattaaattg tgttttttcg
      901 caattttagc aatggcagca acgtcgcact cctccatcaa tggatttgac ggcgtttcca
      961 cgaaaatcgc cttagtattc ggtgtaatag ctgcttcaat cgctgctaaa tccgctgtat
     1021 taacataaac cggctttaat gtgccgtgat ttttgtggct aaaatcaaac aagcggtaag
     1081 aaccaccata aacatcgctg gaaataatcc attcatcagg tgctttaaat aatgacatta
     1141 ctagctgaat cgctgccatt ccagatgcca tcgcaaaacc ggcatcaccg ccttctaaat
     1201 ccgcaatccc ctgctctaat acagctcgag ttgggttttt ggtacgggtg taatcccagc
     1261 cggtagattc gcctaagcca tcacgcccgt aagcagttga taagataatc ggcattgcca
     1321 ccgcccctgt acgaggatca gttctgttac cgatttgcac taatgtggtt tcaatattat
     1381 tgaattttgt cattgttatt atccttcaat tacagattat ttagatccaa tacgtctgtc
     1441 atatcaaaca agccattttg tttgccattt aaccattttg cagcacgcac tgcgccattg
     1501 gcaaacgtca ttcgactaga ggctttatgt gcgatttcga cacgttcgcc ttcatcagcg
     1561 aaccatacac tatgctcccc tacaacatca ccggcacgaa tagtagcaaa gccgatttca
     1621 tcacgtttgc gttcgcctgt aataccgtta cgttcaaata cgccatgagt tttcaagtca
     1681 cgcccaagag ttttggcaat atgttctccc attgataaag ccgtacctga tggtgcatca
     1741 actttatggc gatggtgtgc ctcaatcact tcaatatcac aataatcgcc cattactttt
     1801 gctgcttttt cgagcagttt aaacaccaga ttcaccccaa cgctgtagtt tgaggcaaaa
     1861 acgataccga ttttttcact cgctgctttg attgcagctt tacctgcatc atcaaaaccg
     1921 gttgtgccaa tcaccatttg tttatgatga gccacacaaa tatccaaatg ttctagcgtg
     1981 ccttcagggc gagtaaaatc aattagtaaa tcaaaacctg ctacattttg ggcgacatca
     2041 tcagaaatca ccacacctaa tgtaccaacc ccggctactt caccagcatc actacctact
     2101 aatgatgaac ctttacgctc aaaagcagac cctaattcta cgccttgtgc attatggacg
     2161 gcttgaatta attgacgccc cattcgccca ccggcaccga cgattccaat ttttaatgtc
     2221 atatttgttc ttccttttta tatttgtttt ttacaagcgg ttacattttt aattaatttt
     2281 gcaaatagct ttctgatggc aatttaaaag gtgggtgaga agtaaaatca acaatttgaa
     2341 taaagttttc acctttcaca taatgtaaaa tatactcaga agtataatta ccaaatcctt
     2401 gactggtatc atattctgga atgccgatat gataatgcca taaagaatat ttttgtgctt
     2461 tagccacctt ctctgcccaa taaggatcat tcgttggcac atcatcagaa cttttatttc
     2521 gaccagctag attttccaat ccataaattt caacgtgttg tttaaactgc aggattttga
     2581 ctagatcttt ttcaggcaaa tattgcaatt cggctgaaat ttttgtacca aataaaactt
     2641 taatgcccat ttgcaacacc tttcaaccag tttctaaatt cattaacatt cgttatatct
     2701 ttaggcactt cgtgaaaagt atcatttaac cgctctttca ttcgctctaa gtcaaaatta
     2761 aatgcggttg cttgagagag ttgctgttca attttgcgaa tattggactt catattatcc
     2821 aatatagcca ttgtccttga gccatcagta aaatactgtt gggtatcgaa aatcaccacc
     2881 ttattttgat ttaggtattt tttgaaatcg tttaaatcaa tttcagccca aaagtttgga
     2941 tttttttcga tagtttcaat acaggcaatt aataattgat ttgccattgt aatacgaaaa
     3001 tttaacaatg agatttgctg ctctgttgca ggtttatcat cagctgcgaa atcaaatgcc
     3061 ggataaatca ctgcaagcgt gtcagccata gataagggta aatttttcat catctctcct
     3121 tacggaagaa gttgtcatta tctactggat tacttaaaaa aacaacttat tcattttatg
     3181 ctatttttgc cccatcaccg caatatgtac cgtaatttcc tcacggtcgt ggtagaggtg
     3241 tttagcttgc attttgaacc aaaacccgtg tttcgttagc tcttcttcca agaaattgag
     3301 gcatagttgc acttcttgat aacgtttttt catcggcagt tttaggttga aaatcgtttc
     3361 tcgacaccaa ccgttagtca gccattttgc aatgagtttc gagatacgaa tcggctgctc
     3421 taccatatca caaaccagcc aatcgatttg tttacgctta ggcggttgga atttaaaccc
     3481 atcttcaggg caatgctcaa ttcttccggt ttcgtgtaaa cttgctgcca ttttgccgtg
     3541 atcgactgca tagacaaaaa caccacgttt gactaattga taagtccaac cacccggaca
     3601 agccccaaga tcgaccccta ccatcgtatc attaaagcgt tttttctcct ctgcttgcgg
     3661 aataaaagtt aaaatcgcct cttctaattt aagcgttgag cgactaggtg cctcagccgg
     3721 gaattttaaa cggggaatcc ccataaaaaa cggcgattta ttatggttgt acgcatagcc
     3781 cacatagcaa gctcccggac gagtaaataa aatatgcaag ctcacactac cctgcatatt
     3841 ttccgatttc cccaaccaac cttgtttttt caatgccgac cgtaatggca cagtaaattt
     3901 acgacagaaa gttaataatt ctttcgcttc gttggtatcc ggtgtttcga caaatacatc
     3961 agaactttgg cgaggattaa gttcggcata ttgggctaaa atcggcgaaa ttcgatcatt
     4021 ttcagacaga ttttgcaaca aatccccaac aaccaacatt tggcgaacaa aaataagctg
     4081 ttcaaacttt aacgcttggg ctaatcgatc tgcttcccct gcttgatagc actcaaaaat
     4141 cacatagcca ctattttctt taaggttggc aaagccaaaa atgccctgtt gtgcagcttt
     4201 atccgtgatt tcgcctgcta cctctttttc aaaccctgct cggcaatata aggctaattt
     4261 attgaacatt aggcttttct cacactgata taaattaacg caatccaacc aagcataaag
     4321 cagagaccac caataggcgt aagccaatga atagtcggca catttaacgc cagtaagtac
     4381 aggttgccac taaaaagcag aatgcctaac gcccaattgc ttccgatgat gttaaaggct
     4441 ttggctctac aaacgggtgg attttgccca aaatttgcaa tttggaataa acctaaagct
     4501 aaaatggcaa gggtgtgaaa catttgatat ttaatccccg tctcaatcca gcctaaggct
     4561 ttaggcgata attgagccga taatgcgtgg ctggctatcg ccccaaatgc cacagtaaat
     4621 aagccgctga atgcggcaat tgctacaaat ttgtttttca taagttgaag ataggtatga
     4681 tgttagatgg cttactatat catattatcg aaaccaacaa gcggtgaaat tttgcaaaaa
     4741 aattacaaaa aatcaccgtt tgtatgctta atggttcaag ataatatcca catcttccga
     4801 tttatcgtga accccaaatc tatctactaa ggttttgctg gcctcattta atccgataat
     4861 ttctacctca acatcacttt ttcgatattt caacacaact ttatctaacg ctgaaactgc
     4921 cgttacgtcc caaaagtgag cgtgggttaa atcaatgtgg acttttttca gttctgaacg
     4981 ggtaaaatca aattgtgcga taaaatcatc agatgaggca aaaaatactt gcccgataac
     5041 atcataacgc aattcctctt tctctatagc acttgtttta accgccatat aacgtccaat
     5101 tttattcgca aagaataatg ccgagagcaa cacgccggaa aatacaccaa gggctaagtt
     5161 atgtgtataa accacaattg caacagtgac taacatcact acattaaagc cgattgggtg
     5221 cgtttttagc tcttttaagg aatgccaatt aaaggtgccg atagagacca taatcatcac
     5281 tgcaactaat gcagccatcg gaatcactct caaaatatcg ctcaaaaaga ccacaaaaat
     5341 cagtaataat attggcaatg ccctgccctt tacattcacg gtttttatcg ctgtctgtct
     5401 ctgtcatttc atcaacaatc gttgccgtca ttaaagattc caataaccct actgcggcca
     5461 agctgaaaga ataaggcaaa ataatccata aggtgtctaa attaagcgga atatcgggta
     5521 ataaaaacac cggtaaggta tcgggtaatt gccccatatc cccaacggta cgaacatcaa
     5581 tgccttgcca taatgtgaaa agtgttaaag caataatggt cactaatggt gagggaatga
     5641 tttttccaat caccggtaca taaggaaaaa gataaatgat agcaagccct gctaccacca
     5701 gtgcataagt atgccaagtt gtattttgta actcaggtag ttgtgccata aaaattaaaa
     5761 tggcaagtgc attaacaaac ccaatcacta cggctcgtgc cacaaaccgc ataagcgagc
     5821 cgagttttaa ccaaccggca ataatttgca acacgcctgt taataaggtg gccgctagca
     5881 tatattgtaa accgtgatcc ctcactaagc ctgctaatac cagagccatt gcaccggttg
     5941 ctgcagaaat catcgccggt ctgccgccca caaaactgat aattactgca atacaaaatg
     6001 aggcatataa accaatttgt ggatcaaccc ctgcaataat tgaaaatgct atggcttctg
     6061 gaattaacgc caaagccacc actaaaccag agagaacatc acctcgtaca tttgaaaacc
     6121 attgttgttt aattaccgta ggcgaaaatt gctgaaataa agttgtcatt attaccctta
     6181 aataaaaaga aaagccgagc aaagtcggct aaaaatgagg cgagagagta gcaaattagt
     6241 gaaaagtcgt caaataaaca agcggtcttc tttcgctctt tttttgcaaa aaatagtaga
     6301 aaaatgaccg cttggattaa ttgctattca ctaaaaaaag gggctttaaa caccgtcact
     6361 tcaggtgcat ctcccacata tttttccact tgaaccgtgg tagtgttcgg tgcattgccc
     6421 tgcccgagtt ttgatgaacc ctcattacgg gttagcacat tcgggttgcc gtttttgcat
     6481 agtggagtat ctgttttgcc taaatcttgc gggtcatacc aaccaccttc gtataacgct
     6541 actgtgcctt gaatgatgcc atcagtcacc actgctcctg ccaacacctg ccctcgcttg
     6601 ttaaaaattc gcacaatatc accgcttgta atgcctctgt cagcggcatc ttttgtgtga
     6661 attagcattg gttctctgcc gtttacctcg taatattgac gtaatgagga gtaagcaagc
     6721 tggctgtgta aacggtattt aatatgcggt gtaactaatg ccaacggctc ggcttcgttg
     6781 gttttgcctg aatattcgtg atgttcaaac caagtcgggt gtcctttaca atcttcataa
     6841 ttcatcttgg caatggtttc ggagaaaatc tcaattttgc ctgatggcgt tgccaatttg
     6901 tgtttaatcg gatcagtacg ataatcttga tagcgaacga aagtattatt ttgatgttct
     6961 gcctcaaagc tcaacacttt attttccgcc caaaatgcct cgaaatccag caatggcgta
     7021 ttagcttttt cccccgcctc aaaggcaact tggtaaaatt ccttaagcca ttccatttcg
     7081 cttttgcctt cggtaaatgc ttgttcttta ccggctcgtt tcgctaattc ggcaaaaata
     7141 tcgtaatcat tcttcgcctc aaattgcggt tcaaccacct gtttcatcgg gataatatgt
     7201 tttgctacat aatcaccggc aacggtgaga tcgtttcgct cgtagctggt ggttgcgggc
     7261 aatacaatat ctgccattcg agcggtcggt gtccagtaac attcattgac gatcatcgtt
     7321 tccggctgtt gccacgcttt cactaaatag ttggtatccg ggtggtgagc aaacggatta
     7381 ccccccgccc aatagactaa tttgatgttc gggtaatgta atgtttgccc gttgtaatca
     7441 taaggctgat tcggtttgag caacgcatcg gcaattcgag acaccgggaa aaacggcata
     7501 tcggggttag gtttacctgc attgattgcc cctaaacgaa caccggaatt aacatttagc
     7561 ccattggtag aataacgata atttaagccg aaaccgccgc ccggcaagcc aatttgcccg
     7621 agcatagcgg caagagtcac taacgcccag tggctttgct ccccgtgttg caggcgttgc
     7681 aagccccagc cggacatcaa cattgtgcgg tgagtaacaa aatcatttgc taaggtttta
     7741 atcacctcct ccggaatgtc acaaatttgg ctcgcccact ctgctgtttt aggtaaatta
     7801 tctgcttttc ctaacacata atcagcaaat tgctcgaaac caacagtata tttagttaag
     7861 aagtcgtgat cgtgcttgtt ttcagtaata agggtgtggg cgatccctaa aatcagtgca
     7921 acatccgtgc cggtattaat aggcaaccac tctgcctcta aaaattgaca ggtctcgctt
     7981 ttcaccgggt cgatacaaat aatccgttta ccgcttgctt ttaatttggc gagataatca
     8041 atcccctccg gttcggcaga ggtccacgca atgcgaagtg tattcagtgg attagacgac
     8101 caaattacca ataattctgt gctttctaca attgtgtgcc aactggtttg ttgagcatac
     8161 acctcaatat tgcctaatac ataaggcata atcacttgca ccgcaccggt ggaataatcg
     8221 cctttagtat tggtaaagcc gcctgtgacg tttaaataac gttgcaaaag cgtgcgagag
     8281 gcgtgcagtg aaccactgct ataccagccg taagaaccgc cgaaaatgga ttcgctgcca
     8341 tattgctctc ttacccgttg gatttcgccg gaaaccagct caaaggcttt gtcccaagag
     8401 attctcaccc acttatctcg ccctcgcaaa gtggtgtctt tattcccttc caagtagcct
     8461 ttccgcacca tcggatattt cacccgaacc gggctataga gctgatccgc caccacatct
     8521 tgcaattcgt tttggtgatt tttcgctaaa ctgccctctg atttcaccac ttttccgttt
     8581 tccaccgtta catttaatgc accccagtgg gcggcggtgg taatggtatt tttcggttta
     8641 tccaaaaaga caaacttccc tttttctaat gctgtgttca tttaaattaa tgccttttct
     8701 gttacaaaat tgagaggaat ttactggatt aagccaaaag ttgcaaccac aagcggttgt
     8761 ttttagccaa aaatttgcaa aacaaaaccc acctaaaaag gtgggcttca aggaagttaa
     8821 acggcaactt gaacgattac attgtccgct tttttcacat aactattgat ttggtcaaag
     8881 ttcatatagc ggtaaatatc gtccttatct ttctgcaagt tattcatata gtctaggtac
     8941 tcttgcggtg tcggcaattt acccaataac gcagaaactg cggctaattc tgctgaggct
     9001 aaatacacat ttgccccttg ccctaaacgg ttcgggaagt ttcgggtaga ggtagaaacc
     9061 acattagcat tatttgccac tcgggcttgg ttacccatac agagagaaca gcccggcatc
     9121 tctacccttg ctccgctttt gccgtaaatg ctgtaatagc cctcttcggt cagcaagcct
     9181 gcgtccattt tagtcggcgg agcaatccat aatttagtcg gcaattcgcc ctgtgcttgg
     9241 cttaacagtt tacctgccgc acggaaatgc ccgatattag tcatacaaga accaataaag
     9301 acttcatcaa ttttatcgcc ctgtacttga gaaagcaggc gagcatcgtc agggtcattc
     9361 ggtacgcaca caatcggctc tttgatttca tctaagttga tctcaatcac tgcggcatac
     9421 tccgcatttt cgtctgcctc taaaagctcc ggattatcca accacgcttg catattggcg
     9481 atacggcgtt ctagcgtgcg tttatcgtca tagccttccg caatcatcca ttttaaaagc
     9541 gtaatattag aattcagata ctcgataatc ggctctttgt tgagcttgat ggtacaggct
     9601 gcagcagagc gttcagcaga agcatcagaa agctcgaagg cttgttcgat tttcagatct
     9661 tccaaccctt caatttctaa aattcgaccg gagaaaatat ttttcttacc ttttttctcc
     9721 accgtcagca agccttgctg aatggcataa tacggaatag cgtgaaccag atcccgtaag
     9781 gtaatgcccg gttgcattgt gccggaaaaa cgcaccaaga ccgattccgg catatctaac
     9841 ggcatcaccc ctgttgccgc agcaaaggca actaagcccg aaccggcagg gaatgaaatc
     9901 ccgattggga aacgggtgtg ggaatcgccg cctgtaccga cagtatccgg caataacata
     9961 cggtttagcc aagagtgaat cacgccatca cccgggcgaa gtgacacacc gccacggttc
    10021 ataataaaat caggcaaagt gtggtgggta attacgtcca ccggtttcgg gtaagcggca
    10081 gtatggcaga acgattgcat cacaagatct gccgagaagc ctaaacaggc taaatctttc
    10141 agctcatctc ttgtcattgg tcctgtggta tcttgtgaac cgacagacgt cattcgaggc
    10201 tcgcaatatt gccccggtcg aacgccttct acgccacaag ctcgccctac cattttttga
    10261 gcaagggtgt agcctttgtt actctctgaa accgactgcg gtttaataaa tacatcactt
    10321 tcaggcaagc caagtgctac tctggcttta tgggtcaaac cacgtccgat aatcagcgga
    10381 attcgcccgc ctgcacgcac ttcatcaagc aatacttcgg tttttagctc aaattcggct
    10441 aagacttcat cggagttgtg tttgcaaatt ttgcctgcat aagggtaaat gtcgattaca
    10501 tcgcccatat tcaggttggt tacatccact tcgatcggta aagaaccggc atcttctaat
    10561 gtgttaaaga aaatcggggc aattttaccg cctaacacca ccccaccggc acgtttgttt
    10621 ggaatgtaag gaatatcttc ccccataaac caaagcacgg agttggttgc ggatttacga
    10681 gaagagccgg tgccgactac atcaccgaca taggctaacg ggaaaccttt ttcttttaat
    10741 gcttccagtt gcttaatcgg gccggtatga ccgttttcat cggggaaaat accgtcacgt
    10801 tcatttttca gcattgctaa tgcgtgaagt ggaatatcag ggcgagacca agcgtcttga
    10861 gccggggaaa gatcatcggt attggtttcg cctgtgactt taaataccgt taccgttaat
    10921 ttttcggcaa gtttcgggcg agaggtaaac caatcggcat tcgcccaaga ttccaatact
    10981 tgttgtgcaa acggattacc ctgatctgcc cgttctttta catcgtggaa attatcaaac
    11041 atcaataatg ttttagataa ggcttctacc gcaatcggtg caagctcagg gttatccaac
    11101 gcagacaata aggtttcaat gttataacca ccttgcattg tgccgagtaa tttcactgcg
    11161 tgagcaggcg aaatgagcgg cgatgtggca acccctttgg taatatcggc taaaaaggcg
    11221 gctttaacat aagcggcttc atcaacacct gccggaatac gattggtaaa aagatccaat
    11281 aaaaagaccg cttgttcagc cggtgggttt ttcaataatt caaccagttg agccgtttgt
    11341 tccgcagaaa gtggctgagg cacaacaccc agtgccgcac gttcatcaac ctgttgttga
    11401 taattttcta taaaattttc catcatcatt ccctctccaa ctttacatat tggcaataat
    11461 gtcttgtgca aattctgagg ttgagcgtag agttaccccc tccagcattt ccgcaaaatc
    11521 aaaggtgaca gttttattgg caatggtttt agacaccgct tttaccacta aatcagccgc
    11581 ttcaacccaa cctaaatggc gtaacatcat ttccccactt aaaattaatg acccaggatt
    11641 gcctttattt tgccctgcaa ttttaggggc tgtgccgtgt gtggcttcaa aaatagccgc
    11701 ttcaaaccca atattcgctc ccggtgaaat accgattccg cctacttgtg cggctaaagc
    11761 atcagaaata tagtcgccgt ttaggttcag tgtagcaatc acatcatatt cggttgggtg
    11821 taacaagatt tcttgtaaaa aggcatcggc aatgctgtct tttataatga tttcctcacc
    11881 ggttttcgga ttagtcaagc tcatccaagg gcctttatca attaattttg ccccaaattc
    11941 ttccgcaact tggtagcccc actctttaaa tgctccttcg gtaaatttca taatgttacc
    12001 tttatgcaca agcgtgagcg atttacggtt gttatcaatt acatattgca atgcagcacg
    12061 caccaagcgt tgtgtgcctt gcttagaaac cggcttaatt ccgataccgc aatcttcggt
    12121 aaagcggatt tttttcacgc ccatctcttg ttgtaaaaat tcgatcactt tattggcttc
    12181 aggcgaaccg gcaacccatt ctacaccggc ataaatatct tccgagtttt cacggaaaat
    12241 caccatatcc accagctcag ggtgtttaac cgggcttggc gtaccgtcat aataacgaat
    12301 tgggcgtaag cagttgtata aatctaatcc ttgacgcatc gccacattta aagaacgaat
    12361 accgccaccc accggtgtca ttaaaggacc tttaatcgcc acgtgatatt ggcggataag
    12421 ctctaaggtt tcatccggca accaagtgtt ctcaccatat aatttattcg ccttttcgcc
    12481 tgcgtaaatt tccatccaag caatttttcg cttgccttga taggcttttt ctaccgccgc
    12541 atctagcact gctttcattg caggagtaac atcaacaccg atgccgtcac cttcaataaa
    12601 tggaataatc ggattatccg gaacttgtaa agaaccgtct tggttcagtt gaatagtttc
    12661 gccttgtgga atttggactt gtgtttgcat tgattttacc cccgtaatga cgaaaaagta
    12721 gtgacgaaaa atgaatgttg acaatcagcc aacaatggat taactataaa cagtaaattt
    12781 ctaaatgtaa ataaagcaaa atgggctgag agagaatttg tgatctaacg cacatttatg
    12841 taaataaaat gtaaaaaatc gttgtgattc ttttggaaat catctagttt atcttataaa
    12901 tctcaaataa ataggagtaa gctatgtcaa aagcaacatt aacactcgaa acaggtgaaa
    12961 aacttgaact caatatgcaa aaaggaacct tagggtatca aacgattgat attcagcctt
    13021 tcttaaagca taagttattt gcttatgatc ccggattggt ttcgaccgct gtatgccgat
    13081 ccaatatcac ttatgttgat ggcgatgaag gggttttact ctatcgaggc tacccgattg
    13141 accaattagc caaacattct gattacttag aagtcggtta tatgctgtta ttcggcgaac
    13201 gccctaccaa agaagaatac aaagacttcg ttcaactcat caaaaaacac actcttgtac
    13261 acgaacaact aaccaaattc ttctccggtt tccgccgaga ttcgcaccca atggcggtga
    13321 tgtgtggggt aagtggagct ttggctgcct tctaccacga cgccattgat gtaaacaacc
    13381 aagcccaccg tgaattaacc gccattcgtt tgttagccaa aatcccaacc cttgcggcga
    13441 tgtgttataa atactctatc ggtcagccgt ttatgttccc acaaaaccac ttatcttacg
    13501 ccggcaactt cttatatatg atgtttgcca ctccttgtga accgtacgaa gtgaaccctg
    13561 tgttagagcg tgcgttagac cgaattttca ttttacatgc agaccacgaa caaaacgctt
    13621 cgacctcaac cgtgcgaacc gccgcttcat caggtgcaaa cccatttgct tgcattgctg
    13681 ccggtattgc ctctctctgg ggacctgcac acggaggagc aaatgaagcc tgtatcaata
    13741 tgttgaaaga aatcggcact gtggatcgca ttcctgaatt tattgctcga gccaaagata
    13801 aaaatgaccc gttccgctta atgggctttg ggcatcgtgt ctataaaaac tacgaccctc
    13861 gtgcgaaagt aatgcgtgaa acctgccacg aagtgttaaa agaactgaac attgataacc
    13921 cactatttga agtggcattg gaattagaac gtattgcgtt aagcgaccct tatttcattg
    13981 agcataaact ttatccaaac gtagatttct actccggaat cgtgctaaaa gccatcggta
    14041 ttccaacctc aatgtttacc gtaatgtttg ccctagccag aaccgttggc tggattgctc
    14101 actggaaaga gatgtatctc caaggcaata tcaaaatcgt tcgtccacgt caaatctaca
    14161 ccggacatac taaacgtgat tttgagccaa tggataaatc ataagattgg caaaaaatcg
    14221 aaaaatttta accgcttgtt tccacaagcg gttatatttt gcataaaatt tgcaaatcag
    14281 gctaatttat ttagcagttg tgttctaaca tcttgaccat tttcatcgct ttttacagcc
    14341 ataataaata agccttttcc ttgac
//
LOCUS       DANLYI010000046        14295 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.046, whole genome shotgun sequence.
ACCESSION   DANLYI010000046 DANLYI010000000
VERSION     DANLYI010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 14295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14295
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.046"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            1..342
                     /locus_tag="PXD81_000996"
     CDS             1..342
                     /locus_tag="PXD81_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5537037.1"
                     /translation="MRLPDDEFTFIKTFASAHKLTLQNFVMSAIQAKVAEMSAKNKIS
                     EESNIHQPLSKDEKIMLNEAKVFYSARSHLKAENVILPSDFMEQIKSGCGAEKMAEMM
                     GYTKEDWESMK"
     gene            344..667
                     /locus_tag="PXD81_000997"
     CDS             344..667
                     /locus_tag="PXD81_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5537038.1"
                     /translation="MGQITFSNIFTKVIFENVLSASTKEKIKQMLFQSVVALPPLHSE
                     RKMILQLKKQKITIFYQVIEEQSVQILAYQFGGTDKLTGVSKAHFKTLDAIFQMTDEE
                     KEGKF"
     gene            complement(677..1009)
                     /locus_tag="PXD81_000998"
     CDS             complement(677..1009)
                     /locus_tag="PXD81_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HDL5537039.1"
                     /translation="MQIEQYQQWVRQFYQQQGWYERSPFMRVTYLAEEIGEVACAVRA
                     IEIGRERPDETEASQQQKRDNLIEELGDVMDNLFVLADKYGISMTEVLERHQAKFEKR
                     YLQKNMEK"
     gene            complement(1102..2298)
                     /locus_tag="PXD81_000999"
     CDS             complement(1102..2298)
                     /locus_tag="PXD81_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009875146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HDL5537040.1"
                     /translation="MNIPFCLPENISPETFLREYWQKRPLLIRNGLPQIVGLFEPEDI
                     LELALEEEVTARLIQCENEKWSVKSSPLVEEDLEELPVQWSVMVQNLEQWSPELGELW
                     QAFGFIPQWQRDDIMVSCSPLNGTVGKHYDEYDVFLVQGYGQRRWQLGKWCDPSTEFK
                     PNQPIRIFDDMGELVMDEVMNPGDVLYVPSRMAHYGVSESEQGLTFSFGLRYPNATDL
                     LENLCKTIEQQADNINSSEFHLPFRLTPNEQPNALLDPKMVKMFKHQLIDLLQNSEQF
                     DELLTHSVATAASSRRYELLQTDNEYYPDEVQEILEAGGWLQQDANVKILYTENPQRV
                     YVNGEWIDELNEAEMALLIRIANGDSISWNKLASKVQDQAELELMLDTICDWLDSGWI
                     ILSEAE"
     gene            complement(2447..3790)
                     /gene="accC"
                     /locus_tag="PXD81_001000"
     CDS             complement(2447..3790)
                     /gene="accC"
                     /locus_tag="PXD81_001000"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015431873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HDL5537041.1"
                     /translation="MLEKVVIANRGEIALRILRACKELGIKTVAVHSTADRELKHVKL
                     ADETICIGPAPSVKSYLNIPMIIAAAEVTNADAIHPGYGFLSENANFAEQVEKSGFIF
                     IGPTADVIRLMGDKVSAINAMKKAGVPCVPGSDGPVGNDPKKNKEIAKRIGYPVIIKA
                     SGGGGGRGMRVVHHEKDLEESIAMTKAEAKAAFNNDMVYMEKYLENPRHIEIQVLADT
                     HGNAVYLAERDCSMQRRHQKVVEEAPAQGITPELRQFIGERCANACREIGYRGAGTFE
                     FLYENGEFYFIEMNTRLQVEHPVTEMITGVDLVKEQLRVAAGLPLSITQDDIKVKGHA
                     IECRINAEDPNTFLPSPGKITRLHVPGGLGVRWDSHIYSGYSVPPHYDSMIGKLITFA
                     ENRDIAIRRMENALSETIVEGIKTNIPLHTQILADKEFRKGGTNIHYLEKKLGLK"
     gene            complement(3862..4329)
                     /locus_tag="PXD81_001001"
     CDS             complement(3862..4329)
                     /locus_tag="PXD81_001001"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HDL5537042.1"
                     /translation="MDIRKIKKLIELVEESGITELEVSEEEGTVRISRAAPAVAPAAV
                     QYAAAPVVAPAAPQVASTVPVAAPAEAPKAAETSGHAVLSPMVGTFYRSPSPDAKPFV
                     EVGQTVKIGDTLCIVEAMKMMNRIESDKAGVVKEILVNDGEPVEFDQKLFIIE"
     gene            complement(4582..5364)
                     /locus_tag="PXD81_001002"
     CDS             complement(4582..5364)
                     /locus_tag="PXD81_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005609325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HDL5537043.1"
                     /translation="MAVKNEQFIVICNGVFRLFGNIVKILSYPFHALFPKKRFTIPEY
                     SPAKIQSSIPSKITKTIWQTNYSNKVTLPMYANYLFNRLMSLSYDYRYVSTEEREAYI
                     KANADERTFNAYSKLTDGAAQADFWRIFTLLREGGIYIDIDGHLVYPLSKIIGENDSE
                     VLIKRRDKYTNFFLASEAGNWILKDTLELIISNIENRRIEGGVFVMTGPDTLNNAIGD
                     KEVNFRRDKVTCAQGTFTNEYFQYIDKPGSKWNYKKNEDLLK"
     gene            complement(5374..5811)
                     /gene="aroQ"
                     /locus_tag="PXD81_001003"
     CDS             complement(5374..5811)
                     /gene="aroQ"
                     /locus_tag="PXD81_001003"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HDL5537044.1"
                     /translation="MKKILLLNGPNLNMLGKREPQIYGSQTLADIENHLKKLAAAQDV
                     ELESFQANGEEPIINRIHQAFQHTDFIIINPGAFTHTSVAIRDALLSVSIPFVEVHLS
                     NVHSREPFRHHSYLSDIAKGVICGLGAKGYEYALDFAISHLKA"
     gene            complement(5897..6265)
                     /locus_tag="PXD81_001004"
     CDS             complement(5897..6265)
                     /locus_tag="PXD81_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL5537045.1"
                     /translation="MAFTIRKMVEQDTHQVFPLMQKLAVFEHYIDSFAITPEVVMESG
                     FRKSPPDFYCLVAENASNIIGILVYYFLPYTAQNRPAIYMKELYVDETARNQKVGEAL
                     MQALKNEAKAHHCKTIKSLE"
     gene            complement(6325..7149)
                     /gene="dapD"
                     /locus_tag="PXD81_001005"
     CDS             complement(6325..7149)
                     /gene="dapD"
                     /locus_tag="PXD81_001005"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018650734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HDL5537046.1"
                     /translation="MSLQAIIEAAFERRAEITPKTVAAETKAAVEEVIAGLDNGSLRV
                     AEKKDGEWVVNQWVKKAVLLSFRIADNEIIDGAETKFYDKVPTKYGNYTEEQFKADGI
                     RAVPGAVVRKGSHIEKNVVLMPSFVNIGAYVGEGTMVDTWVTVGSCAQIGKNVHLSGG
                     VGIGGVLEPLQANPTIIGDNCFIGARSEIVEGVIVEDNCVISMGVFIGQSTKIYDRET
                     GEVHYGRVPAGSVVVSGSLPSKDGSHNLYCAVIVKKVDEKTLSKVGLNDLLRSIDE"
     gene            7297..7959
                     /locus_tag="PXD81_001006"
     CDS             7297..7959
                     /locus_tag="PXD81_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HDL5537047.1"
                     /translation="MENFANLLTGKTREHLVPLPNALSDKHYLQPQVVEAFLTLQHAA
                     KQAGFNLQPASTFRDFERQKGIWNAKFRGERKVHDDNGVAIDMAALSDLEKCKAMLRW
                     SAVPGASRHHWGTEIDIFDPDLLPENTPLLLEPWEYLEGGYFADLTKWLHVNAETFGF
                     YFPFDGKHSRVGFEPWHISYRPISNEYEQRFNDEILKAAWQDEEVAGKECLLEHFEEL
                     LK"
     gene            7956..8708
                     /locus_tag="PXD81_001007"
     CDS             7956..8708
                     /locus_tag="PXD81_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDL5537048.1"
                     /translation="MIQLINETQQHDKFRQICDKWQLVEDKSSPLALVLTEERLELRK
                     LDEPKLGAIAVNFVDGTMAHRRKFGGGRGEAVAKAVGIKGDYLPTVIDATAGLGRDAF
                     VLAAVGCKVLLVERNPIVAALLEDGLNRAYQDVEIGSFMQERMILADVRDISQLDPQS
                     QMAEVVYLDPMYPHKQKSALVKKEMRVFQHLVGADLDSDSFFLPAKGLAKKRVVVKRP
                     DYAPFLAEQKPDFSHTTKNHRFDVYLSHLKND"
     gene            8799..9635
                     /gene="ftsN"
                     /locus_tag="PXD81_001008"
     CDS             8799..9635
                     /gene="ftsN"
                     /locus_tag="PXD81_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005613437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HDL5537049.1"
                     /translation="MPQRDYVARSNEKKSKKNQKESNVKKILMRIIGVILFFGIGLWF
                     LKSSAPVPTVPSAPQPANSQTKSELPSRPEETWSYIRDLETRTVITDNSQTSQERLAQ
                     LTAEQKQQIEERRKQEEARLAAEQAQQKPATDVSATELAPKTEEVKSLTEEELAAKKA
                     QEQALAQKQAEEKRRQAELKKQQEAKKEQPAKIVASAATLADKAANQAGKFGLQCGAF
                     KNKAQAENMQARLAMAGFNARIASSADWNRVFVGPIGDRAATSRAQSNARSVAECVIV
                     AM"
     gene            complement(9677..10858)
                     /locus_tag="PXD81_001009"
     CDS             complement(9677..10858)
                     /locus_tag="PXD81_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HDL5537050.1"
                     /translation="MKTELDMIVIGGGMIGAATALGLAKQGLRLALLEKNPLPTFNPD
                     SDYDLRISAISMGSIQLLEKLGVWEQIVAMRSTPYTQLETWEIDGFNTLFSAADLGLE
                     KLGDMVENNVIQIALWEQLKQYPNCLQAVGFEQIFANKAGEYWEVTVDGNTFAAPLLI
                     AADGANSQVRQWAGIGLTAWQYRQHCLLATVTTEKPAPSGTWQQFYPTGPRAFLPLSG
                     NHGCVVWYDSPQKIAELKMLPAEKLGVAIEQHFPSRLNAELGNIQVQASGAFPLTRQH
                     AQHYVQNGVVLVGDAAHTINPLAGQGVNLGFKDVKTLLEVIEQAVKKGKNFASEQVLQ
                     RYEQKRKPDNLLMQTGMDVFYKAFKTDLLPLKIARNVGLVLADKVSPIKKQALKYAIG
                     I"
     gene            10965..11303
                     /locus_tag="PXD81_001010"
     CDS             10965..11303
                     /locus_tag="PXD81_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5537051.1"
                     /translation="MSCKTHANHEHEHGEGCGHTAIKHNDHIDYLHDGHLHHNKHGDH
                     YDEHVLEVNETNPADCNAAHCCGDHVHGEVCGHEAVPHGDHIDYIVNGRLHHQHGDHC
                     DDHGPVEIVK"
     gene            11435..12304
                     /locus_tag="PXD81_001011"
     CDS             11435..12304
                     /locus_tag="PXD81_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HDL5537052.1"
                     /translation="MTLAIVYSRASIGVEAPLVTIEVHISNGQPKLTIVGLPEATVKE
                     AGDRVRSALLNANFIYPPQRITINLAPADLPKEGGRFDLPIAIGILAASGQIDSDLLT
                     QFEFLGELALTGHLRGVHGTIPAVISADKAKRQMILAKQNANEASLVSNATTYFAGSL
                     LEVVNMLNKRDKLPICQHISQHSAEIRPLVSRDLTDIIGQQHAKRALMIAAAGQHNLL
                     FLGPPGTGKTMLASRLADLLPEMTDEEAIETASVTSLVQNELNFQNWKQRPFRSPHHS
                     ASMVALVGGGCEN"
     gene            12396..14207
                     /locus_tag="PXD81_001012"
     CDS             12396..14207
                     /locus_tag="PXD81_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012478332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5537053.1"
                     /translation="MFYLNNDNFKREPYRGYCPSHLEKLFTKPHLDIRGNGYYYPDNE
                     YVVAWYESDFIFKSRKAQAHAKVKLVNLTCPKYTGKNSAEYLSVLYISVPLNYLHYFT
                     IGSIWKGGVAKEQFALEEFYITVEAENQDGKRENLSIVSFGESEENGLEKPFDYTIYT
                     IPTEYHNYGDDLNRLLSISYQNQKFIIHPLHIFMMHYGYSTDIKRILATYPLDEVRER
                     LFIDKVVENFDVERYVVLPKYFVKKDAIFLYHFKYDEETTGTRVKKLVSQFRLNIHNQ
                     THPIEIDFWHTQKVELKIRGVRLGNAVLCAEIIGLNQPEGEDITLVLSQSKREKSGNK
                     TEEQNNEVNNVPITRVYTREPELDELPLTDNSPDNRTVEYNKRQFELLGRQRQIRALK
                     KAREESNQKMKALTPDEIDSLGVGESDGRNGKVGLAFCFLDDTPVGKSSKLYKLWQHA
                     KAVTEWNHGEAHWYTPNLGFRNDDELLPVSLETNKCFDYPEIAIIIRLQIYGETFFLI
                     DFSMKQRDISIRGLGYKPDPNEDFLYESDMSKSELSELLSAVHHHEHLPKEYIEKKNK
                     EKTKLTTFNHSEAESSNWAYNAVQKLTSRAITKPTYF"
BASE COUNT         4171 a         3024 c         3049 g         4051 t
ORIGIN      
        1 attagattgc cagacgatga atttactttt attaaaacat ttgcttctgc tcataaactg
       61 actttacaaa attttgtgat gtccgcaatc caagctaaag ttgcagaaat gtctgctaaa
      121 aataaaatat ctgaagaaag taacattcat caacctcttt ctaaagatga aaaaataatg
      181 ctaaatgagg caaaagtatt ttattctgct cgttcgcatt taaaggcaga aaatgtgatt
      241 cttccgagtg attttatgga gcaaattaaa tcaggctgtg gagcagaaaa aatggcagaa
      301 atgatgggat ataccaaaga agattgggag tcaatgaagt aatatggggc agattacatt
      361 tagtaatatt ttcacaaaag tgatttttga gaatgtgctt tctgcttcaa ctaaagagaa
      421 aataaaacaa atgctttttc agtctgtggt tgcattacct cctctccatt cagaaagaaa
      481 aatgatttta cagttgaaaa agcaaaaaat taccattttt tatcaagtta ttgaggaaca
      541 atctgtgcaa atcctcgctt atcaatttgg tggaactgat aaattgacag gtgtgtctaa
      601 agctcacttt aaaacactag atgccatttt tcaaatgacg gatgaagaga aagaaggaaa
      661 attctaatgt aagcggttat ttttccatat ttttttgcaa ataacgtttc tcaaattttg
      721 cttgatggcg ttcaagcact tccgtcatcg aaatgccata tttatcggct aatacaaaaa
      781 gattatccat cacatcgcct aactcttcga ttaagttatc tcgtttttgc tgttgtgaag
      841 cttcggtttc gtccggtcgt tcacgcccga tttcaatcgc tcttactgca caagccactt
      901 cgccgatttc ttctgctaga taagtcaccc gcataaaagg gcttcgttca taccaacctt
      961 gttgttggta aaactggcga acccattgtt gatattgttc aatttgcatt ttatttctga
     1021 ttcaaaaagt agggacgaaa aatttttcgc ccttttataa gaaaatgtat cagggcaaat
     1081 gatgatttgc ccctaggagt gttactctgc ctcagagaga ataatccagc cactatccag
     1141 ccagtcgcaa atggtgtcta gcattagctc cagctccgct tggtcttgca ctttggaggc
     1201 aagtttgttc cacgaaatcg aatctccatt ggcgatacga atcagtaatg ccatttctgc
     1261 ttcgtttaac tcatcaatcc actcgccatt tacataaacc cgttgagggt tctcggtgta
     1321 gaggattttt acatttgcat cttgctgtaa ccagccgccg gcttcgagaa tttcttgcac
     1381 ttcgtcaggg taatattcat tgtcagtttg caataattca taacggcggg agcttgccgc
     1441 agtggcaaca ctgtgagtga gtaactcatc gaactgttcg gaattttgga gcaagtcgat
     1501 taactgatgt ttaaacattt tcaccatttt cggatcaagc aacgcattcg gctgttcatt
     1561 cggcgttaag cggaacggaa gatgaaattc cgatgaatta atgttgtcgg cttgttgttc
     1621 gatggttttg cagagatttt ccagtaaatc ggtcgcattt ggataacgta aaccaaaaga
     1681 gaaagttaaa ccttgctcgc tttcggaaac accatagtga gccattcgag acggcacata
     1741 aagcacatca cccgggttca ttacctcgtc cattaccagt tcgcccatat catcaaaaat
     1801 gcggatcggt tggtttggtt tgaattcggt gcttggatcg caccatttgc ctaattgcca
     1861 acgacgttgc ccatagcctt gcaccaagaa cacatcgtac tcatcataat gtttgccgac
     1921 agtgccgttg agcggtgagc aagaaaccat aatgtcatca cgttgccatt gcgggataaa
     1981 accaaaagcc tgccaaagct cgcctagctc aggagaccat tgctcgaggt tttgcaccat
     2041 tacagaccat tgtacaggta actcttccaa atcttcctca acaagcgggc tggatttaac
     2101 agaccatttc tcgttttcgc attgaatcag gcgtgcagtg acttcttcct ctaatgccag
     2161 ttctaaaata tcttccggct caaacaatcc gacaatttgg ggtaagccgt tacgaattaa
     2221 tagcggtcgt ttttgccaat attctcgcaa aaaggtttca gggctgatat tttcaggtaa
     2281 gcagaatgga atgttcataa tggctccttg ctacgtgctt ctttgagatt actgatattc
     2341 tacccgagaa atcaggaaaa acgggaaatt ttttatgatt tagcgacatc attcacattt
     2401 tgaagaggaa ataatgaaaa aggcgaacat tgttcgcctt tgaaatttat tttaaaccga
     2461 gttttttctc gagatagtgg atattggtgc cacctttgcg gaattcttta tccgcaagaa
     2521 tttgagtatg taaaggaata ttggttttaa tgccttccac aatggtttcg gaaagggcat
     2581 tttccatccg gcgaatcgca atatcacgat tttcagcaaa ggtaatcaat ttaccaatca
     2641 ttgaatcgta atgtggcggc acgctatagc ctgagtaaat atgtgaatcc caacgcacac
     2701 ctaaaccacc cggaacgtgt aaacgggtaa ttttacccgg cgatggcaag aaggtgtttg
     2761 gatcttctgc gttgatacgg cactcaatgg cgtgaccttt cactttaata tcatcttgtg
     2821 tgatcgaaag tggtaagccg gctgccacac gcagttgctc ttttaccaag tccacaccgg
     2881 taatcatttc ggttacaggg tgttctacct gtaagcgggt gttcatttca atgaagtaaa
     2941 actcgccgtt ttcatataag aactcaaaag tacctgcacc acggtagcca atttcacggc
     3001 aggcattggc acaacgctcc ccaataaatt gacgcaattc aggtgtaata ccctgagcag
     3061 gtgcttcttc caccactttt tggtggcgac gttgcattga acagtcacgc tctgctaaat
     3121 aaaccgcatt accgtgagta tcggcaagta cttggatttc aatatggcgt ggattttcta
     3181 aatatttctc catatacacc atatcgttat taaatgccgc tttcgcttcg gctttcgtca
     3241 ttgcgataga ttcttccaaa tctttctcgt gatgaaccac acgcatacca cgaccgccac
     3301 caccacctga tgctttgatg atcaccgggt agccgatacg tttagcgatt tctttatttt
     3361 tcttcggatc gttaccaacc ggaccgtcag aacccggtac gcaaggcacg ccggcttttt
     3421 tcattgcatt gatagcagag actttatcgc ccattaagcg aatcacatca gccgttggac
     3481 caataaagat aaagcctgat ttctcaactt gttcggcaaa gtttgcgttt tcagataaga
     3541 aaccgtagcc cggatgaata gcatcggcat tcgttacttc ggcagcggca ataatcatcg
     3601 ggatatttaa atagcttttt accgatggag caggaccgat acaaatggtt tcatcggcta
     3661 atttaacgtg ttttaattca cggtcggcag ttgaatgcac tgcaaccgtt ttaatgccca
     3721 attctttaca agcacgcaaa atacgcaggg caatttcacc acggtttgcg ataacaactt
     3781 tttctaacat agggaaattc cttgtgttct agaaaatgta gggcgtgttg cacacgcccg
     3841 ttggataatt taaggaagag cttattcaat gatgaataat ttttggtcga attctaccgg
     3901 ttcgccatca ttaactaaga tttcttttac cacaccggct ttgtctgact cgatacggtt
     3961 catcattttc attgcttcta caatacataa cgtatcaccg attttcacgg tttgacccac
     4021 ttcaacgaac ggttttgcat ctgggcttgg gctacggtag aaagtcccca ccattggtga
     4081 aagcactgcg tggccactcg tttctgcggc ttttggtgct tcagccggag ctgcaacagg
     4141 tactgtcgaa gctacttgag gtgccgctgg tgcaacaaca ggagctgctg catattgaac
     4201 tgcagctggt gcaacagctg gagcagcacg gctaatacgc accgtacctt cttcttcaga
     4261 cacttctaac tcggtaatgc cagattcttc aactaattca ataagttttt tgattttgcg
     4321 aatatccata ataagattcc gtatgtgatt aaataaaata aaaaagagaa ctccacattt
     4381 ggggtataaa ttcaaatgta gagttaaaaa atgctgggcg tagaatacct gaatttcgct
     4441 aattttgcga ataaaatctg ataattttgg cgaaaatgga cgattttagc attcatcgta
     4501 ccagttagga tttttggaaa atggtgactc acaagcgatt gaaaattgcc aattttttgt
     4561 aaaaaacaac cgcttgtcag attatttcaa taaatcttca tttttcttgt aattccactt
     4621 gctaccgggc ttgtcgatat actgaaaata ttcgttggta aaagtacctt gagcacaagt
     4681 tactttgtct cggcggaaat ttacttcttt atcaccaatg gcattattta gggtgtcagg
     4741 accggtcata acaaacacgc caccttcaat ccgacgattc tcaatattgc tgataatcag
     4801 ctcaagggtg tcttttagaa tccaatttcc agcttccgaa gccaagaaaa agttggtgta
     4861 tttatcacgg cgtttaatta acacttcgct atcattttcg ccaataattt tagagagtgg
     4921 gtaaaccaaa tgcccgtcaa tatcaatata aatgccacct tctcggagca aagtaaaaat
     4981 tcgccaaaaa tcagcttgtg ccgcaccatc ggttaatttg ctataggcgt taaaggtgcg
     5041 ttcatcggcg ttggccttga tgtatgcctc tcgttcttct gtgctaacgt agcgataatc
     5101 gtagctcaat gacattaggc ggttaaacag ataattcgca tacattggca aggtgacttt
     5161 gttagaataa ttcgtttgcc aaatggtttt ggtaattttg ctcggaatgc ttgattggat
     5221 ttttgccggg ctatattccg gaatggtgaa acgttttttc ggaaaaagtg catggaacgg
     5281 ataactcaat attttgacga tattaccgaa taaacggaag actccgttac agatcacaat
     5341 aaattgttca tttttaacag ccattttatt accttatgct tttaagtgtg aaattgcaaa
     5401 atccaacgca tattcatagc cttttgcccc taaaccgcaa ataacgcctt ttgcaatatc
     5461 acttagatag gagtgatggc gaaaaggttc acggctatga acgttagaca aatgcacctc
     5521 aacaaacgga atcgacacac tcaataacgc atcacgaatt gccacgctgg tgtgagtaaa
     5581 tgccccgggg ttaataataa taaaatcggt atgctgaaag gcttggtgaa tacggttgat
     5641 aatcggctct tcaccgttgg cttgaaagct ctccagctca acatcttggg ccgcagccaa
     5701 tttttttaag tggttttcaa tatccgccaa agtttgcgaa ccgtaaattt gtggctcacg
     5761 tttgcccaac atatttaaat tcgggccgtt aaggagaagg atttttttca tatttactta
     5821 ttcaacttgt aattcataat taagccattc acggttttct ttcgccccca agcgttgata
     5881 aaaacgaata ccaccgtcat tccaaggatt ttattgtctt acaatgatgt gccttcgcct
     5941 catttttcaa ggcttgcatt aacgcctctc ccactttctg attccgagca gtttcatcaa
     6001 cataaagctc tttcatataa atagcggggc ggttttgggc agtgtaagga agaaaataat
     6061 aaaccaatat gccgataatg ttagacgcat tttcagccac taaacagtaa aaatcgggtg
     6121 ggcttttgcg aaagccggat tccattacaa cttcaggagt aatcgcaaaa gaatcgatat
     6181 agtgctcaaa cacagccagt ttttgcatca gagggaacac ttggtgggta tcttgttcga
     6241 ccattttgcg gattgtaaat gccataaaat tccttacaag cggtaaaaaa atccctcact
     6301 tttgcaaagc gagggaggtt ttaattattc gtcaatagaa cgtaataagt cgtttaagcc
     6361 cactttgctt aaggttttct catccacttt tttcacaatc accgcacagt agaggttgtg
     6421 tgaaccatct tttgatggaa gcgagcctga aaccacaaca gagcctgccg gcacacggcc
     6481 gtaatgtact tcgccggttt cacggtcgta aattttggta gattgaccga taaacacgcc
     6541 cattgagatc acgcagttat cttcgacaat cacgccttct acgatttcag aacgtgcacc
     6601 gataaagcag ttgtcgccga tgatggttgg gttcgcttgt aatggctcta acacaccacc
     6661 gatgcccacg ccaccagaaa ggtgaacatt tttaccgatt tgcgcacaag aacccactgt
     6721 tacccaagtg tccaccattg tgccttcgcc aacgtatgca ccgatgttca cgaaagatgg
     6781 cattaacacc acgtttttct cgatatgtga acctttacgc accaccgcac ccggcacggc
     6841 acgaatgcca tctgctttga attgctcttc ggtgtagttg ccgtatttgg ttggcacttt
     6901 gtcgtagaat ttggtttctg caccgtcgat gatttcattg tcggcaatac ggaatgaaag
     6961 taataccgct tttttcaccc attggtttac tacccactcg ccatcttttt tctcggcaac
     7021 acgcagtgaa ccgttgtcta atcctgcgat cacctcttct actgctgctt tggtttcagc
     7081 agcaacggtt ttcggagtaa tttccgcacg gcgttcaaat gccgcttcaa taatagcttg
     7141 taaagacatt gttgtgttcc ttcaatatat aaattaaaac agaaaattcg gctcactttt
     7201 taacatagaa aggcgtttat ttccacaaga aaaaacatat cgcagaaatt tagtttataa
     7261 ttccaagcca cttatttgat acaagcggtg atttttgtgg aaaattttgc aaatctgctt
     7321 accggtaaaa cgcgtgagca tttagtgccg ttgccgaacg ctctttcgga taaacattac
     7381 cttcagcctc aggtggttga ggcttttttg acgttgcagc atgctgcaaa acaagccggc
     7441 tttaatttac agcctgccag tacttttcgt gattttgaac gccaaaaagg gatttggaat
     7501 gcgaagttta ggggtgagcg taaggttcac gatgataacg gggttgcgat tgatatggcg
     7561 gcgttatcgg atcttgaaaa gtgcaaagca atgttgagat ggtctgcggt gccgggggca
     7621 agtcggcatc attggggaac ggaaattgat atttttgacc ctgatttatt gccggaaaat
     7681 acgcctttat tgcttgagcc gtgggaatat ttggaaggtg gctattttgc cgatttaacc
     7741 aagtggttac acgttaatgc ggaaacattt ggcttttatt tcccgtttga cggtaaacat
     7801 tctcgagtgg gctttgagcc gtggcacatt agctatcgcc cgatttcaaa tgaatatgag
     7861 caacgcttta acgatgaaat cttaaaagct gcgtggcaag atgaagaagt ggcgggtaag
     7921 gaatgcttac ttgaacattt tgaagaatta ctaaaatgat ccaattaatt aacgaaaccc
     7981 aacaacacga caaattccgc caaatttgcg acaaatggca gctggttgaa gataaatcca
     8041 gcccgttagc cttagtgcta acggaggaac gattggaact acgcaaatta gacgaaccca
     8101 agctcggggc gattgcagtg aattttgtgg acggtacgat ggcacatcgg cgtaaattcg
     8161 gtggtgggcg aggtgaagcc gttgcgaaag cggtcggcat taaaggggat tatttgccaa
     8221 ctgtgattga tgcgacagcc ggtttagggc gagatgcttt tgtattagca gcggttggtt
     8281 gtaaagtgct gttagtggaa cgcaatccaa ttgtcgctgc cttgttggaa gatggtttaa
     8341 accgagctta tcaagatgtg gaaatcggca gttttatgca agaaagaatg atcttagccg
     8401 atgttcgaga catttctcag ctagatccac aatcccaaat ggctgaggta gtgtatttag
     8461 atccgatgta tccgcacaaa caaaaaagtg cgttggtgaa aaaagaaatg cgggtgtttc
     8521 aacatttagt tggggcagat ttagattcgg acagcttttt cttaccggca aaaggattgg
     8581 caaaaaaacg tgtggtagtg aagcgtccgg actatgcccc gtttttagcg gagcaaaagc
     8641 ctgatttttc acacaccacc aaaaatcacc gttttgatgt ttatctttct cacctgaaaa
     8701 atgattaaag gtgggtcatg cgataggcaa gcggttattt ttacagtaaa atttacaaat
     8761 tatgttatcg tttatttata tttaaaaagg cggttatcgt gccacaacgt gattatgtcg
     8821 ctcgttccaa cgagaaaaaa agtaaaaaaa atcaaaaaga aagtaatgtt aaaaagattt
     8881 taatgcggat tatcggggta attttattct tcggtattgg tttatggttt ttgaaatcca
     8941 gtgcacctgt accaacagtt ccgtcagcac cgcagcctgc taattcacag actaaaagcg
     9001 aattaccaag tcgaccggaa gagacgtgga gctatattcg tgatttagaa acccgtacgg
     9061 taattaccga taatagccaa acttcacaag aacgtttggc acaattaacg gcggaacaaa
     9121 agcagcaaat tgaagaaaga cgcaaacaag aagaagcacg cttagccgct gagcaagctc
     9181 aacaaaagcc tgcgaccgat gtatctgcta cagaattggc tccgaaaaca gaggaagtta
     9241 agtcgttaac ggaagaagaa ttagcagcaa aaaaagcaca ggagcaagct ttagcacaaa
     9301 agcaagcgga agagaaacgc agacaagcag agcttaaaaa acagcaggaa gcgaaaaaag
     9361 agcagccggc taaaattgta gcaagtgctg caacactggc agataaagct gcaaatcagg
     9421 cgggtaaatt tgggttacag tgcggtgcat ttaaaaataa agcccaagcc gaaaatatgc
     9481 aagcaagatt ggcaatggca ggttttaacg cccgtatcgc aagcagtgcc gattggaacc
     9541 gtgtttttgt cggcccgata ggtgacagag cggcaacttc cagagctcaa tccaatgcac
     9601 gcagtgtagc ggaatgtgtg attgtggcga tgtaaaattg attgaaatat tgaagagagc
     9661 tataggctct ctttttttat atcccaatcg catatttcaa ggcttgcttt ttgatgggtg
     9721 acactttatc agccaatact aaaccgacat tgcgggcaat tttgagcggt aataaatcgg
     9781 tcttaaacgc tttataaaac acgtccatgc ctgtttgcat cagtaaatta tccggtttac
     9841 gtttttgctc atagcgttga agcacctgct cacttgcaaa atttttgcct tttttgaccg
     9901 cttgttcgat cacctcaagc aaggttttaa catctttaaa gcctaaattt acaccttgtc
     9961 cggcaagcgg attaatggtg tgagccgcat cgccgactaa taccaccccg ttttgcacat
    10021 agtgctgagc gtgctggcgg gttagcggga aagcccctga agcctgcacc tgaatgttgc
    10081 ctaattctgc gttgagccgg cttgggaaat gttgctcaat cgcaactccc agcttttctg
    10141 ctggaagcat tttcagttcg gcaatttttt gtggtgaatc gtaccaaaca acgcagccgt
    10201 gattgccgga aagcggtaaa aaggctcgag ggccggttgg atagaattgt tgccaagtgc
    10261 cgcttggagc aggtttttcg gtggtaacgg tggcgagtaa acagtgttgg cgatattgcc
    10321 aagcggttaa accaatgccc gcccattggc gaacctgtga atttgcaccg tctgcggcaa
    10381 tcagcagtgg ggcagcaaag gtgttgccat caaccgtcac ttcccaatac tcgcccgctt
    10441 tatttgcaaa aatttgctca aatccgaccg cttgtaggca gtttggatat tgctttaatt
    10501 gctcccataa cgcaatttga ataacgttgt tttcgaccat atcgccgagc ttttccaaac
    10561 ccaaatcagc tgccgaaaaa agagtgttga atccatcaat ttcccaggtt tcgagctggg
    10621 tataaggggt gcttctcatt gcaacaattt gctcccacac gcccaatttt tctaacagtt
    10681 gaatcgagcc catactaatg gcggaaattc gcaaatcgta gtctgaatca ggattaaagg
    10741 tcggcagagg gtttttctcc aacaaggcta aacgcagccc ctgttttgct aagcctagag
    10801 ctgttgctgc cccaatcata ccgccaccga ttacaatcat atctaattca gttttcattt
    10861 tttcttcctt tttagttagg caaaatactg ctaaaacgcc ttgaattgta gtgcatttca
    10921 gattaggatc aaaccgattt atctatttat ctaaggagcg ttttatgtct tgcaaaactc
    10981 atgcaaacca cgagcacgaa cacggcgaag gctgtggtca tactgctatt aaacataacg
    11041 accatatcga ctatttacac gatgggcatt tacaccacaa caaacacggc gatcactatg
    11101 acgaacacgt tttagaagtg aatgaaacca acccggcaga ctgtaatgcc gcccattgct
    11161 gcggcgacca cgttcatggt gaggtttgcg gacacgaagc tgtgccgcac ggcgatcata
    11221 tcgactacat cgtgaatggg cgtttgcacc accaacacgg cgaccactgc gatgaccacg
    11281 ggccggttga aattgtgaaa taagattttt gatgggaggt tttagcctcc cattattatt
    11341 tttgcgatct cgctcgaaaa attgaacatt cctgctaccc ctcttccgat tttcctttta
    11401 tgctctcggt attgatttct aataccgaaa tgtgatgacc ttagcgattg tttacagccg
    11461 agcctccatt ggcgttgaag ccccgttggt gacgattgaa gtgcatatta gcaacggaca
    11521 accaaaatta accattgtcg gcttgcccga agccacagtg aaagaggctg gtgatcgtgt
    11581 gcggagtgcg ttgctgaacg ctaattttat ctatccgcca caacggatca cgattaatct
    11641 tgctccggca gatttaccca aagaaggcgg acgttttgac ttgccgattg cgattggcat
    11701 tctggcagcc tcagggcaaa ttgattctga tttattaacg cagttcgagt ttttgggcga
    11761 gttggcactg acagggcatt tgcgaggagt gcacggcacg attccggcag tgatttctgc
    11821 cgataaagcc aaacggcaga tgatcttagc caagcaaaat gcgaatgagg catcgcttgt
    11881 ttccaatgcc accacctact ttgccggctc gttgttggag gtggtaaata tgctcaacaa
    11941 acgggataaa ctgccgattt gtcagcacat ttcgcaacat tctgccgaaa ttcgaccgct
    12001 tgtcagccga gatcttactg atattatcgg gcaacaacac gccaagcgag ccttaatgat
    12061 tgccgctgcc gggcaacata atttgctgtt tctcggacca ccgggaacgg gtaaaacaat
    12121 gttggcaagc cggctggcgg atttactgcc ggaaatgacc gatgaggagg cgattgaaac
    12181 cgcctcggtg accagccttg tgcaaaatga gctgaatttt cagaactgga aacaacgccc
    12241 gtttcgttcg ccccatcaca gtgcatcaat ggtggcatta gtgggaggtg gctgtgaaaa
    12301 ctagaaataa atgtcaaaaa caagaaataa atgtcacaaa tctttctcta atttcgctat
    12361 aattcgatca tcaaaacttt aaggggaaat ccgttatgtt ctacctcaat aacgacaatt
    12421 tcaaacgaga accttatcgt ggatattgtc cgagtcacct tgaaaagctt tttactaaac
    12481 cacaccttga tattagaggt aacggttatt attaccctga caatgaatat gttgtagctt
    12541 ggtatgaatc ggacttcatt ttcaaatcaa gaaaagctca agcacatgca aaagtaaagc
    12601 ttgttaattt aacttgcccc aaatacactg gcaaaaacag tgctgaatat ttaagcgttt
    12661 tgtatatctc cgttcccctt aattatctac attatttcac tatcggcagt atctggaaag
    12721 gcggtgtcgc caaagagcag tttgctctcg aagaatttta cattacggtt gaagctgaaa
    12781 atcaagatgg taaacgagaa aacctctcaa tcgtttcatt cggtgagagt gaggaaaacg
    12841 gtttagagaa accatttgat tacacaatct acactatccc cacagaatat cacaactacg
    12901 gtgatgacct aaaccgacta ctttccatct cttaccaaaa tcaaaaattc attatccatc
    12961 cgctacatat ttttatgatg cactacgggt actctacgga catcaagcgt atcttggcaa
    13021 catacccact tgacgaggta agagaacgcc tatttataga taaggtcgtt gagaattttg
    13081 atgtagagcg ttatgtcgtt ttaccgaaat actttgtaaa aaaagatgcg attttcctct
    13141 atcacttcaa atatgacgaa gagactacgg gaacaagggt aaaaaagctt gtaagccagt
    13201 ttaggttaaa tattcacaat caaacacatc caatagaaat agatttttgg cacacgcaaa
    13261 aggttgaatt gaaaatccgt ggtgttcgac ttggaaatgc tgtgttatgc gcagaaatta
    13321 tcggtcttaa tcagcccgaa ggcgaagata ttacgctagt gctatctcaa tcgaaaagag
    13381 aaaaatcggg taataaaacc gaggaacaaa ataacgaggt aaataacgtt cctattacca
    13441 gagtttatac acgagaacct gaattggatg agttaccact caccgataac tccccagata
    13501 atcgaacagt cgaatacaac aagcgacaat ttgaactctt gggccgtcaa cgccaaatca
    13561 gagcgctcaa gaaagcacgc gaagagagta atcagaaaat gaaagccttg actccagatg
    13621 agatagactc actgggtgta ggtgaatcag atggccgaaa cggtaaggtt ggactcgctt
    13681 tctgtttcct tgatgataca cctgttggca aaagctccaa actatacaaa ttatggcagc
    13741 acgcgaaggc tgttacggaa tggaatcacg gtgaggcgca ctggtacacg cctaatctcg
    13801 gttttagaaa tgacgatgaa ctccttcccg tttccttaga aaccaacaaa tgtttcgact
    13861 accctgaaat cgcgataatt atccgcctcc aaatatacgg agaaacattt ttcctgattg
    13921 atttctcaat gaagcaaaga gatataagca ttcgtggctt agggtataaa cccgatccaa
    13981 acgaagattt tctctatgaa agtgatatga gcaaaagcga attatccgaa ttattgtccg
    14041 cagttcatca tcatgaacat ctgccaaaag agtacataga gaaaaagaac aaagaaaaaa
    14101 ccaaactcac tacatttaac catagcgaag ccgaaagtag taactgggct tataacgcgg
    14161 tgcaaaaatt aacttcaagg gcaattacta agccgacata tttttaatta tttttatgaa
    14221 tgcagaacaa cgtgtaattg ttactccgac attttggggt aaattttttg gaaaaattaa
    14281 aagtgttgaa cttca
//
LOCUS       DANLYI010000047        13966 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.047, whole genome shotgun sequence.
ACCESSION   DANLYI010000047 DANLYI010000000
VERSION     DANLYI010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 13966)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13966)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13966
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.047"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            complement(2..2191)
                     /gene="yccS"
                     /locus_tag="PXD81_001013"
     CDS             complement(2..2191)
                     /gene="yccS"
                     /locus_tag="PXD81_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01666 family membrane protein"
                     /protein_id="HDL5537054.1"
                     /translation="MNKFLRYLQHSWLSENVIKTLPMFISLNIAAVSIWQLGISDFAM
                     PLMLGIIAGGLVDLDNSLIGRAKNLIISLVAFSLSSIGASVSLHLGWLFVPVAVVCTF
                     LLVMLGAIGQRYSTIAFGTLIVAVYNSLAYTPEVLWYHNVVMILLGTLLYGLVGILVY
                     LIFPNRTVQENLASAYLALSHYLLAKSAYFDPDDDDLAAKQLALAKANSEVMAAFDKT
                     RVALFYRLSRQHKQQRTQRMLRYYFTAQDILERASSSHYQYHELFQQLSNSDLMFRFQ
                     RVLELQAAACQKIAMSLRHREVYEHGVRGEKALQGLLNSLNYHQERGLKSAYRWRAIA
                     ENLGNIERQLAQIEQGSSSGEHIEPLSKNFTKSHRLIAENITGLSNISQAIKAHLTFE
                     SQLFRHAVRLSIVVLLCSAIVPILGFDSKGYWILLTAIFVCQPNYTATKTRLIQRVIG
                     TIFGVLVGGSFELFNVALSLEAQLGLIVLTGSLYTFFRLVHYGFSTFYITVLVMISLD
                     IAGVSLESGILMRMLDTLLGTAIAWLAVAFLWPDWKYLNLQRNLHNGLKASGQYLRHI
                     IAQLQFGYNEQLPYRIARRDVHNHLSALSNAITNMYSEPQKYANDLTFAPKLLGIAYT
                     LLGYISTLGAYRIASREINQQVDFSAMFFSHAREVANIIGQIASESRSFAKLSEKLTA
                     IDQDLTQFEQENQQKQNDLALVLIQQLRLIVQLLPQLNSILQLKSVR"
     gene            complement(2235..2693)
                     /locus_tag="PXD81_001014"
     CDS             complement(2235..2693)
                     /locus_tag="PXD81_001014"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HDL5537055.1"
                     /translation="MKTTVRTIAKQKQIALVAHDSCKQDLIRWTQTHQAQLASHKLYA
                     TGTTGHLLARETGLEITSLLSGPMGGDQQLGGLIAEKKIDLLVFFWDPMNAAPHDPDV
                     KALMRIATVWNIPVAINETTANFLLNADLFNQAISVNVPDYDGYLAERLG"
     gene            2819..4093
                     /gene="thrC"
                     /locus_tag="PXD81_001015"
     CDS             2819..4093
                     /gene="thrC"
                     /locus_tag="PXD81_001015"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HDL5537056.1"
                     /translation="MNLYNIKHPEEQVNFAQAVRQGLGKNQGLFFPEVLPKLDSIDEL
                     LDLPLVKRSQKILSALIGEELPQETLNAMVKNAFTFPAPLAKVNDDIYALELFHGPTL
                     AFKDFGGRFMAQALANVRGDGKITILTATSGDTGAAVAHAFYGLENINVVILYPKGKI
                     SPLQEKLFCTLGGNIRTVAIESDFDACQALVKQAFDDAELRQAIGLNSANSINISRLL
                     AQICYYFEAVAQLPKEKRDNVVVSVPSGNFGNLTAGLIAKTLGLPIKRFIASTNANDT
                     VPRYLQSGKWEPNATVATLSNAMDVSRPNNWPRVEELFKRNGWSLSELGSAALNDAET
                     EAALKAQYAEGYLCEPHGAIAYQSLKDQLQQGETGIFLCTAHPAKFKESVEHILAIEL
                     PLPEALDKHNKLPLLSDEMAADFATLRAYLLK"
     gene            complement(4172..4906)
                     /locus_tag="PXD81_001016"
     CDS             complement(4172..4906)
                     /locus_tag="PXD81_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011848602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HDL5537057.1"
                     /translation="MSFKDMTGQKVPSVTFRTRQNDAWVDVTTDELFNNKTVVVFSLP
                     GAFTPTCSSTHLPRYNELACEFKALGVDDIICMSVNDTFVMNAWKADQESENITVIPD
                     GNGEFTEGMGLLVDKNDLGFGKRSWRYSMLVKNGIVEKMFIEPQEPGDPFKVSDADTM
                     IKYLSPNWTAKESVSIITKPGCLFCAKAKALLKEQGYTFEEIVLGRDASTISVRAITG
                     KTSVPQVFIGGKYIGGSDELAAYFAK"
     gene            5021..5905
                     /gene="oxyR"
                     /locus_tag="PXD81_001017"
     CDS             5021..5905
                     /gene="oxyR"
                     /locus_tag="PXD81_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HDL5537058.1"
                     /translation="MNIRDLEYLIALAEHKHFRRAAESCNVSQPTLSGQIRKLEGELG
                     TILLERTSRKVLFTQAGLTLVEQAKVVLREVKILKEMASNQGKEMSGPLHVGVIPTLG
                     PYISPIIVPALKAEFADLELYIYELQTNQLLDQLESGQLDCGIISFVKESESFIEVPI
                     FNEEMYLVVPEQHEWAALKKFDMNLLKNRELLFLDSGHCLRTNTLDYCLSIGAKENPH
                     FKATNLETLRNMVAANVGLALIPKLAAKPIKGVHYIPLENPKPFRAVGMVYRPGSPLR
                     ARYERLAQAITKIMQSEA"
     gene            5908..6540
                     /gene="fabR"
                     /locus_tag="PXD81_001018"
     CDS             5908..6540
                     /gene="fabR"
                     /locus_tag="PXD81_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HDL5537059.1"
                     /translation="MANGMSVRAIQKEKTRRALVEAAFNQLSSEKNFSNLSLREVARE
                     AGVAPTSFYRHFKDMDELGLAMVDESGLLLRQLMRQARKRIANGGSVVVVSVETFFEL
                     IADRPNVFQLLLRESSGTSQAFRTAASREIQHFVAELADYIMSKDNHSNRELAYIQSE
                     GLVTLVFTAGSHALDMNNQEREKLKQRVIMQLRMLIRGSTYYANKQASST"
     gene            complement(6567..6866)
                     /locus_tag="PXD81_001019"
     CDS             complement(6567..6866)
                     /locus_tag="PXD81_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4298 domain-containing protein"
                     /protein_id="HDL5537060.1"
                     /translation="MKKADIQKMQDCYNQWVELLPELEKGIEQWKRASALLEPLSRFY
                     ASPEWRELHDSFDEQLDTKGNYSILSEDALWNALSEHHQLALEWLKLSTAYITKE"
     gene            complement(6955..10770)
                     /locus_tag="PXD81_001020"
     CDS             complement(6955..10770)
                     /locus_tag="PXD81_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HDL5537061.1"
                     /translation="MKGSGLATVGAPETGDNGQTIYTVDVAKAGAPTVTRGNVADAIN
                     NSEKTSSVVAGSKAVTVKAGKEDDKGNTEYTVDVATDKSIGRDNDGNMTVNTDNVTIV
                     KDPTTGEVKANTTTLNNSPEGKVTEPTGDDAKKLVTAGDIANAINNSGFNVTAGDGVD
                     GGETKGKKTQLIKPSETVTFDAGKNMTLTQADGKFTYTTKDNVAFNSIDMSNGKPDTT
                     GSITNLKSGVGGTFADKAAPTDAERKAIADNINNATGDTLNNAVNVGDVQAAMKAATT
                     KVEGDKGVTVTSKTNDDGSTTYTVEAKTDGTTIKVNDKGEITANTSELTNNPDGKVEE
                     PTEPNALVTAKTVADAINNAGFNIKANGDEKSLVKTGDTVQFLNGKNIEITRDGNNIT
                     VSTAKNVNFDSVQFGNEGPKITNNGGNINVGDKDGNAVKVTNVAAGDVNADSKDAVNG
                     SQLYTFAMASREEVKSTDKSVTVNTTKNADGANVFDLSVNTDDVTIVKDPTTGAIKAN
                     TTALNDANNDGRIDEPTADDAKKLVTAGDITNAINNSGFTLKTSAVEGGEKLSGGDEL
                     INPGKAVEMVAGKNLTVKQEADGKVIYATKDDVKFSSVTSNTVTVPTDEADPANNPIT
                     INKDGINAGNKAISNVASNLIPVTADDKVQPADNNPTNLADKLSNAATLGDVLNAGWN
                     LQGNGKAVDTVVHNDTVDFINGKGTTVTVENKDGKNTIKVDSPIEFVNQDPTDSSTPS
                     NTAKFTGEAPVQLGNVASSVRNEDGSTPEGKDRAEAIKNAEGDKLNNVVNLGDLQAAT
                     NAATTKVGGNRGVTITPSTNADGSTTYNVEAKTDGTTIKVDNEGNITANTSELGNNED
                     GTVKAPTQPNALLIAQTVADAVNNAGFNIKSAGNKAAGDQAATKLVKTGEEVVFEAGD
                     NLTVKRDGNQFTFATAKDVSFNSVQFSENGPKITNDGDNIKVGDKDGKPTKITNVADG
                     DISPVSTDVINGKQLNNYAKVNGNNIGTDEDGSINIVNGNGTTITSDKAGEVKVNVNI
                     TDLTVADNGKINVQDPNGTGSRFVNATTVANAVNNVSWNVDSKAVGTGAVEGDKAPAK
                     VKAGSTVSVNAGNNIKVTRKGSDVTVAVSDTPEFTSKTGDTLVNNNGVTINNGSAGKA
                     VSLTKDGLNNGGNRITNVKAGEADTDAVNVGQLKGAVNHLNNKIHRNNREARAGIAGS
                     NAAAALPQVYIPGKSMVAAAGGTFKGENALAVGYSRSSDNGKLILKLQGNANSRGDFG
                     GGVGVGYQW"
     gene            complement(10733..11218)
                     /locus_tag="PXD81_001021"
     CDS             complement(10733..11218)
                     /locus_tag="PXD81_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5537062.1"
                     /translation="MDTGSITVDNTTGAVTTPAEEDKVATTKTVSEAIQKAGWNAKSG
                     GNKADGDQEAAELINPGEKVIFAAGDNLKVKRVGTIFTYETAKDVKFDSVTFGDNGPK
                     ITNKDGNVNIAGNDGNPTKITGVKAGEADTDAVNVSQLKQAAASQNRSERFRFSNCWC
                     T"
     gene            complement(11227..13386)
                     /locus_tag="PXD81_001022"
     CDS             complement(11227..13386)
                     /locus_tag="PXD81_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HDL5537063.1"
                     /translation="MGARATSANAIALGTDTAATGNRATAFGAGALATGNRSTVMGWR
                     SAASGTRSFAMGSGAMGDKSVANATYSLALGSSANANATSTATRGTAIGYNATVSGVN
                     ATALGVSAVSSNQSTVAIGDSAKASAGFAIALGSGANVALNNSVALGSNSTVSNAVAV
                     PSATVNGITYSGFAANTTAAGNVVSVGSNTIKRQIQNVAAGQISATSTDAINSSQLYM
                     AMNATGNLANSTKNILGGNATVKPDGSVTYTNIGGTNKNTIEEALKAVKTEVVAGSNV
                     NITNATGANGQTIYTVNAYNTTANSSSPDYITVTGKAATAANTTNYEIGLTKKAIDDF
                     TKDTQATVVSNDGTVTVKSTERNANGTVIYDLSVNIPAQASQIQYFSVNSTVPENQAN
                     DGAKSRNSIAIGPNATATGGEQAAVALGTNSNANGNGALSLGVATVSKGIQATAVGHS
                     ANATANGTTALGRQTNATAGDATAVGSNANATAEKASAFGVAANASANASLAVGANSI
                     ASAQSAVAVGTRANATAQFATALGMGAQATLNSSVALGSESVVRAATPTENATVGGIT
                     YNGFAGVNKDTNYVVSVGSAGKERQIQNVAAGQISATSTDAINGSQLYMAMNATSNLA
                     NSTANNFGGGSVVNPDGSVTQPKYNVTNAAGTQYGNTATNVGDAITNLNNYVNQGFNI
                     KDNAGETKGTVTPNESVQFVNGKGTVSNVTQEADGVK"
     gene            complement(13615..13815)
                     /locus_tag="PXD81_001023"
     CDS             complement(13615..13815)
                     /locus_tag="PXD81_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ESPR domain-containing protein"
                     /protein_id="HDL5537064.1"
                     /translation="MNQIFKVIWNQATQSWVAVSELQRAKGKTKSVSSASKATLALLA
                     GASAVLGANDAVAVVSYRFNNK"
BASE COUNT         3894 a         3199 c         2597 g         4276 t
ORIGIN      
        1 actaccttac actcttgagc tgtaatatac tatttaactg cggtaataac tgcacgatca
       61 gtctgagttg ttgtatcaac actaaggcga ggtcattttg cttttgctga ttttcttgct
      121 caaattgggt gaggtcttgg tcgatagcgg tcaatttttc cgataatttt gcaaatgaac
      181 ggctctctga ggcaatttgc ccgatgatgt ttgccacctc tctggcgtgt gagaagaaca
      241 ttgcggaaaa atccacttgt tgattaatct ctcggctagc aatacggtaa gcccctaagg
      301 tggaaatata acccagcaag gtgtaagcaa tacctaacaa ttttggggcg aaggttaaat
      361 cgttcgcata tttttgtggt tcgctgtaca tattggtaat cgcattactc agtgccgata
      421 aatggttatg cacatcacgg cgggcaatac ggtaaggcag ttgctcgtta taaccaaact
      481 gcaactgggc gataatatgg cgtaaatatt gcccactggc tttcaagccg ttatgtaagt
      541 tacgttgtaa atttaaatac ttccaatccg gccataagaa tgccactgcc agccacgcaa
      601 tcgctgtacc tagtaaggta tcaagcattc gcattaagat gccgctttca aggctcacgc
      661 ctgcaatatc aaggcttatc attaccaaca cagtgatata aaaggttgaa aagccataat
      721 gtaccaagcg gaaaaaggta taaagcgaac cggtcagtac gattagccca agctgggctt
      781 cgaggctgag cgccacgtta aacagttcaa aagagccacc aaccagcacg ccgaaaattg
      841 tgccgatcac acgctgaatc agtcgggtct tagtagcagt gtaattcggt tggcagacaa
      901 aaatggctgt gagtaaaatc cagtagcctt tgctatcaaa gcctaaaatc ggcacaatcg
      961 cactgcacaa cagcaccact atcgacaacc gaacggcgtg tcgaaacagt tgggattcaa
     1021 aggttaaatg ggctttaatt gcttggctga tattgcttaa gccggtaata ttttcggcga
     1081 tcaagcggtg agattttgtg aaatttttac ttaacggttc aatatgctca ccactgcttg
     1141 agccttgttc aatctgggct agttgccgct caatattgcc taaattttct gcaatagcac
     1201 gccagcgata agcacttttc aagccccgtt cttgatgata attcaatgaa ttaagcaagc
     1261 cctgcaaggc tttttcgcca cgaacgccgt gttcatatac ttctctatgg cgtaacgaca
     1321 ttgcaatttt ttgacacgcc gcagcctgta attccagcac ccgttgaaag cggaacatta
     1381 aatcactgtt acttaattgt tggaacagtt cgtgatattg ataatggctg gaacttgccc
     1441 gctctaaaat atcttgagcg gtgaaatagt aacgcaacat tcgctgtgtc cgttgctgct
     1501 tgtgctggcg gcttaggcgg tagaataggg caactctggt tttatcaaag gcagccatca
     1561 cctcactatt ggctttcgcc aaggctagtt gtttggcagc taaatcgtca tcatcagggt
     1621 caaaataggc agatttcgcc agtaaataat ggctcaacgc aaggtaggcg gacgctaaat
     1681 tctcctgcac ggttcgattc gggaaaatca ggtacaccaa aataccgacc aaaccataga
     1741 gtaaggtgcc aagtaagatc atcaccacat tgtgatacca caatacttcg ggcgtataag
     1801 ccaggctgtt ataaaccgcc acaatcagcg tgccaaaagc aatggtacta tagcgttgcc
     1861 cgattgcccc taacattacc agcaaaaagg tgcaaaccac cgccaccggc acaaacagcc
     1921 agcccaaatg gagcgacaca ctcgccccaa tcgaggaaag cgagaacgcc actaggctga
     1981 taatcagatt tttcgctcgt ccgattaggc tgttgtctaa atccaccaaa ccgccggcga
     2041 taatgcctaa catcaacggc atcgcaaaat cagaaatacc gagctgccaa atgctgaccg
     2101 ccgcaatgtt tagcgaaata aacatcggca gggttttaat tacattttcc gaaagccaac
     2161 tgtgttgtag atatcttaaa aatttattca tatttgctca tttcgggcag gcacaggacc
     2221 tgcccctacc tacactaccc caaacgttct gccaaatagc catcataatc cggcacattc
     2281 acgctgattg cttgattgaa caaatccgca tttaacagaa aattagcggt ggtttcgtta
     2341 atcgccaccg gaatattcca aacggtcgca atccgcatca gggctttcac atcaggatcg
     2401 tgcggagcgg cattcatcgg atcccaaaag aacaccagca gatcaatttt cttttctgcg
     2461 atcagcccgc ctaattgctg atcgcccccc atcgggccgc ttaacaatga agtaatttca
     2521 agccctgttt ctctggcgag caaatgcccg gtagtgccgg tggcatatag cttatgcgag
     2581 gcaagttggg cttgatgagt ttgtgtccaa cgaattaaat cttgtttaca gctatcgtga
     2641 gccactaagg caatctgttt ttgttttgcg atggttctga cggtggtttt catttttagc
     2701 tcctttgcaa aatttttcac aaatcatacc gcttattttt cgcttttgca ttgactaatc
     2761 acggcttatg gctaaaatca ggtaaaaatt cgcattttaa aacaaaggat taaccgcaat
     2821 gaacttatac aacatcaaac accctgaaga acaagtcaat ttcgcccaag cggtacgcca
     2881 aggcttaggc aaaaatcaag ggctattttt ccctgaagtg ttacctaaat tagatagcat
     2941 tgatgaatta ttggatttac ctttggttaa acgcagccaa aaaattcttt cagccttaat
     3001 cggcgaagag ttaccgcaag aaaccttaaa tgcaatggta aaaaatgcgt tcactttccc
     3061 ggcaccgctt gccaaagtca atgatgatat ttatgcgtta gaattatttc acggtccgac
     3121 tttagccttc aaagattttg gtgggcgctt tatggctcaa gccttagcca atgtacgagg
     3181 agacggcaaa attactattt taaccgctac ctcaggtgac accggtgcgg cggtggctca
     3241 tgctttctat ggtttggaaa atatcaacgt ggtcattctt tacccgaaag gcaaaatcag
     3301 cccattacaa gaaaaattat tctgtacctt aggcggtaat attcgcactg ttgcgattga
     3361 atccgacttt gatgcttgcc aagccttagt gaaacaagcc tttgatgatg ccgagctacg
     3421 ccaagcgatt ggtttaaatt ccgcaaattc catcaacatt agccgtttac tggctcaaat
     3481 ttgctactac tttgaagcag ttgctcagtt accaaaagaa aaacgagata acgtagtggt
     3541 ttcagtacca agcggtaatt tcggtaactt aaccgccggc ttaattgcca aaacattagg
     3601 tttaccgatt aaacgtttta ttgcctcaac caatgccaat gacaccgtgc cacgctattt
     3661 acaatcaggc aaatgggaac caaacgcaac cgttgcaacc ctttctaatg caatggacgt
     3721 aagccgcccg aacaactggc cacgagttga agaattattt aaacgcaatg gctggagttt
     3781 gtctgaattg ggttctgccg cattaaacga tgccgaaacc gaagcggctt tgaaagctca
     3841 atatgcagaa ggttatctgt gtgagcctca cggtgctatt gcttaccaaa gcttgaaaga
     3901 tcagctacaa caaggcgaaa ccggtatttt cttatgcacc gctcacccgg caaaattcaa
     3961 agagtcggtg gaacacattt tagcgattga attgccgtta ccggaagcat tagataagca
     4021 caacaaactg ccactgttat ccgatgaaat ggcagcagat tttgcaacat tgcgtgcata
     4081 tttgttgaaa taaatattca ataatagaaa agggattaag tattaaactt aatccctctt
     4141 tttatgcatt acaagcggtt atatttgcct attattttgc aaaataagca gctaactcat
     4201 cactaccgcc aatatattta ccgccgataa atacttgtgg cacagaggtt ttacctgtaa
     4261 ttgctcgtac agaaattgtg cttgcatcac gacctaacac aatctcctca aaagtatagc
     4321 cttgctcttt taataatgct tttgcttttg cacagaacag gcaacccggt ttagtgataa
     4381 tcgaaacaga ctctttagct gtccaatttg gacttaaata tttaatcatt gtatcggcat
     4441 ctgatacttt aaacggatcg cccggttctt gtggctcaat aaacattttt tccacaatac
     4501 cgtttttcac cagcatcgaa taacgccaag aacgtttacc aaacccaagg tcatttttat
     4561 caactaatag ccccatacct tctgtgaact caccattacc atcaggaata acagtaatat
     4621 tttcagattc ttgatctgct ttccaagcgt tcattacaaa ggtatcatta actgacatac
     4681 agatgatatc atctacccct aatgccttaa attcacaagc taattcatta tagcgaggca
     4741 aatgtgttga tgaacaagtc ggtgtaaatg ctccgggtaa tgaaaacaca accaccgttt
     4801 tgttattaaa taactcatcg gttgtgacat caacccaagc atcgttttga cgtgtacgaa
     4861 atgttacact gggtactttc tgacccgtca tatctttaaa agacatatta atgttctcct
     4921 atagaattat atattacaaa aatcttaggc attatactca caaatatggc ataattcagc
     4981 ctatcagaac aataggaaaa atttaaattg gaggaatcaa gtgaatattc gagatttaga
     5041 ataccttatt gcacttgcag aacataagca tttccgtcgt gcagcagaat cttgtaatgt
     5101 tagccaacct actttaagcg ggcaaattcg caaattagaa ggcgaactag gcactattct
     5161 gcttgaaaga accagtcgca aagtcctctt tacgcaagca ggcttaacat tagttgaaca
     5221 agccaaggta gttttacgtg aagtaaaaat tctaaaagaa atggcaagca accaaggtaa
     5281 agagatgtct ggcccgcttc atgttggtgt aatcccaacc ttggggcctt acatttcgcc
     5341 aattattgtg cctgcattaa aagcagaatt tgcagattta gagctgtata tttacgaatt
     5401 acaaaccaac caattattag atcaacttga atccggccag cttgattgtg gcattatctc
     5461 atttgtaaaa gagagtgaat cttttattga agtgcctatc tttaatgaag aaatgtactt
     5521 ggtagttcca gaacaacacg aatgggcagc gcttaaaaaa ttcgatatga atttattaaa
     5581 aaaccgtgag ttgctctttt tagacagtgg ccattgttta cgcactaata cgctagacta
     5641 ttgtctatcc attggagcaa aagaaaatcc acactttaaa gctaccaacc ttgaaacctt
     5701 acgcaatatg gtagcagcaa atgttggcct agcattgatt cctaaattag ccgctaaacc
     5761 gattaaagga gtacattata ttccacttga aaacccgaaa ccgtttcgag ctgtgggaat
     5821 ggtgtatcga ccaggttcac cattacgcgc ccgctatgag cgtttagctc aagccattac
     5881 taaaattatg caatccgagg cgtaaaaatg gcaaacggta tgagtgtgcg cgcaattcag
     5941 aaagaaaaaa cacgcagagc attagtagaa gccgccttta atcaattaag ctcagaaaaa
     6001 aatttctcta atctcagttt gcgtgaagtt gcacgtgaag ctggagtcgc accgacttcg
     6061 ttttaccgcc actttaaaga tatggacgaa ctaggcttag caatggtgga cgaatccggg
     6121 ctactattac gccaattaat gaggcaagct cgaaaacgaa ttgcaaatgg cggaagtgtt
     6181 gtcgtggttt cagtggaaac attcttcgaa ctgattgccg accgccctaa cgttttccag
     6241 ctattactac gcgaaagctc cggtacatcg caagcgttta gaaccgcagc atcacgcgaa
     6301 attcaacatt ttgtagctga attggcggac tatattatga gcaaagataa tcacagtaac
     6361 cgagaacttg cttatattca atcggaaggt ttagtcacgc tagtatttac tgccggttct
     6421 catgctttgg atatgaacaa tcaagaacgg gaaaaactaa agcaacgtgt cattatgcag
     6481 ctaagaatgc tgattcgtgg ctccacctat tatgccaata aacaagcttc atcaacataa
     6541 aaaaacagac gcctaagcgt ctgtttttat tctttcgtaa tatatgcagt cgatagcttt
     6601 aaccattcta gtgctaattg atgatgttca gataacgcat tccataacgc atcttctgac
     6661 aagatactat aattgccttt agtatctaac tgttcatcaa aactatcatg taattcacgc
     6721 cattccgggc tagcataaaa ccgacttaag ggttccaaaa gtgcagaagc tcgtttccat
     6781 tgctcaatcc ccttttccaa ttctggtaat agctctaccc attggttata acaatcctgc
     6841 attttctgaa tatctgcttt tttcattctt taccgcttac accataaatt ttagcctcaa
     6901 tataacataa aaatcccctt ccgaagaagg ggatttcaac tcaattaggc taaattacca
     6961 ctggtatcca acgcctacac cgccaccaaa gtcgcctcgg ctattcgcat taccttgtaa
     7021 tttcaagata agcttaccgt tatcacttga acgtgaataa cctactgcaa gcgcgttttc
     7081 acctttaaat gtaccacctg ctgcagccac catagattta cctggaatgt aaacctgcgg
     7141 taacgctgct gctgcatttg aaccggcaat accagcacgg gcttcacggt tattacgatg
     7201 gatcttgtta tttaagtgat taacagcacc ttttaactga ccaacattaa cagcatcggt
     7261 atctgcctca cctgctttaa cattagtaat acggttacca ccattattta agccatcttt
     7321 cgttaagctt accgcttttc ctgcagagcc attattaatg gttacgccat tattgttcac
     7381 caatgtatca ccagtcttgc tagtaaattc aggggtatct gaaacggcta cagttacatc
     7441 agaaccttta cgtgtcactt tgatattatt acctgcattc acgctcactg tactacctgc
     7501 ttttactttc gcaggggctt tatccccttc aaccgcacct gtaccaactg ctttagaatc
     7561 tacgttccag cttacgttgt ttacggcatt tgctactgtt gttgcattca caaagcgtga
     7621 accggtaccg ttaggatctt gaacattgat tttgccgtta tcagcaacgg ttaaatctgt
     7681 gatgtttacg ttcactttca cctcaccagc cttatcactg gtaatagttg taccattacc
     7741 atttacgata ttgatagaac catcttcatc agtaccaatg ttattaccat tcactttcgc
     7801 atagttgttc agctgtttac cgttgataac atctgtgctt actgggctaa tatcgccatc
     7861 tgctacatta gtgatcttag ttggtttacc gtctttatca ccgactttga tgttatcacc
     7921 atcattagtg attttcggac cattctcact gaactgtacg ctattgaagc tcacatcttt
     7981 tgctgtcgca aaagtaaatt gattaccatc acgtttaaca gttaagttat cacctgcttc
     8041 aaacacaact tcttcgcccg ttttcactag cttagtcgca gcttggtcac cggcagcctt
     8101 attacctgca gatttaatat taaagcctgc attattcact gcatctgcaa cagtttgagc
     8161 aatcaataac gcatttggct gagttggtgc tttcaccgta ccatcttcgt tgttacctaa
     8221 ctcactggta tttgcagtga tgttaccttc attatctact ttaatagtag taccgtcagt
     8281 tttcgcttca acgttataag tagttgaacc atcagcattc gtgctaggtg tgatagttac
     8341 acctcggtta ccgcccactt tcgtagtagc tgcattagtt gccgcttgta aatcacctaa
     8401 gttcactacg ttgtttagct tgtcgccttc agcattttta atcgcttctg cacggtcttt
     8461 accctctggg gttgagccat cttcgttacg aacactactt gccacattgc ctaattgaac
     8521 aggggcttca cctgtgaatt tagccgtatt gcttggcgtt gagctatcag taggatcttg
     8581 gtttacaaac tcaatcgggc tatctacttt aatcgtattc ttaccgtctt tgttttcaac
     8641 cgtgaccgtt gtacctttac cgttaataaa atcaaccgtg tcattatgta ccacagtatc
     8701 aaccgctttg ccgttacctt gtaaattcca gcctgcattt aatacatcac ctaatgttgc
     8761 tgcattactt aacttatcag ctaagttggt tggattattg tctgctggtt gaaccttatc
     8821 atctgcagtc acaggtatca gattagacgc aacattgcta atcgctttat tacctgcatt
     8881 gataccatct ttattgatag taattggatt atttgcagga tctgcttcat cagttggtac
     8941 agttacagtg ttagaggtta cgctgctaaa cttaacatca tctttagtcg cataaatgac
     9001 tttaccatca gcttcttgct tcacagttaa gtttttacct gcaaccattt caactgcttt
     9061 acccggatta atcagttcat caccacctga aagcttctct ccaccttcta cagccgacgt
     9121 tttcaacgta aagcctgagt tattgatggc gttggtaata tcacctgctg tcactaattt
     9181 cttagcatca tcagcggttg gttcatcaat tctaccgtca ttatttgcat catttaatgc
     9241 cgtagtgtta gccttgatcg caccggttgt aggatctttt acaattgtta catcgtccgt
     9301 attcacagaa agatcgaaca cattcgcgcc atcagcattt tttgtcgtgt taaccgtaac
     9361 tgatttatca gtcgacttca cttcttcacg agatgccata gcgaaagtat aaagctgact
     9421 accatttact gcatctttac tatctgcatt tacgtcaccg gcagctacat tagttacttt
     9481 gactgcatta ccgtctttgt caccaacatt gatattacca ccattgttag taatcttagg
     9541 accttcattt ccgaactgta cactatcaaa attcacattt tttgcagttg aaacagtaat
     9601 gttattccca tcacgggtaa tctcaatgtt tttaccattt aagaactgaa ccgtatcacc
     9661 tgttttcacc aggctcttct cgtcaccgtt tgctttaatg ttaaaacctg cattgttaat
     9721 tgcatctgca acggttttag ctgttactaa tgcatttggt tctgtaggct cttccacttt
     9781 accatcaggg ttatttgtta actcgctggt gtttgccgtg atttcgccct tatcgtttac
     9841 cttaatggtt gtaccatccg tttttgcttc aacggtataa gtggttgagc cgtcatcgtt
     9901 agttttagat gttacagtaa cacctttatc accttccact ttggtggttg ctgctttcat
     9961 tgctgcttgt acatcaccca cattcacagc attattcaac gtatcacctg ttgcgttgtt
    10021 aatattatcc gcaatcgctt tacgctctgc atctgttggt gctgctttat ctgcaaatgt
    10081 accacccacg ccgctcttca gattagtgat tgagcctgtt gtatctggtt taccattgct
    10141 catatcaatg ctgttgaatg caacattatc tttcgttgtg taagtgaatt taccgtcagc
    10201 ttgggtaagt gtcatatttt tacccgcatc aaaggtaact gtttcacttg gcttgattag
    10261 ctgtgttttc ttacctttgg tttcaccacc atcaacacca tcacctgctg ttacattaaa
    10321 gcctgaatta ttgattgcat tagcaatatc acctgccgtc actaatttct tagcatcgtc
    10381 acctgtaggc tcagtaactt taccttcagg agaattattt aatgttgtgg tattcgcttt
    10441 aacttcgcct gttgtcggat ctttcacgat agtaacgttg tcagtattca ctgtcatatt
    10501 accgtcatta tcacgaccga tagatttatc cgttgcaaca tctaccgtat attcagtatt
    10561 gcctttatca tcttcctttc cggctttaac agttacggct ttagaaccag ctactacaga
    10621 tgaggttttc tctgaattat taattgcatc agccacatta ccacgagtta ctgtaggagc
    10681 acctgctttc gctacatcta ccgtatagat tgtttgacca ttatcacctg tttcaggtgc
    10741 accaacagtt gctaaacctg aacctttcac ttcggttttg tgacgctgct gcttgtttta
    10801 actgacttac attcaccgca tcagtatcag cttcacctgc tttcacgcct gtaattttgg
    10861 ttggattacc atcattacct gcaatattaa cattaccatc tttattcgtg attttcgggc
    10921 cattatcacc aaatgttacg ctatcaaatt tcacatcttt cgctgtttcg tacgtgaata
    10981 tagtgccaac acgtttaact tttaagttat cgcctgctgc aaaaatcact ttttcgcccg
    11041 gattaatcaa ctctgctgct tcttgatcac catcagcttt attgccacca gattttgcat
    11101 tccaacctgc tttttgaatc gcttcagaaa ctgtttttgt agttgcaact ttatcttctt
    11161 ctgcaggtgt agttactgca cctgttgtgt tatcaaccgt gatagaaccg gtatccacat
    11221 caaaggttac tttacgccat cagcttcttg tgtaacattt gaaactgtac ctttaccgtt
    11281 tacaaattgt acagattcat ttggggtcac tgtgcctttg gtttcacccg cattatcttt
    11341 aatattgaaa ccttggttta catagttatt taagttcgta attgcatcgc caacgtttgt
    11401 tgcagtatta ccatactgag ttccagctgc attcgttaca ttatatttag gctgagtcac
    11461 agagccatcc ggattcacaa cagaaccgcc accaaaatta tttgcagttg aatttgcaag
    11521 gttactggtt gcattcattg ccatataaag ttgtgaacca ttaatcgcat cggttgatgt
    11581 agcagaaatc tgacccgctg ccacattttg gatttgacgc tctttacctg cagagcctac
    11641 agaaacaaca tagttagtat ctttatttac cccggcaaag ccattataag taataccccc
    11701 taccgttgca ttttcagttg gtgttgctgc ccttacgact gattcgctac ccaaagcaac
    11761 agaactattc agagtagctt gagctcccat acctaatgct gtagcaaatt gagctgttgc
    11821 attagcacgt gtaccaacag ccactgctga ttgtgctgag gcaattgaat ttgcacctac
    11881 ggctaaagag gcatttgcac tcgcatttgc tgcaacacca aaggcagaag ctttttcagc
    11941 cgttgcatta gcattagaac ctaccgctgt agcatcacct gctgtcgcat ttgtttggcg
    12001 accaagtgcg gttgtaccat tagccgttgc atttgctgaa tgaccaactg ccgtagcttg
    12061 aatacctttt gaaaccgtag caacacctaa agataacgct ccgttaccat ttgcatttga
    12121 gtttgtgcct aatgctactg ccgcttgctc tccacctgtt gccgttgcat tcgggccgat
    12181 cgcaattgag tttctgctct tcgcgccatc attagcttga ttctcaggta cggttgaatt
    12241 cacagagaaa tattgaattt ggcttgcttg cgcaggaata ttgacagaaa gatcataaat
    12301 aaccgtgcca tttgcatttc tctctgtact ttttacagtt actgtaccat cgtttgaaac
    12361 aacagtagct tgggtatctt tcgtaaagtc atcaatggct ttcttagtta aaccaatttc
    12421 atagttagtt gtattcgcag cagttgcagc tttacctgtt acagtaatgt aatcagggct
    12481 agaagaattt gccgtggtgt tatacgcatt tactgtataa atagtttgcc catttgcacc
    12541 ggttgcattt gtaatattaa cattgctacc tgcaacaact tctgttttta ccgcttttaa
    12601 tgcttcttca atcgtatttt tatttgttcc accaatatta gtataggtaa cagaaccatc
    12661 aggctttact gtagcattac cacctaaaat atttttagtt gaattagcaa ggttacctgt
    12721 tgcgttcatt gccatataaa gctgtgaact attaattgca tcggttgatg tagcagaaat
    12781 ctgacccgct gccacatttt ggatttggcg tttgatcgtg tttgagccaa cagaaacaac
    12841 attccctgct gccgtagtat tagccgcaaa gccggaataa gtaataccat ttaccgtcgc
    12901 agaaggaact gccacagcgt tggaaacggt tgaattactc cctaaagcaa ccgaattatt
    12961 tagcgctaca ttagcacctg agcctaaggc aatagcaaaa ccggcacttg ctttagcact
    13021 atcaccaatt gcaaccgtag attggttgga agatactgca cttaccccta aagctgtcgc
    13081 attaacacct gaaactgtcg cattataacc aatcgctgta cctctagttg cagtagaagt
    13141 tgcattagca ttagcacttg aacctaaagc taaagaataa gtagcatttg caacagactt
    13201 atcccccatt gcacctgaac ccattgcaaa tgaacgagtg ccactagcgg cagatctcca
    13261 tcccattact gttgaacggt taccagtagc tagagctcct gcaccaaaag ctgttgcacg
    13321 gttacctgtt gcagcagtat ccgtacctaa tgcaatagca tttgctgaag tcgctctagc
    13381 ccccatacca atagcaatag aaggaactaa tgcccaattc tgaccagtgt tattgagact
    13441 ctctacacca atattatagt aatgtaagaa gtatcttctg gatccactaa atttgttcta
    13501 tgcgttgcat tcgtatctgt accaattgca atagtagatc tgccagttga gattgaatcc
    13561 gcaccaatag caattgcgtt ctccgatgat gcagttgctt gtgttgaaat ataattactt
    13621 gttgttgaac ctataagata caacagccac agcatcatta gctcccaaca cggcactagc
    13681 tccagccaat aaagccaatg ttgccttact tgcagaacta acagatttcg ttttaccttt
    13741 cgctcgttgt aattcagata ctgcaaccca actttgtgtt gcctgattcc aaataacttt
    13801 aaaaatttga ttcataatcg accctatata aaaataaaaa agattacgta gaacctatgt
    13861 tcaacataat cttcaccaat ataaaaaacc gccacctatt taagtgaaat aacttactta
    13921 tagactcaga aatttaagtg cgcgtatttt acgctatttg taatat
//
LOCUS       DANLYI010000048        13349 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.048, whole genome shotgun sequence.
ACCESSION   DANLYI010000048 DANLYI010000000
VERSION     DANLYI010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 13349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13349
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.048"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            347..991
                     /gene="rppH"
                     /locus_tag="PXD81_001024"
     CDS             347..991
                     /gene="rppH"
                     /locus_tag="PXD81_001024"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011848673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HDL5537065.1"
                     /translation="MIDFDGYRPNVGIVICNKHGQVLWAKRFGQNSWQFPQGGINEGE
                     NIEAAMYRELYEEVGLSKKDVRLLWASKYWLKYKLPKRLVRNEGSGPVCIGQKQRWFL
                     LQLIGDESLINLKTTKNPEFDGWRWVSFWYPVRQVVSFKRDVYRKAMKEFAQVLQNNI
                     QKATDDKTGEKPRIERGETSPHTYKKTSQDSAKAYKREARKPYRPWHQHNQKGK"
     gene            993..1781
                     /locus_tag="PXD81_001025"
     CDS             993..1781
                     /locus_tag="PXD81_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HDL5537066.1"
                     /translation="MTILLSCLFLGVFVGFLAGLFGIGGGLIIVPSLVFLLPMAGVPP
                     EYIMSTALGTSFSTIIITAFASAQRHHKLGNVDIQTSKYFIPALMVSVFLSGLVVSRL
                     DPQLMSRIFAVMVLYLAGKMIFSIKRAPKNKPLTVQTTIIGGSIIGALASMAGIAGGA
                     FIVPFLNDRGLEMKRAIGTSSFCGAFLGLSGTISFIVSGWGLEGMPDYSLGYVYLPAL
                     FGITATSFFTSKMGASAANALPVPMLKKAFAVLLVCIAINMFLK"
     gene            1789..2586
                     /gene="lgt"
                     /locus_tag="PXD81_001026"
     CDS             1789..2586
                     /gene="lgt"
                     /locus_tag="PXD81_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HDL5537067.1"
                     /translation="MNEQFIQFPQISPIVFEIGPIALRWYGLMYLAGFAFAYWLGMRR
                     AKNSNGVWTTEQVDSLVYTCFWGVVLGGRIGDVFFYSFDRFLQDPLYLFRIWEGGMSF
                     HGGLIGVIVAMIWVSYRQKRNFWQTADFIAPLVPFGLGMGRIGNFINDELWGRVTDVP
                     WAVLFPSGGYLPRHPSQLYEAFLEGVVLLFILNWFIQKPRPAGAVSGLFLIGYGTFRF
                     IVEYFREIDHNVNTTADLITRGQLLSLPMIIGGILIMAWAYKVNKVK"
     gene            2673..3341
                     /locus_tag="PXD81_001027"
     CDS             2673..3341
                     /locus_tag="PXD81_001027"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HDL5537068.1"
                     /translation="MSKKYKVIGFDLDGTLINTLPDLTLVANSMFLEAGLPTVTQEKV
                     LTWIGKGADIFFQNAVAHTGRIFQADELVKMRVSFDKYYATYVCEESTLFPNVKETLE
                     ILKANDYPLVVITNKPTKLVEPVLSAFGIYHLFDETLGGQSLPKIKPHPDPMFFICEK
                     FGITPSELLFVGDSENDVIAAKAAGCDVVGLTYGYNYNVPIEQSNPTFVTSEFKDVLK
                     IVGL"
     gene            3411..3671
                     /locus_tag="PXD81_001028"
     CDS             3411..3671
                     /locus_tag="PXD81_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HDL5537069.1"
                     /translation="MPTIALRQVGGSVAMVIPPAFLEALKLSVGSKVDLSLNDNQLVI
                     NPARPKVKYSLEELLRQSDFEAARNEESQSWLSEPAIGKEIL"
     gene            3668..4000
                     /locus_tag="PXD81_001029"
     CDS             3668..4000
                     /locus_tag="PXD81_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HDL5537070.1"
                     /translation="MKRGDIYWLDLDPTLGHEQSGFRPVVIVSATAFNLASKLPVVVP
                     ITNGGAFAERLGFAVSLKDAGLKTTGIIRCDQPRTVDIAQRHGKFIEALPESLLDEIM
                     ARVSVIFE"
     gene            4127..5158
                     /gene="trpS"
                     /locus_tag="PXD81_001030"
     CDS             4127..5158
                     /gene="trpS"
                     /locus_tag="PXD81_001030"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HDL5537071.1"
                     /translation="MSKPIVFSGVQPSGELTIGNYLGALRNWVKMQDDYDCVYCIVDQ
                     HAITVRQDPESLRKSTLDVLALYLACGIDPNKSTIFIQSHVPEHAQLAWVLNCYTYFG
                     EMGRMTQFKDKSARYEENVNVGLFTYPVLMAADILLYQANQVPVGDDQKQHLEITRDI
                     ANRFNALYGKKDSEGNLIEPVFAVPEVFIAKSGARVMSLLEPTKKMSKSDDNRNNVIG
                     LLEDPKAVAKKIKRAMTDGDEPPVVRYDVQNKAGVSNLLDILSAITGKTIAELETEFE
                     GKMYGHLKTEVADKVSELLTNLQERYHHYRQDEALLEKIYREGAEKARAQAKATLDKV
                     YELVGFVRY"
     gene            complement(5222..5977)
                     /locus_tag="PXD81_001031"
     CDS             complement(5222..5977)
                     /locus_tag="PXD81_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HDL5537072.1"
                     /translation="MKFIKKLSYSVFLATVITACATTESINQEAAQSYAQVKTQARQQ
                     NAIDNSSATAKRIHTVFHKMKPYAERANQTGVPFHWEITVLKSDELNAWAMPGGKMAF
                     YTGLVERLNLSNDEIAVVMGHEMAHALKEHGKSDRTVSAITSIVGAIADVAVTASTGV
                     STEGLLSTGVDLIATKPFSRSQETEADEVGLILMAESGYNPSAAPNVWVKMSKANGDS
                     GLSIFSTHPSNADRQENLARLVPEAMKIYNARK"
     gene            complement(6033..6560)
                     /gene="ppa"
                     /locus_tag="PXD81_001032"
     CDS             complement(6033..6560)
                     /gene="ppa"
                     /locus_tag="PXD81_001032"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010944216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HDL5537073.1"
                     /translation="MGLELVPAGKELPDDIYVVIEIPANSDPIKYEVDKETGALFVDR
                     FMATAMFYPANYGYVNNTLSSDGDPVDVLVPTPYPLQPGSVIRCRPVGVLKMTDEAGG
                     DAKVVAVPHTKLSKEYDHIKDVGDLPALLKAQIQHFFESYKALEAGKWVKVEGWGDVN
                     EARQEILESFERAKK"
     gene            6777..6852
                     /locus_tag="PXD81_001033"
     tRNA            6777..6852
                     /locus_tag="PXD81_001033"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6810..6812,aa:Lys,seq:ttt)
     gene            6875..6950
                     /locus_tag="PXD81_001034"
     tRNA            6875..6950
                     /locus_tag="PXD81_001034"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6908..6910,aa:Lys,seq:ttt)
     gene            6972..7047
                     /locus_tag="PXD81_001035"
     tRNA            6972..7047
                     /locus_tag="PXD81_001035"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7005..7007,aa:Lys,seq:ttt)
     gene            7140..8174
                     /gene="mltG"
                     /locus_tag="PXD81_001036"
     CDS             7140..8174
                     /gene="mltG"
                     /locus_tag="PXD81_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005613374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HDL5537074.1"
                     /translation="MLKKILIALGLAGVIACGGLFYGYQKLTSLAEHPITVQPNQLFV
                     LEKGVSSQKLAALLEEQGIVTHDDADLIPYLMRLYPELSKFKAGAYSLAGLTTVKDLL
                     AHLSSGKEVQLNVQFIEGKTFKIWREQLAKADYLQHTLQGKSEKEIADLLGIPHEKIE
                     GWIAPDTYNYTPNSTDLALLKRAYQKQKTALEQAWKNRAENLPLATPYEMLILASIVE
                     KETGIATERPQVASVFINRLRNNMRLQTDPTVIYGMGDRYNGNIRRKDLEEATPYNTY
                     QIDGLPPTPIAMPSEASIKAVANPDNTPYLYFVADGTGGHKFSKTLNEHNKAVAEWIQ
                     IERKRKNEQK"
     gene            8185..8805
                     /locus_tag="PXD81_001037"
     CDS             8185..8805
                     /locus_tag="PXD81_001037"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HDL5537075.1"
                     /translation="MRGKFIVIEGLEGAGKTTAHQVILKELEQAGINDVVMTREPGGT
                     PLAEKLRHLIKHETEEAINDKAELLMLYAARVQLVENVIKPALAEGKWVLGDRHDMSS
                     QAYQGGGRKLDRHLLETLKESVLGTFEPDLTIYLDLDPVIGLERARGRGELDRIEQQS
                     LDFFYRTRERYLELTQHNDKAVIINAEQPIEQVTADIQQVIRNWLK"
     gene            8805..9782
                     /locus_tag="PXD81_001038"
     CDS             8805..9782
                     /locus_tag="PXD81_001038"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HDL5537076.1"
                     /translation="MYPWLESTYQQLTDSFLQGRGHHALLFKTDVGLGTDTLIYKVAE
                     WLLCSQKLRHQPCLECKSCLLMQSNNHPDFHILEPIEDKDIGIDQVRELIYKLQNFAQ
                     QGGNTVVYIRGVDRLTEASSNALLKTLEEPRENVYFLLEAPLQATVLATIQSRSQTWV
                     LYAPQASEVMAWLQTQFPAISTDDLEIALRLCHSRPLICKNFIENDRLQQRKTFLQTF
                     WRFYKSHDVFLLFQAFAQEKELILQQLEWLESFFSDALKAKMAISHGWLNPDLQNGIL
                     PFSQQLTTHGLLKGHQILQQTQRDLTEINAVNPELMLLDCLTKLVLVFE"
     gene            9913..10155
                     /locus_tag="PXD81_001039"
     CDS             9913..10155
                     /locus_tag="PXD81_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HDL5537077.1"
                     /translation="MARTTSVTLGEPLNDFVNEMVASGRYGSTSEVVRTALRMLEEKE
                     QYLAQLRQAIDDGDASGYATETIDEMMLRVKAELNV"
     gene            10148..10432
                     /locus_tag="PXD81_001040"
     CDS             10148..10432
                     /locus_tag="PXD81_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDL5537078.1"
                     /translation="MYKLSAQALRDQEKLFRYSISQFGVQQTESYFADLKHILNLLAD
                     FPTMGTSAEYIRGGLRMHTHQSHTIYYRIKADHIFIVRILHNQMNHKRYI"
     gene            10437..11225
                     /locus_tag="PXD81_001041"
     CDS             10437..11225
                     /locus_tag="PXD81_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014992335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchF/TatD family DNA exonuclease"
                     /protein_id="HDL5537079.1"
                     /translation="MKDLFIIDSHCHLDSLDYETRHKNVDEVIENAKARGVHHFISVC
                     TTVGRFEAMKNLTAHRNDVSLSCGVHPLNVEDEPFDYDKLFQFAQDPKVVAIGETGLD
                     YHYTPETKALQQSLFVQQIEIANKLNKPLIIHSRSARQDTMDMLEQNNAEKCGGVLHC
                     FTEDWAMAKRALDIGFYISISGIITFRNAEELREVVRKVPLDRLLVETDSPYLAPIPY
                     RGKPNQPAYVRETCEYVATLKGVSTEELARITTENVQHLFNIKL"
     gene            11235..11756
                     /locus_tag="PXD81_001042"
     CDS             11235..11756
                     /locus_tag="PXD81_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1523 family protein"
                     /protein_id="HDL5537080.1"
                     /translation="MRAILKYFLILVSLSFFIVLGGAVNYAMPSYEDTVVTGMEVRRM
                     DKDGIISKANPADGEVRDVYFLFTEDPETKKVMVYRNEDTGWGLPPYFKFGSADIQAK
                     AQAYANEKQRVQIKYYGWRINWLNEFRNIVSIKPLEEAETVSKPIMSYVLYAVLAFLF
                     FLSVQFIRGIFKD"
     gene            11831..12532
                     /gene="lpxH"
                     /locus_tag="PXD81_001043"
     CDS             11831..12532
                     /gene="lpxH"
                     /locus_tag="PXD81_001043"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HDL5537081.1"
                     /translation="MTYYFIADLHLNENQPEITHLFLQFMQEKALLAEAVYILGDLFD
                     FWIGDDEQSELISTVKQAIKTLTTSGTKCYFICGNRDFLIGKRFSEQTGMVILPDYHV
                     IDLFGTSTLLCHGDTLCTDDVKYQQFRKKVHQKWRQAIFLMFPLSWRIRIAEKIRSKS
                     KQEKRDKSAEIMDVNAEVTAQIVAKYNTTQLIHGHTHRQAIHQHNGFTRIVLGDWKEN
                     YASILSVTEQGAEFI"
     gene            12529..13319
                     /locus_tag="PXD81_001044"
                     /pseudo
     CDS             12529..13319
                     /locus_tag="PXD81_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005618283.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
BASE COUNT         3963 a         2842 c         2779 g         3765 t
ORIGIN      
        1 ctatcacatc tttatccaca aatggtgggg ataactctgc tttatttgcg aagcaaaaat
       61 tgccctgctt attcatttta aaatttaaac ttattttgac gagaaaaaac gcaacaactt
      121 gatctttatc cagatcattg cttaagaaaa agatcgcatt tttagatctg attaattgct
      181 ctgttttaca acgttccgcc tgtttatcct caacttatac cacttatatt caatagttat
      241 ccacaaagtt attcacaagc ggttatattt tatggttttt ttgcaaatca acccaaagta
      301 tgaaacaatt ctcccattct agaattttta tcaattaggt ggtcgagtga tcgatttcga
      361 tggctaccgc ccaaatgttg gaatcgttat ctgtaataag cacgggcaag ttctgtgggc
      421 aaaacggttt ggtcaaaact cttggcaatt tccgcaaggt ggaattaatg agggggaaaa
      481 cattgaagca gcaatgtacc gtgagcttta tgaagaggtc ggcttaagta aaaaagatgt
      541 acgattgctt tgggcgtcta aatattggct aaaatataaa ctgccaaaac gcttggtacg
      601 taatgaaggc tctggtcctg tctgtatcgg gcaaaaacag cgttggtttc tactgcaatt
      661 aatcggtgat gaaagcctga ttaatttgaa aaccacgaaa aatccggagt ttgacggctg
      721 gcgttgggtc agcttttggt atccagtccg ccaagtggtc tcttttaaac gagatgttta
      781 tcgcaaagcg atgaaagagt ttgcacaggt tttgcaaaac aatattcaaa aagcgactga
      841 cgataaaacc ggtgagaaac cacgcattga gcgtggcgag acttcgcccc atacttataa
      901 aaaaacctct caggattctg ccaaagccta taaacgagaa gctcgtaagc cttatcgtcc
      961 ttggcatcaa cataatcaaa aaggaaaata agatgactat tttattaagc tgcctttttt
     1021 taggggtgtt tgtcggcttt ttagccggct tgttcggcat tggtggtggg ttaatcatcg
     1081 tgccgagttt ggtcttccta ctgcccatgg caggtgtacc gccggaatat ataatgtcca
     1141 ccgcactcgg cacatcattt tcaaccatta ttattaccgc ctttgcatcg gcacaacgcc
     1201 atcataagtt aggcaatgtc gatatccaaa ccagtaaata ttttattcca gcgttgatgg
     1261 tttcggtttt tctatcgggc ttggtggtaa gccgcctcga tccgcaactg atgtctagaa
     1321 tttttgcggt aatggtactt taccttgcag gtaaaatgat tttttcaata aaaagagcac
     1381 cgaaaaataa accacttacc gttcaaacga cgataatcgg aggttcaatc atcggtgcgt
     1441 tagccagtat ggcagggatc gcaggtggag cctttattgt accattttta aacgatcgag
     1501 gcttagaaat gaaacgtgcc atcggcactt cctcattttg cggagcattt ttaggtttat
     1561 ctggcacaat cagctttatt gtaagtggct ggggcttaga aggaatgcct gattattctc
     1621 tcggttatgt ttatttaccg gctctgtttg gtattacggc aacttcattt ttcacctcaa
     1681 aaatgggggc aagtgccgcc aacgctctac ctgtgccgat gctgaaaaaa gcctttgcgg
     1741 tgcttttggt ctgtatcgcc atcaatatgt ttttaaaata aggacactat gaacgaacaa
     1801 tttatccaat ttccacaaat tagcccgatt gtgtttgaaa tcggtccgat tgccctgcgt
     1861 tggtatgggc taatgtattt agccggcttt gcctttgcct actggctggg aatgagacgg
     1921 gcgaaaaatt ccaatggagt ttggacaacc gagcaagtgg atagcttagt ctatacttgc
     1981 ttctggggcg tagtattagg tgggcgtatc ggcgatgtgt tcttttatag cttcgaccgt
     2041 ttcttacaag atccgctcta cctctttcgc atttgggaag gcggaatgtc ctttcacggc
     2101 ggtttaatcg gcgtcattgt agcaatgatt tgggtttcct accgccaaaa acgcaatttc
     2161 tggcagacag cagattttat cgcaccgcta gtaccgttcg ggctaggtat ggggcgtatc
     2221 ggcaatttca ttaacgatga actttggggg cgtgtaaccg atgtgccttg ggcggtgtta
     2281 tttccaagcg gtggctatct tccccgccac ccgtcacagc tttatgaagc ctttttagag
     2341 ggtgtggtgt tactctttat cctcaactgg tttattcaaa agcctcgccc ggcaggtgcg
     2401 gtttcagggt tatttttaat cggctacggc acattccgct ttattgtgga atacttccga
     2461 gaaatcgacc ataacgtcaa taccaccgca gatttaatca ctcgtgggca actactctct
     2521 ttaccgatga ttatcggcgg catactgatt atggcgtggg cgtataaagt aaacaaagtg
     2581 aaataaacgt aggggcaagt caatatttgc cctcattaca aaaattgcaa aaaatttgca
     2641 gaaaacgacc gcttgtgttt gaaggatata ctatgagtaa aaaatacaaa gtcatcggtt
     2701 tcgacttaga cggaacgcta atcaacaccc tccccgatct cactcttgtc gccaattcaa
     2761 tgtttctaga agccggtttg ccaacggtta cacaagaaaa agtgctgact tggatcggta
     2821 aaggggcaga tattttcttc caaaatgcgg ttgctcacac cggcagaatt ttccaagccg
     2881 atgagttggt gaaaatgcga gtgagctttg ataaatacta cgccacctat gtgtgcgagg
     2941 aaagcaccct tttcccaaat gtgaaagaaa ccttagaaat cctcaaagca aacgactacc
     3001 cgctggtggt gattaccaac aaaccgacca aattagtcga gccggtgctt tccgctttcg
     3061 gcatttatca tttattcgat gaaacccttg gtgggcaatc gctccccaaa atcaaaccgc
     3121 accctgaccc gatgtttttt atctgcgaaa aattcggcat taccccatca gaattacttt
     3181 ttgtcggcga ctcagaaaat gacgtgattg ccgccaaagc cgccggttgt gatgtggttg
     3241 gactaactta cggctataac tacaatgtgc cgattgaaca atctaacccg acttttgtga
     3301 catctgaatt taaagatgtg ttgaaaattg tggggttata aagaaaacat ttgttttctt
     3361 ttgatgtttg ctttagactt tccgaaccta aagatttaaa ggagtaaagt atgccaacaa
     3421 tcgcattacg tcaggtcggt ggctcggttg caatggtgat tccgcctgct tttttagagg
     3481 cactcaagct ctccgtcggt agcaaagtgg atttgtcttt aaacgacaac caactcgtga
     3541 ttaatcctgc ccgcccaaaa gtgaaataca gcttagagga attattaaga caatccgatt
     3601 ttgaggcagc aaggaatgaa gaaagccaat cgtggttatc cgagccggca ataggaaaag
     3661 aaattttatg aaacgtggcg atatttactg gctcgatctt gatccaacct taggacacga
     3721 acaatcaggc ttccgccccg ttgtaattgt ttccgcaact gcatttaatc tagccagtaa
     3781 actgcctgtg gttgtgccga ttactaacgg tggagctttt gctgaaagac tgggctttgc
     3841 cgtttcatta aaagatgccg gcttaaaaac aacagggatt attcgatgcg atcaacctcg
     3901 cacagtcgat attgcacaac gccatggaaa atttatcgaa gccttacccg aaagcctact
     3961 tgatgaaatt atggcaaggg tttcagtgat ttttgagtaa gatacaagcg gtctgatttg
     4021 ctctattttt tgcaaaatca tcaataaatc tgaccgcttg tacttttatc ccttcccaat
     4081 aaccgttaag ataacctata tttttcaaat caaacgagaa gataaaatga gcaaacctat
     4141 tgtatttagt ggcgtgcagc cctcaggtga attgactatc ggtaactact taggggcgtt
     4201 acgcaactgg gtgaaaatgc aagatgatta tgactgtgta tattgcattg tcgatcaaca
     4261 cgccattacc gttcgtcaag atccggagtc gctacgcaaa tcaactttag atgtgttagc
     4321 cctgtattta gcttgtggca ttgatccaaa caaaagcact atttttattc aatcccacgt
     4381 gccggaacac gcccaattag cttgggtgct gaactgttac acctatttcg gtgaaatggg
     4441 gcgtatgacg caatttaaag ataaatcggc acgctatgaa gagaatgtca atgtgggctt
     4501 atttacttat ccggtattaa tggcggcaga tattctgctt taccaagcca accaagtgcc
     4561 ggtaggcgat gaccaaaaac aacatttgga aatcacccgt gacattgcca accgtttcaa
     4621 tgccttatac ggcaaaaaag attccgaagg caacctgatc gagcctgttt ttgcagtgcc
     4681 ggaagtcttt attgcgaaat ccggggcaag agtgatgtcg ctacttgagc cgaccaaaaa
     4741 aatgtcaaaa tcggacgata atcgtaacaa tgtgattgga ctattggaag atccgaaagc
     4801 ggtagcgaag aaaatcaaac gggcaatgac cgatggcgat gaaccgccag tagtgcgtta
     4861 tgacgtacag aataaagccg gtgtctcgaa tttattggat attctctccg ccattaccgg
     4921 caaaaccatt gccgaattag aaaccgaatt tgaaggcaaa atgtatggtc atttgaaaac
     4981 agaagtagca gataaggttt ccgaactatt aaccaatctg caagaacgtt atcaccacta
     5041 ccgccaagat gaagcattat tggaaaaaat ctaccgtgaa ggggcagaaa aagctcgagc
     5101 tcaagccaaa gcaacgttag ataaggtata tgaattagtc ggttttgtgc gttactaaca
     5161 cttaatgaaa aatgctcttg aggattcaag agcatttttg caaaaaagaa ccgattgtca
     5221 attatttacg ggcgttataa atcttcattg cttccggcac aaggcgggct aagttttctt
     5281 gacggtcagc atttgatggg tgagttgaga aaatggacaa gcctgaatcg ccattggctt
     5341 tactcatttt tacccaaaca ttcggcgctg ctgatgggtt gtagcctgat tctgccatta
     5401 aaattaaccc aacttcgtcc gcctcagtct cttggctgcg agagaatggc ttggtggcaa
     5461 tcaaatccac gccagtgctg agtaaacctt cggtgcttac gcctgttgag gcagtcacag
     5521 ccacatctgc aatagcacct acaatactgg taatagcgct aaccgtacgg tctgatttac
     5581 cgtgttcttt cagtgcgtga gccatctcgt gtcccattac cactgcaatt tcatcgttcg
     5641 ataaattaag tctttccact aaccccgtgt aaaacgccat tttaccgccc ggcatcgccc
     5701 acgcattgag ttcatccgat ttcaatacgg taatttccca atgaaaaggt acaccagtct
     5761 ggttggctct ttccgcataa ggcttcattt tatgaaaaac agtgtgaatc cgcttagcgg
     5821 tggcagacga attgtcaatc gcattttgct gacgagcttg ggttttcact tgagcataac
     5881 tttgagccgc ttcttggtta atgctttctg ttgtggcaca agcggtaatc actgttgcta
     5941 aaaaaactga ataacttaat tttttgatga atttcatttt atatcccaca aaaaatgcca
     6001 aacaaatttg cttggcattc aaaaataaat aattatttct tagcacgctc gaatgattct
     6061 aagatttctt ggcgagcttc gtttacatcg ccccaacctt ctactttcac ccatttgccc
     6121 gcttctaatg ctttatagct ttcgaagaag tgctggattt gtgcttttaa taacgccggt
     6181 aagtcaccta catctttgat gtggtcatac tctttactta atttggtgtg tggaacagca
     6241 accacttttg catcaccgcc ggcttcatcg gtcattttta acacaccgac aggacggcaa
     6301 cgaatcaccg aacccggttg taacggatat ggtgttggca ctaatacatc aaccgggtca
     6361 ccgtctgatg ataaggtgtt attcacataa ccatagtttg ccggatagaa cattgcggtt
     6421 gccataaagc gatccacaaa taatgccccg gtttctttgt ccacttcata tttgattgga
     6481 tcagagtttg ccggaatttc aattacaaca taaatatcgt ccggtaattc tttaccggca
     6541 ggaactaatt ctaagcccat tttggtttcc ttctataata ggtgaaataa acggcgtgat
     6601 tatagcctat tttcagaaaa agataaaagc ccgtttctct tttcattttc cgccaacaaa
     6661 tccggctaat tttttgctaa attgcttaca aaccgcacac tcaaccattt ttcgataaaa
     6721 aaaacttgcc aaagttttaa aaatccgtaa gatatgcact cgttacgcaa gattatgggt
     6781 cattagctca gccggtagag cagcggactt ttaatccgtt ggtcgaaggt tcgaatcctt
     6841 catgacccac cattaatctt caaaacacct ctaagggtca ttagctcagt cggtagagca
     6901 gcggactttt aatccgttgg tcgaaggttc gaatccttca tgacccacca ttaatttcaa
     6961 aactcctctg agggtcatta gctcagtcgg tagagcagcg gacttttaat ccgttggtcg
     7021 aaggttcgaa tccttcatga cccaccattt actcaaatcc catttgcaaa tttttctgaa
     7081 aaaacaaccg ctttatagta gaattcagtc aatttttatc acttttattt tattcatcaa
     7141 tgctaaaaaa aatccttatt gcattaggct tagccggcgt tattgcttgc ggtggactgt
     7201 tttacggcta ccaaaaactc acctctttag ccgaacaccc gatcacggtt cagccgaatc
     7261 aacttttcgt attggaaaaa ggcgtttcca gccaaaaatt agcggctctg ttagaagagc
     7321 aaggcattgt cacccacgat gacgccgatc ttatccctta tttaatgcga ttataccctg
     7381 agttaagcaa attcaaggct ggtgcttatt cactcgcagg cttaacgaca gtaaaagatt
     7441 tgctcgctca tttaagctcc ggcaaagaag tgcaacttaa cgtgcaattt attgagggca
     7501 aaacttttaa aatttggcgg gaacaactag cgaaagccga ttatttacag cacacgctac
     7561 aaggcaaatc ggaaaaagaa atcgctgact tactcggcat tccacacgaa aaaatcgaag
     7621 gttggatagc cccggatact tacaactaca cgcccaattc caccgattta gccttgctta
     7681 aacgggctta tcaaaagcag aaaacggcgt tggaacaggc ttggaaaaat agagcggaaa
     7741 atctaccgct tgcaacacca tacgaaatgc tgattcttgc ctcgattgtg gaaaaagaga
     7801 ccggcattgc gaccgaacgc ccacaagtgg catcggtctt tattaaccgt ttacgcaaca
     7861 atatgcgact gcaaaccgat ccaaccgtca tttacggaat gggcgatcgt tataacggca
     7921 atatccgccg caaagattta gaagaggcaa cgccatacaa tacttaccag attgacggct
     7981 taccgccaac gccgattgcc atgccgagtg aagcttcgat taaagcggtt gccaacccgg
     8041 ataatacacc ctacctctat tttgtggcag acggcacggg cggtcataaa ttcagcaaaa
     8101 cactgaatga gcataataaa gcggtggcag aatggattca aattgaacgc aaacgcaaaa
     8161 atgaacaaaa atagtgagaa caatatgcgt ggaaaattta ttgtaattga aggcttagag
     8221 ggcgcaggca aaacgactgc tcaccaagtg attctcaaag agttggaaca agccggcatt
     8281 aatgacgtag taatgacccg agaacccggc ggcacccccc ttgccgaaaa gttacgccat
     8341 ttaattaaac acgaaaccga agaagccatt aacgataagg ctgagctgct aatgctctac
     8401 gctgctcgtg ttcaattagt cgaaaatgtg atcaaacctg cccttgccga aggcaaatgg
     8461 gttctgggcg accgccacga tatgtcttcc caagcctatc aaggcggtgg acgtaagttg
     8521 gatcgtcact tactcgaaac cttaaaagaa tcggtactcg gcacattcga gccagattta
     8581 acgatttatc tcgatctcga tccggtgatt ggtttagaaa gagccagagg cagaggcgaa
     8641 ttagaccgca tcgaacagca aagccttgac ttcttctacc gcacacgaga gcgttattta
     8701 gaattaaccc aacacaacga taaagccgtg atcatcaatg ccgaacaacc catcgaacag
     8761 gtgactgcgg atattcaaca agtaattaga aactggctga aataatgtac ccttggctcg
     8821 aaagcactta tcaacaactt accgattcat ttttacaggg tcgaggtcat cacgccttac
     8881 tgttcaaaac agatgttggt cttggtacag acaccttaat ctacaaggtg gctgaatggt
     8941 tgctttgcag ccaaaaactc cgccatcaac cttgtcttga atgcaaaagt tgcctgctta
     9001 tgcaaagcaa caaccacccc gatttccaca ttcttgagcc gattgaagac aaagatattg
     9061 gtatcgacca agtgcgtgag ttgatttata aactccagaa tttcgcccaa caagggggaa
     9121 atacggtcgt ttatattcga ggagttgatc gtttaaccga ggcaagcagt aatgccctgc
     9181 tcaaaacctt ggaagagcca cgtgaaaacg tctatttctt gctcgaagca ccgcttcaag
     9241 caacagtttt agctaccatt caaagccgca gccaaacttg ggtactttac gccccacaag
     9301 cctcagaagt aatggcttgg ctacaaacac aatttccggc aatctcaacc gacgacttag
     9361 agattgcact ccgcctttgc cactcacgcc cactaatttg caaaaatttt atcgaaaatg
     9421 accgcttgca acagcgaaaa acctttttgc aaaccttctg gcgtttctac aaaagccacg
     9481 atgtcttctt gttattccaa gccttcgccc aagaaaaaga gctgatttta cagcaactcg
     9541 aatggctgga aagtttcttt agcgatgccc taaaagctaa aatggcaatc agccacggtt
     9601 ggctaaaccc ggatctccaa aacggcattc tcccttttag ccaacaacta acgacccacg
     9661 gcttacttaa agggcatcaa attctccaac aaacccagcg ggatttaacc gaaattaacg
     9721 ccgtcaatcc ggaattgatg ttgctagatt gcctgacgaa gttggttttg gtgtttgagt
     9781 aaaagccctc tctcatagaa ggtagctttc aattagccgt aaaaagaggc ttctagccct
     9841 agaatatagt ttataacttg caaaattatc tatctttgtt attgtatgaa taattcacac
     9901 aaggagaata ttatggcaag aacaaccagc gtgactttag gcgaaccttt aaatgatttt
     9961 gtaaatgaaa tggtggcaag tgggcgttat ggctctacca gtgaagtagt gagaaccgct
    10021 ttacgaatgc ttgaggaaaa agagcaatac ttagctcagt tgcgacaagc cattgatgat
    10081 ggtgatgcta gtggttatgc aaccgaaacg attgatgaaa tgatgttgag agtaaaggcg
    10141 gaacttaatg tataaacttt ctgctcaagc actccgagat caagagaaac tttttcgtta
    10201 cagtatctct caatttggtg ttcaacaaac agaatcttat tttgcagatc taaagcatat
    10261 tttgaattta ttagctgatt ttccaacaat gggaacttct gcggaatata ttcgtggtgg
    10321 cttgcgaatg cacacacatc aatctcatac aatttattat cgaataaaag cagatcatat
    10381 atttattgtt cgcatcttac ataaccaaat gaatcataaa cgctatattt agaaaaatga
    10441 aagacttatt tatcatagac tcacattgtc atctagattc tctagactac gaaactcgtc
    10501 acaaaaacgt ggacgaagta attgaaaatg ccaaagcccg aggggtgcat cattttatct
    10561 cggtttgcac tacagttggg cgttttgaag caatgaaaaa cttaaccgcc catcgtaatg
    10621 acgtttcact ttcttgtggc gttcacccgt tgaatgtgga agatgaacct tttgattacg
    10681 ataaattatt tcagttcgct caagatccga aagtggttgc cattggggaa actggtttgg
    10741 attatcacta cacaccggaa accaaagcgt tgcaacaatc gcttttcgtt caacaaattg
    10801 aaattgcaaa caaactcaat aagccactca ttatccattc tcgttcggca cgccaagata
    10861 cgatggatat gttggaacaa aataatgccg aaaaatgcgg tggggtgttg cattgcttta
    10921 ccgaagattg ggcaatggca aaacgggctt tagacatcgg cttttatatc tcaatttcgg
    10981 gcattattac tttccgcaat gcagaagaac tccgagaagt cgtgcgaaaa gtgccgttag
    11041 atcgcttatt ggttgaaaca gattcccctt atcttgcccc gattccttat cgtggtaagc
    11101 cgaaccagcc ggcgtatgtg cgggaaactt gcgaatatgt ggcgacatta aaaggcgttt
    11161 caactgaaga attagctcgc attacaaccg aaaatgtgca acacttattc aacattaaat
    11221 tataaggaac taatatgaga gcaatactta agtattttct gattcttgtc tcactatcat
    11281 tttttattgt attaggcggt gcagttaatt atgcaatgcc aagttatgaa gataccgtag
    11341 taacaggtat ggaagtacgc cgaatggata aagatggtat tatcagcaaa gcgaatcctg
    11401 ctgatggcga ggtgcgcgat gtttatttct tattcacgga agatcctgaa accaaaaagg
    11461 taatggttta tcgtaatgaa gataccggct gggggttgcc tccttatttc aaattcggat
    11521 ctgccgacat tcaagccaaa gctcaagcct acgctaacga aaaacaacgt gtgcaaatta
    11581 aatattacgg ctggcgaatc aattggctaa atgaatttag aaatattgtt tcaatcaagc
    11641 cattagaaga agcagaaaca gtcagcaaac caattatgag ctatgtactt tatgccgtat
    11701 tggcgttctt gttcttcctc tcagtacaat ttattcgtgg catttttaaa gattaatgcc
    11761 gattaacctt tgacaaacgg ttcgattttg taaaaaattg aaccgttttc ttttttatca
    11821 acaatatcaa atgacgtact attttattgc cgatctccac ttaaatgaaa atcagcctga
    11881 aattacccac ctctttttgc aatttatgca agaaaaagca ctacttgcag aagcggttta
    11941 tatcttaggt gatctgtttg atttctggat tggcgatgat gagcagtctg aactcatttc
    12001 aaccgttaaa caggcaatca aaaccctcac tacaagcggt acaaaatgct attttatttg
    12061 cggaaatcgt gattttttaa ttggcaaacg attcagcgaa caaacgggta tggttatttt
    12121 gcctgattat cacgttatag atttattcgg cacatctacc ttgctttgcc acggcgatac
    12181 gctttgtact gatgatgtga aatatcaaca atttcgtaaa aaagtccacc aaaaatggcg
    12241 acaagctatc tttttgatgt ttccgctttc ttggcgaatt cgtattgcgg agaaaattcg
    12301 atccaaaagc aaacaagaaa agcgtgataa atccgctgaa attatggacg taaacgctga
    12361 agttaccgct caaattgtcg caaaatacaa tacaactcaa cttatacacg ggcatacgca
    12421 tcgacaagca atccatcagc ataatggatt tacccgcatt gtattaggcg actggaaaga
    12481 aaattacgcc tctattttaa gcgtaaccga gcaaggagct gaatttatat gattaatgtg
    12541 attattcctt gctataacgc cgaaaacacc cttgttcgtg cagtgcaaag tgtattaaat
    12601 cagtctggat taaatctatt gtggcttatt gatgatgctt caacggataa tacgcttgcc
    12661 ttagctaaac atttacaagc ccaagtgcct gataaaatta gagtggaaca aatgcctcaa
    12721 aatggcggtg ttgccaaagc acgcaactgg ggagcattac aaagtgaaac agatttaatt
    12781 gcctttttag atgctgatga tgcttatgaa aacggggcat tgcaagttgc tgaaaagatt
    12841 tttgagttta aaccggaaac cggcttggtt cggttagcat taaaaccggt aacttagatc
    12901 aacgttacag ctctcaccct aattttgagt tagcttggca atatatgcaa atgactggtg
    12961 caggcaacac tgtatttcgg cgtagtttct tcctcgcttg tggcggcttt ccacaacatc
    13021 agctcttcca agaattaggt ggagaagatg gtgctttagg catagccact acacaaatta
    13081 gctctgtagc tactgcattt aatgatgtcg gtgttttaca ttactgccga gagggtatgc
    13141 acgcagaaag attattaaat gcgattttat tcaacgaaaa agatcccaat gttactgaag
    13201 aaaaagttaa acaagccaac ctaattaccg aaaatattgt taataatatt agaaatctac
    13261 aagactgtct taatagtaaa gggattggta ttaagcctta tacgcttgaa tggagctaaa
    13321 aaagcatttg cagattttcc ctaaaaaaa
//
LOCUS       DANLYI010000049        13349 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.049, whole genome shotgun sequence.
ACCESSION   DANLYI010000049 DANLYI010000000
VERSION     DANLYI010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 13349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13349
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.049"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            259..334
                     /locus_tag="PXD81_001045"
     tRNA            259..334
                     /locus_tag="PXD81_001045"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:292..294,aa:Trp,seq:cca)
     gene            351..536
                     /locus_tag="PXD81_001046"
     CDS             351..536
                     /locus_tag="PXD81_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5537082.1"
                     /translation="MYNLDDAIQECREKFGEDKPYFSEAGTLTFAGCKALSRLRDEKL
                     GIKAPPNFPFPRVEDLS"
     gene            562..732
                     /locus_tag="PXD81_001047"
     CDS             562..732
                     /locus_tag="PXD81_001047"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5537083.1"
                     /translation="MTEQLNTQSKSGEEDQEKSQDRYAVGWPSRRISDCVQHFRSRKI
                     YPFASNNDVKQG"
     gene            875..1120
                     /locus_tag="PXD81_001048"
     CDS             875..1120
                     /locus_tag="PXD81_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5537084.1"
                     /translation="MKLMKDMGTQSYIWSGFSTWLAWLGEQQNLMLLSLAIGIVTAIA
                     GLIQRFDEWRRRKREDERAEELHQAKMARLRKGLSDE"
     gene            1113..1682
                     /locus_tag="PXD81_001049"
     CDS             1113..1682
                     /locus_tag="PXD81_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HDL5537085.1"
                     /translation="MSKSLKYGSGIVCGIAAIITLVQYQHPEIRTNQAGLEIIGNAEG
                     CRRDPYKCPADVITVGIGSTEFGGEKIDPNRIYSDKEIAERWAKDLKIAESCVNRHFN
                     GKDMNDNQFSGMTSAVFNMGCYNMRFYRNKQGQYVQTTIHKLAVNKQFEEMCHRLPDF
                     IRASGKVLNGLVIRREKEKALCLTGLVAR"
     gene            1655..2005
                     /locus_tag="PXD81_001050"
     CDS             1655..2005
                     /locus_tag="PXD81_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2570 domain-containing protein"
                     /protein_id="HDL5537086.1"
                     /translation="MLNWISGTIAIVILGLCVWLWGQSQMIDSLRAENRAQAQTIEQQ
                     QKANQRLTDSLEQERQAVEKIQKIANELRNKVEMAKNEITSILAQDSCAKADLPNGVA
                     DSIKRLHQQNNHKH"
     gene            2010..2183
                     /locus_tag="PXD81_001051"
     CDS             2010..2183
                     /locus_tag="PXD81_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5537087.1"
                     /translation="MYPPAAYLVPCGRTEFSGRTYGDTVEYLVKVMGERDLCANQIDR
                     IREWQAQTKQGFK"
     gene            2575..3099
                     /locus_tag="PXD81_001052"
     CDS             2575..3099
                     /locus_tag="PXD81_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HDL5537088.1"
                     /translation="MTKKGEVKATSKGVGKLTDKQKRFVEEYLIDLNATQAAIRAGYS
                     EKTAYSIGEENLRKPEIRSAIQEAQNKRSERTQITQDDVLNGLLEVIAMSTGKKIVTE
                     TDVAKNDNGELVGFDIAKTKFEPAAANKALELLGKHLGMFKDKVDLINSDGSLNRPMV
                     IELIAPSLDDESTD"
     gene            3083..4303
                     /locus_tag="PXD81_001053"
     CDS             3083..4303
                     /locus_tag="PXD81_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HDL5537089.1"
                     /translation="MKAQIELPPKLLPVFAKDYRYKGAYGGRGSGKTRAFAKMSAVSA
                     YKRAMRGESGVILCGREFMNSLEESSLEEVKQAIKSEPFLANFFDVGEKYVRTKDGRI
                     SYVFSGLRHNLDSIKSKARILIAWIDEAETVSETAWRKLIPTVRENNSEIWVTWNPEN
                     RGSATDTRFRQNPPENSCIIEMSYQDNPWFPDVLEQERLNDKQRLDDATYRWIWEGAY
                     LEASEAQIFRGKYQEMEFVPNPDFDGPYYGLDFGFAQDPTAAVKCWVFNGDLYIEFEA
                     GKVGLELDDTADFIAKGVPQIADHVVRADSARPESISYLKRHGLPRIIGVEKWKGSVE
                     DGIEHIKSYGKVFIHPRCQQTLNEFRLYSYKTDRLSGDVLPVVIDAHNHFIDALRYAL
                     TPLMQVKSAKGVLL"
     gene            4300..5676
                     /locus_tag="PXD81_001054"
     CDS             4300..5676
                     /locus_tag="PXD81_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1073 domain-containing protein"
                     /protein_id="HDL5537090.1"
                     /translation="MSLEQDRLAFLAEALGLGNIKRRTLWKEFGYPNTLTFNHFLKAY
                     KRNSIAFAAINRLLDGCWVDCPVIVEGEQKNESKQTTEWESKVERFMKRYWSAIKEAD
                     RRNLVGNYSALLLQVRDGQQWDQPILQGALSSIGEFGLVKLIPVWQSQLSVTEFQEDV
                     TADNYGEPLMYQFSESAFGKKSVSRNIKVHASRVILLNEGGDFNSPHSGVPLLEAGYN
                     KLVDLEKTSGGSAEGFLKNASRQLGIKLTKDVDLRQLEDSAKALGFSSFSDALNDKIK
                     KINSGTDSALITHEGDASVLSVAPADPKPTWEISANEFAASVQIPFTILFGQQTGRLA
                     SDEDKTDWANRCNGRRNGFLTDVITQLLNRLWFIGVLPMPKNSDVTVSWSDLLAPSEK
                     EKIANAQALASVATTSQSAFGFAAVTANEIRESLGFEPLPDNLIPPTIDDENEDQDEN
                     QATADSDK"
     gene            5630..6568
                     /locus_tag="PXD81_001055"
     CDS             5630..6568
                     /locus_tag="PXD81_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017357793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head morphogenesis protein"
                     /protein_id="HDL5537091.1"
                     /translation="MKIKTKIKPLRIPTNKADPLNMGVSVAKLFRHIDVIYSQIKRSI
                     KQQILQKMSLRLQANNFEYFPSYLTATELADLLETLQKIIDKEILSNGSQGENLWFDS
                     YIDEAALKGTQSAVTDLGLQSEAYRSQRNLSSIVFSPAYFNRLAIAHMASYSEWQGLS
                     DELRKSLAGVITEAVLNGKNVKETAREIRQKLDVSSKRAKRIAQSSQLAAYRRAEWQE
                     AEEAKEELGLNTKLLHFSALKATTRLAHAQRHGKYFDVAEVREWYSRDGNQFNCYCKQ
                     SVVVVNADGKTDIEPLLVGLAKERKQWVGALKNVKK"
     gene            6555..7925
                     /locus_tag="PXD81_001056"
     CDS             6555..7925
                     /locus_tag="PXD81_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016533290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2213 domain-containing protein"
                     /protein_id="HDL5537092.1"
                     /translation="MSKNNVNIVTVINSKNISTESIDGDQHLIIRGVVPIVDDVVMND
                     GLYPADEINKSYNSLEGNFMPLGHPKIEGKYVSAQDVRAVNKHHVGAWAKNVRKESGK
                     VLVDMYINRRFAEGSEQGKTLLERLDGMVTNTNAEPIHVSTGLILNKVKQSGKSKGKK
                     YSWIATNMSFDHIAILLDVPGAATPDDGVGIFVNQDGSEMGVENVNLTQAADYRTEKL
                     FDKVRFYLMANSDLSFEEIHRALREAIRPEGKDKWSFWVEAVYPKYFIYSDDNSGKKY
                     KRSYFIDENDNVQLTSEPVEVVKKIGYEEITTNEEHNPMKTKILAVLNAANVKTEGLN
                     DDQLLEAYNKLQADKGKEPEKPKNEDDSEIDEKIKKAVNAAIEPLQQALQANTDKEQA
                     AMRAAVKAKFNMSDAAVNALQGEVLTELYAQTQQSVGLNNNSANGNGDNQWDGYSLNQ
                     EEKGNE"
     gene            7918..8352
                     /locus_tag="PXD81_001057"
     CDS             7918..8352
                     /locus_tag="PXD81_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5537093.1"
                     /translation="MSNVIYRGSVKREPQTVNIVINDTSAPGAVVKLHNGKLEAAADL
                     KGRRFLLGNNRFKGQTIEQAYAKGDTATAFRLEPEQEYYAQIADGTYNFGDELTVKQA
                     GGKLTKAETGDVVLFFFDEESQRVISGGKGYADVVVANAYTK"
     gene            8367..9356
                     /locus_tag="PXD81_001058"
     CDS             8367..9356
                     /locus_tag="PXD81_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="encapsulin"
                     /protein_id="HDL5537094.1"
                     /translation="MLKFTKEQERFVINERINYDKKHALMAANSAGLLGNAMTLPKDV
                     WAEWDRDAVQIQREELVVFNDLAGISKSMPIGKLIHHFQTVSDSGSVNISLDGRSSAK
                     TDNVVLNYHGTPLPIIDSTFSFGWRDMSAAQSEGYQIDGAARANSLRKVAEKMEDLAL
                     NGDSSIVVGNAKLYGLRTAPNRMTNTHNLDLATATPKEIYDVFRDLISKFHAKNYYSP
                     VTLYVNYGDYFAMSTRDYSEQKSEGSILNKVMTIPQIAKIVPASRVPQNEILGLCKRS
                     DVYQVLNGMPLVTRPIARHNETDDYSFQIMAAVAVEFKFDDKGNAGYIQYTKS"
     gene            9368..9592
                     /locus_tag="PXD81_001059"
     CDS             9368..9592
                     /locus_tag="PXD81_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5537095.1"
                     /translation="MKWLLTHDSHELKKGDVYEGETLPLWLVGKAIEIEEKSFEVATP
                     SDHADGETGETGETGETGETGQKGKKDKGK"
     gene            9595..9972
                     /locus_tag="PXD81_001060"
     CDS             9595..9972
                     /locus_tag="PXD81_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5537096.1"
                     /translation="MAAEIELSEARTTLDELGFTSPNSLLELYVQQTNTLDQALNKSG
                     YSEETQKLIKLYLVAILAISSGARRIKSEGAPSGASRTFEYDPNALDNLKSAIRSIDP
                     KGCAESLLPKDKVVGFFDVVGGC"
     gene            9972..10316
                     /locus_tag="PXD81_001061"
     CDS             9972..10316
                     /locus_tag="PXD81_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5537097.1"
                     /translation="MSNLSNWSYTAKATLWQAKGKNDDGVLVFSTPQIISCDYGADRK
                     RARFEIGREQAVKNVIWTEFAHAKLGDYVLIGESEQADPILAGAEEIIHIQRFADTFD
                     RNRDDFALITGG"
     gene            10318..10686
                     /locus_tag="PXD81_001062"
     CDS             10318..10686
                     /locus_tag="PXD81_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HDL5537098.1"
                     /translation="MGIKVKGIRQVKQRLNQVVGEVQSQKATRAMFRILNAVAPLAAH
                     YTPVDTSTLINSQFTEMEVNGTRLIGRVGYSANYAAYVHDPNIKQNFRKPSAKKEFLT
                     SALNESKATIKSIVKEELSL"
     gene            10683..11063
                     /locus_tag="PXD81_001063"
     CDS             10683..11063
                     /locus_tag="PXD81_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5537099.1"
                     /translation="MISYVKAFKQWLEAHSLADGYVVQLYQWEDTQKAKPVIVIQPNS
                     GSPQVSDLSSEHYLLISLVAGKNSGYTIEERARAIMSKILVEPFASFGYIESMGGLPA
                     PIFTEDNRMIFRLPLRIISTNQEE"
     gene            11067..11549
                     /locus_tag="PXD81_001064"
     CDS             11067..11549
                     /locus_tag="PXD81_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL5537100.1"
                     /translation="MGSPSPQNNAENLVVGRAVVLEYGTTDAKPQESEWKVAGAMTTK
                     SWDFSPNSVTSEADDAGGFPETLITNSDFSISGDGEWRKRPKSKELGIKDLVVLYANA
                     VKNRTQPYIWVRLKYAGLTILGKMVITALSSEAPTNDLVKFSIEFKVGDSSTIEIAAA
                     "
     gene            complement(11589..12005)
                     /locus_tag="PXD81_001065"
     CDS             complement(11589..12005)
                     /locus_tag="PXD81_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HDL5537101.1"
                     /translation="MFYPALFTPAEEGGFVVTFPDLPEAITQGDTFEEAMEMAEDVLL
                     SCVEIYFDEDRRFPLTRPVNTDEVPVFMPESIYAKVLLHNTMQEQAVSKAEIARLTNI
                     RPPEVQRILAPRHITKIDTIGRILASLGKPLQLSLG"
     gene            complement(12061..12237)
                     /locus_tag="PXD81_001066"
     CDS             complement(12061..12237)
                     /locus_tag="PXD81_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010848235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HDL5537102.1"
                     /translation="MKQSEFLRWLKANGVEVENGSKHLKLYYKGKRSHLPKHPSQELK
                     TGLVEGVKKQLGLK"
     gene            12333..13004
                     /locus_tag="PXD81_001067"
     CDS             12333..13004
                     /locus_tag="PXD81_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5537103.1"
                     /translation="MKMVSYPITEIGEMLIATDGRDFLFRPSFKNIYKIGSPQEIVKI
                     YALLHGCEIREILPHILTKSESHQSYFWSVLNSPVFGRKILQNAMIVMSCCCDDDISL
                     LIGEWLPSKSGVRYKLGKMPPETIINFAKNLMGHGVIGCCELPKKEQSAKNEFSESFD
                     AVEYISLARTHFNMSREDAENLTMTELQQLLKSQMPAENNSYGQFTEEEYDRIMAEYE
                     KRRAA"
     gene            complement(13019..13252)
                     /locus_tag="PXD81_001068"
     CDS             complement(13019..13252)
                     /locus_tag="PXD81_001068"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5537104.1"
                     /translation="MKKDCTKQDFANWLNENQEVCWIKEVSIERILSCVDDYVAICNA
                     RSKDEFELLNDIRELFYSAHYKDFTPVLSGRFF"
BASE COUNT         4025 a         2659 c         3200 g         3465 t
ORIGIN      
        1 taaactgaaa aaatatcaat tttccccttg attttagtgc gtaacaagtg tatattttgt
       61 gctaatggtg gacgtagtat aaatgatgtt taccaatgtt agttaaagac gcagcctgaa
      121 gtaatattca gttcggagga gaataactag aaacgacgtc gagtatagtt atttcagcgt
      181 ttgcgtggca gtatgccgac cgaattagtt taacagccct gagcagaaat gcttggggct
      241 tttttgttta cggattttaa gggtgtagct cagttggtag agcagcggtc tccaaaatcg
      301 ttggttgttg gtttgagtcc aaccaccctt tccaaatgcg agtaaatgtt atgtataacc
      361 ttgatgatgc aattcaagaa tgcagagaaa aatttggtga agataagcct tatttttctg
      421 aagctggaac acttactttt gctggttgca aagccctctc tcgattaaga gatgaaaagt
      481 taggaattaa ggctccgcca aatttccctt tcccaagggt tgaagatcta tcttagctac
      541 aaaaatcaat gagggattgc tatgacggaa caactaaata ctcagtcaaa aagcggtgaa
      601 gaagatcaag aaaaatctca agatcgctat gccgtgggct ggccatctcg acgtatatca
      661 gattgcgttc agcacttccg aagtaggaaa atttatccat ttgccagtaa taatgacgtg
      721 aagcaaggtt aaatgcttct ggtggtacag agtaaattca atcattttta ccctctgcta
      781 gtttatttgt tggggaacag tattctagca gaatttttaa ccaaagctca gtctcacgac
      841 tgggcttttt tattgcctcg aaacggggtg gagtatgaag ttaatgaaag atatgggaac
      901 gcagagctat atttggtcgg gatttagcac ttggctggcg tggctcggcg aacagcagaa
      961 tttaatgtta cttagtcttg ctatcggtat tgttactgcc attgcagggc ttattcagcg
     1021 atttgatgaa tggcgtaggc gtaagcgtga agatgagcga gcggaagagc ttcatcaagc
     1081 gaaaatggca agattgagaa aggggttaag tgatgagtaa aagccttaaa tatggcagtg
     1141 gtatcgtttg tggtatcgct gccattatta cattagtcca atatcaacat cccgaaatcc
     1201 gcactaacca agctggatta gagattatcg gtaatgctga gggttgcaga cgtgatccgt
     1261 ataaatgccc tgccgatgta atcacggttg gcattggctc aacggagttt ggcggtgaaa
     1321 agattgaccc aaatcgtatt tattctgaca aagaaattgc tgagcgttgg gcaaaagatt
     1381 tgaagattgc ggagagttgt gtcaatcgtc attttaatgg taaagacatg aacgataatc
     1441 aattttcggg aatgacgtcc gctgtattta atatgggttg ttataacatg agattttatc
     1501 ggaataaaca aggtcaatat gtgcagacta ccatccataa actcgcagtg aataaacaat
     1561 ttgaggagat gtgtcatcga ctacctgact ttattcgtgc cagtggtaag gtattgaacg
     1621 gattggttat ccgccgtgaa aaggagaaag cattatgctt aactggatta gtggcacgat
     1681 agccattgtg attttgggct tgtgtgtatg gttatggggt cagtcacaga tgatagatag
     1741 cttaagagct gagaaccgcg cacaagccca aaccattgag cagcagcaaa aagccaatca
     1801 acggctaacc gatagcttag agcaagagcg acaagcggtc gaaaaaatcc aaaaaattgc
     1861 aaatgagctg cggaataaag tggagatggc caagaatgaa attacatcaa tactggcaca
     1921 agacagttgt gctaaagctg atttgcctaa tggcgttgct gatagcatta agcggctgca
     1981 ccagcagaac aatcacaagc actgagtatt tgtatccgcc ggcagcttac cttgtgccgt
     2041 gtgggcgaac agaatttagc ggtagaacct atggcgatac ggttgagtat ctcgtcaaag
     2101 tgatgggaga gcgtgatttg tgtgctaatc aaattgaccg tatcagagaa tggcaagcac
     2161 aaaccaaaca agggtttaaa tagctaatta tctaattaac cgctattgaa aatgggagcg
     2221 taacagctcc ctttttgttt tgatggaaag taaatattac ataatttagc tatggtttcg
     2281 gggttggttg gataaaacac cgaggaaata cctaagtgat gtgatttata taaaacaaaa
     2341 ccccgaacat ttgcggtgtt cgaggttttt ttattaccca ttgaaacgga atgagtaaca
     2401 agttatggaa attattacat ttttaaccgt aaccatcaag gagattctta tggaatatgg
     2461 tttatggcaa ataagcctag cagtaacagt ccctattttg gcgttcgtgt cgccaaagct
     2521 gattaatgct attgctaatc tattaaatgc aatgaagtaa aggaatttaa cccaatgact
     2581 aaaaaaggcg aggttaaagc cacgtctaaa ggcgtgggta aattaaccga taaacagaaa
     2641 cgatttgtag aagagtatct cattgacttg aatgcgactc aagctgctat tagagctggg
     2701 tacagcgaaa aaacagcata ctcaatcgga gaagagaacc tgagaaaacc tgaaatcaga
     2761 agtgcaattc aggaggctca aaataaacgc tcggagagaa cccaaattac acaagatgat
     2821 gtgctaaatg ggttgcttga agtgattgca atgagtactg gtaaaaaaat tgtgacagaa
     2881 actgatgttg caaaaaatga caatggtgag ttggtcggtt ttgatattgc aaaaacaaaa
     2941 ttcgaacctg ccgccgcaaa taaagctctt gagttactcg gtaaacattt aggaatgttt
     3001 aaagacaagg ttgatttaat taattctgat ggttcactga atcgaccgat ggtgattgaa
     3061 ttaattgctc cgagtttaga cgatgaaagc acagattgaa ctaccaccta aactgttgcc
     3121 tgtttttgcc aaagattatc gttacaaagg tgcttatggt gggcgaggtt caggcaagac
     3181 tcgggcattt gccaaaatgt cggcagtgtc tgcttataaa agagcaatgc gaggggaaag
     3241 tggcgttatt ctttgcggtc gtgagtttat gaactcattg gaagaaagct ctcttgaaga
     3301 agttaaacag gcgatcaagt ctgagccttt tttagcaaat ttttttgatg tcggcgagaa
     3361 gtatgttcgc actaaagatg ggcggatttc ttatgtgttt tccggacttc ggcataactt
     3421 agatagcatc aaatctaaag cccgtatttt gattgcgtgg atagatgaag cagaaacagt
     3481 aagcgaaact gcttggcgga aattaattcc aaccgtgcgt gagaataatt ccgaaatatg
     3541 ggtaacttgg aacccggaaa atagagggag cgcaacagat actcgcttca ggcaaaatcc
     3601 acctgaaaac agctgcataa ttgaaatgag ttaccaagat aatccttggt ttcctgatgt
     3661 gctcgagcaa gagcggctaa atgacaaaca gcggcttgat gatgcgactt atcgctggat
     3721 ttgggaaggt gcttatttgg aagcgagcga agcgcaaatc tttcgcggta aatatcaaga
     3781 aatggaattt gtgccgaacc ctgattttga tggcccatat tacggcttag attttggttt
     3841 cgcgcaagat ccgacggcgg cggtcaaatg ctgggtattt aatggcgatc tgtacatcga
     3901 gtttgaagca ggcaaagtgg gcttagaact tgacgacacc gcagacttta ttgccaaagg
     3961 cgtgccacaa attgccgatc acgttgtgcg tgcagattcg gcgcgtcctg agtcaattag
     4021 ttacctcaaa cgtcacggct taccgcgcat tatcggtgtg gagaagtgga aaggctcggt
     4081 ggaagacggc attgagcata ttaagtccta cggcaaagtc tttattcacc cacgctgtca
     4141 gcaaacgctc aacgaatttc gcctttattc gtacaaaacc gaccgcttat cgggcgatgt
     4201 attgcctgtc gtgattgatg cccataacca ctttattgat gcgctacgct acgcattaac
     4261 gccgttgatg caagttaaaa gtgccaaagg agttttacta tgagcctaga gcaagacaga
     4321 ctggcttttt tagccgaagc cctagggctt ggcaatatca aacgaagaac gttatggaag
     4381 gagtttggct accctaatac cttaacattc aaccatttct taaaagccta taagcgcaat
     4441 agtatcgctt ttgcagcgat taaccgctta cttgacggtt gttgggttga ctgccccgta
     4501 atcgttgagg gtgagcaaaa gaacgaatca aagcagacaa ccgaatggga gtccaaagtt
     4561 gaacgtttta tgaaacgcta ctggtcggca atcaaagagg cagatagacg aaaccttgtc
     4621 ggtaactatt cggcgttatt acttcaagta agagatggtc aacaatggga tcagcctatt
     4681 ttgcaaggcg cgctatctag cattggagag ttcggactgg ttaaactgat ccccgtgtgg
     4741 cagtcacagc tttcagtgac agagtttcaa gaggatgtga ccgccgataa ttacggcgaa
     4801 ccgctgatgt atcaattcag cgaatctgcc tttggcaaaa aaagcgtgtc acgaaatatc
     4861 aaggttcacg caagtcgtgt aattttgctc aatgagggtg gcgactttaa ttcccctcat
     4921 tctggagttc ctctgcttga ggcgggttac aacaagctag ttgatctcga aaaaacatcg
     4981 ggcggcagcg ccgaaggttt tctgaaaaac gcaagccgac aattagggat caaattaaca
     5041 aaagatgttg atttgagaca gctcgaagac agcgcaaaag cccttgggtt ttcttcattt
     5101 tctgatgcgt tgaacgacaa gatcaagaaa atcaactcag gaacagactc agccttgatt
     5161 actcacgaag gtgatgcctc tgtgttgtca gtcgcaccag cagatccgaa gccaacgtgg
     5221 gaaatttctg caaatgagtt tgccgcctca gtacaaatcc ctttcacgat tttgttcgga
     5281 cagcaaacag ggcgattggc aagtgacgag gacaaaaccg actgggcaaa ccgttgcaac
     5341 ggtcgtcgaa atggttttct caccgatgtg ataacacaac tacttaatcg cttgtggttc
     5401 atcggtgtgc tacctatgcc aaaaaatagc gatgtcacag tgtcttggtc tgacttactt
     5461 gcaccaagcg aaaaagagaa aatcgcaaac gcacaggctt tggcttcagt tgcaactaca
     5521 tcgcagtctg cctttggttt tgccgcggta acagcgaacg aaatccgcga atctttaggg
     5581 tttgaacctc tgcctgataa tctgattccg ccaacaattg acgacgaaaa tgaagatcaa
     5641 gacgaaaatc aagccactgc ggattccgac aaataaagcc gatccgctca atatgggagt
     5701 gtcggttgcc aagctatttc gtcacattga tgtcatttac tcacaaatta agcgctccat
     5761 taagcaacaa atcttgcaaa agatgtcact gcgactgcaa gcaaataatt ttgagtattt
     5821 tccttcgtat cttactgcga ctgagttggc ggatttgctt gaaaccttgc aaaagattat
     5881 cgacaaagag attttgtcga atggttctca aggcgaaaat ctctggtttg actcctatat
     5941 cgacgaagca gcactaaaag gcactcagtc tgcggtcact gatttaggtc tgcaatcaga
     6001 ggcttatcgc agtcagcgaa acttatcatc aatcgttttc agtcctgcgt acttcaaccg
     6061 attggcgatt gcacacatgg caagttatag cgagtggcaa ggtttaagcg acgagttgcg
     6121 aaaatctttg gcgggggtga tcacggaggc ggttttaaac ggcaaaaacg tgaaagaaac
     6181 tgcccgtgag attcggcaaa aacttgatgt gtcatcaaaa cgggcgaagc gaatagcgca
     6241 atcaagccag ttggcggcat atcgtcgggc agaatggcaa gaggcggaag aggcaaaaga
     6301 agaacttggg ctaaacacca agctcttaca tttttcagcg ctcaaagcta ctacgcgatt
     6361 agcacacgcc caacgccacg gcaaatactt cgatgttgca gaggtgcgcg aatggtatag
     6421 ccgagacggc aaccaattca actgttactg caagcagtct gtggttgtgg tcaacgccga
     6481 cggtaaaaca gatattgagc ctctccttgt tgggttggct aaagaacgta aacaatgggt
     6541 aggagcatta aaaaatgtca aaaaataacg tgaacatcgt caccgtcatt aacagcaaaa
     6601 atatcagcac agaatctatt gacggagatc aacacctgat tatccgcggc gttgtgccga
     6661 ttgttgatga tgttgtgatg aacgacggac tttatcctgc ggatgaaatc aataaaagct
     6721 acaacagctt agagggcaac tttatgccgc ttgggcatcc aaaaatcgaa ggtaaatacg
     6781 tttcagccca agatgttcga gcggtgaaca aacatcacgt cggtgcgtgg gcgaagaacg
     6841 tacgcaagga aagtggcaaa gtgctggtcg atatgtatat caaccgccgc tttgcggaag
     6901 gttcagaaca gggcaaaacc ttgcttgaac gacttgacgg aatggtgaca aacaccaacg
     6961 ctgaacctat tcacgtttcc acagggctga ttctaaacaa agtcaagcaa tcgggtaaat
     7021 ccaaaggcaa gaaatatagc tggattgcga caaatatgtc gttcgatcat attgcaatat
     7081 tgcttgatgt gccgggcgcg gcgacacctg atgatggcgt ggggattttt gtaaaccaag
     7141 acggaagcga aatgggggtg gaaaatgtaa acctaacaca agcggcggat taccgcacag
     7201 aaaagctgtt tgacaaagta cgattttacc tgatggcgaa ctcagattta tcgtttgaag
     7261 aaattcatcg cgcgctgcgt gaagctattc gccccgaagg caaagataag tggtcatttt
     7321 gggtcgaagc tgtttacccg aaatacttca tctattcgga cgataacagc ggcaagaaat
     7381 acaagcggag ctattttatt gacgaaaatg acaatgtgca gctcactagc gaacctgtcg
     7441 aagttgtcaa gaagattggc tacgaagaaa ttacaaccaa tgaggaacat aatccaatga
     7501 aaaccaaaat cttagccgtg ttgaatgcgg caaacgtgaa aacggaaggc ttaaatgacg
     7561 atcagctctt ggaagcctac aacaagctgc aagcggataa gggtaaagag cctgaaaagc
     7621 ctaaaaatga agatgacagc gaaattgatg agaaaatcaa gaaagcagtc aatgccgcga
     7681 ttgagccact ccagcaagcc ttgcaagcta atactgacaa agagcaagca gcaatgcgtg
     7741 cggcggtgaa agccaagttc aatatgagcg atgccgcagt caatgctttg caaggcgagg
     7801 tattaactga gttgtatgcg caaacgcaac aatcggtcgg attaaacaat aattcggcaa
     7861 acggcaacgg cgacaatcaa tgggatggtt actcactcaa ccaagaggag aaaggcaatg
     7921 agtaatgtaa tttatcgcgg ttcggtcaag cgtgaacctc aaacggtgaa tatcgtgatc
     7981 aacgatactt cggcaccggg tgcagtagtt aaactccaca acggcaaatt agaggcggcg
     8041 gctgatttaa aaggtcgccg ttttttattg ggcaacaacc gctttaaggg gcagacaatc
     8101 gaacaagcgt atgcgaaggg cgatactgca acggcgtttc gtttagagcc tgaacaggag
     8161 tattacgcgc aaattgccga cggcacttat aactttggcg atgaactcac ggtaaaacaa
     8221 gcgggcggca aactgaccaa agcggagacg ggcgatgtag tgctattttt cttcgacgaa
     8281 gaatcgcagc gtgtaatcag tggcggcaaa ggctatgccg atgtcgtggt ggcaaacgcc
     8341 tacactaaat aagaggtaac aaagcaatgt tgaaattcac aaaagaacaa gaacgcttcg
     8401 tgattaacga gcggatcaat tacgacaaaa aacacgcgct aatggcagca aattcggcgg
     8461 ggttattggg caacgcgatg actttgccga aagatgtctg ggcggaatgg gatcgtgatg
     8521 cagtgcaaat tcagcgcgaa gaattggtgg tgtttaacga tttagcgggc atttcaaaat
     8581 caatgccgat tggcaaactt attcaccatt tccaaacggt atctgatagc ggttcggtta
     8641 atatctcatt agacggtcgt tcatcagcga aaaccgataa cgtggtgctt aactaccacg
     8701 gtacaccgtt gccgattatt gattccactt tctcttttgg ctggcgcgat atgtcggcag
     8761 ctcaaagcga agggtatcag atcgacggtg cagcacgcgc gaacagtctc cgcaaggtgg
     8821 ctgaaaaaat ggaagatctc gctttaaatg gtgattcctc tattgtggtt ggcaatgcca
     8881 agctttacgg cttgcgtact gcgccaaatc gtatgactaa cacacacaat ctggatttag
     8941 ccactgcgac accgaaagaa atttacgatg tgttccgtga cttgattagc aaattccacg
     9001 cgaagaacta ctactctcct gtaacgcttt atgtgaacta tggcgactat ttcgcaatga
     9061 gtacacgtga ttactccgag cagaaatccg aaggatctat cttgaacaaa gtgatgacga
     9121 ttccgcaaat tgcgaaaatc gtgccagcaa gccgtgtacc gcaaaatgaa attctcggct
     9181 tgtgcaagcg ttcggatgtg taccaagtgc ttaacggtat gccgttagtg actcgtccga
     9241 ttgcgcgcca taatgaaacc gatgattact cgttccaaat tatggcagcc gttgccgtgg
     9301 agttcaaatt cgacgataaa ggcaatgctg gctatatcca gtacactaaa tcctaaggcg
     9361 gtgcaaaatg aagtggctat taacacacga tagccacgag ttgaagaaag gcgatgttta
     9421 cgaaggcgaa acattaccgc tttggcttgt gggcaaagct atcgaaatcg aagaaaaatc
     9481 ctttgaagtt gcaacgcctt ctgatcacgc tgacggcgaa actggcgaaa ctggcgaaac
     9541 tggcgaaact ggcgaaactg gtcagaaagg caaaaaagat aaaggcaaat aactatggca
     9601 gcagaaatag agctatccga agcgcgaaca acgcttgacg aactgggttt cacttcgccg
     9661 aactctctgc ttgagcttta cgttcagcaa acgaacacct tagaccaggc gttgaacaaa
     9721 tcggggtaca gcgaagaaac acagaaactg atcaagctct atctggttgc tattcttgca
     9781 atttcatctg gggcgcgccg tataaagtca gaaggtgcgc ctagcggtgc aagccgaacg
     9841 tttgagtatg atccaaacgc gctagacaac cttaagtcgg cgattcgctc aattgatccg
     9901 aaaggttgcg ctgaaagttt gctacccaaa gataaagttg tcggcttttt cgatgtggtc
     9961 ggaggctgct aatgtcaaat ttatcgaatt ggtcctacac cgcaaaagcc acgctttggc
    10021 aggctaaagg aaagaacgat gacggcgttt tagttttttc tacgccacaa atcatttcct
    10081 gtgactacgg tgcagataga aagcgcgcac gttttgagat tgggcgtgaa caagcggtca
    10141 aaaatgtgat ctggacggag ttcgcccacg ccaaattagg cgattatgtt ctgattggcg
    10201 aaagcgagca agccgatccg attttggctg gcgcagagga aattattcat atacagcgct
    10261 ttgctgatac gttcgaccgc aatcgtgatg attttgcgct gataacaggc gggtaaaatg
    10321 gggatcaaag taaaaggcat aagacaggtc aagcagcggc tgaatcaggt tgttggcgag
    10381 gttcagagcc aaaaagcaac tcgggcaatg tttcgcattt tgaatgcggt tgcccctctt
    10441 gcggcgcact atacgcctgt tgatacctca acgttgatca acagtcaatt cactgaaatg
    10501 gaagtgaacg gcacacgctt gattggaaga gtgggttatt ctgctaatta tgcggcttac
    10561 gttcacgatc ctaatatcaa gcaaaacttc cgcaagcctt cagcgaagaa agagtttttg
    10621 acttccgcgc tgaacgaatc aaaggctacg atcaaatcca ttgttaaaga ggagttgtca
    10681 ctatgatttc ttatgtcaaa gccttcaaac aatggttaga ggctcactct cttgcagacg
    10741 gctatgttgt gcagctttac cagtgggagg atacgcaaaa agccaagcct gttattgtta
    10801 ttcagccaaa tagtggttcg ccgcaagttt ctgacctgag cagcgaacac tatctgctaa
    10861 ttagccttgt tgcaggtaaa aattcaggtt acacaatcga agagcgagca agggcgatta
    10921 tgtcaaagat tttagttgag cctttcgctt cctttggtta catcgaatca atgggcggat
    10981 tacccgcccc aattttcacc gaagataatc gaatgatttt tcgcttacca cttcggatta
    11041 tttcaacaaa tcaagaggag tagtttatgg gttctccatc tccacaaaat aacgcagaaa
    11101 atctcgtggt ggggcgtgcc gtagttttag agtacggtac gactgatgca aagccacaag
    11161 aaagcgaatg gaaggttgcg ggcgcaatga cgacaaagag ttgggatttt agcccaaatt
    11221 cggttacatc agaggctgat gatgctggcg gcttccctga aaccttaatt acaaactcag
    11281 actttagtat ttctggtgac ggtgagtggc gcaaacgacc aaaatcgaaa gaactaggca
    11341 tcaaagatct agttgtgtta tacgcaaatg cggtaaaaaa tcgcacccaa ccttacattt
    11401 gggttcgttt gaaatacgca ggcttaacaa ttctcggaaa aatggttatc actgcattaa
    11461 gtagcgaagc cccaaccaat gacttagtga aattctctat agagttcaag gttggcgatt
    11521 caagcactat cgaaattgct gcagcgtaaa aaatcccacc ctctataaca agagcggtgg
    11581 gattatcatt atcctaggga tagttgtagg ggctttccta agctagccaa aatacgacca
    11641 atagtgtcga tttttgtaat atggcgaggg gctaaaattc gctgtacttc tggcggacgg
    11701 atattagtta atcgggcaat ttctgccttg cttaccgctt gttcttgcat tgtattatgc
    11761 aaaagcactt ttgcgtagat gctctctggc ataaacacag gcacttcgtc ggtatttact
    11821 gggcgagtga gcggaaagcg tctatcttca tcgaaataaa tttcaacaca agatagtagc
    11881 acgtcttctg ccatttccat tgcttcttca aatgtatcgc cttgggtgat agcttctggc
    11941 aaatcaggaa atgtcactac aaagccccct tcttcagcgg gggtaaataa cgctggataa
    12001 aacatatttt ctccttattc aagggtgtag tgaaagcccc ttaacatcag gggctttata
    12061 ttattttaag cctagttgct ttttaacccc ttccactaag cctgttttta actcttggct
    12121 tgggtgtttg gggaggtggc ttcttttgcc cttgtagtag agtttcaaat gctttgatcc
    12181 attttcaacc tctaccccat tagcctttag ccaccttagg aattcgcttt gcttcactac
    12241 ctcctcctat ttgtttaact tgggttcatt atatgcaaaa atgctaactt tgtcaacaga
    12301 aagttagcaa aaatgctaat ttttttgttg aggtgaaaat ggttagctat ccaattactg
    12361 aaattggtga aatgcttatt gcaaccgatg ggcgagactt tttattcaga ccgagtttca
    12421 aaaatatcta caaaattgga agcccacaag aaattgtaaa aatctatgca ctattgcacg
    12481 gctgtgagat tcgagagata ttgccgcaca ttttaaccaa atcggagtct catcaatctt
    12541 acttttggtc agtgctgaat tcgcctgttt ttggcagaaa aattctgcaa aacgcaatga
    12601 tcgtgatgtc gtgctgttgc gatgatgata tttccctgtt gattggcgag tggttgccgt
    12661 caaagtctgg cgttaggtac aagttgggta aaatgccacc tgagacaatt atcaattttg
    12721 caaaaaattt aatgggtcac ggagtgattg gttgctgcga attgccaaag aaagaacaat
    12781 ctgcaaaaaa tgaattttca gaaagttttg atgctgttga gtacataagt ttagcccgaa
    12841 ctcatttcaa tatgagccgc gaagacgctg aaaatctcac gatgaccgaa ttgcagcaac
    12901 ttctaaaaag tcaaatgcct gccgaaaata atagctatgg acagttcacc gaagaagagt
    12961 acgacagaat tatggcggaa tatgagaaaa gaagagcggc ttaaatagcc gctaacattt
    13021 aaaagaacct acctgataga acgggagtga aatccttgta atgtgctgaa taaaaaagct
    13081 ctcttatgtc attgagtagc tcaaattcat ctttggatct tgcgttgcag attgccacat
    13141 agtcatctac gcaggataaa atacgttcaa tactgacttc ttttatccag catacttctt
    13201 ggttttcatt cagccagttg gcaaagtctt gtttagtgca gtcttttttc ataagttctc
    13261 ctttttgatt atttgcaaaa aaaatcatat caaatcatcc gttgttatct attggcgaaa
    13321 ataatttttg cgacacagat cgcaaaaat
//
LOCUS       DANLYI010000050        12604 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.050, whole genome shotgun sequence.
ACCESSION   DANLYI010000050 DANLYI010000000
VERSION     DANLYI010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 12604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12604
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.050"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            complement(<1..202)
                     /locus_tag="PXD81_001069"
     CDS             complement(<1..202)
                     /locus_tag="PXD81_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDL5537105.1"
                     /translation="MPKSWELSLTARSNVIEILQSVEDYTGHWLSAVSLWEQLQNKLD
                     LIAFMPTIGRRREDGTLETFCRN"
     gene            complement(195..386)
                     /locus_tag="PXD81_001070"
     CDS             complement(195..386)
                     /locus_tag="PXD81_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5537106.1"
                     /translation="MLFKEQGYDEFLAEQIKAGQEAIARGETFTKEQINNELNHLFAD
                     MQKRQQLRENENLFEVNYA"
     gene            complement(676..1266)
                     /gene="yraP"
                     /locus_tag="PXD81_001071"
     CDS             complement(676..1266)
                     /gene="yraP"
                     /locus_tag="PXD81_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divisome-associated lipoprotein YraP"
                     /protein_id="HDL5537107.1"
                     /translation="MNKLANKLLKGSLLVLGFISLQGCITTAVVTSAAVATKVATDPR
                     TTGTQVDDEILEEKVAYNINKDEQIKQEARINVVAYNGKVLLIGQAPSMDVVENAKNL
                     AAGAEGVTEIYNEIRQGEKIGFGQITQDSWITTQVKSKLLVNSEVKATEVKVVTENGE
                     VFLMGKVSQNQADAAAEAARNVGGVTKVVKVFSYAQ"
     gene            complement(1276..1860)
                     /locus_tag="PXD81_001072"
     CDS             complement(1276..1860)
                     /locus_tag="PXD81_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDL5537108.1"
                     /translation="MLNKIQDRFTESIQTQISASELLPAKLSEAANRIVACLLRGDKV
                     IVCGHGRSYGNAELLVSHLLHRYELARPSFAAVQLQLSGVLAGVVAQDNELEAVYKKQ
                     LQAVAKEGDLFIVFSPVGNEECILQAIHTAKNQHLDLDIIALTGSNNDHTLGLLDESD
                     IEISAPSTNQLRVIESHHFYVNLLCELIDHLLFS"
     gene            complement(1866..2237)
                     /locus_tag="PXD81_001073"
     CDS             complement(1866..2237)
                     /locus_tag="PXD81_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HDL5537109.1"
                     /translation="MELFNKLINKREQGSFYEQKARVFLEQQGLRFVAANQAFKGGEL
                     DPIMQDGQTVVFVEVRQRKSNRFGSAVESIDYRKQQKWQNAANMWLLKQNQSLETANC
                     RFDVVAFEGNQTPVWIPNFLG"
     gene            complement(2212..3933)
                     /locus_tag="PXD81_001074"
     CDS             complement(2212..3933)
                     /locus_tag="PXD81_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator"
                     /protein_id="HDL5537110.1"
                     /translation="MATILNHTMKKALVPTAIALFISACTSVNPVTESIKNEAYSSSE
                     FYINKADQTKEAEDKISYQLLAVRKLIDENKEYEAQNTFSEILTAEMNEVQKLEYALV
                     SAQLAALQGKNEQATSQLKAIQDPLLSSAQRLRYIQTQARIAANQKDVIGIVRARSQL
                     NNFYKMNRERQENNDIIWQTLRDANRGMLEKTVPEAGEMELAGWLALINIYNQNVTTP
                     AQMPQAINNWKAQYPSHSAVAVMPTELQGVSNFQQTQLNSVALLLPLSGDAKILGDII
                     KRGFNDAKADDSTAVQTFDTDSSDVNSLISQAKQQGANVIVGPLLKSRVDEMLLSPEI
                     QNVNILALNATENVRNIAQVCYYGLSPESEAQSGAEKIYQDGNSVAIVAAPQDDYGNR
                     SAEAFAKRWRQLTNSDADVRYYNQPLDAVAAIQNAGVSKAALYILGNAEQVLEIKQGI
                     DSSTLKDRLAIYTSSRSNSPNNGIDFYTSMEGVKFSEVPLLADQSSSEYKKAENLANS
                     DFSMMRLYAMGADAWSLATKFNEFRHIPGYKISGLTGELKAGQHCNIERSLSWMQYRN
                     GAIQQAY"
     gene            4007..4879
                     /gene="rsmI"
                     /locus_tag="PXD81_001075"
     CDS             4007..4879
                     /gene="rsmI"
                     /locus_tag="PXD81_001075"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HDL5537111.1"
                     /translation="MKNRQNSSGINPNYGILYIVATPIGNLGDITQRALDTLSSVDLV
                     AAEDTRHSGLLLSHYGIKKPFFALHDHNEQQKAVVLVEKLAKGENIALISDAGTPLIS
                     DPGFHLVRHCRQAGVKVVPIPGACAAVTALCASGIASDRFCFEGFLPAKTKSRCDKLT
                     ELENEPRTLIFYESTHRILDTLADMQTVFGEDRYVVMAREITKTWETIYGDQLGNLIE
                     WLNEDSNRIKGEIVLVVEGKPQSSEEAFSSQAIKLLTLLCQELPLKKAAAIVAETFGY
                     KKNALYQYGLEHFE"
     gene            complement(5124..6290)
                     /locus_tag="PXD81_001076"
     CDS             complement(5124..6290)
                     /locus_tag="PXD81_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL5537112.1"
                     /translation="MTNLLLAIIYLSFISLGLPDAILGSAWPSMHQQLAVPLSYAGVV
                     SMIMSIGTITSSLMSDKLTYHLGTGKVMVISVALTALALWGFSISDAFWILCLWAIPY
                     GLGAGAVDAALNNYVAVHYASRHMSWLHCMWGVGASIGPHILGYALLQGRGWEAGYFT
                     ISMLQIGLTLMLFCSLPFWKLRKQITAEINHSAKPMSLKETLKIPGVKYGILAFFGYC
                     GVEQTAGLWASSYFVMVKDVGLEQAAAFGSIFFIGITVGRGVSGFLTLKFSNRQMIKL
                     GSALILVGIAILCLPLGKYVTLASLIIIGLGCAPIFPCLIHSTPAFFGEDKSQSVIGV
                     QMASAYIGVLLLPPIFGFIANAISIQLLPLYLLLMFAIMAYAYRNLVRETSPSP"
     gene            complement(6442..7956)
                     /gene="putP"
                     /locus_tag="PXD81_001077"
     CDS             complement(6442..7956)
                     /gene="putP"
                     /locus_tag="PXD81_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HDL5537113.1"
                     /translation="MFGLDPTTIMFVVYILGMIAIGFIAYRVTNNLSDYILGGRRLGS
                     FVTALSAGASDMSGWLLMGLPGAVYAGGLIEGWIAIGLTIGAYLNWKLVAGRLRSHTE
                     HSGDALTLPEYFHNRFGDKTRLIKILSAVIFLLFFAIYCASGVVAGARVFENLFGLPY
                     HQAIWYGAVATILYTFIGGFLAVSWTDTIQASLMVFALILTPFFVMYNLGGFDEMQAL
                     IDRAGEMAQKDYNDIFTGTSVLGIISLMAWGLGYFGQPHIVVRFMAAESVKSLENARR
                     ISMTWMIICLLGAIGIGYFGMAYFFGNPEQVGNISKNPEQIFIELAKLLFNPWVAGIL
                     LSAILAAVMSTLSAQLLICSSAITEDLYKGILRPQASDKELVWLSRLMVLAVAALAIY
                     IAQDPDSKVFGLVRNAWAGFGSAFGPVVLLSLFWKRMNGFGAIAGMLTGALIVHFWGD
                     ITLKLALPEIYSMIPGFIAATLMIIVVSLITPMPSKAVLDTFDRANKAYLAEVK"
     gene            complement(8050..8838)
                     /locus_tag="PXD81_001078"
     CDS             complement(8050..8838)
                     /locus_tag="PXD81_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HDL5537114.1"
                     /translation="MNAKKEKESSGNQSLIRGLTLLELLAKFPNGCPLAHLAELSGLN
                     KSTVHRLLQGLQQEGYVRPAPTAGSYRLTTKCLAIGQRALSSINILHIAAPHLEALNL
                     TLGETVNFAMRENDHAILINKLEATTGMMRTRAYIGQQMQLYCSGMGKLFLAYGSADY
                     IPQYWQTKSDIIQKLTVNTITTIDGMQTELETIRRQGFAMDNEENELGVSCIACPIFD
                     HQQKVHYAISVSLSTARLNQLGRENLLPAIQETAEKISAELGGR"
     gene            complement(8835..9413)
                     /locus_tag="PXD81_001079"
     CDS             complement(8835..9413)
                     /locus_tag="PXD81_001079"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HDL5537115.1"
                     /translation="MNQSSLTDLRSQLRQSMRLKRQSLTAGQQTQAAESIIPQALSLI
                     ESYQASHIAFYLPFNGEISPIPLMEQLLQQGKKLYLPLLHPFTSGQLLFVNYNCKTIL
                     KFNRLGIQEPILDVRNILPLQELEMIFTPLVACDKAGNRLGMGGGFYDRTLAQAPHLI
                     SVGLAHECQQVEQLPIESWDMPLDHIILGAAK"
     gene            complement(9415..9606)
                     /gene="ssrS"
                     /locus_tag="PXD81_001080"
     ncRNA           complement(9415..9606)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="PXD81_001080"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(9711..10001)
                     /locus_tag="PXD81_001081"
     CDS             complement(9711..10001)
                     /locus_tag="PXD81_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HDL5537116.1"
                     /translation="MSANNIELQIFGQVLRLQCPVEQQEHLLASAQRLEERVVVLKEQ
                     SGIIQLEKVLAIVALNLNYELEQAQRQNAENKNVLVACIEQLDNSLGKFQSN"
     gene            10080..10637
                     /locus_tag="PXD81_001082"
     CDS             10080..10637
                     /locus_tag="PXD81_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005624207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein"
                     /protein_id="HDL5537117.1"
                     /translation="MAITDSQQFKQLVSQLQIDDTPAEFHGFLCGLIAGGIQDESWKT
                     LTYQFTNDGHAFSIEPLNTLTDFYQQLSESFSEANTLFSLWLPQNEEDGFALADGISE
                     WTNSFLLGLGVAQPKLQQETDEVGEAIDDLDEIAKLGYSDDDNNEELLEAGEEVLEYL
                     RVLALFLHSHFALGVPKTEEKPPLH"
     gene            10735..11799
                     /gene="hemE"
                     /locus_tag="PXD81_001083"
     CDS             10735..11799
                     /gene="hemE"
                     /locus_tag="PXD81_001083"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012262703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HDL5537118.1"
                     /translation="MSELKNDRYLKALLREPVDMTPVWMMRQAGRYLPEYKATRAEAG
                     DFMSLCRNADLACEVTLQPLRRYELDAAILFSDILTVPDAMGLGLSFGAGEGPKFARP
                     VASLNDVNQLPIPDPEDELQYVMNAVRTIRRELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTKIKKMLYAEPHILHKLLDKLADSVILYLNAQIKAGAQAVMVFDTWGGVLAHED
                     YKAFSLHYMHKIVDGLIRENEGRKVPVTLFTKGGGLWLEAIAETGCDAVGLDWTVDIA
                     DARRRIGDKVALQGNMDPSVLYASPERIEQEVKNILAGFGNGSGHVFNLGHGIHLDVP
                     VESPKVFVDAIHNYSKQYHK"
     gene            11946..12536
                     /locus_tag="PXD81_001084"
     CDS             11946..12536
                     /locus_tag="PXD81_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005620054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF416 family protein"
                     /protein_id="HDL5537119.1"
                     /translation="MRNPIHKRMERFEPWQNTTFMACLCERMYPNYQLFCEVTEQPEK
                     AKVFQNILNLVWEYLTIKGAKINFDNQLEKLEEIIPDVNEYAFFGVLPAQEACEALSE
                     LLHSIIAGSTLEQAIRISQISLGTVASYLEMQQDKELNEQELKNSEEIQEELDVQWQI
                     YRLLNECEKRDLDLIFELKDEIRSSGISNIGLNINQ"
BASE COUNT         3715 a         2771 c         2559 g         3559 t
ORIGIN      
        1 aattgcggca gaatgtttct aatgtgccat cttctctacg tcgccctatt gtaggcataa
       61 atgcaatgag atcgagttta ttttgtaatt gttcccaaag tgaaactgcg ctcaaccaat
      121 gccctgtata atcttctacc gactgtaaaa tctcaatgac attagacctt gcggtaagtg
      181 acagctccca agatttaggc ataattaacc tcaaacaaat tttcattttc tcgtaattgc
      241 tgacgtttct gcatatccgc aaataaatga tttaattcgt tattgatttg ttctttggta
      301 aaggtttctc ctcgagctat tgcttcttga ccagctttaa tttgctctgc aagaaactca
      361 tcatatcctt gttctttgaa tagcatattc tgctcctttg atttgtgtct tgattccact
      421 atgttcaaga catagtttaa gtttaaatct acgctcaaag caatgaagag tcaagggaat
      481 ccttatgttg atagggtgtg catatttttc gagtcaaatg aattaaaaat catcattacg
      541 atcacccact catagatttt atcttcctat ctaaggtcat cttcttatat caaacaagcg
      601 gtctaaattt gcaaaaaaat gacaaattta gaccgcttgt gagttacaaa aatataaact
      661 aattgagctt atcaattact gcgcataact aaatactttc accactttgg ttactccacc
      721 tacattgcga gcggcttcgg cggcggcatc agcttggttt tggctgactt tgcccattaa
      781 aaacacttcg ccgttttcgg tgacgacttt cacttcggtg gcttttacct cgctatttac
      841 taacagtttg gatttcactt gggtggtaat ccagctgtct tgtgtaatct gtccaaagcc
      901 gattttttcg ccttggcgga tttcgttgta aatttccgtc acgccctccg ccccagcagc
      961 gaggtttttc gcattttcca ctacgtccat acttggtgct tgcccgatta ataacacttt
     1021 gccgttgtaa gccaccacgt taatgcgggc ttcttgtttg atttgctcgt ctttattaat
     1081 gttgtaagcg actttttctt ctaaaatttc atcatcaact tgcgtgccgg tggtgcgtgg
     1141 gtctgtcgcc actttagttg caaccgccgc agaagtcacc accgccgtgg tgatacaacc
     1201 ttgtaaagag ataaaaccta atactaatag gctgcccttt aatagtttat ttgctaattt
     1261 gttcataatc tctccttacg aaaatagtaa atggtcgata agttcacata ataaattcac
     1321 ataaaaatga tggctttcaa tcaccctcag ttgattggtt gaaggggctg aaatttcaat
     1381 atcactttca tctaataagc cgagtgtgtg gtcgttatta ctgcctgtta aagcaataat
     1441 atctaaatct aaatgttgat ttttcgccgt atgaatagct tgcaatatac attcttcatt
     1501 acccacaggc gaaaaaacaa tgaagagatc accctctttt gcaaccgcct gcaactgttt
     1561 tttataaaca gcctctaact cgttatcttg tgccacaact cctgccaata caccgcttaa
     1621 ttgcagttgt acggcagcaa aactcggtct tgccagttca taacggtgaa gtaagtggct
     1681 gaccaataat tcggcattac cgtaagagcg accgtgtccg cagacgataa ctttatcgcc
     1741 acgcaacaaa caggcaacaa tgcggtttgc cgcttcactt aatttagccg gcaatagctc
     1801 agaggcggaa atttgggttt gaatactttc ggtaaatcta tcttggattt tatttaacat
     1861 tctttctaac ctaaaaagtt gggaatccac acaggcgttt ggtttccttc aaaagcaacc
     1921 acatcaaaac ggcaatttgc agtttctaaa ctttggtttt gtttaagcaa ccacatatta
     1981 gcggcatttt gccatttttg ttgcttgcgg taatcaatac tctcaacggc tgaaccgaag
     2041 cggttgcttt tacgctggcg aacttctaca aacacaacgg tttgtccgtc ttgcataatc
     2101 ggatccagtt cgccaccttt aaatgcttgg ttagcggcaa caaaacgaag cccttgctgt
     2161 tctaaaaaca ctcgggcttt ttgttcataa aacgagcctt gttcccgttt attaataagc
     2221 ttgttgaata gctccattgc gatattgcat ccaagataaa ctacgttcaa tattacaatg
     2281 ttgtccggct tttaactcac cggttaaacc tgaaattttg tagcccggaa tatggcggaa
     2341 ttcattgaat ttggttgcca atgaccacgc atcagccccc attgcgtata aacgcatcat
     2401 tgagaaatcg ctattggcta aattctcggc ttttttatat tcactagaac tttgatcagc
     2461 taacagtggc acttcactaa attttacccc ttccattgaa gtgtaaaaat caatgccgtt
     2521 atttggcgaa ttactgcgag atgaggtata gatcgctaaa cgatctttta aggttgagct
     2581 atcaatacct tgtttaattt ctaatacttg ctcggcattc cccaaaatat ataaagctgc
     2641 tttgctcaca cctgcatttt gaatagcggc aacggcatca agcggttggt tgtagtaacg
     2701 cacatcagca tcgctattgg taagctgacg ccagcgtttg gcaaaggctt ctgccgagcg
     2761 gttgccgtag tcatcttgag gggccgcaac gatagcgaca ctattaccgt cttgataaat
     2821 tttttccgcc cctgattgtg cctcagattc aggagataaa ccgtagtaac acacctgagc
     2881 aatattacgc acattttcag tggcattcag tgccaaaata ttcacgtttt ggatttctgg
     2941 gctaagtagc atttcatcaa cacgagattt gagcaatggc ccgacaatca catttgcccc
     3001 ttgctgtttt gcctgtgaaa ttaaggaatt aacatcagat gaatcggtat caaaagtttg
     3061 cactgcggtt gagtcatcag ccttagcatc attaaaacca cgcttgataa tatcacccaa
     3121 aatttttgca tcaccactta gaggcaatag caatgcgaca ctgtttaact gggtttgttg
     3181 gaaattactc accccttgta attccgttgg cattacggca accgcactat ggctcgggta
     3241 ttgggctttc caattattaa ttgcttgtgg catttgtgcc ggtgtcgtca cattttgatt
     3301 atagatatta attaatgcca accaaccagc aagctccatc tcacccgctt caggcacagt
     3361 tttttccagc attcctcgat ttgcatcacg caaggtttgc caaataatat cgttattttc
     3421 ttggcgttca cggttcattt tatagaaatt gttaagttgc gatcttgcac gcacaatgcc
     3481 aatcacatct ttctgattgg cagcaatacg ggcttgggtt tgaatataac gcaaacgctg
     3541 tgccgaactt aatagcggat cttgaatggc ttttaattgg cttgttgctt gctcgttttt
     3601 cccttgtaag gcggcaagct gggcggaaac caaggcatac tctaactttt gcacctcgtt
     3661 catctctgcg gtcagaattt cactaaaggt attttgagcc tcatattcct tattttcatc
     3721 aattaatttg cgtacagcta aaagttgata cgaaatttta tcttccgctt ctttggtttg
     3781 atctgcttta ttaatataga attccgagct tgaataggct tcatttttaa tggattcggt
     3841 gacaggattt accgatgtac aggcagaaat gaataatgca atcgccgttg gcaccaatgc
     3901 ctttttcatt gtatggttta aaatagttgc cattgtcaga tcctcaatag aatataatgt
     3961 gctaaattta atgtgaaatc ttacttatca aacgagctaa aagcaaatga aaaacagaca
     4021 aaattcatct ggaatcaacc caaattacgg aattttatat attgttgcca cgcctatcgg
     4081 caatttaggc gatattaccc aacgggcgtt agacaccctt tcatctgtcg atttagtcgc
     4141 cgccgaagac actcgccaca gcggtttatt gctgagccac tacggcatta aaaaaccgtt
     4201 tttcgccttg cacgaccata atgaacagca gaaagcggtc gttttagtcg aaaaattagc
     4261 aaaaggggaa aacattgcgt taatttccga tgccggaacg ccactgatta gcgacccggg
     4321 ctttcatctt gtccgtcatt gccgccaagc cggggtaaaa gttgtgccga tccccggggc
     4381 ttgtgccgct gttactgcct tatgtgcttc aggcatagct tcagaccgtt tttgttttga
     4441 agggttctta ccggcaaaaa caaaatcccg ttgcgataaa cttaccgagc ttgaaaacga
     4501 gcctcgcaca ctgatttttt atgaatccac ccaccgtatt ttagacactc ttgccgatat
     4561 gcagactgtt tttggcgaag atcgctatgt ggtaatggcg cgtgaaatca cgaaaacgtg
     4621 ggaaaccatt tatggagatc aactgggtaa cttaattgaa tggctaaatg aggatagcaa
     4681 ccgaattaaa ggcgaaattg ttctggtggt tgaaggtaaa ccgcaaagta gtgaggaagc
     4741 cttttccagc caagcgatca aattactgac attactctgc caagaacttc cgcttaaaaa
     4801 agccgctgcc attgtggcag aaaccttcgg ctataagaaa aatgccctgt atcaatatgg
     4861 attggagcat tttgagtaac agacaagcgg tcagattttg tgagtttttt gcagattggc
     4921 actcccctct ctctgaaaaa tgtttctgaa cgaaacattt cccggtctca atactcgcta
     4981 cgctcgctcc ttcggagccg ttggcaaagc caacgttcaa atgtaaacat ttgtcccata
     5041 aggggagaga gctaaattat tcgcttgtcg acccctctcc actcgtggga gagggacaga
     5101 ttcttcttcg ttcagaagaa aaatcaggga gagggggaag tttctcgcac taaattccga
     5161 taagcatatg ccataatcgc aaacatcaac agcaagtaaa gcggtaataa ttgaatgctg
     5221 atagcattag caataaagcc gaaaatcggt ggcaataaca ggacgccgat ataggcactt
     5281 gccatttgca cgccgatcac cgattgagac ttgtcctcgc cgaaaaacgc aggggtcgaa
     5341 tgaattaagc acgggaaaat cggggcacaa cctagcccga taataattaa acttgccagc
     5401 gtaacatatt tgcctaacgg caagcacagt atcgcaatgc cgactaagat caaggctgaa
     5461 ccaagtttaa tcatttgtct gttgctaaat ttaagcgtca agaagccact tacgcctctg
     5521 ccaacggtaa tgccgataaa gaaaatactg ccgaacgcag ccgcttgttc taagccgaca
     5581 tctttaacca ttacaaaata gctactcgcc cacagtccgg cggtttgttc tacgccacaa
     5641 tagccaaaaa aagctaaaat gccatatttc acgcccggaa tttttagcgt ttcttttaag
     5701 ctcatcggtt ttgccgaatg gttgatttca gcggtaatct gcttgcgtaa tttccaaaaa
     5761 ggcagtgagc aaaacagcat tagcgttagc ccgatttgta acattgaaat ggtgaaatag
     5821 cctgcctccc agcctcgccc ttgcaataag gcgtagccta aaatgtgcgg cccgatagaa
     5881 gctccaacgc cccacataca atgcagccag ctcatatggc ggcttgcgta gtgaactgct
     5941 acatagttat tcaacgccgc atcaaccgct ccagccccta agccgtaagg aattgcccat
     6001 aaacataaga tccagaaagc atcactgatt gaaaagcccc acaatgctaa agccgttaag
     6061 gcaacgctga tgaccattac tttgcctgtg ccgagatggt aggtcagttt gtccgacatt
     6121 aagctagagg taatagtgcc aatcgacata atcatcgaga ccacaccggc ataggaaagc
     6181 ggaacagcaa gctgttggtg cattgacggc cacgccgagc ctaaaattgc atcaggcaac
     6241 ccaaggctga taaaagagag ataaataata gcgagtagga ggttagtcat agcttacaat
     6301 cggtcacaaa aagcatcgct ttttgaacgt tggctctgcc aacgggagcg aagctgtgaa
     6361 caatccttac aaggattgtg aataattttt gataaatttt ttgtaaaaaa atggcttaag
     6421 gagaccttaa gccaaatttt attatttcac ttctgccaaa taggccttat tagcacggtc
     6481 aaaggtgtct aatacggctt tgcttggcat tggcgtgata agtgatacca caatgatcat
     6541 tagggtagca gcaataaagc ccgggatcat cgagtaaatt tccggtaagg cgagcttaag
     6601 tgtaatatcg ccccaaaaat gcacgattaa cgcaccggtc agcattccgg caatcgcccc
     6661 aaaaccgttc atacgtttcc agaatagtga aagcaatacc acaggcccaa atgccgagcc
     6721 gaaacctgcc cacgcattac gcactaagcc aaatactttg ctgtccgggt cttgggcaat
     6781 ataaatagct aatgctgcca ccgcaagcac catcaagcgg cttaaccaaa ccagttcttt
     6841 atcggaggct tgagggcgta aaatgccttt gtataaatct tcggtaatcg cacttgaaca
     6901 aatcagtaat tgagcggaaa gggtactcat taccgcagca agaatggcag ataataaaat
     6961 gccggctacc caagggttaa ataacaattt agctaattca atgaaaattt gttccggatt
     7021 tttgctgata ttgccgactt gctccgggtt gccaaagaaa tatgccatac caaaataacc
     7081 aatgcctatt gcccccagta agcagataat catccaagtc atactgatac ggcgtgcatt
     7141 ttcaagagac ttcacacttt ctgccgccat aaaacgcacc acaatatgcg gttgtccgaa
     7201 ataacccagc ccccacgcca ttaagctgat aatgcctaat acgctggtgc cggtgaaaat
     7261 gtcgttgtag tctttttgtg ccatctcgcc tgcacggtcg attaaggctt gcatttcatc
     7321 aaaaccacct aagttataca tcacaaaaaa cggggtgaga atcagggcaa aaaccattaa
     7381 tgaagcttga atagtatccg tccaacttac tgctaagaaa ccaccaataa aggtgtaaag
     7441 aatggtagca acagccccat accaaattgc ttggtggtaa ggtaagccaa ataagttttc
     7501 aaataccctc gcaccggcca caacgcctga tgcacagtaa attgcaaaaa agagtaaaaa
     7561 aatgaccgct gataaaatct taattaaacg ggttttatcg ccaaaacggt tatgaaaata
     7621 ttcgggtaag gttaaggcat cgccactgtg ttctgtatgc gagcgtaatc tgcctgcgac
     7681 taatttccag tttagataag caccgatagt taaaccgatt gcgatccagc cttcgatcaa
     7741 accgcctgca taaacggctc cgggtaatcc catcagcaac cagccggaca tatctgatgc
     7801 accggcagaa agtgcggtca caaagctacc taaacggcga ccgcctaaaa tatagtctga
     7861 aaggttattg gtaacacgat aggcaataaa gccgatggca atcattccta gaatatagac
     7921 aacaaacata attgttgttg gatcaagacc aaacattatt aatgaatcct caagatttct
     7981 tattgtataa agattaaagt gagtaaaggg taaaagatta cctgaaataa tcaaaatggc
     8041 aacacaaaat taccgtccgc ccagctcagc tgaaattttt tctgctgttt cttgaatagc
     8101 cggtagtaga ttttcccgcc ccagctgatt taaacgagca gttgaaagcg aaacagaaat
     8161 cgcataatgg actttttgtt ggtgatcaaa aatcgggcaa gcaatacaag aaacccctaa
     8221 ttcattttct tcgttatcca ttgcaaaacc ttgacggcga atggtttcca gctcggtttg
     8281 cattccgtca atcgtggtga tggtattgac cgtcagtttt tgaatgatgt ccgatttagt
     8341 ctgccaatat tgtggaatgt aatctgccga accataagca agaaacagtt ttcccattcc
     8401 cgagcaatag agttgcattt gttgcccgat ataggctcgg gtacgcatca ttccggtggt
     8461 agcttctaat ttattgatta aaatagcgtg atcgttttcc cgcatcgcaa aatttaccgt
     8521 ctcgcctaag gttaaattta gggcttctaa atgtggggcg gcaatatgca aaatgttaat
     8581 cgaacttaag gctcgctgcc cgattgccaa acatttggtg gttaaacgat aactgcccgc
     8641 agtcggtgca gggcggacgt agccctcttg ttgtaagcct tgtagcagac ggtgaaccgt
     8701 gcttttgttc agccccgaaa gctcggctaa atgggcaagc ggacagccgt ttgggaattt
     8761 cgctaacaat tccaatagtg ttaagccacg aattaaactt tgatttccac tcgattcctt
     8821 ttctttcttt gcattcattt ggctgctccc aaaataatat gatctaacgg catatcccaa
     8881 ctctcaatcg gtaattgttc gacttgctga cattcgtgtg ccaaaccgac actaatcaga
     8941 tgaggtgctt gagcaagggt tctatcataa aagccacctc ccattcccag ccgatttccg
     9001 gctttatcac aagcaacaag tggggtaaaa atcatctcta attcttgcaa cggaaggata
     9061 tttctaacat ctaatatcgg ctcttgaatc cccaagcggt taaattttag aatagttttg
     9121 caattgtaat ttacaaacaa taattgaccg cttgtaaaag ggtgcaacag cggcagatac
     9181 agctttttgc cctgttgaag taattgttcc attaaaggaa taggagaaat ttcaccgtta
     9241 aaaggcaaat aaaaggcaat atgtgaggct tggtaagatt caatcaatga aagagcttga
     9301 ggaataatgg attcggctgc ttgagtttgc tgaccggcag ttaaactttg ccgtttaagc
     9361 cgcattgatt gccgtaattg gctacgaaga tcggttaaag atgattgatt catataaaga
     9421 cccgagatgc cgctaccagt aatggtcctt gaaccagtcg gttcaaggga gtcaaactta
     9481 actgctttta ggcttcttac tcctaattaa attagggtaa gcttgctcac cagcgttaga
     9541 tgtccaactc aaagtttcaa attatcggct cagggacgta actcgtaacg cacaccccag
     9601 gcaagactta tagtaacgga atttgatgat ttttcctagt atttaatccc acaaaatgca
     9661 aattctcaat ctttttggct ctattcctaa tcaattcgat taaaaattac ctaattggat
     9721 tgaaatttgc caagcgaatt atctaactgc tcaatacaag ccaccaacac atttttattt
     9781 tctgcatttt ggcgttgggc ttgctccagt tcataattta aattcagtgc cacaatcgct
     9841 aacacttttt caagctgaat aatgccggac tgctctttca ataccaccac acgctcttcc
     9901 aaacgctgtg ctgaagccag taaatgctct tgctgttcaa cagggcattg caagcgtaaa
     9961 acctgcccaa aaatctgtag ttcgatatta tttgccgaca taaatacgaa atcctcactt
    10021 aatcgcaaaa tttttgctat attatgccat tctttagcaa aatcacaaat aatcccatta
    10081 tggctatcac agattcccaa caatttaaac aactggtatc acaattacaa attgatgata
    10141 caccggcaga gtttcacggt tttttatgcg gtttaattgc cggcggtatt caagatgagt
    10201 cttggaaaac cctcacctac caatttacca atgacggaca cgcattttcc atcgagccgt
    10261 taaacacttt aaccgatttt tatcagcaat taagtgaaag cttttcagaa gccaacacgc
    10321 tgtttagcct ttggttgcca caaaacgaag aagatggttt tgccttagct gatggtatct
    10381 ccgaatggac aaacagtttc ttacttggac ttggcgttgc tcaacctaaa ttacaacaag
    10441 aaaccgatga ggttggcgag gcaatcgatg atttagatga aatcgccaaa ctaggctaca
    10501 gtgacgatga taataacgag gaactactgg aggctggcga agaagtgttg gaatatctgc
    10561 gagttttagc cttattcttg cacagccatt ttgctttagg cgtgccgaaa accgaagaaa
    10621 agccaccatt acactaatga ataaatacaa gcggttcaat ttcatctcta ttttgcaaat
    10681 atattatgcg gggcgaaaaa tatttcgccc ctaccaaaat caaaggaaaa accaatgtca
    10741 gaattaaaaa acgatcgcta tttaaaagcg ttattgcgtg aacctgtgga tatgaccccg
    10801 gtctggatga tgcgtcaagc cgggcgttat ttgccggaat ataaagccac ccgagcagaa
    10861 gccggcgatt ttatgtcact ctgccgtaac gctgatttag cgtgtgaagt caccttacaa
    10921 ccgctacgcc gttatgaact cgatgcagct atcctatttt ccgatatttt aactgtgcct
    10981 gatgcaatgg gattaggttt aagttttggt gcaggtgaag gccctaaatt tgcccgcccg
    11041 gtggcaagcc tcaatgatgt caatcagcta ccgattcctg atccggaaga cgagttgcaa
    11101 tatgtaatga atgcggtgcg taccattcgc cgagaattga aaggcgaagt gccactaatt
    11161 ggtttttctg gcagcccttg gacactcgcc acctatatgg tggaaggtgg ttcaagcaaa
    11221 gcatttacca aaatcaaaaa aatgctttac gctgagcctc acattctaca caaattgctg
    11281 gacaagttag ctgacagtgt catcttatac ttaaatgccc aaatcaaagc cggtgctcaa
    11341 gcggtgatgg tattcgacac ttggggcggt gtattggctc acgaggatta caaagccttc
    11401 tcattacact atatgcacaa aattgtggac ggcttaattc gagaaaatga aggacgtaaa
    11461 gtgcctgtta ccctattcac caaaggcgga ggcttatggt tagaagcgat tgcagaaacg
    11521 ggctgcgatg ccgttgggct agattggaca gtcgatattg ccgatgctcg ccgccgtatt
    11581 ggcgacaaag ttgcgttgca aggcaatatg gatccaagcg tgctgtatgc aagccctgaa
    11641 cgaatcgaac aagaagtaaa aaacattctt gccggcttcg gcaacggcag cggacacgtc
    11701 tttaatcttg gacacggcat tcatttagat gtgccggtgg aaagcccgaa agtgtttgtt
    11761 gatgcgattc acaactactc gaagcagtat cataaataat ggataaaatg ccctctctcc
    11821 ttgtgggaga gggacagatt tttctttgtt cagaagaaaa atcagggaga ggggcaagcg
    11881 gtcattttta tacagaattt tgcaaaatcg ttcccttctc tctgaaaaat aaagaaggaa
    11941 atacaatgag aaacccgatt cataaacgca tggaacgttt cgagccttgg caaaacacca
    12001 catttatggc gtgcctatgc gaaagaatgt accccaacta tcagcttttt tgtgaagtaa
    12061 ccgaacagcc tgaaaaagct aaggtattcc aaaatatttt aaatttagtc tgggaatatt
    12121 taaccataaa aggggcaaaa attaatttcg ataaccaact ggaaaaatta gaagaaatca
    12181 ttcctgatgt gaacgaatat gcgttttttg gtgtcttacc tgcacaggaa gcttgcgaag
    12241 ccctttctga acttcttcac agtattatcg ctggttcaac ccttgaacaa gcaatccgca
    12301 tcagccaaat ttcattaggc acagttgcca gctatttgga aatgcaacaa gataaagaac
    12361 tgaacgagca agagctaaag aattctgaag aaattcaaga agaactggac gtacagtggc
    12421 aaatataccg cttgctaaac gaatgtgaaa aacgtgatct tgatctcatt tttgaactta
    12481 aagatgaaat cagaagcagc gggattagta atatcgggtt aaatattaac caatagcatt
    12541 gatatttgca aaatcttgct taataatact ttgagaaatc ttaactttga attaagctat
    12601 cgca
//
LOCUS       DANLYI010000051        12599 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.051, whole genome shotgun sequence.
ACCESSION   DANLYI010000051 DANLYI010000000
VERSION     DANLYI010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 12599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12599
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.051"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            <1..873
                     /locus_tag="PXD81_001085"
     CDS             <1..873
                     /locus_tag="PXD81_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
                     /protein_id="HDL5537120.1"
                     /translation="KTVFDNEHLLGIDLQVYGAEYWNVSIDNKPSLDWVSAEIAIENE
                     KLGILSWKPSLVCPGERLIQNVSEMIGRSLYVVQMQKQQQQLVLMEERAIIARELHDS
                     LAQSLTFFKIQVSLLKLAKTDEKRNEILTVFEKALNDAYSQLRELLTTFRLTIQEANL
                     QQALEKILESLRPKTSAQIRLSCKLPSHMFNAQQQVHVLQIVREAVINAIKHSQATEI
                     NVIAETKDDGEHCLTIQDNGVGLAAHTQPEGHYGLTIMQERAAELKAEFSIENVESGG
                     VKVQVLLPSMISKR"
     gene            924..1442
                     /locus_tag="PXD81_001086"
     CDS             924..1442
                     /locus_tag="PXD81_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDL5537121.1"
                     /translation="MQFRKAVEADFETILAIYNQAIPTHQITADLELATTQNRRKWFD
                     FHLETAQYPIWVVEDEKGIAGWFSFSPFYERPAFLHTSEISIYLDRYSKGKGYGSKII
                     EFMQAEMLNHNIHTLMAYVFELNTVSQNLMRKHGFEQWGRFPNIANMGKDEKGEDKWR
                     TLLMMSYQKGIE"
     gene            1485..2084
                     /gene="rdgB"
                     /locus_tag="PXD81_001087"
     CDS             1485..2084
                     /gene="rdgB"
                     /locus_tag="PXD81_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HDL5537122.1"
                     /translation="MEKTKIVLATSNADKVKEMADVLSQFGFEVVAQSEFGIESPEET
                     GLTFVENALIKARFAAKMTGLPAIADDSGLSVMALGGEPGLYSARYAGEQATDADNRQ
                     KLLAKMQGQENRLAKFVSCIVFLKHETDPTPYIASGECFGEILTEERGENGFGYDSLF
                     FYPPKACTFAELETKEKKAISHRAIALEQLKQQLAGENK"
     gene            2081..2401
                     /locus_tag="PXD81_001088"
     CDS             2081..2401
                     /locus_tag="PXD81_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005600559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjQ family protein"
                     /protein_id="HDL5537123.1"
                     /translation="MIITTTPTIEGKQIVEYKGLVFGEVVSGANFIRDFLAGITDIIG
                     GRSGAYESKLNGARKEALAELAKEAQKVGANAVVGVSMEYQSMGGDKGMFIVVATGTA
                     VVIR"
     gene            2405..2857
                     /locus_tag="PXD81_001089"
     CDS             2405..2857
                     /locus_tag="PXD81_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HDL5537124.1"
                     /translation="MQFEQHFEANFWQNKSLLEMNDAEWEALCDGCGKCCYRKYIQGR
                     GRRERLYYTSVACNLLDVNTGRCMNYSNRFKIESDCTKLTKKNLPDFGWLPKTCAYRL
                     LYEGKPLFDWHPLISKDPDSVKNAGMLIANGIHEKDVIDWFEFVIDEV"
     gene            complement(2898..3479)
                     /gene="lpcA"
                     /locus_tag="PXD81_001090"
     CDS             complement(2898..3479)
                     /gene="lpcA"
                     /locus_tag="PXD81_001090"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HDL5537125.1"
                     /translation="MYLEQIKAELQEAADVLDKFMSDEKNIKLIQDAAMLIANSFKQG
                     GKVISCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHLSCVSNDFGYEYVFS
                     RYLEAVGQKGDVLFGLSTSGNSKNVLNAIKVAKEKGMKVIAMTGKDGGQMAGLADVEI
                     RVPHFRYADRIQEIHIKVIHILMMLIEFEMEKA"
     gene            3788..4747
                     /locus_tag="PXD81_001091"
     CDS             3788..4747
                     /locus_tag="PXD81_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDL5537126.1"
                     /translation="MVVNPESFEQIIGQSVKIKEVVEQAKKFANLDAPLLIQGETGTG
                     KDLFAKSCHHFGSRRMQKFIAVNCAGLPAEEAESEMFGHRGNGVESVGFFEYAHGGTV
                     LLDSISELSLEMQAKLLRFLNDGSFRRVGEDQEIQVNVRVICTSLQPLSLLVEKGKVR
                     EDLFHRLNVLTLNLPPLRDRKEDIPLLADYFIAQISNQLGVSKLEYDNVFLQALQNYH
                     WRGNLRELYNALYRACTLSHSYRLAVKDLHLPRDMEAMIEVREEATLEEMMSQYEAQL
                     LHQFYAEYPSTRKLAQRLGLSHTAVANKLRQYGLTKNGNSKDE"
     gene            4740..6491
                     /locus_tag="PXD81_001092"
     CDS             4740..6491
                     /locus_tag="PXD81_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL5537127.1"
                     /translation="MNKLAVIFSVCFANLGLNSTAYAAPSIPQELLENSLVYCTTSSG
                     FSFNPQKSDVSSNMNVVTEQIYDKLVEFNPKKNRLEPALAESYSISDDGLVITLRLRK
                     NVAFHTTPWFTPSRKLNAEDVVFSLKRMIGKESDLPEVKSEPESVLHHQRQSYIYQKT
                     AGQIYFPYFESVDLANKIASIHSPSNDIVKIELVRPDSALLAHLASQYAVILSKEYAL
                     QLNADDNLVQLDLLPVGTGVYQLESYENNDYVRLKPNMNYWGKKAYIQNMVVDFSTEA
                     TGRMAKFLNNECDVVAFPEPSQLSVLNNRQLISSKGANLVYLAFNMQRGIGQDLALRQ
                     RIARGINRKRIAEKLFYGTGEVADNLLPTALWSKKNNGSYPYQPHQNSGEQAVEMHDS
                     IINRSLDSKTETEEVNQKQRLKLWVIDEKRVYNQHPLKMAEMIRADLATQNLEVEIRA
                     VSRAYLVQQLANKSADYDLILGGWLANNLDPNSFLEALLSCKAAETVTNLSNWCNEDF
                     DFWLQTARLSDDPASANLLYEFAQKLLEEQLPILPLMNANRVLVIKDGVKQADISPFG
                     QVKLSELRLGLGHKKGN"
     gene            6494..7459
                     /locus_tag="PXD81_001093"
     CDS             6494..7459
                     /locus_tag="PXD81_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HDL5537128.1"
                     /translation="MLIVFFRKIFLTLITLIFLSLISFNILLRDPLNHLAELGWFEAY
                     CYYVKSLLNGDFGISFSNGEPLTAQILQVFPATITLCLSALIVSLIIGLPLGFFAATQ
                     QKNMVGRLLNTLGSLSLAVPVFWLALVLMYYASLNQWEVSAIGEIHSLYPKQMVTGFL
                     LLDIWLSDVPYKLKMMQSALHHLALPTLVLAVPATLEVMHITQARAGYVMKQNYIKIA
                     KTRGWTPFRVWRTHIICNTLPALLPMIARTFILIFAFGMLIENIFSWGGIGRWLINAL
                     AIQDYNAISAGVVAIGVFVLLVDMVTGFVRVMLDPSDKKDWYGSY"
     gene            7446..8339
                     /locus_tag="PXD81_001094"
     CDS             7446..8339
                     /locus_tag="PXD81_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HDL5537129.1"
                     /translation="MAVIKEPEQFRETNAFKAFWFHFRQDKLALGSFYLFLLLLALIF
                     VGSLLAPYNYNEQFVGLELMPPSWDDKGQISHFLGTDDLGRDILSRIMYGFYYTLGSA
                     LMITLLIAIIGGMIGIYAGLKKGRSFWLISHLFDTFLFMPILLIAIIIATLMEASLVN
                     AMLAIFLAMLPHFIHKIYQATEKQLKKEYIITLKLDGASCWYLIREVIIPNLTGVAIK
                     ALSHIFVIAILDISALSFIMLGAKSPTPEWGAMIRESMELIYIAPWTVILPGLAIIFS
                     IFTVTILGTRLSVIFEKYRKE"
     gene            8410..9462
                     /locus_tag="PXD81_001095"
     CDS             8410..9462
                     /locus_tag="PXD81_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL5537130.1"
                     /translation="MALLDIRHLSIEIDMPKGRVKMVDNINLTVDEGEICGLVGESGS
                     GKSLIAKVICGMIKDEWIVSADRFRFDDIELLKLSPAERRKLVGDHISMVFQDPLTSL
                     DPSQQIGKQLMQTIQFRGKWWQRFGWKKKKAIELLHRVGIRDHQDIMKSYPSDITEGE
                     AQKVMIAMAVANQPRLLVADEPTNIMESTTQLQVYRLLSSMNQNLGTSILLVSNDMRS
                     IQNWVDSYNVLYCGQTVEIADKESILESSYHPYTRVLLNSSPDFSQPLAFKSSLNTLR
                     GSVPMLQEMPIGCRLGPRCPFAQKKCINKPPLVKVKQHEFACHFPINFREQNLLKNKT
                     ENTPFVVAEIAEKEAF"
     gene            complement(9471..9701)
                     /locus_tag="PXD81_001096"
     CDS             complement(9471..9701)
                     /locus_tag="PXD81_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HDL5537131.1"
                     /translation="MKLTACKLVFLLYLMFNLTACGTVLSLVEQDYRVYAGVIKDFQV
                     MQEGGIFAVLAVIDLPLSFVLDTLMLPVTLSQ"
     gene            9831..10913
                     /gene="leuB"
                     /locus_tag="PXD81_001097"
     CDS             9831..10913
                     /gene="leuB"
                     /locus_tag="PXD81_001097"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HDL5537132.1"
                     /translation="MQTYNIAVLAGDGIGPEVMAEAIKVLEATQQKFGFKLNFNQYNI
                     GGAAIDAQGKALPENTLKGCEEADAILFGSVGGPKWTHLLPDEQPERGSLLPLRKHFS
                     LFCNLRPAALYQGLEKFCPLRADIAAKGFDMVVVRELTGGIYFGQPKGRDGEGAQTRA
                     FDTEVYYKYEIERITRVAFESAMKRKKHVTSVDKANVLQSSILWRETVTEVAKEFPEV
                     TLEHMYIDNATMQLIKAPESFDILLCSNIFGDIISDEAAMITGSMGMLPSASLNEQGF
                     GLYEPAGGSAPDIAGKNIANPIAQILSAAMMLRYSFNLGDAADAIETAIQKALADGYR
                     TADLADDSKPLSTSEMGDIIAKNILA"
     gene            complement(11000..11395)
                     /gene="msrB"
                     /locus_tag="PXD81_001098"
     CDS             complement(11000..11395)
                     /gene="msrB"
                     /locus_tag="PXD81_001098"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012262815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HDL5537133.1"
                     /translation="MIKPIHALTEQQVDILINHGTERPFTGKFLNEHRVGIYRCARCH
                     NDLFHSDTKFDAGCGWPSFYQTISESSLRYLDDYSLGRHRIEIRCGNCDSHMGHVFPD
                     GPPPTGLRFCLNSAALNFKWEETGEEIDG"
     gene            11620..>12599
                     /gene="gap"
                     /locus_tag="PXD81_001099"
     CDS             11620..>12599
                     /gene="gap"
                     /locus_tag="PXD81_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HDL5537134.1"
                     /translation="MAIKIGINGFGRIGRIVFRAAQHRDDIEVVGINDLIDVDYMAYM
                     LKYDSTHGRFDGTVEVKDGQLVVNGKAIRVTAERDPANLKWNEIGVDIAVEATGLFLD
                     DETARKHITAGAKKVVLTGPSKDATPMFVNGVNFDTYAGQDIVSNASCTTNCLAPLAK
                     VIHEKFGIKEGLMTTVHATTATQKTVDGPSAKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPALNGKLTGMAFRVPTTNVSVVDLTVNLEKPATYAEICAEIKRASENEMKGVLGYTE
                     DAVVSTDFNGATETSVFDAAAGIALTDTFVKLVSWYDNEVGYSNKVLDLVA"
BASE COUNT         3905 a         2209 c         2723 g         3762 t
ORIGIN      
        1 aaaacggtat ttgataatga acatttgcta gggattgatt tacaagtata tggggctgaa
       61 tattggaatg tatcaataga caataaacca agtttagatt gggtttctgc tgaaattgca
      121 atagaaaatg aaaaattggg tatcttaagc tggaaaccct cactggtttg tcctggtgaa
      181 cgattgatcc aaaatgtgtc ggaaatgatt ggtcgtagcc tttatgtagt gcagatgcaa
      241 aagcaacagc aacaattggt actcatggag gagcgtgcaa ttattgctcg tgagttacac
      301 gattccttag cccaatctct tacgtttttc aaaattcagg ttagcctgct taagcttgct
      361 aaaacagatg aaaagcgtaa tgaaatttta actgtatttg aaaaagcgtt aaatgatgct
      421 tacagccaat taagggagct gttgacgacg tttaggctaa cgattcaaga agcgaattta
      481 caacaagcgt tagaaaaaat tttagaaagc ttaagaccga aaacctcagc acagattcgc
      541 ttaagttgca aattaccttc gcatatgttt aatgctcagc agcaagttca cgtattgcaa
      601 attgtacgag aagctgtgat taatgctatt aagcattcgc aagccactga aattaatgtg
      661 attgctgaaa caaaggatga tggtgaacat tgtttgacta ttcaagataa tggcgtaggg
      721 cttgctgccc atacgcaacc agaagggcat tatggactga cgattatgca agaacgggcg
      781 gctgaactca aagccgaatt ttcgattgaa aatgtggaaa gtggcggagt aaaagtacag
      841 gttttattgc cgagtatgat tagcaagcgg taataaaata ttaatttttt gcgtattatt
      901 ggcaacgaat aaaaggataa caaatgcaat tccgaaaagc agtagaagcg gattttgaga
      961 caatattggc aatttataat caagcaattc ccactcacca aattaccgcc gatttagaac
     1021 tggcaactac gcaaaatcgg cggaagtggt tcgattttca ccttgaaaca gcccaatatc
     1081 ctatttgggt ggtcgaagat gaaaaaggca ttgctggctg gtttagtttt tcaccatttt
     1141 atgaaagacc ggcattttta catacttcgg aaatcagtat ctatttagac cgctattcca
     1201 aaggaaaagg ctatggctca aaaattattg agtttatgca agctgaaatg cttaatcaca
     1261 atattcatac gttgatggcg tatgtgtttg aattaaatac agtgagccaa aatctaatga
     1321 gaaaacacgg ctttgaacaa tggggaagat ttccgaatat tgctaatatg ggaaaagatg
     1381 agaaaggtga ggataaatgg cgaacgttgc tgatgatgtc ttaccaaaaa ggtatcgaat
     1441 aacaagcggt gattttttag aatatttttg caaataagga caatatggaa aagacaaaaa
     1501 ttgtacttgc aacaagtaat gcagataaag ttaaagaaat ggcagatgta ctctcacaat
     1561 ttggttttga ggtggtggct caaagtgaat ttgggattga atcaccggaa gaaaccggtt
     1621 tgacttttgt tgaaaatgcg ctgattaaag cacgatttgc tgctaaaatg acaggtttac
     1681 cggcgattgc cgatgattca ggtttatcgg ttatggcact tggtggtgaa ccggggctat
     1741 attctgcacg ttatgccgga gagcaggcaa ccgatgccga taatcgccaa aaattactag
     1801 ccaaaatgca agggcaagag aatcgtctgg cgaaatttgt cagttgtatc gtgtttttaa
     1861 aacacgaaac tgacccaaca ccttatattg cgtcgggtga atgtttcggt gaaattttga
     1921 cggaagaaag aggtgaaaat ggtttcggct acgattccct gtttttctac ccaccgaaag
     1981 cttgcacttt tgccgaatta gaaaccaaag agaaaaaagc gatttcacac cgtgcaatcg
     2041 cactagagca attaaaacag caattagcag gagagaataa atgattatta caactacacc
     2101 tacgatagag ggtaaacaaa ttgtagaata caaagggtta gtatttggcg aagtagtttc
     2161 tggggctaat tttatccgtg atttcttagc cggtatcacc gatattattg gcggacgttc
     2221 aggtgcttat gaaagcaaat taaacggtgc aagaaaagag gcattagctg aattagctaa
     2281 ggaggcacaa aaagtggggg caaatgcggt ggttggcgtt tctatggagt atcaatcaat
     2341 gggcggcgat aaaggaatgt tcatcgtggt agcgactggt actgcggttg taatacgtta
     2401 atttatgcag tttgaacaac actttgaagc aaatttctgg cagaacaaat cgctacttga
     2461 aatgaatgat gctgaatggg aagcattatg tgacggttgt ggtaagtgct gctaccggaa
     2521 atatattcag ggaagagggc ggcgtgaacg gctttactat acctcggttg cctgtaattt
     2581 attagatgtg aatacaggcc gttgtatgaa ttactcgaac cgttttaaaa ttgagtcgga
     2641 ttgtacaaaa ttaactaaaa agaacttacc tgattttggc tggttgccaa aaacctgtgc
     2701 ttatcgttta ctttatgaag gaaagcctct ttttgattgg caccctttaa tctctaaaga
     2761 tcctgattct gtaaaaaatg ccggaatgct gattgctaac ggtattcacg aaaaagatgt
     2821 gattgattgg tttgagtttg ttattgatga agtatgagaa aaaggcgttt ggtgcaaacg
     2881 cctttttgtt atagagatta ggctttttcc atctcaaatt caattaacat catcaaaata
     2941 tgaatcactt taatgtgaat ttcttggatg cggtctgcgt aacggaaatg aggtactcgg
     3001 atttccacat cggctaatcc tgccatttgc ccgccatctt tgccggtcat tgcaatcact
     3061 ttcatgcctt tttctttggc aactttgatc gcatttaaca catttttaga gttaccagag
     3121 gttgatagac caaagagtac atcgcctttt tgcccaacgg cctctagata acgagagaag
     3181 acatattcat aaccgaagtc attacttaca cagcttaaat ggctgacatc ggaaatagcg
     3241 attgccgggt atccgggacg gttttcacgg taacgacctg ttaattcttc ggcaaagtgc
     3301 atcgcatcac agtgcgaacc gccattaccg caagaaatca ccttaccacc ttgcttgaaa
     3361 ctatttgcaa tcagcatggc ggcatcttga attaatttaa tatttttttc atcagacata
     3421 aatttgtcta atacatctgc agcttcttga agctcagctt taatttgttc taaatacatc
     3481 atttgctcct ttgtttgcaa aacgcttacc tttaaacatc ggctttacca atgggggtag
     3541 cgagtaaaaa attcgttttt tatgaccgct tgaggcataa aaagtgcaat aagtttagaa
     3601 gatctatgct gtattgtaaa cctaataact taaataatct gaatagggtt gtaaacaaaa
     3661 gtttccaaaa gagaaaaata gcggtatgat cgcctcacaa tccctctcgc attgttaact
     3721 ctagtagcta atatcaggct ttaagcgtat taaacaatgc acaaatttgc tatttatagg
     3781 agactatatg gtcgtaaatc cagaatcttt tgaacagata attggacaat ctgtaaagat
     3841 aaaagaggtt gttgaacaag caaaaaaatt tgccaacctt gatgcaccat tattgattca
     3901 aggtgagacg ggaacaggaa aagatttatt tgccaaatct tgtcaccatt ttggatctcg
     3961 tcgaatgcaa aaatttattg ccgtgaactg tgccggttta ccggctgaag aagcagaatc
     4021 ggaaatgttc ggacatcgtg gcaatggcgt tgagtccgtc ggtttctttg aatatgctca
     4081 cggtggtaca gtattattag actctatttc tgagttatcg ctggaaatgc aagccaaatt
     4141 attgcgcttt ttaaatgatg gctcttttcg tcgagtgggc gaagatcaag agattcaagt
     4201 caatgttagg gtgatctgta cttcattaca gcctttgtca ttattagttg aaaaaggcaa
     4261 agtaagagaa gatctgtttc atcgtttaaa tgttctcact ttaaatttac cacctttgcg
     4321 agatcgaaaa gaggatattc cattattagc tgattacttt atcgcccaaa ttagcaatca
     4381 gttgggggtg agcaaattag agtatgataa tgtttttttg caagcattgc agaattatca
     4441 ctggcgaggc aacttaagag agctttataa cgcactttat cgagcttgta cactttcgca
     4501 tagctaccgt ttagcggtaa aagatcttca tttacccaga gatatggagg cgatgattga
     4561 agtacgtgag gaggcgacac ttgaggaaat gatgagccag tatgaagccc aattattgca
     4621 ccaattttat gcagaatatc cgagtacccg aaaattagca caacggctcg gactttcaca
     4681 tactgcggtg gcaaacaaat tgcgtcaata tgggttaaca aaaaatggaa acagtaaaga
     4741 tgaataaact agcggtcatt ttttcagtat gttttgcaaa tttagggcta aattcgaccg
     4801 cttacgcagc gccaagtatt cctcaagagc tacttgaaaa tagccttgtg tattgcacta
     4861 cttcatcggg attctctttt aacccacaaa aatccgatgt gagttcgaat atgaatgtgg
     4921 taacagagca gatctacgat aaattagttg agtttaatcc gaaaaaaaat cgactagagc
     4981 ctgctttagc tgaaagttat tcaatcagcg acgatggttt agtgattaca ttacgtttaa
     5041 gaaaaaatgt cgcttttcat acaactccat ggtttactcc gagtcgtaaa ttaaacgcag
     5101 aagatgtggt gttttcgcta aaacgaatga ttggtaaaga aagtgattta cctgaagtga
     5161 aaagtgagcc tgaaagtgta ttacaccacc agcgacaaag ctatatttac caaaaaacag
     5221 cggggcagat ttattttcct tattttgaaa gtgttgattt agcgaataaa attgccagca
     5281 ttcattctcc gtcaaatgac attgtaaaaa ttgaattagt ccgtcctgat tcagcattgt
     5341 tggcgcattt agccagccaa tatgcagtta ttctgtctaa agaatatgct ttacagctca
     5401 atgcagatga taacttagtt caactggatt tattaccggt aggaacaggc gtatatcaac
     5461 ttgaaagtta tgaaaataat gattatgtgc gtttaaaacc aaatatgaat tactggggca
     5521 aaaaagcata tattcaaaat atggtcgtag atttttccac agaagcgacc ggcaggatgg
     5581 caaaattttt aaataatgaa tgtgatgtgg ttgcttttcc ggaacctagc caattatcag
     5641 tgttaaataa tcggcagctt attagtagta aaggtgctaa tttggtttat ttagcattca
     5701 atatgcaacg gggtattggg caggatttgg cattacgcca acgtattgca cgcggcataa
     5761 atcgcaaacg tattgcagag aaattatttt atggtacagg tgaagtagcg gataacctat
     5821 taccaacagc tttgtggtcg aaaaaaaata atggaagcta cccttatcaa ccacaccaaa
     5881 attcaggtga gcaagcggtt gaaatgcatg acagtattat aaatcggtct ttagatagca
     5941 aaacagagac cgaagaagta aaccaaaaac agcgcttaaa actgtgggta attgatgaaa
     6001 aacgagttta taatcaacac ccattaaaaa tggcagaaat gatacgtgct gatttagcta
     6061 cgcaaaattt agaggtcgaa attcgcgcag taagtcgagc ttatttggtg caacagcttg
     6121 ctaataaaag tgcggattat gatttgattt taggtggttg gttagcaaat aatcttgatc
     6181 ctaacagttt tttggaggcc ttactcagtt gtaaagctgc ggaaacggtg actaatttat
     6241 caaattggtg caatgaagat tttgattttt ggttacaaac agcccgtcta agtgatgatc
     6301 cggcatcagc taatttattg tatgaatttg cacaaaaatt attagaagag caattgccta
     6361 ttctcccttt aatgaatgct aatcgtgtat tagtcataaa agatggagtg aaacaggcag
     6421 atatttcccc tttcggccag gtaaaattat cagagttacg tttgggcttg gggcataaaa
     6481 aaggaaacta attatgctga ttgtcttttt tcgaaaaatt tttctaacct tgattacgtt
     6541 gatttttttg tcacttatca gttttaatat tctgttacga gatccattaa accacttagc
     6601 cgagttaggc tggtttgagg cttattgtta ttatgtaaaa tctctgctaa atggtgattt
     6661 tggtattagt ttcagtaatg gagaaccgct cacagcgcaa attttacagg tgttcccggc
     6721 tacaattacg ctttgtttat cggctctcat tgtttcttta attattggtt tgcctcttgg
     6781 tttttttgcg gctactcagc aaaaaaatat ggttgggcga ttattaaata cactaggttc
     6841 attgagttta gccgtgccgg tattttggct ggcattagtg ctaatgtatt acgcttctct
     6901 caatcagtgg gaagtgtcag caattggaga aatccattcg ctttatccga aacagatggt
     6961 aaccggcttt ttgctattag atatttggtt atctgatgta ccttataaat taaaaatgat
     7021 gcagagtgct ttacatcact tagctttgcc gacattagtc ttggctgtgc cagctacatt
     7081 agaagttatg catattactc aagctagagc aggttatgta atgaaacaaa attacattaa
     7141 aatagcgaaa acgcgtggct ggacaccgtt tagagtttgg agaacgcata ttatttgtaa
     7201 tactttacct gcattattac ctatgattgc ccgtaccttt attttaattt ttgccttcgg
     7261 tatgttaatt gagaacatat ttagctgggg aggaattgga cgttggctga taaatgccct
     7321 tgctattcaa gactataatg cgatttcagc gggagtagtt gcaatcgggg tgtttgtgtt
     7381 attagtagat atggttacag gctttgtgcg ggtgatgtta gacccatcag ataaaaagga
     7441 ttggtatggc agttattaaa gaacccgagc aatttagaga aaccaacgca tttaaggcat
     7501 tttggtttca tttccgacaa gataaacttg cacttggcag tttttatctt tttttgctat
     7561 tactggctct catttttgtt ggtagtttgc ttgctcctta caattataat gaacaatttg
     7621 ttggtttaga attaatgcct ccctcttggg acgacaaagg tcaaatcagc cattttttag
     7681 gcactgacga tttaggacgg gatattttaa gccgtattat gtatggtttt tactacacgc
     7741 ttggttccgc actgatgatt accttattaa ttgcgattat cggcggtatg atcgggattt
     7801 atgccgggct gaaaaaagga cgttcatttt ggttgattag ccatttgttc gatacctttt
     7861 tatttatgcc gattttattg attgcgatca tcattgcaac cttaatggaa gcgagtttgg
     7921 tcaatgcaat gttggcgatt ttcttagcga tgttgccgca ttttatccat aaaatttatc
     7981 aagcaaccga aaaacaactc aaaaaagagt acatcattac cttaaagcta gacggtgcaa
     8041 gctgctggta tttaattcga gaagtgatta ttcctaatct taccggcgtg gcaattaaag
     8101 cactttcaca tatttttgtg atcgccatat tagatattag tgcgttaagt tttattatgc
     8161 tgggggctaa aagcccgacc cctgaatggg gagcgatgat tcgtgaatcc atggaactga
     8221 tttatatagc tccttggacg gtgatattac ctggcttagc aattattttt agtattttta
     8281 ccgttacaat acttggtaca cgcttaagcg tgatttttga aaaataccgt aaagaatagt
     8341 atttaaatag gatactgaca cataacaagc ggtcattttt aagtaaaatt ttacagatta
     8401 aggaatagta tggcattact tgatattcgc catttaagta ttgaaattga tatgccaaaa
     8461 ggcagagtaa aaatggtgga taacatcaac ctgactgttg atgagggcga aatttgtggc
     8521 ttggtgggag aatctggttc gggaaaaagc ctcatagcca aagtgatttg cggtatgatt
     8581 aaagatgaat ggattgtgag tgcagatcgc tttcgctttg acgatattga attactgaaa
     8641 ctttcgccag cggaacgccg aaaattggta ggcgatcata tttcaatggt attccaagat
     8701 ccgcttacta gccttgaccc aagccagcaa attggtaaac aactgatgca aaccattcaa
     8761 tttagaggga agtggtggca acgatttggg tggaaaaaga aaaaagcaat cgagctacta
     8821 caccgtgttg gtattcgtga tcatcaagat attatgaaaa gttacccaag cgatattacc
     8881 gaaggcgaag ctcagaaagt gatgattgca atggcggtgg caaatcagcc ccgattattg
     8941 gtggcagatg agccgactaa tattatggaa tcaaccacgc agttgcaggt ttatcgcttg
     9001 ctttccagta tgaatcagaa tttgggaacc tcaatcttgc tggtgagtaa tgacatgcgt
     9061 agcattcaaa attgggttga ttcttacaat gtgctttatt gtggacaaac cgttgaaatt
     9121 gcggataaag agagcattct tgaatcgtct taccacccct atactcgagt gttactcaat
     9181 agttcgccgg acttttctca gcctttagcc tttaaaagta gcttaaatac gctacgaggc
     9241 tcagtcccga tgttacaaga aatgccgatt ggttgtcgtt taggccctcg ttgccctttt
     9301 gcacaaaaaa aatgtatcaa caaaccgcca ttagtgaaag taaaacagca cgagtttgcc
     9361 tgccattttc cgattaactt tagagaacaa aatttgctta aaaataaaac ggaaaatacg
     9421 ccgtttgtgg tggctgaaat agcggaaaaa gaggcgtttt aatgtggtta ttattggcta
     9481 agtgtaacgg gtaacattaa agtatctaat acgaaactca agggtaaatc gatcaccgca
     9541 agcaccgcga atataccgcc ttcttgcatt acttggaaat cttttattac gccggcataa
     9601 acacggtaat cctgttcaac caagcttagt actgtgccac aagcggtgag gttaaacatc
     9661 aaatagagta gaaaaacgag tttacaagcg gtcaatttca tcaaaaaatc tccaaatacg
     9721 cttttatttt acgctatttt tatcgggttg gcgtaaaatt cgttttaaaa acagctattt
     9781 tcaggcagaa tgtagccgaa tcgacattaa atatactaag aggatcttat atgcaaactt
     9841 acaacattgc agtgcttgcc ggtgacggca ttgggccgga agtgatggca gaagctatca
     9901 aagtgcttga agcgactcaa caaaaatttg gctttaaact aaattttaat caatataaca
     9961 ttggtggagc agcgattgat gctcaaggta aagcgttacc cgaaaatacc ttaaaaggct
    10021 gtgaagaggc agatgcgatt ttgtttggct cggtaggtgg cccgaaatgg acgcatttac
    10081 tacctgatga gcaacctgaa cgaggttcgc tattaccact gcgtaaacat ttctcgctat
    10141 tctgtaattt acgccctgct gcattgtatc aagggctgga aaaattctgc ccattacgtg
    10201 ccgatattgc tgctaaaggt tttgatatgg tggtggtgcg tgaattaacc ggcgggattt
    10261 attttggaca gccgaaaggc cgtgatggtg aaggtgcgca aacgagagcg tttgacaccg
    10321 aagtatacta caaatatgaa atcgagcgta ttacccgtgt ggcgtttgaa tctgcaatga
    10381 aacgtaaaaa acacgtaacc tctgtggata aagccaatgt gttacaaagc tcgattttat
    10441 ggcgtgaaac cgtcacggaa gtagctaaag agttcccaga agtcacgctt gagcatatgt
    10501 atattgataa tgcgacaatg cagctaatca aagcacctga atcgttcgat attttattat
    10561 gctcaaacat cttcggcgat attatttccg atgaggctgc aatgattaca ggttctatgg
    10621 gcatgttgcc ttcagccagc ctcaatgagc aaggtttcgg cttatatgaa ccggcaggcg
    10681 gttcagcacc ggatattgcc ggtaaaaata ttgcgaatcc gattgcgcaa attttatcag
    10741 ccgcaatgat gttgcgttat agctttaact taggtgatgc tgctgatgcg attgaaacag
    10801 cgatccaaaa agcccttgct gatggctacc gtaccgctga tttagctgat gacagcaaac
    10861 cgctttctac ctcagaaatg ggcgatatta ttgcgaaaaa tattctcgct taaggctaga
    10921 agttaaatag cacaagcggt cattattcgc caaatttttg caaattttcc gctaataatg
    10981 accgcttgtt tattactcgt tagccgtcaa tctcttcgcc tgtttcttcc cacttaaaat
    11041 tcaatgccgc agagttgagg caaaaacgca accctgttgg tggtgggccg tccggaaaaa
    11101 cgtgtcccat atgcgaatca caattcccac aacgaatttc gatacgatga cgtcctaaac
    11161 tgtaatcatc taagtaacgt agtgacgatt ctgaaatcgt ttggtaaaaa cttggccagc
    11221 cacaaccggc atcaaattta gtatctgagt gaaataaatc gttatggcat ctggcacaac
    11281 gataaattcc tacccgatgt tcattcaaaa attttccggt aaacggacgt tccgtaccgt
    11341 ggttgattaa aatatcaact tgttgttctg tcagggcgtg aataggttta atcatcagca
    11401 attctcctaa gatagtagcg aagtcattgt agcttttttt gacctgaaaa tcgttataaa
    11461 tttcaaaatt tgacatagat cacaaaaaat ttatctgtta tattggaagg gttcttttgg
    11521 cttgtcagga aagtgaaaag atgtgctata attcaagcat ctttagtgta gtacataaag
    11581 aacaaatttt gtttaacttt aatataggta gaaaaatcta tggcaattaa aattggtatt
    11641 aacggctttg gccgtatcgg tcgtatcgtt ttccgtgcag cacaacaccg tgatgacatc
    11701 gaagtggtag gtatcaacga cttaatcgat gttgattata tggcttatat gctgaaatat
    11761 gattcaactc acggtcgttt tgacggtact gttgaagtta aagacggtca attagtagtt
    11821 aacggtaaag cgatccgcgt aacagctgag cgtgacccgg ctaacttaaa atggaatgag
    11881 atcggtgttg atatcgcagt tgaagcaaca ggtttattct tagatgatga aactgctcgc
    11941 aaacacatca ccgcaggtgc gaagaaagtt gttttaaccg gtccgtctaa agatgcaaca
    12001 cctatgttcg taaacggcgt aaactttgat acttacgcag gtcaagacat cgtgtctaac
    12061 gcttcttgta caactaactg tttagcacca ttagcgaaag ttattcacga aaaattcggt
    12121 atcaaagaag gtttaatgac aactgttcac gcaactactg caacgcaaaa aacagtagat
    12181 ggtccatcag cgaaagactg gcgtggtggt cgtggcgcgt cacaaaacat cattccttca
    12241 tcaacaggtg cagcgaaagc agtaggtaag gtattacctg cattaaacgg taaattaacc
    12301 ggtatggctt tccgtgttcc aaccactaac gtttctgttg ttgatttaac tgtaaactta
    12361 gaaaaaccag caacttatgc agaaatctgt gctgaaatca aacgtgcttc agaaaacgaa
    12421 atgaaaggcg tattaggcta cacagaagat gcagttgttt caacagactt caatggtgca
    12481 actgaaactt cagtatttga tgcagcagca ggtatcgcat taactgatac tttcgttaaa
    12541 ttagtatctt ggtacgataa cgaagttggc tactcaaaca aagtattaga cttagttgc
//
LOCUS       DANLYI010000052        12599 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.052, whole genome shotgun sequence.
ACCESSION   DANLYI010000052 DANLYI010000000
VERSION     DANLYI010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 12599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12599
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.052"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            9..734
                     /gene="fkpA"
                     /locus_tag="PXD81_001100"
     CDS             9..734
                     /gene="fkpA"
                     /locus_tag="PXD81_001100"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HDL5537135.1"
                     /translation="MLKTKLSLFTLIVGTLFSTNVMAEEKADPKFVDDSSYAVGVLMG
                     KNINGVIESQKDIFTYNQDKILAGVQDTLKKTGKLTDEDLQKQLKSLDAYLSAKEAKI
                     NEEKTKATVAAGDKFRADYKKQSGVKETASGLLYKIEKAGEGNSPKAEDTVKVHYKGT
                     LTDGTVFDSSYDRGEPIEFQLNQLIPGWIEAIPMLKKGGKMEIVVPPALAYGDRAAGK
                     IPANSTLKFEIELLDFKAADAKK"
     gene            831..1487
                     /locus_tag="PXD81_001101"
     CDS             831..1487
                     /locus_tag="PXD81_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDL5537136.1"
                     /translation="MTKKFTPFSDEDHAILASYFPMVDGIAALIGEHCEIVLHSLEFL
                     EHSAIYIANGHNTNRKIGSPITDRALKSLHHMATDSVSKPYFTKAKGGVLMKSITIAI
                     RNGKQHVIGLLCININLEVPTSQFLNSFIAPVDTASEVTFASSVEDLVSQTIQTTIDD
                     VMADRQISNNNKNRQIVISLFEKGIFDIKDSINQVAEKLDISRHTVYLYIRQIKQDND
                     "
     gene            1487..1864
                     /gene="tusD"
                     /locus_tag="PXD81_001102"
     CDS             1487..1864
                     /gene="tusD"
                     /locus_tag="PXD81_001102"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012263328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HDL5537137.1"
                     /translation="MDYVLAIKSPVYGSQGAFLAYQVAEELLKANHTICQIFFFQEGV
                     SNANALVNPASDEVNLVEMWKKLAKLHRLPLHLCIAAAQRRGVVDEQISANATHNLAE
                     GFTLAGLGEFSQAVLKADRLLTF"
     gene            1866..2237
                     /gene="tusC"
                     /locus_tag="PXD81_001103"
     CDS             1866..2237
                     /gene="tusC"
                     /locus_tag="PXD81_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HDL5537138.1"
                     /translation="MKKYKLAFLFTQPPFGSAVSREGLDALLAASAFCDEQEIAILFI
                     NDGVFNLIADQQPEKLLQKDHISTFKLLELYDLTECFICQESVSLRGLDNADWLLRML
                     QFKTHAEMIALLNQAEKVMTF"
     gene            2247..2522
                     /gene="tusB"
                     /locus_tag="PXD81_001104"
     CDS             2247..2522
                     /gene="tusB"
                     /locus_tag="PXD81_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HDL5537139.1"
                     /translation="MLYTLSKANYDEETLNFFFSHLSENDAVVLWQDGVLQAVKSPQF
                     FAKLPRCYLLAQDVQARGLQEKLTAFQCISLSELVKLTAVYFPQVAL"
     gene            2638..3537
                     /locus_tag="PXD81_001105"
     CDS             2638..3537
                     /locus_tag="PXD81_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5537140.1"
                     /translation="MVMAEENNTWVDKKHETVSTTLDRWANNINSWLGDADPDKPGTA
                     SLRVMLDSRWDKHDKFTYKPRVRGKIKLPILKKHLNVVFGDDELDNELTDKNHAGKLY
                     KNVNPNKRYNSREARDSNSSIGLRWSDGIKALGIETDLDAGIRSGSDIFGRLRISKDW
                     KITDQFSTRLEQIYRYGSESKHYLRTNFENKYTETDTTFIMNHTNFEYRHDVDEERSW
                     GNSLYRQHNFKPLTHISYGISMGGRLDKELSKFNYWGPFVSYRQPIWRNWLFIQPEIS
                     FYNDKDKDRKHFIQTFVRLEAIF"
     gene            3887..7912
                     /gene="rpoB"
                     /locus_tag="PXD81_001106"
     CDS             3887..7912
                     /gene="rpoB"
                     /locus_tag="PXD81_001106"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HDL5537141.1"
                     /translation="MAYSYSEKKRIRKSFGKRPQVLNVPYLLTIQLDSFEKFIEKDPE
                     GQQGLEAAFRSVFPIVSNNGATELQYVSYELGEPVFDVRECQIRGTTYAAPLRVKLRL
                     VSYDREAAAGTVKDIKEQNVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFARIDRRRKLPATIILRALGYTTE
                     EILNLFFEKIKFEIADNKLLMTLVPERLRGETASFDIAANGKVYVEMGRRITARHIRA
                     LEKDNVTQIEVPVEYIVGKVAAQDYVDLSSGEVICPANMEISMEMLANLSQAGYNQIE
                     VLFTNDLDHGPYISETLRVDPTYDRLSALVEIYRMMRPGEPPTKEAAEALFDNMFFSA
                     ERYDLSAVGRMKFNRSLEIPEGVGSGILSNDDIIGVMKKLIEIRNGRGEVDDIDHLGN
                     RRIRSVGEMAENQFRIGLVRVERAVRERLSLGDLDGVTPQDLINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEVTHKRRISALGPGGLTRERAGFEVRDVHPTHYGRLCPIETPEG
                     PNIGLINSLSVYARTNNYGFLETPFRKVVNGQVTEEIEYLSAIEESNNIIAQANSNLD
                     ENFRFTDTYVTCRQQGESGLFKPEEVQYMDISTQQVVSVAAALIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMEKPIALDSGVAIVAKRGGTVQYVDASRIVVKVNEDE
                     TIPGEAGIDIYNLIKYTRSNQNTCINQVPCVNLGEPVARGEILADGPSTDLGELALGQ
                     NIRVAFMPWNGYNFEDSMLVSERVVQEDRFTTIHIQELSCVARDTKLGAEEITADIPN
                     VGESALSKLDESGIVYIGAEVKGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGTSGTVIDVQVFTRDGVEKDKRAKEIEEMQLCEAKKDLAEELEILEAGLF
                     TRVRNLLIEGGVKESALDGVAREKWLEQTLEDEAKQNQLEQLAEQHEELRKEFERKLE
                     IKRNKIIQGDDLAPGVLKVVKVYLAVKRQIQPGDKMAGRHGNKGVISKINPVEDMPYD
                     ENGQPVEIVLNPLGVPSRMNIGQILETHLGLAAHGIGDQIDKMIKQQQEVAKLREYMQ
                     KAYDLGHGSQVVDLSTFSDEEVMRLAQNLRKGLPLATPVFDGAHESEIKGLLELGGLP
                     TSGQITLYDGRTGEKFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGKA
                     QFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGTHYMEPGIPE
                     SFNVITKEIRALAIDMELDEA"
     gene            8175..12458
                     /gene="rpoC"
                     /locus_tag="PXD81_001107"
     CDS             8175..12458
                     /gene="rpoC"
                     /locus_tag="PXD81_001107"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005618195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HDL5537142.1"
                     /translation="MKDLVKFLKAQTKSNEDFDVIKIGLASPDKIRSWSFGEVKKPET
                     INYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRER
                     MGHIELACPVAHIWFLKSLPSRIGLILDMPLRDIERVLYFESYVVTEPGMTDLEKNQL
                     LTEEQFMDAEERWGDEFEAKMGAEGIQALLRDLDLEHQCETLREELQETNSETKRKKI
                     TKRLKTLEAFLQSGNKPEWMVMTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINR
                     NNRLKRLLDLVAPDIIVRNEKRMLQESVDALLDNGRRGRAITGSNKRPLKSLADMIKG
                     KQGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFRPFIYSKLENRGIA
                     STIKAAKKMVEREDPIVWDILAEVIREHPILLNRAPTLHRLGIQAFEPLLIEGKAIQL
                     HPLVCAAFNADFDGDQMAVHVPLTLEAQLEARALMMSTNNVLSPASGDPIIVPSQDVV
                     LGLYYMTREKVNGKGEGMYFLDPREAEKAYRTGQAELHSRVKVRVTEYEKNEAGELEA
                     KTSLVDTTIGRSILWMIAPKGMPFSLFNQTLGKKAISKLINESYRRLGLKESVVLADQ
                     IMYTGFAYAARSGASVGIDDMVIPEQKYAIIEAAEAEVAEIQEQFNGGLVTAGERYNK
                     VIDIWAAANERVAKAMMENLSTEEVINREGNPEKQASFNSIFMMADSGARGSAAQIRQ
                     LAGMRGLMARPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLT
                     RRLVDVAQDLVIIEDDCGTHEGIVMTPLIEGGDVKEALRDRVLGRVVAEDVLKPGTDE
                     VLIPRNTLLDEKWCDVVDRESVDVIKVRSVVTCDTDFGVCAKCYGRDLARGHLINQGE
                     AVGVIAAQSIGEPGTQLTMRTFHIGGAASAAAKESSIQVKNAGTIKLTNAKFVTNKEG
                     KIVLTSRNTELTVIDTFGRTKENYKVPYGAVLSKHDGAEVAVGEVVANWDPHTMPVIS
                     EVSGRIQFSDIVDGLTVTRQTDELTGLSSIVVQDVGERATAGKDLRPALRLVDSNGKD
                     ILIPGTDVAAQYFLPGKAIVTLDDGAEIEVGEALARIPQESVGTKDITGGLPRVADLF
                     EARKPKEPAILAEISGIVSFGKETKGKRRLVITPAEGEAFEEMIPKWRQLNVFEGEMV
                     QRGDVISDGAETPHDILRLRGVHAVTDYIVNEVQEVYRLQGVKINDKHIEVIVRQMLR
                     KAVITNAYDSEFLEGEQVEVARVKIANRKRAEEGKPLVEFERELLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKQDELRGLKENVIVGRLIPAGTGFAYHQARAKKRAQLEA
                     GIAVVPEFEAPAAKANSFVTDADIEAEFEFVADDATQSLAALLNAGEEE"
BASE COUNT         3810 a         2429 c         2899 g         3461 t
ORIGIN      
        1 gaaaaattat gttaaaaacc aaattatcat tatttactct tattgttggc acacttttta
       61 gtacaaatgt gatggcagaa gaaaaagcag atcctaaatt tgtggatgat tcctcttatg
      121 ccgtgggtgt attaatgggt aaaaatatta atggtgtgat tgagtcacaa aaggatattt
      181 ttacctataa ccaggataaa atcttagccg gtgttcagga caccttaaag aaaaccggta
      241 aattaaccga tgaagatttg caaaagcagt taaaatcgtt agatgcctat ttatctgcga
      301 aagaagcaaa gattaacgaa gaaaaaacca aagcaacggt agctgcaggc gataaattcc
      361 gtgctgatta taaaaaacag tctggcgtaa aagaaacggc ttcaggcctg ttatataaaa
      421 ttgaaaaagc aggtgaaggc aattcaccaa aagcggaaga tactgtgaag gtgcattaca
      481 aaggcacttt gactgatggc acggtatttg acagctctta cgaccgtggt gagccgattg
      541 aatttcagtt aaatcaatta attccgggtt ggattgaagc gattcctatg ttgaaaaaag
      601 gcggtaagat ggaaattgta gtgccacctg cattagctta tggtgatcgt gccgcaggca
      661 aaattccagc aaattcaacc ttgaaatttg aaattgaatt attagatttt aaagcagcag
      721 atgctaagaa ataattcgtt attccttaat tattgaagat acgtaagcgg ttagatttat
      781 tataatattt gcaaaaaatg tgataaaact aaccgcttgt aatttgtatt atgaccaaaa
      841 aatttacgcc tttttctgac gaagatcacg ccattcttgc ttcttacttt ccgatggtag
      901 atgggattgc ggcactgatt ggtgagcatt gcgaaattgt attgcattcg ttagagtttc
      961 tggaacattc ggcaatttat attgctaacg gacacaatac taaccgtaaa atcggctctc
     1021 cgattactga ccgtgcattg aaatcgctgc atcatatggc aacggatagc gtctccaaac
     1081 cttattttac caaggcaaaa ggtggcgttt taatgaagtc tatcactatt gcgattcgta
     1141 atggtaaaca gcacgttatc gggttactgt gcattaatat taatttggaa gtgcctacct
     1201 cacagtttct caattctttt attgcccctg tagataccgc ttcagaagtt acttttgcca
     1261 gttcagtaga agatttggtt tcacaaacga ttcaaacgac cattgatgat gtgatggcag
     1321 atcgtcaaat ttctaacaat aataaaaatc gtcaaattgt gatttcgtta tttgagaaag
     1381 ggatttttga tattaaagat tccattaacc aagtagcgga gaagctggat atttcacgcc
     1441 atactgtgta tctctatatt cgtcaaatta agcaggacaa tgattaatgg attatgtatt
     1501 agcgataaaa tcaccggttt atggttcaca aggggcattt ttagcctacc aagtggcgga
     1561 agaattatta aaagccaatc atacaatttg ccaaatattc ttttttcaag aaggcgtgag
     1621 caatgctaat gccttagtga atccagcaag cgatgaagtg aatttagtcg agatgtggaa
     1681 aaaattggct aaattgcacc gcttgcctct gcatttgtgc attgctgctg cccagcgtag
     1741 aggcgtggtg gatgagcaaa tctcagctaa tgcaacacat aatttagccg aaggatttac
     1801 cctagccggg ttaggcgaat ttagccaagc agtattaaaa gcggatcgat tactgacttt
     1861 ttaagatgaa gaaatataaa cttgcctttt tatttaccca gccacctttt ggttcggcag
     1921 ttagccgtga ggggctagat gccttgcttg cagctagtgc tttttgtgac gagcaagaga
     1981 tagctatcct ctttatcaat gatggcgtat ttaatctcat tgccgatcaa cagccggaaa
     2041 aactcttaca aaaagatcat atttccacct tcaaattatt ggagttgtat gacttaactg
     2101 aatgttttat ttgccaagaa agtgtaagtt tacgagggct ggataatgca gattggttac
     2161 tgcgaatgct tcaatttaaa actcacgctg agatgatcgc attgctcaat caagctgaaa
     2221 aagtaatgac gttttaagga atatttatgc tttataccct ttccaaagcg aattatgatg
     2281 aagaaacctt aaacttcttt ttctcacatc ttagtgagaa cgatgcggtt gttctgtggc
     2341 aagatggcgt gctacaagcg gtcaaaagtc cgcaattttt tgcaaaattg ccccgttgct
     2401 atttattggc acaagatgtg caagctcgag gattgcaaga gaagctcact gcatttcagt
     2461 gcatttcatt atccgaatta gtcaaattaa cagcggttta ttttccccag gttgcactgt
     2521 aacctagaga ggatattttt tgtaatacca aataatttag attatgatgt gattcctaaa
     2581 aaggagaata ttatgaaaac aaaatgtctt attccgctac tagcacttac acctgttatg
     2641 gtgatggcag aagaaaataa cacttgggta gacaagaaac atgaaacagt aagcactacc
     2701 ttagatcgtt gggcaaacaa tattaacagt tggctaggtg atgcagaccc agataaaccg
     2761 ggaacggctt ctttacgggt aatgttagat agccgttggg ataaacacga taaatttact
     2821 tataagcccc gtgttcgtgg taaaatcaaa ctccctatct tgaaaaagca tctaaatgtc
     2881 gtatttggtg atgacgaact ggataatgag cttaccgata aaaatcatgc tggaaaattg
     2941 tataaaaacg tcaatccaaa taaacgctat aactctcgtg aagcgagaga ttcgaactct
     3001 tctatcggtt tacgttggtc tgatggcatt aaggctttag ggattgagac cgatttagat
     3061 gctggtattc gttcgggaag cgatattttc gggcgtttac gcatcagtaa agattggaaa
     3121 atcaccgacc agttcagtac ccgcctagag caaatttatc gttacggttc agagagcaag
     3181 cactatttac gtactaactt tgaaaataaa tataccgaaa cggataccac ctttattatg
     3241 aaccacacca attttgaata tcgtcatgat gtagatgaag aaagaagttg ggggaatagt
     3301 ttatatcgtc aacataactt caaaccactc acgcacatca gctatggtat cagtatgggg
     3361 gggcgtttag ataaagagtt atcaaaattt aactattggg gtccatttgt ctcctatcgt
     3421 caaccaatct ggcgcaactg gctctttatc cagcctgaaa tcagttttta taacgacaaa
     3481 gataaagatc gtaagcactt tattcagaca tttgtacgtt tagaggctat cttctagcta
     3541 ttaagcctca agtagaatct tggggcttgt ttttgcaagc ggtcaatttc ttgccaaaat
     3601 ttacaaaatc cgctataatt cctaaccatt tttagcaaaa attggctgac ggcttgaccg
     3661 gtcagccttt tgtgcttgaa cgcaagggct gattttgtca ttttaatcag caaaatttgc
     3721 aaatttcctg cccaatttga ccgcttgcag tagcgatttc tgactgcttg attggcgatt
     3781 ggaaaatgac atctcgtaca gtttttataa tgccacttcc aaccgagtct ttcgtgtcgg
     3841 attgtgtaat ctcaaccaaa atatatttca gaggctatct taaaaaatgg catattcata
     3901 ttccgagaaa aagcgtatcc gtaagagctt tggcaaacgc ccacaagttc tgaacgtacc
     3961 ttatctatta acaattcagc ttgattcttt tgaaaaattt atcgaaaaag atcctgaagg
     4021 gcagcaaggt ttagaagcgg cattccgttc tgttttcccg attgtgagca acaatggtgc
     4081 aacagaatta caatacgttt cttacgaatt aggcgagccg gtgtttgacg tgcgtgagtg
     4141 ccaaattcgt ggtacaacct acgctgcacc attacgcgtg aaattgcgtt tagtctctta
     4201 cgatcgtgaa gccgcagccg gtacggtaaa agacattaaa gagcaaaacg tgtatatggg
     4261 cgaaatccca ttaatgaccg ataacggtac tttcgtgatt aacggcaccg agcgtgttat
     4321 cgtttctcaa ttacaccgta gcccaggtgt gttcttcgat tctgacaaag gtaaaaccca
     4381 ctcttcgggt aaagtgcttt ataacgcacg cattatccct taccgtggtt cttggttaga
     4441 tttcgagttt gatccgaaag ataacttatt tgcccgtatc gaccgccgtc gtaaattgcc
     4501 ggcaaccatt attctacgtg cattaggcta caccaccgaa gaaatcttaa atttattctt
     4561 cgagaaaatt aaatttgaaa ttgccgataa taaattattg atgacgttag tgcctgagcg
     4621 tttaagaggc gagactgcat catttgatat tgcagcaaac ggcaaagtat atgttgaaat
     4681 gggtcgccgt attaccgctc gtcatatccg tgcgttagaa aaagacaatg tgacccaaat
     4741 tgaagtgccg gtggaatata ttgtcggcaa agtagcggct caagattacg ttgatttatc
     4801 ttcaggtgaa gtgatttgcc cggcgaatat ggaaatctcg atggagatgt tggcgaattt
     4861 atcacaagcc ggttacaacc agatcgaagt attatttacc aacgatttag accacggtcc
     4921 atatatttct gaaaccttac gtgttgatcc aacttacgac cgtttaagtg cgttggttga
     4981 aatctatcgt atgatgcgtc cgggcgaacc accaacgaaa gaagcggcag aagcattatt
     5041 cgataatatg ttcttctcgg cagagcgtta tgatttatcg gcagtaggtc gtatgaaatt
     5101 caatcgctcg ttagaaattc cggaaggcgt aggcagcggt attttaagca acgatgacat
     5161 catcggtgtg atgaaaaaat taatcgaaat ccgtaacggt cgtggtgaag tggatgatat
     5221 cgaccactta ggtaaccgtc gtatccgcag cgttggtgaa atggcagaaa accaattccg
     5281 cattggtttg gtgcgtgtag aacgtgcggt gcgtgagcgt ttatctttag gcgatttaga
     5341 tggtgtaaca ccacaagatt taatcaacgc taaaccgatt tcagcagcag tgaaagagtt
     5401 ctttggttca tcacaacttt cgcaatttat ggaccaaaac aacccgcttt cagaggtaac
     5461 acataaacgc cgtatctcgg cattaggccc gggtggttta acccgtgagc gtgccggctt
     5521 tgaagtgcgt gacgtacacc cgactcacta tggtcgttta tgtccaatcg aaactcctga
     5581 aggcccgaat atcggtttga tcaactcact atcggtgtat gctcgtacta ataactacgg
     5641 tttcttagag actccattcc gtaaagtggt gaatggtcaa gtaaccgaag agatcgagta
     5701 tttatctgcg attgaagaaa gcaacaatat cattgcacag gcgaactcaa acttagatga
     5761 gaacttccgt tttaccgata cttatgtaac ttgccgtcag caaggcgaat cgggcttatt
     5821 caaaccggaa gaagttcaat atatggacat ctcaacccaa caagtggttt ctgtggctgc
     5881 ggcgttaatt ccgttccttg agcacgacga tgcgaaccgt gcgttaatgg gtgcgaacat
     5941 gcaacgtcag gcagttccaa ccttgcgtgc ggacaaacca ttagtcggta ccggtatgga
     6001 aaaaccaatt gctcttgact caggtgtagc gattgtcgca aaacgtggtg gtacggtgca
     6061 gtatgttgat gcgtcacgca ttgtagtgaa agtaaatgaa gatgaaacca ttccgggcga
     6121 agcgggtatc gatatttata acttaatcaa atacacccgt tcaaaccaaa acacctgtat
     6181 caaccaagta ccttgtgtga acttgggtga gccggttgct cgtggtgaaa tcttagcgga
     6241 cggtccttca acagacttag gtgaattggc attaggtcaa aatatccgtg tggcatttat
     6301 gccttggaac ggttataact tcgaagactc aatgttagtt tctgagcgtg tggtacaaga
     6361 agatcgcttt accactatcc acattcaaga attatcttgt gtggcgcgtg ataccaaatt
     6421 aggtgcagaa gagatcaccg ctgatattcc aaacgtgggt gaatctgcat taagcaaatt
     6481 agatgaatca ggtatcgttt acatcggtgc agaagtgaaa ggtggcgaca tcttagtagg
     6541 taaagtaact cctaaaggtg aaacccaatt aacaccggaa gaaaaactat tgcgtgcgat
     6601 cttcggtgaa aaagcctctg atgtgaaaga ttcatcattg cgtgttccaa acggtacttc
     6661 aggtacggtt atcgatgttc aagtatttac ccgtgatggc gtagagaaag ataaacgtgc
     6721 gaaagaaatc gaagagatgc aactgtgtga agcgaagaaa gacttagcgg aagagttgga
     6781 aatcttagaa gccggtttat ttactcgtgt tcgcaaccta ttaattgaag gcggcgtaaa
     6841 agaatctgca ttagatggcg ttgctcgtga aaaatggtta gaacaaaccc ttgaagacga
     6901 agcgaagcaa aatcagttag aacagttagc ggaacagcac gaagagttac gcaaagaatt
     6961 tgaacgtaaa cttgagatca aacgcaacaa aattatccaa ggcgacgatt tagcaccggg
     7021 tgtgttaaaa gtagtgaaag tttaccttgc ggtgaaacgc caaatccagc cgggtgataa
     7081 gatggcgggt cgtcacggta acaaaggggt tatctcgaaa atcaaccctg tggaagatat
     7141 gccatacgat gaaaacggtc agccggttga aatcgtattg aacccgctgg gcgtaccgtc
     7201 tcgtatgaac atcggtcaga tcttagaaac ccacttaggc ttagcggctc atggtatcgg
     7261 tgatcagatt gataagatga tcaaacagca acaagaagtg gctaaattac gtgaatatat
     7321 gcaaaaagcc tacgatttag gtcacggctc acaagtcgtt gatttaagca ctttctctga
     7381 tgaagaagtg atgcgtttag cgcaaaactt acgcaaaggc ttaccgcttg ctactccggt
     7441 attcgatggt gcacacgaaa gcgaaatcaa aggcttatta gagttaggcg gcttaccgac
     7501 ttcaggtcag atcacacttt atgatggtcg taccggtgag aaatttgagc gtccggtaac
     7561 cgttggttat atgtatatgc tcaaattgaa ccacttggtt gatgataaaa tgcacgctcg
     7621 ttcaaccggt tcttatagcc ttgttactca acaaccattg ggtggtaaag ctcaattcgg
     7681 tggtcagcgt ttcggtgaga tggaggtttg ggcattagaa gcatacggtg cggcttatac
     7741 tcttcaagaa atgcttacgg tgaaatcgga cgatgtgaac ggtcgtacga agatgtataa
     7801 aaacattgta gatggtacac attatatgga gccgggtatc cctgaatcct ttaacgtaat
     7861 taccaaagag atccgtgcat tagcaattga tatggagctg gacgaagcgt aatggtttca
     7921 ctctccccct tgatggggga gagacaggca aaggatttgt tcagaatcct ttggcagaga
     7981 gagggtgatg caagcggtgt aaatttgcta aatttttgca aaaatcgacc gcttaccctc
     8041 tccctgattt tgcttctgaa cgaggcaaaa tctatccctc tcccacaagg ggagagggtt
     8101 taaaatcgca accaaattcg tagagtggat attctttcca ccaaacaaaa caacctcaaa
     8161 ctaggggcaa aaaagtgaaa gacttagtga agtttttaaa agcacaaaca aaatcgaacg
     8221 aagattttga tgtgattaaa attggtttag catcaccgga caagatccgt tcttggtctt
     8281 ttggtgaagt gaaaaaaccg gaaaccatta actatcgtac cttcaaaccg gagcgtgatg
     8341 gtttattctg tgcacgtatt ttcgggccgg taaaagatta cgagtgcttg tgtgggaaat
     8401 acaaacgttt aaaacaccgt ggcgtaattt gtgaaaaatg tggtgttgaa gtaacccaaa
     8461 ctaaagtacg tcgtgagcgt atggggcaca ttgaattagc ttgccctgtc gcacacatct
     8521 ggttcttaaa atcactaccg tcccgtatcg gtttaatctt agatatgccg ttgcgtgata
     8581 ttgaacgtgt gctttatttt gaatcttatg tagttactga accgggaatg accgatttag
     8641 agaaaaatca gttattaacc gaagaacaat ttatggacgc tgaagagcgt tggggtgatg
     8701 aatttgaagc gaaaatgggt gcagaaggta tccaagcatt acttcgtgat ttagatttag
     8761 aacaccaatg cgaaaccttg cgtgaagagt tacaagaaac caattcagaa accaagcgta
     8821 aaaaaatcac taagcgttta aaaaccttag aagcattctt acaatccggc aacaaaccgg
     8881 aatggatggt gatgacagta ttaccggtac ttccaccgga tttacgtccg ttagtaccgc
     8941 ttgacggtgg acgttttgcg acttctgact taaacgattt ataccgccgt gtaattaacc
     9001 gtaacaaccg tttaaaacgc ttgttagatt tagttgcacc ggacatcatc gtgcgtaacg
     9061 aaaaacgtat gttacaagag tcggttgatg cgttattaga caacggtcgc cgtggtcgtg
     9121 cgattaccgg ttcgaacaaa cgtccgttaa aatctttggc agatatgatc aaaggtaaac
     9181 aaggtcgttt ccgtcaaaac ttattaggta aacgtgtaga ttactcaggt cgttcggtaa
     9241 ttaccgtagg cccatactta cgcttacacc aatgtggttt accgaagaaa atggcgttgg
     9301 aattattccg tccgtttatc tactcaaaat tagaaaaccg tggcattgct tcaactatta
     9361 aagccgcgaa gaaaatggta gagcgtgaag atcctatcgt atgggatatt cttgccgaag
     9421 tgattcgtga acacccgatt ctacttaacc gtgcaccaac acttcaccgt ttaggtatcc
     9481 aagcgtttga gccgttattg attgaaggta aagcgatcca gttacaccca ctcgtgtgtg
     9541 cggcgttcaa tgcggacttc gacggtgacc aaatggcggt tcacgttcca ttaacccttg
     9601 aagcacagtt agaagctcgt gcgttaatga tgtcaaccaa caacgtactt tcaccggcaa
     9661 gtggtgatcc gattatcgta ccgtctcagg acgttgtact cggtctttac tatatgaccc
     9721 gtgagaaagt aaacggtaaa ggtgagggaa tgtacttcct tgaccctcgt gaagcggaaa
     9781 aagcataccg taccggtcag gctgaattac attctcgtgt taaagtgcgt gttactgaat
     9841 atgagaaaaa cgaagcaggc gaattagaag caaaaacttc gttagtagat accaccattg
     9901 gtcgttcaat cttatggatg attgcaccaa aaggtatgcc gtttagcttg ttcaaccaaa
     9961 ccttaggcaa aaaagcgatc tcgaaattaa ttaacgagag ctatcgccgt ttaggtttaa
    10021 aagaatctgt ggttttagct gaccaaatta tgtacaccgg ctttgcttat gctgctcgtt
    10081 ctggtgcctc agtaggtatc gacgatatgg taattccgga gcaaaaatac gcaatcattg
    10141 aagcggctga agcggaagtc gctgagatcc aagagcaatt caacggcggt ttagtaacag
    10201 ccggtgagcg ttataacaaa gtgatcgata tttgggctgc tgcaaacgaa cgtgttgcga
    10261 aagcaatgat ggaaaacctt tcaacggaag aagtaatcaa ccgtgaaggt aatccggaaa
    10321 aacaagcctc atttaacagt atctttatga tggcggactc cggtgctcgt ggttctgctg
    10381 cacagattcg tcagttagcg ggtatgcgtg gcttgatggc tcgtccggac ggttcgatta
    10441 tcgaaacccc aattactgca aacttccgtg aaggtttgaa cgtattacag tactttattt
    10501 caacccacgg tgcacgtaaa ggtcttgccg atacggcatt aaaaacagcg aactccggtt
    10561 acttaacccg tcgtttagtt gatgtggcac aagatttagt tatcattgaa gatgactgtg
    10621 gtacgcacga aggtatcgtg atgacaccac ttatcgaagg tggtgatgtg aaagaagccc
    10681 tgcgtgatcg tgtattaggt cgtgtggtgg cagaagatgt gttaaaaccg ggtacagatg
    10741 aagtattaat tccacgcaac accttattag atgaaaaatg gtgtgatgtg gtggatcgtg
    10801 aatctgtgga tgtgatcaaa gtacgttcgg tagtaacttg tgatacagac ttcggtgtgt
    10861 gtgctaaatg ttacggacgt gacttggctc gtggtcactt aatcaaccaa ggtgaagcgg
    10921 tcggtgttat tgcggcacaa tctatcggtg agccgggtac acagttaacg atgcgtacgt
    10981 tccatatcgg tggtgcggct tctgcggcag caaaagaatc tagcatccaa gtgaaaaacg
    11041 caggtacgat taaattaacc aacgctaaat ttgtaaccaa caaagaaggc aaaatcgtat
    11101 taacttctcg taatactgaa ttaactgtca tcgacacctt cggtcgtacc aaagaaaact
    11161 ataaagtgcc ttatggtgcg gtgctttcta aacacgacgg tgcagaagtg gctgtgggtg
    11221 aagtggttgc gaactgggat ccgcacacaa tgccggtaat ctcagaggta tcaggtcgta
    11281 tccaatttag cgacatcgta gatggcttaa ccgttactcg ccaaaccgat gaattaaccg
    11341 gtttatcatc tatcgtggtg caagatgtgg gtgaacgtgc aaccgcaggt aaagatttac
    11401 gtccggcatt acgcttagtt gatagcaacg gcaaagacat cttaattccg ggtacagacg
    11461 ttgcagcaca atacttctta ccgggtaaag caatcgtaac cttagacgat ggtgcagaaa
    11521 ttgaagtcgg tgaagcctta gcccgtattc cacaagaatc tgtggggacg aaagatatta
    11581 ccggtggtct tccacgcgta gcagacttat tcgaagcccg taaaccgaaa gagccggcaa
    11641 tcttggcgga aatttcaggt attgtgtcgt tcggtaaaga aaccaaaggt aaacgccgct
    11701 tggtaattac tccggcggaa ggcgaagcgt ttgaagagat gattccaaaa tggcgtcagc
    11761 tcaacgtatt tgaaggcgag atggtacaac gtggtgatgt gatatcagac ggtgcagaaa
    11821 ctccgcacga catcttacgc ttacgtggcg ttcacgccgt taccgactac atcgtaaacg
    11881 aagtgcaaga agtttaccgc ttacaagggg taaaaattaa cgacaagcac atcgaagtga
    11941 ttgttcgcca aatgttgcgt aaagctgtga tcaccaacgc ttacgactct gaattcttag
    12001 agggcgaaca agtggaagtg gctcgtgtga aaattgccaa ccgcaaacgt gctgaagaag
    12061 gcaaaccgtt ggtagaattt gaacgtgaat tgcttggtat taccaaagca tctcttgcaa
    12121 cagaaagctt tatttctgca gcctcgttcc aagaaaccac tcgagtgctt accgaagccg
    12181 cagtggcagg taaacaagat gaattgcgtg gcttaaaaga gaacgtaatc gttggtcgct
    12241 taatcccggc aggtaccggt tttgcttacc accaagcacg agctaaaaaa cgtgcccaac
    12301 tggaagcagg aattgcagtt gtccctgaat ttgaagcacc ggcagctaaa gccaattcat
    12361 tcgttaccga tgcagacatc gaagctgaat ttgaatttgt tgcagacgat gcaacccaaa
    12421 gtttagcagc gttgttaaat gcgggtgagg aagagtaata gctcgtaggg tatgataaat
    12481 taaaaaatct cctagaatat aatttctagg aggttttcta cttgaatata agggaaaaat
    12541 atatagaatg taagggctta ttcaatttgt ggataaggta atcaacagta tgtcaatag
//
LOCUS       DANLYI010000053        12594 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.053, whole genome shotgun sequence.
ACCESSION   DANLYI010000053 DANLYI010000000
VERSION     DANLYI010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 12594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12594
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.053"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            complement(<1..684)
                     /gene="pyrH"
                     /locus_tag="PXD81_001108"
     CDS             complement(<1..684)
                     /gene="pyrH"
                     /locus_tag="PXD81_001108"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005614804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HDL5537143.1"
                     /translation="MSKPIYKRILLKLSGEALQGSEGFGIDPSILDRMALEIKELLAE
                     GVEVGVVLGGGNLFRGAKLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAEVNAKLM
                     SAFQLNGICDTYNWSEAIKMLREKRVVIFSAGTGSPFFTTDSAACLRGIEIEADVVLK
                     ATKVDGVYDKDPAKYADAKLYNKLTYAQVIEQELQVMDLAAFTLARDHGMPIRVFNMG
                     KPGALRNVVF"
     gene            776..2338
                     /locus_tag="PXD81_001109"
     CDS             776..2338
                     /locus_tag="PXD81_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HDL5537144.1"
                     /translation="MKSKQLFGILVAISLATAASCLMLIGSGFFPNPSIFEVILGVFL
                     IIALASSKWTYYLLLLPISIAYAIYTPVGIKFGKPTYEYIASIFATDMMESKEFIAQL
                     PILNIAAGIAIVLAVIFYRKITNRYQIRFLRNKTFVISAFVLMLFSLAPFKFFQEFYN
                     SSLKVKKELEVLNNLTLDSQWGQSTLSSTSNYDDYVLIMGESARKDYHHAYGYPAANT
                     PFMSSAKGTLIDGLTSGGTNTIASLRLMLTKPDTEKWEANYGLTLIDLIKSTGIKTYW
                     ISNQGYLGNYDTPISSIGNKSDVKIFTKAGDSLNTNVSDFELLPHFAKVIEEPAQGKR
                     FIFLHLYGSHPITCDRLTDYPKMFDDDKLPKRYFNVNCYVSSIKKTDELLKRVYDLLV
                     KNQENHQRSFSMVYLSDHGLSHEVSKENIVIHNGKDRSKLHYDIPLFKISSDDTQRNE
                     YKAFKSGLNFTNGIANWIGIENPLLDKEINLFNEQNDKSDYGLKKIIETQSTVEDPAV
                     PIPESYLYKATN"
     gene            complement(2402..3253)
                     /gene="tsf"
                     /locus_tag="PXD81_001110"
     CDS             complement(2402..3253)
                     /gene="tsf"
                     /locus_tag="PXD81_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011609244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HDL5537145.1"
                     /translation="MAEITASLVKELRERTGAGMMECKKALVEANGDIELAIDNMRKS
                     GQAKAAKKAGRVAAEGVILARVASGFGVLVEMNCETDFVAKDAGFLELANEVADFAVA
                     NKGTTIEALQAQFEEKRAALVAKIGENMNIRRVQYLEGEVIAQYLHGAKIGVLVAGAG
                     SEDELKKVAMHVAASKPEFVNPEDVSADVVAKEREIQIEIAMNSGKPKEIAEKMVEGR
                     MAKFTGEVSLTGQPFVMDPSQSVGQYLKSVNTSVSNFIRLEVGEGIEKVEEDFAAEVA
                     KITGGNA"
     gene            complement(3349..4068)
                     /gene="rpsB"
                     /locus_tag="PXD81_001111"
     CDS             complement(3349..4068)
                     /gene="rpsB"
                     /locus_tag="PXD81_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005645552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HDL5537146.1"
                     /translation="MAQVSMRDMLNAGVHYGHQTRYWNPKMKPFIFGARNGVHVINLE
                     KTLPLFNEALAELTRIASNNGKVLFVGTKRAASDAVKAAAVESQQFYVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQTQDGTFDKITKKEALMRTRELEKLELSLGGIKDMNGLPDA
                     IFVIGADHEHIAIKEANNLGIPVFAIVDTNSTPDGVNYIIPGNDDATRAIQLYLDSAV
                     AAIKEGRGVETVEVAEEASAE"
     gene            4413..5441
                     /locus_tag="PXD81_001112"
     CDS             4413..5441
                     /locus_tag="PXD81_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) ABC transporter substrate-binding
                     protein"
                     /protein_id="HDL5537147.1"
                     /translation="MKKTLSALAIAAATFTSTSTLAANEVNVYSYRQPYLIEPMLKNF
                     EKDTGIKVNIIFADKGLVDRVKQEGELSPADVLLTVDISRVMEIVNADLAQKIDSKVL
                     EKNIPAQFRDSNDQWFGLTTRARVIYTSKDRVGKLPAGFDYLDLAKPEYKGKVCVRSG
                     KNSYNVSLFAAMIEHYGIEKTKAFLEGLKANLARKPQGGDRDQVKAIKEGICDYSIGN
                     SYYYGKMLDDEKQKSWAEAAIINFPSGEHGTHKNISGVVIAKHSPNKANAVKLIEYLS
                     GEKAQGLYAELNHEYPVKEGIEPSAIVKGWGTFKSDTIKLEDIAKNYEAALKLVDEVK
                     FDDFSEKK"
     gene            5564..7210
                     /locus_tag="PXD81_001113"
     CDS             5564..7210
                     /locus_tag="PXD81_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HDL5537148.1"
                     /translation="MRSQFCWKSIAALTTFIVLLPLIAICYHALQGDNENLAHLWQTM
                     LPIYLKNSTLLVLGTVFLALIFALPTAWIVANYRFWGQRTLQWLLCLPLAMPAYLIAY
                     LYTDLLDYSGAFQGWLRSIFGWTHPQDYWFPQIRTLYGACFVLALVLYPYIFLLVRVA
                     LLEQSENLTHSAKMLGASPSQLFRRITLPLIRPSVAVGIALVAMETLGDFGTVAYFAV
                     PTLTTAIYDSWLGYHDLGTASQIAIFMLLMIFLFLSLEQYSRRKQKSYQRGYEKKSAF
                     KFLTGWKLALALAWCWGLLALAFFIPFGRLLYWAYDYFEQAWNLDFVQFSLNSLKVSI
                     IASVITVLIALLLHFSKRLNTRKILVKNDRLSLQLASLGYAVPGTVLAIGLLSPLTFA
                     DHQLHAWLKSLELSPVGLIFSGSLFALVLAYTIRFLAMAIGSLETSLNKISPSLDMAS
                     QTLGKNGSAMLVKIHIPLIYKGILTAGLMVFIESMKELNASLLLRPFNFDTLATYVFT
                     FTSDEQLERAALPAIVLVLVGLLPVIWLTRSLISQSEKER"
     gene            7220..8230
                     /locus_tag="PXD81_001114"
     CDS             7220..8230
                     /locus_tag="PXD81_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL5537149.1"
                     /translation="MSVLTINHLNLNFGSTKVLQNLHLAVNQNEIICLLGASGCGKTT
                     LLKAIAGLLPIEQGDIHLAGECLKSKAVEERQIGLIFQDYALFPHLTVADNIQFGLSK
                     LPKVQQKAITEKMLSVVHLQGFESRFPHELSGGQQQRVAIARALACQPELLLLDEPFS
                     NIDSQTRYAMIQEIKQILKSQNVPAIFVTHSKEEAFAFADKIAVMDQGKIIQFGKPNE
                     LYHSPVNPFVADFLGGTNYLNCNVTEDNVLCSPIGEFRLFPEMNVQKGRYRWLLRPEQ
                     IILKPNELGLGKVVGKLFLGLYYRYQVEIKGIEIVTYQSQELKIGEQVEVGFQVREFI
                     LF"
     gene            8240..8866
                     /locus_tag="PXD81_001115"
     CDS             8240..8866
                     /locus_tag="PXD81_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family oxidoreductase"
                     /protein_id="HDL5537150.1"
                     /translation="MKIEVWSDYACPFCYIGKRHLEQALAQFEGEVEVVFRAFELDPH
                     ANGEPEGDIQQRLMRKYQKTAEQADEMIRYVEQAGKQAGLDLRYRTTQYTRTFEAHRL
                     AKFAESKDLGEAMVERLFKAYFTDNTILAKRTELISLALDIGLERDEIAQLLTGDDFG
                     HEVREDERVAHKYGIHSVPFFVINEKLGVSGAQPPEILLDAIKQALQK"
     gene            complement(8928..10418)
                     /locus_tag="PXD81_001116"
     CDS             complement(8928..10418)
                     /locus_tag="PXD81_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 domain-containing protein"
                     /protein_id="HDL5537151.1"
                     /translation="MSLYQIARTETGKVISLNAKMANRHGLIAGATGTGKTVTLRKLA
                     ESLSDDGVPVFLVDVKGDLSGSVQAGNFSGKIAERILQFNLGGEDYLKGYPVSFWDVF
                     GTSGIPLRITISEMGPMLLSRLLNLNDTQGGLLNLVFRVADDRGLLLIDLKDLRALLK
                     FVADNAKEFQLEYGHISAASVGAIQRALLRLENEGADNLFGEPALDLHDWIQTRNGRG
                     VINMLNAEKLINSPRVYSAFLLWFMAELFENLPEVGDPEKPTFVLFFDEAHLLFDDAP
                     KALVDKIEQVVRLIRSKGVGVYFVTQNPLDIPDSVLGQLGNRIQHALRAFTPRDQKAV
                     KSAAETFRANKEVNVVEAITQLGVGQALVSVLDEKGMPTPVEIAYIYPPKSQLAPLAT
                     GERDSLVKQDDLYHHYSQYVDNESAFELLNAKAEAIKAEQAAAEEANNDFFGGILSSI
                     FGSKKKKDQSITEQVVSQVAHSVGRNLRNQVTKQIMRGILGAITKK"
     gene            complement(10477..11712)
                     /gene="rhlB"
                     /locus_tag="PXD81_001117"
     CDS             complement(10477..11712)
                     /gene="rhlB"
                     /locus_tag="PXD81_001117"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HDL5537152.1"
                     /translation="MSNHLTEHRFIDFPIHANVLNALDSKGFEFCTPIQAKTFPITLK
                     GKDIAGQAQTGTGKTLAFLIATFNHLLNNEIETSKNQPRALILAPTRELAVQIGADAE
                     LFLKHSDLKLALAYGGDGYDKQVQEIEKGVDILVGTTGRVIDYVKQGIINLDKIQVIV
                     LDEADRMFDLGFIKDIRYLMRKAPKPSERLTLLFSATLSAKVRELAYEDMDNAEYIEI
                     EPLQKTGHRIKEELFYPSNEDKMALLLTLLEEEWPERCMIFANTKHKCEEIWQYLSAD
                     GHRVGMLTGDIPQKKRLSLLDNFTKGNLDILVVTDVAARGLHIPDVTHVFNYDLPDDK
                     EDYVHRIGRTGRAGESGHSISFACERYAVNLPAIEEYIGHQIPVSQYDKESLLTLPKV
                     NRSRSKKTFLAKTKKNSRN"
     gene            complement(11800..12522)
                     /gene="recO"
                     /locus_tag="PXD81_001118"
     CDS             complement(11800..12522)
                     /gene="recO"
                     /locus_tag="PXD81_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HDL5537153.1"
                     /translation="MTIDQWQRAFVLHRREYSENSLLVEFFTEHHGRITLLAKGARRA
                     RSPLKAVLQPFTPLLLRWSGKGDLKTLTKAEPAALTLPMETLSLYSGFYINEVLARVL
                     ENQTAYPELFQHYLRCITELATQKQIEPILRTFEFHTLKALGYGVDFTHCAATGLPVD
                     PKMTYQFQENQGFIASLLQNNQSFLGSDLLAFAQLDFSEKSTQQAAKRFTRMALKPYL
                     GSQPLKSRELFQSILPNKLKSG"
BASE COUNT         3695 a         2698 c         2345 g         3856 t
ORIGIN      
        1 gaataccaca ttgcgtaacg cccccggttt gcccatatta aagacacgaa ttggcattcc
       61 gtggtcacga gcaagcgtga atgctgctaa gtccattact tgtaattctt gctcaataac
      121 ttgcgcatac gttaatttat tatatagttt tgcatctgcg tattttgctg ggtctttatc
      181 ataaacgcca tcaactttag ttgcttttaa taccacatca gcttcaattt caataccacg
      241 caagcaagca gctgaatcgg tggtaaagaa cgggctacct gtaccggcag agaaaatcac
      301 cacacgtttt tcacgcaaca ttttaattgc ttcagaccag ttgtaagtgt cgcaaatccc
      361 atttaattgg aacgcagaca ttaatttagc atttacctct gcacggtgta aggcatcacg
      421 catagccaaa ccgttcatta cggtggcaag catacccata tggtcgccca ctacacggtt
      481 cattcctgct ttggctaatt ttgcaccacg gaataagtta ccgccgccaa gtaccacacc
      541 aacctcaacg ccttcagcaa gtaattcttt aatctctaac gccatacgat ccaaaataga
      601 cgggtcgata ccaaaacctt cactgccttg tagggcttca ccacttaatt tgagaagaat
      661 tcgcttataa ataggtttgc tcattttaat tgcctcttaa ctgtatggac taaacacaaa
      721 attacgttat tatagaacaa tccaatataa cgataaacta aaaatattca ctacaatgaa
      781 atccaaacaa cttttcggca ttcttgtcgc tatttcgctg gcaactgctg ctagctgttt
      841 aatgctaatc ggatcaggct tttttccaaa tccgagtatc tttgaagtca ttttgggcgt
      901 attcctcatc atcgctcttg ccagctctaa atggacttat tatttactct tactgccaat
      961 cagcatcgct tatgcaattt ataccccggt tgggataaaa ttcggtaaac cgacttacga
     1021 gtatatcgcc tctatttttg ctaccgatat gatggaaagc aaagagttta ttgctcaact
     1081 gccgatttta aatattgcgg cgggcattgc gattgtacta gcggtcattt tttatcgaaa
     1141 aattaccaat cgctatcaaa tccgtttttt acgcaataaa acattcgtga tttcggcttt
     1201 tgtattaatg ctgttttccc ttgcaccgtt taaatttttt caggagttct acaattcgag
     1261 cctaaaggta aagaaagaat tggaagtgct gaacaattta acgctagatt cacaatgggg
     1321 acaatcaacc ctcagctcaa cttcaaacta cgatgattat gtactgatta tgggcgaaag
     1381 tgcgagaaag gattatcacc acgcctatgg ctatccggct gccaatacgc cttttatgag
     1441 ttcggcaaaa ggaaccttga ttgacggttt aacctccggt ggcacaaaca ctatcgcctc
     1501 attacgctta atgctcacta agcccgatac ggaaaaatgg gaagcaaatt atgggttaac
     1561 cttaattgat ttaatcaaat ctaccggtat caagacctat tggatctcaa atcaaggcta
     1621 tttgggcaat tacgatacgc cgatttcctc tatcggtaat aaaagtgatg tcaaaatttt
     1681 cactaaagcc ggcgattcat taaataccaa tgtgagcgat tttgaattgt taccgcattt
     1741 tgctaaagtg attgaagaac ctgctcaagg gaagcgtttc atctttctgc atttatatgg
     1801 ctcgcatcct attacctgcg atcgtctcac cgattacccg aaaatgtttg acgatgacaa
     1861 actgccaaaa cgctacttta atgtaaattg ctacgtgtca tctatcaaga aaaccgatga
     1921 attgctgaaa cgggtatatg atttgctagt gaaaaaccaa gaaaatcacc agcgttcatt
     1981 ctcaatggtt tatttgtctg atcacggttt atcgcacgag gtttcaaaag agaacattgt
     2041 tatccacaat ggaaaggata gaagtaaatt acactacgat attccgctgt ttaaaatttc
     2101 aagcgatgat acccaacgta atgagtataa agcctttaaa tccggcttaa acttcactaa
     2161 tggtattgcg aattggattg gtatcgaaaa tccgttatta gataaagaaa ttaatctttt
     2221 taacgaacaa aacgataaaa gtgattatgg gctaaagaaa atcattgaaa cgcagtctac
     2281 tgtagaagat ccagctgttc ctattcctga aagctactta tataaagcga ctaattagca
     2341 aaagtaagta acaaaaaagc aggctttaag cctgcttttt tagtttttag tatcagaaaa
     2401 attaagcgtt accgccagtg attttcgcta cttctgctgc gaaatcttct tctactttct
     2461 caataccttc acctacttct aaacgaataa agttagaaac tgaagtattt actgatttta
     2521 aatattgacc tacagattga gatggatcca ttacaaacgg ctgaccggtt aatgaaactt
     2581 caccggtgaa tttagccata cggccttcaa ccattttttc tgcgatttct tttggtttac
     2641 cagaattcat cgcaatttca atttgaattt cacgttcttt tgctactaca tcagcagaaa
     2701 catcttctgg gtttacaaat tcaggtttgc ttgcagcaac gtgcattgct actttcttaa
     2761 gctcatcttc agagcctgca cctgctacta atacaccaat tttcgcaccg tgtaagtatt
     2821 gagcaataac ttcgccttct aagtattgaa cacggcgaat gttcatattc tcaccgattt
     2881 tcgcaactaa tgccgcacgt ttttcttcaa attgtgcttg taatgcttcg atagttgtac
     2941 ctttatttgc aactgcaaaa tcagcaactt cgttcgctaa ttctaagaaa ccggcatctt
     3001 ttgcaacgaa gtcagtttca cagttcattt caactaatac gccaaaacct gaagcgacac
     3061 gagcaaggat tacaccttca gctgcaacac gacctgcttt tttagccgct ttcgcttgac
     3121 cggatttacg catattatcg attgctaatt caatatcacc gttcgcttca actaatgctt
     3181 ttttacattc catcatacct gcgccggtac gttcacgaag ttctttaact aatgatgctg
     3241 tgatttcagc cattttaaat tccccttatt tgatatggta aaacaggctc tcacctgcct
     3301 agaaatttca ttgtttaggg caggttaaaa cctgccccct caactgaatt attccgcaga
     3361 agcttcttct gctacttcaa cagtttcaac accgcgacct tctttgattg cagcaactgc
     3421 tgaatctaag taaagttgga tagcacgtgt tgcatcatcg ttaccgggaa tgatgtagtt
     3481 aacgccatct ggagttgagt tagtatcaac aatcgcaaat accggaatac ctaagttgtt
     3541 tgcttcttta attgcgatat gttcgtgatc agcaccgata acaaaaattg catccggtaa
     3601 accgttcata tctttgatac cgcctaagct taactctaat ttttctaact cacgagtacg
     3661 cattaacgct tcttttttag tgattttgtc aaaagtaccg tcttgagttt gagtttctaa
     3721 atcttttaag cgtttgattg attgacgaac tgttttccag ttagtcaaca taccacctaa
     3781 ccaacggtgg tttacgtaga attgttggct ctctactgcc gctgctttaa cagcatcact
     3841 tgctgcacgt tttgtaccaa caaataatac tttaccattg ttactagcaa tgcgagttaa
     3901 ttccgctaat gcttcgttga ataaaggtaa agttttttct aagttgatta cgtgaacacc
     3961 attacgagca ccaaagatga atggtttcat ttttgggttc cagtaacgtg tttggtgacc
     4021 atagtgaacg cctgcgttaa gcatatcgcg catagaaact tgtgccataa gaattttcct
     4081 tcaaagttgt ttgttttgcc acttatgaac ggcaaaacgg ggtttagcct ccacatatcc
     4141 tagaaaatca atctgttaaa gacaccccaa ttcctagctt gtgatatgtg tgtgttttag
     4201 tgttaaaaaa taaatgtgta cgttttgcct gaatagacaa aaacggggcg tattatgcca
     4261 aaaaacaaac aatttgccaa ttattttatt gctctcttcg cattactaaa tcaagctcaa
     4321 aaataaaaac cgttctcatt attgactaaa aaaatgggga ggtttagaat gtctttcatt
     4381 ctgttatgaa aacctgtctg aaggagacta tcatgaaaaa aacactttct gcacttgcta
     4441 ttgctgcagc cacttttaca tcaacttcaa cgcttgctgc aaatgaagtg aatgtttatt
     4501 cttaccgcca gccttactta attgagccaa tgctaaaaaa ctttgaaaaa gatactggca
     4561 ttaaagtaaa catcatcttc gctgataaag ggttggttga tcgtgtaaaa caagaaggtg
     4621 aattaagtcc tgctgatgtg cttttaacag tagatatttc acgcgtaatg gaaattgtta
     4681 acgctgatct tgcacaaaaa attgactcta aagtgttaga aaaaaatatt cctgcacaat
     4741 tccgtgactc taacgaccaa tggtttggtt taaccacacg tgctcgtgtt atctacacgt
     4801 caaaagatcg tgtaggtaaa ttaccagcag gctttgacta cctagattta gctaaaccag
     4861 agtataaagg taaagtctgc gtgcgttcag gcaaaaactc ttacaatgtc tcactattcg
     4921 cggcgatgat cgaacattac ggcattgaaa aaaccaaagc attcttagaa ggcttaaaag
     4981 cgaacttagc acgaaaacca caaggtggtg accgtgatca agtaaaagca attaaagaag
     5041 gcatctgtga ttactctatc ggtaacagct actactacgg caaaatgtta gatgatgaga
     5101 aacaaaaatc gtgggctgaa gcagcgatta ttaatttccc gagtggtgaa cacggcacgc
     5161 acaaaaacat cagcggcgta gttattgcaa aacactcacc aaataaagca aacgcggtga
     5221 aattaattga atacctaagt ggcgaaaaag cacaaggttt atatgctgaa cttaaccacg
     5281 aatatccagt aaaagaaggc atcgagccat cagcaatcgt aaaaggttgg ggtacgttca
     5341 aatcagatac catcaaactt gaagatatcg caaaaaatta cgaagccgca ttaaaattag
     5401 ttgatgaagt gaaatttgac gatttctctg agaaaaaata atcaaccgat aagcggtcat
     5461 ttttccacaa aaatttgcaa aattttgtta aaatacaacc gcttgcaaaa ggacgcattt
     5521 tgcgtccttt tattttttta tgaaataaca ggcttaaaga aaaatgcgat cacaattttg
     5581 ctggaaaagt attgctgccc taacaacttt tatcgttttg cttcctctta ttgcaatttg
     5641 ctatcacgca ttgcaagggg ataatgaaaa ccttgctcac ctttggcaaa caatgttgcc
     5701 aatctatctt aaaaactcaa ctttattagt attaggaacc gttttccttg cactgatttt
     5761 tgctctgcct accgcttgga ttgtggcaaa ttaccgtttt tgggggcaaa gaaccttaca
     5821 atggttactt tgtttgcctc tcgctatgcc tgcttattta attgcctatt tatataccga
     5881 tttattagat tactcaggtg cttttcaagg ctggctgcgt agtatttttg gctggactca
     5941 tccgcaagat tactggtttc ctcaaattcg tacattgtat ggtgcttgct ttgtgcttgc
     6001 gttagtacta tatccataca tttttttatt agtgcgagtt gccttattag aacaatctga
     6061 aaacttaaca cacagtgcaa aaatgctcgg ggcttcgccc tctcagttgt tccgtcgaat
     6121 tacgctgcca ttaattcgac cttctgttgc cgtaggcatt gcattagtgg caatggaaac
     6181 gcttggcgat ttcggtactg tggcctattt tgccgtgcca acacttacta cggcaattta
     6241 tgattcgtgg ctaggctatc acgatcttgg tactgccagc cagattgcga tctttatgtt
     6301 gctaatgatt ttcctgtttt taagtttaga gcaatatagt cgccgtaaac aaaaaagcta
     6361 tcaacgtggc tatgaaaaga aatcagcctt taaattttta actggttgga aattagcatt
     6421 agcccttgct tggtgctggg gtttattggc gttagccttt ttcattccgt ttggcaggtt
     6481 gctctattgg gcatacgact atttcgagca agcgtggaat ttagactttg tccaattttc
     6541 gcttaatagt ttgaaagtat cgatcatcgc ctccgtgata acggtactga ttgctctact
     6601 tctgcatttt tctaaacgtt tgaatacccg caaaatttta gtcaaaaatg accgcttgtc
     6661 attacaactt gcctcactgg gctatgcggt tcctggcaca gtattagcga tcggtttact
     6721 ttcgccttta acatttgccg atcatcaact ccatgcttgg ctaaaaagcc ttgaattatc
     6781 acccgttgga ttgatttttt caggctcact ctttgcatta gtattagctt ataccatccg
     6841 atttttagca atggcaattg gcagccttga aacttcgcta aataaaatct ctccatcgct
     6901 ggatatggcg agccaaacgc ttggtaaaaa tggctcagca atgttagtaa aaattcatat
     6961 ccctttaatt tataaaggta ttttaactgc aggattaatg gtctttattg agagtatgaa
     7021 agagctaaat gcttctctgc tacttcgccc atttaatttt gatacactcg caacctatgt
     7081 gtttaccttt acatcagacg agcagcttga gcgtgctgct ctgcctgcca tcgtattggt
     7141 gttagtcggt ttactgcctg ttatttggct taccagatcc cttatttcgc aatctgaaaa
     7201 agaaagataa aggaatttta tgtctgtttt aactattaat catctcaacc tcaatttcgg
     7261 cagtacaaag gttttacaaa atctccatct tgctgtgaat caaaatgaaa ttatctgttt
     7321 gctaggtgca agtggctgtg ggaaaactac actgctcaaa gccattgcag gtttattgcc
     7381 aattgagcag ggagatattc atttagcggg tgaatgccta aaaagtaaag ccgtggaaga
     7441 gcgacaaatc gggctgattt tccaagatta cgctcttttt ccacacttga cggtagccga
     7501 taatattcaa tttggcttat ccaaattgcc aaaagtacaa caaaaagcaa ttaccgaaaa
     7561 aatgctttca gttgtgcatt tacaaggctt tgaaagccgt tttccacacg aactttcggg
     7621 cgggcaacaa cagcgtgtcg ctattgcccg agcattagcc tgccagccag aattgctact
     7681 gttagatgag ccgttttcca atattgacag ccaaactcgc tatgcaatga ttcaagagat
     7741 caagcaaatc ttaaaaagcc aaaatgtgcc cgctattttt gtaacccata gcaaagaaga
     7801 agcctttgca tttgccgata aaattgccgt gatggatcaa gggaaaatta ttcaatttgg
     7861 taagccaaac gagctatacc attctccagt taatccattt gtggcagatt ttttaggtgg
     7921 cacaaactat cttaactgta atgtaacgga agacaacgtg ttatgcagcc ctattggcga
     7981 gttccgactc ttccctgaaa tgaacgttca gaaagggcgt taccgttggc ttcttcgccc
     8041 agaacagatt atcttaaaac ccaatgagct aggtttaggt aaagtggtag gcaaactgtt
     8101 tttaggccta tactaccgct atcaggttga aattaaaggc attgagatag taacttacca
     8161 atctcaagag ctgaaaattg gcgaacaagt ggaagtgggc ttccaagtaa gagagtttat
     8221 cctattttaa ggagaatata tgaaaattga agtttggtca gattatgcct gcccgttttg
     8281 ttacatcggc aaacgccact tagagcaagc cctagcccaa tttgaaggcg aggttgaagt
     8341 ggttttccga gcctttgagc ttgatccgca cgctaacggc gaaccggagg gcgatattca
     8401 gcaacgccta atgcggaaat atcaaaaaac cgcagaacaa gctgatgaaa tgattcgtta
     8461 tgtggaacaa gccggcaaac aagctgggct agatttgcgt taccgcacca cccaatacac
     8521 tcgcaccttt gaagctcatc gcttggcgaa atttgcagaa agtaaagact taggcgaagc
     8581 aatggttgaa cgtttattca aagcctattt taccgataac actattctag ctaaacgcac
     8641 agagcttatc agtttagcct tagacattgg cttagagcgt gatgaaatcg ctcaactgct
     8701 cacaggtgat gatttcgggc acgaagtccg tgaagatgag cgagtcgctc acaaatatgg
     8761 cattcacagc gtaccgtttt ttgtgatcaa tgaaaaactc ggggtatccg gggctcaacc
     8821 gccagaaata ttacttgacg ctatcaaaca ggcattgcaa aaataatccg aaaaaagacc
     8881 gcttgttaag ctaaacaagc agtcttattc ttcttataat ttacaattta ttttttggtg
     8941 atcgcaccta aaataccacg cataatttgt ttggtgactt ggttgcgtaa atttctgcca
     9001 actgaatgcg ccacttggct aaccacttgt tcggtaatcg attgatcttt tttcttcttc
     9061 gagccaaaaa tggaagagag aataccgcca aagaagtcat tgtttgcttc ttctgccgca
     9121 gcctgctctg ctttaattgc ttctgctttc gcattaagta attcaaacgc agattcatta
     9181 tcgacatatt ggctgtagtg atgatagaga tcatcttgct tcactaaact atcacgttcg
     9241 cctgtggcta atggagctaa ttggcttttt ggtggataga tataggcaat ttctacaggc
     9301 gttggcatac ctttttcatc tagcaccgaa accaacgctt gccccacacc aagttgcgtg
     9361 atggcttcta ccacatttac ctctttattc gctcggaaag tttccgctgc tgattttacc
     9421 gctttttgat cacgtggcgt gaaagcacgc aaagcgtgtt ggatacgatt gccaagctgc
     9481 cctaatactg aatcaggtat gtccaacgga ttttgcgtaa caaaataaac acccacccct
     9541 tttgagcgaa ttaaacgaac cacctgttca attttatcca ccaatgcctt aggggcatcg
     9601 tcaaacaata aatgagcttc atcaaagaat aaaacaaacg tcggtttttc tgggtcgcca
     9661 acttctggca aattctcaaa gagctctgcc ataaaccaaa gcaggaatgc actgtacact
     9721 cttggtgagt taattaattt ttctgcattc aacatattga ttacaccacg cccattacgg
     9781 gtttggatcc aatcgtgcaa atctaatgca ggctcaccaa ataaattatc tgccccttca
     9841 ttttcaagcc ttaataacgc acgctgaata gctccaacgc ttgctgcaga aatatgccca
     9901 tattccagtt ggaattcttt cgcattatct gcgacaaatt taagcaacgc acgcaaatct
     9961 tttaaatcaa ttagcaataa acctctgtca tctgccacac gaaaaacgag atttaacaag
    10021 cctccttggg tatcgtttaa attgagtaaa cgagaaagta acatcggtcc catttcggaa
    10081 atagtgattc gaagtgggat acctgatgta ccaaatacgt cccaaaacga aactggatag
    10141 ccctttaagt aatcttctcc ccctaaatta aactgtaaaa tgcgttctgc aatttttcct
    10201 gaaaaattac ccgcttgtac cgagcccgaa agatcacctt ttacatctac taaaaatact
    10261 ggcacgccat catcactcaa agattctgcc aatttgcgta aagtaaccgt tttacctgta
    10321 cccgtagccc cagcaattaa accgtgccga tttgccattt tggcattaag cgaaataact
    10381 ttgcctgttt ctgttcgagc aatttgatac aaactcatct ttcattctct taaaggtaaa
    10441 ttaatttttt gttctcatca tataaaaatt tatcacttaa ttacggctat tctttttcgt
    10501 ttttgccaag aacgtttttt tacttcttga gcggttcact ttcggtaaag tcagtaagga
    10561 ttccttgtcg tattgactca caggaatctg atgcccaata tactcttcaa ttgcaggcaa
    10621 attaaccgca taacgctcgc aagcaaaact aattgaatgg ccgctctccc ctgctcgccc
    10681 cgttctacca atacgatgaa cgtaatcttc tttatcatca ggcagatcat aattgaaaac
    10741 gtgcgtgaca tctggaatat gcaatccacg agccgcaaca tctgttacca ctaaaatatc
    10801 caaattgcct ttggtgaaat tatccagcaa agaaaggcgt tttttctgcg gaatatcgcc
    10861 ggttagcatt ccaacccgat gtccgtctgc cgataaatac tgccagatct cttcgcattt
    10921 atgcttggtg ttggcaaaaa tcatacaacg ctccggccac tcttcctcaa gcaatgtcag
    10981 caacaatgcc attttgtctt cattcgaggg gtaaaatagc tcttctttaa tacgatgtcc
    11041 ggttttttgt agtggctcaa tttcaatata ttccgcatta tccatatctt cataggcaag
    11101 ttcccgcact tttgccgaaa gcgttgccga aaagagcaaa gttaaacgtt ctgaaggctt
    11161 aggtgctttt cgcattaaat aacggatatc tttaataaaa ccaagatcaa acattcgatc
    11221 tgcttcatct agcacaatca cttgaatttt gtctaaattg ataatgcctt gttttacata
    11281 gtcaatcact cgacctgttg taccgacaag aatatctacc cctttttcaa tttcttgaac
    11341 ttgtttgtcg taaccatcac caccgtatgc caaagctagt tttaaatcag aatgttttaa
    11401 aaacagctcg gcatctgccc caatttgaac cgccaattca cgggttggag caagaattaa
    11461 tgctctcggc tgattttttg acgtttcgat ttcgttattc aataaatggt taaaagttgc
    11521 aatcaaaaag gcaagcgttt tccctgtacc tgtttgagct tgccctgcaa tatctttgcc
    11581 tttcagcgta atcggaaagg tttttgcctg aatcggcgta caaaattcaa agcctttgct
    11641 atcaagggca tttagtacat tagcgtgaat cgggaagtca ataaagcggt gttcggtaag
    11701 atgattagac atagtatttc cttgataatt gtgatgtgac aagcggtctt ttttacacat
    11761 ttttttgcaa aatagcatat tgggcgtttt ttctcaaatt taaccgcttt tgagtttatt
    11821 cggcaaaata gattggaaaa gctctcggct tttcagcggt tgtgacccta aatagggctt
    11881 tagtgccatt cgggtaaaac gtttcgctgc ttgttgagta cttttttctg aaaaatccaa
    11941 ctgagcaaac gctaataaat cactgcctaa aaagctctgg ttattctgta ataatgaggc
    12001 gataaagccc tgattttcct gaaattgata ggtcattttc ggatcaactg gcaaaccggt
    12061 tgccgcacag tgcgtaaaat ccaccccata acccaatgct tttaacgtat gaaattcaaa
    12121 ggtgcgtaaa atcggctcaa tctgtttttg tgtggcaagc tcggtaatgc aacgtaaata
    12181 atgctgaaat aattcagggt aagcggtctg attttccaat actcttgcaa gtacctcgtt
    12241 gatataaaac ccactataaa gtgaaagcgt ttccatcggc aacgttaatg cagccggttc
    12301 tgctttggtt aaagttttca gatccccttt acccgaccaa cgcaacaaca acggcgtgaa
    12361 cggctgtaaa accgctttca acggcgagcg agcccgcctt gcacctttag caagtaaagt
    12421 aatccgcccg tgatgttcgg taaaaaactc cactaataaa ctgttttcgc tgtattcacg
    12481 ccgatgaagg acaaaggctc gttgccattg gtctattgtc ataatttctt gttaattatg
    12541 aggttgtaat ttacttctgc caaatctccc cctcccctct ttgctaaaga gggg
//
LOCUS       DANLYI010000054        12584 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.054, whole genome shotgun sequence.
ACCESSION   DANLYI010000054 DANLYI010000000
VERSION     DANLYI010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 12584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12584
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.054"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            complement(<1..654)
                     /locus_tag="PXD81_001119"
     CDS             complement(<1..654)
                     /locus_tag="PXD81_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5537154.1"
                     /translation="MADHSQFISRLENRIEPKISFQELIQELSNYTNKPFSHIADKLK
                     SLIFSFQQNGIYTNAFVNCTLFEDGESYAIEPKTLHFKDAFDQPKGVLSDVIAQNSID
                     VEHLNAYYVTAREITQLIKTQSPTLLDKLTTKQTQSEISQIQNDYISVYDFIEWASKH
                     YSSLSETANDLNRLLKDKNIQLYRQYGGISPSIDKDNTNLKAAFDFVAINNGYEKQSS
                     "
     gene            complement(1049..1372)
                     /locus_tag="PXD81_001120"
     CDS             complement(1049..1372)
                     /locus_tag="PXD81_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative addiction module antidote protein"
                     /protein_id="HDL5537155.1"
                     /translation="METIQINEQAAEKHGLRPFDVAEFLTDEATIQAYLAEVLKEGDQ
                     DEFLEALNDVARARGMAELAKQTGLGRESLYKTLAKGSKPRFDTIQKILGAFNLVLIP
                     QVKNG"
     gene            complement(1485..1703)
                     /locus_tag="PXD81_001121"
     CDS             complement(1485..1703)
                     /locus_tag="PXD81_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module killer protein"
                     /protein_id="HDL5537156.1"
                     /translation="MMREQTTNQIIITDNFSKWLENLKNPIAKATVARRIKNAINGNF
                     GDHKSLAGTGGLYEMRIATGAGYRIYYA"
     gene            complement(1978..3204)
                     /locus_tag="PXD81_001122"
     CDS             complement(1978..3204)
                     /locus_tag="PXD81_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDL5537157.1"
                     /translation="MARKITPLNDTQIRKAKPEDSPLRDGNGLLLVITSNSKLWRFRY
                     ERPFTKKRNDLSIGAYPDISLAQAREIREEYRALLAQNIDPQTHRQQQEQAKANEISN
                     TYENVAWAWLEHRKTKKNFSDNYQKDVIALIKNNLLPHFGRMPITQITAPMAIKAFKQ
                     YQDEGKLEKLKRAIQKHNEIMMFAVHREMIPFNPTANIAKEFDSPTVEHFKTIKPEDL
                     GEFLYTLQNAQIHLQTRYLILWQLLTMTRPNEAAKAKWADIDEKAKLWTIPAENMKRG
                     IEHKITLSRQAIALLREIKKLSGGKTYLFPSVKNPQTHVNTQTANGAIKRMGYKGKLV
                     AHGLRAIASTYLNEKGYNSDLIEVALSHLNSDRIKAAYDRGERLEQRFKLLQVWADFV
                     EECSQGSLPQYHLKIA"
     gene            complement(3479..3845)
                     /gene="ssrA"
                     /locus_tag="PXD81_001123"
     tmRNA           complement(3479..3845)
                     /gene="ssrA"
                     /locus_tag="PXD81_001123"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            4011..4970
                     /gene="rpoH"
                     /locus_tag="PXD81_001124"
     CDS             4011..4970
                     /gene="rpoH"
                     /locus_tag="PXD81_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HDL5537158.1"
                     /translation="MKKFNENEIEDAEIIDAEPIDVDEIDEEETSVEAMHPALVSGSM
                     PVPQGNLDSYIRMANQYPILTAEQEKELAERYYYDEDVEAAKQLILSHMRFVIHIARG
                     YLGYGLPLADLIQEGNIGLMKAVKRFDPNVGVRLVSFAVHWVKAEIHEYVLKNWRIVK
                     VATTKAQRKLFFNLRKNKNRLAWFNEEEIKKVADDLGVSVEEVREMESRMTGQDLGFD
                     LPAGDDDEDSYAPSMYIEDSSSNFADDLEDEQHNGQATAQLAYALATLDERSQDIIKT
                     RWLDDNKATLQDLADKYNISAERVRQLENAALKKIKEAISLDG"
     gene            5136..5429
                     /locus_tag="PXD81_001125"
     CDS             5136..5429
                     /locus_tag="PXD81_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3144 domain-containing protein"
                     /protein_id="HDL5537159.1"
                     /translation="MTQLSTSVYQRADGFINVANAHLKNIAPNQVSNAMLFGCARFNA
                     YVAASKAEYKQQLADSREEVIQYFVEQYKEMLTANLDEYIQNFERYIEGKKAD"
     gene            complement(5447..5749)
                     /locus_tag="PXD81_001126"
     CDS             complement(5447..5749)
                     /locus_tag="PXD81_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="HDL5537160.1"
                     /translation="MNTYNNTLNNKNLEKKQLLMAIQAIPASQDFIWDGKDDDERPVT
                     PSEFSIAMKKRGRPAGSKKELVSLRLDKSVLEAFRASGKGWQSRINQVLSDFVKQV"
     gene            complement(5721..6011)
                     /locus_tag="PXD81_001127"
     CDS             complement(5721..6011)
                     /locus_tag="PXD81_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HDL5537161.1"
                     /translation="MKIYTWDETKRKINLAKHGLDFADADEVLSSPYRFDVEVIRNNE
                     FRIQSFAYSFGRLLVLSLAYTEREDYIRVISFRRASKAEREAYYEYLQQYFE"
     gene            complement(6149..6688)
                     /locus_tag="PXD81_001128"
     CDS             complement(6149..6688)
                     /locus_tag="PXD81_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIA family hydrolase"
                     /protein_id="HDL5537162.1"
                     /translation="MLDLTKIKLVITDVDGVLTDGGMYYTDQGEVMKRFHVHDGLGVK
                     MLQSCGIKVAVLSGGDTALLRKRLEVLKIDLALLGKMEKRSACFELMQQAGVTPEQTA
                     YIGDDTLDLPAFEVCGIAIATRNALDYIKEKADWVLQKAGGEGAFREVSDQILEAQGF
                     GEIFKTADGFLTVAGKMAQ"
     gene            6759..7436
                     /locus_tag="PXD81_001129"
     CDS             6759..7436
                     /locus_tag="PXD81_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HDL5537163.1"
                     /translation="MSIAENLSQIHQQIQQISEQYQRDNVRLLAVSKTKPVEAIQAAI
                     EAGQRAFGENYVQEAVEKIEFFANRPELEWHFIGPLQSNKTKLVAAYFDWIQTVDRLK
                     IAERLNEQRPADKAPLNVLIQINISDEASKSGIEPDEMLPLAQAISQLPNLKLRGLMA
                     IPKPESEPEQQKVAFRKMQLLFNRLQTEFDDIDTLSMGMSDDMQAAIECGSTMVRIGT
                     AIFGARR"
     gene            7621..9468
                     /gene="typA"
                     /locus_tag="PXD81_001130"
     CDS             7621..9468
                     /gene="typA"
                     /locus_tag="PXD81_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HDL5537164.1"
                     /translation="MQNVDISKLRNIAIIAHVDHGKTTLVDKLLQQSGTFGETRGDVD
                     ERVMDSNDLEKERGITILAKNTAINWNGYHINIVDTPGHADFGGEVERVLSMVDSVLL
                     VVDAFDGPMPQTRFVTQKAFAHGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLGASD
                     EQLDFPIIYASALNGVAGLEHEDLAADMTPLYEAIVKHVQPPQVELDAPFQMQISQLD
                     YNNYVGVIGIGRIKRGSVKPNQAVTIVDSEGKTRQGRIGQVLGHLGLQRFEATEAFAG
                     DIVAITGLGELNISDTICDINAVEALPALSVDEPTVTMFFCVNTSPFCGQEGKFVTSR
                     QILERLNKELVHNVALRVEETSNPDEFRVSGRGELHLSVLIENMRREGYELAVSRPKV
                     IYREEDGKKQEPFEQVTIDIEEQHQGSVMEALGIRKGEVRDMIPDGKGRTRLEYVIPS
                     RGLIGFRNEFMTMTSGTGLLYSTFSHYDDVKPGEIGQRKNGVLISNATGKALAYALFG
                     LQERGKLMIDHGVDVYEGQIIGIHSRSNDLTVNCLQGKKLTNMRASGKDDAIVLTTPV
                     RFTLEQALEFIDDDELVEVTPQSVRIRKRLLTENDRKRASRTTTSTSTH"
     gene            complement(9517..9954)
                     /gene="rimI"
                     /locus_tag="PXD81_001131"
     CDS             complement(9517..9954)
                     /gene="rimI"
                     /locus_tag="PXD81_001131"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005616368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HDL5537165.1"
                     /translation="MIEQIQAEDFEQLFEIEQQAHLVPWSKGTLLNNQGEKYLNLKVV
                     EQNKIVAFAISHIVLDEATLFNIAVAPDFQGKGIGKRLLSELILQLQQRKLTTLWLEV
                     RESNHSARKLYDSLGFNEVTTRKNYYPTPNGSKENAVVMALYL"
     gene            complement(9947..10369)
                     /locus_tag="PXD81_001132"
     CDS             complement(9947..10369)
                     /locus_tag="PXD81_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HDL5537166.1"
                     /translation="MNRRDLLLQEMGISQWVLTNPQVLKGDAQIRLNNAVKLVVVCEE
                     DHQHSRLFADILRALKLKNSEYQWLTGEQSQRLVFEHSPIFWLIQAEEQAVKMAKSFT
                     NQTAWKNESWQDLAKSTTKRQLWQQIDTFSAQFGEQRD"
     gene            10499..11488
                     /gene="rsmC"
                     /locus_tag="PXD81_001133"
     CDS             10499..11488
                     /gene="rsmC"
                     /locus_tag="PXD81_001133"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HDL5537167.1"
                     /translation="MLSLESEVLERHLALFENKSVLLFGDVRDDFARQLQSRAEKVAV
                     FSSYFDYARHHSEVEFGLECGLSAELAVFYWTKNKQECQFQLLQWLSQCQAGQEMLII
                     GENRAGVRSVEKLLEPFGNIAKIDSARRCGLYHFELQTVPTFDCKKFWKSYRLQDLDV
                     FDLPAVFSSAELDNGTKLLLSTFDKADRLKGKVLDLGCGAGVIGATLKQQFPKIKLTM
                     SDIHAMALESGRRTLAENQLEGEVVASDVFSHINERFDLIVSNPPFHDGVDTAYRAVE
                     ELIFQAKNHLTKGGELRIVANAFLPYPDLLDKAFGKHDVLAKSTKFKVYSART"
     misc_feature    11618..11740
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            11762..12139
                     /locus_tag="PXD81_001134"
     CDS             11762..12139
                     /locus_tag="PXD81_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor"
                     /protein_id="HDL5537168.1"
                     /translation="MKNPIEIYQSQDGKTQVSVQFDKETVWLSLQQMADIFGRDKSVI
                     SRHLRNIYQEGELEREATVAKNATVQIEGAREVVRLKDYLVKGYALNERRLQERGIEF
                     EQVIGLLSQYTGCLSLVGGRKFA"
     gene            12206..>12584
                     /locus_tag="PXD81_001135"
     CDS             12206..>12584
                     /locus_tag="PXD81_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL5537169.1"
                     /translation="MSINISPLASKNLTEWKLLWQQYLDFYQVNLADDIIQSTWQKVS
                     ESENVKGFGAFVDNRMVGFVHIVIHPNTWNLTECCYLEDLFVLAEYRNQGIGKELIEY
                     IYRYAQEKGYNRVYWVTDKNNHQA"
BASE COUNT         3622 a         2493 c         2682 g         3787 t
ORIGIN      
        1 gcttgattgt ttttcatatc cgttattaat tgctacaaaa tcaaaggctg cttttaaatt
       61 agtattatct ttatcaatgc ttggacttat tccgccatat tgtcgataaa gctgtatatt
      121 tttatctttg agtaagcggt ttaaatcgtt agctgtttca ctaagacttg agtaatgttt
      181 acttgcccat tctatgaagt cataaacaga aatataatca ttttgaattt ggctaatctc
      241 tgattgtgtt tgcttggtgg ttaatttatc taacaaggtt ggcgattgtg ttttaatcag
      301 ctgtgttatt tctcttgctg ttacgtaata agcatttaaa tgttcaacat caatagaatt
      361 ttgagcaata acatcactta aaacgccttt aggctgatcg aaagcgtctt tgaaatgtag
      421 tgttttgggc tcaatagcat agctttcacc atcttcaaag agagtgcaat ttacaaaagc
      481 attcgtatat attccatttt gctgaaagga aaaaatgagg cttttcaatt tatccgctat
      541 atgtgaaaaa ggtttatttg tatagtttga taattcttgt atcagttctt gaaagctgat
      601 tttgggctca atacgatttt caagcctaga aataaattga ctgtgatctg ccataagcgc
      661 ccctttaacg tatcccttta ctgtgagagc gtggaaagag aaataaaggg ctttctcctt
      721 tcgtgccggt taattactcc gccactatcc acgctaattt gaataaatcc gtatagttac
      781 ggattgaact tgataggttg tattttgcta attattcaga taaattacaa gtaataagct
      841 gtgttttatt acagtaaatc aaaataggct aaagatgggt ttccggacgc tataaaaaag
      901 agcattcaag cggtcatatt tcattaaggt tttgcgaata gtgcggaatt ttcggagcag
      961 tacgcacgca aggagcaaat aaaaagccgt caatatccat caacggctat ttaaaagtga
     1021 ggttttgaca ggtagcaagt gataagtttt agccattttt gacttgtgga ataagcacta
     1081 aattgaatgc ccctagaatt ttttgaatag tgtcaaagcg tggtttgctg cctttggcta
     1141 gggttttata taggctctct ctgcctagcc ctgtttgctt ggccagctcc gccattcctc
     1201 ttgctcttgc tacatcgttc aaggcttcta aaaattcgtc ttggtcgccc tcttttaaca
     1261 cttccgctaa ataggcttga atagttgcct catcggttaa aaactccgcc acgtcaaaag
     1321 ggcgtaaacc gtgcttttct gctgcctgtt cattgatttg tattgtttcc attgctttct
     1381 cctgtagttt ttaattcagc ccataatgct ttcgctttct ctatatcggc ttgctgtgtt
     1441 gatttatcgc cgccgtgtaa caagatataa attacatttc cttgttatgc gtaataaatt
     1501 ctatatccgg ctcctgttgc tatccgcatt tcataaagtc cgcctgtacc ggctaaagat
     1561 ttatgatcgc caaagttgcc attaatcgca ttttttatcc gtcttgctac cgttgctttt
     1621 gcgatagggt tctttaaatt ctcaagccat tttgaaaaat tatcggtaat aataatttga
     1681 ttagtggttt gctctctcat cattgccggc ttcttattct atttattgct tattgtatcc
     1741 attaagatac agtgagcaag ggtttttgcc atttacccac ttttaaccca ctttacccac
     1801 tgtcattttt tctttatttt tcagttagtt ataaaaaaat aatagtgaaa agtgggtaaa
     1861 gtgggtaaaa atcgaaaagt ttacagaatt ttcatcaagg ggaattacgg gcataaaaaa
     1921 gccgtcaggc ggtttaccta acggcattac aagcggtcat ttttggcgga aaatttacta
     1981 ggctattttc aaatgatatt gcggtaagct accttgcgaa cattcctcta caaaatccgc
     2041 ccacacttgg agcagtttaa aacgttgctc taggcgttcg cctctatcat aggcggcttt
     2101 gattcggtcg ctgtttaaat gcgataaggc aacctcgatt aaatcgctat tgtagccttt
     2161 ctcattaaga taagtggagg cgatggcccg caagccgtga gccactaatt tgcccttgta
     2221 gcccattcgt ttgatcgcac cgttggcggt ttgagtattg acgtgcgttt gtgggttctt
     2281 cacgctaggg aataaatacg ttttaccgcc acttagcttt ttaatctctc gcaaaagtgc
     2341 gattgcttgg cgtgagagtg tgattttatg ctcaatgcct cgcttcatat tttccgccgg
     2401 tatcgtccac agtttggctt tttcgtctat atccgcccat tttgccttag ccgcttcatt
     2461 cgggcgggtc attgtgagta actgccacag aattaaatag cgtgtctgta aatgaatttg
     2521 ggcgttttgt aaggtgtata aaaactcgcc taaatcttcc ggcttaatgg ttttgaaatg
     2581 ttcaaccgtt gggctatcaa actctttcgc aatattggcg gtagggttaa acggtatcat
     2641 ttcacggtgt acggcaaaca tcataatttc attatgtttt tgaattgccc gttttaactt
     2701 ctctagtttg ccctcgtctt ggtattgctt gaatgccttg attgccattg gtgcggttat
     2761 ttgtgtgata ggcattcttc caaagtgcgg caagaggtta tttttaatta aggcgattac
     2821 atctttttgg tagttgtctg aaaaattctt cttggtcttg cggtgttcca gccacgccca
     2881 cgctacattt tcataagtgt tgctgatttc gttggccttt gcctgttcct gttgttggcg
     2941 gtgggtttga gggtcgatat tttgagccaa taatgcccta tattcttctc taatttctct
     3001 cgcttgtgct aaagaaatat cagggtaagc cccaatgctt aaatcattcc gcttttttgt
     3061 gaacggtctt tcatacctaa accgccacaa tttagaattt gaggtaataa caagtagtaa
     3121 gccgttaccg tctcttaatg ggctgtcttc cggtttcgct tttcttattt gggtatcgtt
     3181 taacggtgtg atttttctag ccatagtaaa cctttaggag tataaagcct aaatcttagg
     3241 agtatgattt tttgtttggg agtacaaaac taaaagcgat aaagccttat ttgaaaaggc
     3301 tctaaagtgg ttttaggagt acgaattttt gggggtattc cggcgtactc ctaaatatac
     3361 tcctaaaatc ttcggatttg attgtatcgt ataggtacgc aacggacaat aaaaaagcct
     3421 tgaaacattg attttcaagg ctttttaggt atcggttgaa ccgtatagga ttataatttg
     3481 gtggagctgg ggggatttga acccccgtcc gaaatttctc taccttcagt actacatgtt
     3541 tagtctagtc tttaatttcg cttattcaca gcggacagac acgcaatgaa taaactagct
     3601 ttatttaatt tagtgtttcg atcctaaagc ggcggcgtcc acacgatctc gttttgggtg
     3661 acttatcttt gccctcgtct tacgagcgga agctgaggag ataaggcttt aaagcaggtt
     3721 attaagctgc taaagcgtat tgttcgtcgt ttgcgactat tattttgcgg tttgtttacg
     3781 aggcctaccg cacctcgaca tgcaccttgg gcttcgccaa tcccgtcgaa tccaaaatca
     3841 gccccaaaag tagcggtatt ctaaaggaaa gcggtcgaat ttgcaaaaag ttttgcaagt
     3901 ggcggaactt agcggtattg aatagctcga aataaaatca tacaaatccc acaccagata
     3961 gatttaactc tgttataatg taaaccttgc tgagagtgaa gagaaataga atgaaaaaat
     4021 ttaacgagaa cgagattgaa gatgctgaaa ttatagatgc cgagccgatt gatgttgatg
     4081 agatagacga agaagaaaca tcggtagaag caatgcatcc ggctttggtt tccggctcaa
     4141 tgcctgtgcc gcagggcaat ttggatagct acatccgaat ggcaaatcag tatccgattc
     4201 tcaccgccga gcaagaaaaa gaactggcgg agcgttatta ttatgatgaa gacgttgagg
     4261 cggcaaaaca gctgatcctt tcccatatgc ggtttgtgat ccatattgct cgtggctatt
     4321 tgggctatgg tttaccgctg gcagatctga tccaagaggg taatatcggc ttgatgaaag
     4381 cggtaaaacg gtttgacccg aatgtcggcg tgcggttggt ttcttttgcg gttcattggg
     4441 tgaaagcgga aattcacgaa tatgtgctga aaaactggcg aattgtgaaa gtggcaacca
     4501 ccaaagctca acgtaaatta ttctttaatt tacgcaaaaa taaaaaccgc ttggcgtggt
     4561 ttaatgaaga agagatcaaa aaagttgccg atgatttagg ggtttcagtg gaagaagtac
     4621 gagaaatgga atctcgaatg accggacaag atttaggttt tgacctgcct gccggtgatg
     4681 acgatgaaga cagctacgct ccttctatgt atattgaaga tagcagctcc aatttcgccg
     4741 atgacttgga agacgaacaa cataatgggc aggcaaccgc tcaacttgcc tatgcgttag
     4801 ccacccttga tgaacgtagc caagacatca tcaaaacccg ctggttagat gacaataaag
     4861 ccacactgca agatttagcc gataaatata acatctcggc agagcgggta agacagcttg
     4921 aaaatgccgc attgaagaaa atcaaggaag cgatttcatt agacggctag aaatgcctac
     4981 aagcggtgat ttttttactc gctacgctcg cacttcgtgc cgtcggcaga gccaacgttc
     5041 aaacgcaagc gtttgtgcaa aattttacgc aaaaagcacc gcttgtattt tgactaaaaa
     5101 tgaggatact atgattaaca ttaccgataa acaaattgac ccaactttct accagcgtct
     5161 accagcgagc agacggcttt attaacgttg ccaacgctca tttaaaaaac attgccccaa
     5221 accaagtcag caatgcgatg ttgtttggtt gtgccagatt taacgcctat gttgcggcaa
     5281 gcaaggcgga atataaacag cagttggccg attccagaga agaagttatt caatattttg
     5341 tggagcaata caaagaaatg ctcactgcaa atttagatga atatatccaa aacttcgagc
     5401 gatatattga agggaaaaag gcggattaac cgcctttgtt ttgtggtcaa acttgtttta
     5461 caaaatcact taaaacttga ttaattctac tttgccaacc tttacctgaa gctctaaagg
     5521 cttcaagaac gcttttatca agacgtaacg aaaccaattc tttctttgaa ccagctggtc
     5581 tgccacgttt tttcattgca attgaaaact ctgatggcgt aaccgggcgt tcatcgtcat
     5641 cttttccgtc ccaaataaaa tcttggcttg ccggaatagc ttgaatagcc ataagtagct
     5701 gttttttctc taaattttta ttattcaaag tattgttgta agtattcata atatgcctct
     5761 ctttctgctt tagatgctcg ccgaaagcta attactctga tgtaatcttc cctttctgta
     5821 tatgcaagtg ataatacgag taaacgtcca aatgaatatg caaaagattg tattctaaat
     5881 tcattgttac gaataacttc aacatcaaat cgatagggcg atgagagaac ttcatctgca
     5941 tcggcaaaat ctaatccgtg cttggctaaa ttgattttgc gtttagtttc gtcccaagta
     6001 taaattttca taattattgt atatacataa aataaatcta acaagaacta aatttaattt
     6061 agcaaacagt aatttgaaaa tcagattaga aatacatttt ttcgatgcag atcgcaaaaa
     6121 taaaatgaaa tttaactgct tgaagagtct actgagccat tttccccgct accgttaaaa
     6181 aaccatctgc ggttttaaaa atttcaccga atccttgagc ctccaaaatt tggtcggaga
     6241 cttcacgaaa cgccccttca ccaccggctt tttgcaatac ccaatctgct ttttctttga
     6301 tgtaatcgag ggcgttacgg gttgcaatcg caatgccaca cacttcaaaa gccggtaaat
     6361 ctaaggtgtc atcaccaatg taagccgttt gctcaggggt aacaccggct tgctgcatta
     6421 gctcaaagca ggcggagcgt ttttccattt tgcctaacaa agcgagatca attttcagca
     6481 cctctaaccg tttgcggagc aaagccgtat cgccgcccga gagtactgcc actttaatgc
     6541 cacaactttg taacatctta acgcctaaac catcgtgaac gtggaaacgt ttcattactt
     6601 cgccttggtc ggtgtagtac attccgccat cggttagcac gccgtccaca tcggtaatca
     6661 ctaatttaat cttggtgaga tctaacattt ttttgcctca aatatggtat gattttacca
     6721 ttatagcttt tttacctccg tttaccaaag gaaccgaaat gtcgattgcc gagaatttat
     6781 cgcaaatcca tcaacaaatt cagcaaattt ccgaacaata tcaacgagat aacgtgcgtt
     6841 tgttagccgt ctccaaaact aaaccagttg aggcgattca agcggcgatt gaggcagggc
     6901 agcgtgcatt tggcgaaaat tatgtgcagg aagcggtcga aaaaatcgaa ttttttgcaa
     6961 atcgccccga attagaatgg cattttattg ggccacttca atcaaataaa accaaattgg
     7021 tcgctgccta ttttgattgg attcaaacgg tggatagatt aaaaattgcc gagcgtttaa
     7081 acgaacaacg cccagcggat aaagccccgc tcaacgtgct gattcaaatt aatatcagcg
     7141 atgaagcctc gaaatcgggc attgaaccag atgaaatgct accacttgcc caagcgattt
     7201 ctcaattacc gaatttgaaa ctgcggggct taatggccat tcccaaacca gaaagcgagc
     7261 cagagcaaca aaaagttgcc tttcgcaaaa tgcagctact ttttaaccgc ttgcaaacag
     7321 aatttgacga cattgatacc ctttcaatgg ggatgtcgga cgatatgcaa gcggcgattg
     7381 aatgtggctc gacaatggtg cggattggga ctgcgatttt tggggcgaga cgttagccag
     7441 ctgagttttt acataaagct gatacaagcg gtcaaattcg ccaaaaactt tgcaaattcc
     7501 accgcttgtt ttgtagccaa cagcggaaaa agtctgtata attacgcctc ctaatttctt
     7561 gactctcgcc agttttgatg ggcgagagtt tttattttga cttaacttaa agatattcca
     7621 atgcaaaatg tagatatttc aaaattgcgt aacattgcaa ttattgccca cgttgaccac
     7681 ggtaaaacca ccttggttga taaactttta caacaatcag gcacctttgg tgaaactcgt
     7741 ggtgacgttg atgagcgtgt aatggattcc aacgatcttg aaaaagagcg tggcattacc
     7801 attcttgcaa aaaatacggc aattaactgg aatggttatc acatcaatat cgtagatacc
     7861 ccagggcacg ctgacttcgg tggtgaggtt gagcgtgtac tttcaatggt agattctgtt
     7921 ctgttagtgg ttgatgcctt tgatggcccg atgccgcaaa cccgtttcgt cacgcaaaaa
     7981 gcgttcgctc acggtttaaa accaatcgta gtgatcaata aagttgaccg cccaggtgct
     8041 cgccctgatt gggtggtgga tcaagtgttc gacttgtttg taaatttagg tgcaagtgat
     8101 gagcagttag atttcccaat tatctacgct tcagccttaa atggtgtagc aggcttagaa
     8161 cacgaagacc tagcggcaga tatgacccca ctttatgaag cgattgtcaa acacgttcaa
     8221 ccaccacaag tggaattaga tgcaccattc caaatgcaga tttcgcaatt agactacaac
     8281 aactatgtgg gcgttatcgg tattggacgt atcaaacgtg gttcggtgaa acctaaccaa
     8341 gcggtaacga ttgtagatag cgaaggcaaa actcgccaag gtcgtatcgg tcaggtgttg
     8401 gggcatttag gtttacaacg ttttgaagca acagaagcct ttgctggcga tattgttgcg
     8461 attactggtt taggcgaatt aaatatttca gatactattt gtgatattaa cgcagtagag
     8521 gctttaccag cattaagcgt tgatgaacca acggtaacga tgttcttctg cgtaaacacc
     8581 tcgccattct gcggtcaaga aggtaaattt gtgacttcac gccaaattct tgagcgttta
     8641 aacaaagaat tagtccacaa cgtggcattg cgtgtggaag aaacttcgaa cccagacgaa
     8701 ttccgtgtgt caggtcgtgg cgaattacac ttatcggtat taatcgaaaa tatgcgtcgt
     8761 gaaggttacg agttagcagt gtctcgcccg aaagtaatct accgcgaaga agacggcaaa
     8821 aaacaagagc cgtttgagca agtgacgatt gatatcgaag agcaacacca aggttcagtg
     8881 atggaagcac ttggtatccg taaaggtgaa gtgcgtgata tgattccgga cggcaaaggt
     8941 cgtacccgtt tagagtatgt gatcccaagc cgtggcttaa tcggtttccg taacgaattt
     9001 atgacgatga cctcaggtac tggtttactt tactctacat tcagccacta tgatgacgta
     9061 aaaccgggcg aaatcggtca gcgtaaaaac ggtgtgttaa tttctaacgc aacgggtaag
     9121 gcattagcat acgcattatt tggcttacaa gagcgtggaa aattaatgat cgaccacggt
     9181 gttgatgtat atgaagggca aattatcggg attcacagcc gttcaaacga tttaaccgta
     9241 aactgcttac agggtaagaa attaaccaat atgcgtgcat caggtaaaga cgatgccatc
     9301 gttttaacca caccggtgcg tttcactcta gaacaagcgt tagagtttat tgacgatgac
     9361 gaattagtgg aagtaacacc acaatcggtg cgtattcgta aacgtttatt aactgaaaac
     9421 gaccggaaac gtgcaagccg tactacaacc agtaccagca cgcactaact gataaaaaag
     9481 agcgactgag gtcgctcttt tggttttaaa ttctccctac aaatataacg ccattaccac
     9541 cgcattttct ttgctaccgt ttggcgtggg gtaatagttt ttgcgggtgg taacttcatt
     9601 aaaaccgagt gaatcgtaga gttttctggc agaatggttg gattctcgca cttccaacca
     9661 cagggtggtg agtttccgtt gttgcagttg caaaattaat tcggaaagta accgcttgcc
     9721 aatgcctttg ccttgaaaat caggagcaac tgcaatgtta aataaggttg cttcatctag
     9781 cacgatatga ctaatggcaa aagccacaat tttgttttgc tctaccactt ttaaattcag
     9841 gtatttttca ccctgattat ttaataacgt cccctttgac cacggcacta aatgggcttg
     9901 ttgctcaatt tcaaataact gctcgaaatc ttccgcttga atttgctcaa tcacgctgtt
     9961 ctccaaattg tgccgaaaaa gtatcaattt gttgccaaag ttggcgtttg gtcgtagatt
    10021 tggctaaatc ttgccaagac tcgtttttcc aagcggtctg attagtaaaa ctttttgcca
    10081 ttttgaccgc ttgttcttcc gcttgaatca gccaaaagat cggcgaatgc tcaaacacca
    10141 aacgttgtga ttgttcgcca gtcagccatt gatattcgct gttttttaac tttaaagcac
    10201 gcagaatatc ggcaaagagc cggctgtgtt ggtgatcttc ctcacacact accaccaatt
    10261 ttaccgcatt gtttaggcgg atttgagcat cgccttttag cacttgggga ttggttagca
    10321 cccattgcga aatacccatt tcctgtaaca gtaaatcacg acgattcatt taaaacctca
    10381 tcaaattttg caaatagtat caaaactccg cttgaacaga aacttttttc aaaaaattca
    10441 agatttctgt acttaaattc ggtatattca cgctaaattt tttagataag gtaagaaaat
    10501 gctctcttta gaaagcgaag tacttgaacg ccatttggcg ttatttgaaa acaagtcggt
    10561 gttattattt ggcgatgtgc gagatgattt tgcccgccag ctgcaatctc gtgcggaaaa
    10621 ggttgcggta ttcagtagct attttgatta cgcacgccac catagtgagg ttgaatttgg
    10681 cttagaatgt ggtttgagtg ccgagttagc ggtgttttat tggacgaaga acaagcagga
    10741 atgccaattc caactgctac aatggctgtc gcaatgccaa gcagggcaag aaatgttgat
    10801 tatcggcgaa aaccgagctg gtgttcgttc ggtagaaaaa ctgctcgaac cgttcggcaa
    10861 tattgccaaa atcgactctg cacgccgttg tggtttatac cattttgagc tacagaccgt
    10921 gccgacattc gattgcaaaa aattctggaa atcctaccgc ttgcaagact tagatgtgtt
    10981 cgatctacca gcggttttta gctcggcgga gctggataac ggcaccaaat tattgctttc
    11041 aaccttcgac aaagcagaca ggctaaaagg caaagtgtta gatttaggct gtggggctgg
    11101 cgtgattggg gcaacgctca aacaacaatt tccgaaaatc aaactgacga tgagcgatat
    11161 tcacgcaatg gcgttggaat ccggcagacg tactttggcg gaaaatcagt tagaggggga
    11221 agtggtagca agtgatgtgt tctctcacat taatgaacgg tttgatttaa ttgtctccaa
    11281 cccaccattc cacgacgggg tcgataccgc ctaccgagcg gtggaggagt tgattttcca
    11341 agctaaaaat cacctaacca aaggcggtga gctgcgaatt gtcgcaaacg cattcttgcc
    11401 ttacccagat ttgctcgata aagccttcgg caaacacgac gtactggcaa aatccaccaa
    11461 gtttaaggtg tattccgcaa gaacataata ggcagaacaa acggttataa aaaagccggt
    11521 atttgcttaa aaaagtgctt gatatttctg ccggcttgac gtaaattctg ttcatactgt
    11581 tttggagtaa tgcaatgaat ttaaactaca ctttacatca ccaccacact ttaacctgat
    11641 atctttcggg catttggtgt gtgcggaaga taactcttcc gagcaagtgt agcaaatcaa
    11701 taaagatctc tcggaaggca actttcgaga gattttttta tagataaaat ttgggaataa
    11761 tatgaaaaac ccgattgaaa tttaccaaag ccaagacggc aaaacccaag tttccgtgca
    11821 gtttgataag gaaacggtgt ggctaagttt acagcaaatg gcagatattt ttggacgaga
    11881 taagtctgtt atttctcggc acttacgaaa tatctaccaa gaaggtgaac tggagcgtga
    11941 ggcaactgtt gcaaaaaatg caacagttca aattgaaggt gctagagaag tcgttcgcct
    12001 aaaagactat ctggtgaaag gctatgccct taacgaacgc cgtttgcagg aacgaggtat
    12061 cgagtttgag caggtgattg gtttgctcag tcaatacact ggttgtctta gccttgttgg
    12121 tggcagaaag tttgcctgaa caaaaagaac tgatgattaa actgattgaa cactttattt
    12181 tattaaaagc ataacaggaa aaaatatgag tattaatatt tcgccgttag cctccaaaaa
    12241 tctcactgaa tggaaactat tgtggcaaca atatttagat ttttatcaag tgaatttagc
    12301 agatgatatt attcaatcaa cttggcagaa agtttcagag agtgaaaatg taaagggatt
    12361 tggggctttt gttgataaca gaatggttgg ttttgttcat attgtgattc acccaaatac
    12421 ttggaattta actgaatgct gttatttgga agatttattt gttttagctg aatatcgcaa
    12481 ccaaggcata ggaaaagaat taattgaata tatttatcga tatgcacagg aaaaaggata
    12541 taaccgagta tattgggtaa cagataaaaa caatcatcaa gcac
//
LOCUS       DANLYI010000055        12507 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.055, whole genome shotgun sequence.
ACCESSION   DANLYI010000055 DANLYI010000000
VERSION     DANLYI010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 12507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12507
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.055"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            complement(313..495)
                     /locus_tag="PXD81_001136"
     CDS             complement(313..495)
                     /locus_tag="PXD81_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5537170.1"
                     /translation="MYQFGYLRTKIGTDTQRAMLWNCPTYVLIIPFLLNTSVKKQYHA
                     FWQKYHQHNNDVSFPY"
     gene            645..1685
                     /gene="selD"
                     /locus_tag="PXD81_001137"
     CDS             645..1685
                     /gene="selD"
                     /locus_tag="PXD81_001137"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020909986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:690..692,aa:Sec)
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HDL5537171.1"
                     /translation="MSEPIKLTQFSHGAGUGCKISPKVLGTILQTQLEQFVDPNLLVG
                     NETADDAAVYDLGNGQAIISTTDFFMPIVDAPFDFGRIAAANAISDIYAMGGKPIMAI
                     AILGFPVNKLPAEVAQQIVEGGRFACQQAGIALAGGHSIDSPDPIFGLAVTGIVPTER
                     VKRNASAVAGCELFLTKPLGIGILTTAEKKGLLKPEHQHLASEVMCQINLIGSAFSEL
                     PEVTAMTDVTGFGLLGHLSEVCQGSGLRAEVDFEDIQVLDGTKEYIALGAVPGGTNRN
                     FDSYGHLISAMTDEQKAILCDPQTSGGLLVAVLPQAVEKVQQIANRANVPLFHVGRLL
                     EMEQGKPRIEVI"
     gene            1685..2158
                     /locus_tag="PXD81_001138"
     CDS             1685..2158
                     /locus_tag="PXD81_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HDL5537172.1"
                     /translation="MFKPNVTMACVVHCKGKFLFVEEIEYGKRTLNQPAGHLEKDETL
                     LEGASRELFEETGIRAEMANLVKIYQWQAPRSQTDFLRFLFSVELEEWLEPNPQDADI
                     TQALWLTLAEFEDYIQQQGQCARSPLVVQSVKDYLSGERYPLSLLNAFEWARNGK"
     gene            2148..3356
                     /gene="ubiH"
                     /locus_tag="PXD81_001139"
                     /gene_synonym="visB"
     CDS             2148..3356
                     /gene="ubiH"
                     /locus_tag="PXD81_001139"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HDL5537173.1"
                     /translation="MENKTTTQFDVVIVGGAVTGSVLALALSSFTHHKISIAIVEKSL
                     PDYEQQGGFDARSIALAEGSLQKMGQIRPLAGSSLGEMISEIGTPIQQIQVADKGHFG
                     KTTLKASEQNVAQLGMVVELAKFGRKLTACLAQQANITLFCPNQVVGIERSQQHCGLS
                     LKNGEKLSCSLLVAADGIQSQIAKQCGVETVKVRDYAQSAIIANVELSESHQNQAFEY
                     FTPQGPFALLPLSGNMMSLVWCVKEPNELIQLSDSEFLARLQQQFGWKLGQFVRVSKR
                     FVYPLILQKAQSHIHHRLAVVGNAAQMLHPVAGQGFNLGLRDLTTLAELVALAFNQGK
                     DIGEFSLLSTFENERTQDQARMINATSGLISLFCCEMLPVQVVRNLGLLTVSHSKIAR
                     DFVANQALGW"
     gene            3619..4851
                     /locus_tag="PXD81_001140"
     CDS             3619..4851
                     /locus_tag="PXD81_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-xanthine permease"
                     /protein_id="HDL5537174.1"
                     /translation="MTTENMSTPKQAFIGLQMLFVAFGALVLVPLITGLDPNVALLTA
                     GVGTLIFQICTKRQVPVFLASSFAFIAPIQFGVQQWGIPVTMGALLCSGFIYMALSAL
                     VKAKGVNILHKYFPPIVIGPVIMVIGLGLAPVAVDMATGKSANIAYNTAIIVSMITLV
                     TTLIVAVFAKGLLKLVPILAGLTVGYILSVFMGLVDFTKVVEAPWFSLPKVSAPEFKL
                     DAILYLLPIALAPAVEHLGDVMAISQVAGKDFMKKPGLHRTLLGDGLATSAAAALGGP
                     PNTTYSEVTGAVMLTKNFNPKIMTWAACWAIAFSFCGKIGAFLATIPTFVMGGIMMLL
                     FGAVAVVGINTLIKAKVDLSIPRNLCIASVVMTFGIGGMLINIGEFSLKGISLCALVA
                     IVLNIVLPKEKVEDSAAH"
     gene            4918..5904
                     /locus_tag="PXD81_001141"
     CDS             4918..5904
                     /locus_tag="PXD81_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HDL5537175.1"
                     /translation="MQKLTFSPPLLNTPCPWCSELESLRELYACEYTGAVTTRTSMPQ
                     PYPHDWAKNQYVLFDSNAQKTASVNTQDATSLQTASLNTIGLSPNNLDTTISFVRTIS
                     NELTAPSSKPFIISVFGSPEEVGECYEKIVAFQTEVKMPLAMEINISCPNIPGEISPA
                     YSAEELSHYLHALQTSLKKLAQGDNTLPIGIKIPPFTYQNQYDELIKGLLQAVENGEN
                     LPISFITSTNTLGSSLVLNAGKAVLNSEVGTGVGGMAGTAIHALSLGNVYTIRQMLDQ
                     EPKLKHLQIIGVGGVNDKAGFERMKSVGADFVGLATALGVKGIGVFKEILSE"
     gene            complement(5945..6355)
                     /locus_tag="PXD81_001142"
     CDS             complement(5945..6355)
                     /locus_tag="PXD81_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005625316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2251 domain-containing protein"
                     /protein_id="HDL5537176.1"
                     /translation="MLYSVLTAHILLNEPYRNGAHSTQHEHLVVMFEIDEESGYFYAM
                     DLHQTEQPVVDSLFVFRVSDIEQNSLKEPRRLEICWSEDGYQAFLLINGYPHAVFDFR
                     QFVGYNHTKFPMPELGSMWVHKETTNELVEKWLG"
     gene            complement(6421..7731)
                     /gene="eno"
                     /locus_tag="PXD81_001143"
     CDS             complement(6421..7731)
                     /gene="eno"
                     /locus_tag="PXD81_001143"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HDL5537177.1"
                     /translation="MAKIVKVIGREIIDSRGNPTVEAEVHLEGGFVGLAAAPSGASTG
                     SREALELRDGDKGRFLGKGVLKAVSAVNNEIANALVGKDGSAQAEIDQIMIDLDGTEN
                     KSKFGANAILAVSLATAKAAAASKGLPLYAYIAELNGTPGVYSMPLPMMNIINGGEHA
                     DNNVDIQEFMIQPVGASTLKEALRIGAEVFHNLAKVLKAKGLNTAVGDEGGFAPNLAS
                     NADALACIKEAVEKAGYVLGKDVTLAMDCASSEFYDKERNVYDMKGEGKTFTSQEFTH
                     YLEGLCKEYPIVSIEDGQDESDWEGFAYQTKVLGDKVQLVGDDLFVTNTKILKNGIEK
                     GIANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQ
                     IKTGSMSRSDRVAKYNQLIRIEEALERAGTPAPFNGRKEIKGQA"
     gene            complement(7922..9100)
                     /gene="rlmC"
                     /locus_tag="PXD81_001144"
     CDS             complement(7922..9100)
                     /gene="rlmC"
                     /locus_tag="PXD81_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HDL5537178.1"
                     /translation="MSSLLHCSHFERGDCTSCQWIEKPYSTQLAAKEADLKRLILPFI
                     LQNKTEILPLVGSPISQFRNKAKMVVSGSVERPILGILKDQNDAQSGVDLTDCLLYPS
                     SFHRLFPILKDFIARAGLVPYNISKKKGELKYILITESQYNQSFMLRFVVRSEAKRAL
                     VERELPELLEKLPPNSVVSLNIQPQHAALLEGETEIFLTEQKVLEEHFNGIPLFIRPQ
                     GFFQTNPVVASQLYHTAQSWIEHLPIAKLWDLFCGVGGFGLHCANALQAKNSNVELTG
                     IEISASAIASATKSAEKLGLKQVKFASLDSAQFALNEAGSPPDLVIVNPPRRGIGKLL
                     AEFLNQLGSPYLIYSSCNAVSMANDFTFLTNYELHKVQLFDMFAHTAHYETLTFLVKK
                     "
     gene            complement(9093..10130)
                     /gene="nagZ"
                     /locus_tag="PXD81_001145"
     CDS             complement(9093..10130)
                     /gene="nagZ"
                     /locus_tag="PXD81_001145"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HDL5537179.1"
                     /translation="MLLIDIKDKELTQEEVEMLEHPLVSGLILFTRNFYDKAQIQALV
                     KSIRQRVKKPLLITVDQEGGRVQRFREGFTKLPAMQSFNQLATTPEQAMQLAKETGWL
                     MAAEMFALDIDLSFAPVLDLGHECKAIGDRSFGQEPAAILPVAEAFIDGMREIGMATT
                     GKHFPGHGHVLADSHLETPFDDRPKERIFNHDILPFKQLIAKGKLSAIMPAHVVYTQC
                     DSQPASGSSYWLKEVLRKQLNFNGVIFSDDLGMKGAGFMGNFVERSEKAIQAGCDLLL
                     LCNEPEGLVQVLDGLKYQPTQAQKERRLSLMKRKTVTWRELENSSRYQQTQAELTALQ
                     NNWLEWKEANV"
     gene            complement(10256..10987)
                     /locus_tag="PXD81_001146"
     CDS             complement(10256..10987)
                     /locus_tag="PXD81_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005604847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HDL5537180.1"
                     /translation="MFDWITNPEAWVALFTLTGLEIVLGIDNIIVISILVSRLPIEQR
                     QSARIIGLGLAMGTRILLLLSLAWMMRLVEPLFTIGNNPISGRDLILLIGGLFLIVKS
                     AMELKESISAESHEEKSESIKKASFFMVLIQIAIFDIIFSLDSVITAVAMADDIPVMV
                     IAIIIAVAVMMLAAKTIGDFVDNNPTIKNLALAFLILIGIVLVGEGFDIHIPKAAVYT
                     AMGFSVVVEMLNIKMRRNQAKLEQA"
     gene            complement(11076..11756)
                     /gene="mutH"
                     /locus_tag="PXD81_001147"
     CDS             complement(11076..11756)
                     /gene="mutH"
                     /locus_tag="PXD81_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005610420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HDL5537181.1"
                     /translation="MQLSTFSPTEQALLSKAEWLAGFTLGEIAEMLHIPIPADLKRDK
                     GWVGTLIETALGAKAGSKAEQDFAHLGIELKTIPVNQKGLPLETTFVSLAPLTQNNGI
                     IWETSHVKHKLSRVLWIPVEGERKIPLAERHIGQPILWSPTITQESRLQQDWEELMEL
                     IVLGELHKINATLGEVLQLRPKGRNNRSITSAINAKGEIVQSLPLGFYLRKHFTAEIL
                     QNFLHCPL"
     gene            complement(11848..12483)
                     /gene="lexA"
                     /locus_tag="PXD81_001148"
     CDS             complement(11848..12483)
                     /gene="lexA"
                     /locus_tag="PXD81_001148"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repressor LexA"
                     /protein_id="HDL5537182.1"
                     /translation="MRKHLTARQQEIFDFVKNHIETTGMPPTRVEIAREIGFKSPNAA
                     EEHLKALARKGYIEMLSGTSRGIRILVEDENQAANDEDEGLPLIGKVAAGTPILAVEH
                     VENHYPVNGAMFNPSADYLLKVNGNSMEKIGILDGDLLAVHKTNTARNGQVVVARVND
                     EVTVKRLEKKGELIYLHPENDELKPIVVNPAQEYIEIEGIAVGVIRSNAWM"
BASE COUNT         3498 a         2543 c         2795 g         3671 t
ORIGIN      
        1 aggaaaaatg tgctgtggca gaacaccgat ggcaacctta cttgatggaa aacggatttg
       61 ggtggaaaag aatttaagct caaattaatc tgacagacac ggtaatttaa aacgggagac
      121 tgtcagatca agtctgatct tctacaacta agccttctca gaataaataa attttcacaa
      181 gcggtgcaac tttgcaaaaa cttcggtatt atgtagcagt tgcacaaaac cccaaattca
      241 ttaaaaccgc ttaaaaatga aatgccctaa actgattttt cagtctaggg catttattat
      301 cacatattat ggttaatagg gaaaactcac atcattgtta tgctggtgat atttttgcca
      361 aaatgcgtga tattgttttt tcaccgatgt gtttaataaa aacgggatta tcaagacata
      421 ggtcgggcaa ttccatagca ttgcacgttg tgtatccgtg ccgattttgg tgcgtaagta
      481 accaaactga tacattagac ccaatccaac cgtcacctat tctaatagca taataactaa
      541 ggtcgcatca ttaaattaaa agctcatttt ttgcaaaaaa tttcccaaat tccaccgctt
      601 gttggcgtac aataggcata attttgattt acgatggagg cagaatgtct gaaccgataa
      661 aacttacgca gttcagccac ggggcgggtt gaggctgtaa aatttcgcct aaggtgttag
      721 ggacaatttt acaaactcaa ttagaacaat ttgtcgatcc gaatttattg gtaggcaatg
      781 aaacggcgga cgatgctgcc gtctatgatt tagggaacgg gcaggccatt atcagcacca
      841 ccgatttctt tatgccgatt gtggacgccc cgtttgattt cgggcgaatt gctgctgcca
      901 atgcgattag cgatatttat gcgatgggtg gcaagccgat tatggcgatt gcgattctgg
      961 gctttccggt gaataaattg ccggctgaag tggctcaaca aattgtcgaa ggtgggcgtt
     1021 ttgcgtgcca gcaagccggt attgccttgg cgggcgggca ttcgattgat tcgcccgatc
     1081 cgattttcgg cttggctgtt accggcattg tgccgaccga acgggtgaaa cgcaatgcct
     1141 ctgcggtggc tgggtgtgag ctgtttttaa ccaagccttt ggggattggc attctaacca
     1201 cggcagagaa aaaaggtttg ttgaagcctg aacatcaaca tctggcaagc gaggtaatgt
     1261 gccaaatcaa cttgattggt tcagcgtttt ccgaactgcc tgaagtcacg gcaatgactg
     1321 atgtaaccgg ttttggctta ctcgggcatt taagcgaggt gtgtcaaggc tcgggcttac
     1381 gggcggaggt tgattttgag gatattcaag tccttgatgg cactaaggag tatattgcat
     1441 tgggagcagt tccgggtggg acaaatcgca atttcgacag ctatgggcat ttgatttcgg
     1501 caatgacgga cgagcaaaaa gcgattttgt gcgatccgca aacctctggc ggtttattag
     1561 tggcggtgct gccacaagcg gtcgaaaaag tgcaacaaat tgcaaatcgg gcaaatgtgc
     1621 cgttattcca cgttggcaga ttgctggaaa tggaacaggg caaaccacgg attgaggtaa
     1681 tttgatgttt aaaccaaatg taacaatggc ttgcgtggtg cattgtaagg gaaagtttct
     1741 gtttgtggaa gaaattgaat atggcaaacg tacccttaac cagccggcag ggcatttaga
     1801 aaaagatgaa accttgctgg agggggcaag ccgtgagctg tttgaggaaa ccggtattcg
     1861 ggcagaaatg gcaaatttgg tgaaaattta tcaatggcaa gcccctcgtt cacaaaccga
     1921 tttcttgcgt tttctgtttt cggtggaact ggaagagtgg ctggagccga atccgcaaga
     1981 tgcggatatt acccaagccc tttggcttac tttagcagaa tttgaagact acattcagca
     2041 acaagggcaa tgtgccagaa gcccgttggt ggttcaatcg gtcaaagatt atttaagcgg
     2101 tgagcgttat ccattaagtt tgttgaatgc gtttgaatgg gcgagaaatg gaaaataaaa
     2161 caacaaccca atttgatgtg gtgattgtgg gcggggcagt gaccggctcg gtgctggcat
     2221 tggcgttaag tagttttact catcacaaaa tctcgattgc gatagtggaa aaatcgctgc
     2281 cggactatga acagcaaggc ggttttgatg ctcgcagtat tgccttagcc gaaggcagtt
     2341 tgcaaaaaat ggggcaaatc agaccgcttg caggctcaag tttaggcgag atgattagcg
     2401 aaatcggcac gccgattcag caaattcaag tagcggataa agggcatttt ggtaaaacga
     2461 ccttgaaagc aagcgagcag aatgtggctc aactgggtat ggtggtggaa cttgcaaaat
     2521 tcggcagaaa attgaccgct tgtcttgcac agcaagctaa tattacgctt ttttgcccga
     2581 atcaagtggt tggcattgag cgttcccaac aacattgtgg attgagcttg aaaaatggtg
     2641 agaaactaag ctgttccttg ctggtggcgg cagatggtat tcaatctcag attgcaaaac
     2701 agtgcggtgt ggaaaccgta aaagtaaggg attatgcaca atcggcaatt atcgccaatg
     2761 tggaactgag tgaatctcac caaaatcaag cctttgaata ttttacgcct caaggccctt
     2821 ttgctttatt gcctttgagc ggaaatatga tgtcgttggt gtggtgtgtg aaagagccga
     2881 acgagttaat ccaactttca gacagtgaat ttttagcccg tttgcaacaa caatttggtt
     2941 ggaagttagg gcaatttgtt cgagtgagca agcggtttgt ttatccgctc attttgcaaa
     3001 aagcccagtc gcatattcac caccgcttag cagttgtcgg caatgccgca caaatgcttc
     3061 acccggttgc tgggcaaggt ttcaacttag gcttgcgtga tttgactacc ttagcggaat
     3121 tagtggcttt ggcgtttaat caagggaaag atataggcga attttcgctc ttatcaactt
     3181 ttgagaatga gagaacgcaa gatcaagcca gaatgataaa tgctacaagc ggtctgattt
     3241 cgcttttttg ttgcgaaatg ttaccagttc aggttgtgag gaatttaggg ttattaacgg
     3301 tttcccattc aaaaatagcc agagattttg ttgctaatca ggcattaggt tggtaaaaaa
     3361 cacaaaagca gcaattttct atttgacact gtttttaaaa aggcgtatta tccaacccgt
     3421 ttaaaaatct caccaaagcc ttgaatgcaa attcaaggct ttttcctttt tagtgaaagc
     3481 ctatgaattt tgtttgccgt aaaatcggca aaaaaatttt gcctaaaatt cgcaatcgtt
     3541 tgcgaaatag gtttatagcg ttgttttcat ctgggtaact gcaatggtta catcgcaata
     3601 catcaaagag agtaaaaaat gaccacagaa aatatgagta cgccaaagca ggcgtttatc
     3661 gggctgcaaa tgctgtttgt tgcatttggg gcgttagtat tagttccgct gattaccggt
     3721 ttagatccta atgttgccct tcttaccgcc ggtgtcggta cgttaatctt ccaaatttgt
     3781 actaaacgtc aagttcctgt tttcctcgca tcgagtttcg cttttatcgc tccaatccaa
     3841 ttcggtgttc aacaatgggg aattcccgtc acaatgggag cattgctctg ttccggtttt
     3901 atttatatgg cattatctgc tttagtgaaa gcgaaagggg tgaatattct gcataaatac
     3961 ttccctccaa tcgtgattgg accggtaatt atggtgattg gtttaggtct tgctcctgtt
     4021 gcggtggata tggcaacggg taaatcggcg aatattgcct ataacaccgc tattattgtt
     4081 tcaatgataa cgctggtaac tacgctgatt gtggcggtgt ttgcgaaagg gctattaaag
     4141 ctcgtgccaa tattagcagg tttaacggtg ggatatatct tgtctgtttt tatgggattg
     4201 gtggatttta ctaaagtggt tgaagctcct tggtttagtc taccgaaagt ttccgcaccg
     4261 gaattcaaat tagatgcaat cttgtattta ttacctatcg cacttgctcc ggcagtcgaa
     4321 cacttaggcg atgtaatggc aatcagccaa gttgccggta aagattttat gaaaaaaccg
     4381 gggttacacc gcacgctttt aggtgatggt ttagcaactt cagcagctgc ggcattaggt
     4441 ggcccaccaa atacgactta ctctgaagta accggtgcgg taatgctaac caaaaacttc
     4501 aatccgaaaa ttatgacttg ggctgcgtgc tgggctatcg ctttctcatt ctgcggaaaa
     4561 atcggggcat tccttgccac tatccctact tttgtgatgg gcggtattat gatgttgctg
     4621 ttcggtgcag ttgcggttgt ggggatcaat actctaatta aagctaaagt tgatttaagc
     4681 attccacgta atctttgtat tgcttctgta gtaatgacat tcggtattgg cggtatgtta
     4741 attaacatcg gcgaattctc attaaaaggg atcagcttgt gtgcattagt cgctattgta
     4801 cttaacattg tattaccaaa agaaaaagta gaagattctg ctgctcatta atcaaaggac
     4861 gctgtggcgt cctttttgct aataaccctc tatttgtaaa taaaaaggaa taacactatg
     4921 caaaaactca cattttcacc accactttta aatacgcctt gcccttggtg ttcggagtta
     4981 gagagcttgc gtgaacttta tgcgtgtgaa tataccggtg cggtaacaac ccgtacttca
     5041 atgcctcaac catatccaca cgattgggcg aagaaccaat atgtactctt tgattccaac
     5101 gcacagaaaa cagcaagtgt gaatactcaa gatgcaacta gcttacaaac agcaagcctt
     5161 aacaccatcg gtttaagccc aaataatctg gataccacca tcagctttgt acgcaccatt
     5221 tcaaatgagc tgacagcgcc atcaagcaaa ccatttatta tttcggtgtt tggttcaccc
     5281 gaagaggtgg gggaatgtta tgaaaaaatt gttgctttcc aaactgaagt gaaaatgcct
     5341 ttggcaatgg agattaatat ctcttgcccg aatattccgg gtgaaatttc gcctgcttat
     5401 tccgcagaag aattatcaca ttacttgcac gcactacaaa cttcgctaaa aaaactggct
     5461 caaggtgata atactttacc aatcgggatt aaaattccac cttttactta ccaaaatcaa
     5521 tatgatgaat taatcaaagg cttattacaa gcggtcgaaa atggcgaaaa tttaccaatt
     5581 tcctttatta cttcaaccaa tacgcttggc tcatcgcttg tcttaaatgc tggcaaagcg
     5641 gtattaaatt cagaagtggg aacgggcgta ggcggaatgg caggaacggc aatccatgca
     5701 ctttctttgg ggaatgttta cacgattcgc caaatgttag atcaagagcc taagctcaaa
     5761 catcttcaaa ttattggtgt gggcggcgtg aatgataaag ccggctttga gcgaatgaaa
     5821 tcggttgggg cagactttgt ggggttagct accgcattag gggtgaaagg aatcggcgtt
     5881 tttaaagaga ttctgtctga ataatgaaga ggagggcgtg cattgcacgc ccgaataaat
     5941 gaaattagcc taaccatttc tccactaatt cattagtggt ttctttatgt acccacatac
     6001 tgccaagttc cggcattggg aatttggtgt ggttgtagcc gacaaattgg cggaaatcaa
     6061 acacggcgtg cgggtagccg ttgatcagta aaaaggcttg atagccgtct tcactccagc
     6121 aaatttctaa acggcgaggt tcttttaatg aattttgctc aatatcgctg acccgaaaca
     6181 caaataaact atccactacc ggctgttcgg tttggtgtaa gtccattgca taaaagtagc
     6241 cgctttcttc atcaatttca aacatcacta ctaaatgttc gtgctgggtt gaatgagcgc
     6301 cattacggta aggttcgttc agtagaatat gagcagttaa aacagagtag agcattaaat
     6361 tatccttata agtaaaatgc caccgatttc tcagtggcat ttaaatattt aatcaggcaa
     6421 ttacgcttga cctttaatct ctttacggcc gttgaacgga gccggtgtac cagcacgctc
     6481 taacgcttct tcgatacgga ttaactggtt gtatttcgct acacggtctg aacggctcat
     6541 tgaaccggtt ttgatttgac ctgctgctgt acctactgct aaatctgcaa tggttgcatc
     6601 ttcagtttcg cctgaacggt gagagataac tgcggtgtaa cctgcatctt tcgccatttt
     6661 gattgcagct aaagtttcag ttaaagaacc gatttggttg aatttgatta agattgagtt
     6721 tgcgatacct ttctcaatac cgtttttcaa gattttggta ttggttacga ataagtcatc
     6781 accaactaat tgtactttgt cgcctaatac tttagtttgg tatgcgaaac cttcccagtc
     6841 agattcgtct tgaccatctt cgatagacac gattgggtac tctttgcata agccttctaa
     6901 gtagtgagtg aactcttgtg aagtaaaggt tttgccttcg cctttcatat cgtacacatt
     6961 gcgttctttg tcgtagaatt cagatgatgc acagtccatc gctaaagtta cgtctttacc
     7021 taacacatag cctgcttttt ctaccgcttc tttaatgcac gctaatgcgt ctgcgtttga
     7081 agccaagttt ggtgcgaaac caccttcgtc acctacagca gtatttaagc ctttcgcttt
     7141 taatactttt gcaagattgt ggaatacttc agcaccgata cgaagagctt cttttaatgt
     7201 agaagcacct accggttgaa tcataaattc ttgaatgtcc acgttgttgt cagcgtgttc
     7261 accaccattg atgatgttca tcattggtaa tggcattgag taaacacccg gtgtgccgtt
     7321 taattcagca atgtatgcgt aaagcggtaa gccttttgaa gcggctgctg cttttgctgt
     7381 tgctaaagaa accgctaaga tagcgtttgc accgaatttt gatttgtttt ctgtgccatc
     7441 taaatcgatc ataatttggt cgatttcagc ttgtgcagaa ccgtctttgc ctactaatgc
     7501 gtttgcaatt tcgttattta cggcagaaac cgcttttaat acacctttac ctaagaaacg
     7561 acctttgtcg ccatcacgta attctaatgc ttcacgtgaa ccggtagaag cacctgatgg
     7621 agcggctgct aagcctacaa aaccaccttc taagtgaact tctgcttcta cagttgggtt
     7681 accacgagag tcgatgattt cacgaccgat tactttaaca attttagcca ttgttgatgt
     7741 cctctaatat acgagttaat agaaaaaaga ataggtatat agtaaagcga ttttctgctt
     7801 ttgtcttgga aaaactgatt gagtggcaga aaatttgcca ctaatttgat ctaagactat
     7861 attcgcctga gattatagca acctttgcaa aaaacggcaa aaatttgccc gcttgtaatg
     7921 cttatttttt gactaaaaaa gtcaaggttt cataatgtgc tgtgtgagca aacatatcaa
     7981 acagctgcac tttgtgtagt tcgtaatttg tcaaaaatgt aaaatcgttc gccattgaga
     8041 cggcattaca actggagtaa atcaaataag gtgagcctaa ttgatttaaa aattctgcta
     8101 atagtttgcc gataccacgt cgaggtgggt tcacaatcac taaatcgggt ggagaaccag
     8161 cttcatttaa agcaaattgt gcagaatcta atgaggcaaa ttttacctgc tttaggccga
     8221 gtttttcagc cgatttggtc gcacttgcaa tagctgaggc tgaaatctca atgcctgtca
     8281 gctccacgtt cgagttcttc gcttgcaagg cgttggcaca atgtaagccg aaaccaccga
     8341 caccgcagaa caagtcccac aatttagcaa taggtaaatg ctcaatccag ctttgtgctg
     8401 tgtggtaaag ctggcttgct accacagggt tagtttggaa aaagccctga gggcgaataa
     8461 atagcggtat gccgttaaaa tgttcttcca gcactttttg ttcggtgagg aaaatctcag
     8521 tttcgccttc taaaagagca gcgtgttggg gctgaatatt cagactaact actgagttgg
     8581 gtggcagttt ttccaacagt tcgggcaatt cccgttcaac caatgcacgc tttgcttcac
     8641 tacgaaccac aaaacggagc ataaatgatt gattatattg gctttcggta atcagaatat
     8701 attttagctc accttttttc ttactgatgt tatacggcac taaaccagct cgagcgataa
     8761 aatctttcaa aatcggaaaa agtctatgaa atgaggacgg gtagagcaga cagtcggtta
     8821 aatccacccc gctttgagca tcattttgat cttttaaaat gcctaaaatc gggcgttcta
     8881 cactgccgga gaccaccatt ttcgctttgt tacgaaattg cgaaattggc gagccgacaa
     8941 gcggtaagat ttccgtttta ttttgcaaaa taaacggcaa aatcaaccgc ttgagatcgg
     9001 cctctttggc ggctaattgc gtgctgtatg gcttttcaat ccactggcac gaagtacagt
     9061 cgccacgctc aaaatgcgaa caatgcaaca agctagacat tcgcttcctt ccattccagc
     9121 cagttgtttt gtaaggccgt taattcagcc tgagtttgtt ggtagcgaga gctgttttcc
     9181 agctctcgcc aagtcacggt tttgcgtttc attaaagaaa ggcgacgttc tttttgtgct
     9241 tgagtcggct gatatttcag cccgtctaac acttgcacca ggccttcagg ctcgttacaa
     9301 aggagcaata aatcacagcc ggcttggatt gctttttcag aacgctcgac aaaattgccc
     9361 ataaagcccg cacctttcat tcccaaatcg tcagagaata tcacaccgtt aaagttcagc
     9421 tgtttacgca atacctcttt aagccaatag cttgaaccgc ttgccggctg gctatcacat
     9481 tgggtgtaaa cgacgtgggc aggcataatg gcagagagtt ttcctttggc aataagctgt
     9541 ttgaacggca gaatatcgtg attaaaaatc cgctctttcg ggcgatcatc aaacggggtt
     9601 tctaaatgcg aatcggctaa aacgtgtcca tgaccgggga agtgtttgcc ggtggtcgcc
     9661 ataccgattt cacgcattcc gtcaataaag gcttcggcaa caggcaaaat cgctgctggc
     9721 tcttgcccga atgagcgatc gccaatcgct ttgcattcgt gtcctaaatc cagtaccgga
     9781 gcgaaactga ggtctatatc gagggcaaac atttctgctg ccatcaacca gccggtttct
     9841 ttcgctaatt gcattgcttg ctctggcgtt gtggcaagtt ggttaaaaga ttgcattgcc
     9901 ggcagtttag taaagccttc acgaaagcgt tgcacccgac cgccttcttg gtcgacagta
     9961 ataagcaacg gttttttcac ccgctggcga atggatttca ctaatgcctg aatttgtgct
    10021 ttgtcgtaga aattacgggt gaataaaatt agcccggaaa ctaaagggtg ttccagcatt
    10081 tcgacttctt cttgagtcag ttctttatct ttaatatcaa tcagtaacat aattttcgtt
    10141 gagctatttg caaaaaaatg agcagatatg accgcttgtt gtgcctattc tacattaaaa
    10201 agcccttgat gttgatcaag ggcgaatgat taagggcata aatgagacag agcaattatg
    10261 cttgttctaa tttagcttgg ttacgacgca tttttatgtt gagcatttca accacaaccg
    10321 agaagcccat tgccgtataa accgctgctt ttggaatatg aatatcaaaa ccttcgccga
    10381 ctagtacaat accgattaaa atcaagaacg ctaaagcgag atttttaatg gttgggttgt
    10441 tatctacaaa gtccccaatg gttttcgctg ctaacatcat taccgcaact gcaatgataa
    10501 tcgcaataac cattaccgga atatcatctg ccattgccac agcagtaatg actgaatcaa
    10561 gggagaaaat aatatcaaaa attgcaattt gaattaacac cataaaaaag ctggcttttt
    10621 taatggattc gcttttttct tcgtgagatt cggcagaaat ggattctttc agctccattg
    10681 cacttttaac aatcaggaat aatccaccga tgagtagaat gagatctcta cctgaaattg
    10741 gattgttgcc aatagtgaaa agaggctcga ccaaacgcat catccaagca agcgagagaa
    10801 gcaataaaat acgggttccc attgccagcc ccaagccgat aattcgggca gattggcgtt
    10861 gttcaatagg taaacgggaa acgagaatac tgatgacgat gatgttatca atgcctaata
    10921 cgatttcaag cccggtaagt gtaaagagcg caacccaagc ttcaggattt gtgatccagt
    10981 caaacataaa aatgtccttt taaaaataag ggattaagtg atattttcta tcaaatagaa
    11041 ttaaagccga catcttatag agtgtgagga caaatttaaa gggggcaatg caaaaagttt
    11101 tgtaaaattt cggcggtaaa atgtttacgc aaatagaaac caagagggag agattgaaca
    11161 atctcccctt tagcgttgat agcagaggtg atagagcggt tatttctacc tttgggtctg
    11221 agttgtaaca cttcgcctaa ggttgcatta attttatgca gttcacctaa cacaatcaac
    11281 tccattaatt cctcccaatc ttgctgtagg cgggattctt gtgtgatagt cggagaccat
    11341 aaaatcggct gaccgatatg gcgttcggca aggggtatct ttcgttcccc ttcaaccgga
    11401 atccaaagca cacgggaaag cttatgttta acgtgggagg tttcccaaat aataccgttg
    11461 ttttgggtta aaggagcaag gctgacgaat gtggtttcca gcggtaatcc tttctgattg
    11521 accggaatgg ttttcagctc aatgcctaaa tgtgcaaaat cctgttcggc tttactgccg
    11581 gctttcgcac ctaaagcggt ttcaatcaga gtaccgaccc agcctttatc tcgtttcaga
    11641 tctgctggta tcggaatatg aagcatctcg gcaatttcac ctaaagtgaa ccctgccagc
    11701 cattccgctt tgcttaataa tgcctgctcg gttggagaga atgtagataa ttgcattgca
    11761 cttagttaat tagactaaac tggcaagcgg tcaaatttag gcagaaattt gcaaaaaatt
    11821 actaaaaaat aaccgcttgt gaggaaatta catccaagca ttactgcgaa taacgcctac
    11881 tgcaatgcct tcaatttcaa tatattcttg agcagggttt actacaatcg gttttaattc
    11941 atcgttttca gggtgtaagt aaattaattc acctttcttc tctaagcgtt taacagtcac
    12001 ttcatcattt acccgagcaa ccaccacttg cccgttacga gcggtattgg ttttatgaac
    12061 ggctaatagg tcaccgtcta aaataccgat tttttccatt gaattgccat ttactttgag
    12121 taaataatca gcacttggat taaacatcgc accattcacc gggtaatggt tttcaacgtg
    12181 ttcaaccgct aaaataggtg tgccagcagc tactttgcca atcaacggta agccttcatc
    12241 ttcatcgttg gcagcttggt tttcgtcttc gactaaaatg cgaattccac gagatgtacc
    12301 tgataacatt tcaatatagc ctttacgagc aagggctttt aagtgttctt ctgccgcatt
    12361 aggtgattta aagccgattt cacgggctat ttctactcgg gttggtggca taccggtggt
    12421 ttcaatatgg tttttgacga aatcaaaaat ttcttgctga cgggcagtta gatgtttacg
    12481 cattttaagt taatcctcac tgtcttt
//
LOCUS       DANLYI010000056        12270 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.056, whole genome shotgun sequence.
ACCESSION   DANLYI010000056 DANLYI010000000
VERSION     DANLYI010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 12270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12270
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.056"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            144..803
                     /locus_tag="PXD81_001149"
     CDS             144..803
                     /locus_tag="PXD81_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009875387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HDL5537183.1"
                     /translation="MFNISKQQVLDAFHFRSACRSYDPNKKINKEDMDYILELGRLSP
                     SSVGSEPWKFLVLQNPETRQKIAPVSWGIKHPMEEMSHLVVILTKKNVRYDSVYMRDM
                     LAKRGLTYEQIEASLVRYKSFQEEDMKLLSERELFDWSTKQTYIALGNMMTGAALIGI
                     DSCPIEGFHYDTVNQILSDEGLFDLNEYGVSCMITFGYRNKEIKKKSRKPTEEVIAWI
                     E"
     gene            complement(863..2188)
                     /locus_tag="PXD81_001150"
     CDS             complement(863..2188)
                     /locus_tag="PXD81_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HDL5537184.1"
                     /translation="MLIFNLIVFAAILFLLAKIYKSTQKLGQTVFIGLFLGLASGAFL
                     QTFYEKATVDATLDWINLIGNGYVRLLQMIVMPLVFVSILSAITRLKEAGSLGKISFS
                     VLSVLLVTTAIAAAIGIALVYLFDLSAEGLVAGSRELAAQEKVVGRVEQVSNLSVPSM
                     LLSFIPKNPFLELTGANPTSIINTVIFSALLGIAALSLGKEDKALGERIAQGVETLNK
                     LVMRLVRFVIRLTPYGVFALMIKMAATSQWSDIVNLGSFIVASYIAIGLMFIVHGILL
                     FVAKINPLDYYKKVLPTLSFAFTSRSSAATIPLNIETQTNKLGNNSVIANFSATFGAT
                     IGQNGCAGIYPAMLAVMVAPTVGMDPFSLNYILTLILVVAISSFGIAGVGGGATFAAI
                     VVLSTLNLPIELVGLLISVEPIIDMARTALNVNGAMVAGTLTNKWVKAE"
     gene            complement(2289..3041)
                     /locus_tag="PXD81_001151"
     CDS             complement(2289..3041)
                     /locus_tag="PXD81_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-ribbon-containing protein"
                     /protein_id="HDL5537185.1"
                     /translation="MYQAIVHFSYHNFEQDPVTAISQILNQWLYNGQVIGREMPITFH
                     QNEFQARVCVPTQESLLPQYNSDEVNEALQQAVEIGVIFASFELVGRDYQGEESSHNR
                     SPAFQILYTTHLDTCSPLYDGEQFAPIPLYSLQDQSLSEAILEWQQNWQACDLLQMNG
                     NLLECHALVQISDETSDLAMIGRDLCQQIEQKTHIPTFYYLYRLGRDESEYQRKCPCC
                     GGEWKLTEPLHDIFHFRCEPCRLISNLSWELL"
     gene            complement(3041..3946)
                     /locus_tag="PXD81_001152"
     CDS             complement(3041..3946)
                     /locus_tag="PXD81_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HDL5537186.1"
                     /translation="MNEKYSKLVKNAANLAIIVAILLIVIKAFAWWKTGSISILAAMT
                     DSVVDLFASLTNILVLRFALQPADENHAFGHGKAESLAALAQSAFISGSAVFLLLQGF
                     HKLTNPELIAQSSIGVIVSIVSIVITALLVFYQRYVVTLTKSPAIEADSLHYQTDLLM
                     NAAILVAMLLNSAGFVYADAVFAIGIALYIAFNAVKMFWCAVQTLLDQALPDEEIERI
                     KEIASQHPNILGIHDILTRRAGAVRFIQLHLELDDHLTLLVAHEITDSLEQKLLAAFP
                     MSHVIIHQEPTTVVKQEFQDMAENI"
     gene            complement(3955..4518)
                     /locus_tag="PXD81_001153"
     CDS             complement(3955..4518)
                     /locus_tag="PXD81_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATPase"
                     /protein_id="HDL5537187.1"
                     /translation="MSAIKKGITLVALSLAGCVSQSPQFAVPDAIHFGQDTFVKVTHS
                     QIDEMQHLLYLPEKSEKNPENWQKGILFFLDKNSKNQTLEQRAAFRQASFAKKPELEA
                     KVEIQQNELHSSVIYPPIERFNNVMLEVTRGRNLACGYGQIQFADKREVGKTVAKKSP
                     NLTAYSQEIARLSLELNQLGWQVQCSK"
     gene            complement(4518..5294)
                     /gene="yaaA"
                     /locus_tag="PXD81_001154"
     CDS             complement(4518..5294)
                     /gene="yaaA"
                     /locus_tag="PXD81_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005622827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HDL5537188.1"
                     /translation="MLAIISPAKTLDFKTNIDGFAFSQPNLTACSQELIDICKQLSPA
                     ELGSLMSISDKLAALNVARFAEWQLEHNEQNAKAALFAFKGDVYTGLEAETLSKAQVE
                     YAQQHLRMLSGLYGLLKPLDLMQPYRLEMGTKLANPKGKDLYAFWGNIITENLQQAID
                     EQGDDILVNLASDEYYGAVKPQNLNATIIKPVFLDEKNGKYKVISFYAKKARGMMVRF
                     ILDTQPTSIEQLKQFNYGGYWFDADSSSESELVFKREEQA"
     gene            complement(5483..7203)
                     /gene="ltrA"
                     /locus_tag="PXD81_001155"
                     /pseudo
     CDS             complement(5483..7203)
                     /gene="ltrA"
                     /locus_tag="PXD81_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012341372.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
     gene            complement(8885..9280)
                     /gene="rpsI"
                     /locus_tag="PXD81_001156"
     CDS             complement(8885..9280)
                     /gene="rpsI"
                     /locus_tag="PXD81_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015432457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HDL5537189.1"
                     /translation="MTAANQNYGTGRRKSSSARVFIKPGSGNITINQRSLEVYFGRET
                     ARMIVRQPLELVELTDKLDLYITVKGGGISGQAGAIRHGITRALMEYDETLRPALRAA
                     GFVTRDARRVERKKVGLHKARRRPQYSKR"
     gene            complement(9297..9725)
                     /gene="rplM"
                     /locus_tag="PXD81_001157"
     CDS             complement(9297..9725)
                     /gene="rplM"
                     /locus_tag="PXD81_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HDL5537190.1"
                     /translation="MKTFVAKPETVQRDWYVVDATGKTLGRLATELARRLRGKHKPEY
                     TPHVDTGDYIIVINADKVAVTGKKQTDKIYYWHTGYVGGIKDATFKEMIARRPEAVIE
                     IAVKGMLPKGPLGREMFRKLKVYAGSEHNHQAQQPQVLDI"
     gene            10024..10419
                     /locus_tag="PXD81_001158"
     CDS             10024..10419
                     /locus_tag="PXD81_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012262875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcB family protein"
                     /protein_id="HDL5537191.1"
                     /translation="MEQWTTDVWAAIMAAFIVGAVIGCTLVRVFNSSVRKQHELEKEL
                     KQTKTQVDEQKQKLEQHFEQSAQLLSSLASDYKKLYTHLAQSSEKLLPEASKNITFFQ
                     QTKLPIPAEKTEEDQPRDYSEGSSGLLKS"
     gene            complement(10580..12151)
                     /gene="guaA"
                     /locus_tag="PXD81_001159"
     CDS             complement(10580..12151)
                     /gene="guaA"
                     /locus_tag="PXD81_001159"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012510808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HDL5537192.1"
                     /translation="MTNIHHHKILILDFGSQYTQLIARRVREIGVYCELWAWDVTEEQ
                     IREFNPTGIILSGGPESTTEENSPRAPEYVFNAGLPVLGICYGMQTMAMQLGGLTETS
                     DHREFGYASVALKSADQLFANLNDDLTACEPKLDVWMSHGDKVTKLPPHFTITGVTPT
                     CPIAAMSDESRRFYGVQFHPEVTHTKQGLALLKNFVVGICGCETNWTAENIIEDAVKR
                     IREQVGDDEVILGLSGGVDSSVTALLLHRAIGKNLHCVFVDNGLLRLNEGDQVMEMFG
                     NKFGLNIVRVNAEDRFLEALKGLDEPEAKRKTIGKVFVDVFDDESKKLSSVKWLAQGT
                     IYPDVIESAASKTGKAHVIKSHHNVGGLPDYMKLGLVEPLRELFKDEVRKIGLALGLP
                     AEMLNRHPFPGPGLGVRVLGEIKKEYCDLLRKADAIFIEELHKAGWYYKVSQAFTVFL
                     PVKSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHLPYDLLGKISNRIINEVNGISRV
                     VYDVSGKPPATIEWE"
BASE COUNT         3496 a         2759 c         2326 g         3689 t
ORIGIN      
        1 atgattgatt tttaaggtaa agcaatttta aacaagcggt cattttttcc taattttttg
       61 caaattaaca ggcgtgaaat tcgcattctg ctctaaatca agttatcatt aatctactta
      121 tttcacttat aaaaggaaaa acgatgttta atattagcaa acagcaagtt ttagacgctt
      181 ttcacttccg ttcggcctgt cgcagttacg atccaaacaa aaaaatcaac aaagaagata
      241 tggattatat tttggaactc gggcgtctct caccaagctc tgtcggttct gaaccgtgga
      301 aatttttagt gctacaaaac ccggaaaccc gtcagaaaat cgccccggtt tcgtggggaa
      361 ttaaacaccc aatggaagaa atgagccatt tagtggtgat tttaaccaaa aagaatgtcc
      421 gttatgattc tgtctatatg cgtgatatgt tagccaaacg aggcttaacc tatgagcaga
      481 ttgaagcctc gctggtacgc tataagtcgt tccaagaaga ggatatgaaa ttgcttagtg
      541 aacgtgaatt attcgattgg agtaccaagc aaacctatat tgcattaggc aatatgatga
      601 ccggtgcggc tctgattggt attgactctt gcccgattga aggttttcat tacgataccg
      661 taaaccaaat tttatcggac gaaggcttat ttgacctaaa cgaatatggt gtgtcttgta
      721 tgataacttt tggctatcgc aacaaagaaa ttaagaaaaa atcccgcaaa cctaccgaag
      781 aagtgattgc gtggattgaa taatcacctt aacttataaa aacgggcgaa ttaatcgccc
      841 atttttatat ctttagtttt gactactcgg ctttcaccca tttattggtt aatgtgcccg
      901 ccaccattgc accattcacg tttaaggcgg tgcgagccat atcaataatc ggctcaactg
      961 aaatgagcaa gcctactaac tcaatcggca gattcaaggt tgatagcacc acaatcgccg
     1021 caaaagttgc accaccacct acaccggcaa tgccaaatga ggaaattgcc accaccaaaa
     1081 tcaaggttaa aatatagttt aagctaaacg gatccattcc cactgttgga gcgaccatca
     1141 ccgccaacat tgccggataa attcccgcac agccattttg cccgatggtt gccccaaagg
     1201 ttgcagaaaa gttggcaatc acactgttat tacctaattt attggtttgc gtctcaatat
     1261 tcaacggaat ggttgccgca ctggaacgag acgtaaaagc aaaacttaat gtcggcaaca
     1321 cttttttgta gtaatccaac gggttgattt tagccacaaa gagtaaaata ccgtgcacaa
     1381 taaacatcaa accaatcgca atgtatgatg ccacaataaa gctacctaaa tttacaatat
     1441 cgctccattg agaagttgcc gccattttga tcatcaacgc aaatacgcca taaggggtta
     1501 ggcgaatcac aaaacgtact aaacgcataa ccagtttatt taaggtctct acaccttgtg
     1561 cgatacgttc gcccaacgct ttatcttctt tgcctaagct cagtgcagca atgcctagca
     1621 gagccgagaa aataaccgtg ttgatgatcg acgttgggtt tgcaccggtt aattctaaaa
     1681 acggattttt cggaatgaaa gaaagcaaca ttgaaggcac agataagttg ctcacctgct
     1741 ccacacgccc taccactttc tcttgagcgg caagctcacg ggagccggcg actaaaccct
     1801 cggcagataa atcaaacaga tacactaaag caataccaat cgctgcggca atcgcggtgg
     1861 tcactaataa cactgatagc acactaaaac taattttgcc taaagatcct gcctctttta
     1921 agcgggtaat tgccgataaa atagagacaa acaccaatgg cattacgatc atttgtagca
     1981 agcgaacata accgttacca attaagttaa tccaatcaag agttgcatca acagtagctt
     2041 tttcgtaaaa agtttgcaaa aaagcaccgc ttgcaagccc taaaaataac ccaataaaca
     2101 ctgtttgacc gagtttttgc gtagatttat aaattttagc caataaaaat aatattgccg
     2161 caaagacaat taagttgaaa ataagcacag aacctccaaa gtaaaaccgc ttattataaa
     2221 aatgccgcaa gggtattacg cttgcggcat tcattcaaga tgatttatta ctttggtata
     2281 actaaaagtt ataataattc ccagcttaga ttagagatca aacggcaagg ctcacatcta
     2341 aaatggaaaa tatcgtgcaa cggctcagtt aatttccatt ctccgccaca acaagggcat
     2401 ttgcgttgat attcactttc atcacgccct aaacggtaga ggtaataaaa ggtgggaatg
     2461 tgagttttct gctcaatttg ctgacataga tctcgaccga tcattgcaag atcgcttgtt
     2521 tcatcactaa tttgaacaag tgcgtggcac tccagcagat tgccgttcat ttgtagcaga
     2581 tcgcacgctt gccaattttg ctgccattcc aatatcgctt cgctaagaga ctgatcctgt
     2641 aaagaataaa gcggaattgg ggcaaactgc tctccatcat aaagtggcga gcaggtatct
     2701 aaatgggtgg tatataatat ttgaaacgct ggcgagcggt tatgactgct ttcttcgccc
     2761 tgataatctc gcccgacaag ctcaaaactt gcaaaaatta cgccgatttc gaccgcttgt
     2821 tgcaacgctt catttacttc atcactatta tattgtggca aaaggctttc ttgtgtaggc
     2881 acacaaaccc gagcttgaaa ctcgttttga tgaaaagtaa tcggcatttc tctgccaatg
     2941 acttgcccgt tatataacca ttgatttaag atttggctaa ttgccgtcac cggatcttgc
     3001 tcaaaattat gatagctgaa atgcactatc gcctgataca ttaaatattc tccgccatat
     3061 cttgaaattc ttgtttgacc accgtagtcg gctcttgatg aataatcacg tgagacatcg
     3121 gaaatgccgc cagcaatttc tgctcaaggc tatcagttat ttcgtgagct actagcagcg
     3181 ttaagtgatc gtctaattcc aaatgtaatt ggataaaacg cactgcacct gcacggcggg
     3241 ttagaatatc gtgaatccct aaaatgttcg gatgttgcga agcaatctct ttaatccgct
     3301 caatttcttc atcgggcaaa gcttggtcta gcaaggtttg caccgcacac caaaacattt
     3361 ttactgcatt gaaagcaatg tagagggcaa tacctattgc aaaaactgca tcggcgtaca
     3421 caaaaccggc actattaagc aacattgcca ctaaaattgc cgcattcatt aataaatcag
     3481 tttggtaatg taacgaatcc gcttcaatcg ccggactttt cgtaagcgtg acaacataac
     3541 gctgataaaa gactaaaagt gcagtaatca caattgatac aatactgaca attaccccaa
     3601 tactcgattg agcaattaat tccggattgg tgagtttatg gaaaccttgt agcagtagga
     3661 aaaccgccga gccggaaata aacgcacttt gggcaagtgc cgccaaagac tccgctttgc
     3721 cgtgcccaaa cgcgtgattc tcatctgccg gttgtagtgc aaaacgcagc actaaaatat
     3781 tggttagcga agcaaacaga tccactaccg aatccgtcat tgctgccaag attgaaatcg
     3841 aaccggtttt ccaccatgca aaggctttaa tcacaatcag taagatcgca acaataatcg
     3901 ctaaattcgc tgcatttttg actaatttgc tgtatttttc gttcatttac tacattattt
     3961 actgcattgg acttgccaac ccagttggtt taattcaaga gaaagtctag caatttcttg
     4021 gctgtaagcg gtcaaattcg gcgatttttt tgcaactgtc ttgccaactt cccgcttatc
     4081 tgcaaattga atctgcccgt aaccacaagc taaattccgc ccacgggtta cttctaacat
     4141 cacattgtta aaacgttcta taggtggata aatcacactg ctgtgtaatt cattttgctg
     4201 aatttccact ttcgcctcaa gctccggctt tttcgcaaaa cttgcttggc ggaatgcagc
     4261 tcgttgctct aaggtttgat ttttactgtt tttatctaag aaaaataaaa tgcctttttg
     4321 ccaattttcc ggatttttct cactcttttc tggcaaataa agtaaatgtt gcatttcatc
     4381 aatttggctg tgggtgactt ttacaaaagt atcctgccca aagtgaatag catcaggcac
     4441 tgcaaattgt gggctttggc tgacacaacc tgccaatgat aacgcaacca aagttattcc
     4501 ctttttgata gccgacatta tgcctgctcc tcacgtttaa agactagctc gctttcacta
     4561 gaactgtctg catcaaacca atagcccccg tagttaaact gtttgagctg ttcgatgcta
     4621 gtcggttggg tgtctaatat aaagcgaacc atcattccac gggctttttt ggcatagaag
     4681 ctgatcactt tatatttacc gtttttctca tctaaaaaca ccggcttgat aatagtcgca
     4741 tttagatttt gtggcttaac cgcaccataa tattcatcag aggcaagatt aactaaaata
     4801 tcatcgcctt gttcatcaat cgcttgctgc aaattttcag taataatatt gccccagaaa
     4861 gcataaagat ctttgccttt cggattggct aacttagtgc ccatttctaa acgataaggc
     4921 tgcattaaat ctaacggctt taataaacca tacaagcctg aaagcatacg caaatgttgt
     4981 tgagcatatt caacttgtgc tttgcttaac gtttctgcct ctagtccggt ataaacatca
     5041 cctttaaagg caaagagggc ggctttggca ttttgttcgt tatgctcaag ctgccactcg
     5101 gcaaagcgag ccacatttaa agcggctaat ttatcgctaa ttgacatcaa actacctaat
     5161 tcagcaggtg aaagctgttt acaaatatca attaattctt ggctacaagc ggtcaaattt
     5221 ggttgagaaa atgcaaaacc gtcaatattg gttttaaaat caagggtttt ggcaggggaa
     5281 ataatggcta acataaatgc ctctatcaaa tagttaatca gggcggttat tctaccacag
     5341 tcataaagac aaaaaaggca ggaaaccctg ccttcttgaa aacgaaaaga aaagtcgggt
     5401 agcgaaagta cattactgca cctccgcccc ctaagaactg tacaagcaaa tttctctgca
     5461 tacagctcaa gcctttcatc agctatatag ccggcttcac gacctccaag ttcaaagtat
     5521 gaatctttat gtgacaagtc ggatgtacca aacaacgatt gcttaatgca tcgcttcctc
     5581 ctaacatttt gtacacgata tgatgatcgt gccagcctgt ttctttggtt attggcaacc
     5641 cacataatgc acattttcca cgctggtctt tatacagtgc ctgccattgt cgtctgtgtg
     5701 attgttcttc atacagacgt tcttggctca gtttttcgcc gtacaactcc cattcaggca
     5761 aaaacggatt atattcagac ttaattttaa cgtgtcgttt gattttaaca tagccacaat
     5821 agaataagtc tatcggctta gtttctcctt tttcattggt ataaactgtg ccaaataccc
     5881 aatttcgagt cgctgtggta caaaagtatt tctcttttat ccacataata cctttattgt
     5941 gatgacggcg ttcacaccat ttccaaagta ctttccaaac taatgcgtcc attcgtccga
     6001 atgccttact tgcaacttgg cttcgatgat aatttgccca acctcgtaac attgggttta
     6061 gcaccattat aagatcttcc tgtttagcca ttttcattta gaaattacct cttttacctt
     6121 gttgtagaac gctttcacat tcttttgact tggtctgttc agtatttttc ctttgaaacg
     6181 tctcacattc caacctagaa aatcaaaacc ttcttcaata tgtaccactt tcgtcttttc
     6241 tgttgataag gataaacctc gttcattcag aaagttctgg ataagtggga taacttgatt
     6301 ttcaagtaac tctttcgttt tacctgatat gataaagtca tctgcataac gtactaagta
     6361 agttttattt tctcgaattg tccgactgcc ttttcctcca aaatgagttt ctaacagttt
     6421 ttccaatcca tctaatgcca tatttgcaag cgttggcgag ataatcccac cctgtggtgt
     6481 accttcctct gttcgtttca accgtccaaa ttccactaca cctgatttta gccatttccg
     6541 caatatccgt ttattcattg gaatatgctt taatagccag tcgtggctta tgtggtcaaa
     6601 acaacctgca atatccgcat caagtaccca ctcaacaggt tgattagttt tcttgccttt
     6661 atgcgaataa atttagtgta tatgagtaat agcatctacc gttgaacggt ttaatctaaa
     6721 tccataagaa cctttatccg cagttgtttc tgccacaggt tgtattgcca ataaataaag
     6781 ggcttgcatt gctctatctc gcattgttgg aatacccaac ggacgtttct ttccattcgg
     6841 tttggggata tacactcgtc ttaatggact aggtttatac cctttttccg tcaatgatag
     6901 tgcggcttgc cattttgtct ctggggtctc ccaaagctct ttatcgacac cacttgttcg
     6961 cttaccttga ttttcagtca ctcttcttac tgcaactaat ttagcataat gtgagtgggt
     7021 tagcattcgt tgtaagtttt taaccttacg ccaattgcct tccagtgttg ccttcgcaat
     7081 ccgaatttgc attcctttca catacgtttc agctttatgc caatcgacat aatgccattt
     7141 ggagatgtgc gaggtcgctg gattgcaagc taacttttca gtataatgct tacgctcttt
     7201 catacctacc tctgatatta caagataagt cgaaagtctc agcaagtggg tagatccttc
     7261 aagtaaacaa atccctattg agataaacta tccttagttt attccctttg gttacaagag
     7321 gatcttaccg ttttaccgat aatttcaacc gctcttgtaa ccgattgggt taaagagtcg
     7381 agcgctaggt tatgatgagc ctaacgctca tttctttaat ttgtttttgt acctattcaa
     7441 cattgtattc cttgataaca ttcaatttct cttctgttat ctacaatctc tttcggtgtt
     7501 acccttaaag gaaacgctat tcatcttacg atgaaagacc agttagaagt ctgcaccctt
     7561 tcgggttagg ctatgtttca caccctatct atgccattac agcatagcat tcgctttttc
     7621 taacctccct tacctgcatt cccaacagta tcgcttacgc tttacttgcc ttttcctctt
     7681 ttaccttcgc catctatttc aaacggtttg agtattgtgg gcaaggcaga aatacaggct
     7741 taccaagttc cattactatc acacgagtag tgaagataca gtctctcccc cgacagtgct
     7801 ttgatggcgt atcctcacgt taaagaagaa taaccgactg cctacctttt ggtcagagcc
     7861 tatcaatagc tttggctctc tctcgataac gaggtttatc aactgttcac ttacgtttat
     7921 ctttctaccc agtctagccc cttgaaggtg tgctattcac tttctcccaa tgtcctctca
     7981 cgatttcatt gactgatttt catcaggggt tcattgtcag gaagctccgc accacaccgt
     8041 taccggtgtc gcactatccc tagactactg ttagccatat aacaggttac cttatcattc
     8101 cctccgtact actcatatct tcggattgtt tgaccgtaac aatgacagta atagcttacg
     8161 catacttact aagtcagtat cctatactgc ataaataagt ccgaaccttt acaaaataaa
     8221 ttcacttatg cgactaaagt cgctttgtct atatccaaat tttcccgcaa atagcctata
     8281 acctgtccaa taggcgaagg tagctaaccc tagctaatgt gagtattaga ggtgatttta
     8341 acgagaattt aaggtataat tatccctagc tcataaaaac gaataaacaa gagtttatcc
     8401 ttagtgtttt tattcactat tgaggagcat tctacgatta ccgtagattg cgagtgtgaa
     8461 aagtataagt aaagatggca ctccttccct aagatttacc aagtcttagt tctgatgtct
     8521 tttctgagtg ctgaggcgat ctattggact ttatttatag taggtgttgt aaattttctc
     8581 tgctctgggg tatgggtaac tccggtctgc ggatcttgct ttcttactct atcttggtat
     8641 taagaaaaaa accaagtaca tcaaagtgaa gatcatacct tacagccttt cgagttggtt
     8701 catttacttt cccaacttag aacaagagtg cttgggcaaa gccccttttc tcttgcctgt
     8761 ctttccaagc actccgaaga gtgcttggaa agtcggaagc aggtgataag aataccaccc
     8821 acttccagca ataactcctt gatatataag gagttattgc cataagtacg acaacttgtc
     8881 gcacttaacg ttttgagtat tgtggacgac gacgtgcttt gtgtaaaccc acttttttac
     8941 gttcaacgcg acgtgcgtca cgagtaacga agcctgctgc acgaagtgct gggcgtagcg
     9001 tttcatcgta ttccattaat gcacgagtga taccgtgacg gattgcaccc gcttgaccag
     9061 aaataccacc acctttaaca gtgatgtaaa ggtctaattt atcagttaat tcaaccaatt
     9121 ctaacggctg acgtacgatc atacgagctg tttcacgacc gaagtaaact tcaagtgaac
     9181 gttggttgat agtaatgtta cctgaacccg gtttgataaa tacacgggct gaagagcttt
     9241 tgcggcgacc tgtgccgtag ttttgatttg ctgctgtcat ttttatgctc cgtgaattag
     9301 atatctaaaa cttgtggttg ttgtgcttgg tgattgtgtt ctgaacctgc gtaaactttt
     9361 aatttacgga acatttcacg accaagtgga ccttttggta acataccttt cactgcgatt
     9421 tcaatcacag cttcaggacg acgagcaatc atttctttga aggttgcatc tttgatgcca
     9481 cctacatagc cagtgtgcca gtagtagatt ttgtcagttt gttttttacc tgttactgct
     9541 actttgtctg cattgatcac gatgatgtaa tcaccggtat caacgtgtgg agtgtactca
     9601 ggtttatgtt taccacgaag acggcgtgcc aattcagtcg ctaaacgacc taatgtttta
     9661 cccgtcgcat ctactacata ccagtcacgt tgtactgttt ctggttttgc tacaaaagtt
     9721 ttcattaatt aaataccaaa tttaaagttt aaaacccaat acctcattga ggtatttcct
     9781 attattttac ggtgaacccg accccttcga gtctgattca gaccaaactt gtgtggcggg
     9841 gaaaaccaca caaaaacagg gtggcgaaat tatacagaga tttcactcaa aaatctaaca
     9901 ttttttgctg ataatctttc aaaattgaac ctatatcaca ttttaatcat ttggagcttt
     9961 tcaaataaaa actgaaatgt taaaaatagc agtaatcaat cactaaaaca taaggagttt
    10021 atgatggaac aatggacaac agatgtttgg gcagccatta tggcagcatt tattgtaggt
    10081 gctgtaatag gttgcactct agttagagta tttaacagta gtgtcagaaa acagcatgaa
    10141 ctggaaaaag agttgaagca aaccaaaact caagtggatg agcaaaaaca aaaacttgaa
    10201 caacactttg agcaatcagc ccaattactt tcaagtctag cttctgatta taaaaagctt
    10261 tatacccatt tagcacaaag ctctgaaaag ctattaccag aagcatcaaa aaatattacc
    10321 tttttccaac aaaccaaatt acctattccg gcagaaaaaa cggaagaaga tcaacctaga
    10381 gattattccg aaggttcatc tggcttactg aaatcataaa actaaaaatt gaatatttag
    10441 ttttactaaa aggcacttag tttaaggatt gattccgttg gaatcaatcc tttattctaa
    10501 accgctacat taagcagtta acaacatttt tcagctttag atactgaact taggttataa
    10561 gaagcctatt ttgtaagagt tattcccact caatggttgc cggtggtttt ccgctcacgt
    10621 catacaccac acgggaaata ccgttcactt cgttaataat gcggttggag attttgccta
    10681 atagatcata cggcaagtgc gcccagtgag cggtcataaa gtcgatggtt tccaccgcac
    10741 gcaggctgac gacccagtca tatttgcgtc catcgcccat tacgcccacc gatttcaccg
    10801 gcaagaatac cgtgaaagct tggctgactt tgtagtacca gccggctttg tgcagttctt
    10861 cgatgaagat cgcatcggct ttacgcagta aatcgcagta ctcttttttg atttcgccca
    10921 gcacacgcac gcctaagccc gggccgggga acgggtggcg gttgagcatt tcagccggta
    10981 agccgagtgc caagccgatt ttgcgcactt cgtctttaaa caattcacgc aacggttcga
    11041 ctaagccgag cttcatataa tccggcaagc cgccaacgtt atggtgtgat ttaatcacgt
    11101 gggctttgcc ggttttgctg gcggcggatt caatcacatc agggtagatc gtgccttgtg
    11161 caagccattt gaccgagcta agttttttgc tttcgtcatc aaacacgtcc acaaacactt
    11221 taccaatggt tttgcgttta gcttccggtt cgtctaagcc ttttaacgct tctaagaaac
    11281 ggtcttcggc attcactcgc acaatgttca aaccgaattt gttgccgaac atttccatga
    11341 cttgatcgcc ttcgttcaag cggagcaagc cgttatccac aaatacgcag tgcaggtttt
    11401 taccgatagc acgatgtaat agcaaggcgg tgacggagga atccaccccg cccgacaaac
    11461 ctaaaatcac ctcgtcatcg cccacttgct cacgaatgcg tttcaccgca tcttcaatga
    11521 tgttttctgc cgtccagttc gtttcacagc cgcaaatgcc gaccacgaag tttttcagca
    11581 acgccaagcc ttgtttggtg tgggtcactt cggggtggaa ctgcacgcca tagaaacggc
    11641 ggctttcgtc cgacatcgcg gcaatcgggc aggttggggt aacgcctgtg atggtaaagt
    11701 gcggtggtaa tttggtgact ttgtcgccgt gactcatcca cacgtccaat tttggctcgc
    11761 aagcggtcaa atcatcgttt aaatttgcaa aaagttgatc ggcagatttg agagccaccg
    11821 aggcatagcc aaattcacgg tggtcggacg tttcggttaa accgcccaac tgcatcgcca
    11881 ttgtctgcat tccgtagcaa atgcccagca ctggcagacc agcgttaaac acatactccg
    11941 gcgctcgtgg gctgttttcc tcagtggtgc tttcaggacc tcctgaaaga ataatccccg
    12001 ttgggttaaa ttcacggatc tgctcttccg ttacgtccca agcccaaagc tcgcagtaaa
    12061 ccccaatctc ccgcacacgg cgtgcgataa gctgagtata ttgtgaaccg aagtctaaaa
    12121 ttaaaatttt gtggtggtgg atgtttgtca ttgtaatttc ctttaaatta tggcgttgtg
    12181 gtcatgttaa taaaagtgcc gcctacccca tcgtttctat atgtaataat aaaagattgc
    12241 gttttattac aacctgttgc agtccaaagt
//
LOCUS       DANLYI010000057        12145 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.057, whole genome shotgun sequence.
ACCESSION   DANLYI010000057 DANLYI010000000
VERSION     DANLYI010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 12145)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12145)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12145
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.057"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            89..1087
                     /gene="yiaK"
                     /locus_tag="PXD81_001160"
     CDS             89..1087
                     /gene="yiaK"
                     /locus_tag="PXD81_001160"
                     /EC_number="1.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005757429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydro-L-gulonate 2-dehydrogenase"
                     /protein_id="HDL5537193.1"
                     /translation="MRVSYEELKAQFKRVLLARNVREEIAEECATVFADTTQAGAYSH
                     GVNRFPRFIQQLENGDIVPEAVPTKVLSLGAIEQWDAHQAIGNLTAKKMMDRAMELAS
                     ENGIGVVALRNANHWMRGGSYGWQAAEKGYIGICWTNALAVMPPWGAKECRIGTNPLI
                     IAVPTNPITMVDMSCSMYSYGMLEVHRLAGRQTFVDAGFDDDGNPTRDPATVEKNLRL
                     MPMGFWKGSGLSIVLDMIATLLSNGESTAAVTEDKDDEYCVSQVYIAIEVDRLIDGKT
                     KDEKLNRIMEYVKTAERADPDVAVRLPGHEFTQILADNKANGIPVDDTVWAKLQAL"
     gene            1212..1577
                     /locus_tag="PXD81_001161"
     CDS             1212..1577
                     /locus_tag="PXD81_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HDL5537194.1"
                     /translation="MFFGHISQVKANRYPPAIQIALNYLKNTDFDAMEAGVYELKGRQ
                     IYVQVLDLNTKSKHEFQPEVHRNYLDVQYLHRGKEIMAAAVDTGTNPIAMEYNPERDI
                     QYYQSVANENEFRCVEGNF"
     gene            1641..2120
                     /locus_tag="PXD81_001162"
     CDS             1641..2120
                     /locus_tag="PXD81_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HDL5537195.1"
                     /translation="MKALAQLVGKLLEILVVVILSAMSILVFVNVVLRYGFNSSISVT
                     EEVSRYMFVWLAFLGAVLAFSENQHVKVTMLTDKLSPTARNILSVVTDLAMLFCCYLI
                     VDGSWIQFNLNLNNFAPISGLPQGITFLASTVAGVLIGLLIIARIVATVGVIAKGEK"
     gene            2121..3398
                     /locus_tag="PXD81_001163"
     CDS             2121..3398
                     /locus_tag="PXD81_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017806296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HDL5537196.1"
                     /translation="MTVVIFLSVLLGSILLGIPVAFALLVCGVALMLHLDLFDAQILA
                     QQIVSGADSFSLMAIPFFILAGEIMNEGGLSKRIIDLPMKLVGHKRGGLGFVAILAAM
                     IMASLSGSAVADTAAVVAMLLPMMKTTGYPIDRSAGLIGTAGIIAPIIPPSIPFIVFG
                     VASGVSITKLFLAGIFPGIMMGICLAALWWWQAKRLDLMTFSKATKEELCISFKNSIW
                     ALLLPVIIIGGFRSGMFTPTEAGAVATFYALIVSLFVYREMKFKDLYKVVLAAAKTTA
                     VVMFLVAAANVTGWLITVAELPMMLTELLEPLIESPTTLLLVIMLAVFIIGMVMDLTP
                     TVLILTPVLMPLVEEAGIDPVYFGVLFILNTSIGLITPPVGNVLNVITGVSKLPFDQA
                     AKGVMPYLVMMICLLLLFVFFPSLVLVPLSWMQ"
     gene            3501..4487
                     /locus_tag="PXD81_001164"
     CDS             3501..4487
                     /locus_tag="PXD81_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HDL5537197.1"
                     /translation="MKLFNLKTLFALVAGVAVMSGSAMAETSLRFGYEAPRSDTQHIA
                     AKKFDELLAAKTKGEIKLKLFPDSTLGNAQTMISGVRGGTIDIEMSGSPNFTGLEPKL
                     NVIDIPFIFKDREHVYKVLDGEVGQGLLKDLEAQGLKGLAFWDVGFRAFSNSKHPVTK
                     PEDIKGLKVRTNQNPMYIQAFTLLGGNPVPMPLSELYTALETRAVDAQEHPIGIFWSA
                     KLFEVQKHLSLTNHGYTPLIVVMNKAKFDGLAPELQTALVEAAKEAGQFQRDLNVKNE
                     KEIIEKLRKQGIEVIEQVDNAPFKAAIEAEVRKAFVEKHGDELLKKVDALAQ"
     gene            4561..5544
                     /locus_tag="PXD81_001165"
     CDS             4561..5544
                     /locus_tag="PXD81_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HDL5537198.1"
                     /translation="MKLFNLKTLAAFVAGFALFSTAQAETSFRFAYEAPRSDTQHIAA
                     KKFNDLLQEKSQKGLKLSLFPDSTLGNAQTAISGVRGGTIDIAMSSSSNFTGLEPKLN
                     VIDIPFIFKDRDHAYKALDGEIGQGLLTALEAHGLKGLAFWEVGFRAFSNSKHPITKP
                     EDIKGLKIRTNQNPMYIQAFSILGANPVPMPLSKLYTALETKAVDAQEHPIGIFWSAK
                     LYEVQKYFSFTNHGYTPLIVAINKAKFDALPAEQQTALVEAAKEAGKYQRQLNLENEQ
                     KIIDSLKKAGIEFVDNLDTTPFKDAVSAETRKAFIEKNGDGLVKAIDALAK"
     gene            5675..7159
                     /locus_tag="PXD81_001166"
     CDS             5675..7159
                     /locus_tag="PXD81_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HDL5537199.1"
                     /translation="MQHIIKRFGSHTAVNDVSLDIHSGEIVALLGENGAGKSTLIKIL
                     AGIYDRDEGDILFHNQKIQSAHAIEKGNKKPIAFIHQDLGLIEWMTIAENMAFVMGFP
                     RKFGLIDWAKVREQSQKALDFVGIKLSADTRVFDLSRTEKALLAIARAIAVNAEILVL
                     DEPTASLPASDVEHLFTVLNRLRSQGVGMIYVTHRLDEVIAISDRILVMRDGFPVAEG
                     QTKLYDVKGLVKAIVGEESRGRQRVDLPTDTPEILSLQNVVVGDTGPVNFRLHKGEMI
                     ALAGLRGAGQEEIGRLLFGERTLDKGEIQLDKRSYSAKDPQDAIAKGVALVAGDRTRE
                     SVFMSMTTSENLFINPVLSGHSPLKRYSRREEWGESWYKFQLFDIRPKNLFIDASALS
                     GGNQQKIVLARWMHLNTPVLILEDPTAGVDVGARAEIYDLLNKALAEGVAIIVISTDF
                     EEIAHLCNRALVFNRGEIAGELLNEEVTFANLLALASDSQSKKQ"
     gene            7354..8400
                     /locus_tag="PXD81_001167"
     CDS             7354..8400
                     /locus_tag="PXD81_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL5537200.1"
                     /translation="MAQTNIKSTALEQPVSIARDGFMAWFSQMSTRYGLLWLCVLLVI
                     IFSLTTESFASMLTLNAILESKSKIALLALAATTTMIVGKIDLNVGFGIVLWHILVIT
                     LQVQYGFSWYMAILIVLVIAALYGLLNGILVALADIDSFVATLGSGTVLYAVALWHSG
                     GRQIVGDLPDEFIALNSTEIFGIPISAFYVLAVAIVMWLVTEHTPTGRCMYAVGGNPT
                     AARLNGINIKKYTIVPFIVSSVITAFTGVLISAQQGVGQASVGMDYLLPALVGAFLGS
                     TTIRPGRINVWGTVVGIAILAIGISGIQQFGGAFWVEPLFNGATLLLSITIAGYAQRK
                     RLLNQKAVQKKSTN"
     gene            8413..9522
                     /locus_tag="PXD81_001168"
     CDS             8413..9522
                     /locus_tag="PXD81_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005757420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HDL5537201.1"
                     /translation="MKKAFRTTLTAIATLTAIAFGNNAFANDDPFVQEAKQLVAAATA
                     KQEKWDGPTTGPQLQKEKTVIFIASDMKNGGVLGVIDGMKEAVGVTGWKLDVLDGAGT
                     VNNQLAALNQAIARKPDAIVIGGWNPNVAKIPLQKATKNGIKLAAWHATPEPGAIDKY
                     GIFYNVTSDSDEIARLSAMLAVAESNGKAKAIILTDSLYEIALRKANVMKEVIEKCKE
                     CKVLEFIDTPLADTSSRMPNLTFSLLQKYGDDLEYALAINDLYFDFMAPSLRTAGKDK
                     PFSISAGDGSVTAYQRIRNGDKQFATVPEPLNLHGWQLVDELNRAFANEKPSGYITPA
                     HLVTKENVAFDGGENNLYAPQNGYKGAYKAIWGVK"
     gene            9627..10517
                     /locus_tag="PXD81_001169"
     CDS             9627..10517
                     /locus_tag="PXD81_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HDL5537202.1"
                     /translation="MSNKFIPKISNPRFNELVGNTFELEELFDKAIWAEGPAWSKPQQ
                     ALYFSDVKGCTMYRWTEQDGTQVFRQPSYFSNGNAFDANDNLITCEHGNRGISLTTPS
                     GEYRFLVGQLDGKRLNSPNDVVVKSDGIIWFTDPPYGILSDNEGKQSPSEIIGCYIYC
                     YDPKTDKLNIATFNTMRPNGLAFSSDEKQLFVADMSSVEFDEDGLHHLVVFDVDGNKL
                     KNRRDVAEISPGIPDGFRIKQNGVIFCSCEEGIIALLPDGTELGRFVLGKLTSNCSFG
                     DDEQTLFITCSNSVYRLTIK"
     gene            10589..12058
                     /locus_tag="PXD81_001170"
     CDS             10589..12058
                     /locus_tag="PXD81_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005610664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HDL5537203.1"
                     /translation="MDNKMNYTLGIDCGGTFIKAALFDTQGNIQALHRENVSVISEQA
                     GYAERDMSQLWAICAEVVRSTIEKSGVNPADIKAVGISAQGKGAFLLDKQNRPLGRAI
                     LSSDQRSLEIVKQWQAEGIPQQLYPITRQTLWTGHPVSILKWVQQNEPERYAQIGAVL
                     MSHDYLRFCLTGELHCEETNISESNLYNMEKGEYDQQLADLLGMPDVLEKLPPVIEPN
                     KISGYVTENAAKLTGLAVGTPIVGGLFDVVSTARCANLDDETKLNAVLGTWSVVSGIT
                     DHIDASQSLPFVYGRYAEAGKFIVHEASPTSAGNLEWFVNQWKLDYVKINQAVAQLPP
                     AASSVLFVPFLYGSNAGLGMQAGFYGMQAHHTQAHLLQAIYEGVLFSLMSHLNRMFIR
                     FPKADVLRVTGGPVKSKVWMQMLADLTGMRLEIPQMEETGCLGAALMAMQGVGLDISQ
                     VQALNNEMQVFEPNPENYAAYQTKYQRYQKLTEALNTMI"
BASE COUNT         3367 a         2608 c         2921 g         3249 t
ORIGIN      
        1 ttttccttta attgacttta atttgtctta agagtaactt ataaaacgtg attttactat
       61 ttgaaatatt gaggtattca agatgactat tcgagtttcc tatgaagaat taaaagctca
      121 atttaaacgt gttctgttgg ctcgcaatgt gcgtgaagag attgccgaag agtgtgccac
      181 tgtgtttgcc gacaccactc aagcgggtgc ttattcacac ggggtaaacc gtttcccacg
      241 ctttattcag cagttagaga acggtgatat tgtgccggaa gccgtgccga ctaaagtgct
      301 ttcactgggg gcgattgagc agtgggacgc tcaccaagcc atcggtaact taaccgcaaa
      361 aaaaatgatg gacagagcaa tggagcttgc atctgaaaac ggcatcggcg tagtggcgtt
      421 acgtaatgca aaccactgga tgcgtggcgg ttcttacgga tggcaggcgg cagagaaagg
      481 ctatatcggg atctgctgga cgaacgccct tgcggtaatg ccgccttggg gggcgaaaga
      541 gtgccgtatc gggactaacc cgttaatcat tgcggtgccg actaacccaa tcacgatggt
      601 ggatatgtct tgctcaatgt actcatacgg aatgttggaa gtgcatcgcc ttgctggtcg
      661 tcaaaccttt gtcgatgctg gttttgatga tgacggcaac ccaacccgtg atccggcaac
      721 ggtcgagaaa aacctccgtc taatgccgat gggcttctgg aaaggttcgg gcttatcgat
      781 tgtgttagat atgatcgcca ccttgctctc taacggtgaa tccactgcgg cagtcaccga
      841 agataaagac gatgaatatt gcgtgtcgca agtttatatc gctattgaag tggatcgctt
      901 aattgacggc aaaaccaaag acgaaaaact caaccgtatt atggaatatg tgaaaaccgc
      961 agaacgtgcc gaccctgatg tggcagtacg tttaccgggc cacgagttca cccaaattct
     1021 agccgataac aaagccaacg gcattccggt ggatgacacc gtttgggcga aattacaggc
     1081 gttgtaattg caaattgacc ggtagggtgg gcgtaagccc acgcattaga cacaatcata
     1141 agatgacgtg ggcttacgcc caccctacaa gcggtcattt ttccgcagaa ttttacaaaa
     1201 cggaggtatg aatgtttttt ggtcatattt cacaagttaa agcgaatcga tacccgccgg
     1261 cgatccaaat tgcgttgaac tatctgaaaa ataccgattt tgatgcgatg gaagcagggg
     1321 tttacgaact caaaggcaga caaatttatg tgcaggtgtt ggatttgaac accaaatcga
     1381 aacacgaatt tcagccggaa gtgcatcgca attacctcga tgtgcagtat ctacaccgag
     1441 gcaaagagat aatggctgcg gcggtggata ccggcaccaa tccgattgcg atggaataca
     1501 accctgagcg ggatattcaa tattatcaat ccgttgccaa cgaaaatgaa tttcgctgcg
     1561 ttgaaggcaa tttttgacgg cacggaaacc attcgcaaag tggtggtaaa aattgcgatg
     1621 agcgaaattg aggagcaacg atgaaagcct tagctcaatt agtcggaaaa ctgttagaaa
     1681 tcttggtggt ggtgatttta tccgcaatgt cgattttggt tttcgtcaat gtggtactac
     1741 gttacggttt taacagcagc attagcgtga ccgaagaggt gtctcgctat atgtttgttt
     1801 ggttggcgtt tttaggggcg gtgttggcat ttagcgaaaa tcaacacgtt aaagtcacaa
     1861 tgctgaccga taaattatcg ccaacggctc gtaatatctt aagcgtagtc acagatttag
     1921 cgatgttgtt ctgttgctac ttaattgtgg acggtagctg gattcaattt aacctcaacc
     1981 tgaataactt tgccccgatt tcaggcttac cgcaaggcat cactttcctt gccagcacag
     2041 tggcaggggt attaatcggt ttattaatta ttgcccgcat tgtggcaacg gttggcgtga
     2101 ttgcaaaagg agaaaaatag atgactgtgg taatttttct ttccgtcttg cttggctcga
     2161 ttttattggg cattccggta gctttcgccc tgttggtctg cggtgtggcg ttaatgttgc
     2221 atttagatct gttcgatgcc caaattttgg ctcaacaaat tgtgagcggt gcagacagtt
     2281 tctcgctaat ggcaattccg ttcttcattc ttgccggtga aattatgaac gagggcggtc
     2341 tctccaaacg gattatcgac ctgccgatga aattagtcgg tcataaacgg ggcggtttag
     2401 gttttgtagc aattttagcg gcgatgatta tggcaagcct ttccggttcg gcggtggcgg
     2461 ataccgcagc ggtagtcgca atgctactgc cgatgatgaa aaccaccggt tatccgattg
     2521 accgctctgc cggtttaatc ggtacggcag ggattatcgc cccaatcatt ccgccaagca
     2581 ttccgtttat cgtatttggt gtggctagtg gcgtgtccat taccaaactg ttcttagccg
     2641 gtattttccc gggcattatg atggggatct gcttggctgc cctttggtgg tggcaggcaa
     2701 aacgtttgga tttaatgacc ttctccaaag cgaccaaaga agaactttgc atttcattta
     2761 aaaacagtat ttgggcgtta ttgttgcctg tcattattat cggtggtttc cgctcgggaa
     2821 tgttcacccc aaccgaagcg ggtgcggtcg caactttcta tgccttgatt gtttcactgt
     2881 ttgtttaccg tgaaatgaag ttcaaagatt tatacaaggt agtgttggcg gcagcgaaaa
     2941 ccacggcagt agtgatgttc ttagtggcag cggcaaatgt gaccggctgg ctgattaccg
     3001 tagcggaatt accgatgatg ctcaccgagc tgttagagcc gttaattgaa agcccgacca
     3061 cactcttatt ggtgattatg ttagcggtgt ttattatcgg aatggtgatg gacttaaccc
     3121 caaccgtgct gattttaacg ccggtattaa tgccgttggt ggaagaggca ggcattgacc
     3181 cggtttactt cggtgtgctg tttattctta acacctccat cggcttgatt acaccaccgg
     3241 tcggcaacgt gctaaacgtg attaccggcg tgtcgaaact gccgttcgac caagcggcta
     3301 agggcgtaat gccttacctc gtaatgatga tttgtttact actgttgttc gtgttcttcc
     3361 cgtcactggt gttagtgccg ttaagctgga tgcagtaatg gtttgaaccg aaacaagcgg
     3421 tcgaattgac gcaaaaattt gcaaattttt ttggcaaaac agaccgcttg caactcaatt
     3481 tttaatcaca ggagaaccct atgaaattat ttaatttaaa aaccttattt gccttagtgg
     3541 caggcgttgc ggtaatgagc ggctctgcaa tggcagaaac ctcactgcgt tttggttatg
     3601 aagccccacg ttccgatacc caacacatcg ccgccaaaaa attcgatgaa ttattggcag
     3661 caaaaaccaa aggtgaaatt aaattaaaac tgtttccgga cagcacctta ggtaatgctc
     3721 aaacgatgat cagcggcgtg cgtggcggta ctatcgacat tgaaatgtcc ggctcgccaa
     3781 actttaccgg cttagagcca aaactaaatg tgattgatat tccgtttatt ttcaaagatc
     3841 gtgaacacgt ttacaaagta ttagacggcg aagttggtca aggcttatta aaagatttag
     3901 aagcccaagg cttaaaaggc ttagccttct gggacgttgg cttccgtgct ttctccaact
     3961 caaaacaccc tgtgaccaag ccggaagaca tcaaaggctt aaaagtccgc accaaccaaa
     4021 acccgatgta cattcaagcc ttcaccttac taggcggcaa cccggtgcca atgccacttt
     4081 ctgagcttta caccgcactc gaaacccgtg ccgttgatgc ccaagaacac ccaatcggca
     4141 ttttctggtc ggcaaaactg tttgaagtac aaaaacattt aagtttaacc aaccacggct
     4201 acaccccgtt aatcgtggtg atgaacaaag cgaagtttga cggcttagct cctgaattac
     4261 aaaccgcatt agttgaagcc gcaaaagaag ccggtcaatt ccaacgtgat ctcaacgtga
     4321 aaaacgaaaa agagatcatc gaaaaattac gcaaacaagg catcgaagtg atcgaacaag
     4381 tggataacgc cccgttcaaa gcagcgattg aagcggaagt ccgcaaagct ttcgtggaaa
     4441 aacacggcga tgaattattg aaaaaagtgg atgcgttagc gcagtaattg actttatttt
     4501 agtctaaagc tccctccgtt tggggggagt tgttgtatag actctactat aggagagact
     4561 atgaaactgt ttaatttaaa aactttagcc gctttcgttg ccggttttgc actgttttca
     4621 accgcacaag cagaaacctc attccgcttt gcgtatgaag ctcctcgttc tgatactcaa
     4681 catattgcag cgaaaaaatt taatgatttg ctgcaagaaa aatcccaaaa agggctgaaa
     4741 ttaagcctct tccctgatag cacgctcggc aacgcacaaa cggcgattag cggtgtgcgt
     4801 ggcggtacta ttgatattgc aatgtccagc tcctctaact ttaccgggtt agaaccaaaa
     4861 ctgaatgtga ttgatattcc gtttattttc aaagacagag accacgccta taaagcctta
     4921 gatggagaga ttggtcaagg tttattaacc gcattagagg ctcacggttt gaaagggttg
     4981 gcgttttggg aagttggttt ccgagcgttt tccaactcca agcacccgat caccaaaccg
     5041 gaggatatta aaggcttaaa aatccgtact aaccaaaacc cgatgtatat tcaagccttc
     5101 tctattttag gtgcaaatcc ggtgccaatg ccgctttcca agctttacac tgcactggaa
     5161 accaaagcgg ttgatgccca agaacaccca atcggcattt tctggtcggc aaaactctat
     5221 gaagtacaaa aatatttcag ctttactaac cacggttaca ccccgttaat tgtggcgatt
     5281 aacaaagcga aatttgatgc actgccagcc gagcaacaaa ccgcattagt tgaagcggca
     5341 aaagaagccg gaaaatacca acgccaactt aacctagaaa atgagcagaa aattatcgac
     5401 tcgctcaaaa aagcaggtat tgaatttgtg gataacttag ataccacacc gttcaaagat
     5461 gccgtatcgg ctgaaacccg caaggcattt atcgagaaaa acggtgatgg cttagttaaa
     5521 gccattgatg cattggcgaa gtaaaaatag agctacaaag tgctcggtta aactaacctc
     5581 acacgaatcg tgtgaggtac aagcggtcaa aatccgcaaa atttttacac atctttcctc
     5641 aaaggaggaa caaatggcac agcccatttt aagtatgcag cacatcatca aacgctttgg
     5701 tagccatacg gcggtgaatg atgtcagcct tgatatccac tcaggcgaga tcgttgcctt
     5761 actcggtgaa aacggtgcag gtaaatcgac cctaatcaaa atccttgccg gtatttatga
     5821 ccgtgacgag ggtgatattc ttttccataa ccaaaaaatc caatctgccc acgctattga
     5881 gaaaggcaac aaaaagccca tcgcttttat ccaccaagat ttaggcctaa tagaatggat
     5941 gacgattgcc gaaaatatgg catttgtaat ggggttccct cgcaaatttg gcttgattga
     6001 ctgggcgaaa gtgcgagagc agtcgcaaaa agcccttgat tttgtaggca ttaaactctc
     6061 tgccgacact cgagtgtttg atttaagccg taccgaaaaa gccttactcg ccattgcccg
     6121 tgcgattgcc gtcaatgcag aaatcttggt tttagacgaa cctactgctt ccctacctgc
     6181 ctctgatgta gagcatcttt tcactgtgtt aaaccgctta cgttcccaag gtgtgggaat
     6241 gatttatgtg acccaccgtt tagatgaagt gatcgccatt tccgaccgca ttttggtgat
     6301 gcgggacggt ttcccggttg cagaagggca aaccaagctc tatgacgtca aaggcttggt
     6361 caaagcgatt gtaggcgaag agagccgtgg cagacagcgg gtggatttac caaccgatac
     6421 tcctgaaatt ttaagcctgc aaaatgtcgt ggttggcgac accggcccgg tgaatttccg
     6481 tctgcacaaa ggtgagatga ttgcccttgc cggcttgcgt ggagcagggc aggaagaaat
     6541 cggccgctta ttatttgggg agcgaacctt agacaaaggc gaaattcagt tagacaagcg
     6601 gtcatattct gctaaagatc cgcaagatgc gatagccaaa ggtgtggcat tggttgcggg
     6661 agaccgcacc cgtgaaagcg tgtttatgtc aatgactacc agtgagaacc tgtttatcaa
     6721 cccggtacta agcggtcatt caccattaaa acgctactcc cgccgtgaag agtggggtga
     6781 gagctggtac aaattccaac tattcgatat tcgtccgaaa aatttgttta ttgatgccag
     6841 tgcgttatcc ggtggtaatc agcagaaaat cgtgttggca cgttggatgc acctcaatac
     6901 gcctgttctg attttagaag acccgactgc cggcgtggat gtaggagcaa gggcagagat
     6961 ttacgacttg ctcaacaaag cccttgcaga aggggtcgca attattgtga tttccaccga
     7021 ctttgaagag atcgctcatt tgtgtaaccg agcattagtc ttcaaccgag gggaaattgc
     7081 cggtgaacta ctgaacgaag aggtgacttt cgccaatttg ctggcgttgg catcggatag
     7141 tcagtcgaaa aaacaataac ttactctcgc ccgcttgcgg gagagagaca gctaaaaata
     7201 gcgtcaggct atttttagca gagagagggt aaacaagcgg tcatttttct taaaaaatat
     7261 tgcgaaccct ctccctccga aagcctaacg gctttctcct ccctctcccg caagcgggcg
     7321 aggggaaaat gcagaatttt aacggagtaa tttatggctc aaacaaacat caaatcaacc
     7381 gcactggaac aaccggtatc tatcgcccgt gacggtttta tggcatggtt ttcacaaatg
     7441 tccactcgtt atggcttact gtggttatgt gtgttattgg tgattatttt ctcacttacc
     7501 accgaatctt tcgcctcaat gctgacatta aatgcgattt tagaaagtaa atcgaaaatt
     7561 gccctacttg ctttggcagc cacaactaca atgattgtcg gcaaaatcga cctgaacgtg
     7621 ggtttcggta ttgtgttgtg gcacattctt gtgattactc tgcaagtcca atacggcttc
     7681 tcgtggtata tggcgatctt aatcgtgtta gtgattgccg ctctttacgg cttgctgaat
     7741 gggattttag ttgctcttgc tgatatcgat agctttgtgg caacacttgg ctcaggcaca
     7801 gtgctttatg ccgtagcact ctggcactcg ggcggtcgcc aaattgtcgg ggatttaccc
     7861 gatgaattta tcgcactcaa cagcacagaa attttcggta tcccgatttc tgctttctat
     7921 gtgctggcag tggctatcgt aatgtggttg gtgacagaac ataccccgac cggtcgttgt
     7981 atgtatgcgg ttggtggcaa cccaacggca gctcgcctaa atggtatcaa tattaaaaaa
     8041 tacaccattg tgccgtttat tgtctctagt gtaatcactg ctttcacagg cgtattgatc
     8101 tctgctcaac aaggcgttgg tcaggcaagt gtcggtatgg actacttact gcccgcattg
     8161 gttggggcat tcttagggag taccactatt cgcccagggc gtattaacgt atggggaaca
     8221 gtggtgggga ttgccattct tgccatcggt atttcaggta ttcaacaatt cggtggagcg
     8281 ttctgggttg agccactctt taacggtgca acactgttac tttccatcac tattgcaggc
     8341 tatgcccaac gcaaacgctt gttaaatcaa aaagctgtac agaaaaaatc gaccaactaa
     8401 gaggatttga ttatgaaaaa agcatttcgt acaaccttaa ctgcaatcgc aaccttaact
     8461 gctattgctt tcggaaataa tgcgtttgcc aatgacgacc cgttcgtaca agaagcaaaa
     8521 cagcttgtcg cagcagctac ggctaagcaa gaaaaatggg acggaccgac aaccggccct
     8581 caattacaaa aagagaaaac tgtgattttt attgcctctg atatgaaaaa cggtggtgta
     8641 cttggtgtga ttgacggaat gaaagaagct gttggcgtaa cgggctggaa gttagatgta
     8701 ttagacggtg caggtacggt aaacaaccaa ctcgccgcac ttaaccaagc gattgcccgc
     8761 aaaccggatg cgattgtgat tggtggctgg aacccgaatg tggcgaaaat cccactacaa
     8821 aaagcgacta aaaacggcat taaacttgca gcttggcacg caacaccgga gccgggtgcg
     8881 attgataaat acggcatttt ctacaacgtg acctctgact cagacgaaat tgcacgctta
     8941 tcggcaatgc ttgcggtggc agaatctaac ggtaaggcaa aagcgattat tttaaccgac
     9001 tcgctatatg aaattgcgtt acgcaaagcg aacgtcatga aagaagttat cgaaaaatgt
     9061 aaagagtgta aggtgttaga gtttatcgac acgccattgg cggatacctc aagccgtatg
     9121 cctaacctca ctttcagctt actgcaaaaa tatggcgacg atttagagta tgcgttggca
     9181 attaacgatc tctactttga ctttatggct ccgtctttac gcaccgctgg taaagataaa
     9241 ccgtttagca tttccgcagg tgatggctca gtaacggctt atcaacgtat tcgcaatggt
     9301 gataaacagt ttgcaaccgt ccctgagcca cttaacttac acggctggca gttagtcgat
     9361 gagctaaacc gtgctttcgc taacgaaaaa ccgtctggtt atatcacacc ggctcaccta
     9421 gtgactaaag aaaatgtggc gtttgacggt ggtgaaaaca acctgtatgc cccacaaaac
     9481 ggttacaaag gcgcttacaa agcgatttgg ggcgtgaagt aatgacgtag gggcaaataa
     9541 atatttgcct cgaacaagcg gttgtttttt gcaaagattt tgcgaaattt aaccgcttgt
     9601 cataaaaaat agagagggta atacaaatga gcaataaatt tatccccaaa atctccaacc
     9661 ctcgttttaa tgagctggtt gggaatacgt ttgaacttga agagcttttt gataaggcaa
     9721 tctgggcaga agggccggcg tggagcaagc cacaacaagc cctctatttt agtgatgtaa
     9781 aaggttgcac aatgtaccgc tggacagagc aagatggtac acaggttttc cgccagccgt
     9841 cttatttttc taacggcaat gcgtttgatg caaacgacaa cttgattacc tgtgaacacg
     9901 gcaatcgagg tatcagttta accacgccaa gcggtgaata tcgttttttg gtcggtcaat
     9961 tagacggaaa acgactgaac tcacccaatg atgtggtggt aaaatcagac ggcatcattt
    10021 ggttcactga tccaccttac ggtattttgt cggataacga aggcaaacag tcgccaagcg
    10081 aaattatcgg ttgttatatt tactgctatg atccgaaaac cgacaagctg aatattgcaa
    10141 ctttcaacac gatgcgacca aacggcttgg ccttctcgtc agacgaaaaa caactctttg
    10201 tggcagatat gtcgagcgtg gaatttgacg aagatggctt acaccactta gtcgtatttg
    10261 atgtggacgg taataaacta aaaaatcgcc gagatgtggc ggaaattagc cccggcattc
    10321 cggacggctt ccgaattaaa caaaacggcg taattttctg ctcttgtgaa gagggaatta
    10381 ttgctctttt acctgacggc acggagctgg gacgatttgt gctgggtaag ctaacctcaa
    10441 attgtagctt tggcgatgat gaacaaacac tgtttattac ttgttcgaat agcgtttatc
    10501 gtttaacaat taaatagcag aatgcattaa gtgcgtctgt tgtagcatta caagcggttt
    10561 ctttttcaaa attttttaca aacaaataat ggataacaaa atgaactaca cactcggcat
    10621 tgattgcggt gggactttta tcaaagccgc tctttttgat acacaaggta atattcaagc
    10681 ccttcatcgt gaaaacgttt cggtaatcag tgaacaggca ggttatgcag agcgtgatat
    10741 gtcacaactc tgggctattt gtgcggaagt agtgcgttca accattgaaa aaagtggggt
    10801 aaacccagcg gatattaaag cagtagggat ttccgctcaa ggcaaagggg cgtttttact
    10861 ggataagcag aatcgaccgt tggggcgtgc gattttatct tccgaccaac gttcccttga
    10921 gattgttaaa caatggcagg ctgaaggcat tccgcaacaa ctttacccga ttacccgcca
    10981 aactctttgg acaggacacc ctgtgtcgat tttgaagtgg gtgcagcaaa atgaaccgga
    11041 gcgttatgca caaatcgggg cggtgttgat gtcgcacgac tatttgcgtt tttgcttaac
    11101 cggtgagttg cattgcgaag agaccaacat ttccgaaagc aatctttaca atatggaaaa
    11161 aggcgaatac gaccaacagt tagccgattt gttgggaatg ccggatgtgt tggagaaatt
    11221 accaccagtg attgaaccga ataaaatttc gggatatgtg actgaaaatg ccgccaaatt
    11281 aaccggttta gcggtgggta cgccgatagt cggtggcttg tttgatgtgg tttctaccgc
    11341 acgttgtgcc aatttagatg acgaaaccaa actgaatgca gtgctgggaa cgtggtcggt
    11401 ggtgagcggc attaccgatc atattgatgc aagccaatca ttaccttttg tttatgggcg
    11461 ttatgccgag gcagggaaat ttatcgtcca cgaagcaagc ccgacttctg ccggtaattt
    11521 agagtggttt gtgaatcagt ggaaactcga ttatgtgaaa atcaaccaag ctgttgccca
    11581 attaccgccg gcggcaagct cggtgctgtt tgtgccgttt ctgtatggct caaatgcagg
    11641 cttaggaatg caagccggtt tctatggtat gcaagcccac catacccaag cccatctctt
    11701 gcaggcaatt tacgaaggag tgttgtttag cttgatgagc catttaaacc gtatgtttat
    11761 ccgctttcca aaggctgacg tgctgcgagt taccggcgga ccggtgaaat ctaaagtttg
    11821 gatgcaaatg ttggccgatt taaccggaat gcggcttgaa ataccacaaa tggaagaaac
    11881 cggctgcttg ggtgcggcat taatggcaat gcaaggggtt gggctggata taagccaagt
    11941 tcaagcccta aataatgaaa tgcaggtgtt tgagccaaat ccggaaaatt atgcggctta
    12001 tcagacgaaa tatcagcgtt accaaaaatt aactgaagca ttaaatacaa tgatttaacc
    12061 tgaatttatg cttttacaag cggtaatatt tggttacttt tttgcaaaaa ttactgcttt
    12121 tttgtttatt taatttatga attta
//
LOCUS       DANLYI010000058        11954 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.058, whole genome shotgun sequence.
ACCESSION   DANLYI010000058 DANLYI010000000
VERSION     DANLYI010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 11954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11954
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.058"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            93..590
                     /gene="purE"
                     /locus_tag="PXD81_001171"
     CDS             93..590
                     /gene="purE"
                     /locus_tag="PXD81_001171"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HDL5537204.1"
                     /translation="MPNKAEIAIVMGSKSDWATMSEATQILDQFGLSYHVEVVSAHRT
                     PDKLFSFAETAETNGYKVIIAGAGGAAHLPGMIAAKTIVPVLGVPVKSSMLSGVDSLY
                     SIVQMPKGIPVGTLAIGPAGAANAGLLAAQILAAFNPDIAEKLRIFRQTQTQMVLDNP
                     DPRVE"
     gene            605..1570
                     /locus_tag="PXD81_001172"
     CDS             605..1570
                     /locus_tag="PXD81_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005607504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HDL5537205.1"
                     /translation="MSKKLLILHTGGTISMKEGEDGKVSPSNENPLLAALERLNHPAK
                     LAQESLFNIPSPHINIEHWQQLKMRIEKAVNEENVDGVVITHGTDTLEETSYFLDLAL
                     SVNIPVVITGAMRSSNELGADGLINLQSAILVALNEESRDKGVLVVMNDEIHNAKFVT
                     KTHTTNVATFQTPTFGPCGLVTKESVIFFQKLTTYERYPIQQVTKANVQLLKAYAGMD
                     SFLLEQLAKSKCDGVVIEALGAGNLPPSCLAGIEALVNANIPIVIVSRAFNGITQDVY
                     DYVGGGKQLKQKGIIFTKGLSGQKARIKLMVLLSQTLDKPLSDYF"
     gene            1589..2677
                     /gene="purK"
                     /locus_tag="PXD81_001173"
     CDS             1589..2677
                     /gene="purK"
                     /locus_tag="PXD81_001173"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012262901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HDL5537206.1"
                     /translation="MQKSAIYPPVYVLGNGQLGRMLRYAGAPLDIEVKPLAFDAPVFD
                     IEPNSIITAEIERWADTPLTQLLGNHPNFVNLNVFGKLADRFTQKSLLDELHLATSKW
                     QLLENAEHWQDIFQHIGEKVVVKRRTGGYDGRGQWIVTQDSIHQITPELFGEVIAEKF
                     IPFEGEISIVGARFRDGSTRFYPISHNLQQNGILRYSVSDVNLPNQALYQQQAEQMLA
                     TVMQKLEYVGVMAMECFVVGDKLLINELAPRVHNSGHWTQLGCAISQFELHLRALLDL
                     PTPALDQIAPSVMVNLIGIEHNKQWLSLPFSQLHWYGKEVRAGRKVGHINLTHTDKNG
                     LIELLEQLRPSLTEDFHSGLDWAINKLK"
     gene            complement(2898..3308)
                     /locus_tag="PXD81_001174"
     CDS             complement(2898..3308)
                     /locus_tag="PXD81_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5537207.1"
                     /translation="MISRSLFSKLILFAKIVIFSQLVFMVVIWIAHHIMREYSNPFER
                     FGTLFNNVFLTFIIPMMVSSAVIVYKGYTQKNEDKVFWIYMGFFLIWILGFIYFEPAV
                     YDKSLKISGAGFGAMLSIIICSKLLPKETNKEQL"
     gene            complement(3311..5329)
                     /gene="uvrB"
                     /locus_tag="PXD81_001175"
     CDS             complement(3311..5329)
                     /gene="uvrB"
                     /locus_tag="PXD81_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009874701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HDL5537208.1"
                     /translation="MSKQGKPFILHSPFKPSGDQPSAIAKLTEGLNDGLAHQTLLGVT
                     GSGKTFTIANVIAKLNRPAMVLAPNKTLAAQLYAEMKAFFPENAVEYFVSYYDYYQPE
                     AYVPASDTFIEKDASINEQIEQMRLSATKSFLERRDTIVVASVSAIYGLGDVDAYMQM
                     MLHLQVGAMIGQREILSRLAELQYTRNDQAFQRSTFRVRGDVIDIYPAESDEVALRVE
                     LFDDEIENLSLFDPLTGHSLGKVPRYTIYPKTHYVTPRERILEAIEQIKVELADRRTY
                     FIKENKLLEEQRIAQRTQFDIEMMNELGYCSGIENYSRYLSGRKEGEAPPTLFDYMPA
                     DGILVIDESHVTVPQIGGMYRGDRARKETLVQYGFRLPSALDNRPLRFEEFERLSPQT
                     IYVSATPGAYELEKNPDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIHKRVAVDER
                     VLVTTLTKKMAEDLTDYLDEHGVRVRYLHSDIDTVERVEIIHDLRMGMFDVLVGINLL
                     REGLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNLNGKAILYGDRITNSMQKA
                     ITETERRREKQMKYNEEHGITPQALNKKVGELLDIGQSDKPKRGKQAVKKAENSATDY
                     KPKSRKELEKELKTLEQQMRDFAKDLEFEKAAAVRDKLQHLKAYLLEV"
     gene            complement(5568..5804)
                     /locus_tag="PXD81_001176"
     CDS             complement(5568..5804)
                     /locus_tag="PXD81_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HDL5537209.1"
                     /translation="MSRTTSVTLAEPLSQFVNQMVESGRYGSTNEVISAALKILEAQE
                     QQLIQLRALIDEGLESGVSEETVQSIISKAKRNV"
     gene            5967..6812
                     /locus_tag="PXD81_001177"
     CDS             5967..6812
                     /locus_tag="PXD81_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005823485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EfeM/EfeO family lipoprotein"
                     /protein_id="HDL5537210.1"
                     /translation="MFLLKGNFYAFNQTCFCTFALSGFFVATQAAALDLTKETEAYKQ
                     FVVEQIDQLVTDTEKFVGYLNKGDVQKAKKIYPLARMYFERSEPIAESFGDLDPRIDA
                     RLADLAEEGKTEKDWSGFHKIEKVLWQKNTTKGTKATAEQLLKDVKELRAKIPTAEVT
                     PEPMITGAVDLLNEVSTTKVTGEEEIFSKTDLYDFKANIEGAEKIYEIFKTQLEQIDA
                     KLSTEIGSRFAAVNALLAKHNKSKTGYDYVPYNKLSKADIKALAEAVNQLGEPLAQLG
                     VLLNK"
     gene            6823..8019
                     /gene="efeB"
                     /locus_tag="PXD81_001178"
     CDS             6823..8019
                     /gene="efeB"
                     /locus_tag="PXD81_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005614931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deferrochelatase/peroxidase EfeB"
                     /protein_id="HDL5537211.1"
                     /translation="MMAETTSRRDFLKHTALVGAGLLSAPAFALESRQISEKSQNQYD
                     FYGKHQAGIVSPAQKHIYFLVLDLDTTDIAKVKTLFQNWTEYSRNLTLGKNVKPYSAN
                     AYVPPADTGEADSLSAQNLTLTFGVSANFFTKLGIEKHKPKELNDLPHFPRDQLQAEY
                     TGGDICIQACADDPQVAFHAVRNLVRVARGEIKMKWSQMGFNSFANQDTPRNLFGFKD
                     GTANAESLKDQENTVWVQQEGWLKGGSYLAVRRVKMFLETWDRTHLQSQEETFGRHRD
                     SGAGIGHQHEFDKLDLAKKDEKGNAVIPEVSHAHLANKTGLKILRRSFSYSSGIDDKG
                     QFDSGLLFVSFQQSPEQFIKIQNALGNVDKMNEYITHIGSGLFACFAGVKDENDYLGK
                     ALFDLI"
     gene            8030..8649
                     /locus_tag="PXD81_001179"
                     /pseudo
     CDS             8030..8649
                     /locus_tag="PXD81_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596976.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+/Pb2+ permease"
     gene            complement(8685..9503)
                     /locus_tag="PXD81_001180"
     CDS             complement(8685..9503)
                     /locus_tag="PXD81_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HDL5537212.1"
                     /translation="MKYDLHAHSTASDGVLSPTELVQRAVEQGVTMLALTDHDTISGI
                     QEAKNFAQNQPLELISGVEISILWQEKSIHLAALNIDENNEELVKFLDNQAQLREERA
                     VEIGEKLAKVGIANAYEGAKALATGEVTRAHYGRFLYEQGYVRNIEHAFKRYLGMGKP
                     AYVKPRWSSLEDAVNVTHTSGGVISIAHPLRYKMTARWIRRLIADFKQAGGDGIEVSG
                     CGQTPDQRQLLARWAKEFDLYGSVGSDFHYPTGWIELGKSLELPQECKPIWTLF"
     gene            complement(9515..10114)
                     /locus_tag="PXD81_001181"
     CDS             complement(9515..10114)
                     /locus_tag="PXD81_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA glycosylase"
                     /protein_id="HDL5537213.1"
                     /translation="MTMDNLPLEQHPFDPILPEQATVVMVGTFPPTSEKRCMEFHYPN
                     FQNDMWRIMGNIFYADPNYFRVGEEKRFDPIRIEAFLREKGVALCSSAKTAIRLKGNA
                     SDKDLKIVDPIDMDELLLKLPKLKWLFTTGGLATDTLLGLLPEKHKAPKTNEWVVYPY
                     SATRELYLYRLPSTSRAYPLSLANKTEAYRQFFRTAGLI"
     gene            complement(10111..11658)
                     /gene="sbcB"
                     /locus_tag="PXD81_001182"
     CDS             complement(10111..11658)
                     /gene="sbcB"
                     /locus_tag="PXD81_001182"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HDL5537214.1"
                     /translation="MSDKFSFFFYDYESFGVSPALDRPAQFAGIRTDADFNIIGEPVM
                     FFCKQTPDYLPSPEAVMVTGITPQQCNAEGVSEPEFAARIHAEFSRANTCTIGYNNIR
                     YDDEMTRYTFFRNFYDPYEYSWKNGNSRWDLLDLVRACYALRPDGIEWPTDETGMPSF
                     KLENLTKANGIAHENAHDAMSDVYATIAMAKLIKQKQPKLFDFFFNLRSKKEVEKLID
                     TGEMTPLVHVSGMLGNYRGNTAWVVPLAWHSTNQNAVIVCDLSGDMNGLLTENAEVLR
                     QRLYTKRDELAENELPVPLKLVHINKCPILAPAKTLLPENAERLGIDRALCLENLKTL
                     KAQKSLIREKVIEIFNEERTFEPSTNVETTLYDGFFSPADKNNMAILRTLPPEDLANH
                     GLKFEDPRVEALLFHYRARHYPQTLSRAEQIKWQKHCNQQIEAKAAQFAQSIDDLFQQ
                     HHDNPEKVKLLENLTAYAEQISQQQAVIYRQNVAKDEKLLSELNRVAEQPLDKTEKLK
                     MLKELIK"
BASE COUNT         3498 a         2605 c         2486 g         3365 t
ORIGIN      
        1 aaaattgctt ttcacttctt aaatagctac gcaaacgttt gctttgtatg ctatgattac
       61 actgttttac attagtatca taaggattta aaatgccaaa taaagctgaa attgcgatcg
      121 taatgggatc taagagtgat tgggctacaa tgtctgaagc aacccaaata ttagaccagt
      181 tcggtttgag ctatcacgtt gaagtcgtct cagcacaccg cacaccggac aaactctttt
      241 cttttgctga aactgcagag actaacggct ataaagtgat tattgccgga gccggcggtg
      301 cagctcactt gcccggaatg atcgctgcta aaaccattgt gccggtgtta ggtgtgccgg
      361 taaaaagctc aatgttaagt ggtgtagata gcctctattc gattgtgcaa atgccgaaag
      421 gtattccggt cggtacgctc gcaatcgggc cggcaggtgc agcaaatgca ggcttattag
      481 ccgctcaaat tttagcagcc tttaaccctg atattgcaga gaaactgcgt attttccgtc
      541 aaacccaaac tcagatggta ttagataatc ctgatcctcg tgttgaataa ctattgtata
      601 aaagatgtct aaaaaattat taatcctcca cactggtggt actatttcaa tgaaagaagg
      661 ggaagacggt aaggtctccc cttccaatga aaatcctctc ttggctgcct tagaacgttt
      721 aaaccaccca gcaaaattgg cgcaagaatc gttatttaat attccctctc cgcatatcaa
      781 tatcgagcat tggcaacagt taaaaatgcg gattgaaaag gcagtgaatg aggagaatgt
      841 cgatggcgta gtaatcaccc acggcacaga taccttggaa gaaacctcct attttctgga
      901 tttagccctc agcgtaaata taccggtagt aattaccgga gcaatgcgtt ccagtaacga
      961 actgggtgct gatggcttaa tcaatctaca aagtgcgatt ttagtggcgt taaatgaaga
     1021 aagccgagat aaaggcgtgt tagtggtgat gaatgatgaa atccacaatg ctaaatttgt
     1081 gaccaaaacc cacaccacta atgtggcgac tttccaaacc ccaacctttg ggccttgtgg
     1141 cttagtaacg aaagaatctg tcattttttt ccaaaaattg accacttacg aacgttatcc
     1201 aattcaacaa gtaactaaag ctaacgtgca actactgaaa gcatacgccg gaatggatag
     1261 ttttctgctg gaacaattag cgaaaagcaa atgcgatggt gttgtgattg aggcattggg
     1321 tgcaggtaat ttacccccta gttgtctggc tggtattgag gctttagtga atgccaatat
     1381 tccaattgta attgtttctc gggcattcaa tggtattacc caagatgtct atgattatgt
     1441 aggcggtgga aaacagttaa agcaaaaagg cattattttt accaaaggct taagtgggca
     1501 aaaagctcgc attaaactta tggtcttact cagccaaacc ttagacaaac cactatcaga
     1561 ctatttctaa cccattgaga ataccacgat gcaaaaaagt gcaatctacc cacctgtgta
     1621 tgtattaggc aacggccagc tcgggagaat gctacgctac gccggagcac cacttgatat
     1681 tgaagtcaaa ccgctggctt tcgatgcccc tgtgtttgat attgagccaa acagcattat
     1741 taccgcagaa atcgaacgtt gggcagatac gcctttaacg caattactcg gtaaccaccc
     1801 gaattttgtg aacctgaatg tttttgggaa gttagccgat cgttttacgc aaaaatcact
     1861 acttgatgaa ctccatcttg cgacttcaaa atggcaactg ctagaaaatg ccgaacattg
     1921 gcaagatatt tttcagcata ttggcgaaaa agtagtggtt aaacgccgaa ccggaggata
     1981 tgacggacgt gggcaatgga ttgtaacgca agactcgatt catcaaatta cacctgaatt
     2041 atttggcgaa gtgattgccg aaaaatttat cccgtttgag ggggaaattt ccattgttgg
     2101 ggcgagattc cgtgacggct caacccgttt ctaccctatt tctcacaatt tacaacaaaa
     2161 cggcattcta cgttactccg tctccgatgt gaatttaccg aaccaagccc tctaccagca
     2221 gcaagccgaa cagatgctcg ctacggtaat gcaaaaatta gagtatgtcg gcgtaatggc
     2281 aatggaatgc tttgttgtgg gcgacaagct actcatcaac gagcttgccc ctcgagtgca
     2341 taacagcgga cactggacac aactcggctg tgccattagc caatttgagt tacatttacg
     2401 ggcattatta gatctaccta cgccagcact cgaccaaatt gccccaagcg tgatggttaa
     2461 tttaataggc attgaacata acaagcagtg gctaagcctc ccatttagcc aactacattg
     2521 gtatggcaaa gaagtccgag ctggtagaaa agttgggcat atcaacctca ctcacaccga
     2581 taaaaacggc ttaattgaac tactggaaca actcagaccg agcctaacgg aagatttcca
     2641 ttcgggatta gattgggcaa tcaataagct caagtaagcg gtcggatttt gtaacttttt
     2701 tgcaaatctg tttacccgat ggtgcgagct tcctcgctcg taccaaacaa gcggtcgact
     2761 tagtgctaaa atttgcaaaa taattcaaca taatggctac ttccggtcat catcttgatc
     2821 tgaaaacccc tcagggtgga tatggctatc aaaaagcatt ttcttcctcc gagaaaacag
     2881 atttcttcac aagaatctta gagctgctct ttatttgttt ccttaggcaa taacttcgag
     2941 caaatgataa tagagagcat agccccaaat cctgcacctg aaatcttcag tgatttatca
     3001 taaactgccg gctcaaaata aataaatccc aaaatccaaa ttaaaaagaa ccccatataa
     3061 atccaaaaca ctttatcttc atttttctgg gtataacctt tataaacaat aaccgcacta
     3121 cttaccatca tcggaataat aaaagtcaaa aacacattat tgaacagggt accaaatcgt
     3181 tcaaacggat tactgtactc ccgcataata tggtgggcaa tccagataac caccataaaa
     3241 acaagttgag agaaaataac aattttggca aataagatta attttgaaaa taatgaacga
     3301 gaaatcatat ttaaacctcc aacaaatacg cctttaaatg ctgcaactta tcccttaccg
     3361 cagccgcttt ttcaaattcc agatctttcg caaaatcacg catttgttgt tccagcgttt
     3421 taagctcttt ttccagttct ttacgggatt tcggcttgta gtcggttgca gaattttcgg
     3481 cttttttgac cgcttgtttg ccccgttttg gcttgtcgga ttgtccgatg tcgagcaatt
     3541 cacccacttt tttgttcaac gcttgtggcg tgatgccgtg ttcttcgttg tatttcatct
     3601 gcttttcacg gcggcgttcg gtctcagtaa ttgctttttg cattgagttg gtaatgcggt
     3661 cgccgtataa aatcgctttt ccgttgaggt ttcgggcggc acgtccgatg gtctgaatta
     3721 acgagcgttc ggaacgtaaa aatccctctt tatccgcatc aagaatggca accagcgaca
     3781 cttcgggcat atctaagccc tcacgcaata agttgatccc caccagcaca tcaaacattc
     3841 ccattcgtaa atcgtggata atttcgaccc gttccaccgt gtcaatgtcg ctatgtagat
     3901 aacgaactcg tacgccatgt tcgtccaaat aatcggtgag atcttccgcc atttttttgg
     3961 tgagtgttgt caccagcacc cgttcgtcca ccgccactcg tttgtgaatt tcagacagta
     4021 aatcgtccac ctgcgtagca acggggcgaa cttcgataat cggatcgagc aagccggttg
     4081 gacgaactac ttgatccacc acatcgggat ttttctccaa ttcatacgcc cccggtgtgg
     4141 cagaaacgta aatcgtctgt ggtgaaagac gttcaaactc ttcaaatctc aacgggcggt
     4201 tatccagtgc agacggcaga cggaaaccgt attgcaccaa tgtttctttt ctcgctcggt
     4261 cgcctcgata cattccgcca atttgtggca cagtaacgtg gctttcatca atcaccaaaa
     4321 taccgtccgc cggcatataa tcaaacaggg tcggaggggc ttcgccctct tttctgcccg
     4381 agagatagcg tgagtagttt tcaatgcccg aacagtagcc cagctcgttc atcatctcaa
     4441 tatcaaactg cgtacgttgg gcgatccgct gctcttccag caatttattc tctttgatga
     4501 aataggtgcg acggtcggca agctcgacct taatttgctc gattgcctcc aaaatccgtt
     4561 cccgaggggt cacatagtga gttttcgggt aaatggtata gcgaggcacc ttgcccaagc
     4621 tatgccctgt aagcggatca aacagcgaca gattttcgat ttcatcgtca aacagctcta
     4681 cccttaacgc tacttcatcg ctttcggcag ggtaaatatc aatcacatcg ccccgcactc
     4741 ggaaagtcga acgttggaac gcctgatcgt tgcgagtata ttgcaattcg gcaaggcgag
     4801 agagaatttc acgctgccca atcatcgccc ccacttgcaa atgcaacatc atctgcatat
     4861 aagcgtccac atcacccaag ccgtaaatgg cagaaacgga cgccactaca atcgtatctc
     4921 ggcgttccaa aaaggatttg gttgcggaaa gtcgcatttg ctcgatctgc tcattgatag
     4981 aggcatcttt ctcgataaag gtatcgcttg ccggcacata ggcttcgggc tggtagtaat
     5041 catagtaaga gacgaaatac tccaccgcat tttccggaaa gaacgctttc atctcggcat
     5101 aaagctgggc ggcaagggtt ttattcgggg caagcaccat cgccgggcga ttgagcttgg
     5161 caatcacatt agcaatggta aaggttttcc ccgagccggt tacgccaagt aaggtttgat
     5221 gtgccaagcc gtcatttaag ccttcggtca atttggcaat cgcacttggt tggtcgccgg
     5281 agggcttgaa aggagaatgg aggataaagg gtttgccttg cttgctcata gaaaatcctg
     5341 tcttaaaaaa tcggtttatt ttagcacaag caagcggtcg tttttatcgg gatttttgca
     5401 aatttattgt aactgcttat attgatccat tcttggatct aacaccctaa caataaagat
     5461 accttttggg attggtttat aataaataga atgtgagcgg tggtaatgac agcgtaacaa
     5521 agggcgaata aagtaacgct aaatctcgct ctgccctaac agaaagttta tacattacgt
     5581 tttgccttgc tgataataga ttgaacggtt tcctcagaaa caccgctttc taagccttca
     5641 tcaattaagg ctcttagctg aattagttgc tgttcttgtg cttccaatat ttttaatgcg
     5701 gcactgatga cctcgttggt cgaaccataa cgcccacttt caaccatttg gttcacaaat
     5761 tggcttaatg gctcagctaa agttacgctg gttgttctac tcattactat tcctatctta
     5821 ttgattattg agcagattgt aatggctcta atttagataa gcaagcggtc tatttttagc
     5881 aataatttgc aaaacaaaat gtgatttaca tcacaaaatc agcattattt tttgcgaata
     5941 attcgcaata aggatattat gtcgcaatat ttttattaaa aggaaatttc tatgcgttta
     6001 accaaacttg cttttgcact tttgcacttt ctggcttttt tgtggctact caagcggcag
     6061 ctttagactt aactaaggaa accgaggctt ataaacaatt tgtggtagaa cagattgacc
     6121 aactggttac cgatacggaa aaatttgtcg gctacttaaa caaaggcgat gtgcaaaaag
     6181 cgaagaaaat ttacccgctt gcaagaatgt attttgaacg ttctgaaccg attgcagaaa
     6241 gttttggcga tttagaccct cgtattgatg cccgtctggc agatttagcc gaagaaggta
     6301 aaaccgagaa agattggtca ggtttccata aaatcgagaa agtattgtgg cagaaaaaca
     6361 ccactaaagg cacaaaagca acggcagaac aattattaaa agatgtaaaa gagctgcgtg
     6421 cgaaaattcc cactgcagaa gttacccctg agccgatgat taccggtgcg gtagatttat
     6481 taaacgaagt ttctactact aaagtaaccg gtgaagaaga aattttctct aaaaccgacc
     6541 tttacgattt taaagccaat attgaaggtg cagaaaaaat ctatgagatt tttaaaacgc
     6601 aattagagca aattgatgcc aaactctcaa cagaaatcgg ctctcgtttt gcggctgtca
     6661 atgcgctgtt agcaaaacac aataaatcta aaaccggtta tgattatgtg ccttataaca
     6721 aactgtcaaa agcggatatt aaagcgttag cagaggctgt aaaccaatta ggcgagccgt
     6781 tagctcaact tggcgtactt ttaaataaat agtggagcaa aaatgatggc agaaaccact
     6841 tctcgccgtg attttttaaa acataccgca ttagtaggag caggcttatt gtctgctcct
     6901 gctttcgctt tggaatcacg acaaatatct gaaaaatctc aaaatcaata cgacttttac
     6961 ggcaaacatc aagctggtat tgttagccct gcacaaaaac atatctactt tttagtgctg
     7021 gatttagaca ccactgacat tgccaaagta aaaacattgt ttcaaaactg gacggaatat
     7081 agccgtaacc tcacgcttgg caaaaatgtg aaaccttata gtgctaatgc ttatgttcca
     7141 ccggcggata ccggtgaggc agatagcctt tcggcacaga atctcactct tacttttggc
     7201 gtcagtgcca atttctttac aaagctcggc attgaaaaac ataagccgaa agaactgaat
     7261 gatttaccgc atttccctcg tgatcaacta caagcagaat acaccggtgg cgatatttgc
     7321 attcaagcct gtgcagacga tccgcaagtg gctttccacg ctgtgcgtaa tttggtgcgt
     7381 gttgcccgtg gtgaaatcaa aatgaaatgg tcgcaaatgg ggtttaactc ctttgccaat
     7441 caagacacac ctcgtaactt attcggtttt aaagacggca ctgccaacgc agaaagttta
     7501 aaagatcagg aaaatacggt atgggtgcaa caagagggct ggctcaaagg cggttcttat
     7561 ttagccgttc gccgtgttaa aatgttcttg gaaacttggg atcgcaccca tctccaatcc
     7621 caagaagaaa ccttcggtcg tcaccgtgat tccggagccg gaatcgggca tcagcacgaa
     7681 tttgataaat tagatttagc caaaaaagac gaaaaaggca atgcagtaat tccggaagtt
     7741 tcacacgctc atcttgccaa taaaacgggg ctgaaaatcc tacgccgttc attctcctat
     7801 tcaagcggta ttgatgataa agggcaattt gattcagggt tgttgtttgt ctctttccaa
     7861 caaagtccgg agcaatttat caaaatccag aacgctctcg gcaatgtgga taaaatgaat
     7921 gaatacatca ctcatatcgg cagtggttta tttgcctgtt ttgccggtgt gaaagatgaa
     7981 aatgactacc ttggcaaagc gttgtttgat ttaatttagg gaaaaggaaa tgaaattaaa
     8041 agcattagtg ctaattccgt tactcttttc gagtacagga ttgcttgcca aagtgaatgt
     8101 cagcccctta tttgttcagc tttccgatgc aatggcagag agtaaaaaag gggaatttgc
     8161 aaaatccagc caaaatttga ccgctttaca gcacgatttt gagcagatta gccagcacga
     8221 ttccctcgcc ggagttgaag tacagaccgc tctgaaaaaa gcgattgaaa cacccaatgc
     8281 tgaaaactta gagagccttt ctaaaaactc tatgcgtttg aaaaagaaca aaaccctagc
     8341 ggttatgctg aaaaacatca ggattttgtg gcaaaaatga cgccgcttta tgcagaactt
     8401 gctcaaaccg tgccaactca agaaatcgcc aaaatcaaat gggcggctta tcagtttggt
     8461 aagaattggg taaaacagga aaaagcagta cgggaaatca gtcttcccca ttatggcaaa
     8521 tttgagcgta ttcttggttt aatgcgaatt aatctgaacg ctgaaaagcc ggatatggca
     8581 aaaatcagtg aactggtttc cgaactaggt gtggtaatgg cagactttaa gcaagttaaa
     8641 gtgaaatagt aaaaaatcgg ggcaaattat ttgccccgat aacactaaaa taacgtccaa
     8701 atcggtttac attcttgggg taattccaaa cttttaccga gctcaatcca gccagttggg
     8761 tagtggaaat cagaaccgac cgagccatat aaatcaaact cttttgccca acgggcaagt
     8821 aattgccgtt gatcaggtgt ttgtccgcag cctgatactt caatgccatc gcctccggct
     8881 tgtttgaaat ctgcgattaa acggcgaatc caacgagcag tcattttata gcgtagcggg
     8941 tgagcaatcg aaatcacacc gcctgaggtg tgggtaacat tcaccgcgtc ttctaaacta
     9001 ctccatctcg gtttcacata ggcaggtttc cccattccca gataacgctt aaaggcgtgt
     9061 tcaatattgc ggacatagcc ttgttcatac aaaaaacgcc cataatgtgc ccgagttact
     9121 tcccctgttg ccaacgcttt tgccccctca taggcatttg caatcccgac ttttgctaat
     9181 ttttcaccaa tttcgaccgc tctctcttcc cttaattggg cttggttatc caaaaacttc
     9241 accagctcct cgttgttttc atcaatattc aacgccgcaa gatgaatact tttttcttgc
     9301 cataaaatcg aaatttccac cccagagata agctcaagcg gttgattttg ggcaaaattt
     9361 ttcgcttctt gaatgcccga aatcgtatcg tgatcagtta aagctagcat tgttacccct
     9421 tgctctaccg cacgttgtac caattcagtg ggagaaagta cgccatcaga ggcggtactg
     9481 tgggcgtgta aatcatattt cattttattt atccttaaat taagccggca gtcctgaaaa
     9541 attggcgata ggcttcggtt ttgttggcta agctgagtgg ataagccctc gacgtcgaag
     9601 gcaggcgata taaataaagt tctcttgtgg cggaataagg atagaccacc cattcgttgg
     9661 ttttcggagc tttgtgtttt tccggcaata aaccgagcaa cgtatcggtt gccaaaccac
     9721 ctgtggtaaa tagccatttc agtttgggta atttgagcaa cagttcgtcc atatcgatag
     9781 gatcaacaat ttttaaatct ttatctgaag cgtttccttt taaacgaatc gctgtttttg
     9841 ccgaagaaca gagcgcaacg cccttttctc gcaaaaacgc ctcaatccga atcggatcaa
     9901 agcgtttttc ttcaccaact cgaaaatagt tagggtcagc ataaaaaata ttgcccataa
     9961 ttcgccacat atcattttga aaattcgggt aatgaaattc catacaacgc ttttccgatg
    10021 taggtggaaa agtgccgacc attaccaccg ttgcttgttc aggtaaaata gggtcaaacg
    10081 ggtgctgttc tagcggtaaa ttatccattg tcatttaatc aactctttta acatcttgag
    10141 cttttcggtt ttatctaggg gctgttccgc tactctgtta agctcgctca gcaatttttc
    10201 atcttttgca acattttgac gataaatgac cgcttgttgt tgcgaaatct gctcggcgta
    10261 agcggtcaaa ttttctaata attttacctt ttccgggttg tcgtgatgtt gttggaacaa
    10321 gtcgtcaatc gattgggcga actgcgctgc ttttgcctca atttgctggt tgcaatgttt
    10381 ctgccatttg atctgctcgg ctcgactgag tgtttgtgga tagtgccttg ctcggtaatg
    10441 gaatagtaac gcctctactc gagggtcttc aaatttcaag ccgtggttgg ctaaatcttc
    10501 aggcggtagc gtgcgtaaaa tcgccatatt atttttatcc gcaggcgaga aaaagccgtc
    10561 atacaaagtg gtttctacat tggtcgaagg ctcaaacgtg cgttcttcat taaaaatttc
    10621 aatcactttt tcccgaatca gcgatttctg tgcttttaaa gttttcaaat tttccaaaca
    10681 taaagctctg tctatgccta aacgttctgc attttcaggc agtaaggttt ttgccggtgc
    10741 taaaatcggg catttattaa tatgcactaa ttttaacggc acaggtaact cattttcggc
    10801 taattcatct cgtttggtgt ataaacgctg acgtaaaacc tctgcatttt cagtcaataa
    10861 gccattcata tcgcccgata aatcacacac aattacggca ttttgattag ttgagtgcca
    10921 agccagtggc acaacccaag cggtatttcc tcgatagttg cccagcatac cggaaacgtg
    10981 cacaagcggt gtcatttcac cagtgtcaat taacttctct acttcttttt tactgcgtaa
    11041 attaaagaaa aaatcgaaca actttggctg tttttgctta attaattttg ccattgcgat
    11101 ggtggcatac acatcagaca tcgcatcgtg tgcattttca tgggcaatgc cattggcttt
    11161 ggtgaggttt tccaatttaa aacttggcat accggtttca tctgttggcc attcaatacc
    11221 atcaggtctt aacgcataac aggcacggac taaatctaac aagtcccaac gagaatttcc
    11281 gtttttccag ctatattcgt aaggatcgta aaaattgcgg aagaaggtat aacgggtcat
    11341 ctcatcatca taacgaatat tgttataacc gatagtacaa gtattcgctc ggctaaattc
    11401 agcgtgaatt cgggctgcaa attccggctc agatacacct tcagcgttac attgttgcgg
    11461 tgtaatcccg gtaaccatca ccgcttcggg gctgggtaaa taatccggcg tttgcttaca
    11521 aaaaaacatt accggttcac cgataatatt aaaatccgca tcggttcgaa taccggcaaa
    11581 ttgtgccgga cgatctaacg ccggactcac gccgaaactt tcataatcgt aaaagaaaaa
    11641 agagaattta tcgctcattc gttcaatttg ttagaaaaaa ttgggctgat tgtagcacag
    11701 taaggcagaa aggagggaat acaagcggtc aatttccgtt agaaatttgc aaatgctaaa
    11761 ataaaaaaag cccctaatca aaaaagcgaa aaggggcgga ggtcttacct aatgataata
    11821 ttatttttat aaatcaatta gttaagatgt ttttgtcgta aaaatatcga gattattaaa
    11881 aacacctaca ataaagcaat acctcagcac tcgaagagta agaccgtctt tgcgggatag
    11941 gggtaacata aatt
//
LOCUS       DANLYI010000059        11855 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.059, whole genome shotgun sequence.
ACCESSION   DANLYI010000059 DANLYI010000000
VERSION     DANLYI010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 11855)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11855
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.059"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            complement(6..82)
                     /locus_tag="PXD81_001183"
     tRNA            complement(6..82)
                     /locus_tag="PXD81_001183"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(46..48),aa:Pro,seq:tgg)
     gene            complement(107..182)
                     /locus_tag="PXD81_001184"
     tRNA            complement(107..182)
                     /locus_tag="PXD81_001184"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(147..149),aa:His,seq:gtg)
     gene            complement(212..288)
                     /locus_tag="PXD81_001185"
     tRNA            complement(212..288)
                     /locus_tag="PXD81_001185"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(252..254),aa:Arg,seq:ccg)
     gene            complement(447..1169)
                     /gene="rffM"
                     /locus_tag="PXD81_001186"
     CDS             complement(447..1169)
                     /gene="rffM"
                     /locus_tag="PXD81_001186"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HDL5537215.1"
                     /translation="MFDKVIIRGIALQAVKNREIFANWLMNEKRIQFGRLVAINAEKV
                     ILSEKNPLLRRVIDEAEFNYADGISVVQSIRKKYPNYKIERIAGADLWETLMQRAGAL
                     NVPVFLVGSSADTLTKVEAKLAQWKVNIVGRQNGYFKAEQEQDVIEQIKASGAKFISV
                     AMGTPKQELFIQQLQQQYPQALYMGVGGTYDVFAGKVKRAPKIWQNLGLEWLYRLLKQ
                     PTRWQRQLNLLKYAYYYLTNQL"
     gene            complement(1159..2430)
                     /locus_tag="PXD81_001187"
     CDS             complement(1159..2430)
                     /locus_tag="PXD81_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WzyE family oligosaccharide polymerase"
                     /protein_id="HDL5537216.1"
                     /translation="MNNLIPLGGFYLVSVGLFFSLLYQLYQKSGISFHLLFSLLYFVT
                     FYAGFPFSLIMAFGFDYPLPELKTQWQVFTAAGVGYLIYYVVYSRLWSAVSQPTALVA
                     NFQAKTTACILAAVAVVALLYFLYLNGFLLFKLEKYSQLFAKDRVILSALKRFFYFLL
                     PALLIFCFLYKNKTAWWALLIVGGAFGILSYVAVGGTWANLALAVGFFLLIGLQQGYL
                     SLSKVIAVSLIGVVAMFFLALARYKLTLSGSEMIYTFLYLSRDTFSPWENLARVFSNE
                     VEYQGLMPIVRDFYVYIPQSLWQERPEIVWNTANYFTKVILGNQSGLAISPTLLGSFY
                     IMGGFSVMALGMGIVAAMIKALDNLFAYAKACNSALLQAYCFGNLFNLIVLVREGIDA
                     FVSRFCFFTLIFMACYLLAKILSMGKNNNVR"
     gene            complement(2423..3493)
                     /locus_tag="PXD81_001188"
     CDS             complement(2423..3493)
                     /locus_tag="PXD81_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HDL5537217.1"
                     /translation="MLPIYHILGSEIEHHNQQVLTFFQQELLPLLPDSEHFFYVVGKP
                     SLQTAFPHLRIKQFSSQKAIANALIAKKRQNPTACFLLHGQFNPWIWLAIALGRLPTT
                     NLIWHIWGADLYEMATSWKFKLFYPFRRVAQQKILQVWATRGDLDHLWRNMRTRSEQD
                     HLIYFPTKLGDQIKISPTQNDRLTILLGNSGDPANELIQALSEIHQRLGNNVRIIIPM
                     GYPENNESYIRQVEAYSKRLFLAENLQILRKKLSFEEYVTLLSHCDLGYFNFKRQQGI
                     GTISLLISHNVPCVLHSANPFGLDLQAEKVPYLLAENLTASQIQTTKQQLKSIDKNRI
                     AFFAPGYTKLWQQRLIELMQNE"
     gene            3680..5101
                     /gene="glnA"
                     /locus_tag="PXD81_001189"
     CDS             3680..5101
                     /gene="glnA"
                     /locus_tag="PXD81_001189"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011298219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HDL5537218.1"
                     /translation="MSNAIANVSKLIQDNDIKFVLLKFTDIKGKEHGVSLPVSLVAND
                     LEDLFEEGKMFDGSSVEGWKAINKADMLLMPIAETAVVDPFARIPTLSIRCSIYDPNT
                     MQSYDRDPRSIATRAENYLKSTGIADSVMFGPEPEFFLFDDVRFSTEMNNVSYRIDDV
                     EAAWNTNRKFEEGNKAYRPLKKGGYCAVAPIDTAHDIRSEMCLLMEEMGLVIEAHHHE
                     VATAGQNEIATRFNSLTLKADETQIYKYIVQNVAVEHGKTACFMPKPFAGDNGSGMHC
                     NMSLSKDGKNVFMGDKYAGLSETALYYIGGIIKHAKALNAFTNPTTNSYKRLVPGFEA
                     PVLLAYSASNRSASIRIPAVTSPKATRVEARFPDPMANPYLCFAALLMAGLDGVINKI
                     HPGDAMDKNLYDLPPEELKDIPTVASSLESALDALSNDFEFLTQGGVFTKDFIDAYIG
                     IRRKEVERVNMTPHPVEFELYYS"
     gene            complement(5145..6288)
                     /gene="rffA"
                     /locus_tag="PXD81_001190"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /pseudo
     CDS             complement(5145..6288)
                     /gene="rffA"
                     /locus_tag="PXD81_001190"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251256.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
     gene            complement(6275..6919)
                     /gene="rffC"
                     /locus_tag="PXD81_001191"
                     /gene_synonym="wecD"
     CDS             complement(6275..6919)
                     /gene="rffC"
                     /locus_tag="PXD81_001191"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HDL5537219.1"
                     /translation="MKPDQWLSDFFGREIVQVKVAADDFRQIARLQKQGFVLVEGELE
                     FELALATFSENMTACRPADMADLPDLNLLFGSAFPDSRFRSPYFSREENQRFYRTWIE
                     NAVKGKFDDIGLVQCNADGQIQGAVTVRLLAQQQAKIGLLAVKPDCRQQGIGSELLNA
                     AIAWAKQQNAKKLMITTQLSNQKAIRFYQAFGASIKAVYYWFYPALEKYDDEQS"
     gene            complement(6916..8163)
                     /gene="wecC"
                     /locus_tag="PXD81_001192"
     CDS             complement(6916..8163)
                     /gene="wecC"
                     /locus_tag="PXD81_001192"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HDL5537220.1"
                     /translation="MMSKTEFNTICILGLGYIGLPTSVVFANAGKQVIGVDINPQVVA
                     SLNQGNIHIIEPDLQTAFTQAVKKGNFFATAKPQPADAFIIAVPTPLCGKQPDLTYIQ
                     NAAAMIAPCLRKGNLVVLESTSPVGTTENLAEWLSQARPDLAFPADVSIAYCPERVLP
                     GNIMTELVENDRIIGGLSEQCSQQAVDLYQIFAKGRCVTTTARTAEMCKLVENSFRDV
                     NIAFANEISMICDALKINVWELIELANLHPRVNILQPGAGVGGHCLAVDPWFIVSTSP
                     QQARLIRTAREVNDSKPQWVIEQVKTSVADCAMEQDCKPSELTIACLGLAFKPNIDDL
                     RESPALAITRQLAAWHQGSLLAVEPNIQQLAGEKITLVDLNTALSQADILVLLVDHTS
                     FKQISREQIRQRHIIDCRGVWVR"
     gene            complement(8204..9016)
                     /locus_tag="PXD81_001193"
     CDS             complement(8204..9016)
                     /locus_tag="PXD81_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS chain length-determining protein"
                     /protein_id="HDL5537221.1"
                     /translation="MKTLFYRLLVLILFAAIGAGAGYGLSYTQPEKWKVTAQFEQPTI
                     PDLGNYYSLLSTYHFLNSNSQEANSQTAQQQASEQVYAEFKRNLTSADLLQAYLLQTE
                     AVKLKAQVAKKSPAVVAAELVEQFRFEKDMATSADNVSLIAENAEEAQKLLADFIPLV
                     NTQARDKLNADLIAKWKVLFQQIKTASELNINGKQGSQDWAAKLQMMRSVQPLDNQLT
                     AYRLVKSPSVPLKSESPDPPILADDWGIKWLTAGLYAQFCGESESSTCGTLI"
     gene            complement(9027..10091)
                     /gene="wecA"
                     /locus_tag="PXD81_001194"
     CDS             complement(9027..10091)
                     /gene="wecA"
                     /locus_tag="PXD81_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HDL5537222.1"
                     /translation="MWLTFLTVFIVSFLALIVMRPVAEFVGLVDKPNFRKRHQGLIPL
                     IGGIALFIGNLTFYFMQWQDMRLPELYLTAVTILLIIGVLDDRFDISPLLRAGIQAAL
                     AGAMIYSGLSLSSLGQIIAPFSIELGWLGIVFTVFITIGVINAFNMVDGIDGLLAGLS
                     SVSFAGLGTLMFINGEPSLGYWCFALIFVLLPYAMFNLSLFGAKWKVFMGDSGSMLIG
                     FTIIWILLLSTQGQGSPISPITGLWLIAVPLIDMVAVIIRRLKKGKSPFKPDRLHLHH
                     LMMRAGLTSRQALLVITLWAGFCSTIGVLGEVYYWNQWVMMLMFIGLFFLYAYSITHA
                     WRVTRFVRRLKRRAKRSKQL"
     gene            10233..11513
                     /gene="hemL"
                     /locus_tag="PXD81_001195"
     CDS             10233..11513
                     /gene="hemL"
                     /locus_tag="PXD81_001195"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005721785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HDL5537223.1"
                     /translation="MSISEQLFEQAQKVIPGGVNSPVRAFKGVGGTPVFIEKAEGAYI
                     TDSDGKQYIDYVGSWGPMVLGHNHPAIIDAVLKAVPKGLSFGAPTAAEITLAELVCKL
                     VPSIEMVRMVSSGTEATMSAIRLARGYTNRDKIIKFEGCYHGHSDSLLVKAGSGALTL
                     GQPSGPGVPADFAKHTLTCTYNDLDSVKAAFEQYPNEIACLIVEPVAGNMNCIPPQEG
                     FLQGLRDLCTQYGAVFIIDEVMTGFRVALGGAQSYYGVTPDLTTLGKIIGGGMPVGAF
                     GGKKEIMEYIAPTGPVYQAGTLSGNPIAMAAGLACLTELSKAGNETILAEKTKTLAEG
                     FKALADKHNVPLTVQYVGGMFGLFFTEQKAVNNFQEVMKCDAAKFNRFFHLMLEQGVY
                     LAPSAFEAGFMSLAHSDEDIARTLEAADKAFAQL"
BASE COUNT         3501 a         2761 c         2408 g         3185 t
ORIGIN      
        1 taaattggtc ggcgagatag gatttgaacc tacgacccac tggtcccaaa ccagttgcgc
       61 taccaagctg cgctactcgc cgtctgatac tcacaagagt attgaatggg gtggctaatg
      121 ggattcgaac ccacgacaac tggaatcaca atccagggct ctaccaactg agctatagcc
      181 accattgtgt tgatagtgcc gcattgacat ctggcgcgct cgacaagatt cgaacttgcg
      241 acctttggct ccggaggcca acgctctatc caactgagct acgaacgctt cgatttacat
      301 caattcaact taatgcgtcg caacggaggc gtatattata gatttcactt tcccttgtct
      361 agcacttttt aaaaaaaatg taaaaaaatg tattgtttgg ttttatttta ttcaaaacgt
      421 ttaataaata tcaaaaaatg cgttttttag agctgatttg tcagatagta ataggcatat
      481 tttagtaaat ttaattgtct ttgccagcgg gttggctgtt tcaatagtcg ataaagccat
      541 tccagcccca aattttgcca aattttaggt gctcgtttca ctttaccggc aaacacatcg
      601 taagtcccgc caacacccat atataaggct tgtggatatt gttgttgtaa ctgttggata
      661 aaaagttcct gttttggagt tcccattgcc acactaataa acttcgcgcc acttgcttta
      721 atttgctcta taacgtcctg ttcttgctcc gccttaaaat agccattctg cctaccgaca
      781 atattcacct tccattgtgc caattttgct tccaccttcg ttagggtatc cgccgagctg
      841 ccgaccaaaa acaccggcac atttaacgct ccggctcttt gcattaaggt ttcccataaa
      901 tcggctccgg caattcgctc tattttatag tttggatatt tttttcgaat cgattgaaca
      961 acactgatgc cgtctgcata gttgaactcg gcttcatcta tcactcggcg taacaacgga
     1021 tttttttccg acagaatcac tttctcagca ttaattgcca ctaatcttcc gaactgaatc
     1081 cgcttttcat tcatcagcca atttgcaaaa atttcccgat ttttgaccgc ttgtaacgca
     1141 atgccccgaa tgatcacttt atcgaacatt attatttttc cccattgaaa ggattttcgc
     1201 cagcaaatag caagccataa atatcagcgt gaaaaagcaa aaacgggaaa caaatgcatc
     1261 gattccctct cgcactaata cgatcaaatt gaataaattg ccgaagcagt aggcttgcaa
     1321 caaggcggaa ttgcacgctt tggcgtaagc gaataggtta tctaaggctt taatcatggc
     1381 agctactatc cccattccca acgccataac gctaaatccg cccataatat agaaagagcc
     1441 gagtaaggta ggcgaaatag ctaaccctga ttggttgcct aaaatcactt tggtaaaata
     1501 attcgccgta ttccagacga tctcagggcg ttcttgccat aaagattgtg ggatatatac
     1561 atagaaatcc cgcacaatcg gcattaaacc ttgatattcc acctcattag aaaatacacg
     1621 agccaaattt tcccaaggcg aaaaagtatc tcggcttaaa tataagaagg tgtaaatcat
     1681 ctcgctaccg gaaagggtaa gtttgtaacg ggctagagcg aggaagaaca tcgccaccac
     1741 gccgattagg cttacggcaa tcacctttga taacgacaga tagccttgct gcaatccaat
     1801 caataagaaa aagccaaccg ctagtgctaa atttgcccat gtgccgccca ctgccacata
     1861 gcttaatatg ccgaatgccc caccaacaat cagtaacgcc caccacgctg ttttattctt
     1921 atataggaag caaaaaatta acaacgccgg caataaaaag tagaaaaatc gttttaatgc
     1981 cgaaagtatg accctatctt tggcaaacag ttggctatat ttttcgagtt tgaaaagcag
     2041 aaaaccgttt aaataaagga aatatagcag ggcaacaact gccacagcgg caaggataca
     2101 agcggtcgtt tttgcctgaa aatttgcaac aagagcagtt ggttgcgaca cagcactcca
     2161 caagcgtgaa tagacaacgt aataaatcaa atagccaaca cctgctgccg taaaaacttg
     2221 ccattgtgtt tttagctccg gcagcgggta atcaaagcca aatgccatta ttaacgaaaa
     2281 cggaaaaccg gcgtaaaaag taacaaaata gagcaaacta aacagtaaat ggaaagaaat
     2341 ccccgatttc tgataaagtt gataaagtaa gctgaaaaat agcccgacag aaacaagata
     2401 aaatccgcca agtggtatta aattattcat tttgcattaa ttcgattaat cgctgttgcc
     2461 atagtttagt gtagcccgga gcaaaaaacg caatgcggtt tttatctatg ctctttagtt
     2521 gttgtttggt tgtttgaatc tgtgaagcag tcagattttc cgccaacaaa taaggcactt
     2581 tttctgcctg tagatccaac ccaaacggat tggctgaatg taacacacaa ggtacattat
     2641 ggctaatcaa aaggcttatc gtgccaatgc cttgctggcg tttaaaattg aaatagccga
     2701 gatcacaatg gctcaataac gtaacatact cttcaaagct cagcttcttc cgcaaaattt
     2761 gcaaattttc cgccaaaaat aaccgcttgc tataggcttc cacctgccga atatagcttt
     2821 cattattttc cgggtagccc attggtatga taatccgcac attgttccct agtcgttgat
     2881 gaatctcgct taaggcttga ataagctcat tcgctggatc gcctgaattg ccgagcaata
     2941 tcgtcaagcg atcattttgt gtggggctaa tttttatttg atcacctaat ttcgtgggga
     3001 aatagattaa atgatcctgt tctgatcttg tacgcatatt tcgccacaaa tgatccagat
     3061 cgcctcttgt tgcccaaacc tgtaagattt tttgttgggc aacacgccga aacgggtaaa
     3121 acagcttaaa tttccacgaa gttgccattt catataaatc cgccccccaa atatgccaaa
     3181 tcaggttggt ggtcggcaat ctgcctaagg ctatcgccag ccaaatccaa gggttaaact
     3241 gcccgtgtaa cagaaaacaa gcggtcggat tttgccgttt ttttgcaatt aaggcgttcg
     3301 caatcgcttt ttgtgaagag aactgcttaa tccgcaaatg aggaaaagca gtttgcagtg
     3361 aaggcttgcc taccacataa aaaaaatgtt cggagtctgg caataacggc aaaagctctt
     3421 gctgaaaaaa ggttaaaacc tgttggttat gatgctcaat ctcggagcct agaatatgat
     3481 aaatcggcaa cattacacgc ttttctctcc ccaccataca aacaatatgc aagcggtaag
     3541 attttagcaa aaatttgcaa cattactgca acttctttta gctttgaaac actaaaaaac
     3601 atcacacact tggttaaaaa atgctatgat aaccgagctt ttttattatt catttatccc
     3661 aacgaggact tattttacta tgtcaaacgc aatcgctaat gtatctaaac tgattcaaga
     3721 caatgatatt aaatttgtct tattaaaatt tactgatatt aaaggtaaag aacacggggt
     3781 ttcgctccct gtcagcttag ttgccaacga tttggaagac ttattcgagg aaggcaaaat
     3841 gttcgacggt tcgtccgtag aaggttggaa ggcaattaac aaagcagata tgttgttaat
     3901 gccgattgct gaaaccgctg tagttgaccc tttcgcccgt attcctaccc tttctattcg
     3961 ttgttccatt tacgacccaa acacaatgca gtcttatgac cgtgaccctc gctctattgc
     4021 aacccgtgcc gaaaactact tgaaatccac cggtatcgcc gatagcgtaa tgtttggacc
     4081 tgagcctgaa tttttcttat tcgatgatgt gcgttttagc accgaaatga acaatgtgtc
     4141 ataccgaatt gacgatgtag aagcggcttg gaataccaac cgtaaatttg aagaaggcaa
     4201 caaagcctat cgtccgttga aaaaaggcgg ctactgtgcg gttgcaccga ttgataccgc
     4261 ccacgatatt cgctccgaaa tgtgtttatt gatggaagag atgggtttag tgattgaagc
     4321 tcaccaccac gaagttgcga cagccggaca aaatgagatc gcaacccgat tcaacagcct
     4381 gaccttaaaa gccgatgaaa cccaaatcta taaatacatc gtgcaaaatg ttgccgtaga
     4441 acacggaaaa accgcttgct ttatgccaaa accgttcgcc ggcgacaacg gctcgggaat
     4501 gcactgtaat atgtcattaa gcaaagacgg caaaaatgtg tttatgggcg ataaatacgc
     4561 cggcttatct gaaactgccc tctactatat tggcggtatc attaaacacg caaaagcatt
     4621 aaatgccttt actaacccaa ccacgaactc ttataaacgt ttagtgccgg gttttgaagc
     4681 accggtgtta ttggcatatt ctgcaagtaa ccgctctgcc tctatccgta ttccggctgt
     4741 gaccagtccg aaagcgaccc gtgtagaagc acgcttcccg gatccaatgg caaacccata
     4801 cctttgcttt gccgccctgt taatggctgg cttagacggt gtgatcaaca aaatccaccc
     4861 gggcgatgca atggataaaa acttatacga tttaccgccg gaagagttaa aagatattcc
     4921 aacggttgcc tcgtctttag aatccgctct tgacgcatta agcaatgatt ttgaattctt
     4981 aacacaaggt ggcgtgttca cgaaagactt tattgatgct tatatcggta ttcgtcgcaa
     5041 agaggtggaa cgtgtaaata tgactccgca cccggtagaa tttgaacttt actattcata
     5101 gtaacaacat atagcaaaaa acggctgaaa cactcagccg ttttttattg attgaagaaa
     5161 gcaagcactg cttgaataac acgctcttgc tcctgcttac tcaaattata aaacagtggc
     5221 aatcttacca accgttcgct ttctaaggta gtaaagcgat catcacctgc taatcgacct
     5281 aatttctgcc ctgccggact gctgtgcaac ggcacataat gaaacacggt taagatattc
     5341 tgtgccttca taaaatcaat aaaagcagtt ctctgcttca agtcacttag ctttaagtag
     5401 aacaaatgcc cgttatggcg acaatattcg ccaatgcgag gaagctcgca cttagctgtt
     5461 tgggcaagtg gcaaaaaggc ttgatagtaa ttgtgccaaa gggttaaacg ctgttggttg
     5521 attttatctg cctctataag ctgagcatac aaataagccg cttgaatttc cgacattaaa
     5581 aaacttgaac caatatcctg ccagcgatat ttatctacct caccacgaaa aaattggcta
     5641 cggttagtgc ctttctctct gatgatttcc gctttttcca gcaaatgcct cattaatcac
     5701 gatcgcccca ccttcaccgc cagcggtata gtttttggtt tcgtgaaaac tatagcaacc
     5761 aatatgcccg atggaaccta acgccctacc tttataaaac gaattgactg cctgtgccgc
     5821 atcttctacc acataaagcc cgtatttttc ggctgttgcc attattgaat ccatatcaca
     5881 agcaatgccg gcataatgca ccggcacaat cactttggtt tttggggtaa tggcttgctc
     5941 aattttgcgt tcatcaatat tcattgtatc cggacgaata tccacaaaca caatctttgc
     6001 cccacgcaac gcaaacgcat ttgcagtaga gacaaaagta tagctcggca taatcacttc
     6061 atcgtccggc tgaatatcca gcagtattgc cgccatctct aacgcagccg tgcaagaagg
     6121 ggtaagcaac actttgctac gggccaggtt ttcaagccac tgctcacata agcgggtgaa
     6181 tttaccaccg cccgaaagct gccctgttgc catcgcctgt tctatatagg cttgctccgc
     6241 ccccacaacc gggggttgat taaacggaat aaaatcagga ttgttcatca tcgtattttt
     6301 ccaaagcagg gtaaaaccaa taatacaccg ccttaatgct tgcaccaaac gcttgataaa
     6361 aacgaatcgc tttctggtta ctcagctgag tggtaatcat cagttttttc gcattttgct
     6421 gtttcgccca cgctatcgca gcatttaaca attccgagcc aataccttgt tgtcggcaat
     6481 caggctttac cgccaataat ccgattttcg cttgctgttg ggcaagtaag cgaaccgtta
     6541 ccgccccttg aatttgcccg tcagcattgc attgaaccaa gccaatatca tcaaacttgc
     6601 cttttaccgc attttcaatc caagtccggt aaaagcgttg attctcttcc cttgaaaaat
     6661 agggcgaacg aaaccggcta tcgggaaatg ccgaaccaaa caataagttc aagtcgggca
     6721 aatctgccat atccgccggg cgacaagcgg tcatattttc agaaaatgtt gcaagtgcca
     6781 gctcaaattc caattctccc tccacaagta caaaaccttg tttttgcaac ctagcgattt
     6841 gcctaaaatc atcagccgct acttttactt gcacaatttc tctaccgaaa aaatcggaca
     6901 gccattgatc cggcttcatc ttacccacac tcctcgacaa tcaataatat gccgttgcct
     6961 gatctgttcc cttgaaattt gcttaaatga agtgtgatcc actaacaaga ctaaaatgtc
     7021 tgcctgcgaa agtgcggtgt tgagatcgac taaggtgatt ttttcgccag ccagctgttg
     7081 aatatttggc tcaaccgcca acaggctacc ctgatgccaa gcggcaagtt gccgggtaat
     7141 cgctaatgcc gggctttccc gcaaatcatc aatattcggc ttgaaagcta gccctaaaca
     7201 agcgatggtt agctcacttg gcttacaatc ttgctccatc gcacagtctg cgaccgatgt
     7261 tttcacttgc tcgattaccc attgcggttt gctgtcattc acttctcgtg ccgtgcgaat
     7321 aaggcgagcc tgttggggcg atgtggaaac gataaaccac gggtcaaccg ccaaacaatg
     7381 tccgcccacc cctgctccgg gctggagaat atttactctc gggtgaagat ttgccaattc
     7441 aattagctcc cacacattga tcttcaacgc atcacaaatc attgaaattt cattggcaaa
     7501 ggcaatgttc acatcacgaa agctgttttc caccaacttg cacatctccg ctgtgcgagc
     7561 tgtggtggtg acacaacgtc cttttgcaaa aatttggtat aaatcgaccg cttgttgact
     7621 gcattgttcg cttaatccgc cgataatgcg gtcattttca accaattccg tcattatatt
     7681 acccggcaaa acacgttcag ggcagtaggc aatagacaca tcggctggaa aggctaaatc
     7741 agggcgagct tggcttagcc attcggctaa attttctgtt gtaccgacag gcgaagtgga
     7801 ctccagcacc accaaattcc ctttacgcaa acaaggggca atcatagcgg cggcattttg
     7861 aatataagtg agatcgggct gcttcccgca aagtggggtt ggcacggcga tgataaaagc
     7921 atcggccggt tgcggtttgg cagttgcaaa aaaattacct tttttgaccg cttgtgtgaa
     7981 ggcagtttgt aaatccggct cgataatatg aatgttgcct tgattaaggc ttgccaccac
     8041 ttgcggatta atatccaccc caattacttg cttgccggca ttggcaaaaa cgactgaggt
     8101 cggcaagccg atatagccta accccagaat acaaatagtg ttaaattcag ttttactcat
     8161 catcagttac aaagagaaat ggcacactaa attgtgtgcc attttagatt aaagttccgc
     8221 aggttgagct ttcgctttca ccgcaaaact gagcataaag cccagcagta agccacttaa
     8281 tgccccaatc atcagccaaa ataggcggat caggcgattc tgatttaagt ggaacgctag
     8341 gcgatttaac caagcgataa gctgtaagtt ggttgtctaa cggctgaaca gaacgcatca
     8401 tttgcagttt agctgcccaa tcttgtgagc cttgcttacc gttaatattc aactccgaag
     8461 cagttttaat ttgctggaac aagactttcc atttcgcaat taaatcggcg tttaatttat
     8521 ctcgagcttg ggtgtttacc aacggaataa aatcggctaa taatttttga gcctcttccg
     8581 cattttctgc aattaagctg acattatctg ccgaagttgc catatctttt tcaaaacgga
     8641 attgttcaac taattcagcc gccaccaccg ccggtgattt ctttgcaacc tgagctttta
     8701 atttcaccgc ttctgtttgt agcaaatagg cttgaagcaa gtcggcggac gtgaggttgc
     8761 gtttaaattc cgcataaacc tgctcacttg cctgttgctg tgccgtttgg ctattcgctt
     8821 cttggctatt actattcaaa aaatggtagg tggaaagtaa cgagtaataa ttacctaaat
     8881 cgggaatagt cggttgctca aattgagcgg tcactttcca tttttcaggt tgagtataac
     8941 tcagcccata accggcacct gccccgatag cagcaaataa aattagcact aaaagtcgat
     9001 aaaataaggt tttcacaaca tatcctttaa agttgtttgc tacgttttgc acggcgtttt
     9061 agacggcgaa caaaacgggt tacacgccaa gcgtgggtaa tggaataggc gtataggaaa
     9121 aacagcccga taaacattag catcatcacc cactgattcc aataatacac ctcgcctaat
     9181 accccaatgg tggaacaaaa tcccgcccag agggtaatca ccaataatgc ttggcgagag
     9241 gtaagccctg ctcgcatcat taaatggtgt aggtgcaagc ggtctggttt gaacggactt
     9301 ttaccttttt tcaaacggcg gataatcacc gcaaccatat caattaacgg cacggcaatc
     9361 agccacaaac cggtaatcgg gctaattggc gagccttgcc cttgggtgct taacagcaga
     9421 atccaaataa tggtaaagcc gatcagcata ctgcctgagt cgcccataaa cactttccat
     9481 ttcgccccaa acaggctcaa attgaacatt gcatacggca acaacacaaa gataagggca
     9541 aagcaccaat agcccaaact tggttcgcca ttaatgaaca ttaaagtgcc aagaccggca
     9601 aaactcacgc tggaaagccc tgctaataag ccatcaatcc catctaccat attaaacgca
     9661 ttaataacgc caatggtgat aaacacggta aagacaatgc cgagccaacc taattctata
     9721 ctaaaggggg caataatttg ccctaaactg ctgagcgaca agccactgta aatcatcgcc
     9781 ccagctagtg ctgcttgaat cccggcacgc agtaatgggc tgatgtcaaa tcggtcatct
     9841 aacacgccga taatcagcaa aatagttacc gctgtcagat acagttccgg cagccgcata
     9901 tcttgccact gcataaaata gaaagtgaga ttgccgataa ataatgcaat accaccgatt
     9961 aacggaatca acccctgatg gcgtttacgg aagttgggct tatctaccaa accaacaaat
    10021 tccgccaccg gacgcatcac aatcagggct aaaaaagaaa caataaatac ggttaaaaaa
    10081 gtaagccaca tacagggatt gcctcaaaaa atgagtcata aaataggaat gcagaatagc
    10141 ataaggcaac aaaatcgtaa attttttagc gaaaacaagg gcttttcagt acaataacag
    10201 gcaaattttt acatcaaaag aaaggaattg atatgtctat ttcagaacaa ctgtttgaac
    10261 aagcccaaaa agtgattccg ggcggtgtga actcccctgt gcgtgcattt aaaggcgtgg
    10321 gcggcacacc ggtttttatt gaaaaagccg aaggtgctta tattaccgac agcgacggca
    10381 aacaatatat cgactatgtc ggctcttggg ggccaatggt actcgggcat aaccacccgg
    10441 cgattattga tgcggtgcta aaagccgtgc caaaaggctt aagtttcggt gccccaacgg
    10501 cagcagaaat cacacttgcc gaattagtgt gcaaattagt accttctatt gaaatggtga
    10561 gaatggtgag cagcggaaca gaggcaacaa tgtctgccat tcgtttggct cgtggctata
    10621 ccaaccgaga taaaatcatc aaatttgaag gctgctacca cgggcattcc gattctctgt
    10681 tagtaaaagc cggttcaggt gctttaaccc tcggtcagcc aagcggcccg ggcgtacccg
    10741 ctgatttcgc caagcatacg cttacttgca cctataatga tttagattca gtaaaagcag
    10801 catttgagca atacccaaat gagatcgctt gtttaattgt tgagcctgtt gccggcaata
    10861 tgaactgcat tccgccacaa gaaggctttt tacaaggctt acgtgatctt tgcacacaat
    10921 atggtgcggt atttattatt gatgaagtaa tgaccggctt ccgtgtcgca ctcggtgggg
    10981 ctcaatctta ctatggggta acaccggatt taaccacact cggcaaaatt atcggcggcg
    11041 gaatgccggt gggggcattt ggtggtaaaa aagagattat ggaatacatc gctccaactg
    11101 gcccggttta ccaagccggc acgctctcag gcaacccgat tgcaatggct gcaggtttgg
    11161 catgcttaac tgaattatca aaagcaggaa atgaaaccat tttagcggaa aaaaccaaaa
    11221 ccctcgctga aggctttaaa gctcttgcag ataagcacaa tgtgccatta acggtacaat
    11281 atgtcggcgg aatgttcggc ttattcttca ccgaacaaaa agcagtaaac aacttccaag
    11341 aagtaatgaa atgtgatgcc gccaaattca accgtttctt ccacttaatg ctggaacaag
    11401 gcgtgtattt agccccatcg gcattcgagg cagggtttat gtctttagct cacagtgatg
    11461 aagatattgc tcgtacgcta gaggctgcgg ataaggcatt tgctcagctt tagaagaatg
    11521 acaagcggtt gggtttactc aataatttgc aagtttttga gggaatttga ccgcttgtag
    11581 tgcaaatttc tcttacgata gcaattatga tggtgttcgt ctctgacgag tgcttataca
    11641 aactttcatt atgcttaaac aagcacccgt ctggagacgg gcgctatcga gggaatagga
    11701 ttgataaagc aatatatggg cttccaacac ttgcaggcaa ggacaagtct tacactttct
    11761 aatattacaa atatatttct ttctttaatc tataccataa aggtttaagt tggttcttta
    11821 taactttgga atccatagat gaataagctc tcttt
//
LOCUS       DANLYI010000060        11666 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.060, whole genome shotgun sequence.
ACCESSION   DANLYI010000060 DANLYI010000000
VERSION     DANLYI010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 11666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11666
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.060"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            233..961
                     /gene="ttrB"
                     /locus_tag="PXD81_001196"
     CDS             233..961
                     /gene="ttrB"
                     /locus_tag="PXD81_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetrathionate reductase subunit TtrB"
                     /protein_id="HDL5537224.1"
                     /translation="MDLSKRSFLKELSALTVGASFVPLQSAQAFMPAWREGDENKRYA
                     MLIDLRKCIGCQACTVSCSVENATPLHSFRTTVRQYEITNGTQVVNNVVLPRLCNHCD
                     QPPCVPVCPVQATFQRKDGVVVINNEQCIGYGYCVQACPYDARFINEETKTADKCTFC
                     THRLEVGLLPACVESCVGGARVIGDLNDPKSQISQLYQAHKDDLKVLKPEAGTVPHVF
                     YVGLDDDAFVSKIDGQPMLWTGEA"
     gene            963..1994
                     /gene="nrfD"
                     /locus_tag="PXD81_001197"
     CDS             963..1994
                     /gene="nrfD"
                     /locus_tag="PXD81_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysulfide reductase NrfD"
                     /protein_id="HDL5537225.1"
                     /translation="MIREVLVEPQHIVWLPWIVHYFFFVGVAATAVFTAVLFAKKSLK
                     PTACELAAVTVALIGSIVAPVALTADLHQPSRILHFYTDFAWRSPMAWGAIILPLFSV
                     AVAGYFVLALAHHTRPNLPKWLAWLQLPILQNQGLLWAFRLFATLTAIGIIGYTVLET
                     YQTGTRTLWHSAWLLPIMLFSAWAVALGLTQAVCKMLFHTQTNTGLWTKLCLILTALS
                     LVGLAFSSPQATQDFAQLFNGSITAYLVGIFWLIALICNFSAKNHRLQWLGVLALIAF
                     GWLLRWVLVIQVQTIAKTNALQNPYHFDWTAVDGGLGIVSIFGLTVLVSIVVWQLILI
                     ICGGRKGEQ"
     gene            1984..5079
                     /gene="ttrA"
                     /locus_tag="PXD81_001198"
     CDS             1984..5079
                     /gene="ttrA"
                     /locus_tag="PXD81_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetrathionate reductase subunit TtrA"
                     /protein_id="HDL5537226.1"
                     /translation="MNNKRRNLLKGGLALGGAAAFGAGYSPKVSEIAKGVMHGTSGEK
                     TRDAIHGNSLTPEYQVKDGKLLSNPEQIVCNTQCMGCWTQCGIRARVDLNTNQVIRIS
                     GNPYHPLSSDKTLPFGMPIAKAELLLAGESGIENRSTACARGAAFLEGVNSPYRITQP
                     LKRVGKRGEGKWQTISFEQLVEEIINGGNLFGEGEVEGLKTIRNLVDPINPKQPDLGM
                     KANQLLVTFAGPEGRQPILQRFAQKSFGTINFSNHGAYCGAAYRAGSGAFMKDFDNQQ
                     HAKPDWDHAEFILFIGTSPAQSGNPFKRQARQLAKRRPDDNFSYVVVSPRLEMTSSLA
                     TQNNHWVPIKPEGDLALVMGMLRWIIENERFNADYLSRPSLAAMENAGHVSYANASHL
                     IICEPNHPKFGQALRISDLQDVQLDPEEKVEENLIVVKDQASGELISAKDCLQAVIFV
                     EQIAKLLDGSEVLVKSSMQLLKEATQEKTLAEYSDICGVPVQTIENLAKKFTSHGHKA
                     NAITHGGTMHSTGFYTSWAILLLNAMVGNLNKKGGMAVAAGKFKDFGDGPRYNLANFP
                     NEVKPKGTNLARSKKAYQNSSEFKQKVTKGENPYPAQGAWYPFTGGQTTEMFTSMVQG
                     YPYFAKAWINHMANPVYGLTAMNPIALEKLKDPKILPLFVSIDAFMNETTALTDYIVP
                     DTHNFESWGFSTAWGGVPQRTSTARHPIVPSKNAKTAEGDTICMENFVIAVAKKMGLP
                     GFGENAIADLEGNRYPLHRTEDYFLRAAANIAFAGEKPAPDASEQDLLLTGVQRIMPK
                     IEQTLKAEERLKVANVYCKGGRFAPHESAWKEENMEFQWKNCLQIWNENVYKAQHHSN
                     GEHYWGCPRYMSPRFADGSTLEQHYPQSEWGFKLISFKSNIMSSITAPLLRLLSIKPE
                     GLVAMNRLDAEEKGIKHGDVVKLSTPTAGLTVQIVVLDGIARGTIGIEHGYGHKQIGA
                     SSYTIDGVEIPANPMIAKGINLNDLGIIDHTKAVKSPWVDWVCGSAVRNGIPAKVEKL
                     A"
     gene            5220..6065
                     /locus_tag="PXD81_001199"
     CDS             5220..6065
                     /locus_tag="PXD81_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDL5537227.1"
                     /translation="MTFVKKTALSLIATALFATSSFAAEQSQYQHIRNATAKIEYAGK
                     TFLVDPYLAEKDRYLPPNAKKRNPTLDMTVSIDEVLNGVDAVIVTHTHHAHWDDVAQQ
                     RVPKNLPIFVQNAGDARLIRSQGFTDVRVVGKDTPFEGVTLTRTRGQHGTDAMYANPK
                     IAEMVGDAMDVVMKAEGKPTVYLVGDTIWNHEIEHSFNTYKPDFVVLNAGCAKLPNFE
                     GSPIMCTEDVSKAHKIAPQAKILTVHMDALPQTSISSDQMRDYVQKEGLNRVTVPKNG
                     DVIKF"
     gene            complement(6178..7575)
                     /locus_tag="PXD81_001200"
     CDS             complement(6178..7575)
                     /locus_tag="PXD81_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HDL5537228.1"
                     /translation="MKLSKLAFSVALALTVTACTTGNMSNDGTLQQAQQTYQSYDEIT
                     KQYQINEQWWQGYTDAELNRVVDLAIKNNLDLAKSGIAVNKALYNANLVGANLVPTFS
                     GSGSVSAAKGLGSASSNNVSTGVSTTAAQAAFNLSYTVDLWRRLADTASAAEWTHKAT
                     IEDLKATRLSIINAVVTTYYQLAYYRDAIRITEESIRHYEQISRILTNKMNAGAIDSL
                     AVDQAQQAVLNARNSVIDLKTNQKIAEQTLRNLLNVQPNTPLNIRYPNIMSVKLQGVD
                     LNVPVSAIANRPDVVGSLQRLQSAFKTLTATEKSWFPTLTIGGSITGSATNIGNVADN
                     PVGNGLIKLDLPFLDWNRVKNNVKISETDYVTARLNYEQKITSALNEIDRYYYSYTQS
                     RSSLKILQDTNAKNKRISSYYKNRYEQGVSEFREWITALNTELNSQLAILNAKYAILT
                     NENAVYQAMAGKYRR"
     gene            complement(7665..8288)
                     /gene="gmk"
                     /locus_tag="PXD81_001201"
     CDS             complement(7665..8288)
                     /gene="gmk"
                     /locus_tag="PXD81_001201"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HDL5537229.1"
                     /translation="MSLGNLYIISAPSGAGKSSLINALLADLPRSEVQLSISHTTRQP
                     RPAEQDGVHYYFTSHDEFKQLIEQGYFLEWAEVFGNYYGTSLPMIERSLEQGIDVFLD
                     IDWQGARQIREKVPNVKTVFILPPSKTELEKRLIGRGQDSAETIAKRMAEAVSEMNHY
                     NEFDYVIINDDFQQALAELKAILIAERLSLGSQQIRHQHLITDLLNN"
     gene            complement(8401..8874)
                     /gene="gpt"
                     /locus_tag="PXD81_001202"
     CDS             complement(8401..8874)
                     /gene="gpt"
                     /locus_tag="PXD81_001202"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HDL5537230.1"
                     /translation="MSEKYTNEKYVVTWDMFHMHARKLAENLLPATQWKGIIAVSRGG
                     LFPAAVLARELGIRHVETVCIASYDHDSQGELNVLHAADTDGEGFIVVDDLVDTGNTA
                     KEIRRMYPKARFVTVFAKPHGAPLVDDYVIDIPQETWIEQPWDLGITFVQPLARR"
     gene            complement(9020..10429)
                     /locus_tag="PXD81_001203"
     CDS             complement(9020..10429)
                     /locus_tag="PXD81_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF560 domain-containing protein"
                     /protein_id="HDL5537231.1"
                     /translation="MKKYPLLFSLFSPVVWAEKVIEHNTDPISFERQPQAVKFEQNVV
                     KNSQKPTAYQPAYSVNQNVEQQINQAILSQDWATLEPLLSRYPQTENFDPLLWHYAQA
                     ALAYAKKDHQTAVFHYRQLLAKPPSLVYPRFDLALILAEDQQFRAAEQEFKAIQAHLP
                     PELAKIARNTQVQIAENEAWQPDFYLQYTQTDNVNNASSSPIVNVNGRIFRKNADSLP
                     QSAKGVRYGLGLSKLHNVKGNHFVGLTLNYSGIYYWDNRDYREQSVSVSPNYSYRTVA
                     YRLTFSPFIEQNWLGSSRYNVQFGATLNGLRYLNSQWLVSGSFTHLQKRYFEELTASR
                     YNSYQHQLSGGVQWQAVKNGRFFANLDGVREIAREKAQSSNKWLARVGAVFQGERWAT
                     QASIGFGKRYFADKHYLFGYKRLDTEYQANLAVWNRTWHWQGIVPKLNFRYQKIDSNI
                     ADFYSRQNKEVFFTLEKLF"
     gene            complement(10625..11651)
                     /locus_tag="PXD81_001204"
                     /pseudo
     CDS             complement(10625..11651)
                     /locus_tag="PXD81_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249101.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="colicin transporter"
BASE COUNT         3085 a         2747 c         2650 g         3184 t
ORIGIN      
        1 cgatacctag taccccgagt gatattaatg cgtataaaaa ctttagatct aaactaaacc
       61 atcttattgc tttttaatta gtatcaatgc cctattatct acttgtatag ggcatttccc
      121 ttcatccttg acccaaatca actttcccct tgatatgtgg aaaaccacaa taccttgccg
      181 ttccctctcc gccttagaat acctttgtta tttttaaatt tgtgggggaa atatggattt
      241 aagcaaacgt tcctttttga aagagctttc cgccttgacg gtgggggcga gttttgtgcc
      301 gttgcaatcg gcccaggcgt ttatgccggc ttggcgtgag ggcgatgaga acaaacgcta
      361 tgcgatgctg attgatttgc gtaaatgtat cggctgtcaa gcgtgtaccg tgagctgttc
      421 ggtggagaac gccacgccgt tgcacagttt ccgcaccacg gtgcgtcaat atgaaatcac
      481 caacggcacg caggtggtga acaatgtggt gttgcctcgt ttgtgtaacc attgcgatca
      541 accgccgtgt gtgccggtct gcccggtgca ggcaaccttt caacgtaaag acggtgtggt
      601 ggtgattaac aatgagcagt gcatcggcta tggttactgc gtgcaagcct gcccgtatga
      661 tgcccgtttt atcaatgaag agaccaaaac cgccgataaa tgcaccttct gcactcaccg
      721 tttggaggta ggtctgctgc cggcgtgcgt ggaaagttgc gtgggcgggg caagggtgat
      781 tggcgatttg aacgacccga aaagtcaaat ttcccaactt tatcaagccc acaaagatga
      841 tttgaaagtc ctgaaaccgg aggccggcac cgtgccacac gttttctatg tggggctaga
      901 cgatgatgcc tttgtgagca aaattgacgg gcaaccaatg ctctggacgg gggaggcgta
      961 aaatgattcg tgaagtatta gtggaaccgc aacatatcgt ctggttgccg tggattgtgc
     1021 attatttctt cttcgtaggc gtggcggcaa cggcagtgtt taccgccgtg ctgtttgcaa
     1081 aaaaatcact gaaaccgacc gcttgcgagc tggcggcggt gacggtagcc ttgattggct
     1141 cgattgtcgc ccctgtggca ttgactgccg acctgcacca accgagccgt attctgcatt
     1201 tctacaccga ttttgcgtgg cgatctccaa tggcgtgggg agcgattatc ctgcccctgt
     1261 tcagcgtggc ggtagcgggt tattttgtgc tggcattggc tcaccatacc cggccgaacc
     1321 tgccgaaatg gttagcgtgg ctacagttgc cgatactgca aaatcaaggc ttgctttggg
     1381 cattccgcct gtttgccaca ctcaccgcta tcggcattat cggctacacc gtgcttgaaa
     1441 cctaccaaac cggcacaaga accttatggc acagtgcgtg gctgctgccg attatgctct
     1501 tttctgcgtg ggcggtggca ttggggctta cccaagcggt ctgcaaaatg ctgttccaca
     1561 cccaaaccaa cacggggcta tggacaaaac tctgcctgat tttaaccgca ctttcgcttg
     1621 tcggactagc attcagtagt ccacaagcca cacaagattt tgcccagctc ttcaatggct
     1681 caatcaccgc ttaccttgtc ggcattttct ggctaattgc cctcatttgc aatttttctg
     1741 ccaaaaacca ccgcttgcaa tggctcggcg tgcttgccct catcgctttc ggctggctac
     1801 tccgctgggt actggtgatc caagtccaaa ccattgccaa aaccaatgcc ttgcaaaatc
     1861 catatcattt cgattggaca gccgtcgatg gtggtttggg gattgtctcg atttttgggc
     1921 taacggtgct agtaagtatc gtggtatggc aactgatttt aataatttgt gggggaagaa
     1981 aaggtgaaca ataaacgcag aaatctgttg aaaggcgggc tggcacttgg tggggcggcg
     2041 gcgtttgggg ccgggtattc gccgaaggtg tcggagattg ccaaaggggt gatgcacggc
     2101 acatcgggcg agaaaacccg tgatgcaatt cacggtaatt cgctcacgcc ggagtatcag
     2161 gtaaaagacg gcaaactgct cagcaatcct gagcaaattg tgtgcaatac acagtgtatg
     2221 ggctgttgga cgcagtgtgg cattcgggcg agggtggatt tgaacaccaa ccaagtgatt
     2281 cgcatttcgg gcaatccgta tcacccgctt tcctccgaca aaaccctgcc gttcggtatg
     2341 ccgattgcca aggcggaact gttgctggcg ggcgaaagtg gcattgaaaa tcgctccacc
     2401 gcctgtgcta gaggggcggc gttcttggag ggggtgaaca gcccgtatcg gattacccaa
     2461 ccgttgaaac gggtcggaaa acggggcgaa ggcaagtggc agaccatcag ctttgagcag
     2521 ttggtggagg aaatcatcaa tggtggtaac ctatttggtg aaggcgaagt ggagggcttg
     2581 aagacgatcc gcaatctggt cgatccgatt aacccgaaac agcctgattt gggaatgaaa
     2641 gcaaaccagc ttttagttac ttttgccggc cccgaaggtc gtcagccgat tttgcaacgc
     2701 tttgcccaaa agagcttcgg cacgatcaac ttttccaacc acggggctta ttgtggcgct
     2761 gcgtaccgtg ccggttcggg tgcctttatg aaagattttg acaaccaaca acacgccaag
     2821 ccggactggg atcacgctga atttattctg tttatcggca cgtctccggc acagtcgggc
     2881 aatccgttta agcgtcaggc tcgccagctt gccaaacgcc gccctgatga caatttcagc
     2941 tatgtggtgg tttcccctcg tttggagatg acctcctccc tcgccaccca aaacaaccat
     3001 tgggtgccga ttaagcccga gggcgatttg gcgttggtaa tgggaatgtt gcgttggatt
     3061 atcgaaaacg agcgtttcaa tgctgactac ctctcccgcc caagtctggc agcgatggaa
     3121 aatgccggcc acgtcagcta tgccaatgcg tcacatctga ttatttgcga accgaaccac
     3181 ccgaaattcg ggcaggcgtt gcggattagc gatctgcaag atgtgcaact cgaccccgaa
     3241 gagaaagtgg aagaaaacct gattgtggta aaagatcagg caagcggcga actcatttca
     3301 gcaaaagatt gcttacaagc ggtcattttt gtcgaacaaa ttgcaaaact gcttgatggc
     3361 tcggaagtgt tggtgaaatc ctcaatgcag ttgctgaaag aggcgacaca agagaaaacc
     3421 cttgccgaat atagcgacat ttgtggcgtg ccggtgcaaa ccattgaaaa cttagcgaaa
     3481 aaattcactt cgcacggtca taaagccaat gccatcactc acggcggcac gatgcacagc
     3541 accggttttt ataccagttg ggcgattttg ttgctgaatg caatggtcgg taacctcaac
     3601 aaaaaaggcg gaatggcagt agccgcaggc aaattcaaag atttcgggga cgggcctcgc
     3661 tacaatttag cgaacttccc gaatgaagtg aagccaaaag gcacgaacct tgcccgcagt
     3721 aaaaaggcgt accaaaattc aagcgaattt aagcagaaag tgacgaaggg cgaaaatccg
     3781 tatccggcac aaggggcgtg gtatccgttc accggcgggc aaactaccga gatgtttacc
     3841 tcaatggtgc agggctatcc atactttgcg aaagcgtgga tcaaccatat ggcgaacccc
     3901 gtttacggct taaccgctat gaacccgatt gcgttggaga aattaaaaga tccgaaaatt
     3961 ctaccgctct ttgtctctat tgatgctttt atgaacgaaa ccactgcact gacggactac
     4021 atcgtgccgg acacccacaa ctttgagagc tggggctttt caacggcgtg gggcggtgtg
     4081 cctcaacgca ccagcaccgc acgccacccg attgtgccgt ccaaaaacgc caaaactgcc
     4141 gaaggcgaca ccatctgtat ggagaatttc gtgattgcgg tggcgaaaaa aatgggcttg
     4201 ccgggctttg gcgaaaatgc gattgccgat ttagaaggca accgctatcc gctccaccgt
     4261 accgaagatt acttcctacg ggcggcagcg aacattgcct ttgccggtga gaaacctgca
     4321 ccggacgcca gcgagcaaga tctgctgctc accggcgtgc aacggattat gccgaaaatt
     4381 gaacaaacgc taaaagccga agagcgatta aaagtggcga atgtgtattg caaaggcgga
     4441 cgttttgccc cacacgaaag tgcgtggaaa gaggagaata tggagttcca gtggaaaaac
     4501 tgcctgcaaa tttggaacga aaacgtgtat aaagcccagc accacagcaa cggtgaacac
     4561 tattggggct gccctcgtta tatgtcgcca cgttttgcgg acggctccac gctggagcag
     4621 cactatccgc agtcggaatg gggctttaaa ctgatttcgt ttaaatcgaa cattatgagc
     4681 agtattaccg ccccactgtt gcgtctgctc tcgatcaagc ctgagggctt agtggcgatg
     4741 aaccgtttag atgcggaaga aaaaggcatt aaacacggcg atgtggtgaa actcagcacc
     4801 ccaaccgccg gcttgacggt gcagattgtg gtgttggacg gtatcgcccg aggcacaatc
     4861 ggcatcgaac acggctacgg tcataaacag attggggcaa gttcctacac cattgacggc
     4921 gtggaaattc cagccaaccc gatgatcgcc aaagggatta acctcaacga cctcggcatt
     4981 atcgaccaca ccaaagcggt caaatccccg tgggtagatt gggtctgtgg ctcagcggtg
     5041 agaaatggca ttccggcgaa ggtagagaaa ttagcctagc cgaacaagcg gttggttttg
     5101 cagaaaaatg tgcaaaatcg accgctttta ttgatgtaag ggacactgtt ccttatgcaa
     5161 accagcattg cagagcgtaa aaaatcatac tctgcatttt aatttatttt ggagaaccta
     5221 tgacttttgt caaaaaaaca gcactgagcc tgattgcaac ggcacttttc gctacatcaa
     5281 gttttgccgc tgagcagagc caataccaac atattcgtaa tgcaacggcg aaaattgaat
     5341 atgccggcaa gacgtttttg gttgatccgt atttagcgga gaaagatcgc tacttaccgc
     5401 cgaatgcgaa aaaacgtaat ccgaccttgg atatgactgt gagcattgat gaagtgttaa
     5461 atggcgtgga tgcggtgatc gtaactcaca ctcaccacgc tcactgggac gatgtagcac
     5521 aacaacgtgt gccgaaaaat ctgccgattt ttgtgcagaa tgccggtgat gcccgtttaa
     5581 tccgttcaca aggctttacc gatgtgcgtg tggtcggcaa agataccccg tttgaaggcg
     5641 taaccttaac ccgaactcgt ggccagcacg gcaccgatgc gatgtatgcg aacccgaaaa
     5701 ttgccgaaat ggtcggcgat gcgatggacg tagtgatgaa agcggaaggc aaaccgaccg
     5761 tctatttggt gggagatacc atttggaacc acgaaatcga acattcgttc aacacttaca
     5821 aaccagactt tgtggtgctg aatgccggct gtgccaaact accaaacttt gaaggcagcc
     5881 cgattatgtg taccgaagat gtttccaaag cacacaaaat cgctccacaa gcgaaaatct
     5941 taactgtgca tatggacgcc ctgccacaaa cctcaatcag cagtgatcag atgcgtgatt
     6001 atgtacaaaa agagggatta aatcgtgtaa ctgtgccgaa aaacggtgat gtgattaagt
     6061 tctagtagct aaactaacat taccaaaatg gtttaagtag caataaatag cgaagcgggt
     6121 taagccatca agccttaacc cacttttacc aaccatagta tatagtttta gatataatta
     6181 tcttctgtat ttacctgcca ttgcttggta aaccgcattc tcgttggtca aaatcgcata
     6241 ttttgcattt aaaatcgcca actgagaatt aagctcggta ttcagtgcgg taatccactc
     6301 acggaactcc gacacccctt gttcataacg gtttttatag tatgaactga tacgtttatt
     6361 tttcgcattg gtatcttgaa ggatttttaa cgagcttctc gactgcgtat aactgtaata
     6421 ataacggtca atttcattta aggctgaggt aattttttgc tcatagttta aacgggcagt
     6481 cacataatcc gtttcagaaa tttttacgtt atttttaacc cgattccaat ctaagaacgg
     6541 taaatccaat ttaattaagc cgttacccac agggttatct gccacattgc cgatattggt
     6601 tgcactaccg gtaatagaac caccgatggt taaggttggg aaccagcttt tctcggttgc
     6661 ggttaaggtt ttaaatgcac tttgtaagcg ttgtaacgag cctactacat cagggcgatt
     6721 agcaatggct gacactggca catttaaatc taccccttgc aatttcacgc tcataatatt
     6781 cgggtaacga atgttaagcg gagtatttgg ctgaacattt aacaaattgc gtaatgtttg
     6841 ctcggcaatt ttttggttgg ttttcagatc aattacacta tttctggcgt ttaagaccgc
     6901 ttgttgagcc tgatcaaccg ctaaactatc aatcgcaccg gcattcattt tgttagttaa
     6961 aatacggctg atttgctcat aatggcgaat gctctcttcg gtaatacgaa tcgcatcacg
     7021 atagtaagct aactggtagt aagtagtcac aaccgcatta ataatagata aacgggtagc
     7081 ttttaagtct tcaatggtgg ctttatgcgt ccattctgcc gcactggcgg tatcggctaa
     7141 acgtcgccat aaatccacag tataacttaa attaaatgca gcttgagctg cggttgttga
     7201 aacaccggtc gaaacattat ttgaagaagc cgaacctaaa cctttagccg cagaaacaga
     7261 acctgaacca ctaaaggttg gcactaaatt tgcccctact aaattagcgt tatacaaggc
     7321 tttattgact gcaattccgc ttttggctaa atcaaggtta tttttgatgg ctaaatcaac
     7381 cacacggttt aattccgcat ccgtatagcc ttgccaccat tgctcgttaa tttgatattg
     7441 tttggtaatt tcatcatagc tttgataggt ttgctgtgct tgttgcaaag tcccgtcatt
     7501 gctcatattc cctgtggtac aagccgttac ggttagagcc aacgccacag aaaatgctaa
     7561 ttttgaaagt ttcattacat ttccttttga aaataaagag gggaaaatgg gcgtattcta
     7621 cccaaaaaca gattgccttt ccagttaaat gaacaactgt tcatttaatt atttaacaaa
     7681 tcagtaatca aatgctgatg acgaatttgt tgcgaaccta agctcaaacg ctcggcaatt
     7741 aaaatcgctt ttagctctgc caatgcctgt tggaaatcat cattaataat cacataatcg
     7801 aactcattgt aatggttcat ttccgacacc gcctctgcca tacgtttggc aatggtttca
     7861 gcggaatctt gcccacgccc gattaaacgt ttttccaatt ctgtttttga cggtggcaaa
     7921 ataaaaacgg ttttcacatt cggcactttt tcccgaattt gtctagcccc ttgccagtca
     7981 atatccaaaa agacatcaat gccctgctct aggctacgtt caatcatcgg caatgatgtg
     8041 ccgtaataat tgccaaacac ctccgcccat tctaaaaaat acccttgctc aatcagctgc
     8101 ttaaactcat cgtgcgaagt gaaataataa tgcacgccgt cctgttctgc cggacgaggc
     8161 tgacgggtag tgtgtgaaat cgaaagctgt acttccgaac ggggtaaatc cgccaacaag
     8221 gcgttaatta aggaagattt tcctgctcca cttggggcag aaataatata aagattgcct
     8281 aaactcatta tttgtatata cctatttgat tttgggaata gggcgaatga tgattcgccc
     8341 ctacatttca taggaataaa taaaaaataa tggacaactt tcattgtcca ttacacaaaa
     8401 ttatctacgt gccaaaggct gaacgaaggt aatgcctaaa tcccaaggct gctcaatcca
     8461 cgtttcttgt ggaatatcaa tcacataatc atcaaccaac ggtgcaccgt gcggtttcgc
     8521 aaacacggtt acaaaacgag ctttcgggta cattctgcga atttcttttg ccgtgttgcc
     8581 ggtatccact aaatcatcta ccacaataaa gccttcgcca tcggtatcgg ctgcgtgtaa
     8641 cacatttaac tcaccttggc tatcgtgatc gtagctggca atgcacactg tttctacgtg
     8701 gcgaataccc aactcacgtg ctaaaacggc tgccggaaac aagccgccac ggctcaccgc
     8761 aataatccct ttccactgtg tggcaggtaa taaattttct gccaacttgc gagcgtgcat
     8821 atggaacata tcccacgtca cgacatattt ttcatttgtg tatttttcac tcatagaaac
     8881 aacctaatat aaaattgggg gctaccccga tataaaaaat tgcactagga taacccgaaa
     8941 atgctttttt cgccatcatt tcatttattt ttttgtgccg gcagctacgc aatcgtatgc
     9001 ttagcacaaa aaaccaacac taaaatagct tttctaaagt gaaaaagact tccttgtttt
     9061 ggcgagagta aaaatcagca atattgctgt caattttttg ataacggaaa ttcagcttcg
     9121 gcacaatgcc ctgccaatgc caagtgcggt tccaaaccgc taaattggct tgatattccg
     9181 tgtctaaccg cttatagcca aacaaatagt gcttgtcggc aaaatatcgc ttgccaaagc
     9241 cgatacttgc ctgcgttgcc cagcgttcgc cttgaaaaac cgcccccact cgagccagcc
     9301 acttattcga gctttgtgct ttttcccgtg caatttcacg cacgccgtct aaatttgcaa
     9361 aaaaccgccc atttttgacc gcttgccact gcacaccgcc actgagttgg tgctgatagc
     9421 tgttataacg gcttgcggtt agctcctcaa aataccgttt ttgcaaatgg gtaaaactcc
     9481 ccgacaccaa ccattgcgaa ttaaggtaac gcaaaccgtt gagtgttgcc ccaaattgaa
     9541 cattataacg gctactcccc agccaatttt gctcaataaa aggcgaaaaa gtcagccgat
     9601 aagcgacggt gcggtagcta taattcggcg aaacggatac ggattgctca cggtaatctc
     9661 ggttatccca atagtaaatc ccgctataat tgagcgttaa accgacaaaa tgattgcctt
     9721 tcacgttgtg caatttactc aaccctaagc cataacgcac gccttttgct gattgtggca
     9781 aactgtctgc atttttgcgg aaaatccgcc catttacatt cacaatcggc gaagatgagg
     9841 cattattcac gttatctgtt tgggtatatt gcagataaaa atcgggctgc cacgcctcat
     9901 tttcagcaat ttggacttga gtattgcggg caattttagc aagttccggc ggtaaatggg
     9961 cttgaatagc cttaaattcc tgctctgccg cacgaaattg ctgatcttct gccaaaatca
    10021 acgccaaatc aaaacgaggg taaaccaaac tcgggggctt agctaacagt tggcgatagt
    10081 gaaaaactgc ggtttgatga tcttttttcg cataggcaag tgcggcttgc gcatagtgcc
    10141 acaacaacgg gtcaaaattt tcagtttgcg gataacggct taaaagcggt tcaagcgttg
    10201 cccaatcttg gctgagaatc gcttggttaa tttgctgttc aacattctga ttgactgaat
    10261 acgcaggctg ataagcggtc ggtttttgtg aattttttac aacattttgc tcaaatttaa
    10321 ccgcttgcgg ctgacgctca aaggaaatcg ggtcagtgtt gtgttcaatc accttttctg
    10381 cccatacaac ggggctgaac agcgaaaata aaaggggata ttttttcata aagataaaga
    10441 aaattggtag gggcggacct atatgttcgc ctctacggga tttatcactc tctccccttg
    10501 tgggagaggg ttattagaag ctgtaaattg aaatctaaac tattgtgtct tattgtatct
    10561 acaagcaata tcaagacaaa tgccgtttaa tttttcgtta aacggcattg aacgttagaa
    10621 aaggttattt ttggatatcg ccacgagtgc cggcaagtcc aaaattactc acttccccag
    10681 taagatttgc ttttgtctga atttcaccgg caatttcctc tgcattttta ccgaaaaatt
    10741 cgactgtata ttttccagaa aggtttaaag gattattgcc atgtactgtt gaagaaatct
    10801 gagtattatt aatcgtcccc tcttctaata cgattaagtt tccaaattct ccctcaactc
    10861 tacctgaacc ggtacgctta gagaaattaa cattataggt tagatcacca ccattaaaac
    10921 ctttagctcc atcgtggtta aatgcttttc ctgaatatgt tgcagtaccc tctgttggta
    10981 atttctctgt tttcaatcct ttaactgtaa ctgttccatc aactttatca atataattac
    11041 cgttttccca gccatttctt tgaactgctt gtgctgttac tacagaatat ggctgattat
    11101 ataaataact ctgtgttctg actgtgactt taccctcaat agtttcttta ctattaccaa
    11161 gttttaagcc gtaatttgta tcatcaatac cttttgcttt aataatagct ataggatcta
    11221 cttctttttc tgctgctaaa cgctccgctt ccgctttctc tttcgccgct ttttctgccg
    11281 ctaaacgttc tgcttccgct ttctctttcg ccgctttttc cgccgctaaa cgttctgctt
    11341 cggctttttc tttcgctgct acgttccgct tcggcttttt ctttcgctgc tttttccgcc
    11401 gctaaacgtt ctgcttccgc tttctcttta gctgcttttt ctgctgctaa acgttctgct
    11461 tctgctttct ctttcgctgc cttgtctttt gctgcttttt ctgctgccgc tttatcggtt
    11521 tgttgctgag tagtttgcgg tttagtattc ggttttgcca cattgtttga gctactgccc
    11581 gaaccaccac tactacacgc cgttaaaccc aataaaatca aactggctaa agttactttt
    11641 ttgatagaca taaaatgttc cttttg
//
LOCUS       DANLYI010000061        11660 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.061, whole genome shotgun sequence.
ACCESSION   DANLYI010000061 DANLYI010000000
VERSION     DANLYI010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 11660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11660
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.061"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            36..254
                     /locus_tag="PXD81_001205"
     CDS             36..254
                     /locus_tag="PXD81_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015702330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL5537232.1"
                     /translation="MWLRELFLKRRLELGLTQRTLGEKMGVLYSFIGKVETGDRRLDI
                     FEFIAYCKGLDLDPLSVLQEIEQQFPHK"
     gene            complement(301..921)
                     /locus_tag="PXD81_001206"
     CDS             complement(301..921)
                     /locus_tag="PXD81_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HDL5537233.1"
                     /translation="MKLWYSTTSPFARKALAVIKHHQLDDKVEMLRVTKAMDPNSPHN
                     QDNPLGRIPALQRNCGRWLFGSLLISEYLDQKGNNTPLLPKEGKPRWAVLALHNLADG
                     IMENTMPTLLQERMARPENEWWVSRHEQLMERNIRSFRQLEEALQEFGTELNLGTLTA
                     VALIDWWAFRLDKIGYDLAKNYPNLTAWAEEMNNKYAILAETKPSM"
     gene            complement(923..1576)
                     /locus_tag="PXD81_001207"
     CDS             complement(923..1576)
                     /locus_tag="PXD81_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01621 family pseudouridine synthase"
                     /protein_id="HDL5537234.1"
                     /translation="MTPTLPICYQHRDFIIINKPEGLSVHKDEAEIGLTELVARQLNV
                     KQVWLVHRLDKITSGLLILALNKEAAARFYHLFEQHKIYKTYWALSDKKPKKKQGRIV
                     GDMQKSRNGAWKLCHSKENPAVTQFISHSVEDSLRHFILQPKTGKTHQLRVAMKSLGS
                     PILGDKLYSGSQADRVYLHAYQLDFEYENEKISVQALPTSGHFWQKICKSNHIKHGE"
     gene            1625..2284
                     /locus_tag="PXD81_001208"
     CDS             1625..2284
                     /locus_tag="PXD81_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HDL5537235.1"
                     /translation="MAVTIYLVRHGRTVWNLEGRLQGSGDSALVEEGIIGAKKTGIAL
                     KHIPFTAAYSSMQKRAQDTANYILAENERSNIPHFHHKGLNEFDFGSWEGMKSVDLQE
                     NDEYWVMKRTPAEYLAKANGGERFEQLYQRVTQAFNQIAELHQNDGKVLIVSHGMTLT
                     LLTAVLKGIAWQDFRNEEKHSFVFNTAITKVEVENGKVTVLEFNNTDHLDNVPTSQYK
                     R"
     gene            complement(2321..3367)
                     /locus_tag="PXD81_001209"
     CDS             complement(2321..3367)
                     /locus_tag="PXD81_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01620 family protein"
                     /protein_id="HDL5537236.1"
                     /translation="MSKQIFNEEELKTTEHFEPKQEFDLENAIIEEEEKAVEAELIIE
                     ESLKPSRFWIRVLLATLALFGVAVIAQSIQWLIDTFQQREWIYFAFSIVFFIVSLLGV
                     GAIISEWRKLVYLRNHQKNQQMSNQLLLEQLPSNDGEQAVKFCENIANQLNHLPSIAQ
                     AEQLWKSELNEAYNAKEVLYLFSENVLKPIDKQVKQMISRNAAENAVIVAVSPLAIVD
                     VLLMAARNIALVNKITKAYGMELGYISRLKLFKMVLKNMVFAGATEIATDVGMDFFSQ
                     NLTAKLSLRAAQGIGVGLLTARLGIKAMEFCRPVAFQANEKPRISAIRQELLTSVKNT
                     VFAKTETKEKVFSD"
     gene            complement(3367..4320)
                     /gene="dusC"
                     /locus_tag="PXD81_001210"
     CDS             complement(3367..4320)
                     /gene="dusC"
                     /locus_tag="PXD81_001210"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HDL5537237.1"
                     /translation="MNPIPNVILAPMQGVLDPFVRNLLTSVNDYDLCISEFVRVVDQR
                     LPKKTFYRLAPELYQGGLTDSGTPVRVQLLGQHPQWLAENAALAIELGSHGVDLNCGC
                     PSKTVNGSNGGASLLKDPELIYRATKAMREAVPSDKIVSVKVRLGWDSVSQCFEIADA
                     VEQGGANEITVHGRTKVDGYRAERINWQAIGEIQQRLSIPVIANGEIWDFESAKNCQK
                     ITACKALMMGRGALNTPNLSKVVKYNVPKMAWNEVLQLLFKYVQMENQFDTGFYHVAR
                     IKQWLRYLDKEYPQAVELFDILKTEHGYDGLKAHIEKAVEE"
     gene            complement(4398..5024)
                     /locus_tag="PXD81_001211"
     CDS             complement(4398..5024)
                     /locus_tag="PXD81_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HDL5537238.1"
                     /translation="MDSRSHIIPLFLAIVITVIAVWSGLNPTDRAVWYAEVIPIFIVF
                     ALLIITYPKFQFSGLAYFLMSLWLIMHLIGAKYTFANVPFEWANQFLTPILGEDRNHF
                     DRVAHYIIGFYSFPMAEWLLRKRKCGMGVAFFFSLFFIMSVAAAYEIIEWQYAVIEGG
                     NAGVEFLGSQGDIWDAQKDMLADTLGALTALMIYLIARPDLRIHDRHF"
     gene            complement(5024..5881)
                     /gene="djlA"
                     /locus_tag="PXD81_001212"
     CDS             complement(5024..5881)
                     /gene="djlA"
                     /locus_tag="PXD81_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005609757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HDL5537239.1"
                     /translation="MQFIGKIVGFFLGYQLFHGLFGGILGAFIGHLADKKLYELGSVR
                     SSLFGKNLTRQSLFTQTTFAVLGHIAKAKGRVTEADIELARALMARLKLDSAAQQLAQ
                     NAFTLGKEPDFPLRQVIQEFREACGARTDLLRFFVEVQMQAALQDGQLDVNEQQILFT
                     IAETMGMSRFQFEQMIAMIMAAQQFRNGGYYQQNGSYHQQSQGGYQGSYQQPAQPSIE
                     AAYTVLGVTAQDDQNTVKRAYRKLMNEHHPDKLAAKGLPDEMMELAKEKAQQIQAAYD
                     LICKVKEWK"
     gene            complement(5929..6639)
                     /locus_tag="PXD81_001213"
     CDS             complement(5929..6639)
                     /locus_tag="PXD81_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDL5537240.1"
                     /translation="MWKNRQLFFGYAENRLLLLSRSITGLLGILCGVYIIDHLVLSDV
                     DMIGKLTSFILIILSAIFLKERASLLQWILCLIAFIGALFIIKPAVDIRFMPYLVGVI
                     GSVFAAIAYLCLRLLAKTKKVESPNTIVFFFSAFSTLILLPFVAIDYVVLTNLQTIYL
                     VLAGVTSAVGQFCVTTAYKYAAAKDISIYSYASVLFSAILGLLFFEQYPDMLSWLGYA
                     IIFVSSWLMFVLTKKQSK"
     gene            complement(6654..6806)
                     /locus_tag="PXD81_001214"
     CDS             complement(6654..6806)
                     /locus_tag="PXD81_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5537241.1"
                     /translation="MNFWNSLSPLFKAIFCVLLSALSFAIMAAFLRLSGDVPIAQKAI
                     FRNAQH"
     gene            complement(6875..7360)
                     /locus_tag="PXD81_001215"
     CDS             complement(6875..7360)
                     /locus_tag="PXD81_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HDL5537242.1"
                     /translation="MKKFAKVLMVAVPAFALAACSSSSDDAAANAAANAAANAAANAA
                     GEFGGLTAEQLKAQYNTVYFGFDSYAVEGEYRQLLDAHAQLLAKTQGNVTIAGHADER
                     GTPEYNIALGQRRADAVKGYLAAKGANNTSTVSYGEEKPAVLGHSEADYAKNRRAVLE
                     Y"
     gene            complement(7387..8673)
                     /gene="tolB"
                     /locus_tag="PXD81_001216"
     CDS             complement(7387..8673)
                     /gene="tolB"
                     /locus_tag="PXD81_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolB"
                     /protein_id="HDL5537243.1"
                     /translation="MKLFSRISGVIGIFSALLTTQVVADSDVRISIDEGVSMAQPIAV
                     VPFKASGVPADVAQIISDDLRNSGKFTPLERSQMPAQPGSAAEVVADQWSAIGIDNIV
                     VGQVSSTGGGYNVAYQLIDTLGTSGTAGGVIAQGSFIVPAAQIRQGAHTVSDEVFEKL
                     TQIRGAFRTKIAYVVQRGVSSYELRVSDYDGFNAFTITTSKEPLMSPEWSPDGSRLAY
                     VTFENKKAQVVVHDIRSGARRVVAALKGHNGAPSFSPDGSRIAFASNQDGELNIYVVG
                     SGGGTPSKLTANAGNNTEPSWSPDGSTIYFTSDRAGSPQVYRMSSSGGGASPMGGSGS
                     YNAKVSSDGKNLIMIAGDRVVKRDLASGGTEVLSSTFLDESPSISPNGIMVIYSSTKG
                     TSKVLQLVSADGRFKANLPGAGGHYKFPAWSPYLTK"
     gene            complement(8689..9912)
                     /gene="tolA"
                     /locus_tag="PXD81_001217"
     CDS             complement(8689..9912)
                     /gene="tolA"
                     /locus_tag="PXD81_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HDL5537244.1"
                     /translation="MKSQQDRLELAVIVSILLHILLIGLLLLGSLFTKTELAAAGGSG
                     GDSDSFEAVMVDTGQVAAEYGQLLAEKKGSKASKPKVEPKEEAVEEDVEDKPTPEEIQ
                     KQKQAELAAIQEKQRQQEIERQQKLQEQQKQEQAKQEELKKQQAEEATRKKAAEAAKL
                     KADAEAKRLEAAAKQAEEEKKAKEALEKKLEQQKKAEAEKKLKEQKELKEQQAKEAKE
                     AKEKAEKEAKAKAEKEAKEKAEKEAKAKAEKEAKEKAEKEAKAKAAAEAKAAQAKNNK
                     ALDDFLNSGDIGGGSSKGGNKNSAGSQGSGGTSGLGQGTNVDGNAYGQRIKKLIQSRY
                     RVDPSFAGKQCDVKIFLSRDGTITNYQVISGDKAVCDAAVSAIVATRKVPPAPSDAVY
                     NMFKSPTLDFSLKVK"
     gene            complement(9926..10363)
                     /gene="tolR"
                     /locus_tag="PXD81_001218"
     CDS             complement(9926..10363)
                     /gene="tolR"
                     /locus_tag="PXD81_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HDL5537245.1"
                     /translation="MSRRLKRNDIKSEINIVPFLDVLLVLLLIFMATAPIISQSVEVN
                     LPEDTHSQSVSNEDKTPVILEVAGVGEYKLKIDGNYVQSNGQENINESEVVAYAGEQF
                     QKDNNTLFLVAGDKVVPYEEIMKGITLLKDAGVKSVGLMTEGK"
     gene            complement(10505..11185)
                     /gene="tolQ"
                     /locus_tag="PXD81_001219"
     CDS             complement(10505..11185)
                     /gene="tolQ"
                     /locus_tag="PXD81_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolQ"
                     /protein_id="HDL5537246.1"
                     /translation="MQTEFSLLSLFLEASIVVKIVMLILIVFSILSWAVIIQRSKILT
                     KAKKDSLAFEDRFWSGEDLNRLHQGLENRRDALNGSEQIFYVGFKEFTRLQQANPDAP
                     ESIIQGSTRAMNLALNREMESLENYIPFLGTVGSISPYIGLFGTVWGIMHSFMGLSAV
                     KQATLQSVAPGIAEALIATAIGLFAAIPAVMAYNRLNLRLGKVEQGYVNFIDEFTTLL
                     HRQAFSKR"
     gene            complement(11207..11608)
                     /gene="ybgC"
                     /locus_tag="PXD81_001220"
     CDS             complement(11207..11608)
                     /gene="ybgC"
                     /locus_tag="PXD81_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HDL5537247.1"
                     /translation="MDFPIRVYYEDTDAGGVVYHSNYLNFFERARTEFLRQQHFSQQE
                     LFSQSLAFVVKRIEIDYKLPARLDDLLNVETTLSELKKATLIFKQRLWKDGDCLSEAN
                     VTVACVDLIKMKPVAIPDDIRQALQKSYFSI"
BASE COUNT         3363 a         2632 c         2131 g         3534 t
ORIGIN      
        1 aaaatcttag attatcaata cattccagcg agcatatttg gttaagagaa ctattcttaa
       61 aacgccgttt ggaactaggg ctaacacaac gcacattagg agaaaaaatg ggcgttcttt
      121 actcatttat cggaaaagta gaaacaggag atcgcagatt agatattttt gaatttatcg
      181 cttattgcaa agggttagat ttagatcctt tatctgtatt gcaagaaatt gaacaacaat
      241 ttcctcataa ataaaggcat tgagttataa ctcaatgcct ttattttcta tgtcaaatct
      301 ctacatgctc ggcttagtct ctgccagaat ggcgtattta ttattcattt cctctgccca
      361 agcggttaaa tttgggtaat tttttgcaag atcgtagcct atcttatcta agcggaatgc
      421 ccaccagtca attaatgcta ctgcggttaa tgtgcctaaa ttcagctctg tgccaaactc
      481 ttgcaatgcc tcttctaatt gtctgaacga acggatattg cgttccatta attgctcgtg
      541 gcggctgacc caccattcat tctcggggcg agccatacgt tcttgtaata aagttggcat
      601 cgtattttcc ataatgccgt ccgccaaatt atgtaatgcc aacactgccc agcgaggttt
      661 accctcttta ggtaacagtg gagtgttatt gcctttttgg tcaagatatt cgctaattaa
      721 caaactaccg aaaagccaac gtccgcagtt gcgttgtagg gcaggaatac gtcctaacgg
      781 gttatcttga ttatgtggcg aatttggatc cattgctttt gtcacccgca acatttcgac
      841 tttatcgtct aattggtggt gtttaatcac cgcaagggct ttgcgggcga aagggctggt
      901 ggttgagtac caaagtttca tactattctc cgtgtttgat gtgattggat ttgcaaattt
      961 tttgccaaaa atgaccgctt gtcggcaagg cttgcactga gattttctca ttttcgtatt
     1021 caaaatcaag ctgataagcg tgcaaataaa cacggtcggc ttggctacct gaatagagtt
     1081 tatcacctaa aatcgggctg cccaaacttt tcattgccac tcttaattgg tgagttttgc
     1141 ctgttttggg ttgcaaaata aaatgcctga gcgagtcttc aacagaatga gaaataaatt
     1201 gcgttactgc cgggttttcc ttactgtggc ataatttcca cgctccattt ctgctttttt
     1261 gcatatcgcc aacaattcta ccctgctttt tcttcggctt tttatcgctt aatgcccaat
     1321 aggttttgta gattttatgc tgttcaaata aatggtaaaa ccttgctgcc gcctctttat
     1381 tcaacgctaa aatcagtaaa cctgaggtga ttttatccag acggtgaacc agccaaacct
     1441 gctttacatt cagttggcga gccactaatt ccgttaagcc gatttcagcc tcatctttat
     1501 gcacactcaa gccttccggc ttattgataa taataaaatc acgatgttga taacaaatag
     1561 gcaatgttgg ggtcattttg ctataatcct tccaattttt gactatttta gcaaggaaat
     1621 tacaatggct gttaccattt atttagtacg acacggcaga accgtgtgga atttggaagg
     1681 cagattacaa ggctcgggcg attccgcttt agtagaagaa gggattatcg gagcgaagaa
     1741 aaccggcatt gcgttaaaac atattccgtt taccgccgcc tattccagta tgcagaaacg
     1801 ggctcaagat accgcaaact acattttagc agaaaatgaa cgcagtaata ttccgcattt
     1861 tcatcataaa ggcttgaatg aatttgattt tggcagctgg gaaggaatga aatctgtcga
     1921 tttgcaagaa aatgatgaat attgggtgat gaaacgtaca ccggcagaat atctggcaaa
     1981 agcaaacggt ggcgagcgat ttgagcaact ttatcaacgg gtgactcagg cgttcaatca
     2041 aatcgccgag ctacaccaaa atgacggcaa agtgctgatt gtttcacatg gtatgacctt
     2101 aaccttactc acggctgtgt taaaaggcat tgcgtggcaa gatttccgca atgaagaaaa
     2161 acatagcttt gtgttcaata ctgccattac gaaagtagaa gtagagaatg gcaaagtaac
     2221 ggtgctggaa tttaataata ccgaccattt ggataatgtg ccaacaagcc aatacaaacg
     2281 ataatattaa gggcggaaca ttgtccgccc atagcatttt ctaatcagag aacacttttt
     2341 ctttcgtttc tgttttagca aataccgtat ttttgaccga agtaagcaac tcctgacgaa
     2401 tagctgaaat tcttggcttc tcattcgctt gaaaagcaac aggacgacaa aattccattg
     2461 ctttaatgcc aaggcgagca gtgagtaaac ccacaccaat accttgtgcg gcacggagcg
     2521 ataatttcgc cgtaaggttt tgcgagaaga aatccattcc aacatcagta gcgatttcgg
     2581 ttgcaccggc gaacaccata tttttcagca ccattttgaa cagttttaag cggctgatat
     2641 aacccaattc cattccatag gctttggtaa ttttattcac taaagcgata tttcttgccg
     2701 ccattaacaa cacatcaact attgccagcg ggctaaccgc cacaatcacc gcattttctg
     2761 ccgcattgcg ggaaatcatc tgcttaacct gtttatctat cggctttagc acattttcgc
     2821 taaacagata tagtacctct ttggcattat aagcctcgtt cagttcgctt ttccaaagtt
     2881 gctcggcttg agcaatgctc ggcaagtgat taagctgatt tgcaatattt tcgcaaaatt
     2941 tgaccgcttg ttcgccatca ttggaaggca gctgttccag caataattgg ttactcattt
     3001 gttgattttt ctgatgattt cgcaaataca ccaactttcg ccattcggaa ataatcgccc
     3061 caacgcccaa caagctgaca ataaaaaaga ctatcgagaa cgcaaaatag atccactctc
     3121 tttgctggaa cgtatcaatc aaccactgaa tactttgagc aattaccgcc acgccaaaca
     3181 aggcaagtgt ggcaagtagt actcgaatcc aaaagcggga aggcttaagg ctttcttcaa
     3241 taatcagctc cgcctcaact gctttttcct cttcttcaat aatcgcattt tcgagatcaa
     3301 actcttgttt aggttcaaaa tgctcggttg tttttagctc ttcttcatta aaaatttgct
     3361 tactcattat tcctccactg ccttttcaat atgtgctttt agaccatcat agccgtgttc
     3421 ggttttcaag atatcaaata attctaccgc ttgaggatat tctttatcca aataacgtag
     3481 ccattgtttg atgcgagcaa cgtggtaaaa accggtatca aactgatttt ccatctgaac
     3541 gtacttaaac aatagctgta gcacttcatt ccacgccatt ttcggaacat tatatttcac
     3601 cactttgctt aaattcggtg tgtttaacgc cccacgcccc atcattaatg ccttgcaagc
     3661 ggttattttt tggcaatttt tcgccgattc aaaatcccaa atttcgccgt tggcgataac
     3721 aggaatagac agccgttgtt gaatttcgcc aatcgcctgc caattaattc tttctgctcg
     3781 ataaccatca actttggttc gcccgtgaac ggtaatttca ttcgctccgc cctgttctac
     3841 cgcatcggca atttcaaaac attggctaac ggaatcccaa cctaatcgca cttttacgga
     3901 aacgatttta tcgcttggca cagcttcacg catcgctttg gttgcacgat aaatcagttc
     3961 cggatctttt aataacgaag ccccaccatt gctgccattt accgttttag aggggcagcc
     4021 acagttgaga tctaccccgt gtgaaccaag ctcaatggca agagctgcat tttctgccag
     4081 ccactgtggg tgttgcccta aaagctgtac ccgcactggt gtgccggaat cggttaatcc
     4141 gccttgatat agctcagggg ctaggcgata aaaggttttc ttcggtaaac gttgatcgac
     4201 cactcgcaca aattcggaaa tacagagatc gtagtcattc actgaagtta aaaggttgcg
     4261 gacaaaaggg tctaaaaccc cttgcatcgg ggcaagaata acgttaggaa ttggattcat
     4321 tggtaaaata aaaaatacaa gcggttattt tttctcaaca ttttgcaaaa aattggcaag
     4381 aaacaaccgc ttgaaggtta aaaatgtcta tcgtgaatgc gcaaatcggg gcgagcgatc
     4441 aggtaaatca tcagtgcggt taatgcccca agagtatccg caagcatatc tttttgagcg
     4501 tcccaaatat ccccttgcga gcctagaaat tccacgccgg cattgccacc ttcaatcacg
     4561 gcatattgcc attcaataat ttcgtaagct gccgccaccg acataatgaa aaacagcgag
     4621 aagaaaaatg ctacgcccat tccgcacttg cgtttacgca gtaaccattc tgccatcggg
     4681 aagctgtaaa aaccgataat gtaatgtgct actcgatcaa aatggttacg atcttcacct
     4741 aaaatcgggg ttagaaattg atttgcccac tcaaacggca cattggcaaa ggtatatttt
     4801 gccccgatca agtgcataat cagccaaagc gacatcaaaa aataagctaa cccactaaac
     4861 tgaaatttag ggtaagtgat aattaaaagg gcaaaaacga taaaaatcgg tatcacttcc
     4921 gcataccaaa ctgcgcggtc tgtcggattt aaccctgacc aaaccgcaat cacggtaata
     4981 acaatcgcta aaaatagagg gatgatatgc gagcgtgaat ccattatttc cactctttca
     5041 ctttacaaat taaatcgtac gccgcttgaa tttgttgagc tttctctttt gccaactcca
     5101 tcatctcatc aggcaaacct ttagcggcta atttatccgg gtgatgttcg ttcattaact
     5161 tacgataagc ccgtttcact gtattttgat cgtcttgtgc ggttacgcct aatacggtat
     5221 aggctgcctc aatgcttggt tgtgcaggct gctgataact gccctgatag ccaccttgag
     5281 attgctgatg ataactccca ttttgctgat aatagccacc attgcggaat tgttgagctg
     5341 ccataatcat tgcgatcatc tgctcaaatt ggaagcgaga cattcccatt gtttcggcaa
     5401 tcgtaaacag aatttgttgc tcgttcacat caagttgccc atcttgcaag gctgcctgca
     5461 tttgcacttc gacaaagaaa cgtaataagt cagttcttgc cccacaggct tctctgaact
     5521 cttgaatgac ttggcgtaac gggaagtcag gctctttacc taaggtaaag gcattttgcg
     5581 ctaattgctg ggctgcacta tctaatttta agcgagccat taacgctcgg gctaattcaa
     5641 tatccgcttc agtaacacgc ccttttgctt tcgcaatatg ccccaacacc gcaaaagtgg
     5701 tttgggtgaa aagcgattgg cgagttaaat ttttaccgaa aagacttgaa cgaactgaac
     5761 ctaattcata gagtttttta tcggctaaat gcccgataaa agcgccaagt atcccaccaa
     5821 ataaaccgtg aaaaagctga tagcccaaga aaaaaccgac tatttttccg ataaattgca
     5881 tttttttatc cttttataga aatgaataaa cgttattcta acaaaatttt actttgattg
     5941 ctttttagtt aatacaaaca taagccagct tgagacaaaa ataattgcat aacctagcca
     6001 actcaacata tccgggtatt gttcaaagaa caacaaccct aaaattgcac taaatagcac
     6061 agaggcataa ctataaattg agatgtcttt cgccgccgca tatttatagg cggtagttac
     6121 acaaaactgc ccgaccgctg aagttacccc tgctaacact aagtagattg tctgtaaatt
     6181 ggttagcact acataatcaa tggcgacaaa aggcaataat atcaaggtcg aaaaggcaga
     6241 aaaaaagaaa acaatggtat taggtgattc tacttttttg gtttttgcca ataaccttaa
     6301 gcataaatag gcaatggctg caaacacaga gccaatcacg ccaaccagat aaggcataaa
     6361 gcgaatatca actgccggtt tgataataaa aagtgccccg ataaaggcaa taaggcataa
     6421 aatccattgc aggagagatg ctctctcttt taaaaaaatc gcagaaagaa taattaaaat
     6481 gaacgacgtg agtttgccaa tcatatcgac atcacttagc acaaggtgat cgatgatata
     6541 tacaccacat aaaatcccca ataacccggt gatggaacgg cttaataata acaaacgatt
     6601 ttctgcataa ccaaagaata attggcgatt tttccacaca atataaccgc ttatcaatgc
     6661 tgtgcgttgc gaaaaatcgc tttttgtgca atcggtacat cccccgaaag acgaagaaaa
     6721 gcagccataa tcgcaaagct tagtgccgac aaaagcacac aaaaaatggc tttaaataac
     6781 ggcgagagcg aattccaaaa attcatagca taaaggcttt taataaaaat aaagccctga
     6841 ttattcaggg cttcaattta gaatcaacta agaattagta ttctaatact gcacgacggt
     6901 ttttcgcgta gtcagcttct gagtgaccta atactgctgg tttctcttca ccgtaagaaa
     6961 cagttgaagt gttgttagca cctttagctg ctaagtaacc tttaactgca tctgcgcgac
     7021 gttgacctaa tgcgatgttg tactctggag taccacgctc atcagcgtga ccagcaatag
     7081 ttacgttacc ttgagttttt gctaataatt gagcgtgagc gtctaataat tgacggtatt
     7141 caccttcaac tgcatagctg tcaaaaccga agtatacagt gttgtattgt gcttttaatt
     7201 gttcagctgt taaaccaccg aattcgcctg ctgcatttgc tgctgcattt gctgctgcgt
     7261 ttgctgctgc gtttgctgct gcatcatctg atgagctgct acatgctgct aatgcgaaag
     7321 ctggtactgc taccattaat actttagcaa attttttcat ttgttgctcc taatagagaa
     7381 atattattat ttagttaagt atggtgacca tgcagggaat ttataatgtc cacctgcgcc
     7441 aggcaaatta gctttaaaac gcccatctgc agacaccaat tgtagcactt tactcgtacc
     7501 tttggtagag ctataaataa ccataatgcc attcggtgaa atacttggac tttcgtctaa
     7561 gaaagttgaa ctcagtactt ctgtgccacc tgaagcgaga tcgcgtttca caactctatc
     7621 accggcaatc atgattaaat tcttaccatc agatgatact ttcgcattat agctaccgct
     7681 accgcccatt gggctagcac caccacctga tgaactcatt ctataaactt gaggagaacc
     7741 tgcacggtca gacgtaaagt agattgtgct accatctgga gaccagcttg gttcagtatt
     7801 atttccagca ttagcggtca atttacttgg tgtaccaccg ccactaccta caacataaat
     7861 atttaactca ccatcttgat ttgaggcaaa cgcaatgcga gaaccgtctg gagagaatga
     7921 tggcgcacca ttatgtcctt tcaatgctgc cactacacgg cgagcacctg aacgaatatc
     7981 gtgaacaaca acctgcgctt ttttattttc aaatgtcaca taagctaatc tgctaccatc
     8041 tggtgaccac tctggcgaca ttaaaggctc tttactggtt gtaatagtaa aggcattaaa
     8101 accatcataa tcagatactc gtaattcata cgatgatacg ccacgttgaa ccacatacgc
     8161 aattttagtt ctaaatgcac cgcgaatttg ggttaatttt tcaaatacct catcacttac
     8221 tgtatgcgca ccttgacgaa tttgggctgc cggaacaata aatgaacctt gggcaatgac
     8281 accacccgct gtacccgatg taccaagagt atcaattaat tgataagcga cattataacc
     8341 gccacctgtg ctactcactt ggccaactac aatattatca ataccgatag cactccattg
     8401 atctgccacc acttccgctg ctgatcccgg ttgagcaggc atttgagagc gttcaagcgg
     8461 tgtaaattta ccactatttc gcaaatcatc agaaataatt tgtgcaacat ctgccggcac
     8521 accgctggct ttaaatggca caaccgcaat cggctgagcc atacttacac cttcatcaat
     8581 agaaattcgc acatcagaat cagcaaccac ctgagttgtc aataatgctg aaaagatgcc
     8641 tattacaccc gatatacgag agaataattt catttgtagt cctctaaatt atttaacttt
     8701 taaactaaaa tcaagtgttg gtgatttaaa catattatat accgcatctg atggtgcagg
     8761 tggtactttt ctagttgcca ctattgcact taccgctgca tcacatactg ctttatcgcc
     8821 actgataact tgatagttag taattgtgcc atctcgactt aagaatatct taacatcaca
     8881 ttgttttccg gcaaaacttg gatcaacccg atatcgagat tgaattaatt tcttaattcg
     8941 ttgaccatat gcattaccat ctacattagt accttgccct aagccacttg taccaccaga
     9001 accttgtgat cctgctgagt ttttgttacc acctttactt gaaccaccac cgatgtctcc
     9061 actattgagg aaatcatcaa gtgctttatt gtttttcgct tgggcggctt ttgcctcagc
     9121 agctgccttc gctttagctt ctttctcggc tttctctttg gcttcttttt ctgcttttgc
     9181 tttggcttct ttctcagcct tttcttttgc ttctttttct gccttggctt tagcttcttt
     9241 ttctgctttt tccttcgctt ctttagcttc ttttgcctgc tgttccttca gttctttttg
     9301 ctcttttaac tttttctcag cttccgcttt cttctgctgt tcaagcttct tttcaagtgc
     9361 ttctttagct tttttctctt cttcagcttg tttagcagca gcctctaaac gcttagcttc
     9421 tgcatctgct ttcagttttg ctgcttcagc tgctttttta cgagttgctt cttctgcttg
     9481 ctgcttttta agctcttctt gcttagcttg ttcttgcttt tgctgttctt gtagtttttg
     9541 ctgacgctca atttcttgct gacgctgttt ctcttgaatc gccgctaact cagcttgttt
     9601 ctgtttttgg atctcttccg gagtcggttt atcttccaca tcttcttcaa cagcttcctc
     9661 tttaggctct acctttggct tcgatgcttt tgagcctttt ttctctgcca ataactgccc
     9721 atattcagca gccacttgcc cagtatctac catcacagct tcaaagctat cgctatcgcc
     9781 accactgcca ccagccgcag cgagttccgt cttagtgaaa agagacccga gcaataacaa
     9841 cccgattaac aatatatgca ataagatcga aacaataacc gctagctcta gtctatcttg
     9901 ttgagatttc acacgttatc cttggctatt taccttctgt cattaaacct accgatttca
     9961 ctccggcgtc tttaagtagt gtaatacctt tcataatttc ttcataaggt actactttat
    10021 caccggcaac taaaaataag gtattgttat ctttttgaaa ttgttcccct gcataggcaa
    10081 caacttctga ttcattaata ttctcttgcc cattactttg cacatagtta ccgtcaattt
    10141 tcaatttata ttcaccaaca cctgccacct ccaaaatcac tggagtttta tcttcattag
    10201 aaactgattg gctatgcgta tcttccggta aattaacttc tacactttga ctgataattg
    10261 gggccgttgc cataaaaatc agcaataata ccaacaatac atcaagaaat ggaacaatgt
    10321 tgatttctga tttaatgtca ttacgtttta aacgacgaga catatattcc tctttacagg
    10381 tgcttagcac cttaattgat ctgaaataac cggcacagtg tcccggtctc gaataatgcg
    10441 gttacaagcg gtctttttta cgaaaaactt tacaaatttt cattaaaaat caaccgcttg
    10501 tagattatct ttttgaaaaa gcctgacggt gtaataaggt cgtgaactca tcaatgaaat
    10561 tcacataccc ttgctcaact ttacctaaac gtaaattcaa acggttatat gccattactg
    10621 caggaatcgc cgcaaataaa ccgatagccg ttgcaattaa tgcttctgca atacccggtg
    10681 cgactgattg taaagttgct tgcttaactg cacttaatcc cataaaggag tgcataattc
    10741 cccaaaccgt tccgaataaa ccaatatatg ggctgattga acccactgtg cctaaaaacg
    10801 gaatgtagtt ttctaaactt tccatttcac ggtttaatgc taagttcatc gcacgggtag
    10861 aaccttgaat aatcgattct ggcgcatctg ggttcgcttg ttgtaaacgg gtaaattctt
    10921 taaacccaac ataaaagatt tgctctgaac catttaatgc atcacgacga ttctctaaac
    10981 cttggtgtaa acggtttaga tcttcccctg accagaatcg atcttcaaaa gcaagggaat
    11041 cttttttggc tttagttaaa attttgctgc gctgaataat caccgcccaa gacaaaattg
    11101 agaatacaat aaggatcagc atcacgatct ttaccacaat acttgcttct aaaaataacg
    11161 aaagaagact aaattcagtt tgcattaata aaactccgaa tagtttttaa atagaaaaat
    11221 aacttttttg taatgcttga cgaatatcat caggaattgc aaccggcttc attttgatta
    11281 aatcaacaca agcgaccgta acatttgcct cactcagaca atcgccatct ttccacaatc
    11341 gttgtttaaa gataagggtt gctttcttca attctgagag tgttgtttcc acatttaata
    11401 aatcatctaa acgagcaggg agcttataat ctatttcaat cctcttaaca acaaaagcaa
    11461 gggattggct aaaaagttcc tgctgtgaga aatgctgctg tctcaaaaat tccgttcttg
    11521 cccgctcaaa aaaattcaga tagttggaat gataaactac tccgccggca tcagtatctt
    11581 cataataaac acgaatcggg aaatccataa gcattatttc tctcattcaa aatgaaaaaa
    11641 cgcccaaaat ttaggcgcaa
//
LOCUS       DANLYI010000062        11388 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.062, whole genome shotgun sequence.
ACCESSION   DANLYI010000062 DANLYI010000000
VERSION     DANLYI010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 11388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11388
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.062"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            <1..2952
                     /gene="mscK"
                     /locus_tag="PXD81_001221"
     CDS             <1..2952
                     /gene="mscK"
                     /locus_tag="PXD81_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HDL5537248.1"
                     /translation="QNTAPDKAQTALTEMVKRKQEISSQLSATNIKNELQTKLEVELE
                     LIELKNTYNQILLGGSEDLSALYSAQLDEKKQEQQNLQAEIANLQTAINNKIVEESQN
                     KLDQAQAVQNQQNNATTNPVILRELDINTKVTQELLKQTKDMTQLSQDNLRIKSVLDN
                     LQQTQRNIEEQISSLQGTLVLSRIINKQKQSLPQDEMISGLSKQIADLRVRVFDITEF
                     KDSFADINAYISRIEQDEKTTFTSKEKEQLSKILQERSDTLTEMIKSLNNQLNLLINI
                     ELNQQQVQTISDALQQKLQQQSFWVKSNNPIDLEWFSKFVDITHYQFIDIGKKLNFSN
                     WRDNLLPASILVGILLLLAIFINRQKDKIKQKLHQINNRLKIVATDSQWNTPLAIFWT
                     LVLCLPSTLMFLAGFVAITYISFENPQEVWTWGLKMAIYWLYFAFLVAMLRPHGIGHI
                     HFNMPQKSNEIFRNILKRSVWIIALLLNTSIFTNLEMGISYDMLGQTMTIFVLILMIW
                     MIVPAFRQAISTYENVTTEEKKESKSILLTIVKLVLLLAPIILIILIALGYYYTALVI
                     IEHLVDSYFAIITWIIIRNILYRAFSISSRRLAYRRLQEKREAAEAKTRAKAEQNDEN
                     PDGVELPFELKEDSIAVSEVKNQMLKLTDFVLWIGLFVLLYWVWSDLITVALYLDGVT
                     LWQQSVTTEAGIVMESITLLNLLTAILIIVVMVVLIRNLSGLLEALIFSHFKLSQGTP
                     YTITTLLTYLIIALGAIFAFGTLGMSWSKLQWLLTALSVGLGFGMQEIFANFVSGIII
                     LFERPMRIGDTITIGTYSGTVSKIRIRATTLVDFDGKEVIVPNKAFVTERLVNWALSS
                     TATRIIVQVGVAYGSDLELVKRLLLQVAEENPKVLKDPQPRAYFLTFGASTLDHELRA
                     YVNDLSDRNPTIDALNTRIHQLFNEHNIDIAFNQLDVYIKNNDTQDEIKVDSKRFEFE
                     RPNNITAKEKE"
     gene            2955..4037
                     /gene="aroC"
                     /locus_tag="PXD81_001222"
     CDS             2955..4037
                     /gene="aroC"
                     /locus_tag="PXD81_001222"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012072711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HDL5537249.1"
                     /translation="MAGNSIGQLFKVTTFGESHGIALGCIVDGVPPNMALSEADLQPD
                     LDRRKPGTSRYTTPRREDDEVQILSGVFEGKTTGTSIGLIIKNGDQRSQDYGDIAEKF
                     RPGHADYTYQQKYGIRDYRGGGRSSARETAMRVAAGAIAKKYLREQFGIEVRGYLSQI
                     GNIKIDPKTVENIEQIDWHAVNSNPFFCPDPVAIEGFDALIRELKKEGDSVGAKLTVI
                     AERVPVGLGEPVFDRLDADLAHALMSINAVKAVEIGDGFAVVEQRGSQHRDEMTPQGF
                     LSNHAGGILGGISSGQPIIAHIALKPTSSITIPGRSVNKNNEAVEVITKGRHDPCVGI
                     RAVPIAEAMMAIVLLDHLLRFKAQCR"
     gene            4056..4883
                     /gene="mepA"
                     /locus_tag="PXD81_001223"
     CDS             4056..4883
                     /gene="mepA"
                     /locus_tag="PXD81_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HDL5537250.1"
                     /translation="MKLLKTLLVSSLAFTATALNATQWEKIKTPVQGKAQSIGGYSNG
                     CIIGAQPLALKGEGYQVIRSARNRYYGHPQLLDYLTHLGKKTKAAGVPTILIGDMGMP
                     AGGRFASGHASHQTGLDADIWFKFGPMDDETARNPAGLATIMVNRSTERVDDSLWNQH
                     HTNLVKLAATDPRVDRIFVNPAIKVKLCQTAGNDRAWLRKIRPWYGHDSHFHVRLTCP
                     ADAASCENQALVPAGDGCGEELYSWFEPKEASGTPKAKVLPTAPVQCQMILSEQGLN"
     gene            4956..6875
                     /locus_tag="PXD81_001224"
     CDS             4956..6875
                     /locus_tag="PXD81_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HDL5537251.1"
                     /translation="MRLFVAEKPSLARAIADVLPKPHTKGDGFIQCGEHDTVTWCIGH
                     LLEQAEPDVYDERFKMWRMEHLPIVPEQWKLIPKKETLKQFKVVEKLIKKADVLVNAG
                     DPDREGQLLVDEVFGYLNLSAERKSQIQRCLVSDLNPNAVSRAIEKLQKNSDFIPLAT
                     SALARARADWLYGINMTRAYTLQGRWAGYKGVLSVGRVQTPVLGLIVRRDLEIEHFVP
                     KDYFEVLAQIVVPETQERFKAQWQPSKACEDYQDEDGRVLSRPLAENVVKRITDQPAT
                     VTEYQNKIEKETAPLPYSLSVLQMEAAGRYGLSAQAVLDICQKLYETHKLITYPRSDN
                     RYLPTEHFNDRFKVMAAIAHHLPEYQQKPEVIDATIRNRCWNDKEVEAHHAIIPTARQ
                     GTVHLTENEKRVYQLIARQYLMQFCPDAEYRKSKIGLEIAGGSFVAQARNLVIAGWRA
                     LLGKEDSLDDAEPLLPIVKKGQILHCEKGEIVSKKTQPPRHFTDRSLLSAMTGIARFV
                     QDKELKKILRETDGLGTEATRAGIIELLFKRGFLIRKGKNIHSTEAGRILIQALPDSA
                     TQPDMTAHWEMQLNQISKKETSYQQFMFDLNQQLPHLLQSPNRQVLQNLAKITPLASG
                     NKPRYFKKRTQTAEN"
     gene            6981..7307
                     /gene="secG"
                     /locus_tag="PXD81_001225"
     CDS             6981..7307
                     /gene="secG"
                     /locus_tag="PXD81_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HDL5537252.1"
                     /translation="MLINVLTIVYLVVAVLLIAFVLMQQGKGADAGASFGAGASATVF
                     GSAGSANFLSRTTALLATIFFAISLVIGNLSSHNVAPKSQFEDLSQVEQKQVEPVKIE
                     NSDIPQ"
     gene            7332..7417
                     /locus_tag="PXD81_001226"
     tRNA            7332..7417
                     /locus_tag="PXD81_001226"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7366..7368,aa:Leu,seq:gag)
     gene            7620..8861
                     /locus_tag="PXD81_001227"
     CDS             7620..8861
                     /locus_tag="PXD81_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDL5537253.1"
                     /translation="MARTTKALNKTQIENAKSKDKEYILSDGDGLNLRVKPLPKGSKI
                     WIFNYTNPQTKNRTNLTIGRYPLITLAEARTICDGYRSLIEKGIDPQEEKKRLQQEAE
                     NRVNDTFRNVAESYFNGIYKEKAKNPITREKNWIRLENHIFPYIGDKHVEEIKVKALV
                     DIYSKVADKSNTLKKLHQLVSAVMDHAITQGIIESHNCRLAVKNFYIKPSTPNPTIEL
                     EELPQLFTDLHNAKLSKQTYALVCWSFLTALRPNEAVNAEWKEIDFEKKLWNIPKEKM
                     KGKADKKRPHSVPLSSQALKILEMMKMHSNSSPFVFPGRSSNSKPMSNATVNSTLKRN
                     GYKDRLTAHGIRAFVKTFLSSRKVDRHVSESILSHLLEGGDDLENTYNRYDFLEERVP
                     VMQLLGDYCEQCGMNLELVRG"
     gene            complement(9020..10888)
                     /locus_tag="PXD81_001228"
     CDS             complement(9020..10888)
                     /locus_tag="PXD81_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HDL5537254.1"
                     /translation="MFKKLLELRQQKAAIEAEMRSILTKADEEKRSTTEAEGAKFDEL
                     RSQAENLVKEIARYEAITDEQRNQPGQQVETANVKVSDDELRHWIKTGELRSLATNTN
                     GGADGGYSVIPELDKQVMKRLTDDSVMRQIANVVKLTQGKEYKKLVSAGGAAVNHIGE
                     GEARTETATPKMNEVTIAVHNIYAYPKTTQEILDFGGVDILGWLTEEISESFVETEET
                     DLTNGTGTKQAKGFLAYPRTTANDKTRPFGTLQKMEVQPATVIGWNSKGQPETLTPYP
                     PYSVNVQRTVNRQNWLTAYIPHSYDENVAETPHFDQWLNFVSDGKQDKAKNILAALYA
                     ILTNRYNWQVFFQVTGKGGSGKSVFAGIATLLAGEKNTASARLENFDDERKIAGLEDK
                     KLIICSEQTKYAGDGGGLKAITGGDMVRVDPKNKHPFNARIAAMIMIVNNDPCKWTER
                     NGGIDRRIVNFRFNKRPPENERDPYFMDKITLEIGGIIRKVLDTFPDPLEAKRALEEQ
                     KESLEALEIKKQSDPLTDFFEYLYTTENTDGLYIGTGNTLGHDKIRTHLYPAYLAFVK
                     AKNVLELGLNTFVVGIEQAVKQHGNKHDFTKRKTNKGQRTNVHFKNFDDFQSDILN"
     gene            complement(10899..11294)
                     /locus_tag="PXD81_001229"
     CDS             complement(10899..11294)
                     /locus_tag="PXD81_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL5537255.1"
                     /translation="MISNEAIRQEVIDLLQGNIQGIQHFHNGRAVFTDVENELPAIAV
                     FIDEAEYSQLTLCDGECNAYLKIGIYLPMFSTENDLDLVAQKISDVMNQATFHSMDEC
                     VLAKYSYDYDANESAWKTATLHYNINYLN"
BASE COUNT         3447 a         2380 c         2341 g         3220 t
ORIGIN      
        1 caaaataccg caccggataa ggctcaaacg gccttaaccg agatggttaa acgtaaacag
       61 gaaattagta gccaactttc agcgactaac attaaaaatg aactacagac aaagttagaa
      121 gtagaattag aattaataga attaaaaaac acttacaatc aaatattact tggtggcagc
      181 gaggatttat ctgcgcttta ctctgctcaa ttagatgaaa aaaagcaaga gcagcaaaat
      241 ctacaagcag aaattgcaaa cctgcagact gctatcaaca ataaaattgt agaagagtct
      301 cagaacaagc tagatcaagc acaagcggtt caaaatcagc aaaataatgc aacgacgaac
      361 ccagttatct tgcgtgaatt ggatataaat accaaagtca cacaagagtt gctaaaacag
      421 accaaagata tgacgcagtt atcacaagat aatttgcgaa tcaaaagtgt gctagataac
      481 ttacagcaaa cccaacgtaa tattgaagaa caaatcagtt ctctgcaagg tacgttggtg
      541 ctttctcgta ttattaataa gcaaaaacaa tcattaccac aagatgaaat gatttccgga
      601 ctatcaaaac aaattgcaga tttacgtgta cgtgtttttg atatcactga atttaaagac
      661 tcttttgcgg acataaatgc ttatatcagc cgcatagagc aagatgaaaa aacaaccttt
      721 acgagtaaag agaaagaaca actgagtaaa attttacagg aacgttctga cactttaact
      781 gagatgatta aatcattaaa taatcagctc aatttattaa tcaatattga actaaaccag
      841 caacaagtgc agaccattag tgatgcgtta cagcaaaaac tgcaacagca aagcttctgg
      901 gttaaaagta acaaccccat tgatcttgaa tggtttagta aatttgtcga tattacccac
      961 tatcaattta ttgatattgg aaaaaaactc aatttttcaa attggcggga taatttatta
     1021 cctgcaagta ttttagtcgg aattttattg ctattagcca tctttattaa tcgccaaaaa
     1081 gataaaatta aacaaaaatt acaccaaatt aataatcgac taaaaattgt cgccacagat
     1141 agccaatgga acacccctct tgctattttt tggacattag tgctttgcct gccaagtaca
     1201 ctaatgttct tagccggatt tgttgctatc acttatattt catttgaaaa tcctcaggaa
     1261 gtctggactt ggggattaaa aatggcaatt tattggctgt attttgcctt tttagtggcg
     1321 atgctacgcc cgcacggcat aggacatatt cactttaata tgccacaaaa aagtaatgaa
     1381 atcttccgta atattttaaa acgttcggta tggatcattg ctttattact gaacacctct
     1441 atttttacaa acttagagat gggaatatct tacgacatgc ttggacaaac aatgactatt
     1501 tttgtcctta tattaatgat ttggatgatt gtaccggcat ttaggcaagc tatttctacc
     1561 tatgagaatg tcacaacaga agagaaaaaa gaatctaaaa gtattttact cactattgtc
     1621 aaattagtgc tactgcttgc ccctatcatt cttatcattc tgattgcatt gggctattac
     1681 tatactgcac tggtgatcat tgagcattta gtagattctt attttgcgat cattacttgg
     1741 attattattc gtaatattct ctatcgagca ttctctattt catcacgccg tctggcttac
     1801 agacgtttac aagaaaaaag agaagcagct gaagccaaaa cacgagctaa agcagaacaa
     1861 aatgatgaaa acccggacgg tgtcgagctt ccttttgagc taaaagaaga ctctattgcc
     1921 gtaagcgaag tcaagaacca aatgttgaaa ttaactgatt ttgtgctttg gattggctta
     1981 tttgtgctgc tttactgggt ttggtcggac ttaattactg ttgcactcta cctagacggc
     2041 gttaccttat ggcaacaaag tgttacaaca gaagccggga ttgtaatgga atccattacc
     2101 ctgctgaatt tactcactgc tattctgatt attgtggtaa tggtggtact cattcggaac
     2161 ttatccggtt tacttgaagc cctgattttc tcgcacttca agttatcgca aggtacacca
     2221 tacacaatta ccacactctt aacctatttg attattgcgt taggggcaat ctttgcgttc
     2281 ggtacattag ggatgtcttg gagcaagctc caatggctac taactgcact ttctgtcggt
     2341 ctgggttttg gtatgcaaga gatctttgca aacttcgttt cagggattat catcttgttt
     2401 gaacgtccaa tgcggattgg cgacaccatt accatcggca cttacagcgg tacggtttcc
     2461 aaaattcgta ttcgtgccac aaccttagtc gattttgacg gcaaagaagt gattgtacca
     2521 aacaaagcat tcgtaaccga acgcttggtg aactgggcat taagcagcac cgcaacccgc
     2581 attattgttc aagtcggcgt ggcttacggg tctgatttag aattagtcaa acggctgtta
     2641 ttacaagtgg cggaagaaaa tccgaaagtc ttgaaagatc cacaaccaag ggcttatttc
     2701 cttacctttg gtgcaagcac tttagatcac gaattaagag cctatgtgaa cgatttatct
     2761 gatcgtaacc caactattga tgcactaaat acacgcatcc accagctctt taatgaacat
     2821 aatattgata ttgcatttaa ccaattagat gtttatatca aaaacaacga tacacaagat
     2881 gaaattaaag tagattcaaa acgctttgag tttgaacgac caaataacat aacagcaaaa
     2941 gaaaaggaat aacaatggca ggcaacagca tcggacaatt atttaaagtg accactttcg
     3001 gcgaatctca cggtattgca ttaggctgta tcgtagatgg cgtaccgccg aatatggctt
     3061 taagcgaagc agatttacag ccggatttag accgccgtaa acccggtact tcacgctaca
     3121 ccacgcctcg ccgtgaagat gatgaagtgc aaattttatc gggagtcttt gaaggcaaaa
     3181 cgacgggaac aagcattggt ttaatcatta aaaacggtga ccaacgctca caagactatg
     3241 gcgacattgc cgaaaaattc cgcccggggc acgcagatta tacctatcag caaaaatacg
     3301 gcattcgaga ttaccgtggc ggtgggcgtt cttctgctcg ggaaaccgca atgcgggttg
     3361 ccgccggggc gattgccaaa aaatatttgc gtgagcaatt tggcattgaa gtgcgaggct
     3421 acctctcaca aatcggcaat atcaaaattg acccgaaaac cgttgaaaat attgaacaaa
     3481 ttgattggca cgcagtcaat agtaatcctt tcttctgccc cgatcccgtt gcgattgaag
     3541 gctttgatgc cttaattcgt gagctgaaaa aagagggcga ttcggtgggg gcaaaattaa
     3601 ccgtgattgc agagcgtgta cctgtagggt taggcgaacc tgtgtttgac cgcctagatg
     3661 ccgaccttgc ccacgcctta atgtcgatca acgccgtgaa agcggtggaa attggcgatg
     3721 gttttgcagt tgtggaacaa cgtggtagcc agcaccgaga tgagatgacg ccgcaaggct
     3781 ttctctctaa tcacgccggc ggaattttag gtggtattag ctccggtcag ccgattatcg
     3841 ctcatattgc gttaaagccg acctcaagca ttactattcc cggcagaagc gtgaacaaaa
     3901 acaacgaagc cgttgaagtg atcactaaag gcagacacga cccctgtgta ggtattcgtg
     3961 ctgtgccgat tgcagaagcg atgatggcga ttgtactact cgaccatctg ttaagattta
     4021 aggctcaatg ccgttaaaca cataaaattg gaaaaatgaa attattaaaa acacttttag
     4081 tctcaagcct tgcttttaca gctaccgcac tgaatgcaac gcaatgggag aaaatcaaaa
     4141 ctccggttca aggaaaagca caatctatcg gcggttatag taacggctgt attattgggg
     4201 cacagcccct tgcattaaaa ggcgaaggct accaagtgat ccgctcggct cgtaaccgct
     4261 attacggaca cccacaactg ctcgattatc tcactcactt aggcaaaaaa accaaagctg
     4321 ccggtgtgcc gaccatttta atcggcgata tgggaatgcc tgccggtggg cgttttgcct
     4381 ccggtcacgc ctcccatcaa accgggctgg atgctgatat ttggtttaag ttcggaccga
     4441 tggacgatga aaccgcccgc aatccagcag ggcttgccac cattatggta aaccgtagta
     4501 ccgagcgggt tgatgattca ctctggaatc aacaccatac taatttagta aaacttgctg
     4561 caaccgatcc tcgagtggat cgcatttttg tcaatcctgc cattaaagtg aaactctgcc
     4621 aaaccgcagg caatgatcgt gcttggttac gcaaaattcg cccgtggtac ggacacgact
     4681 cccatttcca cgtccgttta acctgcccgg ctgatgccgc aagttgtgaa aatcaagccc
     4741 ttgtgcctgc cggtgacggc tgtggtgaag agctttactc gtggtttgag ccgaaagaag
     4801 cctccggcac accaaaagcc aaagtgctac ccacagcgcc tgttcaatgt cagatgattt
     4861 tatctgaaca aggcttgaat taattgcaaa ataacaacct tttttgaccg cttgtcagcc
     4921 cacaagcggt tcattttctt aaataattta cgattatgcg tttatttgtt gccgaaaaac
     4981 ccagccttgc ccgagcgatt gccgatgtgt taccaaaacc ccataccaaa ggagatggct
     5041 ttatccaatg cggcgaacac gacactgtta cttggtgtat cggtcattta ttggaacagg
     5101 ctgaacccga tgtctatgat gagcgtttca aaatgtggcg aatggaacat ttgccgattg
     5161 tgcctgaaca atggaaattg attcccaaaa aagagacgct caagcagttt aaagtggtgg
     5221 aaaagctgat taaaaaagcc gatgttttag tcaatgccgg agacccggat cgagaagggc
     5281 agctgttggt ggacgaagtt tttggctatt tgaatttatc tgctgaacgc aaaagccaaa
     5341 ttcagcgttg tttggtaagc gatctcaacc cgaatgcggt gagccgagcc attgaaaaat
     5401 tgcaaaaaaa cagcgatttt ataccgcttg caacctctgc tctcgcccga gccagagcgg
     5461 attggctgta tggtatcaat atgacccgag cctataccct acaggggcgt tgggcaggct
     5521 ataaaggcgt gttatcagtc ggacgtgtgc aaacgccggt attagggctg attgttcgcc
     5581 gagatctgga gatcgagcat ttcgtgccaa aggattattt tgaggtgttg gcacagattg
     5641 tcgtgccgga aacccaagag cgatttaagg cgcagtggca accgagcaaa gcctgtgaag
     5701 attatcaaga tgaagatggg cgggtgctgt ctcgtccatt ggcagaaaat gtggtaaaac
     5761 ggattaccga ccagccggca accgtcaccg aatatcaaaa taaaattgaa aaagaaactg
     5821 caccgctgcc ttattctctt tcggtattac aaatggaagc cgccggcaga tatgggctat
     5881 ccgcccaagc ggtgttggat atttgccaaa agctgtatga aacccataaa ctaattacct
     5941 accctcgttc tgataatcgg tatttgccga ccgaacattt caacgacaga tttaaagtga
     6001 tggcggcgat tgctcatcat ttacctgaat atcaacaaaa accggaagtg attgatgcca
     6061 ctattcgcaa tcgttgctgg aatgataaag aggtggaagc ccaccacgcc attattccca
     6121 ctgcacggca agggacggta catttaaccg aaaatgaaaa aagagtttat caactcattg
     6181 cccgccaata tctaatgcaa ttctgccctg atgcggaata tcgcaaaagt aaaatcgggc
     6241 ttgaaattgc aggtggtagt tttgtcgctc aagcacgcaa ccttgtaatt gccggctggc
     6301 gagcgttact gggtaaagaa gatagccttg acgatgcaga gccactacta ccgattgtca
     6361 aaaaagggca aatcctccat tgtgaaaaag gggaaattgt cagcaaaaaa acacaaccgc
     6421 cccgccactt tactgatcgc agtttgcttt cagcaatgac cgggatcgcc cgttttgtac
     6481 aagataaaga attgaaaaaa atcttgcgtg aaaccgatgg tttaggcacg gaggcaaccc
     6541 gagccggtat tatcgaattg ctgtttaaac gtggtttttt aatccgtaaa gggaaaaata
     6601 tccacagtac cgaagccggc agaatattga ttcaagcact tcccgactct gctacccagc
     6661 cggatatgac cgctcattgg gaaatgcagc tgaatcaaat cagcaaaaaa gagaccagct
     6721 atcagcaatt tatgtttgat ctaaaccaac agctcccaca tttattacaa agcccaaacc
     6781 gccaagtgtt gcaaaattta gcgaaaatca caccgcttgc aagcggcaat aaacctcgtt
     6841 attttaaaaa gcgaacacaa actgctgaaa attaaatcaa gcaggtaaaa attactgttt
     6901 agcacttgtc aaacgggcgt aaattctata ttatgcacgc cattgcaaat ttcgcataaa
     6961 gaatacaaga gaataaatta atgttaatta atgtactcac tattgtttat cttgtagttg
     7021 ccgtattact gatcgcattt gtattaatgc aacaaggtaa aggcgctgat gcaggtgctt
     7081 ctttcggggc gggtgcttca gcaacagttt tcggctcagc aggatcagca aattttttat
     7141 caagaacaac tgctctactt gcaacgattt tcttcgcaat tagtttagtt atcggtaatt
     7201 taagctcaca caatgtagca ccgaaaagcc aatttgaaga cttaagccaa gttgaacaaa
     7261 agcaagtaga acctgtaaaa atagaaaata gcgatatccc tcaataatcg cttaacatta
     7321 aaaagaataa agctctggtg gtggaattgg tagacacgcc atcttgaggg ggtggtggac
     7381 acagtccgtg cgagttcaag tctcgcccag agcaccattc aactaaattt ggctcaacgg
     7441 agctaaacta aaaaagcctt agaaagcctg taaaatcaac atttacaggc ttttttacta
     7501 tcttaactca acggctctaa acgtttccta tcacaaattt tagttacacg tttagttaca
     7561 cgttttaaat cgtgttacta tactttcaat aatcgtgtaa cacttcgtag gtttaattta
     7621 tggctagaac aactaaagca ttaaataaaa ctcaaataga aaatgccaaa tcaaaggata
     7681 aagaatatat tttatccgat ggcgatggat taaatcttag agttaaacct ctccctaaag
     7741 gctctaaaat ttggattttt aactacacta acccacaaac taaaaacaga acaaatttaa
     7801 ctattgggcg ttacccctta attacacttg cggaagctcg cactatatgt gatggctacc
     7861 gctcgttaat agagaaaggc atagatccac aggaagaaaa aaagcggctg caacaagaag
     7921 cagaaaatcg agtaaatgat actttccgga atgtggccga aagctatttc aatggtattt
     7981 ataaagagaa agcaaaaaat ccgattactc gtgaaaagaa ttggataagg ttagaaaatc
     8041 atattttccc ctatatcggg gataaacacg ttgaagaaat caaagtaaaa gcccttgttg
     8101 atatttattc taaagtggca gataaaagca acacattaaa aaaactgcat cagcttgtat
     8161 ctgctgtaat ggatcacgcc attacgcaag gcattataga aagccataac tgccgattag
     8221 cagttaaaaa cttttatatc aagccatcta cgccaaatcc cactattgag cttgaagaat
     8281 taccgcagtt atttactgat ttacataatg cgaagttatc aaagcaaact tacgcccttg
     8341 tttgttggtc gttcttgact gctctacgcc caaatgaagc cgttaatgct gaatggaaag
     8401 aaatagactt tgagaaaaag ctatggaata tccctaaaga gaagatgaaa ggaaaagcag
     8461 ataaaaaacg ccctcactct gtgccgttat cctcccaagc gttaaagata ttagagatga
     8521 tgaaaatgca ctctaattct agcccttttg tttttccagg tcgttcatca aatagtaaac
     8581 caatgagcaa cgcaacggta aattcaacat taaaaaggaa tggatataaa gatagactaa
     8641 ccgcacacgg tattcgggca tttgtgaaaa catttctatc ttctcgcaaa gtagatcgcc
     8701 acgtttccga aagtatatta tcccatttat tagaaggtgg agatgattta gaaaacacat
     8761 acaaccgata tgatttttta gaagagcgtg tgccagtaat gcagctttta ggtgattatt
     8821 gcgaacagtg cggaatgaat ttagagttag ttcggggtta gggaggtaaa atatatgatt
     8881 ttagtcgtca cgtcatcact tttcccactt tcccctttaa aatcaaatag ttaagtggtg
     8941 atgactggtt ttaaagtcat cattaagtca tcacaaaaag gtgatgacct agaaaaaaag
     9001 aggggtttcc ccctctttgc taattcaaaa tatcactttg aaagtcatca aaatttttga
     9061 aatgaacgtt tgttcgttgc cctttattcg tctttcgttt tgtgaaatcg tgcttattgc
     9121 cgtgttgctt tactgcttgc tcaataccta ctacaaaagt atttaagcct agttctagca
     9181 cattcttagc cttaacaaag gctaaatatg ccggataaag gtgcgttctt attttgtcgt
     9241 gtccaagcgt gttgccagtt ccgatataaa gcccgtctgt attttctgtt gtatatagat
     9301 actcgaaaaa atccgttaat gggtcagact gctttttaat ctccaacgcc tctaggctct
     9361 ctttttgctc ctctaaggct cttttagctt cgagtgggtc agggaaggta tcaagcactt
     9421 tacggataat gccccctatc tctaatgtaa ttttatccat aaaatacgga tcacgttcat
     9481 tttcaggcgg tcttttatta aaacggaaat tgacaatccg cctatctata ccgccattcc
     9541 gttctgtcca tttacaaggg tcgttattga cgatcataat cattgccgca attcttgcgt
     9601 taaatgggtg cttgttcttc ggatcgactc gcaccatatc accgccagta atcgctttca
     9661 atccgccacc atctccagcg tatttcgttt gctcggaaca gataatcagc tttttatctt
     9721 ccagccctgc gattttgcgt tcatcatcaa agttttctag ccttgctgaa gctgtgttct
     9781 tttctccagc taatagtgtc gcaatgccag caaataccga tttaccgctt ccgcctttcc
     9841 ctgttacttg gaagaatact tgccagttgt agcggtttgt taaaatcgca tagagagcgg
     9901 ctagaatgtt cttcgccttg tcttgcttgc cgtctgatac aaagttaagc cattgatcaa
     9961 agtgtggtgt ttcggctacg ttctcgtcat agctatgtgg aatataagcc gttagccagt
    10021 tttggcggtt aacggttctt tgaacattta ccgaataagg tgggtaaggg gttaaagttt
    10081 cgggttgccc cttgctattc caaccaatca ccgtagccgg ttgtacttcc attttttgaa
    10141 gcgtgccgaa tggtcgggtt ttatcgttgg ctgtcgtgcg tggataagct aagaagcctt
    10201 tagcttgttt tgtgcctgta ccgttggtta aatcggtttc ttcagtttcg acaaaggatt
    10261 ctgaaatttc ttcagtcagc cagcctagaa tatctacacc accaaagtct aaaatctctt
    10321 gcgtggtttt cgggtaagcg taaatattat gaacggcaat cgttacttca ttcattttcg
    10381 gggtggcggt ttcggtgcgt gcttcgcctt caccgatatg atttaccgct gcaccgccag
    10441 ccgatactaa ttttttatat tctttgcctt gggtcagttt caccacgtta gcaatttgtc
    10501 gcataacaga atcatctgtt aggcgtttca ttacttgctt gtcaagttcc ggaatcactg
    10561 aatagccacc gtctgcccct ccatttgtat tcgtagcaag cgaacgcaat tcgcctgttt
    10621 taatccagtg gcgaagctcg tcatcagata ctttcacatt tgcggtttca acctgttgcc
    10681 ccggttggtt acgctgttca tcagtaatcg cttcataacg tgcgatttct tttactaaat
    10741 tttcagcttg tgagcggagt tcatcaaact tagccccttc tgcttcagtt gttgagcgtt
    10801 tttcttcgtc tgctttggtt aaaattgaac gcatttctgc ttcaatagcc gctttttgtt
    10861 ggcggagttc taaaagtttt ttaaacattg ccttatcctt aattcaaata attgatattg
    10921 tagtgaagcg ttgctgtttt ccacgctgat tcgttagcat cataatcata gctatactta
    10981 gccagcacac attcgtccat actgtggaat gtggcttgat tcatcacatc actgattttt
    11041 tgagccacta aatctaggtc gttttcggtt gaaaacattg gtaagtaaat gccgattttc
    11101 aaataggcgt tgcattcgcc atcgcaaagc gtgagttggc tatattccgc ttcatcaatg
    11161 aatacggcga tagccggtaa ttcattttct acatcggtga atacggctcg gccgttatgg
    11221 aaatgttgaa tgccttgaat gttaccttgt aataagtcga taacttcttg gcggattgct
    11281 tcgttagaaa tcataaaaaa gtcctcttaa ttaagaaggc tacaattagc cacagaacaa
    11341 tataggcata atttcaacgg agtaaatagg gtaaaattta atcgttta
//
LOCUS       DANLYI010000063        11296 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.063, whole genome shotgun sequence.
ACCESSION   DANLYI010000063 DANLYI010000000
VERSION     DANLYI010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 11296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11296
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.063"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            76..975
                     /gene="corC"
                     /locus_tag="PXD81_001230"
     CDS             76..975
                     /gene="corC"
                     /locus_tag="PXD81_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249295.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HDL5537256.1"
                     /translation="MSDEQQSVNTTEKSEKKSILHSLFSGLFQNEPKNREDLVEVIRD
                     SVENELIDSDTKEMIEGVMEISELRVRDIMIPRAQIVFIDANEPLDVCIDLIIESAHS
                     RFPVIRGEKDTIEGILHSKDLLKYLRSDSEPFNLQQVLRPAVIVPESKRLDRMLKEFR
                     SERFHMAVVVDEFGAVSGLVTIEDILEQIVGDIEDEFDEEEVKPIRQLSRHTYAVLPL
                     TDIKKFNEFFATDFNDEEVDTIGGLVMQAFGYFPKRDEQIELNGIDFKVTSANSRQLI
                     QLRVTLSDEQLEKMEKTSPNEEE"
     gene            1034..2542
                     /gene="lnt"
                     /locus_tag="PXD81_001231"
     CDS             1034..2542
                     /gene="lnt"
                     /locus_tag="PXD81_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005611523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HDL5537257.1"
                     /translation="MNFAKNPPFLTACLLAIILGAIGTFAYSPFDIWILAFASAAGLV
                     WSATLANRKTALWATFAWSIGYFCTGVNWVSVSMTQFGGVPTIVSYIAVFLLACYLAI
                     YNLLFSVVSHRLKLNNPFALAAIFTFTEYLRGVVFTGFPWLQFGYSLIDSPFAGIAPI
                     LGVEGLTFLVIVASSYLVKIIQQENKTVPSLMVLAVIFGLSFATRFLQFVQIDTEKQP
                     LVVSLVQGNIEQKMKWDPQHFNHSVRTYEQLILPLLGNSELIILPESAIPALENQISP
                     LLGSLDHLARQHGSEIIIGTLYQNEQEELFNSSVLLGEKEREYALHSNPRYNKHHLVP
                     FGEYVPFGSVLDWMREVFILPINLSQGSFVQPSFLAKNNRFNMAICYEIIFGDQLQQN
                     QKSQNSDYLLTITNDAWFGSSIGPWQHFQMARMRALELGKPLLRAANTGITAIVDANG
                     KVIEQLPQFTADALTATIVPTKGDTPFKQLGHWLIYSFSLFCVIASLLLRRK"
     gene            complement(2588..3052)
                     /locus_tag="PXD81_001232"
     CDS             complement(2588..3052)
                     /locus_tag="PXD81_001232"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HDL5537258.1"
                     /translation="MATFQGNYIATGLKFGIVATNWHKIFIDQLLNGATDKLLRHGVE
                     EKNIDTVWVPGALEISIAAKKMAQSGRYDAIICLGAVVRGATSHYDVVVNESAKGISS
                     SALETGVPIINGILTVENLEQAIERSGTKAGNKGEECAMVAIEMANLLKALA"
     gene            complement(3258..4457)
                     /locus_tag="PXD81_001233"
     CDS             complement(3258..4457)
                     /locus_tag="PXD81_001233"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HDL5537259.1"
                     /translation="MFKFSTVEEAIQAIAQGKIILVSDDADRENEGDFICAAEFATPE
                     NINFMAKYGKGLICTPISTEIAKKLNFHPMVLENEDNHQTAFTVSVDHIQTGTGISAF
                     ERSLTCLKMLDENAKPEDFRRPGHVFPLVAKDGGVLVRNGHTEATVDLARLAGLKPAG
                     LCCEIMAEDGTMMQMPELQAFAQAHNMPFITIQQLQEYRRKHDSLVNVVSVVKMPTKY
                     GEFKAHSFVETISGKEHVALVKGDIGDGENVLCRIHSECLTGDAFGSQRCDCGQQFAA
                     AMNQVEQEGRGVVLYLRQEGRGIGLINKLRAYELQDRGMDTVEANLALGFKDDEREYY
                     IAAQMFEKLGVKSIRLLTNNPAKIEGLIEQGLNVVAREPIMVEPNEHDLEYLKVKQHK
                     MRHMFNF"
     gene            complement(4476..5123)
                     /gene="ribE"
                     /locus_tag="PXD81_001234"
     CDS             complement(4476..5123)
                     /gene="ribE"
                     /locus_tag="PXD81_001234"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HDL5537260.1"
                     /translation="MFTGIIEEVGQIAQIKKQGEFVVVTVKAKKILTDVHLGDSIAVN
                     GVCLTVTSFTQEQFTADVMSETLKRTSLGELSINSSVNLERAMAANGRFGGHIVSGHI
                     DGTGVVAEITPADNSTWYRIKAEPKLMRYIIEKGSITIDGISLTVVDVDAESFRVSII
                     PHTIKETNLGTKKIGSLVNLENDIVGKYIEQFLLKKEPENPQSKITADFLKNAGF"
     gene            complement(5340..6437)
                     /gene="ribD"
                     /locus_tag="PXD81_001235"
     CDS             complement(5340..6437)
                     /gene="ribD"
                     /locus_tag="PXD81_001235"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005708727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HDL5537261.1"
                     /translation="MTDVQYMAYAITLAEKARGWTSPNPLVGCVIVKNGNIIAEGYHQ
                     KAGSWHAERNAILNCEEDLTGATAYVTLEPCCHHGRTPPCSELLIERGIKKVFIGSRD
                     PNPLVSGKGAAQLRAAGIEVVEDFMRTECDALNPIFFHYIQTKRPYVLLKYGMTADGK
                     IATYTGNSKWITGEAARANVQKSRHEYSAIMVGVETVLADDPMLNSRMENAKQPVRIV
                     CDSQLRTPLDSQLVQTARQYRTIIATLNNDAKKQAEYTACGVEILVTGERDKRVDLAD
                     LLQKLGELGIDSLLIEGGSQLNFSVLEQGLVNRVHCYIAPKLIGGKAAKTPIGGKGIG
                     ELAQAVKLRLKSTELLGEDILLDYEVDLLRN"
     regulatory      complement(6613..6731)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            6935..9337
                     /gene="lon"
                     /locus_tag="PXD81_001236"
     CDS             6935..9337
                     /gene="lon"
                     /locus_tag="PXD81_001236"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005623665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HDL5537262.1"
                     /translation="MAPRKKKAIELPLLPLRDVVVFPYMVMPLFVGREKSIQALRSAM
                     DSNKQLFLVTQQDPNKEEPNAEDMYGVGVIANIIQMLNLPDGTVKVLVEGQTRAKIEQ
                     IHDDENGFWAAIQPIYSEYDDENEELKAIAKTTLTEFENYVKNNKKIPAEIIAKLQKI
                     TLEDRLADTIASNLIAPVKKKQELLEQPNLIARFEALLIAMATEMDTLETETRIRNRV
                     KQQMEKNQRDYYLNEQIKAIQKELGNGDDVEQSELDKLKEKIDAAKLPTEVKDKLNTE
                     FKKLKAMPQSSSEATVVRGYIDWILQMPWHKKSAVKKDLAQAQEILDKDHYGLERVKE
                     RIVEYLAVQSRLNKLKGPILCLVGPPGVGKTSLGQSIANATGRKYVRMALGGVRDEAE
                     IRGHRRTYIGSMPGSLMMKMAKVGVRNPLFLLDEIDKMAQDMRGDPASALLEVLDPEQ
                     NKAFNDHYLEVDYDLSDVMFVATSNSMNIPPALLDRMEVIRLSGYTEDEKMHIARGHL
                     LAKQQENNGLKEGELTIEDSAILSIIRYYTREAGVRSLEREIAKICRKAVKALVLDKK
                     LKSITVSEKNIEEYLGVKRFDYGKMDSQNRIGEVTGLAWTEVGGDLLTIETASVAGKG
                     KFSYTGSLGDVMKESIQAAMVVVRSRAEKLGIAEDFHEKRDIHVHVPDGATPKDGPSA
                     GIAMCTALISSLTGNPVRKEVAMTGEISLRGKVLPIGGLKEKLLAAHRGGITTVIIPK
                     ENEKDLEEIPENAKAALDIHAVETIDEVLALALENPPMGIEILSKAEIKVKKSRAKAT
                     VQ"
     gene            complement(9471..10361)
                     /gene="ilvY"
                     /locus_tag="PXD81_001237"
     CDS             complement(9471..10361)
                     /gene="ilvY"
                     /locus_tag="PXD81_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HDL5537263.1"
                     /translation="MDFQSLKLFLDIAQSRSFIRSAEKNYMTASTLTRHIQRIEEELG
                     QPLFLRDNRQVRLTEAGERFLAFAQQGWAEWQQLQNQLSPAQGDLEGELKVFCSVTAS
                     YSHLPPILGKFRQKYPKVDIKLSTGDPALALEQVQSLAADISVAGKPEFLPNNIVFHY
                     IDDIQLSIIAPRVASQATQFLQQSPIDWKNIPFILPVNGPVRKRIDRWFKEQKIKDPI
                     IYATVSGHEAIVPMVALGFGVALLPDIVIKHSPMNNQVSYVNLPSPITPFELGICVQQ
                     KRLQEPIINAFWQLLSDNSA"
     gene            complement(10363..11100)
                     /locus_tag="PXD81_001238"
     CDS             complement(10363..11100)
                     /locus_tag="PXD81_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009020527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HDL5537264.1"
                     /translation="MRGGKQLAKEQYDVVIDPTVTLRNRDLLFLRTISAKHYVGYQKQ
                     KYRLFDLNVEDRKQHFSEIYQDALALIGFENIETQYDVPLNATSNQSIKHYLYENQIR
                     NYIALNLFGAGSARRFSEQKINELLSCLKQHSDKPIVLLTFPEVTAKLCSFTKKYKNI
                     FVYEKTKSVFDSIELIRFADLLISPDTATVHIASGLSKKIIAFYSSDEQNFIHWHPNN
                     QAETHILRFRKSVNDLDFAAIKPEWLK"
BASE COUNT         3266 a         2439 c         2305 g         3286 t
ORIGIN      
        1 aaaatagcaa tgttttattt gctttgtgac tgaatttgct tattataagc gtactttatt
       61 taagtggaac aaactatgag tgatgaacag caaagtgtaa acaccaccga aaaatcagaa
      121 aaaaaatcga ttttacactc tctatttagc gggctttttc aaaacgagcc taaaaaccgt
      181 gaagatctag tcgaagtgat tcgagactct gttgaaaatg aattgatcga cagcgacacc
      241 aaagagatga tcgaaggagt aatggaaatc tcggaattac gagtgcggga tattatgatt
      301 ccccgtgcgc aaattgtgtt tattgatgcc aatgaacctt tagatgtctg tattgattta
      361 attattgaat ccgcacactc tcgttttccg gtgattcggg gtgagaaaga tactatcgaa
      421 ggtattcttc actctaaaga tttattgaaa tatttacgtt ctgactctga accctttaat
      481 ctgcaacagg tattacgccc tgcggttatt gtaccggaaa gcaaacgcct agaccgaatg
      541 ctaaaagagt tccgttcaga acgcttccat atggcagtag tggttgatga atttggtgcg
      601 gtttcaggct tagtcaccat tgaagacatt cttgaacaaa ttgtgggtga tattgaagat
      661 gagtttgatg aagaagaggt caaaccaatt cgccagcttt caagacatac ttatgcggta
      721 ttaccgctca ccgacatcaa aaaattcaat gaattttttg ctaccgactt taatgacgaa
      781 gaggtggata ccattggtgg tttagtgatg caggctttcg gttatttccc gaaacgagat
      841 gagcaaattg agttaaacgg aattgatttt aaagtcacct cagctaatag ccgccaattg
      901 attcagctac gagtcacact ttccgatgaa cagttagaga aaatggaaaa aacctctcct
      961 aacgaagaag aataatatag tgagtttcaa tcaaggcacg tttcggcgtg cctcatttat
     1021 ttagtgagtt attatgaatt ttgcaaaaaa tccgcctttt ttaaccgctt gtctccttgc
     1081 aatcatactg ggtgcaatcg gcactttcgc ctattcgcct tttgatattt ggattcttgc
     1141 ctttgcttcc gcagccggct tggtttggtc agccactctg gcaaacagaa aaaccgcatt
     1201 atgggcaacc tttgcttggt ctatcggcta cttctgcacc ggtgtaaact gggtttctgt
     1261 gagtatgacc caatttggtg gtgttcctac catagtaagc tacatagcag tattcctgtt
     1321 ggcgtgctat ctcgctattt ataatctgct gtttagtgta gtttcacaca ggctcaaact
     1381 gaataatccg tttgcattgg ctgcgatttt tacctttacc gaatatttac gaggcgtggt
     1441 atttaccggt tttccgtggc tacaattcgg ctatagctta atagacagcc cctttgctgg
     1501 tattgcgccg attttgggcg ttgaaggttt gactttcttg gtaatagttg caagtagcta
     1561 tctagtgaaa attattcagc aagagaacaa aactgtgcca tcattgatgg tactagcggt
     1621 catttttggc ttaagttttg ccactcgctt tctccaattt gtgcaaattg atacagaaaa
     1681 acaaccgctt gtagtgagtt tagtacaagg gaatattgag caaaaaatga agtgggatcc
     1741 gcaacatttc aaccacagcg tacgaaccta cgaacagctc attctgcccc tattaggtaa
     1801 cagtgaactg attattttgc ctgaatccgc cattccggca cttgaaaatc aaatcagccc
     1861 gctgcttggc tcgctcgatc acttagctcg ccaacacgga agtgaaatta ttatcggcac
     1921 actctatcaa aatgagcaag aggaactgtt taacagtagc gtgctattag gcgaaaaaga
     1981 gagggaatat gccctgcaca gcaaccctcg ctacaacaaa catcaccttg tgccgtttgg
     2041 cgaatatgtg ccgtttggct cggtgctgga ttggatgcgg gaagtgttta ttctaccaat
     2101 taatctttca caaggcagtt tcgttcaacc atcattcttg gcgaaaaaca accgatttaa
     2161 tatggcgatt tgctatgaaa ttatttttgg tgatcagtta caacaaaacc aaaaaagcca
     2221 aaattcagat tatttattaa ctatcaccaa tgatgcgtgg tttggttctt ccatcgggcc
     2281 ttggcaacat ttccaaatgg caagaatgcg agcgttagaa cttggcaaac cattgcttcg
     2341 agctgcgaat accggtatta ccgccattgt tgatgccaac ggaaaagtaa ttgaacaact
     2401 accgcaattt actgctgatg cactaactgc caccattgtg ccaaccaaag gcgatacgcc
     2461 gtttaaacaa ttaggtcact ggttaattta cagtttcagc ctattttgtg tcattgccag
     2521 cctgttacta cgcagaaaat aaaaaaatcc ccttaggctc ttgggtctaa ggggatttta
     2581 tcgctgatta cgctaaggct tttaataggt tcgccatttc aatcgctacc attgcacact
     2641 cttcgccttt attaccggct ttagtgcctg aacgctcaat cgcttgctct aaattttcca
     2701 ccgttaaaat accgttaata atcggcacgc cggtttctaa agcagaagag ctgatgcctt
     2761 ttgctgactc gttcactacc acatcatagt ggctggttgc tccacgcacg accgcaccta
     2821 agcagataat cgcatcataa cgaccgcttt gtgccatttt tttcgctgca attgagattt
     2881 ctaatgcacc cggcacccat acggtgtcga tatttttttc ttctacgccg tgacgcaata
     2941 atttatcggt tgcaccgttt aacagttgat cgataaaaat cttgtgccag ttagttgcca
     3001 caataccgaa ttttaaacca gttgcgatgt agtttccttg aaatgttgcc attgtttgtt
     3061 ttccttcttt aaaataaatt tgatactcaa taaccctctc cccttgtggg agagggatag
     3121 atttttcttc gttcagaaga aaaatcaggg agaggggaaa ttgcaaactt tttctaaaaa
     3181 atataccctc tctctgaaaa atacttctga acgaagtatt tttctgtctc tctcccacaa
     3241 ggggagagag tattggatta gaaattaaac atatgtcgca ttttgtgctg tttaacttta
     3301 aggtattcca aatcgtgttc gtttggctca accataatcg gctcacgagc cactacgttt
     3361 aaaccttgct caatgagtcc ttcaattttt gccggattat tggttaacaa acgaatcgat
     3421 tttacgccta atttttcaaa catttgagcg gcgatataat attcacgctc atcatctttg
     3481 aaacctaatg ccaggttggc ttcaaccgtg tccattcctc tatcttgcaa ttcgtaagca
     3541 cgcagtttat taattaaacc gatgccacgt ccttcttggc gtaaataaag caccacgcct
     3601 ctgccctctt gctccacttg gttcattgcc gctgcgaact gctgaccaca atcgcaacgt
     3661 tgtgagccaa aggcatcgcc ggttaaacat tcggaatgga tacggcataa tacattttcg
     3721 ccatcaccaa tatcaccttt aactaaagcg acgtgttctt tgcctgaaat agtttcaacg
     3781 aaactgtgtg ctttgaactc accgtatttg gtcggcattt ttaccaccga aaccacattc
     3841 accaagctgt cgtgttttct acgatactct tgtaactgct gaatagtaat aaacggcata
     3901 ttgtgtgctt gtgcaaatgc ctgtaattcc ggcatctgca tcatcgtgcc gtcttctgcc
     3961 ataatttcac agcaaagccc tgccggtttc aagcccgcca aacgagctaa atccacagtt
     4021 gcttcggtat gaccgttacg cactaatacg cctccgtctt ttgccactaa tgggaaaaca
     4081 tgccccggac gtctaaaatc ttccggctta gcattttcat caagcatttt caggcaagtt
     4141 aatgaacgct caaaagcgga aataccggtg ccggtttgaa tatggtcaac agaaacggta
     4201 aatgcggttt gatgattatc ttcgttctct agcaccatcg ggtgaaaatt taattttttg
     4261 gcaatttctg tcgaaatcgg cgtacaaatc aaccctttac catatttcgc cataaagttg
     4321 atattttccg gtgtcgcaaa ttccgctgca caaatgaagt ccccttcatt ttcacggtcg
     4381 gcatcatcgc tgactaaaat aattttccct tgtgcaattg cttgaattgc ttcttctacc
     4441 gttgaaaact taaacattat ttgtccttta tttggttaaa accctgcatt tttaagaaag
     4501 tccgcagtaa ttttactttg cggattttca ggctcttttt tcaataaaaa ctgttcgata
     4561 tatttgccga caatgtcatt ttctaaattc accaaactac cgattttttt cgtaccgagg
     4621 ttggtttcct taatggtatg cggaataatc gatacccgga aagattccgc atcaacatcc
     4681 accaccgtta aactaatgcc atcaatagta attgagcctt tttcaatgat gtaacgcatt
     4741 aacttaggtt cggctttaat gcgataccaa gttgaattat ctgctggtgt aatctctgca
     4801 accacacccg tgccatcaat atgacctgac acaatatgcc caccaaaacg cccgtttgcc
     4861 gccattgccc gctcaagatt aaccgagctg ttgatactta attcgccaag tgatgtacgt
     4921 tttaaggttt ctgacataac atcagcagta aattgctcct gcgtaaatga agtcacggtt
     4981 aaacacacgc catttaccgc aatgctatcc cctaaatgta catcggtcag aatctttttt
     5041 gctttaaccg ttaccaccac aaactcgcct tgtttcttaa tttgggctat ctggccaact
     5101 tcttcgataa tgcctgtgaa catttaaatt tttctccgat ttacttccaa tttagtctga
     5161 gaactgctta tcttactatt cccctctccc gtttacggga gagggaagag aaaatccgta
     5221 ggattttcgg agggagaggg aagattacac tcattttccc ctctctctgc ataaaatttc
     5281 tgaacgaaat tttatgctgt ctctctcccg taaacgggcg agagtaagga aatttcaatt
     5341 taattacgaa gcaagtccac ttcataatcc aacaaaatat cttctccgag caattcggtg
     5401 gattttagac gtaatttaac cgcttgtgct aactcgccaa tccccttgcc accaatcggg
     5461 gtttttgccg ctttgccgcc aatcagtttc ggggcaatat agcaatgtac tcgattgact
     5521 aacccctgtt ccagcacgct gaaattgagt tgcgaaccac cttcaatcaa taagctgtcg
     5581 atcccgagtt cgcccagctt ttgcaaaaga tcggctaaat ccacccgctt gtcacgttcg
     5641 cctgtcacta aaatttcaac accgcaagcg gtatattctg cctgcttttt tgcatcatta
     5701 ttgagcgtag caataatggt gcgatattgg cgtgcagttt ggactagttg gctatccaat
     5761 ggggtacgca gttggctgtc acatacaatc cgcaccggct gtttagcatt ttccattcgg
     5821 ctgttgagca ttgggtcatc ggcaagcacc gtttccaccc cgaccataat cgcactatat
     5881 tcgtggcggc ttttctgtac attagctcgg gcagcctcgc cggtgatcca tttcgaattc
     5941 cccgtataag tcgcaatttt accatctgcc gtcatcccat atttcagcaa cacataaggg
     6001 cgtttggttt gaatgtaatg gaagaaaatc gggttcagcg catcgcactc cgttcgcata
     6061 aagtcttcca caacctcaat gccggcggct ctaagctgtg ccgctccttt gccagaaaca
     6121 agtgggttcg gatctcgaga gccgataaac acttttttga tcccacgttc aatcagcagt
     6181 tcggaacaag gcggggttcg cccgtggtgg caacaaggct ctaaggttac ataagcggtt
     6241 gcccccgtca gatcttcctc gcagtttaaa atcgcattgc gttcagcgtg ccaactaccg
     6301 gctttttggt gatacccttc ggcgataatg ttgccgtttt tcacgatcac acagccaact
     6361 agcggattag ggcttgtcca accccgtgct ttttcggcaa gggtgatggc ataagccata
     6421 tattgaacat ctgtcatata cattttgata aattgagggc gtaatgaaag gattattgca
     6481 atacaagcgg tcgtttttag taaaaaattt gcaaacgccg cctgcaaaac aatgaagcct
     6541 tgaaatctca cgactcaagg cttgcaaaaa agacacaaaa aatgaccgct tgtgcggtat
     6601 tttttgcggt ttttgactct tctttcatcc agactatact gtcggttttg gaatttcacc
     6661 aaatcctgcc gattgctcgg ctcgcggact ttaccgccga tcgggaattt caccctgccc
     6721 tgaagattaa aatatgcagg ggattttgct cgcattacga tttgatgtca agcgggaaat
     6781 taagctaatt accctgattt taacaattga ttaaatcgcc aacatctact gttttgacta
     6841 ttcgagtttt tttaagataa atcaaatttt cccttgtttt tcccaaattc gccctcataa
     6901 tgggggatta tattttgata gagagagcaa atttatggca ccaagaaaaa agaaagcgat
     6961 tgaattacca ctactcccat tgcgtgatgt ggtggttttt ccttatatgg ttatgccgtt
     7021 atttgtagga cgtgaaaaat caattcaagc cctacgctcg gcgatggatt ccaataaaca
     7081 actgttttta gtcactcagc aagatccaaa taaagaagag ccgaatgcag aagatatgta
     7141 tggcgtaggt gtaattgcca acattattca aatgttaaac ctgccggacg gcacagttaa
     7201 agtgctggta gaaggacaaa cccgtgccaa aatcgagcaa attcacgatg atgaaaacgg
     7261 cttctgggcg gcaattcagc caatatattc ggaatatgat gacgaaaacg aagagttaaa
     7321 agcgattgct aaaaccactt taaccgaatt tgaaaattac gttaaaaata acaagaaaat
     7381 tccggcggaa attattgcta aactgcaaaa aatcacgttg gaagaccgct tggcggatac
     7441 cattgcctct aacttaattg ctccggtgaa gaaaaaacag gaattattgg agcaaccaaa
     7501 tctgattgcc cgttttgaag ccttgttaat tgcgatggca actgagatgg atacgcttga
     7561 aaccgaaact cggattcgta atcgtgttaa acagcaaatg gaaaaaaacc aacgtgatta
     7621 ctacctcaac gagcaaatta aagcgattca aaaagagtta ggtaatggcg atgacgttga
     7681 acaaagcgag ttagataagc ttaaagagaa aattgacgct gcaaaattac cgaccgaggt
     7741 aaaagacaag ctgaatacgg agttcaaaaa gctcaaagca atgccgcaaa gctcttccga
     7801 agctaccgtt gtgcgtggtt atattgactg gattttacaa atgccgtggc ataaaaaatc
     7861 agcggtcaaa aaagatttag cccaagctca agagattttg gataaagacc actacggctt
     7921 agaacgagtg aaagagcgga ttgtagaata cttagcagtg caaagtcgtc taaataaatt
     7981 aaaaggtccg attttgtgct tagttggtcc gccgggggtt ggtaaaacct cattagggca
     8041 atctattgcc aatgccacag ggcgtaaata tgtgcgtatg gcgttaggtg gcgtgcgtga
     8101 tgaagcggaa atccgtgggc atcgccgtac ttacatcggc tcaatgccgg gtagcctaat
     8161 gatgaaaatg gcgaaagtcg gcgtgcgtaa tccgctgttc ttgttagatg aaatcgacaa
     8221 aatggctcaa gatatgcgtg gcgacccggc ttctgctctg cttgaagtgt tagatcctga
     8281 gcaaaataaa gcctttaacg accattattt agaggtggat tacgatttat ccgatgtaat
     8341 gtttgtggca acctcaaact caatgaacat tccgccagca ttgctcgacc gtatggaagt
     8401 gattcgtctt tcaggctata ccgaagatga gaaaatgcac attgctcgtg gtcatttact
     8461 ggctaaacag caagaaaata acggactaaa agagggtgag ctgaccattg aagacagtgc
     8521 aattttaagt attatccgct actacacccg tgaagcaggc gtgcgtagcc ttgagcgtga
     8581 aattgcgaaa atctgccgta aagccgtgaa agctttagta ttggataaga aattaaaatc
     8641 cattacagtt tctgagaaaa atatcgaaga atacttaggc gtaaaacgtt ttgactacgg
     8701 caaaatggat agccaaaacc gcattggcga agtgaccggt ttagcgtgga cagaagttgg
     8761 cggtgattta ttaaccattg aaaccgcttc tgttgcaggt aaaggcaaat tctcttacac
     8821 cggttcatta ggcgatgtaa tgaaagagtc tattcaagcg gcaatggtgg ttgtacgctc
     8881 tcgtgcagaa aaattaggca ttgctgaaga cttccacgaa aaacgggata ttcacgttca
     8941 cgtgccggac ggtgcaacac cgaaagatgg tccgagtgca gggattgcaa tgtgtactgc
     9001 attaatttcc agcctaacag gtaaccctgt gcgtaaagaa gtagcgatga ccggtgaaat
     9061 cagcttgcgt ggcaaagtat tgccaatcgg cggcttaaaa gagaaattac tggcagccca
     9121 tcgtggtggt attacgacgg tgattattcc gaaagaaaat gaaaaagact tagaggaaat
     9181 tccggaaaat gcaaaagcag cgttggacat tcacgcagta gaaaccattg acgaggtact
     9241 agctctggca ctagaaaacc cgccaatggg gattgaaatc ttgtcaaaag ccgagatcaa
     9301 ggtgaaaaaa tcgcgagcta aagccacagt gcaataaagt gcaataatct aaaacaacat
     9361 aaggtgcgca gatcacaatc acgcacctta ttttttatct gaaagtttta tctacaagcg
     9421 gtcggttttg ctctattttt tgcaaaaatt tagcaaaatc taaccgcttg ttatgctgaa
     9481 ttatcagaca ataattgcca aaatgcgttg atgatcggct cttgcaagcg tttttgctga
     9541 acgcaaattc ccagctcaaa cggagtgatc ggcgagggta aattcacata agaaacttga
     9601 ttattcattg ggctatgctt aatcacaata tccggtagca gtgcgacccc aaagcctagt
     9661 gcaaccatcg gcacaatcgc ttcgtgtccg gagacggtag cataaataat cgggtctttg
     9721 attttttgct ctttaaacca acggtcaatc cgttttcgta caggtccgtt caccggcaaa
     9781 ataaagggga tatttttcca atctatcggc gactgttgca gaaattgagt ggcttggctc
     9841 gccacacgag gggcaataat cgaaagttga atatcatcaa tatagtgaaa cacaatatta
     9901 ttcggcagaa attccggctt acccgcaacc gaaatatctg cagccaatga ctgaacctgt
     9961 tccaatgcca aagccggatc accggtactc agtttaatat ccactttcgg atatttttgg
    10021 cgaaactttc ccaaaatcgg cggtaaatgg ctgtaagaag ccgttaccga gcagaacact
    10081 ttcaactctc cttctaaatc accttgagca ggcgacaact ggttttgcaa ctgctgccac
    10141 tctgcccaac cttgttgagc aaacgccaaa aacctttccc ctgcttcagt taaacgcact
    10201 tgtcgattat cacgcagaaa caacggctgc ccgagctcct cctctatacg ctgaatatgg
    10261 cgggtaagtg tggaagccgt catataattc ttctccgcac tgcgaataaa actacggctt
    10321 tgggctatat ctaggaaaag tttaaggctt tggaagtcca tattatttta gccactccgg
    10381 ttttattgca gcaaaatcta aatcattcac gctttttcta aaacggagaa tatgggtttc
    10441 agcctgatta ttcggatgcc aatgtataaa attctgttca tcgctactgt aaaaagcaat
    10501 tatcttttta ctcaagcctg aggcaatatg cacggttgca gtatcaggtg aaattaataa
    10561 gtccgcaaaa cgaatcagtt ctattgaatc aaaaacgctt ttggttttct catagacaaa
    10621 tatattttta tacttcttag taaaggaaca caattttgcc gttacttcag gaaaagtaag
    10681 taagactatc ggtttatcac tatgctgttt taagcaactc agcagttcat taattttctg
    10741 ctcactaaaa cgtcttgccg aacctgcacc aaaaagattt aacgcaatat aatttctaat
    10801 ttggttttca tataaataat gctttatgga ttggttgctt gtggcattta gtggcacatc
    10861 atactgagtt tctatatttt caaacccgat taaagctaac gcatcttgat aaatctctga
    10921 aaaatgctgt tttctatcct ctacatttag atcaaataat cgatattttt gtttctgata
    10981 acctacataa tgtttggctg atatagtgcg taaaaataag agatcacgat tccgtaaagt
    11041 aacagtcgga tcgattacaa catcatactg ctcttttgca agttgtttgc caccacgaat
    11101 ataatcagga atactttttg tcttaaccaa atagagctta tcaatataag gattattctc
    11161 aaataaatat cggtttttct gtgagcaaac cacgcctatt tgcgtttggg gagactgttt
    11221 ttttatttca cggaagacaa aggaactaac gacataatca ccaattttac catcgtgacg
    11281 aagaaataga atttta
//
LOCUS       DANLYI010000064        11023 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.064, whole genome shotgun sequence.
ACCESSION   DANLYI010000064 DANLYI010000000
VERSION     DANLYI010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 11023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11023
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.064"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            99..1991
                     /gene="mnmG"
                     /locus_tag="PXD81_001239"
     CDS             99..1991
                     /gene="mnmG"
                     /locus_tag="PXD81_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HDL5537265.1"
                     /translation="MIYNQIYDVIVVGGGHAGTEAALAPARMGLKTLLLTHNVDTLGQ
                     MSCNPAIGGIGKGHLVKEIDAMGGLMAKATDVAGIQFRTLNSSKGPAVRATRAQADRV
                     LYRNAVRTALENQPNLDIFQQEVVDILVENNRAVGAVTKMGLTFKARAVVLTAGTFLA
                     GKIHIGLDNYAGGRAGDPAATMLADRLRDLNLRVDRLKTGTPPRLDARTINFDVLAKQ
                     HGDEVLPVFSFMGDVSQHPRQIPCYITHTNDQTHDLIRNSLDRSPMYTGIIEGVGPRY
                     CPSIEDKVMRFADRNSHQIYLEPEGLNTIEVYPNGISTSLPFDVQMGIVNSMKGLENT
                     RIIKPGYAIEYDYFDPRDLKPTLETKAIDGLFFAGQINGTTGYEEAGAQGLLAGINAA
                     LQVQGKEAWYPTRDQAYTGVLVDDLCTLGTKEPYRVFTSRAEYRLLLREDNADIRLTP
                     KAHELGLIDEARWVRFNQKMEAIEQERARLKSTWAHLQMPNLDELNALLANPLAREAS
                     GEDLIRRPEMSYEKLTQIALFAPAIEDKQAAEQVEIAIKYQGYIEHQYNEIERHKRHE
                     NTLIPVEFDYDKVESLSNEVRAKLMQHRPVSIGQASRISGITPAAISILLVNLKKQGM
                     LKRGEL"
     gene            2086..2310
                     /locus_tag="PXD81_001240"
     CDS             2086..2310
                     /locus_tag="PXD81_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5537266.1"
                     /translation="MIYLRKLGFSPFDHQMFAQPSWYFCNALVHTNYENLRQGVFVTK
                     AYLVMFLENLLFEKSHSLSNRALRINENND"
     gene            2323..2937
                     /gene="rsmG"
                     /locus_tag="PXD81_001241"
     CDS             2323..2937
                     /gene="rsmG"
                     /locus_tag="PXD81_001241"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005602408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HDL5537267.1"
                     /translation="MLAKLNRLLAQAEINLTDQQKEQLVGFVRLLDKWNKAYNLTSVR
                     NPDEMLVKHILDSLVVSRHLQGETFIDVGTGPGLPGIPLAIANPDKQFVLLDSLGKRI
                     TFIKNALRELGIKNVTPVQSRVEEYTESQFDGVLSRAFASLNDMIDWCYHLPTENGKF
                     YALKGVYDEQEIIEIKQPIQLVEVIKLEVPELVGERHLVVLKKP"
     gene            3205..4127
                     /locus_tag="PXD81_001242"
                     /pseudo
     CDS             3205..4127
                     /locus_tag="PXD81_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248294.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            4150..5196
                     /locus_tag="PXD81_001243"
     CDS             4150..5196
                     /locus_tag="PXD81_001243"
                     /inference="COORDINATES: protein motif:HMM:NF016497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HDL5537268.1"
                     /translation="MQIQNPVLPGFNPDPSIVRVGDTYYIATSTFEWFPGVRIHESKD
                     LVHWNLVKNVLDTTTLLDMRGNPSSGGIWAPDLSYAEGKFWLVYTDVKITDGAFKDMT
                     NYLTTAKEIQGPWSAPIKLNGIGFDASLFHDDDGRKYLVQQTWDHREYHHPFDGITLT
                     EFDVATMKLKPETQRTIFEGTEVKLVEGPHIYKINGEYYLFAAEGGTVFTHQEVVARS
                     KTLEAHSFQLAPNTPFITNFDTPNSYLQKQGHGSLVDTPSGEWYYASLTARPWNHATE
                     SATDPRGWSTLGREAAIQKVEFDQDGWPYIVGGHGGKTFVEAPKDAIFTEAPIDHSQH
                     DEFDSSTLDINWNT"
     gene            5199..5837
                     /locus_tag="PXD81_001244"
     CDS             5199..5837
                     /locus_tag="PXD81_001244"
                     /inference="COORDINATES: protein motif:HMM:NF036826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-1,4-xylosidase"
                     /protein_id="HDL5537269.1"
                     /translation="MPFSKEMGEVGNGVLKLIGRGSLANTHDLSLIARRWQAFYFDAE
                     TKVKFTPFSYQSMAGLTNYYNHSHWSWIFITKNDQGQQVIEVAENKGGLRNGQYTSYL
                     KDKAIVIPDGTEYVWFKTKVRKETYRYEYSFDGKQWHTIPVELDAAVLSDDYVLRSYG
                     GFFTGAFVGLAAVDYSSYESTAEFSYFDYKELGDKQLTDGSFSWEKSESCFG"
     gene            5856..6836
                     /locus_tag="PXD81_001245"
     CDS             5856..6836
                     /locus_tag="PXD81_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDL5537270.1"
                     /translation="MKQRRFGKTNKMVSEIGLGTWQLGTKWGEPFNHQEAMAILETAY
                     EQGINFIDTADVYNNGQSEKAIGDILKKYPDFFYVVTKCGRALNPHTAEMYTPQAIEK
                     FVDGSLSHLGTEKLDMILLHCPPTLVYRNDEIFTKLEQLKAGGKLIDYGVSIETAEEG
                     LLAMEYDIAAMEVIFNMFRLKPLEQLFPTAQQKNVGIIARVLLASGLLTGRYNACTQF
                     GKDDHRTFNRNGEAFDKGETFSGVDYQLGLQAVEELKALFGTEDLIPYALRWVLMNEA
                     VSTVIPGASKVSQVITNAAVDNFPPLTENEMQKVEDIYNRLIRPSVHHLW"
     gene            complement(6898..8238)
                     /locus_tag="PXD81_001246"
     CDS             complement(6898..8238)
                     /locus_tag="PXD81_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL5537271.1"
                     /translation="MFSFLKASPPAEQKVEASRVDAEYRKLRWQVFAGVFIGYAAYYL
                     IRKNFSLAMPYLIDEYGFTKADLGTVGVALSLAYGFSKFIMGNVSDRSNPKYFITIGL
                     LGSAIVSLVFGLVPGVLSSIPIMIILAALNGWFQGMGYPPGAKTMTNWFSTSERGVWW
                     SWWNVSHNLGGGLIGPLAILGLSIFGVWQSLFYLPALIAIVLAFVMFYLMRDTPESQG
                     LPPIDEYKGEKVVEKVESAHTLSSKDIFYKYILNNKFLWAIAIANVFVYFIRYGIIDW
                     APTYLKEVKHFSVDKQSWAYFLYEYAGIFGMLASGYLSDKVFKGHRAPPMLLFLAGVL
                     IAIVVYWKNPAGNPLIDNICLVAIGFLIYGPVMMIGLQAADLVPRVATGTATGLTGLF
                     GYLLGSASAGYVMGKLVDMFGWDGGFYALILSCFLAFAFIALTLFKKTSKQLEV"
     gene            complement(8428..9501)
                     /gene="glpQ"
                     /locus_tag="PXD81_001247"
     CDS             complement(8428..9501)
                     /gene="glpQ"
                     /locus_tag="PXD81_001247"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HDL5537272.1"
                     /translation="MKFKFKTLVAALALSATALGAYAQQSDKLVIAHRGASGYLPEHT
                     LESKALAFGQQADYLEQDLAMTKDNRLIVIHDHFLDGLTDVAKKFPERKRADGRYYVV
                     DFTLDEIKTLEMTENFEVKDGKQAQVYPNRFPMWQSHFTIHTLEEELEFIQGLEKSTG
                     KKIGIYPEIKAPWLHHKEGKDIALETLKVLKKYGYDSKTSPIYLQTFDFNELKRIKTE
                     LLPKLGMDVKLVQLVAYTDWHETEEKDKNGNWVNYDYDWMFKDGAMAEVAKYADGVGP
                     GWYMLVDKEKSKVGNIVYTPLVKELAKYKMELHPYTVRKDALPDFFQNVDEMYDALLN
                     KAGATGVFTDFPDTGVEFLKKQK"
     gene            complement(9571..11016)
                     /gene="glpT"
                     /locus_tag="PXD81_001248"
     CDS             complement(9571..11016)
                     /gene="glpT"
                     /locus_tag="PXD81_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HDL5537273.1"
                     /translation="MFGPFKPAPHIAELPADKVDARYKFLRWQVFAGIFFGYAAYYFV
                     RANFDLAQKGLIEAGMYTKTDLGVIGTAAGLAYGLSKFVMAGMSDRSNPKVFLPTGLL
                     LSGLCMTLMGLMPWATSSILVMWVMIFLNGWFQGMGWPPCGRTMVHWWSKSERGTIVS
                     IWNTAHNLGGMVPGAMVLLASALYFSEYGVEATAKDVWQQALYFPGIAAMIAAIPIYL
                     VMKDTPQSCGLPPVEKWRNDYPDDYNEKTYEHDLSTKEIFVTYVLKNKLLWYIAIANV
                     FVYLIRYGVLKWSPVYLGEVKHFNIKGTAWAYTIYELAAIPGTLLCGWVSDKLFKGKR
                     GLTGFIFMILTTAAVVAFWLNPATPESEIAQYAGHAWYENPYQLMDFIWMTTIGFLIY
                     GPVMLIGLHALELAPKKAAGTAAGFTGLFGYLGGTVAASAVVGWAADKFGWDGGFYVM
                     IGGGVLAILLTFIVMVEEGKHKAKLEDHYGK"
BASE COUNT         3185 a         2334 c         2384 g         3120 t
ORIGIN      
        1 cctagcgttg ctaaaaatct atttccctga tgatcccttt ttacgctaaa atatgcgttt
       61 actttgtagc agaatttatt tgaataaaaa ggcgatatat gatttataac caaatttatg
      121 acgtgatcgt tgttggtggc ggtcacgccg gaactgaagc agcacttgct cccgctcgaa
      181 tgggcttaaa aaccctgctt ttaacccata atgtagatac cttaggacaa atgtcttgta
      241 acccagccat cggtgggatc ggtaaaggtc atctggttaa agagatcgat gcaatgggcg
      301 gtttaatggc aaaagccact gatgttgccg ggatccaatt ccgtactcta aacagctcga
      361 aagggcctgc agtgcgtgct actcgagcac aggcagaccg tgtgctttac cgcaatgcag
      421 tgcgtacagc attagaaaat caaccgaatt tagatatttt ccagcaagaa gtggtcgata
      481 ttttggtgga aaacaaccgt gcagtgggtg cagttaccaa aatgggctta acgtttaaag
      541 cccgtgcggt agttttaacc gctggtacgt tcttagcggg taagatccac attgggttgg
      601 ataactatgc aggtggtcgt gccggcgatc ctgctgcaac aatgttggca gatcgtctgc
      661 gtgatctgaa tttgcgtgtt gatcgcctta aaacaggtac accacctcgc ctcgatgccc
      721 gtaccatcaa ctttgacgta cttgccaaac agcacggcga tgaggtgtta cctgtcttct
      781 cttttatggg cgatgtctca cagcacccac gccaaattcc gtgctacatc acccacacta
      841 acgatcaaac ccacgattta attcgcaaca gtcttgatcg cagcccgatg tacaccggta
      901 ttatcgaagg ggttggtcca cgttactgcc cgtctattga agataaagta atgcgttttg
      961 ccgatcgtaa tagccaccag atctaccttg aaccggaagg cttaaatacg attgaggttt
     1021 acccgaacgg gatctccacc agcttgccgt ttgatgtgca aatggggatc gtcaactcaa
     1081 tgaaaggctt ggaaaacacc cgcatcatca aaccgggcta tgcgattgag tacgactact
     1141 tcgacccgcg tgatttaaaa ccaacccttg aaaccaaagc gattgacggt ttattctttg
     1201 ccggacaaat taacggcacg accggttacg aagaggcagg tgcgcaaggt ttgctggcag
     1261 ggattaacgc agcgttacag gttcaaggta aagaggcgtg gtatccaacc cgtgatcaag
     1321 cctacaccgg cgtgttggtg gacgacctct gtacattagg caccaaagag ccataccgtg
     1381 tgtttacctc tcgtgccgaa taccgtctgt tgctgcgtga agacaatgcg gacattcgtt
     1441 taacaccaaa agcccacgaa ctaggattaa ttgacgaggc acgttgggtg cgtttcaacc
     1501 aaaaaatgga agcgattgag caagaaagag ctcgtttgaa atccacttgg gcacatttgc
     1561 aaatgccaaa tctggacgaa ttgaacgcgt tattggctaa tcctttagcc cgtgaagcaa
     1621 gcggtgaaga cttaatccgc cgtcctgaaa tgagttacga aaagctgacc caaatcgccc
     1681 tatttgcccc tgctattgaa gacaagcaag cggcagaaca ggtggaaatt gcgatcaaat
     1741 atcagggcta tattgagcat caatacaacg aaatcgaacg ccataaacgc cacgaaaaca
     1801 ccctgattcc ggtagagttt gactacgaca aagtcgaaag cctctccaac gaagtgcgag
     1861 ctaaattgat gcaacaccgc cctgtctcca tcgggcaggc aagccgcatt tccggcatca
     1921 caccggcagc gatttccatt ctgttggtga atttgaagaa gcaaggaatg ttgaaacggg
     1981 gtgagctgta aatactacaa gcggttggtt ttgcagaaat ttttgcaaaa ttgaccgctt
     2041 gtattctttg gagaaggaaa tacccgaact accgccgtat ttttaatgat atatctgcgt
     2101 aaactcggtt tttcgccgtt cgatcaccaa atgtttgccc aaccttcgtg gtatttctgc
     2161 aatgcacttg ttcataccaa ttatgaaaat ctgcgacaag gtgtatttgt gactaaagcg
     2221 tatttagtga tgtttcttga aaatttattg tttgaaaaaa gtcattcgtt aagtaatcgg
     2281 gcattgcgta ttaatgaaaa taatgattaa ggaatttgca aaatgttagc taaattaaac
     2341 cgcttgcttg ctcaagccga aataaatttg accgatcaac aaaaagagca gttagttggt
     2401 tttgtgagat tattggataa atggaataag gcgtataacc tcacttccgt tcgcaaccct
     2461 gatgaaatgt tggttaaaca tattttggac agccttgttg ttagtcgtca tttacaaggc
     2521 gaaacgttta ttgatgtcgg cacggggccg gggctaccgg gcattccgct ggcgattgct
     2581 aaccctgata aacagttcgt gctactagac agcttaggca aacgcatcac ttttatcaaa
     2641 aatgcgttgc gtgagcttgg tattaaaaac gttaccccag tgcaaagtcg agtggaggaa
     2701 tacactgaaa gccagtttga cggcgtatta agccgagcct ttgcctcgct taatgatatg
     2761 atcgactggt gttatcattt accgactgaa aacggtaaat tttatgcatt aaaaggcgtg
     2821 tatgatgagc aggaaataat tgaaatcaaa caaccgattc agctagtgga agtgattaag
     2881 cttgaagtac ctgagttggt aggagaacgg catttagtag tgttgaaaaa gccctaatac
     2941 attggaagtt taatgataca agcggtcgaa tttgtaaatt tttttgcaaa tctgcctgct
     3001 tgtgttaact atttttcata aagcatacct ggttaataag gtgtgctttt tttataacct
     3061 attatttcat ctgatttatt tctattttgt gatgtgcttc acaaaaacaa aaaaacataa
     3121 tgtagtaaaa aatattcgta attgaagccc ttatttcttt acctgaaaat aagcgaactt
     3181 aattatataa cgaggaggtt cgtaatgaac agggtaacaa ataaggcaga tgagtttgct
     3241 aaactcagta cgtttgaacg tattgcttat ggtagtggcg acttagccca aaatttagtt
     3301 tttggtacag ttggtggttt cttactcact tacttaacta ctgtaaacgg catttctgcc
     3361 gtagcaggag caacgatttt cttaattgta aaatggggaa atgtgttttg ggatccgttt
     3421 gtcggtgcct ttattgacaa aagcaagccc tcagcacagg gtaaataccg cccgtattta
     3481 ctgaaatttg ggattcctct tgtggtttta gcttcactac tatttcttcc tcttacctaa
     3541 aatataatat tgggcattct gagtggattg cctccgtcgg tttattaggc tctatccctg
     3601 ggattttcct tgtattctta tggccgaaac tacgtcgcac tatcggtaaa aagaactttt
     3661 tctatctatt tttagggatt ttcgttctag ggcaactttg tctatgggta tggcaatttg
     3721 actccttcaa atactctcca accttaggct atatcggtcg tttcttacaa caatggggat
     3781 taacttctgc taccggtttt atgtgggcgt tagtgcctga ggttgttacc tttggagagt
     3841 ttaagtcgaa aaaacgtgta gcaggtatta ttaatgcgtt aatgggcttg ttctttaaaa
     3901 tcggcttggc gttaggcggt attattccgg gttatattaa tgcggtttat ggttttaacg
     3961 gctcgctttt ggctcaaacc gaagaggctt tatttggtat caatctttca atgatttggg
     4021 ctccaattat tttagcgatt attgcaggat ttgtgatgag tcgctatccg ttaagcgata
     4081 ctgatattga tcatatcaac gaaaaaatta ctgcttattc caaataattt gagtatagga
     4141 gaatagatta tgcaaattca aaatccagtt ttacccggtt tcaatccaga tccatctatt
     4201 gtgcgtgtgg gtgatactta ctacattgca acttctactt ttgaatggtt tccgggagtg
     4261 agaattcacg aatcgaaaga tttagtgcat tggaatttag ttaaaaatgt cttagataca
     4321 accacgttgt tagatatgcg aggcaatccc tcttcaggtg gtatctgggc tcctgatctt
     4381 tcttatgcgg aaggtaaatt ttggttggtt tataccgatg taaaaattac agacggtgca
     4441 ttcaaagata tgactaacta tctaactact gccaaagaaa ttcaaggtcc ttggtcagct
     4501 ccgattaaat tgaatggtat tgggtttgat gcttcgttgt ttcacgatga tgatgggcga
     4561 aaatatttag tgcaacagac ttgggatcac cgtgaatatc atcacccctt tgacggtatt
     4621 acgttaacgg aatttgatgt tgccacaatg aaactcaaac ctgaaaccca gcgtacgatt
     4681 tttgaaggta cagaagtgaa attggtggaa ggtccacata tttataaaat taatggcgaa
     4741 tattatctgt ttgcggcgga aggcggcaca gtatttacgc atcaagaagt agtggctcgt
     4801 tctaaaacat tggaggctca ctctttccaa ctggctccaa atacaccgtt tattactaat
     4861 tttgatacac ctaattctta cctacaaaaa caagggcacg gttcattagt tgatacccca
     4921 agtggtgaat ggtattacgc ttccctcaca gcaagacctt ggaatcatgc aacagaatcc
     4981 gcaaccgatc ctcgtggttg gtcaacatta gggcgtgaag ccgccattca aaaagtagaa
     5041 tttgatcaag acggctggcc ttatattgtg ggtggacacg gtggaaaaac ctttgtagaa
     5101 gccccaaaag atgcaatttt taccgaagct cctattgatc attctcaaca cgatgagttt
     5161 gatagctcaa cgttagatat taactggaac acttaagagt gccttttagt aaagagatgg
     5221 gcgaagttgg taatggtgtt ttaaaattga ttgggcgagg ctctcttgca aatacacacg
     5281 atttatcgct aatcgcacgc cgttggcaag ccttctattt tgatgcggaa accaaagtga
     5341 aatttacacc gttttcttat caatctatgg caggtttaac taactattac aatcactcac
     5401 actggtcttg gattttcatc accaaaaatg atcaaggaca acaggtgatt gaagttgcag
     5461 aaaataaagg tggactcaga aatgggcaat atacttctta ccttaaagat aaagcaattg
     5521 tcattcctga tggaacggag tatgtttggt ttaagactaa agttcggaaa gaaacttacc
     5581 gttatgaata ttcattcgat ggcaaacaat ggcacactat tccggttgaa ttagatgcgg
     5641 cagtgctttc agatgattat gtgttacgca gttatggcgg tttcttcaca ggagcttttg
     5701 tcgggctggc tgcagtggat tactccagtt atgaatctac agcagaattt agctactttg
     5761 actataaaga gttaggcgat aagcaactta cagacggctc attctcttgg gaaaaatctg
     5821 aaagttgctt tggctaaaat gaggagaata tcacaatgaa acaacgcaga tttggtaaaa
     5881 ccaacaaaat ggtgtcagaa attgggttag gcacttggca gcttgggacg aaatggggtg
     5941 aaccgttcaa tcatcaagag gcgatggcaa ttttagaaac cgcttatgag caaggcatca
     6001 attttattga taccgctgat gtgtataaca acgggcaaag cgagaaagcc attggtgata
     6061 ttttgaaaaa ataccctgac tttttctatg tcgttaccaa atgtggacgt gcgttaaacc
     6121 cgcatactgc tgaaatgtac acgccacagg caatcgaaaa atttgttgat ggtagtttaa
     6181 gccatcttgg cacggaaaag ttggatatga ttttgctcca ctgcccgcca acgttggttt
     6241 atcgcaatga tgagattttt acgaaattgg aacagcttaa agccggcgga aaattaatag
     6301 actacggtgt gagtatcgaa acggcagagg aaggcttgtt ggcaatggag tatgacattg
     6361 cggcaatgga agttatcttc aatatgttcc gcttaaaacc attggagcaa ctcttcccaa
     6421 ccgctcagca aaaaaatgtg ggtattatcg cccgtgtact gttggcaagt ggtttgctta
     6481 caggcagata taatgcctgc acccaatttg gcaaagacga ccaccgcaca tttaaccgca
     6541 atggtgaagc cttcgataaa ggcgaaacct tttcgggtgt ggattatcaa ctcggtttac
     6601 aagcggtgga agaattgaaa gccctattcg gcacggaaga tttaattcct tacgccctac
     6661 gttgggtatt gatgaatgaa gctgtgagta cagtaatccc gggagcaagt aaggtttcac
     6721 aagtaattac taacgcagcg gtggataatt tccctccact cactgaaaat gaaatgcaaa
     6781 aagtggaaga tatttacaac cgcttaattc gcccaagtgt gcatcatttg tggtaattta
     6841 tccaacgcta taaaaaatgg aactctaact tgagttccat ttttgtcaat tttagcctta
     6901 aacctctaac tgtttagagg ttttcttaaa cagagtcaat gcaataaacg caaaggctaa
     6961 gaaacaggaa aggatcaggg catagaaacc accgtcccag ccgaacatat caaccagttt
     7021 acccatcaca taacctgcac ttgcagagcc taacaagtag ccgaataaac cggttaaacc
     7081 tgttgctgta ccggtggcaa cacgaggcac taaatccgct gcttgtaaac cgatcatcat
     7141 caccggcccg tagattaaga aaccaatcgc cactaagcag atgttgtcga ttaacgggtt
     7201 gccggctggg tttttccagt acaccacaat cgcaattaac acaccagcaa ggaataacaa
     7261 catcggcggc gcacggtgac ctttgaatac tttgtcgctt aaataaccgc ttgcaagcat
     7321 accgaagata ccggcatatt catacaagaa gtacgcccag ctctgtttat ccacagagaa
     7381 atgtttcact tcttttaagt aagtcggtgc ccagtcgata atgccgtagc gaatgaaata
     7441 gacaaacacg ttggcaatcg caattgccca caggaatttg ttgtttaaaa tatatttgta
     7501 gaaaatgtct ttggaagaga gtgtgtgggc agactccact ttttccacca ctttctcgcc
     7561 tttgtactca tcaatcggcg gtaagccttg agattccggc gtatcacgca tcaaatagaa
     7621 catcacaaac gccagtacaa tcgcaatcaa cgccggcaga tagaacaggg attgccacac
     7681 gccaaaaata gataagccta aaatcgccaa cggcccgatt aaaccgccgc ccaagttgtg
     7741 cgacacgttc caccagctcc accacacgcc acgttcagag gttgagaacc agttggtcat
     7801 cgttttcgcc cccggcggat agcccattcc ttggaaccag ccgtttaacg ccgccaaaat
     7861 gatcataatt ggaatagacg aaagcacgcc cggtactaaa ccgaacacca gactcacaat
     7921 cgctgaaccg agcaagccga tagtgataaa gtatttcggg ttagaacggt cggaaacgtt
     7981 acccataata aatttactga agccgtaagc gagtgataat gccacgccca ccgtacctaa
     8041 gtctgctttg gtgaagccat attcatcaat caaatacggc attgccaacg agaagttttt
     8101 acgaattaaa taatatgccg catagccgat aaagacgcct gcaaacactt gccaacgtaa
     8161 tttgcgatat tccgcatcaa ctctgctggc ttccactttt tgttctgctg gtggagaggc
     8221 ttttagaaag gaaaacattt tactactcct ttaagaatga aatcctatgg ctactataat
     8281 ctttcatttg gagcagaaaa agtggatttt tgaaaatttg tgagcaagat taaaattatt
     8341 ttgagcgaaa acgaaaagcg gttgaatttc cttctttttt tgcaaaaaat ttgagaaatc
     8401 taaccgcttg tttcacttaa aaatcagcta tttctgcttt ttcaaaaatt ccacgcccgt
     8461 atcagggaag tcagtaaaga cgcctgttgc acccgcttta ttcagcaggg catcgtacat
     8521 ttcatcgaca ttttggaaaa agtccggcag agcatctttg cgcacggtgt atgggtgaag
     8581 ttccattttg tacttcgcca actctttcac taatggcgtg taaacaatgt tgccgacttt
     8641 agatttttct ttatccacca acatatacca gcccgggccg acaccatcgg catattttgc
     8701 cacttctgcc atcgcaccgt ctttgaacat ccagtcgtag tcgtaattaa cccagttgcc
     8761 gtttttatct ttctcttcgg tttcgtgcca atcggtgtag gcaactaact gcaccaattt
     8821 tacgtccata cccagtttcg gtagtaattc ggttttgatc cgtttcagct cgttgaaatc
     8881 gaaggtttgc aggtaaattg gtgaggtttt gctgtcgtag ccatattttt tcaacacttt
     8941 cagggtttcg agtgcaatgt ctttaccctc tttatggtgc aaccaagggg ctttaatttc
     9001 aggatagatc ccaatttttt tgccggttga tttctctaat ccttgaataa attccagctc
     9061 ttcttccaaa gtgtgaatgg tgaaatggga ctgccacatc gggaagcggt tcgggtaaac
     9121 ttgtgcctgc ttgccgtctt tcacctcaaa attctcggtc atttcaaggg ttttaatctc
     9181 atcaagggtg aaatccacca cataataacg accgtctgct cgtttacgct cagggaattt
     9241 tttcgccaca tcagttaaac cgtctaaaaa gtggtcgtga atcacaatca ggcggttatc
     9301 tttggtcata gcaagatctt gctctaaata gtccgcttgt tgcccgaatg ctagagcttt
     9361 agattcaagc gtatgctcag gtaaataacc gcttgcacca cggtgggcaa tcactaattt
     9421 atcagattgt tgtgcatagg cacccaatgc tgtcgcggac aatgctaatg cagcaaccaa
     9481 tgttttgaat ttaaatttca tagaattacc tcatataatg taaaaataaa aggaagaaca
     9541 aaaatgtcct tccttttgat aagttattta ttatttacca tagtgatctt ctaattttgc
     9601 tttgtgcttg ccttcttcaa ccatcacaat gaaggttaag agaatggcta atacaccacc
     9661 accgatcatc acgtagaaac cgccgtccca gccaaattta tctgctgccc agccaactac
     9721 tgcagaggct gccactgtac cacctaagta accgaataaa ccggtgaaac ctgctgctgt
     9781 acctgctgct tttttcggtg caagttcaag ggcgtgtaag ccaatcaaca ttaccgggcc
     9841 gtagattaag aaaccgatag tggtcatcca aatgaagtcc attaattgat atgggttttc
     9901 ataccaagca tggcctgcat attgtgcaat ttcagattcc ggtgtcgcag ggttgagcca
     9961 gaacgcaacc actgctgcgg tggtaagaat cataaagatg aaaccggtta aaccacgttt
    10021 gcctttgaac agtttgtcag agacccaacc acagagtaat gtacctggaa tcgcagctaa
    10081 ttcataaatg gtatatgccc atgctgtacc tttaatgttg aagtgtttta cttcgcctaa
    10141 gtacaccggc gaccatttca atacgccata gcggattaag taaacgaaga cattcgcaat
    10201 ggcaatgtac cataacagtt tgtttttcag cacataagtc acgaaaatct ctttggtgct
    10261 taaatcgtgt tcataggttt tttcgttata gtcgtcaggg tagtcgttac gccatttttc
    10321 aactggcggt aaaccgcaag attgtggagt atctttcatc acaaggtaga ttggaatagc
    10381 tgcgatcatt gctgcaattc ccgggaaata aagggcttgt tgccatacat cttttgccgt
    10441 tgcttctaca ccatactcac tgaaatagag cgcacttgct aatagcacca tcgcccccgg
    10501 caccataccg cctaagttgt gagcggtatt ccagatagaa acgatagtac cacgctcaga
    10561 tttagaccac cagtgaacca ttgttcgtcc acacggtggc caacccatac cttgaaacca
    10621 gccgtttaag aagatcatta cccacattac taaaatgctt gatgttgccc aaggcattaa
    10681 gcccataagg gtcatacata gacctgaaag taacaatccg gtcggtaaaa atactttcgg
    10741 gttagagcgg tcggacattc ctgccatcac gaattttgat aaaccgtagg ccaggccggc
    10801 agctgtacca atcacaccga gatcggtttt tgtatacata ccagcttcaa tcaagccttt
    10861 ttgtgctaag tcgaagtttg cacgcacaaa atagtaagcg gcatagccga agaaaattcc
    10921 tgcaaacact tgccaacgca agaatttata tcttgcatcg actttgtccg ccggtaattc
    10981 cgcaatgtgc ggagcgggtt taaatggacc aaacatagta ttc
//
LOCUS       DANLYI010000065        11002 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.065, whole genome shotgun sequence.
ACCESSION   DANLYI010000065 DANLYI010000000
VERSION     DANLYI010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 11002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11002
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.065"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            37..1119
                     /gene="serC"
                     /locus_tag="PXD81_001249"
     CDS             37..1119
                     /gene="serC"
                     /locus_tag="PXD81_001249"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HDL5537274.1"
                     /translation="MTEIYNFSAGPAMMPKKVLEKAQQELLNWLDQGTSVMEVSHRGK
                     LFMELAAQSEADLRKLYNVPDNYRILFLQGGARGQFAAIPMNLIGKKGKALYLNSGHW
                     SATAAKEARNFAEIDEINILETEPQLKVSRLDFSDIAEQYDYIHYCTNETISGVEIFD
                     IPNVGNTPLVADMSSNILARGIDINKFGVIYAGAQKNLGPAGITIVIIRDDLIGNARK
                     DTPSIWNYATQRDADSMINTPPTFAWYLCSLVFQHLLAEGGLKATEERNLAKATLLYE
                     YLDSSTFYYNTVAHENRSLMNVTFTTGNDELNAKFVAEATACGLQALKGHKVLGGMRA
                     SIYNAMPIDGVKALIEFMKVFEQRNA"
     gene            complement(1176..2153)
                     /locus_tag="PXD81_001250"
     CDS             complement(1176..2153)
                     /locus_tag="PXD81_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HDL5537275.1"
                     /translation="MTLAVAAIIGGLIILVWSADRFVEGAASTARHFGMTPLLIGIVV
                     IGFGTSAPEMLVSASSALSGSPGIALGNAYGSNITNIALILGLTAIIKPLSVNSDVLK
                     KELPILLAVTLLSAYLVYDANVTQLDAIILLAVFFVYMAWTIITAMKSKNDALSLDVD
                     AELTNSAKMGLGKSILWLIIGLVSLVISSQFLVWGAVEVAKFFGVSDLVIGLTIVAVG
                     TSLPELASSIAAARKGEVDLALGNIIGSNLFNTLAVVGIAGAIEPMQVSAEVFSRDVL
                     MMSALTFLLFIFGINLYRRTEGGRINRLEGLLLFAAYVGYNFYLFKTAG"
     gene            2265..4832
                     /gene="glnD"
                     /locus_tag="PXD81_001251"
     CDS             2265..4832
                     /gene="glnD"
                     /locus_tag="PXD81_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HDL5537276.1"
                     /translation="MMKNSNELKTLLSAFTEQQRQAFQSENVLSLLAQRSQFYDQLLQ
                     ALWQDFGLAQYEHITLLAVGGYGREEMFPLSDLDILVLTEKPLEEAIQQRLNELFALL
                     WDSKLQLGTSVRTLEECIQIGKAEISVATNMLEGRFLLGNKSLWLRLKEALYQPDFWD
                     IKAFFRAKMAEKNERYARYHNTSYNLEPDLKHSPGGLRDLHLISWIMLRHYGVSSFVD
                     LLNKGLLYPEEYKELIAAQAVLFRMRFALHLQLKRYDNRLRFDRQLQLSEILGYQGEA
                     NQAVELMMRDYFQATKSISQLSQLLLNSFEQAYVNELQKNAQKQPLDQYFYRQEQQLF
                     VVERKIFKTQPTTMLDLFFHLTNDPTLIANADTLRQLRLCLQQQHEPMCFLPEMREKF
                     VQLFAQPKVISRAIVPMHQLGFLSAYLPQWKGIEGLMQFDLFHIYTVDEHTVRVMLNL
                     ENFSEDPSRAFFPLCHQLFATLSEKPLLYLAALFHDIGKGREGEHAQVGALDMREFAE
                     LHGFSQAQINDMVWLVAEHLTMSITAQRRDIHDPQIISEFAKKVQNQTALSSLLCLTV
                     ADICATSESLWNDWKASLFTQLYQFTLQQLAQNLNYQAVAQEHRLQALELMKFVLSAD
                     ERIALNAFWQPCPERYFLHHTPTQLVWHALHYVKTRRLPMVLISNEYARGGTEIFVCC
                     QDQPQLFARIAQLLSQKRISIHDAQIITTENGVVLDSFIVSERDGDDDLTDERCMQIQ
                     QAMMKMLSLTETGIKFTKKPVKHLSFKRKTRLRFLPPSSKQQTEFELFTLDREGLLAQ
                     IGYIFNRLRLNLINAKITTIGERVEDFFVVSNDQGKALSEQEQLELKAILITELDSE"
     gene            complement(5009..5767)
                     /locus_tag="PXD81_001252"
     CDS             complement(5009..5767)
                     /locus_tag="PXD81_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005567524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL5537277.1"
                     /translation="MITVKNLHHKIGNATILNNINLTIPNGGITALIGANGAGKSTLL
                     SLIARLQPIQQGEIHLNELNIANTASQTIARQLAILTQDNVIHSRITVKDLLLFGRYP
                     HHQGRITSHDNAIVEEALVRFDLSTFSHRFLNELSGGQRQRALIAMTFCQQTDHILLD
                     EPLNNLDMFHARELMKLLRKLTDELALTTIMVVHDINIAAAYADTIIAMKDGGIKMIG
                     TPKEIITTENLKTVFNLDAEVLECQGKPLVIHHI"
     gene            complement(5787..6663)
                     /locus_tag="PXD81_001253"
                     /pseudo
     CDS             complement(5787..6663)
                     /locus_tag="PXD81_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005607611.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
     gene            complement(6716..7684)
                     /locus_tag="PXD81_001254"
     CDS             complement(6716..7684)
                     /locus_tag="PXD81_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HDL5537278.1"
                     /translation="MFSFFRLNLFLLILLIPISASVGVADFHWSEIFHSSEQSQIFFI
                     SRLPRTLAVILVGATLAVAGMVLQIVLKNRFIEPSMIGTSQSAAIGILLASLLFPTSA
                     LLLKMSIATLSALMGMGLFMLLIRQLPPHQQLMLPLIGIVFGNVIEAISTFIAYETDS
                     LQVLSIWFSGDFSGILAGRYELLWITAILAVVVYVMADQLTIAGLGENISTNLGINYK
                     QMTWLALIVVAMITAIVVVTVGQIPFIGLVVPNIVSRIAGDRLRKNLPAVVVLGANLV
                     LICDILGRAINAPYEVPISTIFGIIGTVIFLYLLLKEKRAEEKARG"
     gene            complement(7778..8677)
                     /locus_tag="PXD81_001255"
     CDS             complement(7778..8677)
                     /locus_tag="PXD81_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005700892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HDL5537279.1"
                     /translation="MIKKALFALTATAVFAASAFAKDVTIPTARGEVTFKETPSKIAV
                     FDTGSLDTLQALGVKIDGMPEFKVLSYLKPSADKAVNVGTVFEPDLEALNSLNPDLII
                     VGTRTAKKFDEVSQIAKTIDMTDNGDKLIESGLQRLDSFGKLFNKEAETEKLKAEINT
                     LLDQTKAAVKGKGNGLIILVNGGKISAFGSGYRLSWIHDNLGIPMADSSIKNTGHGQP
                     VSFEFIQKLNPDWLFVLDRIAAIGQEGQSAQQVLDNELVRQTKAWKNGNIVYLSAASY
                     LAPGGYEQMKTDLTNIKTAFEKK"
     gene            8851..10314
                     /locus_tag="PXD81_001256"
     CDS             8851..10314
                     /locus_tag="PXD81_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HDL5537280.1"
                     /translation="MAEYSGTTQEKMKDKQVEQINISPHSIEAEQAVLGGIMLSNDHW
                     DNVAERVQPHDFYNYAHRIIFEQMAELVRHNKPIDIITLDQALKDKGVLQDVGGFAYL
                     AELSKNTPSAANILAYADIVRDRSDLRSVISAGNQIAEMGYKTKGRNSKEVLDEAERI
                     VFEIAEKRNNANEGPQRIDDILLKTLARMDTLSKNRHNGGVTGVSTGFVDLNKKTAGL
                     QPSDLIIVAARPSMGKTTFAMNLCENASLVDIEDKDDLDEQGNPKKKPAKPVLIFSLE
                     MPADQIMMRMLASLSRVDQTKIRTGQISDDEDTAKISSTMAILQERNNIYIDDSSGLT
                     PTELRSRARRIYRENGGLSLIMVDYLQLMRAPGFADNRTLEIAEISRSLKALAKELEV
                     PVVALSQLNRSLEQRADKRPVNSDLRESGSIEQDADLIMFIYRDEVYNDNSDLKGIAE
                     IIIGKQRNGPIGRVRLTFQGQYSRFDNYTGGHYDDEY"
     gene            10304..10609
                     /locus_tag="PXD81_001257"
     CDS             10304..10609
                     /locus_tag="PXD81_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HDL5537281.1"
                     /translation="MNTKPTQPITLCAIYRSKAKEGMYLYVPKRDQFEQVPETLRQLF
                     GKPEFVMMFNLTGEKPLVRAKNEEVLQLLIEQGYYLQMPPPPENLHKAFIAQQKGEK"
     gene            10609..>11002
                     /gene="nrdR"
                     /locus_tag="PXD81_001258"
     CDS             10609..>11002
                     /gene="nrdR"
                     /locus_tag="PXD81_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HDL5537282.1"
                     /translation="MRCPFCSTEETKVIDSRLAASGYQIRRRRECISCKERFTTFESA
                     ELVVPYVIKNNGSREPFDAEKLRTGLRRALNKRPVSADDVEKAISQIIIQLQAMGERE
                     VPSRHIGTLAMNALKQLDKVAYIRFASIY"
BASE COUNT         3573 a         2152 c         2251 g         3026 t
ORIGIN      
        1 gaatagaatc aatattatta ctatacgagg tgaaaaatga cagagatata taacttcagt
       61 gccggcccgg caatgatgcc gaaaaaagta ctcgaaaaag cgcaacaaga attattaaat
      121 tggctagacc aaggcacatc agtgatggaa gtcagccacc gtggaaagct atttatggag
      181 ctggcggcac aatctgaagc agatttacgc aagctgtata atgtgccgga caattatcgt
      241 attctatttc ttcaaggtgg tgcaaggggg caatttgcag ccattccaat gaatttaatc
      301 ggtaaaaaag gcaaagcgct ttaccttaat tcagggcatt ggtcggcaac agcagcaaaa
      361 gaagcacgta actttgcgga aattgatgaa atcaatattt tagagactga accgcaactt
      421 aaagtgagtc gtttagattt cagcgatatt gcagagcaat atgattatat ccattattgc
      481 accaatgaaa ccatcagtgg ggtagaaatt ttcgatattc cgaatgtcgg aaataccccg
      541 ttagtggcag atatgtcgtc taatatctta gcccgaggga ttgatatcaa caaatttgga
      601 gtaatttatg ccggagcgca aaaaaatcta gggcctgctg gcattacgat tgtgattatt
      661 cgtgatgatt taattggcaa tgcacgcaaa gataccccat ctatttggaa ttatgcaact
      721 cagcgagatg ctgattcgat gattaatacc ccgccaactt ttgcttggta tttatgttct
      781 ttagtgttcc aacatttact ggcagagggt ggtttgaaag ctactgaaga acgtaatctt
      841 gctaaagcta cattgcttta tgagtatttg gatagttcaa cattctatta caatacggta
      901 gcacacgaaa accgttcctt aatgaatgta acctttacca cgggtaatga cgagctaaat
      961 gcaaaatttg tggcagaagc gaccgcttgt ggtttacagg cattgaaagg ccataaagta
     1021 ttaggtggaa tgcgtgcctc aatttataac gcaatgccga ttgatggggt aaaagcactc
     1081 attgaattta tgaaggtatt tgaacaacgt aatgcttaat tcaagcttaa gaatataaat
     1141 taaagagcca cgtttgtggc tctttgaatt tggcactagc ccgcagtttt aaacaaatag
     1201 aaattatagc ctacataagc ggcaaagagt aataagccct ctaaccgatt aattctgcca
     1261 ccttctgtcc gacgatagag gttgatcccg aaaatgaata ataagaaggt gagagccgac
     1321 atcatcaaga catcgcgaga aaacacttcc gctgaaactt gcattggttc tatagcaccg
     1381 gcaatcccca ccacggctaa cgtattaaat aagttagaac cgataatgtt acctaaagct
     1441 aaatccactt ctcctttacg agcagctgca atagaagagg ctaattcagg tagtgaggtg
     1501 cctaccgcca caatagtaag cccgattacc aaatcactca caccaaaaaa tttagccact
     1561 tcaactgctc cccagactaa gaattgggaa ctaattacta gcgatactaa accaataatt
     1621 agccaaagaa tggattttcc taatcccatc ttagcactat tggttaattc tgcatcaaca
     1681 tctaagctta atgcatcatt tttacttttc attgcggtaa taattgtcca cgccatatat
     1741 acaaaaaaca cagcaagtaa aataatcgca tcaagttgag tgacgtttgc atcataaacc
     1801 aaataagcag aaagcagggt gacagccagt aaaatcggca actctttttt taacacatcg
     1861 gaattaactg atagtggttt aattattgcg gttaaaccta aaatcagcgc aatattggta
     1921 atgttagaac cgtaggcatt accgagagcg atacccggcg atccgcttaa tgcagaagat
     1981 gcagatacga gcatctccgg tgcagatgta ccaaaaccaa tgaccacaat accgattaac
     2041 aatggtgtca taccaaaatg gcgagccgta gaggctgccc cttccacaaa acgatccgca
     2101 ctccaaacta atataattaa gccaccgata atagcggcaa cagcaagtgt cataaactat
     2161 ccttttagat gaaaaacata aaggcattat aagcatttta gccaaaataa atataggcaa
     2221 acgaaaataa atgacctatt attttgccag tttaggaaga gattatgatg aaaaacagca
     2281 acgaattaaa aacgctttta tcagcattta cggagcaaca gcggcaagca tttcaatccg
     2341 aaaatgtact cagcttactt gcacaacgta gccaatttta tgaccaacta ctgcaagcac
     2401 tttggcaaga ttttggactc gctcaatatg aacatattac cctattagcg gtaggggggt
     2461 acggacgtga ggagatgttt ccgctttcgg atctggatat cttagtgtta actgaaaaac
     2521 cgctagaaga ggctattcag cagcgtttaa acgaactatt tgccttgctt tgggacagca
     2581 aactgcaact tggcaccagt gtgcgtacgc tcgaagaatg tattcagatt gggaaagcag
     2641 aaatttcggt tgccactaat atgttggaag ggcgattttt attgggtaat aaatcgcttt
     2701 ggctaagatt aaaagaagca ctttatcagc cggatttctg ggatattaaa gcgtttttta
     2761 gagcgaaaat ggcggaaaaa aatgagcgtt atgcacgtta tcataatact agctataacc
     2821 ttgagccaga tctcaaacat agcccgggag gattgcgaga tctgcattta atctcttgga
     2881 ttatgctacg gcattatggt gtatcttctt ttgtcgatct cctcaataaa gggctacttt
     2941 acccggaaga atataaggag ctgatcgcag cacaagcggt actttttcgg atgcgttttg
     3001 cacttcattt gcaactgaaa cgttacgata atcgccttcg gtttgacaga caattacaac
     3061 tgagtgaaat attaggctac caaggcgaag caaatcaagc ggtggaatta atgatgcgag
     3121 attattttca agccacaaaa tctatttcgc aactgagcca attattgtta aacagttttg
     3181 aacaagccta tgtaaacgag ttgcaaaaaa atgcacaaaa acaaccgctt gatcagtatt
     3241 tttatcggca agaacagcaa ttgtttgtgg tcgaacgcaa aatatttaaa acccaaccca
     3301 caacaatgtt ggatctcttt tttcatctta ctaatgaccc tacgttaata gcgaatgccg
     3361 acaccttacg tcagttgaga ctgtgtttac agcaacaaca cgagcctatg tgctttttgc
     3421 ctgaaatgcg agagaaattc gtacagcttt ttgctcaacc gaaagtcata agccgagcaa
     3481 tagttccaat gcaccaactg ggttttttaa gtgcttatct accgcaatgg aaggggatcg
     3541 aagggctaat gcaattcgac ctattccata tttatacagt cgatgagcat acggtcagag
     3601 tcatgttaaa tttagaaaat tttagcgaag atccaagtcg ggcgtttttc ccactatgtc
     3661 atcagctatt tgcaacgctt tctgagaaac cgttgctcta tcttgcggct cttttccacg
     3721 atattggcaa gggtagagag ggcgaacacg ctcaagttgg tgcgctggat atgcgagaat
     3781 ttgcggaact acacggtttt tctcaagcac aaatcaatga tatggtttgg ctggtggcag
     3841 agcatttgac aatgtcgatc accgcacaaa gacgagatat ccacgatccg caaatcatca
     3901 gcgaatttgc aaaaaaagtg caaaatcaga ccgctttatc ttcactactt tgtttaacgg
     3961 ttgcagatat ttgtgcaacc agtgagagcc tctggaatga ttggaaagcc tcgctgttta
     4021 cccaattata tcaatttacg ctacagcaac tggctcaaaa tttgaattat caagcagtgg
     4081 cacaagagca tcgcttgcaa gcattagaac tgatgaagtt cgtgctttca gctgatgagc
     4141 gaatagcatt aaacgcattt tggcaacctt gcccggagcg ttattttctt caccatacac
     4201 cgactcagtt ggtatggcac gctctgcatt atgtaaaaac tcgccggttg ccgatggttt
     4261 taatcagtaa tgaatatgcc cgtggtggta cagaaatttt tgtatgttgc caagatcaac
     4321 ctcaactgtt tgctcgaatc gcccaattac tcagccaaaa acgcattagc attcacgatg
     4381 cacaaattat tactactgaa aatggtgtag tgttagacag ctttattgta agtgagcgtg
     4441 atggtgatga tgatttaacc gatgagagat gtatgcaaat tcagcaggcg atgatgaaaa
     4501 tgttaagctt aactgaaaca ggcataaaat ttaccaaaaa accggtgaaa catttgtcat
     4561 ttaagcgtaa aactcggctg cgttttcttc ctccgtcatc aaagcaacaa accgaatttg
     4621 aactttttac cttggataga gaagggttgt tggcacaaat aggctatatt tttaaccggc
     4681 tccgattaaa tttaattaat gcgaaaatta cgactattgg tgaaagggta gaggatttct
     4741 ttgtggtcag taatgatcaa ggaaaagctt tatcggaaca agaacagtta gaactaaaag
     4801 ccattctaat taccgaacta gatagtgaat gaaaacaacc gcttggagtg aagactgcaa
     4861 gcggtaaatt tttcaacgtt ttaggtaaat taaatcacga gttaagagag gttgagaatt
     4921 caagaaatta gaaactaaat ataagtttct atcgtttaga ttaataaatt aagacgcaat
     4981 ttttattaat taccatgatc ttcaaacgtt aaatatgatg aataactaaa ggtttgcctt
     5041 ggcattctaa cacttcggca tcaagattaa acactgtttt taaattttca gtggtgataa
     5101 tctcttttgg ggtgccgatc atcttaattc caccatcttt cattgcaata atagtatctg
     5161 cgtaagcagc agcaatgttg atgtcgtgta ccaccattat agtagttaag gcgagttcat
     5221 cggttaattt tcgtaataat ttcattaatt cccgagcatg gaacatatct aaattattca
     5281 gtggttcatc taataagata tgatcggttt gctgacagaa ggtcattgca attaatgccc
     5341 gctggcgttg ccctccggat aattcattta agaatcggtg ggagaaagtc gataaatcaa
     5401 atcgtaccag agcctcttct acaatggcat tatcgtgcga agtgattctg ccttgatgat
     5461 gagggtaacg cccaaataac agcagatctt tgacggtaat acggctatga atcacattat
     5521 cttgggtcaa gatagccagt tgtcgagcga tagtttgaga agctgtgttg gctatattca
     5581 gctcatttaa gtggatttca ccctgttgga taggttgcaa gcgtgcaatt aaagagagca
     5641 gggtggattt gcccgcacca tttgctccga ttaaagcggt aataccacca tttggaatgg
     5701 ttaaattgat attattcaga atcgtggcat tcccgatttt gtggtgaaga tttttaaccg
     5761 ttatcatttt cttccttaat ttatggttat tttttccgtt ttaaaatgag ataaataaag
     5821 acaataccgc caataaattc aataacgaca ctcagaaccc cttgcatttt cagcacttgc
     5881 tcgaagattg cctgccctaa aattaaggtg atggcagaga ttaaaaaagt cattggtatt
     5941 cgcacgctat ggcaaacagt cgggctaatt gcattaacaa tggcacaaac aagtagccct
     6001 agaaatagaa tcgggccaac aagtgcggtt gaaacagaca ccaacaatcc acaacagatt
     6061 agtaattgac gtgaaaattg atgatattct aacccaagcc caattgcctt atctttgcca
     6121 agtaacaaaa tatctaattt atgccgttgt aaccagaccc aaatggcaga gagaatagcg
     6181 atacagcctc caatccaaag taatgtgggg gtagtcaggt taaatgaggc aaagaagagc
     6241 cttgagcaac ggcaaactct gttgggtcga cattcgttgc aacaaattat ttaggcttct
     6301 aaataagaca ccaaagatga caccgattaa aagcattcgg gttaaatcgg atcgatttcg
     6361 attcagtgaa ctaaatagta atattgatgc ccctagcatt aacaaggttt caaagaaaaa
     6421 cttatagctt gggtctaact gagtaaagcc taaaccgcca aataaaaata ctaacgaggt
     6481 ttgcaatagc aagtagagcg aatcgaaccc caaaatactt ggagttaaaa taggattatg
     6541 tgtgagtgtt tggaatagca gggtcgaaat accaatcgta taagcaatag tacagagcaa
     6601 aataagcttt ttgccacgca gttgcaatat aaagtcccaa ttacctttcg cattataggt
     6661 catataaaac agaatacttg ctaataagat taagcttaaa acgataaagc gtgatctaac
     6721 cacgagcctt ctcctcagcc cgtttctctt tgagcaataa atagaggaaa atcactgtcc
     6781 caataatccc aaaaatagta gaaatcggca cttcataagg ggcattgatg gctcgcccta
     6841 aaatgtcaca aatcaggact aaattcgcac ctaacaccac gaccgcaggc agatttttac
     6901 gcaatcggtc acctgctatg cgagagacaa tattagggac gaccaagccg ataaacggaa
     6961 tctgtccaac cgtcaccacg acaatagcgg taatcatcgc caccacaatt aaggcaagcc
     7021 aagtcatttg tttgtagtta atgcctaagt tggtactaat attttcacca aggcctgcaa
     7081 tggttaattg atctgccatc acataaacca caacagccag aatagcggta atccagagca
     7141 gttcataccg acctgccaaa ataccggaga agtcgccgga aaaccaaatc gaaagcactt
     7201 gtaggctgtc agtttcataa gcaataaaag tgctgatagc ttcaataaca ttgccaaaaa
     7261 cgatcccgat taatgggagc attaattgtt gatgaggcgg taattgacga atgagcaaca
     7321 taaaaagccc cattcccatt aatgcactta atgtggcaat agacattttt aacagtaatg
     7381 ctgatgttgg gaaaagtaaa ctagcaagta aaataccaat tgccgcactt tggctagtgc
     7441 caatcatact aggctcaata aagcggttct tcagcacaat ttgtaatacc atacctgcaa
     7501 cggctaaggt tgcaccgact aatatcactg cgagcgttct tggtaaacgg ctgataaaaa
     7561 agatctgact ttgttcggaa gaatgaaaaa tttcactcca atgaaaatct gccacaccca
     7621 cactggcact gattggaatg agcaatatta aaagaaatag atttaatcta aaaaaagaga
     7681 acatagtgat aaatagcaag cggtcatatt ctctctcttt tttacaaatg caggcacttg
     7741 caaaaatagg aggaaatctg accgcttgta aggttaatta ttttttctca aatgctgttt
     7801 taatgttagt taggtcggtt ttcatctgct cataaccacc cggcgctaaa tatgaggcag
     7861 cacttagata aacgatatta ccgtttttcc aagctttagt ttgacgaact aattcattat
     7921 ccaacacttg ttgggctgat tgaccttctt gtccgatagc cgcaatacgg tctaacacaa
     7981 acaaccaatc cggattgagt ttttggataa attcaaacga aacaggctga ccgtgaccgg
     8041 tatttttaat gctagaatct gccattggaa tccctaaatt atcgtgaatc caacttaaac
     8101 gataacctga accaaatgca gaaattttgc caccattgac taaaataatc agtccgttac
     8161 ctttaccttt tactgctgct ttggtttggt ctaataaagt attaatttct gcttttagtt
     8221 tttcggtttc cgcttcttta ttaaataatt tgccgaaaga atcaagacgc tgtaatccgc
     8281 tttcgattaa tttatcgcca ttatccgtca tgtcgatggt tttcgcaatt tggctgactt
     8341 catcaaattt ttttgctgtg cgtgtgccaa caatgatcaa atccggattt aaagaattta
     8401 acgcttctaa atccggctca aataccgtac cgacattcac cgctttgtct gcactcggtt
     8461 taagataaga aagcacctta aattcaggca taccgtcaat ttttacacct aaggcttgta
     8521 aagtatccaa acttccggtg tcaaacacgg caattttaga cggggtttct ttgaaagtaa
     8581 cctctccacg tgcagttgga atagtaacat ctttggcaaa tgcagaagca gcaaacacag
     8641 cagttgcggt caatgcaaac aaggcttttt taatcattgg ataactccat ttatattgaa
     8701 aataattctc aaagatttta acgaaaattg attttaagta aagcggaaaa taacaagcgg
     8761 gcgaattttg cctaaaattt gcaaatttca atttaattaa aattccgata cactatgtac
     8821 atcaatacgt cattatagca agagaaaaat atggcagaat atagcggaac aacccaagag
     8881 aagatgaaag ataagcaggt tgagcaaatt aatatctcac cacattcgat tgaggcagag
     8941 caagcggtgc ttggtggcat tatgctcagt aatgatcact gggataatgt tgctgagcgt
     9001 gtacaaccgc acgattttta caactatgcg catcgaatta tttttgaaca aatggcggag
     9061 ttggtgcgcc acaataaacc gattgatatt attacgctgg atcaagcctt aaaagacaaa
     9121 ggggtgctgc aagatgtagg aggttttgcc tatttagccg agctttctaa aaataccccg
     9181 agtgcagcaa atattttagc ctatgcggat attgtgcgag atcgttccga tttaagaagt
     9241 gtaatcagtg ccggcaatca aattgcggaa atgggctaca aaacgaaagg gcgtaactcc
     9301 aaagaagtgt tagacgaggc agagcgaatt gtgtttgaaa ttgcagaaaa acgcaataac
     9361 gccaatgaag gtccacaacg tattgacgat attttgctta aaacactcgc tcgaatggat
     9421 acgctatcta aaaatcgcca taacggcgga gttactggtg tttctaccgg gtttgttgat
     9481 ctcaataaaa aaaccgcagg gttacagcct tcggatttaa tcattgtggc agcccgtcct
     9541 tcaatgggga aaactacttt tgcaatgaac ttatgtgaaa atgcctcatt agtggatatt
     9601 gaagataaag atgatttaga cgaacagggt aatccgaaga aaaaaccggc aaagccagtg
     9661 ctaattttca gcctggagat gccggcagac caaattatga tgcgtatgct tgcctcactt
     9721 tctcgggtcg atcaaaccaa gatccgaacc ggtcagattt cagatgatga agacactgct
     9781 aaaatctcca gcacaatggc aattttacaa gaacgtaata atatctatat tgatgatagc
     9841 tccggtttaa caccgaccga attacgttct cgagcgagac ggatttatcg tgaaaatggc
     9901 ggattaagct tgattatggt ggactatctc caattaatgc gagcgccggg atttgcagat
     9961 aatcgtactt tagaaatcgc agaaatttcc cgttctttaa aggcactagc gaaagaatta
    10021 gaagtgccag tagtcgctct ttctcagcta aaccggagtt tggaacaacg tgccgataaa
    10081 cgtccggtaa actcggattt gcgtgagtca ggttctattg agcaagatgc agacttaatt
    10141 atgtttattt atcgtgatga ggtctataac gacaattccg atctgaaagg cattgcggaa
    10201 attattatcg gaaaacaacg taacggcccg attggtcggg tgcgtttaac gttccaaggg
    10261 cagtattccc gttttgataa ttacacagga gggcattatg atgatgaata ctaaaccaac
    10321 ccagccaatc acactttgtg cgatttatcg cagtaaggca aaagaaggaa tgtatttata
    10381 tgtgcctaag cgtgaccaat ttgagcaagt gccggaaaca ttacgccagt tatttggtaa
    10441 gcctgaattt gtgatgatgt ttaatttaac cggtgaaaag ccattggtgc gtgctaaaaa
    10501 cgaagaagtg ttacaattac ttatagagca aggctactat ttgcaaatgc cacctccgcc
    10561 ggagaatttg cataaagcct ttatcgcaca acagaaaggg gaaaaataat gcgctgccca
    10621 ttttgttcca ctgaagaaac taaagtgatc gattcacggc ttgcagcaag cggttatcaa
    10681 attcgccgcc gccgtgaatg tataagctgt aaagaacgtt ttactacctt tgaatcggcc
    10741 gaattagttg tgccttatgt gatcaaaaat aatggtagcc gagagccgtt tgatgccgaa
    10801 aaattaagaa cagggttaag acgtgcatta aataaacgcc ctgtcagtgc tgatgatgtc
    10861 gaaaaagcaa ttagccaaat cattattcag ctgcaagcga tgggcgaacg agaagtgccg
    10921 agtcgccata tcggtacatt ggcaatgaac gcacttaaac aattagacaa agtagcatat
    10981 atccgctttg cctctattta tt
//
LOCUS       DANLYI010000066        10951 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.066, whole genome shotgun sequence.
ACCESSION   DANLYI010000066 DANLYI010000000
VERSION     DANLYI010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 10951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10951
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.066"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            72..818
                     /locus_tag="PXD81_001259"
     CDS             72..818
                     /locus_tag="PXD81_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005616080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDL5537283.1"
                     /translation="MQLRHKQIMEYIQQFDEANVQELAEHCGVSIETIRRDLNKLDKD
                     GLLHRTHGGAVSHKKRDIGRSFTTRQRLNSEAKRSIAENVLEYLQPESVIALDASSTS
                     WNVAQRIPNIPCTVVSSSMRIIYSLSHKPHIKTIATGGIYSEKYDAFYGPLSEQLLTR
                     LKIDLAILSCTGIADGAIWESNEINISLKRKLIANSKQVFLLADHSKFERKDLFRIED
                     LSCIDKLFINQPPPDFLKNYCLAHQIEIII"
     gene            1014..2777
                     /gene="fucI"
                     /locus_tag="PXD81_001260"
     CDS             1014..2777
                     /gene="fucI"
                     /locus_tag="PXD81_001260"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005619331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HDL5537284.1"
                     /translation="MTTLKSTPVKIGIRPTIDGRRLGVRESLEDQTMNMAKAVAQLIQ
                     SEVRHPNGDFVECVIADSTIGGVAEAAACAEKFKRENVGAVITVTPCWCYGSETIDMD
                     PHMPKAIWGFNGTERPGAVYLAAALAGHSQLGLPAFSIYGTEVQEADDQSIPDDVREK
                     LLRFARAGLAVATLRGKSYLSIGSVSMGIAGSIVNQQFFQEYLGMRNEYVDMTEIKRR
                     LDRHIYDAEEFKLAMSWVKTYCKEGIDVNAPENQRSPEEREKLWEDVVKMTIIARDLM
                     VGNPRLAELGYSEEALGHNAIAAGFQGQRQWTDHLPNGDFMEATLNSTYDWNGVRAPY
                     IMATENDSLNGVNMLFGNLLTGQAQIFADVRTYWSEDSVERVTGWRPESGFIHLINSG
                     SAALDGTGQHTDRAGKPTIKPAWEVTEEDGKRCLENTCWCPAVHEYFRGGLSSQFLTK
                     GGMPFTMHRINLIKGIGPVLQIAEGWSIDLPENVHDILNKRTNETWPTTWFVPRLTGK
                     GAFTDVYSVMANWGANHCVATFGHVGADLITLASMLRIPVCVHNVEDKDVFRPSAWNG
                     FGQDKEGQDYRACANFGPLYK"
     gene            2971..4404
                     /gene="fucK"
                     /locus_tag="PXD81_001261"
     CDS             2971..4404
                     /gene="fucK"
                     /locus_tag="PXD81_001261"
                     /EC_number="2.7.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009874965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
                     /protein_id="HDL5537285.1"
                     /translation="MPISLIFDCGATNLRTIAINQAGEIVAAHHLPNNTQPDVESPEF
                     HIWDIEEIWSKLMTCAKQTLSQLSEQQRQEIVGISVTTFGVDGALFDKNGKQLYPIIS
                     WKCPRTLPIMENLANYLDVEALYQRNGVGHYSFNTLFKLLWLKENKPEIYQQAESFLF
                     ISSILTYLLTGVQTTDRTMAGTSMMTNIENDSWDKTVLDLLGLTENHFPPMKSAGEVV
                     GTLKGEISQELGLSGQIPVISCGHDTQFAIFGSGAGYNQPVLSSGTWEILMVRTPKAK
                     PQWQFVQNGLTIEFDSQSGYFNPGVQWVASGVMEWFGKRFFSDIANTEHYYRTMIEEA
                     AEVPAGSNGVKLVGNFDGTTAKTGSIIGLSMHITRGEIYRAGLEYMAHRLKEGLDVLQ
                     EVGHFNAESLICVGGGSKNALWNQIRADVLNRPIDVVDFPESTVLGAAMFTFTGAGVF
                     ENPNAAQQAMKPNVKRVEPSSNSQFYK"
     gene            4479..4910
                     /gene="fucU"
                     /locus_tag="PXD81_001262"
     CDS             4479..4910
                     /gene="fucU"
                     /locus_tag="PXD81_001262"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012341412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HDL5537286.1"
                     /translation="MLKGIHPAISPDLLKVLAEMGHGDELVLSDAHFPAHSIHQNVIR
                     ADGLSVATLLEGISALFEFDQYVEAPLAMMQAVKGDTLDPSVEERYLNAIKKVNGTAP
                     KIERVERFAFYDRAKNAYAVVITGELAKYGNIIIKKGVTPV"
     gene            4970..5623
                     /locus_tag="PXD81_001263"
     CDS             4970..5623
                     /locus_tag="PXD81_001263"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HDL5537287.1"
                     /translation="MDRKILARQIIDTCLEMTRIGLNQGTAGNVSVRYKDGMLITPTG
                     TPYEQMTEDSIVFVDGNGKHEEGKLPSSEWQFHLAVYEARPELNAVVHNHAKNCAAVS
                     ILGEPIPAIHYMIACTGTNHIPCVPYAPFGTHQLADYVREGIKHSKAILLAHHGLITA
                     DKTLEKALGIAHEVEVIAEWYLKLLATGKPIPTLNKEQMDIVLGKFKSYGSWVESEQ"
     gene            5685..7214
                     /locus_tag="PXD81_001264"
     CDS             5685..7214
                     /locus_tag="PXD81_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HDL5537288.1"
                     /translation="MQNEVILTMKNISKSFAGVNALKGVELTLRKGEVHALMGENGAG
                     KSTLMKCLLGVYQADEGEIIYKGQPVRFESVLEAQKAGISMIFQELNLIPHLTVAENI
                     FFAREPLKHGLVDKDKMVEESAKLLNFFEIDVDPNDEVRMLSVAKQQMVEIAKALSFD
                     VEVLIMDEPTSALTEKEIDKLFELVDRLRAKGVSIVYISHRMEELKRICNHITIFRDG
                     TYVSNHKFNEITMDEIITKMVGRSLDNHFPPKTAVVTNDIILSIMNAERKGVFEPLNF
                     DLRKGEILGITGLVGAKRTELARAIFGADPLDSGEIFVHENKVSIKDPSDAIKAGIAY
                     LSEDRKLNGVAVRMSIRENITMASMDKVANQIGVISYDEEEKASKTFIDKMEIKTPTI
                     EQKVQNLSGGNQQKVVIGKWLFREAKVMIFDEPTRGIDVGAKYAIYQLLDELAANGVG
                     VIVISSELPEVLGVSDRIIVMREGRMTGMLETKKTNQEEIMHYATGVKNMFAREYGVE
                     K"
     gene            7211..8209
                     /locus_tag="PXD81_001265"
     CDS             7211..8209
                     /locus_tag="PXD81_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL5537289.1"
                     /translation="MNEKQKEMLRKMAALAGLVLLIIFFSVTNEYFFTSNNIMTVGLQ
                     TSTIALIGIGATCVILTGGIDLSTGSVVALSGVAAAMIVNAGVPVPLGMILGILVGGI
                     CGLINGILVTRMKLPPFIATLGMMMVARGLALYVTNAAPVSGMPESFANLGNGALFKI
                     VEEGANGLPKVVFAGIPYPVIIMIFITVLFTFALSKLKVGRYLYAIGSNEEAARLSGI
                     KTNIVKIYAYVASGLLSGLTGVILASRLVTAQPNGGVSYELDAIASAVVGGTSLMGGV
                     GTIPGTLIGSFIIGVLRNGLNMNGVSSFVQMIVIGLVIIVAVSLDQLRQSKKAGKK"
     gene            8233..9180
                     /locus_tag="PXD81_001266"
     CDS             8233..9180
                     /locus_tag="PXD81_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL5537290.1"
                     /translation="MKKSALFTATLFGLSTLFAGSAMAKSDEIAVIVKSANSTFWQNV
                     RKGAETAGKDIGGQYKVTFQGPEAETAIDAQVNMVDNAVNRGVAGIVLAASDPVALVP
                     AVKKAYESGIPVVLIDSGINSDGKYYQSFLATDNRAAGKLAAEKLLAKVKGGKVAVMS
                     FTQGAASAIERTGGFIDEIKAKADYKLVGPYYSDSDMTKALNQTEDVLGSNPDLAAIF
                     GANEPTAVGMARAVKQKGFAGKIVAVGFDGNSDLQNFVRDGTLDGIVVQSSYQMGYKG
                     VDTIGKLIKGEKVEKVIDTGVVYVTKENIDSEEAKGVLY"
     gene            9236..9487
                     /locus_tag="PXD81_001267"
     CDS             9236..9487
                     /locus_tag="PXD81_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIB"
                     /protein_id="HDL5537291.1"
                     /translation="MSNTLTDKALVVIAALGGRENIVDVYACITRLRVEVKNTDIVEW
                     DKIKSVGALGVVTSGSVIQSIFGSDAEAYRDEIKRILNI"
     gene            9616..10767
                     /gene="fucO"
                     /locus_tag="PXD81_001268"
     CDS             9616..10767
                     /gene="fucO"
                     /locus_tag="PXD81_001268"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HDL5537292.1"
                     /translation="MANRLILNETSYHGKGAIQNIVHEVTSRSFKKALVVTDKDLIKF
                     NVAQKVTALLDAAGLPYEIFDEVKANPAVDVIKAGVAKFKASSADYLIAIGGGSPIDS
                     AKAIGIIINNPEFSDVLSLEGVAPTHQKCVPIIAIPTTAGTAAEVTINYVITDEAKKR
                     KFVCVDVHDVPAVALVDPDMMESMPKSLAAATGMDALTHAIEGYITKAAWELTDALHL
                     KAIELIGRSLRGAVEKNPKDVEAMALGQYVAGMGFSNVGLGAVHSMAHPLSAYYDTPH
                     GIANAVLLPYVMEFNKNYTGEKYREIARAMGVKGVDEMSQEEYRNAAIAAVQQLSQDV
                     GIPPKLHQIGVKEADLAALSVDAFNDVCTGGNPRDCTAEELLEVYRIAY"
BASE COUNT         3286 a         2277 c         2488 g         2900 t
ORIGIN      
        1 caaatttgct tgctcgacag taacgctaca agcggttaaa tttggagaaa aatttacaaa
       61 aaggatagat tatgcagctg cgacacaaac agataatgga atatatacaa cagtttgatg
      121 aagctaacgt tcaagaattg gcggaacatt gtggggtttc gattgaaact atccgccgag
      181 atttaaataa gctagataaa gatggattat tacatcgcac tcacggtgga gctgtgagcc
      241 ataaaaagcg agatattggt cgctcattta ccacccgaca acggctgaac agtgaggcga
      301 aacgcagtat cgcagaaaat gtgttggaat atttacaacc agaatcggtg attgctttag
      361 atgccagctc aacctcttgg aatgtggcac agcgtattcc aaatattcct tgcaccgttg
      421 tcagcagttc aatgcggatt atctacagcc tttcacacaa accgcacatt aaaacgattg
      481 ccaccggtgg tatttattcc gaaaaatacg atgccttcta tggtccatta tccgagcagt
      541 tactgacccg tttaaaaatt gatcttgcca ttctttcttg tacaggcatt gccgatgggg
      601 cgatttggga atcaaatgaa attaatattt cccttaaacg taaattaatt gcgaacagta
      661 aacaggtttt tttattagcc gatcacagta aatttgagcg taaagacttg tttcgtatcg
      721 aagatttatc ttgcattgat aaattattta tcaaccaacc cccaccggac tttctcaaaa
      781 attattgctt agcacatcaa attgaaatca ttatttagtg atttctgccc gattttattc
      841 agaaataaaa caaatcgggc attctttttt cactcatttt taccaaaatc ccccattttt
      901 tgtgatcgca ctcacaaaat ttaaactaaa ttcgcatttt tcttcataac tcatattgcg
      961 tatgcataat aaattcaatc gcaaattttt ttaaccaatc gggagaacta tttatgacaa
     1021 cgttgaaatc tacccctgta aaaattggta tccgcccaac cattgacggt cgccgtttag
     1081 gagtgcgtga atcacttgaa gatcaaacaa tgaatatggc aaaagccgtt gcacaattaa
     1141 ttcaatctga agtccgccac ccaaatggtg atttcgttga atgtgtgatt gctgattcca
     1201 ccatcggtgg tgttgccgaa gctgcggctt gtgcagaaaa attcaaacgt gaaaatgtcg
     1261 gagcggtgat taccgtaacc ccttgctggt gctacggttc tgaaaccatt gatatggacc
     1321 cacatatgcc gaaagccatt tggggcttta acggaacaga acgcccaggt gcagtttatt
     1381 tagctgctgc tttagcaggt catagccaat taggtttacc ggcattctcc atttatggca
     1441 cagaagtaca agaagctgac gatcaatcta ttccagacga tgtgcgtgaa aaattactcc
     1501 gctttgcccg tgccggctta gcagtggcaa ccttacgagg aaaatcatat ttatctatcg
     1561 gctctgtttc aatgggtatt gccggttcta tcgtaaacca acaattcttc caagaatatt
     1621 tgggtatgcg taacgaatat gtggatatga ccgaaattaa acgccgttta gatcgccata
     1681 tttacgatgc cgaagaattt aaattagcaa tgagctgggt gaaaacctat tgcaaagaag
     1741 gtattgatgt caacgcaccg gaaaatcaac gcagcccgga agagcgtgaa aaactctggg
     1801 aagacgtggt gaaaatgacc attattgccc gtgatttaat ggtgggtaat cctcgcttag
     1861 cagagctagg ctatagtgaa gaagccttag ggcataatgc gattgctgcc ggtttccaag
     1921 ggcaacgcca atggacagac catttgccaa acggcgattt tatggaagct accttgaatt
     1981 cgacctatga ctggaacggc gttcgcgcac catacattat ggcaactgaa aacgacagct
     2041 taaatggcgt gaatatgttg ttcggtaact tattaaccgg tcaggcacaa atctttgccg
     2101 atgtgcggac atactggagc gaagactcgg tagaacgtgt caccggctgg cgtccggaaa
     2161 gcggttttat ccacttaatc aactccggct ctgcggcgtt agacggaaca ggtcagcata
     2221 ccgatagagc aggcaaacca actattaaac cggcttggga agtgactgaa gaagacggta
     2281 aacgttgttt agaaaacacc tgttggtgtc cggcagttca cgaatatttc cgtggtggtt
     2341 tatcttccca attcttaacc aaaggtggaa tgccgtttac gatgcaccgt atcaacctga
     2401 tcaaaggtat cgggccggtt ttacaaattg ccgaaggttg gtctatcgac ttgccggaaa
     2461 atgtacacga cattttaaac aaacgcacca atgaaacctg gccgaccact tggttcgtac
     2521 ctcgcttaac cggaaaaggc gcatttaccg atgtgtatag cgtaatggcg aactggggtg
     2581 caaaccactg tgtggcaacc ttcggacacg ttggggcaga cttaattacc ctcgcctcaa
     2641 tgctccgcat tccggtttgt gtgcacaatg tggaagataa agacgtattc cgcccaagtg
     2701 cgtggaacgg tttcggtcaa gacaaagaag gtcaagacta ccgtgcttgt gctaacttcg
     2761 gaccattata taaataatac acaatcaccc tctccccttg tgggagaggg acagccaagc
     2821 aattcgttca gaattgaatg gcagggagag ggctacaagc ggtcgctttt ctctcaaaat
     2881 ttacaaatcc ctctctctga ttaaattgct aaacgcaatt taatctatct ctctaaaagg
     2941 gagatattag gataagtaaa ggagataaca atgccaattt ctcttatttt cgactgtggt
     3001 gccaccaacc tccgtacgat tgcgattaat caagcgggtg aaattgtggc agcccatcat
     3061 ctaccgaata acacacaacc agatgtggaa agcccagaat ttcatatttg ggatattgaa
     3121 gagatctggt cgaaattaat gacctgtgcc aaacagacgc tttctcaact ttcagaacaa
     3181 caacggcaag agattgttgg gatttcagta accacctttg gcgtggacgg tgcattattt
     3241 gacaaaaacg gcaagcagct ttatccgatc atttcttgga aatgcccgag aaccctaccg
     3301 attatggaaa accttgccaa ttatcttgat gttgaggcgt tatatcaacg taatggcgtt
     3361 ggacattata gtttcaacac cttattcaaa ttattgtggt taaaagagaa caaaccagaa
     3421 atttatcaac aagctgagag tttcttattc atctcatcta ttttgactta ccttcttaca
     3481 ggcgtgcaaa caaccgaccg cacaatggcg ggaacgtcaa tgatgacaaa catcgaaaac
     3541 gattcgtggg ataaaacagt tttagattta ttaggcttaa ccgaaaacca ttttcctcca
     3601 atgaaaagtg caggagaagt tgtgggaaca ttaaaaggag aaatttccca agagttaggt
     3661 ttaagcggtc aaattccagt aatttcttgc ggacacgata ctcaatttgc catttttggc
     3721 tcaggagctg gctataacca acctgtgtta agttcaggca cttgggaaat tttaatggtt
     3781 cgtaccccaa aagcaaaacc acaatggcaa ttcgttcaaa acggcttaac cattgagttt
     3841 gatagccaat ctggttattt caacccaggt gtgcagtggg tagcttcagg tgtgatggag
     3901 tggtttggca aacgtttctt ctctgatata gccaatactg aacattacta ccgcacaatg
     3961 attgaagaag cggcagaagt gcctgctggc tcaaacggtg tgaaattagt cggcaatttt
     4021 gatggcacaa cagctaaaac aggttcaatt atcggcttat ctatgcacat cactcgtggc
     4081 gaaatttacc gtgcaggctt ggaatatatg gctcatcgtc taaaagaagg gttagatgta
     4141 ttacaagaag tcggtcattt taatgctgag agtttgattt gtgtgggagg tggttctaaa
     4201 aacgctttat ggaatcaaat tcgagccgat gtcttaaata gaccgattga tgtagtggat
     4261 ttccctgaaa gtaccgtatt aggcgcggca atgtttactt ttacaggtgc tggtgtattt
     4321 gaaaatccaa atgcggctca acaggcaatg aagccaaatg tgaaacgggt tgagccatcg
     4381 tcaaatagtc aattttataa ataaagtttc tcccctctct ctgatttgag gttcattcag
     4441 aacatcaaat cagagagagg gtaaaaaagg agttcaaaat gttaaaaggt attcacccgg
     4501 caatttctcc agatttatta aaagtcttag ccgaaatggg gcacggcgat gaattagtgc
     4561 tttctgatgc acacttccct gctcactcta ttcatcaaaa cgtcattcgt gcagatggct
     4621 taagtgttgc caccttatta gaaggcattt ctgctctgtt tgaatttgac caatacgttg
     4681 aagccccact tgcaatgatg caagcagtaa aaggcgacac gctcgatcca agcgttgaag
     4741 agcgttacct caatgcaatt aaaaaagtaa acggaacagc cccgaaaata gagcgtgtag
     4801 aacgcttcgc tttctatgat agagccaaaa acgcttatgc cgttgtcatt acaggcgagt
     4861 tagccaaata cggcaacatt attatcaaaa aaggcgtaac accggtttaa ttacaagcgg
     4921 ttaaaaaagc agaatttttt acaaatataa aattaaaagg agttcaacaa tggacagaaa
     4981 aatactggcg agacaaatca ttgatacttg tcttgaaatg acccgaattg ggttaaatca
     5041 aggtacagca ggcaatgtga gcgtacgtta taaagacggt atgctcatta cgccaacagg
     5101 tacaccttat gagcaaatga ccgaagattc tatcgtcttt gttgatggca acggcaaaca
     5161 cgaagaggga aaactccctt caagtgagtg gcagttccat ttagcggttt atgaggctcg
     5221 ccctgaatta aatgcggtag ttcacaacca cgctaaaaac tgtgccgcag tgtctatttt
     5281 aggtgaacct atccctgcca ttcactatat gatcgcctgc accggcacta atcacattcc
     5341 ttgtgtacct tatgccccgt tcggcactca ccaattagcc gactatgtac gtgaaggcat
     5401 taaacacagc aaagcaattt tactcgctca ccacggctta atcaccgcag ataaaaccct
     5461 cgaaaaagcc ctcggcatcg cccacgaggt ggaagtgatc gcagaatggt atttgaaact
     5521 gctcgctaca ggaaaaccta tcccaacgct aaacaaagaa caaatggata tcgtactcgg
     5581 aaaatttaag tcctatggtt cttgggtgga aagcgaacaa tagcctgcca taaataacat
     5641 acaagcggtg atatttctta aaatttttac aaacggagtg cagtatgcaa aatgaagtaa
     5701 tactcacgat gaaaaacatc agtaaatcct ttgcgggggt caatgcgtta aaaggtgttg
     5761 agcttacgct gcgtaagggc gaagttcacg cattaatggg cgaaaacggt gcaggtaaat
     5821 ccaccttgat gaaatgcttg ctgggcgtat atcaagccga tgaaggtgaa atcatatata
     5881 aaggtcagcc tgttcgcttt gagtcggtat tagaggcaca aaaagcgggc attagtatga
     5941 ttttccaaga actgaacctc attccccatt taacggtggc agagaatatt ttcttcgccc
     6001 gtgagccact caaacacggt ttagtggata aagacaaaat ggtggaagaa tcggctaaat
     6061 tgctgaattt ctttgagatt gatgttgatc caaacgatga agtgagaatg ttgtcggtag
     6121 ccaaacaaca gatggtagaa attgccaaag ccctttcatt tgatgtggaa gtactgatta
     6181 tggacgagcc gacctccgct cttaccgaaa aagagatcga taaattattt gaattggtcg
     6241 atagactgcg tgccaaaggg gtgagcattg tctatatttc tcaccgaatg gaagaattaa
     6301 aacgtatctg taaccatatt acgattttcc gtgatggcac ctatgtatct aaccataaat
     6361 tcaatgaaat cacgatggat gagatcatta ccaaaatggt tgggcgttca ttggataacc
     6421 atttcccacc aaaaaccgct gtggtgacaa acgacattat cctctcgatt atgaatgctg
     6481 aacgtaaggg agtgtttgaa ccattgaact ttgacttacg caaaggagaa attctgggca
     6541 ttaccggctt ggttggagca aaacgtactg aactggctcg agccatcttc ggagcagacc
     6601 cacttgatag cggtgagatt tttgtgcacg agaacaaagt cagcattaaa gatccgagtg
     6661 atgcaattaa agccggtata gcctatttgt cggaagaccg taagctcaat ggtgttgcag
     6721 taagaatgtc gattcgtgag aatatcacga tggcatctat ggataaagtt gccaatcaaa
     6781 taggtgtgat ttcttacgat gaagaagaaa aagcctcaaa aactttcatc gacaaaatgg
     6841 aaatcaaaac cccaacgatt gagcagaaag ttcaaaactt aagtggtggt aaccaacaaa
     6901 aagtggtaat tggtaaatgg ttattccgtg aagcaaaagt gatgattttt gatgagccga
     6961 ctcggggaat tgatgtcgga gcaaaatatg ctatctatca actgctcgat gagcttgctg
     7021 ccaatggcgt gggtgttatc gtgatctctt ccgaattgcc tgaagtcttg ggagtttccg
     7081 accgcatcat tgtaatgcgt gaaggtagaa tgacaggtat gctcgagact aaaaaaacca
     7141 accaagaaga aattatgcat tatgcaactg gtgttaaaaa tatgtttgct cgtgaatatg
     7201 gggtggaaaa atgaatgaaa aacaaaaaga aatgttaaga aaaatggctg cattggctgg
     7261 cttagtacta ttaatcatct ttttcagcgt aaccaatgaa tacttcttta cctccaataa
     7321 cattatgaca gtagggctac aaacctctac cattgcgtta attggtattg gggcaacctg
     7381 tgtgatctta accggtggaa ttgatttaag taccggttca gtagttgcac tctccggtgt
     7441 ggcagctgca atgattgtaa atgccggtgt acccgtacca ctaggaatga ttttagggat
     7501 tttggtcggc ggtatttgtg gcttaatcaa cggcatttta gtcaccagaa tgaaattgcc
     7561 tccgtttatt gctaccctcg gtatgatgat ggttgcccgt ggtttagcat tgtatgttac
     7621 caatgccgca ccagtttccg gaatgcctga atcctttgcc aatctcggta acggagcatt
     7681 atttaaaatt gtggaagaag gggcaaacgg cttgccgaaa gtggtatttg caggtattcc
     7741 ttaccctgtg attattatga tctttattac cgtactattc actttcgctc tctctaaact
     7801 gaaagtcgga cgctatcttt acgcaattgg ttctaacgaa gaagccgcac gcttatccgg
     7861 cattaaaacc aatattgtca aaatctatgc ctatgttgcc agtggcttac tctctggttt
     7921 aactggcgtt atccttgcct cccgcctcgt caccgcccag ccaaacggtg gggtctctta
     7981 cgagctggat gcgattgcca gtgctgtggt gggcggcaca tcgcttatgg gcggtgtggg
     8041 tactatcccc ggcacactca tcgggtcatt cattatcggg gtactccgta atggtttgaa
     8101 tatgaatggc gtttcttcat ttgttcaaat gattgtcatc gggcttgtga ttatcgtggc
     8161 ggtctctctc gaccagctcc gccaatccaa aaaagccggt aaaaaataat ccactcttaa
     8221 aggagaatta aaatgaaaaa atcagcatta tttactgcga ccctctttgg tttatcgacc
     8281 ttatttgcag gcagtgcaat ggcaaaatct gatgaaatcg ctgtgattgt gaaatctgcc
     8341 aactctacct tctggcaaaa cgtgcgtaaa ggggctgaaa ctgccggcaa agatattgga
     8401 ggacaataca aagtcacctt ccaagggccg gaagccgaaa cagcgattga cgcccaagtg
     8461 aatatggtgg acaacgccgt aaaccgtggc gtggcaggca tcgtactggc tgcctcagac
     8521 ccggtagcat tagtgccggc ggtgaaaaaa gcttatgaga gtggcattcc ggtggtgctt
     8581 atcgattccg gcatcaacag tgacggcaaa tactaccaat ctttccttgc caccgacaac
     8641 cgtgcagcag gtaaattagc agcggaaaaa ctgcttgcca aagtcaaagg cggcaaagtg
     8701 gcagtgatga gctttaccca aggggcagca tcagcgattg agcgtaccgg tggttttatt
     8761 gacgaaatca aagcaaaagc cgattacaaa ctggtcggcc cgtactactc cgattcggat
     8821 atgaccaaag cgttaaacca aaccgaagac gtattaggtt ctaaccctga tcttgcagcg
     8881 atcttcgggg cgaacgaacc gactgcggta ggaatggcac gagcggtgaa acaaaaaggc
     8941 tttgccggca aaattgtagc ggtgggcttt gatggaaact ccgacctaca aaacttcgtg
     9001 cgtgacggaa cactagacgg catcgtggtg caatcttcct accaaatggg ctataaaggc
     9061 gtggacacca tcggcaagct gattaaaggc gagaaagtcg aaaaagtgat tgataccggg
     9121 gtggtttatg tcacgaaaga aaacattgat agcgaagaag cgaaaggtgt tctttactaa
     9181 cattcaagtt aaacggggtg gggttgccca ccctttcagc cgaaaggaag ccaatatgtc
     9241 aaataccctt accgataaag ccttagtggt gattgccgca cttggcggtc gagaaaatat
     9301 tgtcgatgtt tacgcctgta ttacccgcct acgtgtggag gtgaaaaata ccgacattgt
     9361 ggaatgggat aaaattaaat ctgtcggtgc gttaggcgtt gtgacctcag gctcagttat
     9421 tcaatctatt tttggtagcg atgccgaagc ctaccgtgat gaaatcaaac gtattttgaa
     9481 tatttagcct aagcacactc acaacaaaag aatcgcaaca agcggtgaaa tttttatcaa
     9541 aatttgcaaa aaaatgacgt aatttgaccg cttgcaagtt aatcgaattg tttaactttt
     9601 aagaaggaaa atagtatggc aaatcgtctt atcctcaatg aaaccagtta ccacggcaaa
     9661 ggagcaatcc aaaatattgt tcacgaagtg accagccgga gtttcaaaaa agcccttgtg
     9721 gtaacagaca aagacctgat caaatttaac gttgcccaaa aagtcaccgc cttgcttgat
     9781 gccgcaggct tgccttatga gattttcgat gaggtcaaag ccaacccggc agtcgatgtg
     9841 atcaaagcgg gggtcgcaaa attcaaagcc tccagtgccg attatttaat tgcgattggt
     9901 ggcggctcac cgattgatag tgccaaagcg attggtatta tcatcaataa ccctgagttt
     9961 agcgatgtac tttcattgga aggtgtcgcc ccaacccacc aaaaatgtgt gccgattatc
    10021 gccattccaa ctaccgcagg tacagccgca gaagtcacga ttaactatgt cattaccgat
    10081 gaagcgaaaa aacgcaaatt tgtgtgcgtg gacgttcacg atgtgccggc agtggcgtta
    10141 gtcgatccag atatgatgga atctatgccg aaaagcttgg ctgccgcaac cggtatggac
    10201 gctctcaccc acgccattga aggctacatt accaaagcgg cgtgggaatt aaccgatgct
    10261 ctacacctta aagcgattga gctgatcggc cgttcattgc gtggtgcagt ggagaaaaat
    10321 ccgaaagatg tcgaggcaat ggcattggga caatatgtgg ctggaatggg cttctccaac
    10381 gttgggcttg gcgcggttca cagtatggca cacccgcttt ctgcctatta cgacacccca
    10441 cacggcattg ccaacgcagt attactgccg tatgtgatgg aatttaataa aaattacacc
    10501 ggcgaaaaat accgtgaaat tgcccgagca atgggggtaa aaggcgtaga tgaaatgagc
    10561 caagaagagt accgcaacgc cgccattgct gccgtacaac agctttcaca agatgtcggc
    10621 attccgccaa aactgcatca aatcggcgtt aaagaagccg atttagcggc attatcggtc
    10681 gatgccttta atgatgtctg caccggtggc aacccgagag attgtacggc tgaggagctg
    10741 ttggaggtat atcggatcgc atactaagtt ctatctttgt taaatagaca agcggtgatt
    10801 ttttaataaa aatttgcaat ttctgttaaa aaaatactga tagtcataat gcccgatttc
    10861 tcatatttat ggaaaaatcg ggcattttcg ttgctttttt atcgccaaga aatgtgattt
    10921 tgttcacatt tatatctata aattaacaaa a
//
LOCUS       DANLYI010000067        10943 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.067, whole genome shotgun sequence.
ACCESSION   DANLYI010000067 DANLYI010000000
VERSION     DANLYI010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 10943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10943
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.067"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            complement(<1..1263)
                     /locus_tag="PXD81_001269"
     CDS             complement(<1..1263)
                     /locus_tag="PXD81_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HDL5537293.1"
                     /translation="MDSIPLSSLFITLAILLVLSAFFSGSETGLMSLNRYKMRHLAEQ
                     GHKGAKLAEKLLARTDVLLSLILICNNLVNIAASAIATMIGMQLSGEAGVAIATGLLT
                     FVMLVFSEILPKTVAAIYPEKVGFFASFVLTPLKKLLSPIVFLMNLIIAGLMKLLRIQ
                     QKDKQGLSSEELRSVVLDAGKFIPAEHREMLVSILDMEKVTVDDIMVPRNDISGIDID
                     DDWKAIMRQLNHAPHARVIIYKGDMDNNIVGILRVREAFRLMLEKNEFTKEMLLRATD
                     EVYFIPEGTPLTTQLMNFKANKERIGLVVDEYGDIKGLVTLEDILEEIVGEFTTSSAP
                     TLEQEVQQQSDGTVIIEGSANLRDLNKLFDWDLPLEEARTFNGLILEHLEKIPDEGME
                     FELYNLKVMILEVSENMVKQAKVEHLSPK"
     gene            complement(1387..2184)
                     /gene="ccsA"
                     /locus_tag="PXD81_001270"
     CDS             complement(1387..2184)
                     /gene="ccsA"
                     /locus_tag="PXD81_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HDL5537294.1"
                     /translation="MLLPLLSIVAYALALLWVAPTLVNLENAATVNKKPNMMLVFGFG
                     LLATILHFSVLYNELFLIEGQNFTVTNVISLMSFIMAACVTLALLKWKTVWFPLIIVY
                     SFAICSVGVAGFVKGSFIRNVQENAGLLFHLGIALFSYALFFLALIYAFQLKWLDRKL
                     KSKTMFFCQILPPLMTVERHFFTLTLAAQAMLTLTLFSGVVYLHNFFEPEHIHKAIFS
                     FLAWLVYSIQLLGQWKLHWRGKRVLIYSISGMMLLTIGYFGSRLIVN"
     gene            2400..2924
                     /gene="yceD"
                     /locus_tag="PXD81_001271"
     CDS             2400..2924
                     /gene="yceD"
                     /locus_tag="PXD81_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005621677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HDL5537295.1"
                     /translation="MQKVKLPLTIDPYKDAQRRVDYEGYISGKLLTRLGESVCNVQSD
                     AQVTLSLYVDPQRLTVIKGTAKVDVEFDCQRCGDPFTQTLDCSFVFSPVSNMDQADIL
                     PEIYEPIEVNEFGEVNLLDMIEDEFILELPLVPMHSEEHCEVSVSEQVFGELPEELVQ
                     KPNPFAVLANLKKN"
     gene            2941..3111
                     /gene="rpmF"
                     /locus_tag="PXD81_001272"
     CDS             2941..3111
                     /gene="rpmF"
                     /locus_tag="PXD81_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006231206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HDL5537296.1"
                     /translation="MAVQQNKKSRSRRDMRRSHDALTTAAVSVDKTSGETHFRHHVTA
                     DGYYRGRKVINK"
     gene            3130..4152
                     /gene="plsX"
                     /locus_tag="PXD81_001273"
     CDS             3130..4152
                     /gene="plsX"
                     /locus_tag="PXD81_001273"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HDL5537297.1"
                     /translation="MVDITLAVDVMGGDFGPRVTIPALEKALKQNPSLSFLLFGDEHQ
                     IAPLLQKHTLLNHPKIQIHHTEQVIEADVPFTTAIRQSKNSSMRLAIEAVKEGKAQGC
                     VSGGNTGALMGLAKLLIEPLPNIERPALTSLIPTLNGKSSVMLDLGANIEVSNHQLQQ
                     FAEMGNLFAQVMLDLVYPRLSLLNIGTEENKGTAQLQAVHQLLKNRQDLNYTGFIESD
                     KLTSYLTDVIICDGYTGNIALKALEGAAKNIISLFKKEKADSNICRNTKRYLLKAMFY
                     RYYRKLQQINPDRHNGATLLGLSKVVVKSHGGANINAFYYAIDYAVQQIQGDIPNKIL
                     EGLTQK"
     gene            complement(4249..5532)
                     /gene="hisD"
                     /locus_tag="PXD81_001274"
     CDS             complement(4249..5532)
                     /gene="hisD"
                     /locus_tag="PXD81_001274"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HDL5537298.1"
                     /translation="MQTLIWNNLTETEQQQYLARPAQVVGESIKAAVNEIKANVIANG
                     DKALFELAEKFDKVKLESLVVSEQQIQQAQSRISPELKQAIQNAKANIEAFHKAQQNQ
                     EVDIETQPGVCCQVITRPINAVGLYIPGGSAPLFSTVLMLAVPAKIAGCNKIVLCSPP
                     PIADEILYTAHLCGVETIYAVGGAQAIIAMANGTQTVAKVDKIFGPGNAFVTEAKRQV
                     VQEGTAIDMPAGPSEVLVIADEFADPAFVASDLLSQAEHGADSQVILVTPSESLARAV
                     DEELEKQVAVLPRAETARKALAHSRTFIAENLQQAVEISNKYAPEHLIVQTENARSLL
                     ADLDNAGSIFLGAYSPESMGDYASGTNHVLPTYGYTKTYSSLGLADFSKRMTVQELTP
                     QGFLALAETVERMASAELLDAHRNAVSLRVAKLKG"
     gene            complement(5544..6443)
                     /gene="hisG"
                     /locus_tag="PXD81_001275"
     CDS             complement(5544..6443)
                     /gene="hisG"
                     /locus_tag="PXD81_001275"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HDL5537299.1"
                     /translation="MTTQNRLRIAMQKKGRLSQECSELLKQCGVKIIWNDQRLIAYSE
                     NMPIEILRVRDDDIPGLVFDGVVDLGIIGENVLEEVELGRLSAGENVAYKKLRQLDFG
                     GCRLSLAIDRDKNYNNIKDLEDAVIATSYPNLLKRYMKEQNVPFKSILLTGSVEVAPS
                     AGIANAICDLVSSGATLEANGLKEVEVIYRSTACLIQRQEPLSAEKQALVDKLLTRIQ
                     GVQQANESKYIMLHAPKDKLAEITALLPGVENPTILPLANDQTRVAMHVVSQENLFWE
                     TMEQLKEMGASSVLVLPIEKMLA"
     misc_feature    complement(6476..6603)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            6802..7659
                     /locus_tag="PXD81_001276"
     CDS             6802..7659
                     /locus_tag="PXD81_001276"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HDL5537300.1"
                     /translation="MQISLKKIYSGKVRDLYEIDEKRMLMVATDRLSAFDVILDDPIP
                     RKGEILTQISNFWFNKLAHIMPNHFTGDSVFDVLPQEEAEAIQHRAVVCKRLIPVKIE
                     SIVRGYLTGSGLKDYQQTGTICGLTLPEGLVEASKLPQPIFTPSSKAEVGDHDINISY
                     EECERQIGQELAAKVRDAALKLYTEAANYALTKGIIICDTKFEFGLDENGELTLMDEV
                     LTPDSSRFWSVETYREGTNPPSFDKQFVRDWLENSGWNKQPPAPKIPAEVIEKTVAKY
                     QEALDLLTK"
     gene            complement(7800..9281)
                     /gene="ilvC"
                     /locus_tag="PXD81_001277"
     CDS             complement(7800..9281)
                     /gene="ilvC"
                     /locus_tag="PXD81_001277"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011199229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HDL5537301.1"
                     /translation="MANYFNTLNLRQKLDQLGRCRFMDRDEFADGCNFLKGKKIVIVG
                     CGAQGLNQGLNMRDSGLDIAYALRAEAIAEKRASFKRASENGFVVGTYEQLIPEADLV
                     INLTPDKQHSKVVADVMPLMKQGAAFGYSHGFNIVEEGEQIRPDLTVVMTAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGLAIAKAWASATGGDRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSIVCYDKLVSEGKDPAYAGKLIQYGWETITEALKQGGITLMMDR
                     LSNSAKLRAFELSEQIKEELAFLFEKHMDDIISGEFSSTMMADWANGDANLLKWREET
                     GKTAFENAPQADGIKISEQEYFDNGVLMVAMVKAGVELAFDTMVSAGIYEESAYYESL
                     HELPLIANTIARKRLYEMNVVISDTAEYGNYLFSHVATPILAEKIIPTLEKGDLGEPT
                     PAVEIDNVTLRDVNDAIRNHPIELIGQELRGYMTDMKRISSQG"
     gene            9503..10738
                     /locus_tag="PXD81_001278"
     CDS             9503..10738
                     /locus_tag="PXD81_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="paraquat-inducible protein A"
                     /protein_id="HDL5537302.1"
                     /translation="MKINRIILQSCSECGQQVRVPLTSNGNAVCPVCHNILRKSNTWS
                     LHRSAFLALAILILLPFALTFPLMSIDLLGIKINATVWDGIWKMATEGYPYTAFMVLV
                     CAVIMPIAFVLLILTIQLQRIIRQRPRYTLIFLTKIKEWVMLDVYLVALAVAAFKIRD
                     YADLQFDINLIAFVLVTLLNTLLFIKTEPKQAWERFYPEYHALPFDHPSNPTLCPTCE
                     YCFDETILDLKGRQRCPRCESNLSISDNVKIQRVWACLIAGTIMMIPANIFPISTTEL
                     AGQVSADSLMSGVLLFMEMGSYTVAAIVFIASIAVPVSKITIIFYLLLAIHYKWKHPI
                     HWQMKLLHYVHFVGRWSMLDLFVLSLMMSLLERGQILSFSVGEAAFYFGAAVFLTMFA
                     SANLDSRMLWKIHTVQKTS"
BASE COUNT         3258 a         2451 c         2070 g         3164 t
ORIGIN      
        1 tttcggtgaa agatgttcaa ctttcgcttg tttaaccata ttttcactca cttcaagaat
       61 catcactttc aagttgtaga gttcaaattc cattccttca tcaggaattt tttctaaatg
      121 ctctaaaatc aagccgttaa aggttctggc ttcctcaagc ggtaaatccc aatcaaatag
      181 tttgttcaga tcacgcaggt tggctgagcc ttcaataatc accgtaccat ctgattgttg
      241 ctgaacttct tgctcaaggg tcggtgctga tgaagtcgta aattcaccca caatttcttc
      301 taaaatatct tcaagagtga ctagcccttt aatatcacca tactcatcaa ccactaaacc
      361 aatacgctct ttattcgctt taaagttcat taattgtgta gtgagtggtg tgccttcagg
      421 aataaaatag acttcatctg ttgcacgcag tagcatttct ttggtaaatt cgtttttctc
      481 caacattaaa cgaaaggctt cacgcacccg caaaatccca acaatattgt tgtccatatc
      541 ccctttgtag ataatcaccc gagcgtgagg ggcgtgatta agctgacgca taatcgcttt
      601 ccagtcatca tcaatatcaa tgccgctgat gtcgttgcga ggcaccataa tgtcatcaac
      661 cgtcactttt tccatatcta aaatagagac caacatttca cggtgttcgg caggaataaa
      721 tttaccggca tcaagcacta cactacgcag ctcttcggag cttaagcctt gtttatcttt
      781 ttgttgaatc cgcaataatt tcatcagccc ggcaataatc aaattcatta aaaatacaat
      841 cgggctgagt aattttttaa gcggtgttag cacaaagctg gcgaaaaaac cgactttttc
      901 agggtaaatt gccgctactg ttttaggtag gatttccgaa aaaaccaaca ttacaaaagt
      961 tagcaaaccg gttgcaattg ccacaccggc ttcaccggat aactgcatac cgatcatcgt
     1021 tgcaatcgca gaggcggcaa tgttgactaa gttattacaa attaagataa ggctaagtag
     1081 cacatcggtg cgtgccaaga gtttttccgc cagtttcgcc cctttatgcc cttgctcggc
     1141 aaggtgacgc attttgtagc ggtttaaaga catcaagccg gtttccgagc ctgaaaaaaa
     1201 tgcagagagt actaaaagaa tagctaaggt aataaataaa ctactcaggg gaatactgtc
     1261 caaaattcaa ttcctatgta aattaatcat aaaaggtagc gtaacactaa aagcgttgtc
     1321 actacctcac tttgtcggtt cagccgactt gcaaaaaatt ttgctttttt gaccgcttgt
     1381 agatcattaa ttgacgatca aacgactacc gaaatagccg atggtgagca acatcatacc
     1441 cgaaatcgaa taaataagca cccgttttcc acgccaatgt aatttccact gtccaagtaa
     1501 ctgaatgcta taaacaagcc aagctaaaaa ggagaaaatt gctttatgaa tatgctccgg
     1561 ctcgaaaaag ttatgcaaat aaaccacacc ggaaaataaa gtgagcgtca gcatagcttg
     1621 tgcagctaag gttaaagtaa agaaatggcg ttcaaccgtc attagcggtg gtagaatctg
     1681 gcaaaagaac attgttttgc tctttagttt tctatccagc catttgagtt gaaaagcata
     1741 aattaatgct aaaaagaaga gagcataaga aaataatgca atccctaaat ggaaaagcag
     1801 tcccgcattt tcttgcacat tacggataaa actacctttt acaaatccag ccacccccac
     1861 actacaaatg gcgaaagagt acacaataat taaaggaaac caaaccgttt tccattttaa
     1921 caacgcaagt gtgacacaag ccgccataat gaaactcatc aaagagatta catttgttac
     1981 cgtaaaattc tgcccttcga ttaaaaataa ttcattatat aacacagaaa aatgcaaaat
     2041 cgtggcaagt aacccaaacc caaagactaa catcatatta ggctttttat taactgttgc
     2101 ggcattttct aaattcacta gagtaggggc aacccaaagt agcgctagag cgtaagcaac
     2161 aatggataaa agaggaagta acatattgtc tcgaatctcg tcaaataaga ataactatca
     2221 gaattttaat caaaagtaac cgttttagct agtttttgct gttgctagat caaaaattat
     2281 cctaaaaaat gtgcttttcg ccttaaattg aaccctacct tttctcggta agattatctc
     2341 actgaaaaca ataattttcc tttgactaat ttgctttcta tctgtaatat gcacacccta
     2401 tgcaaaaggt aaaactaccc cttacgattg acccttacaa agatgctcag cgtcgcgttg
     2461 attatgaagg ctacatttca ggtaaacttc taacacgctt gggtgaatca gtatgtaatg
     2521 tgcaaagtga tgcacaagtc actctctcgt tatatgtcga tccacaacgc ctaactgtga
     2581 ttaaaggcac agcaaaagta gatgtggaat ttgattgcca acgatgtggt gacccgttta
     2641 cacaaacgct cgactgttca tttgttttca gtccggtgtc taacatggat caggcggaca
     2701 tcttgcccga aatttatgaa cctatcgaag taaacgagtt cggtgaagta aatttactag
     2761 atatgattga agatgaattt attcttgaat tacctctagt cccgatgcat agtgaagaac
     2821 actgtgaagt gtccgtaagt gaacaggtat ttggcgagtt gccggaagaa ctggtacaaa
     2881 aaccaaaccc gttcgcagta ttagctaatt taaagaaaaa ctagatctag gagaaagccc
     2941 gtggctgttc aacaaaataa gaaatctcgt tcacgtcgtg atatgcgtcg ttcacacgat
     3001 gcattaacaa ctgcagcagt ttcagtagat aaaaccagtg gtgaaactca ctttcgccac
     3061 cacgtaactg cagacggtta ctaccgtggt cgtaaagtaa tcaacaagta attcctttcg
     3121 aggtttatct tggttgatat cactcttgca gtagatgtga tgggcgggga ctttggtccc
     3181 cgcgttacta ttcctgcact ggaaaaagcc ttaaaacaaa atccaagcct ttctttttta
     3241 ttatttggtg atgaacacca aatagcccct ttattacaaa aacacacact actcaaccac
     3301 cccaaaatcc aaattcatca taccgaacaa gtgattgaag ctgatgttcc gttcactact
     3361 gcaatccgcc aaagcaaaaa cagctctatg cggttagcga ttgaggcagt aaaagagggc
     3421 aaagcacaag gttgtgttag tggcggtaac actggtgcgt taatgggatt ggcaaaatta
     3481 ttgattgaac cattaccaaa tattgaacgc cctgcactca cttcgctaat tcctaccctc
     3541 aacggcaaat ccagcgtaat gttagattta ggggcaaata ttgaagtaag caatcaccag
     3601 ctacaacaat ttgcagaaat gggaaacctg tttgctcaag taatgcttga tttagtttac
     3661 cctcgcctgt ctttattgaa tattggtaca gaagaaaata aaggcacggc acaacttcag
     3721 gcagtgcatc aactgctgaa aaacagacaa gacttaaatt acaccggctt tattgaaagc
     3781 gataaattaa ccagctatct cactgatgtg attatttgtg acggctacac cggcaatatc
     3841 gcgttaaaag cgttggaagg ggcagctaaa aatattattt ctctctttaa aaaagagaaa
     3901 gccgattcca atatttgccg taacaccaaa cgatatttac tgaaagcgat gttctaccgt
     3961 tactaccgca aattgcaaca aattaaccct gaccgccata acggtgcgac cttgctcggc
     4021 ttatctaaag tagtcgtgaa aagccacggc ggggcaaata tcaatgcctt ttattacgct
     4081 attgattatg cggtgcaaca aattcaaggc gacattccaa ataaaatcct agagggttta
     4141 acccaaaaat aagcgaactc aactgttcgc ttattttcat ttcttgtttc acttaaagat
     4201 acaagcggtc aaatttgcaa aaaaatttac ccattcaacc gcttgtgttt agccttttaa
     4261 ttttgccact cgtaaactaa ccgcattgcg gtgtgcatct agcaattctg cacttgccat
     4321 tcgttccacc gtttcggcca aggctagaaa gccttgtggt gtcagctcct gcaccgtcat
     4381 tcgcttgcta aaatcagcta aacctaagct cgaataggtt ttagtgtagc cgtaagtcgg
     4441 taaaacgtgg ttagtgccgc tagcataatc ccccatactt tccggcgaat acgcccctaa
     4501 gaaaatcgag ccggcattat ctaaatccgc caacaacgag cgagcatttt cagtttgcac
     4561 aattaaatgc tcgggtgcat atttattact aatttcgacc gcttgttgca ggttttccgc
     4621 aataaaagtg cgactgtgag ccaatgcttt acgggcagtt tcggcacgag gtaaaaccgc
     4681 cacctgcttt tctaattctt catcgactgc ccttgccaag ctctcacttg gtgtgactaa
     4741 aatcacttgg gaatcggctc catgttctgc ttgagagagt aaatcgctcg ccacaaatgc
     4801 agggtcagca aactcatcgg caatcaccaa cacttccgac gggcctgccg gcatatcaat
     4861 cgccgtgcct tcttgcacaa cttggcgttt cgcctccgtc acaaacgcat tgcccgggcc
     4921 gaaaatttta tccactttcg ctaccgtttg tgtgccattt gccatcgcaa taatcgcttg
     4981 agccccccca actgcgtaaa tcgtttccac cccacacaaa tgggcggtgt agagaatttc
     5041 atcggcaatc ggcggtggtg agcaaagcac aatcttgtta caaccggcaa tttttgccgg
     5101 cactgcaagc attaacacgg tagagaaaag cggggctgaa ccgcccggaa tataaagccc
     5161 caccgcatta atcgggcgag tgatgacttg gcaacacacc cccggctggg tttcaatatc
     5221 cacctcttga ttttgctgtg ccttgtggaa agcctcgata ttcgctttcg cattttgaat
     5281 cgcttgctta agctctggtg aaatacggct ctgtgcttgc tgaatctgct gttctgatac
     5341 aactaagctc tctaatttta ctttatcgaa cttttcagcc aattcaaata atgccttatc
     5401 gccgttggca attacattcg ctttaatttc attaactgcc gctttaatac tctcgccaac
     5461 cacctgtgcc ggacgggcaa gatattgctg ctgctcagtt tctgttaaat tgttccaaat
     5521 tagggtttgc atatttattc cttttacgcc aacattttct caatcggtaa aaccaatacg
     5581 gaacttgcgc ccatttcttt tagctgctcc attgtttccc agaacaggtt ttcttggctg
     5641 acaacgtgca ttgccacacg ggtttggtcg ttagctaacg gtaaaatagt cgggttttcc
     5701 acaccgggta ataaagcggt aatttccgcc agtttgtctt tgggggcgtg cagcataata
     5761 tacttagatt cgttcgcctg ttgcacacct tgaatgcggg ttaataattt atccactaac
     5821 gcttgttttt ccgctgaaag tggttcttgg cgttgaatta ggcaagcagt tgaacggtaa
     5881 atcacttcaa cctcttttaa tccgttcgcc tctaatgttg cacctgaaga aaccaagtca
     5941 caaatcgcat tcgcaattcc ggcacttgga gcaacttcaa ccgaaccggt gagtaaaata
     6001 cttttaaacg gcacattttg ctctttcata taccgcttta ataaattggg gtaagaagtg
     6061 gcaatcacag catcttctaa atctttgata ttgttataat ttttatcacg atcaatagct
     6121 aacgataaac ggcagccacc aaaatctaac tgacgaagtt ttttataggc aacattctca
     6181 ccagcactta atcgccctaa ttccacttcc tctaatacat tttcaccgat aatcccaaga
     6241 tcaactacgc cgtcaaaaac taagcctgga atgtcgtcat cacgcacccg taaaatttca
     6301 atcggcatat tttctgaata ggcaatcagg cgttgatcgt tccaaatgat tttcacgcca
     6361 cattgtttaa gcaactcact acattcttgg cttaagcgac cttttttctg catcgcaatt
     6421 cttaatcgat tttgtgttgt cattgttgtt tccttcttat gttttaaatt gtaaaaataa
     6481 aaaaacctct cgaaatcact tccgagaggg ttaaatttat gctgcgttta ggcgtactcg
     6541 gaagtcttat cttccgcaca taccaaatgc ccgaaagatt attcaggtaa atgatggtgg
     6601 tgatgagtac gaataaaagc cattttgttg ttctccaaac taaattgctt taaaagatac
     6661 gcttaaattt tggcaatgac aagtgttttt tgcgaaaaaa ttgctatttt tcatctctct
     6721 aaaacaacta tcgcaaacgt ttgcgtaaag cggtataatt tgcctgattt tttgcaaatt
     6781 caacaaagta ggaataacac tatgcaaatc agcctcaaaa agatctactc cggcaaagtc
     6841 cgtgatctct acgaaatcga tgaaaaacgt atgcttatgg ttgccaccga ccgattatct
     6901 gcctttgatg ttattttaga cgatcctatt cctcgtaaag gcgaaatcct cacccaaatc
     6961 tccaacttct ggttcaacaa attagcccac attatgccaa accacttcac cggcgacagt
     7021 gtgtttgacg tgttaccgca agaagaggca gaagctattc aacatcgtgc ggtggtttgc
     7081 aaacgcttaa taccggtgaa aattgaatcg attgtgcggg gttatttaac cggctccggg
     7141 ctgaaagatt accaacaaac cggcacgatc tgcggcttaa cattgccgga aggcttggtt
     7201 gaggccagca aattaccgca accgatcttc acaccgtcca gcaaagccga ggtgggcgat
     7261 cacgatatta atatcagcta cgaagagtgt gaacgccaga tcggtcaaga acttgccgcc
     7321 aaagtgcgtg atgccgctct aaaactctac actgaagcgg caaactatgc cctgaccaaa
     7381 ggaattatta tttgcgacac caaattcgaa ttcggcttag acgaaaacgg tgaactaacg
     7441 ctaatggacg aagtcctaac cccggattcc agccgttttt ggtcagttga aacctaccga
     7501 gaaggcacaa atccaccttc atttgataaa caattcgtgc gtgactggct ggaaaatagc
     7561 ggttggaaca aacaaccccc agccccgaaa ataccggcag aggtaattga aaaaaccgta
     7621 gcgaaatatc aagaagcatt ggatttatta accaaataac accacaagcg gtcaaattta
     7681 cgctattttt tgcaaaacaa aaacctgtaa gccaacactt acaggttttt tagttagtgg
     7741 taaaaagtgc ggttattcta ccgcactttt gcaaaatttt gtgtaaatct gaccgcttgt
     7801 tagccttgcg aagaaatacg tttcatatct gtcatataac cacgtaattc ttgaccgatt
     7861 aactcgattg ggtggttacg gattgcgtca ttcacatcac gtaaggtaac gttgtcgatt
     7921 tccactgccg gagttggttc acctaaatcg cctttctcta acgttgggat aattttctcc
     7981 gctaaaatcg gggttgcaac gtgagagaat aagtagttac cgtattctgc ggtatctgaa
     8041 attaccacgt tcatttcata taaacgctta cgagcgattg tatttgcgat taacggtaac
     8101 tcgtgtagag actcgtagta agccgattct tcataaatac cggctgaaac cattgtatca
     8161 aaggctaatt caacccctgc tttcaccatt gcaaccatta acacaccgtt atcaaagtat
     8221 tcttgctcag aaattttaat accgtctgct tgtggtgcgt tttcaaaggc tgttttacct
     8281 gtttcttcac gccattttaa taagttggca tcgccgtttg cccagtctgc catcatcgtt
     8341 gatgagaact caccactgat gatgtcgtcc atatgttttt caaataagaa cgctaattct
     8401 tctttgattt gctcagaaag ctcgaacgca cgcaatttgg cactgtttga taagcgatcc
     8461 atcattaagg tgataccgcc ttgttttaac gcttcggtaa tggtttccca accgtattgg
     8521 atcaatttac ctgcgtaagc cggatcttta ccttccgaca ctaatttgtc gtagcacacg
     8581 attgaacctg cttgtaacat accgcaaaga atggtttgtt cacccattaa atcagatttc
     8641 acttctgcta cgaatgaaga ttctaatacg cccgcacggt caccaccggt agcacttgcc
     8701 cacgctttcg caatggctaa accttcgcct ttagggtcat tttccgggtg aacggcaatt
     8761 aaggttggca caccgaaacc acgtttgtac tcttcacgca cttccgtgcc cgggcatttc
     8821 ggagcggtca tcacaaccgt taaatccgga cgaatttgct caccttcttc cacaatgttg
     8881 aaaccgtgtg aataaccaaa tgccgcacct tgtttcatta acggcattac atcggcaact
     8941 actttagagt gctgtttgtc cggcgttaag ttaatgacta aatccgcttc aggaattaat
     9001 tgctcgtaag tacccaccac aaagccgttt tcagaagcac gtttaaatga agcacgtttt
     9061 tcagcaatcg cttcggcacg taaagcgtaa gcaatatcta agcctgaatc acgcatattt
     9121 aaaccttggt ttaaaccttg agcaccacaa cccacaatca cgattttttt gccttttaag
     9181 aaattgcagc catctgcaaa ttcatcacga tccataaagc ggcaacgacc taactggtct
     9241 aatttttgac gtaaatttaa agtgttgaaa tagttagcca ttttttaatc cttatttgaa
     9301 ttaatgagat tgatatgatg ttttgtttgc acattgttat agtttgattt gattatacct
     9361 taacctaaca ttgcgtaaat tgaaaatatt aatatttcat attgcataaa atgcaacttg
     9421 caaaaatttt gccaaattta accacttgca atctctacga taaagtagaa aataaccacc
     9481 taatttaata gaagatcaaa aaatgaaaat aaacagaatc attttacaaa gctgtagcga
     9541 atgtgggcaa caagtgcgag tcccgctcac gtcaaatggc aatgccgttt gcccggtctg
     9601 ccataatata ttacgcaaaa gcaatacttg gagcttgcac cgtagtgctt ttttggcatt
     9661 agccatttta attttgcttc ccttcgcact tacttttccc ttgatgagca ttgacttact
     9721 cggcatcaaa attaatgcta ccgtatggga cggtatttgg aagatggcaa cggagggcta
     9781 cccttacacc gcttttatgg tgctagtctg cgcggtaata atgccgattg catttgtatt
     9841 gctaatttta accattcagc tacaacgtat tattcgccaa cgcccccgtt acacgctgat
     9901 tttcttaact aaaataaaag aatgggtaat gcttgatgtc tatttagttg ctcttgcggt
     9961 tgccgctttt aaaattcgag attatgccga tctacaattc gatatcaact taatcgcctt
    10021 tgtgttggta acactcttaa acaccctact ttttattaaa accgagccaa aacaagcatg
    10081 ggaacgtttt tacccagaat atcacgcctt gccgtttgat cacccaagca atccaacact
    10141 ttgcccgacc tgtgaatatt gctttgatga aactatttta gacttaaaag gacggcaacg
    10201 ctgccctcgt tgtgaaagca atttatctat ttccgacaac gtaaaaatac aacgggtttg
    10261 ggcttgctta attgcaggga ctattatgat gattccggca aatattttcc cgatttcgac
    10321 aactgaactg gcaggtcagg tttcggcaga ttcattaatg tccggcgtgc ttctgtttat
    10381 ggaaatgggt agctacaccg tggcagcgat tgtgtttatc gctagtattg cggtgccggt
    10441 gagcaaaatt accattattt tttatctctt gctggcaatt cattacaagt ggaaacaccc
    10501 gattcattgg caaatgaaac tactacacta cgtccatttt gtcggacgct ggtcaatgct
    10561 ggatttattc gtgttatcat tgatgatgtc gctactcgaa cgagggcaga ttctcagttt
    10621 ctcggtaggg gaagccgctt tctattttgg agcagccgta ttcttaacga tgtttgcctc
    10681 tgcaaattta gattccagaa tgttgtggaa gattcatact gtccagaaaa catcctaaat
    10741 aaagattttc cgataaataa tacataacat gcaaagtaag aaaagtagta cgtaacccaa
    10801 aatctcaatc cctattgagt aagaaagagc cgataatgta gcaccgatag ccagaaagaa
    10861 attagcaaat ataaaatagt ctttactata tttacttgag gcaattaggg tatccattgc
    10921 agtactaaag aacatttcca aat
//
LOCUS       DANLYI010000068        10867 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.068, whole genome shotgun sequence.
ACCESSION   DANLYI010000068 DANLYI010000000
VERSION     DANLYI010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 10867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10867
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.068"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            25..957
                     /locus_tag="PXD81_001279"
     CDS             25..957
                     /locus_tag="PXD81_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL5537303.1"
                     /translation="MDSLIHFLSAFLTNITWIGALELGLIYSLVALGVLISYKILDFP
                     DLTADGSFPLGGAVCVISILNQVDPWLATLYGTLAGAVAGMVTASLHIGFKIEKLLAS
                     ILMMIALYSINLRIMGMPNIAILGETTIYDVMPIESDLQLALVRLVMALLVVIIVKGL
                     FDLFFATEIGLAVRATGTNSRMAKAQGIAINKMTILGMAISNALIALGGALYVQSNGG
                     VDISIGVGTIVIGLAAVIIGEAIFSVKRIIWLTFAVIIGSILYRFFIALALNNETLNG
                     IGFGPQDLNLITALLVVIVLAVPRIKHKLLGKRG"
     gene            959..1756
                     /locus_tag="PXD81_001280"
     CDS             959..1756
                     /locus_tag="PXD81_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015432965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL5537304.1"
                     /translation="MIELNNLFITFNKGTAIENPVLRGLSLKVEQGEFVSVIGSNGAG
                     KSTLLNAISGDCAVDSGEILIQGNKVNQSTTWQRANQVARVFQDPMAGTCESLTIEEN
                     MALAYQRGRKRGLGFAIKRQMRELFKDKLALLGLGLENRLTDQMGRLSGGQRQAVSLL
                     MASLQPSNILLLDEHTAALDPKTTDFVLELTNKIVREQKLTTLMVTHSMRQALDYGDR
                     TVMLHQGQVAFDVSGEQRQKMDVPDLLQLFQQSRHEQLSDDGLLLGN"
     gene            1843..2064
                     /locus_tag="PXD81_001281"
     CDS             1843..2064
                     /locus_tag="PXD81_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5537305.1"
                     /translation="MLSIFKTPVEKETLDDWAKISVDVAKVAILAIPVILFGKEPTLI
                     KLTNLALLVLSIYVFLTLGRKLRQLKEVL"
     gene            2247..4172
                     /locus_tag="PXD81_001282"
     CDS             2247..4172
                     /locus_tag="PXD81_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HDL5537306.1"
                     /translation="MTKYDDKIQHAFSENGLLSQNISGFRPRQAQLEMAQVVAKAVKF
                     ATPVVVEAGTGTGKTFAYLVPALLSGKKTILSTGSKNLQDQLFNRDLPTIQKALKYKG
                     KIALLKGRANYLCLERLDQVTAMGVLGDKSVLTDLKKVSKWQNATKTGDLSECITIAE
                     DSPILPQLVSTTESCLGSDCPHFKDCYVVQARRKAMEADVVVVNHHLFCADMAVKKTG
                     FGELIPEAELVIFDEAHQLPDIASQYFGQSLSSRQLFDICKDTNIVYRTELKDLSQLG
                     KAADHLTKVVQDFRLLMGGEGQIRGNLRELFRDKKVVDGLVKVAENIDFLSEVAKKSL
                     GRSETLDKIFERLAEVKVQLKKLMDTSVTGYCYWYEANGRSFGLHITPLMVADKFGEQ
                     LKNQQIGWVFTSATLEVGGKFDHFCQRLGIENAEQLVLQSPFDYQHQSLLCVPRYLPD
                     TNKSNTLTALGQLLKPVIEANKGRCFLLCTSYYMMRGLADFLREHSSLNVLLQGETSK
                     SRLLEKFVKEDHSVLVATQSFWEGIDVRGDDLSLVIIDKLPFTSPDEPLLKARMEDCK
                     IQGGDPFNDIQIPEAVITLKQGVGRLIRDVTDRGVVIICDSRLVMRNYGQTFLKSLPP
                     SRRTRDLGNVIQFLKQK"
     gene            4246..4611
                     /locus_tag="PXD81_001283"
     CDS             4246..4611
                     /locus_tag="PXD81_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HDL5537307.1"
                     /translation="MLSTLLRMVASLALLFVMLYLGKLIVYLFPIGIPDSILGMLILF
                     GCLVIGIVKVEWVIPSAKPLTRYITLFFLPICVELIEHLELLRQNLNTFVLATALSTL
                     VSVVAIGVFAQWIFHYKRG"
     gene            4613..5299
                     /locus_tag="PXD81_001284"
     CDS             4613..5299
                     /locus_tag="PXD81_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005597218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HDL5537308.1"
                     /translation="MSYLYTVLTVIMFIAGLKISQKLKSSFLNPFLIALALLIGVLLI
                     FNIPYQDYYQGNSPLNNLLGVSVVALAYPFYEQLPHIRKKWRQIVVILLFSTIITMIT
                     GLGFSLLFGASQEILAAILPKSVSMPMAIIITNEINGNVAIAAVGVMIAGLVGSILGF
                     TLLRLAKVRNVMVIGLSMGAASHALGTARCLEHSMKAGSYSSIALVACGVLSSIFAPL
                     IFNVIVTLCY"
     gene            5383..7146
                     /gene="msbA"
                     /locus_tag="PXD81_001285"
     CDS             5383..7146
                     /gene="msbA"
                     /locus_tag="PXD81_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HDL5537309.1"
                     /translation="MQEQITQEKDLSTLKTFKRLWPTISPFKAGLITGAVALVLNALV
                     DSSLIYLLKPLLDEGFGQANNDFLKLMAVLVVVFILVRGITNYIASYCLAWVSGKVVM
                     TLRRHIFQHLMYMPVSYFDNNPTGRLLSRVTYDTEMVASSASHSLVTIVREGAYLLSL
                     FVVMIYTSWQLSIVLFVMAPIIGVLISFVSKYFRQLSRNIQSSMGELTVTTEQMLKGH
                     KVVLSFGGQEIEKARFDRVSNDMRRKGMKVVSADGISDGVVQLIASFALSAVLYVATF
                     PEVMSENLTAGSFTVVFSSMMAMLRPLKSLTNVNSQFQRGMAACQTLFEFLDLKTEKD
                     HGLISVAKAEGNVEFKKVSFSYEGKEEKALDNISFTIPKGKTVALVGRSGSGKSTIAN
                     LLTRFYDVTDGQILLDGINIQDYRLKNLREQCSVVSQQVHLFNDTIANNIAYAAIDKY
                     SREEIIAAAKAAHAMEFIEKLENGLDTVIGENGASLSGGQRQRLAIARALLRNSPVLV
                     LDEATSALDTESERAIQSALEVLQKDRTVLVIAHRLSTIEKADEILVVDHGNIIERGS
                     HTELLAQGGAYKQLHSLQFGA"
     gene            7235..7867
                     /gene="lolB"
                     /locus_tag="PXD81_001286"
     CDS             7235..7867
                     /gene="lolB"
                     /locus_tag="PXD81_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005597216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein localization protein LolB"
                     /protein_id="HDL5537310.1"
                     /translation="MKKLTKLLSLSALLVLTACNSVLNASTEVQQPTVQIPHDDPQWQ
                     QHLAKLSQIQGYTATGQFGYISPEERFSSHFNWRYTNPIQFGLELSSNLSTKSLKLER
                     NKRGLTVSDNEGNSRSDRDVDALMQEIIGVSFPIDQFAYWVKGLPEKDGNYIVNEKRQ
                     LSQFSYPLNGTLWKASYVEYHEDRVPNLPKLIVLENGTQTLKIRIEKWAY"
     gene            7855..8709
                     /gene="ispE"
                     /locus_tag="PXD81_001287"
     CDS             7855..8709
                     /gene="ispE"
                     /locus_tag="PXD81_001287"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HDL5537311.1"
                     /translation="MGVLMKKVTLPSPAKLNLFLYITNKRADGYHELQTLFQFLDFGD
                     EIEIEITESSEVELINPVEGVATEENLIYRAAKLLQKTTACRQGCRIGIKKNLPMGGG
                     VGGGSSNAATVLVGLNHLWNTELSLEKLAELGLSLGADVPIFVRGMAAFAEGVGEVLT
                     PCDPQEKWYVVLKPEVSISTAAVFTDPNLPRNTPKRPLAELLSLPWENDCEKVVREKY
                     SAVEDLIQELLQYAQFRLTGTGACIFAEFETKAEAEQVFAHKPEAVFGFVAKGQNISP
                     LHQMLFQI"
     gene            8735..9685
                     /locus_tag="PXD81_001288"
     CDS             8735..9685
                     /locus_tag="PXD81_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HDL5537312.1"
                     /translation="MTDIKLFAGNATPELANRIAKHLYTSLGNATVGRFSDGEIQVQI
                     NENVRGGDIFIVQSTCAPTNDNLMELIVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVIADFLSSVGVDRVLTCDLHAEQIQGFFDVPVDNVFGSPVLIDDILKKS
                     DLVNPIVVSPDIGGVVRARAVAKLLNDTDMAIIDKRRPKANVSQVMHIIGDVADRDCI
                     LVDDMIDTGGTLVKAAEALKERGARRVFAYATHAVFSGTAAQNLANPALDEIVVTDTI
                     PLSNEIKALNKVRVLTLSGMLAEAIRRISNEESISAMFDA"
     gene            9763..10773
                     /gene="pyrD"
                     /locus_tag="PXD81_001289"
     CDS             9763..10773
                     /gene="pyrD"
                     /locus_tag="PXD81_001289"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HDL5537313.1"
                     /translation="MYSLIRKLLFALDTEKAHQFSIQALALAGKSPFSILPTPDNPTQ
                     VMGLTFKNPIGLAAGADKNGEAIDGFGKLGFGFIEVGTITPLAQDGNPKPRQFRIVDA
                     EGIINRNGFNNKGVDVLVENVKNATYSGILGINIGKNAVTPIEHSFDDYQICLRKVYQ
                     YADYVAVNISSPNTKNLRSLQYGEALDDLLRNLKNEQAQLSQKMGKYRPLVLKIAPDL
                     TDEEIVSVADSLIRYQIDGVIAGNTTLSRESIAGLPYSEQLGGLSGKPLHSLSTRLIS
                     KLSAELGERIPIIGCGGVHSLESGQEKVNAGASLLQVYSAMIYQGPSLIQTLAKHLQL
                     NS"
BASE COUNT         3114 a         1961 c         2506 g         3286 t
ORIGIN      
        1 gatttcatta actataataa acaaatggac agtcttattc attttttgag tgccttttta
       61 actaatatca cttggattgg ggcgttagag ctggggttaa tttattccct tgttgccctt
      121 ggggtattaa tttcttataa aattctggat tttccggatc tgaccgcaga cggcagtttc
      181 cctttaggag gggcggtgtg tgtgatcagc attttgaatc aagttgatcc ttggctggca
      241 actttatacg gcacgcttgc cggagcagta gccggtatgg ttactgcaag tttgcatatc
      301 ggctttaaaa tcgaaaaatt gcttgccagt attttaatga tgatcgcact ctattcgatt
      361 aatctacgca ttatgggaat gccgaatatt gctattttag gcgaaaccac gatttacgat
      421 gtaatgccga ttgaaagcga tctacagtta gccttagtac gattagtgat ggcattgctg
      481 gttgttatta tcgtaaaagg tttgtttgac ctcttttttg cgaccgaaat cggtttggct
      541 gtgcgggcaa ccggtactaa tagtcgaatg gcgaaagcac agggtattgc gattaataaa
      601 atgacgattt tagggatggc gatttctaat gcgttaattg ccttaggtgg tgctttgtat
      661 gtgcaaagca acggtggtgt agatatttcg attggggtag gaactatcgt gattggcttg
      721 gcggcggtaa ttatcgggga agcaattttt tcggtaaaac ggattatttg gcttaccttt
      781 gcggtgatta ttggctcaat tctttatcgt ttcttcatcg cactagcatt aaataacgaa
      841 actttaaacg gcattggctt tggcccgcaa gatctgaatt taattacggc gttattggtg
      901 gtgattgtat tagcggttcc tcgaattaaa cacaaattgc tcggtaagcg aggttaatat
      961 gattgaactc aataacttat ttattacctt caacaaaggg acagcaattg agaaccctgt
     1021 gctgagaggc ttatccctaa aagttgagca aggcgaattt gtgtcggtaa ttggtagcaa
     1081 tggggcgggt aaatctacct tattaaatgc gatcagtggt gattgtgcgg tggactccgg
     1141 tgagatttta attcaaggaa ataaggtaaa ccaatccacc acttggcaac gggcaaatca
     1201 agtcgcccga gttttccaag acccgatggc tggcacttgt gaaagtttaa cgattgaaga
     1261 aaatatggca ctggcttacc aacgtggacg taaacgtggg ttgggctttg cgatcaaacg
     1321 ccaaatgcga gagttattca aagacaaact tgctttgtta gggctaggtt tggaaaatcg
     1381 gcttactgat caaatggggc gtttatcagg cgggcaacgc caagcggtga gcttgctgat
     1441 ggcatcactt cagccgtcta atatcttatt gttagatgaa cacaccgcgg cactggatcc
     1501 gaaaaccacc gattttgtgc ttgaacttac caataaaatt gtaagagaac agaaattaac
     1561 cacgctaatg gtaacgcatt caatgcggca agcccttgac tatggcgata gaaccgtgat
     1621 gttacaccaa gggcaagtgg cgtttgatgt ttcgggcgag caacggcaaa aaatggacgt
     1681 gccggatctg ttacaacttt tccaacaaag tcgccacgag cagttgagtg acgatggatt
     1741 attactcggc aattaatttt gcgatctgca tcgaaaaaat gaatttctcc ttttgttttt
     1801 ggctgtcaaa ttttaaataa taagcccaaa aggaggtatt caatgttaag cattttcaaa
     1861 acaccggtag agaaagaaac gctagacgat tgggctaaaa tttcggttga tgtagcgaaa
     1921 gttgcgattt tggcaattcc tgttatactt tttggcaaag aaccaacgtt gattaaattg
     1981 actaacttag cattattggt attgagtatt tatgtttttt tgacgcttgg gcgaaaatta
     2041 cggcagctaa aggaggtgtt gtgatggaac taggctggaa tttaaccata ggtttgctaa
     2101 ttttaaacgt ctttgcgttt gcttggcttt atattattaa ccggatttct aaagaacaat
     2161 tatccaaaaa tcaataatat ttaatttttc acaagcggtt gtttttgcaa aatattttgt
     2221 aaattcgacc gcttgcctgt ttttaaatga caaaatacga cgataaaatt cagcatgcat
     2281 ttagcgaaaa tgggttgctc agccaaaata tcagtggatt tcgaccacgc caagcacagc
     2341 ttgaaatggc tcaagtggtc gcaaaagcag taaaatttgc gacacctgtt gtggtagagg
     2401 cgggaaccgg cacaggcaaa acctttgctt atcttgtgcc ggcactactt tcaggcaaga
     2461 aaactattct ttcgacaggt tctaaaaacc tgcaagatca actttttaat cgagatcttc
     2521 ccactattca aaaagccctg aaatacaaag gcaaaattgc gttactcaaa gggcgtgcga
     2581 actatttgtg tttagagcgt ttagatcaag tgaccgcaat gggcgtactt ggggataaat
     2641 ccgttcttac cgatttaaaa aaagtcagta aatggcaaaa tgccactaaa acaggggatt
     2701 taagtgaatg tattaccatt gccgaagata gcccgattct accgcagtta gttagcacca
     2761 ccgagagttg cttaggctcg gactgcccgc attttaaaga ttgttatgtg gtacaagccc
     2821 gccgtaaagc gatggaggca gacgtagtgg tggtaaacca tcacctattc tgtgctgata
     2881 tggcagtaaa aaagaccggt tttggtgagt tgatccctga agctgagctg gtgatctttg
     2941 atgaagctca ccaactgccg gatattgcca gccaatattt tgggcaatcg ctttcttccc
     3001 gccagttatt tgatatttgt aaggatacca atattgttta ccgcactgaa ctcaaagatc
     3061 tgtctcaatt aggcaaagcg gctgatcact tgacgaaagt agtgcaagat ttccgattat
     3121 tgatgggcgg agaagggcag attcgaggca atttgcgaga gttatttcgt gataaaaaag
     3181 tggtcgatgg cttggtaaaa gtggctgaaa atattgattt tttaagtgaa gtggcgaaaa
     3241 aatcgctggg gcggtcggaa acgttggata aaattttcga gcggttagca gaagttaagg
     3301 ttcaacttaa aaaattaatg gatactagtg tgacaggcta ttgttactgg tatgaggcga
     3361 atggacgatc ctttgggctg catattacgc cgcttatggt ggcagataaa tttggtgaac
     3421 agttgaagaa ccagcaaatt ggttgggtgt ttacctcagc cacactcgaa gttgggggca
     3481 aattcgatca tttttgtcaa cggctgggca tcgaaaatgc ggagcagttg gtgttacaaa
     3541 gtccgtttga ttaccaacat caatctttac tctgtgtgcc ccgctacttg ccggatacca
     3601 ataaatccaa tactttaact gctctcgggc aattattaaa accggtgatt gaggcgaata
     3661 aagggcgttg tttcttgctt tgtacttcct attatatgat gcgaggttta gccgactttt
     3721 tacgcgaaca cagttcgctg aatgtgttat tgcaaggcga aaccagcaaa agccgtttat
     3781 tggaaaaatt tgtgaaagaa gatcacagcg tgttggtggc gacccaaagt ttttgggagg
     3841 ggattgatgt gcgaggcgat gatttatcgt tggtgattat tgataaattg ccgttcactt
     3901 caccggacga accattactc aaagcccgaa tggaagattg taagatacag ggcggtgatc
     3961 cgtttaacga tattcaaatt cccgaagcgg tgattaccct caaacaaggt gtagggcgtt
     4021 taattcgtga tgtgaccgat agaggtgtag tgattatttg tgattcccgt ttggtgatgc
     4081 gaaattatgg gcaaactttc ttgaaaagtt taccgccgtc tagacgcact agagatttgg
     4141 ggaatgttat tcaatttttg aaacaaaaat agggttacaa gcggtcagat ttctgcagat
     4201 ttttgcaaaa aaacggataa aaataaccgc ttgttgagga gttatatgtt gtctacattg
     4261 ctgcgtatgg ttgcttcttt ggcattgtta tttgtgatgc tttatttagg caagttaatc
     4321 gtttatctat tccccatcgg gatcccggat agtattttag gtatgcttat cttattcggc
     4381 tgcttggtta tcggcattgt taaagtggaa tgggtgatac cgagcgcgaa accgctcaca
     4441 cgttatataa cactattttt ccttccgatt tgtgtcgaac tcatcgaaca tcttgagctg
     4501 ttacgccaaa atctgaacac ttttgtatta gcaacggcat taagcacctt agtttctgtg
     4561 gtcgcgattg gcgtgtttgc acagtggatt tttcattaca agagagggta aaatgagcta
     4621 tctttacact gtgttgacgg taatcatgtt tattgccggt ttgaaaatca gccaaaaact
     4681 caagagcagt tttctgaacc ctttcttaat tgcactcgct ttgctaattg gtgtattact
     4741 gatttttaat atcccttacc aagactatta tcaaggtaat tcccccttga ataacttact
     4801 tggcgtttct gttgtggcac ttgcttaccc tttttatgaa caattacccc atatccgtaa
     4861 aaaatggcga cagattgtcg tgattttgct gtttagtacc atcattacga tgataaccgg
     4921 gcttgggttt tcattattat ttggggcaag ccaagagatc ttagccgcca ttttgcctaa
     4981 atcggtatca atgccaatgg caattattat tacgaatgaa attaatggaa atgttgcaat
     5041 cgcagcggtt ggggtgatga tcgcaggctt agtgggatct attttaggct ttacgcttct
     5101 gcgcctggca aaagtaagaa atgtgatggt gattgggtta agtatgggag ctgcttccca
     5161 cgcattaggc acggcaagat gcttagagca tagtatgaaa gccggtagct atagttctat
     5221 cgcattagtg gcttgtggtg tgctgtcttc tatatttgca cctttaatat ttaacgttat
     5281 cgtcacgtta tgttactaaa aacagtgaaa tctcaaacct tatttggtaa ctggctgaaa
     5341 tcaaggtaca ataccgtgat ttttcaatga aaatagagaa ttatgcaaga acaaataaca
     5401 caagaaaaag atctttctac gcttaaaacc tttaagcgtt tatggccgac tatcagcccg
     5461 tttaaagcgg gtctgattac aggtgctgtc gctttagtat taaatgcgtt agtggattct
     5521 agtcttatct atctcctcaa acccttgcta gatgaaggct ttgggcaagc taataatgat
     5581 tttctgaaat tgatggcggt gcttgtagta gtttttattt tagtgcgcgg tattaccaat
     5641 tatattgcca gttactgttt agcttgggtg tcaggcaagg tggtaatgac attacgccgc
     5701 catatttttc aacatttaat gtatatgccg gtaagctatt ttgataacaa cccgaccggg
     5761 cgtttgctct cacgagtgac ttacgatacc gaaatggtgg cgagttctgc ttctcattct
     5821 ttagtgacta ttgtgcgtga aggggcttat ctgctgtcat tgtttgtggt gatgatctat
     5881 accagttggc agctttctat tgtgttattt gtgatggctc ctatcattgg ggtactgatc
     5941 agtttcgttt caaaatattt tcgtcaatta agtcgcaata tccaaagttc aatgggggag
     6001 cttactgtca cgacagaaca aatgcttaaa gggcataagg ttgtactttc ctttggtggg
     6061 caggaaattg agaaagcacg ttttgatcga gtcagtaatg atatgcgtcg taaagggatg
     6121 aaagtcgttt ctgctgatgg catttctgat ggtgtggtac agcttatcgc ctcttttgcg
     6181 ttatctgctg tgctttatgt tgcgaccttc cccgaggtga tgagtgagaa tttaaccgca
     6241 ggttcattca cagtagtgtt ttcttcgatg atggcaatgt tacgtcctct aaaatcactc
     6301 accaatgtga actcgcaatt ccaacgtgga atggcagcct gccaaacgtt atttgaattt
     6361 ttagatttaa aaactgaaaa agatcacggc ttaatctccg ttgctaaagc tgaaggcaat
     6421 gtggaattta aaaaggtaag ctttagttat gaaggtaaag aggaaaaagc actcgataat
     6481 atttctttca ccattcctaa aggcaaaaca gtagcattgg ttgggcgttc cggctcgggt
     6541 aaatcaacta ttgccaattt gcttacccgt ttttatgatg tgactgacgg tcaaattttg
     6601 cttgatggaa ttaatattca agattaccgc ttaaaaaact taagagaaca atgctcggtg
     6661 gtttctcaac aagttcattt gtttaacgat actattgcta ataatattgc ttatgctgca
     6721 atcgacaaat acagccgaga agaaattatt gctgctgcaa aagctgcaca cgcaatggaa
     6781 tttatcgaaa aattggaaaa tggcttagac actgtaattg gtgaaaatgg cgcaagtctt
     6841 tccggtgggc aacgccaacg cttggcaatc gctcgtgcat tactgcgaaa ttctcctgtg
     6901 cttgtgctgg atgaagctac ctctgcatta gatacggagt cagaaagagc gatccaatct
     6961 gcacttgaag tgctacagaa agatcgtacg gtgttggtca tagcacaccg gttatcaacg
     7021 attgaaaaag ccgatgaaat tttggtagtc gatcacggaa atattattga gcgtggcagc
     7081 catacggaat tgttagcaca aggtggggct tataaacaac tccatagttt acaattcgga
     7141 gcctaatttg taggggagaa atatgtttct ccccaattaa tgataaaaac agggcgaaaa
     7201 atatttcgtc cctacaataa tttatttgga tactatgaaa aaactgacaa aacttttatc
     7261 tcttagtgct ttactggtct taaccgcctg taattcggta ctaaatgcct caacagaggt
     7321 gcaacaacca acagtacaaa ttccgcatga cgatccacaa tggcagcagc atttagcgaa
     7381 actgagccaa attcaaggct atactgcaac ggggcaattt ggctatattt cgcctgaaga
     7441 gcgtttttct tcccatttca actggcgtta tactaatcca atccaatttg ggttagagct
     7501 ttcctcgaat ctctcgacta aatcactgaa attagagcgt aataaacgag gcttaacggt
     7561 ttcggacaat gagggtaata gccgttctga ccgtgatgtt gatgcgttaa tgcaagagat
     7621 tatcggtgtt tcgtttccta tcgatcagtt tgcctattgg gtaaaaggct tgcctgaaaa
     7681 agatggcaac tatattgtga atgaaaaacg ccagctgtcg caatttagtt atccgcttaa
     7741 tggcacgctt tggaaagcaa gctatgtgga gtatcacgaa gatcgtgtac caaacttacc
     7801 gaagctgatt gtgcttgaaa acggcactca aacgttgaag atccgcattg aaaaatgggc
     7861 gtattaatga agaaagtcac cttacccagc ccggcaaaat taaacctgtt tttatacatt
     7921 accaataagc gggcagacgg ttatcacgag ctacaaacgc tgtttcagtt tttagatttt
     7981 ggtgatgaaa ttgagattga gatcacagaa agttcagagg ttgaattaat taatccggta
     8041 gaaggggtgg caactgagga gaatctcatt taccgagcag caaaactttt gcaaaaaacg
     8101 accgcttgta ggcaaggctg ccgtattggg attaaaaaaa acctaccgat gggcggaggt
     8161 gttggtggtg gatcttctaa tgctgcaaca gtattagtcg gtttaaatca cttgtggaat
     8221 accgagcttt cattggaaaa attggcagaa ttaggcttaa gtttaggggc tgatgtgccg
     8281 atatttgtta gaggtatggc tgcttttgcc gaaggcgtgg gggaggtgtt aactccttgt
     8341 gatccacaag aaaaatggta tgtagtgcta aaaccagaag tgtcgatttc aaccgcagcg
     8401 gtgtttaccg acccgaattt acctagaaat acgccaaaac gccccttagc cgaattatta
     8461 tctttaccgt gggaaaacga ttgcgaaaaa gttgtacgag aaaagtattc tgcggttgaa
     8521 gatcttatac aagaattgtt acaatatgct cagtttaggc taacaggcac aggtgcttgt
     8581 attttcgccg aatttgaaac aaaagcagaa gccgagcagg tttttgcaca taaaccggaa
     8641 gccgtcttcg gttttgtggc gaaagggcaa aatatttcgc cattacatca aatgttattc
     8701 caaatctaac catcttaacc ttaaggatca atccatgaca gatattaaac tgtttgcggg
     8761 caacgcaact ccggaacttg ctaatcgtat tgcaaaacat ctttatacct cattaggtaa
     8821 tgcaacagtt ggtcgtttta gcgatggtga aattcaagta caaatcaatg aaaatgtgcg
     8881 tggaggcgat atttttattg tgcaatccac ttgtgcacca actaatgata atttaatgga
     8941 attgatcgtg atggtagatg cgcttcgccg tgcatcagct ggtcgtatta ccgcagtgat
     9001 tccgtatttc ggttatgcac gccaagacag acgcgtgcgt tcggcccgtg tgccaattac
     9061 cgcaaaagtg attgctgatt tcttatccag cgtaggggta gatagagtct taacctgcga
     9121 cttacacgca gagcaaattc aaggtttctt tgatgtgccg gtagataacg tatttggttc
     9181 ccctgtgtta atcgatgata ttttgaaaaa atccgatttg gtcaacccta tcgtggtttc
     9241 tccagatatt ggtggggttg ttcgtgcaag agcggtggca aaattattaa atgataccga
     9301 tatggcaatt attgataaac gtcgtccaaa agcgaatgta tcacaggtaa tgcacattat
     9361 cggtgatgtg gctgatcgtg actgtatttt agttgatgat atgatcgata ctggtggtac
     9421 tttagtcaaa gcggctgaag cgctgaaaga acgtggcgcc cgtcgtgtct ttgcttatgc
     9481 cacacacgcg gtattttcag gcacagcagc tcaaaattta gcgaatcctg ctttagatga
     9541 aattgtagta actgatacta ttccactgag taatgagatt aaagcattaa ataaagtacg
     9601 tgttttaaca ttatccggta tgttagcaga agcaattcgc cgtattagca atgaagaatc
     9661 catttcagca atgtttgatg cctaatttat gagaaaaaac ccagtcgaaa gattgggttt
     9721 ttcatatctg ttcactacta tatttagttt aagataattt gtatgtattc cctgattcgt
     9781 aaattacttt ttgcactaga tacggaaaaa gcacatcaat tctctattca ggcacttgct
     9841 ttagccggaa aatcgccttt ttcgattctg ccaacacccg ataatccgac ccaagtaatg
     9901 ggactcacgt ttaaaaatcc aatagggctt gcagccggag cggataaaaa cggtgaagcg
     9961 atagatggtt ttgggaaatt aggatttggg tttattgagg ttgggactat tactcctctc
    10021 gctcaagacg gaaaccctaa accgaggcaa tttcgtattg tagatgcaga gggaattatt
    10081 aaccgcaatg gatttaataa taaaggcgtt gatgttttag tagaaaatgt aaaaaatgca
    10141 acctatagtg gtattttagg tattaatatt ggaaaaaatg cggttacacc aattgagcat
    10201 agctttgatg actaccaaat ttgtttgcgt aaggtttacc aatatgctga ttatgtggcg
    10261 gtgaatattt catcacccaa taccaaaaat ttgcgcagtt tacaatatgg tgaggcatta
    10321 gatgatttgc tgcgaaacct caaaaatgag caggcacaac tttcgcaaaa aatggggaaa
    10381 tatcgaccgc ttgttttaaa gattgcgcct gatttaaccg atgaagaaat cgtttctgta
    10441 gccgatagtc ttattcgtta ccaaattgat ggggtgattg ccggcaatac taccttgtct
    10501 cgtgaaagta ttgccggatt accgtattcg gaacaactgg gtggattaag tggcaagcca
    10561 ttacactcat taagtacaag gttaatttca aaattatctg ccgagttggg cgagagaata
    10621 ccaattatag gttgtggggg agtccattcg ttagagagtg ggcaggagaa agtgaatgca
    10681 ggtgctagtt tattgcaagt ctattccgca atgatttacc aaggaccatc attaattcaa
    10741 accttagcca aacaccttca gctgaacagt tagggataga tttctagtta aaaatgattg
    10801 gttaggttta tttctgtcat cttagtggaa tagatggcgg tattagctat ctatcaaaaa
    10861 tagacaa
//
LOCUS       DANLYI010000069        10788 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.069, whole genome shotgun sequence.
ACCESSION   DANLYI010000069 DANLYI010000000
VERSION     DANLYI010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 10788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10788
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.069"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            1..2385
                     /locus_tag="PXD81_001290"
                     /pseudo
     CDS             1..2385
                     /locus_tag="PXD81_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005715618.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent
                     hemoglobin/transferrin/lactoferrin family receptor"
     gene            2440..2946
                     /gene="hutX"
                     /locus_tag="PXD81_001291"
     CDS             2440..2946
                     /gene="hutX"
                     /locus_tag="PXD81_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme utilization cystosolic carrier protein
                     HutX"
                     /protein_id="HDL5537314.1"
                     /translation="MISIEQQTALRNELAENPGQILEMLAAKYQCTLEEVMLNLPAEM
                     LKVTEGERFAEVLQQIHEWDDAITLISHTEDTIIEFVGKLPSGSISRGFYNFEHKEGG
                     GLQGHLRYENCAKIYLLDRPFMGKRTVSLIFINKKGNAMFKIFVGREKIGGALKEHQI
                     KALYQLIG"
     gene            complement(3026..4243)
                     /locus_tag="PXD81_001292"
     CDS             complement(3026..4243)
                     /locus_tag="PXD81_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018650987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter"
                     /protein_id="HDL5537315.1"
                     /translation="MKNKILGSALMIAGTTIGAGMLAMPLTSAGMGFGATALLLVGLW
                     ALLAYTGLLFMEVYQTAPQKDVGVASLAEQYFGLIGRVLATFSLLVLLYALLAAYITG
                     GGSLLAGVMPEMADADMKLKISILIFTAVLGTFVVVGVKSVDGLTRVLFLGKIVAFIA
                     VLLMMLPKAKLENLTAIPLDNLLIISAIPIFFTAFGFHVIMGTINSYLDADIGKIRKS
                     IYIGTAIPLVAYLLWQLATHGVLSQNEFTTILTQDPTLNGLVKATSQITGSTILGEMV
                     RLFSVLALITSFLGVSMGIFEGVGDLLKRLNLPTNRLWLTIATFTPPILFALFYPNGF
                     IKALGYAGLLFAFYGMILPIGLAWKARRQYPNLPYRVIGGNLALVVALIAGIIIMVIP
                     FLIQAGYLPQAVG"
     gene            4404..7937
                     /gene="mfd"
                     /locus_tag="PXD81_001293"
     CDS             4404..7937
                     /gene="mfd"
                     /locus_tag="PXD81_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HDL5537316.1"
                     /translation="MSLISLNLPKSDGNFQDHQTLGNLVGHSDTLAISQAARAFYGLT
                     VVVTPDTRTALRLEKALPQFAGLPVQLFPDWETLPYDNFSPHQDIISARLSALFELQQ
                     GRQQILLLPINTLMQKVCPPNYLANNVLLIKKGDRFSIQKLRLQLENAGYRAVEQVLE
                     YGEYAVRGSILDLFPMGAEEPFRLDFFDDEIDSIRTFDVDNQRTKTEIAEINLLPAHE
                     FPTDSNGIEHFRAKFREKFGEIRREPEHIYQQVSKGILNAGIEYWQPLFFEEMASLFD
                     YFPAQTLFITFADIQQKAEQFHQDTQNRYESHRVDPMRPLLPPSDLWFAIDEVNRWLK
                     GYPRLTITSEKIRKSAAKMNANVANLPDIAIQSSAKEPFSAFQQFRQKFDGNILFSVE
                     SEGRRETLLDLLAPLGVKPKQVNSLDEAQGLPFSLIISPLDQGFVVETGGHGTRPYDD
                     ELNTNGENDNNYVGAGPVPARNLAIICETDLLGERVQTRREKNRKAVNPDTLIRNLAE
                     LKIGQAVVHLENGVGRYGGLTVLDAGGIKAEYLVLHYANDAKLYVPVASLHLISRYIG
                     GADETAPLHKLGSEAWAKTRQKAAEKIRDVAAELLDVYAKRESQTGFAFAYDKAEFDQ
                     FSATFPYEETDDQKIAINAVISDMCQAKAMDRLVCGDVGFGKTEVAIRATFLAVMNHK
                     QVVILAPTTLLAQQHYENFKDRFANYPVNVEVLSRFKTAKEQKTILQNVAEGKVDILV
                     GTHKLLQDDVAFRDLGLLVIDEEHRFGVRQKEKIKQLRANVDILTLTATPIPRTLNMA
                     LNGMRDLSIIASPPARRLLIKTFVRQSDEMVIREAILREILRGGQVYYLHNDVATIEN
                     CAEKLAELVPEARIVIGHGQMRERELERVMSDFYHQRFNLLVCSTIIETGIDVPTANT
                     IIIERADKFGLAQLHQLRGRVGRSHHQAYAYMLTPHPKTLTKDAEQRLEAMSTIDNLG
                     AGFALATHDLEIRGAGELLGSEQSGQIESIGFSLYMDLLENAVKALQEGREPSLDEIT
                     QHQAEIELRVPALLPDDYLPDVNMRLSFYKRIASSENLQSLNDLKVELIDRFGLLPEA
                     TKNLFAITQLRLSAQTLGIKKIDAGIHGGYLEFKPTAQPDPMKFIALIQKQPAVYSFE
                     GAVKFKFRLALEDNRKRLEFVENLLREVVGG"
     gene            8100..8387
                     /locus_tag="PXD81_001294"
     CDS             8100..8387
                     /locus_tag="PXD81_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HDL5537317.1"
                     /translation="MEEINGFEWDSEKALRNQQKHGVSFIEAVSVFYDNNAMLIPDPD
                     HSSREERFLLLGRSNKDNLLVVVHCERNTNIRIISARLATKRESGQYKGVH"
     gene            8388..8600
                     /locus_tag="PXD81_001295"
     CDS             8388..8600
                     /locus_tag="PXD81_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="HDL5537318.1"
                     /translation="MRDEYDFSNAVKNPYAKGLKSKSVVTIRIEDDVISYFKQLAEEV
                     NLPYQTLINLYLKDCVEQKRKPTISW"
     gene            8716..8976
                     /locus_tag="PXD81_001296"
     CDS             8716..8976
                     /locus_tag="PXD81_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HDL5537319.1"
                     /translation="MEKIARLFRNGRNQAVRLPVEFEFDAEQVYVSKKENGDILLSLR
                     SRNTEHWQRLFELLPAVVCDETFLDTKERNQSFEQRDPFEVA"
     gene            8977..9366
                     /locus_tag="PXD81_001297"
     CDS             8977..9366
                     /locus_tag="PXD81_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HDL5537320.1"
                     /translation="MYMLDTNTVSYFFRGNANVVAKLKSINPERLCISSVTAAELVYG
                     VAKRNNGQLSQFLDYFLSSVKVLDWNYRCAELYGKLRAEMEKNGKVMGVQDQMIASHA
                     LAEECILVSSDQAFKMVPDLKLENWLH"
     gene            9386..10624
                     /gene="pepT"
                     /locus_tag="PXD81_001298"
     CDS             9386..10624
                     /gene="pepT"
                     /locus_tag="PXD81_001298"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HDL5537321.1"
                     /translation="MKQSLLNRLLRYTQIHTTSDPKSQTVPSSQQQWDLLHLLKTEVE
                     AMGLSEIYLDPNGYLFASLPANIDKTLPVLGLIAHVDTSPDFNGENVKPQVIEAYQGG
                     NILLNGSGDYLSPTDFPNLNKLIGDTLVTTDGTSLLGADDKSGIAIILSAIEYLQANP
                     HIPHGKIRIGFTTDEEIGRGADHFDVNAFGADVAFTFDGGPLGELQYENFNADAATVT
                     FFGRSVHPGTAKGKMINALERACEFQHRLPPHLTPATSEGREGFIMLHGIEGGIDKAE
                     IHYILRSFERDELAAFGKMLNDTLAEMQQIYGEHCGKVEIVEQYRNMREVLDDYGYLV
                     EIAENAMREQGIEPNLEPIRGGTDGSRLSFMGLPCPNLFTGGENFHGRFEFISVDTME
                     KATKVVIDICRKFADYDFNK"
BASE COUNT         3236 a         2155 c         2469 g         2928 t
ORIGIN      
        1 gactatattg gctttaagcg ttagtagcat tttgtcttca caagctacct atgcggaaga
       61 aacaacagcg gaattatctg aagtaaatgt tgttgctaaa gaagatgtga aggcttctat
      121 agaaaagaaa aataaagaga taattcagca agagttgatt caaaataatc gagatttagt
      181 tcgttatagc cctgatgttg gcattgttaa ccaaggtaga catcaaaaag gatttgctat
      241 tcgtggagtg gaagacaacc gtgtaggaat aagtattgat ggtgtctcgc ttttcctgat
      301 tcagaagaaa attcccttta taaacgctat ggtaatttaa atacatctcg ccaatctata
      361 gatccggaat tagctcgtac tattgaagta tctaaaggtg cagattcttt caatcaaggt
      421 agtggtaatt taggcggcgg agttaattat cggacgcttg agccttatga tattgttcgt
      481 aacggtaata aatttggtgc tttttatcgc acaggatatg gtactcgtaa taatgaatgg
      541 ttgaatacat tcggtgttgg atatttaggt gagaaagctg aggcgatatt ggtttattca
      601 aatcgccatg ggcatgaaat gaaaagtgca ggaggttata cacttcctga agattctcta
      661 caaactcgct ctttacgcag tagtaaacaa actcctgatg attctactca taatcatcat
      721 aattatttag ccaaatttgc ttatcgattt aatgataatc accgcgttgg tgtttcttac
      781 agtgggcaaa ataataaaaa ttatattatc gaagattcag ccgttactat tacttctttt
      841 tggcgtgaag cggaagaccg tagtaaacgt gatacagtaa atgtttttta tgagtatttc
      901 ccagagtcaa aatggatatc tctagcgaag atagatgcag attatcaaga tactgaaaca
      961 tctgcttata actatgaagg tacacgcgct gaagatgcaa cagactggag ccctgctaaa
     1021 caaagaaaac caagtgacaa taatattcgt atttttaata ctgaattgaa acgcttaaat
     1081 ttccgtttag acagccaagc attagaattt ggtaaatgga ctcatcagct atctttcaaa
     1141 gcctctactg cagagagaga tttcgatgta ttacataagg actcggttta tttatccggt
     1201 gtttgggtaa attctcccga ttctacgatg atgtacccaa ttaaaacgaa acagcacact
     1261 ttttcattgc acgataagat tgatttaacg catgattgga ttgcaacgct aggcattcgt
     1321 tatgattggg cgaagtatga acaacaagaa ctcaatggtc ttgtttgtcg taactgcgta
     1381 aaaactgaca atgcgaaatt taatcaacta acttgggtta ccggtttaga aaaacatctg
     1441 actgaggcat ggaaagtagg atataacatt gggacaggct tccgtattcc aaatgcatca
     1501 gaaatgtatt ttgattaccg tgataatgct gcaggagctt ggatgtccaa tccaaaccta
     1561 aaagcggaac gtagtttaag ccaaaattta agtttgcaag gtagtggcaa tgttgggctg
     1621 ctctcagtta atttccacca tactaaatat aaagattttt tatgggagca agaaacctgg
     1681 gatgtttacc aagcatatgg tagagagttt tggcgcccgg tacagcaaat gcaaaatatt
     1741 gattctgcga aaatctacgg gctagaagtg agtggtaaat ggaatttaaa ttcagctatg
     1801 ccaatacctg aaggctggaa gctatttgga gccttcggtt acagcaaagg tagtatgtct
     1861 aacggggcag atttactttc tattcagccg attaaagccg tagttggctt agattatgaa
     1921 caacctgaag gtaaatgggg agtattttct cgcttcacat tcttgggggc taaaaaggcg
     1981 aaagatgcaa aatatttaaa aacattaccg gaaaaatgcc ttaaagagga tagaacgcca
     2041 aatccgtatt acccttattg gggggatgaa tatgacattc gttgtactga atattctcac
     2101 gagacaggtt tagatacctg gaagcactta aatgctaaag cctttgttgt ggatttatac
     2161 ggtttctata aaccaactga gaatattaca ttacgtgccg gtatttataa tttgtttaac
     2221 cgtaaatacc atacttggga tactttacgt ggtttaaata caacaggtgg aatcgttaat
     2281 tctgttggag tacgtgaaaa tgcaacttac ggtggctatc ctggattaca acgctattat
     2341 gaacctggac gtaacttctc ggctaatttt gaataccgtt tctaatttac aaatttactt
     2401 agagagctaa gaaactctct ttacttaaag gaacatttaa tgatatcaat cgaacaacaa
     2461 accgccttac gcaatgaatt agccgaaaat ccggggcaaa ttttagaaat gttagcggca
     2521 aaatatcaat gcacgctgga agaggtaatg ctgaatttac cggctgaaat gctaaaagta
     2581 accgaagggg aacgttttgc cgaagtgcta cagcaaatcc acgaatggga cgatgcgatt
     2641 acgctgattt cgcatacgga agataccatt attgagtttg tgggaaagct cccaagcggt
     2701 tcaatttctc gtggatttta caatttcgaa cataaagagg gcggaggctt acaagggcat
     2761 ctacgctacg aaaattgtgc caaaatttat ttgttggatc gtccttttat gggaaaacgt
     2821 actgtttcac tgattttcat taataaaaaa ggcaacgcaa tgtttaagat ttttgtcggc
     2881 agagagaaaa ttggcggggc attaaaagag catcaaatta aggcgctgta tcagttgatt
     2941 ggctaatctg taaaaagtca tcgaaaaatg accgcttgta acttatagaa agggctacga
     3001 tattgaatcg tagccctttt caatattagc ctaccgcttg cggtaaatag ccagcctgaa
     3061 ttaaaaacgg aatgaccata atgataatac cggcaattaa cgcaaccact aatgccaaat
     3121 taccaccaat cacacggtaa ggtaaattcg gatattggcg acgtgctttc cacgctaagc
     3181 cgataggtaa aatcatacca tagaacgcaa agagtaaacc ggcgtaacct aatgccttaa
     3241 taaagccgtt cggatagaat aaagcgaaca gaattggcgg tgtaaaggtg gcgatggtca
     3301 gccataaacg gttagtcggt agatttaagc gttttaacaa atcgcccacg ccttcaaaaa
     3361 ttcccatcga aacccctaaa aatgaggtaa tcaatgcaag cactgagaat aagcgaacca
     3421 tttcgcctaa aatcgtacta ccggtaatct ggctagtggc tttcaccaag ccgtttaagg
     3481 tcggatcttg tgtcaaaatt gtggtgaatt cattttggct taataccccg tgtgtagcga
     3541 gctgccacaa taaataagcc actagcggaa ttgccgtccc gatataaata gatttacgaa
     3601 ttttgccgat atctgcgtct aaatagctat taattgttcc cataataacg tggaaaccga
     3661 acgcagtgaa gaaaatgggt atagcagaaa taatgagtaa gttatctaat ggaattgctg
     3721 tcaagttttc cagcttcgct ttaggcagca tcatcagcaa cacggcaata aaagcaacaa
     3781 tttttcccaa aaacagtact cgggttaaac catcaaccga tttcacgccg actaccacaa
     3841 atgtacctaa taccgcagtg aagattaaaa tgctgatttt cagtttcata tcagcatctg
     3901 ccatttcagg cattacaccg gctaacagtg aaccaccacc ggtaatataa gccgcaagta
     3961 gggcataaag tagtacgagc aaactgaaag tggctaatac acgcccgatt aagccaaagt
     4021 attgctcggc aaggcttgcc actccaacat ctttttgcgg agcggtttga taaacttcca
     4081 taaataataa accggtgtag gccaataaag cccataatcc gactaagagt aatgcggttg
     4141 ccccaaaacc cattcctgca gaggtgagtg gcattgcaag cattcctgca ccaatggtag
     4201 tgcctgcaat cattaatgca cttcctaaaa ttttattttt cataatatgt gtgagtaaga
     4261 gttaaaaaag gggaacattt tatcagaatt ttttcgcaat gcgatctaga taacataatt
     4321 tctccgctta ttttgcacaa tcagccgtgc tttagtaaac taagcaacaa tttgcaattt
     4381 tttatttaaa acagaccgct tgtatgtctt taatttcact taatctgccg aaatctgacg
     4441 gcaattttca agatcatcaa accctcggta accttgtcgg gcattccgat acccttgcga
     4501 tcagccaagc tgcccgagcc ttttacggct tgacggtggt tgtcacaccc gacacccgca
     4561 cggcgttacg cttggaaaaa gccttgcctc agtttgcagg gctgccagtg caactgttcc
     4621 ccgattggga aaccttgcct tatgacaatt tttcgccgca ccaagacatt atctccgccc
     4681 gactttctgc cctgtttgaa ctgcaacaag ggcgacagca gattttgctg ttgccgatca
     4741 acaccttaat gcagaaggtc tgcccgccga attatctcgc taacaatgtg ctgttgatta
     4801 aaaaaggcga ccgtttttcg attcaaaaac tgcggttaca actggaaaat gccggctatc
     4861 gggcggtgga acaggtgttg gaatatggtg aatacgccgt gcgtggctcg attttagacc
     4921 tattcccgat gggagcagaa gagccgtttc gcctcgattt tttcgatgat gagatcgaca
     4981 gtatccgcac ttttgatgtg gataaccaac gcaccaaaac ggaaattgcc gaaattaatt
     5041 tactgccggc acacgagttc ccgactgata gcaacgggat cgagcatttt agagccaaat
     5101 tccgtgaaaa attcggtgaa atccgccgtg agccggagca tatttatcag caagtcagca
     5161 aaggcatttt aaatgcaggg attgagtatt ggcagccgtt gttttttgag gaaatggcaa
     5221 gtctgtttga ctatttccct gcccaaacgc tgtttatcac tttcgccgat attcagcaaa
     5281 aagctgaaca atttcaccaa gatacccaaa accgctatga aagccaccga gtggatccga
     5341 tgcgaccgct actgccgccg agtgatctct ggtttgcgat tgatgaggta aatcgctggc
     5401 tgaaaggcta tccccgttta acgataactt cggagaaaat ccgtaaatca gcagcgaaaa
     5461 tgaatgcaaa tgtggctaac ttgccggata ttgctattca atcctctgcc aaagagccgt
     5521 tttctgcttt ccagcaattc cgccaaaaat ttgacggcaa cattctgttt tcggtggaga
     5581 gcgaagggcg aagagaaacc ttgctggatt tgcttgctcc gcttggggtg aagccgaagc
     5641 aggtcaattc attagatgaa gcacaaggtt tgccattttc cctgattatt tcaccgcttg
     5701 atcaagggtt tgtggtggaa acgggcgggc acgggacccg tccctacgat gatgaattaa
     5761 atacaaatgg cgaaaatgat aataactacg taggggcggg tcctgtgccc gcccgcaacc
     5821 ttgccatcat ctgcgaaacc gacctgctgg gcgaacgtgt gcaaacacgg cgtgagaaaa
     5881 accgcaaagc cgtcaatcct gataccttaa tccgtaattt agcagaactc aaaatcggac
     5941 aggcggtggt gcatttggaa aacggcgtgg ggcgttacgg tggcttaacc gtgctggacg
     6001 ctggtgggat caaagccgaa tatctcgtgc tacattatgc taacgatgcc aaactctatg
     6061 tgccggttgc ctcgctccat ttaatcagcc gttatatcgg tggggcggat gaaactgcgc
     6121 cgctacataa attaggcagt gaagcgtggg cgaaaacccg ccaaaaagca gcggaaaaaa
     6181 tccgtgatgt ggcggcagaa ttgcttgatg tttatgccaa acgagaaagc caaaccggtt
     6241 ttgcgtttgc ctacgataaa gcggaatttg atcagtttag tgccactttc ccttatgagg
     6301 aaacggacga tcagaaaatt gccatcaatg ccgtaattag cgatatgtgc caagccaaag
     6361 cgatggatag attggtgtgt ggcgatgtcg gcttcggcaa aaccgaagtg gccattcgag
     6421 cgactttctt ggcagtgatg aaccataaac aggtggttat tcttgcccca actaccttgc
     6481 tcgcccaaca gcattatgag aatttcaaag accgtttcgc caattaccct gtgaatgtgg
     6541 aagtgctgtc tcgctttaaa accgccaaag agcagaaaac gattttgcaa aatgtggcgg
     6601 agggcaaagt ggatattttg gtcggcacgc acaaactgtt gcaagacgat gtagcgttcc
     6661 gtgatcttgg gcttttagtg attgatgaag aacaccgttt cggcgtacgc caaaaagaga
     6721 aaatcaaaca actgcgtgcc aatgtggata ttctgaccct caccgccacg ccaattccaa
     6781 gaacgctgaa tatggcactc aacggaatgc gtgatctctc gattatcgcc agcccgccgg
     6841 ctcgccgact tttaatcaaa acctttgtgc gacaaagtga tgaaatggta atccgtgagg
     6901 caattttgcg tgaaattctg cgtggtgggc aggtttacta cctccataac gatgtggcaa
     6961 ccattgaaaa ttgtgccgag aaattggctg aactcgtgcc agaggcaaga attgtgatcg
     7021 gacacgggca gatgcgtgag cgtgaattgg agcgggtgat gagcgatttc tatcatcaac
     7081 gctttaacct gctggtttgc tccaccatta ttgaaacggg gattgacgtg ccgactgcca
     7141 ataccattat tatcgaacgg gcggacaaat tcgggctggc acaactgcac caactgcgtg
     7201 gacgggtcgg acgctcgcac caccaagcct acgcctatat gctcacaccg cacccgaaaa
     7261 cgctcaccaa agatgccgag caacgcttgg aggcgatgag taccattgat aatctcgggg
     7321 ccggttttgc gttggcgacc cacgatttag aaattcgtgg tgcaggcgaa ttgctcggtt
     7381 cggagcagag cggacagatt gaaagcatcg gtttttcgct ctatatggac ttgctcgaaa
     7441 atgcggtcaa agctttgcaa gaagggcgag aaccgagcct cgatgaaatc acccaacacc
     7501 aagcggagat tgaattgcgt gtaccggcct tattgccgga cgattatctg cctgacgtga
     7561 atatgcgtct gtcgttctac aagcggatcg caagctcgga aaatctgcaa agcctgaacg
     7621 acttgaaagt ggagctgatc gatcgcttcg gcttgctccc agaggcaacc aaaaacctgt
     7681 ttgcgataac ccaacttcgc ttgtcggcac aaacgctcgg gattaagaaa atcgatgccg
     7741 gcattcacgg cggttatctt gaatttaaac cgacagccca gcccgaccca atgaagttta
     7801 tcgccttaat ccaaaaacaa ccagcggtgt acagttttga aggggcggtg aagttcaaat
     7861 tccgcttagc actcgaagac aaccgcaagc ggttagaatt tgtggaaaat ttgctaaggg
     7921 aggttgtggg cggataaagc ctcctaaggt tgtcaatctt gaaaaacgat ttttgcgatc
     7981 tgtatcgtaa aaatcgtttt tgtttattgc gaatccagta atttcatcta ggctgtttat
     8041 aacttaaaaa aatagatgta aatatatttt atttacaatc aaaaaggagt aaactggcaa
     8101 tggaagaaat taatggtttt gaatgggata gtgagaaagc tcttcgtaat caacaaaaac
     8161 acggagttag ttttatcgag gcggtaagcg tattttatga taataatgcg atgttaatcc
     8221 cggatccaga tcactcatct agggaagagc gttttttgct actcgggcgt agcaataaag
     8281 ataatctact tgtggttgtg cattgcgaga gaaataccaa cattcggatt atttcagcac
     8341 gtttggcaac aaaacgcgaa agtgggcaat ataaaggagt acattaaatg agagatgaat
     8401 acgatttttc taatgcagtg aaaaaccctt acgcaaaagg gctaaaaagc aaaagtgtgg
     8461 ttactattcg gattgaagac gatgttatca gctattttaa acaacttgcc gaagaggtga
     8521 atttgcctta ccaaacgttg atcaatcttt atttgaaaga ttgcgttgag cagaaacgta
     8581 aaccgactat tagttggtaa tattatgatt tgaaatcagt atgttattga gtatttgaga
     8641 atgggttatc ttgccagttg tataaaatag gcatatactt tggcatatac actatctatc
     8701 ggagggtaaa gtaagatgga aaaaattgcg agattattcc gtaacgggcg gaaccaagca
     8761 gtgcgtttac cggttgaatt tgagtttgat gcagagcaag tctatgtttc taaaaaagaa
     8821 aatggcgata ttttattatc attgagatcg agaaataccg agcattggca acgattgttt
     8881 gagctattgc ctgctgttgt atgtgatgaa acatttttag atacaaaaga gagaaaccaa
     8941 tcctttgagc aacgggatcc ttttgaggta gcttaaatgt atatgttaga tacgaatact
     9001 gtaagctatt tttttcgtgg aaatgcaaat gtggtggcga aattgaagtc cattaatcct
     9061 gagaggcttt gtatttcatc tgtaactgct gctgaattgg tttatggtgt ggcgaaaaga
     9121 aataatgggc agctttctca atttttagat tattttttat cgagtgtaaa ggttttagat
     9181 tggaattatc gttgtgctga actttatgga aagttacggg cagagatgga gaaaaatggc
     9241 aaagtaatgg gcgtgcagga tcaaatgatt gctagtcacg ctcttgctga agagtgtatt
     9301 ttagtttcta gtgatcaggc atttaaaatg gtacctgatc tgaaattgga gaattggtta
     9361 cactaaacta tagtaaggaa aaacaatgaa acaatccctt cttaaccgtt tactacgtta
     9421 cacccaaatt cacacaactt cagatccgaa aagccaaact gtgccaagct cgcaacagca
     9481 gtgggatttg cttcatctgc tgaaaacgga agtagaggcg atggggctaa gcgagattta
     9541 tcttgacccc aacggctatc tgttcgccag cttgcctgcc aatattgaca aaaccctgcc
     9601 ggtgcttggg ctgattgccc acgttgatac ttcgcccgat tttaatggtg aaaacgtgaa
     9661 accacaggtg attgaggctt atcaaggcgg caatattttg ctaaacggca gtggcgatta
     9721 tctttcgcca acggatttcc cgaatttaaa caagctgatt ggcgacacct tagtcaccac
     9781 tgacggcacc tctttgcttg gcgcagacga caaatccggc attgcgatta ttctctcggc
     9841 tatcgagtat ttacaagcca atccgcatat tccgcacggc aagatccgca tcggctttac
     9901 caccgatgaa gagatcggtc gaggcgcaga tcattttgat gtaaacgcat tcggtgccga
     9961 tgtcgccttt acctttgatg gcgggcctct gggcgaattg caatatgaaa atttcaatgc
    10021 cgatgcagcg accgttacgt tcttcgggcg tagcgttcac cccggcacgg caaagggcaa
    10081 gatgatcaac gccttagagc gagcttgcga gttccaacac cgtctgcctc cccatttaac
    10141 ccctgccacc agcgaagggc gtgaaggctt tattatgttg cacggcattg agggtggaat
    10201 tgataaagcg gaaattcact atattttacg tagctttgag cgtgatgaat tagctgcatt
    10261 cggcaaaatg ctgaatgata cgctggcaga gatgcaacag atttacggcg aacattgcgg
    10321 aaaagtcgag attgtcgagc aatatcgcaa tatgcgtgaa gtgctggacg attacggcta
    10381 cttggttgaa attgccgaaa acgcaatgcg tgaacaaggc attgagccga atttagaacc
    10441 gatccgtggc ggtactgatg gctcacgcct ttcatttatg ggcttgcctt gcccgaattt
    10501 atttaccggc ggcgaaaatt tccacggacg atttgagttt atttcggttg atacgatgga
    10561 aaaagccaca aaagttgtaa ttgatatttg tcggaaattt gctgattatg atttcaacaa
    10621 ataaggcggc tgcaagcggt caaatttgat gaaaaatttg caaaaagcgt tatttttcac
    10681 caattaaacg attttttaca gatttcctac aaatttattt gacattaaat ctgaaatcca
    10741 tagaatatgc acctgttgag acctctacga ggttcgtagc gtttttaa
//
LOCUS       DANLYI010000070        10659 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.070, whole genome shotgun sequence.
ACCESSION   DANLYI010000070 DANLYI010000000
VERSION     DANLYI010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 10659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10659
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.070"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            complement(8..868)
                     /gene="pdxY"
                     /locus_tag="PXD81_001299"
     CDS             complement(8..868)
                     /gene="pdxY"
                     /locus_tag="PXD81_001299"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005721193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase PdxY"
                     /protein_id="HDL5537322.1"
                     /translation="MKNILSIQSHVVYGYAGNKASTFPMQLLGVDVWALNTVQFSNHT
                     QYGKWGGMVMPKEQIGSIVDGIHNIGELHRCDAVLSGYIGSAEQVAEIIQAFHKVKAE
                     NPNAIYVCDPVMGHPDKGCIVADGVKEGLINLAMAQADIITPNLVELRELTGLTVENF
                     EQAVEAVKVILTKGPKKVLVKHLSRVGKESNKFEMLFANQDGIWHISRPLYQFAKEPV
                     GVGDLTAGLFVANLLNGKSDLEAFEHTANAVNEVMEQTARSGLYELQIIASRDRIVNP
                     QCSYQAVKIA"
     gene            987..1062
                     /locus_tag="PXD81_001300"
     tRNA            987..1062
                     /locus_tag="PXD81_001300"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1020..1022,aa:Thr,seq:tgt)
     gene            complement(1157..2110)
                     /gene="accA"
                     /locus_tag="PXD81_001301"
     CDS             complement(1157..2110)
                     /gene="accA"
                     /locus_tag="PXD81_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005598724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HDL5537323.1"
                     /translation="MSQEFLDFELPIAELEAKIESLRAVSEQDDKINLDDEIKRLQKK
                     SEELTKKTFANLDAWQISRMARHPNRPYTLDYIEHIFTEFDELAGDRAFADDKAIVGG
                     IARLDGRPVMVIGHQKGRTVKEKVKRNFGMPAPEGYRKALRLMEMAERFNMPIITFID
                     TPGAYPGIGAEERGQSEAIARNLREMSQLSVPVICTVIGEGGSGGALAIGVGDKVNML
                     QYSTYSVISPEGCASILWKSADKASTAAEVMGLTAPRLKELELIDNIVPEPLGGAHRN
                     VEQIAQNLKQRIVEDLAGLDQLNKEDLLERRYQRLMNYGYV"
     gene            complement(2164..3588)
                     /locus_tag="PXD81_001302"
     CDS             complement(2164..3588)
                     /locus_tag="PXD81_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HDL5537324.1"
                     /translation="MKKQITRRQFLQGSALAAGFATVPTPLLAAERPQLNIPPLMDIG
                     RGKPVRLDLRPAQTQFDKGKLVDVWGVNGQYLAPTVRVKSGDFVKLTYLNNLPQKISM
                     NIQGLQASSEMIGTVHRSLDPKSSWSPIVSINQPACTCWYHADTMLNSAFQVYRGLAG
                     LWMIEDEQSKKATLPNKYGVNDIPLILQDQLLNKDGVQVLDKNTSQFFGKRLFVNGQE
                     SPYFNVPRGWVRLRIVNASLSRAYELRLDNEQPLYLIATGLGMLAEPLETESVQLAPS
                     ERVEVLVDLSEGKAVSLISGQKRDIFYKIGQVFADNDELNDNVILELRPEGMAAVLNN
                     KPSLPAFNLEEFNLKISQERRFAIRPADYLINQKRFDPKRIDFTAQKGSVERWYLHST
                     QAVGFTLQGAKFIIETLNRKQYPIKQLAWRDTVWLEAEQEITLLVRFDNTAPEQLPFT
                     FGVSDFMLRDRGTMGQFSVIENGQ"
     gene            complement(3590..4324)
                     /locus_tag="PXD81_001303"
     CDS             complement(3590..4324)
                     /locus_tag="PXD81_001303"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HDL5537325.1"
                     /translation="MLKIFRILIVALAAIFISIFGTIYALLRLRHPSSVGVVARWFGA
                     LHKLVGLELITRKKPEINYPVVYIGNHQNNYDMVTISSMVAEKTVSIGKKSLIWIPFF
                     GLVYWATGNIFIHREKRSSAIETMNKAGQIVKERNVSVWMFPEGTRSRGRGLLPFKTG
                     AFHLAISAGVPIVPVVCSNLHNKVDLNRSNNGTVICEMLDPIDTSGYSRENVKELMEK
                     CHQIMLAKFEQLNQEVEQLENTAKNA"
     gene            4471..4968
                     /gene="tpx"
                     /locus_tag="PXD81_001304"
     CDS             4471..4968
                     /gene="tpx"
                     /locus_tag="PXD81_001304"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005578656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HDL5537326.1"
                     /translation="MSKVTLAGSPIDVAGTFPQKGDVVADFTLVNSDLSDVSLSDFDG
                     KRKVLNIFPSIDTGICATSVRVFNQKAADVANTVVLCISADLPFAQARFCGAEGIENA
                     KVLSSFRHRDLHNKLGVDIISGPLAGLTSRSVIVLDENNKVLHSELVPEIKQEPDYEA
                     ALAVL"
     gene            complement(5053..6678)
                     /gene="yidC"
                     /locus_tag="PXD81_001305"
     CDS             complement(5053..6678)
                     /gene="yidC"
                     /locus_tag="PXD81_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012263229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HDL5537327.1"
                     /translation="MNNRGLLLMGLFLVSILIFTQWQQDFDPEIQAQKQAQVAAQTQQ
                     AGDLPSASNANNPIADSITQGKTITVESDVLRLTIDTLGGDVIGSDLLKHNAELHSNT
                     PFKLLQQDGDKIYVAQSGLVGKNGIDTNAGRPQYAVTADSFKLAEGQNEISVPLTFEK
                     EGVVYTKTFTLKRGSYDVGVNFNIKNNTAQAIEVQPYGQLKHSIVESSGNLTMPTYTG
                     GAYSSSETNYKKYSFQDMEKANLSVDTKAGWIAVLQHYFVSAWVPNQDADNSLYSRTN
                     NGVATIGYRGPLTTIAPNSEANLNSKLWTGSKDQKEMGESANHLDLTVDYGWAWFIAK
                     PLFWLLTAIQKIVSNWGLAIIGVTIVVKTILYPLTKAQYTSMAKMRMLQPRITEMRER
                     FGDDRQRMSQEMMKLYREEKVNPMGGCLPILIQMPIFIALYWTFMEAVELRHAPFFGW
                     IQDLSAQDPYYILPLLMGGSMFLLQKMSPTPVADPVQQKVMTFMPVIFTVFFLWFPSG
                     LVLYWLTSNLITIAQQWLIYRNLEKKGLHSRKK"
     gene            complement(6831..7256)
                     /locus_tag="PXD81_001306"
     CDS             complement(6831..7256)
                     /locus_tag="PXD81_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005598632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog fold thioesterase"
                     /protein_id="HDL5537328.1"
                     /translation="MTIWKQTATCEQLNTLSQKSAVSHLGIEFTAIGEDWIEAQLQVD
                     ERTQQPFGVLHGGVSAALAETTANAGALMVCEPHQIAVGMELNISHLKSIPAGTKAIA
                     KATPLKLGREVQVWNIDIKDEQGNLCAVARLSTKTLDKR"
     gene            complement(7585..10647)
                     /locus_tag="PXD81_001307"
     CDS             complement(7585..10647)
                     /locus_tag="PXD81_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005820672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HDL5537329.1"
                     /translation="MDKATQSYLTELVNQHFSGEIASNYADRLSLATDNSVYQQIPQA
                     ILFPKSVSDVVILTKLAQKPEYQHLTFTPRGGGTGTNGQSLNNNIIVDLSRHMNQILE
                     LNVEERWVRVQAGVVKDQLNQFLKPHGLFFSPELSTSNRATLGGMINTDASGQGSLQY
                     GKTSDHILAIKSVLINGDILETQAVKSADFFAHIEGLNLSALGKTLHREIFNRCKTLR
                     PKVLNELPQLNRFITGYDLKNVFNHGESEFNLTRILTGSEGSLAFICEAKLNLLPIPK
                     YRTLINIKYNSFDAALRSAPFMLEAKALSVETVDSKVLNLAKQDIVWHSVSDLLTEDP
                     ANPILGINIVEYASSDQTQIETQVKALCQSLDQKMANNEAGIIGYQLTSDVNSIEKIY
                     AMRKKAVGLLGNAKGWAKPIAFVEDTAVPPENLADYIVEFRALLDRYNLPYGMFGHVD
                     AGVLHVRPALDLCDKAQVETFKQISDQVVELTAKYGGLIWGEHGKGMRSYYGERFFGE
                     TLWQELRYIKTLFDPYNRLNPGKICTPLESETSLYPIDSQMRADFDRQIPIQVREEFK
                     GAMNCNGNGLCFNFDVHSTMCPSMKVSGNRLYSPKGRATLVREWLRLLTEQNVSPDDL
                     IFKPRKQAVKLADFVAKIRNTLNKNKEYDFSHEVKAAMDTCLACKACASQCPIKIDVP
                     TFRSQFNELYHSRYLRPLKDYVVSNLEFIAPLMAKQPRFFNFFSTSTFAEKAANKTIG
                     MTYLPALSVPNLQHQLVEVGYQGESLESLEQQATSGDFSAKNCKTLFIVQDPFTSYYD
                     AKVVADFIALIKKLGFKPIVLPFKPSGKAQHVKGFLHKFAKTAKNQADFLNRVAKLGL
                     PMVAVDPALTLLYREEYKEILKEARGDFKVLLAHEWLREVIKNGELENCKKSANSDRL
                     PWYLFAHCTESTALPSSMKEWQAIFTHFGETLLNENVGCCGMAGTFGHETKHVEMSKA
                     IYQQSWQIKLKNKPLERCLATGYSCRSQVKRFEQQGIKHPIQALLDVI"
BASE COUNT         2836 a         2285 c         2287 g         3251 t
ORIGIN      
        1 catttaatta ggcaatcttt acagcttggt aactacactg tggattaaca atccgatcac
       61 gagaagcaat aatttgtaac tcataaagcc ctgatctagc ggtttgttcc attacctcat
      121 tgaccgcatt tgcagtgtgt tcaaaggctt ctagatcgga ttttccattt aataagttcg
      181 ccacaaatag ccctgcggtt aaatcgccta caccgacagg ttctttggca aattggtaga
      241 gtgggcggct gatgtgccaa atgccatctt ggttggcgaa cagcatttca aatttgttgc
      301 tctctttgcc gactcggctt aaatgtttaa ccaatacttt tttcgggcct ttggttaaaa
      361 tcactttcac tgcctcgacc gcttgctcaa aattttccac cgttaaaccg gttaattctc
      421 ttaattccac taaatttggt gtgataatgt cggcttgtgc cattgccaga ttgatcaatc
      481 cctcttttac gccgtctgcc acaatgcagc ctttgtcggg gtgtcccatt accgggtcgc
      541 atacataaat tgcgtttgga ttttccgctt tcactttatg aaaagcctga ataatttcgg
      601 ctacttgttc tgccgagccg atatagccag agagtacggc atcgcaacga tgcagttcac
      661 cgatattgtg aatgccatca acaatgctac cgatttgttc ttttggcatc accataccgc
      721 cccattttcc atattgggta tggttggaaa actgtacggt atttaacgcc catacatcaa
      781 cacctaacag ctgcatagga aaagttgaag ccttattgcc ggcatagccg tagacaacgt
      841 gcgattgtat ggatagtatg tttttcattc attcactcct ttaaaatttc aatatgtgtt
      901 gttttttagt catttgaagt tgcaaggact tgaagctcat caagaaatct ctataatccc
      961 gcctcgttct cttatccatt caatatgccg acttagctca gtaggtagag caactgactt
     1021 gtaatcagta ggtcaccagt tcgattccgg tagtcggcac catcaccttt attttctgat
     1081 gagtcccctt taatgtaaaa tggcaagatt ttgcaaattt tcacaaaaat cttaccgctt
     1141 gtagggtgaa attgaattag acgtaaccgt aattcattaa gcgttgataa cggcgttcca
     1201 gtaaatcctc tttattaagt tgatctaacc ctgccaaatc ttcaacaata cgctgtttaa
     1261 ggttttgggc gatttgttca acattgcgat gagcaccgcc taagggttcc ggcacgatgt
     1321 tatcaatcag ctcaagctct tttaaacgag gggcagttaa acccattact tctgctgccg
     1381 ttgaggcttt atcggcactt ttccacagaa tcgaggcaca gccttcaggt gaaattaccg
     1441 agtaagttga atattgcaac atattgacct tatcgcccac accgatcgct aatgcaccgc
     1501 ccgagccacc ttcgccaatt accgtacaga ttactggcac ggaaagttga gacatttcac
     1561 gcaggttacg ggcaatcgct tctgactgac cacgctcttc cgcaccaatg cccggataag
     1621 ctcccggtgt gtcgataaag gtgatgattg gcatattaaa gcgttctgcc atttccatta
     1681 aacgtaacgc tttgcgatag ccttcaggag caggcatacc aaagttacgt ttcactttct
     1741 cttttacggt acgacctttc tgatgaccga taaccatcac cggacgtccg tccagacggg
     1801 caataccacc gacaatcgct ttatcatcgg caaaggcacg gtcgcctgcc agttcgtcaa
     1861 attcggtgaa aatatgctca atataatcta aagtgtacgg gcgattgggg tggcgtgcca
     1921 tacgggaaat ttgccacgca tctaaattag caaaggtttt cttggttaat tcctcgcttt
     1981 tcttttgtaa acgcttgatt tcatcatcaa gattaatttt gtcatcttgc tcgctgactg
     2041 ctcttaaaga ctcgatcttg gcttccaatt cagcaatcgg caattcaaaa tctaaaaatt
     2101 cttggctcat tctattttcc tttttatggg caaaattatg gggcattatc gcttatcagt
     2161 cggttattgt ccattttcaa ttacgctaaa ttgacccatc gtacctctat cacgcagcat
     2221 aaaatcgctc acgccaaagg taaatggcag ttgctcaggg gcagtattat caaagcgaac
     2281 caacagcgtg atttcttgct ccgcctcgag ccaaacggtg tcccgccacg caagttgttt
     2341 gatagggtat tgcttgcggt ttagggtttc aataatgaat tttgcacctt gtagcgtaaa
     2401 tccgaccgct tgtgtgctat gtaaatacca acgctcaacg ctgccttttt gcgcggtaaa
     2461 atcaatgcgt tttgggtcaa accgcttttg gttgattaaa taatcagccg gacgaatggc
     2521 aaaacgtctc tcttggctga ttttcaggtt aaattcctct aagttgaaag ccggtaaact
     2581 cggtttatta ttcaataccg ctgccattcc ttcagggcga agctctaaaa tcacgttatc
     2641 attcaattcg tcgttgtcag caaagacctg cccgattttg taaaaaatat ctcttttttg
     2701 accgcttatc agcgataccg ctttcccttc gcttaaatcg accaacactt ccaccctttc
     2761 actcggtgca agttgaaccg attcggtttc aagtggctct gccagcattc ctaagccggt
     2821 ggcgattagg tacaacggct gctcgttatc taagcgtaat tcataagcac gggaaagtga
     2881 ggcattgacg atccgcaatc tgacccagcc acgaggcacg ttaaaataag gcgattcctg
     2941 cccgttgaca aataatcgct taccgaaaaa ttgtgaggtg tttttatcta acacctgtac
     3001 gccgtctttg ttgagcagct ggtcttgcag aattaacgga atgtcgttca cgccatattt
     3061 attcggtaaa gtggcttttt tgctctgttc atcttcaatc atccacaagc cggctaagcc
     3121 acgatacact tggaatgccg agttgagcat tgtatcggcg tgataccaac aagtacaagc
     3181 cggttgattg atagaaacaa tcggcgacca gctacttttg gggtcaaggg aacgatgcac
     3241 cgtgccaatc atctcacttg aggcttgcaa tccttgaata ttcattgaga ttttttgcgg
     3301 taaattattg agataagtta atttgacaaa atcccccgat tttacccgta cggttggggc
     3361 aagatattgc ccgttcacac cccatacatc aaccaatttg cctttatcaa attgggtttg
     3421 tgcggggcgt aaatccaacc gaaccggctt tcctcgccca atatccatta aaggcggaat
     3481 gttcagttgc ggtcgctctg ctgctaatag cggtgttggc actgttgcaa atccagccgc
     3541 aagggcagag ccttgtaaaa attggcgtcg ggtgatctgt tttttcatat taggcgtttt
     3601 ttgcagtgtt ttctaactgt tcgacttctt gattaagttg ttcaaatttt gccaacataa
     3661 tttgatggca cttttccatc agttctttga cgttttcacg ggaatagcct gaagtatcaa
     3721 tcggatcaag catttcacag attactgtgc cgttatttga acggttcaaa tccactttat
     3781 tgtgtaaatt agagcaaact accggcacaa tcggcacgcc ggcagaaatg gctaaatgga
     3841 atgcaccggt tttaaatggc agtaaaccac gcccacggct acgagtacct tccgggaaca
     3901 tccacaccga cacattgcgt tctttcacga tttgccctgc tttattcatt gtttcaatcg
     3961 cactggaacg cttttcacgg tgaataaaaa tattgccggt tgcccagtaa accaaaccga
     4021 aaaacggaat ccaaatcagg ctttttttgc cgatactcac cgtcttttct gccaccattg
     4081 aggaaatcgt taccatatcg taattgtttt gatgattgcc gatatagaca accggatagt
     4141 taatttccgg ctttttgcgg gtaattaatt ccaacccgac taatttatgc aaagcaccaa
     4201 accaacgagc taccacaccc acgctacttg ggtgacgtaa acgcagtaac gcataaatag
     4261 tgccaaaaat agaaatgaaa atagcggcaa gggcaacaat taaaatacga aaaattttta
     4321 acataatgga ataaccttaa aaataaattg tctaggagta tagcaaaata aggttatttt
     4381 ctaaaggcta tccacattaa attgatagtt tcctattttg caaaaaatcg gtatatttaa
     4441 accgcttgtt tatttgataa ggagaaaaaa atgagtaaag tgactttagc aggaagcccg
     4501 attgatgttg caggcacatt tccacaaaaa ggcgatgtgg ttgccgattt taccttagtc
     4561 aacagtgatt taagtgatgt ttcgctttcc gattttgatg gcaaacgcaa agtgttaaat
     4621 attttcccaa gtattgacac cggcatttgt gccacttctg tgcgtgtgtt taatcaaaaa
     4681 gcggctgatg tagcgaatac ggttgtcctt tgtatttcag cggatttacc ttttgcccaa
     4741 gcacgtttct gtggagcaga aggaattgaa aatgccaaag tattatccag tttccgtcat
     4801 cgtgatttac acaacaaact gggggtagat attatttcag gtccgttggc aggcttaact
     4861 tcacgttcag taattgtgtt agatgaaaac aacaaggtat tacacagcga attagtgccg
     4921 gaaattaaac aagagccgga ttatgaggcg gcgttagcgg tattataatt tgcagaatta
     4981 tgctggaaaa taaccgcttg taaagcaaca aaaagcccgt tgattcaatt caacgggctt
     5041 tgtttttagt gattatttct tacgagagtg taagcctttt ttctcaaggt tacggtaaat
     5101 caaccattgc tgtgcaatcg tgattaagtt tgaggttaac cagtaaagca ctaagcctga
     5161 tgggaaccat aagaagaaca cggtaaagat aaccggcata aaggtcatta ctttttgttg
     5221 taccgggtca gctaccggtg ttggcgacat tttttgtaac aagaacatcg aaccacccat
     5281 taataaaggc aaaatgtagt atgggtcttg tgccgataag tcttgaatcc agccgaagaa
     5341 aggtgcgtgg cgtaattcta ccgcttccat aaatgtccaa tataaagcaa tgaaaatcgg
     5401 catttggatt aaaattggta agcagccacc cattgggttc actttttctt ctctgtagag
     5461 tttcatcatc tcttggctca tacgttggcg gtcgtcgccg aaacgttcac gcatttcagt
     5521 aatgcgaggt tgtaacatac gcatttttgc catcgaggtg tattgtgctt tggttaatgg
     5581 gtaaagaatg gttttcacta caatagtgac accgataatt gccaagcccc agttgcttac
     5641 gattttttga attgcggtta gtaaccagaa aagcggttta gcgataaacc acgcccagcc
     5701 gtaatctacg gttaagtcta ggtgatttgc tgattcgccc atttctttct gatctttaga
     5761 acctgtccaa agtttgctat ttaaattagc ctctgaattt ggcgcaatcg tagttaaagg
     5821 gccacggtaa ccgatagttg ctaccccatt attggtgcgt gaataaaggc tgttatccgc
     5881 atcttggttt ggcacccaag cagacacgaa atagtgctgt aataccgcaa tccaacccgc
     5941 tttggtgtcc acgcttaagt tggctttttc catatcttgg aaactgtatt ttttgtagtt
     6001 ggtttctgaa gaagaataag ccccgccggt gtaagtcggc atggttaaat tgccggaact
     6061 ttcaactata gagtgtttta attgcccgta aggctgaact tcaatcgctt gtgcagtatt
     6121 atttttgata ttgaagttta ccccaacatc gtagctacca cgttttagcg tgaaagtttt
     6181 ggtgtaaacc acaccctctt tttcaaaggt taatggtaca gaaatttcat tttgcccttc
     6241 ggctaatttg aagctatctg ccgttaccgc atattgtggg cgaccggcat tggtatcaat
     6301 cccgttttta cccactaaac cgctttgagc aacgtagatt ttgtcgccgt cttgctgtaa
     6361 tagtttaaat ggcgtgttag agtgtaattc agcattgtgt tttaacaaat ctgagccgat
     6421 gacatcaccg cctaaggtat caatagtcag acgtaataca tcgctttcca cggtaatggt
     6481 tttaccttgc gtgatgctat ctgcgattgg attattggca ttggaagcag acggaagatc
     6541 acccgcttgt tgggtttgtg ctgccacttg agcttgtttt tgtgcttgaa tttcaggatc
     6601 aaaatcttgt tgccattgtg taaaaatcaa aatagacaca agaaataaac ccattagcag
     6661 taagccacga ttattcatgt tggaattctc ttgttagtaa tggataaaaa gaaaggtatt
     6721 ttataatagg ttagcttgtt ttccaaacgc cttttatgaa caagcggtcg gattttccaa
     6781 gttttttgca aaattttcgg agaatttgac cgcttgtgga gaataagaaa ctagcgttta
     6841 tctaaggttt tggtggaaag gcgagcaact gcacacaggt taccttgttc gtctttaatg
     6901 tcgatattcc aaacctgtac ttcacgccct aatttcagcg gtgtagcttt ggcaatcgct
     6961 tttgtgcctg ccggaatcga ttttaaatgg ctgatgttta gctccattcc caccgcaatt
     7021 tgatggggtt cacacaccat taacgcccct gcattggcag tagtttccgc gagtgctgcc
     7081 gatacaccgc cgtgcaatac gccaaacggc tgttgagtgc gttcgtccac ctgaagctgt
     7141 gcttcaatcc aatcttcacc gatagcggta aattcaatcc ctaagtgtga caccgccgat
     7201 ttttggctga gtgtgtttaa ttgttcacaa gttgctgttt gcttccaaat ggtcattatt
     7261 atttcctgtc taataaaaaa gctgtctatt tagacagctt tcattatctc tttgagcgaa
     7321 acatactacg ccaatagtac attgtttggt cgcccatatt accttaagcc atatagcact
     7381 aaggaggtga tttatgtgag aaaatcagaa gttttctttc tggttgcgtt gctaatatta
     7441 ttagcaactt ccgctagcat taaagtactc accatacttg atgtgctagt ttggttattc
     7501 gctagataac ctaaaatcag acagaggcag gctggttacc tgcctcgctt tttattctaa
     7561 gtacttttag gttacatttc aaccttaaat cacgtccagc aacgcttgaa ttgggtgttt
     7621 aatgccttgt tgttcaaaac gtttgacttg cgagcggcag gagtagccgg ttgctaggca
     7681 acgctcaagc ggtttatttt ttaatttaat ttgccatgat tgctgataaa tcgctttcga
     7741 catttcaacg tgtttggttt cgtgtccgaa cgtgcctgcc attccacagc aaccgacatt
     7801 ttcattgagt aaggtttcac caaaatgggt gaaaatagct tgccactctt tcattgagct
     7861 tggcaaggcg gtggattcgg tgcagtgagc aaataaatac cacggcaagc ggtcggaatt
     7921 tgccgatttt ttgcaatttt ccagctcgcc gtttttaatc acttccctta accattcgtg
     7981 tgccaataag actttgaaat ccccccgagc ttctttgagg atctctttgt attcttcacg
     8041 ataaagcaaa gttaaggcag gatcgacagc caccatcggt agccccaatt tcgccacacg
     8101 gtttaaaaaa tcggcttggt ttttcgctgt tttcgcaaat ttatgcaaaa agcctttaac
     8161 gtgttgtgct ttaccgcttg gcttgaatgg caacacaatc ggcttaaagc ctaatttttt
     8221 aatgagagcg ataaagtctg ccaccacttt ggcatcgtaa taagaggtaa acggatcttg
     8281 cacaataaac aaggttttgc aattttttgc agaaaaatca ccgcttgtag cttgttgttc
     8341 aaggctttct aagctctcgc cttggtagcc gacttccacc aactgatgtt gcagatttgg
     8401 cactgacaat gccggcaaat aggtcattcc aatggttttg tttgcagctt tttcggcaaa
     8461 agttgaggtg ctgaaaaagt taaaaaaacg tggttgtttc gccattaaag gggcgataaa
     8521 ttctaagttt gagaccacat aatctttcag tggtcgcaaa tagcggctgt ggtaaagctc
     8581 gttaaattgc gatctgaacg tcggtacgtc aattttgatt gggcattggc tggcacaggc
     8641 tttgcaggca aggcaggtgt ccatcgccgc tttgacttcg tgtgaaaaat catactcttt
     8701 gtttttattg agcgtgttgc gaatttttgc aacaaaatca gctaatttga ccgcttgttt
     8761 gcgaggtttg aagatcaaat cgtccggcga aacgttttgt tcggtaagta atctgagcca
     8821 ttcacgcaca agagtagccc gccctttcgg tgagtaaaga cggttgcccg agactttcat
     8881 tgaagggcac atcgtgctgt gtacatcaaa gttaaaacat agcccgttgc cgttacaatt
     8941 cattgctcct ttaaattctt ctcgcacttg gattggaatt tggcggtcaa aatcggctcg
     9001 catttgtgag tcaatcgggt agagcgaggt ttcgctttct aatggtgtac aaattttacc
     9061 tgggttcaag cgattgtacg ggtcaaataa ggttttgatg taacggagtt cttgccacaa
     9121 agtttcgcca aagaaacgtt cgccgtagta agaacgcata cctttgccgt gttcgcccca
     9181 aatcaagcca ccgtatttgg cggtgagttc gactacttgg tcggaaattt gcttaaaggt
     9241 ttccacttgg gctttgtcgc ataagtcaag agcagggcga acgtgtagca cgccggcatc
     9301 aacgtggccg aacattccgt aaggcagatt atagcgatct aataaagctc gaaattccac
     9361 gatgtaatcg gcaaggtttt ccggtggcac agcggtgtct tctacaaagg cgatcggttt
     9421 tgcccagcct ttggcattgc ctagcaggcc tacggctttt ttccgcattg cgtagatttt
     9481 ctcgatagaa tttacatcac tcgtgagttg gtagccgata atcccggctt cattatttgc
     9541 cattttttga tcaaggcttt ggcagagtgc tttcacttgg gtttcaattt gagtctgatc
     9601 gcttgaggca tattcaacaa tgttaatccc taaaatcggg ttggctgggt cttcggttaa
     9661 taaatctgac accgaatgcc agacgatatc ttgcttggca agattgagca ctttggaatc
     9721 aaccgtttcc accgataagg cttttgcctc taacataaaa ggagcggagc gtaaggcggc
     9781 atcaaaggaa ttatatttaa tattaattaa ggtgcgatat ttgggaatcg gcagtaaatt
     9841 cagtttggct tcgcaaataa aggcaagcga accttcagaa ccggttaaaa tacgggtaag
     9901 gttaaattcg ctttcaccat ggttaaacac attttttaaa tcatagccgg taataaaacg
     9961 gttaagctga ggcagttcgt tcagcacttt ggggcgtaat gttttgcaac ggttgaaaat
    10021 ttcacggtgc aaggttttgc cgagagcgga gagatttaac ccttcaatat gtgcaaaaaa
    10081 atcggcagat ttgaccgctt gtgtctctaa aatatcgccg ttaataagca cagatttgat
    10141 ggcaagaata tgatcagatg ttttgccata ttgcaaagaa ccttgcccag aggcatcggt
    10201 gttgatcatt ccgcctaaag tcgctcggtt actggtagaa agttcggggg aaaagaaaag
    10261 tccatgtggt tttaaaaatt gattgagctg gtctttcacc acgccagcct gcactcgaac
    10321 ccaacgctct tccacattca gttccaaaat ctgattcata tggcgggaga gatcaacaat
    10381 aatattattg ttaagagatt gcccgttagt gcctgtgcca ccgcctcgtg gagtaaaagt
    10441 taaatgttga tattctggct tttgggcaag tttggttaga attacgacat cagaaacgga
    10501 tttcggaaac agaatagctt gcggaatttg ctgatacacg ctgttgtctg ttgccagtga
    10561 aagcctatct gcataatttg atgcaatttc cccactaaaa tgctgattga cgagttcagt
    10621 taaataggat tgggtggctt tatccagttg cggtacttc
//
LOCUS       DANLYI010000071        10625 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.071, whole genome shotgun sequence.
ACCESSION   DANLYI010000071 DANLYI010000000
VERSION     DANLYI010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 10625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10625
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.071"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            57..299
                     /locus_tag="PXD81_001308"
     CDS             57..299
                     /locus_tag="PXD81_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015433650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL5537330.1"
                     /translation="MSDKSIILEIINALGNKENIKQVEACLTRLRVVLHNNGLLNKSA
                     LKKLGAVDVIKVGDTQQIIFGAKAGQYRDEINALIG"
     gene            complement(343..1167)
                     /locus_tag="PXD81_001309"
     CDS             complement(343..1167)
                     /locus_tag="PXD81_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDL5537331.1"
                     /translation="MNGPTQLAHFQEQIRKEYDGLSKRLKQVAQYVLDNGNSVVFDTV
                     STIAERADVPPSTLIRFANAFGFSGFNEIKQLYRQDMMEGTSRYTERVQLFRQIEPLD
                     EQNSELNHILDVFVQGNTQALQQLSTQVSEEQLAQTVDILHNAKRIFIVGLKRSFSIA
                     NYLNYALHHLDYDVFMIDGSGGMFDEQLSRIREGDAVIAVSFSPYANETLNVVTATAR
                     PGVKHIAITDSQLSPLLSFSDVSFIIKEAQVSGFRSQCATMTLAQTIAVSLALKKA"
     gene            complement(1305..2645)
                     /locus_tag="PXD81_001310"
     CDS             complement(1305..2645)
                     /locus_tag="PXD81_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL5537332.1"
                     /translation="MSVSQRFIDFMNAHVAPVARRMENQPHISAIRDGFIVVLPFLIV
                     GSFIMILLIPPFDENTQNAFGQAWWRFANWASPYGWNFFQMSFNAISLFTSASIAYNL
                     AKAYKREPLPAAFLSVMAFLLVAAPVKDGMMDIKFFGGIGLFSAIFIAIYSVEMTRLL
                     EFLKIKIRLPKEVPHAVAESLNIVIPILAILLTLYPFAIWVESATGRNIPQLIMDFMA
                     PLIAVSDSLGAICLFVIATHLLWFLGINGSLVLMQLWTPFLLQNMAANLAAFQAGEPL
                     PFIITNSFWDFYIVHGASGGVIALAFLLVRSKSAHLRSIGKIGLVPSFFSIGEPIVYG
                     VPMVVNPLFFIPLIFAPLANAVIAYLILDFNLIHRVYLMAPWTTPAPIGAYLVSAGDI
                     WAPILSIALIILDIVIYYPFFKMYEKVCVEKEKAQNLDEKAQQELLEKARMAAQ"
     gene            complement(2648..3988)
                     /locus_tag="PXD81_001311"
     CDS             complement(2648..3988)
                     /locus_tag="PXD81_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HDL5537333.1"
                     /translation="MSCNTSPKRFHASWESIKQHQVPEWYEDAKFGIFIHWGIYSVPA
                     FAPPTCQLGEIDIDEQWFCNNPYAEWYFNSINVKKGPTYEHHIKTYGESFAYNDFLPL
                     WKAEKWQPAEWAKLFAKAGAKYVVLTTKHHDGFCLYPSKYTDFNCMQAGPKRDLMGEL
                     TENVREQGLKMGAYYSGIIDWTYSSAPIFTESQNFSNACPTYEYADYAYKQVVELIDR
                     YKPDVLWNDIGWPKVGEHMLPHLFAHYYNSVQEGVVDDRWNKLWCDFTSKEYKHGVAS
                     RDKKWEMCRGMGLSFGYNQVEDESHLISVKDLISLLVETVADNGNLLLNIGPKADGTI
                     PQEQVERLLALGSWLEINGEGIYKSRCSQHQTEQIGDVTYRYTKVGADLYLYIDGLTE
                     AQISLPLSVYGELQALNTELKFTIENTAQGRVLHIQNYQPDWYVLGFKIADGEK"
     gene            complement(4278..6194)
                     /gene="iolC"
                     /locus_tag="PXD81_001312"
     CDS             complement(4278..6194)
                     /gene="iolC"
                     /locus_tag="PXD81_001312"
                     /EC_number="2.7.1.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-2-deoxygluconokinase"
                     /protein_id="HDL5537334.1"
                     /translation="MATVNHQDRPLDLICLGRVAVDFYGQQIGSRLEEMTTFSKYLGG
                     SSGNVAYGTAVQGLRSSMLARVGDEHMGRFLREELQSVGVDTSHLITDKERLTALVIL
                     GIKDKETFPLIFYRDNCADMAITKDDFSEAYIASSRCLAITGTHLSNPKTREAVLTAL
                     EYAGRNEVKRALDIDYRPVLWGLTSLGDGETRYIDSAEVTKSLQEVLHHFDLIVGTEE
                     EFHIAGGSTDTLTALKNVRKVSNAVLVCKRGALGCSVFEGELPNDLDGGLNVYGVRVE
                     VLNVLGAGDAFMSGLLRGYLNNEGWEQACHYANACGALVVSRHGCSPAMPTKRELDDY
                     LTRAESVPRPDLDERLNHLHRVTTRKKQWENLCIFAFDHRKQLVDMANKVGADLARIP
                     KLKQLLLKAMEQTATQSGLPQNQAGILADTTFGQDVLNEITGKDYWIARPIEEPSSRP
                     LELEYGDLGSQLASFPRDHVIKCLAFYHPNDESGLKARQDRKLKEVYQACCRTGHELL
                     LEIILPADMPQSEAFYNQAIAHFYQLGIKPEWWKLPDVSRAQWQAISATIEANDPDCR
                     GILILGLDAPLEVFKDTFEQAKGCEKVKGFAVGRTIFGEPSEKWLAGELDDNTLISAV
                     SEKYRTLIDLWQQR"
     gene            6462..8396
                     /gene="iolD"
                     /locus_tag="PXD81_001313"
     CDS             6462..8396
                     /gene="iolD"
                     /locus_tag="PXD81_001313"
                     /EC_number="3.7.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015433647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3D-(3,5/4)-trihydroxycyclohexane-1,2-dione
                     acylhydrolase (decyclizing)"
                     /protein_id="HDL5537335.1"
                     /translation="MQTTRLTVAQALVQFLDNQYLDVDGKEIKFVHGVAAIFGHGNVL
                     GIGQALAQNAGGLTLYQGRNEQGMAHLAMGFAKQNLRQRIIACTSSVGPGAANMVTAA
                     ATATANRIPLLLLPGDVFATRQPDPVLQQIEQPYDLSISTNDAFRAVSKYWDRVQRPE
                     QLMSACINAFRVLTDPAETGAVTIALPQDVQAESFDFPNSFLQKRVHRLERTPPTTAM
                     IKQAVTLIQTKKKPLIVCGGGVRYSQAGQILQKFAKQFGIPFAETQAGKSAVVSDHAL
                     NVGGVGETGCLAANRLAKEADLVIGIGTRYTDFTTSSKWIFQNPNVQFLNINVARFDA
                     YKLDGVQLVADAKTALEALSQAIPTDYQAQWENEISQAKADFADEIQRLRAVQYHPDF
                     TPEINDNLDHKAVHNEFIQLTGSQLTQTQVLGIVNETIDNNAIIVAAAGSLPGDLQRT
                     WLSKGEETYHLEYGYSCMGYEVSAAVGVKIARPEQEVYALLGDGSYLMAHTELVTSIQ
                     EGKKINVVLFDNMANGCINNLQIGNGMDSFGTEFRFRNGETNQLNGGLVPIDFAKNAE
                     AYGCKTYRVTNEAELRAALDDAKKQTVSTLIDIKVLPKTMAHGYGCWWHVGVAEVSES
                     ERIQQARQRSEEQLAKARKY"
     gene            8523..9419
                     /gene="iolE"
                     /locus_tag="PXD81_001314"
     CDS             8523..9419
                     /gene="iolE"
                     /locus_tag="PXD81_001314"
                     /EC_number="4.2.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011817366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="myo-inosose-2 dehydratase"
                     /protein_id="HDL5537336.1"
                     /translation="MKAENIQLGIAPIGWTNDDLPELGAENTFEQCVSEMALAGYTGC
                     EVGNKYPRDVAVLKHKLDVRGIQICNAWFSTFFVDGKREETIAEFIKHRDFLHAMGAK
                     VIGCSEQSRSIQGTTKSVFKEKPIFNDEDWKRMAEGYNELAKLAAEKGMKVCLHHHMG
                     TGIQTPEEVDRYMAEVNDDVYLLFDSGHLYYSEGCQKAMLGVLEKYIDRIIHVHLKDV
                     RDEVMAEVKAKDLSFLEGVKKGTFTVPGDGVIDFRPIFDILEKHDYKGWMVVEAEQDP
                     ALANPFEYALKGRKYIRETAGV"
     gene            9421..10431
                     /gene="iolG"
                     /locus_tag="PXD81_001315"
     CDS             9421..10431
                     /gene="iolG"
                     /locus_tag="PXD81_001315"
                     /EC_number="1.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005537901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol 2-dehydrogenase"
                     /protein_id="HDL5537337.1"
                     /translation="MLKVGIIGAGRIGRVHSESISKYVKGAEIKAISDVKITEELTAW
                     AKSMGIPEVYDDYKKILQDPEIDAVLVCSSTNTHAPISIEAAQAGKHVFCEKPVDADP
                     AKIREVLSAVEKAGVKFQVGFNRRFDHNFKAIKDRVAAGDIGEPHVIRVTSRDPEAPP
                     IEYVKVSGGMFFDMTIHDFDMIRYLSGSEVEEVFAVGTVLVNPEIGKAGDIDTAVITL
                     KLKNGAIGVIDNSRKAAYGYDQRAEVFGSKGAIHITNDTGSTAVFSGENGVVAEKPKY
                     FFLERYMQSFADEISCFVDSVVNDKPTLVNGNDGLQPVLIALAAKKSLEENRPVKLSE
                     VA"
BASE COUNT         3051 a         2327 c         2375 g         2872 t
ORIGIN      
        1 aaaatgttta aaatatcata ataagagttt taaatataaa taggtatgga ggaaatatgt
       61 ctgataaatc tatcattcta gaaattatta atgctttagg aaacaaagag aatatcaagc
      121 aagttgaagc ttgtttaacc cgtttacgtg tggtgttaca taacaacggt ttattaaata
      181 agtcggcact gaaaaagctt ggggcggttg atgtcattaa agtcggtgat acgcaacaaa
      241 ttatttttgg tgcaaaagcc gggcagtatc gtgatgaaat taacgcttta atcggttaat
      301 agacaaagac ctgtatttac gcaggtcttt cactttctta atctaggctt ttttcaacgc
      361 cagtgaaacg gcaatcgttt gtgcgagcgt cattgtggca cactgggatc gaaaaccact
      421 cacctgtgct tctttaataa taaacgacac atcgctaaat gaaaggagtg ggctaagttg
      481 gctatcagtg atcgcaatat gttttacccc cggtctggca gttgctgtca ccacgttgag
      541 tgtttcattc gcatacgggg aaaagctcac ggcaatcacc gcatcacctt cacggattcg
      601 gcttaattgc tcgtcaaaca taccgcccga accatcgatc ataaagacat catagtccaa
      661 atggtgcaac gcatagttta ggtagttcgc aatgctgaat gagcgtttta aacccacaat
      721 aaaaatgcgt ttggcattgt gtaaaatatc taccgtttgt gctaactgct cttcgctgac
      781 ttgtgtcgat aactgttgca gtgcctgcgt attaccctgt acgaacacat ctaaaatatg
      841 attgagctca ctgttttgct catcaagcgg ttcaatttga cgaaaaagtt gcacacgttc
      901 ggtgtaacgt gatgtgcctt ccatcatatc ttggcgataa agctgtttaa tttcattaaa
      961 accgctaaat ccaaacgcat tcgcaaaacg gattaaggtt gaagggggaa catcagctcg
     1021 ttcagcaatg gtagaaaccg tatcaaacac cacgctgttg ccattatcca gcacatactg
     1081 tgccacttgt tttaaacgtt tacttagtcc gtcatactct tttcggattt gttcttgaaa
     1141 atgtgctaat tgagtaggtc cattcatttc aaattatctc tgcttgaatt ttttatttca
     1201 ttatacgctt ttcttgttta caagcggata aattttgtca attttttgca aaaataaggg
     1261 ctaatctttc gattagcccc tctcaattac gctagaaata agtgttattg tgctgccatt
     1321 cgtgcttttt ctagcaactc ctgttgtgct ttttcatcta gattttgtgc tttttctttc
     1381 tcgacgcaga ctttttcata cattttgaag aatggatagt agatcacaat atccaaaata
     1441 atcagtgcaa tacttaaaat tggtgcccaa atatcaccgg cagaaaccag atatgcccca
     1501 atcggtgccg gtgttgtcca cggtgccatc aagtaaacac ggtgaataag gttaaaatct
     1561 aaaatcaggt aggcaataac cgcattcgct agtggtgcga aaatcagcgg gataaagaac
     1621 agcggattaa ccaccattgg cacaccatac acaattggtt caccgataga gaagaaggac
     1681 ggaaccaagc cgattttacc gatagagcgt aagtgtgccg atttactgcg taccagcagg
     1741 aatgccaagg ctattacacc accacttgcc ccgtgtacga tatagaaatc ccagaaggaa
     1801 ttggtgatga taaaggggag cggttcacca gcttggaatg ctgctaagtt tgccgccata
     1861 ttttgcagta ggaatggtgt ccaaagttgc attagtacca atgagccgtt aatgcctaag
     1921 aaccagagca aatgggtagc aataacgaac aagcaaattg cccctagcga gtcactcaca
     1981 gcaatcaatg gcgccataaa gtccataatt agttgtggaa tattacgacc tgtggcactt
     2041 tccacccaaa tggcaaacgg atagagagtc agtaaaatcg ctaaaattgg aatcacgata
     2101 ttcagagatt ctgctacagc gtgcggcact tctttcggta agcggatttt aatttttaag
     2161 aactccaaca agcgggtcat ttctaccgaa taaatggcaa taaaaatcgc agagaatagc
     2221 ccaataccgc caaagaattt aatatccatc attccgtctt tgaccggtgc agccactaac
     2281 aaaaatgcca ttaccgagag aaatgccgct ggcaggggct ctcttttata ggctttggca
     2341 aggttgtagg cgatactggc agaggtaaat aatgaaatcg cattaaacga catttggaag
     2401 aaattccaac cgtaagggct tgcccaattc gcaaaacgcc accaagcttg cccgaaagca
     2461 ttttgggtat tttcatcaaa tggcggaatt agcaaaatca taataaagct gccgacaatt
     2521 aagaatggta acactacaat aaaaccgtct cgaatagcgg aaatatgcgg ttgattttcc
     2581 attcgacgtg ctactggagc aacgtgggca ttcataaagt caataaaacg ttgtgaaacg
     2641 gacataccta tttctcccca tctgcaattt taaagcctaa tacataccaa tccggctggt
     2701 agttttgaat atgtaacact ctgccttgtg cggtgttttc aatggtaaat ttcagctcgg
     2761 tattgagtgc ttgtagctca ccataaactg acagtggcaa gctgatttgg gcttcggtta
     2821 agccatcaat gtaaagatag agatccgccc caactttggt ataacgatag gttacatcac
     2881 cgatttgctc ggtttggtgt tggctacaac gggatttata gattccctct ccatttattt
     2941 ccagccagct ccccagtgct aataagcgtt caacttgctc ttgcggaata gtgccatctg
     3001 ctttcgggcc gatattcagc aataaattgc cgttgtcggc tacggtttct accaataggg
     3061 aaatcaagtc tttcaccgaa atcaaatggc tttcgtcttc tacctggtta tagccgaacg
     3121 ataagcccat accacggcac atttcccatt tcttatcacg ggaagccacg ccgtgtttgt
     3181 attctttgga ggtgaaatcg caccatagtt tgttccagcg gtcgtccact acgccctctt
     3241 gaacgctgtt gtaataatga gcaaataggt gtggtaacat atgctcgccc acttttggcc
     3301 agccaatatc attccacaat acatctggtt tatagcggtc gatcagctct accacttgtt
     3361 tataggcgta gtcagcgtat tcgtaagtcg ggcaagcgtt tgagaagttt tgactttcgg
     3421 tgaaaatcgg ggcagaggaa taagtccaat cgataatgcc cgagtagtaa gcgcccattt
     3481 ttaagccttg ctcccgcaca ttttcagtca gctcgcccat taaatcccgc ttcggtccgg
     3541 cttgcataca gttgaaatcg gtatatttac ttggatagag acagaaacca tcgtgatgtt
     3601 tggtggttaa taccacatat ttggcgccag ctttggcaaa cagtttcgcc cattctgctg
     3661 gttgccattt ttccgctttc catagtggca ggaagtcgtt ataggcaaag gattcgccat
     3721 aagttttgat atgatgttcg taggtcgggc cttttttgac attgatggaa ttaaaatacc
     3781 attctgcata agggttatta cagaaccact gttcatcaat atcaatttca cctaactgac
     3841 aggtcggtgg ggcaaaggcg ggtacggagt aaatccccca gtgaataaaa atcccaaact
     3901 tagcatcttc ataccactct ggtacttggt gctgtttgat cgactcccaa ctggcgtgga
     3961 aacgttttgg tgaagtgttg caactcatac acagactcct attgaatcta aatagaacta
     4021 aactgaaata cattttccat tttgataaaa ataaaacaat ttgtttcgat aaaaaggggg
     4081 ggggtttatg tatgaaatgt gaacttcatc acacttattt tgtgataagc agattgagga
     4141 atgcccgata ttcgcttttt taggaaaatt cgggcatttt tacggctagc aagcggtctg
     4201 tttagccgga ttttttgcaa aacaaaaccc cttgcttaat atcataagct aaggggtttt
     4261 cacttaaagc aatatgatta acgttgttgc cagagatcga ttaaagtgcg atatttctcc
     4321 gataccgcag aaatcaaggt attgtcgtcc aattcaccgg caagccattt ttcggacggt
     4381 tcaccgaaaa tggtgcggcc gactgcaaag cctttcactt tctcacagcc ttttgcctgt
     4441 tcaaaggtgt ctttaaacac ctctaacggg gcatctaaac ctaaaattaa aataccacga
     4501 cagtccggat cgtttgcctc aatggtggcg gaaatcgctt gccattgtgc gcgagaaaca
     4561 tccggcaatt tccaccattc cggcttaatg ccaagttggt agaaatgtgc tatcgcttgg
     4621 ttatagaaag cctcactctg tggcatatca gcaggcagaa taatttccaa caatagctcg
     4681 tgaccggtac ggcaacaggc ttgatacacc tcttttaact tgcgatcttg gcgggctttt
     4741 aaaccgcttt catcattcgg gtggtaaaat gccaagcatt tgatgacgtg atcgcgtggg
     4801 aatgatgcta gctgagagcc taaatctcca tactctaatt ccagcgggcg ggaagacggc
     4861 tcttcaatcg ggcgggcaat ccagtaatct ttgccggtaa tctcattaag tacatcttgc
     4921 ccgaaagtgg tatcggctag gatccctgct tgattttgtg gtaagccgga ttgagttgcg
     4981 gtttgttcca tcgcttttaa tagcagctgt ttcagtttcg gtatgcgagc aagatctgca
     5041 ccgactttat ttgccatatc caccaactgt ttgcggtgat caaaagcaaa aatacagaga
     5101 ttttcccact gttttttgcg ggtggtgaca cggtgtaagt ggtttaaacg ctcatctaaa
     5161 tccggacgtg gtacactctc tgcacgggtt aaataatcgt caagttcacg tttggtcggc
     5221 attgccggtg aacagccgtg acgagatacc actaacgctc cgcacgcatt ggcatagtgg
     5281 cacgcttgtt cccaaccttc gttatttaag tagccacgca gtaagcccga cataaaagca
     5341 tcgcctgcac ccagcacatt cagcacttct acacgcacgc cgtacacatt caagccaccg
     5401 tctaaatcat tcggcagctc gccttcaaat actgaacacc ctaatgcccc acgtttgcac
     5461 actaatacgg cattagagac tttacgcaca tttttgagtg cagtgagagt atcggtcgaa
     5521 ccgcctgcga tatgaaattc ttcctctgtg ccgacaatca gatcgaaatg gtgcaatact
     5581 tcttgtagcg atttggtgac ttcggcactg tctatataac gggtttcgcc atcaccgagt
     5641 gaggttaatc cccataacac agggcgataa tcaatatcta acgctcgttt tacctcattt
     5701 ctacccgcat attcgagtgc ggtcagcaca gcttcacggg ttttagggtt tgacaggtgc
     5761 gtgccggtaa ttgctaagca acggctggaa gcaatatagg cttcgctaaa atcgtctttg
     5821 gtgattgcca tatccgcaca gttatcacga tagaaaatca gcggaaaagt ctctttatct
     5881 ttaatgccga gaataaccag tgcagttaag cgttctttat cggtaattaa atggctggtg
     5941 tccacgccta cgctttgtag ctcttcacgt agaaaacgcc ccatatgttc gtcaccgacc
     6001 cttgccagca ttgaagaacg taaaccttgc accgccgtac cgtaagccac attgcctgac
     6061 gagccgccga ggtattttga aaatgtggtc atctcttcca aacgggaacc gatttgttgc
     6121 ccataaaagt ccaccgccac acgccctaaa cagataagat ccaatggacg atcttgatga
     6181 ttaactgttg ccataaccgc tccttacaac aagtaaattt gaatacacac tatacatcat
     6241 acaaaaatga aatttttatt tcaaatttat tctatttaaa ttatcatttc tgtgatctag
     6301 ctctcacttt gaaaaataag gcaataactt cgcagaaatg agaactggat cacatctttt
     6361 tttcgatttc cttgctatta taaatgaaat aaatttttca tttgtaaaaa tcagtagaaa
     6421 accgaccgct tgcaaaagcg atgtaagcca gaggaggctt tatgcagact actcgtttaa
     6481 cggttgcaca ggcgttagtg caatttttgg ataatcaata tttagacgtg gacggcaaag
     6541 agattaaatt cgttcacggt gttgccgcta ttttcggcca cggcaatgtg ctgggaatag
     6601 gtcaggcgct tgctcaaaat gccggtggtt taacgctcta ccaaggcaga aatgagcaag
     6661 gtatggcaca tttggcgatg ggctttgcta agcaaaatct gcgtcaaaga attattgcct
     6721 gtacttcctc ggtcgggccg ggggcggcaa atatggttac ggctgcggca acggcaacag
     6781 cgaaccgtat tcctcttctt ttattaccgg gcgatgtgtt tgcgacccgc cagccggatc
     6841 cggtactgca acaaatcgaa caaccttacg atttaagcat tagcactaac gatgcgttcc
     6901 gtgccgtcag taaatattgg gatagagtgc aacgccctga gcagttaatg agtgcttgta
     6961 tcaatgcgtt tcgtgtgctg accgaccctg ctgaaaccgg tgctgtcacc attgctctgc
     7021 cgcaagatgt acaagcggag agttttgatt tcccgaacag cttcctgcaa aaacgagtgc
     7081 atcgcttaga gcgtacaccg ccgacaacag cgatgattaa gcaagcggta acattaattc
     7141 aaaccaagaa aaaaccgctg attgtctgcg gtggcggtgt tcgctactcg caagcgggtc
     7201 aaattctgca aaaatttgct aaacagttcg gcattccatt tgctgaaacc caagcgggta
     7261 aaagtgcggt ggtgtccgat cacgcattga atgtaggcgg agtgggagaa accggttgtt
     7321 tagcggcaaa ccgtctggca aaagaagcgg atttagtgat tggcattggt acacgctaca
     7381 ccgacttcac gacttcttcc aaatggattt tccaaaaccc aaacgtgcag tttttgaaca
     7441 ttaatgtggc acgttttgat gcgtacaaat tagacggcgt gcaactggtt gccgatgcaa
     7501 aaactgcatt agaggcgtta agccaagcca ttcctactga ttatcaagct caatgggaga
     7561 atgaaatttc acaggcgaaa gcggattttg ctgatgaaat tcaacgcctg cgcgctgtgc
     7621 aatatcaccc tgatttcacc cctgaaatta acgataatct tgatcataaa gcggttcaca
     7681 atgaatttat tcagctcacc ggttcccagc tcacccaaac ccaagtgctg ggtattgtga
     7741 atgaaaccat tgataacaat gcgattattg ttgccgccgc cggaagtttg ccgggtgatt
     7801 tacaacgcac ttggttaagt aaaggtgaag agacttacca ccttgaatat ggctactcct
     7861 gtatgggcta tgaagtgagt gcggcagtcg gcgtgaaaat tgcccgcccc gaacaagagg
     7921 tctatgcctt gctgggggac ggctcttact taatggcaca caccgaatta gtgacctcaa
     7981 ttcaagaggg caaaaaaatc aacgtggtgc tgttcgacaa tatggcaaac ggctgtatca
     8041 acaacctgca aatcggcaac ggtatggaca gtttcggtac ggaattccgc ttccgcaatg
     8101 gcgaaactaa ccaactcaac ggcggtttag tgccgattga ttttgccaaa aacgccgaag
     8161 catacggctg caaaacctat cgggtaacta acgaagccga attgcgtgcc gcccttgatg
     8221 atgcgaaaaa acagaccgtt tctaccttaa tcgatattaa agtgttgccg aaaacaatgg
     8281 ctcacggcta tggctgctgg tggcacgttg gtgtcgcgga agtgtcagag agcgagcgta
     8341 ttcaacaagc ccgccaacgc tccgaagagc agttagctaa ggctcggaag tattagttaa
     8401 aaaaacaact ccccactttg agctacgagg ggagaatatc aagcggtcaa atttgcaaaa
     8461 tttttgataa aaataaccgc ttgcatccaa atttatctct ataaatcaaa cgaggtttct
     8521 ctatgaaagc tgaaaacatt caattaggca ttgccccaat cggttggacg aacgatgacc
     8581 tgccggaact gggagcggaa aacactttcg agcagtgtgt gagtgagatg gcacttgccg
     8641 gctacacggg ctgtgaagtg ggtaataaat atccgcgtga tgttgccgtg ttaaaacaca
     8701 aactggacgt gcgtggcatt caaatctgta atgcgtggtt cagcaccttc tttgtggacg
     8761 gtaaacggga agagaccatt gccgagttca tcaaacatcg tgatttcttg catgcaatgg
     8821 gggcaaaagt gatcggctgt tccgaacaga gccgtagtat tcaaggcaca accaaatctg
     8881 tgttcaaaga aaaaccgatt ttcaacgatg aagattggaa acgtatggca gaaggctata
     8941 acgaattggc gaaacttgcc gccgaaaaag gaatgaaagt ctgcctgcat caccatatgg
     9001 gaaccggtat tcaaacaccc gaagaggtcg atcgctatat ggcagaagtg aatgacgatg
     9061 tctatctgtt gttcgattca gggcatttgt actattccga aggctgccaa aaagcgatgc
     9121 tcggcgtact ggaaaaatat atcgaccgta ttatccacgt tcacttaaaa gatgtgcgtg
     9181 atgaagtcat ggcggaagta aaagcaaaag atttaagctt tttagaaggc gtgaaaaaag
     9241 gcacctttac tgtaccgggc gatggtgtca ttgatttccg tccgattttc gatattttag
     9301 aaaaacacga ctacaaaggc tggatggttg tagaagccga acaagatccg gcacttgcca
     9361 acccgtttga atacgccctc aaaggcagaa aatatatccg tgaaaccgca ggagtttaat
     9421 atgttaaaag taggtattat tggtgcaggc cgtattggac gtgtgcattc tgaaagtatt
     9481 agcaaatatg tcaaaggtgc tgaaatcaaa gcaatctctg atgtaaaaat taccgaagaa
     9541 ttgaccgctt gggcaaaatc gatgggtatt ccggaagttt atgatgacta taaaaaaatc
     9601 ctgcaagatc cggaaattga tgctgtattg gtctgctctt ccaccaacac tcacgctccg
     9661 atttcgattg aggcagcaca ggctggcaaa cacgttttct gtgaaaaacc ggtagatgca
     9721 gacccagcta aaatccgtga agtattgagt gcagtcgaaa aagccggtgt aaaattccaa
     9781 gtgggcttta accgccgctt cgaccacaac ttcaaggcga ttaaagaccg tgtggcggca
     9841 ggcgacatcg gcgaaccgca cgttatccga gtaacctctc gtgatccgga agccccgcca
     9901 atcgaatatg tgaaagtctc cggcggaatg ttctttgata tgaccattca cgacttcgat
     9961 atgattcgtt atttatccgg cagcgaagtg gaagaagtgt ttgcagtcgg caccgtatta
    10021 gtaaaccctg aaatcggcaa agcgggcgat attgatactg ccgttatcac gttgaaactg
    10081 aaaaacggag caatcggcgt aatcgacaac agtcgcaaag ctgcctacgg ctacgaccaa
    10141 cgtgcggaag tgttcggctc gaaaggggca atccatatca ctaatgatac cggctcaact
    10201 gccgtattct caggtgaaaa cggcgtggtg gcggaaaaac caaaatactt cttcttagaa
    10261 cgttatatgc aatctttcgc cgatgaaatc agctgtttcg tagattctgt ggtaaacgac
    10321 aaaccaacgt tagtaaacgg caatgacggc ttacaaccgg tacttattgc cttagctgcg
    10381 aaaaaatcac tggaagaaaa ccgaccagtg aaattgagtg aagtggcgta attgatcata
    10441 aatacaagcg gtcggtttta ctcgctacac tcgcacttcg tgccatcggc atagccgatg
    10501 ttcaaacgca agcattttgc agaaaaattt gtaaaactga ccgtttgagt ttttatttat
    10561 tatcttttgg gaaataaata taatcgtgct caatttttat tgattgttta aataaataga
    10621 aggct
//
LOCUS       DANLYI010000072        10575 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.072, whole genome shotgun sequence.
ACCESSION   DANLYI010000072 DANLYI010000000
VERSION     DANLYI010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 10575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10575
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.072"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            16..750
                     /locus_tag="PXD81_001316"
     CDS             16..750
                     /locus_tag="PXD81_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HDL5537338.1"
                     /translation="MARSSYFARLAEWTLLEHLLVKQPENISVCSFGQSFGTSGRLDA
                     EYYQPKFASLFAQLANFKTMKLGEIVNLQKSVEPGSEAYQTEGIPFVRVSDLSKFGIS
                     QTDKYLHPKDFGNVVRPKKDSILLTKDGTVGIAYRVPQDLNVITSGAIVHLELKTDEV
                     LPDYLALALNSPAVQLQAERDAGGSIIQHWKPSEILDVVIPVLPKNIQQTISDKVQQS
                     FALRVESEVLLEKAKILVEQEIENMR"
     gene            753..1388
                     /locus_tag="PXD81_001317"
     CDS             753..1388
                     /locus_tag="PXD81_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5537339.1"
                     /translation="MPNHNQDLKKEWFNKARIDYHSPFLMLWLSCNSWYNFHYSLGKD
                     RTHIDRIKSDTSNQNKLYKEFERIFTSGKIKEKTNLWNNIEQLHFALVQAELKYSGSN
                     IPSEYSKFNLENVLIDFPNKTNSVAYQNLVIHNAKTRAGKLKTQYANAHDLGNLVLVE
                     DIQKIFSGLLEIIYQVRCHLVHGSLSPTPENHEVVKYCYLILWDCLKGFCD"
     gene            complement(1656..2393)
                     /locus_tag="PXD81_001318"
     CDS             complement(1656..2393)
                     /locus_tag="PXD81_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL5537340.1"
                     /translation="MIKIENLTLAHGIQHLSCYLPKGKLIGIMGANGAGKSSLLKAIA
                     GILRPTSGEIWLENCKLHALSAVERSRQFAYLAQQLNVAWQLSVYDVIALGLPAPLPP
                     EQERQKVRSVAEQLAISHLLDNTYQQLSGGEQPRVQLARCLMKQAPILLADEPIAALD
                     PYYQIDIMQQLKALTPTQTCVVVIHHLPLAYRFCDEIILLKNGNLLVSGNTQAVLNAE
                     NIATAFGVKAEMDWEKREMWGIEKLEV"
     gene            complement(2390..3358)
                     /locus_tag="PXD81_001319"
     CDS             complement(2390..3358)
                     /locus_tag="PXD81_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HDL5537341.1"
                     /translation="MKKLNIVLGSLLLLIVSNAAFYQLSDFALLKNAEGVLSDLRWLV
                     LGEIRLPRIGLAVLTGASLALAGNAMQGVFKNPLASPGLLGSSSGATAVSVFLLYYFS
                     APMGWLLFGGVAGALGAFLLVWLISFRHGTTMMILSGVAVNVLLGAAVTLLLSNAESP
                     WALAELYRWLQGSLVWATAEAVCWALPLILLGIVCLYRERRYLDLLTFGEETAATMGI
                     NLQRSFFTVSIGVALLVGATIPQTGTIGFIGLIAPHLARIWLKKPPSQLYLTSALIGA
                     LLLLIADLAVQYVPFFARIYIGTLTAILGVPFLIWILFTQQWRLAQ"
     gene            3539..3874
                     /locus_tag="PXD81_001320"
     CDS             3539..3874
                     /locus_tag="PXD81_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDL5537342.1"
                     /translation="MYHIERTEIFDQWLENLKDPLAVVTIARRIERAESGNFGDTKYI
                     AEGISEMRLNVSKGYRLYYAQKGSIIYILLNGGHKGTQQADIDKAKMLWAEIKQHMAE
                     QEKKNERII"
     gene            3858..4181
                     /locus_tag="PXD81_001321"
     CDS             3858..4181
                     /locus_tag="PXD81_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative addiction module antidote protein"
                     /protein_id="HDL5537343.1"
                     /translation="MNELYDAPIPQATREKFGIQPFDAAEYLANDELIALYLAETLKD
                     GTDEEFIQALNTVARAKGMNELAKKAGIGRESLYQSLSSSKPRFETIRKIISALNLEL
                     SVVKA"
     gene            complement(4171..6057)
                     /locus_tag="PXD81_001322"
     CDS             complement(4171..6057)
                     /locus_tag="PXD81_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HDL5537344.1"
                     /translation="MTQLDSVTVYSAYATPINQDKTASSVTVLTEKDFAARNATYVSD
                     VLKTVPGVAIGQQGGRGTLTSLFLRGAESRHTAVVIDGVKVNPINIGNFDFGGLPISN
                     IERIEVLRGEQSALWGSSAMGGVVYITTKSGLYKEKPFNAEVDLGLGSNNTRDASATL
                     SGFHNGFYYALHGDSHRTKGISALSKNHFSYTTETGSEVKTGGASERDGFHRDNGSLR
                     LGYDLGNKGVEVLAAQSSQTVHIDGYNSDVSGEYSRTRNQTFKLGGYWGNEQELLKHQ
                     ANISQFNSKATHFGSNARYSNEKQLNANYQLDVNFDREGEVTQAVSLLTDYAKTRYTS
                     DKYLREKTLSEKSAALEYRLFTEQDHSFSISGRYTDNSQFKNSITGRISGAYRLSPNL
                     CSDRLLLELAEPQQIRAMSPYSQKPLMMLDKLNTDKPTVEPELTALLPYADSTILLNE
                     TFYPQLTARLKQLGFKLVALNDSPQTPEQLFTLILQLGELTQNQAKAEKLVERLRLQK
                     IPLKQPLAETLILSETGMIEPHFPQYQTLLDLLGLSPLKSDLTPQNFSLEKLLLAQPK
                     QLLFLTDNQSYNNQAELLKHPALQKIWQKMSQNPPLVLPMKYTYCFDHGVWQGIQLMH
                     KLTP"
     gene            6087..6887
                     /gene="metF"
                     /locus_tag="PXD81_001323"
     CDS             6087..6887
                     /gene="metF"
                     /locus_tag="PXD81_001323"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HDL5537345.1"
                     /translation="MQQKWRMLWDSIDRLSPLKPKFVSVTYGANSGERDRTHSIVKSI
                     QEKTGIISAPHLTGIDATPEELKVIAKDYWDSGIRRIVALRGDIPKGYDKALFYASDL
                     VALLKSVADFDISVAAYPEVHPEAKSAQADLLNLKRKIDAGANHVITQFFFDVESYLR
                     FRDRCVSIGIDCEIVPGILPVTNFKQLQKMAAVTNVKIPTWMGKMYEGLDDDQTTRNL
                     VAASIAMDMVKVLSREGVNDFHFYTLNRSELTYAMCHTLGVRPDLTTA"
     gene            complement(6936..8096)
                     /locus_tag="PXD81_001324"
     CDS             complement(6936..8096)
                     /locus_tag="PXD81_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5537346.1"
                     /translation="MKKSILLILSLFSASLFADVQLSPLPLPQIGKVSQFDLSEQGEL
                     VVINQQKQLWRVTQQAQKLADGVSTEIEPKAQYQRIALADQNGHFMLWTADKIYTSNI
                     PLAPKATMTALNFATIAVTKQNGQFKLARIETQGNQATITATAETEVLPDAHPLQIDF
                     DAETPNQGHIAVLAKPDDFTYQHGVLGDDVEAGEIQFLERHTLNPLAEKLTQQGRVFE
                     ANRWATFTENGKTKIVSVISGDGNGASTVVIGVKDGKLSIEAESEPLSTNRWQSPFIF
                     NRKLYAIQMPHLKGNLVEYSLKGNQLAERFITSGLSNHRYGDHETNLVATTETFALIP
                     QMGYQQVVVLDKTGKITELPTALPAGIKQTKADSNKAYLLLKNGEIWVAEEK"
     gene            complement(8156..9172)
                     /gene="galE"
                     /locus_tag="PXD81_001325"
     CDS             complement(8156..9172)
                     /gene="galE"
                     /locus_tag="PXD81_001325"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005598364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HDL5537347.1"
                     /translation="MAILVTGGAGYIGSHTLVELLNENREIVVLDNLSNSSEVSLERV
                     KQITGKSVKFYQGDILDRDILRKIFAENQIESVIHFAGLKAVGESVREPLRYYQNNVT
                     GSIVLVEEMLKANVNTIVFSSSATVYGDPQIIPIVESCPVGGTTNPYGTSKYMVERIL
                     EDTVKAFPQLSAVVLRYFNPVGAHESGLIGEDPNGIPNNLMPFISQVAVGKLPQLSVF
                     GGDYNTHDGTGVRDYIHVVDLALGHLKALDKHQNDAGFHVYNLGTGTGYSVLDMVKAF
                     EAANGITIPYKVVDRRPGDIAVCYSAPQKALEQLGWETERGLEQMMKDTWNWQKNNPN
                     GYKG"
     gene            complement(9260..9421)
                     /locus_tag="PXD81_001326"
     CDS             complement(9260..9421)
                     /locus_tag="PXD81_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HDL5537348.1"
                     /translation="MNETLLNNLVCPVSNEKLEWDKENNRLINPKLNIAYPIKNGIPE
                     LLPEAGVAL"
     gene            complement(9399..10400)
                     /gene="lpxK"
                     /locus_tag="PXD81_001327"
     CDS             complement(9399..10400)
                     /gene="lpxK"
                     /locus_tag="PXD81_001327"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HDL5537349.1"
                     /translation="MKIWQTKSLITWLLSPLSLLFWLISQARKRLYSLNILQSYRSPL
                     PMLVVGNISVGGNGKTPVVVWLVEQLQQRGVKVGVISRGYGGKAKTFPQLVTRESSAE
                     MVGDEPVLIVQRTNVPLAISPNRQQSIELLLNQFEPDIIITDDGLQHYALQRDMEWVV
                     VDGVRRFGNGFVLPAGGLRELPSRLNSVQAVICNGGTPQANEHLMTLEPSDAINLVTN
                     QRKPLSEFTQPCVAIAGIGHPPRFFTMLEKLGIPLAKTQGFADHQAFDKAQLAQFADS
                     QTPLLMTEKDAVKCHDFAQPNWWYVPVSATFSEKSTACLLAPILQKLGKEDERNTTQ"
     gene            complement(10402..>10575)
                     /locus_tag="PXD81_001328"
     CDS             complement(10402..>10575)
                     /locus_tag="PXD81_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HDL5537350.1"
                     /translation="NLTNSFISLGNGVEEIQRLQLIISHKDELLAHKEEIIENQKREL
                     ALLRDLVETLKNK"
BASE COUNT         2988 a         2234 c         2314 g         3039 t
ORIGIN      
        1 aagccttatg gtttattggc gagatcttct tattttgcac gtttagcaga atggacttta
       61 ttagaacatt tacttgtcaa acagcctgaa aatatttcgg tatgttcatt cggtcaatca
      121 ttcggtacaa gcggtcgttt agatgcagaa tattaccaac ctaaatttgc aagcttattt
      181 gcacaattag ccaatttcaa aacgatgaaa ttaggtgaga ttgttaattt acaaaaatcg
      241 gtagaaccgg gctcggaagc ttatcaaaca gagggaatac cctttgtgcg tgtatcggat
      301 ttatctaaat tcggcatcag ccaaacagat aaatacttac atccgaaaga ttttggcaat
      361 gttgtaagac ctaaaaaaga cagtatatta ctgactaaag acggcacagt cggcattgct
      421 taccgtgttc cgcaagattt gaatgtgatt acatcagggg caattgtgca tctagagctt
      481 aaaactgacg aagttttacc cgattatttg gcgttagcat tgaattctcc tgctgtacaa
      541 ttacaagcgg aaagagatgc gggaggctct attatccaac attggaaacc gtctgaaatt
      601 ttagatgtag tgattccggt attacctaaa aatatccagc aaaccattag cgacaaagta
      661 caacaaagtt ttgcattgag agtagaaagt gaagttttac tggaaaaagc taaaatactt
      721 gtagaacaag aaatagaaaa tatgaggtaa taatgcctaa tcataatcaa gatttgaaaa
      781 aagagtggtt taataaagca aggatagatt accactctcc ttttttaatg ttatggctca
      841 gttgtaatag ctggtataac ttccattatt ccttaggtaa ggatagaaca catattgata
      901 gaataaaatc ggataccagt aatcaaaaca aactttataa agagtttgaa agaatattca
      961 cctctggaaa aataaaagaa aaaactaatt tatggaataa tattgagcaa cttcattttg
     1021 cattagttca ggcagagtta aaatattcag gtagcaatat tccaagtgaa tattctaaat
     1081 ttaacttgga aaatgtgctt attgattttc ctaacaagac aaattctgta gcatatcaga
     1141 atctagttat tcacaacgca aaaacgcgtg caggaaaatt aaaaacacaa tatgcaaatg
     1201 ctcacgattt aggcaatttg gttttagtag aggatatcca aaaaatattt tccgggttat
     1261 tagaaataat ttatcaagtt cgttgccatt tggtacatgg tagcttaagt ccaacacctg
     1321 aaaaccacga agttgttaaa tattgctatt tgattttgtg ggattgttta aaaggatttt
     1381 gtgattagtt attgtcaata aagctacaag cggtcaattc tcccaaaact ttgcaaattt
     1441 ccccgcaaaa ctgaccgctt gttctcctct atccattcac ctcaaccaac ttttctctct
     1501 acccctactc aaaatcggtt aaacaatctg ttgaggtaaa tgttgtgtcg gaatgggatt
     1561 taatcttaat gaaactgaaa aaatcttctg ccaagcaaat ttccctaaaa ttggaagttc
     1621 atttccaatt tctcaaaaaa tcacccaaac cggaactaca cttccaattt ttcaatcccc
     1681 cacatttccc ttttctccca atccatttca gctttcaccc caaaagccgt tgcaatattt
     1741 tcggcattta agaccgcttg tgtgttgccg ctgacgagta aattgccgtt tttaagcaga
     1801 atgatttcat cacaaaaacg gtaggcaagg ggcaaatgat gaatcaccac cacgcaggtt
     1861 tgtgttggag ttagggcttt gagctgctgc ataatatcga tttggtagta ggggtcgagg
     1921 gcggcaatcg gttcatcggc aagcaagatc ggggcttgtt tcatcaaaca acgggcgagt
     1981 tgcactcggg gctgttcacc gccggagagc tgttggtaag tgttgtccag caggtgagaa
     2041 atcgccaatt gttcggcaac cgaacgcacc ttttgacgct cttgttcagg cggaagcggt
     2101 gcgggcaagc cgagtgcaat cacatcatac accgaaagtt gccacgccac gttgagctgt
     2161 tgggcgaggt aggcaaattg tcggctcctc tcaacggcgg aaagggcgtg cagtttgcaa
     2221 ttttccagcc aaatttcacc gcttgtcggg cgtagaatgc cggcaatcgc tttgagcaac
     2281 gaggatttcc ctgccccgtt tgcgcccata atgccgatca atttgccttt tggcagatag
     2341 caagagaggt gttgaatacc gtgggcaagg gtgagatttt caatcttgat cattgtgcca
     2401 atctccattg ctgggtgaat aaaatccaaa tcaaaaacgg tacgcccaaa attgccgtga
     2461 gcgtgccgat gtaaattcgg gcaaaaaacg gcacatattg caccgccaga tctgcaatca
     2521 gcagtaacaa cgccccgatt aaagcggagg taagataaag ctgggacggc ggttttttca
     2581 gccaaatacg ggcaagatga ggggctatca agccgataaa accgatggtg ccggtctgtg
     2641 gaatcgttgc tccgaccaac agcgccacgc caatgctaac ggtaaaaaaa ctgcgttgca
     2701 ggttgatgcc cattgttgct gccgtctctt cgccaaaggt gagcaggtcg agataacgcc
     2761 gttcccgata gaggcagaca atgccgagca agatcaacgg taacgcccaa catactgcct
     2821 ctgcggttgc ccagactaat gagccttgca accagcggta gagttccgcc aacgcccacg
     2881 ggctttcggc attggaaagc agtaaagtca ccgctgcccc gagcaacacg tttaccgcca
     2941 cgccacttaa aatcatcatt gttgtgccgt ggcgaaagct aatcagccac accagcaaaa
     3001 atgcccccag cgcccctgcc acaccgccaa acagtagcca gcccatcggg gctgaaaaat
     3061 agtagagcag aaatacgctc accgccgttg cccccgaact gctcccaagc aaaccggggc
     3121 tggcaagcgg atttttaaac acgccctgca tcgcattgcc ggcaagcgca aggctggcgc
     3181 ccgtcagcac cgccaagcca atacggggca gtcgaatttc acccagcacc agccagcgca
     3241 gatcgcttag cacgccttcc gcatttttaa gcaaggcaaa atcgctcagt tggtaaaagg
     3301 ctgcgttgct aacaatcagc aacaacaagc ttccaagcac aatgtttaat ttcttcattc
     3361 ttctttctct catcaaacgt gcaaattata ccatagcgcc aaagtcaagc tgaaaacgct
     3421 atttttgcga tcatcatcga aaaactagcg tttgccgaat gtaacccacg acaggctttg
     3481 ctatattcct tcaatcagcc taagttttgt atttaaaaca ttacagattg ggggtagaat
     3541 gtaccatatt gaacgcactg aaattttcga ccaatggctt gaaaatttaa aagatccact
     3601 tgctgtggtg accattgcaa gacgtattga acgtgccgaa agcggaaatt ttggcgatac
     3661 caaatatatc gcagaaggaa tttccgaaat gaggctaaac gtcagcaaag gttatcgcct
     3721 ttactatgcg caaaaaggta gcattattta tatactacta aatggcggac ataaaggcac
     3781 gcaacaagct gatattgata aagctaaaat gctatgggca gaaattaaac aacatatggc
     3841 agaacaggag aaaaagaatg aacgaattat atgacgcccc aattccacaa gctactcgtg
     3901 aaaaatttgg cattcaacct ttcgatgccg ctgaatacct cgccaatgac gagctaatcg
     3961 ccctttattt ggcggaaaca ttgaaagacg gcacggacga ggagtttatt caagcattga
     4021 ataccgtggc acgagcgaaa ggaatgaatg agttagcgaa aaaagcaggg atcgggcgtg
     4081 aaagtttgta tcaaagcctt tccagcagta agccccgttt tgagactatc cgcaaaatta
     4141 ttagcgcatt aaatttggag ttgtcggtcg ttaaggcgta agtttgtgca ttaattgtat
     4201 cccctgccaa accccgtggt cgaaacagta ggtgtatttc atcggtagga caagcggtgg
     4261 attttggctc attttttgcc atattttttg taatgctggg tgtttgagca gttcggcttg
     4321 gttgttatag ctttggttat cggtgaggaa caataactgt ttgggttgcg ctaacagcag
     4381 tttttctagc gagaaattct gtggcgtgag gtcgcttttt aatggtgaca gaccgagaag
     4441 atccaacaac gtttgatatt gcggaaaatg cggttcgatc atacccgttt cgcttagaat
     4501 taaagtttca gcaagcggtt gttttagcgg aattttttgc aaacgtagcc gttccaccag
     4561 cttttccgct ttggcttgat tttgggttag ctcgcccagt tgtaaaatca gtgtgaaaag
     4621 ctgttcgggt gtttgcggac tgtcgtttaa tgcgactaac ttaaagccaa gctgtttcag
     4681 ccgtgcggtg agttgcggat agaaggtttc attcagcaaa atggtgctgt cggcataggg
     4741 caacagggcg gtgagttcag gctcaacggt gggcttgtcc gtgttcagtt tatccaacat
     4801 catcaacggc ttttgggaat agggcgacat cgcccgaatt tgctgtggtt cggcaagttc
     4861 cagcaacaga cggtcgctac acaaatttgg cgataaacgg taagctcctg aaatacgccc
     4921 agtgatagaa tttttaaact gtgaattatc ggtgtaacgg ccactaatag aaaaactgtg
     4981 atcttgttcg gtaaataagc gatattctag tgccgcactt ttttcggata aggttttttc
     5041 acgcaaatat ttatcagagg tatagcgtgt ttttgcataa tctgtcagta aactgaccgc
     5101 ttgtgtcact tctccttcac ggtcaaagtt tacatctaat tgataattcg cattaagttg
     5161 tttttcgttg ctgtagcgag catttgaacc gaaatgtgtt gctttactat tgaattggct
     5221 gatatttgct tggtgtttca gcagctcttg ttcatttccc caataaccgc ctaatttaaa
     5281 agtttgattg cgagtacggg aatattcacc gctaacgtct gaattatagc catcaatatg
     5341 aacggtttgg gaagattgag cggcaagaac ttctacccct ttgttaccga ggtcataacc
     5401 taaacgtaaa gagccgttat cacgatgaaa gccatctcgt tcgcttgcac caccggtttt
     5461 cacttctgag cctgtttctg tggtatagct gaaatggttt ttactcagtg cggaaatgcc
     5521 tttggtacga tggctatcac cgtgtaaggc atagtagaaa ccgttgtgaa atccggaaag
     5581 tgttgcgctg gcatcacggg tattatttga gcctaaacca agatcgacct ccgcattgaa
     5641 tggtttctct ttgtataaac cgcttttagt ggtgatataa actacaccac ccatagcact
     5701 gcttccccaa agggcggatt gctcgccacg caacacttcg atacgttcga tattgctaat
     5761 cggtaagcca ccaaaatcga aatttccaat attaatcgga ttgactttta caccgtcaat
     5821 cacaaccgca gtatggcgag attctgcccc tcgtaaaaat aagctagtaa gagtaccacg
     5881 cccaccttgt tgcccaatcg ccacgcccgg cacagtcttt aacacatcac tcacataagt
     5941 cgcattgcgt gcggcaaaat ctttttcggt aagaaccgtc acgctggaag cggttttgtc
     6001 ttggttgatt ggggttgcat aggcggaata gactgttacg gaatcaagct gagtcatctc
     6061 attttgtgca aaagctgtac ttgctagtgc aacagaaatg gcgaatgctg tgggattcta
     6121 ttgatcgcct aagtccttta aaaccgaaat ttgtttcggt aacttatgga gcaaattctg
     6181 gtgagcgaga tcgtacacac agtattgtga aatcaatcca agaaaaaaca gggatcattt
     6241 cagctccgca cttaacaggc attgatgcaa caccggaaga attaaaggta attgccaaag
     6301 attattggga tagtggtatt cgccgaattg ttgctttgcg tggcgatata ccgaaaggtt
     6361 atgataaagc actattttac gcctcagatt tagtcgcttt attaaaatca gtagcagatt
     6421 tcgatatttc cgtggctgcc taccctgaag ttcaccctga agcaaaatca gcacaggcag
     6481 atttacttaa cttgaaacgt aaaatcgatg cgggtgcaaa ccacgttatt acgcaattct
     6541 tttttgatgt agagagttat ttacgttttc gtgatcgctg tgtttctatt gggattgatt
     6601 gtgaaattgt gccgggtatt ctgccggtga caaatttcaa gcagttgcaa aaaatggcag
     6661 cagtgactaa tgttaagatc ccgacttgga tgggtaaaat gtacgaaggg ttagatgatg
     6721 atcaaaccac cagaaactta gttgcagcaa gtattgcaat ggatatggtg aaagtgcttt
     6781 ctcgtgaagg ggtgaatgat ttccacttct ataccttaaa tcgtagtgaa ctcacttatg
     6841 caatgtgcca tactttaggc gtaagacctg atttaacaac tgcataatcg aacattaagg
     6901 ctgtatagct aaatgagcta tacctcctta tagttttact tctcttcagc cacccaaatc
     6961 tcgccatttt ttaataataa ataggctttg ttggaatcgg ctttggtttg tttaattcca
     7021 gccggcaggg cggttggcaa ttcagtgatc ttgcctgttt tatccaacac aacaacttgt
     7081 tgatagccca tttggggtat cagtgcgaaa gtctctgttg tcgccactaa attggtttcg
     7141 tgatcaccat agcggtgatt gcttaagccg gaggtaataa aacgttcagc taattggttt
     7201 cctttcaggc tgtattcaac caaattgcct tttagatgtg gcatttggat tgcatagagc
     7261 ttgcggttaa agataaacgg cgattgccaa cggttagtcg acagcggctc actttcggct
     7321 tctattgata attttccgtc tttaacgcca atcacaacag tgctggcccc attgccatcg
     7381 ccagagataa cagaaacgat cttggttttt ccattttctg tgaaagtcgc ccagcggttg
     7441 gcttcaaata ctcgcccttg ttgggtgagt ttttctgcta aggggtttag ggtatggcgt
     7501 tctagaaatt gaatttcgcc tgcctctaca tcatcgccta atacaccgtg ttgatatgta
     7561 aaatcatcag gctttgcaag tacagcaata tgcccttggt taggtgtttc ggcatcaaaa
     7621 tcaatttgca gagggtgagc atcaggcaac acctcggttt ctgcggtggc agtaatagtg
     7681 gcttggtttc cttgcgtttc aattctggct aacttaaatt gtccgttttg tttggtcacg
     7741 gcaatggtgg caaaattaag ggcggtcatt gtagctttcg gggcaagggg aatgttggag
     7801 gtatagattt tatcagccgt ccacagcata aaatgcccgt tttgatccgc taacgcaatt
     7861 cgttggtatt gagctttagg ttcaatctcg gtggaaactc catctgccag tttttgagct
     7921 tgttgtgtga ctctccataa ttgtttttgt tggttaatta ccactaattc gccttgttct
     7981 gaaaggtcaa attggcttac tttgccaatt tggggaagtg ggagtggaga aagctgaaca
     8041 tcagcaaaca aagaagcgga gaacaagctt aagattaaca gaatagattt tttcattagc
     8101 gttacctaaa aagaagattt aaatgataaa gggagatgtt atctcccttt acgctttagc
     8161 ctttataacc attcgggtta tttttttgcc aattccaagt gtctttcatc atttgttcta
     8221 gcccacgctc ggtttcccaa cccagttgct ctaacgcttt ttgtggagcg gagtagcaaa
     8281 cggcaatatc gcccgggcgg cgatctacca ctttgtatgg aatagtaatg ccatttgcgg
     8341 cttcaaaggc ttttaccata tctagcaccg aataacctgt tcctgtgcct aaattgtaaa
     8401 cgtggaaacc ggcatcattt tggtgcttgt ctaaggcttt taagtgacca agtgctaaat
     8461 ctaccacgtg gatataatca cgcacacctg tgccatcgtg agtattataa tcgccaccaa
     8521 acaccgataa ttgaggtaat ttgcccacag ccacttggct gataaacggc attaagttat
     8581 ttgggatacc gtttggatct tcaccgatta aaccgctttc gtgagcccct accgggttga
     8641 agtaacgcaa tactaccgca cttaattgtg ggaaggcttt aacggtatct tcaagaatgc
     8701 gttcaaccat atatttggac gtaccgtaag ggttggttgt gccaccgaca gggcaagatt
     8761 ccacaatcgg gataatctgc ggatcaccat aaacggtcgc cgatgaacta aacacaatgg
     8821 tattcacatt ggcttttagc atctcttcta ccaatacaat cgagccggtt acattgtttt
     8881 gatagtaacg tagcggttct ctgacgcttt cgcctacagc ttttaaaccg gcaaagtgaa
     8941 ttaccgattc aatctgattt tccgcaaaaa ttttgcgtaa aatatcacga tctaaaatat
     9001 cgccttgata gaattttacg ctcttgccgg tgatctgctt cacacgctca agtgatactt
     9061 cagaggaatt agaaagatta tctaacacca cgatttcacg attttcgttt aataattcaa
     9121 ctaatgtatg tgaaccgatg tagcctgcac cacctgtaac taaaattgcc attttatgtt
     9181 cctctataaa attaatacga accaaggtta ttgttgctat tttacccgat ttaatcaggc
     9241 ttgctaatat ttattcagtt tataatgcca ctccggcttc cggcagtaat tccggaatgc
     9301 cgtttttaat cgggtaagca atattgagct tagggttaat taatcggttg ttctctttgt
     9361 cccattctaa tttttcatta gagacagggc agacgaggtt attgagtagt gtttcgttca
     9421 tcttcttttc ctagtttttg tagaatcggg gctaacaaac aagcggtcga tttttctgaa
     9481 aatgttgcag aaaccggtac ataccaccaa tttggctgtg caaaatcgtg gcattttacc
     9541 gcatctttct cggtcattaa taacggcgtt tggctatcag caaattgagc cagctgagcc
     9601 ttatcaaagg cttgatgatc ggcaaagcct tgagttttgg caagcggaat gcctaatttt
     9661 tctaacatcg taaaaaagcg tggtgggtga ccgataccgg caatcgccac acagggttgg
     9721 gtaaattcgc ttaatggctt gcgttgattg gtgactaaat taattgcatc agacggttca
     9781 agcgtcatta aatgctcgtt agcttgcggt gtgccgccgt tgcaaataac cgcttgcacg
     9841 gagtttaaac ggctcggtaa ttcacgcaat ccgcccgccg gcagcacaaa accgttaccg
     9901 aaacgcctta cgccatcaac caccacccat tccatatctc gctgtagggc atagtgttgc
     9961 aagccatcat cggtgatgat gatgtccggc tcaaactgat ttaatagtaa ttcaatgctt
    10021 tgttggcggt ttggagaaat agccaaaggc acattggttc gctgaacaat cagaaccggt
    10081 tcatcaccca ccatttcagc agaactttct cgggtaacta attgcggaaa cgttttcgct
    10141 ttaccgccat agcctcgaga aatcacgcct acttttacgc cacgctgttg cagttgttcc
    10201 accagccaaa cgaccactgg cgttttgccg ttgcctccaa ccgagatatt accgactacc
    10261 agcataggca aaggcgaacg gtacgattgc aaaatattca gagaatataa ccgcttgcga
    10321 gcttgactaa tcagccaaaa cagcaacgaa agcggcgaaa gcagccaagt gataagggat
    10381 tttgtttgcc agattttcat attatttatt tttaagtgtt tcaactaaat ctcgtaataa
    10441 ggctaattca cgtttttggt tttcgataat ttcttcttta tgggctaata attcatcttt
    10501 gtgggagata ataagttgta aacgttgaat ttcttctact ccatttccta atgagataaa
    10561 tgaattagtg agatt
//
LOCUS       DANLYI010000073        10474 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.073, whole genome shotgun sequence.
ACCESSION   DANLYI010000073 DANLYI010000000
VERSION     DANLYI010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 10474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10474
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.073"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            complement(<1..969)
                     /locus_tag="PXD81_001329"
     CDS             complement(<1..969)
                     /locus_tag="PXD81_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt transporter"
                     /protein_id="HDL5537351.1"
                     /translation="MRLKATYWGMIVIGFLSLFALYQLYPMILFKVMEWQKNFNLQLS
                     ASLNELAENQSKAGITLVFISFLYGVFHAVGPGHGKFILSSYLSFEKTKLNQAMKITF
                     ASALVQGLVAISLVTVIVVIFTLSRQYFNLTLKWVERGSFGLMILFGIYWCYQVYRTV
                     NRARKPIIKSIRISQKKAGKLPLVTQKGHIHTENCSCRHKHLPTSNEMQQVRDWKAQL
                     MLVLSIGSRPCSGAILVLFLSYTLNLYAWGVISALAMAVGTGLTLSLFAYLVIIARNK
                     AVKVSQWYFSAQINRNIVWILKFTLGLALILFGMVLFHSSFIDAGTG"
     gene            complement(960..1598)
                     /locus_tag="PXD81_001330"
     CDS             complement(960..1598)
                     /locus_tag="PXD81_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1007 family protein"
                     /protein_id="HDL5537352.1"
                     /translation="MKFIKTIITLIALYSMPVFSHPHSFVDLKTNVIVEGSMLKSFQM
                     EWMLDEIASSELIYEVKNSQDKEKTQQNITAEMVQTALQNHYFSTLYDSQNQPIKFTT
                     KPTATRFEIKNNRVIFYITFHLAKPHELKQSKVRFYTYEPSYYIAMEYNRPADVNTSN
                     ASCKATLVQPQVDSKLRLYASGLDKNQSLDMPENGDYSLGAQFAQKVEIICD"
     gene            complement(1659..2037)
                     /gene="rnpB"
                     /locus_tag="PXD81_001331"
     ncRNA           complement(1659..2037)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PXD81_001331"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(2166..2453)
                     /gene="rplY"
                     /locus_tag="PXD81_001332"
     CDS             complement(2166..2453)
                     /gene="rplY"
                     /locus_tag="PXD81_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005625668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HDL5537353.1"
                     /translation="MSFKFEAEVRSAQGKGASRRLRHNGQVPAIIYGGNAEPVSIILD
                     HDKVNNAQVHDSFYNEVLTIVVAGKEEQVKVQAIQRHPTKPKLVHLDFKRA"
     gene            complement(2605..3873)
                     /locus_tag="PXD81_001333"
     CDS             complement(2605..3873)
                     /locus_tag="PXD81_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005600593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malic enzyme"
                     /protein_id="HDL5537354.1"
                     /translation="MSDLKQQALDFHEFPIPGKISVTPTKALDTQHDLALAYSPGVAE
                     PCLAIEKDPSASYRYTARGNLVAVISNGTAVLGLGNIGALASKPVMEGKGVLFKKFAG
                     IDVFDIEINETDPEKLVEIIASLEPTFGGINLEDIKAPECFYVEQKLRERMGIPVFHD
                     DQHGTAIITGAAVINGLQVVGKAISEVRLVVNGAGASAIACTNLLRSLGVKKENITMC
                     DSKGVIFQGRGDKMDETKQFYAIEDNGWRTLADAVANADVFLGCSKGGALTQEMVKTM
                     AKDLLILALANPVPEITPAEAKAVRPDAIVCTGRSDFKNQVNNVLCFPFLFRGALDVG
                     AKTINEEMKMAAAHAIAELAKEPVPFEVISTYGELSFGPEYIIPTPFDPRLIVNVSIG
                     VAKAAIQSGVATRPITDWEAYAEQLKKLVA"
     gene            complement(4030..4941)
                     /locus_tag="PXD81_001334"
     CDS             complement(4030..4941)
                     /locus_tag="PXD81_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RimK family alpha-L-glutamate ligase"
                     /protein_id="HDL5537355.1"
                     /translation="MKWLMLCREPRLYSCCRIKAACEAKGIELDILDQNRMLLGLENG
                     EFKIYYQAGESYDNNRPEPVLLPNYDGILPRFGTGSTEMGCSVLRHFEAKGIPVLNHS
                     AAFALARDKWRTLQKLAKHNLPVPNTNFAGHLVSVKSQLNQFAFPLISKVLNGSQGNG
                     VMLFEGKNNAEAVLATFRQVNEPYLCQQFVGDAKGQDIRAFVIGNEVVAAMSRTSVTG
                     DFRANIHQGGTAQPIELTQMERALAIKATKTIGLDIAGVDFLRTEKGVVILEVNASPG
                     FEGIERVNDVDIASEMVSYFINKMKNA"
     gene            complement(4938..5657)
                     /gene="nfsA"
                     /locus_tag="PXD81_001335"
     CDS             complement(4938..5657)
                     /gene="nfsA"
                     /locus_tag="PXD81_001335"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NADPH nitroreductase"
                     /protein_id="HDL5537356.1"
                     /translation="MQSKPTLETILSHRSIRKFTEQPISEEIFQILIEAGKAASSSNH
                     LQCVTVIRVTDRQIRETLKAVSGMQYVVDCAEFLVFCIDFNKHKQLVPESQLEWAEVS
                     LIGAVDTGILAQNVFLAAESLGLGAVYIGALRNDIERVSKALNLPEYCVPLVGMCLGY
                     PAQEPAIKPRLPNSLVCYNNQYQDFDWTEFAKYNENLTAYYQARIGEPQQWQAAIHKT
                     LDKPVRPHILPFFNQKGLLKK"
     gene            complement(5669..6454)
                     /gene="mtgA"
                     /locus_tag="PXD81_001336"
     CDS             complement(5669..6454)
                     /gene="mtgA"
                     /locus_tag="PXD81_001336"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HDL5537357.1"
                     /translation="MEQSMAKRNKRFLSSLLKKIGLFFIPRKIKSKLGWLWFGSSRLL
                     TFGGTFFLIPILIFSVLPVPYSAYMVQKKVENLVQGKSYPIKKEWVSLNEISWQMQMA
                     VIASEDQKFESHFGIDLQAIETALKLNAKSKKVRGGSTISQQTVKNMFLWHGQSWLRK
                     GIEVPLTLLVENVWSKDRILEVYLNIAEFGNGIFGVEAAAKHFFKKSAKQLTLQESAL
                     LAASLPNPLVFRVDKPGPSMRKRQAWIMRQVSLLDGKNYLDKL"
     gene            complement(6429..6749)
                     /gene="trpR"
                     /locus_tag="PXD81_001337"
     CDS             complement(6429..6749)
                     /gene="trpR"
                     /locus_tag="PXD81_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HDL5537358.1"
                     /translation="MKTLYNQRDPNEWTQFLALLEQAVAEDKLEPFFSMFLTADERGS
                     LGLRLQIVQALLKGEDSQREIQQNLNTSAATITRGSNMLKTLDQDFLQWLNGKVNGTV
                     NGKA"
     gene            complement(6829..7470)
                     /gene="lolA"
                     /locus_tag="PXD81_001338"
     CDS             complement(6829..7470)
                     /gene="lolA"
                     /locus_tag="PXD81_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005600572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HDL5537359.1"
                     /translation="MKTLFKKSVFGLALFGLANVALADAQSVAELQRRLEQVSQYSAD
                     FDQTVRSSKGKQIQQGKGKFQVKRPNLFRMDTKSPQENLIVSDGKDLWFYDPFVSQVT
                     VNTVQDAVNNTPFVLLTSGDKSHWDQYDVSQSADTFVLKPKSKKSNLKQFDVRIDQSG
                     LLKGFSTIERDGQSNLYVLRNITTGGVSSDLFKFSVPKGAELDDQRGGKKSKK"
     gene            complement(7581..10439)
                     /gene="polA"
                     /locus_tag="PXD81_001339"
     CDS             complement(7581..10439)
                     /gene="polA"
                     /locus_tag="PXD81_001339"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005611716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HDL5537360.1"
                     /translation="MATIAKNPLVLVDGSSYLYRAFHAFPPLTNRLGEPTGAMYGVLN
                     MLKSLISQVNPSHIAVVFDAKGKTFRDELFKQYKSHRPPMPDDLRPQIEPLHRIIKAL
                     GIPLISIEGVEADDVIGTLAVQAAKDGKDVLISTGDKDMAQLVNDHIMLINTMNNTLL
                     DREGVIEKYGIPPELIIDFLALMGDSADNIPGVKGVGEKTALALLQGIGSLEQIYANL
                     DQIATLSFRGAKNFAPKLEAEKANADLSYLLATIKTDVELDVTHDQLLTQPQDRAELA
                     QLFEHYEFKRWLNEVNQNANPVTQTSAEKVPNNYQVTQAVRNEENVANVVEIDRSKYE
                     TVDTEQKLSAWIEKLTAARLIAVDTETDSLDAMSANLVGISFALENGEACYIPLGHKQ
                     KAQPQQADMFGDEERSESPSSDELVKNQLNLTACLSKLKPILLNPEIKKIGQNIKYDL
                     TIFANYGIELQGVAFDTMIESYTQNSTGRHNMDDLAARYLGHKTIPFEELAGKGKNQL
                     TFDKIEIAKAAEYAAEDADVTMKLHQVLWEKLQQEPELVKLFDEIEMPLVGVLSRIER
                     NGVLIDPKKLLTQSAEIEKRLEEVETLVHAEAGQTFNLASTKQLQEILFEKLGLPVLK
                     KTPKGAPSTNEEVLDELAQQGHLVPKLLMEHRGLSKLKSTYTDKLPQLINKKTGRVHT
                     SYNQAVTATGRLSSSDPNLQNIPIRNEEGRRIRQAFVAREGYKIVAADYSQIELRIMA
                     HLSNDEGMINAFAEGKDIHRATAAEIFGVELGEVTSEQRRSAKAINFGLIYGMSSFGL
                     SNQLGIGRAEAQKYMDLYFQRYPAVQQFMTDIREVAVEKGYVETLFGRRLYLPDIKSG
                     NAILRKAAERVAINAPMQGTAADIIKVAMIGIDNAIRDNDEIKMIMQVHDELVFEVKA
                     DKIDHYSQLIKTEMEKAISLKVPLIAEVGVGENWDEAH"
BASE COUNT         2984 a         2384 c         1903 g         3203 t
ORIGIN      
        1 gcctgtacca gcatcaatga aactactgtg aaataatacc attccaaata gaattaatgc
       61 gagtccaagt gtaaatttta atatccaaac gatatttcga ttaatttgag ctgaaaaata
      121 ccattggcta accttaactg ccttatttct cgcaatgata acaagataag caaataagct
      181 taaagtcagc cctgttccta ctgccatagc taatgctgaa ataacccccc aagcatagag
      241 attgagcgta taagaaagaa ataaaacaag aatagcgcca gagcaaggtc ttgagccaat
      301 acttaatacc aacatcagct gtgctttcca atctctcact tgttgcattt catttgaagt
      361 cggtaaatgt ttatgcctac agctacaatt ttcggtatga atatgccctt tctgcgtgac
      421 aagcggtaat tttccagctt ttttttgcga aatacgaatg gatttaatga taggctttcg
      481 cgctctgttc accgttcgat acacctggta acaccagtaa atgccgaaca agatcatcaa
      541 accaaagctc ccccgctcaa cccattttag ggttaaatta aaatattggc gagagagagt
      601 aaaaatcacc acaatcaccg tcaccaatga aattgccact aagccctgca ccaatgctga
      661 ggcaaaagtg attttcatcg cctgatttaa ttttgttttt tcaaaagaaa gataactgct
      721 cagaataaat tttccgtgac caggcccaac tgcgtgaaat actccatata aaaaactaat
      781 aaatactaag gtaataccgg ctttactttg attttcagct aattcattga gcgaagcgga
      841 aagctgtaaa ttaaagtttt tttgccattc catcacttta aacaatatca ttggataaag
      901 ctgataaaga gcaaataggc tcaaaaatcc tatcactatc attccccaat atgttgcttt
      961 taatcgcata taatctccac tttttgagca aattgggcac caagagagta atctccattt
     1021 tcaggcatat ccagtgattg gtttttatcc aaaccggaag catataagcg taacttacta
     1081 tctacttgag gttgcaccaa tgttgcttta caacttgcat tactggtatt gacatcagca
     1141 gggcgattat attccatcgc aatataataa ctcggttcat aagtatagaa tctcacttta
     1201 ctctgcttta attcgtgagg tttagctaaa tgaaaagtaa tataaaaaat cactctgtta
     1261 ttcttgattt caaagcgagt cgctgtgggc tttgtcgtaa attttatcgg ctgattttga
     1321 ctatcatata aggtgctaaa ataatgattt tgcaatgcgg tttgcaccat ttctgcagtg
     1381 atattctgct gagttttttc tttatcttgg ctatttttca cttcataaat taactcagaa
     1441 gaagcaattt catctagcat ccattccatt tggaaagatt tcaacattga gccttccaca
     1501 ataacattcg ttttcaaatc aacaaatgaa tgtgggtgag aaaaaactgg cattgaatat
     1561 aatgcaatta acgtgataat cgtcttaata aatttcatat ttactttcac tataaaataa
     1621 aaaagcgaac aagttccccc tgttcgcatt tcaagaattt agtgagttgg tcgataagcc
     1681 gagttctgtc gtggacaatc attcctctag acgtacattt acacatacgc tcaagcaacc
     1741 tacccgaact ctcagcgggc caccgattga gttcctattt ggtcttgcta cgagtggagt
     1801 ttaccctgct gtggaatgtt accacccaca cggtgcgctc ttaccgcacc ctttcaccct
     1861 taccgacaaa gccggcggtc tgctctctgt tgcactggtc gtaggctcac gccccccgga
     1921 cgttatccgg cactctgccc tgtgtagctc ggactttcct ctcgtttact tgtcccccag
     1981 tttacgtttt cacgaattta gaggcttcaa taaaccagcg attgcctaac caactcagca
     2041 ctagagtata atgctttcgc aaaaaattgc aaggaaataa ccgcttgtag ttattatggc
     2101 aaaataaaaa aggattaagt tcatttaaaa acttaatcct cttcgcttta attcactcgt
     2161 aaaaattacg cacgtttgaa gtctaagtga actaattttg gtttagttgg gtggcgttga
     2221 attgcttgta cttttacttg ctcttcttta cctgcaacaa cgatagttaa tacttcgtta
     2281 tagaatgaat cgtgaacttg tgcgttgttc actttatcgt gatctaaaat gattgataca
     2341 ggctctgcat taccaccgta gatgattgca ggaacttgac cattgtgacg caggcggcgg
     2401 ctcgcaccct taccttgcgc agaacgaact tcagcttcaa atttgaatga catagtttta
     2461 ttctcttaaa taaaatgggg aaatccccgt gaatagaaaa aacagcaggc gacccagctg
     2521 tttccgaaaa aatatcggca tatccgtctc gatatgccaa ctacgtattt tactgaagtt
     2581 gcagaaaaat gcaatgtaaa aatattaagc aaccagtttt ttcaactgct ctgcataagc
     2641 ttcccaatct gtaattggtc gagttgctac acctgattga attgctgctt ttgctacacc
     2701 aatcgagaca ttcacgatca aacgtggatc aaatggcgtt ggaataatat attccgggcc
     2761 aaacgataat tcgccataag ttgaaataac ttcaaacggc acaggttctt tagccaactc
     2821 tgcaatcgca tgggctgctg ccattttcat ttcttcatta atggtttttg ccccaacatc
     2881 taatgctcca cggaataaga acgggaaaca aagcacatta ttcacttggt ttttaaagtc
     2941 tgaacgaccg gtacatacaa tcgcatctgg acggacagcc ttcgcttcag caggtgtaat
     3001 ttccggcaca gggttcgcta aagcaagaat taatagatct tttgccattg ttttaaccat
     3061 ttcttgcgtt aaagctccgc cttttgaaca acctaagaaa acatctgcat tcgcaacggc
     3121 atctgccaat gttctccaac cattatcttc tattgcatag aattgtttgg tttcatccat
     3181 cttatcacca cgaccttgga aaataacgcc cttagaatcg cacattgtga tattttcttt
     3241 tttcacacct aatgaacgga gtaagttagt acacgcaatc gctgatgctc cagcaccgtt
     3301 taccactaaa cgtacttctg aaattgcttt acctaccact tgtaatccgt taatcaccgc
     3361 cgcaccggta ataattgccg taccgtgttg atcatcgtgg aacacaggga ttcccatacg
     3421 ttcacgtaat ttttgctcta cataaaaaca ttcaggtgct ttaatatctt ccagattaat
     3481 accaccaaaa gtcggttcta acgatgcaat aatctccact aatttctccg gatcagtttc
     3541 attaatttca atatcaaaca catcaattcc ggcaaatttc ttaaatagaa cacctttgcc
     3601 ttccatcaca ggcttacttg ctaaagcacc aatattgcct aagcctaaca ctgctgtacc
     3661 attagaaatt actgcaacta agttacctcg tgccgtataa cgataagaag ctgaaggatc
     3721 tttttcaatc gctaagcaag gctcagcaac cccaggagag tacgctaaag ctaaatcgtg
     3781 ctgagtatct aatgctttag taggagtaac ggaaattttt ccggggattg ggaactcgtg
     3841 aaaatcaagc gcctgttgtt ttaaatcgct cattttagaa accttataat ctataaaaat
     3901 aaaagtaatc aataatagcc aaagagtagc cattttaccc taaatttttt cgcattttta
     3961 tggctttgct ctcatttttt atgatattta caaaaaataa tacaaaaacg tccgcttgtt
     4021 ttactcaaat caagcattct tcatcttatt gataaaataa gaaaccattt cagaagctat
     4081 atcaacatca tttactctct ctatgccttc aaatcctggg ctggcattga cttctaaaat
     4141 aactacgcct ttctctgtcc gcagaaaatc aacgcctgca atatcaagcc ctatcgtttt
     4201 agttgcttta attgctaacg cacgttccat ctgagttaat tcaatcggtt gagccgttcc
     4261 accttgatga atattggctc taaaatcgcc tgttacgctg gttcggctca tggcagcaac
     4321 cacttcattg ccaatcacaa acgcccgaat atcttgccct ttagcgtcgc ccacaaattg
     4381 ttggcataga taaggctcat ttacttgcct aaaggtcgct aacacggcct cggcattatt
     4441 tttgccttca aatagcatta cgccattccc ctgtgaacca tttagcactt ttgaaataag
     4501 tggaaaagca aattgattga gttgtgattt cacagaaacc aaatgccctg caaaattcgt
     4561 attaggaacc ggcaaattgt gtttggctaa tttttgtaat gttcgccatt tgtctcgggc
     4621 taacgcaaat gccgctgaat gatttagaac aggaatacct ttcgcctcaa aatggcgtaa
     4681 caccgaacag cccatttcgg tactgcctgt gccaaaacgg ggtaaaatgc cgtcataatt
     4741 cggcaacaat accggctccg gacggttgtt atcatagctc tcgccagctt gatagtagat
     4801 cttaaattcg ccattttcta acccgagtag cattctattc tggtctaaaa tatccagctc
     4861 aatccccttt gcctcacaag cggcttttat tcgacagcag ctatataaac gaggctctcg
     4921 acatagcatt agccacttca ttttttcagc aatcctttct gattgaaaaa cggcaaaata
     4981 tgcggacgaa caggcttatc caatgtttta tgaatagctg cttgccattg ttgtggctcg
     5041 ccgattctgg cttggtagta agcggtcaaa ttttcattat attttgcaaa ttcagtccaa
     5101 tcaaaatcct gatattgatt gttataacaa accaaactat tcggcaaacg aggttttatt
     5161 gccggctctt gtgccggata ccccaaacac atacccacca gtggaacaca atattccggc
     5221 aagttaaggg ctttagaaac ccgctcaata tcattacgca atgctccaat atacaccgct
     5281 cccagcccta gtgattcggc ggctaaaaat acattctggg ccaaaatacc ggtatctacc
     5341 gccccaatca acgacacctc cgcccattcc aactgcgatt cagggacaag ttgtttgtgt
     5401 ttattaaaat caatgcagaa caccaaaaat tcagcacaat ctactacata ttgcatacct
     5461 gaaacggctt ttaatgtttc tcgaatctgc cgatcggtta cacgaatcac cgtcacacat
     5521 tgcagatgat ttgaacttga agccgcttta cctgcctcaa ttaagatttg gaaaatctct
     5581 tcagaaatag gctgttcggt aaatttacga atagaacggt gagataaaat cgtctcaaga
     5641 gtcggtttac tttgcatatt tttctcgtct aaagtttatc taaataattt ttgccatcaa
     5701 gcaaacttac ttgtcgcata atccacgctt ggcgttttcg catactcggg ccgggtttat
     5761 ctaccctaaa caccaacggg ttaggcaaag aggctgccaa tagtgcggat tcttgcaagg
     5821 ttagctgttt ggctgatttt ttgaaaaaat gctttgcggc tgcttccacc ccgaaaatgc
     5881 cgttaccaaa ttccgcaata ttgaggtaca cctctaaaat ccgatcctta ctccacacat
     5941 tttcgactaa cagggttaaa ggcacttcaa tccctttgcg taaccagctt tgcccgtgcc
     6001 acaaaaacat attttttacc gtttgttgcg aaatggttga acctccacgc acttttttgg
     6061 atttagcatt cagtttcaag gcagtttcta tcgcttgtaa gtcaatgcca aaatggcttt
     6121 caaacttttg atcttctgag gcaatcacag ccatttgcat ttgccacgaa atctcattca
     6181 ggctcaccca ttcttttttg atcgggtaag atttcccctg aaccagattc tccacctttt
     6241 tttgcaccat atacgcagaa taaggcaccg gtaacaccga aaaaatcagt atcggaatca
     6301 ggaaaaatgt gccaccaaag gttaataagc gactagaacc aaaccaaagc cagcccagtt
     6361 tactttttat tttgcgagga ataaaaaaaa gccctatttt cttcaaaaga gagcttaaaa
     6421 atcgcttgtt acgctttgcc attgactgtt ccattaacct tgccgttcag ccattgcaga
     6481 aaatcttggt cgagtgtttt aagcatattt gaaccacgag tgatggttgc cgcactggta
     6541 tttaaatttt gttggatttc ccgttgcgaa tcttcacctt ttagcaaagc ttgcacaatt
     6601 tgtaaacgca agcctaatga acctcgctca tcggcggtga gaaacattga gaaaaacggc
     6661 tcaagtttat cttccgcaac cgcttgttca agcagagcca aaaactgcgt ccactcgttt
     6721 ggatctcgct ggttatagag tgttttcata ctgtcccttg aagtaaaaac ggtgacaagc
     6781 ggtcagattt ttacaaaatt ttgcagaaaa acgaccgctt gctctccatt attttttcga
     6841 ttttttaccg ccacgttggt cgtctaactc tgcccctttc ggtacgctga atttaaacaa
     6901 atcgcttgag acaccgccgg tagtaatatt gcgtaacaca tataagttac tttgcccgtc
     6961 acgctcaatg gtactgaaac cttttaataa accgctttgg tcaatccgca catcaaactg
     7021 ttttaaattg ctttttttcg attttggctt aagcacgaaa gtatcagcac tctggctgac
     7081 atcatattga tcccagtggc ttttatcacc gctggttaat aagacgaatg gcgtattatt
     7141 aactgcatct tgcacggtat ttaccgtaac ttgtgaaacg aaaggatcgt aaaaccataa
     7201 atctttgccg tcagatacaa tcaaattttc ttgtggtgat ttggtatcca ttctaaataa
     7261 gttcgggcgt tttacttgga atttgccttt gccttgctga atttgtttac ctttgctaga
     7321 acgcacggtt tggtcgaaat ccgcactata ttggcttact tgttccaaac ggcgttgtaa
     7381 ttctgcaaca gattgagcat cagccaatgc cacatttgcc aagccaaata acgctaaacc
     7441 gaataccgat tttttaaata atgttttcat ttgttttcct ttttaccaac aagaattttc
     7501 ttgtttgaca ataaaatcat caaaagtttc atattaatga actgttacaa caatacaata
     7561 aaagtcgtga attgtatagt ttaatgagct tcgtcccagt tttcgcctac gccaacttcg
     7621 gcaatcagtg gcactttgag gctaatcgct ttttccattt cggttttgat taattggctg
     7681 taatggtcga ttttatcggc tttgacttca aacaccaatt catcgtgaac ctgcataatc
     7741 attttgattt catcattatc acgaatcgca ttatcaatac caatcatcgc caccttaata
     7801 atatcggcag ccgtgccttg cattggggcg ttgattgcca cccgttctgc tgctttacgc
     7861 aaaatagcat tgccggattt aatatcaggc aagtacaaac ggcgaccaaa tagggtttca
     7921 acatagcctt tttcaactgc aacttcacga atatcggtca taaattgttg aaccgccggg
     7981 taacgttgga agtagagatc catatatttt tgagcctctg cacgcccaat ccctaattgg
     8041 tttgaaagcc caaatgacga cattccgtaa atcagcccga agttaattgc tttcgcactg
     8101 cggcgttgtt cggaggtgac ttcacctaac tccaccccga aaatttcggc agcggtagca
     8161 cggtgaatat ctttgccctc cgcaaaggcg ttaatcattc cctcatcatt cgataaatga
     8221 gccataatcc gtagctcaat ttgtgaatag tcggcagcga caattttata accttctctc
     8281 gcgacaaaag cttggcgaat ccgtctgccc tcttcattgc ggatcggaat gttctgcaaa
     8341 ttcggatcgc tggaagaaag gcgtccggtt gcggtgactg cttgattgta agaggtatgc
     8401 acccgccctg tttttttgtt aatcagttgt ggtaatttat cggtgtaggt agatttcaac
     8461 ttacttaacc cacgatgctc cattaataac ttcggcacta aatgcccttg ttgagccaac
     8521 tcgtctaaca cttcttcgtt ggttgagggt gcaccttttg gagttttctt caacaccggc
     8581 aagcctaatt tttcaaacaa aatctcttgt aattgtttag ttgaggcaag attgaaggtt
     8641 tgccctgctt ctgcgtgtac taatgtttca acttcctcca aacgtttttc aatttcagcc
     8701 gattgggtca ataatttttt cgggtcaatc aacacgccat tgcgttcaat tcgagaaagt
     8761 acgcccacaa gtggcatttc aatttcatcg aataacttca ccaattcagg ctcttgctgt
     8821 agtttttccc acaatacttg gtggagtttc atcgtcacat cggcatcttc tgccgcatat
     8881 tcagccgctt ttgctatctc gattttatcg aaggtcagtt gatttttccc cttaccagca
     8941 agctcttcaa acggaatcgt tttatgcccc aaataacggg cggctaaatc gtccatatta
     9001 tgacgaccgg tgctgttttg ggtgtaagat tcaatcatcg tatcaaaagc aacaccttgt
     9061 agctcaatgc cgtagttcgc aaaaatggtt aaatcatatt taatattctg cccgatcttt
     9121 ttaatctccg ggttaagcaa aatcggcttg agtttactta aacaagcggt caaatttagc
     9181 tgatttttta ccaattcatc ggaagacggg ctttctgaac gctcctcatc accgaacata
     9241 tcggcttgtt gcggttgggc tttttgctta tgcccaagcg gaatatagca cgcctcgcca
     9301 ttttccagtg caaaggaaat accgaccaaa ttagccgaca ttgcgtcaag gctgtcggtt
     9361 tcggtatcca ccgcaatcaa acgagctgcg gtgagttttt cgatccaggc tgataatttc
     9421 tgttcagtat caacggtctc atatttgcta cgatcgatct cgaccacatt tgcaacattt
     9481 tcctcatttc tgaccgcttg tgttacttgg taattattcg gtactttttc agccgaagtt
     9541 tgagtgaccg gattggcatt ttgattcact tcgttcagcc agcgtttaaa ttcataatgc
     9601 tcaaataatt gagcaagctc agcacggtct tgcggctgtg ttaaaagttg atcgtgagta
     9661 acatcaagtt ccacatcggt tttgatggta gcaagcaaat aggataaatc ggcattcgct
     9721 ttttccgctt ccaattttgg ggcgaaattt tttgccccac ggaaagaaag ggtggcaatt
     9781 tgatccaaat tagcataaat ctgttcaagt gaaccaatgc cttgcaataa agctaatgcg
     9841 gtcttttccc ccacaccttt tacgcccgga atgttatcgg cggaatcgcc cattaatgcc
     9901 agaaagtcga taatcaactc aggcggaatg ccatattttt caatcacgcc ttcacgatct
     9961 aacaaagtat tgttcatcgt gttaatcaac ataatgtgat cgttcaccaa ttgtgccata
    10021 tctttatcgc cggtgctgat caacacatct ttgccgtctt tagcggcttg cacagcaagc
    10081 gtgccgatca catcatcagc ttccacacct tcaatcgaaa tcagtggaat gcctaaggct
    10141 ttgataatgc gatgcaaagg ctcaatttga gggcgtaaat catcgggcat tggtgggcgg
    10201 tgcgatttat attgcttaaa caactcatca cggaaggttt tccctttggc atcaaacacc
    10261 acagcaatat ggcttggatt cacttgtgaa atcaggcttt ttaacatatt cagcacgcca
    10321 tacattgccc ctgtcggttc gcctaagcga ttagtgagcg gagggaaagc gtgaaatgct
    10381 cgatataaat aggaagaacc atccaccaat accagtggat tttttgcgat agttgccata
    10441 aaaatctctt tataatcaaa taaatattgg cgtt
//
LOCUS       DANLYI010000074        10306 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.074, whole genome shotgun sequence.
ACCESSION   DANLYI010000074 DANLYI010000000
VERSION     DANLYI010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 10306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10306
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.074"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            complement(<1..704)
                     /locus_tag="PXD81_001340"
     CDS             complement(<1..704)
                     /locus_tag="PXD81_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HDL5537361.1"
                     /translation="MENFEKQFISLFQRIVPHYRRSEVFYDFITLSALDMYLVTYRDQ
                     AEPNLRERFAHAKARYSDSEFTQLAELLAVTVNALTQKRYDFLGSVFMNLNLGDGYRG
                     QYFTPSHIADLMAKVTLQDCDRIISQQGFVTLSEPTCGSGVMVIGCVNAMFDAKYNPQ
                     QQLWVHCQDVDFTAAMMCYIQLSLLHIPACIVVGDTLLNETKIQMYTLAHLMGNWSHK
                     LEQQIAPLVNSTTEKG"
     gene            complement(777..1523)
                     /locus_tag="PXD81_001341"
     CDS             complement(777..1523)
                     /locus_tag="PXD81_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012340806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5537362.1"
                     /translation="MPNWCENKLQIFCDCIQAKQIIPKLFRKTESDKWCLDFELLVPM
                     PESICIESSSLGDRAMEFLKISPNRRLTRPLLKKYIYGSEIDRIYAKAKHHRWDVGHF
                     INWLIKRPEQQSILWLNLALGQQYLSNLAQHGYKDWYDWSNEQWGCKWNVCSDSCQVS
                     FNDNGSINCLFDTPWGPPDGWFQALCTAFPDIEFILSFFEPGMWFAGKYIANLDGSYC
                     NIYIDDRGIRSFAEEVFNEEFEDEFEEKLE"
     gene            complement(1663..2637)
                     /locus_tag="PXD81_001342"
     CDS             complement(1663..2637)
                     /locus_tag="PXD81_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012340807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1738 domain-containing protein"
                     /protein_id="HDL5537363.1"
                     /translation="MNTQTKFDLYQHITDRIVAALEQGTAPWLKPWSHPECNMALPRN
                     AVSNRLYSGINTLLLWIATAEYGYKQSKWITAKKANELGGYIRKGEKATVVMNYRPDK
                     REKLDEEGNIVYDEEGNPEIEQHFFIKKHNLFNLDQCENLPEELYDPISEPKQPTSDP
                     NQYEIFADIRQIIKGMDLHVEMKPSNRAYYQPSADLVVMPEIKQFENAQAFHSVLLHE
                     MTHATGAAKRLDREGITSGKAKFGNKVYAFEELVAEMGGAFLCAYLGFDTVPQNAAYI
                     ESWIRVIKEDKRAIVRASGKAREACQYMLDALNISQLADKNEVFEEAA"
     gene            complement(2882..3289)
                     /locus_tag="PXD81_001343"
     CDS             complement(2882..3289)
                     /locus_tag="PXD81_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemophilus-specific protein"
                     /protein_id="HDL5537364.1"
                     /translation="MKHDLDLLKYIWDNRNLNNKAYQYLFHICIEPMRKGDWNRYTIW
                     LNNNGNLVNISAVVYEIVSSQQLKDDIKLWQDMQFHCRPVKVETTGFISFLLHQFFNG
                     ITHVLLEQGMITETEAAEKSYHFIAANSFQRLL"
     gene            complement(3405..4247)
                     /locus_tag="PXD81_001344"
     CDS             complement(3405..4247)
                     /locus_tag="PXD81_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012340809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5537365.1"
                     /translation="MSGSVLNTYFKHALVKAGFPNCLNIEYSLSHCQGDGVAFYGRLN
                     TETLINLFNKLNPQKRTQKMFSNLLNHIESWEPHYGVDFEIIRNSFGYRYSHCNTMEL
                     SVRTADDLAFFYDRDARKEWYFPTSKVEKYKALWDGFIQDLGQYIKDTSCALESNGYQ
                     IIEASPYQTETIFKFNTENYLIELKRKSSEFYSESVDWLCGDVCDFEAMIEEILQGKL
                     CYADFVATVTDKESGIQLGSDEITGVSYSPTDHSLSGYRQELISNAIASARSNAARFA
                     YLHA"
     gene            complement(4325..4720)
                     /locus_tag="PXD81_001345"
     CDS             complement(4325..4720)
                     /locus_tag="PXD81_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012340810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5537366.1"
                     /translation="MARKKIQYTETDLHLYSVLWNLRHAHDLFSVKLERETKFGKAFS
                     VFSRFDVCPSYGEVDITYLVVKAGIRKTLRGFAYVGGNGFSHSRHLMSTFMYLVREIL
                     VEHNWISETELYDQPISVYKFRAINFDKW"
     gene            complement(4819..5064)
                     /locus_tag="PXD81_001346"
     CDS             complement(4819..5064)
                     /locus_tag="PXD81_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012340811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemophilus-specific protein"
                     /protein_id="HDL5537367.1"
                     /translation="MLVHIRLSCIGDVLPTKLNEWTSEHQALQQEISHRALEAWQNRD
                     KDIKSVRFMQQMPSRQGLNFRFLSVSEQNGKLIVSRA"
     gene            complement(5136..5489)
                     /locus_tag="PXD81_001347"
     CDS             complement(5136..5489)
                     /locus_tag="PXD81_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5537368.1"
                     /translation="MSQQTAGWDVIPFESSKTVKPSLFWKLGNKLGNIFVSAKHKAKS
                     KYVEVTKGINQRIAEEDEMRALELQMCLDEQAAEYEQEISRIKRKMFFWTLLAAIIAF
                     VCGMAAVFGTQLSLI"
     gene            5863..6306
                     /locus_tag="PXD81_001348"
     CDS             5863..6306
                     /locus_tag="PXD81_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012340813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03757 family integrating conjugative element
                     protein"
                     /protein_id="HDL5537369.1"
                     /translation="MKVSRFLGYCSIVTGVFSPLAQAQPNIIVYTTKMYPIQNSQLAS
                     QIYYLDQVENFEESISRNLSKSPEIAEQEMRAFFNSADWKAQEQQLKDAYQGVISGWQ
                     NGIKKVPAVFFDNPQSSPSVIYGTTDVQKALDEWHKWVKQQGESK"
     gene            6303..7244
                     /locus_tag="PXD81_001349"
     CDS             6303..7244
                     /locus_tag="PXD81_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03756 family integrating conjugative element
                     protein"
                     /protein_id="HDL5537370.1"
                     /translation="MKKTFLTILISASLAVPTTLYPQPARASLNTASIAASSASTSCL
                     EYKVVGTCFWLFCTYFGCKVRTSTKIKHYLPEMVVSSYNHDGQTPWTELQALNPGVKG
                     GEYRSPQRQYSQLTFKNADAFGHPQGAIGQLLGNTGYYCKTQTTPFMPYFLSGLDFFA
                     WRENMPEMFYPEALTPGMREVGRVGDSWGNIYPRSGWVTQAHDYKASATIVQRVADIV
                     SRTAQPHIYFPAAKTGGAGEWPPGPVEEGQARTHKWQILYPQMSNTCSVFPNGGQTDT
                     YSSQISNQGDYAWALWRPYSCCKRRGQTFLYSIDWSH"
     gene            7262..9274
                     /locus_tag="PXD81_001350"
     CDS             7262..9274
                     /locus_tag="PXD81_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrating conjugative element protein"
                     /protein_id="HDL5537371.1"
                     /translation="MKSHFNFKKSHLRITTALLSSVSGLSYADSITDKLEFSKSDSII
                     SDRVFYQIGGGMGYMAPPSRGNTKVVDLGIGWKANLTCGNFDIKTTIKNDLNGLKDGF
                     KDLMGNVIDSAKGAVASMPAMVLQRANPQLYDLLTNGMYQGKLDFNRLKTSCEEMSKV
                     LADHTLGGKWGQAADLESYKQITSSQPDAKQAQKDLEDKKGKDGRVWIGGQKKGGENQ
                     EAINVVGDVVKAGFNLLNKRNALDTSTISGSSCDGNLCQTWDNPQTAADFLTKIIGEQ
                     SISTCKEDCGTKTSSKAGVGLSPEIEEEHVRTIVKLENVLNMDVPKAELLADLSSTTV
                     PVTRGLIESLRDDPDVEVLSQRLSAEIATSKIMEKMLLARRAVLAGMREPNVAQNADA
                     QVELERVLNIIDREISQVKLEMDMQKMITGNTASVILQSKSEREALAGAHSLSQDSLD
                     KRVQGLENGNVALTDLEAGSNINLSRRNIALSIPNIPNVAALIPNLPNRGGSGVGGNF
                     PSLAQISGSTMDQATGLLRNFEGFISKAQWDVNAHRVGYGSDTITRADGTIVKVTKDT
                     VITKEDAERDLARRTAIFANNVRKELGDSNWNALPPNAQAVLVSYAYNYGSLAKTKSV
                     LDAARRSAQSGDMNALATAIRNRQVDNNGINARRRNQEADYILGKN"
     gene            9295..9723
                     /locus_tag="PXD81_001351"
     CDS             9295..9723
                     /locus_tag="PXD81_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012340817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5537372.1"
                     /translation="MRKLLGITVLTLCTTSAFATDKNVVFSCTSTEGKPLTVKRVGND
                     YEYSYDKTIFKNPIKKAVTNDGSIIARGSGFTTYALELKNDGLKYVVGFVQPNGNSKE
                     FIEPGATISRDNDQPSIGSVDCDPYKKIYYKFDVHLMNTL"
     gene            9939..>10306
                     /locus_tag="PXD81_001352"
     CDS             9939..>10306
                     /locus_tag="PXD81_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012340818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5537373.1"
                     /translation="MDITTQNIELRYLSEQFYLDVAPHLKEILLKETRPYCVLLVRVK
                     NLDFALPLRSNLPEREGVGIKTIPNPQEYGKFKGIDFSKAILLTDKSYLKTDTVQLKN
                     KSELLNIQGNERRIVREFKK"
BASE COUNT         3264 a         2016 c         1986 g         3040 t
ORIGIN      
        1 cagcctttct cagtagtgct atttacaagt ggtgcaattt gttgctccag tttatgagac
       61 caatttccca tcaaatgagc cagcgtatac atttgaattt ttgtttcatt caatagcgta
      121 tcaccaacta caatacaagc aggaatatgt aataacgata gctggatata acacatcatg
      181 gcagctgtaa aatcaacatc ttgacaatga acccaaagtt gttgttgggg attgtatttc
      241 gcatcaaaca tcgcattaac acaaccaatc accattacac ccgaaccaca agttggttct
      301 gataaagtaa caaatccctg ttgtgagatg attctatcac agtcctgtag tgtaactttc
      361 gccattagat ctgcgatgtg agatggtgta aagtattgcc ctctataacc atcaccaaga
      421 ttgagattca taaatactga gcctaagaaa tcatagcgtt tttgtgttaa agcattcacc
      481 gtaacagcta acaattctgc taattgagta aactcagaat ctgaatatcg tgccttagca
      541 tgagcaaagc gttctcgcaa attaggttct gcttgatctc gatacgttac taggtacata
      601 tcaagtgcag agagtgtaat aaaatcataa aacacttctg aacgtcgata atgtggtacg
      661 atacgttgaa ataagctgat aaattgcttt tcaaagtttt ccattagact actccttaaa
      721 gtttacggag taatccccct agggaattac ccctagggtg gaaaaattaa ttaaggctat
      781 tcaagtttct cttcgaactc atcttcaaat tcttcattaa agacttcctc agcaaaactg
      841 cgaatacccc tatcatctat gtaaatattg cagtatgagc catcaagatt tgcgatatat
      901 ttgcccgcaa accacattcc cggctcaaaa aatgaaagta taaactcaat gtcaggaaag
      961 gcagtacaaa gtgcttggaa ccaaccatca ggtggtcccc atggcgtatc aaataggcaa
     1021 ttaatagatc cattatcatt aaagctaact tgacagctat ctgaacatac attccattta
     1081 cagccccact gttcatttga ccagtcatac caatctttgt aaccatgttg agctaggtta
     1141 gacagatatt gctggcctaa tgcgaggttt agccataata tactttgttg ttcaggacgt
     1201 tttatcaacc agttgataaa atgacctaca tcccagcgat gatgctttgc cttagcatat
     1261 atccgatcaa tttcggaccc atagatatac tttttgagta gagggcgtgt taaacgtcta
     1321 tttggtgaaa tttttagaaa ttccattgct ctatcaccaa gactacttga ttcaatgcag
     1381 attgattctg gcattggaac gagtaattca aagtctaaac accatttatc actttcagtt
     1441 tttctgaaaa gcttaggaat aatttgttta gcttggatac aatcgcaaaa aatttgtagt
     1501 ttattttcac accagtttgg cataatgtac tcctaaataa agtaggggta cacttgcctt
     1561 gaggcaatgt accccaaggg ttaaaagata atcgatacat aattcctaaa ggattatgta
     1621 ttactatgaa agattgtgtt gaaagtacct ttcagcaaag agttaagctg cttcttcaaa
     1681 gacctcgttt ttatcagcca gctgtgagat atttaatgca tctaacatat attgacaagc
     1741 ttcacgagct ttaccagaag cccggacaat agcccgttta tcttctttta taactcttat
     1801 ccaactttca atatatgccg cattttgagg taccgtatca aatccaaggt aggcacacaa
     1861 aaatgctccc cccatttcgg caaccaactc ctcaaaagca tagactttgt ttccaaattt
     1921 tgcttttccg gaggtaatac cctcacgatc gagcctttta gcagcaccag ttgcatgagt
     1981 catttcgtgc aataacacac tatgaaacgc ttgagcattt tcaaactgtt ttatttccgg
     2041 cattacaact aaatctgcac tgggttgata atatgcccga ttactcggtt tcatttccac
     2101 gtgtaaatcc attcctttga taatctgccg aatatcagca aaaatctcgt attggtttgg
     2161 atccgatgta ggctgtttag gttctgatat aggatcgtac agttcctcgg gtaaattttc
     2221 acactggtct agattgaaca gattgtgctt cttaatgaaa aagtgttgtt caatttctgg
     2281 attaccttcc tcgtcataca caatatttcc ctcctcatca agtttttctc tcttatcagg
     2341 acggtagttc attacaacag tagctttttc acctttacgg atatatccgc cgagctcgtt
     2401 agcttttttt gctgtaatcc acttactctg tttgtaaccg tattctgcgg tagcaatcca
     2461 gagtaatagt gtgttgatac cagaatacaa tcggttagag accgcattac gaggtaatgc
     2521 catattacat tctggatgac tccacggttt taaccatggt gcagtcccct gttccagtgc
     2581 tgcaacaatt cggtctgtga tatgttggta taaatcaaat ttagtttgag tgttcattag
     2641 tgtttctcca aagttcaggt agaaaatggg gaaacactat tacccttatc gggcactagt
     2701 ttccccgatg ggtggtttaa aatctcaaca gagatagttt ttctaggttc atcaaaacca
     2761 gtagaactgc ctataatgca gtaactacag ataatttgtt aatcagtatt gattcaagtt
     2821 aaacataatc ctcatatcga aaattacttt ggtaatacaa agttacatta tttcagatcc
     2881 attaaagtaa acgttgaaag ctattagcag caataaagtg ataacttttt tccgcagctt
     2941 ctgtttccgt aatcattccc tgctccagca aaacatgggt gattccattg aaaaactgat
     3001 gtaatagaaa tgaaataaaa cctgttgttt caacttttac tgggcgacaa tgaaactgca
     3061 tatcctgcca aagtttaata tcatctttta actgttgaga gctcacaatt tcatatacaa
     3121 cagcagaaat attaactaaa ttaccgttgt tattcagcca aattgtataa cgattccaat
     3181 ctccttttct cattggctct atacaaatgt gaaataggta ttggtaggct ttattattta
     3241 aattgcggtt atcccagatg tatttgagta aatctaaatc gtgtttcata atgtttctcc
     3301 aaaagaaaaa agtggagaaa catcttacct atagggaaaa tatttcccca cagggtaaaa
     3361 aacaatctcg ataagcggtc aaattgaaat aaaaaaccta caaatcaggc atgcaagtag
     3421 gcaaaacgtg cggcattact acgagcagaa gctatagcat tactaataag ctcttgtcga
     3481 taaccactca atgaatggtc tgtcggcgaa tagcttacgc ctgtaatttc atcgctacct
     3541 agttgaatgc cactttcctt atcagtaacg gttgctacaa aatcagcata gcaaagtttc
     3601 ccttgtagga tttcctctat cattgcctca aaatcgcaga catcaccaca tagccaatca
     3661 acagactctg aataaaattc agaggattta cgtttaagtt ctatcagata gttttccgtg
     3721 ttaaatttga aaatagtttc cgtctgatac ggtgaggcct caataatctg atagccattc
     3781 gattctaagg cacaggaagt atctttaata tactgaccta aatcttgaat aaatccatcc
     3841 cacagggctt tgtatttttc cacttttgat gtagggaaat accactcttt tcgggcatca
     3901 cgatcataga aaaatgccaa atcatctgct gttctaacag aaagctccat cgtattacaa
     3961 tgcgaatagc ggtacccaaa actatttcgt atgatttcaa aatccacgcc atagtgaggc
     4021 tcccaagatt caatgtgatt cagcagatta gaaaacattt tttgtgtgcg tttctgagga
     4081 tttagcttgt tgaaaagatt aattaacgtc tccgtattaa gcctaccgta gaatgctacg
     4141 ccatcgcctt gacaatgaga aagtgaatac tcaatgttta agcaattagg aaaaccagct
     4201 ttaacaagag cgtgtttaaa ataagtgttt aagactgagc cagacattgc gcttctccta
     4261 aaattagagg gaaacacatt acccttttcc ggaaattgtg tttcccgaaa gggtgaaaga
     4321 ttgattacca cttatcgaag ttaatcgctc tgaatttata aacggatata ggttgatcat
     4381 agagttctgt ttccgaaatc caattgtgtt caaccaagat ttctctgaca agatacataa
     4441 atgtactcat caaatgacga ctatgactaa acccgtttcc accaacataa gcaaaccctc
     4501 tcagggtttt gcgaattcct gctttaacaa caaggtaagt aatatcaacc tcgccataag
     4561 acgggcagac atcaaagcga gaaaaaacag aaaatgcttt gccaaacttg gtctcacgct
     4621 ctaatttcac actgaataga tcatgtgcat ggcgtaaatt ccaaagtaca ctgtataagt
     4681 gcaaatctgt ttcggtatat tgaatttttt tacgagccat aatgtttctc ctaaaaaata
     4741 aaagggaaac attacttccc tctggggata atgcctcccc ttgtgaggta taagaatagg
     4801 atgtgaatat gaagttgcct atgctcgact aacaattagc ttgccgttct gctcggacac
     4861 actcagaaag cgaaaattaa gtccttgccg agacggcatt tgttgcataa atcgcacgct
     4921 tttgatgtcc ttatctcgat tttgccacgc ttctaaagct ctatgactaa tttcttgctg
     4981 taaggcttga tgctctgatg tccattcatt aagtttagtt ggtaaaacat ctccaataca
     5041 ggaaagtcta atatgaacta acatacattt ctccagaata aggggaaatg cattcccctt
     5101 aagggaaaca acatttcccc gatgggtgag ttaaattaga ttaaagagag ctgagtacca
     5161 aaaacggcag ccataccaca tacaaaggca ataatggcag ccaacaaagt ccagaaaaac
     5221 atcttacgct taatgcggga aatttcctgt tcatattcgg ctgcttgttc gtctaagcac
     5281 atttgcagtt ctaaggctcg catttcatct tcttcagcaa tccgttgatt gatgcctttg
     5341 gtgacttcaa catacttgct tttggcttta tgtttcgctg aaacaaaaat attacctagc
     5401 ttgttgccaa gtttccaaaa aagagaaggt tttacggttt tggatgattc aaacgggata
     5461 acatcccatc ctgcggtttg ttgactcata gcgagttctc ctaaaagtta agtgaataaa
     5521 attcctcagg agatcgcaat tccaagggaa ttgaaatatc cctcggaagg taggtgtgta
     5581 ggtagcattc ctgcaaattt gcagagttcc ctttcagaat gctgggagac attttgggta
     5641 aataactcaa ctacctctaa ggatagccgc cgcttttcaa gagttatgcg acttttaagg
     5701 gctgtatcta cagcgtctta atgcttaata tcatacgaat tcctccaata aaatcaaccg
     5761 tttttttgtt caaaaaatat ctcttttttg aaaagagttt ttcctatcct caacataaaa
     5821 atctcgctaa ttttctgctt atttgttcgg gaggagagcg tgatgaaagt aagtagattt
     5881 ctgggatatt gcagtatagt aacaggtgtg ttttcgcccc ttgcacaagc acaacccaat
     5941 atcatcgtct ataccacaaa gatgtaccct attcaaaatt cacaacttgc ttcccagatt
     6001 tattatcttg accaagtaga gaattttgaa gagtcaatca gtcgaaacct cagtaaatca
     6061 ccagagattg ccgaacaaga aatgcgtgca ttctttaatt cagcagattg gaaagcccaa
     6121 gagcaacaat taaaagacgc ttatcaaggc gtgatcagtg gatggcaaaa tggcattaag
     6181 aaagtacctg cggtgttttt tgataatcca caatcttctc cgagtgtgat ttatggtacg
     6241 actgacgtac agaaagcatt agatgaatgg cataaatggg ttaaacaaca aggtgaatca
     6301 aaatgaaaaa aacgtttctt acgatactga tctctgccag tttagctgtc ccaacaacct
     6361 tatatcctca accagccaga gcctcattga ataccgcttc cattgctgca tcaagtgcct
     6421 caacaagctg tttggaatat aaagttgttg gaacctgttt ttggttattt tgtacctact
     6481 ttggatgtaa ggttcgcaca tcaaccaaaa ttaagcatta tttacctgaa atggttgttt
     6541 catcttataa ccacgatggg caaacgccat ggacagaatt acaagctctc aatcctggtg
     6601 taaaaggtgg agaataccgt tctccacaac gccaatactc tcaactcact ttcaaaaatg
     6661 cggatgcgtt tggacaccca caaggagcta tcggtcaatt attaggaaat acggggtact
     6721 attgtaaaac tcaaactacg ccatttatgc cttacttttt gagtgggctg gatttttttg
     6781 cctggcgtga aaatatgccg gaaatgtttt acccggaagc tctgactccg ggaatgcggg
     6841 aagttggccg tgtaggcgat agctggggaa atatttaccc acgttccggt tgggtaaccc
     6901 aagcacacga ttataaagcc tctgcgacca ttgtgcagag agtggcagac attgtgagcc
     6961 gaacagctca gccacacatt tatttcccag cagccaaaac aggtggtgca ggagaatggc
     7021 ctccaggacc ggtcgaagag ggacaagcaa gaacacataa atggcaaata ctttaccctc
     7081 aaatgtcaaa tacctgctct gtttttccta atggagggca aacagatacc tattcttctc
     7141 aaatttcaaa tcaaggtgat tatgcttggg cattatggcg accttatagt tgttgcaaac
     7201 gccgtgggca aacattcctt tacagtattg attggtcaca ctaataacat tgggagcaat
     7261 aatgaaatct cactttaact tcaaaaaatc ccacttacgg atcacgacag cattattatc
     7321 aagtgtatca ggactatctt atgccgattc gattacggat aaactggaat ttagtaagtc
     7381 ggattcgatt atttcagacc gtgtttttta ccaaatcggc ggtggaatgg gttatatggc
     7441 tccacctagt cgtggtaata ccaaagttgt tgatttagga attggttgga aagcgaattt
     7501 aacctgtggt aattttgata ttaaaacgac cattaaaaat gatttaaacg gtctcaaaga
     7561 cggctttaaa gatttaatgg ggaatgtcat tgattcagcg aaaggtgcgg ttgcaagtat
     7621 gcctgcaatg gtattgcaac gggccaaccc tcaactctac gatttactca caaatggtat
     7681 gtaccaaggt aagctggatt tcaaccgtct taaaaccagt tgtgaagaaa tgtccaaagt
     7741 tttagcagat catacactgg gaggaaaatg ggggcaagca gctgatttag aaagctataa
     7801 gcaaattacc tcctctcaac ctgatgcaaa acaagctcaa aaagacctgg aagataaaaa
     7861 agggaaagat gggcgagttt ggataggagg tcagaaaaaa ggtggggaaa accaagaagc
     7921 tatcaatgtt gttggcgatg ttgtcaaagc tgggtttaat ttactgaaca aacgtaatgc
     7981 cttagatacc agtacaattt caggttcaag ctgtgatggg aatctttgcc aaacttggga
     8041 taatccacaa acagcagccg attttttaac taagattatt ggtgaacaat ctatttcgac
     8101 ttgtaaagag gattgtggca ctaaaaccag ctcaaaagcc ggtgtaggct tgagtccgga
     8161 aattgaggaa gaacatgttc gtactattgt caaattagag aatgtactga atatggatgt
     8221 gcctaaggct gaattgttag ctgatttgag ttcaactact gtaccagtaa caagagggct
     8281 tattgagagt cttcgagatg atcctgatgt tgaggtatta agccaacgct tatctgcaga
     8341 aattgcgact agtaagataa tggaaaaaat gctcctggct cggcgagcag tcttagctgg
     8401 tatgcgtgag ccaaatgtcg ctcaaaatgc ggatgcacaa gtagaactgg aaagagtact
     8461 caatattatt gaccgtgaaa tttcccaagt aaaactagaa atggatatgc aaaaaatgat
     8521 caccggcaat acggcttcgg tgattttgca aagcaaatcc gaacgagaag ctcttgctgg
     8581 tgctcatagt ttatctcaag acagtctgga taaacgtgtt caaggacttg aaaacggtaa
     8641 tgttgcatta actgatttag aggcaggctc taatattaac ctctctcgta gaaatattgc
     8701 attgagtatc ccgaatattc cgaatgtagc tgctctcata cctaatctgc caaatcgagg
     8761 tggttcaggt gtaggtggaa atttcccatc actcgcacaa atcagcggtt caacgatgga
     8821 tcaagcaaca ggattactac gtaattttga aggctttatc tctaaggctc aatgggatgt
     8881 aaatgctcat cgagtaggat atggttccga tacaattacc cgagctgatg gtacgatcgt
     8941 aaaagtaacc aaagatacgg tcattaccaa agaagatgca gagcgtgatt tggcccgccg
     9001 aacagctatc ttcgcaaata atgtgagaaa agagcttgga gattcaaatt ggaatgcgtt
     9061 accgccgaac gctcaagctg ttttagtgtc ttatgcatat aattatggtt cacttgctaa
     9121 aacaaaaagc gtacttgatg cagccagaag atcagctcaa agtggtgata tgaatgcttt
     9181 agctactgca atcagaaatc gccaagttga taataatggc atcaatgcac gtcgccggaa
     9241 tcaggaagct gactatattc ttggtaaaaa ttaacctcat aaggagttac atttatgcgt
     9301 aaattattag gtattactgt acttacactt tgtacaactt cagcatttgc aacggataaa
     9361 aatgtggtat tttcttgtac cagcacagaa ggtaaacctc tcacagttaa acgggtagga
     9421 aatgattacg agtattcgta tgataaaact atcttcaaaa acccaatcaa aaaagcggtg
     9481 accaacgatg gctcaattat cgctagagga tcaggtttta ccacgtatgc attagaactg
     9541 aaaaatgatg gtttgaagta tgtagttggg tttgttcaac caaatggtaa ctcaaaagag
     9601 tttattgagc ctggagcaac gattagccgt gataatgatc aaccaagcat tggctctgta
     9661 gattgtgatc cttacaaaaa aatctattat aagtttgatg ttcatttaat gaataccttg
     9721 taatgcgata attcaataat atgggggaat ggcttgattt ttcatccccc atactctatt
     9781 atatagatgt ggtgctagag ccagcccacg ttaaaaaata caaagctcgg tagatgaact
     9841 attgtggtgc cagagccggc ccacgttaaa aaatacaaag ctcggtagat gaggcccctt
     9901 aagtcgtaag atttaagggg ttttgcttag gatataaaat ggatattaca acacaaaata
     9961 ttgaacttag atacttatct gagcagtttt accttgatgt cgctccacat ctgaaagaaa
    10021 ttttgcttaa agagactcgc ccatactgtg ttctattagt tagagttaaa aatttagatt
    10081 ttgccctacc attgcgttct aatttacctg aaagagaagg agttggaatt aaaacgatac
    10141 ctaatcctca ggaatacggc aaatttaaag gaattgattt ctcaaaagcc attctactga
    10201 cagataaaag ctatttaaaa acagataccg ttcagctcaa aaacaaatca gaattgttga
    10261 atatccaagg taatgagcgt agaattgttc gtgaatttaa gaaata
//
LOCUS       DANLYI010000075        10169 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.075, whole genome shotgun sequence.
ACCESSION   DANLYI010000075 DANLYI010000000
VERSION     DANLYI010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 10169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10169
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.075"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            36..257
                     /locus_tag="PXD81_001353"
     CDS             36..257
                     /locus_tag="PXD81_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005607413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1414 domain-containing protein"
                     /protein_id="HDL5537374.1"
                     /translation="MATKSKYHTKQFEQLLADLIATIEKHKAPVDLSLMALGNMITNI
                     LIENIQSPAQREALADAFADALKQSLKTK"
     gene            262..1995
                     /locus_tag="PXD81_001354"
     CDS             262..1995
                     /locus_tag="PXD81_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005607412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3413 domain-containing protein"
                     /protein_id="HDL5537375.1"
                     /translation="MFARLRSLLPTNSRQYREETSQKITWGHWFALFNIVLALLISSR
                     YAFNADWPNTLVGKLYFFVSLFGHFSFVVFAGFLLILFPLSFIVKNHRTYRGLSVILA
                     TIGQTVLLVDTEVFKQFYLHLSPLVWDLLVNPEEGELTRKWQLLFVPMPLLLLAEMLY
                     SRWCWQKLRSFSRQKWGKYVALFFVISFTATHLIYAWADMNAYRPITAQRANYPLSYP
                     MTARNFLEKHGFIDRVEFNQEIEESGRLDTFYLNYPKSPLIATKTPSTNILLINLSGL
                     SKQIINAETMPKLTEISQQSHRFMQHYSSGDSASAGALGLFYSLSGKYLDSVLADKTV
                     SPLIEIARKSDYQLGLFSHNGFADPIYHQAIFSGILLPTAHSNVGAIGQWKNWISSRS
                     LENPFFSYLDLRMPFAHTAQEQAKLLDWQFAEIWQHLTQNALLANTLVIITADISNEV
                     GAENSFDKQHIQVPMMFYWQGESKEYHFLSSHLDIMPTLLSQFFGVQNPLNDYAQGMD
                     LSTQNQRLWVSSSNHKWNVAIMPNGDQYQIDRKGNFKHFNSNGEHEKDARPPLALFLQ
                     MIQQSNQFIEK"
     gene            complement(2062..2763)
                     /locus_tag="PXD81_001355"
     CDS             complement(2062..2763)
                     /locus_tag="PXD81_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL5537376.1"
                     /translation="MIGQALGWRATFGIIGIIALIVLMMIWRLLPYLPSKNAGSIKSL
                     PLLFKRPMLIGIYLLVALIVSGHFTAYSYIEPFMIKISSVSVENTTFILLVFGLAGVV
                     ASFLFGRLYGKAPNKFILSGIAIIMCSQLCLLFLSDSTVAMFMVAFVWGIGITGMSIA
                     LQMKVLELAPDATDVATAIFSGTYNLGIGGGALLGSIAIQQLGLANIGFAGGSIAFVA
                     MVWFVFVSLKYRQLR"
     gene            complement(2760..3140)
                     /locus_tag="PXD81_001356"
     CDS             complement(2760..3140)
                     /locus_tag="PXD81_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL5537377.1"
                     /translation="METLAIFLFSEIWKFDFVFTFIFKILKINVFFSDLINMKLARKA
                     QRRLSFLRVFSLAFAAFIVNTTEFIPIALLSDIASSFQMEPSSAGIMMTVYAWVVFLL
                     SLPLMLLTANMERKRLLLMLFMGE"
     gene            complement(3182..7642)
                     /gene="mukB"
                     /locus_tag="PXD81_001357"
     CDS             complement(3182..7642)
                     /gene="mukB"
                     /locus_tag="PXD81_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HDL5537378.1"
                     /translation="MIDTTELQNNPQIAPLARPNDVQRGKFLSLTLINWNGFFARTFD
                     LDDLVTTLSGGNGAGKSTTMAGFVTALIPDLTLLNFRNTTEAGSTAGSRDKGLYGKLK
                     AGVCYAVLESFNSRGQRIITGVRLQQIVGRDKKVDIRSFSLQNIPQEQSIISLLTEQV
                     GNKARVLALNELKDQFENSEVQFKQYHSITDYHSFMFDLGVIPKRLRSAADRSKFYKL
                     IEASLYGGISSVITKSLRDYLLPENTGVRQAFQDMESALRENRMTLEAIKVTQSDRDM
                     FKRLITESTNYVSADYMRNANERRGNVQQALEQRKEWYAAKSKILLEQQRFVEFSRES
                     ADIAEAELALEADYNSANDHLNLVMNALRHQEKIERYQDEVEELNIKLEEQQEALEEI
                     AEIAENAQARADEADDHVEELRSQMADYQQALDAQQTRALQYQQAVNALEKAKQLTGL
                     VNLDLNNIEDYHAEFVAQAEDLTDQVFELEQRLSVSDMAKTQFEKAFELVCKISGEID
                     RLQAWEEARALLSAFPEQKMQAQQAVSLRQKLNDLEQRLQQQQNAQRLVAEFNQKSQQ
                     QLNSAEELDAYFEEQQARLEDLEAELSDFVEQRSTQRQQREQLNQQYQQLAKNAPAWH
                     TAQSALTRLEEQCGEKFEASQSVMQFMQNMLSKEREATLARDELARKEQMLDEQINRL
                     SQPDGSEDARLNQLAERFGGVLLSELYDDVSIEDAPYFSALYGEARHAIVVRDLEAVK
                     TQLEKLDDCPSDLYLIEGDPSAFDDAVFSAEELGEGVVVKVSDRQWRYSKFPEVPLFG
                     RAAREKHLATLKAERDETAEKHAERAFDVQKCQRLHQHLSQFIGTHLNLAFQENPEML
                     MQEIAAERAEIERELAQSAGNEQQLRHQLESVKAQLQMLNKILPLANLLADETLEARA
                     EECREQLELAEEDEMFIRQFGATLSQLEPIAAALKSDPALFEQLEADYRRSVAEQKVL
                     QQKVFSLADVMNRRLHFSYQETVGTEGSALTEQLRQRLENAQREREQARDQLRQAQAQ
                     LSQYNQVLTGLRSSYEAKNQMLQELIREIDDLGVRGDSGAEERARFRRDELQQRLSQQ
                     RSRKGYLDKQIAVIEAEMDNLNRTLRKAERDYHTQRELVVQAKASWCIVQRLSRNSDV
                     EKRLNRRELAYQSAEELRSISDKALGALRTAVADNEYLRDSLRASEDSRKPENKVAFF
                     IAVYQHLRERIRQDIIKTDDPIDAIEQMEIELSRLTNELTGREKKLAISAESVANILR
                     KTIQREQQRILQLNQGLQNIAFGQVKGVRLVVNIRDTHAILLNALSNNREEHKDLFDS
                     QKLSFSEALAMLYKRVNPHIEMGQRTPQTIGEELLDYRNYLDLEVETFRGADGWMRAE
                     SSALSTGEAIGTGMSILLMVVQSWEEEARRMRAKDILPARLLFLDEAARLDATSINTL
                     FELCERLDMQLLIAAPENISPERGTTYKLVRKITNNQEYVHVVGLKGFAK"
     gene            complement(7818..8534)
                     /gene="mukE"
                     /locus_tag="PXD81_001358"
     CDS             complement(7818..8534)
                     /gene="mukE"
                     /locus_tag="PXD81_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HDL5537379.1"
                     /translation="MTENIQDFISPKLALAIANPIFPELDSQLRAGRHINTEQLDQHV
                     FLMDFQAELESFYRRYHVELIRAPEGFFYLRPKASTLIARSAMSEMEMLVGKVLCYLY
                     LSPERLAQQGIFSQDDVYEELLSLADESKLLKAVNPRSTGSDLDRAKLAEKAGGALRR
                     LARIGIITRVGEQNSKKFVISESVFRFGADVRTGDDPREAQLRLIRDGEATTPALLNE
                     QAVEIDENIADEAPFDGENE"
     gene            complement(8583..9926)
                     /gene="mukF"
                     /locus_tag="PXD81_001359"
     CDS             complement(8583..9926)
                     /gene="mukF"
                     /locus_tag="PXD81_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011848432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HDL5537380.1"
                     /translation="MQNELAQTIPELISWTKDREFSLSLSSDRLAFLLAISIYNQEQT
                     DGELLESDLIDLFRYVSNSFEQSDATFTQRANNAINDLVRQRLLNRFSSEFTEGLAIY
                     RVTPLGVGISNYYVRQREFSTLRLSIQLSIVADEIQRASVAAEEGIEQAQDERFWRNN
                     VFAPLKYSVAEIFDSIDLSQRIMDENQHQIRERIAELLSQNWHEAIMSCEQLLDETSG
                     NLRELQDTLNAAGDKLQAQLLRIQSCLMARNDLDFIDQLIVNLQNKLDRIISWGQQAI
                     DLWIGYDRHVHKFIRTAIDMDKNRVFGQRLRQSIQDYYNSPWLLYTAKADSLLDLRDD
                     EAMLNEAESVGELPSELEYESLSDVQEQIVSAMQAHLAPFRAEGKPIDLGVILREQLA
                     RFPESRHFDVARIIVDQAVKLGMASQDSQAVYPQWQPINEQGAEVQANVIDQYNK"
BASE COUNT         2842 a         2372 c         2005 g         2950 t
ORIGIN      
        1 tttgtaccta attataggat catctttatt tcaatatggc aacgaaatct aaataccaca
       61 ccaaacaatt tgagcagcta ttggctgacc tcatcgccac catagaaaag cataaagcac
      121 cggtggattt atcgttaatg gcattaggca atatgataac taacatttta attgaaaata
      181 ttcaaagccc ggcacaaaga gaagctctcg ctgatgcctt tgccgatgca ttaaaacaat
      241 cactaaaaac caaataagat gatgtttgca cgcctacgca gtttattgcc gaccaattct
      301 cgccaatatc gagaagaaac ctcacagaaa atcacgtggg ggcattggtt tgcactattt
      361 aatattgtgt tggcactact catttcctct cgttacgcat ttaatgcaga ctggccgaac
      421 acgcttgtag gcaaacttta tttctttgtc agcctgttcg ggcatttcag ctttgtggtc
      481 tttgcaggat ttctattaat tctgtttccg ctcagtttta ttgttaagaa tcatcgcacc
      541 tatcgtgggc tttccgtgat tttagccacc atcgggcaaa ccgtgttatt ggtggatact
      601 gaagtcttca agcaatttta tctgcattta tcaccgcttg tatgggatct gttagttaat
      661 ccggaagaag gggaactgac ccgtaaatgg caactgctgt ttgtaccaat gccactcctt
      721 ttgctggcag aaatgctcta ctctcgctgg tgctggcaga aactgcgtag ttttagccgc
      781 caaaaatggg gcaaatatgt cgctctcttt ttcgtgatca gctttaccgc tactcattta
      841 atctatgcgt gggcagatat gaatgcctac cgcccgatta ccgctcaacg agcaaactac
      901 ccactttctt acccgatgac ggctcgtaat ttcttagaaa aacacggttt tattgaccga
      961 gtagaattta atcaagaaat tgaagaaagt ggcagactag atactttcta tctgaattat
     1021 ccaaaatctc ccttgattgc gaccaaaacg ccctcaacta atattttact gattaattta
     1081 tcaggtttat ctaaacagat aataaatgcc gaaacaatgc ctaagctgac agaaatcagc
     1141 caacaaagcc accgctttat gcagcattac agtagtggtg attcagcctc tgccggtgca
     1201 ttaggtctat tttacagttt aagcggtaaa tatttggatt ccgttttagc cgataaaacc
     1261 gtgtcaccgc ttatcgaaat cgctagaaaa tcggactatc aattagggct gttttcacat
     1321 aatggttttg ccgacccgat ttatcatcag gcgatcttct ctggtatttt attgcctacg
     1381 gcgcacagta atgtaggggc gattgggcaa tggaaaaact ggatcagctc tcgctcattg
     1441 gaaaatccgt tctttagcta tttagactta agaatgccct tcgcccacac cgctcaagag
     1501 caggcaaaat tattagattg gcaattcgct gaaatctggc aacatttgac ccaaaatgca
     1561 ctattggcga atacgctggt aatcattacc gccgatatta gtaatgaagt tggagcagaa
     1621 aatagcttcg ataaacaaca cattcaagtg ccgatgatgt tctactggca aggcgaaagc
     1681 aaagagtacc atttcctttc aagccattta gatattatgc caaccttact gagccaattc
     1741 ttcggcgtgc aaaatccgct gaatgattat gcacaaggta tggatttaag cacccagaac
     1801 caacgtttat gggtatccag ttctaatcac aaatggaatg tggcgattat gccgaatggc
     1861 gatcaatatc aaattgatcg taaaggcaac tttaagcact ttaacagcaa tggcgaacac
     1921 gagaaagatg ctcgaccacc attagcgttg ttcttacaaa tgattcaaca aagcaatcaa
     1981 tttattgaaa agtaatgttc aacaagcggt catttttgca ggaaaatttg caaaatttca
     2041 gcaaaatatg accgcttgtc actatctgag ctgtctatat ttcaagctaa caaatacaaa
     2101 ccaaaccatt gctacaaaag caatactccc accggcaaat ccgatattgg ctaatcccag
     2161 ttgctgaata gcgatgctcc ccagtaatgc tccaccgcca atgcctaaat tataagtgcc
     2221 ggaaaaaatt gcagttgcga catcagtcgc atcaggggca agttcaagca ccttcatttg
     2281 caacgcaatg ctcattccgg taatgccaat tccccagaca aaagcgacca taaacattgc
     2341 cactgttgag tcactcaaaa acagtaaaca aagctggctg cacataataa tggcgatccc
     2401 gcttaagata aatttattcg gggctttgcc atataaccga ccaaataaaa aacttgccac
     2461 cacacctgcc aagccaaaaa ccagcaaaat aaaggtggtg ttttccacac tcaccgaact
     2521 gattttaatc ataaacggct caatatagct gtaagcggta aaatgtcctg aaacgatcaa
     2581 tgccaccagc aaataaatgc cgattaacat cgggcgttta aataacaaag gcaaactttt
     2641 tatcgagcct gcatttttgc ttggtaaata aggcagcaac cgccaaatca tcattagcac
     2701 aatcaaagcg ataatcccaa taatgccaaa ggttgcccgc cagcctaagg cttgcccgat
     2761 cattcgccca taaataacat cagcaacagc cgttttcttt ccatattcgc cgtgagtaac
     2821 atcaacggta acgataataa aaataccacc cacgcataaa ccgtcatcat aatgcctgcc
     2881 gatgaaggct ccatttggaa actgcttgca atgtctgaaa gcaatgcaat aggaataaat
     2941 tcggtggtgt ttactataaa agccgcaaag gcaagactaa atactctaag gaacgataat
     3001 ctccgttgag cttttcgagc aagtttcata ttaattaaat ccgagaaaaa cacatttatt
     3061 ttcaaaattt taaaaataaa tgtaaaaaca aaatcaaatt tccaaatttc tgaaaataaa
     3121 aagattgcca atgtttccat caacaatctt tttatacata tttagattaa agtgctaagt
     3181 tttatttagc aaagcctttt aaaccaacaa cgtgaacata ctcttggtta ttggtgattt
     3241 tacgcaccaa tttataagtt gtgcctcgtt cagggctaat attttcaggg gctgcaatta
     3301 ataactgcat atccaaacgt tcgcagagtt caaatagcgt attaattgag gtagcatcta
     3361 atcgagccgc ttcatctaag aacaataagc gagccggtag aatatctttg gcacgcatac
     3421 ggcgagcttc ttcttcccag ctttgaacta ccattaagag aatcgacata cctgtaccga
     3481 tcgcctcacc ggtcgataat gcactacttt ctgcacgcat ccagccgtct gcaccacgga
     3541 aggtttccac ttctaaatct aagtagttac ggtaatccaa taactcttca ccaatggtct
     3601 gtggtgtacg ttgccccatc tcaatatgag ggttcacccg tttatacaac atcgctaagg
     3661 cttcggagaa gctcagtttt tggctgtcga ataaatcttt gtgttcctca cgattattgg
     3721 ataacgcatt gagtaaaata gcgtgcgtat ctcggatatt caccaccaaa cgcacccctt
     3781 ttacctgacc gaatgcaata ttttgtaaac cttggttcag ttgcaaaatg cgttgttgtt
     3841 cacgctgaat ggttttacgt aaaatatttg ccacactttc cgcactgatc gccagttttt
     3901 tctcacgacc ggttaattca ttggttaagc gtgaaagctc gatttccatc tgctcaatcg
     3961 catcaatcgg atcatcggtt ttgataatgt cttggcgaat ccgctcacgc aaatgctgat
     4021 aaaccgcaat aaagaacgcc actttgtttt ccggcttgcg actatcttca gaagcccgca
     4081 agctatcacg caggtattca ttatccgcca cggcggtgcg taatgccccg agagctttat
     4141 ccgaaatcga acgtaattct tcagccgatt gataagccaa ttcacggcga tttaaacgtt
     4201 tttccacatc gctgttacga gataaacgtt gcacaataca ccagctggct tttgcctgca
     4261 ccaccaattc acgttgtgta tgataatcac gttcggcttt acgtaaggtg cggttaaggt
     4321 tatccatttc cgcctcgatc accgcaattt gtttatctaa ataacctttg cgggaacgtt
     4381 gctggcttaa acgttgttgc aattcatcac ggcggaatct ggctcgctct tccgctccgc
     4441 tatcaccacg cacgcccaaa tcgtcaattt cacgaattaa ctcttgtagc atttggtttt
     4501 tcgcctcata agaactgcgt aagccggtca atacttggtt atattgtgaa agttgagctt
     4561 gagcttgacg cagctgatca cgagcctgtt cacgctcacg ttgtgcattt tccaaacgtt
     4621 ggcgtagctg ttcggttaat gcagagcctt ctgtacccac cgtttcttga tagctaaaat
     4681 ggaggcggcg attcattaca tcagccaagc tgaacacttt ttgctgtaac actttttgct
     4741 cggcaactga acggcggtaa tcggcttcca actgttcaaa taaagccgga tcacttttta
     4801 atgcggctgc aatcggctca agttggctta atgttgctcc aaattggcgg ataaacattt
     4861 catcttcttc tgccaattct aattgctcac gacattcctc cgctcgtgct tccagcgttt
     4921 catcggctaa taaattcgca agcggtagaa ttttattcaa catttgcaac tgggctttta
     4981 cgctttctaa ttggtgacgt aattgctgtt cgttaccggc tgattgtgcc aattcacgtt
     5041 caatttctgc ccgttctgcc gcaatctctt gcattaacat ttcagggttt tcttggaacg
     5101 ctaaatttaa gtgcgtgccg ataaattggc ttaagtgttg atgtaaacgc tggcatttct
     5161 gcacatcaaa ggcacgttca gcgtgttttt ctgcggtttc atcacgctct gcttttaaag
     5221 tcgctaaatg tttttcacga gcagcacgtc cgaataacgg cacttcaggg aatttagaat
     5281 aacgccattg gcgatcggag actttcacca ccacgccttc gcctaattcc tcggcactaa
     5341 ataccgcatc gtcaaaggcg gacggatcac cttcaattaa ataaagatca cttgggcaat
     5401 cgtctaattt ctctagctgt gttttaaccg cctctaaatc acgcactaca atagcgtggc
     5461 gagcctcgcc gtataaggcg gagaaataag gcgcatcttc aattgataca tcatcatata
     5521 actcagagag taacactcca ccaaaacgtt ctgccaactg gtttaagcgg gcgtcttccg
     5581 agccgtccgg ctggcttaag cgattaattt gctcatcaag catttgttct ttacgagcca
     5641 attcatcacg agcaagggtg gcttcacgct ctttgctcag catattttgc ataaactgca
     5701 tcactgattg gctggcttca aacttctcgc cacattgctc ttctaaacga gtcagagccg
     5761 attgtgccgt atgccaagcc ggtgcattct tcgctaattg ctgatattgt tgattaagct
     5821 gttcacgttg ttggcgttgg gttgaacgtt gttctacaaa gtccgaaagc tctgcctcta
     5881 aatcttctag gcgagcttgc tgctcttcaa aataggcgtc taattcttcc gctgaattga
     5941 gttgttgttg cgatttttgg ttaaattccg ccactaaacg ctgggcattt tgctgttgtt
     6001 gcaaacgctg ttctaaatcg ttgagttttt gacgtaaact caccgcttgc tgagcttgca
     6061 ttttttgctc agggaaggca gaaagtaacg cacgagcctc ttcccacgct tgcaagcggt
     6121 caatttcacc ggaaattttg caaaccaatt caaacgcttt ttcaaattgg gttttcgcca
     6181 tatccgacac gcttaaacgt tgttccagct caaatacttg atcggtaaga tcttcggctt
     6241 gagccacaaa ttctgcgtgg taatcttcaa tattatttaa atctaaattc actaagccgg
     6301 ttagctgttt ggctttttct aacgcattca ccgcttgttg gtattgcaat gcacgagttt
     6361 gctgtgcatc taaggcttgt tggtaatccg ccatttgaga acgcaattct tcaacgtgat
     6421 catctgcctc atccgcacga gcctgagcat tttcggcaat ttccgcaatt tcttccaatg
     6481 cctcttgctg ttcttccagc ttaatattca gctcttcaac ttcgtcttgg tagcgttcga
     6541 ttttctcttg gtggcgtaac gcattcatca ctaaatttag atgatcgttc gcactattgt
     6601 aatccgcctc taacgccagc tctgcttcgg caatatccgc actttcacgg ctaaattcca
     6661 caaagcgttg ctgttcgagc aaaattttgg attttgccgc ataccactct ttgcgttgct
     6721 ctaaggcttg ttgtacattg ccacggcgtt cattcgcatt acgcatataa tccgccgaca
     6781 cataattggt cgattcggta attaagcgtt tgaacatatc ccgatccgat tgcgtgacct
     6841 taatcgcctc taaggtcata cggttttcac gcagagcgga ttccatatct tggaacgctt
     6901 ggcgaacgcc cgtattttcc ggcaacaagt aatcacgcaa ggatttggta atcacacttg
     6961 aaataccacc ataaagcgag gcttcgatta atttgtagaa tttgctacgg tcggctgccg
     7021 aacgaaggcg ttttgggatt acgcccaaat cgaacataaa actgtggtaa tcagtaatcg
     7081 aatggtactg tttgaattgg acttcgctgt tctcaaattg atctttgagt tcatttaacg
     7141 ccagcactcg agccttattc cccacttgtt cggtcagtag gctgataatg ctttgctctt
     7201 gaggaatatt ctggagcgag aacgaacgaa tatcgacttt tttatcccgt ccgacaattt
     7261 gttgcaaacg cacgccggta ataatccgtt gcccacggga attgaatgac tctaacaccg
     7321 catagcaaac acccgctttt aacttaccgt ataagccttt atcacgagaa ccggcggttg
     7381 agcctgcttc agtcgtatta cggaaattaa gcaaggttaa atctgggatc aaggcggtca
     7441 caaaccctgc cattgtggtg gacttacctg ccccgttacc gcctgaaagc gtggtcacta
     7501 agtcatctaa atcaaaggtt cgagcaaaaa aaccgttcca gttaatcagc gttaaagaga
     7561 gaaatttacc gcgttgtaca tcattcgggc gtgcaagcgg tgcaatttga ggattatttt
     7621 gcaattctgt ggtatcaatc attatgtttt ctcactaatt gtcctagcac gctaaagcgt
     7681 actaggttag gtataggtga gtagctccgc tactctaatc gcaacctcag ccttggaggg
     7741 ctgctttatg cttaacctag ttcggcttta ccgtgtctag ggcagactca ttaaatgctg
     7801 aatctattcg aatgaaatca ctcattttct ccgtcaaagg gggcttcatc tgcaatattt
     7861 tcatctattt caaccgcttg ttcgtttaac aacgcagggg ttgtcgcttc accatcacga
     7921 attaagcgaa gctgtgcctc tcttgggtca tcaccggtac gcacatcagc cccaaaacgg
     7981 aagaccgatt ctgaaatcac aaattttttg ctgttttgct cgcctactcg ggtaataatg
     8041 ccgatacggg ctaatctgcg taacgctccg ccggcttttt ctgccagctt ggcacggtct
     8101 aaatcggaac cggtcgaacg tgggtttacc gcttttaata atttgctttc atcggcaaga
     8161 ctgagtaatt cttcatacac atcatcttgg ctgaaaatcc cctgctgagc caaacgttcc
     8221 ggacttaaat agaggtagca caacacctta cccactaaca tttccatttc tgacattgcc
     8281 gaacgggcaa ttagtgtcga ggctttcggg cgtaggtaga aaaagccttc cggtgctcga
     8341 atcaactcaa cgtgataacg gcggtaaaaa ctctctagtt ccgcttgaaa atccatcaaa
     8401 aagacgtgtt ggtcaagctg ctcggtattg atatggcgac cggctcgtaa ttggctgtct
     8461 agctccggaa aaatcgggtt ggcaatcgca agtgccagtt tcggggaaat gaaatcttgg
     8521 atattctctg tcattgtcat tctcttcaat tttgcaaaat tctgggtaaa aacgaccgct
     8581 tgttatttat tgtattggtc gataacattc gcctgcactt ccgccccttg ctcgttaatc
     8641 ggctgccatt gtgggtaaac ggcttggcta tcttggcttg ccatacctaa cttaacagct
     8701 tgatcgacaa taatacgagc cacgtcaaaa tggcgggatt ccgggaatcg ggcaagctgt
     8761 tcacgcaaga taacgcctaa atctatcggt ttaccttcag cacggaacgg cgctaaatga
     8821 gcttgcattg ccgaaacaat ttgctcttgc acatctgaaa gcgactcata ctctaactca
     8881 cttggcaact cgcccaccga ttctgcctca tttaacattg cttcatcatc acgcaagtcc
     8941 agtaacgaat cggcttttgc agtataaagc aaccaagggc tgttgtaata atcttgaatc
     9001 gattggcgta accgttgccc gaatacacgg tttttgtcca tatcaatcgc tgtacgaata
     9061 aatttatgca catggcggtc atagccgatc caaagatcga tagcttgttg tccccagctg
     9121 ataatacggt ctaacttatt ttgtagattc acaatcagct gatcgataaa atccaaatca
     9181 ttacgagcca tcaggcaact ttgaatccgc aataattgag cttgcagttt atcaccagcg
     9241 gcatttaagg tatcttgcaa ctcacgcaaa ttccccgagg tttcgtctaa caattgctca
     9301 caactcataa ttgcttcgtg ccagttttgg ctaagcagtt cggctattcg ttcacggatt
     9361 tggtgctgat tttcgtccat aatccgttgt gaaagatcga tgctgtcaaa gatttccgcc
     9421 accgaatatt tcaatggggc aaacacatta tttcgccaaa aacgttcatc ttgtgcctgc
     9481 tctattccct cttccgctgc tactgaagct cgctgaattt catctgccac aatcgaaagt
     9541 tgaatcgata aacgcagggt agaaaattcc cgctggcgaa cgtaataatt tgaaattccc
     9601 acccctaacg gcgtaacacg ataaatcgcc aaaccttccg taaattcgct actaaaacgg
     9661 tttaacaacc gttgtctgac taaatcatta atcgcattat tggcacgctg ggtaaaagtg
     9721 gcatcagatt gctcaaacga atttgaaaca taacggaaaa gatcaataag atcactttct
     9781 aacaattcac catctgtctg ttcttggtta taaatagaga tagcaagtag aaacgctaag
     9841 cggtcggaag aaagcgaaag tgaaaattca cgatctttcg tccaagagat gagttctgga
     9901 atagtctgtg ccaattcgtt ctgcatacgg gcttaatctt taatttctat acaaaaatgg
     9961 tatttattgt aactgaatgt aatcaaaatg ggaatggata aaacgcagac aagagaaatg
    10021 aacaatataa gcggtcaaat atgggtgatt ttttgcaaaa agtattttaa aggtaaatga
    10081 atccgaaaat ttattatcac tacgctaatt tatgatacca taaatagaaa agactggatc
    10141 caatagaata tcagacacag ccccttaga
//
LOCUS       DANLYI010000076        10120 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.076, whole genome shotgun sequence.
ACCESSION   DANLYI010000076 DANLYI010000000
VERSION     DANLYI010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 10120)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10120)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10120
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.076"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            382..1734
                     /gene="lysC"
                     /locus_tag="PXD81_001360"
     CDS             382..1734
                     /gene="lysC"
                     /locus_tag="PXD81_001360"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005604206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HDL5537381.1"
                     /translation="MSHLSVAKFGGTSVANFDAMTSSANIVIADANTRVVVLSASAGV
                     TNYLVELANGCEKEHRDEVIAAVRQIQYNIIEKLQHQDAVKAEIDELLERIETLAESA
                     SLATSAALSDELISHGEMMSTKIFTQLLIERGHPAVWVDVRDVVATNSHYGKAAPNDE
                     KTQQQSDNVIKPLIASGKIVITQGFIGRDDEGKTTTLGRGGSDYSAALLAEVLNANDV
                     LIWTDVPGIYTTDPRVVPNAQRIDTMAFNEAAEMATFGAKVLHPATLLPAVRSNIPVY
                     VGSSKAPEQGGTWVTRDPQPRPTFRAIALRRNQILLTLSSLSMLHAQGFLANVFAILA
                     KHKISVDVITTSEVSVALTLDKTGSASSGADMLSAELLDELNAYCNVQVESDLALVAI
                     IGNNLHAQSGVAKKLFHTLENYNIRLISYGASSNNVCTLVKNAEADDVVRSLHKGLFE
                     "
     gene            complement(1805..4051)
                     /gene="parC"
                     /locus_tag="PXD81_001361"
     CDS             complement(1805..4051)
                     /gene="parC"
                     /locus_tag="PXD81_001361"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HDL5537382.1"
                     /translation="MTTEINYEGIEQMPIKRFTEDAYLNYSMYVIMDRALPFIGDGLK
                     PVQRRIIYAMSELGLNASAKYKKSARTVGDVLGKFHPHGDSACYEAMVLMAQPFSYRY
                     PLVDGQGNWGAPDDPKSFAAMRYTESKLSKIAEILLGELGQGTVDYQPNFDGSLEEPK
                     YLPARLPHILLNGTMGIAVGMATDIPPHNINELADASVMLLDNPKATLDDVLSVVQGP
                     DYPTEAEIITPKAEIARMYEQGRGSIKMRAVWKKEEGEIVISALPHQASPSKIIEQVA
                     TQMRNKKLPMVDDIRDESDHENPIRIVIVPRSNRIDFDALMDHLFATTDLEKSYRVNM
                     NMIGLDGKPAVKNLLTILNEWLSFRRTTVTRRLNYRLDKILNRLHILDGLMIAFLNID
                     EVIEIIRNEDEPKAELMARFNLTDVQAEAILNLRLRHLAKLEEHQLQAEKSELEKERD
                     ELQLILGSERRLNSLIKKEIQADAKAFASPRRSPLVERAESKAIAESDLTPTEDVTVI
                     LSEKGWVRCAKGHDIDVQALSHRAGDGYLAHARGRSNQPVVFLDSTGRAYALDPTSLP
                     SARSQGEPLTGKITLPEGAVVQQVLMASPETKVLMASDSGYGFICTFADLVSRNKAGK
                     AVISLTENAKVLPPQLLENDENLSLVAMSNVGRMLVFPVSELPQLSKGKGNKIINISA
                     AAAKSGDEYLARLLVIKPTNSLVFVSGKRKITLKPSDVDNYRGERARKGSQLVRGLST
                     NSTVEIVE"
     gene            4236..5273
                     /gene="pstS"
                     /locus_tag="PXD81_001362"
     CDS             4236..5273
                     /gene="pstS"
                     /locus_tag="PXD81_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005756281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HDL5537383.1"
                     /translation="MAKRFKLLTVLGISFSLSVAANAQTITGAGASFPYPIYAKWAGL
                     YEKETGNKINYQSIGSGGGQQQIIAKTIDFGASDDPMKGETLAEHKLLQFPAIIGGTV
                     PVVNIPELKSGELKLSGKVLADIFLGKITQWNDPAIVALNQDIALPAKTILVVRRSDG
                     SGTTFGWTNYLSKVSAEWKEKVGEGKSVKWPVGQGGKGNEGVAAYVKQLKYSIGYVEY
                     AYAKQNALAWVSMQNADGKFVQPSRETFAAAAANADWSQTAGMGVILTNEAGEKAWPI
                     TAASFILIHQHSDKPEQTKGVFKFFDWAFKHGKDAAAELDYVPLPDNVVEKIQTQWKT
                     EVKDGQGNSLY"
     gene            5354..6313
                     /gene="pstC"
                     /locus_tag="PXD81_001363"
     CDS             5354..6313
                     /gene="pstC"
                     /locus_tag="PXD81_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HDL5537384.1"
                     /translation="MHKHKIDNKCLNHPWVEGLFHHTTRFFALLVFALLVVVLVSLLL
                     GSWEALSRFGVSFLWENEWNPVNDRYGALVPIIGTLISAGIALVIAVPVSFGIATFLT
                     ELAPQWLKRPVGTAVEMLAAIPSIIYGMWGLFVFVPLFQEYVQPYLIDTFGQLPFIGM
                     LFSGAPFGIGLFSAGLVLAIMIIPFIASIMRDVFSVVPPLLKESAYGLGSTTWEVIWK
                     VVLPYTKTGVIGAIMLGLGRALGETMAVTFVIGNAFMLPDSVFSPSTSIASAIANEFN
                     EAGGLQKSALMSLGLILFLITSIVLAASRLLIARMGKQEGKRE"
     gene            6310..7158
                     /gene="pstA"
                     /locus_tag="PXD81_001364"
     CDS             6310..7158
                     /gene="pstA"
                     /locus_tag="PXD81_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005650601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HDL5537385.1"
                     /translation="MKNYQNRRFYCRKIKNVLMLSLAYSAVLFGLFWLVWIIVTLLQK
                     GIPALSWELFTRPTPAPNETGGLRNAIVGSILIVSTATLIATPIGILAGTYLSEYGRY
                     TKLAKFTRFLNDVLLSAPSIIIGLFIYAIYVAQVKHYSGWAGSLALAVLVIPIVVRTT
                     DSMLNLVPNNLREAAAALGCPRWRVITMISYRAARSGIITGVLLAVARISGETAPLLF
                     TALSNQFASLDMNAPMANLPVVIYQYAASPFADWNTLAWAGAMLITLFVLSLNIITRL
                     YFSKTK"
     gene            7168..7938
                     /gene="pstB"
                     /locus_tag="PXD81_001365"
     CDS             7168..7938
                     /gene="pstB"
                     /locus_tag="PXD81_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HDL5537386.1"
                     /translation="MLPTDLETKLHINNLNFYYGDFHALKNINMRIAKNKVTAFIGPS
                     GCGKSTLLRTLNRMFELYPNQRATGEINLDGENLLTSDTDISIIRAKVGMVFQKPTPF
                     PMSIYDNVAFGIRLFEKLPKSELNDRVEWALTKAALWNEVKHKLNQNGDSLSGGQQQR
                     LCIARGIAVKPEVLLLDEPCSALDPISTMKIEELINELKHDYTVAIVTHNMQQAARCS
                     DYTAYMYLGELIEFGDTKQIFDKPKFRQTEDYIKGKMG"
     gene            8022..8708
                     /gene="phoB"
                     /locus_tag="PXD81_001366"
     CDS             8022..8708
                     /gene="phoB"
                     /locus_tag="PXD81_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006996031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon transcriptional regulator
                     PhoB"
                     /protein_id="HDL5537387.1"
                     /translation="MNNKILIVEDERAIREMLSLYLSKQYQVIEAEDYQSAINKLGEK
                     PELILLDWMMPGRSGIHLIKYLKKQPETEKIPIIMLTARSSEEDCITGLNAGADDYIT
                     KPFSPQVLLARIEAVWRRSYDPPSNLINIDGLILDQQAQRVLVAEQEVKLSGTEYKLL
                     HFLMTHPEKVYRREQLLDFIWGNDIYVEDRTVDSYIRRLRKSLEPYGFERYIQTVRGS
                     GYRFSNHFQD"
     gene            8711..9991
                     /gene="phoR"
                     /locus_tag="PXD81_001367"
     CDS             8711..9991
                     /gene="phoR"
                     /locus_tag="PXD81_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HDL5537388.1"
                     /translation="MKVKLSLKHFLLELLLAAAVAGVFNLFFPAYFFWFSFCLLVLLI
                     WHHHTESSLLQLLRPNEQSGRSKVTTMEHLSQSAAFYHERLRQDKIKTLRLLSKLNKH
                     IQYLPDAIIICQENGEISWCNNVAQELFEFYWDKKSNKSIFNVIFYPEFKSYFRQQRH
                     HRPLVLLTHNKRYIELNLNRYDSNSRLIVARDVTSFVRLMHSRQTFLANMNHELRTPL
                     TILQGYLEMLENEPHNMALQQKALNAMKEQSQRMTNLLRQLSLLVKIEHSNDSEHNEV
                     QMSALISAIQKNTEILSQNQQILFDIEPNLTVWGDQEQLQSAVSNLIYNAIRHAGEGV
                     TIQICCQRDNQEPNTAIFSVQDDGIGIDAHHITHLTERFYRVDESRSNKTGGSGLGLA
                     IVKHALEQHHSTLEIESEAGKGSRFSFKLPLIKK"
BASE COUNT         2870 a         2234 c         2097 g         2919 t
ORIGIN      
        1 ggattaccta aatattcatt gacaaaacat taataaaacg gtatttctat gcacactttt
       61 caatactgtt gaaaaagagt aatgggcttg ccattacggg agaggtgcaa cactcaggca
      121 acttgatttt aggcaactag ggccgtttga gaattaagtg atgggagtga ttgccgaggt
      181 gtgaattcac tagactggaa ttaacatcgg ctgaataggt tgaatcctat cagctgtcat
      241 ttaataggtt atttcaccat attaaatgga gtgctctgat taattggttc aacaataaaa
      301 ccgtataatc tactcaaatt tcctttccga atatccccta acacaatatt tatctatttt
      361 tgttttaagg aaatttttat tatgtctcat ctttctgtgg ctaaatttgg tggtacaagt
      421 gttgcaaatt ttgatgcaat gacctcttct gccaatattg taattgctga tgcaaatact
      481 cgtgtggtgg tactttctgc ctctgctggc gtaaccaatt acttagtgga acttgctaac
      541 ggctgtgaaa aagaacaccg tgatgaagtc atcgcagcag tccgccaaat tcaatacaat
      601 attattgaaa aattacaaca tcaagatgcc gttaaagcgg aaatcgacga attattagaa
      661 cgtattgaaa cattagcaga atcagccagc cttgctactt cagctgcctt aagtgatgag
      721 ttaatcagcc atggggaaat gatgtccacc aaaatcttca cgcaattatt aattgaacgt
      781 ggacacccgg cagtatgggt agatgtgcgt gacgttgtgg caacaaacag ccattatgga
      841 aaagcagcac caaatgacga gaaaacccag caacaatcag acaatgtgat taagccatta
      901 atcgcatcag gcaaaattgt gattacacag ggctttattg gtcgtgatga tgaaggcaaa
      961 accactactt taggacgtgg cggttcagac tactctgctg cattgttagc agaagtatta
     1021 aatgctaacg atgtactcat ttggacagac gtaccgggga tttataccac cgatcctcgt
     1081 gttgtaccga acgctcaacg cattgacaca atggcattta acgaagcggc agaaatggca
     1141 acatttggag cgaaagtatt acacccagca accttattac cggcagtgcg tagcaatatc
     1201 cctgtatatg ttggctcaag caaagcaccg gaacaaggcg gcacttgggt aacccgtgat
     1261 ccacaaccac gcccgacttt ccgtgcgatt gcattacgcc gcaaccaaat tttactcacg
     1321 ctttccagcc tttcaatgct acacgcacaa ggtttcttag cgaatgtatt tgcgatttta
     1381 gcgaaacaca aaatttcggt tgatgtgatt accacttccg aagtcagcgt tgcattgacc
     1441 ctagataaaa cgggctcagc ctcttcaggt gcagatatgt tatcagcgga attactcgat
     1501 gagcttaacg catattgcaa tgtgcaagta gaaagcgacc tcgctttagt cgctattatc
     1561 ggcaacaact tacacgctca gtccggtgtg gcgaaaaaat tattccacac tcttgagaat
     1621 tacaacattc gcctaatcag ctatggagca agcagcaata atgtttgtac cttagtgaaa
     1681 aatgcagaag cagacgatgt ggttcgttcg cttcacaaag gcttatttga ataagcaata
     1741 actccattgc aagcggttat ttggggcaaa attgttggat ttacccccta accgcttctt
     1801 ctctttactc aacaatctct accgttgaat tggtactaag ccctctcact agctgtgagc
     1861 cttttcttgc tcgttcgcca cggtagttat caacatcgct cggttttaat gtgattttgc
     1921 gtttaccgga cacaaagacc agcgagttgg ttggtttgat gactaataaa cgggctaaat
     1981 attcatcacc ggattttgcc gctgcggcgg aaatattgat gattttattg cctttacctt
     2041 tggaaagttg aggtaattca ctcaccggga aaaccaacat tctgccaaca ttactcatcg
     2101 ctacaagcga taaattttca tcattttcca ataattgcgg cggcagtact tttgcatttt
     2161 cggttaaaga aataaccgct ttgccggctt tattacgaga aactaaatct gcaaaggtgc
     2221 aaataaagcc gtaacctgaa tcggatgcca ttagcacttt agtttctgga cttgccatta
     2281 gcacttgctg cactactgca ccttccggta gggtgatttt gcctgtaagc ggttcgcctt
     2341 gcgagcgtgc cgaaggcagt gaagttggat cgagagcata agctcgccct gtactgtcta
     2401 aaaataccac aggctgatta cttctacccc ttgcgtgggc aagataacca tctccggctc
     2461 gatggcttag ggcttgaaca tcaatatcgt gtcctttcgc acaacgtacc cagccttttt
     2521 cagacaaaat cacagtcaca tcttcggttg gagtcaagtc actttccgca atcgctttag
     2581 actccgctcg ttcaaccaat ggtgagcggc gtgggctggc aaatgctttg gcatcggctt
     2641 gaatctcttt cttgatcagg ctgttcaaac ggcgttccga gcctaaaatc agttgcaatt
     2701 catcacgttc tttttccagt tcggattttt ccgcctgtaa ttgatgctct tccaatttgg
     2761 ctaagtggcg taaacgtaag ttcaggatcg cctctgcttg cacatcggtc agattaaaac
     2821 gagccattaa ctcggctttc ggctcatctt cattgcgaat aatctcaatc acttcatcaa
     2881 tattgagaaa tgcaatcatc aaaccgtcta aaatatgcaa gcggtttaaa attttatcta
     2941 atcggtaatt caggcggcga gtgacagtgg tacgtctaaa actcagccac tcattgagaa
     3001 tagtcagcaa attcttcact gccggcttgc cgtctaagcc aatcatattc atattgactc
     3061 ggtagctttt ctccaaatcg gtcgtcgcaa acaggtgatc cattaacgca tcaaaatcaa
     3121 tgcgattaga gcgtggcaca atcacaatgc gaatcgggtt ctcgtggtca gattcatcac
     3181 gaatatcgtc caccatcggt agttttttat tccgcatttg ggttgctact tgctcaataa
     3241 tttttgaagg agaggcttgg tgcggcaagg ctgaaatcac aatttcaccc tcttcttttt
     3301 tccacaccgc acgcatttta atagaaccac gcccctgctc atacattctg gcaatttccg
     3361 cttttggtgt gatgatttca gcctctgtcg ggtaatccgg cccttgtacc acgcttagta
     3421 catcatcaag cgttgctttc gggttatcca gcaacatcac actggcatca gccaattcat
     3481 taatattgtg tggtggaata tcggttgcca ttcccaccgc aatccccatc gtgccattca
     3541 acaaaatatg cggtaaacga gccggcagat atttcggttc ttccaacgag ccatcaaaat
     3601 ttggctgata atccaccgtt ccttgcccta actcgcccaa taaaatttct gcaattttag
     3661 acagtttcga ttcggtataa cgcattgcag cgaaggattt cggatcgtcc ggagcccccc
     3721 agttgccttg tccgtccact aacggatagc ggtaagagaa cggttgagcc attagcacca
     3781 ttgcttcata gcaggcacta tcaccgtgcg gatggaattt acctaataca tcccccacgg
     3841 tacgggctga ttttttatac tttgccgagg cgtttagccc aagttccgac atcgcataaa
     3901 taatacggcg ttgcaccggt ttcaagccat cgccaataaa cggcaatgcc cggtccataa
     3961 ttacatacat cgaataattg aggtaagcat cttcggtaaa acgcttaatc ggcatctgtt
     4021 caatgccttc ataattgatt tctgtggtca ttttttatcg atatttatat taaaaaaacg
     4081 agggctattt tctacgattt agcctgttta gtctagcctg aaacacaagc ggtcgttttt
     4141 tctgattttt ttaccattgt catcattttg tcatattctt tttctacact aagcacgtga
     4201 aattaactca cgctaacttt aaggagaaca atatgattgc caaacgattt aaactactta
     4261 ccgttttagg catttctttt tcactttctg tcgcagcaaa tgctcaaacc atcacaggag
     4321 ccggtgcttc tttcccttat cctatctacg ccaaatgggc aggtttatat gaaaaagaga
     4381 ccggtaataa aatcaattac caatctatcg gttccggtgg cggtcagcaa caaattattg
     4441 ccaaaacaat tgatttcggt gcttctgatg acccgatgaa aggcgaaaca ttagcagaac
     4501 acaaactttt gcaatttccc gccatcattg ggggaactgt accggttgtc aatattcctg
     4561 aacttaaatc gggcgaactg aaactgtccg gtaaagttct tgccgatata ttcctaggca
     4621 aaattacaca atggaacgat ccggcaattg tcgcacttaa tcaagatatc gccctgccgg
     4681 caaaaacaat tttagtcgta cgccgctcag acggttcagg cacaactttc ggttggacaa
     4741 actatctttc taaagtctct gccgaatgga aggagaaagt cggcgaagga aaatcggtga
     4801 aatggccggt cggacaaggc ggaaaaggaa atgaaggtgt cgcagcttat gtgaaacagc
     4861 tcaagtattc tattggttac gtagaatatg cctatgctaa acaaaatgct ctggcttggg
     4921 tttctatgca aaatgcagat ggtaaatttg ttcagccttc ccgtgaaacg tttgctgctg
     4981 ccgctgctaa cgccgattgg agccaaacag ccggtatggg cgttatttta accaacgaag
     5041 cgggagaaaa agcctggccg attaccgccg ccagttttat cttgattcat caacatagcg
     5101 acaaaccaga gcaaactaaa ggggtattta aattcttcga ctgggcattt aagcacggta
     5161 aagatgcggc tgccgaactg gattatgtgc ctctaccgga taatgtagta gagaaaatcc
     5221 aaactcaatg gaaaacggaa gttaaagatg gacaaggaaa ttcgctctac taatgcaaaa
     5281 cgaccgcttg tatggagctt aactaacaac aagcggtcgt tttttcacaa cattttgcaa
     5341 aagagttgtc actatgcata aacataaaat tgataacaaa tgcttaaacc atccttgggt
     5401 agaggggtta tttcatcaca caacccgttt ctttgctttg ctggtttttg ccttgttggt
     5461 ggtggtgctg gtttcattat tgcttggcag ttgggaagca ttaagccgat tcggggtatc
     5521 attcctatgg gaaaacgagt ggaaccctgt gaatgaccga tacggtgcat tagtgccgat
     5581 tatcggcaca ttgatttctg ccggcattgc attagtcatt gccgttccgg tctcgttcgg
     5641 cattgcgact ttcttaaccg aacttgcacc gcaatggcta aaacgccctg tcggcacagc
     5701 ggtagaaatg ctagcggcta ttccctctat tatttacggt atgtgggggc tgtttgtatt
     5761 tgtaccctta tttcaagaat atgtgcaacc gtatttgata gatacatttg gtcagcttcc
     5821 ctttatcggt atgcttttta gcggagctcc tttcgggatc gggctattca gtgccggctt
     5881 ggtattggcg attatgatta ttccctttat tgcctcaatt atgcgtgatg ttttcagtgt
     5941 tgtaccgcct ttactgaaag agtctgctta cggtttaggc tcaaccactt gggaagttat
     6001 ttggaaagtc gtgctacctt acaccaaaac cggagtaatc ggtgcaatta tgctcgggtt
     6061 aggtcgagca ttaggcgaaa caatggcagt tacctttgtg atcggtaatg cctttatgct
     6121 acctgattcg gtattttccc cttcaacctc catcgcttct gctatcgcca atgaatttaa
     6181 tgaagccggc ggattacaaa aatcagcgtt gatgagccta gggctaattc tgttccttat
     6241 tacttcgatt gttttagccg cttcacgttt attaatcgca cgaatgggta aacaggaagg
     6301 aaaacgagaa tgaaaaatta ccaaaaccga cgcttttatt gccgaaaaat aaaaaatgtc
     6361 ttgatgttaa gtcttgccta ttccgctgta ttatttggct tattttggct ggtctggatt
     6421 attgttactt tactccaaaa aggtatccct gcactgtctt gggaactgtt tacccgcccg
     6481 actcctgcac caaatgaaac aggaggatta cgtaatgcaa ttgtcggctc tatactgatt
     6541 gtcagtaccg caacactgat tgcaacacca atcggtattt tagccggcac ttacttgagc
     6601 gaatacgggc gttataccaa attggcaaaa ttcacccgtt ttttgaatga tgtgttgctc
     6661 tctgctcctt ctattattat cggcttattt atttatgcga tttatgtcgc acaggttaaa
     6721 cactattcag gctgggcagg ctcattagca ttggcggtat tagtgattcc gattgttgtt
     6781 cgaactaccg acagtatgct caatcttgtg cctaataatc tgcgtgaagc agctgcggct
     6841 ctcggctgcc cacgatggag ggtaattaca atgatctctt atcgtgccgc ccgctccggc
     6901 attattaccg gcgtgttgct cgctgttgcc cgtatttccg gagaaaccgc accgctattg
     6961 tttaccgcat tatccaacca gtttgcctct ttagatatga atgcaccaat ggcaaacctc
     7021 cctgtggtga tttatcaata cgcagccagc ccgttcgctg actggaatac attggcttgg
     7081 gcaggtgcaa tgcttattac tctgtttgtg ctttctctta atatcatcac tcgtctgtat
     7141 ttcagcaaaa caaaataagg actctatatg ttaccaacgg atttagagac aaaattacat
     7201 attaacaacc tcaattttta ttatggcgat ttccacgctc tgaaaaatat caatatgcgt
     7261 attgccaaaa ataaagtgac cgcatttatc ggtccttccg gttgtggaaa atcaacatta
     7321 ctgcgaaccc taaaccgaat gtttgagctc tacccgaatc aacgtgcaac gggggaaatc
     7381 aatcttgatg gagagaatct actcacctcc gatacggata tttcgattat tcgagccaaa
     7441 gtcggtatgg ttttccaaaa accgacccca ttcccgatgt ccatttacga taatgttgct
     7501 tttggtattc gtctgtttga aaaattgccg aaatccgagc taaacgatcg tgtagagtgg
     7561 gcattaacca aagcggcttt atggaatgag gtaaagcata aattaaacca aaatggtgac
     7621 agtctctccg gcgggcaaca gcaacggctc tgtattgcac gaggtattgc agtgaaaccc
     7681 gaggtgttac tgctggatga gccctgctcc gcacttgacc cgatttcgac tatgaaaatc
     7741 gaagaactta tcaacgaact caagcacgac tacacggtag cgatagttac ccacaatatg
     7801 cagcaagcag cccgctgttc tgactatacc gcttatatgt atctaggcga gctgattgaa
     7861 ttcggtgata ccaaacaaat tttcgacaaa ccaaaattcc ggcagacgga agattacatt
     7921 aaaggtaaaa tggggtaatc tgcaagcggt cggttttagt aaaaaatttg caaatttctg
     7981 cgataaatcg accgcttgtc acaccgcatc ggagaggaag aatgaataac aaaattctga
     8041 tcgttgaaga cgaacgtgct attcgtgaaa tgctaagtct ctatctcagc aaacaatatc
     8101 aggtaattga agcggaagac taccaaagtg ctatcaataa actgggcgaa aaacccgagc
     8161 ttattttact ggattggatg atgccggggc gttcgggtat ccatttaatt aaatacctaa
     8221 aaaaacagcc tgaaaccgaa aaaattccaa tcattatgct aacagcccgc agtagcgaag
     8281 aagactgtat taccggtctg aatgccggtg cagacgacta cattaccaaa ccgttttcac
     8341 cacaggtgtt gcttgcccgg attgaagcag tatggcggcg aagctatgat ccgccaagca
     8401 atctcatcaa tatagacgga ttgattcttg atcaacaggc acaacgggtt ttggttgccg
     8461 aacaggaagt gaaattaagc ggcacggaat ataaactgct tcacttttta atgactcacc
     8521 ctgaaaaagt gtatcgtcgt gaacagttgc ttgattttat ttggggtaat gatatttatg
     8581 ttgaggatcg cacggtagac tcttatattc ggcgattgcg taagagtctt gaaccctatg
     8641 gttttgagcg ttatattcaa accgtacgtg gctcgggata ccgtttttcc aaccattttc
     8701 aggactaata atgaaagtta agctctcact taaacacttt ttattggaac ttttgctggc
     8761 agcagccgtt gccggcgtat tcaatctgtt tttccctgct tattttttct ggtttagttt
     8821 ttgtttactg gttctactta tttggcatca ccacaccgaa tcttctctcc ttcagttact
     8881 cagacctaat gagcaaagcg gcaggtcaaa agtaacgaca atggagcatt tatctcaaag
     8941 tgccgcattc tatcacgaac gtttacgcca agataaaatc aaaaccttac gtctgttatc
     9001 aaaattaaat aagcatattc aatacttacc cgatgcgatc attatttgcc aagaaaacgg
     9061 ggagatttct tggtgcaata atgttgcaca agagctattt gagttttatt gggacaaaaa
     9121 atcaaataaa agcattttta atgtgatttt ttatcctgaa tttaaaagct atttccgcca
     9181 acaacgacac catcgcccat tggtactgct gactcataat aaacgctata ttgaattgaa
     9241 tctcaatcga tatgacagta actccagact tattgtcgca cgagatgtaa cctcttttgt
     9301 ccgccttatg cactcacgac aaaccttttt ggcaaatatg aaccacgaac tgcgaacacc
     9361 tcttaccata ttacaaggtt atttggaaat gctggaaaac gagccgcata atatggcatt
     9421 acagcaaaaa gcgctcaatg cgatgaaaga gcaaagtcaa cgtatgacta acctgcttcg
     9481 ccaactctca cttttggtta aaatcgagca ttccaatgat tccgaacaca atgaggtcca
     9541 gatgtcggcg ttaatctccg ccatacaaaa aaataccgag attttaagcc aaaatcagca
     9601 aatactgttt gatattgagc ctaatttaac ggtatgggga gatcaagagc aactccaaag
     9661 cgctgtatca aatttaattt acaatgcaat acggcacgcc ggtgaaggag ttaccattca
     9721 aatatgttgt caacgtgata atcaagagcc gaatactgcg attttcagcg tgcaagatga
     9781 cggtattggt atcgatgccc atcatattac ccatctgaca gaacgtttct atcgtgtgga
     9841 cgaatctcgt agcaacaaaa ccggcggtag cggtttaggt ctggctattg tcaaacacgc
     9901 tcttgaacag caccattcca cactggaaat tgaaagtgaa gccggtaaag gaagcagatt
     9961 tagttttaaa ctacctctaa taaaaaaata gcgatgcaat caaactattt tcacaaggct
    10021 tttcacaaac taatccccat tttacactga agttttgaat ttctttataa ctgaggtgca
    10081 agatcaaaat aataggcgat tttttataaa aatcacgcta
//
LOCUS       DANLYI010000077         9889 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.077, whole genome shotgun sequence.
ACCESSION   DANLYI010000077 DANLYI010000000
VERSION     DANLYI010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 9889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9889
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.077"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            8..745
                     /gene="tsaA"
                     /locus_tag="PXD81_001368"
     CDS             8..745
                     /gene="tsaA"
                     /locus_tag="PXD81_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HDL5537389.1"
                     /translation="MNSTSNQLTPIQLNPIGFVESPYDEKFSVPRQPNLVAEGKGILR
                     LIPPYNTPDAVRGLEKFSHIWLIFQFHHIPEREWHATVRPPRLGGNERVGVFASRATH
                     RPNPLGLSKVKLEAVVAGGSEVYLKLGSVDLVNGTPIFDIKPYIAFADSEPQAVSGFA
                     QQKPQARLAVRFSEEAKQAVEFCRNFAKFGIEQPLSFLADVIAQDPRPAYQQGKPSER
                     VYGMNLAGYNIRWQIENDTQAIVLSIE"
     gene            803..1522
                     /locus_tag="PXD81_001369"
     CDS             803..1522
                     /locus_tag="PXD81_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253715.1"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HDL5537390.1"
                     /translation="MKLKNFLTILPLAVATAVTSAETHTVEHKDKPASFNFSTEVSRT
                     VEKDVMQASVYSQKSGNSLPELKKFVSENLNRVLAEAKKHPSIEVQAEGVTNHVNYNN
                     KGNIDGWDTSGSIQLKSKDFDAMAAVLENLGKDVAIRYIDFSVSPEKMASLEDEMTLE
                     IISKFQHKADVIQKALGAKTYKLVNINIQTPNDGGRPYYEHRAMYAVSSMAQSKSMDE
                     IPLEAGKTTISATASGSVEFE"
     gene            1710..2396
                     /gene="aqpZ"
                     /locus_tag="PXD81_001370"
     CDS             1710..2396
                     /gene="aqpZ"
                     /locus_tag="PXD81_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005617943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HDL5537391.1"
                     /translation="MKKYFAEFFGTFWLVFGGCGSAVLAAGIPELGIGYAGVSLAFGL
                     TVLTMAYAVGHISGGHFNPAVSIGLLVGGRFNAKDLVPYIIAQVIGAIAAGAVLYTIA
                     SGVPTFDATAGFAGNGYGEHSPHGYSLTAALLIEVVLTAFFLIIIMGATDKRAPAGFA
                     PIAIGLALTLIHLISIPVTNTSVNPARSTGVALFQGSWAIEQLWLFWVAPIVGAIIGA
                     VVYRFIAEEK"
     gene            2468..2608
                     /locus_tag="PXD81_001371"
     CDS             2468..2608
                     /locus_tag="PXD81_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5537392.1"
                     /translation="MKKLIKVCGLISATIFLSGCLVTSVVGGVVGTAVDVVDTVTPDI
                     ID"
     gene            2711..3304
                     /gene="slyD"
                     /locus_tag="PXD81_001372"
     CDS             2711..3304
                     /gene="slyD"
                     /locus_tag="PXD81_001372"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015674369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDL5537393.1"
                     /translation="MNITKDLVPSIAYQVRTQEGVLVDEAPVNQPLEYLHGHNNLVEG
                     LEKALEGKAVGDVFEVRVKPEEGYGEYNDNMVQRVPKDVFMGVDELEVGMRFIADTDL
                     GPLPVVITEVDGDEVVVDGNHMLAGQELLFSVEVVGIREATAEEIAHGHVHGGHEHGC
                     GCGGHDHHHDHDGCCGGHDHDHHHHHGHGGCGCGGHH"
     gene            3565..5097
                     /gene="ilvA"
                     /locus_tag="PXD81_001373"
     CDS             3565..5097
                     /gene="ilvA"
                     /locus_tag="PXD81_001373"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HDL5537394.1"
                     /translation="MMNETQHSQSAIDYLRSILSSNIYDLAQVTPLQKMEKLSERLGN
                     SIFIKREDRQPVHSFKLRGAFAMISNLSKAQKEAGVIAASAGNHAQGVALSAKHLGLR
                     ALIVMPQNTPAIKVDAVRGYGGEVLLYGANFDEAKAKAIELSTELGMTFIHPFDSPAV
                     IAGQGTIGLELVQQKPDLDYVFIPVGGGGLIAGIAVLIKQVMPNIKVIGVESKDSACL
                     YHALKQGQPTDLERVGLFADGIAVKRIGDETFRLCQQYVDDVVLVDNDEICASMKLIF
                     ENVRAISEPSGATSLAGLKKYVKEHQLQDKNLACILSGANVNFHTLRFVSERCEIGEK
                     REALLAVTIAEQKGSFLKFCEILGNRAVTEFNYRHSDDKQACIFVGVRISGSAEKVEI
                     IKDLQQNGYDVADLSDDDIAKTHIRYMVGGRASSIQYEQLYSFEFPEQKGALLKFLQM
                     LTNWDISLFHYRAHGADYGDILAAFALNRGDEVELNRHLEQLGYRFQNVSESPAYQYF
                     LK"
     gene            5123..6457
                     /gene="glmM"
                     /locus_tag="PXD81_001374"
     CDS             5123..6457
                     /gene="glmM"
                     /locus_tag="PXD81_001374"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015939592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HDL5537395.1"
                     /translation="MARKYFGTDGVRGEVGKFPITPEFALKLGWAAGKVLATQGTKKV
                     LIGKDTRISGYMLESALEAGLTAAGLSAVFVGLMPTPAIAYLTRTFRAEAGIVISASH
                     NPYYDNGIKFFSSVGEKLPDEIEEAIEAMLDKPMDCVDSANLGRASRINDAAGRYIEF
                     CKGTFPSELSLEGYKIVVDCANGATYHIAPNVMRELGAEVLEIGTKPDGLNINEKCGA
                     TDIKALQKVVVEAGADVGLAYDGDGDRLIMVDHLGNKVDGDQILFILAREALRSGKLQ
                     GGVVGTLMSNMSLELALKELAIPFSRANVGDRYVLEQLKEKGWKLGGENSGHIIVLDK
                     NTTGDGIIASLQVLAAMASHKLSLNDLAKAVPLFPQVLLNVRFEKGAANPLESNEVKA
                     VAADVEKRLAGKGRILLRKSGTEPLIRVMVECEDAVLAQSCAEEIVEAVKRN"
     gene            6602..7261
                     /gene="rpiA"
                     /locus_tag="PXD81_001375"
     CDS             6602..7261
                     /gene="rpiA"
                     /locus_tag="PXD81_001375"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HDL5537396.1"
                     /translation="MDQQEMKKIAAQAALKFVKPDTIVGVGSGSTVNCFIDALASMKD
                     EIKGAVAASKASEQRLRDIGIEVFSANEVTELDVYIDGADEITPQGAMIKGGGAALTR
                     EKIVSSLAKKFICIVDKSKQVDVLGSTFPLPVEVIPMARSYVARQLVALGGSPEYREG
                     VVTDNGNVILDVHNFKIMEPLKMEHTINNIAGVVTNGIFAQRFANVTIVGTPEGAVIL
                     E"
     gene            7298..8527
                     /gene="serA"
                     /locus_tag="PXD81_001376"
     CDS             7298..8527
                     /gene="serA"
                     /locus_tag="PXD81_001376"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HDL5537397.1"
                     /translation="MAKISLDKSKIKFLLLEGVHQNAIDVLLAAGYTNIEVHKKALDG
                     QELIDAIKDAHFVGIRSRTFLTEEVLAQAPKLIAIGCFCIGTNQVDLNAAKRRGIPVF
                     NAPFSNTRSVAELVLAEMILLMRKVPTANAEVHRGVWNKSAAGANEVRGKKLGIVGYG
                     HIGSQLSILAEAIGMQVYFYDVENKLPLGNAQQVPTLEHLLSTCDMISLHVPEIDSTK
                     NLMSAERIAQLKEGSVLINAARGTVVDLDALAARIEDGSLRGAAIDVFPEEPASINDP
                     FVSPLCKFDNVILTPHIGGSTSEAQANIGTEVANKFVKYSDNGSTVTAVNFPEVSLPL
                     HTNTKRLLHIHENRPGMLNQINQIFVESNVNIAAQYLQTDAAIGYVVIDVESDNVDEA
                     LQRLKAIEGTIRARVLY"
     gene            complement(8605..9123)
                     /locus_tag="PXD81_001377"
     CDS             complement(8605..9123)
                     /locus_tag="PXD81_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018650931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HDL5537398.1"
                     /translation="MKKLTIVALTCLFSTHLLAAPVGNTFTGIGVGVDVTTTKYKVEG
                     VKGKDSTGPALVVDYGIEQGNNFVGVVQGKAKVGSTKVFSDVKQKNKYTIAYQQGYRV
                     GSDLLPYVKVDASSSKIGNETYRGFGFGAGAKYAVSSDVELGAEYTRDNLKRSGTKLK
                     GNAFTANATYRF"
     gene            complement(9143..9865)
                     /gene="arcA"
                     /locus_tag="PXD81_001378"
     CDS             complement(9143..9865)
                     /gene="arcA"
                     /locus_tag="PXD81_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HDL5537399.1"
                     /translation="MKIPQILIVEDELVTRNMLKSIFEGEGYQVFQASNGEEMHSVLS
                     TNEINLVLMDINLPGKNGLILAREIRENMKLPLMFLTGRDNEIDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLQRTMIDKENDVVKEQSEIESYRFNGWILDINSHSLISPEGEV
                     NKLPRSEFRALLHFCQHPGKIQTREDLLKKMTGRELKPHDRTVDVTIRRIRKHFEDHP
                     NTPEIIVTIHGEGYRFCGNIEP"
BASE COUNT         2855 a         1876 c         2292 g         2866 t
ORIGIN      
        1 aagcgtaatg aactctacct caaatcaact aaccccgatt caattaaacc ctatcggttt
       61 tgtggaaagc ccctatgatg aaaaattttc cgtgcctcgc cagccgaatt tggtagcgga
      121 ggggaaaggc attctacgtt taattccgcc ttacaatacc cccgatgcag tcagaggctt
      181 ggagaaattt agtcatattt ggttgatttt ccaattccat cacattcccg aaagagagtg
      241 gcacgccacc gtcagaccgc ctcgcttggg cgggaacgaa cgggtcgggg tctttgccag
      301 ccgtgccacc catcgtccga atccgcttgg cttatcgaaa gtgaagttag aagcggtagt
      361 ggcggggggg agtgaagttt atttgaaatt aggcagcgtg gatttagtta acggcacacc
      421 aatttttgac atcaagcctt acattgcatt tgcagatagc gagccgcaag cggtttccgg
      481 ttttgctcag caaaaaccac aagcaagatt agcggtgcgt tttagcgagg aggcaaaaca
      541 agctgtcgaa ttttgccgaa attttgcaaa atttggcatc gaacagccgc ttagttttct
      601 tgccgatgtc atcgcccaag accctcgccc ggcgtatcaa caagggaaac ctagtgagcg
      661 agtttatggt atgaaccttg ccggctacaa tattcgctgg caaattgaaa atgatacaca
      721 ggcgattgtg ctttcgattg aatgaacttt cctacgcaag tgagatctaa gaaatgattt
      781 ttaacccttt taaggactga ttatgaaatt gaaaaatttt cttaccattc taccgcttgc
      841 agtcgcaaca gcggtaactt cagcggaaac tcataccgtg gaacataaag ataaaccggc
      901 ttcttttaat ttctcaaccg aagtctctcg cacggtggaa aaggacgtga tgcaggcaag
      961 tgtgtatagc caaaaatcag gtaactcctt gccggagctg aaaaaatttg tatctgaaaa
     1021 cctgaatcgt gtattggctg aggcaaaaaa acatccatcg attgaagtac aagctgaagg
     1081 tgtgactaat cacgttaatt ataacaacaa agggaatatt gatggttggg atacctctgg
     1141 tagcattcag ttgaaaagca aagattttga tgcaatggca gcggtgttgg aaaatttagg
     1201 taaagatgtg gcgattcgtt acattgattt tagtgtatcg ccagagaaaa tggcgtccct
     1261 tgaagatgaa atgacgctgg aaattatcag caaatttcag cataaagccg atgtgattca
     1321 aaaagcctta ggggcgaaaa catataagct agttaatatc aatattcaaa ctccaaatga
     1381 tggtggtaga ccatattatg aacacagagc aatgtatgca gtttcatcaa tggcacaaag
     1441 caaatctatg gatgaaatcc cgttagaagc aggtaaaacc acaatttcag ctaccgcttc
     1501 aggaagtgtg gaatttgaat agaaatgtat agatttgtat agagttgtat ttttacttta
     1561 attggaggaa aaaatcggtc tatactatca attaagaaat atgcgtcttt acttttttta
     1621 gtatactcat attttattgg ctatgtttta tagcattcgg aaagttaata cacttatgtg
     1681 tgattcaccc tttagggtaa ggataaaaaa tgaaaaaata ttttgctgag tttttcggga
     1741 cattttggtt agtgtttggt ggttgcggca gtgcagtttt agcagcaggc attcctgaat
     1801 taggaatcgg ttatgcaggc gtttcgttag ccttcggttt aaccgtatta acaatggcgt
     1861 atgcagtcgg tcatatttca ggtggtcact tcaacccggc ggtttcaatc ggtttattag
     1921 tcggcggtcg ttttaacgct aaagatttag tgccttatat cattgcccaa gtaatcggtg
     1981 cgattgctgc cggtgcagtg ttatacacca ttgcttcagg tgttccgact tttgatgcta
     2041 cagcaggctt tgccggcaac ggttatggtg agcattcgcc acacggctat agcttaacag
     2101 cagcattatt aattgaagtg gtattaacgg cattcttctt aatcatcatt atgggtgcaa
     2161 ccgataaacg tgctccagca ggtttcgctc cgattgctat tggtttggct ttaaccttaa
     2221 tccacttaat cagcattccg gtaaccaata cctcagtaaa cccggcacgt tcaaccggtg
     2281 tagcgttatt ccaaggcagc tgggcgattg agcaattatg gttgttctgg gttgctccga
     2341 ttgtcggtgc aattatcggt gcggtagttt atcgctttat cgctgaagaa aaataattta
     2401 agcgaataaa tatgatgcag ctctagtctt gattagggct gttttttgtt tttgaaagga
     2461 ttttaaaatg aaaaaattaa tcaaagtatg tgggctgatt tcagcaacta ttttcctaag
     2521 tggttgtttg gtaacttcgg tggttggtgg agtggtggga accgcagttg atgtggtaga
     2581 caccgtaacc cctgatatta tcgactaatt tactgacaag cggttaaaat ttccagatag
     2641 attgcaaata taaaataaag cgttacaatg gcttgctatt ttataaaacc gatgaattaa
     2701 aggattcaat atgaacatta caaaagattt agtgccaagc attgcatatc aagtgcgtac
     2761 tcaagaaggc gtattagtag atgaagcacc ggtaaaccaa ccgttagaat atttacacgg
     2821 tcacaacaac ttggtagaag gcttagaaaa agcccttgaa ggcaaagctg taggcgatgt
     2881 gtttgaagtg cgtgtgaaac cggaagaagg ttacggcgag tacaacgata atatggttca
     2941 acgtgtaccg aaagatgtat ttatgggcgt cgatgaatta gaagtgggta tgcgttttat
     3001 tgcggacacg gatttaggtc cgctaccggt ggtaattacc gaagtagatg gcgatgaagt
     3061 tgtggtggac ggtaaccata tgttagccgg tcaggaatta cttttctctg tggaagtggt
     3121 tggcattcgt gaagcgacag cggaagaaat cgcacacgga cacgttcacg gcggacacga
     3181 acacggttgc ggttgtggtg ggcacgatca tcatcacgat cacgatggtt gctgcggcgg
     3241 tcacgaccac gatcatcacc atcatcacgg acacggtggc tgtggttgcg gtggtcatca
     3301 ctaactttgt gatttcccct ctccccgctt gcgggagagg gtggagcaaa tcgaagattt
     3361 gcggagggag agggggatat ccaaggtttc ccctctctct gcttaaaatt tctgaacgaa
     3421 attttaagct gtctctctcc cgcaaacggg cgagaggaaa tattccctgt taaagccatt
     3481 gctaaataac caaacaagcg gtcaattttc cttggaaatt taccaatgtc tttatctcaa
     3541 tctaacaata aataaccttt caaaatgatg aacgaaacac aacattccca atccgctatc
     3601 gactatttac gctcgatttt aagctcaaat atttatgact tagcgcaagt gaccccttta
     3661 cagaaaatgg aaaaactgtc tgagcggtta ggcaactcta tttttatcaa acgtgaagac
     3721 cgacagccgg ttcacagctt taaattgcga ggtgcttttg cgatgatctc aaatttatcc
     3781 aaagcacaaa aagaggctgg agtcattgcg gcttcagcag gcaaccacgc acaaggggtg
     3841 gcactttctg ccaaacattt aggtttaaga gccttgattg taatgccgca aaacacaccg
     3901 gcaattaagg ttgatgcggt acgtggctat ggtggtgaag tgttgcttta tggggcgaat
     3961 tttgatgaag ctaaggcgaa agcgattgag ctttccaccg aattgggtat gaccttcatt
     4021 cacccgtttg acagcccggc agtgattgca gggcaaggca caatcggctt agaactggtg
     4081 caacaaaagc cggatttaga ttatgtattt attccagtgg gcggtggcgg cttaattgcc
     4141 ggcattgcgg ttttaattaa gcaggtgatg ccgaatatta aagtgattgg ggtcgaatct
     4201 aaagattctg cttgcttata tcacgcctta aagcaagggc aaccgaccga tttagaacgg
     4261 gttgggcttt ttgctgacgg tattgcggta aaacgcattg gcgatgaaac tttccgcctg
     4321 tgtcagcaat atgttgatga tgtggtgttg gtggataatg atgaaatctg tgcctcaatg
     4381 aagttgattt ttgaaaatgt gcgtgcgatt tccgagccgt ccggggcaac ttcccttgcc
     4441 ggtttgaaaa aatatgtgaa agagcatcaa ttacaagata aaaacttagc ttgcattctt
     4501 tccggggcga atgtgaattt ccacacctta cgttttgtgt cagagcgttg tgaaatcggg
     4561 gagaaacgtg aagctttatt ggcagtcacc attgctgaac agaaaggtag tttcttaaaa
     4621 ttctgcgaga ttttaggcaa ccgtgcggta acggaattta actatcgcca ttccgatgat
     4681 aaacaagcct gtatttttgt gggggtgcga atttcaggct cggcagaaaa agtagagatt
     4741 attaaagatc tgcaacaaaa cggctatgat gtggcagatt tatcggacga tgatattgcc
     4801 aaaacccata ttcgttatat ggtaggcggt agagcaagct ccattcaata tgagcaactc
     4861 tatagctttg aatttccaga gcagaaaggg gcattgctaa aattcttaca aatgctgact
     4921 aactgggata tctcgttatt ccattaccga gctcacggag ccgattatgg cgatattctt
     4981 gccgcatttg cattaaacag aggcgatgaa gtagaattaa accgacattt agaacagcta
     5041 ggttaccgct tccaaaatgt gagcgaaagt ccggcatatc aatatttttt aaagtaaatt
     5101 tttatcaatc ttaggaatta gaatggctcg taaatatttc ggcacagacg gtgtgcgtgg
     5161 tgaagtaggt aaatttccaa ttacacctga atttgcatta aaactcggtt gggcagcagg
     5221 taaagtgttg gcaacccaag gcacgaaaaa agtattaatc ggtaaagata cccgtatttc
     5281 cggttatatg ttggaatcgg ctctggaagc cggtttaacc gcagccggtt tatcagcagt
     5341 attcgttggt ctgatgccga ctccggcaat tgcttattta acccgtactt tccgtgcgga
     5401 agccggcatt gtgatttctg cctctcacaa tccttattac gataacggca ttaaattctt
     5461 ctcgtcggtt ggtgaaaaac tacctgatga aattgaagaa gcgattgaag caatgcttga
     5521 taagcctatg gattgcgtag attcagcaaa tttaggtcgt gccagtcgta ttaatgatgc
     5581 cgcaggacgt tatatcgagt tttgtaaagg cactttcccg tctgaactaa gccttgaagg
     5641 ctataaaatt gtggtggatt gtgccaacgg tgcaacctac catatcgcgc cgaatgtaat
     5701 gcgtgagcta ggggcagaag tgcttgaaat cggtactaag ccggacggtt taaacatcaa
     5761 cgaaaaatgt ggtgcaacgg atattaaagc acttcaaaaa gtggtggtag aagcaggtgc
     5821 ggatgttggt ctagcttacg atggtgatgg cgaccgtttg attatggtcg atcacttagg
     5881 caataaagtt gatggcgacc aaattctatt tattttagcc cgagaagctc tacgttcagg
     5941 caaattacaa ggcggtgtag tcggtacatt aatgagtaat atgagcttgg aattagcttt
     6001 aaaagagcta gccattccat tctctcgtgc taacgtgggt gaccgctatg tactagagca
     6061 gcttaaagaa aaaggctgga aactgggtgg cgaaaactcc gggcatatta tcgtgttaga
     6121 taaaaacacc accggtgatg gtattattgc ctcactgcaa gtattagcag caatggcaag
     6181 ccacaaatta agcctgaatg atttggcaaa agcagtgcct ctgttcccgc aagttctgct
     6241 aaatgttcgc tttgagaaag gggcggcaaa tccgcttgaa agtaacgaag tgaaagcggt
     6301 agcggcagat gtggaaaaac gcttggcagg caaagggcgt attttattgc gtaaatcagg
     6361 cactgagcca ttaattcgag tgatggtgga gtgcgaagat gctgtacttg ctcaaagctg
     6421 tgcggaagag attgttgaag cagtaaaacg taactaatga caagcggtcg tttttggtaa
     6481 aaaatttgcc aaatatgacc gcttgttaca tatatgtgta gaatagatgt agtagggcga
     6541 aaattttttt cgccttgccg aagtgggcaa atgatgattt gccctacaag tggaggtaaa
     6601 aatggatcaa caagaaatga aaaaaattgc ggcacaagct gcattaaaat ttgtaaaacc
     6661 ggacactatt gttggggtgg gaagtggttc aaccgtaaac tgttttattg atgcccttgc
     6721 ttcaatgaaa gatgagatta aaggagcggt tgctgcctct aaagcctcgg aacagcgttt
     6781 gcgtgatatc ggcattgaag tattcagtgc caatgaagtg acggaattag atgtgtatat
     6841 tgacggagct gatgaaatca cgccacaagg ggcgatgatt aaaggtggcg gggcagcact
     6901 tacccgtgag aaaatcgtga gttcattagc gaaaaaattt atctgcattg tggataaatc
     6961 caaacaagtt gatgtgctgg gttcaacttt ccctctgccg gtggaggtga tcccaatggc
     7021 tcgttcttat gtggctcgtc agctcgtagc cttaggtggt tcgccggaat accgtgaggg
     7081 cgtggtcact gataacggca atgtgatttt agatgtacat aactttaaaa ttatggagcc
     7141 gttgaaaatg gaacatacca ttaataatat tgcaggcgtg gtgactaacg gtatttttgc
     7201 acaacgtttt gccaatgtaa ccattgtcgg cacacctgaa ggtgcagtta ttttagaata
     7261 atttattatt gcaaatacaa atacttagga gcaaaaaatg gctaaaattt ctttggataa
     7321 atccaagatc aaattccttc tgcttgaggg cgtacatcaa aacgcaattg atgtactctt
     7381 agcggctggt tataccaata ttgaagtgca taaaaaagca ttagacggtc aagaattaat
     7441 tgatgcaatt aaagatgcac actttgttgg tattcgctcg cgtacttttt taaccgaaga
     7501 agtactggct caagctccga aattaattgc gattggttgt ttctgtatcg gtacaaacca
     7561 agttgatttg aatgcggcga aaagacgagg tattccggta ttcaatgcac cgttctcaaa
     7621 tacccgctcg gtggcagaat tagtgctggc agagatgatt ttattaatgc gtaaagtccc
     7681 gactgcaaat gcggaagtgc atcgtggggt gtggaataaa tcggcagcag gtgcaaacga
     7741 agtacgtggc aagaaattag gtattgtggg atatggtcat atcggttcac agcttagtat
     7801 tcttgccgaa gccattggta tgcaggttta tttctatgat gttgaaaaca aactaccact
     7861 cggtaatgca cagcaagtgc cgacattaga gcatttgctt tcaacctgcg atatgatttc
     7921 actacacgtt ccggaaatcg actcgactaa aaacctaatg agtgcagaac gcattgctca
     7981 attaaaagaa ggctcggtgt taattaatgc cgctcgtggt acagtggtcg atttagacgc
     8041 attagccgct cggattgaag acggctcatt gcgtggtgcg gcgattgacg tatttccgga
     8101 agaaccggca tcaattaatg acccgttcgt gtcaccattg tgcaagtttg ataacgtgat
     8161 tttaacgcca catattggtg gttcaacctc tgaagctcag gcaaatatcg gcaccgaagt
     8221 tgccaataaa tttgtgaaat attcagataa cggttcaacc gtgacggcag tgaatttccc
     8281 tgaagtatcc ttaccgcttc atactaatac caaacgttta ttacatattc acgagaatcg
     8341 cccaggtatg ttaaatcaaa ttaaccagat ttttgtggaa tcaaatgtga atattgccgc
     8401 tcaatattta caaaccgatg cagctatcgg ttatgttgta attgatgtag agagcgacaa
     8461 tgttgatgaa gcattacaac gtttaaaagc gatcgaaggc acgattcgag cccgagtatt
     8521 atattaaaag gcaaagagcc tgaatcactt caggctcttt tgtgttacaa gcggttattt
     8581 tttcagaaaa ctttaccgct aagattagaa acgataagtt gcattcgcgg tgaaagcgtt
     8641 accttttaat tttgtgccgc tacgttttaa attatcgcga gtgtattctg cacctaattc
     8701 aacatcgctt gaaactgcat attttgcacc agcaccgaaa ccaaagccac gataggtttc
     8761 attaccgatt ttgcttgagc tggcatctac ttttacataa ggtaataaat cggatccaac
     8821 acgataacct tgttgataag ctatagtata tttatttttt tgttttacat cgctaaatac
     8881 ttttgtacta cctacttttg cttttccttg tacaacgcct acaaagttat tgccttgctc
     8941 aataccataa tcgacaacta atgccggacc tgtggaatcc ttgcctttta ctccttcaac
     9001 tttgtatttt gttgtagtta catctacacc gacaccgatg ccagtaaatg tatttccgac
     9061 aggagcggct aatagatggg tagaaaataa gcaagttaat gcgacaatag ttaatttttt
     9121 cataaaatta ctcctatctt gattaaggtt cgatgttacc acagaaacgg tagccttcac
     9181 cgtgaatggt gacaataatt tctggggtat tggggtggtc ttcaaaatgt ttacgaatac
     9241 ggcgaatagt aacatcaacg gttctatcat gaggttttag ttcccgcccg gtcatttttt
     9301 tcaataaatc ttcacgtgtt tgaatttttc caggatgttg gcaaaaatgt aataaagccc
     9361 taaattcact acgtggtaat ttatttacct caccttctgg tgagattaaa ctatgactgt
     9421 taatgtctaa gatccaaccg ttaaagcgat agctttcaat ttcagattgc tcttttacaa
     9481 cgtcgttttc tttatctatc atcgttcgtt gcaataaatt tctcgctcgg atagtcaatt
     9541 ctcgaggatt aaacggttta gtgatatagt catcggcacc tatttctaaa ccgaggatct
     9601 tatcaatttc attgtcgcga ccggttaaaa acattaatgg tagcttcata ttctctcgaa
     9661 tttctcgggc gagaattaat ccattttttc ccggtaagtt aatgtccatt agcacaagat
     9721 tgatttcatt tgtactcaga acagagtgca tttcctcgcc gttagatgct tgaaatacct
     9781 gatatccttc tccttcaaaa atgcttttca gcatattcct tgttactaac tcatcctcaa
     9841 caattaagat ttgtgggatc ttcatctttt aaacctctga tttaattta
//
LOCUS       DANLYI010000078         9872 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.078, whole genome shotgun sequence.
ACCESSION   DANLYI010000078 DANLYI010000000
VERSION     DANLYI010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 9872)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9872)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9872
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.078"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            178..1884
                     /locus_tag="PXD81_001379"
     CDS             178..1884
                     /locus_tag="PXD81_001379"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009875393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HDL5537400.1"
                     /translation="MNTSSFNRTWAKVILSALMRYGVKHFCIAPGSRSTPLTLAAVQL
                     QQTQYAECHTHFDERGLGFYALGIAKATDSPVAIIVTSGTAVANLYPAVIEASLSHQK
                     LIILSADRPTELIGCGVNQAIHQQGIFANYPVASVNLPKANEAYHASWLVSTIEQACT
                     TQAQQGGVLHINVPFEEPLYEADINEILNTPWLRPIQGWLNNAQTKWIDQQAIQSEVS
                     MHENWDYWRTKRGVIVVGKLPPEQGTGLKTWAETMGWCLLSDVQSCIEASLPYADIWL
                     SNETVKQRLLQADIVIQFGTQIVSKRVNEFLKAFQGEYWVVEQSHDYHNPNAHHQTRF
                     IAKAHHFLRVHPPLRQKPWLLEPLALSQFCSTFIEQQVGGSLSEASLAHNIQDVLAIN
                     GNLFIGNSLFVRLVDALCKLPEGYPVYTNRGASGIDGLIATMAGIAKGSDRPTVGVVG
                     DISALHDLNSIALLTKINQPTILFVVNNSGGALFDMLPVDSKAKDQYYRLAHNFEFAQ
                     VATMFGLEYIRPFTWADLKTKLKHAYARKGVTIVEIKVNEHDGSNMYKSLLKQISQAE
                     IA"
     gene            1933..2772
                     /gene="menH"
                     /locus_tag="PXD81_001380"
     CDS             1933..2772
                     /gene="menH"
                     /locus_tag="PXD81_001380"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HDL5537401.1"
                     /translation="MLSYQWHAQQGTPVVFLHGLLGSQQDWQAVLQHLQNFPQIRPLT
                     IDLPFHGNSKHITCADFANLRQQLHATLESLIGSRPFYLVGYSLGGRAALDYVLNMEN
                     PNLQGVVLEGANIGLSTENDRQIRRQNDRHWANRFRQEPIEQVLEDWYKQPVFADLSD
                     TERECYIDSRKHNQGEQIAQMLEATSLAKQPDYTEKLKQTDKNIVFFIGEKDQKFRKM
                     ASSHQLPTQLIANAGHNAHRANAKNFVEKLGLLILHSDSDLRSQSVKHNGAALLHKSP
                     SGA"
     gene            complement(2821..3498)
                     /locus_tag="PXD81_001381"
                     /pseudo
     CDS             complement(2821..3498)
                     /locus_tag="PXD81_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251475.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            3584..4246
                     /locus_tag="PXD81_001382"
     CDS             3584..4246
                     /locus_tag="PXD81_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012478341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heteromeric transposase endonuclease subunit
                     TnsA"
                     /protein_id="HDL5537402.1"
                     /translation="MRKTHSFQQVRKIKPTWMSVSGHIPFRNGVSIPYESTLERDFLM
                     YFTYLPSVDEVVSQPTTLPFVKNGITYTYTPDFFLSFTDGRKPMLIEVKPKAKWQKHW
                     KEWKEKWKAAIRFCQENGYVFHVYDEDRIRHLALFNLNYVQRYKRIQHEQEDIDAILA
                     QVKLMGNTTIDYLLSRFFAGSLYRMKGLQIIYHLLATKQLHCNWFLPLNEFTEVWGNN
                     DE"
     gene            4236..6164
                     /locus_tag="PXD81_001383"
     CDS             4236..6164
                     /locus_tag="PXD81_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015586984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HDL5537403.1"
                     /translation="MMSNSGFTEKRYHHRLDRGRIILQKGNIYLNREDGEQYELVDYM
                     DEPSQLLVRNLNTRTTKVVSIHQLENFKMNERTDLSVDVTAISNEYWEKAQQKYEAIK
                     PLLGMDQHRPYAVKARAEDVGVNPRTLYRWLQAYNSIGSIAGLVDQKRGWQQGNSRLT
                     PEQDKLIVQVINEFYLHKQRPTTEQTIREIRRRCKIEKVESPSKETIRMRILHISEEE
                     RLRKRGQREKARNKFKPKPNSFPDADYPLSVVQIDHTPVDLIIVDSKYRKPIGRPFLT
                     VAIDIYSRMIVGYYLSLDAPSVTSVAMCIARGILPKERLLLDLGLQGSEWNAFGYPVK
                     VHVDNGPDFQALDLSKSCSAHGIHLEFRPMGRPEYGGHIERVIGTFMKEVHSLAGTTF
                     SNIKERDSYDSEKEAIMTLDEFEKWLVHYIVNVYHKRVHSALGISPEQKWKIGIFGDE
                     NEVGCGYPQLPVDEQTLLLDFLPSITRTIQHNGVTIDGLRYYDVALNMYISDSDESGK
                     SKEFLFRRDPRNISKIWFYDPKLKRYFPIPFANQAMPEMSIWEYREVRSRIANKGDKY
                     INEQQVLDGLTEMREMVAESAQRTKKARRQAERQKMHKASKPIIETKVETKAVVPVVV
                     TSNLLALDDESLSFGEVD"
     gene            6164..7051
                     /locus_tag="PXD81_001384"
     CDS             6164..7051
                     /locus_tag="PXD81_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012478339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TniB family NTP-binding protein"
                     /protein_id="HDL5537404.1"
                     /translation="MQYEHVHEKFRHLVTADNQERIAFLDEPRWLGYGVAKDIMDNLV
                     SLMNKPKRPRMLNLLIVGDSNNGKTTLIRRFFDLYGQAYIDSDSNAIYPILLAEAPPS
                     ANEKELYISLLERFYVPYKPTDTIAKLRYQTIHLFREFRVKMLVIDEFHSLLVGTPRL
                     QRQVMNAIKMLCNELQIPIVGVGTRDAIRVLHTDRQHASRFDVAELPIWKLDKEFQKL
                     LFQFQGILPLRESSNLQSPELATKIHTISGGNLGNVHRLLTACAVQAITSGTEKITLD
                     IIEKNSWVQPTNGYRKIIG"
     gene            7009..8115
                     /locus_tag="PXD81_001385"
     CDS             7009..8115
                     /locus_tag="PXD81_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TniQ family protein"
                     /protein_id="HDL5537405.1"
                     /translation="MGTTNKRLSQNNRIKKGVIHTPIYSNESISSWLIRASFDCGTDP
                     LTFTGFYWEKHRLWTLDLDRGLENIEPTIYDDVQALSGKIQLSEYSLFRRLYPINADE
                     TLAKGQVRWVIPRSARNRTHHIGQQYCPLCLDESPHLRNDWRLAWNFGCLKHNMMLAN
                     RCFCCGTLYQPHLLSAEKRHLNHCHQCGGKLEQGTMEMSLAEKLTLQGINQVLEKDSG
                     ICFGYKVSGKVWFDMLRYCINLLRRASDRPNHALAKFVVNLQIDVENICSPKTGLSFE
                     QLPLGERTNLLVNAYHILGMEADSIRSAIYLCGGTQKTFMFEKYPDFWQEFMALAPAG
                     PNRTRKRTKPQNESITAINRRWERLKRKLHLNNE"
     gene            8112..9536
                     /locus_tag="PXD81_001386"
     CDS             8112..9536
                     /locus_tag="PXD81_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5537406.1"
                     /translation="MKINVIKPQEEPCSACGKISRIWRRYNGEGYCANCYAKWFPKKE
                     CSCCHQPKRIYLSSTVCWDCEKQTDCVRCGKAAGTFKIGKITRYGAVCNVCSRYFREE
                     RECFECGQLSRTMLRASESGHSELLCLKCFQKYRFATCKNCRRYRKVHDRDNQLCRKC
                     SEQRTSQCPKCKQDMPAGYGNICADCSRRTLLFNLIRMNVHLFRHKKVKRAYKKFIFW
                     YLAKCGVSVVLKKSDDHVLFFMRCDENWGNIPDYRELVTFFKPNGLRTHLTVLRWLLD
                     TNQVKVDESLKEEMAGLERIQSLFNKLSNSVPCIANYYTQLQHKYEAGKTSLKSVRLA
                     LQPAIDFISSESVSLLPTQVQLNSYLVAKTGQIAAITGFINHLNREYHCELEIDRSLI
                     QKMKEKQLKQQAKQRLAILYKQEKLSIKEQNELLSVVLLNLHNIWIKSPKKLSISEID
                     GIAYYVDNEKKYFIPKDIHIRLME"
     gene            9651..9857
                     /locus_tag="PXD81_001387"
     CDS             9651..9857
                     /locus_tag="PXD81_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA delta(2)-isopentenylpyrophosphate
                     transferase"
                     /protein_id="HDL5537407.1"
                     /translation="MTLKAQFREWLKKEQEHDEMIGFAHIPCILSKVDSKLAIVSGNA
                     YEKKYLRYWIKCFRFDRNISKYIK"
BASE COUNT         3054 a         1990 c         2128 g         2700 t
ORIGIN      
        1 cgtaaatgct cttaaaaaag agagagttta ctaagtaaag tgttgctcaa tagatagaaa
       61 ttttcacgaa gttttacaaa aacattccaa ttttctccta aaaaacttcc cacaataatt
      121 tcactttttg ctacaatcct aaccatttat cgaatttatc aatcagtaga gaagaatatg
      181 aataccagtt cttttaatcg cacttgggca aaagtgattt taagtgcatt aatgcgttat
      241 ggcgtaaaac atttttgtat tgctccgggt tctcgctcaa caccgctcac attagcggca
      301 gtgcaacttc aacaaacgca atatgcagag tgccacactc attttgatga gcgtggttta
      361 ggtttttatg cgttagggat tgctaaagca accgacagcc cggttgctat tattgtcact
      421 tccggcacag cagtagcgaa cctttatcct gcggtgatag aggcaagcct gagccaccaa
      481 aaactgatta ttctttctgc cgaccgccca accgaattaa tcggctgtgg ggtaaaccaa
      541 gccattcatc agcaaggtat ttttgccaat tacccggttg ccagtgtcaa tctaccgaaa
      601 gcgaacgagg cttaccacgc aagctggctg gtttcgacca ttgagcaagc ctgcactacc
      661 caagcacaac aaggcggtgt gttgcatatc aatgtgcctt ttgaagagcc gctttacgaa
      721 gcagacatta atgaaattct aaatacacct tggttacgcc caatccaagg ctggttgaat
      781 aacgctcaaa ccaaatggat agaccaacaa gcgattcaaa gcgaagtctc aatgcacgaa
      841 aattgggatt actggcgaac taaacgaggg gtgattgtgg tcggcaaact gccgccggaa
      901 caaggcacag gtttaaaaac ttgggcggaa accatgggtt ggtgcttgct aagtgatgtg
      961 caatcttgca ttgaagccag cctgccgtat gccgatattt ggctctccaa cgagactgtc
     1021 aaacagcgtt tattgcaagc ggatatcgtg attcaattcg gcacgcaaat tgtcagcaaa
     1081 cgggtgaatg aatttttaaa agcattccaa ggggaatatt gggtggtgga acaatcgcac
     1141 gattaccata atccaaacgc tcatcatcaa acccgtttta ttgccaaagc acatcacttt
     1201 ttgcgggtac acccaccact tcgccaaaag ccatggttgc ttgagccatt ggcattatcg
     1261 caattctgct ccactttcat agagcaacaa gtgggtggca gcttaagtga agcctcactt
     1321 gcacacaaca ttcaagatgt tttagcaatt aatggcaact tatttatcgg caatagctta
     1381 tttgttcgat tggttgatgc tctgtgcaaa ctgccggaag gctaccctgt ttacaccaac
     1441 cgtggtgcaa gcggtattga tggcttaatc gcaacaatgg caggcattgc aaaaggtagc
     1501 gaccgcccga ctgtcggtgt ggtaggtgat atttccgcat tacacgatct taactcaatt
     1561 gcattgctca ccaaaattaa ccagccgacc attttatttg tggtgaataa cagcggtggt
     1621 gcgttgtttg atatgttgcc tgttgatagc aaagcgaaag atcaatatta ccgccttgct
     1681 cacaactttg aatttgctca agtcgccaca atgttcgggc ttgaatatat ccgcccgttt
     1741 acttgggcag atctaaaaac caaactcaaa cacgcctatg ctcgtaaagg tgttaccatt
     1801 gtggaaatca aagtaaatga acacgacggc agcaatatgt ataaatcatt gctaaaacaa
     1861 atttcacagg cggaaattgc ctaatcacaa ctgcccctcg ccaatcctgc gaggggtaaa
     1921 aatgtatcga ctatgctttc ctatcaatgg cacgctcaac aaggcacacc cgtggttttc
     1981 cttcacggct tgctcggctc acaacaagac tggcaagctg ttctccaaca tttgcaaaat
     2041 tttccgcaaa ttcgaccgct taccattgac cttccttttc acggaaatag taagcacatt
     2101 acctgtgccg attttgccaa cctgcgacaa caactacacg ccacccttga aagcctaatc
     2161 ggctcacgac ctttctattt agtcggctac tcactcggtg gacgagcggc actcgattat
     2221 gtgttaaata tggagaatcc gaatctgcag ggcgtggtgt tagaaggggc gaatatcggc
     2281 ttaagcacag aaaacgaccg ccagattcgc cggcaaaatg accgccattg ggcaaaccgt
     2341 ttccgtcaag aacccatcga acaagtgctt gaagattggt acaaacagcc tgtttttgcg
     2401 gatttaagcg atacggaaag agaatgttat attgatagca gaaagcacaa tcaaggagag
     2461 caaattgctc aaatgctgga agccaccagc ctcgccaaac agcctgacta caccgaaaaa
     2521 ctcaagcaga cggataagaa tattgtcttt tttatcggtg aaaaggatca gaaatttcgc
     2581 aaaatggcat caagccacca actaccaaca caactgattg ctaatgcagg gcataacgct
     2641 catcgggcta atgctaaaaa ttttgtggag aaattggggt tattgatact ccactccgac
     2701 agcgacctac ggtcgcaatc ggttaagcat aacggtgcag ctctgctgca caaaagcccc
     2761 agtggggctt aatcagccgt aggctaaata taacctagta cggctcagcc gtgctaggtg
     2821 ttagggctcc ccctgcaacc gttgcaataa gcgatccatc gcccgataac caagtgcttc
     2881 cgctaaatgc agttgttgaa tattcgcctc gccggctaaa tcagcaatag tccgagaaac
     2941 tttcaaaata cggtggtagg ctcgtaccga taaaccgagc tttgtcaatg cagtttctaa
     3001 aaatagggca tcttgtgccg ataattggca atctcgctca atttctcggg tggtgagttg
     3061 agaattgatt ttacctgctc gctcaaattg catttttctg gctaaaaaca ctcgcttgcg
     3121 aacctgctcg gtggtctctc ctcgattctc cgtcccactt tgcaatgctc cttgtggcaa
     3181 aagtggcact tctatagaga gatcgaaacg gtctaagaaa ggtccggaaa ggcggtttaa
     3241 ataccgcatt acctgttggg gcgaagtgcg gttatgcgtg ccttgataat gccctgtcgg
     3301 gcttgggttc atcgctgcaa ttaattgaaa gcgtgccgga aattgtactt tagcgttggc
     3361 tcgggaaatc acaatttcgc ctgcctccaa cggctgacgt aacgcatcaa gcactttacg
     3421 ttcaaattca ggcaattcat ctaagaacag cacaccgtta tgggcaagtg aaatttcccc
     3481 cggtttgggg atcgagccac tgtaaaaact agaaataaat gtcataaaaa ctagaaatag
     3541 ctgtcagatt atttagaaaa caagaaatag gtgtcaaatt attatgagaa aaacacatag
     3601 ttttcaacag gttagaaaaa ttaaacccac ttggatgagt gtatcggggc atattccatt
     3661 tagaaacgga gtcagtattc cctatgaatc gactttagag cgtgattttt tgatgtattt
     3721 cacttatcta ccttcagtag atgaagtcgt ctctcaaccg actaccctac ctttcgtcaa
     3781 gaatgggatc acttatacct atacaccaga tttcttcttg tcatttacag acggtcgaaa
     3841 gccgatgtta atcgaggtca aaccgaaagc aaaatggcag aagcactgga aagaatggaa
     3901 agaaaaatgg aaagctgcaa tacgtttttg ccaagaaaac ggttatgtat tccatgtgta
     3961 tgatgaagat cggattcgac atctagcgct tttcaatctc aactatgttc agcgctataa
     4021 gcgaatacaa catgaacaag aagatataga tgctattcta gcccaagtaa agctgatggg
     4081 aaataccact attgactatt tactctctcg attttttgca ggttctctat atcgcatgaa
     4141 aggcctacaa atcatctatc acttactggc aacgaaacaa ctacattgca attggtttct
     4201 accacttaat gaatttaccg aggtatgggg gaataatgat gagtaactct ggatttacag
     4261 aaaagcgcta tcatcaccga ttagatcggg gacgtattat tttgcaaaaa ggtaatattt
     4321 acctaaatag agaagatgga gaacagtatg aattggttga ttatatggat gaaccctccc
     4381 aactgctcgt acgaaatctc aacactcgta caaccaaagt cgtcagtatt catcagcttg
     4441 aaaatttcaa aatgaacgag cgaacggatc tttctgtgga tgtaaccgct attagtaatg
     4501 aatattggga gaaagcccaa caaaaatatg aggcaattaa accgttatta gggatggatc
     4561 aacatcggcc ttacgcagtt aaggctcgtg cagaagatgt tggggtgaac cctcgtacat
     4621 tataccgttg gttacaagcc tataattcca taggatctat cgcaggttta gtcgatcaaa
     4681 aacgtggatg gcaacaagga aattcccgtt taacgccaga gcaagacaag ctgatcgtgc
     4741 aggtaatcaa tgagttttac ctacataaac aacgcccaac tactgagcaa actattagag
     4801 aaattcgccg ccgatgcaaa attgaaaaag tggaaagccc aagcaaagaa accatccgta
     4861 tgcgcatttt gcatatttca gaggaagagc gattacggaa acgcgggcaa cgagaaaagg
     4921 cacgcaacaa atttaagccg aagccaaata gctttcctga tgcggattat ccattgagtg
     4981 tggtacaaat tgaccataca ccagttgacc taattattgt ggatagtaag tatcgtaaac
     5041 cgattggccg accgtttttg acggttgcga tagatatata cagcagaatg attgttggtt
     5101 attacctttc acttgatgcg ccttctgtaa cttcggtggc gatgtgtatt gcacgtggta
     5161 ttttgccgaa agaacgccta ctgcttgatt taggtttgca aggctcagaa tggaatgctt
     5221 ttggctaccc agtgaaagtg catgtggata atggcccaga ttttcaggct ttggatttat
     5281 ctaaatcttg ttctgcacac ggaatccatc ttgaatttag accgatgggg cgacctgaat
     5341 atggcggaca tattgagcgt gttatcggta catttatgaa agaagttcac agtttagcag
     5401 gaacaacatt ctccaacatc aaagagcgtg actcttacga ttcggaaaaa gaggccatta
     5461 tgactctcga tgagtttgaa aaatggttgg tacattacat tgtgaatgtt taccacaaac
     5521 gtgtgcattc tgctttaggc atttctcctg aacaaaaatg gaagattggt atttttggag
     5581 atgaaaatga agtgggatgt ggttatccgc aacttcctgt tgatgaacaa acactactac
     5641 ttgatttctt gccaagtatc actcgtacca ttcagcataa tggtgtaaca attgacggac
     5701 ttcgttatta cgatgttgcg ctaaatatgt atatcagtga ttcagatgaa agcggtaaat
     5761 ctaaggaatt tctgtttcgc cgagacccgc gaaatatcag caaaatttgg ttttatgatc
     5821 cgaaattaaa acgttacttc ccaattccat ttgcaaatca ggcaatgccc gaaatgagca
     5881 tttgggaata tagagaggtt cgtagccgaa ttgcgaataa aggtgataaa tatatcaacg
     5941 agcaacaagt tttagacggt ttaaccgaga tgcgtgaaat ggttgctgaa tcggctcaac
     6001 ggacgaaaaa agcacgccgt caggctgaac gacaaaaaat gcacaaagca agtaagccaa
     6061 ttattgaaac aaaagtggaa actaaggctg ttgtaccagt cgtagtaaca tcgaatttgc
     6121 ttgctttaga tgatgagagc ttatcttttg gtgaggtgga ctaatgcaat acgaacatgt
     6181 tcatgaaaaa ttccgccatc ttgtgacagc ggacaatcag gagaggattg cgttcttgga
     6241 cgagccaaga tggttaggtt atggtgtcgc caaagacatt atggataatt tggtttcttt
     6301 aatgaataaa ccaaaacgcc cacgaatgtt gaatttgttg attgtcggcg attccaataa
     6361 tggaaaaacc acactgatcc gccgcttctt cgacttgtac ggtcaggcgt atatcgacag
     6421 tgattctaat gcgatttacc ctattcttct tgccgaagcc ccaccaagtg cgaatgaaaa
     6481 agaattgtac atctcattat tagagcggtt ctacgtacca tataagccga cagatacgat
     6541 agccaaactt cgttatcaga ctatccattt gttccgagaa tttcgtgtca aaatgttggt
     6601 tattgacgag tttcattctc tgttggttgg tacaccacgc ttacaacgac aggtgatgaa
     6661 cgcaattaag atgctttgta acgagctaca aattccaatt gtcggtgttg gtactcgtga
     6721 tgcaattcgg gtattacata cggatcgaca acatgccagt cgttttgatg ttgcggaatt
     6781 gccaatatgg aaactagata aggaatttca aaaactccta tttcaattcc aagggatttt
     6841 acctttgagg gagagttcaa atctgcaatc tccagaactt gctacgaaga tccacaccat
     6901 ttcaggtggg aacttaggga acgtgcatcg cttactaaca gcttgtgcag ttcaagccat
     6961 tacaagtggc acggaaaaaa tcaccttaga catcattgag aaaaattcat gggtacaacc
     7021 aacaaacggc tatcgcaaaa taatcggata aaaaaaggtg ttattcatac ccctatttat
     7081 tcaaatgaaa gtattagctc gtggttaatt cgtgcatcct ttgattgtgg tacagatcca
     7141 ctgactttta cagggtttta ttgggaaaaa catcggttgt ggacattaga tttagatcgt
     7201 ggcttggaaa atattgagcc aactatctat gatgatgtac aggcattatc gggtaaaatc
     7261 caactttcag aatattcctt gtttcgcagg ctttacccta taaatgcaga tgaaacctta
     7321 gctaaaggtc aagtaagatg ggtgattcca cgttcggcaa ggaatagaac ccatcacatt
     7381 ggtcaacaat attgtccttt gtgcttggat gagtcgccac atttacgcaa tgattggcgg
     7441 ttagcgtgga attttggttg tctcaagcat aatatgatgt tggcgaacag atgtttttgt
     7501 tgtggtacat tgtatcaacc ccatttacta tcagcggaaa agaggcattt gaatcattgt
     7561 catcaatgtg gtgggaaact ggaacaaggc acaatggaaa tgagtctagc tgaaaagctg
     7621 acattgcaag gtattaatca ggtactcgaa aaagattcag gcatctgttt tggatacaag
     7681 gtgtcgggta aggtgtggtt tgatatgctc cgctactgca tcaacttatt gagacgtgca
     7741 tcagacagac caaatcatgc cttagcaaaa tttgtagtta atctacagat tgatgttgaa
     7801 aatatatgta gccccaaaac aggactttct tttgaacaac ttccgctagg tgaacgtaca
     7861 aatttacttg tgaatgccta tcatattttg ggaatggaag cagattctat tcgtagtgca
     7921 atttatttgt gtggcggaac acaaaaaacc tttatgtttg aaaaatatcc tgatttttgg
     7981 caagaattta tggcactagc acccgcaggc ccaaatagaa cacgaaaacg caccaagccc
     8041 caaaatgagt ctatcacagc aattaatcgc cgttgggagc gtttgaaacg caaactacac
     8101 ttaaataacg aatgaaaatt aatgtaataa aaccacagga agaaccttgc tccgcttgtg
     8161 gcaaaatttc tcgcatttgg cgcagatata atggcgaagg ctactgtgcc aattgctatg
     8221 ctaagtggtt tccgaaaaaa gagtgcagct gttgccatca accaaagcgt atctatttga
     8281 gttcaacagt atgttgggat tgcgaaaaac agactgactg tgtacgttgc ggtaaggctg
     8341 cgggtacatt caaaattggt aaaattaccc gttatggtgc agtttgtaat gtgtgttcac
     8401 gttattttcg tgaagaacga gaatgttttg agtgtggtca actatctaga actatgcttc
     8461 gagcatcaga gtcaggtcat agcgagctac tctgcttgaa gtgttttcaa aaatatcggt
     8521 ttgcgacttg taaaaactgt cgccgttatc gcaaagttca tgaccgtgat aatcagcttt
     8581 gtcgaaaatg tagtgagcaa cgaacttcac aatgcccgaa gtgcaaacag gatatgcctg
     8641 cgggatatgg taatatttgt gcagattgtt ctaggcgaac tctactgttc aaccttattc
     8701 gaatgaatgt gcatttattc cgccacaaaa aagtgaaaag agcatataaa aagttcattt
     8761 tttggtatct cgcaaagtgt ggcgtgagtg ttgtgctgaa aaaatcagac gaccacgtcc
     8821 tcttttttat gcgttgcgat gaaaattggg gcaacattcc agactaccgt gagttggtta
     8881 cattctttaa acccaatgga ttacggacgc atttgactgt gttgcgttgg ttgcttgata
     8941 ccaatcaagt caaagtagat gagagcttaa aagaggaaat ggcggggttg gaacgtattc
     9001 aatccctatt taacaaatta agtaactctg taccttgtat tgcaaactac tacacgcagt
     9061 tacaacataa atatgaggcg ggcaaaacat cactgaaatc tgttcgatta gctttacaac
     9121 ctgcgattga tttcatcagt tcagaatctg tctcgttact gccgactcaa gtacaactga
     9181 atagctatct tgtggcaaaa acgggacaga ttgctgctat tacaggcttt ataaaccacc
     9241 tgaaccgaga atatcattgt gaattagaaa ttgaccgctc cctcattcaa aagatgaaag
     9301 aaaagcagtt gaagcagcaa gcaaaacagc gattagcgat actctacaag caagaaaaac
     9361 taagtatcaa agagcaaaat gagctactct cagtcgtatt actgaatttg cacaacattt
     9421 ggataaaatc accgaaaaaa ttaagtattt cagagataga cggtatagcc tactatgttg
     9481 ataatgagaa aaaatacttt ataccaaaag acattcacat acgcctgatg gaataagctg
     9541 tcgtcaattt ttatttttgc gatctgtgtc gagaaaataa tatttatgaa tcgacagatg
     9601 aaataatatt gataaagtct ctttgtgtgt tcaacaaaca ggagataaat atgacattaa
     9661 aggctcaatt tcgtgaatgg cttaaaaaag agcaagaaca tgatgaaatg atagggttcg
     9721 cccatatccc ttgcatttta tctaaagtgg atagcaaact ggcgattgtt agcggaaatg
     9781 cttatgagaa gaaatatctc agatattgga tcaagtgttt taggttcgat agaaatatca
     9841 gcaagtatat taaataggtt aaatatctag ct
//
LOCUS       DANLYI010000079         9867 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.079, whole genome shotgun sequence.
ACCESSION   DANLYI010000079 DANLYI010000000
VERSION     DANLYI010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 9867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9867
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.079"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            56..1543
                     /locus_tag="PXD81_001388"
     CDS             56..1543
                     /locus_tag="PXD81_001388"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HDL5537408.1"
                     /translation="MNAENSCIVIFGASGDLTFRKLIPALYNLYKIGRLGEHFSVLGV
                     SRSELTDESFRQKMRDALVKFEKASGEELDKFCEHLYYQAVNTSDAVDYAKLLPRLDE
                     LHDKYQTAGNTLYYLSTPPSLYGVIPECLAAHGLTTEEFGWKRIIVEKPFGYDIETAK
                     KLDVQIHKCFEEHQIYRIDHYLGKETVQNLLVLRFSNGLFEPLWNRNFIDYVEITGAE
                     SIGVEDRGGYYDGSGAMRDMFQNHLLQVLAMVAMEPPAIINANSMRDEVAKVLYCLHP
                     LTEEDVKHNVVLGQYARGTVDGKEVPAYLEEKGVPADSNTETFMAVKCKIDNWRWAGV
                     PFYVRTGKRLPTRVTEIVIHFKTTPHPVFSQNAPENKLIIRVQPDEGISMRFGLKKPG
                     AGFEAKEVSMDFRYSDLSSSSSLLTAYERLLLDALKGDATLFARTDAVHACWKFVQPI
                     LAYKANRGRVYEYESGTWGPTEADKLIARHGKVWRKPSGTMKKKV"
     gene            1683..2381
                     /gene="pgl"
                     /locus_tag="PXD81_001389"
     CDS             1683..2381
                     /gene="pgl"
                     /locus_tag="PXD81_001389"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005620404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HDL5537409.1"
                     /translation="MNKVIFPTAQAAVEQIAKEFIEYSHQNRPVHISLSGGSTPKLLF
                     KTLAQAPFNTEIQWQNLHFWWGDDRMVAPTDPESNYGEVQKLLFDHIDIPSENIHRIR
                     GEENVEQELARFSAELTACVPNLAFDWIILGMGTDGHTASLFPHQTDFNDQNVAVIAK
                     YPETGQIRISKTASLIEKAKRITYLVTGASKAEILKEIQTTPAENLPYPAAKIQAENG
                     VTEWYLDSEAANLL"
     gene            2557..3444
                     /locus_tag="PXD81_001390"
     CDS             2557..3444
                     /locus_tag="PXD81_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HDL5537410.1"
                     /translation="MNPIQLIEPNINSKIFTIRGEQVMLDVHLAELYQVDTRTFNQAV
                     KRNEERFPDIFRFQLTENEWLSLRSQFVILNENSGRGQHRKYLPYVFTEQGIAMLSAV
                     LRSEVAVQVSIKIMQAFVQMRHLLQTNSGLVQRLESVERKQLEMSGQLEQVFQSLEGN
                     KPKMQGVFFEGETFNAYQFVTELVRGAKSSLILIDNYVDDTVLTLFTKRSHNVSVTIY
                     TKQISKQLALDLEKYNSQYAHINVKIFDKSHERFLIIDEKELYHIGASLKDLGKKWFA
                     FSKMDLSMSRILEKLAENQ"
     gene            3454..4143
                     /locus_tag="PXD81_001391"
     CDS             3454..4143
                     /locus_tag="PXD81_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HDL5537411.1"
                     /translation="MKTLGILGGMSAESTVSYYLDINRSVNKVLGGNSSAKILISSVN
                     FEEIVQCQKQGNWQAAAEILSEQARLLEQAGAEGILLATNTMHKIADQISSHISVPFL
                     HILDCVAEAISAQGLQKVALLGTKFTMSDNFYKDGLLERGITSIVPDEETQQEIHRII
                     FEELCLGKITESAKAFYLQTIQQLSEQGAEGVILGCTEIGLLINQNDNPLPFFDTAAL
                     HSQMAVEFILG"
     gene            4226..5680
                     /gene="gnd"
                     /locus_tag="PXD81_001392"
     CDS             4226..5680
                     /gene="gnd"
                     /locus_tag="PXD81_001392"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005714317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating NADP(+)-dependent
                     phosphogluconate dehydrogenase"
                     /protein_id="HDL5537412.1"
                     /translation="MSQKGDIGVIGLAVMGQNLILNMNDNGFKVVAYNRTTSKVDEFL
                     EGAAKGTNIIGAYSLEDLAAKLEKPRKVMLMVRAGEVVDQFIEALLPHLEEGDIIIDG
                     GNSNYPDTNRRVKALAEKGIRFIGSGVSGGEEGARHGPSIMPGGDESAWQYVKPIFQA
                     ISAKTDKGEPCCDWVGRDGAGHFVKMVHNGIEYGDMQLICEAYQFLKDGLGLSYDEMH
                     EIFKEWKNTELDSYLVDITTDILAYKDEDGEPLVEKILDTAGQKGTGKWTGINALDFG
                     IPLTLITESVFARCVSAFKDQRVAASRLFNKEIGKVEGDKKVWVEAVRRALLASKIIS
                     YAQGFMLIREASEQFGWDINYGNTALLWREGCIIRSRFLGNIRDAYEANPYLVFLGSD
                     PYFKDILENCLADWRKVVAKSVEIGLPVPCMASAITFLDGYTSARLPANLLQAQRDYF
                     GAHTYERTDKARGEFFHTNWTGRGGNTASTTYDV"
     gene            5815..6408
                     /locus_tag="PXD81_001393"
     CDS             5815..6408
                     /locus_tag="PXD81_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase"
                     /protein_id="HDL5537413.1"
                     /translation="MLTDQDIFNRYQTKKSEFEKQADISLIGHSLFDMWGDMENGTPN
                     LAGKTVANLGLSGTSTRQYLDVITRQERIQHVGDNVFIFLGVNDIVKEPDYSPKQVLE
                     WIGEIVDSLRKINPNANFFLLEATPVNNITTTNNAAIKELNAYFEANIPSDLTYVKTW
                     EYFADKEGNLDLTLCHDGLHFTQAGYDRLEAILTKHL"
     gene            complement(6479..9343)
                     /locus_tag="PXD81_001394"
     CDS             complement(6479..9343)
                     /locus_tag="PXD81_001394"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012771641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HDL5537414.1"
                     /translation="MTKQFTMEDRFNPSAVEQALYQHWESQGYFKPSEDINAPSYCIA
                     IPPPNVTGSLHMGHAFQQTLMDTLIRFNRMEGNNTLWQTGTDHAGIATQMVVERKIAA
                     EEGKTRHDYGREAFINKIWDWKAYSGGTISQQMRRLGNSIDWDRERFTMDEGLSNAVK
                     EVFVRLHEEGLIYRGKRLVNWDPKLHTAISDLEVENKESKGSLWHFRYPLANGAKTAD
                     GLDYLVVATTRPETMLGDTAVAVHPEDERYQALIGKSVILPLANREIPIIADEYVDRE
                     FGTGVVKITPAHDFNDYEVGKRHGLPMVNVMTLNADIRAEAEIIGTDGKPLSDYTAPI
                     PADYQGLERFAARKKIVADFEALGLLDQIKPHDLKVPYGDRGGVPIEPMLTDQWYVSV
                     KPLAEVATKAVEDGEIQFVPKQYENLYFSWMRDIQDWCISRQLWWGHRIPAWYDEQGN
                     VYVARDEAEVRAKHNLPADLPLKQDEDVLDTWFSSGLWTFSTLGWPEQTKELKMFHPT
                     DVLITGFDIIFFWVARMIMFTMHFIKDENGKPQVPFKTVYVTGLIRDEQGQKMSKSKG
                     NVLDPIDMIDGISLDDLLEKRTGNMMQPQLAEKIAKATRKEFAEGIVAHGTDALRFTL
                     TALASNGRDINWDMKRLEGYRNFCNKLWNASRYVLTNDKLDLSEGDVEFSLADRWIES
                     QFNRTVETFRTALSQYRFDLVANAIYEFTWDQFCDWYLELTKPIFFKGTDVQRRGASR
                     TLVNVLEKLLRLIHPVMPFITEEIWQKVKGFVGIEADTIMLQKFPQFDPLAIDETAES
                     QINFIKEVIVAVRNIRAESNIAPSKGLDLIARNFSADEVSILNANEVLLKSMAKLDSV
                     KVLENGENAPLSVAKLVANGEILIPMAGFINKEAELARLTKEMDKLKGEVARIEGKLS
                     NEAFVAKAPEQVIAKEREKMQEYLSGLEKLQVQYQEIEAL"
     gene            complement(9460..>9867)
                     /locus_tag="PXD81_001395"
     CDS             complement(9460..>9867)
                     /locus_tag="PXD81_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5537415.1"
                     /translation="IEFMVNKDDNSILGEMTSTALCELIPLTWSFRLESPKPTLLNLI
                     FDRELKLSYLVSGGYTELSTFKIKEINSNLGTLTLTTQEDKTGIFPPTIKLINFSSNI
                     EDDIKEIEQSCLNSRKQLWEKRRKMQEEQKIPN"
BASE COUNT         2826 a         2042 c         2149 g         2850 t
ORIGIN      
        1 tttatgataa tcttgcaccc aacaaaattt taacaattag caataggata aataaatgaa
       61 tgctgaaaat agctgtatcg tcatttttgg tgcttccggt gacttaactt ttcgtaaatt
      121 aattcctgct ctttataact tatataagat tggcagattg ggcgagcatt tttccgtcct
      181 aggtgtttct cgttcagaat taaccgacga atctttccgc caaaaaatgc gtgatgcgtt
      241 agtcaaattt gaaaaagcaa gcggtgaaga attagataaa ttttgcgaac atctttatta
      301 ccaagctgta aatacctctg atgcagtgga ttatgccaag ttgctgcctc gtcttgatga
      361 gctacacgat aaatatcaaa cagccggtaa cacgctttat tacctctcca cgccaccaag
      421 tttatatggc gtgattccgg aatgtttagc cgctcacggc ttaactaccg aagagtttgg
      481 ctggaaacgg attattgttg aaaaaccatt cggctacgat atcgaaaccg ccaaaaaatt
      541 agacgtacaa atccacaaat gctttgaaga gcatcaaatc taccgtatcg accactattt
      601 aggtaaagaa acggtacaaa acttactcgt tttacgtttt tctaacggct tatttgaacc
      661 gctttggaac cgtaatttta ttgattatgt ggaaattacc ggggcagaat ccatcggcgt
      721 tgaagatcgt ggcggttact atgatggctc gggggcaatg cgtgatatgt tccaaaacca
      781 cttattgcaa gtattagcca tggttgcaat ggagccaccg gcaattatta atgctaattc
      841 aatgcgtgat gaagtcgcaa aagtgctata ttgcttgcat ccgttaactg aagaagacgt
      901 taaacacaat gtggtgttag gtcagtatgc tcgtggcacg gtagacggca aagaagtgcc
      961 ggcttacttg gaagaaaaag gcgtgccggc tgattccaac actgaaacct ttatggcggt
     1021 aaaatgtaaa atcgataact ggcgttgggc aggtgtgccg ttctatgtgc gaaccggtaa
     1081 acgcttacca acccgtgtga cggaaatcgt aattcatttt aaaactacac cacacccggt
     1141 atttagccaa aatgctccgg aaaataaatt gattattcgt gtgcaaccgg acgaaggcat
     1201 ttcaatgcgt ttcggcttga aaaaaccggg agcaggcttt gaagcgaaag aagtctcaat
     1261 ggatttccgc tattctgatt taagttcatc atcaagctta ctcaccgctt atgaacgctt
     1321 actattagac gcacttaaag gtgatgcaac cttatttgcc cgtactgatg ctgtacacgc
     1381 ttgttggaaa tttgtgcaac cgattttagc ctacaaagct aatcgtgggc gtgtgtatga
     1441 atatgaatcc ggcacttggg ggccaaccga ggcagataaa ttaatcgcca gacacggcaa
     1501 agtatggcga aaaccgtccg gtacaatgaa gaagaaagtg taaatttcag ttcccctctt
     1561 tagcaaagag gggctagggg agatttggca gaagttatat acgctcatac gaaatattga
     1621 aatcgtaatc aaatctcccc ctcccctctt ttctaaagag gggtgatcta acgaggcaaa
     1681 ttatgaataa agttattttc cccaccgccc aagctgcggt tgaacaaatt gctaaagagt
     1741 ttattgaata cagccatcaa aaccgcccgg tgcatatttc cctttccggt ggttcaaccc
     1801 ccaaattatt attcaaaacc ttagctcaag caccgtttaa taccgaaatt caatggcaaa
     1861 acctgcattt ctggtggggg gatgatcgaa tggttgcccc aaccgatccc gaaagtaact
     1921 acggcgaagt gcagaaatta ctgttcgatc acatcgacat tccaagcgaa aatattcacc
     1981 gcattcgtgg tgaagagaat gtagaacaag aacttgcaag attttcggca gaattgaccg
     2041 cttgtgtgcc gaacttggcg tttgattgga ttattttagg aatgggaaca gacgggcata
     2101 ccgcctcact gttcccacat caaacagatt ttaatgatca gaacgttgcc gtgatcgcca
     2161 aatacccaga aacagggcag atccgcattt ccaaaacggc atctttaatt gaaaaagcca
     2221 aacggatcac ttacttggtc acaggagcaa gcaaagcgga aattttaaaa gaaatccaaa
     2281 ccactccggc agaaaattta ccttatcctg ccgccaaaat tcaggcggaa aatggggtaa
     2341 cggaatggta tttggatagt gaggcggcta acttactcta ggtcacaatc agtgaccttt
     2401 acaagcggtt aaattttaga aaaatgttgc aaagtatttt tgtgatattc atcgaaaaag
     2461 cagaaaaatc tgctcgtggt ataggccaaa aattggcata gtctttctta ttttgagatc
     2521 acaaaagtga tcttcactga agaataagga agaaagatga atccaattca attaattgaa
     2581 ccaaacatta actctaaaat ttttactatt cgtggcgagc aagtaatgct agatgttcat
     2641 ttggcagagc tttatcaggt agatactcga acctttaacc aagcagtaaa acggaatgaa
     2701 gagcgatttc ctgacatatt tcgttttcaa ttaacagaaa acgaatggct atctttaaga
     2761 tcacaatttg tgatcttaaa cgaaaatagt gggcgtggcc aacataggaa atatcttcct
     2821 tatgtcttta ccgagcaagg cattgcaatg ctttcggcag tattgcgtag tgaagttgcg
     2881 gtgcaagtta gcattaagat tatgcaagcc tttgtgcaaa tgcgtcatct tttgcaaaca
     2941 aatagtggtt tggtacaacg cttagaaagt gtagagcgta aacagttgga gatgtctggg
     3001 cagttagagc aggtgtttca atctcttgaa ggcaataagc ctaaaatgca aggggttttc
     3061 tttgaaggtg aaacctttaa cgcttatcag tttgttacgg aattagtgag aggtgcaaaa
     3121 tcatcgctga ttttaattga taactatgta gatgataccg ttttaacttt atttacgaaa
     3181 cgatcgcata acgtatcagt tacgatttat acgaaacaaa tatctaagca acttgcatta
     3241 gatttggaaa aatataacag ccaatatgct cacattaatg tgaaaatatt tgataaatct
     3301 cacgagcgat ttttgattat tgatgaaaaa gagctttatc atatcggtgc atcacttaaa
     3361 gatttaggta agaaatggtt cgctttttct aaaatggatt tatcaatgag ccgcatttta
     3421 gaaaagttag cggaaaatca ataaggtata ttaatgaaaa cactcggcat tttaggtgga
     3481 atgagtgcgg aaagcacggt ttcttattat ttagatatta atcgctcggt gaataaagta
     3541 ttaggtggaa atagcagtgc caagatttta atttcgagtg tcaattttga agagattgta
     3601 caatgccaaa agcagggaaa ttggcaagca gccgctgaaa ttttatctga acaagctaga
     3661 ttgctagaac aagccggagc agaaggcatt ttattggcaa ccaacacaat gcacaaaatt
     3721 gccgatcaaa ttagtagcca tatctctgtg ccgtttttgc atattttgga ctgtgtggct
     3781 gaggcgattt cagctcaagg tctgcagaaa gtagcactcc ttggcacaaa atttactatg
     3841 agtgataatt tttacaaaga cggtttattg gagcgtggta tcacatcaat agtaccagat
     3901 gaggaaacgc aacaagaaat tcatcgcatt attttcgagg aactctgttt aggcaagatt
     3961 actgaaagtg ccaaagcgtt ttatcttcag actattcaac aacttagtga acaaggtgca
     4021 gaaggtgtga ttttaggttg taccgaaatc gggttgctga ttaaccaaaa cgacaatccg
     4081 ttaccgtttt tcgatacagc agcattacac agccaaatgg cggtggagtt tattttaggt
     4141 taataaatgt tacaagcggt caaatttgca aaaaatttta ccaattcgac cgcttgcata
     4201 gaatcaaaaa caataggagc aaacaatgtc acaaaaaggc gatatcggcg tgattggact
     4261 cgccgtaatg ggtcaaaatt taattttgaa tatgaatgac aacggcttta aagtggtggc
     4321 gtacaaccgc actacttcaa aagtggatga gttcttagaa ggtgcggcga aaggcacgaa
     4381 cattatcggt gcttactcgt tagaagattt agcggcgaag ttggaaaaac cgcgtaaagt
     4441 gatgctaatg gtgcgtgccg gtgaggtggt ggatcagttt atcgaggctc ttctgccaca
     4501 tttagaagaa ggcgacatca tcattgatgg cggtaactca aactatccgg acaccaaccg
     4561 ccgtgtgaaa gcgttagcgg aaaaaggcat tcgctttatc ggctcaggtg tatcgggcgg
     4621 cgaagagggt gctcgtcacg ggccgtcaat tatgccgggc ggtgatgaat ctgcgtggca
     4681 atatgtgaag ccgattttcc aagcaatttc agccaaaacc gacaaaggtg agccttgttg
     4741 tgactgggtt ggtcgtgacg gagcagggca ttttgtgaaa atggttcaca acggtatcga
     4801 atatggcgat atgcagttaa tttgtgaagc ctaccaattc ttaaaagatg gtttaggttt
     4861 aagctatgat gaaatgcacg aaatctttaa agagtggaaa aacaccgaat tagacagcta
     4921 tttggtggat attaccactg atattcttgc ctacaaagac gaagacggcg agccattggt
     4981 agagaaaatt ctcgacaccg caggtcaaaa aggaacgggt aaatggactg gtattaacgc
     5041 ccttgatttc ggcattccat taacactgat taccgagtcg gtattcgccc gttgcgtatc
     5101 tgcgtttaaa gatcaacgtg ttgcggcttc aagattgttt aacaaagaaa ttggtaaagt
     5161 tgaaggcgac aaaaaagtat gggtagaagc ggtacgccgt gcgttacttg cttccaaaat
     5221 catctcttat gcacaaggct ttatgttgat tcgtgaggct tctgagcaat tcggttggga
     5281 catcaactac ggcaataccg ccctattatg gcgtgaaggt tgtattatcc gcagccgttt
     5341 cttaggcaac attcgtgatg cgtatgaagc caacccatat ttagtgttct taggctcaga
     5401 cccatacttc aaagatattt tggaaaactg cttggcagat tggcgtaaag tagtggcaaa
     5461 atcggtggaa atcggtttac ctgtaccttg tatggcgtca gccattacct tcttagatgg
     5521 ctacacctcg gcacgtttac cggcaaactt actccaagca caacgtgact actttggtgc
     5581 tcatacttat gagcgtacag acaaagctcg cggtgagttc ttccacacca actggacagg
     5641 acgtggcggt aataccgcat cgaccactta tgatgtgtaa ttaatctttt tgagccactt
     5701 aattcgatat taagtggctt ttttattgga tttttcttca aaatatcttg gagtgaatgt
     5761 ttacaagttt gtataataat ggagcgattt ttcatttata tttaaggaga acctatgctt
     5821 accgatcaag atatttttaa tcgctaccaa acaaaaaaat cagaatttga aaaacaggca
     5881 gatatttccc ttattgggca ttccctgttt gatatgtggg gcgatatgga aaacggcacg
     5941 ccgaatttag caggaaaaac agttgccaat ttgggcttat caggcaccag cacccgccaa
     6001 tatttggatg tgattacccg acaagagcga attcaacacg ttggcgataa tgtatttatt
     6061 tttctaggcg ttaatgacat tgtaaaagag ccggattact cgccaaaaca agtgttagag
     6121 tggattggcg aaattgtgga ttctcttcgc aaaattaatc caaatgccaa cttttttcta
     6181 ttggaagcca caccggttaa taatattacc accaccaata atgctgcaat aaaagagctc
     6241 aatgcgtatt ttgaggcaaa tattccatct gatttgactt atgtaaaaac gtgggaatat
     6301 tttgctgaca aagaagggaa tttagatcta actttatgcc acgatggatt acacttcacc
     6361 caagcaggct atgaccgcct agaggcgatt ttaaccaaac atttataatg aaaagcttca
     6421 ataagcaagc ggtctgaatt agcaaaaaat ttccaaatcc tgaccgcttg ttagttaatt
     6481 acagtgcttc aatctcttga tactgcacct gcaatttctc taatccactc aggtactctt
     6541 gcattttttc acgctctttg gcgatgactt gctctggggc tttggcgaca aaggcttcgt
     6601 ttgaaagttt gccttcaatg cgagccactt cacctttaag tttatccatc tctttggtga
     6661 ggcgagctag ctcagcttct ttgttgataa agcctgccat tgggatcaag atctcaccat
     6721 ttgcgaccag tttcgctacc gatagcggtg cattttcacc attttccagc actttcacgc
     6781 tatcaagttt tgccatcgat ttgagcaaca cttcgttagc gttgagaata gagacttcgt
     6841 ccgcagagaa attacgagcg attaaatcta aacctttgct tggtgcgatg ttgctttccg
     6901 cacgaatgtt acgcaccgcc acaatcactt ctttgataaa gttgatttga ctttcggcgg
     6961 tttcatcaat ggcaagcggg tcaaattgtg ggaatttttg caacataatg gtatcggctt
     7021 caatacctac aaagcctttc actttctgcc agatttcttc ggtgatgaac ggcattactg
     7081 ggtggattaa acgcaggagt ttttccaata cattcaccag cgtgcgactt gcgccacggc
     7141 gttgtacatc tgtgcctttg aagaaaatag gtttggtcag ctctaaatac cagtcgcaga
     7201 attgatccca agtaaattcg tagatcgcat ttgccactaa gtcgaaacgg tattggctga
     7261 gtgcggtgcg gaaagtttcc accgtgcggt tgaattggct ttcaatccaa cgatcggcaa
     7321 ggctaaattc cacgtcgcct tcgcttaaat ccaacttgtc attggtcagc acataacggc
     7381 tcgcattcca cagtttgttg cagaagttgc ggtagccttc taaacgtttc atatcccagt
     7441 tgatgtctcg accgttgctg gcaagggcgg tgagggtgaa acgcagagcg tctgtgccgt
     7501 gtgccacaat cccttcggca aattctttgc gggttgcctt agcgattttt tctgccagtt
     7561 gtggctgcat catattacct gtacgttttt cgagcaaatc gtcgagactg atgccgtcaa
     7621 tcatatcaat cgggtctaac acgttccctt tcgatttcga cattttctgc ccttgctcgt
     7681 cacggatcaa gcctgttaca tacacggttt tgaacggcac ttgcggtttg ccgttttcat
     7741 ctttgatgaa gtgcatcgtg aacataatca tacgagccac ccagaagaag atgatgtcaa
     7801 aaccggtaat cagcacatcg gtcgggtgga acattttcag ttctttggtt tgttctggcc
     7861 agcctaacgt tgagaacgtc catagaccag acgagaacca agtatccagt acgtcttcat
     7921 cttgctttaa tggtaagtcc gcaggtaagt tgtgtttcgc acgcacttcc gcttcatctc
     7981 gtgctacata aacattgccc tgctcgtcat accacgctgg aatgcggtgt ccccaccata
     8041 attggcgaga gatacaccag tcttgaatat cacgcatcca agagaaatac aggttttcgt
     8101 actgtttcgg cacgaattgg atttcgccgt cttccaccgc tttggtcgcg acttccgcca
     8161 aaggtttgac agacacatac cattggtcgg tcagcatcgg ctcaatcggc acgccaccac
     8221 ggtcgccata cggcactttt aagtcgtgtg gtttgatttg atctaacagc ccaagagctt
     8281 caaaatctgc cacaattttc ttacgcgcgg caaaacgctc taagccttga tagtcggcag
     8341 gaattggggc ggtgtagtcg gaaagcggtt taccgtctgt gccgataatt tcggcctctg
     8401 cacgaatatc ggcatttaag gtcatcacat tcaccatcgg caagccgtgg cgtttgccca
     8461 cttcgtagtc gttgaaatcg tgggcagggg tgatttttac cacgcccgta ccgaactcac
     8521 gatccacata ctcatcggca ataatcggga tttcacggtt ggcaagcggt aggattacgc
     8581 ttttgccaat cagagcttga taacgctcat cttcagggtg aaccgccacc gcagtatcgc
     8641 ccaacatcgt ttcaggacgg gtcgttgcca ccactaagta atccaagccg tctgcggttt
     8701 ttgcaccatt agccagcgga tagcggaaat gccacagcga gcctttgctt tctttgtttt
     8761 ccacttccaa gtcagaaatc gcagtatgca gtttcggatc ccagtttacg aggcgtttgc
     8821 cacggtaaat caagccttct tcgtgcaagc gaacgaacac ctcttttacc gcattggata
     8881 agccttcgtc catcgtaaaa cgctcacgat cccagtcaat cgagttgcct aagcgacgca
     8941 tttgctggct gatcgtgccg cccgaatagg ctttccaatc ccagattttg ttgataaagg
     9001 cttcacgccc ataatcgtgg cgggttttac cttcttccgc tgcgatttta cgctccacca
     9061 ccatctgagt ggcaatccct gcgtggtcag ttcctgtttg ccaaagggta ttgttgccct
     9121 ccatacggtt aaaacgaatc agcgtatcca tcaaggtttg ctggaaagcg tgccccatat
     9181 gcaaactccc cgtcacattc ggcggcggaa tagcgataca gtagctcggt gcattaatat
     9241 cttcactcgg cttaaaatag ccttgcgatt cccagtgttg gtagagcgct tgctctacag
     9301 cggaagggtt aaaacggtct tccattgtga attgttttgt cattggtttt gttttctctt
     9361 gtttaataat tttttcacta gcttaatctt ataaaaaaga tctttgccgt aaatgctttt
     9421 ataggtaatt agttatttaa attaaggtca actaaaagat taattgggaa ttttttgttc
     9481 ctcttgcatt tttcttcgtt tttcccataa ttgctttcgg ctatttaaac agctttgctc
     9541 aatctcttta atatcatctt ctatattgga tgaaaaattg attaatttga ttgtcggagg
     9601 aaatataccg gttttatctt cttgcgtagt taatgttagt gttcctagat tactgttaat
     9661 ttctttaatt ttaaatgttg atagctcggt atatccccca ctaacaagat aactcaattt
     9721 caactctcta tcgaaaataa gatttaataa tgttggctta gggctttcca atctaaaact
     9781 ccaagttaga ggtattaact cacacagagc tgttgaagtc atttccccta aaatcgaatt
     9841 gtcatcttta ttaaccataa attcaat
//
LOCUS       DANLYI010000080         9840 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.080, whole genome shotgun sequence.
ACCESSION   DANLYI010000080 DANLYI010000000
VERSION     DANLYI010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 9840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9840
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.080"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            complement(<1..407)
                     /locus_tag="PXD81_001396"
     CDS             complement(<1..407)
                     /locus_tag="PXD81_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5537416.1"
                     /translation="MSSIKEIFSTKNSPINVLMQTEWFLGFILIVVYLDAYFQLVHQV
                     PIYIAIQQKEIYLTKTIDYIAAIFFFSVTFTALFTMMRLLHIFILSDLLDKIGCNTYE
                     LPKIEDIHKYEMLSILKIQAAKENNAELRYYVK"
     gene            complement(477..1610)
                     /locus_tag="PXD81_001397"
     CDS             complement(477..1610)
                     /locus_tag="PXD81_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015433643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDL5537417.1"
                     /translation="MKTIKIGLVGTGYIGRCHAIAYAQAPTLFPLKGKIQLEYLAEIN
                     QALADQKAREFGFQRATDDWRKLVTDPNVDVVDICTPNFLHKEIALEAIKHGKHVYSE
                     KPLALTAQDAKEMVIAAQQAGVKTLVGFNYIKNPTTQLARQIIQNGEIGEVVHFYGTH
                     NEDYLANPNTPIDWHCYKAKAGLGTLGDLAAHIVSIAHYLVGSDIERVIGDMQTVIEE
                     RPSPHNPAEKIKVENEDQASSLIRFANGVMGTLESSRISCGRKMGLTYVVTGTKGTLS
                     YTQERMAELKLYLHDDDKSRQGFKTILVGPEHPDYAAFCVSAGHGIGFNDQKAVEIRD
                     LINGIAADDPMYPDFEEGYKISRVLEAIAKSAEEKRWVEVSEI"
     gene            complement(1741..2769)
                     /locus_tag="PXD81_001398"
     CDS             complement(1741..2769)
                     /locus_tag="PXD81_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL5537418.1"
                     /translation="MTTQFLKNMGMVDDKGKIDFIAFFERFGVLIFLILLIIFFTSQN
                     GAFLSQRNIYNVLTEVSIFGIMAVGMTFVILTAGIDLSVGSILAVTAMFAAYIIKGDN
                     TVTLEAGAWGGMSWLIGLSICLGLGTFIGWLHGLGVTKLKLPPFIITLGGMTIWRGLT
                     LVLNNGSPIAGFDEGYRWWGRGDILGVPVPVVIFAVVAIIGYFALHKTRWGRYVYSVG
                     GNPEAARLAGVNINRTLVSVYVVIGALAGLAGFILSARLGSAESVAGVSFELRVIASV
                     VIGGTSLMGGYGRVTGTIIGSIIMGVLINGLVLMDVSAYYQQIIMGLIIILAVAFDTY
                     AKSRRGAL"
     gene            complement(2781..4325)
                     /locus_tag="PXD81_001399"
     CDS             complement(2781..4325)
                     /locus_tag="PXD81_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HDL5537419.1"
                     /translation="MSTPLLEVKDLTKSFSGVMALNGVKLTVGKGEVHALLGENGAGK
                     STLLKALSGAQPQTSGEIIFNGETLSLSDSPFDRQLKGIVTIYQEFNLLPNMTVAENF
                     FLGREPVKGLFVNEKAVNEEAQLVLDNLGLNIKPDTQVSRLSVAQQQMVEIARAMTLN
                     AKLIIMDEPSAALSDKEVETLHQIVRDLKSRGISVIYVTHRLNEVFALCDRFTVFQDG
                     RYSGEGEVKNVNVDQIIKMMVGREVAFTRRPPEETHHTHRPIRLAVKNLHREKPAFDP
                     HGIALHGVSFNIHEGEVLGIAGLVGAGRTEIARCLFGVENYTADEISLDGKPYAATSP
                     LDALEKGVALVPEDRKKEGLVLGLPIKTNMTLPILSKLLKGNLFVDNQKEDDLIETYR
                     QALRIKMASSELEARKLSGGNQQKVILARCMALNPKVLIVDEPTRGIDVGAKSEVHQV
                     LFDMAKQGVAVLVISSDLPEIMAISDRIITLSEGRVTGEIHGDDATEEKLMSMMAVGV
                     QRKAAA"
     gene            complement(4376..5314)
                     /locus_tag="PXD81_001400"
     CDS             complement(4376..5314)
                     /locus_tag="PXD81_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HDL5537420.1"
                     /translation="MKKRIKFVLSALTLGLAMNSFAKDELVVFSLPNLSSPFEVQLQK
                     VAVETSKKLEIKLQVLDGQSSSTKQASDLENAITRGAKGIIISPNDVNAISGAVEEII
                     KEKIPAATLDRKVESSKPVPHFGANNYTGGQEVAKAVKAKYPNGAKIILLTGQPGSTS
                     NIERTKGIRDELAAGGDKYKIVVDQTGNWLRSEGLRIIESVLPTLKEKPEVIISANDD
                     MALGAIEALRSQGLKAGDILVTGFDSTPEALARVKDGWLYLTADQRPSFAVSTALEQV
                     VGNIRESKEVSGADYPPKIILKDNLQEAERFAEISE"
     gene            complement(5646..7157)
                     /locus_tag="PXD81_001401"
     CDS             complement(5646..7157)
                     /locus_tag="PXD81_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HDL5537421.1"
                     /translation="MSQFVQNFINGAVVESQSQRVTSVFNPATGEETKKVKLSLPNEV
                     DAAIAAADQAFKSWSQQSPLRRARILFKFKELLEANFDELARLISSEHGKIYSDAIGE
                     LTRGLEVVEFATGIPHLQKGEFSANAGRGIDIHSIQQPLGVVAGITPFNFPAMVPMWM
                     FPVALACGNTFVLKPSEKDPSVSIRLAELLKEAGLPDGVFNVVQGDKEAVDILLTDSR
                     IQAVSFVGSTAIAQYIYEKGSANGKRVQALGGAKNHALIMPDADVQGTINALLGAAFG
                     AAGERCMALSVAVVVGDELADKIIEELIPKVKALKIGSGLLPQGVLENDMGPVISKEH
                     KAKIESYIDQGVAQGAKLCVDGRGYKVEGFENGYFVGGTLFDNVTPEMTIWQDEIFGP
                     VLSVVRVKDYAEGIALINSHQYGNGSAIFTADGDSARQFTQDVQAGMVGVNIPIPVPM
                     AFHCFGGWKASIFGPLNVYGTDGVRFYTRMKTITTRWPDSSVRSQAAFNFPTL"
     gene            7376..8200
                     /gene="iolB"
                     /locus_tag="PXD81_001402"
     CDS             7376..8200
                     /gene="iolB"
                     /locus_tag="PXD81_001402"
                     /EC_number="5.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-deoxy-glucuronate isomerase"
                     /protein_id="HDL5537422.1"
                     /translation="MSSPLLSKPNFNQHEGLIQHITPENAQWEYVGFDVYQLSKQQIT
                     LESGENELCLVFVTGFGSVYVDDYAFENIGNRTTPFEKIPPYAVYIPPHKSVLVKTEL
                     NLELAVCKAPSQGNYPVRLISPNHIGVEKRGFGNNQRLVHNILPETEAADSLLVVEVF
                     TDEGNTSSYPSHKHDDKHSTEETYLEETYYHRFDPPQGFAMQRVYTDDRTLDECMAVY
                     DKNVVKVPRGYHPVATIAGYNNYYLNVMAGPVRKWKFTWEQDHLWVNSETYKAKFE"
     gene            complement(8276..9727)
                     /locus_tag="PXD81_001403"
     CDS             complement(8276..9727)
                     /locus_tag="PXD81_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HDL5537423.1"
                     /translation="MPNHKFLLLRFQAYINGKFVSSQNGKTFTVQNPTNGEKIGEVAD
                     LGTEETAQAIEAAAVAQKKWQALLPKERSVILRKWFELIMQNQEELAQILSLEQGKPL
                     AESRGEIAYGASFIEWFAEESKRIYGDVLPQDKAGRRLFVTKQPIGVVAAITPWNFPN
                     AMITRKAAPALAAGCAIVIKPAAETPLSALALAQLADQAGIPKGVFNIITSSDVEGVG
                     RELSTNPTVRKLTFTGSTRVGKILMQQSAGTVKKLALELGGNAPAIVFDDADLDTAVE
                     GVFASKFRNGGQTCVCANRIYVQAGIYEQFLAKLSEKVAQIKLGSPFEEEVTIGALIS
                     ERAVEKVESHINDALSLGAELVLGGERHALGGTFFQPTILKNVTQAMKVAREETFAPL
                     APIFKFETEEEVIQWANDTEFGLAAYLFSQNIGRIWRVSEQLEYGIVGINEGIISNEI
                     APFGGWKESGLGREGSKYGIEEFLEIKYLCMKV"
BASE COUNT         2704 a         2307 c         2015 g         2814 t
ORIGIN      
        1 tttttcacat agtatctcaa ctctgcatta ttttccttag ctgcttgtat ttttaatatt
       61 gaaagcattt cgtatttgtg tatatcttca attttaggca gctcataagt gttacatcct
      121 attttatcaa gaaggtctga taaaataaaa atatgtaata aacgcatcat tgtaaataat
      181 gcagtaaatg taactgaaaa aaagaaaatt gctgcaatat aatcaatggt ttttgtgaga
      241 tatatttcct tttgctgtat cgcaatatag ataggcactt gatgaacaag ttggaagtaa
      301 gcatccaaat aaacaactat caatatgaag cctaaaaacc attctgtttg cataagaaca
      361 ttgatagggc tgttttttgt gctaaaaatt tctttaattg aactcataat ttttccttaa
      421 atgtaaaaaa acgtacctca accaatttga gatacgtttc tattttaatg taattattaa
      481 atctcactca cctccaccca ccttttctcc tccgccgatt ttgctatcgc ttccaatact
      541 ctggagattt tatagccttc ttcaaaatcg ggatacatcg ggtcatcagc ggcgatgccg
      601 ttgatgaggt cacggatttc caccgctttt tggtcgttaa aaccgatgcc gtgaccggca
      661 ctcacacaaa aagcagcgta gtcgggatgt tccgggccga ctaaaatggt tttgaatcct
      721 tggcgggatt tatcgtcatc gtgcaaatag agtttgagtt ctgccattcg ttcttgggta
      781 tagctgagcg tgcctttagt acctgtaacg acataggtca gccccatttt acgcccgcag
      841 gaaatgcggg agctttcgag cgtgcccatt accccattag caaatcgtat gagactactt
      901 gcctgatctt cgttttctac tttgattttt tctgctggat tgtgcggact agggcgttct
      961 tcaatcactg tttgcatatc gccgataaca cgctcaatgt ctgaaccaac caaatagtgt
     1021 gcgatactga caatatgtgc ggctaaatct cccaacgtac caagcccggc tttggctttg
     1081 tagcagtgcc aatcaatcgg ggtattaggg ttggcgagat aatcttcgtt atgcgtgccg
     1141 tagaaatgca ccacctcacc aatttcgccg ttttgaataa tttggcgagc cagttgggtg
     1201 gttggatttt tgatatagtt gaaaccgacc aaggttttaa ctcccgcttg ttgagcggca
     1261 atcaccattt ctttggcatc ttgggcggtg agtgcaagcg gtttttctga atagacgtgt
     1321 ttgccgtgtt taatcgcttc taaggcaatt tctttgtgca gaaaatttgg tgtacaaatg
     1381 tccaccacat cgacattcgg atcggtaacc aattttcgcc aatcgtcagt agcacgctga
     1441 aagccaaatt ctcgggcttt ttgatcggct aatgcttggt tgatttcggc aaggtattcc
     1501 agttggattt tgcctttaag tggaaacagg gttggggctt gagcgtaggc aatggcgtgg
     1561 cagcgaccga tatagccggt gccaactaag ccgattttga tggttttcat aatactatcc
     1621 cagtagggtg cgtttgctaa cgcaccatta gaattttcag tgcattggca aacgcaccct
     1681 ataagaaagc ggtctggttt tgcaaaattt ttacaaaatc agaccgcttg tgttagctat
     1741 ctataatgct cctcgtctgc tcttcgcata ggtgtcgaag gcaacggcaa ggatgatgat
     1801 taagcccata atgatttgtt ggtagtaagc ggaaacgtcc attagtacta aaccgttaat
     1861 caatacaccc ataataatgg agccgatgat ggtgcctgtg actcgtccgt atccccccat
     1921 tagggaagtt ccgccgataa ccactgaggc aattacacgt agctcaaaag aaacgccggc
     1981 aacggattcg gcactgccta aacgggcgga gagaatgaaa ccggcaagtc ctgccaatgc
     2041 tccgatcacc acataaacac tgactaaggt acggttaata ttcacaccgg ctaaacgggc
     2101 tgcttccgga ttgccaccta ccgagtacac atagcgtccc cagcgggttt tatgcaatgc
     2161 gaagtaaccg ataatggcga ctacggcaaa aatcactacc ggcaccggta cgcctaaaat
     2221 atcgccacgt ccccaccagc ggtaaccttc gtcaaatccg gcaattggcg agccgttatt
     2281 taacacaagt gttaaaccac gccaaattgt cataccgcca agcgtgataa taaacggtgg
     2341 cagtttgagt ttagtgacgc ctaagccgtg taaccaacca atgaaagtgc ctaaacctaa
     2401 acagatactc aaacctatca gccagctcat tccgccccaa gcaccggctt ctagcgttac
     2461 cgtattatcg cctttgataa tataggccgc gaacattgcg gtaacagcaa ggattgagcc
     2521 gacagagaga tcgatccctg cagttaagat gacgaaagtc attcctaccg ccataatgcc
     2581 gaaaatcgac acttcggtta aaacgttata gatgttgcgt tgcgataaaa atgccccgtt
     2641 ttgtgaagtg aagaaaataa tcagtaagat caagaaaatc agtacgccga aacgttcaaa
     2701 gaaggcgata aaatcgattt ttcctttgtc atcgaccata cccatatttt ttagaaattg
     2761 cgttgtcata caaacctctc ttatgctgcc gctttacgct gaacgccaac agccatcatt
     2821 gacattaatt tttcttcggt ggcatcatcg ccgtgaattt cgccagttac ccgaccttcg
     2881 gaaagagtga taatgcggtc ggaaatcgcc ataatttccg gtaaatcgga ggaaatcact
     2941 aataccgcaa caccttgttt tgccatatca aacagcactt ggtgaacctc agattttgcc
     3001 ccaacatcaa tacctcgtgt cggctcatca acaattagta ctttcgggtt gagtgccata
     3061 caacgggcta aaatcacttt ttgctgatta ccacccgata atttgcgggc ttcaagttcg
     3121 ctacttgcca ttttgatgcg taacgcttgg cggtaggttt cgattaaatc atcttctttt
     3181 tgattatcga caaacaagtt acccttcaat aatttgctta aaatcggcag ggtcatattg
     3241 gttttaatcg gtaaaccgag aactaagccc tcttttttgc gatcttccgg cacaagggca
     3301 accccttttt ccaaagcatc taacggcgag gtggcagcat aaggcttacc gtctaggctg
     3361 atttcatcag ccgtataatt ttctacccca aacaagcaac gggcaatttc agttcgccct
     3421 gcgccaacta agccggcgat ccctaatact tcaccctcgt gaatgttaaa agagacaccg
     3481 tggagagcaa tgccgtgtgg atcgaatgcc ggtttttccc gatgcagatt tttcacagct
     3541 aaacgtattg gtcggtgggt atggtgagtc tcttctggtg gacggcgagt aaaggcaact
     3601 tcacgtccta ccatcatttt aataatttga tcgacattca catttttgac ttcaccctca
     3661 ccagaatagc gcccgtcttg gaaaacggtg aaacggtcgc atagggcaaa cacttcgttt
     3721 aagcggtggg tcacataaat cacgctaatg ccacggcttt ttaaatcacg cacgatttgg
     3781 tgtagtgttt ccacttcttt atcacttaag gcagcggacg gttcgtccat aataatcaac
     3841 ttagcgttta aggtcattgc acgggcaatc tcgaccatct gctgttgtgc cacacttaaa
     3901 cgggaaactt gggtatcagg tttgatattt aagccgagat tatccagcac taattgtgct
     3961 tcctcattca ccgctttttc attgacgaat aaacctttca ccggctctct gcccaaaaag
     4021 aaattttcag caaccgtcat attcggtaac aagttaaatt cttgataaat cgttacgatg
     4081 cctttgagtt ggcgatcaaa cggggaatcg ctcaaactta aggtttcacc gttaaagata
     4141 atttcgccac ttgtttgtgg ctgagcgcct gaaagtgctt ttaataaggt cgattttcct
     4201 gcaccgtttt cacccaacaa tgcgtgaact tcgccttttc ccacagtcag tttcacgcca
     4261 ttgagtgcca taacaccaga gaaacttttt gtcagatctt tcacttcaag taagggagta
     4321 gacatacaac ctccgtaaaa aaacgccctc cggcctcagc cggaaggcag ataaattatt
     4381 cgctgatttc tgcaaaacgt tctgcttctt gcagattatc tttcaggata atttttggcg
     4441 gatagtccgc accgctcact tctttgctct cacggatatt gccaaccact tgctctaatg
     4501 cggtgcttac cgcaaaactt gggcgttggt cagcggtgag atacaaccag ccatctttca
     4561 cacgtgcaag agcctcaggg gttgagtcaa agccggttac taaaatatcg ccggctttca
     4621 aaccttggct acgcaatgct tcaatcgcac caagtgccat atcgtcattc gctgaaataa
     4681 tcacttctgg tttctctttt aaggttggta acacactttc aataatgcgt aatccctcag
     4741 aacggagcca gttaccggtt tgatcaacca cgattttata tttgtcacca ccggcagcta
     4801 attcatcacg aatacctttg gtacgttcaa tgttagaggt tgaacccggc tggccggtta
     4861 aaagaataat ttttgcccca ttcgggtatt tggctttcac tgcttttgcg acttcttgac
     4921 cgcctgtgta gttatttgcc ccaaagtgtg gaaccggctt actgctttca acttttcggt
     4981 ctaatgtcgc agcaggaatt ttttctttga taatttcttc cactgcacct gaaatcgcat
     5041 tcacatcatt cggggaaatg ataatgcctt tagcaccacg ggtaattgca ttttctaagt
     5101 cagaagcctg cttagtagaa gaactttgcc cgtctaatac ttgcaatttg atctccaact
     5161 ttttactggt ttctaccgct actttttgta gttgaacttc aaacgggctg gataggttcg
     5221 gtaagctaaa taccacaagc tcatctttcg caaagctgtt cattgctaag cctaacgtta
     5281 aagcactcaa tacaaattta atacgctttt tcattacaaa atcctctttt taagatggga
     5341 attgacggcg aatattgcca atgttacttc aaactgcgaa ggaaacttaa gtcttatttc
     5401 aataataaac ctcaatgaaa taaatttttc aatttagaat atatcattca aaattttcca
     5461 aaccaaatgc tttgtttcaa atttgttacg tagatcacat ttttaggaag agataaatgc
     5521 aagcggtcaa aacaggtcgc ttttttgcaa aaatttgaat aaaaatgacc gcttgtgaga
     5581 tgaaaaccaa taaaaaagcg gtgatttctc accgctaaac actcaaaaaa gaaatgtact
     5641 cgttattata aggttgggaa attaaatgcc gcttggctac gcaccgagct gtccggccaa
     5701 cgggttgtga tggttttcat acgggtgtag aaacgcacgc cgtctgtgcc gtacacattg
     5761 agaggcccga aaatagaggc tttccaaccg ccaaagcagt ggaacgccat tggcacagga
     5821 attggaatat tcacgcccac cataccggct tgtacatctt gggtgaattg acgtgctgaa
     5881 tcgccatctg cggtgaaaat tgcactacca ttgccgtatt ggtgactgtt aatgagtgcg
     5941 atcccttcgg cataatcttt cacacgcaca acagagagaa ccggtccgaa gatttcatct
     6001 tgccagatgg tcatttcagg tgtcacgtta tcaaacagcg tacctccgac aaagtaaccg
     6061 ttttcaaacc cttcgacttt atagcctcga ccatcgacac agagtttcgc cccctgtgcc
     6121 accccttgat cgatatagct ctcaattttg gctttatgct ctttggaaat caccggcccc
     6181 atatcatttt caagcacgcc ttgtggtagt aagcctgaac cgattttcaa tgctttgacc
     6241 ttaggaatta gctcttcaat gattttatcg gctaattcat caccaacaac taccgctacc
     6301 gatagtgcca tacaacgttc accggctgca ccaaatgctg cgcccagtaa ggcattaatt
     6361 gtgccttgta catcagcatc cggcataatc aaagcgtggt ttttcgcacc gcccaaggct
     6421 tgaacccgtt tgccgtttgc tgagcctttc tcataaatat attgtgcaat tgcagttgag
     6481 ccgacaaaac tcaccgcctg aatgcgagag tcggttaaaa gaatatctac cgcttcttta
     6541 tcaccttgca ccacattgaa tacaccgtcc ggcaagccgg cttcttttaa taattctgcc
     6601 aagcgaatag acacacttgg gtctttttca gacggtttta aaacgaaagt gttaccgcaa
     6661 gcaagtgcca ctgggaacat ccacatcggt accattgccg ggaaattgaa cggggtaatg
     6721 cctgccacta cgcccaacgg ttgctggatt gagtgaatat caatgccacg ccctgcattg
     6781 gcggagaact cacctttttg taagtgtgga ataccagtgg caaattccac cacttctaaa
     6841 ccacgggtta attcgccaat cgcatcggaa tagattttgc cgtgttctga gctgattaaa
     6901 cgagccaact catcaaagtt tgcttccaat aactctttga atttaaataa aattctggca
     6961 cggcgtagtg gtgattgttg tgaccagctt ttgaaagcct gatcggccgc tgcaattgcc
     7021 gcatcgactt cgtttggcag gcttaatttg acttttttgg tctcttcacc ggttgcggga
     7081 ttaaataccg aggtaacacg ctggctttgg ctttcaacga ctgcaccgtt aataaaattt
     7141 tggacaaatt ggctcattgg aggctcctta tcaactgatt gagaggaaaa acggataaaa
     7201 tacagccgat aaactaccgc taaaaatgga aaatatcaat acataatact aaaaaatgaa
     7261 aattttattt catttttgtg atctaagtag caagtttaca gattgccggt attaaaatga
     7321 gacaaaattt cgtatagttt gaaagcgtat tccatttcgt taagcgaggt ttcctatgtc
     7381 tagtccatta ttgtctaaac ccaatttcaa ccaacatgaa gggttaattc agcacattac
     7441 ccctgaaaat gcacagtggg aatatgttgg atttgatgtt tatcagctca gtaagcaaca
     7501 aattacgctg gaaagtggtg aaaatgaact ttgtctagtc tttgtgaccg gttttggctc
     7561 tgtctatgtt gatgattatg cttttgaaaa tatcggcaac cgtaccacgc catttgaaaa
     7621 aattccgcct tatgcggtct atatcccgcc acacaaaagc gtgttggtta aaaccgaact
     7681 caatttagaa ttggcagtgt gcaaagcacc aagtcagggt aattatccgg tgcgtttgat
     7741 tagcccgaac catatcggtg tggaaaaacg gggctttggc aacaatcaac gcttggtgca
     7801 taatattctg ccggaaaccg aagcagccga cagtttgctg gtggtggaag tctttaccga
     7861 tgaaggcaat accagttcct acccaagcca caagcacgat gacaaacact ctaccgaaga
     7921 aacctatttg gaagagacct actaccaccg cttcgatcca ccacaaggtt ttgcaatgca
     7981 acgggtttat accgatgatc gcacacttga tgaatgtatg gcggtgtatg ataaaaatgt
     8041 ggttaaagtg cctcgtggct accaccctgt tgctaccatt gccggctata acaactacta
     8101 cctaaatgtt atggcagggc cggtacgcaa atggaaattt acttgggaac aagatcatct
     8161 ttgggtgaac agtgaaacct ataaagccaa atttgagtaa tgcaagcggt ctttttccac
     8221 caatttttta caaaataagg gattaaaaat gggacgcttt tggcgtccca ttcggttata
     8281 ctttcataca aagatatttt atttctaaaa actcttcaat accgtacttc gaaccttccc
     8341 gcccaagtcc agactctttc cagccgccga acggagcgat ttcgttactg atgatcccct
     8401 cattaatccc gacaatgcca tattccaact gctccgatac tcgccaaatt cgcccgatat
     8461 tttggctgaa taagtaagcc gccaaaccaa actcagtatc attcgcccac tggataacct
     8521 cttcttcggt ttcaaatttg aaaatcgggg caaggggagc aaaggtttcc tcacgagcca
     8581 ctttcatcgc ttgagtgaca tttttcagaa tggttggctg gaagaatgtg ccacccagtg
     8641 cgtggcgttc accgcctaac accagttctg ccccaagtga aagggcatcg ttaatgtggc
     8701 tttcgacttt ttccaccgct cgttcactaa ttagtgcacc tatcgtgacc tcttcctcaa
     8761 atggcgagcc aagcttgatt tgagccactt tctcactcaa ttttgcaaga aattgctcat
     8821 aaatgcccgc ttgcacataa attcggttag cacagacgca ggtttgccca ccgttacgga
     8881 atttagaggc aaacacgccc tcaaccgctg tatctagatc cgcatcatca aacacaatcg
     8941 ccggtgcatt accgcctagc tccaatgcca atttttttac cgtccctgcc gattgttgca
     9001 ttaaaatttt gccgacacgg gtggagccgg taaaggtgag tttgcgtacg gtcggattag
     9061 tgctcaactc ccgccctaca ccttcgacat cgctactggt aataatgtta aatacccctt
     9121 tgggaatacc tgcttgatcc gctaattggg ctaatgctaa ggcagaaagc ggggtttctg
     9181 ccgccggttt aatcacaatc gcacaacctg ccgctaacgc cggtgccgct ttacgggtaa
     9241 tcattgcatt tgggaaattc cacggtgtaa ttgctgccac cacgccaatg ggttgtttag
     9301 tcacaaacaa gcggcgaccg gctttatctt gtggcaagac atcgccataa atccgcttgc
     9361 tttcttcggc aaaccactcg ataaaactgg ctccataggc aatctcgcct cgactttctg
     9421 ccaatggttt accctgttct agacttaaaa tctgggcgag ttcttcttgg ttttgcataa
     9481 tcagctcaaa ccatttacgc aaaatcacgg aacgctcttt gggcaataat gcctgccatt
     9541 ttttctgggc aactgcggct gcttcaatcg cttgagcggt ttcttccgtg cctaaatcag
     9601 ccacctcacc gattttctca ccattagtcg gattctgtac cgtaaaggtt ttcccatttt
     9661 ggcttgaaac aaatttaccg ttaatatagg cttggaaacg gagtagcaaa aatttgtgat
     9721 ttggcataat cactccttga atggttaaaa agagaacaat attgactata atccaatttc
     9781 agtaaagaga aaagagctga aaaataactt tttactcaat aataaagcac cttagacaag
//
LOCUS       DANLYI010000081         9835 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.081, whole genome shotgun sequence.
ACCESSION   DANLYI010000081 DANLYI010000000
VERSION     DANLYI010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 9835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9835
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.081"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            87..350
                     /locus_tag="PXD81_001404"
     CDS             87..350
                     /locus_tag="PXD81_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDL5537424.1"
                     /translation="MSTLEDIKKTAKDWHRADIIAALHKAGWSLRQLSLKHGYSQSST
                     LKNAVDRPWLKGERIIAEAIGISPEEIWPERYAQRNHKKFADR"
     gene            366..896
                     /locus_tag="PXD81_001405"
     CDS             366..896
                     /locus_tag="PXD81_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL5537425.1"
                     /translation="MSENSLKTHYSAKELAELSLACLPKTKQGVLYQAKKQGWTTHKK
                     VGVGGEILEFALSNLPQEIQTEIRSRFMSAVVEAKPKKLPAVRAEVDVGNLTTKQREI
                     ADARMALVAYVLELEGSMSRIKAITYLCNLAKQGEMPPHLVELVAVANAKKTAKRTIV
                     PIFSPIMPDFPLDSRD"
     gene            complement(912..2039)
                     /locus_tag="PXD81_001406"
     CDS             complement(912..2039)
                     /locus_tag="PXD81_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005624872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL5537426.1"
                     /translation="MNKLKSINLQVYIMLIAIVVIMSFFSIATDGAYLSARNISNLLR
                     QTSITGILAIGMVFVIISAEIDLSVGSLMGLLGGFAAISNVWWGWPLSLTIIVTLILG
                     VMLGAWSGWWVAYQKVPSFIVTLAGMLAFRGVLVGLTNGTTVSPISNEMTTIGQGYLP
                     DMVGMILGTIGIFCFIVWGSYQRKARQKLNLTVPSMSKDTLKYGLVAIIVLGAIYLLN
                     DYRGVPFPVLVLVILAIAGTFLARKTAFGRHIYAIGGNIDAARLSGISVEKVKLAIFA
                     LNGLLVAIAGLILSSRLGAGAPSAGQNAELDAIAACVIGGASLAGGIGSIYGVVIGAF
                     IIALLDNGMSMLDVPTFWQYIVKGGILLLAVWVDTISKKKA"
     gene            complement(2043..3557)
                     /gene="xylG"
                     /locus_tag="PXD81_001407"
     CDS             complement(2043..3557)
                     /gene="xylG"
                     /locus_tag="PXD81_001407"
                     /EC_number="7.5.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011608742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter ATP-binding protein"
                     /protein_id="HDL5537427.1"
                     /translation="MAKLLEMKNITKKFGEVVALNNISISLETGEILSLCGENGSGKS
                     TLMKVLCGIYPAGDYEGEIYFSGEKLIARNIKDTEEKGISIIHQELTLVKNMSILENM
                     FLGNEITKSGITDDNEMYLRCKTLLEQVQLDIDPNTKVGELGLGQQQLVEIAKALNKQ
                     VRLLILDEPTASLTEKETEILLSLIKDLQAHNIACVYISHKLNEVKAISDKICVIRDG
                     EHIGTRSAEGMSEDDIITMMVGREITSLYPHEPHNIGEEVLRVENLTAWHPTNTHIKR
                     VDNANFVLRQGEILGIAGLVGSGRTEMAQCIFGSYQGKYQGDIFLHKQPIKIKNCAQA
                     IENHIVMVPEDRKKHGIIPIMGVGKNITLSSLPQFCFGKKVINEPLEETIINQSIAKL
                     KVKTSSPELAIGRLSGGNQQKAILAKCLLLNPKILILDEPTRGIDVGAKYEIYKLINQ
                     LAKEGMAIIVISSELPEVLGISDRVLVMHQGKIKADLINQDLTQEKVMEAALKE"
     gene            complement(3624..4625)
                     /gene="xylF"
                     /locus_tag="PXD81_001408"
     CDS             complement(3624..4625)
                     /gene="xylF"
                     /locus_tag="PXD81_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005576719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter substrate-binding
                     protein"
                     /protein_id="HDL5537428.1"
                     /translation="MKLKSKLLAVATATLMVLSNASVAKDLKIGMSIDDLRLERWQKD
                     RDIFVNKAESLGAKVFVQSANGDATAQISQIENMLNKDIDVLVIIPFNGEVLSNVIAE
                     AKKEGVKVLAYDRLINNADVDFYVSFDNEKVGELQAKSIIGQKPEGNYFLMGGSPVDN
                     NAKLFRKGQMKVLQPLIDSGKIKVVGDQWVDSWLAEKALQIMENALTANKNNIDAVVA
                     SNDATAGGAIQALSAQGLSSKVAISGQDADLAAIKRIIAGTQTMTVYKPITNLADKAA
                     EIAVALGKDEKVEMNAQLNNGMKEVPAYLLEPVVVTKENIDDTVIKDGFHTKDAVYK"
     gene            4867..6186
                     /gene="xylA"
                     /locus_tag="PXD81_001409"
     CDS             4867..6186
                     /gene="xylA"
                     /locus_tag="PXD81_001409"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012072255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HDL5537429.1"
                     /translation="MSNYFDKISKVQYEGANSTNPFAFKHYNPDEVVLGKTMAEHLRL
                     AVCYWHTFCWTGNDMFGVGSLDRSWQKTGDLLQGAKQKAEIAFEFFQKLGIPYYCFHD
                     VDVAPEGNNFKEYQHNFNTIVDILEQKQAETGVKLLWGTANCFTNPRYMSGASTNPNP
                     EVFAWAAAQVFTAMNATKRLGGENYVLWGGREGYETLLNTDLKREREQIGRFMQLVVE
                     HKHKIGFNGTLLIEPKPQEPTKHQYDYDVATVYGFLKQFGLEKEIKVNIEANHATLAG
                     HTFQHEIATATALDIFGSIDANRGDPQLGWDTDQFPNSVEENTLVMYEILKAGGFTTG
                     GFNFDAKIRRQSTDPYDLFHAHIGAIDVLALSLKRAAKMLEDQALQKVVDNRYAGWNQ
                     DLGLQILNGKASLEDLAKIVETQGLDPKPVSGQQEYLENLVNSYIYR"
     gene            6339..7784
                     /gene="xylB"
                     /locus_tag="PXD81_001410"
     CDS             6339..7784
                     /gene="xylB"
                     /locus_tag="PXD81_001410"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014992297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HDL5537430.1"
                     /translation="MYIGIDLGTSGVKVVLLDENQHIIAITQKSLPIFRPHPLWSEQN
                     PQDWWNATNEAMLELSATQDLQAVKAIGLTGQMHGATLLDNADNVLSPAILWNDGRSF
                     MECEELEQLVPNSREITGNLMMPGFTAPKLRWVDKHQPDIAEKVSKVLLPKDYLRLLM
                     SGEYASDMSDASGTMWLDVGKRNWNKSLLNACGLDIENMPKLFEGNQITGYLRPTLAE
                     QWKMKSVPIVAGGGDNAAGAIGIGLYQTGQAMLSLGTSGVYFVVSDKFQANPQKAVHS
                     FCHALPDRWHLMSVILSAASAVDWAVKSLGLKDVSTLFQQVEASQTASDAIFLPYLSG
                     ERTPHNDPYAKGVFWGLSHNDNQITMAKAVIEGVSFALAEGIEVLHETGVVADNIALI
                     GGGAKSVYWRQLLADISGKTFEYRTSGDVGPALGAAKLAQIALNPNQDIATFCQPLPL
                     EQVYQPTPARYAEYQQKRQKFTELYRRLKGL"
     gene            complement(7847..8170)
                     /locus_tag="PXD81_001411"
     CDS             complement(7847..8170)
                     /locus_tag="PXD81_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QacE family quaternary ammonium compound efflux
                     SMR transporter"
                     /protein_id="HDL5537431.1"
                     /translation="MNVWILLAISICLEIAATNLLKLSNGFTKAIPTIGSLALYGLSF
                     YFLSIIFRTLPVGIVYAIWSGVGIVLTALVAYFAFGQKIDLAGLIGISLIIAGVLVIN
                     LFSKV"
     gene            8294..8836
                     /locus_tag="PXD81_001412"
     CDS             8294..8836
                     /locus_tag="PXD81_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase family protein"
                     /protein_id="HDL5537432.1"
                     /translation="MAQVYIVHGYTANADKHWFPWLEQELAKCGVSCERLNMPDSAKP
                     SLDGWLNHLAQNVELTDQTVFVGHSLGCIAILNFLAKNYAKIKGAVFVSGFYQPVENL
                     PELSAFTNYYAILPSVSSFPSYVVSALDDKVVNHQYSDALAQHLHADYIRLNQGGHFL
                     DREGVTELPVVLELVKKLLE"
     gene            complement(9007..9831)
                     /gene="dapF"
                     /locus_tag="PXD81_001413"
     CDS             complement(9007..9831)
                     /gene="dapF"
                     /locus_tag="PXD81_001413"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012263271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HDL5537433.1"
                     /translation="MQFSKMHGLGNDFMVVDGVTQNVYLTEEMIRTLSDRHRGIGFDQ
                     LLLVEPPYDPELDFHYRIFNADGSEVSQCGNGARCFARFVTLKGLTNKKDIHVSTAKG
                     KMVLSLQEDGKVRVNMGEPIWEPSQVPFTANKFEKNYILRTELQTVLCGVVSMGNPHC
                     VLQVDDVKTAPVNELGPLLESHERFPERANISFMQVVNRNHVKLRVYERGAGETQACG
                     SGACGTVAVGIMQGLLDNKVQVDLPGGTLIIEWQGVGHPLYMTGDATHIYDGFIKL"
BASE COUNT         2920 a         2105 c         2020 g         2790 t
ORIGIN      
        1 aatcaactat aaagcaacct ttttacactt tatttttagt aaaaataggt atattatcta
       61 aatcgttaag cgaaaggagt tatctaatga gtacattgga agatattaaa aaaacagcga
      121 aagactggca tagggccgac atcatcgctg ctttacataa agcgggttgg tcgcttagac
      181 aactctcatt gaagcatggc tacagtcaat ccagcacctt gaaaaacgct gtagatcgtc
      241 cgtggttaaa gggtgagcgt attattgccg aagcaatcgg tatatcaccc gaagaaattt
      301 ggcctgaacg ttatgctcaa cgaaaccata aaaaatttgc cgacagatag cttaaagagg
      361 tttttatgag tgaaaacagt ctaaaaacac actattcggc aaaagaattg gctgagttaa
      421 gtttagcctg tttacctaaa acaaagcaag gagttttgta tcaggcaaag aaacaaggat
      481 ggactactca taaaaaggtt ggagttgggg gagaaatttt agaatttgca ctctcaaacc
      541 tccctcaaga aatccaaacc gaaatccgct cccgctttat gtcggcggtg gtggaagcca
      601 agccgaaaaa gttaccggca gtgcgtgcgg aggtggatgt gggaaacctg accaccaaac
      661 agcgagaaat tgctgatgca cgaatggcac tggtggcgta tgtgttggaa ctggaaggct
      721 caatgagccg gattaaagcg ataacctacc tttgcaacct tgccaaacaa ggcgaaatgc
      781 caccgcactt ggtggagctg gttgccgtag caaatgctaa aaagacggca aaacgcacaa
      841 tagttcccat ttttagcccg attatgccgg atttcccgtt ggatagtcga gattgaccgc
      901 ttgttatcca attaggcttt tttcttgctg atggtatcta cccaaactgc aagcagtaga
      961 attccacctt taacgatata ttgccagaag gttggcacat caagcatact cataccgtta
     1021 tccagcaaag caataataaa tgctccaata actacgccat aaatgctacc gataccgccg
     1081 gctaaactgg ctccaccaat cacgcaggcg gcaatcgcat ctaattcggc attttgcccg
     1141 gcggaaggtg cgccggctcc aagacgtgag cttaaaatca agcctgcaat ggcaactaat
     1201 aagccgttta aggcaaaaat cgcaagtttg actttttcaa ctgaaatccc cgagaggcga
     1261 gcggcatcaa tgttgccacc aatcgcataa atgtgacgcc cgaaggcggt ttttctggcc
     1321 aagaatgtgc cggcaatggc tagaatcacc aacaccaata ccgggaatgg cacaccacgg
     1381 taatcattga gtaggtagat cgcacctaat acgataattg caactagtcc gtatttcagc
     1441 gtgtctttcg acatagaagg cacagtaaga ttgagttttt gtctggcttt gcgttggtag
     1501 ctaccccaaa caataaagca aaatatcccg atagtaccta aaatcatacc caccatatca
     1561 ggcaaatagc cttgaccaat ggttgtcatt tcattgctaa tcggtgaaac ggttgtcccg
     1621 ttggttaagc cgaccaatac accacgaaac gccagcatac cggcaagagt tacaataaag
     1681 gacggcactt tttgataagc aacccaccaa ccgctccaag cacccagcat gacgcctaaa
     1741 attaaagtaa ctataatagt taacgagagc ggccagcccc accatacatt actgatggcg
     1801 gcaaatccac cgagtaagcc cattagcgag ccaaccgata aatcaatttc tgcggaaata
     1861 atcacaaata ccataccgat ggcaagaata ccggtaatgg aggtttggcg taacaggttg
     1921 gagatatttc tggcacttaa ataagcaccg tcagttgcaa tagagaaaaa gctcataatc
     1981 acaacaattg caatcagcat aatataaact tgtaaattaa tcgattttaa cttattcata
     2041 ggttactcct tgagtgccgc ttccattact ttttcttggg taaggtcttg gttaatcaaa
     2101 tctgctttaa ttttgccttg gtgcataacc aatacacgat cactaatgcc taaaacttca
     2161 ggtaattcgg aggaaatgac gataattgcc ataccttctt tagctaattg gttgatgagc
     2221 ttataaattt catattttgc tcccacgtcg ataccacggg tcggctcatc taaaatcagg
     2281 attttagggt taagcaatag gcattttgcc aaaattgctt tttgctgatt accgccactt
     2341 aaccgtccaa ttgctaattc aggcgaggaa gttttcactt tcagttttgc aatagattgg
     2401 ttgataatgg tttcttccag cggttcattt attacttttt tgccaaaaca aaattgcggt
     2461 aatgaagaga gagttatgtt tttacccacc cccataattg gaatgatgcc gtgttttttg
     2521 cgatcttccg gcaccatcac aatatggttt tcaatcgctt gtgcacaatt tttgatttta
     2581 atcggttgct tatgcagaaa aatatcacct tgatatttgc cttggtaaga gccaaaaata
     2641 cattgtgcca tctcggttcg ccctgaaccg accaaaccag caatacctaa aatttcacct
     2701 tggcgtagta caaaattagc attatcaacc cgtttgatat gtgtattggt tgggtgccaa
     2761 gcggttaaat tttcaactcg aaggacttct tcaccaatat tgtgtggctc gtgtgggtag
     2821 agcgaggtaa tctcacgtcc aaccatcatc gtaataatat cgtcttcact cattccctct
     2881 gcactgcgag taccgatatg ttcgccgtct cgaatgacac agattttgtc ggaaatggct
     2941 ttcacttcat tcaatttgtg tgaaatataa acacaagcaa tattgtgagc ttgtaaatct
     3001 ttaattaaac tcagcagaat ctcggtttct ttttcggtta acgaggcggt aggctcgtct
     3061 aaaatcaaca accgtacctg tttattcaat gctttggcaa tttcaaccaa ctgttgttgc
     3121 cctaaaccta gctctccaac tttggtgttt gggtcaatat ccaactgcac ttgttcaagt
     3181 aaggttttac aacgcagata catttcattg tcatctgtaa tccctgattt ggtaatttca
     3241 ttccctaaaa acatattctc caaaatcgac atatttttta ctaaggtcaa ttcttggtga
     3301 ataatcgaaa tgcctttttc ttcagtatct ttgatatttc tggcaatcaa tttttcgccg
     3361 gaaaaataga tttccccttc ataatcaccg gcaggataaa ttccacataa tactttcatc
     3421 agagttgatt tgcctgaacc attttcacca cataaagaaa gtatttcgcc ggtttcaagg
     3481 gaaattgaaa tattatttaa tgccacaacc tcaccgaatt ttttagtgat gttcttcatt
     3541 tctaataatt tcgccatact tagttagccc cttattttca aaaatggcga ggaagcctcg
     3601 ccatctagaa aaattaaagt gatttattta tatactgcat ctttggtatg gaagccgtct
     3661 ttaattactg tgtcatcaat attttcttta gttacaacaa ccggctctaa taaatatgcc
     3721 ggtacttctt tcataccgtt atttaattga gcattcattt ctactttctc atctttacct
     3781 aatgcaacag caatttcggc agctttgtcg gctaaattgg taattggttt atacactgtc
     3841 atcgtttgcg taccggcaat aatacgttta attgcggcta aatctgcatc ttgtccggaa
     3901 attgcaacct tgctggataa cccttgggca cttaacgctt gaattgcacc gcctgcggtt
     3961 gcatcgtttg aagctacaac tgcatcaata ttatttttat tagcagttag tgcattttcc
     4021 ataatctgta atgctttttc agcaagccaa gaatctaccc attgatcgcc taccacttta
     4081 atttttccac tgtcaattaa cggttgtagc accttcattt gacctttacg gaacagcttc
     4141 gcgttattat ctacaggtga accacccatt aggaaataat tcccctccgg cttttgccca
     4201 ataatgcttt tagcctgcaa ttcacctact ttttcattat caaatgaaac ataaaaatcc
     4261 acatcggcat tattgattaa gcggtcgtaa gcaagtacct taacaccttc tttcttcgcc
     4321 tcggcaatca cattggataa cacttcgccg ttaaacggaa taatcaccaa tacatcaata
     4381 tctttattca acatattctc aatttgtgag atttgtgcgg ttgcatcgcc atttgccgat
     4441 tgaacgaaga cttttgctcc taagctttcc gctttattca caaaaatatc tcggtctttt
     4501 tgccaacgct ctaagcgtaa atcatcaatt gacataccga tttttagatc tttagcaaca
     4561 gaagcattgc tgagtaccat taaagtcgcc gtagcaacgg ctaataattt agacttaagt
     4621 ttcatagttc acctcattgg ttatgaaaat gtgagcatct ttttcgcccg taagcagtct
     4681 gggcttattt caaatgaagt tcaattatca tatttattaa tatcattatg atttttcact
     4741 tttgtgatct acgccacatt aaccaataat cgaaatccaa aaacgaaata tcgtaattga
     4801 aggttttgca taattttccg atgatgttac cgaaagatta tttttccaat tcataggagc
     4861 attattatgt ctaactattt cgacaaaatc tcaaaagtac aatatgaagg ggctaattcc
     4921 accaacccgt ttgcattcaa acattacaac ccggatgaag tggttttagg caaaacgatg
     4981 gcagaacatc tgcggttagc ggtttgctat tggcacacct tctgctggac gggtaacgat
     5041 atgtttggcg tgggttcgct tgacagaagc tggcaaaaaa cgggggattt actgcaaggt
     5101 gcgaagcaaa aagcggaaat tgcctttgaa tttttccaaa aattaggcat tccttactac
     5161 tgcttccacg atgtggatgt tgcaccggaa ggcaacaatt tcaaagaata tcagcacaat
     5221 ttcaacacta ttgtggatat tttagagcaa aaacaagctg aaacaggcgt gaaattatta
     5281 tggggtacgg cgaactgctt taccaatcct cgctatatgt cgggtgcttc aaccaatccg
     5341 aatccggaag tctttgcttg ggcggcagca caagtcttta ccgcaatgaa cgcaaccaaa
     5401 cgcttaggcg gcgaaaatta tgtattatgg ggcggacgtg aaggttatga aaccctactc
     5461 aacactgact taaaacgtga gcgtgaacaa atcggtcgct ttatgcagtt agtggtggaa
     5521 cataaacaca aaatcggttt taacggcact ttattaattg agccaaaacc acaagaacca
     5581 accaaacatc aatatgacta cgatgttgcc accgtttacg gtttcttaaa acagtttggt
     5641 ctagaaaaag agatcaaagt caacattgaa gcaaaccacg ccactcttgc agggcatact
     5701 ttccaacacg aaattgcgac tgccaccgca ttagatattt ttggctcgat tgatgccaac
     5761 cgaggtgatc cgcaattagg ttgggatacc gaccaattcc caaacagcgt ggaagaaaac
     5821 acccttgtga tgtatgaaat cttaaaagca ggtggtttca caacgggcgg tttcaacttt
     5881 gatgcgaaaa tccgccgcca aagtaccgat ccgtatgatt tattccacgc acatatcggg
     5941 gcaattgatg tgttagccct ttcgctaaaa cgtgcggcaa aaatgctgga agatcaagca
     6001 ttacagaaag tagtagataa ccgctacgct ggctggaacc aagatttggg cttacaaatt
     6061 ctaaacggta aagcaagcct tgaagatttg gcaaaaatag tggaaaccca agggttagat
     6121 ccaaaacctg tttccggtca gcaagaatat ttggaaaatt tggtaaatag ctatatttac
     6181 cgttaatcta caagcggttg ttttttatca attttttgca aagttaagct cgatctgatc
     6241 cccctctttg ctaaagaggg gtgatagatc tccaaaactt aaaattgcaa atgttccgaa
     6301 taaaatgacc gcttgtattc aataacaaag gaatatttat gtacattgga atcgatctcg
     6361 gcacatcggg tgtgaaagtc gtgttactcg atgaaaatca acacattatt gccatcacac
     6421 aaaaatcact tcctattttt cgtccacacc cattgtggtc tgaacaaaat ccgcaagatt
     6481 ggtggaatgc caccaatgaa gcgatgttag aactttccgc tactcaagat ttacaagcag
     6541 taaaagcgat tggtttaacc gggcaaatgc acggagcgac tttactagat aacgcagata
     6601 atgtactttc ccctgcgatt ttatggaatg acggacgtag ttttatggaa tgcgaagagt
     6661 tagaacagct tgtgccaaat agccgtgaaa ttacaggcaa cctgatgatg ccgggcttta
     6721 ccgctccgaa attgcgttgg gttgataaac accagcccga tattgccgaa aaagtcagca
     6781 aagtcttgct accgaaagat tatctccgct tgttgatgag cggcgaatat gcctctgata
     6841 tgtctgatgc ctccggcaca atgtggcttg atgtgggtaa acggaattgg aacaaatcgc
     6901 tactaaatgc ttgcggatta gacattgaaa atatgccgaa actgtttgaa ggcaatcaaa
     6961 tcacaggtta tttacgtcca acccttgccg agcaatggaa aatgaaatct gtgccgattg
     7021 tggcaggcgg tggcgataat gctgccggtg caatcggcat tggcttatac caaaccgggc
     7081 aagctatgct ctcattgggg acttcagggg tttattttgt agtgagcgat aaatttcagg
     7141 ctaatccgca aaaagcagtg catagttttt gccacgcttt acctgatcgt tggcatctga
     7201 tgtcagtaat attaagtgcc gcttctgcgg tagattgggc agtaaaatca ttgggactaa
     7261 aagatgtatc gactttattc caacaggttg aagcaagcca aacggcatct gatgctattt
     7321 tcttacctta tttatcgggt gaacgtacac cgcataatga cccttatgcg aaaggcgtat
     7381 tttggggatt aagccataat gacaaccaaa taacgatggc aaaagccgtg attgaagggg
     7441 taagtttcgc tttggctgaa gggattgagg tactccacga aactggtgta gttgctgaca
     7501 atattgcatt aattggcggt ggagcaaaaa gtgtgtactg gcgacaacta cttgccgata
     7561 ttagcggtaa aacctttgaa taccgcacca gtggtgatgt cggtccggca ttaggggcgg
     7621 caaaactcgc tcaaattgcg ctaaacccaa atcaggatat tgctacgttc tgtcagccgt
     7681 tacccctgga acaggtttac caaccaaccc ctgcccgcta tgcagaatat caacaaaaac
     7741 gccaaaaatt tactgagctt taccggcggt taaaggggtt ataattcttg ccaactatta
     7801 caagcggttg attttgcaga tttttttgta aaatcaaccg cttgtatcac actttagaaa
     7861 acaaattaat caccaataca ccggcaataa tgaggctgat gccgattaag cctgccaagt
     7921 caattttctg tccgaaagca aaataagcaa caagggcggt aaggacaatc cctacgccgg
     7981 accaaatagc atacacgatt ccgaccggta gggtgcggaa aataatagag agaaagtaaa
     8041 aggataaacc gtataaggcg agcgagccaa tagtcggtat agctttggtg aagccgttgc
     8101 ttaatttgag taaattggtg gcagcgattt cgaggcagat ggaaatcgca agtaaaatcc
     8161 aaacgttcat tgatgacctc tttgcgtaaa tgcgtgctat tttacctgag ttgcaaaaaa
     8221 atttaaaaaa atcaccgctt gttttttcct gattaagcta tggtacaatc tttggtgaat
     8281 caggaggtgt gaaatggcac aggtttatat cgtacacggt tatacggcga atgccgataa
     8341 gcattggttt ccttggctgg agcaagaatt agcgaagtgt ggtgtcagtt gtgagcggtt
     8401 gaatatgccg gattcggcaa agccgtcatt agatggttgg ttaaatcatc ttgcacaaaa
     8461 tgtggagctg accgatcaaa cggtttttgt cgggcatagt ttaggttgca ttgccatttt
     8521 gaatttttta gccaaaaatt acgccaagat taaaggggcg gtgtttgtgt cggggttcta
     8581 tcaaccggta gaaaacttac ccgaactgtc tgcctttacc aactattatg cgattttgcc
     8641 gagcgtatcg agctttccaa gctatgtggt aagtgcgtta gatgataagg tcgtgaatca
     8701 ccaatacagt gatgcgttag cccaacattt gcacgcggat tatattcggt taaatcaagg
     8761 cgggcatttt ttggatagag aaggtgttac cgaattacct gtggtgttgg agttggtgaa
     8821 gaagttactc gagtgaaacc tgttttggta caagcgagga cgcttgcacc atctataagt
     8881 tgaatttaaa caaaagttat acatcagtct gatagtgcca gcgtccacgc tggtactttt
     8941 gtataaacca accctatttt gcaaaaaaac ggtaaaaata aaccgcttgc ctccctatgg
     9001 gaaaacttac agtttaataa atccatcata aatgtgggtg gcatcgccgg tcatatacag
     9061 aggatgccct actccttgcc attcaataat cagcgttcca ccgggtaaat ccacctgcac
     9121 tttgttatct aacaagcctt gcataatgcc gaccgccact gtgccgcacg caccgctacc
     9181 acaggcttgg gtttcgcctg ctccacgctc atacacccgt aatttaacgt gattgcgatt
     9241 cactacttgc ataaagctga tattcgcccg ttcaggaaaa cgctcgtggc tttctagtaa
     9301 cggcccgagt tcatttaccg gggcggtttt cacatcatca acttgcagta cgcagtgcgg
     9361 attccccatt gaaaccacgc cacataacac ggtttgtagc tcggtgcgta aaatatagtt
     9421 tttctcaaat ttatttgctg taaaaggcac ttgcgaaggt tcccaaatcg gttcgcccat
     9481 attcacacgc actttgccgt cttcttgcaa acttagcacc attttgcctt ttgcggtgct
     9541 aacgtgaatg tcttttttgt tggttaagcc tttgagcgta acaaaacggg caaaacatct
     9601 cgccccattg ccacattgcg acacttcact gccgtctgca ttaaaaatgc gatagtgaaa
     9661 atctaattca gggtcgtaag gcggttcaac caataaaagt tggtcgaaac cgataccacg
     9721 atggcgatca gaaagcgtac ggatcatctc ttcggtaagg taaacgtttt gggttacacc
     9781 atcaaccacc ataaaatcat tgcctaagcc gtgcattttt gaaaattgca tatta
//
LOCUS       DANLYI010000082         9710 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.082, whole genome shotgun sequence.
ACCESSION   DANLYI010000082 DANLYI010000000
VERSION     DANLYI010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 9710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9710
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.082"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            379..1383
                     /locus_tag="PXD81_001414"
     CDS             379..1383
                     /locus_tag="PXD81_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDL5537434.1"
                     /translation="MPGDANKIISNGTSAGGALSTLLGASADHYDYEPYLKEAGALNA
                     SDKIFAVSAYCPITNLENADMAYEWQFNGVNEYSRIDMSRLNAAEFNDRSKPKPKPKI
                     EGSLNEAEIKVSNELAERFPTYLNSLHLVDEKGNPLTLDPKGNGSFKDYLSEVVKTAA
                     NKAYRGLVQDSEEQKAFQQISWLSFEKGKVSSVDWFGYVFSDKRMKSPPAFDALNGSS
                     GENNLFGTDTENNRHFTLYSAERSANKDLNLADPQIVKRMNPMHYLDNPNAAEHWRIR
                     VGTADRDTSLAISAILAIKLQMAGKNVNYETPWNVPHSGDYDVNELFLWADELVKGKK
                     "
     gene            1438..3288
                     /locus_tag="PXD81_001415"
     CDS             1438..3288
                     /locus_tag="PXD81_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HDL5537435.1"
                     /translation="MQVKKFSQKYINWVLKLGRLKASLLGFFMVALSAILIQSSLTYL
                     FIGRVVVVDILRSIAFGLISAPFVLYFFTLLVEKLEISRLKLEKAVRDLEELREQDAY
                     LNAKLEKNSRDKSILMATISHELRTPLNGIIGLSQILLDDKTTEKQQEYLKTINMSAT
                     ALAHIFGDIIDLEKIDSSRIELVRKEVEFEQLIHDIANFGHFMAGNKQINFEVKYPPD
                     LPKYIYIDHARLSQILWNLISNAVKFTPASGRILLEIYQLDKEQFSFAVTDSGIGIPE
                     KDQRKIFDMFYQAENSKEKKAQGSGIGLSVSKRIAKLMGGDLVVASQENQGSTFTLTV
                     QAESTETKPIFAIQQHSLKVLLVEDIEMNVVVARAMLEKIGCVVDVAMSGEETFQHFA
                     QNSYDLILLDIQLPDTTGFEIAQQLRLQYENEEIDYLPLLVALTANVIQSRQEYQAQG
                     MDDVLHKPLSLDELMRCLNQHFGDDFLQKNEEKQPLVERGSENLPYSITILNELIGVM
                     GKENVLANMDLFADLMPTYLANLQRYLSDWQTTNSVESRKLTADEAHKIKGALASVGL
                     HQLQQTAQLAQSDNGEQWEQNIAHWVEMICSKWQMDLQLAKEYVRSKGDI"
     gene            complement(3384..3938)
                     /locus_tag="PXD81_001416"
     CDS             complement(3384..3938)
                     /locus_tag="PXD81_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005604606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HDL5537436.1"
                     /translation="MKSQIYYDYYPSPIGQLLLLATEEGLIYIELGQEQQTTSLDNFL
                     SAQAASGQILEIFCKTKEILDRYFAGEPLDFQQLDFLAPQGTPFQQSVWKILREIPYG
                     QTTTYGEIATQLGKPSAMRAVGGAVGRNPISILVPCHRVLGKNQALTGFGGGLPNKRF
                     LLQLEDITYKDKGIEFVKPKTKKW"
     gene            complement(4005..5486)
                     /locus_tag="PXD81_001417"
     CDS             complement(4005..5486)
                     /locus_tag="PXD81_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HDL5537437.1"
                     /translation="MKMKNFSKTLIASLVAFSSAGASAAAFQLAEISTSGLGRAYAGN
                     AAVADNASVVATNPALMTLFKQTEISAGGIFVDADVNIDGKLNATGADASHKNVIPTA
                     IVPNAYIVSPINERLSIGGGVNVNYGLISEFDPNYSAGFLGGRTSLSAVNVNFSAAYD
                     LGYGFVLGAGLNAVHAKAELGRFLGAGAARFAPAKQFLEDKKVQVQSAIQGLKALEAG
                     IAAGNTALEAQKTQVLAQVNGLLKSQNLNQTITNSAQLERLVNGIQNTNASSTIHKLK
                     GDKWALGWNVGLVYNINERNRLGFTYHSEIDLKFKGKYSNTVPVVIPKMVDTITGGAE
                     IDGTLRLRLPAFWEVSGYHKLTDKLAAQYSYKRTDWSKFQSLEAYDMQGNRLLHKTEN
                     FNDSQRIALGLSYDVNEMLTLRTGVAFDESASVTHPSISIPDTDRAWYSVGATVRFTP
                     NLSADVGYSHLRGSNLTFSEDGLATFKSKARANLYGLNVNYKF"
     gene            5834..6286
                     /gene="iscR"
                     /locus_tag="PXD81_001418"
     CDS             5834..6286
                     /gene="iscR"
                     /locus_tag="PXD81_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HDL5537438.1"
                     /translation="MKLTSKGRYAVTAILDIALHGKLEPVSLADISDRQAISLSYLEQ
                     LFAQLRRDGIVQSVRGPGGGYQLGKKPEEISVGMIISAVNENIDVTKCKGSGNCRKDS
                     QCLTHSLWKRLEDQIEQFLGTISVADLAAEHADHDCEKEHCHDHPHHH"
     gene            6341..7561
                     /locus_tag="PXD81_001419"
     CDS             6341..7561
                     /locus_tag="PXD81_001419"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IscS subfamily cysteine desulfurase"
                     /protein_id="HDL5537439.1"
                     /translation="MKLPIYLDYAATTPMDERVAKKMMEYMTKDGIFGNPASRSHKFG
                     WEAEEAVDVARNQIADLIGADSREIVFTSGATESDNLAIKGAAHFYQTKGKHIITVKT
                     EHKAVLDTCRQLEREGFEVTYLDPEEDGILDLAKLKAAIRPDTILVSVMHVNNEMGVI
                     QPIKEIGAICRAKKIIFHVDATQSVGKIEVNVQDLNVDLMSFSSHKLYGPKGIGGLYV
                     CRKPRVRLEAIIHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMARLTVLRDR
                     LYNGFKDIEEVYVNGSMEEGKRIGSNLNISFNFVEGESLMMSLRDIAVSSGSACTSAS
                     LEPSYVLRAIGRDDELAHSSIRFTLGRWTTEEEIDHTIEIVKKAIVKLRELSPLWEMF
                     KEGVDLSKIEWNHH"
     gene            7688..8071
                     /gene="iscU"
                     /locus_tag="PXD81_001420"
     CDS             7688..8071
                     /gene="iscU"
                     /locus_tag="PXD81_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HDL5537440.1"
                     /translation="MAYSEKVIDHYENPRNVGTFDKEASDTGTGMVGAPACGDILRLQ
                     IKVNDQGIIEDARFKAYGCGSAIASSSLITEWVKGKSLEEAGAIKNSDIAEELELPPV
                     KVHCSILAEDAIKAAIADYKEKQAK"
     gene            8180..8503
                     /gene="iscA"
                     /locus_tag="PXD81_001421"
     CDS             8180..8503
                     /gene="iscA"
                     /locus_tag="PXD81_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012340699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HDL5537441.1"
                     /translation="MSITLTEAAANRVRTFLENRGKGIGLRLGVKTSGCSGLAYVLEF
                     VDVLNEDDQVFEQHGVKVIVDEKSLTYLNGTQLDFVKEGLNEGFKYTNPNVKNECGCG
                     ESFSV"
     gene            8513..9034
                     /gene="hscB"
                     /locus_tag="PXD81_001422"
     CDS             8513..9034
                     /gene="hscB"
                     /locus_tag="PXD81_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly co-chaperone HscB"
                     /protein_id="HDL5537442.1"
                     /translation="MNNPFTLFDLPVQFQLDNAQLSERYLALQKQLHPDNFAHKSSAE
                     QLEAVQKSADVNEALNILKDPILRAEAMIEIHTGEAKNLEEKSMRDVDFLMQQLEWHE
                     KLETIEHRKDETELTVFLKQIKAEQKAVLEQLEQTLNTADWQKANALTDKLRYFKKLI
                     IQIEKVEEKFFDY"
     gene            9088..>9710
                     /locus_tag="PXD81_001423"
     CDS             9088..>9710
                     /locus_tag="PXD81_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2625 family protein"
                     /protein_id="HDL5537443.1"
                     /translation="MKNLEQLLDKQSAWLILRDWFTQAKNHYEILASYPEDAGSALVG
                     MQLSTRSPLGAMVYETGGVFIDHGWLRILGSGNEKLPRGLFDWNFGKTFKQSGEQPSH
                     LLVADDAIGGYFAINAGGIGSDLGKVYYYHPKAKKWENTQLGYSEFLGWALAGDLNHF
                     YQDLRWENWQTDVEKLQGHQVLDLTSKIAVPIERHYQGEFAPENMGYS"
BASE COUNT         3094 a         1735 c         2085 g         2796 t
ORIGIN      
        1 gcaaaaatga accaggcaga cgcgatgaga tgatcgactt catcttttga taaactcttt
       61 gcgatttaaa cagaaaatat atttagaatg taaacaaaca tgagtcgatt ttatgttcaa
      121 agagctgtaa taagacggaa taatgtcaga ccaagataga tactttcaag taaaatcaag
      181 cataaaaatg attttcagac tttacaaatc tgaaagaatg tgtaagatca ggattgtccg
      241 aaattcggat ggaaaatccc agtggatgta ttggctggat acatcataat aactatgtat
      301 ttgtgaatgt tatttgaatg attctacgta gttgattcaa aatcagcagt gcggtatctg
      361 catgctaatg atgaaaaaat gccgggcgat gcaaataaaa ttatctctaa cggcaccagt
      421 gccggaggag cattatctac tttgttagga gcttcagctg atcattacga ctatgagcct
      481 tatttgaaag aggcgggggc attgaatgcc agtgacaaaa tttttgcagt ttctgcctat
      541 tgcccaatta ccaaccttga aaatgcggat atggcgtatg aatggcaatt taatggtgtc
      601 aatgaatata gccgaatcga tatgagtcga ttaaatgcag cagagtttaa cgaccgctca
      661 aaaccaaaac caaagccgaa aattgaaggc tcgttaaatg aggcggaaat taaagtttct
      721 aatgaattag cagaacgctt tccgacttat ttgaacagtt tgcatttggt tgatgagaaa
      781 ggcaatcctt taacacttga tccaaaaggc aacggctcgt ttaaagatta tttatctgaa
      841 gtggtaaaaa cggctgcaaa taaagcctat agaggtttag ttcaagatag cgaagagcaa
      901 aaagcctttc aacaaattag ttggctttca tttgagaaag gcaaagtcag cagtgtggac
      961 tggtttggtt atgtgttttc agataaacgg atgaaatcac cacctgcatt tgatgcgtta
     1021 aatggaagtt cgggcgagaa taatttattt ggcacagaca cggaaaataa ccgccatttt
     1081 acgctttata gtgcggaacg ttctgccaat aaagatctca atttagccga tccgcaaatc
     1141 gtgaaacgga tgaacccgat gcactatttg gataacccaa atgctgcgga acattggcgg
     1201 attagggtgg gaacggcaga tagagatacc tcgctggcaa tttctgcaat cttagcaata
     1261 aaactgcaaa tggcaggcaa aaatgttaat tatgaaaccc cgtggaatgt accccattca
     1321 ggtgattatg atgtaaatga attattccta tgggctgatg agctagtcaa aggcaaaaag
     1381 taaaaaatag agtaaaataa agcagatctg atggtctgct ttttgttttg aggtcgtatg
     1441 caagttaaaa aatttagcca aaaatatatt aattgggtat taaagctagg gcggttaaaa
     1501 gcttctcttt taggcttttt tatggtcgct ctttcggcaa ttttaattca aagtagctta
     1561 acctatcttt ttattgggcg agtagtcgtg gtggatattt tacgctccat cgcttttggt
     1621 ttaatttctg cgccttttgt gctttatttc ttcacgttat tggtggaaaa acttgaaata
     1681 tctcgcttga aattagaaaa agcagtaaga gatttggaag aactccggga acaagatgcc
     1741 tatcttaacg ctaaattaga aaaaaacagc cgtgataaaa gcattttaat ggcgaccatt
     1801 agtcacgaac ttcgtactcc ccttaatgga attatcggct tgagccaaat tttgttagat
     1861 gataaaacca ccgaaaaaca gcaagaatat ctaaaaacta tcaatatgag tgccactgca
     1921 ttggctcata tttttggcga tattattgat ttagaaaaaa ttgatagtag ccgaattgaa
     1981 ttggtaagaa aagaggtgga atttgaacaa ctcattcacg atattgctaa ttttgggcat
     2041 tttatggcgg gcaataagca gattaatttt gaagtgaagt atccgcctga tttgccgaag
     2101 tatatctata tcgaccacgc acgcttgagc caaattttat ggaatttgat cagcaatgcg
     2161 gtgaaattta cccctgcaag cggtcgaatt ttgctggaaa tttaccaatt agataaagaa
     2221 cagtttagct ttgcggtgac ggattcaggc atcggtattc cggaaaaaga tcaacgcaaa
     2281 atttttgata tgttctacca agcggaaaac tctaaagaga aaaaagcgca aggtagcggg
     2341 attggcttat cggtctcgaa acgcattgcg aagctaatgg gcggtgattt ggtggtggca
     2401 agccaagaaa atcaaggctc aacttttacc ctcactgtgc aggcagaaag cacggaaacc
     2461 aagccgatat ttgctattca gcaacattcc cttaaggtgc tattggtaga agacattgaa
     2521 atgaatgtgg tggttgcccg agcaatgttg gagaaaatcg gttgcgtggt tgatgtagca
     2581 atgagtggtg aagaaacgtt ccaacatttt gcacagaaca gctacgatct gattttactt
     2641 gatattcaac tgccggatac caccggtttt gaaattgcac agcaattaag attgcaatat
     2701 gaaaacgagg aaattgacta tttaccgctg ttggttgctt tgacggcaaa tgtgatccaa
     2761 agccggcagg agtaccaagc acaaggtatg gacgatgtgc tacacaaacc gctctcttta
     2821 gatgaattaa tgcgttgtct caaccaacat tttggagatg attttttgca aaaaaatgaa
     2881 gaaaaacagc cgcttgtaga gagaggttct gagaatttac cttatagcat tacaattctt
     2941 aatgaattga ttggggtaat ggggaaagag aatgtgcttg ccaatatgga tttatttgct
     3001 gatttaatgc ccacttattt ggcaaattta cagcgttatt taagtgattg gcaaaccaca
     3061 aattctgtgg aaagccgtaa attaacggcg gacgaggctc ataagattaa gggagcatta
     3121 gcatcagtcg gattacatca gctacaacaa actgctcagc ttgctcaatc cgataacggc
     3181 gagcagtggg agcaaaatat tgcacactgg gtggaaatga tttgttccaa atggcagatg
     3241 gatttgcaat tagctaagga atatgttaga agcaaggggg acatttagta aatttgaaat
     3301 gttacgatgg tgcaagcgtc cacgcttgta ccaattttaa tatctgaagc acaagcgtgg
     3361 acgcttgtgc tatcaaaacg tatttaccat ttcttggttt tcggttttac aaattcaatg
     3421 cctttatctt tataggtaat atcttccagc tgtaatagaa aacgcttatt cggcaagcca
     3481 ccaccgaatc cggttaaggc ttgatttttc cctaataccc tatggcaagg cactaaaatc
     3541 gaaatcgggt ttcgcccaac tgcaccgccg acggctcgca tcgcacttgg cttgcctagt
     3601 tgggtggcaa tttcgccata agtagtagtt tgcccgtaag ggatttcacg caaaattttc
     3661 caaactgatt gctggaatgg agtgccttgc ggggcaagaa aatccaactg ctgaaaatcc
     3721 aatggctcgc cggcaaaata gcggtctaaa atttctttgg ttttgcaaaa aatttctaaa
     3781 atctgaccac ttgcagcttg agcggaaagg aaattatcaa gcgaggtggt ttgttgctct
     3841 tgccctagct caatgtaaat caagccttct tctgtggcta aaagcaataa ttgcccgata
     3901 gggcttgggt agtaatcgta gtaaatttga gatttcatgt ttttatgcca ataaaaaaag
     3961 aggtgtaatt gattttacac ctctttaaac cttaattcat caaattagaa tttgtagttt
     4021 acgtttaaac cgtaaagatt tgcacgagct ttgctcttga atgtggctaa accgtcttca
     4081 gagaaggtca ggttgctacc acgaagatgt gagtaaccta catcagcaga taggtttggc
     4141 gtgaaacgca cggttgcacc tacagaatac caagcacgat cagtgtccgg aattgagatt
     4201 gatggatgag ttacacttgc actttcatca aatgctacac ctgtacgcaa tgttagcatt
     4261 tcattcacat cataagacaa gcctaatgca atacgttgag agtcattaaa gttctcggtt
     4321 ttgtgcaata ggcggttacc ttgcatatca taagcttcta ggctttggaa tttgctccag
     4381 tctgtgcgtt tatagctgta ttgtgcagca agtttatcgg ttagtttgtg gtaacctgac
     4441 acttcccaga atgccggtag gcgtaatctt agtgtaccat caatctctgc accgccggta
     4501 atggtatcta ccatcttagg aataactaca ggaacggtat tagaatattt gcctttaaat
     4561 ttaagatcaa tttcagagtg gtaagtaaaa cctaaacggt ttctttcatt gatattataa
     4621 accaagccaa cattccaacc caatgcccat ttatcgcctt taagtttatg aattgttgaa
     4681 cttgcattgg tattttgaat gccattaacc aaacgttcta attgagcaga attcgtgatg
     4741 gtttggttta aattttgaga ttttaataaa ccgtttactt gagctaatac ttgtgttttt
     4801 tgagcttcaa gagcggtatt acctgctgcg atacctgcct caagagcttt taaaccttga
     4861 attgcacttt gtacttgtac tttcttgtct tccaaaaatt gtttagctgg agcaaaacgt
     4921 gccgcaccgg cacctaagaa acgtcctaat tctgcttttg cgtgtactgc attcaaacct
     4981 gcccctaata caaaaccata acctaaatca taagccgcac taaaattaac attcacagca
     5041 cttaatgatg tacgaccacc taagaaacca gcagaatagt tagggtcaaa ttcagatatc
     5101 aaaccataat ttacatttac gccaccacca atagaaaggc gctcattgat aggtgaaacg
     5161 atataagcat ttggaacaat agcggtagga atgacatttt tatgtgaggc atctgcacct
     5221 gtagcattga gctttccatc aatatttaca tcagcatcta cgaaaatacc accggcagaa
     5281 atttcggttt gtttaaataa cgtcattaac gcagggttgg ttgcaacaac agaagcgtta
     5341 tcggctacag cggcattacc tgcgtaagct ctacctaaac cggatgttga aatttcagct
     5401 aattggaatg cggcagcaga tgcaccggct gagctgaatg ctactaatga agcgattaaa
     5461 gttttgctaa agtttttcat tttcatagga aatctcttaa aaaatatata ttttagaatg
     5521 aataattcat aaaagttata agaagaaagt gcagatttta tgtgtcgaag tactctctat
     5581 caattaaatt tcaactgttc cgatcagtta ggagcaaata agagtgaaat tgtgcggtct
     5641 atgaactgtt tatctttggt attttggtat tttcggctct tttttcgtat aatcagcgtt
     5701 attgttttaa tctgttttaa tatataaaac cttttacact aattagtcct aaatgattga
     5761 ctaaattggt ggggtatatc aaaatttagc aactgtttca gaagattttc tggataatca
     5821 atgaggatat tttatgaagc ttacctcaaa agggcgttat gcagtaaccg ccattttaga
     5881 tattgcttta cacggaaaac tagagccggt tagcttagcc gatatttcag atcgccaagc
     5941 gatttcactg tcttatttag agcaactgtt tgctcaactt cgccgtgacg gtatcgtgca
     6001 gagcgttcgt ggaccgggtg gtgggtatca attaggtaaa aaaccggaag agattagcgt
     6061 gggtatgatc atctccgccg tgaatgaaaa tattgatgtc accaaatgta aaggtagtgg
     6121 taattgccgt aaagattctc agtgtttaac gcattctttg tggaagcggt tagaagatca
     6181 aattgagcag tttttaggga caatttcagt tgcggattta gccgcagaac acgctgatca
     6241 cgattgtgaa aaagagcatt gtcacgatca tcctcatcat cactaattgc aaatttttta
     6301 tcaaaaatga ccgcttgggt tgttttaact aggagtaaca atgaaattac caatttattt
     6361 ggattacgca gcaaccacac caatggacga acgtgttgcg aaaaaaatga tggaatatat
     6421 gaccaaagac ggtatctttg gtaaccctgc ttctcgctca cacaaatttg gttgggaagc
     6481 ggaagaagcg gttgatgtgg ctcgtaacca aattgccgat ttaatcggtg cagacagccg
     6541 tgaaatcgtt ttcacttctg gtgcaacaga gtcggataac ttagccatca aaggtgcggc
     6601 tcatttctat caaaccaaag gtaaacacat tatcactgtt aaaaccgaac ataaagcagt
     6661 attagatact tgccgtcagt tagagcgtga aggttttgaa gtgacttact tagaccctga
     6721 agaagacggt attttagatt tagctaaatt aaaagcggct attcgtccgg acacaatttt
     6781 agtttcggta atgcacgtaa ataacgaaat gggcgtaatc caaccgatta aagagatcgg
     6841 tgcaatctgc cgtgcgaaga aaatcatttt ccacgttgat gcgacccagt ctgttggtaa
     6901 aatcgaagtg aatgtacaag acttaaatgt tgatttaatg tctttctcaa gccacaaatt
     6961 atacggacca aaaggtatcg gcggtctata tgtttgtcgt aaaccacgtg tgcgtttaga
     7021 agcaattatt cacggtggcg gtcacgagcg tggtatgcgt tcgggtacat tgcctgttca
     7081 ccaaattgta ggtatgggtg aagcatatcg tatcgctaaa gaagaaatgg caacggaaat
     7141 ggctcgttta accgtgttac gtgatcgttt atataacggc tttaaagata ttgaagaagt
     7201 atatgtaaac ggttcaatgg aagaaggcaa acgtatcggt tcaaacctaa acatcagctt
     7261 taactttgtt gagggcgaat cgttaatgat gtcattacgt gatatcgcag tatcatcagg
     7321 ttcagcttgt acttctgcaa gtttagagcc atcttatgtg ttacgtgcaa tcggtcgtga
     7381 cgatgaatta gctcacagct caatccgttt cactttaggt cgttggacga ctgaagaaga
     7441 aatcgatcat actattgaaa tcgtgaaaaa agctatcgtg aaattgcgtg agctttcgcc
     7501 tctttgggaa atgttcaaag aaggtgtaga tttgtctaaa atcgaatgga atcaccacta
     7561 attcagaaaa attttgcaag ggcaaataat atttgcccct acaaaataag ctttcatcac
     7621 aaatagtaaa ttgaatgtag gggcgaaata ggtttcgccg gaaataggaa ataaaaggaa
     7681 tacaaaaatg gcatatagcg aaaaagtaat tgatcattac gaaaacccac gcaacgtagg
     7741 gacttttgat aaagaagcat cagacaccgg tacaggtatg gtgggagcac cggcttgtgg
     7801 tgatatttta cgtttacaaa ttaaagtgaa cgatcaaggt atcattgaag atgcacgctt
     7861 taaagcatac ggttgtggtt ctgcaatcgc atcaagttct ctcattaccg aatgggtaaa
     7921 aggtaaatca ttagaggaag caggtgcgat taaaaacagt gatatcgctg aagagttaga
     7981 attaccaccg gtaaaagttc actgttcaat cttagcagaa gatgcaatca aagctgcaat
     8041 tgcagactac aaagagaaac aagctaagta atatgataca atcaatgcaa gcggtcgatt
     8101 tttgcaaaaa atttgcaaaa tttaaccgct tgcatttgct atttctatct tgatttaatc
     8161 cattcacata aggaaaacta tgagcattac cttaacagaa gctgccgcaa accgagttcg
     8221 tacattctta gaaaacagag gtaaaggtat cgggctaagg cttggtgtga aaacttccgg
     8281 ttgttcgggg cttgcctatg tgcttgaatt tgttgatgta ttaaatgaag acgatcaggt
     8341 tttcgaacaa cacggcgtaa aagttattgt cgatgaaaaa agcctgactt acttaaacgg
     8401 tacgcagctg gattttgtga aagaaggctt aaacgaaggc tttaaataca ccaacccgaa
     8461 tgttaaaaac gaatgcggct gtggcgaaag ttttagtgtg taaggaactg taatgaataa
     8521 cccttttact ttatttgatt tacccgttca atttcaactt gataacgccc agctttctga
     8581 gcgttatctt gctttacaga aacagcttca cccagataac tttgcccaca aatcaagtgc
     8641 tgaacagtta gaagcggtgc aaaaatctgc tgatgtcaat gaggcactca atattcttaa
     8701 agatccgatt ttacgggcag aagcgatgat tgaaatccat acaggtgaag ccaaaaatct
     8761 cgaagaaaaa agtatgcgag atgtggattt cttaatgcag caactcgaat ggcacgaaaa
     8821 actggaaacc attgagcatc gtaaagacga aaccgaactg accgtctttt taaaacagat
     8881 taaagcggaa caaaaagcag tattagaaca gcttgaacaa acgttaaata cagccgactg
     8941 gcaaaaagcc aatgcgttaa ccgacaagct ccgttacttt aaaaaattaa ttattcagat
     9001 tgagaaagta gaagaaaaat tctttgacta ttaatcctac atttgtatgg ttttcgcaag
     9061 cggtcaaatt ctaaggaaat tttgcaaatg aaaaatttag aacaattatt agataaacaa
     9121 tcggcttggt taattttaag agactggttt acccaagcca aaaatcatta cgaaatttta
     9181 gcgagttacc ctgaagatgc cggcagtgca ttggttggta tgcaactttc tactcgctca
     9241 ccgctagggg caatggtgta tgaaaccggt ggcgtattta ttgaccacgg ttggttacgc
     9301 attttaggct caggcaacga aaaattgcct cgaggcttat ttgattggaa ctttggcaaa
     9361 acttttaaac aatctggcga acaaccaagc cacttgctgg tagcggatga tgcaatcggt
     9421 ggctattttg ccattaatgc cggcggaatc ggctcagatc ttggcaaagt ttattattat
     9481 catccaaaag ctaaaaaatg ggaaaacacc caattaggtt attcagaatt tctaggctgg
     9541 gcattagccg gagatttaaa ccatttttat caggatttgc gttgggaaaa ctggcaaacc
     9601 gatgtagaga aattacaagg gcatcaagtg ctggatctta caagtaaaat cgccgttccg
     9661 attgaacgcc actaccaagg cgaatttgcc cctgaaaata tgggatattc
//
LOCUS       DANLYI010000083         9661 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.083, whole genome shotgun sequence.
ACCESSION   DANLYI010000083 DANLYI010000000
VERSION     DANLYI010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 9661)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9661
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.083"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            29..1507
                     /gene="fruB"
                     /locus_tag="PXD81_001424"
     CDS             29..1507
                     /gene="fruB"
                     /locus_tag="PXD81_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HDL5537444.1"
                     /translation="MLNLSAKNIRLNGSASNKEEVIRFVAAELVAKGNVAAGYEVGML
                     NRETQTSTFLGNGIAIPHGTLDTRDLVKETGVQIIQLPQGVEWGEGNTAYVVIGIAAK
                     SDEHLTLLRQLTTVLSDEEAAATLAKTQNLDEFIAVLSGKKNLPALSQELISLNIESS
                     SPITLSAINAAKLQEQGYVSQAFISDVVANKALHLGNNLFVVDSAKGNQANGIAIARN
                     TQGQTLLTVAETDNALQPLLTQLLKPEVRSQFAHFSAEQMLGLLKGESVASTPVTNVE
                     ASGNQVVGTFTIRNDNGLHARPAAVLVQTVKPFSSKISVENLDRNTAPASAKSAMKVV
                     ALGASQGHRLRFVAEGEDAQQAIEALQQAIINGLGESVSFVPAVADTIESIAVSAIPA
                     EETATAVDANTKEATFVIKNEHGLHARPSAVLVNEVKKFNAKIEVQNVDKNSPLVSAK
                     SLMKIVALGVTKGTTLRFVASGDDAEVALAAIGAAIEAGLGE"
     gene            1521..2465
                     /gene="fruK"
                     /locus_tag="PXD81_001425"
     CDS             1521..2465
                     /gene="fruK"
                     /locus_tag="PXD81_001425"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HDL5537445.1"
                     /translation="MRIATVTLNPAFDLVGKLQRIEIGEVNTVETLGLYPAGKGINVA
                     KVLADLGTKLSVTGFLGEENQGDFVNAFKHNDLADEFYRINGKTRINVKITETEADVT
                     DLNFLGFEISESDWEKFVTLSHQWESKFDLVAVCGSLPRGVTPEKFASWLESLHRQGL
                     KVVLDTSNAALTAGLTAHPWLVKPNRRELEVLVGHSLNSIEEIISAAKQLRSRGIENV
                     IISMGEQGSLWLNHEGVLQARPPRCENVVSTVGAGDSMVASLIYGLSQGWSKEQTLRF
                     ASATSALAVVQSNVGIADRAALDDIIKQTDITVISENH"
     gene            2491..4155
                     /locus_tag="PXD81_001426"
     CDS             2491..4155
                     /locus_tag="PXD81_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDL5537446.1"
                     /translation="MNISFVFPQTLGNARRFLVNEMLSAAAKQQGHNVVAANQADFVV
                     LFDNAIPQEAAGKQGAILDLEQAFAQPEASLKQAVENAQTFANATATTAVSAVSSGVK
                     NIVAVTACPTGVAHTFMSAEAIENYAKAQGWNIKVETRGQVGAGNPISAEEVAAADLV
                     FVAADIDVDLEKFKGKPMYRTSTGLALKKTTQEFEKAFAQAKIYEGGVAKADNAEAPT
                     SEKKGLYKHLMTGVSHMLPLVVAGGLLIAISFMFGIEAFKDETIAGGLPKALMDIGGG
                     AAFHLMIAVFAGYVAFSIADRPGLAVGLIGGMLATTAGAGILGGIVAGFLAGYTVKFL
                     NGAIQLPASLTSLKPILILPLLGSAIVGLLMIYVINPPVKALMDALVEWLNTMGQTNA
                     IILGIILGAMMCTDMGGPVNKAAYTFGVGLIASQQYMPMAAVMAAGMVPPIGMAIATL
                     IARSKFNTNQRDAGKASFVLGLCFISEGALPFVAADPVRVIASSILGGATAGAISMAL
                     GITLQAPHGGLFVIPFVSQPLMYLAAIAIGSLVTGVVYAAIKQKAE"
     gene            4322..4840
                     /locus_tag="PXD81_001427"
     CDS             4322..4840
                     /locus_tag="PXD81_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lhr family helicase"
                     /protein_id="HDL5537447.1"
                     /translation="MILLRGDTMQFIEFTDPDSGISYQYSEFTIANVAFIINFCSDAD
                     VISTLSALGKDITNYINTYSCCTIKFMAKEHLENSGSNIDIYAPAANHQFKRKEIIAL
                     QETLERLLFEHYVRFTPESYLFIAERDSLNRMYQRMCVPRCDFMQSFQVVYPLGVNQD
                     CFILITPKGNLK"
     gene            complement(4993..5856)
                     /gene="rarD"
                     /locus_tag="PXD81_001428"
     CDS             complement(4993..5856)
                     /gene="rarD"
                     /locus_tag="PXD81_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HDL5537448.1"
                     /translation="MLKGILASLAANVLFGAVYYFAILLRPLEGEAMFGFRIVVVIPF
                     ILLAIILFKQQSAFADLLQKIRQNPPLVLVILLLALNTGVQLWLFLWAPNHGQALQVS
                     FGYLLLPIVAVALGKMVFKEHFTRLKWLSLLFAVIGVGSSLFFNSTISWATWVAGLGY
                     PLYITLRRYFQINTLATFFVELLLVLPVALYYLSQVEMTPIVAENPQIYFYLGLLGLV
                     SGSAFILYILSSNLLPINVLGLLGYVEPLVMLLISFAIGEKLDANSYILMVCLLVSIG
                     LLSWDSFRSKV"
     gene            6034..6618
                     /locus_tag="PXD81_001429"
     CDS             6034..6618
                     /locus_tag="PXD81_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HDL5537449.1"
                     /translation="MKKIFWILLIAMLAVGGYSLTKYNDLMRAEEDINSVWGNVESAY
                     QRRADLIPNLVNTVKGQANFEKETLTSVIEARAKATAVTIDPSNATEEQMAKFQEAQQ
                     GVNSALSRLLVSVEKYPELKAHEAFLNLQAQLEGTENRINVERNNFNEKVKEYNKQVR
                     EFPTKLAAMIMGFKAKPQFKSEAGSDKAPTVNFN"
     gene            6700..7158
                     /locus_tag="PXD81_001430"
     CDS             6700..7158
                     /locus_tag="PXD81_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005623246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HDL5537450.1"
                     /translation="MGLFSFLSNKLPIDTQRIEQAIADLEQKTSAELRVVVERKAKIG
                     KTDNAAMLRASELFDELKMRETAEHNGVLIYLSFKPHYAAIIGDEGIHQKVDNDFWQK
                     IYATMCLDCQQGDYTQAICDAIKMVEAPLAHHFPYQADDKNELSNEVVVK"
     gene            7155..8012
                     /locus_tag="PXD81_001431"
     CDS             7155..8012
                     /locus_tag="PXD81_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HDL5537451.1"
                     /translation="MMKSLCKFLSIFNRLLLVCLALFSLNATAVTYPEPPNPFYYITD
                     YTKNTLSQQEWRTLEDALIANCAKTSSQIAVVIIPSTEGEAVSTYAHTLFNKWGIGRS
                     KNNENNGILLLIAKNDRKLFIATGRGLEGALPDAIASSIIRNDITPFFKQEQYAAGIA
                     HGLSAIMAAINGEYAPYASYGEEMQEFDDIDGLLFFLFAGIVIFLIFRPRGNSTYISP
                     SSMDQLGQILRESQRRGGGFGGGLGGGFDRGSGGFGGGFGGGFGGSRGSGDSFGGGSS
                     GGGGAGGSW"
     gene            complement(8127..8606)
                     /gene="ispF"
                     /locus_tag="PXD81_001432"
     CDS             complement(8127..8606)
                     /gene="ispF"
                     /locus_tag="PXD81_001432"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HDL5537452.1"
                     /translation="MIRIGHGFDVHAFGEDRPLMIAGVEVPYHTGFIAHSDGDVALHA
                     LTDALLGAAALGDIGKLFPDTDMQYKNADSRGLLIEAYRQVQEQGYQVGNVDVTIIAQ
                     APKMRPHIDKMRQVIADDLQCDISQVNVKATTTEKLGFTGRGEGIACEAVALLLKRA"
     gene            complement(8608..9291)
                     /gene="ispD"
                     /locus_tag="PXD81_001433"
     CDS             complement(8608..9291)
                     /gene="ispD"
                     /locus_tag="PXD81_001433"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HDL5537453.1"
                     /translation="MKRKIIAVIPASGVGSRMQAEKPKQYLQLKGKTILEHTVSIFLE
                     HPHVEKVIVAVARKDPFYSTLELLKSPKIQLVFGGETRADSVLNALQEINTENAWALV
                     HDAARPCLKRSDLDKLLQINDPQGGILAIPAVDTMKRANGTKIAHTEDRSTLWHALTP
                     QFFPAQALKQALSSAFANKLQVTDEASAMELAGYQPQLVSGRSDNIKITRPEDLALAE
                     FYLTHNLEN"
     gene            complement(9309..9587)
                     /gene="ftsB"
                     /locus_tag="PXD81_001434"
     CDS             complement(9309..9587)
                     /gene="ftsB"
                     /locus_tag="PXD81_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005597256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HDL5537454.1"
                     /translation="MRLLITFFALLLGFFQYSFWFGKNGWSDYQEVQEEIALLKVENT
                     KLTSRNKLIEAEIYDLKNGVDALEERARLEREMVKENEMFYRIVPKNK"
BASE COUNT         2899 a         2083 c         2022 g         2657 t
ORIGIN      
        1 aaatcaactt taaatccaag gagctaaaat gcttaattta tcggcaaaaa acatccgttt
       61 aaatggttca gcaagcaaca aagaagaagt tatccgtttt gttgccgccg aattagtcgc
      121 caagggcaat gtagcagccg gctatgaagt cggtatgctt aatcgtgaaa cccaaacctc
      181 tactttttta ggtaacggaa ttgctattcc ccacggcacg cttgataccc gtgatttagt
      241 caaagaaacc ggcgtgcaaa ttatccaact tccgcaaggc gtggaatggg gcgaaggcaa
      301 cactgcttat gtagttatcg ggattgcggc taaatcggac gaacacttaa ccttactccg
      361 tcaactcacc actgtattaa gtgatgaaga ggctgctgcc acattagcga aaacgcaaaa
      421 tttagatgaa tttattgccg tattaagcgg taagaaaaac cttcctgcct tatctcaaga
      481 gctgatttct ctgaatatcg aatcttcaag ccctatcact ttatcagcca ttaacgccgc
      541 aaaactacaa gagcaaggct atgtttccca agcattcatc agcgatgtgg ttgccaacaa
      601 agcgttacat ttaggcaata atctatttgt ggtggattcc gccaaaggca accaagctaa
      661 cggtatcgct attgcccgta atacgcaagg tcaaacccta ctaactgtgg cagaaaccga
      721 caatgctcta cagccactat taactcaatt attaaaacca gaagtaagaa gccagtttgc
      781 tcatttctct gcggagcaaa tgcttggttt attaaaaggc gaaagcgtag cttcaacccc
      841 tgtaaccaat gtcgaagcga gtggcaacca agtggttggc acattcacca ttcgtaacga
      901 caacggctta cacgcccgcc cggctgcggt gttagtacaa acggtgaaac cgttctcctc
      961 aaaaatcagc gttgaaaatt tagaccgcaa cactgcaccg gcaagtgcga aaagtgcaat
     1021 gaaagtagta gcactagggg caagccaagg gcatcgttta cgctttgtgg cagagggaga
     1081 agacgcccaa caagcgattg aggcattaca acaagcaatt attaacggct taggcgaaag
     1141 tgtttcattc gtaccggcgg tggcagatac gattgaaagc attgctgttt cagcaattcc
     1201 tgcggaagaa actgcgactg ctgtcgatgc caatacgaaa gaagcaactt ttgtaattaa
     1261 aaacgaacac ggtttacacg ctcgcccaag tgcggtgttg gtgaatgaag tgaaaaaatt
     1321 caacgccaaa attgaagtgc aaaatgttga taaaaattca ccgcttgtaa gtgccaaaag
     1381 cttaatgaaa attgttgccc ttggcgtaac caaaggcacg acgctacgct ttgtggcaag
     1441 cggtgatgat gccgaagtag cacttgcggc aatcggtgct gcgattgaag caggtttagg
     1501 cgaataagcg aggaatcatt atgcgtattg caaccgttac cctcaaccct gcctttgatt
     1561 tagtcggcaa acttcaacgt attgaaatcg gcgaagtgaa caccgtagaa acccttggtt
     1621 tataccctgc cggcaaaggt attaacgttg ctaaagtttt agctgactta ggcacaaaac
     1681 tttcggttac cggcttttta ggcgaagaaa atcaaggcga tttcgtaaat gcctttaaac
     1741 acaatgattt ggctgatgaa ttttaccgta ttaacggtaa aacccgcatt aatgtcaaaa
     1801 tcactgaaac cgaggctgat gtaactgatc tgaactttct cggctttgaa atttcggaaa
     1861 gcgattggga aaaattcgtc acattatctc atcagtggga aagcaaattt gacttagttg
     1921 cagtgtgtgg atctctccct cgtggcgtaa cgccggaaaa atttgcttct tggttggaat
     1981 ctttacaccg ccaaggctta aaagtggtgt tagataccag caatgcggct ctcaccgccg
     2041 gcttaaccgc tcacccttgg ttagtaaaac caaaccgccg agagctggaa gtgctggtcg
     2101 ggcattcact caactcaatc gaagagatta ttagtgcagc taaacaatta cgttcccgtg
     2161 gtattgaaaa tgtaattata tcaatgggcg aacaaggctc tctttggcta aatcacgaag
     2221 gcgtgttaca agcccgccca ccacgttgcg aaaatgtagt tagcactgta ggtgctggtg
     2281 attcaatggt tgccagcttg atctacggct tatctcaagg ttggagcaaa gagcagacct
     2341 tgcgttttgc ctcagctact tctgctctag ctgttgttca aagtaacgtt gggattgcag
     2401 accgtgcggc gttagacgat attattaaac aaaccgacat taccgtgatt tcagaaaatc
     2461 attaaacctc aatcaaagga acaaaccaat atgaatattt cttttgtatt tcctcaaaca
     2521 ctaggcaacg cacgccgttt cttagtgaat gaaatgttat ctgccgctgc caagcaacaa
     2581 ggacacaatg ttgtggcggc aaaccaagct gattttgtgg tgttattcga taacgcaatt
     2641 ccacaagaag ctgccggcaa gcaaggggca attttggact tagaacaagc ctttgctcaa
     2701 cctgaagcca gtctcaaaca agcggtcgaa aatgcacaaa cttttgcaaa tgctaccgca
     2761 accactgcgg tttcagcggt ttcatctggt gtgaaaaata tcgttgctgt taccgcttgc
     2821 ccaactggcg tggctcatac ttttatgtct gcggaagcaa tcgaaaatta tgccaaagca
     2881 cagggttgga acatcaaggt tgaaacccgt ggtcaagtag gagcaggaaa tccaatttcc
     2941 gccgaagaag tcgcagcggc agatttagtc tttgtagctg ccgatattga tgtcgatttg
     3001 gaaaaattca aaggcaagcc gatgtatcgc acctcaaccg gtttagcatt gaagaaaacc
     3061 actcaagaat ttgaaaaagc ctttgctcaa gcgaaaattt acgaaggtgg tgtggcaaaa
     3121 gcggataatg ctgaagctcc aacttctgag aaaaaagggt tatataaaca cttgatgacc
     3181 ggtgtatcac atatgttacc gctggtggta gcaggtggct tgctcatcgc tatttccttt
     3241 atgttcggaa ttgaagcctt taaagatgaa accatcgcag gcggcttgcc gaaagcctta
     3301 atggatattg gtggcggtgc ggcattccac ttaatgattg cggtattcgc aggctatgta
     3361 gcgttctcaa ttgcagaccg tccggggtta gctgtcggtc ttatcggcgg tatgcttgct
     3421 accaccgcag gagcagggat tttaggtggt atcgtagccg gtttcttagc cggttatacc
     3481 gtaaaattct taaatggtgc gattcagcta ccggcaagtt taacctcact taaacctatt
     3541 ttgattttac cactcttagg ctcagcgatt gtcggcttac tgatgattta tgtcatcaac
     3601 ccaccggtga aagcattaat ggatgcctta gtggaatggt taaacacaat ggggcaaacc
     3661 aatgcaatta ttttaggtat tattctcggt gcgatgatgt gtaccgatat gggtggtccg
     3721 gtgaataaag cggcttatac ctttggtgtg ggtttaattg cttcacaaca atatatgcca
     3781 atggcagcgg tgatggcggc aggtatggtt ccaccaattg gtatggcgat tgcgaccctg
     3841 attgctcgta gcaaattcaa caccaaccaa cgtgatgcag gtaaagcttc attcgtgtta
     3901 ggtttatgct ttatttcaga aggggcatta ccgttcgtgg cggcagaccc tgtgcgtgta
     3961 attgccagct caatcttagg cggtgcaacc gccggtgcaa tttcaatggc actaggcatt
     4021 accctacaag ccccacacgg cggtttattc gtgattccat tcgtttcaca accgttaatg
     4081 tacttagctg ctattgccat cggctcatta gtcaccggcg tagtctatgc cgctatcaaa
     4141 caaaaagcag aataaagcac aaacacaaat acaaacaaaa acggagcgaa acgctccgtt
     4201 tttatatcat attggtactt actgaattct atttcatcga aatatcaagt ctaaaatatt
     4261 atttttgcga tcgagatcga aaaaatcatt ttcatcactt tacgaataat atcaactcgt
     4321 tatgattttg ctacgaggag atactatgca gtttattgaa tttaccgatc ctgatagtgg
     4381 aatttcttat caatacagtg aatttactat tgctaatgtc gcatttatca ttaatttctg
     4441 ctcagatgcc gatgttatat cgactttgtc cgcattaggc aaagatatca caaactatat
     4501 aaacacctat tcctgttgca caatcaaatt tatggcgaaa gagcatctag aaaatagcgg
     4561 ttctaatatt gacatttatg cccccgcagc gaatcatcag ttcaaacgta aagaaataat
     4621 tgctttacaa gaaacccttg agcgtttatt atttgagcat tatgtacgtt ttacaccaga
     4681 aagctatctc ttcattgccg agagagattc acttaaccga atgtatcagc gtatgtgtgt
     4741 gccacgatgt gattttatgc aatctttcca agtggtttat cccttgggcg ttaatcaaga
     4801 ttgtttcatt ttaattacgc caaaaggaaa tctaaaatga atcaacaacg tccagtttcc
     4861 gttcgtttaa agcaagaagg cttaatcgct tttaagcaag cctatcagcg agcaaaaaaa
     4921 gcaggtctat tgaaaccggt caaataatct ttaaaccgct tgctctacaa gcgatttatt
     4981 tttccttaat tatcacacct tgctacgaaa actatcccaa ctcaataatc caatcgaaac
     5041 cagcaaacag accatcaaaa tataggaatt ggcatcgagt ttttcgccga tggcaaaaga
     5101 aatcagcaac atcaccaacg gttcaacata gcccagtaaa cctaatacat taatcggtaa
     5161 taaattgctc gagaggatat aaagaataaa agcactgcca cttaccagcc ccagcaatcc
     5221 gaggtagaaa taaatttgcg gattttctgc cactattggc gtcatttcca cttggctgag
     5281 ataataaagt gcaacgggta ataccagtaa caactccaca aaaaaagtag ctaacgtgtt
     5341 gatttgaaaa taacggcgaa gggtaatata aagcggataa cccaacccag ctacccaagt
     5401 cgcccacgaa atcgtgctgt taaaaaacaa actactgcca acaccgatga ctgcgaaaag
     5461 tagagaaagc catttcaggc gggtaaaatg ttctttaaac accatttttc ctagagccac
     5521 cgccacaatc ggtaaaagca agtaaccaaa cgacacctgc aacgcctgcc cgtgattagg
     5581 agcccataaa aacagccaaa gctgcacgcc ggtgttgaga gcaaggagca aaatgaccaa
     5641 tacaagcggt ggattttgtc gaattttttg caaaagatcc gcaaaagccg attgctgttt
     5701 aaacaatata atcgccagca aaataaacgg aatgactacc actatccgaa agccgaacat
     5761 cgcctctccc tctaacgggc gaagcaatat cgcaaaatag taaactgctc caaacagcac
     5821 attggctgca agcgaagcta aaattccttt gagcatttgg ctttcctctt atcaacaatc
     5881 aaacaacaag cggttgactt tgaacaaaaa tttacaaatc ttatctacgc cccatagtat
     5941 gaaaaaattt gtaagtaagc tagctatatt cgatttttcc cgatacaatg ctcccaattt
     6001 ttcactcttt tatttatatt ataggacttc ataatgaaaa agattttttg gattttactg
     6061 atcgccatgc ttgctgttgg cggttattct ttaacaaaat acaacgattt aatgcgtgca
     6121 gaagaagaca tcaactccgt atggggaaat gttgaatcgg cttaccaacg ccgtgcggat
     6181 ttaattccaa acttagtgaa taccgtaaaa ggtcaggcta actttgagaa agaaacttta
     6241 acctctgtga ttgaagctcg tgcaaaagca actgcggtaa ccattgaccc aagcaatgca
     6301 actgaagagc aaatggctaa attccaagaa gcccaacaag gtgtaaactc tgctctttca
     6361 cgcttattgg tttcagttga gaaataccct gaattaaaag cccacgaagc gtttttaaat
     6421 ctacaagctc aattagaagg tacggaaaac cgcattaacg ttgaacgcaa taactttaac
     6481 gaaaaagtga aagagtacaa caagcaagtg cgtgaattcc cgactaaatt agcggcaatg
     6541 attatgggct ttaaagcaaa accgcaattt aaatctgaag caggttctga taaagcacca
     6601 accgtaaact tcaactaatt ttagtttcaa tttattcata ttaaaagcaa taagctcttt
     6661 caaatccggt gcttattgct ttatttttaa ggggaacaaa tgggattatt ttcttttctc
     6721 tccaacaaat taccgataga tacgcaacgc attgaacaag caattgccga tttagagcaa
     6781 aaaacctctg ccgaattgcg tgtggtggtg gaacgaaaag ccaaaatcgg caaaaccgac
     6841 aacgccgcta tgttgcgagc aagtgagctt ttcgatgaat taaaaatgcg tgaaacggct
     6901 gaacataacg gtgtgttgat ctacctttca ttcaagcctc actacgctgc gattattggc
     6961 gatgaaggca ttcatcaaaa agtcgataac gatttttggc aaaaaatcta tgccacaatg
     7021 tgtttggact gccaacaagg cgattacacc caagccattt gtgatgcaat caaaatggtg
     7081 gaagctccgc tcgctcacca tttcccttat caggctgatg ataaaaacga gctttcaaac
     7141 gaggtggtag tcaaatgatg aaatcccttt gcaaatttct atctattttc aaccgcttgt
     7201 tattggtgtg cttggctctg tttagcctaa atgccacagc ggtgacctac ccagagccgc
     7261 ctaatccgtt ttattatatt acggactaca ccaaaaacac cttgagccaa caagaatggc
     7321 ggacattaga agatgcctta attgctaact gtgccaagac aagttcgcaa attgccgtgg
     7381 tgattattcc aagcacggaa ggcgaggcag tttcaaccta tgctcatacc ctgtttaaca
     7441 aatggggaat cggacggagc aaaaataacg agaacaatgg tatattgtta ttaattgcca
     7501 aaaatgatcg caaactcttt attgccaccg gacgtgggct tgaaggagcg ttacctgatg
     7561 cgattgcatc gagcattatc cgcaatgaca tcacgccatt cttcaaacaa gagcaatatg
     7621 cagccggtat cgcccatggc ttatccgcca ttatggcagc cattaacggg gaatatgccc
     7681 cttatgcaag ttatggcgaa gaaatgcaag aatttgatga catagacggc ttactctttt
     7741 tcttgtttgc cggcattgtg attttcctaa ttttccgccc acgaggaaac agtacctata
     7801 ttagtcccag ctcaatggat caactaggac aaatactaag agaaagccaa cgcagaggtg
     7861 ggggctttgg cggtggtctc ggaggtggct ttgatcgagg ctcaggtgga tttggcggag
     7921 gcttcggtgg tggttttggc ggaagcagag gcagtggcga cagcttcggt ggtggttcat
     7981 caggcggtgg aggcgctgga ggaagctggt agtttccgcc caatttaaaa caatacaagc
     8041 ggtcatattt ctcaaaaaaa ttgcgtattt ttcgctaaac gcacaaaacg ctaatgaaaa
     8101 taagaccgct tcctcacttc tctaagctat gcccttttta gtaacaatgc tacggcttca
     8161 caagctatgc cttcacctcg cccggtaaac cctaattttt ctgttgtagt ggcttttaca
     8221 ttcacttgtg atatatcgca ttgtagatcg tctgcgatca cctgtctcat tttatcaata
     8281 tgcggtcgca tcttcggagc ttgagcaata atggttacat cgacgttacc gacttgatag
     8341 ccctgttctt gcacttgtcg gtaagcttca atcaacaaac cacgactatc ggcatttttg
     8401 tattgcatat cagtatcagg gaataatttg ccaatatcac ctaaagctgc tgcgccaagc
     8461 aacgcatctg ttaaagcgtg cagcgctaca tctccgtcac tatgcgcaat gaagcctgta
     8521 tgataaggca cttcaacccc agcaatcatc agtggtctat cttcgccaaa agcgtgaaca
     8581 tcaaaaccgt gtccaatacg tatcatttta gttctccaaa ttatgtgtaa ggtaaaattc
     8641 cgctaaggct aaatcttcag ggcgagtgat tttgatatta tcacttctac ctgaaaccaa
     8701 ttgtggctgg tagcctgcaa gctccattgc agatgcttcg tctgttacct gaagcttatt
     8761 ggcaaaagca gaggataatg cttgttttaa tgcttgagca ggaaaaaatt gtggggttaa
     8821 agcgtgccat aaggttgagc gatcttctgt gtgtgcgatt ttcgtgccgt ttgctctttt
     8881 catcgtatct accgctggaa tggcgagaat gccaccttgc ggatcgttaa tttgcaaaag
     8941 tttatctaaa tctgaccgct tgaggcaagg gcgagccgca tcgtgaacta atgcccaagc
     9001 attttcagtg ttgatttcct gtaaggcatt tagcactgaa tctgctctcg tttctcctcc
     9061 aaaaacaagt tggatcttag gggattttag gagttccaat gtggaataaa aagggtcttt
     9121 tcttgccacc gctacaatca ctttttcaac atgagggtgt tcaagaaaaa tagacactgt
     9181 atgctctaag atagtcttac cctttaattg taaatattgt tttggttttt ctgcttgcat
     9241 ccggctacca actcctgacg ccggaattac tgcaataatt tttcgcttca taatatatca
     9301 attatgtatt atttgttttt tggtacaatt cggtagaaca tctcgttctc ttttaccatt
     9361 tctcgttcaa gtcttgctct ctcttcaagt gcatctacgc cgttctttaa atcgtaaatt
     9421 tcggcttcaa ttagtttatt gcgtgaagta agctttgtgt tttctacttt taataaagca
     9481 atttcttctt gcacttcttg ataatcactc caaccatttt ttccaaacca aaagctatat
     9541 tggaaaaagc cgagtaatag ggcaaaaaaa gtaattaata aacgcattta ttttctctta
     9601 tatgaaatag taatgaacta agcgggttga ttttaccttg aatttgcaaa aaattctaaa
     9661 a
//
LOCUS       DANLYI010000084         9481 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.084, whole genome shotgun sequence.
ACCESSION   DANLYI010000084 DANLYI010000000
VERSION     DANLYI010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 9481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9481
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.084"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            89..913
                     /locus_tag="PXD81_001435"
     CDS             89..913
                     /locus_tag="PXD81_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5537455.1"
                     /translation="MEHFIGLGVAGNFAGHLEQAGEEADFAKVKTVEAVQPKAIFPFY
                     VPAENLGDYQFLSTYPLSNTAINFPSDADNLQIEPEVALICEIGYQDQQVVSLTPTHF
                     AAYNDCSIRRPNANKICEKKNWGASSKGLSAIHLPLDNFNKGGTLDNYNIACFHKRNG
                     ELNTYGIDSPAVEYSYFHQKLLDWIVERMNNQPDEGPMNNISEMLKQANYPTKAIISI
                     GATRYTPFGESNLLQVGDVSMVVVYNVKKYSHSQIEEMAKLETFSEDISALIQTVR"
     gene            1047..1871
                     /locus_tag="PXD81_001436"
     CDS             1047..1871
                     /locus_tag="PXD81_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase"
                     /protein_id="HDL5537456.1"
                     /translation="MKKLLLFLLFIGNPIFAKSPELMLVEQYQDQDISGWVMSEKLDG
                     VRGFWDGQQLISRQGYPLNPPDYFIQDFPPFAIDGELFSERGKFEEISSIVRSAQPKG
                     WHKLKLQVFDVPNAQGNLIERLATLENYLKQHPTLHIAIIEQIPIKDKAHLQQFYQSV
                     LEQGGEGVIVRNPNAGYIQGRSAQILKIKPVLDEECTVVAHHKGKGKYTDKLGAITCE
                     NQRGRFRIGSGFKDKERENPPPIGSIITYKYRGVTESGKPRFATFWRIRNDQISEN"
     gene            1965..2612
                     /gene="pxpB"
                     /locus_tag="PXD81_001437"
     CDS             1965..2612
                     /gene="pxpB"
                     /locus_tag="PXD81_001437"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HDL5537457.1"
                     /translation="MLTIHHTSEHSLLCLAEPPAELAKQQQIWRVAEQARALDGVLEV
                     VVGMNNFTLFHAIGTDSTRLIQAVQQLWENDQAQAAQSPRLIEIPVHYGGEFGEDLFE
                     VAQFHNTTPEEIIRRHTEPTYTVFMIGFQPGFPYLNGLPQNLHTPRRNVPRTKVPAGS
                     VGIGGRQTGIYPFTSPGGWQLLGRTELPLFDIQHNPPTLLTAGDNVRFVAESITL"
     gene            2609..3538
                     /locus_tag="PXD81_001438"
     CDS             2609..3538
                     /locus_tag="PXD81_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase family
                     protein"
                     /protein_id="HDL5537458.1"
                     /translation="MIYIEIIQGFAHIQDLGRYGYRHLGVSHGGAMDSLALQAGNLLL
                     KNANNAPAIELTLGGMRITFDCDTPFCLTGALVEAELDGTPVFSYYRYTANARQTLTI
                     KRIVLGNYAYLCVAGGFLVPQVLGSASTALKAQFGGFQGRMLKAGDNIATGRRDSRLS
                     LMSIEPIDFTSRIRATASSEYEAFTADSRERFWQQGWQLQNNSNRMGYRFAEKALELT
                     ASLEMLSYAAPSGTVQVPPDGQPIVLMADAQTTGGYPKIACIIQADLGRLAQNPFGST
                     VQFEQVSREQAVEIYQKDQNYLETIRRKANESR"
     gene            3525..4262
                     /gene="pxpA"
                     /locus_tag="PXD81_001439"
     CDS             3525..4262
                     /gene="pxpA"
                     /locus_tag="PXD81_001439"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HDL5537459.1"
                     /translation="MNHVDLNVDLAEGCGNDHSLLQLVTSANVCCGLHAGDYNEMRKA
                     INWAKENSVRVGAHPSFPDRENFGRTNMQLPDDELKACLLYQLGAAKALCDAENMPMS
                     YVKPHGALYNMAAKDPHLATLIANTVKQFDPNLKLMGLSGSLMLSIAEQHGLETISEV
                     FADRHYLADGSLVPRTRDDAMVESDDEAISQVLQMVLDGTVPTVDGGNVAIKADSICL
                     HGDGEHAVVFAERIKKELQAKGILVKA"
     gene            4537..5724
                     /locus_tag="PXD81_001440"
     CDS             4537..5724
                     /locus_tag="PXD81_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HDL5537460.1"
                     /translation="MSNNNRSALLGAAFLMATSAIGPGFLTQTATFTQTLLASFGFVI
                     LLSILLDIGAQLNIWRIVAVSEKKAQDIANAVLPGAGYFLALLIVMGGLAFNIGNVGG
                     AGLGLNILTGLSPEMGAVVSGAIAVGVFLFHEAGKVMDRFAQIMGFVMIALTIFVAVK
                     ANLPIDDAVVHTFVPEKLDVIAIVTLVGGTVGGYITFAGAHRLLDAGIKGKENLKEVS
                     KSSVSAILIASIMRVVLFLAVLGVVSMGVQLDPKNPAATPFAHVAGDVGLMIFGVVIW
                     AASITSVIGAAYTSVSFITSFSPFIEKHKNCFIVAFIVVSTAVLATIGRPAQVLVFVG
                     TLNGLILPISLGLILLAAHKTKIVGDYKHPVWMTVSGAIVVILMAVLSAMTLSKYISG
                     LMA"
     gene            5914..6168
                     /locus_tag="PXD81_001441"
     CDS             5914..6168
                     /locus_tag="PXD81_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxaloacetate decarboxylase subunit gamma"
                     /protein_id="HDL5537461.1"
                     /translation="MAPAELFAEGINLMVAGMGFVMLFLLVLIYAISFISKIINQYFP
                     EPVSIPASKTVPQSPPDDLERLRPVIAAAIAHHRRINGQH"
     gene            6208..8034
                     /gene="oadA"
                     /locus_tag="PXD81_001442"
     CDS             6208..8034
                     /gene="oadA"
                     /locus_tag="PXD81_001442"
                     /EC_number="7.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005608604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-extruding oxaloacetate decarboxylase
                     subunit alpha"
                     /protein_id="HDL5537462.1"
                     /translation="MTTQPKKIAITDVVLRDAHQSLFATRLRLDDMLPIASELDNIGY
                     WSLETWGGATFDSCIRFLGEDPWVRLRELKKAMPKTPMQMLLRGQNLLGYRHYADDVV
                     DKFVERSVANGMSVFRVFDALNDPRNMQAALQAVKKHGGHAQGTLSYTTSPVHTLQTW
                     LDVTEQLLEIGVDSLVIKDMSGILTPMAAYELVSEIKKRYDVELHLHCHSTTGMAEMA
                     LLKAIEAGVDGVDTSISSMSGTYGHPATESIVATLQGTPYETGLSIPALEKISAYFRN
                     VRKKYAKFEGQLRGIDSRILVAQVPGGMLTNLESQLKQQNASDKLDLVLQEIPRVRED
                     LGNIPLVTPTSQIVGTQAVMNVLMGERYKTIAKETAGILKGEYGRTPAPVNKALQDRV
                     LEGAEPITDRPADHLPPELEKLVAEVTASAKEKGITLSENMIDDVLIVALFQQVGWKY
                     LENRNNPSAFEPVPSADEAKPAQASAASAPKAQTQSGSAIYTVELEGKAFVVKVSEGG
                     DITNIAPTPATPAPQAVDSAPKSANTLSANAEPVNAPMAGNILKVEVKEGQQVAEGDV
                     LLILEAMKMETQICAAKSGTIQGINVKQGDVVAVNDTLMSIA"
     gene            8044..9348
                     /locus_tag="PXD81_001443"
     CDS             8044..9348
                     /locus_tag="PXD81_001443"
                     /EC_number="7.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005605232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HDL5537463.1"
                     /translation="MESIIALFKGMGLMHLELGQAIMIVISLVLLWLAIARKFEPLLL
                     LPIGFGGLMSNIPEAGMAMTALDNLLHSGSAEQLAIIAAKVNSTSLDLHSIKDAIALA
                     APSVQNELEVMAGDMGYTAGVLALFYKVAIGYGVAPLIIFMGVGAMTDFGPLLANPRM
                     LMLGAAAQFGIFATVLGALGLNWLGIIDFTLPQAAAIGIIGGADGPTAIYLASKLAPE
                     LLGAIAVAAYSYMALVPLIQPPIMKALTTEQERKIRMVQLRNVSNREKIIFPIVLLLL
                     VALLLPDAAPLLGMFCFGNLLRTSGVVERLNDTAQNALINIVTIFLGLSVGAKLVADK
                     FLQPQTLGILVLGVIAFGIGTASGVIMAKIMNRFSQHKINPLIGSAGVSAVPMAARVS
                     NKVGQEYDKQNFLLMHAMGPNVAGVIGSAIAAGVMLKYISTM"
BASE COUNT         2588 a         2139 c         2243 g         2511 t
ORIGIN      
        1 tctatcaatc agatcaaaat aaacattcgc aatttttggt tacaagcggt aaaattttat
       61 ccaactttta ccaattaagg agtagattat ggaacacttt atcggtttag gcgtggccgg
      121 caattttgct ggacatttag aacaagccgg agaggaggcg gattttgcga aagtgaaaac
      181 agtggaagca gtgcagccaa aggcgatttt cccgttttat gtgccggcgg aaaatttggg
      241 ggattatcag tttttatcca cctatccgct ttcaaatact gcgattaatt tcccgagtga
      301 tgctgataat ctgcaaattg agcctgaagt ggcgttaatt tgtgaaattg gctaccaaga
      361 tcagcaagtg gtttcgctca cgccaaccca ttttgcggct tacaatgact gctctatccg
      421 ccgtccgaat gcgaataaaa tctgtgaaaa gaaaaactgg ggggcttcgt ctaaagggct
      481 ttcggcaatt catcttccgt tagataactt caacaaaggc ggcacgctgg ataattacaa
      541 catcgcttgt ttccataaac gtaatggcga gctgaatacc tacggcatcg acagccctgc
      601 ggtggagtac agctatttcc atcaaaaatt gctcgattgg attgtagaga gaatgaataa
      661 tcagcccgat gaaggtccga tgaacaacat ttccgaaatg ttaaaacagg caaactaccc
      721 aactaaagcg attatcagca ttggggcaac acgctacacg ccttttggcg aaagtaattt
      781 attgcaagta ggtgatgtaa gtatggtggt cgtttacaat gtgaagaaat attctcatag
      841 ccaaattgaa gaaatggcga agttggaaac ctttagcgag gatatttctg ctttaattca
      901 aacagtacgt taaatttctc actcaagcga tcagtttttc ggaaaatttt gcgatctgcg
      961 tcgaaaaatt gagattcatt tctgactgtt tgcacgcttt cctttatagt gccttgcata
     1021 ttcggaggca ctatttcgga gagactatga aaaaactgct cttattccta ctcttcattg
     1081 gtaaccctat ttttgccaaa tcgcccgagt taatgctcgt tgagcaatat caagatcaag
     1141 acatcagtgg ctgggtaatg tcagaaaaac tcgatggcgt gcgaggcttt tgggacggtc
     1201 aacagctcat cagccgccaa ggttatccgc tcaatccacc cgactatttt attcaagatt
     1261 tccctccttt tgcgattgac ggcgaactgt ttagcgaacg gggcaaattt gaggaaattt
     1321 caagcattgt gcgttccgct cagcccaaag gttggcacaa gctgaaatta caggtgttcg
     1381 atgtgccaaa tgcacaaggg aatttgattg aacggctggc gaccttagag aattatctga
     1441 agcaacatcc aacgctccat atcgctatta ttgagcaaat tccgattaaa gataaagccc
     1501 accttcaaca attttaccaa tctgtgctgg aacaaggtgg cgaaggggtt atcgttcgca
     1561 acccgaatgc aggctatatt caaggtcgtt ctgcccaaat tttgaaaatc aaaccggttt
     1621 tagatgagga atgcactgtg gttgcccatc ataaaggcaa agggaaatat accgataaac
     1681 tcggggcaat cacttgtgaa aatcaacgtg gacgttttcg gattggcagc ggttttaaag
     1741 ataaagaacg agaaaatccg ccaccgattg gctcaatcat tacctataaa tatcgaggtg
     1801 tcacagaatc aggcaagccc agatttgcaa cgttttggcg aataagaaat gatcaaattt
     1861 ctgaaaatta agagctattt cacattttca acaaaataaa attgacagtc tggatatatc
     1921 agcgtaaaaa gttgttacct ttttgtaaca atttgagggc aattatgctg acgattcatc
     1981 ataccagcga acattccctg ctctgcttag ccgaaccgcc tgcagagctt gccaaacagc
     2041 aacagatttg gcgggtggca gaacaggcaa gagccttaga tggcgtgttg gaagtggtgg
     2101 taggaatgaa caattttacc ctatttcacg ccattggcac cgatagcaca cgccttatcc
     2161 aagcggttca acaactctgg gaaaacgacc aagcccaagc cgcacaatcc ccacgcctga
     2221 ttgaaatccc tgtccattac ggcggtgaat ttggcgaaga tctgtttgaa gtcgcccaat
     2281 tccacaatac cactcccgaa gagattatcc gccgtcatac cgaaccgact tacaccgtgt
     2341 ttatgatcgg ctttcaaccc ggctttcctt acctcaacgg cttaccgcaa aatttacaca
     2401 ccccacgccg aaacgtgcct cgcaccaaag tccctgccgg ttcggtcggc atcggcggca
     2461 ggcaaacagg catttatccc tttacctcac caggtggctg gcaactgtta ggacgaaccg
     2521 agttaccgtt gtttgatatt caacacaatc cgccaacgct acttacagcc ggcgataatg
     2581 ttagatttgt ggcggagagt ataacgctat gatttatatc gaaataatac agggttttgc
     2641 ccacattcaa gatttagggc gttatggtta tcgccattta ggcgtgagcc acggcggtgc
     2701 gatggacagc cttgccttac aagcgggtaa tttattgtta aaaaatgcaa ataatgcacc
     2761 tgcgattgaa cttacccttg gcggaatgcg gattaccttt gattgcgaca cgccattttg
     2821 cctcaccggt gcattggtgg aggcggagct ggacggcacg ccggtttttt cctattaccg
     2881 ctacactgcc aacgcccgtc aaacgctgac gataaaacgg attgttctcg gcaattacgc
     2941 ctatttatgc gtggcaggcg gttttctggt gccgcaggtg ttaggttcgg caagtaccgc
     3001 tctcaaagct caatttggcg gttttcaagg cagaatgtta aaagccggcg ataacatcgc
     3061 cacaggccgc agagacagcc gactttcttt gatgtcgatt gagccgattg atttcacctc
     3121 acgcattcgg gcaaccgctt cgagcgaata tgaagccttt accgcagata gccgtgaacg
     3181 cttttggcaa caagggtggc aactgcaaaa caacagcaac cgtatgggct accgttttgc
     3241 agaaaaagcg ttggaattga ccgcttccct tgaaatgctc tcctacgcag ccccgagtgg
     3301 tacggtgcaa gtgccgccgg acggccagcc gattgtgctg atggcagatg cccaaaccac
     3361 cggtggttat ccgaaaattg cctgcattat tcaggccgat ctcgggcggt tggcacaaaa
     3421 tccgttcggc tcaacggtgc aatttgagca agtaagccgt gaacaagcgg tcgaaattta
     3481 tcaaaaagat caaaattatt tggaaacaat tcggagaaaa gcgaatgaat cacgttgatt
     3541 taaatgtcga tcttgccgaa ggttgtggca acgatcattc attattacag ctcgttactt
     3601 cggcgaatgt ctgctgtggc ttgcacgccg gtgattacaa tgaaatgcgt aaagcgatta
     3661 actgggcgaa ggaaaacagc gtgcgagttg gcgctcaccc gagttttcca gatagggaaa
     3721 actttgggcg taccaatatg caactgcccg atgatgaact taaagcctgt ctgctctacc
     3781 aactgggagc cgccaaagcc ttatgtgatg cggaaaatat gccgatgagc tacgttaaac
     3841 cgcacggcgc tttatacaat atggccgcca aagaccctca ccttgccacc cttatcgcca
     3901 atacggtcaa acagttcgat cccaacctca aactgatggg cttatccggc agcctgatgt
     3961 tgagcattgc cgagcagcac ggtttggaaa ccatttccga agtctttgcc gaccgccact
     4021 atttagctga cggctcgctc gtgcctcgca cccgtgatga tgcaatggta gaaagcgatg
     4081 atgaagcgat cagccaagtc ttgcaaatgg tgttagacgg cactgtacca accgttgatg
     4141 gtggtaatgt ggcgattaaa gccgacagca tttgcttaca cggtgacggc gaacacgccg
     4201 tggtatttgc cgagcgaatt aaaaaagagt tacaggcaaa ggggattttg gtgaaagcct
     4261 gatcctgttt tagtcaaaat ggacggaaaa ccagaaataa aactcttgga gatcgaactc
     4321 ccctctttag aaaagagggg ctgggggaga tttgacagaa gcgatttacg gagaaaataa
     4381 gtaatttcaa gaggttaaat tgagcattat taactctctc acttcgatga gttctactgc
     4441 caaatctccc ctaacccctc tttgctaaag aggggaatat taccatgata aatccacgaa
     4501 taatgactca ttaacaacaa aaaaggaacc aacacaatgt caaacaacaa ccgtagcgcc
     4561 ctactcgggg cagcattttt aatggcgacc tccgccatcg ggccggggtt cttaacccaa
     4621 acagccacct tcacccaaac cctacttgcc agcttcggct ttgtgatctt actctcgatc
     4681 ttgcttgata tcggtgctca actgaatatt tggcgaattg tagcagtatc ggaaaagaaa
     4741 gctcaagaca tcgccaatgc cgtattgccg ggggcaggtt actttctggc tctgcttatc
     4801 gtgatgggcg gtttagcctt taatatcggc aacgtgggcg gtgcaggttt agggctaaac
     4861 attctcaccg gactttcccc tgaaatgggt gcggtggtca gcggtgcgat tgccgtgggc
     4921 gtgttcctgt tccatgaagc tggtaaagtg atggacagat ttgcacaaat tatgggcttt
     4981 gtgatgattg ctttaaccat cttcgttgcc gtaaaagcca atctgccaat cgacgatgcg
     5041 gtggtgcata cctttgtgcc ggaaaaatta gacgtgattg cgattgtcac gctagtgggc
     5101 ggaactgttg gtggatacat cacctttgct ggtgcgcacc gtttgcttga tgcaggcatt
     5161 aaaggcaaag agaatttaaa agaggtgagc aaaagctcgg tgtcagccat tttaatcgcc
     5221 tcaattatgc gtgtggtact gtttttagcg gtgctgggcg tggtgtcaat gggcgtgcaa
     5281 cttgatccga aaaaccctgc ggctacccca tttgcccacg ttgccggcga tgtcggtttg
     5341 atgatcttcg gcgtggtgat ttgggcagcc tccattacct ctgtgattgg ggcggcttat
     5401 acctcggttt ccttcattac ttcattctcg ccatttatcg aaaaacacaa aaactgtttc
     5461 attgtcgcct ttattgtggt ttcaaccgcc gtgctggcaa ccatcggcag accggcacaa
     5521 gtcttggtgt ttgtagggac gcttaacggc ttaattctac cgatttccct cgggttaatt
     5581 ctgctggcag ctcacaaaac caagattgtg ggcgactaca aacaccctgt gtggatgacc
     5641 gtttcagggg cgattgtggt gattctaatg gcggtgctaa gtgcaatgac cctttcaaaa
     5701 tatatttcgg ggctaatggc ataacgactt agcttcaagc taacctaagg tcaggttttg
     5761 caaattttaa ctactcttgt aaaaaatttg taaaatctga ccgcttgcgt ttaaagtttg
     5821 tgatctacca ctcattttga ggctaaataa tttgaaataa atctgaattt tttctacatt
     5881 ttcttcatca tttctacatt ttaaggataa attatggcac ctgcagaact ctttgcggaa
     5941 ggtataaatt taatggtagc cggaatggga tttgttatgc tgttcctgct ggttttaatt
     6001 tatgccatca gctttatttc caagattatc aatcaatatt tccccgaacc tgtttctatt
     6061 ccagcctcta aaactgtgcc tcaatctccc ccagatgatt tagagcgttt acgccctgtg
     6121 attgccgctg cgattgctca tcatcgtcga attaacgggc aacattaaca agatttcaaa
     6181 aataaaataa ttagaaggaa tttgattatg acaactcaac cgaaaaaaat tgcgattacc
     6241 gatgtggttt tgcgtgatgc acaccaatcc ctttttgcga ctcgtttacg cttagacgat
     6301 atgttaccga ttgcctccga gctggataat atcggctatt ggtcgttaga aacttggggc
     6361 ggggcaactt ttgacagttg tattcgtttt ttaggcgaag atccgtgggt gcgtttacgt
     6421 gagctgaaaa aagcaatgcc gaaaacccca atgcaaatgc tgctacgtgg gcaaaactta
     6481 ttgggttatc gtcattatgc cgacgatgtg gtagataaat ttgtcgagcg ttcggtggca
     6541 aacggtatga gtgtgttccg tgtatttgat gccctcaacg accctcgcaa tatgcaagcc
     6601 gcattacaag cggtcaaaaa acacggcggg cacgcacaag gtactttgag ttacaccacc
     6661 agccctgttc ataccctaca aacgtggttg gatgtaaccg agcaattatt ggaaattggc
     6721 gtggattcgt tggtgatcaa agatatgtcg ggcattttaa ccccaatggc agcctatgaa
     6781 ttagtcagcg aaattaaaaa acgctacgac gttgagctac atctccattg ccattcaacc
     6841 accggtatgg ctgaaatggc gttgctcaaa gcgattgaag ccggtgtcga tggtgtcgat
     6901 acctccattt catcaatgtc cggcacttac ggtcacccgg caaccgagtc tattgtcgcc
     6961 acacttcaag gcacgccgta tgaaaccggc ttgagcattc cagctctgga gaaaatctct
     7021 gcctacttcc gtaacgtgcg taaaaaatat gcaaaatttg aaggacagct ccgtggtata
     7081 gatagccgta ttttggttgc ccaagtgccg ggtggcatgc tgaccaacct cgaaagccaa
     7141 ctcaagcaac aaaatgcgtc ggataaatta gacttggttt tacaagaaat tcctcgtgtg
     7201 cgtgaagatt taggcaatat tccgttggtg acaccaacct cacaaattgt cggcacacaa
     7261 gcggtgatga acgtattaat gggcgaacgt tataaaacta tcgccaaaga aactgcaggt
     7321 attttaaaag gcgaatatgg tcgcacaccg gcaccggtaa acaaagcctt gcaagacaga
     7381 gtgcttgagg gagcagagcc gattaccgac cgcccagccg atcatcttcc gccggaactg
     7441 gaaaaattag tggcagaagt gactgcaagt gcaaaagaaa aaggcattac gctatctgaa
     7501 aacatgattg atgatgtatt gattgtggca ctcttccaac aagtgggctg gaaatatttg
     7561 gaaaaccgca ataatccgtc tgcctttgag ccagtgccaa gtgctgatga agcaaaaccg
     7621 gcacaagcct ccgctgcttc tgcaccaaaa gcccaaacac aatccggttc agccatttat
     7681 accgttgagc ttgaaggcaa agcctttgtg gtgaaggtga gtgaaggtgg cgatataacc
     7741 aatatcgctc caacaccagc aacaccggct ccgcaagcgg tcgattctgc accaaaatct
     7801 gcaaatacgc tatcagccaa tgccgaaccg gtgaatgctc caatggcagg gaacattttg
     7861 aaagtggaag tgaaagaggg gcaacaagtt gctgaaggcg atgtgttact gattcttgaa
     7921 gcaatgaaaa tggaaaccca aatttgtgcg gcaaaatccg gcacgattca aggcattaat
     7981 gtcaaacaag gcgatgttgt ggcggtaaat gatactttaa tgagtattgc ctaggagtcc
     8041 ggtatggaaa gtattattgc actttttaaa ggtatggggc taatgcattt agaactgggg
     8101 caagcgatta tgattgtcat cagtttagtg ttgctttggc ttgccatcgc ccgaaaattc
     8161 gaaccgcttt tactactgcc tattggcttt ggtggtttga tgtcaaacat tccggaagca
     8221 ggtatggcaa tgacggcatt agacaattta ctccacagcg gttctgccga gcaactggca
     8281 attattgctg ctaaggtaaa tagtacaagc cttgatctgc attcgattaa agatgccatc
     8341 gcactggctg ctccctctgt tcaaaatgag cttgaagtaa tggctggcga tatgggctat
     8401 accgccggtg tgttggcatt attctataaa gttgctatcg gctacggcgt tgctccgctt
     8461 attatcttta tgggtgtcgg ggcgatgact gatttcggcc ctttacttgc caaccctcga
     8521 atgttaatgc ttggggctgc ggctcaattt gggatttttg ctaccgtact gggtgcatta
     8581 gggctaaact ggttaggcat tatcgacttc acattgccac aagccgctgc gattgggatt
     8641 atcggcgggg cagatggccc gaccgcgatt tatcttgcca gcaaacttgc ccctgaatta
     8701 ttaggtgcga ttgcggtagc ggcttattct tatatggcgt tagtgccact gattcagccg
     8761 ccgattatga aagccttaac caccgagcaa gaacgtaaaa tccgaatggt tcagctccgt
     8821 aatgtaagca accgtgagaa aattattttc cctatcgtgt tgctattatt agtggcactc
     8881 ttactgccgg atgctgcacc gttattggga atgttctgct ttggcaactt actgcgaacc
     8941 agtggcgtag tagaacgctt aaacgacacc gcccaaaatg cgttaatcaa tatcgtgacg
     9001 attttcttag ggttatcggt gggggcaaaa ttggtcgcag acaaattctt acaaccgcaa
     9061 acactaggca ttttagtatt gggcgtgatt gcctttggga tcggcactgc aagcggtgtg
     9121 attatggcga aaataatgaa ccgctttagt cagcacaaaa tcaacccact tatcggctca
     9181 gcgggtgttt ctgccgtgcc gatggctgca cgggtatcaa acaaagtcgg acaagagtac
     9241 gataaacaaa acttcttgtt aatgcacgca atgggcccaa acgttgccgg cgtgattggc
     9301 tctgcgattg cagcgggggt gatgttgaaa tacatttcaa cgatgtaaat cctcatttgc
     9361 aaaaaacctc cgaaaaataa ccgctctcgg aggttttttc taaaaatacc gcttaccaac
     9421 actcttacaa tacgaaaatt ccgctgtaca ttcttcacta attgccgtag tatccatctt
     9481 a
//
LOCUS       DANLYI010000085         9401 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.085, whole genome shotgun sequence.
ACCESSION   DANLYI010000085 DANLYI010000000
VERSION     DANLYI010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 9401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9401
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.085"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            <1..2059
                     /gene="glyS"
                     /locus_tag="PXD81_001444"
     CDS             <1..2059
                     /gene="glyS"
                     /locus_tag="PXD81_001444"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005693270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HDL5537464.1"
                     /translation="QNFLAEIGTEELPPKALKKLATAFAENVENELNQAGLSFEKVEW
                     FAAPRRLAVKVLSLAESQPSKEVEKRGPAVSAAFDAEGKPTKAAEGWAKGCGITVEQA
                     ERIATDKGEWLVHRAVIEGQPTKNLLVDIIAKSLANLPIPKTMRWGDKTEQFVRPVHT
                     VTLLFGAELIEGEILGVKSGRTIRGHRFLGEREFQIENADQYPQLLKDRGSVVADFNE
                     RKALILAKSQEKATALGGVADIEEDLLDEVTSLVEYPNVLTAKFEERFLAVPAEALVY
                     TMKGDQKYFPIYSKDGKLLPHFIFVSNINPEDPSKIIEGNEKVVRPRLTDAEFFFKTD
                     LKQKLEDQLPRLETVLFQQQLGTLRDKTARIEALAGEIAKQIGADETKAKRAGLLSKC
                     DLMTNMVFEFTDTQGVMGMHYARHDGEDEEVAVALNEQYMPRFAGDNLPNSLVACSVA
                     LADKFDTLTGIFGIGQAPKGSADPFALRRAALGSLRIIVEKNLPLDLTDLVAKSAQLF
                     GDKLTNKDVVEDVVDFMLGRFRAWYESEGIAVDVIQAVLARRPTKPADFDARVRAVSH
                     FRTLDSAEALAAANKRVSNILAKADIAIGDIDVSACVEPAEKALAEAVIALKAEVQPL
                     IAQGDYTAVLDKLANLRQPVDAFFDGVMVNAEDQKLRQNRLAILSTLQGLFLQVADIS
                     LLQ"
     gene            complement(2164..3465)
                     /locus_tag="PXD81_001445"
     CDS             complement(2164..3465)
                     /locus_tag="PXD81_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine dehydratase subunit alpha family protein"
                     /protein_id="HDL5537465.1"
                     /translation="MNIHCEKLVQSILHAVNHEVVPALGCTEPISLALASAIARQYLG
                     HLPERIEAKVSPNLMKNGMGVAVPGTATVGLPMAAAIGAIGGEPDGGLEVLKNITPEQ
                     VKQAKAMLNTDKVSVSIFETEHILYSEATLFHQANRVCVRIAEHHTNVIYIEKNGQTI
                     LSKPCAVNNDNTTEIFASLSAKDIFNFSMQIELEKIRFIEQAAQLNSALSQEGLRADY
                     GLHIGRTLQKQIGLGLISDDLLNRIVIETTSASDARMGGASLPAMSNSGSGNQGITAT
                     MPIVVVARHLNVTDEQRIRALFLSHLMAIYIHSKLPKLSALCAVTTAAMGSCAGIAWL
                     LTGKFENISMAISSMIGDISGIICDGASNSCAMKVSTSVTSSYKSILMSMDNSQVTGN
                     EGIVEHDIDRSINNLCSIASRSMQYTDRQVIEIMVNKPKLK"
     gene            complement(3479..4756)
                     /locus_tag="PXD81_001446"
     CDS             complement(3479..4756)
                     /locus_tag="PXD81_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5537466.1"
                     /translation="MDTQKLSPAEFKQATKFDSTDFGWIIMSIGMAIGAGIVFLPVQV
                     GLMGMWVFLLSSIIGYPAMYLFQRLFINTLAESPECKDYPTVISGYLGKNWGIFLGVL
                     YFIMLVIWMFVYSTAITNDSASYLLTFGITNTLLSDNPFYGLVLICILVALSSRGEKL
                     LFKISTFMVLTKLFVVAALGLAMIGMWQLHNIGAMPPFGKLIKDAIITLPFTLTSILF
                     LQTLSPMVISYRAKEQSREVARYKALRAMNIAFAILFITVFFYAVSFTLAMGREEALK
                     AYEQNVSALAIAAQFFPGSWATYVGVALNIFAVMTAFFGVYLGFREATQGIVMNLLLR
                     KFPAEKINPNYVQKGIMLFSILLAWSAIVLNAPVLSFTSICSPIFGLVGCLIPAYLVY
                     KVPALFKYKGLALNIIIITGILLCISPFLTFLE"
     gene            complement(4785..5453)
                     /locus_tag="PXD81_001447"
     CDS             complement(4785..5453)
                     /locus_tag="PXD81_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HDL5537467.1"
                     /translation="MPDKLEYLHDLDKEIMLSYFSLSEAIAGLLGEFCEVVIHSLNAY
                     EKSVIKIINGHHTNRKIGSPITDKGLQLLKCYQKDQSNTELVYFSRLNNGNLVKSTTH
                     IIIGHEGKPIGLFCINLNLSTPLDKTVSSFMNYDPIPPHYKSENFITHPKELIEETFL
                     KAVTHIEQHTTKHSKLYKKSIIFYLFENGIFELKEAVPIVAEKLSLTSHAIYKHLREF
                     KNNT"
     gene            5687..5977
                     /locus_tag="PXD81_001448"
     CDS             5687..5977
                     /locus_tag="PXD81_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5377 domain-containing protein"
                     /protein_id="HDL5537468.1"
                     /translation="MSVKTEVLFSNHWNVRVSDPGFEGSPSHYFETVYLTLEAFINGE
                     NVHYEFTRKVEQEVKTHRSFTDVNELFEFLGEYLSPVALGNVGVRIGQLGLA"
     gene            6044..6955
                     /gene="xerC"
                     /locus_tag="PXD81_001449"
     CDS             6044..6955
                     /gene="xerC"
                     /locus_tag="PXD81_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HDL5537469.1"
                     /translation="MTTSNPLFQQTRPYWDFLRIEKQVSPHTLSNYQRQLMAVSELFS
                     QAGIENWEEVESSSVRWMLSQSHKQGLSAKSIGLRLVALRQWFVYLIKIGRMSANPTL
                     GVKSPKVGKHLPKNIDAEQVGQLLAIESDEPSDIRDIAMMELMYSSGLRLSELQGLDL
                     GDMDLSAREVRVQGKGSKERIVPIGSQALKALNRWLEMRLQFKPQDNAVFLNKRGGRL
                     SHRSIQLAMKKWGERQGLESHLHPHKLRHSFATHMLEASGDLRAVQELLGHSSLSTTQ
                     IYTHLDFQHLAKIYDSAHPRARRKQED"
     gene            complement(6952..7323)
                     /locus_tag="PXD81_001450"
     CDS             complement(6952..7323)
                     /locus_tag="PXD81_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005602656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HDL5537470.1"
                     /translation="MKYLYIICGFICIGLGIIGAVLPGLPTTPFLLLALFCFTKGSER
                     IRQWFIQTKLYKKYLQEYDEKRAMTMKQKITILAISFPFTLFAFFALPHIGGKIALVV
                     LVIIQYWYFFCKMKTLEPENA"
     gene            complement(7479..7568)
                     /locus_tag="PXD81_001451"
     tRNA            complement(7479..7568)
                     /locus_tag="PXD81_001451"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7532..7534),aa:Ser,seq:tga)
     gene            complement(7660..8751)
                     /gene="trmA"
                     /locus_tag="PXD81_001452"
     CDS             complement(7660..8751)
                     /gene="trmA"
                     /locus_tag="PXD81_001452"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011848685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HDL5537471.1"
                     /translation="MTLPIEQYSDLLAKKTENLTALLKPFNPPAIEVFASKPSHFRMR
                     AEFRVWHEEGELYHIMFNPETKARYRVDSFPIASELINHMMTALLAEIKGNELLTRKL
                     FQIDYLSTLSGEIAVSMLYHKSLNEEWVEQANALKSRLEQQGFKVQIIGRANKQKIAL
                     DCDYVEEILPVHGKNYVYRQVENSFTQPNAEMNIKMLEWARNCTKNSEGDLLELYCGN
                     GNFSIALAENFRKVLATEISKSSVQSAQYNIEKNGIVNLQIIRMSAEEFTQAMNGVRA
                     FYRLKGIDLNAYECNTIFVDPPRAGLDQATLNMVQAYERILYISCNPETLAENLQQLS
                     QTHRIEKAALFDQFPYTHHIESGVWLIRK"
     gene            complement(8816..9148)
                     /locus_tag="PXD81_001453"
     CDS             complement(8816..9148)
                     /locus_tag="PXD81_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018650843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF413 domain-containing protein"
                     /protein_id="HDL5537472.1"
                     /translation="MADSFSVTRRFFDDKNYPRGFSRHGDYTIRESQTLEQFGQACLA
                     LESGERKPATQEEKRFVAVMKGEEVAETDIEKAWLKYRTLTSKTKRIYTLSGNAGSDA
                     GDDFSAAE"
BASE COUNT         2765 a         1847 c         2091 g         2698 t
ORIGIN      
        1 acaaaacttc cttgccgaga tcggcacaga agagctgcca ccgaaggcac tcaaaaaatt
       61 agcgaccgca tttgcggaga atgtagaaaa cgaattaaac caagcaggtc tttcgtttga
      121 gaaagtggaa tggtttgctg ctcctcgccg tttggcggtg aaggtgttga gccttgcgga
      181 aagccagccg agtaaagagg ttgagaaacg agggccggcg gtgtcggcag cgtttgatgc
      241 cgaaggcaag ccgactaagg cagcggaagg ctgggcgaag ggctgcggga ttaccgtaga
      301 gcaagctgag cgtattgcca ccgacaaagg cgaatggttg gtgcatcgtg ccgtgattga
      361 aggtcagccg actaaaaatc tgttggtcga tattatcgcc aaatcgctgg cgaacttgcc
      421 gattccaaaa acgatgcgtt ggggcgataa aaccgagcag ttcgtccgcc ctgtgcatac
      481 cgtgacactt ttattcgggg cggagttgat cgaaggtgag atcttaggcg tgaaaagcgg
      541 ccgtactatt cgtggtcacc gtttcttagg tgaacgtgag ttccaaattg agaacgccga
      601 tcaatatccg caactgttga aagatcgtgg atctgtggta gcagatttca atgagcgtaa
      661 agccttaatt cttgcaaaat cacaggaaaa agcaaccgct ttgggtggcg ttgccgatat
      721 tgaagaagat ttgctggacg aagtgacctc gttggtggaa tacccgaacg tactcaccgc
      781 caagtttgaa gagcgtttcc ttgctgtgcc ggcggaagcc ttagtttaca ccatgaaagg
      841 cgaccaaaaa tacttcccga tttattcaaa agacggcaaa ttgttaccgc actttatttt
      901 tgtgtcgaac atcaatccgg aagacccaag caaaatcatc gagggtaacg aaaaagtggt
      961 tcgcccacgt ttgaccgatg cggagttctt cttcaaaacc gacttaaaac agaagttgga
     1021 agaccaactg ccacgccttg aaaccgtgtt gttccagcaa cagcttggca cattgcgtga
     1081 taaaaccgct cgcattgaag cgttggcagg tgaaattgcg aagcaaatcg gggcagatga
     1141 aaccaaagcg aaacgtgcag gtctcctctc aaaatgtgac ctgatgacca atatggtgtt
     1201 tgaatttact gatacccaag gcgtgatggg tatgcactat gcccgccacg acggtgaaga
     1261 tgaagaagtg gcggtggcgt taaacgagca gtatatgcca cgttttgctg gcgataactt
     1321 gccaaattca ttggtggctt gctcggtggc gttggcggac aaatttgaca cgctaacagg
     1381 gattttcggc attggtcaag ccccgaaagg cagtgccgac ccgttcgccc tacgccgtgc
     1441 ggcacttggc tcgttgcgga ttattgtgga gaaaaattta ccgcttgatt taaccgattt
     1501 agtggcgaaa tctgctcaat tatttggcga taaactcacc aacaaagacg tggttgagga
     1561 cgtggtggac tttatgctcg gccgcttccg tgcgtggtat gagagcgaag gcattgcggt
     1621 ggacgtgatc caagccgttc tcgcccgccg tccgaccaag cctgccgact tcgatgcccg
     1681 tgtgcgtgcc gtgtctcact tccgcaccct tgacagtgcc gaagccttag ccgcagcgaa
     1741 taaacgggtg agtaacattc ttgccaaagc cgatattgca attggcgaca ttgatgtttc
     1801 agcttgcgta gagccagcag aaaaagcatt agccgaggcg gttattgcgt tgaaagccga
     1861 agttcaaccg ctgattgcac agggcgacta caccgctgtg ctggataaat tagcgaacct
     1921 ccgccaaccg gttgatgcct tctttgacgg agtaatggta aatgcagaag accaaaaact
     1981 gcgtcaaaac cgcctcgcca ttttaagcac gctacaaggc ttgttcctgc aagtggcgga
     2041 tatttcgctg ttgcaataac aataagtttg caataattgc aaaattttaa tgaaaataga
     2101 ccgcttgcag taattgtgat ctgcaagcgg tcttttttgc ttgaaatttt accaaaggcg
     2161 ggattattta agttttggtt tattcaccat aatctcgatg acttggcggt ctgtgtattg
     2221 catactacga gaggcaatgc tgcataagtt attaattgaa cgatcaatat cgtgttcgac
     2281 aattccttcg ttgcctgtta cttggctatt atccatagac atcagaatgg atttatagct
     2341 tgatgttacg ctggtggaga ctttcatcgc acaactgttt gaagcaccat cacagataat
     2401 accgctaata tcgccgatca tactactaat tgccatactg atattctcaa atttccctgt
     2461 gagcagccac gctatgccgg cacagcttcc catagccgcg gttgttacgg cacagagtgc
     2521 agataatttc ggtaacttgc tgtgaatata aattgccatg aggtgtgata aaaagagtgc
     2581 tctaatgcgt tgctcatcag tgacatttaa atggcgggca acgacaacaa tcggcatagt
     2641 tgctgtaatt ccttgattac ctgaacccga attgctcata gcaggtaaac ttgcaccgcc
     2701 cattctagca tcagaggcag aggtggtttc tataacaatg cgattaagca aatcatcact
     2761 gattagacct agaccaatct gtttttgcaa tgtccttccg atatgtagcc cataatcggc
     2821 acgtaatcct tcttgtgaga gtgcggaatt taattgtgct gcttgctcaa taaagcgaat
     2881 tttttccaat tcgatttgca tagaaaaatt aaatatatct tttgccgaaa gtgaagcaaa
     2941 gatctcagta gtattatcgt tattgacggc acaaggtttg cttaaaatag tttgcccgtt
     3001 tttttcaatg taaatgacat tggtatggtg ttcagcaata cgaacacata ctcgattcgc
     3061 ttggtgaaat agtgttgctt cagaatatag aatatgttct gtttcgaaaa tagagacaga
     3121 cactttatcg gtatttaaca tagctttagc ttgcttaacc tgttctggcg taatattttt
     3181 taatacttct aatccaccat caggctcccc tccgattgct ccaatagctg ccgccatagg
     3241 caatccgacg gttgcagttc ccggtactgc tactcccatc ccatttttca tcaagttagg
     3301 ggaaactttt gcctcaatac gttctggtaa atgacctaaa tattggcgtg caatggcgga
     3361 tgcaagggcg agagaaatag gttctgtaca gcctaatgca gggacaactt catggttgac
     3421 tgcatgtaaa atggattgta ctaatttttc gcagtgaata ttcatagata aaaccttatt
     3481 actctaaaaa tgtgaggaat ggagaaatac ataataaaat gccggtaatg ataataatat
     3541 tgagtgctag ccctttatat ttgaataatg caggcacttt atagactaaa taggccggaa
     3601 tcaagcaccc aactaagcca aaaataggac tacagattga ggtaaagctt aaaaccggtg
     3661 catttaatac aatcgcactc caagccagca gaatggaaaa taacataatc cctttctgca
     3721 cataattagg attgattttt tcagccggga attttcttaa taataggttc atcacaatgc
     3781 cttgagtggc ttctctaaaa cctaaatata ccccgaaaaa ggctgtcata acggcaaaaa
     3841 tatttaatgc aacacctaca taggttgccc aactaccagg gaaaaattgg gctgcaattg
     3901 ctaatgcgga aacattttgc tcgtatgctt tgagcgcttc ttctctcccc attgcaagtg
     3961 taaaagagac agcgtaaaaa aaaacggtaa taaataagat agcaaatgca atattcatcg
     4021 ctcgtagggc tttatagcgc gcaacttcac gagactgttc tttagcacgg taagaaatta
     4081 ccattgggct gagagtttgc aagaagagta tagaggttag ggtgaaagga agggtaatga
     4141 tggcatcttt tataagtttg ccgaaaggag gcattgcacc gatattatgg agttgccaca
     4201 ttccaatcat tgcaagccct aaggcggcaa cgacaaatag cttagttaat accataaatg
     4261 tagatatttt aaacagtagc ttttcgcctc gagaagataa ggcaactaat atacagatta
     4321 gcactaagcc ataaaatgga ttatcagaga gtagtgtatt ggtaatgccg aaggtaagaa
     4381 gataggaggc actgtcgttt gtaattgcgg tagaataaac aaacatccaa atcactaaca
     4441 taataaaata aagtacacct aaaaagattc cccagttttt gcccaggtag ccactaataa
     4501 cggtggggta atctttgcat tccggagatt ctgctaaagt attaatgaac agacgctgaa
     4561 aaaggtacat cgccgggtat ccgataatag aagaaagtag aaaaacccac atgcccataa
     4621 gtcctacttg taccggtaaa aagacgatgc ctgccccgat agccatacca atactcataa
     4681 tgatccagcc gaagtcggta gaatcaaatt ttgttgcttg tttaaattcg gcaggcgaga
     4741 gtttttgagt atccatagag tctccttaaa tcgtaacagt ataattaagt gttatttttg
     4801 aattctctta aatgtttata aattgcgtgg ctggttaggg agagtttttc tgccacaata
     4861 ggtactgcct cttttagctc aaagatgcca ttttcaaaca aatagaagat aatggatttc
     4921 ttatagagtt ttgaatgttt ggtagtgtgt tgctcaatgt gagtaactgc ttttagaaag
     4981 gtttcttcaa taagttcttt tgggtgagta ataaagtttt cacttttata atgaggcgga
     5041 ataggatcgt aattcataaa agagctaacg gttttatcta atggtgtcga taaatttaaa
     5101 ttaatacaaa ataatccgat aggtttgccc tcatgcccga taataatatg cgtggtggac
     5161 ttaactaagt taccgttatt taaccgtgaa aagtaaacca gctcagtatt agattgatct
     5221 ttttggtaac attttagtaa ctgtaatcct ttatctgtaa taggcgaacc tatttttcgg
     5281 ttagtatgat gtccgttaat gattttaata acggattttt cataggcatt caatgaatga
     5341 ataaccacct cacagaattc tcctaataac cctgctattg cttcacttaa tgagaaataa
     5401 gacagcataa tttctttgtc taaatcatga agatactcta atttatctgg cataactttt
     5461 actccaattt ttgtgaatta taagaatgag taaattttaa agtcaattat atttaacttt
     5521 attaagagtt aaaagtcagt attttttgac caagatcata ttttgaggga aagatataag
     5581 aggacaaacc aaataagatt tgcctcgatt ctgactagga aaagtggcaa attcaatcta
     5641 tcttgattaa aataatgtac gtacttatag gaagaaggaa aacacaatgt cagtaaaaac
     5701 cgaagtttta tttagtaatc attggaatgt tcgagtgagt gatccgggct ttgaaggctc
     5761 gccaagccac tattttgaaa ccgtatattt aacgctggaa gcctttatta acggcgaaaa
     5821 tgtacattat gaatttactc gtaaagtgga gcaagaagtg aaaactcatc gcagttttac
     5881 tgatgtgaat gaattgtttg aatttctcgg cgaatattta agccctgttg cacttggtaa
     5941 tgttggcgtg cgtatcggtc agttggggct agcctaatct caaagggcaa atcttttgcc
     6001 cttgcaacat tttcctaaaa attaaccgct tgttattacg tttatgacca cttccaaccc
     6061 tcttttccaa caaacccgcc cctattggga tttcttacgc attgaaaagc aagttagccc
     6121 gcatacctta agcaattatc aacgccaatt aatggcggtg agcgagcttt tttcgcaagc
     6181 aggcattgaa aattgggaag aggtcgaaag tagctcggtg cgttggatgt taagccaaag
     6241 ccataagcaa gggttgagtg cgaaaagtat cggcttacgc ttggtggcgt tacggcaatg
     6301 gtttgtctat ttgattaaaa tcgggcgaat gtcggcaaac ccgactttag gggtgaaatc
     6361 gccgaaagta ggtaagcatt tgccgaaaaa tattgatgcg gagcaggtcg ggcagttgct
     6421 ggcgattgag agtgatgagc cgagcgatat tcgagatatt gcgatgatgg agctgatgta
     6481 tagttccggc ttgcgtttat cggaattaca agggctggat ttaggcgata tggatttatc
     6541 ggcacgagaa gtacgggtgc aaggtaaggg tagcaaagag cgaattgtgc cgattggctc
     6601 gcaagcgtta aaagcgttaa atcgctggtt agaaatgcgt ttgcaattta agccgcaaga
     6661 taatgcggtg tttctgaata agcgaggtgg gcggttatct catcgttcca ttcagttggc
     6721 aatgaagaaa tggggtgagc gacaagggct ggaaagccat ctgcatccac ataaattacg
     6781 tcactctttt gcgactcata tgttagaggc aagcggtgat ttgcgagcgg ttcaggaatt
     6841 attgggtcat agtagcctgt ccaccactca aatttatacg catctggatt tccaacactt
     6901 agctaaaatt tatgactctg cccacccaag agcaaggcgg aagcaggaag attaggcatt
     6961 ctccggttca agggttttca ttttgcagaa gaaataccaa tattgaatta tcactaacac
     7021 tactaacgca attttcccac cgatatgggg cagtgcaaag aaagcaaata gtgtaaacgg
     7081 aaacgaaatc gctaaaatcg tgattttttg cttcatcgtc attgcccgtt tctcatcata
     7141 ttcttgcaga tattttttgt ataatttggt ttgaataaac cactggcgga ttctctccga
     7201 gcctttagta aagcagaaca gagccaaaag taagaacggt gtggtcggca agcccggtaa
     7261 tactgccccg atgatgccta aaccaataca aataaaacca cagataatat aaaggtattt
     7321 cataataatt aagttattga taatgaatcg caattatcct aaagcaactt taggctgatt
     7381 tcaaccagtt tatagcgatg gattcaagcg gtggttttat ctgattttct tgcaaatgaa
     7441 ataaaaaaac ctgctgattg ctcaacaggt ttaaagactg gcggaggaag tgagattcga
     7501 actcacggag ggcgttaacc ctcgccggtt ttcaagaccg gtgcattcaa ccgctctgcc
     7561 attcctccgt tgaaaacgtt gcaataatag agattattgc gaacatcgtc aagtaataaa
     7621 tcttaaaagc tgcttattcg ttcttttttt cagcacaatt tattttctaa ttaaccaaac
     7681 gccactttca atatggtggg tgtaagggaa ttggtcaaaa agagcggctt tttcaatccg
     7741 atgggtttgg cttaattgtt gtaaattctc ggcaagggtt tccggattgc aagagatata
     7801 gagaatccgc tcatacgctt gcaccatatt taatgtagct tgatctaacc ctgcacgagg
     7861 tggatcaaca aaaatggtat tgcattcgta ggcgtttaaa tcaatccctt ttaagcgata
     7921 aaaggctcgc acaccgttca tcgcttgggt aaattcttct gccgacattc ggataatttg
     7981 taggttaaca atgccatttt tttcaatatt gtattgagcc gattgcactg aagattttga
     8041 gatctcggtt gccagcactt tgcggaagtt ttccgccaag gcaatcgaaa aattgccgtt
     8101 gccgcagtaa agctcaagca aatcgccttc actatttttt gtgcaatttc tcgcccattc
     8161 cagcatttta atattcattt ccgcattcgg ctgggtaaag ctgttttcca cttggcgata
     8221 cacataattt ttgccgtgta ccggtagaat ttcttccaca taatcgcaat ccagtgcgat
     8281 tttttgttta ttggcacgcc cgataatttg cactttgaaa ccttgttgct ctaaacggct
     8341 ttttaaggcg tttgcctgtt caacccattc ttcattcagg cttttgtggt aaagcattga
     8401 gactgcaatt tcaccgctta aggtgcttaa ataatcgatc tggaacagct tgcgagtcag
     8461 tagttcattg cctttaattt ccgccaataa tgcggtcatc atatggttaa tcagctcact
     8521 ggcaataggg aagctatcca cacgataacg agctttggtt tcagggttga acataatgtg
     8581 gtacagctcg ccttcctcgt gccacactcg aaattctgcc cgcatacgga aatggcttgg
     8641 ctttgaggca aatacctcaa tcgcaggcgg gttaaacggc ttgagtaaag cggtcaaatt
     8701 ttcggttttt tttgcaagaa gatcggaata ttgttcgata ggaagagtca ttttttacca
     8761 caaatagcaa atgaaaaaac aaaccacgaa ttacttcgtg gttttaaatt agaaattatt
     8821 cagcagcact gaagtcatcg ccggcatcgc tacctgcatt gcctgaaagg gtgtagatgc
     8881 gttttgtttt actagttaat gtgcggtatt ttaaccacgc tttttcaatg tcagtttctg
     8941 ctacctcttc gcctttcatt accgcaacga aacgtttttc ttcttgagtt gccggcttac
     9001 gttcaccact ttctaaagcc aagcaagctt gaccgaattg ttctaaagtt tgtgattcac
     9061 gaatggtgta atcgccgtgg cgagagaaac cacgtggata atttttatcg tcaaaaaagc
     9121 ggcgcgtaac gctgaaactg tctgccataa taatgcctcg ttgtttgaaa ttagggaaaa
     9181 taaaatcggc atacattaaa caaaaacagc gaataaatca atgccttaat gtaaaaaagc
     9241 ggtcaaattt ggcaaatttt ttgcaaaaaa ctctttaagg agaaatattg atccttttaa
     9301 aatagacaat gcagtctgag gtatattttt tatatttgtt aaggtatatt acctaaatgt
     9361 attgagtttt gattaacttt aatatttgga gatatactgt g
//
LOCUS       DANLYI010000086         9373 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.086, whole genome shotgun sequence.
ACCESSION   DANLYI010000086 DANLYI010000000
VERSION     DANLYI010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 9373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9373
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.086"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            177..554
                     /locus_tag="PXD81_001454"
     CDS             177..554
                     /locus_tag="PXD81_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009875251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacL family protein"
                     /protein_id="HDL5537473.1"
                     /translation="MEYQFTHSIHGVVAKCSMDHEAFARWLNAEITENPSQLAPIFAE
                     IEKCRAAFPNHYECVFEGREYSLYFDYDEVMAKANNLDAAFDDEEMEEGFQFYNEESI
                     AFCGLDDFEKFLKAYQHFVQTYH"
     gene            643..1230
                     /gene="yihI"
                     /locus_tag="PXD81_001455"
     CDS             643..1230
                     /gene="yihI"
                     /locus_tag="PXD81_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005608169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HDL5537474.1"
                     /translation="MSRTKKTRRITDIMPARKTDKPKQPMPKLGGGKNRKLTRYELDA
                     QAREEKRKRKHKGLPTGSRNADPAEQKKAVVKEVKDPRIGSRKKVPLMVEFVNQPEKG
                     RTIKAVPVEPIKPTLSPEQELEQLENNECLNQLLDDLEAGKTLSAEDQKFMNECLDRI
                     DELMTELGIEYEDEEGDNGDALLRQFETMDINKFR"
     gene            1244..1711
                     /locus_tag="PXD81_001456"
     CDS             1244..1711
                     /locus_tag="PXD81_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012263040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2489 domain-containing protein"
                     /protein_id="HDL5537475.1"
                     /translation="MLRIFLMVLAALILISLGGYALHLMLKVRAQKKHEQALIEQAKQ
                     AQKDRYIRILDSIEVIARAMISEQCDFSEGVLRLKPLLDVLGRKLSDYTAMWALYGVV
                     ENMPILDERKNLKRNERMKLDLEREAKEAELESDIKNECYQLLKDIEEMRKAI"
     gene            1711..3078
                     /gene="hemN"
                     /locus_tag="PXD81_001457"
     CDS             1711..3078
                     /gene="hemN"
                     /locus_tag="PXD81_001457"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HDL5537476.1"
                     /translation="MSEIIWDLALIQKYNHSGPRYTSYPTALEFNENYTNEDFIRAAA
                     RYPERPLSLYVHIPFCHKLCYFCGCNKVITRHRHKVEIYLDYLEREIKTRAPLFQNRL
                     VTQIHWGGGTPTYLDEDQSARLMQMLRANFDVSDDAEISIEMDPREIELSMLDHLRAI
                     GFNRLSMGIQDFDKEVQRLVNREQDEEFIFALMKRAKELGFVSTNVDLIYGLPKQNVE
                     SFMYTLKRVVELNPDRMSVFNYAHLPSRFAAQVKIKEELLPAPETKLTILQNSIEFLG
                     KNGYKFIGMDHFAKPDDELAIAQEKGILHRNFQGYTTQEECDLLGMGVSAISLLGDTY
                     AQNRKELQQYYADVETNGIALHKGIALTQDDCIRRDVIKALICNFKLDFATIEKQYGI
                     DFKRYFAEDLAFLAPLAEDELLTMNDDSIVVSPRGRLLIRNICLCFDVYSRQLARRQQ
                     FSRII"
     gene            3152..5485
                     /gene="lptD"
                     /locus_tag="PXD81_001458"
     CDS             3152..5485
                     /gene="lptD"
                     /locus_tag="PXD81_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005617440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HDL5537477.1"
                     /translation="MKQRYSLLSLAVMAACYSNSAAADLKQQCLLGVPHFQGEVVEGD
                     QLRMPVTIEADSAVINQPRDATYTGDVTIKQGNRSIWADQVRVEQDGENARMAYLQGN
                     FNYQDNLIQATGSDATINLLSKDANLANANYQLIGRQGRGTAESGEFGNSKHTLKNAS
                     FTSCLPNDNSWQIEANEMIQHVDEEYAEMWHARFKVLGMPVFYSPYLQFPIGDRRRSG
                     LLIPSFERSSKNGWTYSQPFYWNIAPNMDSTITPTYYSRRGWQISPEFRYLTKIGQGL
                     VATEYMAKDRLSEYQPGNDDRKRYLFHWRHAMSFLTDWRFSVDYTKVSDRRYFSDFDS
                     DYGSSTDGYATQNFKLGYYQPNYNISISGKKFQTFDELDIGPYRVLPQIDFNYYKNDL
                     IKGGDFKFFAQAARFENDSKLMPKAWRFHAEPTLNFPLANRYGSLNLETKLYATYYQQ
                     TKGSESTEEIKKSVSRVLPQIKLDFKTVLESDKQLFSGYTQTLEPRVQYIYRPYKDQS
                     DIGSKAHRSVSLGYDSALLQNDYFSLFNDRRYSGLDRISSANLVTAGGTTRFFKEATG
                     EEVFNFSLGQTYYISPSKIDDTSDNSTKGHTTSWAAESNWKFHKNWNWHGSYQYDTRL
                     NQTALANMSLQYKPQSDKLIQLNYRYASKDYINQNLNTNKYGQSIKQVGGVLGWELTD
                     KVSVMTSYYHDLALKKPVEGQISLNYNTCCWAANVYVARRLVSTPVGSSDTINDFYYD
                     NKFGINFELRFGTNYSNGVTRMLKRGIIPYTEPYSIN"
     gene            complement(5627..8266)
                     /gene="ppc"
                     /locus_tag="PXD81_001459"
     CDS             complement(5627..8266)
                     /gene="ppc"
                     /locus_tag="PXD81_001459"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011609148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HDL5537478.1"
                     /translation="MNQQYSTIQHNIHMLGEFLGETIRDAQGNYILELIESIRVLSRN
                     SRAGDENAREQLLDKLANISNDDVLPVARAFSHFLNLTNIAEQHQTVSRHHIDESLGS
                     RSLDSLFLRLKSQNIPVEKVIKTVESLMIDLVLTAHPTEVTRRSLGHKHVEINKCLNL
                     LEHDDLTEAESYKIKRRLMQLIALAWHTNEIRTQRPTPVDEAKGGMAVIENSLWKAVP
                     EFCRQLNFYLEKHFGVQYPVNLAPVRFSSWMGGDRDGNPFVTAETTRRVLRMNRWKAT
                     ELFLQDIKNLAEELSITQCTPAFRAKYGNHLEPYRVLLKVLRQKLINTEIYYAELLEG
                     KISTIDESDILTSDQQLWEPLFDCYESLQACGMRIIANGALLDCLRRINCFGLGLSRL
                     DIRQESTRHEMAIAEITRYIGLGDYAHWTEDDKQAFLVRELSSRRPLIPTNWTPSPET
                     KEVLDTCKIVAEQPEGVISAYVISMAREASDVLSVHLLLKEAGCKFTIPVAPLFETLG
                     DLDHSEKVMTDLFNIGWYRGVINNYQMIMIGYSDSAKDAGMLAAGWAQYRAQEALVNL
                     CEKYNIELTLFHGRGGTIGRGGAPAHAALLSQPPRSLKNGLRVTEQGEMIRFKLGLPA
                     VAVESLELYASAILEANIFPPPEPKQQWRDVLAKASDISCQIYRDMVRGELDFVPYFR
                     SATPEQELSRLPLGSRPAKRNPKGGVESLRAIPWIFAWMQNRLMLPAWLGAGQALQQL
                     IDEGNETLLKEMCNEWPFFSTRIGMLEMVFSKADIWLAEYYDQRLVEPKLHRLGNMLR
                     TQLQADLKTVLALAHDGQLMADLPWIAESIALRNIYTDPLNLLQVELLDRIRKQGENA
                     DPVLEQALMITITGIAAGMRNTG"
     gene            8391..9359
                     /locus_tag="PXD81_001460"
     CDS             8391..9359
                     /locus_tag="PXD81_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS1 family transporter"
                     /protein_id="HDL5537479.1"
                     /translation="MCLKLLFGFDNLPVVYVLFTVIQVGLSINGFKGIKWLENVSCVF
                     LIGILAYMLYVVNTKFSVEIGERFANIEGTWGMPFWAATTAFLGIYSTMILNASDYSR
                     HVKKGTTPTATGVIYVLSILPVTLFMGLIGLLVTAATGNSDPIEVFATAMDNKFLTVI
                     TLLFIAFAQVTTNVLNNIVPPSYILMDSFKMKWSHATILVGILSICVMPWKLVTAESA
                     DGLSLFIKIYSAFLGPIFAVMVVDYYILRKRTLNVNDLYNKEGIFKGINWAAIIAIAI
                     GSFCSLIVVDLSWYVSLIPSGLSYYILMRVLKSSEAFRKGTVLEHQ"
BASE COUNT         2835 a         1772 c         2032 g         2734 t
ORIGIN      
        1 gtagttcctt tttgatgtat caatactaaa aataacctgt attattctag cagatttctg
       61 ggtaatcgag gagttgataa catttaaaaa ttagcgtttt gcaaaatttt ccaaaaaaag
      121 accgcttgtt aggctataat agccaaccaa acagaccaat tcaaaggaga atcggtatgg
      181 aatatcaatt tacccatagt attcacgggg tggtggcaaa atgttcgatg gatcacgagg
      241 cgtttgctcg ttggttgaat gccgaaataa cggaaaatcc tagccagtta gcccctattt
      301 tcgctgaaat agagaagtgt cgagccgcat ttccgaatca ttatgaatgt gtgtttgaag
      361 ggcgggagta tagtctctat tttgattatg atgaagtgat ggcaaaagca aacaatttag
      421 atgctgcttt tgatgatgag gaaatggaag aaggctttca attttacaat gaagagagca
      481 tcgcattttg tggcttagat gactttgaga aatttttgaa agcctaccaa cattttgtgc
      541 aaacttatca ttgattgaaa tgattaattg aaaccaaagc tattaaggga atctaattcc
      601 ttgtagaata gccgtttata gcaattttaa gatattaaat ttatgagtcg tactaaaaaa
      661 acacgtcgta ttaccgacat tatgccggca agaaagaccg ataaacctaa acagcccatg
      721 cctaaattag gtggggggaa aaatcgcaaa ctgacccgtt atgagttaga tgctcaagcc
      781 cgtgaggaaa aacgtaagcg taaacataaa ggcttgccga caggctcacg caatgctgat
      841 ccggcagagc agaaaaaagc agtggtgaaa gaggtgaaag atccgcgtat cggtagccgt
      901 aaaaaagtgc cgttaatggt ggaatttgtg aaccagccgg aaaaagggcg gacgattaag
      961 gctgtgccgg ttgagccaat caaaccaacg ctttcaccgg aacaagagct ggagcagtta
     1021 gaaaataacg aatgtttaaa tcagttgtta gatgatttgg aggcgggcaa aaccttatcc
     1081 gcagaagatc agaaatttat gaatgaatgt ttagatcgca ttgatgaatt aatgactgag
     1141 cttggcatcg aatatgaaga tgaagagggc gataatggcg atgcgttatt acgccaattt
     1201 gaaacgatgg atattaataa attccgctag gtagccgatt atgatgctaa gaatattcct
     1261 gatggtgctt gccgctttga ttttaatttc gctgggcggg tatgcactgc acttaatgct
     1321 gaaagtgcga gctcagaaaa agcacgaaca agcactgatt gaacaggcaa agcaagcaca
     1381 aaaagatcgt tatattcgga ttttagacag cattgaagtt attgctcggg caatgatttc
     1441 agaacagtgc gatttttcgg aaggcgtttt acgtttaaaa ccgctattag atgttctcgg
     1501 cagaaaatta agcgactata cggcgatgtg ggcgttgtat ggcgtggtag aaaatatgcc
     1561 gattttagat gaacgcaaaa atttaaaacg taacgaaaga atgaagttgg atttagagcg
     1621 ggaagcaaaa gaagcggagc ttgaatcaga catcaaaaat gagtgttacc aattattaaa
     1681 agatattgaa gaaatgagaa aggcaatcta atgtcagaaa ttatttggga tttagccctt
     1741 attcaaaaat ataaccattc agggcctcgt tatacctctt acccaacagc gttggagttt
     1801 aacgaaaatt acaccaatga agattttatt cgtgctgcag ctcgttaccc tgagcgtccg
     1861 ctttcgcttt atgtgcatat tccgttttgc cataaactgt gctatttctg tggctgtaat
     1921 aaagtgatta ctcgtcatcg ccataaagtt gaaatttatt tagattattt agagcgtgaa
     1981 attaaaactc gcgcacctct gtttcaaaat cgtttagtga cccaaataca ctggggtggc
     2041 ggtacgccga cttatttaga cgaagatcaa tcggcacgtt taatgcaaat gctacgagcc
     2101 aattttgatg tgagcgatga tgcggaaatc agcattgaaa tggatccgcg tgaaatcgaa
     2161 ttaagtatgt tggatcacct gcgtgctatc ggttttaacc gtctgagtat gggtattcag
     2221 gatttcgata aagaagtgca acgtttggtg aatcgtgagc aagatgaaga gtttattttt
     2281 gccttgatga aacgggcaaa agaacttggt tttgtttcaa ctaatgtgga tttgatttac
     2341 ggcttgccaa aacagaatgt ggaaagcttt atgtacacct taaaacgtgt ggtagagcta
     2401 aaccctgatc gtatgagcgt atttaactat gcccacttgc caagccgttt tgctgcacaa
     2461 gtgaaaatta aagaagagtt gttgccggct ccggaaacca aattgacgat tttgcaaaat
     2521 tccatcgaat ttttaggcaa aaacggttat aagtttatcg gtatggatca ctttgctaaa
     2581 cctgatgatg agttagctat tgcacaagaa aaaggcattt tacaccgtaa cttccaaggc
     2641 tataccactc aagaagagtg tgatttattg ggaatgggcg tatcggcaat cagcttactc
     2701 ggcgatactt acgcccaaaa ccgcaaagaa ttacaacaat actatgctga tgttgaaacc
     2761 aacggtattg cgttgcataa aggcattgcg ttaacccaag atgattgcat tcgccgtgat
     2821 gtgataaaag ccttgatttg taatttcaaa ttagattttg ccaccattga aaagcaatat
     2881 ggtattgatt ttaaacgtta ttttgccgaa gatttagctt tcttagcgcc gttggcggaa
     2941 gatgaattat taacgatgaa tgacgacagc attgtggttt ccccacgagg gcgtttattg
     3001 atccgtaata tttgcttatg ctttgatgtc tattcaagac aattagcgag aagacagcag
     3061 ttttcccgta ttatttaatg agcaagcggt cgattttggt aaaaattttg caaaaacaga
     3121 ccgcttgttc ccggcttata aaggaaaaat gatgaaacag cgttatagct tactttctct
     3181 tgctgtgatg gcagcttgtt atagcaacag tgccgcagct gatttaaagc aacaatgttt
     3241 acttggcgtg ccgcattttc agggcgaagt ggtagagggc gatcaactta gaatgcctgt
     3301 taccattgag gctgacagtg cggtcattaa ccagcctcgt gatgccacct acaccggtga
     3361 tgtaacgatc aaacaaggta accgttcgat ttgggcggat caggtgcggg ttgagcaaga
     3421 tggcgaaaat gcccgaatgg cgtatttgca gggtaatttt aactaccaag ataatttgat
     3481 tcaggcaacc ggttcagatg cgaccatcaa tctgctttcc aaagatgcga atttagctaa
     3541 tgctaattac caattaattg gcagacaagg gcgtggcacg gcagaatccg gtgaattcgg
     3601 caacagcaaa cacacgctga aaaatgccag tttcacttcg tgcttaccga atgataattc
     3661 gtggcaaatt gaagcgaatg aaatgattca gcacgttgat gaagaatatg cggaaatgtg
     3721 gcacgcccgt tttaaagtgc tgggaatgcc ggtattttat tcgccttact tacaatttcc
     3781 gattggcgac cgccgccgta gtgggttatt aattccaagt tttgagcgtt caagcaaaaa
     3841 cggctggaca tattcgcaac cgttttattg gaatatcgca ccgaatatgg actcgaccat
     3901 cactccaact tactattcac gccgtggttg gcaaatcagc ccggaatttc gttatttaac
     3961 gaaaatcggg caaggcttag tggcaaccga atatatggcg aaagatcgtt taagcgagta
     4021 ccaaccggga aatgatgatc gtaaacgcta tttattccac tggcgtcacg caatgagttt
     4081 tttaaccgat tggcgtttct cggtcgatta caccaaagtg agcgacagac gttatttctc
     4141 tgattttgat tcggattatg gtagcagtac tgatggctat gcgacgcaga attttaaatt
     4201 aggctactat cagccgaatt ataatatctc aatttcaggt aaaaaattcc aaaccttcga
     4261 tgagttggat atcgggcctt atcgcgttct gccacaaatt gattttaact attataaaaa
     4321 cgatctaatt aaaggcggag atttcaaatt ctttgcccaa gcagcccgtt ttgaaaatga
     4381 tagtaaatta atgccaaaag cgtggcgttt ccacgctgag ccaacactga atttcccatt
     4441 agcgaaccgc tatggtagct taaatttaga aaccaagctc tatgcaacct actatcaaca
     4501 aacgaaaggt agtgagagta cggaagaaat taagaaaagt gtaagtcgtg tactgccaca
     4561 gattaagctc gattttaaaa ccgtgttgga atccgataaa cagcttttct ctggctatac
     4621 tcaaacgctt gagcctcggg tgcaatatat ttatcgccca tataaggatc aaagcgatat
     4681 tggctcgaaa gcacatcgaa gtgtgagctt aggctatgac tctgcattat tgcaaaacga
     4741 ctatttctca ctgtttaatg acagacgtta tagcggttta gatcgtattt cgtcagcaaa
     4801 tttagtcacc gcaggtggca caacccgttt ctttaaagag gcaaccggtg aagaggtgtt
     4861 taatttcagc ttaggacaaa cttattatat aagcccgtct aaaattgatg atacctcaga
     4921 taacagcact aaaggtcata cgacttcatg ggcagcggaa tctaactgga aattccataa
     4981 gaattggaat tggcatggta gctatcaata tgatactcgt ttaaaccaaa cggcgttagc
     5041 gaatatgtca ttgcaataca aaccacagtc agacaaattg attcagctta attaccgcta
     5101 tgcaagtaaa gattacatca atcaaaactt gaacacgaat aagtatgggc aatccattaa
     5161 acaagtgggc ggtgtgctag gttgggaatt aaccgataaa gtgtcggtaa tgaccagtta
     5221 ttaccacgac cttgcattga aaaaaccggt ggaagggcaa atttcgctta actacaacac
     5281 ttgctgttgg gcggcaaatg tgtatgtggc tcgtcgctta gtttcaacgc cggtaggttc
     5341 atctgatacg atcaatgatt tctactatga caataaattc ggcattaact ttgagctacg
     5401 ctttggcaca aactacagca atggtgtaac ccgtatgcta aaacgaggta ttattcctta
     5461 caccgagccg tatagcatta attaacaaag taaaaacaaa taagtaaaaa tacaaatcgg
     5521 ggcgaaaaat atttgccccg atttttttat atttggctga taagcggtta aattctgttg
     5581 gaattttgca aatcttccgc taaattcaac cgcttgccat cgaaacttaa cctgtatttc
     5641 gcatacctgc ggcaatacct gtgatggtga tcatcaatgc ttgctctagt acaggatcag
     5701 cattttcacc ttgtttgcga atcctatcta acaattctac ctgaagtaga ttgagcggat
     5761 cggtataaat attgcgtagt gcaatcgatt ctgcgatcca aggtaaatcg gccattaatt
     5821 gtccatcgtg agccagtgca agtacggttt taagatcagc ctgtagttgc gtacgcaaca
     5881 tattgcctaa acggtgtagt tttggctcga ctaggcgttg gtcgtaatat tccgccagcc
     5941 aaatatcagc tttactaaat accatttcca gcattccgat acgggtcgag aaaaacggcc
     6001 attcgttaca catttctttt agtaaggttt cattaccttc atcaatcaac tgttgcaatg
     6061 cttgccctgc acctaaccaa gcaggtagca ttaagcggtt ttgcatccaa gcgaaaatcc
     6121 aaggaattgc acgcaaactc tccactccgc cttttggatt acgttttgca gggcgtgagc
     6181 caagcggcag acgggaaagt tcttgctctg gtgtagcaga gcggaaatac ggtacgaagt
     6241 ctagctcgcc acgcaccata tcacggtaaa tttggcaaga aatatcggag gcttttgcta
     6301 gcacatctcg ccattgctgc tttggctccg gtggtgggaa aatattcgct tctaaaatcg
     6361 cactggcata gagttctaaa ctttcaactg ccacggcagg taagcctaat ttaaagcgga
     6421 tcatttctcc ttgttcggtc actcgtaagc catttttgag tgagcgaggt ggctgagaaa
     6481 gtaaggctgc gtgagccggt gcgccaccac gcccgatagt accaccacga ccgtggaata
     6541 aggttaattc aatattatat ttttcacata aattaaccag agcttcttgc gcccgatatt
     6601 gcgcccagcc ggcagcaagc attccggcat ctttggcgga atcagagtag ccgatcataa
     6661 tcatttggta attgttgatg acgccacgat accagccgat attaaataaa tcggtcatca
     6721 ctttttcact atgatctaaa tcgcctagcg tttcaaataa tggggcaacc ggaatagtaa
     6781 atttgcaccc cgcctctttg agtaaaaggt gaaccgagag tacatcagag gcctctcttg
     6841 ccattgaaat gacataggct gaaatgacac cttcaggttg ctcggcaaca attttacaag
     6901 tgtctaacac ttctttagtt tcagggcttg gagtccagtt ggttggaatc aatgggcggc
     6961 gagaactgag ttcacgcact aagaatgcct gtttatcatc ttccgtccag tgagcataat
     7021 cgcctaagcc gatatagcgg gtaatttctg caatcgccat ttcgtggcgg gtactttctt
     7081 ggcgaatatc taagcgtgat aaacctaaac caaaacagtt tatacgacgt aagcaatcta
     7141 gtaatgcacc atttgcaata attcgcatac cacaggcttg taatgactca tagcaatcaa
     7201 aaagcggttc ccacaattgc tgatctgaag ttaaaatgtc actttcatca atagttgaaa
     7261 ttttaccttc caataattcc gcatagtaaa tttcggtatt aattaatttc tggcgtaata
     7321 cttttagtaa tacgcgatat ggctctaaat ggttgccata tttagcacga aatgcagggg
     7381 tacactgtgt aatcgataat tcttcagcaa gatttttaat atcttgtaag aacaattccg
     7441 tggctttcca acggttcatt cttaatacac ggcgagttgt ttctgccgtg acgaacgggt
     7501 taccgtcacg gtctccgccc atccaagaag aaaaacggac aggggctaaa tttaccggat
     7561 attgcacacc aaagtgtttt tctaaataga agttaagctg acggcaaaat tccggcacgg
     7621 ctttccataa actattttca atgactgcca taccgccttt ggcttcatca acaggggttg
     7681 ggcgttgggt tctgatttca ttagtatgcc aagcaagggc aatcagttgc attaaacggc
     7741 gcttgatttt atagctttct gcctcagtta aatcatcatg ttccaacaaa ttcaaacatt
     7801 tattgatttc aacgtgttta tgccccagcg aacggcgggt tacttccgtt gggtgagctg
     7861 ttagcactaa atcaatcatt aggctttcca cggttttaat cactttttca acggggatat
     7921 tttgtgattt tagacgaagg aacagagagt ctaatgagcg actaccaagt gattcatcaa
     7981 tatgatggcg agaaacagtt tgatgttgtt cagcaatatt tgttagattt agaaaatggc
     8041 taaatgcacg tgcaacgggt aagacatcat cgttagaaat attggctaat ttatctaata
     8101 gctgttcacg ggcgttttca tcgccggcac gagaattgcg agataacaca cgaatgcttt
     8161 cgatgagttc tagaatgtaa tttccttgcg catcacgaat cgtttctccc aaaaattcac
     8221 ctaacatatg gatattatgc tgaattgttg aatattgctg gttcataaaa aattcctatg
     8281 gtaaacataa attaaattag gtctaaatat gccgaaaatc gctgaaaata gcaaatagat
     8341 ttaatttaat ttttgaatta aatgataaaa cgcaaaaaat agctgtcaat atgtgtttaa
     8401 aactgttgtt cggttttgat aacttgcctg ttgtttatgt gctattcact gttattcaag
     8461 ttggcttatc cattaatggt tttaaaggta ttaaatggtt ggaaaatgtt tcttgtgtgt
     8521 tcctcatcgg tattcttgct tatatgcttt atgttgtgaa tacaaaattc tctgtagaaa
     8581 taggcgagcg ctttgccaat attgaaggca cttggggaat gccattctgg gctgcaacta
     8641 cagcattctt gggtatttat tcaacaatga ttttaaatgc ttccgattat tctcgccacg
     8701 ttaaaaaagg aacaacccca acagcaacag gtgtgattta tgtattatca atcctccctg
     8761 taaccttatt tatgggttta atagggttgt tagttacggc agctacaggc aatagtgacc
     8821 caattgaagt atttgcaact gcaatggata ataaattttt aacagtaata acgttattat
     8881 tcattgcttt tgcacaggtt acgacaaacg tgttgaataa tattgttccg ccatcttaca
     8941 ttttaatgga ttcatttaaa atgaaatggt cgcatgccac cattttagtg ggcattcttt
     9001 ctatatgtgt aatgccttgg aaattggtaa ctgctgaatc agcagatgga ttatctttgt
     9061 ttattaaaat ttactcggca ttcttaggcc ctatttttgc ggtaatggtc gtcgattact
     9121 acatcttaag aaaacgtact ttaaacgtaa atgacttata taataaagaa ggtattttca
     9181 aagggattaa ctgggcagca attattgcga ttgctatcgg ttcgttttgc tcactgattg
     9241 tggtggatct ctcttggtat gtcagtttaa ttccatcagg cttgagctac tatattttga
     9301 tgagggtgtt aaaaagttcc gaagctttcc gtaaagggac agtgctagaa catcaataag
     9361 atgacaagcg gta
//
LOCUS       DANLYI010000087         9261 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.087, whole genome shotgun sequence.
ACCESSION   DANLYI010000087 DANLYI010000000
VERSION     DANLYI010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 9261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9261
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.087"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            <1..165
                     /locus_tag="PXD81_001461"
     CDS             <1..165
                     /locus_tag="PXD81_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014990986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF465 domain-containing protein"
                     /protein_id="HDL5537480.1"
                     /translation="HFARLFDKHNELDQRIKNMEANIELATHEEVEALKKEKLHLKDQ
                     LYAILKQESA"
     gene            complement(224..1381)
                     /locus_tag="PXD81_001462"
     CDS             complement(224..1381)
                     /locus_tag="PXD81_001462"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HDL5537481.1"
                     /translation="MSLDLADIRTQITNLDRNLLKLLAERHRLAFDVVRSKEITQKPL
                     RDLEREKILLQELVNFAEAENYQLDPQYVTQIFQRIIEDSVLTQQNYLQNKLNAQKEE
                     QVSIAFLGMRGSYSNMASRQFAKKYQGTLAELSCHSFNEVFEKVSLGEADFGVLPLEN
                     TTSGSINEVYDLLQHTDLTLVGELAYPIKHCVLANGTVDLAEVDTIYTHPQPAQQCSQ
                     FLAGLDKVHIKYCESSSHAMQMVARLNKPNIVALGNEDGGKLYGLTNIKTDIANQAHN
                     ITRFIVVAKEAVQVSPQVQTKTLLLMTTTQQAGALVDALMVFKNHHIRMMKLESRPIY
                     GKPWEEMFYVELEANIHSENTQKALAELEQVTSYLKVLGCYPSEIIEPVKL"
     gene            complement(1503..3569)
                     /gene="prlC"
                     /locus_tag="PXD81_001463"
     CDS             complement(1503..3569)
                     /gene="prlC"
                     /locus_tag="PXD81_001463"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HDL5537482.1"
                     /translation="MSNPLLNYTGLPAFSQIKPEHIKPAVEAVIKECRETVEKVAQIE
                     NPTWENFYMPQAMAEDKFSRAWSPVGHLNAVKNSPELREAYQACLPLLSEYSTWAGQH
                     QGLYQGYVKLKNSPEFETYSVAQKKAIENSLQDFELSGISLPAEKQKRYGEISARLSE
                     LNSQFSNNVLDATMGWDIVITDEAQLKGLPESALEAAKLSAESKGKKGYRFTLEIPSY
                     LPVMTYCENKALREEMYRAYVTRASDQGPNAGKWDNSAVIDEILKLRHEKAQLLGFKT
                     YSDYSLATKMAENPQQVLDFLNDLAARSKAQGKNELCDLKKFAKAHFGIEHLDLWDIP
                     FYSEKQKQALYSVNDEELRPYFPENRVLSGLFEVIKRIFGMRVEEQKDVDVWHKDVRF
                     FNIFDKNDRLRGSFYLDLYARENKRGGAWMDDCINQKRLADGTLQKPVAYLTCNFNKP
                     IGDKPALFTHDEVTTLFHEFGHGIHHMLTEIDVGDVAGINGVPWDAVELPSQFLENWC
                     WEEEALAFISGHYETGEPLPKAKLEQLLAAKNFQAAMFVLRQLEFGLFDFRLHTEYDP
                     SKQGQVLALLKEVKDQVAVAKGVEWARTPHSFSHIFAGGYAAGYYSYLWAEVLSADAY
                     SRFEEEGIFNPETGQSFLDNILTRGGSEEPMVLFERFRGRKPTLDALLRHKGIVGNAE
                     PVDCTQ"
     gene            complement(3700..4440)
                     /gene="pflA"
                     /locus_tag="PXD81_001464"
     CDS             complement(3700..4440)
                     /gene="pflA"
                     /locus_tag="PXD81_001464"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005604771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HDL5537483.1"
                     /translation="MSVVGRIHSFESCGTVDGPGIRFILFLQGCLMRCKYCHNRDTWD
                     LDGGKEITVEELMKEVTTYKHFMKATGGGVTASGGEAVLQMEFVRDWFRACKAEGINT
                     CLDTNGFVRHYSPVVDEMLEVTDLVLLDLKQLNDEVHQDLVGVSNKRTLDFARYLHKL
                     NKPTWVRYVVVPGYTDDDDSAHRLGQFIQGMDNIEKVELLPYHRLGAHKWETLGYKYE
                     LEGVMSPPKEDLERIQKIIESYGHTVKF"
     gene            complement(4689..5714)
                     /locus_tag="PXD81_001465"
     CDS             complement(4689..5714)
                     /locus_tag="PXD81_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence RhuM family protein"
                     /protein_id="HDL5537484.1"
                     /translation="MSKQDLIRSSVAEYLTFITATGESQVDAVYADENVWLSQKMMAV
                     LYDVDVRTISYHLKKIFSDNELAENSVIQFFWITAQDGKNYHTKHYNLSAIIAVGYKV
                     NSERAVQFRKWATEIVHSFAIKGFAMDDERLKNDGTILGKKYFEEQLARIREIRLSER
                     KFYQKITDIYATAVDYDRTATATKRFFATVQNKLHWAIHGHTAAELIMARANAEKPNM
                     GLTNWKDAPQGKIYPFDVVVAKNYLSDNELAQLQRLVSAYLDMAEDMAERQIPMTMAD
                     WETRLNRFLAATDREILQDAGKVTAEIAKSFALSEFEKYRVKQDLTYESDFDLLVKEV
                     AAKYHAG"
     gene            complement(5949..8273)
                     /gene="pflB"
                     /locus_tag="PXD81_001466"
     CDS             complement(5949..8273)
                     /gene="pflB"
                     /locus_tag="PXD81_001466"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005601494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HDL5537485.1"
                     /translation="MTQLTDAQQKAWEGFAAGEWQTEVNVRDFIQKNYTPYEGDESFL
                     AEVTPATSKLWADVMEMIKVENKTHEPYDIDCDTPSTITSHAPGYIDKSLEKIVGLQT
                     DAPLKRAIMPFGGINMVKGSCKVYRRELKPEVEQIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVITGLPDAYGRGRIIGDYRRMALYGADFLMKDKQRQFASLQARLESGEDIQATI
                     QLREEIAEQHRALGKMKEMAASYGFDISGPATNAQEAVQWTYFAYLAAVKSQNGAAMS
                     FGRVSSFLDIYIERDLKAGKITEQEAQELIDHLVMKLRMVRFLRTPEYDQLFSGDPMW
                     ATETLAGMGLDGRTLVTKNSFRILHTLYTMGPSPEPNLTILWSEQLPEAFKRYCAKVS
                     IDTSSVQYENDDLMRPDFQNDDYAIACCVSPMIVGKMMQFFGARANLAKTLLYAINGG
                     VDEKSGDQVGPKTEAITSEYLDYDDVMTRLDSFMDWLATQYVTALNIIHFMHDKYAYE
                     AALMALHDRDVFRTMACGIAGLSVAADSLSAIKYAKVKPIRTDIEIKNKAGEVVGVAK
                     NVATDFEIEGEYPQFGNNDNRVDDIAVDLVERFMKKIQKLKTYRNATPTQSVLTITSN
                     VVYGKKTGNTPDGRRSGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTF
                     SIVPNALGKDYEAQKRNLAGLMDGYFHHEATVEGGQHLNVNVLNRDTLLDAVEHPEKY
                     PQLTIRVSGYAVRFNSLTKEQQMDVITRTFTESM"
     gene            complement(8363..9187)
                     /gene="focA"
                     /locus_tag="PXD81_001467"
     CDS             complement(8363..9187)
                     /gene="focA"
                     /locus_tag="PXD81_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012263076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HDL5537486.1"
                     /translation="MFSPAEMAKIAEDGAVYKATKNQLYSFLSAITAGGFIAIAFVFY
                     TTTQVGASQMPWGMAKLIGGLVFSLGVIMCVVFGAELFTSSTLTIVARASQRINWLQM
                     FKNWVVVYFGNFVGAISIVLLIWFSGQIMAAKGQWGLVILNTAQHKIHHQWFEAFCLG
                     IFCNIMVCIAVWMSYAGKSLTDKAFIMMLPIAMFVASGFEHCVANMFMIPMGIAISHF
                     ASPEFWTAINVDPTQFADLDVSHFVFKNLIPATLGNIVGGVFFIGLMQWFLYIRKH"
BASE COUNT         2592 a         2095 c         1832 g         2742 t
ORIGIN      
        1 cattttgctc gcttatttga taaacataat gagctagacc agcgtattaa gaatatggag
       61 gcaaatattg aactggcaac acatgaagaa gttgaggcac taaaaaaaga aaaattgcat
      121 ttaaaagacc aactttatgc aattctaaaa caagaatcgg cttaacctaa agtaaaagct
      181 cagaaaatat tctgagcttt aatttcggtc atattagaca atattacagt tttaccggct
      241 caataatttc gcttgggtag caacccaaca ctttgaggta gctggtaact tgctccaatt
      301 ccgccaatgc tttttgggtg ttctcggaat gaatatttgc ctccagctcc acataaaaca
      361 tctcttccca aggtttgccg taaatcgggc gggattccag tttcatcata cgaatatgat
      421 gatttttgaa taccattaac gcatcgacca atgcacccgc ctgttgcgtg gtggtcatta
      481 acagtaacgt tttggtttgt acctgtggcg aaacctgcac agcctctttg gcaacaacaa
      541 taaaacgggt gatattgtga gcttgattag caatatcggt tttgatattc gtcaagccat
      601 acaattttcc gccatcttca ttgcccaagg caacaatatt cggcttattc aagcgagcca
      661 ccatttgcat tgcgtgcgag ctgctttcgc aatatttgat atgtacttta tctagcccgg
      721 ctaaaaattg gctacattgc tgggcaggct gagggtgggt gtaaatggta tccacctccg
      781 ccaaatcaac cgtgccgttt gccaatacac aatgtttgat tgggtacgcc aattcaccca
      841 ctaaggttaa atcggtatgc tgcaataaat catacacttc attgatcgaa cctgaagtcg
      901 tgttttcaag cggcagtacg ccaaaatcgg cttcaccaag gctgactttc tcaaatactt
      961 cattaaaaga atggcagctt aattccgcca atgtgccttg atattttttg gcaaactgtc
     1021 gagatgccat attagaataa gagcctcgca ttcccaaaaa cgcaatcgaa acctgctctt
     1081 ctttttgtgc attcaattta ttttgcagat aattttgctg agtcagcact gaatcttcaa
     1141 taatacgttg gaaaatttgg gtcacatatt gcggatcaag ctggtaattc tccgcttcgg
     1201 caaaatttac cagttcttga agcaaaatct tttcccgttc taaatctctc aagggttttt
     1261 gtgtgatttc tttgctccgc accacatcaa aggctaagcg gtggcgttct gccagtagct
     1321 ttaataaatt gcggtctaag ttggttattt gggttcgaat atcggctaaa tctaaagaca
     1381 ttatttcact ctctgtcagg ctaaatgctg aaaattataa cgagttgcaa aagaaagaaa
     1441 agacaagcgg tcaaatttac aaaaaaattt gcaaatctaa ccgcttgtaa aacgtgttta
     1501 ctttattgag tgcaatcaac aggctcggca ttacctacaa tgcctttgtg tctgagcaag
     1561 gcatcaaggg ttggcttacg cccacggaag cgttcgaaca gcaccattgg ctcttctgaa
     1621 ccgcctcggg ttaagatatt atctaagaaa gactgccccg tttcagggtt gaaaatcccc
     1681 tcttcctcaa agcgtgaata ggcatctgct gaaagcactt ccgcccataa ataactgtag
     1741 tagcccgccg catagccgcc tgcaaaaata tgcgagaaac tgtgtggcgt tctcgcccac
     1801 tctacgcctt tggcaacagc gacttggtct ttcacctctt taagtaaggc taacacttgt
     1861 ccttgtttgc tcgggtcata ttcggtgtgt aggcggaaat caaacaagcc aaattctaat
     1921 tggcgaagca caaacattgc cgcttggaag tttttcgccg ccaataattg ctctaatttc
     1981 gcttttggta acggctcgcc ggtttcataa tgccctgaaa taaaggctaa tgcctcttct
     2041 tcccagcacc agttctctaa gaattggctt ggcagctcca ctgcgtccca cggcacaccg
     2101 ttaatgccgg cgacatcgcc cacgtcaatt tcagttagca tatggtgaat gccgtgtcca
     2161 aactcgtgga acaaagttgt cacttcatcg tgggtaaaca atgccggttt atcgccaatc
     2221 ggtttattga agttacaggt taaatatgcc accggttttt gtaatgtgcc gtcagccaag
     2281 cgtttttggt tgatgcaatc atccatccac gccccaccac gtttgttttc acgagcgtat
     2341 aaatctaagt agaacgagcc acgcaagcgg tcatttttat caaaaatatt gaaaaaacgt
     2401 acatctttgt gccatacatc cacatctttt tgttcttcca cacgcatacc gaaaatgcgt
     2461 ttaattactt caaataagcc tgataatacg cgattttccg ggaaatacgg gcgaagttct
     2521 tcatcattga ccgaatacaa tgcctgtttt tgtttttcgc tgtagaacgg aatatcccat
     2581 aaatccaaat gttcaatgcc gaaatgggct ttagcaaatt ttttcaaatc gcataattca
     2641 tttttgcctt gagctttcga gcgagcggct aaatcgttca agaaatctaa tacctgttgt
     2701 ggattttctg ccattttggt ggctaatgaa taatcagaat aagtcttaaa gccgagaagt
     2761 tgggcttttt cgtgacgcag ttttaagatc tcatcaatca ccgcactgtt atcccatttg
     2821 cctgcattcg ggccttgatc agaagcacga gtcacataag cacggtacat ttcctcacgc
     2881 agtgctttgt tttcacaata cgtcatcacc ggcaaatagc ttgggatttc aagagtgaag
     2941 cgatagcctt tcttaccttt gctttctgcg gataattttg ccgcttctaa tgccgattcc
     3001 ggcaagcctt taagctgtgc ttcatcggta attacaatat cccaacccat tgttgcatca
     3061 agtacgttgt tgctaaattg cgagttgagt tccgacaagc gtgcagaaat ttccccgtag
     3121 cgtttttgtt tctctgccgg cagtgaaatg cctgataatt caaaatcttg taagctgttt
     3181 tcaatcgcct ttttctgtgc aaccgaataa gtttcaaact cagggctgtt ttttaacttc
     3241 acatagcctt gatataaccc ttgatgttgc cccgcccaag tgctgtattc cgataataac
     3301 ggcaagcacg cttggtaagc ctcacgcaac tctggggaat ttttcaccgc attcaaatgc
     3361 cctaccggtg accacgcacg ggagaattta tcctctgcca ttgcttgtgg catatagaaa
     3421 ttttcccacg tcgggttttc aatttgtgcg actttttcaa cagtttcacg gcattctttg
     3481 atcaccgctt ctaccgccgg tttgatatgt tcaggtttga tttgtgagaa cgcaggcaag
     3541 cctgtgtaat ttaaaagagg gttagacatt attttttcct tttatggtca aaatgaaaat
     3601 tcgtatcaag ataatacaca aagtgagaga gaaaagaaaa caagcggtca tatttcaata
     3661 attttttgca aaaaaatcac aaaatatgac cgcttgtctt tagaatttga ctgtatgccc
     3721 gtaactttca atgatttttt ggatacgctc taaatcctct ttaggcggtg acattacccc
     3781 ttccagctca tatttatagc ctaaggtttc ccatttgtga gcccctaaac gatgataagg
     3841 cagaagttcc actttctcaa tgttatccat tccttgaata aactgcccta aacgatgggc
     3901 agaatcatcg tcatcagtat aacccggcac gaccacatag cgaacccaag tcggtttatt
     3961 taatttgtgc agataacggg cgaaatcaag ggtacgctta ttcgacaccc cgactaaatc
     4021 ttggtgaact tcatcattga gctgttttaa atccagcaag accaaatcgg tcacttcaag
     4081 catttcatcc accaccgggc tataatggcg aacaaaacca ttggtatcta agcaggtatt
     4141 aatgccttca gccttacagg cacgaaacca atcccgcaca aactccattt gcaacaccgc
     4201 ttccccaccc gaagctgtta caccaccgcc ggttgctttc ataaaatgct tataagtcgt
     4261 gacttctttc atcagctctt caaccgtaat ttccttacca ccatcaagat cccaagtatc
     4321 acgattatgg caatatttac accgcatcaa acagccttgc aagaacagaa taaaacgaat
     4381 ccccggtcca tctaccgtac cgcaggattc aaaagaatgg atacgtccaa caacagacat
     4441 ttgtcacctc aaccctcaaa aaattttgag aaattgtagc aaatttttat gttgttcgca
     4501 gaaataaatc acaccggtat attttaagaa aggagaaagt catactattt tttgcctatt
     4561 tcaggaataa acttttatta atctattgac tccatcaaca tatcaaccaa atatgtttaa
     4621 atttttgcat tttttaaagc tattttgacc gcttacaagc agtcggtttt tctgaaaact
     4681 ttgcaaacct agccggcgtg gtatttggcg gcgacctctt ttaccagtaa gtcgaaatcg
     4741 ctttcgtagg tgagatcctg cttgactcgg tatttttcaa attcgctgag ggcgaaagat
     4801 ttagcaattt cagcggtaac tttgccggca tcttgtaaga tttctcggtc ggtggcggca
     4861 agaaagcggt taaggcgggt ttcccagtct gccattgtca tcggaatttg gcgttctgcc
     4921 atatcttccg ccatatccaa ataggcagag accagccgtt gtagttgtgc taattcatta
     4981 tccgataaat aatttttggc aaccaccaca tcaaaggggt aaattttgcc ttgcggagcg
     5041 tctttccaat tcgttagccc catattcggc ttttcagcgt tggctcgtgc cataatcagc
     5101 tcggcggcgg tatgcccgtg aattgcccaa tggagcttgt tctgcacggt ggcaaaaaag
     5161 cgtttggtgg cggttgcggt gcggtcgtag tccacggcgg tagcgtaaat gtcggtgatt
     5221 ttttggtaga acttgcgttc ggataaccga atttcccgaa tacgagcaag ctgttcttca
     5281 aagtattttt tgcccaaaat cgtgccgtca tttttcagcc gttcatcgtc catcgcaaag
     5341 cctttaatcg caaagctatg cacgatttcg gttgcccatt tgcgaaactg caccgctcgc
     5401 tcggaattga ctttataacc caccgcaata attgccgaca gattataatg cttggtgtgg
     5461 tagtttttgc cgtcttgggc agttatccaa aaaaattgga taactgaatt ttcagctaac
     5521 tcattgtcac taaagatttt tttgagatga tagctaatgg ttcgtacatc aacatcatac
     5581 aacaccgcca tcattttttg gctaagccat acattttcat cggcgtaaac ggcatcaact
     5641 tggctttcgc cggtagctgt aataaaagtg aggtattccg ccacagagga acgaattaaa
     5701 tcttgcttag acatctcttt ctcccatata gaacactgta attataaaca atattacgaa
     5761 agctgtgtaa atgaatagcc aatttttcga gcgagatcac aaaaaaaccg cttgcggcat
     5821 aaatgctaca agcggttgat tattactcac aaaattgttt ccaattttgt tcgctcctac
     5881 ggagccgtcg gcaaagccaa cgttcaaaac gcaatgcgtt ttgtggctaa aagttttgca
     5941 aattgtaatt acatactttc agtgaaggtg cgggtaatta cgtccatttg ttgctctttg
     6001 gttaaagagt tgaaacgtac cgcatagcct gaaacacgaa tcgttaattg cggatatttt
     6061 tcagggtgtt ctaccgcatc taataatgta tcacggttta acacgttcac atttaagtgc
     6121 tgaccgcctt ctacagttgc ttcgtggtgg aagtagccgt ccattaagcc tgcaaggtta
     6181 cgtttttggg cttcgtaatc tttacctaac gcatttggta cgatagagaa agtataagaa
     6241 ataccatctt tcgcataggc aaacggtaat ttcgccacag aagttaatga agctaccgca
     6301 cctttctggt cacgaccgtg cattgggttc gcacccggtc cgaatggagc acctgagcga
     6361 cgaccgtccg gtgtattacc ggttttctta ccatatacca cgttagaagt gatagtaagc
     6421 acagactgag tcggcgttgc attgcggtat gttttgagtt tttgaatttt cttcataaaa
     6481 cgttcaacta aatcaactgc aatgtcgtcc acacggttat cgttgttacc gaattgcgga
     6541 tattcaccct caatttcaaa gtcggttgct acgtttttcg ctacaccaac cacttcgcca
     6601 gctttgtttt tgatttcaat atcggtacgg attggtttta ctttcgcata tttaatggct
     6661 gaaagtgagt ctgccgctac tgaaagccct gcgataccac acgccattgt gcggaacaca
     6721 tcacggtcgt gaagagccat taatgccgct tcgtatgcat atttatcgtg cataaagtgg
     6781 ataatgttca gtgctgtcac atattgggtt gcaagccaat ccataaagct gtctaagcgg
     6841 gtcattacat catcgtaatc taagtattca cttgtaattg cttcagtttt aggccctact
     6901 tggtcgcctg atttctcatc aacaccgcca ttgattgcgt ataacaaggt tttcgctaag
     6961 tttgcacgag cgccgaagaa ctgcatcatt ttacccacga tcattggcga tacgcagcac
     7021 gcaatcgcat agtcatcgtt ttggaaatcc ggacgcatta agtcatcgtt ttcatattga
     7081 actgatgaag tatcaataga cactttcgca cagtagcgtt taaatgcttc cggtaattgt
     7141 tcagaccaaa gaatagttaa gtttggctcc ggtgacggcc ccattgtgta aagggtgtgt
     7201 aaaatacgga agctgttttt ggttactaag gtacgaccgt ctaaacccat accggctaag
     7261 gtttcggttg cccacattgg gtcgcctgag aataattgat cgtattccgg agtacgcaag
     7321 aaacgcacca tacgcaattt cattactaaa tggtcgatta actcttgggc ttcttgctcg
     7381 gtaattttgc cggcttttaa gtcacgttcg atataaatat ctaagaatga agacacacga
     7441 ccgaaagaca tcgccgcacc gttttgcgat ttaaccgctg caaggtaagc gaaatatgtc
     7501 cattgcaccg cttcttgtgc attggttgca ggacctgaaa tatcaaaacc gtagcttgca
     7561 gccatctctt tcattttgcc taaagcacgg tgttgctcgg caatttcttc acgcagttga
     7621 atcgtggctt gaatatcttc gccggattct aaacgagctt gtaatgaggc aaactggcgt
     7681 tgtttgtctt tcattaagaa gtctgcacca taaagtgcca tacggcggta gtcaccgata
     7741 atacgaccac gaccataagc atccggcaaa ccggtaatta cacctgattt acggcaacgt
     7801 aaaatatccg gagtgtaaac atcgaatacc ccttggttat gggttttacg gtattcggta
     7861 aagatttgtt ctacttcagg tttaagctca cggcgataaa ctttgcacga acctttcacc
     7921 atattgatac caccaaacgg cataatcgca cgttttaatg gagcatctgt ttgcagacca
     7981 acgatttttt ctaacgattt atcaatataa cccggtgcgt gtgaggtaat ggttgatggc
     8041 gtgtcgcagt caatatcata cggttcgtgt gttttgtttt ctactttgat catttccatc
     8101 acatctgccc acaatttgct ggttgcaggt gttacttcag ctaggaaaga ctcatcaccc
     8161 tcataaggtg tgtagttttt ttggataaaa tcacgtacat ttacttcagt ttgccactcg
     8221 cctgcggcaa aaccttccca cgctttttgt tgagcgtctg ttagttgagt cattttaagt
     8281 cctctgtgtt agtaagttaa aacgggaaag cggtgatttt ttgcaaaaaa tttgcaaaat
     8341 ttgaccgctt gaatctgaaa tcttaatgct ttctaatgta taagaaccac tgcattaaac
     8401 caatgaaaaa cacgccaccg acaatattac ccagcgttgc cggaattaag ttcttaaaca
     8461 caaaatgaga tacatctaaa tcggcaaatt gcgttgggtc aacattaatt gctgtccaaa
     8521 attcagggct ggcaaagtgc gaaatcgcaa tccccatcgg aatcataaac atatttgcca
     8581 cacagtgttc aaagccggaa gccacaaaca ttgcaatcgg taacatcata ataaaggctt
     8641 tatcggttag gcttttacct gcataagaca tccacaccgc aatgcacacc ataatgttac
     8701 agaaaatgcc taaacagaaa gcctcaaacc attggtggtg aattttgtgc tgtgcggtat
     8761 ttaaaataac caaaccccat tgccctttgg ctgccataat ctgaccacta aaccaaatca
     8821 acaatacaat ggaaatcgcc ccaacaaagt taccgaaata caccactacc cagtttttaa
     8881 acatctgtaa ccagttaatt ctttggctgg ctctggctac aatcgtaagt gttgatgagg
     8941 taaaaagttc tgccccaaac accacacaca taatcacgcc gagtgaaaat accaaaccac
     9001 caatcagttt agccataccc caaggcattt ggctcgcacc cacttgggtt gtggtataaa
     9061 atacaaatgc tattgcaata aaaccgcccg cagtaatggc agagagaaag gaatataatt
     9121 gattttttgt tgctttataa accgcaccat cttctgcgat ttttgccatt tcagcaggag
     9181 aaaacatcag acaaccttaa caatgagaat tgaaaaaccg atatattatg tagaaattat
     9241 agacctcttg agataatctt c
//
LOCUS       DANLYI010000088         9218 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.088, whole genome shotgun sequence.
ACCESSION   DANLYI010000088 DANLYI010000000
VERSION     DANLYI010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 9218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9218
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.088"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            <1..6051
                     /locus_tag="PXD81_001468"
     CDS             <1..6051
                     /locus_tag="PXD81_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HDL5537487.1"
                     /translation="IFNQTTQKMEVVSELARSQGKAASSTDKRGTIKLAAASILGGAT
                     LISGMAAFMLSATPAEAAVIIDRIEVQQKAPKKVVNGGIDIYTNTSLATDSITNPYNY
                     FNPGSLSYADRTKLQESNNSVNKEKDSRLLYKSNTTSAIVIGHNAVALDNNRKSRGKP
                     KASGIAIGDYSYSGGGLSIAIGSFSRTASIGSVALGTSARAEGFNSFAAMRQSAAIAD
                     YSTAIGSTSWANATASFAIGASATALGDRSFAIGSGTPQTVDGSYGHENRRSIYDGQN
                     NTLAFGTDAFSIGTKAKTNGNHSFAIGTNAWSGGFKLENSNISELGQVINPDTAKVAN
                     NAFALGTNTRANSNDTLAFSTNASATGDGSIAFGVNSNTTGIGAIAFGNASKAWKSNA
                     ISIGWNANSHAEKVISIGSHSNASKDGAMAIGDTANSSGNNSVAIGYSANVSKANATA
                     VGTRANITHEGGTAIGFEAKSKGNYATAIAYNSTAKGKNSIAAGVNSTVSGIRAAALG
                     YNNTVSGKDTFVLGSNVTVKTKGSVILGSDSTEATATKEDTATVGTLKYSSFAGDENV
                     VDGDYVSVGNATNARQIKFVAPGKIASNSTDAINGSQLYAVADTLVKTGFNITADHSG
                     LGGGAKEDNVKLGETVKYTSTDKNVVTTVTNNTIDFGLSDNISVGGKPGKDGSVGVKG
                     ADGKDGVTIKPEAIVFHGVDGVNGKDGKDGKASIKVEKGAKGLDGNDGKDGESKTRIV
                     YEKPNGDKEEVATLNDGLKFGANDGKVHDAKLNTQVDIKGATTNTDWAKFDAGKNIMT
                     QVVGNTITVALAKDLAGLDSATFGTQGDSTVINKDGVTIAKKDPNDATKSNVTLTEDG
                     LSNGGNQITNVDSGLKGKDGQPVKLTEAAGDVLNNAVNVGDLKNTVTETVTEVTTDAF
                     GLKDENNKEFKQNLGTTAKIIGDGNVKTNVVEKDGGHALEVSLVNNITVGKDGEPGKV
                     GIAGKDGKDAIALDGKDGAITVGRPGKDGIDGTIGVNGKDGSAVVLNGKDGSIGLNGT
                     NGKDGLTIKGTEGKPGLVGKDGLPGKDGVTRIQYQPTDGNGDPIGEPEQVATLNDGLH
                     FKGDDATLVSKKLNETLEIIGGADKDKLTANNIGVNSKDGKLSVQLAKDLKDLNSATF
                     KPQDGENGQEGKGPTTVVNNNGVTITPKDGTDGKAVKLTEDGLDNGGNQIVNVKSGID
                     GLAGKDGINGKDGKPITSVNDLTDDQLKEVGTNAANINDVVNASNDIIEKGFSIAADN
                     GETDHVKLGETVKYTSKDKNIITTVSDNVIDFTLNNDFTIGGPGKDGSIGVKGADGTV
                     GVTLNGKDGSIGLTGPKGADGKDGASATISVKDGAKGLDGNDGKDGESKTRIVYEKPD
                     GTTEEVATLNDGLRFVGDDGKEIVKKLNETLKISGGDTDLTNLSDNNIGVVNNNGALT
                     VKLAKDIDLTKDGSVTIGDTTVNNDGLTIKDGPSVTKAGINAGDKKIINVQDGEISAT
                     SKDAVNGSQLYQVQEVANAGWNLTANGKDKGNVKPGATVDLNNKDGNIIITKEGNNVT
                     FGLKNNLTIGGKDGKDGQIGIAGKDGKDGVTIKGDGTITAGRDGKDGVDGSIGATGKD
                     GASVVLNGKDGSIGLTGPKGADGKDGASANIAVKDGAEGVDGTNGKDGLPGENGKTRI
                     VYETKDKDGKTVTEQVATLNDGLIFSGNNEDVKNRQKLNTEVKVKGEGVDKTASENFK
                     SASGNINVKANGTDTLEVQLAKAIDLTEDGSVTTGDTTVNNNGLTIKDGPSVTKGGID
                     AKGTKITNVKDGDVTATSKDVVSGSQLYNAMQNTGWYLTVDGNVAETSEKGAQRVNNN
                     GKVAVSGGKNIVVSRKGSTVEVATSSNLEFDSVKVGGTTISSTVAKDGVNELNIAGSK
                     NAQTRITNVAPGVKGTDAVNVNQLKGAENRLNNRISNVDKDLRAGIAGALAAGNLYHV
                     TQPGKSMVSAGVGAYRGQGAIAVGYSRLSDNGKVGIKFSVNSNSRGDTGAAASVGYQW
                     "
     gene            complement(6273..7658)
                     /gene="hflX"
                     /locus_tag="PXD81_001469"
     CDS             complement(6273..7658)
                     /gene="hflX"
                     /locus_tag="PXD81_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HDL5537488.1"
                     /translation="MLNESLPNSEENSAFGFALSEASQTTEAASPTDPENQDKAILVH
                     LYLGQHKDVENLLEFKTLAESAGVEVLATLTTSRQSSHIKYYVGQGKAEGIAEAVERL
                     GATVVLVNHQLSPAQTRNLQSLCGCRVVDRTGLILDIFAQRARSHEGKLQVELAQLRH
                     LSTRLVRRVTNQDQQKGGAVGLRGPGETQLETDRRLIKVRIQQLLNRLEKVSKQRNQN
                     RKTRQKADIPTVSLVGYTNAGKSTLFNAITQASVYAADQLFATLDPTLRRIQVQDVGT
                     TILADTVGFIRFLPHDLVSAFKSTLQETTEATLLLHVIDGSDERKNENIDAVNQVLDE
                     IGALDIPTLLVFNKIDKLEGVEPHIERNADGTPVAVYLSAQENSGIELLFQAIQERLR
                     SNLVCENLLLPVTAGAIYAQFKAENCIKTEHFNEFGERIISIEINEVQWNKWVKQFPD
                     LAEYIELAKWG"
     gene            complement(7678..7950)
                     /gene="hfq"
                     /locus_tag="PXD81_001470"
     CDS             complement(7678..7950)
                     /gene="hfq"
                     /locus_tag="PXD81_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005712219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HDL5537489.1"
                     /translation="MAKGQSLQDPYLNALRRERIPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPARALSHNNNNQAQHAQPAAEVTEQAE"
     gene            complement(8019..8972)
                     /gene="miaA"
                     /locus_tag="PXD81_001471"
     CDS             complement(8019..8972)
                     /gene="miaA"
                     /locus_tag="PXD81_001471"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HDL5537490.1"
                     /translation="MNKKPLALFLMGPTASGKTDLAIALRQELPVEVISVDSALIYKG
                     MDIGTAKPSKEELALAPHRLIDILDPAESYSAMNFHADALREMADITSQGKIPLLVGG
                     TMLYYKALLEGLSPLPSADPAIRAEIESRAAQHGWATLHQELVKIDPIAGARINPNDS
                     QRINRALEVFYITGKTMTELTAQQGEALPYNILQFAIAPQDRAVLHQRIEQRFHKMIE
                     LGFEEEVKRLFLRKDLHINLPSIRCVGYRQMWEYLQGDISLDEAIFKGICATRQLAKR
                     QITWLRGWQGELTWLDSLAPTSSKAKMVEKIDHYLKNCDNI"
BASE COUNT         2855 a         1654 c         2150 g         2559 t
ORIGIN      
        1 atttttaacc aaacgacgca aaaaatggaa gttgtgtctg aacttgctcg atcgcaaggt
       61 aaagccgctt cttctactga taagcgcggt actattaaat tagcagcggc tagcattcta
      121 gggggagcaa cccttatcag tggaatggca gcctttatgc tttctgctac accggcagaa
      181 gcggcggtga ttattgatag aatagaagta caacaaaaag caccaaaaaa agttgttaat
      241 ggtggtatag atatttatac aaacactagt ttggctacag attctattac gaatccatat
      301 aactacttta acccaggtag cttatcctat gcagacagaa ctaagcttca agaatctaat
      361 aactctgtta ataaggagaa ggattctaga ttattgtata agtctaatac tacctcagca
      421 attgtaattg gacacaatgc tgtagcttta gacaataaca gaaaaagtag aggaaaaccg
      481 aaagcgtcag gcatagccat tggtgattat tcatattctg gagggggatt gtctattgca
      541 attggttctt tttctcgtac tgcaagtatt ggttctgtag cattaggtac ctctgcacgt
      601 gcggaaggat ttaactcatt tgctgcaatg cgtcaatctg ctgcaattgc agattattct
      661 actgcaattg gttcaacatc ttgggcaaat gctacagcta gcttcgccat tggtgcttcg
      721 gctacagcgc taggtgaccg ttcttttgcg attggtagtg gtactccaca gacagtggat
      781 ggaagttacg gtcatgaaaa tagacgttca atttatgatg ggcaaaataa tactttagca
      841 tttggaacag atgcattctc aatcggtact aaagcgaaaa ctaatggtaa ccattcattt
      901 gccattggta ctaacgcttg gtccggtggc tttaaattag agaattcaaa tatttctgaa
      961 ttaggacagg taattaatcc tgatacagcg aaagtagcaa ataatgcatt tgctttgggt
     1021 acgaatactc gtgcaaattc gaatgatact ttggcgtttt caacaaatgc aagtgcaaca
     1081 ggtgatggtt ctatcgcctt tggtgtaaat tcgaatacaa caggaatcgg tgcgattgct
     1141 tttggtaatg cttctaaagc ctggaaaagt aatgctatta gcattggttg gaatgcaaac
     1201 tctcatgcag aaaaagtgat cagtattggt tcgcattcta acgcgagcaa agatggtgca
     1261 atggcaattg gtgatacggc taattcaagt ggtaataatt ctgttgcgat aggttatagt
     1321 gctaatgtaa gtaaggcgaa tgcgacagca gtaggtacac gtgccaatat tacgcatgaa
     1381 ggtggcacag caattggttt tgaggccaaa tcaaaaggta attatgcaac tgctattgct
     1441 tacaactcaa ccgcaaaggg taaaaactca attgcagccg gtgtaaatag tactgtttcg
     1501 ggtattcgtg ctgcggcttt aggttataac aatacagttt caggtaaaga tactttcgta
     1561 ttaggttcta atgttactgt taagacaaag ggttctgtaa ttctaggttc tgactctact
     1621 gaagcaaccg caactaaaga agatactgca actgttggca cattaaaata tagcagcttt
     1681 gctggcgatg aaaatgttgt tgatggtgat tatgtgagtg tgggtaatgc aaccaatgct
     1741 cgccaaatta agtttgttgc accgggtaaa attgcttcca attcaacaga tgcgattaac
     1801 ggttcacaac tttacgcagt agctgacact ttagttaaaa ctggctttaa cattacagcg
     1861 gatcactctg gcttaggagg tggtgctaaa gaagacaatg tgaagttagg tgaaactgtt
     1921 aagtacacct caaccgataa gaatgttgtt acaaccgtaa caaataatac catcgatttt
     1981 ggcttatccg ataacatctc tgttggtggt aaaccaggta aagatggttc agttggtgtt
     2041 aaaggtgctg atggtaaaga tggtgtaaca attaaaccag aagccatcgt attccacggc
     2101 gttgatggcg taaatggaaa agatggaaaa gatggtaaag catcaattaa agttgagaaa
     2161 ggtgctaaag gtcttgacgg caacgacggt aaagacggcg aaagcaaaac tcgtattgtt
     2221 tacgaaaaac caaatggtga taaagaagaa gttgcaacac ttaacgatgg cttaaaattc
     2281 ggtgcgaatg atggcaaagt tcacgatgcg aaattaaata cgcaggtgga tattaaaggt
     2341 gctacaacaa acactgattg ggctaagttt gatgctggta aaaatatcat gacacaggtt
     2401 gttggcaata ccattactgt ggcgttagca aaagatctag caggcttaga tagtgcaaca
     2461 ttcggcacac aaggtgattc tacagttatc aataaagatg gtgtaacgat tgctaagaaa
     2521 gatcctaatg atgcaactaa atctaatgtt accttaacag aggatggctt aagcaacggt
     2581 ggtaaccaaa tcaccaatgt tgatagcggc ttaaaaggta aagatggtca acctgttaaa
     2641 cttactgagg cagcaggtga tgttcttaac aatgcggtta acgtcggtga cttgaaaaat
     2701 actgttactg agactgttac tgaggtgaca acagatgcgt ttggtctaaa agatgagaac
     2761 aacaaggagt ttaagcaaaa cttaggtacg acagctaaga tcattggtga tggcaatgtt
     2821 aaaacaaacg ttgttgagaa agatggtggc catgcattag aagttagcct agtgaataat
     2881 attactgttg gtaaagatgg tgaaccaggt aaagttggta ttgctggtaa agacggtaaa
     2941 gatgctattg ctttagatgg taaagatgga gctatcactg taggtcgccc tggcaaagat
     3001 ggtattgatg gtactatcgg tgtaaacggt aaagacggtt ctgcagttgt tcttaacggt
     3061 aaagatggtt caatcggctt gaacggtaca aatggtaaag atggtttaac cattaaaggt
     3121 actgagggca aaccagggct tgttggtaaa gatggtttac caggtaaaga tggtgtaact
     3181 cgtatccaat atcagccaac agatggcaat ggtgatccta taggtgaacc agagcaagta
     3241 gctactttaa atgatggttt acactttaaa ggtgatgatg caacactagt atctaagaaa
     3301 ttgaatgaaa ccttagaaat cattggtgga gctgataaag ataagctaac tgcaaataac
     3361 atcggcgtaa acagcaaaga tggtaaatta tctgttcaat tagctaaaga tcttaaagac
     3421 ttgaactcag ctacattcaa accacaagat ggagagaatg gtcaagaagg taaaggtcca
     3481 acaacagtag taaataataa tggcgtaact atcacgccaa aagatggcac agatggtaaa
     3541 gcagttaagc ttactgaaga cggtctagac aatggcggca accaaattgt taacgtgaag
     3601 agcggtattg acggtttagc aggtaaagac ggcattaacg gtaaagatgg taagccaatc
     3661 acttcagtga acgacttaac agacgatcaa ttaaaagaag ttggtactaa cgctgcgaat
     3721 attaatgatg ttgtaaatgc aagcaacgat attatcgaga aaggctttag cattgcagcg
     3781 gataatggtg aaactgatca cgttaaacta ggtgaaactg ttaagtacac ctctaaagac
     3841 aaaaacatca tcacaacagt aagtgataat gtgattgact ttacgttaaa taacgatttt
     3901 accattggcg gtccaggtaa agatggttca atcggtgtta aaggtgcaga tggtacagtt
     3961 ggtgttactt taaacggtaa agatggctct atcggcttaa ccggtccgaa aggcgctgac
     4021 ggtaaagatg gtgcaagtgc aactatctct gtaaaagacg gtgctaaagg ccttgacggt
     4081 aacgacggta aagatggcga aagcaaaacc cgtatcgttt atgagaaacc agacggtaca
     4141 actgaggaag ttgcaacact gaacgatggc ttaagattcg taggtgatga cggtaaagaa
     4201 atcgttaaga aacttaatga gacgttaaaa atctcaggcg gtgatactga tttaacgaac
     4261 ctgtccgata acaatatcgg tgttgtaaac aacaatggtg cgttaacagt taaattagcg
     4321 aaagacattg atttaacgaa agatggttct gtaactattg gtgatactac ggtaaataat
     4381 gatggtttaa ctatcaaaga tggcccaagt gtaactaaag ccggtatcaa tgcaggcgat
     4441 aagaaaatca ttaacgttca agacggtgaa atttctgcaa ccagcaaaga tgcggtaaat
     4501 ggtagccagt tatatcaagt gcaagaagtt gctaacgcag gttggaactt aacagcaaat
     4561 ggtaaagaca aaggcaacgt taaaccagga gcaacagttg atctaaacaa caaagacggt
     4621 aacattatta ttactaaaga aggtaataac gtgaccttcg gcttgaaaaa caacttaacc
     4681 attggtggta aagatggtaa agacggtcaa atcggtattg ctggcaaaga cggtaaagat
     4741 ggcgtaacca ttaaaggtga cggcacaatt actgcaggcc gtgacggtaa agacggtgtt
     4801 gatggttcaa ttggagctac aggtaaagat ggcgcttctg ttgtattaaa cggtaaagat
     4861 ggctctatcg gcttaaccgg tccgaaaggc gctgacggta aagatggtgc aagtgcaaat
     4921 attgcagtga aagatggtgc tgaaggtgtt gatggtacaa atggtaaaga tggattaccg
     4981 ggtgagaacg gtaagactcg tatcgtttat gagaccaaag ataaagacgg taaaactgtc
     5041 acagaacaag ttgcaacctt aaatgatggt ttaatcttct ctggtaacaa tgaagatgtg
     5101 aagaaccgtc agaaactgaa tactgaagtt aaagtgaaag gtgaaggtgt ggataaaaca
     5161 gcttctgaaa actttaagtc agcatcaggc aacatcaatg tgaaagcgaa tggtacagac
     5221 acattagagg tacaattagc gaaagcaatt gatttaacgg aagatggttc tgtaacaact
     5281 ggcgacacaa ccgtaaataa caatggttta accattaaag acggcccaag tgtaactaaa
     5341 ggcggtattg atgcgaaagg cacgaagatc actaacgtta aagatggtga tgtaacggca
     5401 acaagtaaag atgttgtgag cggtagccag ttgtataatg caatgcagaa tacaggctgg
     5461 tatctcactg ttgatggtaa tgtagctgaa acttcagaga aaggtgctca acgtgttaat
     5521 aacaatggta aagtggccgt ttcaggtggc aaaaacatcg ttgtttcacg taaaggttca
     5581 actgttgaag tggcaacctc aagcaatctt gaatttgatt cagtgaaagt tggtggtaca
     5641 actatcagtt caactgttgc aaaagatggc gtaaatgagc ttaacattgc aggtagcaag
     5701 aatgcgcaaa cacgtattac gaacgttgct ccgggtgtga aaggcactga tgctgttaat
     5761 gtgaatcagt tgaaaggtgc agaaaatcgt ctgaacaatc gtatcagtaa cgtagataaa
     5821 gacttacgtg caggtattgc aggtgcatta gccgcaggta atttatatca cgtaacacaa
     5881 cctggtaaat cgatggtttc agccggtgta ggtgcttacc gtggtcaagg agctattgct
     5941 gtaggttatt ctcgtttatc agataatggt aaagtgggta tcaaattcag tgttaactct
     6001 aacagccgtg gtgatacagg tgctgctgca agcgtaggtt atcagtggta gtaactgctt
     6061 gattaagctt ttcttcccca cttttgggga agaataaata aaatccttca aatagaacgg
     6121 ggaaatctgg tttatttgag ggattttttt attggctaaa ttggatttaa tggataaaac
     6181 aagcggtcaa ttctttacac ttttttgcaa aaaaacgtaa agaattgacc gcttgcataa
     6241 tttaaatata acttactcag aagtgagatt aattaccccc attttgccag ctcaatatat
     6301 tcggctagat ccggaaattg tttcacccat ttgttccatt gcacttcgtt aatttcaata
     6361 gagataatcc gttcaccgaa ctcattaaaa tgctcggttt taatgcagtt ttctgcttta
     6421 aattgtgcgt aaattgcacc ggcagtaacc gggaggagca agttttcaca cactaagtta
     6481 ctgcgaagcc tttcttgaat ggcttggaat aacagctcaa tgccgctatt ttcttgggcg
     6541 gaaagataca cggcaactgg cgtgccatcg gcattgcgtt caatatgcgg ttctacacct
     6601 tctaatttat caattttatt gaataccaat aaagtaggaa tatctaacgc tccgatttcg
     6661 tccaacactt gatttaccgc atcaatattt tcgtttttgc gttcgtctga gccgtcaatg
     6721 acgtggagca gtaaagtagc ttcggtggtt tcttgcaggg tagatttaaa ggctgaaact
     6781 aaatcgtgcg gtagaaaacg gataaaacct accgtatcgg caagaattgt cgtgccgaca
     6841 tcttgcacct gaatccggcg taaagtcggg tcaagggtcg caaaaagttg atcggcagca
     6901 taaacactag cttgggtaat cgcattaaac agggtagatt tgccggcatt ggtatagccc
     6961 actaaggata ctgttggaat atcggctttt tgtctggttt ttcgattttg attgcgttgc
     7021 ttgctgactt tttccaagcg gttgagtaac tgttggattc gcactttaat caaacggcga
     7081 tcggtttcga gctgtgtttc acctggacca cgcaagccaa ctgctccgcc tttttgttgg
     7141 tcttgattag taactcggcg aaccaaacgg gtcgataaat ggcgaagttg tgccagctcg
     7201 acttgtagtt tgccttcgtg cgatcttgcc cgttgagcga aaatatccaa aatcaagccg
     7261 gttctatcca ccacacggca gccgcataac gattgcaaat tacgggtctg agccgggcta
     7321 agttgatggt tcaccagcac tactgttgcc ccgagtcgtt ctaccgcttc ggcaattccc
     7381 tccgctttgc cttgccccac ataatatttg atatgcgaac tttggcggct ggtggtaagc
     7441 gttgccagca cttcgacacc ggcagattct gccagcgttt taaattccag caaattttca
     7501 acatctttat gttgcccgag atagagatgc accaaaatgg ctttatcttg attttctgga
     7561 tcggtaggcg aggcggcttc tgtagtttgg cttgcttcag ataaagcaaa gccaaacgca
     7621 gaattttctt ctgagtttgg caatgattcg tttaacattg gttgagtaat aatgttatta
     7681 ttccgcttgt tcagttactt cggcagcagg ctgagcgtgt tgggcttggt tgttattgtt
     7741 atgcgataac gcacgggcag gaacaaccgt tgaaatagcg tgtttataca ccatttggct
     7801 tacagtgttt tttaataaaa tcacaaattg gtcgaacgac tcgatctgac cttgtaattt
     7861 aatgccgtta actaaataaa ttgaaacagg aatacgctca cgacgaagtg cattcaaata
     7921 tggatcttgt aaagattgac cttttgccat tgttattttc ctttttatag ttgtgtgatt
     7981 tttaagacac caaataatgc gataagtgcc aaaggcgttt agatattatc acaattcttt
     8041 aaataatgat cgatcttttc caccattttt gctttagaac tggtcggagc taaactatct
     8101 aaccaagtca gttcgccttg ccagccacgc agccaagtaa tctgccgttt tgccagctgg
     8161 cgggtggcac aaatgccttt aaagatcgcc tcatcgagcg aaatatcgcc ttgcaaatat
     8221 tcccacattt ggcggtagcc cacacagcga atcgagggca aattaatatg caaatctttg
     8281 cgtaaaaata accgcttgac ctcctcttca aagcctagct caatcatttt gtgaaagcgt
     8341 tgttcaatcc gttggtgtag aactgcacga tcttgaggag cgattgcaaa ttgtaagata
     8401 ttataaggta gagcctcacc ttgctgagca gtgagttcgg tcatcgtttt gccggtaatg
     8461 taaaaaactt ctaacgctcg gttgatccgt tggctgtcat ttgggttaat tcttgcaccg
     8521 gcaatcgggt cgattttcac taattcttga tgcaatgttg cccagccgtg ttgtgccgct
     8581 cggctttcga tttcagcccg aatggctgga tcggcagagg gcaggggaga taacccttca
     8641 agcaatgcct tgtaataaag cattgtgcca cccaccaaga gcggaatttt accctgtgag
     8701 gtaatatctg ccatttcacg caacgcatca gcgtggaaat tcatcgccga atagctctcg
     8761 gcagggtcta aaatatcaat caagcgatgc ggtgcaaggg caagctcttc cttgcttggc
     8821 tttgccgtgc caatatccat tcctttgtaa attaaggcag aatcaacact gattacctca
     8881 accggcaatt cttggcgtaa agcaatagct aaatcggttt tcccggaggc agtcggcccc
     8941 attaaaaaaa gggcaagggg ttttttattc atattaatat actgtatata gatacaaaaa
     9001 aagccgtgaa attcacagct tttttgttac aatcctgatt gtgctactgg taggctcgtt
     9061 agggctattc gcagtagcct gagcctaaat catccttagt agcactaagg aggcttacaa
     9121 tgaaagaact actattgatt ttagttgcac ttgggtttct gtgtgcagca gttgaaattt
     9181 tcacgctctt tcaactaatc tatctgttct tgtaagcg
//
LOCUS       DANLYI010000089         9101 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.089, whole genome shotgun sequence.
ACCESSION   DANLYI010000089 DANLYI010000000
VERSION     DANLYI010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 9101)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9101)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9101
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.089"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            197..943
                     /gene="znuC"
                     /locus_tag="PXD81_001472"
     CDS             197..943
                     /gene="znuC"
                     /locus_tag="PXD81_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005713848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HDL5537491.1"
                     /translation="MVSLENIEVSFNGQKALQNINLSIYPNSITTIVGPNGGGKSTLL
                     KILLKLLSPNKGKVIHQKGLKIGYVPQKLHLEPSMPITVYRFLALKPHTTKEMINQAL
                     DLFSITHLTENSMQKLSGGELQRVLLARAILDRPQLLVLDEPMQGVDIIGQTELYQLL
                     NKTRDLLNCAILMVSHDLNIVMANTDEVLCINKHICCAGSPEVITHDPSFIHFFGDQF
                     ARNVAFYSHKHNHHHNLHGDICLGECSCEH"
     gene            1108..3921
                     /gene="sucA"
                     /locus_tag="PXD81_001473"
     CDS             1108..3921
                     /gene="sucA"
                     /locus_tag="PXD81_001473"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HDL5537492.1"
                     /translation="MQYTHFDDWLASSALGGANQSYIEEIYEQYLENPANVDESWRNI
                     FDSFPKTPQVEQAHTPIRDYFRKLAREQQPQSVTIIDPEAGAKQVRLLQWINAHRFRG
                     YLEAKLDPINYYRWKTSSVPELDYKYHGFTDEDLNETFTVGRYVYDKDTMKLSELDKA
                     LKETYLGPIGLEFMHVQDIEQRNWLQAKMESVLNKPLFTKAEKLNLLSELTAADGLER
                     YLGAKFPGAKRFSLEGSDAFIPMMKEIIRHASRQGMTDVVLGMAHRGRLNMLVNVFGK
                     RPAELFDEFAGKHADESRTGDVKYHQGFSSNFAVDDKNVHLTLAFNPSHLEIVSPVVI
                     GSVRARQERMRDIEHEKVLAVTVHGDSAVAGQGVVQETLNMANVRGYKVGGTIRIVIN
                     NQIGFTTSNPNDTRSTEFCTDIAKMIQAPIIHVNGDDPEAVAFAARMAVEYRTKFKRD
                     IFIDLISYRRHGHNEADEPLATQPMMYSIIKKHPTPRKVYADRLITEGVLTNEQEIEM
                     LNLYRDALDNGECVVPEWREMDMAAVDWLQYLNYDWTALYESKFPEERFLTIAKRVCE
                     YPASLKAHSRVEKIYTDRKEMYEGKKLFDWGMAETMAYATLLDEGTHVRLSGEDAGRG
                     TFFHRHAVIHNQNDGTGYVPLTQLHANQGRFQVWDSVLSEEAVLAFEYGFATTDPKTL
                     TIWEAQFGDFANGAQIVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARVERY
                     LQLCAQQNIQVCVPSTPAQVYHMLRRQAIRKMRRPLVAITPKSLLRHPLAVSSLDELI
                     NGSFQNVIGELDNIDPKQVKRVVMCSGKVYYDLIEKRRENNQTDVAIIRIEQLYPFPL
                     EEMQQILEPYAHVKDFVWCQEEPLNQGAWYCSKHNFEASLPEDAKLTYAGRPASASPA
                     VGYPSLHAKQQKQLVEDALTIAE"
     gene            4024..5253
                     /gene="odhB"
                     /locus_tag="PXD81_001474"
     CDS             4024..5253
                     /gene="odhB"
                     /locus_tag="PXD81_001474"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005654196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HDL5537493.1"
                     /translation="MTIEILTPDLPESVADATVATWHKKVGDNVKRDEILVEIETDKV
                     VLEVPASSDGILAEITQEQGATVVSKQSLGKLVVAKAGDISSATIEQKTESTPSDRKH
                     AAIENSHANADDQGPAIRRLLAEHDLQAADIQGSGVGGRITREDIEKEIAKRVQINQV
                     KGSATSQNTVSTVAFNSRSEKRVPMTRLRKRIAERLLEAKNTTAMLTTFNELDMQPIM
                     NLRKTYGEKFEKQHGVRLGFMSFYIKAVVEALKRYPEINASIDGDDVVYHNYFDISIA
                     VSTPRGLVTPVLRDCDKMSMADIEKKIKELAEKGRDGKLTVEDLTGGNFTITNGGVFG
                     SLMSTPIINPPQSAILGMHAIKDRPVAIDGQVVIRPMMYLALSYDHRLIDGKESVGFL
                     VAVKELLEDPTRLLLEI"
     gene            5546..6706
                     /gene="sucC"
                     /locus_tag="PXD81_001475"
     CDS             5546..6706
                     /gene="sucC"
                     /locus_tag="PXD81_001475"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HDL5537494.1"
                     /translation="MNLHEYQAKQIFKQYGLPVSQGIACKTAEEAVEAIKQLQGEQWA
                     IKCQVHAGGRGKAGGVKLVRNEAEVREFADKWLGKRLVTFQTDANGQPVNTLYLEETC
                     NIDRELYLGAVVDRSAQKVIFMASSAGGMNIEEVAEKTPELIHKAVIDPLTGGQGYQG
                     RELAFKLGLKGDEVKQFADIFVKLANLFVEKDLALLEVNPLVITKEGQLLCLDAKMSI
                     DSNALYRHPELKELQDPSQDDEREAEAEKWNLNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLYGGKPANFLDVGGGATKERVAAAFKLILSDPNVKAVLVNIFGGIVRCDLIADGVIA
                     AVNEVGVNVPVIVRLEGTNAAQGREILANSGLSILAASSLKQAAELAVKSAN"
     gene            6715..7584
                     /gene="sucD"
                     /locus_tag="PXD81_001476"
     CDS             6715..7584
                     /gene="sucD"
                     /locus_tag="PXD81_001476"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HDL5537495.1"
                     /translation="MSILIDKNTKVICQGFTGGQGTFHSEQALAYGTQLVGGVSPNKG
                     GTTHLGLPVFNTVREAVEATGATATVIYVPAPGCKDAILEAIDAGIQLIVCITEGIPT
                     LDMLQVKQRLNQTGVRMIGPNCPGVITPDECKIGIMPGNIHKKGNIGIVSRSGTLTYE
                     AVKQTTDEGFGQSTCVGIGGDPIPGSNFIDILKLFQDDPETEAIVMIGEIGGSAEEEA
                     AAFIKENVTKPVVSYIAGVTAPKGKRMGHAGAIISGGKGTADEKFKILEDAGVTTVRS
                     LAEIGSALRKILG"
     gene            complement(7655..9100)
                     /locus_tag="PXD81_001477"
     CDS             complement(7655..9100)
                     /locus_tag="PXD81_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine:cation symporter family protein"
                     /protein_id="HDL5537496.1"
                     /translation="MDSIILFFESVLTWIVNTIDGPLWDVTIILLLGAGLFFTLTTGF
                     VQLRLLPQSLREMWGGRSTEGKSLTPFQAFTTGLASRVGVGNIGGVATAIALGGPGAV
                     FWMWITALMGMSSAFAESSLAQLYKTKDPNGMFRGGPAYYITRGLKLPSLAIAFAVTL
                     IFTFGFAFNAVQSNSIVEATKNAWDWNPVYVGMTLVVITGLIISGGVKRIGKVSAQVV
                     PMMALFYLIIAVIILGMNIEKVPSIISLIIESAFDFSAAAGGTFGALVSKTMLMGIKR
                     GLFSNEAGMGSAPNSAATSDAKHPASQGLIQMLGVFVDTIVVCTCTAVIILMSNNYGG
                     ETLRGISLTQSALQFHLGEFGLHFLAFVILLFCFTSIIGNYAYAETNIRYIKNSPWFV
                     WGFRAIVLFFVYFGAVRDGGIVWAFADTVMASMAVINLVAILILAPIVWIILKDYLRQ
                     VKSGKEPVFRIEEHQDLIHKGVDPLIWKSKE"
BASE COUNT         2858 a         1962 c         1897 g         2384 t
ORIGIN      
        1 taaaatttac atatttttct gagtagacta ttttaccaag aaaaaattca tttaatctat
       61 tgtcacaaca cgaaaaatca gctaatttta actcaatacc cctaattttt ttatatcaat
      121 gaaaatccat catcttaaac cgaatctaaa taccactaca tttgaactat tcaaacagaa
      181 tttatccgcg acatcactgg tttctcttga gaatattgaa gtgtctttca atggacaaaa
      241 agcattacaa aatattaatt tatctattta tcctaactct atcacgacga tagtcgggcc
      301 aaatggtgga ggaaaatcta ccctgcttaa aattttgcta aaattactta gcccaaataa
      361 aggaaaggtt attcatcaaa aaggactcaa aattggctat gtaccacaaa aattgcattt
      421 agaaccctcg atgcccatta ctgtttatcg atttcttgcg ctaaaaccac atactaccaa
      481 agaaatgatt aaccaagcac tagatctgtt ttctattacc catctcactg aaaatagtat
      541 gcaaaaatta tcgggtggtg aacttcaacg tgtattgctg gctcgggcaa ttttagatcg
      601 tccgcaactt ttagtgttag atgaaccaat gcaaggcgtt gatattatag gccaaaccga
      661 actctatcaa ttacttaata aaacaaggga tctccttaac tgtgctattt taatggtctc
      721 acacgactta aatattgtga tggcgaatac agatgaagtg ctttgcatca ataaacatat
      781 ttgttgtgcc ggaagccccg aagtcattac acacgatccg agctttatcc atttttttgg
      841 cgaccaattt gcacgtaatg ttgcatttta ttcacataaa cataaccatc accataattt
      901 acatggcgat atctgccttg gtgaatgtag ttgtgagcac tgataggcgt cctttttacc
      961 aaatgtttaa aatttgtaaa aataatgcta aaaataaccg cttgcagcac aagatttatc
     1021 taaaaatcag acttacttca cattttattt gcttaaattg gagtataata agggcgtgat
     1081 ttcataccac aaacaggtca ggtctttatg caatatacac attttgatga ttggcttgca
     1141 tcatctgcat taggcggtgc aaaccaatct tatatagaag aaatttatga acagtatcta
     1201 gaaaatccag caaatgttga tgaaagctgg agaaacatct ttgattcatt tcctaaaact
     1261 ccccaagttg aacaagctca cacccctatt cgtgactatt tccgtaaatt agcccgtgaa
     1321 cagcaacctc aatccgtcac tattattgac ccagaagcag gggcgaaaca agttcgctta
     1381 ctgcaatgga ttaacgctca ccgtttccgt ggctatttag aggcaaaact agacccaatt
     1441 aactattacc gttggaaaac ctccagcgtg cctgaattgg actacaaata tcacggcttc
     1501 accgatgaag atctcaacga aacttttacg gtcggtcgct atgtctatga taaagacaca
     1561 atgaagctca gcgaactgga taaagcccta aaagaaactt atttagggcc tattggttta
     1621 gaatttatgc acgttcaaga tattgaacaa cgcaactggc ttcaagccaa aatggaaagc
     1681 gtgctaaaca aaccgctgtt caccaaagca gaaaaactga atttattaag cgaattgacc
     1741 gctgccgatg gtttagagcg ttacttaggg gcaaaattcc cgggggcaaa acggttctca
     1801 ttggaaggta gtgatgcctt tattccgatg atgaaagaaa ttatccgcca cgcttcccgt
     1861 caaggtatga ccgatgtggt tttaggtatg gcacaccgtg gtcgtttgaa tatgttagtg
     1921 aacgtatttg gtaaacgccc ggcagaattg tttgatgaat ttgccggcaa acacgctgat
     1981 gaaagccgca cgggcgatgt gaaataccac caaggtttct cctcaaactt tgccgtagat
     2041 gataaaaacg ttcaccttac ccttgctttc aacccatcgc atttagagat tgttagcccg
     2101 gtggtaatcg gctcagtgcg tgcaagacaa gaacgtatgc gtgatatcga acacgaaaag
     2161 gtgcttgcgg tgaccgttca cggtgactct gcagtggcag ggcaaggtgt agtgcaagaa
     2221 accttaaata tggcaaatgt tcgtggctat aaagtaggag gcactatccg tattgtgatc
     2281 aacaaccaaa tcggatttac cacctctaac ccaaatgaca cccgctccac cgaattttgt
     2341 accgacattg ccaaaatgat tcaggcaccg attattcacg ttaatggtga tgatccggaa
     2401 gctgttgcct ttgcagcgag aatggctgtg gaatatcgta ctaaatttaa acgtgatatt
     2461 tttattgatt taatctctta ccgtcgtcac ggtcataatg aagccgatga gcctttagca
     2521 actcagccaa tgatgtatag catcatcaaa aaacacccga cacctcgcaa agtctatgcg
     2581 gatcgtttaa ttaccgaagg tgttttaacc aacgagcaag aaatcgagat gttaaacctg
     2641 tatcgtgatg ccttagacaa cggcgaatgc gttgtgccgg aatggcgtga aatggatatg
     2701 gcagcggtcg attggctaca atatctcaat tacgactgga ctgccctata cgaaagtaaa
     2761 ttccctgaag aacgattctt aaccattgcc aaacgggtgt gcgaatatcc ggcttcgctt
     2821 aaagctcatt ctcgggttga aaagatctac actgatcgca aagagatgta tgaaggcaag
     2881 aaattattcg actggggtat ggctgaaaca atggcatacg ctactttgtt agacgaaggc
     2941 acacatgttc gcctttcagg agaagatgcc ggacgtggca ctttcttcca tcgccatgcg
     3001 gtaatccata accaaaatga tggaacaggc tatgtgccac ttactcaact gcacgccaat
     3061 caagggcgtt tccaagtatg ggattccgta ctctctgaag aagcggtact cgcctttgaa
     3121 tatggctttg ccactaccga cccgaaaaca ctgaccattt gggaagctca atttggagat
     3181 ttcgctaacg gagctcaaat tgttatcgac caatttatca gctcaggcga acaaaaatgg
     3241 ggcagaatgt gcggtttagt gatgttatta ccacacggct atgaaggtca agggccggaa
     3301 cactcctctg cccgagtgga acgctattta cagctttgtg ctcagcaaaa tattcaagtt
     3361 tgcgtgccat caacaccggc acaggtttac catatgttac gccgtcaagc gatccgcaaa
     3421 atgcgtcgcc cattggttgc aatcacacca aaatcattgc tacgccaccc acttgcggtg
     3481 tcatctcttg atgaattgat taacggttca ttccaaaatg tgattggcga gctcgataat
     3541 atcgatccta aacaagtcaa acgtgtggtg atgtgttcgg gtaaagtgta ctacgactta
     3601 attgaaaaac gccgtgaaaa taaccaaacg gacgttgcca ttattcgtat cgaacagctc
     3661 tacccgttcc cacttgaaga aatgcaacaa attctcgagc cttacgctca cgttaaagac
     3721 tttgtctggt gtcaagaaga gccactaaac caaggtgcgt ggtattgcag caagcacaat
     3781 tttgaggctt ctctgccgga agatgcaaaa ttaacttacg caggcagacc ggcttctgct
     3841 tcaccggcgg tgggttatcc atcgttacac gcaaaacaac aaaaacaact tgttgaagat
     3901 gctttaacga tcgctgaata ataaaatggg cgtatatacg ccctacaagc ggttcacttt
     3961 taaccaaatt ttacaattta cgctgtcgga gttttaaaac tctctcgaaa aaaggaataa
     4021 gttatgacca ttgaaatttt aacccccgat ttacccgaat ctgttgccga tgcgaccgtt
     4081 gccacttggc ataaaaaagt gggcgacaat gtaaaacgtg atgaaatttt agtcgaaatt
     4141 gaaacagaca aagtcgtttt agaagtaccg gcttcaagcg atggtattct tgcagaaatt
     4201 acccaagaac aaggtgctac tgttgtatcc aagcaatctc tgggtaaatt agtagtagct
     4261 aaagccggtg atattagctc tgcaactatt gagcaaaaaa cagaatccac gccatcagac
     4321 cgcaaacacg cagcaattga aaacagccac gcgaacgctg atgatcaagg gcctgcgatt
     4381 cgccgtttat tagccgaaca cgacttacaa gccgctgata ttcaaggcag tggtgtaggc
     4441 ggcagaatca cccgcgaaga tattgaaaaa gagattgcca aacgagtgca aatcaaccaa
     4501 gtaaaagggt ctgcaaccag ccaaaacacc gttagcactg tagcatttaa cagccgtagc
     4561 gaaaaacgtg tgccgatgac acgactccgc aaacgtattg ccgaacgctt actagaggca
     4621 aaaaacacca ctgcaatgct cactactttt aacgagctgg atatgcaacc aattatgaat
     4681 ttacgcaaaa cctatggcga aaaattcgaa aaacaacacg gtgtgcgttt aggctttatg
     4741 tcgttctaca ttaaagccgt ggttgaagca ttaaaacgct accctgaaat caatgcctcc
     4801 attgatggcg atgatgtggt ttatcataac tatttcgaca tcagcattgc ggtttctacc
     4861 ccacgaggct tagtcacacc tgtgttgcgt gattgcgaca aaatgagtat ggcagatatt
     4921 gagaagaaaa ttaaagaact tgccgaaaaa gggcgtgacg gcaaattaac cgttgaagac
     4981 ttaaccggcg gtaactttac catcaccaac ggcggggtat tcggttcatt aatgtccacc
     5041 ccaattatca acccgccaca aagtgcgatt ttgggtatgc acgctattaa agaccgccct
     5101 gtcgccattg acggacaagt agtgatccgc ccgatgatgt acctagcctt aagctatgac
     5161 caccgtttaa ttgacggtaa agaatctgtc ggtttcttag tggcagttaa agagttatta
     5221 gaagacccaa cccgtcttct tttggaaatt taatcgaaaa tatgaccgct tgtactttta
     5281 gctacaagcg gtctgttttt aataaaattt gacaaaatcc gataaacgct ctatttgagt
     5341 tcccttctga gccacctgag cgactttgaa tttgtaggaa aaatttagtg tttgagctta
     5401 caaggcaagc gagttataaa tttttccgtc aagcaaattc aaactaagcg aagaaagtgg
     5461 cgaagcaggg tgtgctttct tttggttact tttctttgca caagcaaaga aaagtaacat
     5521 aaaaaaaacc aaacgaggaa actctatgaa tctacacgaa tatcaagcaa aacaaatatt
     5581 caaacaatac gggcttcccg taagccaagg gattgcttgc aaaacggcag aggaagccgt
     5641 tgaggcaatt aaacaactac aaggtgaaca atgggcgata aagtgccaag ttcacgctgg
     5701 tgggcgtggc aaagccggcg gggtgaaact ggttcgcaat gaagccgaag tgcgtgaatt
     5761 tgccgataaa tggctcggca aacgcttagt taccttccaa accgatgcta atgggcaacc
     5821 ggttaatacc ttatatttag aagaaacctg caatatcgac cgtgaattgt acttaggtgc
     5881 agtggttgat cgctccgctc aaaaagtgat ttttatggca tcaagtgccg gtggtatgaa
     5941 tattgaagaa gtagcggaga aaacccctga attaattcac aaagcggtaa ttgacccact
     6001 caccggtggg cagggttatc aagggcgtga attagcattt aaattaggct tgaaaggtga
     6061 tgaagttaaa caatttgcgg atattttcgt gaaactggct aacctatttg ttgaaaaaga
     6121 tttagcttta ttagaggtaa atccacttgt catcactaaa gaagggcaac tactctgctt
     6181 agatgcaaaa atgtcgattg acagcaatgc tctctaccgc cacccagaac ttaaagagtt
     6241 gcaagaccca tcgcaagatg acgaacgtga agctgaagcg gaaaaatgga atctcaacta
     6301 cgttgcacta gatggcaata tcggctgtat ggtaaacggt gcggggctgg caatggggac
     6361 gatggatatc gtcaaacttt atggtggtaa accggctaac ttcttagacg ttggcggtgg
     6421 tgcaaccaaa gagcgtgtag cagcagcttt taaattgatt ttatctgatc cgaatgtgaa
     6481 agccgtgtta gtcaatattt ttggtggtat cgtccgctgc gatttgattg ctgatggcgt
     6541 cattgctgct gtaaacgaag tcggcgtgaa tgtgcctgtt attgtgcgtt tagaaggcac
     6601 taatgccgca caggggcgtg agattttagc caatagcggg ctgagtattc tagcggcatc
     6661 atcattaaaa caagcggctg aattagcggt aaaatctgca aattaggagg aaaaatgtcg
     6721 attttaattg ataaaaacac aaaagtaatt tgccaaggtt tcaccggcgg acaaggtact
     6781 ttccacagcg aacaagccct tgcttatggc acacaattag tcggcggagt ttcgccgaat
     6841 aaaggtggca caacccattt aggcttaccg gttttcaaca ccgtacgcga agcggtagaa
     6901 gcaaccggtg caactgcaac cgttatctac gttccggctc ccggctgtaa agatgctatt
     6961 ttagaagcca ttgatgccgg catccaacta attgtctgta ttactgaagg cattccaacc
     7021 ttggatatgc tacaagttaa acaacgtcta aaccaaaccg gcgtgcgaat gatcggtccg
     7081 aactgcccgg gtgtgattac ccctgatgag tgcaaaatcg gcattatgcc gggcaatatc
     7141 cacaaaaaag gcaatatcgg tattgtttct cgttcgggta ctttaaccta tgaagcagtt
     7201 aaacaaacaa ctgacgaagg ctttggtcaa tctacttgtg tcggtatcgg tggtgatccg
     7261 attccgggat ctaactttat tgacatttta aaactgttcc aagacgatcc tgaaactgaa
     7321 gcaattgtga tgattggtga aatcggcggc tctgccgaag aagaagcggc agctttcatc
     7381 aaagaaaatg tcaccaagcc tgtagtcagc tatattgccg gtgtcaccgc accaaaaggc
     7441 aaacgaatgg gacacgccgg tgcgattatc agcggcggca aaggaaccgc cgatgaaaaa
     7501 ttcaaaatcc ttgaagatgc cggtgtgaca accgtcagaa gtttagccga aatcggttca
     7561 gctcttagaa aaattttagg ctaaactaaa agttcaagca gtcatattta cccatttttt
     7621 tgtaaatatg accgcttgtt tttttgctat taaattactc tttcgatttc caaattaatg
     7681 ggtcaacacc tttatggata aggtcttgat gttcttcaat tctaaacact ggctctttgc
     7741 cacttttcac ttggcgaaga tagtctttta aaataatcca gacaatcgga gcaagaatta
     7801 agatagccac caagttaatt accgccattg atgccatcac tgtatcggca aatgcccata
     7861 caatccctcc gtctcttact gcaccgaagt acacaaagaa taaaacgatg gcacggaatc
     7921 cccacacaaa ccacggactg tttttaatgt agcggatatt agtttctgca taagcatagt
     7981 taccgataat ggatgtgaag caaaacagta aaatcacaaa tgctaaaaag tgtaaaccaa
     8041 attcacctaa atggaattgt aacgcacttt gagtaagtga aatacctctt aaagtttcac
     8101 caccatagtt atttgacata agaatgatca ctgcagtaca agtacagact acaattgtat
     8161 ctacaaatac gcccaacatt tgaattaagc cttgacttgc cgggtgtttc gcatcagaag
     8221 tcgctgcaga atttggtgca gatcccatac cagcttcgtt tgagaataag ccacgtttaa
     8281 tccccattag catagttttg gataccaatg ctccaaacgt tccacctgca gcagcagaaa
     8341 aatcaaaggc gctttcaata attaaagaga ttatacttgg cactttttca atattcatac
     8401 ctaaaataat tactgcaata atcaagtaga ataatgccat cattggaaca acttgggcag
     8461 aaactttacc aatacgcttg acccctccag aaataattaa ccctgtaatc accactaatg
     8521 tcatgcctac gtagactggg ttccaatccc acgcattttt ggttgcttcc acaatagagt
     8581 tagattgcac agcattaaaa gcaaaaccaa aggtgaagat aagcgtcact gcaaaagcaa
     8641 tagcaagaga aggcagtttt aaaccacgag tgatgtaata ggctggccca ccacgaaaca
     8701 ttccgtttgg atctttggtt ttatacaact gagccagtga agactcagca aaggcggaac
     8761 tcattcccat taaggcggta atccacatcc agaataccgc acccggccca cccaaagcga
     8821 ttgcagtagc taccccacca atattaccaa ccccaacacg gctggctaat ccagtagtaa
     8881 aggcttggaa cggagttaat gatttccctt ccgtagaacg tcctccccac atttcacgta
     8941 aactttgcgg aagaagacgc agctggacaa aacctgttgt aagagtgaaa aataaccctg
     9001 caccgagcaa taggataatc gttacatccc aaagtggacc gtcgattgtg ttcacaatcc
     9061 aagtcagtac actctcaaaa aatagaataa ttgaatccat a
//
LOCUS       DANLYI010000090         9095 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.090, whole genome shotgun sequence.
ACCESSION   DANLYI010000090 DANLYI010000000
VERSION     DANLYI010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 9095)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9095)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9095
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.090"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            167..883
                     /locus_tag="PXD81_001478"
     CDS             167..883
                     /locus_tag="PXD81_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein"
                     /protein_id="HDL5537497.1"
                     /translation="MKKTLLTLLFGCVVTAQAQDIKFVMEPSYPPFEMTEEKGEIIGF
                     DVDIANAICKEMNANCTFHSQPFDSLIQSLKQKQFDAAISGMGITEPRKKQVLFSEPY
                     FPSSAAFIAKKDTDFAKVKTIGVQNGTTYQHYLAKEKKEYNVKSYASYQNAILDVQNG
                     RIDAIFGDVPVLAEMARKHEGLDFVGEKINNPNYFGDGLGIATHLKNQVLVDQFNAAL
                     KTIKENGEYQKIYDKWMGGK"
     gene            complement(953..1831)
                     /locus_tag="PXD81_001479"
     CDS             complement(953..1831)
                     /locus_tag="PXD81_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF535 domain-containing protein"
                     /protein_id="HDL5537498.1"
                     /translation="MKNLYKWPAAQSLYPNQGKKSYAGKRFRYRLRSWLHHSKIKQFE
                     QFVTQNPQLIPLLNARPNYSYPVAHRFLDKRFSAKQRLQKITDNLLFLPQKLAHLPPL
                     WEQAVNFGEIIADFELWLNINEHQPMEGFWALELRHRPTNQLVYLLTFGKLDEALLIA
                     VIQGPNFEGSKELVKQLTKSCHGLRPAYLMVETMKALTAVLGYQSLLGIPQKYQNKSR
                     WVQSKRYVVDYDAIFAESGGKPGNYWQLPLELESKKIDEIPSKKRSMYRKRYEMLANL
                     KQTMELMLKTYTGLNG"
     gene            complement(1907..3232)
                     /gene="hslU"
                     /locus_tag="PXD81_001480"
     CDS             complement(1907..3232)
                     /gene="hslU"
                     /locus_tag="PXD81_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011609688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HDL5537499.1"
                     /translation="MMSMTPREIVSELDAHIIGQKDAKRAVAIALRNRWRRMQLPEDL
                     RQEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LADVSMKLVRQQAVEKNKMRAQDAAEDRILDVLLPPAKDQWGNVQESDNNSTRQTFRK
                     KLREGQLDDKEIEIDVAAQVSVEIMTPPGMEEMTSQLQSLFEGMSPSKTKKRKMKIKD
                     ALKVMLDEEAAKLVNPEELKQQAIEAVEQHGIVFIDEIDKICKKGEHSGGDVSREGVQ
                     RDLLPIIEGSTVNTKHGMVKTDHILFICSGAFQVARPSDLLPELQGRLPIRVELKSLT
                     KEDFERILTEPNASLTLQYRELMKTEGVEIEFTKDGISRIAESAFRVNEKTENIGARR
                     LHTVLERLMDGISFDASERRGEKIIIDENYVSEALNDVVENEDLSRFIL"
     gene            complement(3489..4010)
                     /gene="hslV"
                     /locus_tag="PXD81_001481"
     CDS             complement(3489..4010)
                     /gene="hslV"
                     /locus_tag="PXD81_001481"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HDL5537500.1"
                     /translation="MTTIVCVRKDGKVAIGGDGQATLGNCIEKGTVRKVRRLYKDKVV
                     TGFAGSTADAFILRDLFEKKLELHQGHLVKSAVELAKEWRTERALRRLEAMMIVANDS
                     EFLLVSGSGDVIEPEQDVLAIGSGGNYAKAAALALLRTENNLSAKEIVAEALKIAGDI
                     DIYSNYNHVIEEV"
     gene            complement(4071..4784)
                     /gene="sfsA"
                     /locus_tag="PXD81_001482"
     CDS             complement(4071..4784)
                     /gene="sfsA"
                     /locus_tag="PXD81_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HDL5537501.1"
                     /translation="MKFPPLSHGILLRRYKRFLADIQLPNGEQITLHCPNTGAMTGCA
                     NPGDTVWFSTSHNPKRKYAHTWELTQTQAGDFICVNTLRANQLVQEALENQWISELAD
                     YQHILPEQKYGSENSRIDFLLKSDLLPDCFVEVKSTTLLTENGVGMFPDAKTERGQKH
                     LRELTKIAEQGLQAVIFFAILHTGIERFEVAKQIDPKYAELFAQAIAQKVQVLSYKMA
                     ISLENGQPIAATLTKKCEF"
     gene            complement(4800..5669)
                     /locus_tag="PXD81_001483"
     CDS             complement(4800..5669)
                     /locus_tag="PXD81_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HDL5537502.1"
                     /translation="MLYLLIATLIWASSFIVGKVAISAFDPVQIMQIRLTLAAFIALP
                     FFIPAYKKIQKNQYIKVWLISFLTFPLYVILQFSGLKLTSAASAVALLGLNPLLTVLV
                     GYFCFKKQATRLDFILSLFACLGILIMASGSDDSGSINVLGLLLVTLGSLSFIVGMYL
                     SKEILQSVKVIDLTKIMLVQGAITALPITLLFAENWQLPTSLSAWASVAYLGIICTSL
                     AILLWNKGISQSSPILAGILLALEPVFGLLMALVFLSEQLSLVTWVGIIIVICTALLS
                     VLLPMMKRYKRLI"
     gene            complement(5670..6062)
                     /locus_tag="PXD81_001484"
     CDS             complement(5670..6062)
                     /locus_tag="PXD81_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDL5537503.1"
                     /translation="MKSPILGFHHIALIVSDYEKSKHFYTRILGAEIIAETYREQRAS
                     YKLDLRFKDGSQIELFSFPETPPRFTSPETCGLRHLAFRVENIEYAIAFLEQHQLPHE
                     GIRIDEITGKRFTFFRDPDDLPLEFYEV"
     gene            6146..7291
                     /gene="mutY"
                     /locus_tag="PXD81_001485"
     CDS             6146..7291
                     /gene="mutY"
                     /locus_tag="PXD81_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005609167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HDL5537504.1"
                     /translation="MNSNSPFPLAKAKASDPFAKAVLAWFEQYGRKHLPWQQNKTLYQ
                     VWLSEVMLQQTQVATVIPYFARFMERFPTVIDLANASIDEVLHLWTGLGYYARARNLH
                     KAAIQIRDEFGGEFPTRFDDVLALTGVGRSTAGAILSSVLDAPHPILDGNVKRVLSRY
                     FAVEGWAGEKAVENRLWQLSESVTPDTQVADFNQAMMDLGAMICTRTKPKCLLCPLQE
                     NCKTNAMQAWDSFPAKKPKKTLPERQTYFLVLKSGSKVLLQKREAKGIWGGLFVFPQF
                     ESLEALKRSDFMQNLQISQQLTAFRHTFSHFHLDITPVLVETDLKKTSENSPLVVEEK
                     AGNYLSKVSSNADYWYDLHQHNEVGLATPVKRILDELAFSVKNEIKD"
     gene            7295..7564
                     /locus_tag="PXD81_001486"
     CDS             7295..7564
                     /locus_tag="PXD81_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015674328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HDL5537505.1"
                     /translation="MARTVFCEYLKQEAEGLDFQLYPGELGKRIFDSISKQAWSEWIK
                     KQTMIVNEKKLSMMNPEHRKLLEQEMVNFLFEGKEIIIEGYKPVE"
     gene            7581..8672
                     /gene="mltC"
                     /locus_tag="PXD81_001487"
     CDS             7581..8672
                     /gene="mltC"
                     /locus_tag="PXD81_001487"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HDL5537506.1"
                     /translation="MKKYAKYLPMLAIIPFLIACGSNKSTKKPKSTRVDYSKDTNGLD
                     ILAGQFSHNIDDIWGSNELLVASKKDYVKYTDKFYTRSHISFEEGVITIETLGNQNHL
                     RNSIIHTLLMGRDPKGIDLFASGDAPISSNPFLNGQVEDQFGRNITNVAIANDFATYL
                     LQNKVQTRRLKNGQTVSFVTIKMVAGHIEVRARQYLPLVRKMAKQYGIEPSLILGIME
                     VESAFNPYAISYANAIGLMQVVPRTAGRDIFARKGMGGQPSREYLYNPSNNIDAGTLY
                     LTILRDEYLDGIEDPVAKRYAMISAYNSGAGAVLRVFDSDRYSAIDRINDLSSDAVYR
                     ILTTAHPSSQARNYLRKVTTAREKYRNIR"
     gene            8804..8879
                     /locus_tag="PXD81_001488"
     tRNA            8804..8879
                     /locus_tag="PXD81_001488"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8837..8839,aa:Phe,seq:gaa)
     gene            8885..8960
                     /locus_tag="PXD81_001489"
     tRNA            8885..8960
                     /locus_tag="PXD81_001489"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8918..8920,aa:Asn,seq:gtt)
BASE COUNT         2637 a         1834 c         1929 g         2695 t
ORIGIN      
        1 ttttgggttg tttttttgag atagaggaat caagaggatt gagaaactac atctcaattt
       61 gatgtgtttg tatgacataa aacaaacttt atgaaaagaa gacttttttt aagaaagtaa
      121 ctgcgttaaa gtggtaatcc ttataatgaa aacacaggga ctttttatga aaaaaacact
      181 tttgaccctt ttattcgggt gtgtggttac tgcgcaggca caagatatca aatttgtgat
      241 ggagccaagc tatcctccat ttgaaatgac cgaagagaag ggagaaatta tcggttttga
      301 tgtggatatt gcaaatgcta tctgtaaaga gatgaatgca aattgtacat tccatagcca
      361 accttttgat agcttaatcc aaagcctaaa acaaaaacaa tttgatgctg caatttcagg
      421 tatgggtatt acggaaccac gtaagaaaca agtattattt agcgagcctt actttccaag
      481 ctctgctgcc ttcattgcga aaaaagatac agattttgct aaggtgaaaa cgattggtgt
      541 gcaaaatggg actacttacc aacactattt agcaaaagag aaaaaagagt acaatgtgaa
      601 atcttatgcc tcttatcaaa atgctatttt agatgtgcaa aatgggcgaa ttgatgctat
      661 ttttggcgat gtgccggtat tggctgagat ggcaagaaaa cacgaagggc tagattttgt
      721 aggggagaaa atcaataatc caaactattt tggtgatggt ttaggcattg caactcactt
      781 gaaaaaccaa gtattagttg atcaattcaa tgcagcatta aaaacgatta aagagaatgg
      841 cgaatatcaa aaaatctatg ataaatggat gggcggcaag taacttgtaa gcggtcattt
      901 tttgcaaagt tttgcaaagt tttgcagatt ttgaccgctt gtattctttg atttacccat
      961 ttaagccagt ataagtttta agcataagct ccatggtctg ctttaaatta gctagcattt
     1021 cataacgctt gcgatacatt gagcgttttt tgcttggaat ttcatctatt tttttacttt
     1081 ccaattcaag gggtaactgc caataatttc ccggctttcc gcccgattcc gcaaaaatag
     1141 catcataatc cactacatag cgcttacttt gcacccagcg ggatttattc tggtatttct
     1201 gtggaatgcc tagtaaactc tgatagccaa gaacagccgt taaagccttc atcgtttcaa
     1261 ccattaaata ggccgggcgt aagccgtggc aacttttggt taattgtttc accagctctt
     1321 ttgaaccttc aaaattcgga ccttgaatta cggctatcaa caaggcttca tctaacttac
     1381 caaatgttaa taaataaact agctgattag tcggtctgtg gcgaagttct aatgcccaaa
     1441 aaccttccat cggctgatgt tcattgatgt taagccataa ctcaaaatct gcaataattt
     1501 caccaaaatt gaccgcttgt tcccatagag gtggtaaatg agccaatttt tgcggtaaaa
     1561 acagcaggtt atctgtgatt ttttgtaagc gttgttttgc actgaaacgc ttatctaaaa
     1621 agcggtgagc cacggggtag ctgtaatttg gtcgagcatt taagagcgga atcagttgtg
     1681 gattttgtgt aacgaactgc tcaaattgct tgattttgga atgatgcaac caagagcgaa
     1741 gacggtaacg aaaacgtttc cctgcgtaag acttctttcc ctgatttgga taaagcgatt
     1801 gtgcagcagg ccatttgtat agatttttca tttttacaac taacaatatg aaaagcggct
     1861 aattcgcaag atttttcgct aaaataaccg cttgtataac ttaaaactac aaaataaagc
     1921 gacttaaatc ttcgttttcc accacgtcat ttaacgcttc ggaaacatag ttttcatcaa
     1981 tgatgatttt ttcaccacgg cgttcgctgg catcgaacga aatgccgtcc atcaaacgct
     2041 caagcacggt gtgtaaacgt cttgccccga tgttttcggt tttctcgttc acacggaatg
     2101 cactttctgc aatacggctg ataccgtctt tagtgaactc aatctccacg ccttcagttt
     2161 tcatcaactc acgatattgc aaggttaatg aggcattcgg ttcggttaaa atacgctcaa
     2221 aatcttcttt ggtaagcgat tttaactcca cacgaatcgg caagcgaccc tgtaattctg
     2281 ggagtaaatc agacgggcga gccacttgga atgcccccga gcagataaac agaatatggt
     2341 cggttttcac cataccatgc ttggtattta ccgttgagcc ttcaatgatt ggaagtaaat
     2401 cacgctgaac accttcacga gaaacatctc cacccgaatg ctcgcctttt ttacagattt
     2461 tgtcgatctc atcaataaag acaataccgt gctgttccac cgcttcaatc gcttgctgtt
     2521 tcagctcttc aggatttact aatttcgccg cttcttcatc aagcatcacc tttaacgcat
     2581 ctttgatttt cattttgcgt tttttggttt tgcttggcga cattccctca aacagcgatt
     2641 gtaattgcga ggtcatctct tccattcccg gcggggtcat aatctcaacg ctcacttgag
     2701 ctgccacgtc aatttcaatc tctttatcgt ctaattgccc ttcacgcaat tttttgcgga
     2761 aagtttggcg ggtagaatta ttgtcggatt cctgcacatt tccccactga tctttcgccg
     2821 gtggcaataa tacgtctaaa atgcgatctt ccgctgcatc ttgtgcacgc attttatttt
     2881 tttcgaccgc ttgttggcgg actaatttca tcgacacatc agctagatca cggataatcg
     2941 aatccacttc tttacccaca taccccactt cagtgaattt ggttgcctca actttgataa
     3001 aaggggcatt ggcaagcttc gccaaacggc gggcgatttc ggttttaccc acaccggtcg
     3061 gaccgatcat taaaatattt ttgggggtca cttcttggcg gagatcttcc ggcaactgca
     3121 ttcttctcca gcggttacgc aaagcaattg ctaccgcacg ttttgcatct ttttgtccga
     3181 taatgtgtgc gtctaattct gagacgattt cacggggtgt cattgacata atgttattcc
     3241 ttaaattaat taccctctcc ccttgtggga gagggacaga tttttctttg ttcagaagaa
     3301 aaatcaggga gagggtaaca agcggtcttt tttcttaaac gttttgcatt aacccctctc
     3361 tctgaaaaat atttctgaac gaaatatttt tcggtctcaa tactcgctac gctcgctcct
     3421 gtggagccgt tggcaaagcc aacgttcaaa tgtaaacatt tgtcccacaa ggggagagag
     3481 ttatttaact aaacttcttc aatcacgtga ttgtagttac tgtaaatatc aatatcgccg
     3541 gcgattttta acgcttccgc aacgatttct tttgcagata aattattttc ggtgcgtaat
     3601 aacgcaagag cagcggcttt cgcatagtta ccgcctgaac caatcgccag cacatcttgc
     3661 tccggctcaa tcacatcgcc cgaacctgaa accaataaaa actcgctgtc attggcgaca
     3721 atcatcatcg cttctaaacg gcgtaaagca cgctcggtac gccactcttt tgccagttcc
     3781 actgccgatt tcactaaatg cccttggtgt aactccaatt ttttctcgaa taaatcacgc
     3841 aaaataaagg catcagcggt cgagccggca aagcctgtca ccactttgtc tttatacaaa
     3901 cggcgaactt tccgcaccgt gcctttctcg atacaatttc caagcgttgc ctgaccatca
     3961 ccaccgatag ccacttttcc gtctttacgc acgcatacaa tcgttgtcat actaaatcct
     4021 tctttttaaa aactttggct tatatgggga tatgctgtgt gttttcaagc ttaaaactca
     4081 catttctttg ttaaggttgc cgcaataggt tgcccgtttt ccaatgaaat tgccatttta
     4141 taggagagaa cctgcacttt ttgtgcaatg gcttgagcga ataattcggc atatttgggg
     4201 tctatctgct ttgccacttc aaaccgttca atacctgtgt gcaaaatagc gaaaaaaatg
     4261 accgcttgta agccctgttc agcgattttc gtcagctccc gcaaatgttt ttgcccccgc
     4321 tcggttttgg catcggggaa cattcccaca ccgttttcgg ttaaaagggt ggtagatttc
     4381 acttccacaa aacaatccgg cagaaggtcc gatttgagca aaaaatcaat ccgactgttt
     4441 tcactgccat atttttgttc aggcagaatg tgctgataat ccgcaagctc gctaatccat
     4501 tgattttcca aggcttcttg tacaagctga ttggctctca aggtattgac acaaataaaa
     4561 tcccccgctt gggtttgggt tagttcccaa gtgtgagcat atttacgttt ggggttatga
     4621 gaggtggaaa accaaactgt atcaccgggg ttggcacagc ctgtcatcgc accggtattt
     4681 gggcaatgga gggtaatttg ttcgccattt ggcaactgaa tatcggcaag aaaacgttta
     4741 taacggcgaa gtaaaatgcc gtgggaaaga gggggaaatt tcatttgcaa aaaatcctgt
     4801 taaattaacc gcttgtatct tttcatcatc ggtagcaaaa cggagagtaa ggcggtgcaa
     4861 ataacaataa taatcccaac ccaagtcact aaactgagct gttctgataa aaagaccaat
     4921 gccattagca gtccgaatac cggttcaagt gcgagtagaa tacctgcaag aataggcgaa
     4981 gattggctga tgcctttgtt ccataacaat attgccaagc tggtacaaat aatgcctaaa
     5041 tatgctacgc tcgcccatgc tgagagactg gttggcagtt gccaattttc tgcaaagagt
     5101 aaggtaatag gtaaggcggt aattgcccct tgcaccagca tgatcttggt taaatcgatc
     5161 acttttactg attgcagaat ctctttgctg aggtacattc ccacaataaa acttaaactg
     5221 cctaaggtta caagcaataa tcctaataca ttgattgaac cgctatcatc gctacccgat
     5281 gccataatta aaatccccaa gcaggcaaat aaacttagga taaaatcgag cctggttgcc
     5341 tgttttttga aacaaaaata gccgactaac acagttaaaa gcgggtttag cccgagtagt
     5401 gccacggcac ttgctgcgga agttaatttc aagccggaaa attgtaagat cacgtacaga
     5461 ggaaaagtga gaaaactaat cagccagact ttaatgtact gatttttctg gattttttta
     5521 tacgcaggaa taaaaaacgg cagagcgata aacgccgcta acgtcagccg aatttgcata
     5581 atttggacag ggtcaaaggc tgaaatcgcc actttaccta caatgaatga actcgcccaa
     5641 atcagggttg caatcagtaa atataacatt tagacctcgt aaaactctaa cggcagatcg
     5701 tccggatctc ggaaaaacgt gaagcgtttg ccggtgatct catcaattcg gatcccttca
     5761 tgcggtaatt gatgttgttc taaaaatgcg atggcatatt caatattttc cactcgaaat
     5821 gccaaatggc gaagcccgca ggtttcgggc gaggtgaaac gaggcggcgt ttcaggaaaa
     5881 ctgaataatt caatttgtga gccatcttta aagcgtaaat ctaatttata gctggcacgt
     5941 tgctctcggt aggtttctgc gatgatctct gcccctaaaa tgcgggtgta gaaatgtttg
     6001 gatttttcgt aatcggaaac aattaatgca atgtggtgga aacctaaaat gggcgatttc
     6061 atcttttttc cttaatgtgt aaaatttttg gtaaaactga ccgcttgcag tatgataatt
     6121 tatttttcgg atttcaagat ttttaatgaa cagcaattcc ccatttcctt tagcgaaagc
     6181 aaaagcttct gatccgtttg ccaaagctgt tttagcttgg tttgagcaat atgggcggaa
     6241 acatttacct tggcagcaaa ataaaacgct gtatcaggtt tggttgtcgg aggtaatgtt
     6301 gcaacaaact caagtagcga ccgttattcc ttattttgca cgttttatgg aacgatttcc
     6361 caccgtaatt gacttagcta atgcctcgat tgatgaagta ttgcatttgt ggacagggct
     6421 gggttattac gctcgggcaa gaaatttgca taaagcggca attcaaattc gagatgagtt
     6481 tggcggtgaa tttcctaccc gatttgatga tgtgttggca ttaaccggag tgggcagaag
     6541 tactgcaggg gcgattttgt cttctgtgtt agatgcccca cacccgattt tagatggcaa
     6601 tgtcaaacgg gtgttaagcc gctattttgc agtagaaggc tgggcgggtg aaaaagcggt
     6661 ggaaaatcgc ctttggcaat taagcgaaag tgtgacgcct gatacccaag tggcagactt
     6721 taaccaagcg atgatggatt taggggcgat gatttgcacc cgtacaaagc ctaagtgctt
     6781 gctctgcccg ctacaagaaa attgtaaaac gaatgcaatg caagcgtggg atagctttcc
     6841 tgccaagaaa ccgaaaaaaa ccttgccgga gcgtcaaacc tattttttag tgctgaaaag
     6901 cggctcgaaa gtgctgttac aaaaacggga agcaaaaggc atttgggggg gcttatttgt
     6961 gtttccgcaa tttgagagct tagaggctct caagcggtct gattttatgc aaaatttgca
     7021 aatttcacag caattaactg catttaggca tacttttagc cattttcatt tggatattac
     7081 cccggtttta gtagaaaccg atttgaaaaa aacaagtgaa aattcaccgc ttgttgtcga
     7141 agaaaaagca ggaaattatc tttctaaggt atcttcaaat gcggattatt ggtatgattt
     7201 gcaccaacat aacgaagtgg gtttagcaac gccggttaaa cgtattttag atgagctggc
     7261 attcagtgtg aaaaatgaga ttaaggatta aattatggct cgtaccgtat tttgcgaata
     7321 tttaaaacaa gaggcagaag gtttagattt tcaactttat ccgggggagc tgggtaagcg
     7381 tatttttgac tcaatcagca agcaagcgtg gtctgagtgg attaaaaaac agacaatgat
     7441 cgtcaatgag aaaaaactca gtatgatgaa ccccgaacac cgtaaactgc tcgaacaaga
     7501 gatggtcaat tttttatttg aaggtaaaga gatcatcatc gaaggctata agccggtcga
     7561 ataataaagg ttaaatcaca atgaaaaaat atgctaaata tttgccgatg ttggcaatta
     7621 ttccgtttct tattgcttgt gggagcaata aatcgactaa gaagccaaaa tctacccgag
     7681 tagattattc taaagatacc aatggtttgg atattttggc agggcagttt tctcacaata
     7741 ttgatgatat ttggggaagt aacgagctgt tagttgccag taagaaagat tatgtgaaat
     7801 atacggataa gttttacacc cgtagccata tttcgtttga agaaggggtg attacgattg
     7861 agactttagg gaatcagaat catctacgca attcgattat tcatacttta ttaatgggca
     7921 gagacccgaa agggattgat ttatttgcct caggcgatgc accgattagc tctaaccctt
     7981 tcctaaacgg acaggttgaa gatcaattcg ggcgaaatat taccaatgtt gccatcgcca
     8041 atgattttgc gacttatttg ttgcaaaata aggtgcaaac acgccgtttg aaaaatggtc
     8101 aaactgtgag ctttgtaacg attaaaatgg tggcaggaca cattgaagta cgtgccagac
     8161 aatatttacc gctggtgcgt aaaatggcga agcagtatgg tattgagcca agtttgattt
     8221 tagggattat ggaagtcgaa tcagcattca acccttatgc catcagctat gccaatgcga
     8281 ttggcttaat gcaggttgta cctcgtaccg ccgggcgtga tatttttgcc cgtaaaggta
     8341 tgggagggca gccaagtcgt gaatatcttt ataatccatc aaataacatt gatgcaggta
     8401 cgctttattt gactattttg cgtgatgaat acttagatgg aattgaagat cctgtggcta
     8461 aacgctatgc aatgatttct gcatacaata gcggagcagg tgcggtatta cgagtgtttg
     8521 attcagaccg atactctgcg attgatagaa ttaacgatct ttcgtctgat gcggtttatc
     8581 gaattttaac cactgctcat ccatcttctc aagctagaaa ttatttaaga aaagtaacca
     8641 ccgccagaga gaaatatcgc aatattcgtt aatgtgtgaa ttatgctgtt ttttagccga
     8701 aaagtataaa aaaagcacat ttgagcagcg gtttttttaa atttgcaaaa aaacgtttga
     8761 cgttgcaagc tgaaatcagc ataatgcacc acgcaacgac gttgcctcga tagctcagtc
     8821 ggtagagcag gggattgaaa atccccgtgt cggtggttcg attccgcctc gaggcaccat
     8881 caattcctcc ttagttcagt cggtagaacg gtggactgtt aatccatatg tcgcaggttc
     8941 gagtcccgca ggaggagcca tattctgaag ccctgatgag taatcatcag ggttttcttt
     9001 ttgagctttc cggctaacta ctaagaaatt gctcaatttt tagtattatg cgttcaaaaa
     9061 cgctaaattt ttgagaattg tatttgacga agccc
//
LOCUS       DANLYI010000091         9072 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.091, whole genome shotgun sequence.
ACCESSION   DANLYI010000091 DANLYI010000000
VERSION     DANLYI010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 9072)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9072)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9072
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.091"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            <1..305
                     /locus_tag="PXD81_001490"
     CDS             <1..305
                     /locus_tag="PXD81_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5537507.1"
                     /translation="SNVEFAKNTENITACKVPDNLSAKNIVTDFDKMTLIGLVKINEV
                     MRALFIDEKQQLLALKENDLLNNEIEIKHINLKSITYINWKLTKDCNIPYEINIKL"
     gene            314..1627
                     /gene="pilQ"
                     /locus_tag="PXD81_001491"
     CDS             314..1627
                     /gene="pilQ"
                     /locus_tag="PXD81_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus secretin PilQ"
                     /protein_id="HDL5537508.1"
                     /translation="MWRIFIYFLCGFSLAYANTISIVLKNAPTSLIFTYLAEETGKNM
                     VLDDNIETKSTLRLENKSVDEIFKTLGKVNKLSLTQEDDIVYIHKKEEKAADLTPLPI
                     MNLQNNGQNTPLVTAPKLITKTLKLHYAKASEVIESLTKGSGTFLSENGYIHFDERSN
                     SLIVKDSAKSLKNIENLVKQLDQPTEQIAIEARIVTISSEHLQELGVRWGIFSRGAGH
                     YKFGGRLEGNGLNNVTNNLNVNFPITGGASAVLQVSSINSRVLDLELSALEQENSVEI
                     IASPRLLTTNKKPASIKQGTEIPYVMYNTKSEATDVEFKEAVLGLEVVPHLSTQNQIL
                     LDLIVTQNSPNSQSGSSGLITIDKQELNTQVFAKHGETIVLGGIFQHLTQKGEDKVPI
                     LGSIPFIKRLFSQTRDKISKRELVIFVTPYILQSNEKSSNRKKSK"
     gene            1708..2121
                     /gene="nusB"
                     /locus_tag="PXD81_001492"
     CDS             1708..2121
                     /gene="nusB"
                     /locus_tag="PXD81_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HDL5537509.1"
                     /translation="MKISPRRRARECAVQAIYSWYISQNTVEEVELAFVTDQDMKGVD
                     MPYFRKLFRGVVDNVEAIDEALRPYLDRKESDVDPIERSILRLSTYELKFEHDVPFRV
                     VINEGIEVAKVFGSDDSHKYINGILDKLAPALGRK"
     gene            2184..3164
                     /gene="thiL"
                     /locus_tag="PXD81_001493"
     CDS             2184..3164
                     /gene="thiL"
                     /locus_tag="PXD81_001493"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HDL5537510.1"
                     /translation="MGEFDIIERYFNASQRPPRKDVLLSIGDDCALTSLKPNQQLAIT
                     TDTLVCGTHFLPTISPADLAYKSVASNLSDLAAMGAEPAWISLALTLPEINHDWLAEF
                     SEHFFEILDHYNVDLIGGDTTCGPLSLTITAQGIVPQDRGLFRHNAKVGDWIYVSGTL
                     GDSAGGLQLLLNQQSEQVNWNKEQSYLIQRHLRPTPRVLLGLTIASIANAAIDISDGL
                     LSDLSHILKRSDCGAVLNLENFPLSDVLLSCYSRTEAEYFALNGGEDYELCFTVPNDN
                     KAKLDKALANIGVTYTCIGQIRSAKEGLTLLRHNQPVTLPSQRGYDHFKK"
     gene            3169..3642
                     /locus_tag="PXD81_001494"
     CDS             3169..3642
                     /locus_tag="PXD81_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005595983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HDL5537511.1"
                     /translation="MKPINLKNPIHFFAFGFGSGLLKPAPGTWGSLAGLIVAIALWSI
                     TQSALFFGLLTLVSFIAGCVICTKASQDLEVHDDGRIVWDEIVAIFLMFTCLPEYNLL
                     GYLLAFISFRIFDILKPFPIRYFDEKLESGLGIMFDDILAAIFALISLHLLYYFI"
     gene            3649..4272
                     /locus_tag="PXD81_001495"
     CDS             3649..4272
                     /locus_tag="PXD81_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HDL5537512.1"
                     /translation="MLTILFIHFAGLISPGPDFFYVVRQSASHSVKSGVLAATGISIG
                     IIFWAAFAIFGLAWLSNSIGSLFQYAIMIIGGCYLVYIGSQMVRVTENIIFVENKAQL
                     TPLNPWQEIKKGLLINIFNAKAGVYFTSVISAYVDNLSNTLQMFELLLLFVVSTFIYF
                     CVVALLFSRRPVRLFYAKYSRYIDNISGVIFILFGSMLIYEGFSHFI"
     gene            4384..4989
                     /gene="ruvA"
                     /locus_tag="PXD81_001496"
     CDS             4384..4989
                     /gene="ruvA"
                     /locus_tag="PXD81_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005686751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HDL5537513.1"
                     /translation="MIGRLHGKIIEKQPPEILLDVQGVGYELLLPMTSFYSLPQIGEE
                     ATIFTHLVVREDAHLLFGFAQKQDRTLFRELIKTNGVGPKLALAILSAMSVSQFATAI
                     ENEELAKLTKIPGIGRKTAERLLVELKGKFKGMAQSDFFVEVSHEQIVASNTPDPANE
                     ALDALIALGYKPADAEKMIKKVNKSGATSEQLIREALKNSL"
     gene            5126..6130
                     /gene="ruvB"
                     /locus_tag="PXD81_001497"
     CDS             5126..6130
                     /gene="ruvB"
                     /locus_tag="PXD81_001497"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HDL5537514.1"
                     /translation="MIEADRIISASPKREEEVIDRAIRPKLLADYVGQPSVHDQMEIF
                     IKAAKLREEALDHLLIFGPPGLGKTTLANIVANEMGVNIRTTSGPVLEKAGDLAAMLT
                     NLEPYDVLFIDEIHRLSPAIEEVLYPAMEDYQLDIMIGEGPAARSIKLDLPPFTLVGA
                     TTRAGSLTSPLRDRFGIVQRLEFYSVDDLTLIVKRSADCLNLNLSADGAYEVARRSRG
                     TPRIANRLLRRVRDYADVRNNGIITSDIAKQALAMLDVDSEGFDFMDIKLLQAIVERF
                     DGGPVGLDNLAAAIGEERDTIEDVLEPYLIQQGFLQRTPRGRIATSRTYAHLGITQID
                     "
     gene            complement(6229..6912)
                     /gene="artM"
                     /locus_tag="PXD81_001498"
     CDS             complement(6229..6912)
                     /gene="artM"
                     /locus_tag="PXD81_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HDL5537515.1"
                     /translation="MWWDYLVEISQGIGTSLQLTFVALIIAFILAIILTFILSLENRP
                     LKWLVNGFITLFTGTPLLIQFFLIYSGPGQFEWITESAAWVLLSDAWFCAMLALALNS
                     AAYSTLLFHGAVKAIPKGQWETCAALGLSRLDTLKILVPYALKRALPSYSNEIVLVFK
                     GTSLASTITIMDIMGYARQLYGTEYDALSIYGMAGVIYLVITGIMTIVLRKLESRVLA
                     FERFETEKA"
     gene            complement(6912..7583)
                     /gene="artQ"
                     /locus_tag="PXD81_001499"
     CDS             complement(6912..7583)
                     /gene="artQ"
                     /locus_tag="PXD81_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HDL5537516.1"
                     /translation="MSSDILNLLVNATLMTLGIALASLLIGMLLSIFFVVLETSKIAC
                     IRKPTSIIITLLRGLPEILVVFLIYFGLIQVIFLITDEFAELNAFWCGAIALGIIFAA
                     YASQSLRGAIKAIPVGQWESGTALGLSRAQVFVRIILPQVWRHALPGLSNQWLVLLKD
                     TALVSLIGVDDLMRQSELINANTHQPFTWYGIAALIYLAITLISQRVIKVLEGRFTRF
                     ERGVA"
     gene            complement(7583..8308)
                     /locus_tag="PXD81_001500"
     CDS             complement(7583..8308)
                     /locus_tag="PXD81_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HDL5537517.1"
                     /translation="MKKLLLASAIAFATLSANAQETITFAMEPSYAPFETTNEKGEVI
                     GFDVDIAKALCEELKATCEFKTQSFDSLIAGLKFKNFDAIISGMDITEARQKQVAFTN
                     AYLENSVSYVAMKGKFTPETAKNVGVQNGTTFQQYLSKNAKQYNLKPYASLQSAVLDL
                     KNGRVDMVFGDTPVLAEWLKTEKELDFVGEKVVDKAFFGNGYGIAVNKSNTALLEKLN
                     SALATIKTNGKYEQIYNTWMAGK"
     gene            complement(8334..9068)
                     /gene="artP"
                     /locus_tag="PXD81_001501"
     CDS             complement(8334..9068)
                     /gene="artP"
                     /locus_tag="PXD81_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007523332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HDL5537518.1"
                     /translation="MAIRIQNVNFFYGSNQALFDINLEIEKGDTVVLLGPSGAGKSTL
                     IRTLNLMEVPQSGTLAIANHQFDLTAKTDSKAVSLLRREVGMVFQQYHLWPHLTVMQN
                     LIEAPMKVLGLSKEEAVARAKGHLARLRLSEFAERFPLHLSGGQQQRVAIARALMMQP
                     QVLLFDEPTAALDPEITAQVVDIIKELQSTGITQVIVTHEVGVARKVASKVVYMEKGT
                     IIEQGDASCFESPKTEQFAQYLSHSE"
BASE COUNT         2763 a         1911 c         1741 g         2657 t
ORIGIN      
        1 aaagcaatgt ggaatttgca aaaaatactg aaaacatcac cgcttgtaaa gtacctgata
       61 acctttctgc aaaaaatata gtaactgatt ttgacaaaat gacactgata ggattagtta
      121 aaatcaatga agtaatgcgt gcattattta ttgatgaaaa acaacaatta cttgctctaa
      181 aagaaaatga cttgcttaac aatgaaattg aaataaaaca tattaatctt aaatccatta
      241 cttatattaa ctggaaatta actaaagatt gtaatatccc ttatgaaatt aacatcaaat
      301 tatagaggtt gatatgtggc gaatttttat atatttctta tgcggttttt cattagccta
      361 tgcgaatacc atttccatag ttttaaaaaa tgctccaact tcgcttattt ttacttattt
      421 agcggaagaa accggtaaaa atatggtgct tgatgataat attgaaacca aatcaacact
      481 acgattagaa aataaatcgg ttgacgagat ttttaaaacg cttggcaaag tcaataaatt
      541 atccttaacg caagaagatg atattgttta tattcacaaa aaagaggaaa aagcggctga
      601 cttaacgcct ctgccaataa tgaatttgca aaataatggg caaaatacac cgcttgtcac
      661 cgcccctaag ttaatcacaa aaaccttgaa actacattat gcaaaggctt cagaggtgat
      721 tgagtcgtta accaaaggca gcggaacgtt tttatcggaa aacggttata ttcactttga
      781 tgaaagaagc aatagcctga ttgtgaaaga cagtgcaaaa tcgcttaaaa atatcgagaa
      841 tttagtaaaa cagctcgacc aacccaccga gcaaattgcg attgaggcta gaattgtcac
      901 tatcagtagc gagcatttgc aagagcttgg cgttcgctgg ggaatttttt ctcgtggggc
      961 tgggcactat aaatttggcg gacggctgga aggcaatgga ctcaacaatg tcaccaataa
     1021 cttaaatgta aattttccga ttactggtgg agcatctgcc gtattacagg tttcgtccat
     1081 taatagcaga gtgcttgatt tagaattaag tgcattagaa caagaaaata gtgtggaaat
     1141 tattgccagc ccacgcctgt taaccaccaa taaaaagccg gcaagtatca agcaaggcac
     1201 ggaaattcct tatgtgatgt acaacaccaa atcggaagcc accgatgtcg aatttaaaga
     1261 agcggtatta gggcttgaag tcgttccaca cctttcaacc caaaatcaaa ttctactcga
     1321 tctgatcgtt acccaaaatt cgccaaattc ccaatccggc agtagtggct taattacgat
     1381 tgataaacaa gagctaaata cgcaagtatt tgccaaacac ggcgaaacca ttgtacttgg
     1441 tggtattttt caacatttaa cccaaaaagg cgaagataaa gtgccgattt taggctcaat
     1501 tcccttcatc aagcggttat ttagtcaaac tcgagataaa atcagcaaaa gagagctagt
     1561 catttttgtg acgccttata tccttcaatc taatgaaaag agcagcaacc gcaaaaaatc
     1621 aaaatagcac atcataaacg caagcaaaaa taggggaagt gtgatagaat cagccgtcat
     1681 tttttgagtt aaagatttag aggttttatg aaaatttccc cacgtcgccg tgcaagagaa
     1741 tgtgcggtgc aagccattta ttcttggtat atttcccaaa acacggttga agaagtggaa
     1801 ttggcgtttg tgaccgatca agatatgaaa ggcgttgata tgccttattt ccgtaaactt
     1861 ttccgtggcg ttgtggataa tgtagaagcc attgatgaag cattacgccc ttatttagat
     1921 cgtaaagagt cagatgttga tcctattgaa cgttctattt tgcgtttatc aacttacgaa
     1981 ttaaaatttg aacacgatgt gcctttccgt gtagtcatca atgaagggat tgaagtcgct
     2041 aaagtgttcg gctctgatga cagccacaaa tacattaacg gcattttaga taaattagcc
     2101 ccagcattag gcagaaaata ataagattcc ccaatttata cataaattgg ggattttgat
     2161 tataacgctc aaggagattt tttatgggtg aatttgatat tatcgaacgc tattttaatg
     2221 cttctcaacg cccacctcgc aaagatgttt tactttctat cggcgatgac tgtgcgctca
     2281 catcactcaa accaaatcag caacttgcga tcacaaccga tacccttgtt tgcggcactc
     2341 attttctacc gactatttca cctgccgatc tcgcttataa aagtgtggcg tccaatttaa
     2401 gcgatcttgc cgcaatgggt gcagaacctg catggatctc tttagcctta accttacctg
     2461 aaattaatca cgattggcta gctgaattta gtgaacattt ctttgagatt cttgatcact
     2521 ataatgttga tttgattgga ggggatacta cttgtggacc actatctttg acaatcacag
     2581 ctcaagggat tgtgccacaa gatagaggct tattccgcca taatgctaaa gtaggcgatt
     2641 ggatctatgt ttccggcaca ctcggtgaca gtgcaggtgg tttgcaactt ctgctaaatc
     2701 agcaaagcga gcaagtgaat tggaataaag agcaaagcta cctgattcaa cgccatttac
     2761 gcccaacacc tcgagtttta cttggcttga ctatcgcctc tattgctaat gcagcgattg
     2821 atatttcaga tggtctattg tctgatttaa gccatatttt aaagcgcagt gactgtggtg
     2881 cagtattaaa ccttgagaat ttccccttgt ctgacgtctt acttagttgt tattctcgaa
     2941 cagaggcaga atattttgcc ttaaacggtg gcgaagatta cgaactctgc tttaccgtgc
     3001 caaatgacaa caaagccaaa ttagacaaag cattagccaa tatcggagta acttatacat
     3061 gtattgggca aattcgttcg gctaaagaag gtttaaccct attacgtcat aatcagcctg
     3121 tcaccttgcc ctcacaacga ggctacgatc attttaaaaa ataacgcaat gaaaccaatt
     3181 aatcttaaaa atccgattca tttttttgcc tttggctttg gttcaggctt attaaagcct
     3241 gctcccggca cctggggtag cctcgccggg ctaattgtag caatagcctt gtggtctatc
     3301 acacaatccg ccctgttttt cggcttactc accctagttt cttttattgc cggttgtgtt
     3361 atttgcacca aagccagcca agatctagaa gtacacgatg acgggcgaat tgtttgggac
     3421 gaaatagttg ccatcttttt gatgttcacc tgtttgcctg aatacaatct tcttggctac
     3481 ctgctcgcct ttatcagctt tcgcattttt gatattttga agccttttcc aatccgctat
     3541 tttgatgaaa aattagaaag cggtttaggc attatgtttg atgatattct cgcagccatt
     3601 tttgccctga tttcacttca tcttttatat tatttcattt aaaaatttat gctcactatt
     3661 ttatttattc attttgccgg gcttatcagc cccggccccg atttcttcta tgtcgtcaga
     3721 caatccgcca gccactcagt taaaagtggg gtacttgccg caaccggaat ctcaatcggc
     3781 attatttttt gggcagcctt tgcgattttt ggtttagcgt ggctcagtaa tagtattggc
     3841 tcgctgtttc aatatgccat tatgattatc ggtggctgtt acctggttta tattggctct
     3901 caaatggtac gggtaaccga aaatatcatt tttgttgaaa ataaagctca actcacgccg
     3961 ctaaaccctt ggcaggaaat taaaaaaggc ttattaatca atatctttaa tgccaaagct
     4021 ggcgtttact ttaccagcgt catttcagcc tatgtagata atttaagcaa taccctacaa
     4081 atgttcgaac tacttctact ctttgtggta agtaccttta tttatttctg tgtggtggca
     4141 ctactctttt ctcgccgacc ggtcagactg ttctatgcca aatatagtcg ctatattgac
     4201 aatatctcag gcgtaatttt catcttattc ggctcgatgc tgatttatga aggattcagc
     4261 cactttatct aatcacaagc ggtctgattt cactaaaaat ttgcaaattc cgctatcaat
     4321 aaatagtgtt attttataca gtttctgcta tgctactgcc aattttaact ctgagagaac
     4381 aaaatgattg gacgattaca cggcaaaatt attgaaaagc aaccgcctga aattttatta
     4441 gacgtgcaag gggtaggcta tgaactactg ctcccgatga ccagtttcta cagcctgcca
     4501 caaattggcg aagaggcgac tattttcacc catttagtgg tgcgagaaga tgctcacttg
     4561 ctattcggct ttgcccaaaa acaagataga actctattcc gtgaattaat taaaaccaac
     4621 ggtgtcgggc cgaagctcgc gttggcaatt ctatcagcaa tgtcggtgtc gcaatttgcc
     4681 accgcgattg aaaatgaaga gctggcaaaa ttaaccaaaa ttccgggaat cggtcggaaa
     4741 accgcagaac gcttattggt ggaactcaaa ggcaaattta aaggaatggc acagagtgat
     4801 ttctttgtgg aagtatcgca cgagcaaatt gtggcaagta atacgccaga cccggcaaat
     4861 gaagcccttg atgccttaat agccctaggc tacaaacctg ccgatgcgga aaaaatgatt
     4921 aagaaagtga ataaatccgg tgcaacgagc gaacagctta ttcgtgaggc gttaaaaaat
     4981 tcattatgat attgatagta tagggcgaaa gatatttcgc ccctacaatc tcatttttaa
     5041 aaatatatgt gtaggggcaa atcaatattt gccctcatga gtaatatagg gtaataccat
     5101 tccactacaa caataatcta aatgtatgat tgaagcagat agaatcatca gtgcctcgcc
     5161 taagcgtgag gaagaagtta tagaccgagc aatccgcccg aaactgcttg ccgactatgt
     5221 cgggcagcct tctgtgcatg atcaaatgga gatcttcatc aaagccgcta aattgcggga
     5281 agaggcatta gatcacttgc tcattttcgg cccgcccggt ttggggaaaa ctacccttgc
     5341 caacattgtg gcaaacgaaa tgggggtgaa tattcgcaca acttcagggc cggtgctgga
     5401 aaaagcaggc gatttggcag caatgctgac caaccttgaa ccttacgatg tgctgtttat
     5461 tgatgagatt caccgccttt ctccagcgat tgaagaagtg ctttatcctg caatggaaga
     5521 ttaccagctc gatattatga ttggggaagg acctgctgct cgttccatca agctcgatct
     5581 accgcctttt acccttgtag gggcaaccac ccgtgccggc tcactgacct caccgttacg
     5641 agatcgtttt gggattgtac aacgcttaga attttactca gtggacgatc ttactctgat
     5701 tgtcaaacgc agcgccgatt gcttaaatct caatttgtcg gcagacggtg cttatgaagt
     5761 ggctcgccgc tcccgtggca caccccgtat tgccaaccgc ttactccgcc gagttcggga
     5821 ttacgccgat gtgcgtaata atggcattat tacctcagac attgccaaac aagctctggc
     5881 aatgctcgat gtggattcgg aaggctttga ttttatggac attaaactgt tgcaagcgat
     5941 tgtagaacgc tttgatggcg ggccagttgg attagacaat cttgcggcag ctatcggcga
     6001 agaacgagat acgattgaag atgtgctaga gccttatctg attcagcaag gctttctaca
     6061 acgcacacca agaggcagaa ttgctaccag cagaacctat gcccatttag gtataaccca
     6121 aatagattag tcatatttct aaaggcattg atacataaat aaaaagcgga tagttagtac
     6181 tatccgcttt catttgcaaa aaatcaataa aatttgaccg cttatccgct aggctttttc
     6241 agtctcaaaa cgctcaaaag ctaatactct gctttctaat tttctgagaa cgattgtcat
     6301 aatgccggta attaccaagt aaataacccc tgccatacca taaattgata atgcgtcata
     6361 ctctgtacca taaagctggc gagcatagcc cataatatcc ataatcgtaa tggttgaagc
     6421 aagcgatgtg cctttaaaaa ctagcacaat ctcattacta taagaaggta aagcacgttt
     6481 taatgcgtaa ggcactaaaa ttttcagtgt atctaaacgg ctcaaaccaa gggctgcgca
     6541 agtttcccat tgccccttcg gaatcgcttt taccgcaccg tggaacaaca atgtcgaata
     6601 ggcagcactg tttaacgcta aagccaacat tgcacagaac caagcgtcgg ataataatac
     6661 ccaagccgca ctttctgtaa tccattcaaa ttgccccggg ccactataaa tcaagaaaaa
     6721 ttgaattaat aaaggcgttc cggtaaaaag cgtaataaat ccgtttacca accactttaa
     6781 cggacgattt tctaacgaga gaataaacgt aagaatgatc gctaaaataa aggcaatgat
     6841 gagcgccaca aaagtgagct gtaggctggt gccaataccc tgtgaaattt caactaaata
     6901 atcccaccac attaagcaac tcctcgctca aaacgggtaa atctaccttc taatacttta
     6961 atcactcgct ggctaattag agtaatcgct aaataaataa gtgctgctat cccataccaa
     7021 gtaaaaggtt gatgagtatt agcattaatt aactccgatt gacgcattaa atcatctacc
     7081 ccaattaacg ataccaatgc cgtatctttt aacagcacta accactgatt gcttaagcct
     7141 ggtaaagcgt gtcgccagac ttgagggaga ataatgcgaa caaatacttg cgctcggctt
     7201 aaccctaacg ccgtaccaga ttcccactgc ccaactggaa tagctttaat tgcaccacgt
     7261 aaagattggg aagcataagc agcaaaaata atacccagcg caatagcccc acaccagaaa
     7321 gcatttaact ccgcaaactc atcggtgatc aagaaaataa cttggataag accaaaatag
     7381 atcaaaaata ccactaaaat ctccggcaac ccacgcaata atgtaattat tatactagtt
     7441 ggtttacgga tacatgcaat cttactggtt tcaagtacca caaaaaagat tgacaataac
     7501 attccaatca agaggcttgc cagcgcaatc cctaaggtca tcagggttgc gttgacaagt
     7561 aagtttaaaa tatcgctaga cattatttcc cagccatcca agtattataa atttgctcat
     7621 atttaccatt tgttttgatg gtagctaaag ccgaatttaa tttttcaagc aatgcagtgt
     7681 tagatttatt taccgcaata ccatatccat taccaaaaaa ggctttatcg actacttttt
     7741 cacccacgaa atctaactct ttttcggttt ttagccactc tgccaatact ggtgtatcac
     7801 caaataccat atcaacacgt ccatttttta aatctaacac tgctgattgt aggctagcgt
     7861 aaggttttaa attatattgt ttggcatttt tgcttaagta ttgttgaaaa gttgtaccat
     7921 tttgcacgcc gacattctta gctgtttccg gtgtaaattt ccccttcatt gcgacataac
     7981 ttactgagtt ttctaaataa gcattggtaa aggcaacctg tttttgacgc gcttcagtaa
     8041 tatccatgcc tgaaataatt gcatcaaaat ttttaaattt taatcccgca attaagctat
     8101 cgaatgattg cgttttaaat tcgcaagttg cttttagttc ttcacataat gcttttgcaa
     8161 tatctacatc aaagccaata acttcacctt tttcatttgt tgtttcaaaa ggggcgtaag
     8221 aaggctccat cgcaaaagtg atagtttctt gtgcattggc agaaagcgtt gcaaatgcga
     8281 tagcggaagc taataataat tttttcattg ttgtgtcctt ttgttgttat agattactct
     8341 gagtgagata aatattgagc aaattgctct gttttcggtg attcaaaaca acttgcatca
     8401 ccttgttcaa taattgtacc tttttccatg tacaccactt tgcttgccac cttacgagca
     8461 acgcctactt cgtgagtcac aatcacctga gtaatgcctg tagattgcaa ttctttaata
     8521 atatcaacta cttgtgcagt aatttccggg tcaagtgctg cggtaggttc atcaaataat
     8581 aagacctgtg gctgcatcat cagtgctctg gcaatcgcca cccgttgttg ctgaccacct
     8641 gaaagatgca gtgggaaacg ctcggcaaac tcgctcaaac gcaaacgtgc caaatgtcct
     8701 tttgctctgg caaccgcttc ttctttgctt aacccaagca ctttcatcgg ggcttcaatt
     8761 aagttttgca ttacggttaa atgcggccat aaatgatact gctggaacac cattcccacc
     8821 tcacggcgta acaaactcac cgctttgctg tcggttttag cagttaaatc aaattgatga
     8881 tttgcaatcg ccaaagtgcc tgattgcggt acttccatta agtttaaggt tcgaattagg
     8941 gtacttttac ctgccccact cgggccaagc aatacaacag tatccccttt ttcaatctct
     9001 aagttaatgt caaataaggc ttggtttgag ccataaaaaa aattgacatt ttgaatacga
     9061 attgccattc gt
//
LOCUS       DANLYI010000092         8966 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.092, whole genome shotgun sequence.
ACCESSION   DANLYI010000092 DANLYI010000000
VERSION     DANLYI010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 8966)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8966)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8966
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.092"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            <1..333
                     /locus_tag="PXD81_001502"
     CDS             <1..333
                     /locus_tag="PXD81_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5537519.1"
                     /translation="VAYWQSVLKPIFGHRFSNFKDLYNTLKQIMRFRNRLYHQEVVWN
                     KRIAKKPTHALENLEKTYKQFELALQKIAPERFAFRQLSQALIWQRQIFFDQQIFEAE
                     IVILPQHI"
     gene            complement(369..965)
                     /locus_tag="PXD81_001503"
     CDS             complement(369..965)
                     /locus_tag="PXD81_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5537520.1"
                     /translation="MKKLLMTLCCLPAFALANETGESCSKIEDSAKRLECYDSVFVKK
                     DTGKDEATDEKSNWEYEQKKDELRNATTYFAKIRSTNTIDFGFPYNSSSMNLMLRKDP
                     KYGNDVIFSVHGQFNGCMIESCKITVKFDDGKLESYRMIGADGGSNDTLFIENAKAMK
                     TFVDKLKKSKKLIVEASFYNYGKGQFTFDTQGLEWKHF"
     gene            1611..1796
                     /locus_tag="PXD81_001504"
     CDS             1611..1796
                     /locus_tag="PXD81_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5537521.1"
                     /translation="MNKMKQLAPFALLILLLGIVGRMDYDDHIQMQRYKCERNQGFWQ
                     MESNGNQYCGGKYGTKN"
     gene            1780..1977
                     /locus_tag="PXD81_001505"
     CDS             1780..1977
                     /locus_tag="PXD81_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5537522.1"
                     /translation="MARKIDAGVEIVEVDKRFIAKFFYDGKLQHTTYPQYNRQNAIML
                     INRKIERFNIGRDKPIKLYKE"
     gene            1980..2954
                     /gene="ssb"
                     /locus_tag="PXD81_001506"
     CDS             1980..2954
                     /gene="ssb"
                     /locus_tag="PXD81_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDL5537523.1"
                     /translation="MAGINKVIIVGRLGNDPELRTMPNGDSVAKISVATSTEWTDKTS
                     GEKKQATEWHSIIAFRNLADIIGKYLKKGSQVYVEGKLRTRKWQAQDGTDRWTTEIIA
                     DQLQMLGSSNSGNSNNNWATEPAGNPPPTNPYNHVMTDSESRDFDDDIRVRWITKEQA
                     KQFINNISTDWMKTICSFALLTGARRTEILSMTWDKIDFIRKVAIVSNDIAKSEKARS
                     LLLNDDAIELLQKQRGKNPKYVFVGSKGQALQDINRKIFNKATEKCLLVDFHFHDLRH
                     TWASWHVQAGTPLFTLKELGGWETLEMVKKYAHLNADHLLNHANSVKF"
     gene            complement(3041..3117)
                     /locus_tag="PXD81_001507"
     tRNA            complement(3041..3117)
                     /locus_tag="PXD81_001507"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3081..3083),aa:Arg,seq:tct)
     gene            complement(3142..3218)
                     /locus_tag="PXD81_001508"
     tRNA            complement(3142..3218)
                     /locus_tag="PXD81_001508"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3182..3184),aa:Pro,seq:tgg)
     gene            3388..4248
                     /gene="folD"
                     /locus_tag="PXD81_001509"
     CDS             3388..4248
                     /gene="folD"
                     /locus_tag="PXD81_001509"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HDL5537524.1"
                     /translation="MTAQIISGSEVSSQIKKKVSEKIAHYLEQGKRNPGLAVILVGND
                     PASQVYVGSKRKSCAEIGIASKSYDLPEETTEAELLALIEKLNQDDSVDGILVQLPLP
                     KQIDSTKVIEAILPHKDVDGFHPYNVGRLSQRIPTLRPCTPYGVMKLLETTGVDLYGQ
                     HAVIVGASNIVGRPMALELLLGGCTVTVTHRFTKDLEHHVRQADILVVAVGKPEFIPG
                     EWVKDGAIVIDVGINRGADGKLKGDVEYSVASQKAAFITPVPGGVGPMTVAMLMQNTL
                     QTYENHLQAV"
     gene            4362..5813
                     /gene="thiI"
                     /locus_tag="PXD81_001510"
     CDS             4362..5813
                     /gene="thiI"
                     /locus_tag="PXD81_001510"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HDL5537525.1"
                     /translation="MKFIIKLFPEIMIKSDSVRKRFIKILTSNIRNVLLKETENVSVV
                     RHWDFIEVRAKVDEETALVLDLLKRTPGIHHILEVEETPFTDMHDIFEQTYAAVKAEL
                     EGKTFSVRVRRKGKHDFRSLDVERYVGGGLNQRIETAKVKLTNPDVTVRIDIEHDKML
                     LIKARHEGLGGYPIGTQEDVLSLISGGFDSGVSSYMLIRRGSRVHYCFFNLGGAAHEI
                     GVKQMAYHIWSRYSTSHKVRFIAINFENVVGEILEKVDNGQMGVVLKRMMVRAASRIA
                     ERFDIQAIVTGEALGQVSSQTLTNLRLIDKASDTLVLRPLITHDKEQIIAMAKAIGTD
                     DIAKSMPEFCGVISKNPTVKAIETKILEEEGNFNFEVLEQAVQTASYLDIREIAQQTE
                     KEVVSVETTTELSKNDVVLDIRSPEEVDEKPFRLDGVEVKELPFYKLSSQFSTLDQSK
                     TYLLYCQRGVMSKLQALYLKENGYSNVKVFRVK"
     gene            complement(5858..6565)
                     /locus_tag="PXD81_001511"
     CDS             complement(5858..6565)
                     /locus_tag="PXD81_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwiC-like family protein"
                     /protein_id="HDL5537526.1"
                     /translation="MFKEKPVISNQHGALAMAMLPFLYAVIESGFSTLHIFFGLSWLF
                     LYFFSYPFLSLFSKKPTERNKKWAKIYFGLSILFALPLIYAQPSILQFLPIILPLGLV
                     QVHYAKQKDERNLWNDIAGILTFGIMGMAAYYLVTKQYQFEILLHPTLFFIATTFYIK
                     SMVRERRNPIYMEISIAIHLVLAMLYILCSLQAVFFAYLIATARAIIVPSFGWNVKQV
                     GMLEFPVILLFLASLIW"
     gene            complement(6633..7607)
                     /gene="pfkA"
                     /locus_tag="PXD81_001512"
     CDS             complement(6633..7607)
                     /gene="pfkA"
                     /locus_tag="PXD81_001512"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007523004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HDL5537527.1"
                     /translation="MSKKIKKIAVLTSGGDAPGMNAAIRGVVRTALNEGLEVCGIQDG
                     YYGLYHDKVIPLDRRSVSETINRGGTFLGSARFPDFKRPEVRKHCVETLKKYSIDALV
                     VIGGDGSYMGAKLLTEEFGYPCIGIPGTIDNDIVGTDYTIGYQTALETALDAIDRLRD
                     TSSSHQRISIVEIMGRHCGDLTLSAALAGGCEYVIVPEKGLDKDALMRSIEEGFHKGK
                     RHSIIAITELMTDVHALAKEIEARFGNETRATVLGHIQRGGSPCAFDRILASRMGVYA
                     VELLLQGFGGHCVGIQNEQLVHHDIIDAINNMRRPFKEELFEASRKLF"
     gene            complement(7714..8910)
                     /locus_tag="PXD81_001513"
     CDS             complement(7714..8910)
                     /locus_tag="PXD81_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HDL5537528.1"
                     /translation="MLNTQFRTHFPFFEKSDGWTYLDSAATTLKPDVLIEATSEFYAS
                     AGSVHRSQYDLPQTEQYELARDLVAKRFNVETREAVIWTSGTTQSINTVAYGLEHQIA
                     AGDEIVISVAEHHANFIPWQQLAQRTRAKLVVLPLNDKYEIELETLKNALSEKTKIVA
                     FNLVSNVTGVRQPAEQIIPLIRQSCSAKIVLDIAQAVCSEQVDVQKLDADFYAFSAHK
                     MYGPNGVGVLTGKLQSLEQIQPLVFGGKMLKNITESELTVAELPYRLEAGTPNIAGII
                     GFGQVLAWLEKWDFVALNHQLYDLAEYTCKRLNSYQNLQIIGQQNTPTISFVVKNQHP
                     ADIAAFLTQSKIAIRQGEHCAKPYLRHLEEVGTVRLSLAHYNNQQDVEHFFNALDFTL
                     EILQES"
BASE COUNT         2795 a         1756 c         1864 g         2551 t
ORIGIN      
        1 gttgcttact ggcaaagcgt attaaagcct atttttggac atagatttag taattttaag
       61 gatttataca atacattaaa gcaaattatg cgttttagaa atcggctgta tcatcaagaa
      121 gtggtttgga ataagcgaat tgccaagaaa ccaacgcacg cactagagaa tttagaaaaa
      181 acttacaagc aatttgagtt agcactacaa aagatagcac ctgaacgatt tgcgtttcgt
      241 cagctttcac aagcacttat ttggcaacgc caaatctttt tcgaccaaca aatttttgaa
      301 gctgaaatcg ttattttgcc ccaacatatc taagcaaaaa gctacgcatc tatgtagctt
      361 tttcttaatt aaaaatgttt ccactctaac ccttgtgtat caaaggtaaa ttgccctttg
      421 ccataattgt aaaaactcgc ctctacaatt agttttttcg actttttcaa cttatctaca
      481 aaggttttca ttgcttttgc attttcaatg aaaagcgtat cattgctgcc accatccgcc
      541 ccaatcattc gataactttc caatttacca tcatcaaact tcacagtaat tttacagctc
      601 tcaatcatac agccattaaa ttgaccgtgt acgctaaaaa ttacatcatt gccatattta
      661 gggtctttgc gtaacattaa attcattgat gatgaattat acgggaaacc aaaatctatt
      721 gtgttagttg atctaatttt agcaaagtaa gtcgttgcat tacgcaattc atctttcttt
      781 tgttcatatt cccaattaga cttttcatcg gtagcttcat ctttaccagt atcttttttc
      841 acaaagacac tatcataaca ttctaagcgt tttgcactat cttcaatctt agagcaactt
      901 tctccagttt cgttagctaa agcaaaagcg ggcaaacaac ataaagtcat cagtagtttt
      961 ttcatataac ctccatagaa atcccttctt attttctcaa aagcgtggtt ttcggtctgt
     1021 gatcaagctc gcaatctcaa taattatatt ttaatggttt aaaaaacaat caattaagaa
     1081 atcataacta attgaaattt aagaaatctt aaaaatactt attaaataag cttaaatttt
     1141 gattgacttg taatttaagt tttcttaaac ttaattcatc aaaacgagct acggctcaat
     1201 gctctttaac aattcggtgc ttgtgcggac gatattaaca acctcgggca gttgtaaagt
     1261 aatgctttat aactcagtaa ggttggttag accctgacat acaaatttga acgacttgcc
     1321 aaaaagaggt ggcaaggttt aggcaacaca ctcgcaaggt gtggagagct gaaaaggcaa
     1381 cactggtatc agtcctaagc aatcacgctt atctcaagtt tgagtaagta cggatacaag
     1441 cagcacccga cggaatggcg aaagccgggc gggcgacggc gtgggcagac acgcatttat
     1501 tccaaagctc atttcaccaa gtgtgtttcg gaatacccac aactagacgc aagtcgctcc
     1561 atttgcccac cgtaaaacgt gggctttttt taaccctaaa ggacaatcct atgaacaaaa
     1621 tgaaacaact tgccccattt gccttgctaa ttttgctatt aggcattgtc gggcgaatgg
     1681 attatgacga ccacatacaa atgcaacgtt acaaatgcga acgaaatcaa ggcttttggc
     1741 aaatggaaag caacggcaac caatactgcg gagggaaata tggcacgaaa aattgatgcc
     1801 ggtgttgaga ttgttgaggt agataaacga tttatcgcca agttttttta tgacggcaaa
     1861 ttacagcaca caacctatcc acaatacaac cgccaaaacg caattatgtt gattaaccgc
     1921 aaaattgagc ggttcaacat tggcagagac aaaccaatca aactttataa ggaataacaa
     1981 tggcaggaat taacaaagtg attattgtcg gtcgcctagg taacgacccg gaactacgaa
     2041 caatgccaaa tggcgattca gtcgccaaaa tcagtgtagc aacttcaacc gaatggacgg
     2101 ataaaacctc aggcgagaaa aaacaagcca cagagtggca tagcattatc gcctttcgga
     2161 atttagctga cattatcggc aaatacctga aaaaaggctc gcaagtgtat gtggaaggca
     2221 agctacgcac tcgcaaatgg caagcccaag acggcacaga ccgctggacg accgaaatca
     2281 tcgccgatca gctacaaatg ctcggtagct caaacagcgg aaacagcaat aataattggg
     2341 caaccgaacc ggcaggaaac ccaccaccaa ccaatccata taaccacgta atgacggatt
     2401 cagaatcacg agattttgat gatgatatcc gggttcgttg gattaccaaa gaacaagcca
     2461 agcagtttat taataatatt agtactgatt ggatgaaaac tatctgctct tttgctttat
     2521 taacaggagc aaggcgaaca gaaatacttt ctatgacttg ggataaaata gattttatcc
     2581 gtaaagtagc gattgtttcg aatgatattg ccaaatctga aaaagcgaga tcgcttttac
     2641 ttaatgatga tgcaattgaa ttattacaaa agcagagagg gaaaaatcct aaatatgttt
     2701 ttgtaggaag caaagggcag gcattacaag atattaaccg caaaatattt aacaaagcga
     2761 ctgaaaaatg tttattggtt gatttccatt tccatgattt aaggcatact tgggccagtt
     2821 ggcacgttca ggccggcaca ccattattta ccttaaaaga attgggtggc tgggaaacat
     2881 tagagatggt aaagaaatat gcccatctta atgcagacca tttactcaac cacgctaact
     2941 cagttaaatt ctagtcacaa attagtcaca aagtattttt gacataggaa attaataact
     3001 gatatttaaa tgtaactatt tgaaatataa gggtaattaa tggcacgccc taaaggattc
     3061 gaacctttga cccacgcctt agaagggcgt tgctctatcc agctgagcta agggcgcaga
     3121 tgaagataat ataaaaagaa gtggtcggcg agataggatt tgaacctacg acccactggt
     3181 cccaaaccag ttgcgctacc aagctgcgct actcgccgac ataagatgtt gttgattata
     3241 gatttctttg cacaacagtc aatctttttt tgtgaatgtc ggcttaaatg attgaagttt
     3301 atccgttatt caaaattagc ttatcaaaaa ggctattttt tgagagaatg acgaatgttt
     3361 tgccaagtga tatttcggga ggtagtaatg actgcacaaa ttatttcggg gagtgaggtt
     3421 tcttcacaaa ttaaaaaaaa ggtgagtgag aaaatcgctc attatcttga acagggtaaa
     3481 cgtaaccctg gtcttgcggt cattttagtg gggaatgacc cagcctctca agtgtatgtg
     3541 gggagtaaac gcaaaagctg tgccgaaatt gggattgcgt cgaaatctta tgatttaccc
     3601 gaagaaacca cagaggctga attattagca ctgattgaaa agttgaatca agatgattcg
     3661 gtcgatggga ttttagtcca attaccgtta cctaaacaga tcgattcgac taaagtgatt
     3721 gaagccatct taccacataa agatgttgat ggattccacc cttataatgt tgggcgttta
     3781 tctcagcgta ttcccacttt acgcccttgc acgccatacg gtgtgatgaa gttgcttgaa
     3841 acaactgggg tagatttata tggtcaacac gcagtgattg taggtgcgtc taatattgtt
     3901 ggtcgcccga tggcgttaga attgttgctt ggtggttgta cagtcacagt tactcatcgc
     3961 tttaccaaag atttagagca tcacgttcgc caagcagata ttttagttgt ggcagtcggc
     4021 aagcctgaat ttattcccgg ggagtgggtt aaagacggtg ctatcgtgat tgatgtaggg
     4081 attaaccgtg gagcagacgg caaactaaaa ggtgatgtag agtattcagt tgcctcacaa
     4141 aaagcggcat ttatcacccc tgtccccggt ggggtaggcc cgatgacggt ggcaatgtta
     4201 atgcaaaata ccttgcagac gtatgaaaac catttacaag cggtctaatt ttacggatat
     4261 tttgcaaatc ctcggcattg gtcgaggatt tctttttctg ggctttttca gcagaaaagg
     4321 ctaaaatact tgccattttt attacacgat ccttattaaa tatgaaattt atcatcaaac
     4381 ttttccctga aattatgatt aaaagcgact ctgttcggaa acgctttatc aaaattttaa
     4441 cctcaaatat tcgcaacgta ttattaaaag agactgaaaa tgtgtctgtg gttcgccatt
     4501 gggactttat tgaagttcgg gcgaaagtgg acgaagaaac ggcattagtg ctggatttgc
     4561 ttaaacgcac gccgggtatt caccatattt tagaagtgga ggaaacccct tttacggata
     4621 tgcacgatat ttttgagcag acttatgccg cagtgaaagc tgaactagaa ggcaaaacgt
     4681 ttagcgtgcg ggttcgccgt aaaggaaagc acgatttccg ttctcttgat gtagagcgtt
     4741 atgtcggtgg tgggctgaat cagcggattg aaacggcgaa agtgaaatta accaacccag
     4801 atgttacggt cagaattgat attgaacatg ataaaatgtt gctgattaaa gctcgccacg
     4861 aagggttagg cgggtatcca atcggcacac aagaagatgt gttgtcattg atttcgggtg
     4921 gttttgattc gggtgtatcg agctatatgc tgattcgcag aggttctcgt gtgcattact
     4981 gttttttcaa tttaggtggt gccgcacacg aaattggcgt gaaacagatg gcatatcata
     5041 tttggagccg ttatagtacc tcgcacaaag tccgttttat cgccattaat tttgaaaatg
     5101 tagtgggtga gattttagaa aaagttgata acggacaaat gggcgtggta ttaaaacgta
     5161 tgatggtgcg agcggcaagc cgtattgccg agcgttttga tattcaagcg attgtgaccg
     5221 gtgaggcgtt ggggcaggtc tctagccaaa cactgactaa tttacgttta attgataagg
     5281 catcagatac gctggtatta cgtccgctca tcactcacga taaagagcag attattgcaa
     5341 tggcaaaagc gataggcact gacgatattg ctaaatcaat gccggagttc tgtggagtaa
     5401 tttctaaaaa tccgacggtt aaggcaatcg aaactaaaat tttggaagaa gaaggcaact
     5461 ttaattttga ggtactagaa caagcggttc aaacggcgag ttatttagat attcgtgaga
     5521 tcgctcagca aaccgaaaaa gaggttgttt cggttgaaac cacaacagaa ttgagtaaaa
     5581 acgatgttgt gctagatatt cgcagcccgg aagaggttga tgaaaaacca ttccgtttag
     5641 acggggttga agtaaaagaa ctcccgtttt ataaactttc cagccagttt agtacgcttg
     5701 accaaagcaa aacctacttg ctctattgcc aacgtggggt aatgagtaaa ctgcaggctc
     5761 tttatttgaa agagaatggc tacagcaatg taaaagtgtt tcgagtgaaa taatgcctct
     5821 ttgattgcac cttaatgatt gattaaggtg caatttttta ccaaataagg cttgctaaaa
     5881 agagtaagat cacaggaaat tcaagcattc caacttgttt aacattccag ccaaagcttg
     5941 gcacaataat ggctcgggca gttgcaatta aataagcaaa aaagaccgct tgtagagagc
     6001 agagtatgta aagcattgct aaaaccaaat gtattgcaat actgatttcc atataaattg
     6061 ggtttcttcg ctctcgcacc atactcttta tatagaaagt cgtagcaata aaaaataggg
     6121 tagggtggag cagaatctca aactgatatt gtttcgttac cagataataa gctgccattc
     6181 ccattatgcc aaaggttaga atgccggcaa tatcattcca taaattgcgt tcatcttttt
     6241 gtttggcgta gtgaacttgc actaacccaa gcggtagaat tatcggtaaa aattgcaaaa
     6301 tcgaaggctg agcgtagatc aaaggtaatg caaatagaat gcttaagcca aagtagattt
     6361 tcgcccattt tttattgcgt tcagttggct ttttactgaa aagtgagaga aaagggtagg
     6421 aaaagaagta gagaaacagc cacgacagcc caaagaaaat atgcaaagtg ctaaaaccac
     6481 tttcaataac ggcgtagaga aacggcaaca ttgccattgc taatgcaccg tgttggttgg
     6541 atataacggg tttttctttg aacattcaat ttccctctta atgtggcaaa taaaaaggcg
     6601 actaaacagc cgcctttaaa ttgtaagaaa agttagaata attttctaga ggcttcaaat
     6661 aattcttctt tgaacggacg acgcatatta ttgatcgcat caataatatc gtggtgaacc
     6721 aactgctcgt tttgaatacc gacacagtga ccgccaaagc cttgcagtaa taattccacc
     6781 gcatagacgc ccatacggga ggccaaaata cggtcgaagg cacaaggcga accaccacgt
     6841 tgaatatgac ctaatacggt tgcacgggtt tcattgccga aacgtgcttc aatttctttc
     6901 gctaatgcgt gaacatcggt catcaattct gtaattgcaa taattgaatg gcgtttacct
     6961 ttgtggaaac cttcttcaat gcttcgcatt aatgcatctt tatctaagcc tttttccggt
     7021 acgatcacat attcgcaacc accggcgagg gcagcactta gggttaaatc accacagtgg
     7081 cgacccataa tttccacaat cgaaatacgt tggtgtgaac ttgaagtatc acgcaggcgg
     7141 tcaatcgcat ctaatgcggt ttcaagggca gtttgatagc cgatggtgta atccgttccc
     7201 acaatatcgt tatcaatcgt acccggaata ccgatacaag ggtaaccaaa ttcttcggtt
     7261 aataatttag cccccatata agaaccgtcg ccaccaataa ccactaatgc atcaatgctg
     7321 tattttttta aggtttcgac acaatgtttg cgaacttctg gtcttttaaa atcagggaag
     7381 cgggcagaac ctaagaatgt accgcctcgg ttgatggttt cagacacgga acggcggtca
     7441 agtgggatca ctttatcgtg gtaaagccca tagtagccgt cttgaatacc acaaacctct
     7501 aaaccttcat taagtgcggt acgaaccaca ccacggattg ccgcgttcat tcctggtgca
     7561 tcaccaccac tggttaatac tgcaattttt ttgatttttt tactcattta aaaacacctt
     7621 gttgataaat taaagttaaa ttggaaactg gtgcaaggtt acaatattca gccttttcgt
     7681 tctactaaaa aatacgtatt tttttgattt agcttagctt tcttgcaaaa tttcaagggt
     7741 aaaatctaga gcgttgaaaa aatgctcaac atcttgttga ttattgtaat gggcaagtga
     7801 aagtcgaact gttccgacct cttctaaatg gcgtaaataa ggcttggcac aatgctcccc
     7861 ttggcggatc gcaattttac tttgggttaa gaaagcggca atatcggcag ggtgctgatt
     7921 tttcaccaca aagctaatag tcggtgtatt ttgttgcccg ataatttgca aattttgata
     7981 agaatttaac cgcttgcagg tgtattcagc caagtcataa agctgatgat taagagcgac
     8041 aaaatcccat ttttcgagcc aagctagcac ttgcccaaag ccgataatgc cggcgatatt
     8101 cggcgtgcca gcttccaagc gataaggtaa ctctgccaca gttaattcac tttccgtaat
     8161 gtttttcagc attttgccgc caaatacaag cggttgaatt tgctccaaac tttgcaattt
     8221 gccggtaagc acgcccacac cgtttggacc atacatttta tgggcggaaa aagcgtagaa
     8281 atctgcatct aatttctgaa cgtccacttg ctctgagcaa actgcttgag caatatctaa
     8341 cacaatttta gcagagcaag attggcggat aagcgggatt atttgttccg ctggctggcg
     8401 aactcctgtt acgttagaaa ctaaattaaa ggcaacaatt ttggtttttt ccgataacgc
     8461 atttttaagg gtttcaagct caatttcata cttatcatta agtggcaaca ccacgagttt
     8521 cgcccttgta cgctgtgcca actgctgcca aggaataaaa ttagcgtgat gttccgccac
     8581 tgaaattaca atttcatcgc ctgctgcaat ttgatgctcc aagccataag ccacggtgtt
     8641 aatactttgg gttgtaccgc ttgtccaaat caccgcttca cgagtttcca cattgaaacg
     8701 ttttgcgact aaatctcgag ctaattcata ttgttcggtt tgtggcaaat cgtactgact
     8761 gcgatgcacc gaccccgcag aagcgtaaaa ctcacttgtt gcttcaatga gtacatcagg
     8821 tttcagcgtt gtagcggcag aatctaaata cgtccatccg tcagattttt caaaaaaagg
     8881 gaaatgggtg cgaaattggg tgttaagcat aagttaattc ctaagagttg tggaaaattg
     8941 agttaccaag aaaataccta ttataa
//
LOCUS       DANLYI010000093         8899 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.093, whole genome shotgun sequence.
ACCESSION   DANLYI010000093 DANLYI010000000
VERSION     DANLYI010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 8899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8899
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.093"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            <1..166
                     /gene="zapB"
                     /locus_tag="PXD81_001514"
     CDS             <1..166
                     /gene="zapB"
                     /locus_tag="PXD81_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011201404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cell division protein ZapB"
                     /protein_id="HDL5537529.1"
                     /translation="TIQLLQLEVEELKGKNDAAKQENDTLRSEYEQLKVEQQNFQDRL
                     RSLLGQIDNV"
     gene            222..803
                     /locus_tag="PXD81_001515"
     CDS             222..803
                     /locus_tag="PXD81_001515"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014064645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HDL5537530.1"
                     /translation="MAKLYFYYSSMNAGKSTTLLQSSYNYQERGMNTLVYTAAIDDRF
                     GVGKVNSRIGISQEANLFTADTNLFAEIKALHSNKALHCILIDEAQFLTKTQVYQLSD
                     VVDKLKIPVLCYGLRTDFQAELFEGSKYLLAWADELEELKTICDCGKKAHFVVRLNEK
                     GEAIKDGEQIQIGGNDRYLSVCRYHYKQKLGKL"
     gene            complement(894..2012)
                     /locus_tag="PXD81_001516"
     CDS             complement(894..2012)
                     /locus_tag="PXD81_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HDL5537531.1"
                     /translation="MTTTYFHLMAKPSSFHCNIQCEYCFYLSKEQTIPPEKSQFMNDE
                     TLQNYIRHYIEANQSQRVDFVWQGGEPTLLGLDFYKQAVKYQQKFANGKQITNAIQTN
                     GIALNRQWASFFKENHFLVGLSIDGLSDIHDRYRISTNGKPTFERVKSAVDLLKEVGV
                     EFNTLTVVNDRNWQRGRETYQALKALGSRYMQFIPVVESHKGELQPFSVPPQGYGQFL
                     VDVFHEWLPDRGQIFVIEFDNLLGQWLGYPSNNCVFRPTCGSSLVVEACGDVYSCDHF
                     VYPDYHIGNLNQQQLAEIVSSAAQRQFGQNKQAKLSQACLNCKFRILCHGGCPKHRIV
                     PQANSENHNYLCPSYQQFFAETSSVMRQMSEQIRFAGK"
     gene            complement(2102..3550)
                     /locus_tag="PXD81_001517"
     CDS             complement(2102..3550)
                     /locus_tag="PXD81_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005822114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HDL5537532.1"
                     /translation="MNNIIYILLDQVRKDMLGTYGHQVVKTPNLDRLAKDGIRFNNAF
                     TPASVCGPARTSLFTGLMPSSHGIIKNGEKGGTGEIPQQNPNIAGVEGYNSFVIGKWH
                     VGTKSVPEDYGIAGHNFDGYGYPGSQVYKNLVFNQPPTHSNRYREWLAEKGYDAPEVS
                     RAYFGDNPHLRVQELCGLLSGSREQTIEWFIIDEAKKYIQASLDEKKPFFAWINFWGP
                     HTPCVVPEPYYSMYDPSQVVLDESFFKPLEGKPNHFRTISQMWGMWEASEDRWKEVIS
                     KFWGYITLIDDAIGELFRFLEENNLYENTFLVATADHGDAMGAHKMIEKGEFMFETTY
                     NIPLIIKDPHSTRVNEVDDNLVYLHDLTSTVYDVANQPIPTAFQGESILPITRQSQNN
                     DRKGLLCQLAGHFVYFEQRMWRRKDYKLVFNATDRCELYDVRKDPEEMHNLFYDEAYQ
                     AVKKEMLEEMRQEMKRLNDPLENWVYRIIDEI"
     gene            complement(3697..5394)
                     /locus_tag="PXD81_001518"
     CDS             complement(3697..5394)
                     /locus_tag="PXD81_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HDL5537533.1"
                     /translation="MLLTIITFLVVTGLVGYISWLKTRNDDLSTSKGYFLAGRGLSAT
                     VIGCSMVLTSLSTEQLIGVNANSYAGNFSIIAWTVPAVIPLCFLALYLLPKYIRNGYT
                     TIPEFFENRFDRQTRLIMSGLFLVFYLFIVIPTALYTGAIAFNKIFNLETAFGLSYGA
                     AITYTVIAIGIVGAIYAIFGGLKAVAVSDTWNAVLLVIGALLVPVFALIYLGNGSMAE
                     GVKIITTTHVEKWNAVGSATDAVPWPTIFTGIMVVHFFYWTTNQAIVQRALGARDLKA
                     GQKGILIAALFLLLLPLILNLPGLLSFHILGEGLKPIDVSYPELVNKTFPTVLQGFFI
                     AALFGAILSTFNSFLNSAATIYCKDLLPSISKKQFTDAELISYAKKVSAVMAVITMIF
                     GPLLMFGTDGIFLLTKRFAGFVNIPIVALFVIGLFNKTVSGKAARIALLAHVILYFCI
                     VWVFDVKINFVYVQAGLFVFDVALMLILGIFLKRATPYEDNKINHANVDLTSWEYAPL
                     AVASIVLGLFTLYAFLSPLGLASPDGNPAMVLAVYAVLQAVVCVVFFAKCKGKKAVN"
     gene            complement(5538..6089)
                     /locus_tag="PXD81_001519"
     CDS             complement(5538..6089)
                     /locus_tag="PXD81_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HDL5537534.1"
                     /translation="MNNMPTPEQLMSCQLSVMSDLEKGVVIYSQGYHAREFYFLKSGL
                     IGLYHMLENGKESLVRVYKANDYFGFRTFFGDNRYHCSARVLQPAELIRITPLNFIEF
                     WSNNSALIISLLKQLSTELQDAEHRLSMVSYQKTQERVMNSIHYLHQRYPNYHWTNRE
                     IAEFAGCETETAIRLRRELKKIS"
     gene            6188..6997
                     /locus_tag="PXD81_001520"
     CDS             6188..6997
                     /locus_tag="PXD81_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HDL5537535.1"
                     /translation="MTMTVIIILIICGIIVNITSALFGIGGGVLMMPILHSLFPELPL
                     QVISACSLTIVIGSATINLCLFHHQQIKINRKNVLIWSLAMMLGVQIGFELSFLFSDN
                     SIIAIFILTLLTLSFKTLFHKPRVKAIVPPKPKEAVSNATMCSLGGLIAGITGIGGGS
                     IMAPIVTQIRSVSPKQVAVYTNYMMVIGGIGNLYGYLSRPFVLNSTLATWQIGYVNFA
                     IVGTVVISSLFTVYFSMKLRNILSPELTTKLLGVILFTIACYMGFLQYLFT"
     gene            complement(7014..7706)
                     /gene="tsaB"
                     /locus_tag="PXD81_001521"
     CDS             complement(7014..7706)
                     /gene="tsaB"
                     /locus_tag="PXD81_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009875421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HDL5537536.1"
                     /translation="MKPSILALDTATEACSVALQYNGNITFLDEISPRSHTQRILPMV
                     DELLNQANIELKEVNYLVFGRGPGSFTGVRVGVSVAQGLAMGANLPVVAVSNLKAMAE
                     EAYQKLGATKVIALIDARMNEVYFAQFERKGEEWNEVVAEQVCSPEVAISQFQMSEET
                     VVVGTGWAAYSQFSEQNLPLVISDITLPSSRYMLSIAETEIQKGNIQSAFEIEPVYLR
                     NEVTWEKLPHKQ"
     gene            complement(7793..8875)
                     /gene="aroG"
                     /locus_tag="PXD81_001522"
     CDS             complement(7793..8875)
                     /gene="aroG"
                     /locus_tag="PXD81_001522"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HDL5537537.1"
                     /translation="MSYIKNDDVRITSIEELLPPIALLEAYPASDVAAETVAKTRQSI
                     HKILHGADDRLLVVIGPCSIHDPKAALEYAQKIKAMRADPKINQNLEVVMRVYFEKPR
                     TTVGWKGLINDPYLNETFALNDGLRIARKVLSDINDLSVPTAGEFLDMITPQYVADFM
                     SWGAIGARTTESQVHRELASGLSCAVGFKNGTNGSVKIALDAISSAKSPHHFLSVTKF
                     GHSAIVSTAGNPDCHIILRGGDSATNYDADSVAAACAAIEKSGGRPHVMIDFSHANSQ
                     KQFKRQMDVCADVCNQIAGGSDFISGVMIESHLVEGRQDLIEGKGLEALVYGQSITDG
                     CIGWEDSEKALFALAEAVEKRRNFAK"
BASE COUNT         2459 a         1980 c         1880 g         2580 t
ORIGIN      
        1 aactattcaa ttacttcaat tagaagttga agaattaaaa ggtaaaaacg atgcagcgaa
       61 acaagaaaac gacaccttgc gcagcgaata tgaacaatta aaagtggaac aacaaaactt
      121 ccaagatcgt ttacgttcac ttttaggtca aatcgacaac gtttaattct attttcagca
      181 caaacggcta aatttttagc cgttttttat ttgttacagc tatggcaaaa ctctattttt
      241 actactcctc aatgaatgca ggcaaatcta ctactctgct tcaatcttct tataattacc
      301 aagaacgtgg aatgaacact ttagtttata ccgccgctat tgatgaccgt ttcggtgtgg
      361 ggaaagtcaa ctctcgtatc ggtatttcac aagaagccaa tttatttacc gctgatacca
      421 atctatttgc ggaaattaag gcattacact caaacaaagc gttacactgt atcttgattg
      481 atgaggctca gtttttaact aaaacacagg tttatcaatt aagtgatgtg gtcgataaac
      541 tcaaaattcc ggtgctttgc tacgggctac gcaccgattt ccaagctgaa ttatttgaag
      601 gtagcaaata tttgttggct tgggcagatg agctggaaga actcaaaacc atctgtgatt
      661 gtggcaaaaa agcccatttt gtcgttcgat taaatgaaaa aggcgaagcc attaaagacg
      721 gtgaacaaat acaaatcggc ggcaatgata gatatctctc tgtttgccgt tatcattaca
      781 aacaaaagtt aggtaagcta taagctacaa gaagcagcaa gcggttaaat tttcgggaat
      841 ttttgcaaat tttcagcgaa atttaaccgc ttgtttgtgt tatttacctc tctttacttt
      901 cctgcaaacc gaatttgctc actcatttgt ctcatcacac tcgaagtctc ggcaaaaaac
      961 tgttggtaag aggggcaaag gtagttgtgg ttttcgctgt ttgcttgggg gacgatgcgg
     1021 tgtttggggc agccgccgtg gcaaagtatg cggaatttgc agtttaggca ggcttgcgag
     1081 agttttgcct gtttattctg cccgaattgg cgttgggcgg ctgatgagac gatctctgcc
     1141 agctgctgtt ggttgaggtt gccgatgtgg taatcgggat agacgaaatg gtcgcaactg
     1201 tacacatcgc cacaggcttc caccaccagc gagctgccac aggtcgggcg gaatacacag
     1261 ttgttggagg gatagccaag ccattgcccg agcaggttat caaactcgat cacaaaaatc
     1321 tgcccacggt caggcagcca ttcgtgaaaa acatccacta aaaattgccc gtagccttgg
     1381 ggcggaacgg aaaagggttg taattccccc ttgtggcttt ccaccaccgg aatgaactgc
     1441 atataacggc taccgagggc tttgagggct tgataggttt ctctgccccg ttgccagttg
     1501 cggtcgttca ccaccgtgag ggtgttaaat tccacgccaa cctctttcaa taaatcgacc
     1561 gcacttttca cccgttcaaa ggtcggttta ccgttggtgg aaatgcggta gcggtcgtga
     1621 atgtcgctca gtccgtcaat ggataagccc accagaaaat gattctcttt gaaaaagctc
     1681 gcccactggc ggtttagggc aatgccgttg gtttggattg cgttggtgat ctgcttgccg
     1741 tttgcaaatt tttgttgata tttgaccgct tgtttgtaga aatctaagcc cagcagggtc
     1801 ggctcgcccc cctgccacac aaaatccacc cgttggcttt ggttggcttc aatgtaatga
     1861 cgaatgtagt tttgcagcgt ttcatcgttc ataaactgcg atttttctgg cggaatggtc
     1921 tgttccttgc tcagataaaa acagtactcg cactgaatat tacagtgaaa actgctgggt
     1981 tttgccatta ggtggaagta ggtggtggtc attgtatctc taaattgtag gggcggactt
     2041 tatgttcgcc cattgtttac attaattttc gggcgggtat aaaacccgcc tctacaaaca
     2101 attatatctc atcaataatc cgatacaccc aattttccag tgggtcgttg aggcgtttca
     2161 tttcctgtcg catttcttcc aacatctctt ttttcacggc ttggtaggct tcgtcataaa
     2221 agaggttgtg catctcttcg gggtcttttc tgacatcgta gagttcgcag cggtcggtgg
     2281 cgttgaagac caatttgtaa tccttacgcc gccacattcg ctgttcaaaa tagacaaaat
     2341 gccctgccag ttggcacaac aagcctttgc ggtcgttgtt ttggctttgt ctggttatcg
     2401 gcagaatact ctcgccttgg aaagcggtcg ggatcggttg atttgccaca tcatacacgg
     2461 tggaagtgag atcgtgcaaa taaacgaggt tgtcatcgac ttcgttcacc cttgtagagt
     2521 gcggatcttt aataatcagc ggaatgttgt aggtggtttc aaacataaac tcgccttttt
     2581 cgatcatctt atgcgccccc atcgcatcgc cgtggtcggc ggtagcaacg aggaaggtat
     2641 tttcatagag attgttctct tccaagaaac ggaacagctc gccaatggca tcgtcaatca
     2701 gcgtgatgta gccccagaat ttagaaatca cctctttcca acgatcttcg ctcgcctccc
     2761 acattcccca catttgtgaa atggtgcgga agtggttcgg tttgccctca agcggtttga
     2821 agaaactctc gtccaatacc acttggcttg ggtcatacat tgaataatac ggctccggca
     2881 ctacacacgg agtgtgcggc ccccagaagt tgatccacgc aaagaacggt tttttctcat
     2941 caagggaggc ttgaatgtat tttttcgcct cgtcaatgat aaaccactct attgtctgct
     3001 cgcgactgcc ggaaagcaaa ccacagagtt cctgcacacg caggtgtgga ttgtcgccaa
     3061 aataggctcg actgacttcg ggtgcgtcat agcccttttc cgccagccat tcacgataac
     3121 ggttggagtg tgttggcggc tgattaaaca cgaggttttt atacacttgg ctgcccgggt
     3181 agccatagcc gtcaaagtta tgaccggcga tgccataatc ttcgggaacg gattttgtac
     3241 caacgtgcca tttgccgatc acaaaggagt tatagccctc cacgccggca atattagggt
     3301 tttgttgtgg aatttcgcct gtgccgcctt tttcgccgtt tttgataatc ccgtggctgg
     3361 acggcattaa gccggtaaac agcgaggtgc gagccggccc gcagaccgaa gccggcgtaa
     3421 aggcgttgtt aaaccgaatg ccgtctttcg ccaagcgatc caaattcggg gtttttacca
     3481 cctgatgccc gtaagtgccg agcatatctt tacgcacttg atctaacaaa atatagatga
     3541 tgttattcat aatgacctct ttaaggcttg ttaataaccg ataaattatc aagtttcgga
     3601 gatctgttcc cctctttagc aaagagggga tttttgatct cgagcctttt ccgttcttgg
     3661 tatttttata ctaatgagga gaaggctcac attcatttaa ttcaccgcct ttttgccttt
     3721 acattttgca aaaaacacga cacaaaccac cgcttgtagc acagcataca ccgccagcac
     3781 cattgccgga ttgccgtctg gtgaggctaa accgagcgga gagaggaaag cgtatagcgt
     3841 gaataagccc aacacgatag aagccactgc cagtggggca tattcccagc tggttaagtc
     3901 cacattggcg tggttgattt tgttgtcttc gtatggagtc gcacgtttta ggaaaattcc
     3961 taaaatcagc attaacgcca catcgaacac gaacaagcca gcttgcacat acacgaagtt
     4021 gatttttaca tcaaacaccc aaacgatgca gaagtagaga ataacgtgtg cgagtaaggc
     4081 aatgcgtgcg gctttgcctg agacggtttt gttgaataag ccgatgacaa acagtgccac
     4141 aatcggaatg ttcacaaaac cggcgaaacg tttggtcagc aagaaaatac cgtctgtgcc
     4201 gaacatcagt aacggcccaa agatcatcgt aatcaccgcc attaccgcag agactttttt
     4261 cgcataactt atcagttccg catctgtgaa ctgttttttg ctgatagacg gcagtaagtc
     4321 tttacagtaa atggttgccg ctgagtttaa gaacgagttg aaggtactca aaatcgcccc
     4381 gaacagtgcg gcaataaaga agccttgtag cacggttggg aaagttttat tcactaattc
     4441 agggtaagac acatcaatcg gtttcaagcc ctcacctaaa atatggaaac tgagtaagcc
     4501 cggtaagttt aagataagcg gtagcaataa taagaacagt gccgcaatta agatcccttt
     4561 ttgaccggct tttaagtctc tcgcccccaa ggcacgttgc acaatggctt ggttggtcgt
     4621 ccagtagaag aaatgcacca ccataatacc ggtgaaaatg gtcggccaag gcactgcatc
     4681 ggtggcacta cccaccgcat tccatttttc aacgtgagta gtggtgatga ttttcacccc
     4741 ttctgccata ctgccattac ctaaatagat aagtgcaaac accggcacta ataatgcccc
     4801 aatgactaac aagaccgcat tccaagtgtc tgacaccgcc accgctttta agccaccgaa
     4861 aatggcataa atcgccccaa caataccaat cgcaatcacc gtataggtaa tcgctgcccc
     4921 atagcttaag ccgaaggcag tttcaaggtt gaagatttta ttgaaggcaa tcgcaccggt
     4981 gtaaagtgca gtcggaatga cgatgaaaag atagaacacc aaaaacaagc ccgacataat
     5041 caaacgggtt tggcggtcga aacggttttc aaagaactcc ggaatggtgg tataaccgtt
     5101 gcggatatat ttcggcaaca gataaagtgc caagaagcaa agcggaatta ccgccggcac
     5161 agtccacgca ataattgaga aattaccggc ataagagttg gcattcacgc cgataagttg
     5221 ctcggtagaa agggaggtta agaccatcga acagccgata accgtagcac tcaagccacg
     5281 ccctgccaag aaataacctt tagatgtcga aagatcgtcg tttctggttt ttagccaaga
     5341 gatatacccg actaatccag ttacaaccag aaaggtaatt attgttaata acatattctt
     5401 cacctcaata tgactatata aatgatatat cccattagag acggcaatag cataaatctt
     5461 atttttaacg atagaatatg atgtttgtca taatagatat gtgatggtga tcacaactat
     5521 gaataattta tgacaattta actgattttc tttaattctc gccttaatcg aatggctgtt
     5581 tcggtttcac aacctgcaaa ttcggcgatt tcacggttag tccagtgata attgggataa
     5641 cgttggtgta aataatgaat actgttcatt acccgctctt gcgttttttg ataggaaacc
     5701 attgataaac gatgctctgc gtcttgtaat tctgtcgaga gctgttttaa cagagagata
     5761 attaaggcgg aattattcga ccaaaactca atgaaattta agggggtaat acgtatcaat
     5821 tctgccggct gtaacacccg agccgagcaa tggtagcgat tatcgccaaa aaaggttcta
     5881 aacccaaaat aatcgttggc tttatatacc ctcaccaagc tctctttacc attttcaagc
     5941 atatggtaca gccctattaa tccgcttttt aaaaaataaa attctctggc gtgataaccc
     6001 tgagaataaa tgacgactcc tttttctaga tcagacatta cacttaattg gcaagacatc
     6061 aattgttcgg gggttggcat attgttcatt gattatcctt attattttat tttttccata
     6121 tatgattgcg atcataatcc tttatttaga aaattttgca tacttatcac actttaggag
     6181 atggattatg acaatgaccg tgataattat tttgatcatt tgtggcatta tagtcaatat
     6241 cacttctgca ctatttggga ttggcggggg tgttttaatg atgccgattt tacattcact
     6301 gtttcctgaa ctgcctctcc aagtgatttc cgcctgctct cttaccattg tgattggttc
     6361 agccactatc aatttatgct tattccacca tcaacagata aaaatcaatc gaaaaaatgt
     6421 gttgatttgg tcacttgcga tgatgttagg cgtacaaatt gggtttgaac tcagttttct
     6481 tttctctgat aacagcatta tcgctatctt tattctaacc ctgctgacac tttcatttaa
     6541 aaccctattt cacaaaccaa gagttaaagc aatagtacca cctaaaccaa aagaagcagt
     6601 atctaatgcc acaatgtgca gtttaggcgg tttaattgca ggtattaccg gcattggcgg
     6661 cggttcgatt atggctccga ttgtcacgca aatacgctcg gtttcaccga aacaggttgc
     6721 agtttatact aactatatga tggtgattgg cggaatcggc aatttatatg gctacttatc
     6781 ccgccctttt gtgttgaatt caaccttagc aacttggcaa atcggctatg tgaattttgc
     6841 aattgtcggc accgtagtga taagttctct ctttactgtc tatttttcaa tgaaattacg
     6901 caatatcttg agtcccgaat taactactaa attacttggt gtaatcctat tcactattgc
     6961 ctgttatatg ggctttttgc aatatttatt cacttaacca taagttcaat agattattgc
     7021 ttatgaggta gtttttccca agtaacttca ttacgcaaat acaccggttc tatttcaaaa
     7081 gcagattgga tattgccttt ttgaatctca gtttcagcaa tcgaaagcat atatcggctg
     7141 gaaggcaagg taatatcact gataacaagc ggtagatttt gctcagaaaa ttgcgaatat
     7201 gccgcccaac ctgtgccaac caccacagtc tcttcactca tttggaattg acttattgcg
     7261 acttctggcg aacagacttg ttcagccacc acttcattcc attcttcacc ctttcgttca
     7321 aattgagcaa agtaaacttc attcattcgg gcatcaatta aggcaattac ctttgttgca
     7381 cctaattttt ggtatgcttc ttctgccatt gcttttaagt tagaaacagc taccaccggc
     7441 aaattcgctc ccattgccaa cccttgtgcc acacttacac cgaccctcac accggtaaaa
     7501 ctgcccggcc cacgtccgaa tactaaataa tttacttctt tgagttcgat attggcttga
     7561 ttaagtaatt catctaccat cggcaaaata cgttgagtat gggaacgagg gctgatttca
     7621 tctaagaacg tgatatttcc gttatactgt aacgctacag agcaagcctc tgttgcagta
     7681 tctaaagcaa gaatgctggg tttcatcata tgtttagttt gaataaatta aatttgcttt
     7741 aaggttaaac aaataaaggg cgaattatat tcgccctcgg ttcaagaaaa gattattttg
     7801 caaaatttct gcgcttttca accgcttcag caagtgcgaa taacgctttt tcactatctt
     7861 cccagccaat gcagccgtct gtaatacttt gtccgtaaac taacgcctct aaacctttgc
     7921 cttcaattaa gtcttggcga ccttctacca agtggctttc aatcatcaca cctgaaataa
     7981 agtctgatcc acctgcaatt tggttgcaca catcagcaca aacgtccatt tggcgtttga
     8041 actgcttctg gctgttagcg tggctgaaat cgatcatcac gtgtggacgt ccgccggatt
     8101 tctcaatcgc agcacaagcc gctgcaacgg agtcagcatc gtagttggtt gcgctatcgc
     8161 caccacgcaa aataatgtga cagtcaggat ttcctgctgt tgaaacaatc gcagaatgcc
     8221 cgaatttagt gaccgataag aaatggtgtg gcgattttgc ggaagaaatt gcatctaatg
     8281 caattttcac actaccattc gtgccgtttt taaaacctac cgcacaggat aaaccagagg
     8341 ctaattcacg gtggacttga gattcagtcg ttctcgcccc aattgctccc cagctcataa
     8401 agtcagccac atattgtggt gtaatcatat ccaaaaactc gccggcagtc ggcacgctta
     8461 aatcattaat atcagataat actttacggg caatgcgtaa accatcgttt aaggcaaagg
     8521 tttcgttcaa ataagggtcg ttaattaagc ctttccaacc aacggttgta cggggttttt
     8581 caaaatagac acgcatcacc acttctaagt tttggttaat tttcggatca gcacgcattg
     8641 ctttaatttt ttgtgcatat tctaatgccg ctttcgggtc gtgaatggag caaggtccaa
     8701 tcaccactaa taagcgatca tccgcaccgt gtaaaatttt gtggattgat tgacgggttt
     8761 tagcaacggt ttccgctgca acatcacttg ccggataggc ttccagtaat gcaataggtg
     8821 gtagtaattc ttcaatactg gtaattctga catcgtcatt tttgatataa gacattgtgt
     8881 tcactctcta aataaaatg
//
LOCUS       DANLYI010000094         8746 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.094, whole genome shotgun sequence.
ACCESSION   DANLYI010000094 DANLYI010000000
VERSION     DANLYI010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 8746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8746
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.094"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            complement(<1..325)
                     /locus_tag="PXD81_001523"
     CDS             complement(<1..325)
                     /locus_tag="PXD81_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="HDL5537538.1"
                     /translation="MPTKPHKEYHELVELLISRGMLINDKSYAEKKLSQVGYYRLSGF
                     WHISRKPNQDGGLSENFLENVQFEEVYKLYIFDKKLRLLLLDIIERLEVNIRNIMAHE
                     LGRENP"
     gene            complement(537..2654)
                     /gene="spoT"
                     /locus_tag="PXD81_001524"
     CDS             complement(537..2654)
                     /gene="spoT"
                     /locus_tag="PXD81_001524"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HDL5537539.1"
                     /translation="MYLFEPLDRIIQGYLPPEQIERVKRAYVIARDAHEGQTRSSGEP
                     YITHPVAVASIIAEMKLDQEAVIAALLHDVIEDTPYTEEELAAEFGQSVADIVQGVSK
                     LDKLKFRTRQEAEVANFRKMILAMTKDIRVVLIKLADRTHNMRTLGALRPDKRRRIAK
                     ETLEIYSPLAHRLGIEHIKNELEDLGFEAMHPQRYRVLKMAIASARGHRKELIQRIAD
                     DIRGRLEEVQIEARVYGREKHLYSIYQKMRQKDQHFHSILDIYAFRVVVDNVDNCYRV
                     LGQMHALYKPRPKRVKDYIAVPKPNGYQSLHTSMIGPHGVPVEVQIRTEDMDKMADMG
                     VTAHWRYKEGESHHNTTVQLKAQQWLQSIVELQNSAGNTAEFIDSVKSDLFSDDIFVF
                     TPKGRIVELPANATAIDFAYAVHSGIGDRCVGAMVDRNPYPLSEPLRTGQTVEIITEQ
                     GKRPNASWLNFVVSGKARAKIRQSLKNQQHDEAIALGRRQLVRAMNLRSLDELCSETI
                     QSVLDELRLSSFDELLAEIGLGNQMSNVIAQRLLGEPLLIDTDGNPENNQPLKIEGIE
                     NRLISFANCCLPIPGDEIIAHVSSGKGLAIHNACCGNVKDYATEPSNYLPVEWEIAKE
                     KAIQFETEIVLDIINEPGVLAGITSTLAKLNSNIGSINSEAREGNIYQVRVQISVENK
                     EHSELILRKLLSVKGVVKAARKQ"
     gene            complement(2764..3045)
                     /gene="rpoZ"
                     /locus_tag="PXD81_001525"
     CDS             complement(2764..3045)
                     /gene="rpoZ"
                     /locus_tag="PXD81_001525"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005613378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HDL5537540.1"
                     /translation="MARVTVQEAAEKIGNRFDLILIAARRARQLQLHTREPLVPEEND
                     KPTVIALREIEEGLITTDIMDQLENHDSIKQEAAEQEAISFLTEMSNEA"
     gene            complement(3148..3645)
                     /locus_tag="PXD81_001526"
     CDS             complement(3148..3645)
                     /locus_tag="PXD81_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HDL5537541.1"
                     /translation="MAHFYEYLEFNSDEDRENQLEVYVEVKLSTDTEEKLQAVGVQGD
                     WLVMAEPYCPDCVEIVAYFQRMAKLNPNIKVKYVARKDNQERKHFDSDEQQQAVISAQ
                     KIPTIFDIRNGKTELVLSEFPQFLKDKMAANPEQFEQLKADFRMGKFGKEVEAELLAI
                     LLNTK"
     gene            complement(3670..4692)
                     /locus_tag="PXD81_001527"
     CDS             complement(3670..4692)
                     /locus_tag="PXD81_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005825060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL5537542.1"
                     /translation="MSVTKKRLTLSDIAELSGVSKTTVSMILNGRGNDFRIKSETCQK
                     VKEIAKKHGYRANVYAKSLQAQRTNVIGLVIPDLTNYGFALTSRTLEKLCRENGLQLV
                     IACSDDTFEGEKKAIERLLDRQVDLLITAPTHNEPTYYQNAAQYTPMLQLDRYISGLN
                     IPSITSNDTQSISMLVENIVRAYHLKEFFYLGGQLSLSPSQNRLKGFREGLEQSHLAL
                     DENWVLHQDYQPESGYQMFAQIVEKLGRLPEAVFTASFTLLEGVMRYLTEHQQMDKLL
                     TQELHLATFDDHHLLNALPFHIHSIKQDHQQIAERTFELIQQKLAQQKIDDVKVDCEI
                     LWRKSI"
     gene            complement(4732..6162)
                     /locus_tag="PXD81_001528"
     CDS             complement(4732..6162)
                     /locus_tag="PXD81_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005603139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HDL5537543.1"
                     /translation="MHIFNNGKYKSLHAQSEGELNALRQTVLSDPDFYPTYHLAPETG
                     LFNDPNGLIFDGEKYHIFAQWFPYGALHGMKHWQHFITTDFQTFSKGDLLIPDELFES
                     HGCYSGGAILWQDKIVAFYTGNTRRPSDNARIPHQNIAIFDKSGKLLEKRCIIDQAPA
                     GYTEHVRDPKPYLTAEGKIRFVLGAQRENLTGTALIYEMDDLNATPRLIAELEVHNFD
                     NSNVFMWECPDLFSLGGKDLFVWSPQGKLRESHQFQNNYHATYALGELNGNRLRAEHI
                     EELDYGFDFYAPQTVENSDRILFGWVGLPDLTYPTDKYQWHSILTLPRKLSLQNGKVI
                     QNPIVNVGELQAVEMSENFAIENLDTAYLQISVENQPLVLDFFSNEQGQKLSLRFENG
                     VLSLDRSQTEQTELMEKFGSVRHCKIEQLETIEIFFDRSVVEIFVNGGEKVLTSRFFI
                     AKRENVVKSSRPIQVKVAKVLPISVE"
     gene            complement(6267..7190)
                     /locus_tag="PXD81_001529"
     CDS             complement(6267..7190)
                     /locus_tag="PXD81_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005606644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoimidazole riboside kinase"
                     /protein_id="HDL5537544.1"
                     /translation="MSKIWVTGDAVVDLIPDGEHHYLKCAGGAPANVAVGVSRLGVEA
                     GFIGRVGNDPLGKFMREVLQAENVCTKQMILDAQHRTSTVIVGLDNGERSFTFMVNPS
                     ADQFLEIGDLPEFNQGDFLHCCSIALINDPSRSTTIEGIRRVKKAGGYVSFDPNLRES
                     LWKSLDEMKTVVNSVVAMADILKFSEEELTLLTETETLEQATQAITAQYPEKLIIITL
                     GKDGAIYHFNGKSQVVAGKALKPVDTTGAGDAFVSGLLAGLAQTPNWHDETVLVEVIR
                     KANASGALATTAKGAMSALPNKAELDAFLAE"
     gene            complement(7307..8734)
                     /gene="miaB"
                     /locus_tag="PXD81_001530"
     CDS             complement(7307..8734)
                     /gene="miaB"
                     /locus_tag="PXD81_001530"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005617789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HDL5537545.1"
                     /translation="MAKLHITTWGCQMNEYDSSKMADLLNSTHGFELTENPEDADVLL
                     LNTCSIREKAQEKVFSQLGRWKNWKKDKPNLIIGVGGCVASQEGEHIRDRAPYVDIVF
                     GPQTLHRLPEMINQIRGGKSSIVDISFPEIEKFDRLPEPKAEGPTAFVSIMEGCNKYC
                     SFCVVPYTRGEEVSRPVDDVLFEIAQLAAQGVREVNLLGQNVNAYRGETFDGGICTFA
                     ELLRLVAAIDGIDRLRYTTSHPIEFTDDIIEVYRDTPELVSFLHLPIQSGSDRVLTMM
                     KRNHTALEYKAIIRKLRAVRPEIQISSDFIVGFPGETAEDFEQTMKVIEQVNFDMSFS
                     FIYSARPGTPAADLPDNVSDDEKKERLSRLQQRINHQAMQFSRAMLGTEQRILVEGPS
                     KKDLMELTGRTETNRVVNFVGTPDMIGKFVDVKITDVYTNSLRGEVIRTEDQMGLRVV
                     ESAESVIARTRKEDDLGVGKYVVNL"
BASE COUNT         2395 a         1997 c         1701 g         2653 t
ORIGIN      
        1 aaggattttc acgccccaat tcatgagcca taatattacg aatattaact tctaaacgtt
       61 caataatatc caataataaa agccttagtt ttttatcaaa aatatatagc ttataaactt
      121 cttcaaattg tacattttcg agaaaatttt cgctaagccc accatcttga ttaggctttc
      181 tagatatatg ccaaaatcca gataaacgat aataccctac ctgagataat tttttctctg
      241 catagctttt atcatttatg agcattcctc tagatatgag tagttctact aattcgtgat
      301 actctttatg tggttttgta ggcataaaat tgaaggcatt ttaagaaagg caaggacgta
      361 gcttgacact ctaagtcgat tacatttagt aaacctcagg gacaggggct acgccacgat
      421 tgcaagggat tatacatttt agctgattaa agtcaaaagg tttcatcaat taataatagc
      481 aatactgtac ttttaaggac tataattgca aaaaactgat aaaattttac cgcttgttac
      541 tgcttacggg ccgcttttac aacacctttt acacttaata gcttacggag aatcagctca
      601 gaatgctctt tattttccac agaaatttgt actctcactt gatagatatt tccttctctt
      661 gcctcgctat taatgctgcc aatgttgcta tttaatttgg caagcgttga tgtgatcccc
      721 gcgagtaccc ctggttcatt gataatatcc agtacgattt ccgtttcaaa ttgaatcgct
      781 ttttctttgg caatttccca ttccacaggt aaataattac tcggctctgt tgcgtaatct
      841 ttcacattac cacaacaagc attgtggatt gccaatcctt tacctgaact gacgtgagca
      901 ataatttcat cacccggtat aggtaagcaa cagtttgcaa aactgataag gcggttctca
      961 atcccttcaa ttttaagcgg ttgattattt tctggattac catcagtatc aattaatagt
     1021 ggctccccta ataagcgttg agcgataaca ttactcattt ggttacctag accaatttca
     1081 gccagtagct catcaaagct tgataaacgt agctcgtcta atacggattg aatagtttct
     1141 gaacacaatt catcaagtga gcgtaaattc attgcccgaa ctagctgtct acgcccaagt
     1201 gcaatcgcct catcgtgctg ctgattttta agcgattgac gaattttagc cctcgcctta
     1261 ccacttacta caaaatttaa ccacgatgca tttgggcgct taccttgctc ggtaataatt
     1321 tccaccgtct gtcctgttcg tagcggttct gaaagtggat acggattgcg atcaaccatc
     1381 gcacctacac aacgatcccc aatgcctgaa tgcaccgcat aggcaaagtc aatagcggta
     1441 gcattcgccg gcaattcaac aatacgccct ttcggggtaa agacgaaaat atcgtcagag
     1501 aacagatccg atttaacgct atcaataaat tctgctgtat tgccggcact gttttgcaat
     1561 tcaacaatac tttgtagcca ttgctgggct ttgagttgaa cggtcgtgtt atggtgcgac
     1621 tcgccctctt tgtaacgcca gtgagctgtt acccccatat ccgccatttt gtccatatct
     1681 tcggtgcgaa tctgtacctc caccggtaca ccgtgcgggc caatcatcga agtgtgtaaa
     1741 gattgatagc cgttcggttt tggcacggca atataatctt tcacccgttt cggacgaggc
     1801 ttatacagtg cgtgcatctg ccctaataca cggtaacaat tatctacatt atcaaccact
     1861 actcggaaag cataaatatc taaaatcgaa tgaaaatgtt gatctttctg gcgcattttt
     1921 tggtaaatgg aatagagatg tttttcacgc ccatacacac gggcttcaat ttgcacctct
     1981 tctaaccgac cacgaatatc atcggcaatg cgttgaatta gctctttacg atgtcctctg
     2041 gcactggcaa tagccatttt cagtacccga taacgttgcg ggtgcattgc ctcgaagcct
     2101 aaatcttcca actcgttttt aatatgctct attcccaagc gatgtgccaa cgggctataa
     2161 atctctaagg tttctttcgc tatacggcgg cgtttatccg gacgcaaagc accaagcgta
     2221 cgcatattat gggttcggtc ggcaagttta attaacacaa cccgaatgtc tttggtcatt
     2281 gccaaaatca ttttacggaa gtttgccact tctgcctctt ggcgggtgcg gaattttaat
     2341 ttatccagct tagaaacgcc ttgtacaata tctgctacgc tctgtccaaa ttccgccgcc
     2401 agttcttctt cggtgtaagg tgtatcttca atcacatcgt gcaacaaagc cgcaatcacc
     2461 gcttcttgat cgagcttcat ttccgcaata atcgacgcca ctgctaccgg gtgagtaata
     2521 tagggctcac cgctagagcg tgtttgtccc tcgtgagcat cacgggctat cacataagca
     2581 cgttttacac gctcaatctg ctcgggtggc aaatagcctt gaataattcg atctaaaggt
     2641 tcaaaaagat acacgttata cctcaacaac agaaataaaa atggacaacg aatttgcaaa
     2701 ttttgaggaa aatcctaccg cttgcagact caattgtcca ttctctattt acgaaaaata
     2761 aaattacgct tcgttgctca tttcagttaa aaatgaaatc gcctcttgct ccgccgcttc
     2821 ttgcttaata gagtcgtgat tttccagctg atccataatg tcagttgtaa ttaaaccttc
     2881 ttcaatttca cgtaatgcaa tcacggtcgg cttatcgttt tcttcaggca caagtggctc
     2941 acgggtgtga agttgtaatt gtcttgcacg acgtgcagca attaagatta aatcaaaacg
     3001 gttgccaatt ttctccgctg cttcttgtac ggttacacga gccataaatt tactccaaag
     3061 atttattcgg ttttattcat aaaaagattg cgaattatac agaaattccg ccaattccgc
     3121 aatgaaagcg ggctaaattc acaaaattta tttggtattc agtaaaatcg ccaataattc
     3181 agcctcaacc tctttgccga attttcccat tctaaaatcg gcttttagtt gctcgaattg
     3241 ctcagggttc gctgccattt tatcttttaa aaattgcgga aattcagata acactaattc
     3301 tgttttgcca ttacgaatat caaaaatagt tggaattttt tgcgcagaaa tgaccgcttg
     3361 ttgctgttca tcgctgtcaa aatgtttgcg ttcctgatta tctttgcgag ccacatattt
     3421 caccttaatg ttaggattga gctttgccat tcgctggaaa taagccacaa tttccacaca
     3481 atcggggcaa tatggctctg ccattaccag ccagtcccct tgcacgccaa cagcttgcag
     3541 tttttcttcc gtatctgtgg aaagcttgac ctccacatac acttctaatt gattttcacg
     3601 atcttcatcg ctgttaaatt ctaaatattc gtaaaaatgt gccataattt tgtcttttcc
     3661 tatctcatct taaatggatt tacgccataa aatttcacaa tcaactttaa cgtcatctat
     3721 tttctgttgc gctaactttt gctgaattaa ttcaaaggta cgttcggcaa tttgttggtg
     3781 gtcttgctta attgaatgaa tatgaaaagg tagtgcattc agtaaatggt gatcatcaaa
     3841 ggttgctaaa tgcagttctt gagtcaataa tttatccatt tgttggtgtt cagtcagata
     3901 gcgcattact ccctcaagta gggtaaaaga ggctgtgaat accgcttctg ggagcctgcc
     3961 aagtttttca actatctgag cgaacatttg atagcccgat tccggctgat agtcttgatg
     4021 cagaacccaa ttttcatcaa gtgccaaatg tgactgctcc aaaccttcac ggaagccttt
     4081 taaacgattt tgacttggcg agagtgaaag ttgtccacct aagtaaaaaa actcttttaa
     4141 gtgataagcc cgtacaatat tttccaccaa catactgata ctttgtgtat cattactggt
     4201 gatagaaggg atattcaacc cagaaatata gcggtctagc tgtaacattg gggtatattg
     4261 tgccgcattt tgataataag tcggctcatt atgtgtcgga gcagtaatca gtaaatctac
     4321 ctgtctgtct aataaacgct ctatcgcttt cttctcacct tcaaaggtat catccgagca
     4381 agcgatcact agctgtaacc cgttttcacg gcataatttc tctaaggttc ttgaggtcag
     4441 agcaaagcca taattcgtta aatcgggaat gactaaaccg atcacattcg tgcgttgagc
     4501 ctgtaatgat tttgcatata cattagcacg ataaccgtgt tttttagcga tctctttcac
     4561 tttttggcag gtttcggatt tgattctaaa atcattgccc cgcccattta aaatcatact
     4621 gactgttgtt ttagatacgc cgctgagttc cgcaatatca ctgagcgtta ggcgtttttt
     4681 agtcacactt atctcctttt agcataaaaa tgggcattac tgcccattgc tttattctac
     4741 gctaattggc aatacttttg ctactttcac ttgaatcgga cgagagcttt ttacgacatt
     4801 ttcacgctta gcgataaaaa agcgtgaagt cagcactttt tccccgccgt ttacgaaaat
     4861 ttccaccaca gaacggtcaa agaaaatctc gattgtctct aattgttcaa ttttgcaatg
     4921 acgaaccgag ccaaattttt ccatcaattc tgtttgttcg gtttggctac gatctaagct
     4981 caacacccca ttttcaaaac gtaagctgag cttttgcccc tgttcattgc tgaaaaaatc
     5041 caacacaagc ggttgatttt ccacagaaat ttgcaagtag gcagtatcca aattctcgat
     5101 tgcaaaattt tctgacattt cgaccgcttg taattcgcct acattcacga ttgggttttg
     5161 tatcactttg ccgttttgca gggaaagttt acgaggcaat gtcagtattg aatgccactg
     5221 atatttatca gttgggtaag tgagatcagg caagccgacc cagccaaaca aaatgcgatc
     5281 ggaattttcc accgtttgcg gtgcgtaaaa gtcaaagccg tagtccagct cttcaatatg
     5341 ctctgcccgt aatctgttgc cgtttagctc gccaagtgcg taagtggcgt ggtagttatt
     5401 ttggaattga tggctttcac gcagtttgcc ttgtggcgac cacacaaaca gatctttacc
     5461 accaagtgag aacagatccg gacactccca cataaacaca ttgctgttat caaaattgtg
     5521 gacttccagt tctgcgatta aacggggtgt ggcatttaaa tcgtccattt cataaattaa
     5581 cgccgtgccg gtcaagtttt cacgctgagc ccccaacaca aaacgaattt tgccctctgc
     5641 ggtaaggtaa ggtttcgggt cacgcacgtg ttcggtgtag cctgccggtg cttggtcgat
     5701 aatgcaacgc ttttccaata atttgcccga tttatcaaaa atcgcgatat tttggtgagg
     5761 aatgcgggca ttatctgatg ggcggcgtgt attgccggtg taaaaagcga caatcttatc
     5821 ttgccataaa atcgccccac ctgaataaca gccgtgggat tcaaacagct catcgggaat
     5881 cagcaaatcc cctttactga acgtttgaaa atcggtagtg atgaaatgct gccaatgttt
     5941 cattccatgt aatgccccat aaggaaacca ttgggcgaaa atatggtatt tttcaccatc
     6001 gaaaatcaag ccgtttgggt cattaaataa gcctgtttcg ggagcgagat gataggttgg
     6061 gtaaaaatcg ggatcagata aaacggtttg gcgaagagcg tttaattcgc cctcgctttg
     6121 tgcgtgcagg cttttgtatt tgccgttgtt gaaaatgtgc atagagtgtt cctgtcattt
     6181 cagaaattac gatggtgcaa gcgtccacgc ttgtaccctt ttgtaaaaaa tgaggcacaa
     6241 gcgtggacac ttgcgccatc attacattat tccgccaaaa atgcgtctaa ctccgcttta
     6301 ttcggtaatg ccgacatcgc cccttttgct gtggtggcta acgcaccgga agcatttgct
     6361 ttgcggatca cttcaactaa cacagtttca tcgtgccaat tcggcgtttg agcaagaccg
     6421 gctaataatc cgctgacaaa ggcatctccg gcaccggtgg tatccacagg tttcaaggct
     6481 ttgccggcaa cgacttggct ttttccgttg aaatggtaga ttgcaccgtc tttgccaagg
     6541 gtgataataa tcaatttctc cggatactga gcggtaatag cttgtgtggc ttgttccaaa
     6601 gtttctgtct cggttaataa ggtcagctct tcttcggaaa atttgagaat atccgccatc
     6661 gccaccacag aatttaccac tgttttcatt tcatcaaggc tcttccagag cgattcacgc
     6721 aagtttggat caaacgaaac atagccaccc gcctttttca ctcggcgaat cccttcaatg
     6781 gtagtggaac gggatgggtc gttaatcaag gcaatagaac agcagtgtaa gaaatcacct
     6841 tggttaaatt caggtaaatc gccgatttct aaaaattggt cggcactcgg atttaccata
     6901 aaagtaaaac tacgctcacc gttatctaag ccaacaatca cggtggaagt gcggtgctga
     6961 gcgtctaaaa tcatctgttt ggtgcaaaca ttttctgcct gtaacacttc tcgcataaat
     7021 ttacccagag ggtcgttacc cacacgcccg ataaatcctg cctcaacccc taaacgagaa
     7081 acgcctactg caacattcgc cggagcacca ccggcacatt ttaaatagtg atgttcgccg
     7141 tcgggaatta aatccaccac cgcatcgcct gttacccaaa tcttactcat ctcatacacc
     7201 tttaattagt cacaaaacac caagctaaaa cgatttagca tttattcgat tatatactaa
     7261 aagaccgctt gtacatacct acaagcggtc attttttctt taatttttac agattaacca
     7321 cgtatttacc tacacctaaa tcatcttctt tacgagtgcg ggcaatcacg ctttctgccg
     7381 attccaccac acgtaatccc atttggtctt cggtacgaat cacttcgcca cggagtgagt
     7441 tggtataaac atcggtgatt ttcacatcga caaatttgcc aatcatatcc ggtgtgccaa
     7501 caaagtttac tacacggtta gtttcggttc tgccggttaa ttccatcaag tctttcttag
     7561 acgggccttc aaccaaaatg cgttgttctg taccgagcat tgcacggcta aattgcattg
     7621 cttggtggtt gatgcgttgt tgtaaacggg ataatcgctc ttttttctca tcatcggaga
     7681 cattatcagg cagatctgcc gctggtgtac ccggtcgagc cgagtagata aagctaaagc
     7741 tcatatcaaa gttcacttgc tcgatcactt tcattgtttg ctcgaaatcc tctgcggttt
     7801 cgcccgggaa gcctacaata aagtccgaac taatttgaat ctcaggtcga acagcacgca
     7861 atttacggat aatcgctttg tattccaacg ctgtgtggtt acgcttcatc attgttaata
     7921 cacggtctga accactttga atcggtaaat gtaagaaact gaccaattca ggtgtatcac
     7981 gataaacttc gataatatca tcggtaaatt caatcgggtg gctggtggtg taacgtaagc
     8041 ggtcaatacc gtcaatcgct gccactaagc gtaataattc ggcaaaggta caaatgccgc
     8101 catcaaaagt ttcaccacgg taagcattta cgttttgacc gagtaaatta acctcacgca
     8161 caccttgtgc cgcaagttgg gcgatttcaa ataatacgtc atccaccggg cgggaaacct
     8221 cttcgccacg agtgtaaggc acaacgcaga acgaacaata tttattgcag ccttccataa
     8281 ttgaaacgaa tgctgtaggg ccttcggctt ttggctccgg caagcggtca aatttttcaa
     8341 tttccgggaa agaaatatcg acaatcgagc ttttaccgcc acggatttgg ttgatcattt
     8401 caggcaaacg gtgcaaagtt tgtggcccga aaacaatatc tacataaggg gcacgatcac
     8461 ggatatgctc cccttcttgt gaagccacac aaccgccaac accgataatc aggttcggtt
     8521 tatctttttt ccagtttttc caacgcccga gttgcgagaa tactttttct tgtgcttttt
     8581 cacggataga acaagtgttt agcagcaaca cgtctgcatc ttcagggttt tcagtcagtt
     8641 caaaaccgtg agtagagttt aggagatccg ccatttttga tgagtcgtac tcattcattt
     8701 ggcagcccca agtggtaata tgtaatttag ccataacgtt taaaat
//
LOCUS       DANLYI010000095         8695 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.095, whole genome shotgun sequence.
ACCESSION   DANLYI010000095 DANLYI010000000
VERSION     DANLYI010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 8695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8695
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.095"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            241..429
                     /locus_tag="PXD81_001531"
     CDS             241..429
                     /locus_tag="PXD81_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011200812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5537546.1"
                     /translation="MSYDANDALNEIEEALSELERVAEDLINNNPNKESELRGQGVHQ
                     ATKHLRFRIRNIRRGEAI"
     gene            470..772
                     /locus_tag="PXD81_001532"
     CDS             470..772
                     /locus_tag="PXD81_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011200813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5537547.1"
                     /translation="MKIKLYFPDESIATIKRMGLRQMSPETLRWTADHPSSSYGMGAL
                     LRGKSGEILDGKSFAAMVHAFGAWIETDSEDTSRRVHNALVTAATGTEESVKVAKE"
     gene            772..2082
                     /locus_tag="PXD81_001533"
     CDS             772..2082
                     /locus_tag="PXD81_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011200814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HDL5537548.1"
                     /translation="MSNILNWPDYKVLQVSELEHDYQVHAEVSEPPTQCPHCNHPEIV
                     GFGRRDEVIMDTPVHGRRTGIMLNRRRYRCQSCRKTFLEPVPHKDEKRQMTNRLIQYI
                     ERESLRRTFSSVAEDVGVDEKTVRNIFNDYCERLEKTLNFEMPQWLGIDEIHIIKPRC
                     VITNIQQQTIVDMLDNRNKTTVTRYLSKRTDRDLVRYVAMDMWRPYRQAVETMIPDAT
                     VIIDKFHVVRMANESLERARKAIRSALTPQQRRGLMRDRFVLLKRRHELTDAEYMRFS
                     GWTLNYPEIGQAYELKEAFFEIWDCQTRHQAQEAYYSWLRQITPEMKAHYDPLIKAMG
                     NWHDDIFAYFDHPITNAYTESLNNLIRVVNRVGRGYSFEALRAKILFTEGFQKIKKPR
                     YQRQRIPEGAMGRMPFYGVAEAGPSTNYGADISTLVREIEAGRL"
     gene            complement(2178..3380)
                     /gene="tet(H)"
                     /locus_tag="PXD81_001534"
     CDS             complement(2178..3380)
                     /gene="tet(H)"
                     /locus_tag="PXD81_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(H)"
                     /protein_id="HDL5537549.1"
                     /translation="MNKSIIIILLITVLDAIGIGLIMPVLPTLLNEFVSENSLATHYG
                     VLLALYATMQVIFAPILGRLSDKYGRKPILLFSLLGAALDYLLMAFSTTLWMLYIGRI
                     IAGITGATGAVCASAMSDVTPAKNRTRYFGFLGGAFGVGLIIGPMLGGLLGDISAHMP
                     FIFAAISHSILLILSLLFFRETQKREALVANRTPENQTASNTVTVFFKKSLYFWLATY
                     FIIQLIGQIPATIWVLFTQYRFDWNTTSIGMSLAVLGVLNIFFQAIVAGKLAQKWGEK
                     TTIMISMSIDMMGCLLLAWIGHVWVILPALICLAAGGMGQPALQGYLSKSVDDNAQGK
                     LQGTLVSLTNITGIIGPLLFAFIYSYSVAYWDGLLWLMGAILYAMLLITAYFHQRKTT
                     PKAVISTP"
     gene            3472..4095
                     /gene="tetR(H)"
                     /locus_tag="PXD81_001535"
     CDS             3472..4095
                     /gene="tetR(H)"
                     /locus_tag="PXD81_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(H)"
                     /protein_id="HDL5537550.1"
                     /translation="MAKLDKEQVIDNALILLNEVGIEGLTTRKLAQKIGVEQPTLYWH
                     VKNKRALLDALAETILQKHHHHVLPLPNETWQDFLRNNAKSFRQALLMYRDGGKIHAG
                     TRPSESQFETSEQQLQFLCDAGFSLSQAVYALSSIAHFTLGSVLETQEHQESQKEREK
                     VETDTVAYPPLLTQAVAIMDSDNGDAAFLFVLDVMISGLETVLKSAK"
     gene            complement(4098..4616)
                     /locus_tag="PXD81_001536"
     CDS             complement(4098..4616)
                     /locus_tag="PXD81_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5537551.1"
                     /translation="MPFKTVTFAQATTNDGQSLLAVNDLFIGPKSHTSAQYILQWNGA
                     EEVQSSSGIIVSTGLGSTGWFQSILAGAMAITGEASHPLLQGFSWSDRKLQFSVREPF
                     PSRTTGVALTFGTIEPDSPLQLGSLMPENGVIFSDGIEDDYLQFNAGCIAHIGIADIQ
                     GQLISQKGRQRI"
     gene            complement(4665..4844)
                     /locus_tag="PXD81_001537"
     CDS             complement(4665..4844)
                     /locus_tag="PXD81_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5537552.1"
                     /translation="MFLVNQLLAITIATSAVLTNAQLAILAVAFWIAILPCARLATAV
                     GVWVFDDVVHNNSPN"
     gene            4933..5088
                     /locus_tag="PXD81_001538"
     CDS             4933..5088
                     /locus_tag="PXD81_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012340841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HDL5537553.1"
                     /translation="MGLNRAEMMYREGAYVIDPVFPATLGYAGAGVVVAVGDDAGEFQ
                     IGDKVSV"
     gene            complement(5178..6725)
                     /locus_tag="PXD81_001539"
     CDS             complement(5178..6725)
                     /locus_tag="PXD81_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012340840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase family protein"
                     /protein_id="HDL5537554.1"
                     /translation="MKRRDFLRYGIGISLASSSLYSLANMMNHAQHGNMAMMHSVPTG
                     LMPTEAMPSGLSLNGILPKLANQSTQAGLFKATLKAEPIKIRLADNKETEFWAYNGQL
                     PGPQIEVFEGDTVEIEFINHLPQPSTVHWHGLDVPNEADGNPMDMVEPQGKKVYRFTL
                     PQGSAGTYWYHPHPHDHVSEQVYKGLAGTFVVKAKNDPLAHLPEQHWVISDLRLNADG
                     TIPANTMLDWINGREGEFVLINGQYQPQIQVKTNERIRLWNATSARYFRLNIPGVKWI
                     VVGTEGGLLEKPRSPVDELLLTPAERVEVIMVGETQGTVNLQSGYYDRRKMMVQEQPK
                     DLTLATIQVKSEPVQLPESLRSLPNWDEPKQVRQIRFSEKMMNHTNMPMMNHGTHHAT
                     TTLTDNPIPPMMNGMFLINGQTFDMNRIDFVTKLNEVEQWEIFNESHMDHPFHLHGTQ
                     FEVIQHTLNGKTFKPEGRALKDVVNLRPYEKVIIRFKQGHTGLKMYHCHILEHENLGM
                     MGMFKVE"
     gene            complement(6739..7194)
                     /locus_tag="PXD81_001540"
     CDS             complement(6739..7194)
                     /locus_tag="PXD81_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005819327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF411 domain-containing protein"
                     /protein_id="HDL5537555.1"
                     /translation="MKLHKFTRSLLLLSLGITAFAQAQTLSMEVWKSPTCGCCNEWIS
                     YMQKNGFEVKVNETGNYDAHKRFNIKYEHASCHTAVIDGYVIEGHVPAEDIKRLLAEK
                     PDAIGLSAPGMPIGSPGMDGEAYGGRKDPYDVVLIKKNGESEVFKSYNQ"
     gene            complement(7376..7696)
                     /locus_tag="PXD81_001541"
     CDS             complement(7376..7696)
                     /locus_tag="PXD81_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5537556.1"
                     /translation="MELKVIHKNLVDSAKIKLHAVYLVIFLFVSHFAMIFGMADPISA
                     GYQPTTPAMTHSMQMDAKQITEMHNQMMTGEITPEEMMKMHQEMNPNMNMPNTQGGHS
                     EHHK"
     gene            7695..8462
                     /locus_tag="PXD81_001542"
     CDS             7695..8462
                     /locus_tag="PXD81_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012340839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDL5537557.1"
                     /translation="MSKIIKIGKVANNSDAIDAISTQPRHSVIVGTIKRLQTDRIDLL
                     YQHRVDPNVPIEEVAGAIKDLMKQGKVLNWGLSEAGEKTIRKAHAIQPLTAVQSEYSL
                     FFRGREKDVILVCEELGIGFVPWSPLAMGALAGYVDENSRFEADPSKDLRGIIPRFTP
                     EAMQQNVKLIQLVQKWAKRKLCTPAQLSLAWLMAQKAFIAPIPGTSKARHLLDNLGAD
                     DVKFTEEELAQFRQELEAIEIVGLRLPEAILNFSEAK"
BASE COUNT         2516 a         1855 c         1911 g         2413 t
ORIGIN      
        1 ggttctcttt ttttacatta atttcgacac aacatcttta atttgatagt atttatatat
       61 gatattttgg gtatccggat ttagcggttg atctgcgcta gacgtaattt tgtaaataaa
      121 ccggatttta gggttgacgg aaaaggccag atgccctaat attagattca agagctgaga
      181 acaacggctc gggcataagc ccaacgggcg caccgccgaa cttggtgcag gatagaagcc
      241 atgtcatacg atgcaaatga tgcgctgaat gaaatcgaag aggcgcttag cgagcttgaa
      301 agggttgctg aagacctaat caacaacaac ccgaataaag aatcagaatt acgcggccaa
      361 ggggttcacc aagcgacaaa gcacctccgt tttcgtattc gcaacatccg ccgcggcgaa
      421 gcaatttgac taacatgccg ccctaggggc ggctttcagc gaggtcagca tgaaaatcaa
      481 actctatttt ccggacgagt ctatcgccac gataaagcga atggggcttc gccaaatgtc
      541 tcccgagact ctcaggtgga ctgccgatca tccgtcaagc agctatggta tgggcgcttt
      601 gctcagaggc aagtctggtg aaatccttga tgggaagtct tttgctgcaa tggtgcatgc
      661 ctttggcgcg tggattgaaa cagacagtga agataccagt cgtcgagttc ataacgcgct
      721 agtcactgct gccactggca ccgaggaatc ggttaaggtg gcaaaggagt aatgtcgaat
      781 atcctcaact ggcctgatta caaagtgctc caggtatccg aactggagca cgattaccag
      841 gtacacgccg aagtttccga accgcctacg cagtgccccc actgcaatca tcctgaaatc
      901 gtcggctttg gtcgccgtga tgaggtgatc atggatacac ccgtccacgg aaggcgtacc
      961 ggcatcatgc ttaaccgccg tcgctatcgc tgccagtctt gccgaaagac gtttctggag
     1021 cctgtaccgc acaaagacga aaagcggcag atgaccaacc gcctgatcca gtacatcgag
     1081 cgcgagagcc tgcgccgcac gttctccagt gttgccgagg atgtgggcgt cgatgaaaag
     1141 accgtccgta acattttcaa cgactattgt gaacgcctcg aaaagacgct caattttgaa
     1201 atgccgcagt ggttgggtat cgacgaaatc cacatcatca aaccccgctg cgtgatcacc
     1261 aacattcagc agcagaccat tgtggacatg ctggataacc gcaataaaac caccgtcacc
     1321 cgctacctga gcaaacgcac tgaccgcgac ctggtgcgct acgtggcaat ggatatgtgg
     1381 aggccgtacc ggcaagcagt agaaaccatg attcctgacg ccacggtgat catcgacaag
     1441 ttccacgttg tcaggatggc gaacgaatca ctggaacgcg ccagaaaagc catcagaagc
     1501 gccctgacgc cacagcagcg ccgtgggttg atgcgagata ggtttgtcct actgaaacgc
     1561 cgtcacgagc tgacagatgc cgaatacatg cgtttttcag gctggacatt gaattaccct
     1621 gagattggcc aagcctacga actgaaagaa gcctttttcg agatttggga ctgtcagacc
     1681 cggcaccagg cacaggaggc atattactct tggctgcgac agattacgcc ggaaatgaaa
     1741 gcccactatg acccgcttat caaagcgatg ggcaactggc acgatgacat tttcgcttac
     1801 ttcgaccacc ccattaccaa cgcctacacc gagtcactga acaacctgat tcgtgttgtt
     1861 aatcgtgttg gccgtggcta cagctttgaa gcgctgcgag ccaagatcct gtttactgag
     1921 ggcttccaga agatcaagaa gccacgctat cagcgtcaac ggatacccga gggcgctatg
     1981 ggcaggatgc cgttctatgg cgttgccgaa gcggggccat ccactaacta cggcgctgac
     2041 atatcaacac tggtgcggga gattgaggcg gggcgtttat agccctgttt caaccctgta
     2101 atccggatac ccaaataaag tcctatcaac gttattaaga gcggacagga cttttatgat
     2161 ctttataatt tctagtatta aggggttgaa ataacagctt taggtgtggt ttttctttgg
     2221 tgaaaataag cggtaataag caacatagca taaagtattg cccccatcag ccataacaga
     2281 ccatcccaat aagcgacgct ataactataa ataaaggcaa ataaaagggg accaatgatc
     2341 ccggtaatat tggttaggct caccagagta ccttgtaatt tcccttgcgc attatcatcg
     2401 acagattttg ataaataacc ttgtaatgcg ggttgcccca tacctcctgc cgctaagcaa
     2461 attaatgctg gtaagatgac ccaaacgtgg cctatccacg ctaataataa acagcccatc
     2521 atatcaatag acatactgat cataatggtg gttttttcgc cccatttttg tgccaatttc
     2581 ccagcgacaa tcgcctgaaa gaaaatattt aatacaccca gaaccgccaa agacataccg
     2641 atagaagttg tgttccaatc aaaacgatat tgtgtaaaca gcacccagat ggtggcagga
     2701 atttgcccga taagctggat aataaaatag gttgctaacc aaaagtagag gcttttctta
     2761 aaaaaaacag tgactgtatt tgaggcagtt tggttttcag gtgtcctatt ggcaacaagc
     2821 gcttctcttt tttgtgtttc tcggaaaaag agcaaagaga gtattaataa tatcgagtgt
     2881 gaaatagcgg caaaaataaa tggcatatga gcactgatat cacctaataa tccccctagc
     2941 attgggccga taataaggcc aacaccaaaa gcaccaccta agaaaccaaa atagcgagtt
     3001 cgatttttag cgggagtcac atcactcatc gctgatgcac atacggcacc tgttgcgcct
     3061 gtgatccccg caatgatgcg cccaatatag agcatccaaa gtgtggttga gaatgccatt
     3121 aaaagatagt cgagtgccgc gcctaaaagg gaaaacagca agatgggttt tctgccgtat
     3181 ttatcagaca gtcgtcctag aataggagca aaaataacct gcatggtagc atagagcgct
     3241 aatagcacac cgtaatgggt tgccagtgaa ttttcactga caaattcatt taatagagta
     3301 gggagtactg gcatgataag cccgatacca atggcatcta atacggtgat cagcagtata
     3361 ataataattg atttattcat tgagatccta aaaatctatc agtgatagag tgggtgtaat
     3421 atatctctat cagtgataga ttgtcaaggc tattttattt tgaggtaggt aatggcaaag
     3481 ctagataaag aacaagttat tgataatgcg ttgattttac ttaatgaagt tggtattgaa
     3541 ggattaacaa cgcgtaagct ggcgcaaaaa ataggtgtgg aacaacccac attgtattgg
     3601 catgtaaaaa ataaacgcgc tttgttagat gcattagcag aaactatttt gcaaaagcac
     3661 catcatcatg ttttgccatt gccgaatgaa acatggcagg actttttgcg aaataacgcg
     3721 aaaagcttcc gccaagcctt attaatgtat cgtgatggtg gcaaaattca tgcgggaaca
     3781 cgcccctctg aaagtcaatt tgagacatca gaacagcaac tacagttttt gtgtgatgct
     3841 gggtttagtc tatctcaagc cgtgtatgca ttaagctcta ttgcgcattt tacattaggc
     3901 tccgtactgg aaactcaaga gcatcaagaa agccaaaaag agcgtgaaaa agtagagacg
     3961 gatactgttg cctatccgcc attattaacc caagccgttg caattatgga tagtgataat
     4021 ggtgatgctg catttttgtt tgtccttgat gtgatgatct ctggacttga aacagtatta
     4081 aagagcgcta aataaatcta aattcgctga cgcccttttt ggctaattag ttgcccttgt
     4141 atgtcagcga taccaatgtg agcaatacaa cctgcattaa attgtaaata gtcatcttcg
     4201 atgccatcag agaaaattac gccattctct ggcatcaaag atcctaattg cagtggtgag
     4261 tcaggctcaa tagtgccaaa agtcagtgca acacctgttg ttctacttgg aaatggctct
     4321 cttacactaa attgtagctt tcgatcactc cagctaaagc cttgtaatag agggtgcgaa
     4381 gcttctcctg taattgccat cgcaccagca agaatagatt gaaaccaccc cgttgatccc
     4441 aatcctgttg atacaataat gcctgatgaa gattgcactt cttcagcgcc attccattgc
     4501 aaaatatact gtgcggaagt gtggctttta gggccaataa ataagtcatt aaccgctaat
     4561 aaggattgac catcattggt tgttgcttgt gcaaaagtga ccgttttaaa tggcattttt
     4621 ttattaatgg gtttaaaaac ggtttttgtt ttgataatca acctttaatt tggagaattg
     4681 ttatgaacga catcatcaaa aacccaaacg cccactgcgg ttgccaatct tgcacagggc
     4741 aaaattgcaa tccaaaatgc aactgccaaa atggcgagct gtgcatttgt caaaactgca
     4801 cttgtggcga ttgtgattgc aagtaattga ttaactaaaa acattgggcg ggcttaggtt
     4861 tgcccaatgt ttgccaaatc ccattttgat aaggaaaact ataatgaaaa tgtttaaaaa
     4921 ccgtgccgtg cattgggact aaaccgtgcc gagatgatgt atcgggaagg ggcgtatgtg
     4981 attgaccctg tgttccctgc gactttgggt tatgcagggg caggcgtggt cgtggcagtg
     5041 ggcgatgacg ctggcgaatt tcaaatcggc gacaaagtga gcgtctgatc tgcaccccaa
     5101 aagttggact aaaaaccaac tgattaaggt gcagattttt tatgacgaaa tccctctatt
     5161 tttatagtta gataccacta ttccacttta aacatcccca tcataccgag attttcatgt
     5221 tctaaaatat ggcaatggta cattttcaac ccagtatgtc cttgcttaaa gcgaataatc
     5281 actttttcat aagggcgtaa attaaccaca tcttttaacg ctctgccttc tggcttaaag
     5341 gttttaccgt ttaacgtatg ttgaattact tcaaattgag tgccgtgtaa atggaatgga
     5401 tgatccatat ggctttcatt gaagatttcc cactgttcca cttcattcaa tttggtaaca
     5461 aaatcaatac ggttcatatc aaaggtttga ccgttgatta agaacatacc attcatcatt
     5521 ggtggaatag ggttgtccgt tagtgttgta gtagcgtgat gtgtaccgtg attcatcatt
     5581 ggcatattcg tatggttcat cattttttca ctaaagcgaa tttggcgaac ctgctttggc
     5641 tcatcccagt taggcaagga ccttaagctt tcaggtaact gaacaggttc tgatttcacc
     5701 tgaattgtgg ctaaagtgag atctttaggc tgttcttgaa ccatcatctt acggcgatca
     5761 taataaccgc tttgtaaatt gactgttcct tgcgtttcac ccaccataat cacttcaacg
     5821 cgttcggctg gtgtaagtag cagttcatca acaggcgagc gaggtttttc aagtaaacca
     5881 ccttcggtac ccaccacaat ccatttcacc cctggaatgt ttaaacgaaa ataacgagcg
     5941 ctagtggcat tccaaagacg gattcgttca ttcgttttca cttgaatctg gggctgatat
     6001 tgaccgttga ttaacacaaa ttcaccctca cgaccgttta tccaatctaa catcgtgttt
     6061 gctggaatgg tgccatcggc atttaaacgc agatcggaaa tcacccaatg ttgttcagga
     6121 aggtgtgcaa gcggatcatt tttcgcttta acaacgaaag tgcctgctaa gcctttatag
     6181 acttgttcgg aaacatggtc atgtggatga gggtggtacc aatatgtccc agcactgcct
     6241 tgaggtagcg taaagcgata gacttttttc ccttgcggtt caaccatatc catcgggtta
     6301 ccatccgcct catttggtac atctaagccg tgccaatgta ctgttgaggg ttgtggtagg
     6361 tgattgataa attcaatttc tacggtatct ccttcaaata cttcaatttg tggccctggt
     6421 agttgtccgt tataagccca aaattccgtt tctttattgt ctgcgaggcg aattttaatt
     6481 ggctctgctt ttaaagtggc tttgaacagt ccagcttgag tcgattgatt agcaagtttt
     6541 ggtaatatcc cattaagcga taaacctgaa ggcattgcct cagttggcat taggccagta
     6601 ggtactgaat gcatcatcgc catattgcca tgttgtgcgt ggttcatcat attcgcgagg
     6661 ctataaagcg agctactggc taagctaatt ccaattccat aacgtaaaaa atctcgacgt
     6721 ttcattcttt ccccccccct attggttata ggatttaaac acttcacttt caccgttttt
     6781 cttaatcaaa acaacatcat aaggatcttt gcgtccgccg tatgcttcac cgtccatacc
     6841 tggtgaacca attggcattc ccggtgcaga caagcctatt gcatcgggtt tttctgcgag
     6901 taaacgtttg atatcctcag caggtacatg tccttcaatc acatagccat caatcacggc
     6961 tgtgtggcaa gaagcatgtt catatttaat gttaaagcgc ttatgagcat cataattgcc
     7021 tgtttcgttg actttaacct caaaaccgtt tttctgcata tagctgatcc attcattaca
     7081 acagccacaa gtcgggcttt tccacacttc catagaaagt gtttgtgctt gagcaaaagc
     7141 ggttatgcct aaacttaaaa gcaacaatga gcgggtgaat ttatgtaatt tcataagatg
     7201 tccttatttt ttattgagat tgaatggaca tcattttaaa gcttaaccta gggttaaggt
     7261 caagtaatct gaaaaaattc tatcatttga aaatttttct caaaatccta ctgcttgtag
     7321 tagagatatg ccaacgtacc atccactatt attttttaaa atgtgatcag aattattatt
     7381 tatgatgttc tgaatgacca ccttgagtat tcggcatatt catattaggg ttcatttctt
     7441 gatgcatctt catcatttct tcaggcgtta tttcgcccgt catcatttgg ttgtgcattt
     7501 ctgttatttg tttagcatcc atttgcattg aatgagtcat tgctggtgta gtgggttgat
     7561 agcctgctga aataggatct gccatgccaa agatcatagc aaagtgactc acaaataaga
     7621 aaatcacaag ataaaccgca tgtaatttaa tttttgcact atccacgaga tttttatgaa
     7681 taacttttaa ttccatgagt aaaataataa aaataggaaa ggtagctaat aatagtgatg
     7741 caattgatgc gatatctacc caaccacgcc attcagtaat agtcggtacg ataaaacgct
     7801 tacaaaccga ccgtattgac ttactctatc aacaccgtgt tgatcccaat gtgccgattg
     7861 aagaggtggc tggcgctatt aaagatttaa tgaaacaagg caaagttttg aattggggct
     7921 tgtcggaagc aggcgagaaa accattcgca aggcacacgc tattcagcct ttaaccgccg
     7981 tacaaagcga atactcgttg tttttccgtg gtcgtgaaaa agatgtcatt ctcgtttgtg
     8041 aagaattagg gattgggttt gtgccttggt cgccgctggc aatgggagca ttggctggtt
     8101 atgtggacga gaacagccgt tttgaggctg atccaagcaa agatttgcgt ggtattatcc
     8161 cacgctttac acccgaagcg atgcaacaaa atgtgaagtt aatccagctt gtgcaaaaat
     8221 gggcaaaacg caaactttgt acgcctgcac agctttcttt ggcctggcta atggcacaga
     8281 aagcgtttat cgcgccaatt cctgggacga gcaaagcccg tcatttgttg gataatttag
     8341 gcgcagacga tgtgaaattt accgaagaag aattggcgca attccgccaa gaattagaag
     8401 ccattgaaat tgtcggcttg cgtttgcctg aagcaatttt gaatttttca gaagcgaaat
     8461 agttttcaga tacctgaaaa agtcgtaaat aaaaattctt tcttacagcg gtcgttttca
     8521 taaaatcctt tgaaaaacga tcgcactttt tcttgaatat gtagcaagat tagtggaaga
     8581 tttatcgaaa ttgaaagata ttgggaaaag ggtaaaactg gaatcagaca gatgattagg
     8641 caaaacgcta tagtcaataa gtgtaaaaat ggcataatca actcaatgaa tttat
//
LOCUS       DANLYI010000096         8480 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.096, whole genome shotgun sequence.
ACCESSION   DANLYI010000096 DANLYI010000000
VERSION     DANLYI010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 8480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8480
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.096"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            130..1320
                     /gene="malE"
                     /locus_tag="PXD81_001543"
     CDS             130..1320
                     /gene="malE"
                     /locus_tag="PXD81_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005537801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HDL5537558.1"
                     /translation="MKNKCVKFTLATLATLVLSGTVMAKMTEGKLVIWINGDKGYNGL
                     AEVGKKFEKDTGVQVLVEHPDRLEEKFAQVASTGDGPDIMFWAHDRFGGYAQSGLLAE
                     VNVSKEFKDKFVDFAWDAETYNGKIIGYPVAIEAISLIYNKDLVKEAPKSWEEIIELD
                     KKLKKEGKNAIMWNLSEPYFTWPIAASNGAYAFKFANGKYDPKDIGVNNEGAVKALQF
                     VVDMVKNKHISADMDYAVAEASFNKGQSALTINGPWSWGNIDKSGIKYGVTLLPTLNG
                     QASKPFVGVLSAGVNSSSPNKDLAKEFLENYLLTDDGLDAVNKDKPLGAVALKSYQEK
                     LAADPRIAATMANAKNGEIMPNIPQMSSFWYAEKSAINNAVTGRQSVKEALDDAHSRI
                     QKQQ"
     gene            1439..2977
                     /gene="malF"
                     /locus_tag="PXD81_001544"
     CDS             1439..2977
                     /gene="malF"
                     /locus_tag="PXD81_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HDL5537559.1"
                     /translation="MLTQTQSKSTHWLKYLLAGLVLLLDFYLVVLMYSQGEYLFAILT
                     LIILTSGVYIFTNKNTYAWRYVYPGITGMAIFILFPLVATIAIAFTNYSGSNQLSFER
                     AVSVLTEQRYFAGDKYQFTLYPQADNKYQIALTNPTTEQTFVSEPISLATGTNVVVTE
                     QNRPTGEVAPLKVITQNRAALQSMKVVLPNNNELTMSSLRQFSEQKPRYQFDEENKIL
                     LNNETGKRYQANDETGFFQSIDENGHWQSETLEPGYTVTSGFNNFIKIFTDEGIQKPF
                     VQIFIWTVIFSLLTVVFTVILGMVLACLVQWEALKGKAVYRVLLILPYAVPSFISILI
                     FKGLFNQSFGEINMILNQLFGISPEWFNDPLLAKVMILIVNTWLGYPYMMILCMGLLK
                     AIPADLYEASAMDGASTWQNFSKITFPLLLKPLTPLMIASFAFNFNNFVLIQLLTNGR
                     PDMIGTTTPAGYTDLLVSYTYRIAFEGSGTQDFGLAAAIATIIFLLVGGLALLNIKAT
                     KMEL"
     gene            3030..3920
                     /gene="malG"
                     /locus_tag="PXD81_001545"
     CDS             3030..3920
                     /gene="malG"
                     /locus_tag="PXD81_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HDL5537560.1"
                     /translation="MAIVQSKSVRYRVWATHFILICFLALIIFPLLMVIGISLRPGNL
                     AIGDIIPSQISWEHWQAALGFDVTHADGTVTPPPFPVLRWLWNSIKVATITSIGIVTL
                     STTCAYAFARMKFKGKKTILQGMLIFQMFPAVLSLVALYALFDRLGQYVPFLGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDGSLEEAASLDGATPWQAFRLILLPLSVPILAVV
                     FILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLYPQNYLWGDFAAAAVLSAIPITL
                     VFLLAQRWLIGGLTAGGVKG"
     gene            3995..6052
                     /locus_tag="PXD81_001546"
     CDS             3995..6052
                     /locus_tag="PXD81_001546"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HDL5537561.1"
                     /translation="MKNALPYLCLFYTSSLFAQSWQHSHFSDFNDDLEKHFFQSQVQL
                     EKGSYPLRFKFGEQCYQPQQAIKLNQSVALVPCINEAPQLRLFRQGNYLAQIDLRSGT
                     PTPTISVEQQLQHNEAMFQSCPNWDKKPIEIDVSSTFAEGESVSDFYSGNTAIVKNGK
                     VTLMPNENASGLILLEKSNTENTATFDWKNATVYFVLTDRFYNGDPTNDHSYHRQKDG
                     MQEIGTFHGGDLKGLTEKLDYLQQLGVNVLWISSPLEQMHGWVGGGDKGDFPHYGYHG
                     YYHLDWTKLDANMGTEDDLSRFVQQAHQRGIRVLFDVVMNHTGYATLADMQEFNFGGF
                     YLTPDEISRTLGEKWTHWKPKSGQNWHSFNDFIRFNDNQAWQNWWGKEWVRADIADYD
                     SPKFDDLKMSLAALPDLKTESEQAVKLPEFFANKNTNAKTLPNAKVRDYLIGWLSDWV
                     RKYGIDGFRVDTAKHVEKSTWLVLKQSAQQALNEWQKANPNAGFNDRFWMTGEAWGHG
                     VFKSDYYQNGFDAMINFDFQDQAKQGLNCFAHIEPIYAEMNRKLSDFNVLSYLSSHDT
                     RLFFHSDSEQNIEKHKTAANLLLLSPGAVQIYYGDESGRTFGATGSDLIQGTRSDMNW
                     QELQQDPQKQALHQHWQKLTQFRQRHPAIGAGVHQLIKNDSYFAFKRVLNEDKVMVVW
                     AGN"
     gene            complement(6166..>8480)
                     /gene="malT"
                     /locus_tag="PXD81_001547"
     CDS             complement(6166..>8480)
                     /gene="malT"
                     /locus_tag="PXD81_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
                     /protein_id="HDL5537562.1"
                     /translation="LKYWIKHQPSNMTLILISRSIPPLSIASLRVQEQLLEIDINQLM
                     FDHQESTAFFQARLGSELNQQDVIALCNEVEGWPTALQLISLFAKNKSHTLQVPLQDI
                     AKRLAKSNHFHINEYLADEVLNKVDEETRLFILRCSVLHSMNESLVQAVTGEQNSRTK
                     LESLEKQGLFLQQMANSKWQSSDDSWWKFHPLFASFLNLCCQNELYAELPQLHQKAAN
                     AWLQLGYVTEALHHAMQLSDTGLLLNILSKHAWTVFHQGELKLLEESLNCLDYAHLTE
                     HTNLVLLKAWLVQSQHRHIEVGGILADFSRALTENKIELSKTANAEFNVLRAQVAINS
                     GDENTALQLASEALNDLSENAYYAHIVATSIIGEAHHCHGNLAEALAMLQKAERMARQ
                     HHTYHNILWSQLQQSEILLAQGFSQAAYEMLDKASEFVKENHLQKVPMYEFLLRSKGK
                     ILWEWYNLDKAESMAVAGMNALSKFEDKLQCLTLLTKISLARGNLDNTARLLGEVEQL
                     EHSHSYHHDWIANADQVRIFYWQMTNDVSAVRNWLIQNPSPVSDKNHFTQVQWRNIAR
                     SRILLEEYHEAKAILDNLIETAEKFSLISDLNRALIVRNRLYFLQGEKELAQQDLIQA
                     LKLTRQTNFISAFVIEGDVMAQQIRHLLQLNVLDELVLHKAQFILRNINQFYRQKFAH
                     FDENFVNQLLKNPKVPELLKISPLTQREWQVLGLIYSGYSNEQISDELQVAATTIKTH
                     IRNLYQKIGVTNRNEAISYTKELLMLMGYH"
BASE COUNT         2514 a         1718 c         1700 g         2548 t
ORIGIN      
        1 atacttttag cttggggggc gtagagcaaa gggtgatctc aaatagattg tatagcccat
       61 aatttgccat aatttaataa atgggttgtt tcatatccgt ttcttttatt taacttcaag
      121 gagtttttta tgaaaaacaa atgtgtcaaa ttcaccttag ctactcttgc taccttagtt
      181 ctttcaggaa cagtaatggc aaaaatgacg gaaggtaaac tcgttatctg gattaacggt
      241 gataaagggt acaatgggct tgccgaagtc ggtaagaaat ttgaaaaaga taccggtgtt
      301 caagttttag ttgaacaccc ggatagactt gaagaaaaat ttgcacaggt tgcctcaact
      361 ggtgacggtc cggatattat gttctgggct cacgaccgct ttggtggtta tgcacaatcc
      421 ggtttattgg ctgaagtgaa tgtaagcaaa gaatttaaag ataaatttgt cgattttgct
      481 tgggatgctg aaacttacaa cggcaaaatt atcggttatc cggttgcgat tgaagcgatt
      541 tcattaattt ataataaaga tttagtgaaa gaagcaccaa aatcttggga agaaattatt
      601 gagttagata aaaaactcaa aaaagagggc aaaaatgcca ttatgtggaa cttatccgag
      661 ccctatttca cgtggccgat tgcggcttca aacggagctt atgcattcaa atttgctaac
      721 ggcaaatacg atccaaaaga tattggtgtg aacaatgaag gggcagtaaa agcactacaa
      781 tttgtagtcg atatggtgaa aaataaacat attagtgcgg atatggacta tgccgttgcc
      841 gaagcctcct ttaataaagg acaaagtgcc ttaaccatca atggtccttg gtcttggggc
      901 aatattgata aaagcggtat taaatatggt gtaactttac tcccaacatt aaacggtcaa
      961 gcttctaaac cattcgtagg ggtgttaagt gccggtgtaa acagctcaag cccaaataaa
     1021 gatttagcca aagaattctt agagaattat ttactcaccg acgacggttt agatgcagtc
     1081 aataaagata aacctttagg tgcggtggcg ttaaaatctt accaagagaa attagcggct
     1141 gatcctcgta ttgctgcaac gatggcaaat gcgaaaaacg gtgaaattat gccaaatatt
     1201 ccgcaaatgt cttctttctg gtatgccgaa aaatctgcta tcaataatgc agtgaccggt
     1261 cgtcaatcgg tgaaagaagc attagatgat gcacattcac gcattcaaaa acaacaataa
     1321 ttcttcatca ttctgagtgg gttattatga cccactctat ttttagattc accctaaata
     1381 caataaaagt acagtaaaag taccgtaaca aaatgcagta aaaattcgag gtgctgttat
     1441 gctaactcaa actcaatcca aatccactca ttggctcaaa tatctcttag ccggattagt
     1501 cttgctcctt gacttctatc ttgttgtctt aatgtactca caaggtgaat atctttttgc
     1561 tattttaacg ctgattattc taacttccgg tgtctatatt tttaccaata aaaataccta
     1621 tgcttggcgg tatgtttacc ctggcattac cggaatggca atttttattt tgtttccgct
     1681 ggttgcgact attgcaattg cgtttaccaa ctatagtggt tctaatcaat tatcctttga
     1741 acgagcggta tccgtcttaa ctgaacaacg ctattttgcc ggagataaat atcaattcac
     1801 cctttatccg caagcagata ataaatatca aattgcatta accaatccga cgacagagca
     1861 aacttttgtg tcagagccga tttcattggc taccggaacg aatgtggttg taaccgagca
     1921 aaacagacca actggcgaag tcgctcccct taaagtgatt acgcaaaatc gggccgcact
     1981 tcaatcaatg aaagtggttt taccgaataa taatgaactg acaatgagct cattacgtca
     2041 attctccgag caaaaaccac gctatcaatt tgatgaagaa aataaaatat tactcaataa
     2101 tgagactggc aaacgttatc aagcaaatga cgaaaccggt ttcttccaat cgattgatga
     2161 aaacggtcat tggcaaagcg aaacacttga accaggttat actgtcacat caggctttaa
     2221 taacttcatt aaaattttta ccgatgaagg tattcaaaaa ccttttgtcc aaatatttat
     2281 ttggacggtg atcttctctt tattaaccgt tgtctttacc gttattctcg gtatggttct
     2341 tgcctgtttg gtacaatggg aggcattaaa aggtaaagca gtctatcgtg tcttattgat
     2401 tttaccttat gccgtaccat catttatttc gattctgatt tttaaaggac tatttaacca
     2461 aagttttggt gagatcaata tgattttaaa ccaactattc ggcatcagcc ctgaatggtt
     2521 taacgatccg ctccttgcca aagtgatgat cttaattgtc aatacgtggt taggttatcc
     2581 ttatatgatg attttatgta tggggttact caaagctatt ccggctgatt tatatgaagc
     2641 ctccgcaatg gacggtgctt ccacttggca aaacttcagt aaaatcactt tcccattact
     2701 gttaaaacca cttactccgt taatgattgc ttcgtttgca tttaacttta ataactttgt
     2761 attaattcaa ctattaacaa acggtcgccc ggatatgatt ggaacaacca ctccagccgg
     2821 ttataccgat ttattagtca gctacaccta tcgtattgcc tttgaaggaa gtggtacgca
     2881 agatttcggc ttagcagccg ccatcgcaac cattattttc ttattagtcg gtggcttggc
     2941 actcttaaat atcaaagcaa ccaaaatgga attataaaag cgggagggat ttaacaatcc
     3001 cgaccccaaa ctaactttag gagttatata tggcaattgt tcaatctaaa tctgtgcgtt
     3061 atcgggtatg ggcaacccat ttcatcttaa tctgtttcct tgccttaatt attttccctt
     3121 tattaatggt gattggcatt tcgttacgac cgggaaatct ggctatcggt gatattattc
     3181 caagccaaat ttcgtgggaa cattggcaag ccgctcttgg ttttgatgtt acacacgccg
     3241 atggtacagt cacaccgccg ccattcccgg tgctacgctg gttatggaac tccatcaaag
     3301 tggcaacaat tacctctatc ggcattgtaa ccttatctac gacctgtgct tatgcatttg
     3361 cccgaatgaa atttaaaggc aaaaaaacca ttctacaagg tatgctgatt ttccaaatgt
     3421 tcccggcagt gctttcctta gttgccttat atgcgttgtt tgatcgtctt gggcagtatg
     3481 taccgtttct tggtttaaac acacacggtg gtgttatttt tgcctactta ggcggaattg
     3541 ccttacacgt ttggacaatc aaaggctact ttgaaacgat tgatggttca ttggaagaag
     3601 ccgcttccct tgatggtgca accccgtggc aagcgttccg attaatctta ttaccgcttt
     3661 cagtgccgat tttggcggta gttttcattc tttcatttat tgccgccatt accgaagtac
     3721 cggttgcctc actcctattg cgtgatgtaa acagctacac cctagccgtt ggtatgcaac
     3781 aatacctcta tccgcaaaac tatttatggg gtgacttcgc agcagcggcg gtcttatccg
     3841 caattccgat tacgctggtc ttcttattgg cacaacgctg gttaatcggt ggtttaactg
     3901 ccggcggcgt gaaaggctag tttgcaaaaa attctctaaa aacgaccgct tgtcgagctg
     3961 atactgcggt cgtcaatctt tattagggct gtctatgaaa aatgcgttac cttatctctg
     4021 cttattctat acctcaagcc tttttgccca atcttggcaa cattcccatt tttctgattt
     4081 taacgatgat ctcgaaaaac atttttttca atcccaagtt caattggaaa aagggagcta
     4141 tccgcttagg ttcaaatttg gcgaacaatg ttatcaaccc caacaagcga taaaattaaa
     4201 tcaatcggtt gcgttagtcc cttgtataaa tgaagctcca caactgcggt tgtttcgtca
     4261 aggcaactat ctggcacaaa ttgatctgcg aagcggcact ccgacaccca caatcagcgt
     4321 agaacagcag ctacagcaca atgaagcgat gtttcaaagt tgcccgaatt gggataaaaa
     4381 accgattgaa attgatgttt cttccacttt tgccgaaggc gaatcggtta gcgattttta
     4441 ttcaggcaac accgctatcg ttaaaaacgg caaagtcacc ttaatgccca atgaaaacgc
     4501 aagcggtctg attttgctgg aaaaaagcaa taccgaaaat acggcaacgt ttgattggaa
     4561 aaatgccacc gtctattttg tgctgaccga ccgcttttat aacggcgatc cgaccaatga
     4621 ccacagctac caccgccaaa aagacggaat gcaagaaatc ggcacctttc acggtggtga
     4681 tttaaaagga ttaaccgaaa agctcgatta cctacaacaa cttggggtaa acgtattgtg
     4741 gattagctct ccgttagaac aaatgcacgg ctgggttggc ggcggtgata agggggattt
     4801 tccgcattat ggctatcacg gttactatca tttagattgg acgaaattag atgccaatat
     4861 gggtactgaa gacgatttaa gtcgctttgt tcaacaagcc caccagcgtg gtattcgggt
     4921 gctatttgat gtggtgatga accataccgg ctacgcgacg cttgccgata tgcaggagtt
     4981 taactttggc ggattttact taacaccgga cgaaattagc cgcacattag gcgaaaaatg
     5041 gacacactgg aagccgaaat caggacaaaa ttggcacagt ttcaatgatt tcattcgctt
     5101 taacgataat caggcttggc aaaattggtg gggaaaagaa tgggttagag ctgatattgc
     5161 cgattacgac agccctaaat ttgatgattt aaaaatgtcg ctcgctgccc ttcctgatct
     5221 aaaaaccgag agcgaacaag cggtcaaatt acctgaattt tttgcaaata aaaataccaa
     5281 tgccaaaaca ttaccaaacg ccaaagtgcg tgattattta attggttggt tatccgattg
     5341 ggtcagaaaa tatggcattg acggttttcg ggttgatacc gccaaacacg ttgaaaaatc
     5401 cacgtggctg gtattaaaac aatcggcaca acaagcctta aacgaatggc aaaaagccaa
     5461 tccgaacgcc ggttttaacg atcgcttttg gatgacagga gaagcgtggg gacacggcgt
     5521 gttcaaaagt gactattatc aaaacggttt tgatgcaatg atcaatttcg attttcaaga
     5581 tcaggctaaa caagggttaa attgctttgc ccatattgag ccgatttatg ccgaaatgaa
     5641 tcgcaaatta agcgacttca atgtgttgag ctatttatct tcacacgata ctcgattatt
     5701 tttccattcg gatagtgaac aaaatatcga aaaacacaaa accgccgcaa atttgctgtt
     5761 gctttcaccc ggtgcagtgc aaatctatta tggtgatgaa agtgggcgga catttggtgc
     5821 gaccggttca gacctgatac aaggcacccg gtcggatatg aattggcaag agttgcagca
     5881 agatcctcag aaacaggcat tgcatcaaca ttggcaaaaa ttgacacaat tccgccaacg
     5941 ccatccggct attggcgcag gtgtccatca actcatcaaa aatgatagct actttgcatt
     6001 taagcgtgtg ttgaatgaag ataaagtgat ggtggtttgg gccggaaatt aaatctactt
     6061 tagcttcaag ttttctctct tctggggaaa acttgaagcc ggttcgggga aacaagttgc
     6121 aaaaaattac taaaaataga ccgcttgttc cccatgagct tgtgatcaat gataccccat
     6181 caacatcagc agttctttcg tatagctgat tgcttcgtta cgattcgtca caccgatttt
     6241 ctgataaaga tttcggatat gggttttaat cgtggttgca gccacttgca attcatctga
     6301 aatttgttca ttgctatatc cggagtaaat caaacccaat acttgccatt cccgctgagt
     6361 gagcggacta atttttaaga gttccggcac tttcggattt ttcaataatt gattcacaaa
     6421 gttttcatca aaatgggcaa atttttggcg ataaaattga ttgatgttgc gtaaaataaa
     6481 ctgtgctttg tgcaatacca gttcatctag cacattaagt tgcaataaat ggcggatctg
     6541 ctgtgccatt acatcccctt caatcacaaa cgcactgata aaattcgttt ggcgggtgag
     6601 ttttagtgcc tgaattaaat cttgctgagc caattctttc tcgccttgta aaaaataaag
     6661 ccggttacga acaattaatg cgcgatttaa atcgctaatc agagaaaatt tttcagccgt
     6721 ttcaattaaa ttatctaaaa tggctttcgc ctcgtgatat tcctcaagca agattcgaga
     6781 ccgtgcaata tttcgccatt gcacttgggt aaaatgattt ttatctgaaa ccggtgaggg
     6841 gttttgaatt aaccagttcc tcaccgcaga aacgtcattg gtcatctgcc aataaaatat
     6901 acgtacctga tcggcgttgg caatccaatc gtggtgataa gaatgagaat gttctaattg
     6961 ttcgacctcg cctaataatc gggcagtgtt atccaaattg cctcgagcca acgagatctt
     7021 cgtgagtaaa gttaagcatt gtaacttatc ttcaaattta gataaggcat tcattccggc
     7081 aactgccatt gattcagctt tatctaagtt ataccattcc cataaaatct tgcctttaga
     7141 acgtagcaaa aattcataca tcggcacttt ttgtagatgg ttttctttca caaactcgct
     7201 tgctttatct aacatttcat aggctgcttg tgaaaaccct tgggcaagca aaatttcaga
     7261 ttgttgaagc tgtgaccaca aaatattgtg gtaagtatga tgctgacgtg ccatacgctc
     7321 tgctttttgc aacattgcta atgcttctgc cagattgccg tggcaatgat gagcttcgcc
     7381 aataatagac gttgcaacaa tatgagcata ataggcattt tctgaaagat cgtttaacgc
     7441 ttcagacgct aattgaagtg cggtattctc atcaccggaa ttgatagcaa cttgtgcccg
     7501 tagcacatta aattccgcat tggcagtttt gctcaattca attttatttt cagttaatgc
     7561 acgagaaaaa tccgctaaaa taccacctac ttcaatatgg cggtgctggc tttgtaccaa
     7621 ccaggctttg agtaacacca aattagtatg ttcggttaaa tgggcgtaat ctaagcaatt
     7681 taagctctct tccaataatt tgagttctcc ttgatgaaac accgtccaag cgtgtttgct
     7741 caaaatattg agtagtaatc cggtatccga taactgcatt gcgtgatgta aggcttctgt
     7801 aacatagcct aattgtaacc acgcattcgc cgccttttga tgcagttgcg gtaattcagc
     7861 atataactca ttttggcaac ataaatttaa aaaggaagca aaaagcggat ggaatttcca
     7921 ccagctatca tcactactct gccacttact gtttgccatt tgttgtaaaa acaaaccttg
     7981 cttttcgaga gactctaatt tggtgcgact gttttgctcc cctgtaacag cctgcactaa
     8041 agactcattc attgaatgca aaaccgaaca acgcaaaata aacaaacgag tctcttcatc
     8101 gactttatta agcacttcat cggctaaata ttcgttaata tgaaaatggt ttgatttggc
     8161 taaacgtttg gcaatatctt gcagtggtac ttgtagcgtg tgtgatttat tttttgcaaa
     8221 aaggctaatt agctgaaggg ctgtgggcca cccttcaact tcgttacaca gtgcgataac
     8281 atcttgctga tttaactccg aacctaatct tgcttgaaaa aaagcggtac tttcttggtg
     8341 atcaaacatt aactggttaa tatcaatttc caataattgt tcttgcactc ttaaactggc
     8401 aatacttaac gggggaattg atcgagaaat caagataagc gtcatattag acggttgatg
     8461 tttaatccaa tattttaatg
//
LOCUS       DANLYI010000097         8349 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.097, whole genome shotgun sequence.
ACCESSION   DANLYI010000097 DANLYI010000000
VERSION     DANLYI010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 8349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8349
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.097"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            complement(169..807)
                     /locus_tag="PXD81_001548"
     CDS             complement(169..807)
                     /locus_tag="PXD81_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HDL5537563.1"
                     /translation="MELKHTIEQRKTIKAFNSTIKISRTELEEMVALSQLAPSKANLQ
                     PWRFVIIDVEILKAALSKNVAFNGPPCETASAIVVILADLQYEKLLGAIVDQSVETGC
                     LHKEFRERKFDFLLNLHNQATQAEIRDQVLIDTSLAAMQLMLIAKDKGYDTHAIGVFE
                     RPAVLKLLNIDAERYLPVMLLAIGKAANPALPSCRLPLEYTVSWNDGQGFKI"
     gene            complement(949..1743)
                     /locus_tag="PXD81_001549"
     CDS             complement(949..1743)
                     /locus_tag="PXD81_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL5537564.1"
                     /translation="MALLEVNHLNKRFVERSSLFHKQDFYAVKEVSFTLNRKETLAII
                     GENGAGKSTLAKMVAGMVGPTSGELIFRGTPLSFGDYKYRAKHIRMMFQDPNDAFDPN
                     YNIGQILDAPLRLATDLNEEERNERIFNTLKLVGMYPEQALVPISEASSGQKQRIAFA
                     RALILEPEIIIADDTINSLDFSVKTQLINLMLSVQQRQGIAYIYVGQNLGLIKHIADK
                     IMVMQSGEVVEYGLTKEVLLNPQHQITKRLIESHFGKHLSSYAWAS"
     gene            complement(1844..2920)
                     /gene="fbaA"
                     /locus_tag="PXD81_001550"
     CDS             complement(1844..2920)
                     /gene="fbaA"
                     /locus_tag="PXD81_001550"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014990940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HDL5537565.1"
                     /translation="MSLLNIVKPGVVTGDDVQKVFAYAKEHNFAIPAVNCVGSDSVNA
                     VLETAARVKSPVIVQFSNGGAQFYAGKGIKPASGARADVLGAIAGAKHVHQLAEEYGV
                     PVILHTDHAAKNLLPWIDGLLEAGEKHFAETGRPLFSSHMIDLSEEPIEENMEICREY
                     LARMDKIGMTLEIEIGVTGGEEDGVDNSDVDESKLYTQPSEVLYVYDQLSPISPRFTI
                     AAAFGNVHGVYKPGNVKLKPSILGASQEYVSKERGLPAKSLDFVFHGGSGSSREEIRE
                     AISYGAIKMNIDTDTQWAAWDGILQFYKANEAYLQGQLGNPTGPDAPNKKYYDPRVWL
                     RKMEESMSKRLEQSFEDLNCVNVL"
     gene            complement(3017..4192)
                     /locus_tag="PXD81_001551"
     CDS             complement(3017..4192)
                     /locus_tag="PXD81_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012072290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HDL5537566.1"
                     /translation="MSVIKMTDLDLAGKRLFIRADLNVPVKDGKVTSDARIVATIPTL
                     KLALQKGAKVMVTSHLGRPTEGVFEEENSLQPVVDYLNASDLGVPVRLVRDYLDGVEV
                     NENEIVVLENVRINKGEKKNDPELAKKYASLCDVFVMDAFGTAHRAEGSTYGVAEYAP
                     VACAGPLLAAELDALGKALKEPQRPMLAIVGGSKVSTKLTVLDSLSKIADQLIVGGGI
                     ANTFIAAEGHAVGKSLYEEDLIPEAKRLAQVTNIPVPVDVRVGTEFSETAPATEKAVS
                     EVRADESIFDIGEKSSEQLAEIIRNSKTILWNGPVGVFEFPNFRKGTEVISNAIAEAT
                     ANGAFSIAGGGDTLAAIDLFGIKDKISYISTGGGAFLEFVEGKVLPAVEILEKRANG"
     gene            complement(4447..5805)
                     /locus_tag="PXD81_001552"
     CDS             complement(4447..5805)
                     /locus_tag="PXD81_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005603424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HDL5537567.1"
                     /translation="MANQQQERQSWSNRLAYIMTVAGATVGFGATWRFPYLVGEHGGG
                     AYVFLFCIAMIVIGIPMILVENVIGRRMRVNAIDAFAGFANGKKIASGWKILGYMGLL
                     GAFGILAYYMVLGGWVLNYIGSLLTGTLDLSQPVTVGTTQQFFQQSIFNSPMTIIAYT
                     ALFVLVNYFILVKGIVDGIERSVKYLMPILFIFLIVMVVRNITLPGAAEGIAFYLMPD
                     FSKITPKLFVFVLGQVFFALSLGFGVLITLSSYLDKSENLVKTATITGVMNTLIAVLA
                     GFMIFPSLFSFGVAPNSGPTLVFQSLPIVFSNMWGGTVFAVIFFSLLVVAALTTSLTI
                     YEVIITALQEKAKISRQKAIFITLATIFLLGNIPSALSDNILQDVQVMGKSIFDAFDY
                     ISGNILFILTALGSAIFVGFVLKEEAKEELGAGKMFTTIWFNYVRFVIPVIILVIFFN
                     SL"
     gene            complement(5875..6702)
                     /locus_tag="PXD81_001553"
     CDS             complement(5875..6702)
                     /locus_tag="PXD81_001553"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dam family site-specific
                     DNA-(adenine-N6)-methyltransferase"
                     /protein_id="HDL5537568.1"
                     /translation="MNHPKHRAFLKWAGGKYRLIPDIQTHLPKKACLVEPFVGAGSVF
                     LNTDFERYILADINPDLINLFNVVKADVEEYIAQTKQLFLHPQANTEAFYKARRAEFN
                     RSKDIFRRSVIFLYLNRFGYNGLCRYNQKKGYNVPFGRYKTHYFPEKELRFFAEKAQK
                     ATFVCADFEQVFELAKNQLTDYVIYCDPPYAPLAQETNFTQYAGGGFSLEQQGRLATL
                     ALEMKGLGVPVLISNHDTDFTRRIYQSADITQIQVQRSIGQKVGSRVKVGELFALFI"
     gene            complement(6716..7804)
                     /gene="aroB"
                     /locus_tag="PXD81_001554"
     CDS             complement(6716..7804)
                     /gene="aroB"
                     /locus_tag="PXD81_001554"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005611318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HDL5537569.1"
                     /translation="MLQVNVELKERRYPIIIGAGLLNQPASYSPLKSGDKVMIVSNPT
                     VATHYLSVVTNALKELGCHVDSVLIPDGEEYKTLESLNLIFTALLEKNHNRDTTLIAL
                     GGGVIGDVAGYAAASYQRGIRFIQIPTTLLAQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     VSVIVDTNTLHTLSKREVSAGLAEVIKYGAIFDVTFFEWLEKHIDDLVSLKQDELEYC
                     IQRCCQLKADVVARDETEKGDRALLNLGHTFGHAIEARMGYGVWLHGEAVSVGMLEAA
                     ELSRILGDLSPQEVARLEKLLARANLPTISPDGMEPAEYLPYMWRDKKVLAGQLRLIL
                     LKSLGEAYVSSQATESQVLASIERFTQR"
     gene            complement(7828..8349)
                     /gene="aroK"
                     /locus_tag="PXD81_001555"
     CDS             complement(7828..8349)
                     /gene="aroK"
                     /locus_tag="PXD81_001555"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005710312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HDL5537570.1"
                     /translation="MAEKRNIFLIGPMGAGKSTIGRQLAQTLGMEFMDSDSVIEERAG
                     ADIDWIFDVEGEEGFRKREERILNELTQNYGIVLSTGGGSILSKDNRNVLSARGIVIY
                     LETTVDKQFERTQRDKKRPLLQTEDPRKTLEELAKIRNPLYEEIADITIHTDEQSAKV
                     VANQIIDMIDNLQ"
BASE COUNT         2606 a         1834 c         1427 g         2482 t
ORIGIN      
        1 ggtaattgta accaacgact tgcgaaaata attttaccta aaatatttgc cctagtattt
       61 aattccgaat gcatatatct cctataaata gcaatacaaa aataaattac aacgctcaaa
      121 ttttaacaat tttttgccaa aaaattaaga tatttaaccg cttgcacctt atattttaaa
      181 accttgtcca tcgttccaag aaaccgtata ttctagaggt aaacggcaac ttggcaacgc
      241 tgggtttgct gctttaccga tagctagtag cataacaggc aaataacgtt ctgcatctat
      301 attcaataat tttaatacag cagggcgctc aaacacgcct atcgcatgag tatcatagcc
      361 cttatctttg gcaattagca ttaattgcat tgccgccaaa ctagtatcaa ttaatacctg
      421 atctctaatt tctgcttgag ttgcttggtt atgaagattt agtaaaaaat caaatttacg
      481 ttcacgaaac tctttatgca aacacccagt ttctaccgat tgatcaacaa ttgcgcctaa
      541 taatttttca tattgcaaat ctgccaatat caccacaata gcagacgctg tttcacaagg
      601 tgggccatta aaggcaacat tttttgataa tgctgcttta agaatttcaa catcaataat
      661 gacaaaacgc caaggttgca gatttgcttt tgagggagca agctgggata aagcgaccat
      721 ttcttctaat tcagttctac taatcttaat tgttgaatta aaagctttga tagttttacg
      781 ctgttcaatc gtatgtttca attccatctc ttttctccta tctctcaaac aattgagata
      841 gtctataatt taaagattag gaatgtaata attgaaaaga gttattctgc atatttattg
      901 aaataggtca aaattatcaa gtaatcatat tagagcagga gtaaagattt agcttgccca
      961 agcataggaa ctaaggtgct ttccaaaatg actttcaatt aaacgtttcg taatttgatg
     1021 ttgcggattc agtaaaacct ctttcgtcaa gccatactct actacttcgc cactttgcat
     1081 caccattatt ttatcggcaa tatgtttgat cagacctaaa ttttgcccca cataaatata
     1141 agcaatccct tgtcgctgct ggacacttaa cattaaatta attaattgtg ttttgactga
     1201 aaaatccagt gaattaatcg tatcatccgc aataataatt tccggttcta aaattaaagc
     1261 acgagcaaac gcaatacgct gcttttgccc actggaagcc tctgaaatag gaacgagcgc
     1321 ttgctcagga tacattccta ccaattttag cgtattaaaa atacgctcat ttctttcctc
     1381 ttcatttaaa tctgtcgcca agcggagcgg agcatctaaa atttgtccta tattataatt
     1441 aggatcaaag gcatcgttag ggtcctgaaa catcatacga atatgcttag ctcggtattt
     1501 ataatcgcca aatgaaagcg gtgttcctct aaaaatcagt tctccagatg ttggcccaac
     1561 cataccagca accattttag ccaaggtaga tttcccagct ccgttttcac caataatcgc
     1621 taaagtttcc ttgcgattta gggtaaaaga aacttctttt accgcataaa aatcttgttt
     1681 atgaaatagg ctactacgtt caacaaaacg cttattcaga tgattaactt caagtaatgc
     1741 cataataaat tggtgcttgg aaataaagtg atagtttacc gcaaaaaata aataaaaaaa
     1801 gaccgcttat acaagcggcc tgatttatgg aattttttgc aaattataaa acgttcacac
     1861 agtttaagtc ttcgaaagat tgctctaagc gtttagacat agactcttcc attttacgta
     1921 accaaacacg tggatcgtag tattttttgt ttggtgcatc agggcctgtc gggttaccta
     1981 attgaccttg taaatatgct tcgtttgctt tgtagaattg aagaataccg tcccacgcag
     2041 cccattgggt gtcagtgtca atgttcattt tgattgcacc gtagctgatt gcttcacgaa
     2101 tctcttcacg tgaagaacca gaaccgccgt ggaaaacgaa gtctaatgac tttgccggta
     2161 aaccacgctc tttagataca tactcttgtg aagcacctaa gattgatggt tttaatttta
     2221 cattacccgg cttgtaaaca ccgtgtacgt taccaaatgc tgccgcaatc gtgaaacgtg
     2281 ggctgattgg gcttaattgg tcgtaaacat ataaaacttc agaaggttgg gtgtaaagtt
     2341 tagattcatc tacatcagaa ttatctacgc catcttcttc accaccggtt acgccgattt
     2401 cgatttcaag ggtcataccg attttgtcca tacgggctaa atattcacgg cagatttcca
     2461 tattttcttc gattggctct tcagataaat cgatcatatg tgaagagaat aacggacgac
     2521 cggtttctgc gaagtgtttt tcgcctgctt caagtaagcc atcgatccaa ggtaataagt
     2581 ttttcgcagc gtggtcggtg tgaagaatca caggcacacc atattcttct gctaattgat
     2641 gaacgtgttt tgcacccgca atcgccccta atacatctgc acgagcgcct gaagccggct
     2701 taatgccttt acccgcatag aactgcgcac caccatttga gaattgcacg attaccggtg
     2761 attttacacg agcggctgtt tctaatacgg cattaactga atctgaaccc acgcagttta
     2821 ctgccggaat agcgaagttg tgttctttag cataagcgaa aactttttgt acgtcgtcgc
     2881 ctgttactac acccggtttt acgatgttta ataatgacat ttttttcttc ctttgtatga
     2941 aatgtgcaaa agcacattgt taaaatatac ggccaaatct tgcaaaattt tcacaaaatt
     3001 tgaccgcttg taactgttag ccgttagctc gtttttctaa gatttccact gccggtaata
     3061 ctttaccttc cacgaattct aagaacgcac cgccaccggt tgaaatgtaa gaaattttgt
     3121 ctttgatacc gaataaatca atcgcagcta aggtatcgcc accaccggca attgagaatg
     3181 caccgtttgc cgttgcttct gcaatcgcat ttgaaatcac ttctgttcct ttacggaagt
     3241 tagggaactc aaacacgccg accggaccat tccaaaggat ggtttttgaa ttacggataa
     3301 tttcagctaa ttgctctgac gatttttcac caatatcgaa aattgattca tcagctcgta
     3361 cttcactcac cgctttttcc gttgccggtg cagtttcaga gaattcagta cctacacgca
     3421 catcaaccgg aacaggaata ttggttactt gtgctaaacg ttttgcttca gggattaaat
     3481 cttcttcgta taatgattta cccacagcgt gaccttctgc ggcaataaag gtattggcaa
     3541 taccaccgcc cacgattaat tggtcggcaa tttttgaaag cgaatcaagc acagttaatt
     3601 tagtagatac tttagaacca cccacaatcg ctaacattgg acgttgtggc tcttttaatg
     3661 ctttgcctaa tgcatctaat tcagcggcta ataacggacc tgcacacgca acaggggcat
     3721 attctgccac accgtaagtt gaaccttccg cacggtgagc tgtaccgaat gcgtccatta
     3781 caaacacatc acaaagtgat gcatattttt ttgctaactc agggtcgttt ttcttctcac
     3841 ctttgttaat acgcacgttt tcaagcacca cgatttcatt ctcgtttact tcaacaccgt
     3901 ctaagtaatc acgcactaaa cgaacaggaa cacctaaatc tgatgcattt aagtaatcaa
     3961 ctacaggttg taaagagttt tcctcttcaa atacgccttc tgtcggacga cctaaatgcg
     4021 aagttaccat tactttagca cctttttgta acgctaattt taaggttgga atggtagcaa
     4081 caatacgggc atctgatgtt actttgccat cttttaccgg tacgttaagg tcagcacgaa
     4141 taaataaacg tttacccgct aaatcaaggt cagtcatttt aataacagac atagtttgtt
     4201 cctctttaga ttaaaattta aaagtgataa cggtcggatt atatcagttt ctaatcattt
     4261 tgggctaacc tagatcaaat aaatgattaa ttttttatca ttttagaatg ctattatcct
     4321 gactgttctt attccccccc aatatgcttg gttaattaag caagaattgc aaattcccca
     4381 caaaatttaa ccgcttgtca ctaaaaataa agctgtttga aaatccattc aaacagctta
     4441 tagaatttag agtgaattaa agaaaatcac caagataatc accggaatca caaagcgaac
     4501 atagttaaac caaatagtgg taaacatttt acccgcacct aattcctcct tcgcctcttc
     4561 ttttagaaca aagccaacga aaatcgcgct acctaaggcg gttaaaataa acagaatatt
     4621 gccactaata taatcgaagg catcaaaaat gctcttgccc ataacctgca catcttgcaa
     4681 tatattgtca cttaatgctg atggaatatt acccaacaga aaaatagtag ccaaagtaat
     4741 aaaaatagct ttttgacggc taatctttgc cttctcttgc aatgcagtga taataacttc
     4801 ataaatagtt aacgaggttg ttaatgccgc caccactaat agactaaaaa agataaccgc
     4861 aaaaactgtt cccccccaca tatttgagaa cacaatcggc aaactttgga atactaaagt
     4921 cggacctgaa tttggagcaa cgccaaagct aaataatgac ggaaaaatca taaaaccggc
     4981 aagtaccgca attaaggtgt tcatcacccc agtaattgtt gctgttttta ctaaattctc
     5041 gctcttatca aggtagcttg aaagggttat caacacacca aaaccaaggc ttaatgcaaa
     5101 aaatacttga cccagcacaa acacaaacaa ttttggtgtg atttttgaaa aatcgggcat
     5161 cagataaaag gcaatgcctt cagcagcccc cggtaaggta atatttcgaa ccaccatcac
     5221 aatcaggaaa ataaacagaa tcggcatcaa atatttcact gaacgctcaa taccgtccac
     5281 aatgcctttt accaaaataa aataattcac caatacaaaa agtgccgtat aagcaataat
     5341 tgtcattggg ctattaaaaa tactttgttg gaagaattgt tgggtcgtgc caacggtaac
     5401 aggttgagac agatctagtg tccccgtgag taagctaccg atataattta atacccaacc
     5461 accaagtacc atatagtagg caagaatacc aaatgctcca agcaagccca tatagccaag
     5521 aattttccaa cctgaggcaa tttttttacc attagcaaaa ccggcaaaag catcaatagc
     5581 attcactctc attcggcgac caattacatt ctccactaat atcataggga taccaattac
     5641 gatcatcgca atacaaaata aaaacacata agcaccgcct ccgtgttccc ccacaagata
     5701 agggaaacgc caagttgcac caaaaccgac tgttgcacca gcaacggtca taatatacgc
     5761 caatcggtta gaccaagatt ggcgttcttg ttgttgattt gccatttaat actccatcgg
     5821 ctaacaatac cgcttcaacc aacaagcggt cagattttta gaaaatatta caaattaaat
     5881 gaagagtgcg aataactcgc caactttaac ccttgagccc accttctgcc ctattgaacg
     5941 ctgtacctga atttgggtaa tatcggcaga ttgataaatc ctacgggtaa aatcagtatc
     6001 gtgatttgaa attaatacgg gaacgcctaa tcctttcatt tctaaagcca gtgttgccaa
     6061 acgcccttgt tgctctaggc taaaaccacc gccagcatat tgcgtaaaat tcgtttcttg
     6121 tgctaaagga gcgtaaggtg ggtcgcagta aatcacataa tccgttaatt gattttttgc
     6181 caactcaaag acctgttcaa aatccgcaca cacaaaagtt gccttttgtg ctttttcagc
     6241 aaaaaaacgt agttcttttt ctggaaaata atgggtttta taacgcccaa agggaacatt
     6301 gtaccctttt ttctggttat aacggcaaag tccgttatag ccaaatcgat ttaaatacaa
     6361 aaaaatcacc gagcgacgaa aaatatcttt tgagcgatta aactccgcac ggcgtgcttt
     6421 ataaaaggct tctgtattcg cttgtgggtg aagaaacagt tgctttgttt gcgcaatata
     6481 ttcctcaaca tctgctttca ccacgttgaa aagattaatc aagtcaggat taatatcagc
     6541 aagaatataa cgttcaaaat ctgtattcag aaagaccgaa cctgcaccaa caaaaggctc
     6601 gactaaacaa gccttttttg gcaaatgggt ttgaatatct ggaattaagc gatatttacc
     6661 gccagcccat tttaaaaagg ctcgatgctt agggtgattc atttcatttt aaacattaac
     6721 gttgggtaaa acgctcaata gaggctaata cttgagattc tgtggcttgc gatgaaacgt
     6781 aagcttcacc aagtgatttc aataaaatta ggcgtaattg acctgccagt acttttttat
     6841 ctcgccacat ataaggtaga tattctgccg gttccattcc atcaggtgaa attgttggca
     6901 aattggctcg ggccaataat ttttccaagc gagcaacctc ttgaggactt aaatccccca
     6961 gaatacgaga taattccgca gcctctagca taccaaccga aacagcctct ccgtgtaacc
     7021 atacaccata gcccatacgt gcttcaatgg cgtgaccaaa ggtatgtcct aagtttagta
     7081 atgcacgatc tcctttttcc gtttcatctc gagcaaccac gtcagctttt agctggcaac
     7141 aacgctgaat acaatattcc agctcatctt gcttgagact gaccaaatca tcaatatgct
     7201 tttcaagcca ttcaaaaaaa gtaacatcaa agattgcacc gtatttgatg acttccgcca
     7261 aaccagcact gacttctctt ttggataagg tatggagcgt attggtatca acaatcacag
     7321 aaacaggctg ataaaaagcc ccaatcatat ttttaccgag tgggtggttt actgccgttt
     7381 tcccgcctac tgaagaatca acttgagcca gtaaagtggt cggaatttgt ataaaacgaa
     7441 taccacgttg gtaagaggca gctgcatagc ctgcaacatc accgattaca ccaccgccta
     7501 aagcaattaa agtggtatcg cgattatgat ttttttctaa taatgcggta aaaatcaaat
     7561 ttaatgattc tagcgtttta tattcttcac cgtcgggaat caataccgaa tcaacgtggc
     7621 aacccagctc ctttaacgca tttgttacca cactaagata atgtgtcgcc accgttggat
     7681 ttgagacaat catcacctta tctccagatt ttaatgggct ataactagca ggctgattta
     7741 ataaccctgc accaattata atcgggtaac gacgctcttt taattccaca ttgacttgta
     7801 acatatctta gctcctttct tacctggcta ctgtaaatta tcaatcatat caataatttg
     7861 atttgccact actttagcgc tctgctcgtc cgtatgaata gtaatatctg caatttcttc
     7921 atacaatggg ttacggattt tcgctaactc ttccaacgtt ttacgggggt cttctgtttg
     7981 caataaaggg cgttttttat ctcgctgagt acgttcaaac tgtttatcca ctgtcgtttc
     8041 taaataaata acgataccac gagccgataa cacattacgg ttatctttag ataaaatcga
     8101 accaccgcca gttgaaagca cgataccata attttgcgtc agttcattta gaatacgttc
     8161 ttcacgctta cggaaaccct cttcaccttc aacatcgaag atccaatcaa tatccgcacc
     8221 tgctcgttct tcgatcacac tgtcggaatc cataaattcc atacctaatg tttgagcaag
     8281 ctgacgacca atcgtacttt tacctgctcc catcggccca atcaaaaaaa tattacgttt
     8341 ttcagccat
//
LOCUS       DANLYI010000098         8322 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.098, whole genome shotgun sequence.
ACCESSION   DANLYI010000098 DANLYI010000000
VERSION     DANLYI010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 8322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8322
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.098"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            19..3177
                     /locus_tag="PXD81_001556"
     CDS             19..3177
                     /locus_tag="PXD81_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HDL5537571.1"
                     /translation="MITINENTIEQSAIATLQSLGWDYTYGKKILAGLEHEWRERTAE
                     VILKPLLAQAIAKFNPNLPACEVENVVAQVCRADSGDLAERNRQAYDWLRNGVKITYQ
                     LYGEQVSDVVQLIDFQRPENNDFRIVNQLDISGKKGKRIPDLIGFVNGLPLVVFELKN
                     PLKENADIGKAFAQLQTYKDEISDLFVFNQALVISDGIVARIGSLTADFDRFTPWRVV
                     DEKNQSKRIVFEDELTALLQGVMTPKNLLDYVQNFVVFERDGKNRLIKKIGAYHQFYG
                     VNEAVDCTLLAATGNRKIGVFWHTQGSGKSLSMLFYAGKVLSQSSLKNPTLVLVTDRN
                     DLDGQLYATFCGGEALLKQTPIQADGRDELRSALASRSAGGVIFTTIQKFGLMEGELA
                     HPVLNERENIIVITDEAHRSQYGFSQKINHKGEYREGYAKHLRSALPNASFIGFTGTP
                     IELDDKDTQEVFGKYVSIYDFEDAVEDGATVPIIYEPRQISLGESGEFSKVMEEAQQL
                     IDDDENSYNFRLREKLHSVDSRLQKMAEDIIAHYDERTKQQDGKAMVVVMSRAICVKL
                     YEKITALRPEWHSNDVLQGSIKIVMTSNASDPAEWQKHNQDKKTLEKRFKDPDDPLNI
                     VIVRDMWLTGFDAPCCNTMYIDKPMSGHNLMQAIARVNRVFRNKSRENGGLIVDYVGL
                     TDELEKAMKQYTNAGGKEKPVRDISAVLEKMVEHITVIRGQFATPIDGQAVDIAKMLQ
                     ISEPPKLLNAILTSANHILALDRIQPPDNTAKDKTPRKNAFLQSVRLAKKGYALCGAL
                     KAVEPYKQELAFYDAVRATIIKNSTAPRNSSSENDRLLQLTALMNRAVQSDGVVDLFD
                     LLKKDRPNINLLSDEFLETVKNSPTKDLWLSAMERYLASQLREQSGANLATKKAFEQK
                     LKEAMNQYHNHNLSVLEILEELIALAKEFEARQKRGEALGLSPAEMAFYDALARNESA
                     VREMGDEVLMKLAKDITDKLRKSVTVDWQYKDSVRAKMRTLIRIALRTYKYPPDLQAE
                     AIEFVLQQAEEIAGEFAITETA"
     gene            complement(3211..6102)
                     /gene="glnE"
                     /locus_tag="PXD81_001557"
     CDS             complement(3211..6102)
                     /gene="glnE"
                     /locus_tag="PXD81_001557"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HDL5537572.1"
                     /translation="MLTTLIPLSQLNLPPNLQNSEQIQPLVTAISLSNFVHQTLQKQP
                     DLLAEWLAKFPTPDDCCNYTERLAKILARVENEEMLGRVLRQFRHRELARLSFIQSNK
                     LATVELVFQHLSDLAESLILAARDWLFQRCCAEYGTPKNTLGETQELLILGMGKLGGR
                     ELNFSSDIDLIFTYPDIGETEGGRKSIENSKFFTRMGQRLIKVLDEITADGFVYRTDM
                     RLRPFGDSGALVLSFTAMEDYYQEQGRDWERYAMIKAKILGEDSQNLNHRYLKQMLRP
                     FVYRRYLDFSAIQSLREMKEKISREVVRRNLTDNIKFGAGGIREVEFIAQTFQMIRGG
                     RDKILQERSLLKVLPRLAELNLLTQTQVETLHNAYIFYRQIENVLQAIDDKQTQTLPT
                     DEASQARLVFACQSYYQQDLYSEKTHWVEHSFNDWQQFMAVLSQYQQAVRQIFNEIIG
                     EEETQANCNPVNEKLAEWKDILHYNIRLEDLSAVLKGYPKVAENDYTEIFRHFSTTFQ
                     DWVKRPIGVRGREVLRNLMPRIADSIFKGEDHLLLLPRVLNIVDKITTRTTYLELMLE
                     KEQILPQLLALCSKSVMIAEQIARYPMLLDELMSHRGLTDVQAFEKYQSALQDYLIRI
                     PEEDEEALIDGLRQFKQTQILRIAAADILGVLLVMKISDHLTYLAEAIINAVVNMAWK
                     QVSQRFGVPEHLEADEKGFAVIGYGKLGGIELGYNSDLDLVFLHNAPEDSQTVGGKKE
                     ISSHQFYLKLAQKINSIFNLNTSAGVLYEVDMRLRPSGEAGLLVSTFNAYEHYQKNEA
                     WTWESQALVRTRCVFGAENLKQAFEKIRQSTLAQPRASGQLRQEICEMRQKMYQHLSS
                     HSAEQFHIKQDQGGITDIEFIAQYLVLAHSHQHPKMAVWSDNVRIFDSAVECGILSSE
                     QSEQLQHCYTALRNKIHHLNLLRKDSVVDASEFTTERAFVREMWQRLLA"
     gene            complement(6274..6555)
                     /locus_tag="PXD81_001558"
     CDS             complement(6274..6555)
                     /locus_tag="PXD81_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006717394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HDL5537573.1"
                     /translation="MYYVIFAQDNPNMLEKRLEVRPAHLARLEKLQAEGRLLIAGPNP
                     TEDGSSVTGSTVIAEFNSLEEAQQWASQDPYVEAGVYSDVIIKPFKRVF"
     gene            complement(6557..7021)
                     /gene="yciA"
                     /locus_tag="PXD81_001559"
     CDS             complement(6557..7021)
                     /gene="yciA"
                     /locus_tag="PXD81_001559"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HDL5537574.1"
                     /translation="MKDKPYGKAPEGKLILRTLAMPSDTNANGDIFGGWIMSQMDLGG
                     AILAKELAKGRVVTVTVDKMIFHRPISVGDVVCCYGKLMHIGRSSMQVKVEVFIKQVY
                     EGNRERYLVTEALFTYVAIDKEGKPRAVPRENNPELDEALSLIDLTPEKQGE"
     gene            complement(7011..7568)
                     /locus_tag="PXD81_001560"
     CDS             complement(7011..7568)
                     /locus_tag="PXD81_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HDL5537575.1"
                     /translation="MKQLLEFIPLILFFVVYKMSGIQMASIALVIATIVQMIALKMLY
                     GKIEKQQIIMGGSVVFFGLLSAYFNEVKYLQWKVTIVYALFALVLLVSQFVFKTPLIK
                     KLLGKEIQLPEAVWNKINFGWAVFFLICMLLNIYISQYLSEEIWVDFKSFGIIGLTLV
                     ATVITGFVLYPYLKDAEKQEQKNER"
     gene            complement(7577..>8322)
                     /locus_tag="PXD81_001561"
     CDS             complement(7577..>8322)
                     /locus_tag="PXD81_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL5537576.1"
                     /translation="IKFVDLLQQSWNFMRNQQAFSLFAIVTIVVVQLVFILVSPNSPE
                     LMAESQPQQLQIDASKMVSILLPTILLGVVNLLINVLMIFNIQSINDGNYRQFFQNAG
                     NALKHFLPVLLLQFVMVLPLSLGASFAMASPETVIIALPVLVVGFYFFIKLSLVIYAY
                     LLEKPQKSLSESIKFTLQLSRGKMLPLILFCVISYLLPGMLSRLFTVFGNDFIGIFIT
                     IVLSAVINVFMAIFSFRFYQVYRQMPAVR"
BASE COUNT         2441 a         1818 c         1792 g         2271 t
ORIGIN      
        1 tggggagatg gagttgtgat gattaccatc aacgaaaaca ccattgaaca atccgccatt
       61 gcaactttgc aaagcttggg ctgggactac acctacggca aaaaaatttt ggcaggttta
      121 gaacacgaat ggcgggagcg aaccgctgag gtgattttaa agccgctttt ggcgcaagcg
      181 attgcaaaat tcaacccgaa tttgcccgct tgtgaggtgg aaaatgtggt ggcacaggtg
      241 tgccgtgccg acagtggcga tttggcggag cgtaatcgtc aggcttatga ttggctaaga
      301 aatggggtca aaatcactta tcagttgtac ggtgagcagg tgtctgatgt ggtgcagctg
      361 attgattttc agcgtccaga aaataacgat tttcgcattg tcaatcagct ggatattagc
      421 ggcaaaaaag gcaaacgcat tccggatttg attggctttg ttaatggctt gccgctggtg
      481 gtgtttgagc tgaaaaatcc gctcaaagaa aatgccgaca ttggcaaggc gtttgcccaa
      541 ctgcaaacct ataaagatga aatttctgat ttgtttgtgt ttaatcaggc actcgtgatt
      601 tcagacggca ttgtcgcccg catcggttcg ctgaccgctg atttcgaccg ttttacccct
      661 tggcgtgtgg ttgatgaaaa aaatcagagc aaacgcattg tgtttgaaga tgaactcacc
      721 gccctgctgc aaggcgtgat gacacccaaa aatctcttgg attatgtgca gaattttgtg
      781 gtgtttgaac gggacggcaa aaaccgctta attaagaaaa tcggagcata tcatcagttt
      841 tatggcgtga atgaagcggt ggattgcacc ttgcttgccg ccacaggcaa ccgcaaaatc
      901 ggcgtgtttt ggcatacgca aggttcgggc aaatcgcttt cgatgctgtt ttatgcaggt
      961 aaagtattaa gccaaagcag cctgaaaaat ccaactttgg tgttggtaac cgaccgcaac
     1021 gatttggacg gtcagcttta cgccactttt tgcggtggcg aggcactgct caaacaaacg
     1081 ccaatccaag ccgatgggcg agacgaactc cgctctgccc ttgccagtcg ttcggcaggc
     1141 ggtgtgattt ttaccaccat tcaaaaattc ggcttaatgg agggtgagct tgcccaccct
     1201 gtattaaacg agcgggaaaa catcattgtg attaccgatg aagcacaccg ctcacaatat
     1261 ggttttagcc aaaaaatcaa ccacaaaggc gagtatcgtg agggttatgc caagcattta
     1321 cgcagtgcat tgccgaatgc ctcttttatt ggctttactg gcacaccgat tgaacttgat
     1381 gacaaagaca cccaagaagt cttcggtaaa tatgtctcga tttatgattt tgaagatgcg
     1441 gtggaagatg gggcaaccgt gccgattatt tatgagccac gccaaatcag cttaggcgaa
     1501 agtggcgagt tttccaaagt gatggaagag gcacaacaac tgattgatga cgatgaaaac
     1561 agctataact tccgcctgcg tgaaaaactg cacagcgtgg atagccgttt gcaaaaaatg
     1621 gcggaagata ttattgccca ttacgatgag cgtaccaaac agcaagacgg caaggcgatg
     1681 gttgtggtga tgagccgtgc catttgcgtg aaattgtacg aaaaaatcac cgcacttcgt
     1741 ccagaatggc actcaaacga tgtgcttcaa ggcagcatta aaattgtgat gacaagtaat
     1801 gccagcgacc ctgccgagtg gcaaaaacac aatcaggata agaaaacctt agaaaaacgc
     1861 tttaaagacc cagacgatcc gctcaacatc gtgattgtgc gggatatgtg gctgacaggc
     1921 tttgatgcgc cctgctgtaa cacaatgtac attgataaac caatgagcgg acacaacctg
     1981 atgcaggcaa tcgctcgggt aaatcgggta tttcgcaaca aaagccgtga aaatggcggc
     2041 ttgattgtgg attatgtggg cttgaccgat gagctggaaa aagcgatgaa gcagtacacc
     2101 aacgcaggcg gtaaagaaaa gccggtgcgg gacatttcag cggtgctgga aaaaatggtg
     2161 gaacatatca cggtcattcg tgggcaattt gccacaccga ttgacggaca agcggttgat
     2221 attgccaaaa tgttgcaaat cagcgaaccg cctaaactgc tcaatgcgat tttgacatca
     2281 gccaaccata ttcttgccct agaccgcatt cagccacctg ataacactgc caaagacaaa
     2341 accccacgca aaaacgcctt tttgcaatcg gtacgcttgg cgaaaaaagg ttatgctttg
     2401 tgtggggcat taaaagcagt tgaaccctat aaacaggaac tggcgtttta tgatgcagtg
     2461 cgtgccacca ttatcaaaaa cagcaccgct cctcgcaatt cttcgagcga aaatgaccgc
     2521 ttgttgcagc ttaccgcctt gatgaatcgt gcggtacagt cggatggtgt ggtggattta
     2581 tttgatttgc tgaaaaaaga ccgcccaaac atcaacctgc tttctgatga gtttttggaa
     2641 acagtaaaaa acagcccaac caaagatttg tggctatcag caatggagcg ttatcttgcc
     2701 tcacaactgc gagagcaaag cggtgccaat cttgccacaa aaaaagcatt tgaacagaaa
     2761 ctcaaagagg cgatgaacca ataccacaac cacaatttaa gcgtattgga aattttagaa
     2821 gagctgatcg ccctcgccaa agagtttgaa gcccgccaaa aacgagggga agcgttggga
     2881 ctaagccctg cggaaatggc gttttatgat gctttggcac gtaatgaaag tgcggtgcgg
     2941 gaaatgggcg atgaggtatt gatgaaactt gccaaagaca ttaccgataa attacgcaaa
     3001 tcggtcaccg tagattggca atataaagac tcggttcgtg ccaaaatgcg aaccttaatc
     3061 cgcattgctc tgcgtaccta taaatacccg cccgatttac aggcggaagc gattgagttt
     3121 gtgttgcaac aagcggaaga gatcgccgga gagttcgcta tcaccgaaac ggcgtaatct
     3181 atattagggc gtgcaatgta cgccctaaat ttatgctaat aacctctgcc acatttcccg
     3241 cacaaatgct cgctcggtgg taaattcact tgcgtccacc acgctgtctt tgcgtaataa
     3301 gtttaggtgg tggattttgt tacgcagtgc ggtgtagcaa tgttgtagct gttcgctttg
     3361 ttcggaactt aaaataccgc attctactgc gctgtcgaaa attcggacat tatccgacca
     3421 tactgccatt ttcgggtgtt ggtggctgtg ggctaatacc aaatattggg cgataaattc
     3481 aatatcggta atacctcctt gatcttgttt aatatgaaat tgctcggctg aatgcgagct
     3541 taaatgttgg tacattttct gtcgcatttc gcaaatttct tggcgtaatt gaccgcttgc
     3601 acgaggctga gcaagcgtgc tttggcggat tttttcaaag gcttgtttga ggttttccgc
     3661 cccaaataca catcttgtcc gcacgagggc ttggctttcc cacgtccacg cttcattttt
     3721 ttgataatgc tcgtaggcat tgaaagtgct gactaaaagc ccagcctcgc ctgatgggcg
     3781 aagtcgcata tccacttcat atagtacgcc ggcactggta ttaaggttaa aaatcgagtt
     3841 aattttttga gccaatttta agtaaaattg atggcttgaa atctcttttt taccgccaac
     3901 agtttggcta tcctctgggg cattatgcaa gaaaaccaag tcgagatctg aattatagcc
     3961 cagttcaatc ccgcctagct tgccgtagcc aataactgca aacccttttt catcagcctc
     4021 taaatgttcc ggcacaccaa agcgttggct gacttgcttc cacgccatat ttaccaccgc
     4081 attaataatc gcttctgcta agtaagtaag atgatcgctg atcttcatta ccagcagcac
     4141 gcctaaaata tcagcagcgg cgatgcgtaa aatttgggtt tgtttgaact gacgtaagcc
     4201 atcaatcagt gcttcttcat cttcttcagg aatgcggatt aaataatcct gcaaggcaga
     4261 ttgatatttt tcaaaagcct gcacgtcggt taagcctcta tggctcatca gctcatcaag
     4321 tagcattgga tatcgagcta tctgttccgc aatcataacc gacttactac acaatgctaa
     4381 aagttgaggc agaatctgct ctttttcgag cattaattcc aaataggtag ttcgggtggt
     4441 gattttatcg acaatattca acacgcgagg caataacaat aaatgatctt cacctttaaa
     4501 aatgctgtcg gcaattctag gcattaaatt gcgtagcacc tctcgccctc ttaccccaat
     4561 cgggcgtttt acccaatctt gaaaggtagt agaaaagtga cggaaaatct ccgtataatc
     4621 attttctgca acctttggat aaccttttag cactgcggat aaatcttcaa ggcgaatgtt
     4681 atagtgcaaa atgtctttcc attcggctaa tttttcatta accggattgc agttggcttg
     4741 tgtttcctct tcaccgataa tttcattaaa aatctggcgt actgcttgtt gatattggct
     4801 caatactgcc ataaattgtt gccaatcgtt aaaggaatgc tccacccaat gcgttttttc
     4861 gctatataga tcttgctgat aatagctttg gcaagcaaat acaaggcggg cttgcgaggc
     4921 ttcatcagtg ggtagggttt gagtttgttt atcgtcaatc gcttgtaata cattttcaat
     4981 ttgacggtaa aaaatatagg cattgtggag cgtttccacc tgcgtttggg ttaataaatt
     5041 taattctgcc aagcgtggta gcacttttaa caggctacgt tcttgtaaaa ttttatctcg
     5101 cccgccacgg atcatttgga aagtttgggc gataaactcc acttcccgaa tcccaccggc
     5161 accaaattta atattatcgg ttaaattacg gcgaaccact tcacggctga ttttttcttt
     5221 catttcacgc aacgattgga ttgcactgaa atccagataa cggcggtaaa caaaagggcg
     5281 gagcatttgc tttaaatagc gatgatttaa attttggcta tcctcgccta aaatttttgc
     5341 tttaatcatc gcataacgtt cccaatcacg cccttgctct tggtaataat cttccattgc
     5401 agtaaaactc agcaccaagg ctccgctatc accaaagggg cgaagtcgca tatcggtacg
     5461 atacacgaaa ccatcagcgg tgatctcatc taatacttta attaaccgct gccccattcg
     5521 ggtaaaaaat ttgctgtttt caatcgattt gcgtccacct tcggtttcac caatatcggg
     5581 gtaggtaaaa atcaggtcaa tatcggaaga aaaattaagc tctcgtccgc ccaatttgcc
     5641 catacctaaa atcagcagtt cttgtgtttc gccaagagtg ttctttggcg tgccatattc
     5701 ggcacagcaa cgctgaaaca accaatctcg agcggctaga atcaggctct cggctaaatc
     5761 cgacaaatgc tgaaatacca attctaccgt cgctaattta tttgactgaa taaagctcag
     5821 acgagccaat tcacggtggc gaaattgccg caaaaccctc cccaacattt cttcattttc
     5881 cactcttgct aatatttttg ctaaacgctc agtataattg cagcaatcat caggcgtagg
     5941 aaacttggca agccattctg ccagtaaatc cggctgtttt tgcaaggttt gatgcacgaa
     6001 attggaaagc gaaatcgccg tcacaagcgg ttgaatttgc tcagaatttt gcaaattggg
     6061 cggtaaattg agttgggata agggaattag ggtggtaagc ataaatttag gctcaaaatt
     6121 gaaaatagat agaattttac gctcgatcaa ttcccctctt tagcaaagag ggcttagggg
     6181 agatttggca gaggtaattt tactctcata cgaaacatta atatcgtaat caaatctccc
     6241 ccagcccctc ttttctaaag aggggagaaa aaattaaaac actcttttaa acggtttaat
     6301 aatcacatcg ctatacacac cggcttccac ataaggatct tggcttgccc attgttgagc
     6361 ttcttcaagt gagttgaact ctgcaattac agttgaaccg gttacgcttg agccgtcttc
     6421 agtcgggtta ggccctgcga ttaataatcg accttctgcc tgtaattttt ccaaacgagc
     6481 caaatgagcc gggcgaactt ctaaacgttt ttccaacata ttgggattat cttgggcaaa
     6541 gataacataa tacatactat tctccttgtt tttcaggggt taaatcaatt agcgataatg
     6601 cttcgtctaa ttcggggtta ttttctctcg gcacagcacg cggtttgcct tctttatcaa
     6661 tcgctacata ggtaaataag gcttcggtta cgagataacg ttcgcgattg ccctcataaa
     6721 cttgtttgat aaagacctcg actttcactt gcatcgaaga acgccctatg tgcattaact
     6781 tgccgtagca gcagaccaca tcaccgactg aaatcgggcg gtggaaaatc attttatcta
     6841 ccgttaccgt aacgactcta ccttttgcca actctttggc taaaattgca ccgcctaaat
     6901 ccatctgcga cataatccaa ccgccgaaaa tatcgccatt ggcgttagta tctgacggca
     6961 ttgccaaagt tcttaaaatc agtttccctt ccggggcttt tccatagggt ttatctttca
     7021 tttttttgtt cctgcttttc agcatctttt aagtaagggt aaaggacgaa accggtaata
     7081 accgttgcga ctaaggttaa gccaataatg ccaaaggatt tgaaatcaac ccaaatctct
     7141 tccgataaat attgactgat ataaatattc agcagcatac aaatcagaaa gaatactgcc
     7201 caaccgaagt ttattttatt ccacactgct tcaggcaact gaatttcttt gcctaataat
     7261 tttttgatta atggtgtttt gaacacaaat tggctcacta atagcaccaa tgcaaacaga
     7321 gcgtaaacaa tggttacttt ccattgtaaa tatttaactt cattaaaata ggcactcagt
     7381 aagccgaaaa agaccaccga gccacccata ataatttgct gtttttcaat tttgccgtag
     7441 agcattttta aggcgatcat ttgcacgatt gtagcgataa ccaatgcgat agacgccatc
     7501 tgaataccgc tcattttata cacgacaaaa aacagaatca gcggaataaa ttcaagtaac
     7561 tgtttcattg atactcctaa cgcactgccg gcatttggcg gtaaacttgg taaaagcgga
     7621 agctgaaaat cgccataaag acgttaatta ccgcacttaa aacaatggta atgaatatgc
     7681 caataaagtc attaccaaac actgtgaaca aacgagataa catccccggt aataaataag
     7741 aaatcacaca aaataaaatc agcggtagca tttttccacg tgataattga agcgtaaatt
     7801 taatactttc cgatagactt ttctgcggtt tctccaataa ataagcataa atcactaacg
     7861 ataatttgat aaaaaaataa aaaccgacta ccaacaccgg caatgcaata atcactgttt
     7921 caggcgatgc cattgcaaaa gatgcaccga gagaaagcgg taacaccatc acaaattgta
     7981 acaacagcac aggtaggaag tgctttaatg cgtttcctgc attttggaaa aattgacgat
     8041 aattgccatc gttaatcgat tggatattga aaatcattaa gacattgatc aaaagattga
     8101 caacacctaa taaaatagtt ggcaatagta tagacaccat tttagaggca tcaatttgaa
     8161 gttgttgcgg ttgcgactcc gccattagct caggcgaatt tggcgacact aaaataaaaa
     8221 ccaactgcac aaccacaatg gtgacaatgg caaacagtga aaatgcttgt tgattacgca
     8281 taaagttcca actttgctgc aataaatcga caaatttaat tg
//
LOCUS       DANLYI010000099         8238 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.099, whole genome shotgun sequence.
ACCESSION   DANLYI010000099 DANLYI010000000
VERSION     DANLYI010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 8238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8238
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.099"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            complement(<1..136)
                     /locus_tag="PXD81_001562"
     CDS             complement(<1..136)
                     /locus_tag="PXD81_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HDL5537577.1"
                     /translation="MQQKSKREKLILIGAGGYAKSVLDSLDHEQYEFCGLIDNFKPEG
                     S"
     gene            269..1012
                     /locus_tag="PXD81_001563"
     CDS             269..1012
                     /locus_tag="PXD81_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005615381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HDL5537578.1"
                     /translation="MFEFIKLITAMILPPFNIILLWLLALILFSLGYKYAAYFFALLG
                     ILILYIFSIPYTSQKLGDSLVEAEPFTIEKYKNAQAIVVLGGGLRDSQELFGKLAITG
                     IPLERMRYAAYLHKETGLPILATGSSPNGTSEAKVMADEFKMFFNIETKWLENKAKTT
                     KENAIFSREILEKENIHKIILVTNQWHMKRAEMLFSQQGFEVMSASVGSGDTPDHYKL
                     NFMHFIPQAGAMNSNMLALKEWIGYWKEK"
     gene            complement(1089..3287)
                     /gene="priA"
                     /locus_tag="PXD81_001564"
     CDS             complement(1089..3287)
                     /gene="priA"
                     /locus_tag="PXD81_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HDL5537579.1"
                     /translation="MALNVPLHRYFDYLLPPAFNACQGGRVVVPFGRQTKIGIVVDFP
                     QTSDVPTEKLKPIKAALDSSPIFDSEMWQLLNWAARYYHAPIGEVLTGALPIKLRNGD
                     STERVQPNYFVVTEQGRKSLIIGENKKAKKQVERLTELAECAKYFEKPTACSPSAWKG
                     LLEKGYIEKIDVPFMAQAWQDSLGEFPLVNTNNRLILNKQQTLVVSRLNAQTTFASFL
                     LNGVTGSGKTEVYLQLIETVLKREEQVLLLVPEIGLTPQTVQRFKARFNVEIDVLHSN
                     MNETERLNAWLRAKNGESAIVIGTRSALFTQFQKLGVIILDEEHDSSFKQQDGWRYHA
                     RDLAVVRAKNANIPIVLGSATPSLESLHNVQNGKFIELTLTARAGNAQLANQQIIDLK
                     TQRVTAGLSERLLSMMKTQLEQGNQVMLFLNRRGFAPVLLCHECGWVCECEACNKPFT
                     YHQKQKVLRCHHCATQRVIPRQCGHCGSTHLITTGVGTEQLEQVLNQQFPHYQVTRID
                     RDSTARKGALENHLTAIREGKSQILIGTQMLAKGHHFPNVTLVAIVNVDSALFSTDFR
                     AEEKLAQLYIQVAGRAGRAEKKGEVVLQTHYPDHPLLKTLLEQGYRAFADEALKMRQL
                     MSLPPFSSQVLFKATGKDNQQTLQLLEQLADYFSQKIQQQNGQGFQLPPPFSAPMAKK
                     AGHYRWLLLIQHPLRGALQQLLDAFDQERESLNIPNNIRLNIDIDPQEIS"
     gene            complement(3299..3911)
                     /locus_tag="PXD81_001565"
                     /pseudo
     CDS             complement(3299..3911)
                     /locus_tag="PXD81_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005818858.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
     gene            complement(3904..4803)
                     /gene="rarD"
                     /locus_tag="PXD81_001566"
     CDS             complement(3904..4803)
                     /gene="rarD"
                     /locus_tag="PXD81_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HDL5537580.1"
                     /translation="MLKGILLSLSASVLFGCMYYLAIFLKPLAGVSIFGIRMLVTIPF
                     LFLALYLFKKQNEFWVFLQRLKQEPYLLLVILITASIVGGQMWLFLWAPNSGKAIEVS
                     MGYLLMPIIMVAFGKVVYKETLSLNKWLAIVVAFIGVASNILFTGKISIASLFVCTGY
                     PIYFYLRRKFGLSHLHSFVVEILYLLPVACYFISQTDMQYVETQNLNIYYFIALLGLI
                     SGTALISYTLASTILPFNLLGLLGYVEPLLMLVVSFLIGERLANESYLLMICLLISIS
                     FLVLDGMFALRQQRKRKLSVNNE"
     gene            complement(5030..6247)
                     /locus_tag="PXD81_001567"
     CDS             complement(5030..6247)
                     /locus_tag="PXD81_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HDL5537581.1"
                     /translation="MDRFPKSDKLAQVRYDIRGPIHKEALRLEEEGHKILKLNIGNPA
                     PFGFEAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIVQYYQSKGMRGMDVNDVYIGN
                     GVSELITMSMQALLNDGDEILIPMPDYPLWTAASTLAGGKAVHYLCDEENEWFPDLED
                     IKSKITPRTKGILVINPNNPTGAVYSRQILLEIAELARQHNLIIFADEIYEKIVYDGA
                     VHHHIAALAPDVLTVTYNGLSKAYRVAGFRQGWMVLSGPKHQAKGFIEGLDMLSSMRL
                     CANTPMQHAIQTALGGYQSINEFVLPGGRLLEQRNKMHELLVQIPGISCVKAKGALYM
                     FPKIDTEMYGIKDDQKFIYDLLRQEKVLLVQGSGFNWKKPDHFRVVTLPYAHQIEEAI
                     GRLANFLKTYRQD"
     gene            6435..7739
                     /locus_tag="PXD81_001568"
     CDS             6435..7739
                     /locus_tag="PXD81_001568"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase"
                     /protein_id="HDL5537582.1"
                     /translation="MLIFHQLKQELTKSYSKVGREQGLVAIQASMPLLEANIPLLGWL
                     KTQKTSYPHFFLQYRDTDQTIATIGTLRQFYTLEAAQSFIQQHHFPLIGGLQFEGNTQ
                     FILPQFSLVKNQQNLTACFYFDSDNFEQQAVIFEQFLANFSQITELGLTDNPLVSMSA
                     TSHFEQWKANIEKAIVSIEQGDFQKVVLANAMRLHFEQPICAYSLLAQSQKTNLGCYH
                     FLWAESADVAFLGSTPERLYQRQGLHFSTEALAGTAAVTTSEVQTELNAQWLLSDPKN
                     IYENQLVVDDIENQLKDCVTEFAVSEAKIKRLHNVQHLRRQIQATLKPDTSDSDCLSK
                     IHPTAAVAGLPRQKAKGFITENEPFTRHWYAGTLGVMGNEEAEFCVTLRSALISQNSI
                     TLYAGAGIVSGSDPQSEWQEIKRKAQGMAKLLDVDLVTKGEL"
     gene            7898..>8238
                     /locus_tag="PXD81_001569"
     CDS             7898..>8238
                     /locus_tag="PXD81_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_046701250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL5537583.1"
                     /translation="MKITHCKLKKSLQKKLLEFFVAEVTARTAADLLGIQPNTAALFY
                     HKIRLVIEHHLALEAHEIFEGKIEVDESYFGGHRKGKRGRGAAGKVAVFGLLKRQGKV
                     FTKYQIRNANAG"
BASE COUNT         2504 a         1777 c         1615 g         2342 t
ORIGIN      
        1 cactaccttc aggcttaaag ttatcgataa gtccacaaaa ttcatattgt tcgtgatcaa
       61 gtgaatctaa cactgatttg gcatagccac cggcaccgat caaaatcaat ttttctcttt
      121 tagacttttg ttgcattgct atcacccatg atgaatttca attcatatgg agtattctaa
      181 tacttttcaa gatagaaaaa tagacaagtc ttatataatt taggaaaatg aacatatctt
      241 tacttatttt tactacgata ttctttctat gttcgagttc attaaactga tcaccgcaat
      301 gatcctcccg ccatttaata ttattttgct gtggttgtta gcattaatcc tcttttcttt
      361 aggctataaa tacgctgctt acttctttgc cttgttaggt attctgattt tatatatctt
      421 cagcattccc tatacttcac agaaattagg ggattcatta gtcgaagcag agccatttac
      481 catagagaaa tataaaaatg ctcaagcgat tgtggtgcta ggtggcgggc tacgagacag
      541 ccaagagtta ttcggcaaac tggcaattac cggtattccg ctggaaagaa tgcgttatgc
      601 cgcttatctg cataaagaaa ccggcttacc gattttagcg accggaagca gcccaaatgg
      661 gacttctgag gcaaaggtta tggcagatga atttaaaatg ttcttcaata ttgagaccaa
      721 atggctggaa aataaggcga aaaccaccaa agaaaatgcg attttcagcc gtgagatttt
      781 agaaaaagag aatattcata aaatcattct tgttaccaac caatggcata tgaaacgggc
      841 tgaaatgctg tttagccaac aaggatttga ggtgatgtct gccagcgtcg gttctggcga
      901 tacgcccgat cattacaaac tcaactttat gcactttatt ccgcaagccg gtgcaatgaa
      961 tagcaatatg ctggcattaa aagagtggat tggttattgg aaagagaaat aaccgaagat
     1021 aaatagcagc aaggcaggtt ttcacctgcc ttctttttgc aaaaaatcag tcaaaaacaa
     1081 ccgcttgtct aactaatctc ttgtgggtca atgtcgatat tcagcctgat attattcgga
     1141 atgtttaaac tttccctttc ttgatcaaag gcatcgagta gctgttgtaa tgctcctcgg
     1201 agagggtgtt gaatcagcaa taaccagcgg taatgtcctg ctttcttcgc cattggggct
     1261 gaaaatggtg gagggagctg gaagccttgc ccgttttgtt gctggatttt ttggctaaaa
     1321 taatctgcta attgctcaag cagctgtaag gtttgttgat tatctttgcc ggtcgctttg
     1381 aacaaaactt gcgaggaaaa tggtggcaaa ctcattaatt gcctcatttt taacgcctca
     1441 tcagcaaaag ctcggtagcc ttgttccagc aaggttttaa gcagtgggtg atcagggtaa
     1501 tgggtttgta gcaccacttc gccttttttc tccgctcgcc ctgctctgcc tgccacctgt
     1561 atgtaaagct gtgctaattt ttcttccgca cgaaaatcag ttgaaaataa cgctgaatcc
     1621 acattaacaa tcgccaccaa cgtcacattc gggaaatgat gcccttttgc caacatttgc
     1681 gtaccaatca aaatttggct tttgccctct cgaatagcgg tcaaatggtt ttccaatgca
     1741 cctttgcgag cggtagaatc tctgtctata cgagtcactt ggtaatgagg gaattgctga
     1801 tttagcacct gctcaagttg ttctgtgccg acaccggtag ttattaaatg ggttgaacca
     1861 caatgtccgc attggcgggg aattaccctt tgggtggcac aatgatggca acgcaacact
     1921 ttttgctttt ggtggtaagt aaaaggctta ttacacgcct cgcactcaca aacccaaccg
     1981 cattcgtgac aaagtaatac cggggcaaag ccacggcgat ttaaaaacag catcacttga
     2041 ttgccttgct ctaactgggt tttcatcatt gaaagcaagc gctccgacaa accggcagtt
     2101 acccgttgcg tttttaaatc aataatctgc tgattggcaa gttgtgcatt gccggcccga
     2161 gcggttagtg tcagttcgat aaatttcccg ttttgcacat tgtgcaaact ttcaagactt
     2221 ggcgttgccg agcccagcac aatcggaata tttgcatttt tcgccctaac caccgccaaa
     2281 tcccgtgcgt ggtaacgcca accgtcttgt tgcttaaacg agctatcgtg ttcttcatcc
     2341 aagataatca cgcccaattt ttgaaactgg gtaaataaag cagagcgagt gccgattaca
     2401 atcgcacttt ctccattttt cgcccgcaac cacgcattta accgctcagt ttcgttcata
     2461 ttcgagtgca atacgtcaat ttcaacgtta aagcgagctt taaagcgttg taccgtttgc
     2521 ggtgttagcc cgatttcagg cacaagcagc agcacttgtt cctctcgctt aagcaccgtt
     2581 tcaattaact gcaaatacac ttcggttttg cccgagcctg ttacgccatt gagtaaaaag
     2641 ctcgcaaaag tggtttgtgc atttaaacgg ctgaccacta aggtttgttg tttatttaag
     2701 atcagccgat tgttagtatt tactaacgga aattcaccta gtgagtcttg ccacgcttgt
     2761 gccataaacg gcacatcaat tttctcgata tacccttttt cgagtaagcc tttccaagca
     2821 gatgggctac aagcggtcgg tttttcaaaa tattttgcac attcggcaag ctcggttaac
     2881 cgttccacct gctttttggc ttttttattt tcaccgataa tcagagattt ccgcccttgc
     2941 tccgtcacca caaaataatt cggttgaact cgctcagtgg aatcgccatt gcgtaatttt
     3001 atcggtaagg ctccggttag cacttcacca atcggggcgt ggtaataacg agccgcccag
     3061 ttcagcagtt gccacatttc tgagtcaaaa atcgggctag aatccaacgc tgctttaatc
     3121 ggtttgagtt tttcggttgg cacatcacta gtttgcggga aatcgaccac aatgccgatt
     3181 ttggtctggc gaccaaaagg cacaaccacc cgcccgcctt ggcaggcatt gaatgccggc
     3241 ggtagcaaat aatcaaaata acgatgtaac ggcacattca gtgccactct gattaatctc
     3301 acttctctaa accaattttg attgcaagcc cgataaatat cgagccgcag attttattta
     3361 accaatttcc aaaggtttga ttatgccgta aaaaacgccc aacaggtgca gcggctaaga
     3421 ctaaaatact tacccaaata atgctggtgg ataaaaacgt tagccccaac agcataaaag
     3481 gtattttcca aattactgga tacaaactgc ggcaataacg ccaagaaaaa gagcaatact
     3541 ttcgggttta atagattggt aatcaaccct tgacggaata tcttccatag aggaagtttg
     3601 tctagattga ttttctctaa caccaacggc tctcgccaca ttttaatgcc gagatagcag
     3661 agataaattg cccctgcgta tttcagaaaa ttaaataaag tgggcgaatg tgccacaatg
     3721 cctgcaagcc cgagtgaaac cgcaagaata tggcataaaa tgcctgctag aatgccgaat
     3781 gaagacatta agcccgactt tttcccctca gcaatagtgc gagttaaaat atagatagta
     3841 tccgaacccg gtgtaagatt tagcagaata caagcggtca aaaatcccca ataatttaca
     3901 atatcactca ttattcacac tcaatttacg tttacgctgc tgacgaagag caaacatacc
     3961 atctaatact aaaaatgaaa tggagatcaa caagcaaatc atcaacaagt aagattcatt
     4021 tgctaacctt tcgccaatta agaaagacac caccaacatc aatagtggct caacatagcc
     4081 taataaacca agcaaattaa agggtaaaat cgtgctggct agcgtatagc tgataagtgc
     4141 agtgccacta attaaaccca gtaatgcaat aaaatagtaa atattgaggt tttgtgtttc
     4201 gacatattgc atatcggttt gagagataaa ataacaagct acagggagca aatataaaat
     4261 ttctaccaca aagctatgta aatggcttaa gccaaacttt cggcgaaggt aaaaatagat
     4321 tgggtaacct gtgcaaacaa ataaacttgc tatcgaaatt tttcccgtaa acaaaatatt
     4381 actcgctaca ccaatgaaag caaccacaat cgccaaccat ttattcaagg aaagcgtttc
     4441 tttataaacc actttgccaa atgccaccat aataatcggc attaatagat agcccattga
     4501 aacctcaatc gcttttccgc tattgggagc ccataaaaac agccacatct gcccgcccac
     4561 aatagatgcc gtgattaaaa taaccagcaa taaataaggc tcttgcttta ggcgttgaag
     4621 aaatacccaa aactcatttt gttttttaaa taagtataaa gcaaggaaaa gaaaaggaat
     4681 cgtaaccagc atacgaatgc cgaaaatgct cacacctgcc agcggtttta gaaagatcgc
     4741 caaatagtac atacagccga ataacactga ggctgaaaga gataaaagaa tgccttttaa
     4801 catagtgata agaaagtaat tattttttaa tttttcaaat ctcccctacc cctcttttct
     4861 aaagaggatt gtcgagttct aaataaatga ataatgttcg gaggtagggg cgggtccagt
     4921 gcccgcccgc aaattaatat cgttagcgga caggcacagg acctgtccct actgatatta
     4981 tagaacttac aaccctgctt ttataaagtg gggtaaaggg agattcgact tagtcttgac
     5041 gataggtttt caagaaattc gcaaggcgac caatcgcttc ttcaatttga tgtgcataag
     5101 gcaaagttac cacacggaag tgatccggtt ttttccagtt aaaaccggaa ccttgcacta
     5161 atagcacttt ttcttgtcgc aacaaatcgt agatgaattt ttggtcatct ttaatgccgt
     5221 acatttcggt atcaattttc gggaacatat ataacgcccc tttcgctttt acacagctaa
     5281 tacccggaat ttgtaccaat aattcgtgca ttttatttcg ttgttccagc aaacgaccgc
     5341 ccggcaacac aaattcattg atactttgat aaccacctaa tgcggtttga atggcgtgtt
     5401 gcatcggcgt attggcacat aaacgcatag aagagagcat atccaaccct tcaataaaac
     5461 ctttggcttg atgcttcgga ccgctcagca ccatccagcc ctgacggaaa ccggcaacac
     5521 gatacgcctt ggataaaccg ttataagtta cggttaatac gtccggtgca agagcagcaa
     5581 tatggtgatg taccgcacca tcatagacaa ttttttcata aatttcgtca gcaaaaataa
     5641 ttaagttatg ctgacgggca agttctgcaa tttccagcaa aatttgacgg ctatacactg
     5701 ctccggtcgg gttattcggg ttgataacca aaatcccttt agtgcgaggc gtgattttcg
     5761 atttaatatc ttcaagatca gggaaccact cattttcttc atcgcacagg taatgcactg
     5821 ctttaccacc ggctagggtt gaagctgctg tccataacgg ataatcaggc attggaatta
     5881 gaatttcatc gccatcattt aataacgcct gcatcgacat tgtaatcagt tcagatacgc
     5941 cgttgccgat gtaaacatcg tttacatcca taccacgcat accttttgat tgataatatt
     6001 gcacaatcgc tttgcgggca gaatacaaac cttttgaatc acaatagcct tgtgcggttg
     6061 gaagattacg aatcacatcc actaaaattt catcgggtgc ttcaaagcca aaaggggctg
     6121 ggttaccgat atttaatttt aaaattttat gcccttcttc ttctaagcga agtgcctctt
     6181 tatgaattgg cccacggata tcgtaacgaa cctgagctaa cttatctgat ttgggaaaac
     6241 gatccattgt aaacttccta taatgtatta tctaattgta gtctcatccc gctgttcaaa
     6301 aaatcggtaa ttcggaatga atttgtaaac attactctaa tttgggagga aaaaaaagag
     6361 gaaatacgtt aaaatggaga cctttttttc aatcagctaa aaacaagcgg tttctttttc
     6421 tgattttttt gcaaatgctt atttttcatc aattaaaaca agaacttaca aaaagttact
     6481 caaaagtggg aagagagcaa ggattggtcg ccattcaagc ctctatgccg ctattggagg
     6541 caaatattcc gctactcggt tggttgaaaa cacaaaaaac aagctaccct cacttttttc
     6601 tgcaatatcg tgatacagat cagaccattg ccaccattgg cacacttcgc caattttata
     6661 cgctagaagc tgcacaatct tttattcaac aacatcattt cccgttaatc ggcgggcttc
     6721 aatttgaagg caatacgcaa tttattttgc ctcaattttc attggtaaaa aatcagcaaa
     6781 atttaaccgc ttgcttctat tttgatagtg ataactttga acaacaagcg gttatttttg
     6841 aacaatttct tgcaaatttc agccaaatta ccgaattagg actaacggat aatccgctcg
     6901 tttcaatgag tgccaccagc cattttgagc aatggaaagc caatattgaa aaagcgattg
     6961 tttctattga gcagggcgat tttcaaaaag tggtattggc aaatgcgatg agacttcatt
     7021 ttgagcaacc catttgtgct tacagcttgc tggcacaaag ccaaaaaaca aatttaggtt
     7081 gttaccactt tttatgggcg gaaagtgctg atgttgcctt tcttggctca acccctgagc
     7141 ggctatatca acgccaaggg ctacactttt ctaccgaagc cctcgccggc accgctgcgg
     7201 ttaccaccag tgaagtgcaa accgaactca atgcacaatg gctgttaagt gatcccaaaa
     7261 atatctatga aaatcaatta gtggttgatg acattgaaaa ccaactcaaa gattgcgtga
     7321 ccgaatttgc agtatccgag gcaaaaatta agcgtttgca taatgtgcaa cacctacgcc
     7381 gccaaattca agcaacgctc aaaccggaca cctcagattc agattgctta agcaaaatcc
     7441 acccaactgc ggcagttgca gggcttcccc gccaaaaagc aaagggtttt attaccgaaa
     7501 atgagccttt tacccgccat tggtatgcag gtacattagg cgtaatgggc aacgaggaag
     7561 ccgaattttg cgtaacactc cgttctgcat taatttcaca aaattcaatt acgctttatg
     7621 ccggtgccgg cattgtgtca ggctcagatc cacaatcgga atggcaagaa attaaacgta
     7681 aggcacaagg aatggcaaaa ctgttggatg ttgatttagt aacaaaaggg gaactgtaag
     7741 ctaggctttc agaacttaat ctcttaatta tttctttcta tattttcatt tccaacttcc
     7801 caacaaaata tattattgcc accaaattag tagtgaatat tccataatta aaaaatttgg
     7861 ggctgtagta gattagcatc actgctatac tacaaaaatg aagataaccc attgtaaatt
     7921 aaagaaatct ttgcagaaaa agcttcttga attttttgtt gctgaagtga ccgctagaac
     7981 cgctgctgat ttattaggta ttcaacccaa tacggcagcc ctgttttacc acaaaattag
     8041 gctggtcatt gaacaccatt tagcccttga agctcatgaa atttttgagg gaaaaattga
     8101 ggtagatgaa agctattttg gtggtcatcg caaaggaaaa cgagggcgag gtgcggctgg
     8161 gaaagtcgct gtattcggtt tgttgaagcg tcaaggaaag gtgtttacaa aataccaaat
     8221 cagaaacgct aatgccgg
//
LOCUS       DANLYI010000100         8189 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.100, whole genome shotgun sequence.
ACCESSION   DANLYI010000100 DANLYI010000000
VERSION     DANLYI010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 8189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8189
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.100"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            complement(<1..626)
                     /locus_tag="PXD81_001570"
     CDS             complement(<1..626)
                     /locus_tag="PXD81_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5537584.1"
                     /translation="MAKQIFKQAPLPFVGQKRMFLAQVSQILNENITDDGQGWTIIDV
                     FGGSGLLAHTAKHIKPKAHIIYNDYDGYAERLKHIPDTNRLRKQIYDIIGKSTPKNKR
                     LDPDKKSQVINIIQSFDGYIDVNCVASWLLFSGQQINSLEDLFNKIFWNGVRQTDYPS
                     AEGYLDGIEVTHESFHKLLPRFQHKDKVLLLLDPPYLCTRQESYKQAT"
     gene            complement(673..798)
                     /locus_tag="PXD81_001571"
     CDS             complement(673..798)
                     /locus_tag="PXD81_001571"
                     /inference="COORDINATES: protein motif:HMM:NF021626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Com family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL5537585.1"
                     /translation="MQSLRLIEIRCKYCQKLLAKAKDVQHLEIKCVRCKSINKFN"
     gene            complement(945..1154)
                     /locus_tag="PXD81_001572"
     CDS             complement(945..1154)
                     /locus_tag="PXD81_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5537586.1"
                     /translation="MFRNELQVIDGKRYIVLECQFRREWRVAIESRGTVTNGEAIEIC
                     QYWVKYKGVKPEQLKIVEVPDILKE"
     gene            complement(1157..1450)
                     /locus_tag="PXD81_001573"
     CDS             complement(1157..1450)
                     /locus_tag="PXD81_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5537587.1"
                     /translation="MSESFSLEKALWEEPRPRLQLDLPCPVIKLEVGKRYYTINIHGV
                     DCYDGDMSFTEIDEWTYESIDDVQTKSRAEQGLLFASEADAQEWIDAMRNSSR"
     gene            complement(1443..1880)
                     /locus_tag="PXD81_001574"
     CDS             complement(1443..1880)
                     /locus_tag="PXD81_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5537588.1"
                     /translation="MKTTQHILDEREQQHGNYDSFAKIYGGLRKVSDSHAEKLTWPQQ
                     IAVEMMLFKIARILNNGANHQDNYQDIAGYAMLGGGLYNPNVSAEVKALPKPLTDSIY
                     PESHLNKSSVWRLDLEFETKEEATEVLEALTGKSAGVSEVNNE"
     gene            complement(2119..2796)
                     /locus_tag="PXD81_001575"
     CDS             complement(2119..2796)
                     /locus_tag="PXD81_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein/antirepressor Ant"
                     /protein_id="HDL5537589.1"
                     /translation="MSNQVQFPVFNFNSSAVRVIIDPNQEPWFCGADVCRILGYVNES
                     LTLQKHCKENGVSKRYLTDKMQRQQKAIFINEPNLYRLIIKSRKPEAEKFEAWVFEEV
                     LPQIRKTGKYALQNNQQNLPLAPPPKKYSFDFTEDELQSLIWAWFAFVRGIHTFRYIY
                     PMFQKLGSNIAPEIYGQGFEYSHTAQSAHKILERITREFDCDPMTNWRVLKHLRGFDP
                     AFKKPTF"
     gene            complement(3044..3883)
                     /locus_tag="PXD81_001576"
     CDS             complement(3044..3883)
                     /locus_tag="PXD81_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HDL5537590.1"
                     /translation="MANSVMIVQGVEVKVTARDGEDYISLTDMCKAFGDSDQLIKNWL
                     QNKNTIEFLQVWEELNNPNFNLVELHQIKNNIGLNRFVMSVKKWTATGAIGLVAKAGR
                     YGSGTYAHKDIALEFGSWLSPEFKLYLIKEFQRLKQQEANESKLEWSVKRILSKANYR
                     IHTDAIKAHIIPALLNTKQHTFVYSSEADILNQALFGQTAKQWKDRNPNLKGNMRDHA
                     TVEQLTVLAALESQNALLIEQGYSQEERLAMLNRLAIQQMSSLLQTRAIEELKEKPLL
                     IEE"
     gene            4113..4490
                     /locus_tag="PXD81_001577"
     CDS             4113..4490
                     /locus_tag="PXD81_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5537591.1"
                     /translation="MFSLLRDALTFAAMFTGILFPAIIHKYDLSLGTQIQLLSFVPAF
                     FVLCHFLIKRKYQRDFDRLSYLSAVVCVAMCAGVGAFSQSEMIELGFKVERVLDYVTI
                     KFLLYFWSLLLLPVVLRKLFGKD"
     gene            4622..4810
                     /locus_tag="PXD81_001578"
     CDS             4622..4810
                     /locus_tag="PXD81_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5537592.1"
                     /translation="MKELFKQWLINQDSPFINSCGVECILSKVDDWLNIINANEEETE
                     TLVEWLNAFLQEVSVFVA"
     gene            complement(4814..5311)
                     /locus_tag="PXD81_001579"
     CDS             complement(4814..5311)
                     /locus_tag="PXD81_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HDL5537593.1"
                     /translation="MQQLIKNIEQWAEDRNLINGSTPQKQMLKLMEEFGELCAGVATN
                     EFETILDSLGDCLVICTIMRKQLKMEKLFSISHCDNTNFNCGVAAQIVQANIDLGNVA
                     SELSRGCDLSRLEDYLSGFENQLDLIAQFYKTDVQTALLYAYIQIKNRKGKMIDGVFV
                     KAEDL"
     gene            complement(5295..5495)
                     /locus_tag="PXD81_001580"
     CDS             complement(5295..5495)
                     /locus_tag="PXD81_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5537594.1"
                     /translation="MTDTKRLDFIKKHKMEIRYNGLSDEFVISNHAENSQHFRMARNA
                     DLRTAIDEAMKEMRGLNNATTN"
     gene            complement(5492..6010)
                     /locus_tag="PXD81_001581"
     CDS             complement(5492..6010)
                     /locus_tag="PXD81_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HDL5537595.1"
                     /translation="MNEDKFFSVDVSNDIHILNLHETLEQAKQSCLNGATEAYEFAGD
                     MDDHESYEAYDLPYAVYGVVLGRAKSDIRPLTDEERESELFGEVEQVIEPPTLVENNG
                     WISIEDKLPPIETDVLGLCDISGMQLILIVSRELADNEWYFLSVNQYGLDDDVIAVTH
                     WQPLPKLPKDNQ"
     gene            complement(6120..6923)
                     /locus_tag="PXD81_001582"
     CDS             complement(6120..6923)
                     /locus_tag="PXD81_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdQ family protein"
                     /protein_id="HDL5537596.1"
                     /translation="MDKSTIQQISTLAVAATKEVQTDFGTVMLPEGIKLQNLEPFQAH
                     RNQFRAAFSTQRFGSLIEYATANAQENAQCFIDQEKMSAEIVFDMGNREQAGHAKHRA
                     NLVMKKTAAYKALCEINGSRRSQRDFSDFLEDWGGYLTAYHHEDEISIKNAVQAVRKM
                     TIDYARNEEHELSDFAAKKSAMESVEAKSTLQLPTHLVFTCNPYNGLDTRSFTLRVQV
                     LTGSGEPVLTARLVQAEQIEEAIATEFAEKLSDALSETSIKVNIGTIEI"
     gene            complement(6993..7352)
                     /locus_tag="PXD81_001583"
     CDS             complement(6993..7352)
                     /locus_tag="PXD81_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5537597.1"
                     /translation="MAKTNVPEFLDELDCGIFKDKLATALSEVALGVLTHDKKGKVTV
                     EFSLDKMDSDSPSVQIQHKLSYVKPTKRGKSAEEDTTATPMYVHKGGALSATPEKTET
                     AVKESPSGLKALSKAAA"
     gene            complement(7388..7846)
                     /gene="ssb"
                     /locus_tag="PXD81_001584"
     CDS             complement(7388..7846)
                     /gene="ssb"
                     /locus_tag="PXD81_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDL5537598.1"
                     /translation="MAGINKVIIVGRLGNDPELRTMPNGDTTAKISVATSTEWTDKVS
                     GDKKQATEWHSIIAFRNLADIIGKYLKKGSQVYVEGKLRTRKWQAQDGTDRWTTEIIA
                     DQLQMLGSSSSGNSNNNWATEPAGNPPPTNPYNHVMTDSESRDFDDDIPF"
     gene            complement(7849..8046)
                     /locus_tag="PXD81_001585"
     CDS             complement(7849..8046)
                     /locus_tag="PXD81_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5537599.1"
                     /translation="MARKIDAGVEIVEVDKLFIAKFFYDGKLQHTTYPQYSRQNAIML
                     INRKIERFNVGRDKPIKLYKE"
     gene            complement(8030..>8189)
                     /locus_tag="PXD81_001586"
     CDS             complement(8030..>8189)
                     /locus_tag="PXD81_001586"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5537600.1"
                     /translation="FALLILLLGIVGRMDYDDHIQMQRYKCERNQGVWQVESNGNQYC
                     GGKYGTKN"
BASE COUNT         2133 a         1643 c         1608 g         2805 t
ORIGIN      
        1 taagtggctt gcttatagct ttcttggcga gtacaaagat atggcggatc aagtaataac
       61 aataccttat ctttatgctg aaaacgtggt aatagtttat gaaaactttc gtgagtaact
      121 tctattccat ctaaatagcc ttccgcactt ggataatcgg tttggcgaac accattccaa
      181 aatattttgt taaatagatc ctctaaacta ttaatttgct gtccgctaaa tagcaaccaa
      241 gaagccacac aatttacatc aatataccca tcaaaagact gaataatatt gataacttga
      301 gatttcttat caggatctaa tcgcttgttt ttaggcgtac tcttcccaat aatgtcataa
      361 atttgcttgc gtagcctatt tgtatcggga atatgcttta gccgctccgc atatccatca
      421 taatcattgt agatgatgtg agcttttggt ttgatatgtt tggcagtatg agctaataac
      481 ccactaccac caaatacatc tatgattgtc caaccttgtc cgtcatcagt aatattctca
      541 tttaaaattt gagaaacctg agctagaaac attcgtttct gacctacaaa cggaagtggg
      601 gcttgtttaa agatttgctt tgccatagtt ctcctatggc gttcaggcgt tcttaacgct
      661 cagacactca attcagttaa atttattgat tgatttacaa cgtacacatt taatttctaa
      721 atgttgtaca tctttcgctt ttgccaataa tttttggcaa tacttacagc ggatctcaat
      781 taatcttaat gattgcatat aaatacactt ttacttcatt ttttgataaa attccgccta
      841 cctagctagg tggcggcgta tggctatatg caagcgtgct ttgcgtagct ggcattttaa
      901 gtgttaccgc acttataatg tcgccgtttt taactcattt gtccttattc tttcagaata
      961 tctggcactt caacaatttt cagttgctca ggttttactc ctttatattt aacccaatat
     1021 tggcagattt cgattgcttc tccattcgtt acagtacctc tagactctat tgcaactctc
     1081 cattcgcggc gaaattggca ttcaagtacg atataacgtt taccatctat tacttggagt
     1141 tcatttctaa acatagctac ctactactat ttcgcattgc atctatccat tcttgagcat
     1201 ctgcttcact ggcaaatagt aacccttgtt cagctctgct ttttgtctgc acatcatcaa
     1261 tactttcata agtccattca tcaatttcgg tgaagctcat atctccatca taacagtcaa
     1321 cgccatgaat attgattgta taatagcgtt taccaacttc aagttttata acagggcaag
     1381 gtaaatctaa ttgcaatctt gggcgtggtt cttcccacag tgctttttct aaactaaatg
     1441 actcactcat tatttacctc actaacaccg gcacttttac cagttaaagc ttccaacacc
     1501 tcagttgcct cttctttggt ctcaaactcc aaatccagcc gccaaacaga gcttttatta
     1561 aggtgtgatt cggggtaaat actatcggtt aatggttttg gtaatgcttt aacttctgcc
     1621 gacacatttg ggttataaag tcctccacct aacattgcat aaccggcaat gtcttgataa
     1681 ttgtcttgat gatttgctcc gttatttaaa attcgggcaa ttttgaataa catcatctca
     1741 acggcgattt gctgtggcca agtgagcttt tctgcgtgtg agtcgctgac tttgcgtaaa
     1801 ccaccataaa tcttggcaaa actgtcgtag ttgccgtgtt gttgttcacg ttcatctaaa
     1861 atgtgttgag ttgttttcat cgtgaatttt cctttagaaa tagccgcttg cggaacaagc
     1921 ggttgggtta attagcgttg agccatacaa attttgccat cgcagtcgtt gcgtaactct
     1981 agatggtcag cagcaactgc aaaggcggtt aaaaagagta tgatttttgc gtatttcatc
     2041 gtctaatcct caaaatttgg gtgtggaaat ccgccgcttg atgtttcgca agcggtcggg
     2101 ttttataatt ttttgtgatt agaacgttgg ctttttaaat gccgggtcga aaccacgaag
     2161 gtgtttgagt acacgccaat tcgtcattgg gtcgcaatca aattctcggg taatccgttc
     2221 aaggatttta tgagccgatt gtgcggtgtg gctatattca aaaccctgtc cgtagatttc
     2281 aggtgcgata tttgagccga gtttttgaaa catcgggtag atatagcgga aagtgtgaat
     2341 gccacgcacg aaagcgaacc aagcccatat gaggctttgg agttcatctt cggtaaagtc
     2401 gaaagagtat ttctttggcg gtggtgcaag cggtaggttt tgctgattat tttgcaacgc
     2461 atatttacca gtcttgcgaa tttgtggcag aacttcttca aatacccacg cttcaaactt
     2521 ttccgcttct ggtttgcgtg atttgataat cagtcggtat aggtttggtt cgttgatgaa
     2581 gatcgctttt tgttgtctct gcattttatc ggtgaggtat cgtttcgata ccccattttc
     2641 tttacagtgt ttttgcagag taaggctttc atttacataa cctaaaattc ggcaaacatc
     2701 tgcaccgcaa aaccacggtt cttgatttgg atcgataatg acacgaacag cagatgaatt
     2761 gaaattgaaa acagggaatt gaacttgatt tgacattttt tgtacctttt ggatttaagt
     2821 atttaatccg atcattaagt gggtgatcgg ggttcaactt accctccaaa agatgggcgg
     2881 agcttattca gatgtattgt atatcgctct ctcgccccga tcatttcgga ttaaacctaa
     2941 aatttaggta caaaaaaaac gctttatatc ggaggcggaa accgcttttg gaaagtaagt
     3001 gtggttatct tagccgaaag ggcggttttt tgtcaattag attttattct tcaattagca
     3061 atggcttttc tttcagttct tcaatcgctc tggtttgtaa cagcgagctc atttgctgaa
     3121 tagccaaacg gttgagcatt gccagtcgct cttcttggct gtaaccttgc tcaattagta
     3181 gggcgttttg gctttccaat gcagcaagga cggtaagttg ctcaacggta gcgtggtctc
     3241 gcatattgcc tttcaggttc ggattgcggt ctttccactg ttttgccgtt tgtccgaaga
     3301 gagcttgatt gagaatgtcc gcttcgctgg aataaacgaa agtatgttgt tttgtattca
     3361 gcaatgccgg tatgatgtga gctttgatgg catcagtatg aatgcggtag ttcgccttgc
     3421 ttaaaatgcg ttttacgctc cattcgagct tactttcgtt cgcttcttgc tgtttgagtc
     3481 gttggaactc tttgatgaga taaagcttaa attctggact gagccaagaa ccaaattcga
     3541 gagcaatatc tttgtgggcg taagtgccgc taccatatcg tcctgctttt gctactagcc
     3601 caatagctcc cgttgcagtc cattttttga cagacatcac aaagcggttt aagccgatgt
     3661 tgtttttaat ttggtgtaat tccaccaaat taaaatttgg attattcagc tcttcccaaa
     3721 cttgaagaaa ttcaatggta tttttatttt gaagccagtt tttaatgagc tggtcactgt
     3781 ctccaaaagc tttgcacata tcagttaggc tgatgtaatc ctcaccatca cgagcggtta
     3841 ctttgacctc cacgccttgt acaatcatta cgctatttgc catttcgttt ctccgttttt
     3901 aggtacaaaa aaagccgttg gtggacggct tgaaagtgcg gttatcttaa tccgaattgg
     3961 gggcggtgtc aattagatta atccgtataa gtatggaacc aagttaaaga gtagaactac
     4021 aacacataac caaaaaatga aattcatatc ttgctcagtt ttgattttgt gtaaaatacg
     4081 cttaagtttg tttttcattt aggaaacctt taatgtttag tttattgcgt gatgctttaa
     4141 cctttgctgc aatgtttaca gggattttat ttcctgcaat tattcataaa tacgatctat
     4201 cacttggtac acaaatccaa ttacttagct ttgttccagc tttctttgtg ttgtgccatt
     4261 ttcttattaa gcgaaaatat caacgtgatt ttgacagact ttcgtattta tctgctgtgg
     4321 tttgcgtagc aatgtgtgca ggcgttggag catttagcca atcagaaatg attgagctag
     4381 gttttaaagt tgagcgtgtt cttgattatg tgactatcaa attcttactc tacttttgga
     4441 gcttgctgtt attgccagtt gtgctaagaa aattgtttgg gaaagattag ttggttattt
     4501 aaagagagct atttgtcaaa cctcaatatc tatttttgcg atctgcatcg aaaaaataaa
     4561 ttttcgttct gacagtaaaa cgctttcgta gtaatctcat aaaaaacata aggggaacac
     4621 tatgaaagag cttttcaaac aatggctaat caatcaggac tcgccattca ttaatagctg
     4681 tggcgttgag tgtattttgt caaaagttga tgactggcta aacataatca acgccaacga
     4741 agaagaaacc gaaacgcttg ttgagtggct aaatgctttt ctgcaagagg tttctgtttt
     4801 tgtggcttag gtactataaa tcttctgctt tcacaaacac gccatcaatc atttttcctt
     4861 ttcgattttt gatttgaata taagcataga ggagagctgt ttgtacgtct gttttgtaga
     4921 attgagcgat aagatcaagc tgattttcaa agccgcttaa gtaatcttct aatcgagata
     4981 aatcacaacc tctagataac tcacttgcta cattacctaa atcaatattg gcctgaacaa
     5041 tctgagccgc aactccacaa ttaaaattgg tattatcgca atggctaatg ctaaacagtt
     5101 tttccatttt gagctgttta cgcataatcg tacaaatcac tagacagtca cctagactgt
     5161 ctaaaatagt ttcaaattca ttggttgcta cacctgcaca taattcccca aactcttcca
     5221 ttagttttaa catttgcttt tgtggggtag agccgttaat taaattgcgg tcttccgccc
     5281 attgttcgat gtttttaatt agttgttgca ttgtttagtc ctctcatttc tttcattgct
     5341 tcatcaatcg ccgtgcgtaa atcggcattt cttgccatac ggaaatgctg gctgttttca
     5401 gcgtgattac taataacaaa ttcgtctgat aagccattgt agcggatttc cattttgtgc
     5461 tttttgataa aatctaagcg tttggtgtct gtcattggtt gtcctttggc agctttggaa
     5521 gtggttgcca gtgggttact gcaatcacat cgtcatcaag cccatattga tttacactaa
     5581 gaaaatacca ctcgttatcg gcaagttctc ttgaaactat taaaattaac tgcattcctg
     5641 aaatatcgca taaccctagc acatccgttt caataggtgg taatttgtcc tcaatggata
     5701 tccagccatt attttctaca agtgttggtg gttcaataac ttgttcaact tccccaaata
     5761 actctgattc tctttcttcg tcggttagtg ggcggatatc tgattttgct ctacctaaaa
     5821 caacaccata aactgcatag ggtaaatcat aggcttcata gctttcgtga tcatccatgt
     5881 cacccgcaaa ctcataggct tcagtagcac catttaaaca gctttgtttt gcttgctcta
     5941 aggtttcgtg cagatttagg atatgtatat catttgatac atccaccgaa aaaaatttgt
     6001 cttcattcat tttctttctc caaaatacca atagttgaga gaataataac cgcttgtatt
     6061 caacaagcgg tcaattttca ttaaaaactt acaagtaaac ttataagcta acttacaagc
     6121 tagatctcaa tcgtaccgat attgaccttg attgatgttt cgctaagtgc atcggatagt
     6181 ttttccgcaa actcagtcgc aatcgcctct tcaatttgct cagcttgaac taagcgggcg
     6241 gttaataccg gctcaccgct accggttagc acctgtacac gcagagtaaa tgatcgagtg
     6301 tctaagccgt tatatgggtt gcaagtaaat acaaggtgag ttggtagttg cagtgtagat
     6361 ttagcttcca ccgattccat tgctgatttt ttggctgcga aatcgctgag ttcatgttct
     6421 tcgtttcggg cgtagtcaat cgtcatttta cgcaccgctt ggacggcatt tttaatactg
     6481 atttcatctt cgtggtggta agcggtcaag tagccgcccc aatcttcgag gaaatcagaa
     6541 aaatcacgct gtgagcggcg tgagccatta atttcgcata gagctttgta ggctgcggtt
     6601 ttcttcatca ccaaattagc acgatgttta gcgtgaccgg cttgctctcg gttgcccata
     6661 tcgaatacaa tttccgcact cattttctct tggtcaataa agcattgtgc gttttcttgg
     6721 gcattggcgg tagcatattc aatcaggcta ccaaaacgtt gcgttgaaaa cgctgcacgg
     6781 aattgattac ggtgagcttg gaaaggctct aaattttgta atttaatgcc ttcaggtaac
     6841 attactgtgc caaaatcagt ttgaacctct ttagtcgcag ctacagcaag agtagagatt
     6901 tgttgaatag ttgatttatc cattttgagt tcctttttgt ttaagtaaaa ataaagccac
     6961 gcattaacgt ggcttggatt gaaaataatt gattatgcag cagctttaga gagagccttt
     7021 aaaccgctag ggctttcttt tactgccgtt tctgtttttt caggtgtagc agataatgcc
     7081 ccacctttat gaacatacat tggtgtggcg gtagtatctt cttcggctga cttaccacgt
     7141 ttggtcggtt tgacatagct caatttgtgc tgaatttgaa cagatgggct gtcgctatcc
     7201 attttgtcta agctaaattc aaccgtaact ttccctttct tatcgtgggt tagtacgcct
     7261 aacgccactt ctgaaagagc tgttgccagc ttgtctttga aaataccgca atctaattca
     7321 tctaagaatt caggtacatt tgtttttgcc attttgggtt ctcctaatta gggtttgaat
     7381 aaaattatta gaacggaata tcatcatcaa aatctcgtga ttctgaatcc gtcattacgt
     7441 ggttatatgg attggttggc ggtgggtttc ctgccggttc ggttgcccaa ttattattgc
     7501 tgtttccgct gcttgagcta ccaagcattt gtagctgatc agcgatgatt tcagtcgtcc
     7561 agcggtctgt gccgtcttgg gcttgccatt tgcgagtacg tagtttgccc tcaacataga
     7621 cttgtgagcc ttttttcagg tatttgccaa taatgtcagc taaattccga aaggcgataa
     7681 tgctatgcca ctctgtggct tgttttttat cgcctgatac tttatctgtc cattcggtcg
     7741 aagttgctac gctgattttg gcggttgtat cgccgtttgg cattgttcgc aattccgggt
     7801 cgttacctag gcgaccgaca ataatcactt tgttaattcc tgccattgtt attccttata
     7861 aagtttaatt ggtttatctc tgccaacgtt gaaccgctca attttgcggt taatcaacat
     7921 aattgcgttt tggcggctgt attgtggata ggttgtgtgc tgtaatttgc cgtcataaaa
     7981 aaacttggcg ataaatagtt tatctacctc aacaatctca acaccggcat caatttttcg
     8041 tgccatattt ccctccgcag tattgattgc cattactctc aacttgccaa acgccttgat
     8101 ttcgttcgca tttgtaacgt tgcatttgta tgtggtcgtc ataatccatt cgcccgacaa
     8161 tgcctaatag caaaattagc aaggcaaat
//
LOCUS       DANLYI010000101         8176 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.101, whole genome shotgun sequence.
ACCESSION   DANLYI010000101 DANLYI010000000
VERSION     DANLYI010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 8176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8176
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.101"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            23..1015
                     /locus_tag="PXD81_001587"
     CDS             23..1015
                     /locus_tag="PXD81_001587"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015701738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HDL5537601.1"
                     /translation="MKKSFILQQQEISFVKNTFTQYLIDKLGIIEVQGPILSEVGNGM
                     QDNLSGIEKAVKVNVKCIPSATYEVVHSLAKWKRHTLARFGFKEGEGLFVHMKALRPD
                     EDSLDRTHSVYVDQWDWEKVIPEGRRNFDFLKETVNEIYKAIRLTELAVEARFDIPSV
                     LPKQITFVHSEDLVQRYPNMTSKERENAICKECGAVFLIGIGGKLSDGKPHDGRAPDY
                     DDWTTESEQGYKGLNGDILVWNEELGTAFELSSMGIRVDETALRLQVALTGDEDRLEM
                     DWHKDLLAGRLPLSIGGGIGQSRLVMFLLRKKHIGEVQSSVWPKAVFEEFENIL"
     gene            complement(1139..1558)
                     /gene="atpC"
                     /locus_tag="PXD81_001588"
     CDS             complement(1139..1558)
                     /gene="atpC"
                     /locus_tag="PXD81_001588"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HDL5537602.1"
                     /translation="MASQFELTVVSAERKIFEGTVTSVRVSGTDGELGVYAGHAPLLT
                     AIKPGMVKFTLADGKEEFIYVSGGFLEVQPTVVTVLADTAIRGEELDEQRILAAKRKV
                     EETLSKTSDADMTAKLAREIAKLRVYELTKTKLANKR"
     gene            complement(1586..2959)
                     /gene="atpD"
                     /locus_tag="PXD81_001589"
     CDS             complement(1586..2959)
                     /gene="atpD"
                     /locus_tag="PXD81_001589"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011978979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HDL5537603.1"
                     /translation="MATGKIVQIIGAVIDVEFPQDAVPKVYDALKVESGLTLEVQQQL
                     GGGVVRCIALGSSDGLKRGLKVENTNKAIEVPVGTKTLGRIMNVLGEPIDEAGPIGEE
                     ERWTIHRAAPSYEEQANSTELLETGIKVIDLVAPFAKGGKVGLFGGAGVGKTVNMMEL
                     IRNIAIEHSGYSVFAGVGERTREGNDFYHEMKDSNVLDKVSLVYGQMNEPPGNRLRVA
                     LTGLTMAEKFRDEGRDVLFFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEMGVL
                     QERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDSTVVLSRNIASLGIYPAVDP
                     LDSTSRQLDPLVVGEEHYNVARGVQGTLQRYKELKDIIAILGMDELSEDDKRLVARAR
                     KIERFLSQPFHVAEVFNGVPGKFVPLKETIRGFKGILEGEYDHIPEQAFYMAGSIDEV
                     VERAKQM"
     gene            complement(2980..3846)
                     /gene="atpG"
                     /locus_tag="PXD81_001590"
     CDS             complement(2980..3846)
                     /gene="atpG"
                     /locus_tag="PXD81_001590"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011201492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HDL5537604.1"
                     /translation="MAGAKEIRTKIASVRNTQKITKAMEMVAASKMRKTQERMAASRP
                     YAESIRKAISHIAKGNIEYKHPFLTPRPVKKVGYLVVSTDRGLCGGLNINLFKTVLHE
                     LKEKDDQGVKSRLAVVGNKGISFFNPMGLEIKGHINGLGDTPAMEDLVGIVNGMVNAY
                     REGEIDEVYVVYNRFINTMSQKPTVQQLLPLPALENDSLEQTGSWDYLYEPNPQALLD
                     SLLVRYLESQVYQAVVDNLASEQAARMVAMKAATDNAGNLINELQLVYNKARQASITN
                     ELNEIVAGAAAI"
     gene            complement(3872..5413)
                     /gene="atpA"
                     /locus_tag="PXD81_001591"
     CDS             complement(3872..5413)
                     /gene="atpA"
                     /locus_tag="PXD81_001591"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HDL5537605.1"
                     /translation="MQLNSTEISELIKKRIAQFNVVSEAQSTGTIVSVSDGVIRIHGL
                     SDVMQGEMIALPGNRYAIALNLERDSVGAVVMGPYADLAEGMQVQCTGRILEVPVGRG
                     LLGRVVNTLGQPIDGKGEIQNDGFSPIEVIAPGVIDRQSVDQPVQTGYKAVDSMVPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTIANVVRKLEEHGAL
                     ANTIVVVASASESAALQYLAPYAGCAMGEYFRDRGEDALIVYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERASRVNADYVERFTNGAVKGQTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLESGLFNAGIRPAVNPGISVSRVGGSAQTKLVKKLAGG
                     IRTALAQYRELAAFAQFASDLDEATRKQLSHGQKVTELLKQKQFEPMSVAQLGLSLAA
                     AEYGYLDDIPVERVGSFEANLLAYAQSNYGEFMQELSKSGNFNDEIKDKLNEILSGFK
                     KNSAW"
     gene            complement(5426..5959)
                     /gene="atpH"
                     /locus_tag="PXD81_001592"
     CDS             complement(5426..5959)
                     /gene="atpH"
                     /locus_tag="PXD81_001592"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015674172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HDL5537606.1"
                     /translation="MSELSTVARPYAKAAFDFALEQGQLEQWQQMLTFSAAVANDEQV
                     ASFLNSSLASGQISETFIKICGEQLDQYGQNFIRVMADNKRLAVLPAVLNAFLELRAE
                     QESIKDVEVVSADKLTDAQEAKIANAMEKRLGTKVRITSSIDSSLIAGVIIRYDDVVI
                     DGSSRGQLNRLSQELSL"
     gene            complement(5973..6443)
                     /gene="atpF"
                     /locus_tag="PXD81_001593"
     CDS             complement(5973..6443)
                     /gene="atpF"
                     /locus_tag="PXD81_001593"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009874987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HDL5537607.1"
                     /translation="MNLNATLIGQLIAFALFVAFCMKFVWPPLIRVIEERQANIASAL
                     ASAEKAKQEQADSKAAADQEILRAKEEAQNIIDLATKRRNEILESVQAEAEVERQRII
                     EQGYAEVESERKRVQEELRQKVAALAIAGAEKIVGRSVDAAANNDIIDKLVAEL"
     gene            complement(6504..6758)
                     /gene="atpE"
                     /locus_tag="PXD81_001594"
     CDS             complement(6504..6758)
                     /gene="atpE"
                     /locus_tag="PXD81_001594"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HDL5537608.1"
                     /translation="MESVITATIIGASILLAFAALGTAIGFAILGGKFLESSARQPEL
                     ASSLQTKMFIVAGLLDAIAMIAVGISLLFIFANPFIDLLK"
     gene            complement(6876..7664)
                     /gene="atpB"
                     /locus_tag="PXD81_001595"
     CDS             complement(6876..7664)
                     /gene="atpB"
                     /locus_tag="PXD81_001595"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005713638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HDL5537609.1"
                     /translation="MAGTTAEYISHHLSFLSTGEGFWAVHLDTLFFSLVAGAIFLWVF
                     SRVAKNATTGVPGKLQCFVEIVIEWVNGLVKDNFHGPRNVIAPLALTIFCWVFIMNAI
                     DLIPVDFLPQLANLFGIHYLRAVPTADISATLGMSICVFALILFYTVKSKGFGGLVKE
                     YTLHPFNHWAFIPVNFILETVTLLAKPISLAFRLFGNMYAGELIFILIAVMYMADNFA
                     LQALGIPLHLVWAIFHILVITLQAFIFMMLTIVYLSIAYNKADH"
     gene            complement(7687..8070)
                     /locus_tag="PXD81_001596"
     CDS             complement(7687..8070)
                     /locus_tag="PXD81_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HDL5537610.1"
                     /translation="MSAVINRAKSQYKQALKVEFFLLCVFALGVSLWKMNFNFSFLGG
                     ALASFLPHLVFVYWVFFKKSAKNQSKMTAFYRGEGMKWLVTIILVIGCFKLIPALDVV
                     LFFVGYFIALFLNNMIPFVLSKHHH"
BASE COUNT         2425 a         1840 c         1531 g         2380 t
ORIGIN      
        1 tctttaatta ggaaagcaaa caatgaaaaa atcttttatt ttacagcaac aagaaatcag
       61 ttttgttaag aacacattta ctcaatattt aattgataaa ttaggcatta ttgaagttca
      121 ggggcctatt ttgagcgaag tcggcaacgg tatgcaagac aacctttccg gtatcgaaaa
      181 agcagtaaaa gtaaacgtaa aatgtattcc aagcgctact tatgaagtgg ttcattctct
      241 tgcaaaatgg aaacgccaca ccttagctcg ttttggattt aaagaaggag aagggttatt
      301 tgttcatatg aaagctcttc gccctgatga agattcttta gatcgcacac actcggtata
      361 tgttgaccaa tgggactggg aaaaagtcat tcctgaaggt cgccgcaact tcgatttctt
      421 aaaagaaacg gtaaacgaaa tctataaagc aattcgttta accgaattag cggtggaggc
      481 tcgtttcgat attccatctg tattgcctaa acaaattact ttcgttcaca gcgaagactt
      541 agtgcaacgc tatccgaata tgaccagcaa agagcgtgaa aatgctatct gtaaagagtg
      601 cggagcggtg ttcttaatcg gtatcggcgg caaattatca gacggtaaac ctcacgatgg
      661 acgagctccg gactatgatg actggacaac cgagtccgaa caaggctaca aaggcttaaa
      721 cggcgatatt ttagtttgga acgaagaact cggcacagcc tttgaacttt cctcaatggg
      781 tattcgtgta gatgaaaccg ccttacgctt acaagttgct ttaactggcg acgaagaccg
      841 cctcgaaatg gattggcaca aagatttatt agccggcaga ctcccgcttt ctatcggcgg
      901 cggtatcggt caatctcgct tagtgatgtt cttactacgc aaaaaacaca tcggtgaggt
      961 gcaatccagt gtctggccaa aagcggtatt tgaagagttt gagaatattc tttaataaaa
     1021 caaaaaagcg aaccttaggg ttcgcttttg ctttctacaa gcggtcttat tttacaaaaa
     1081 atttactgtt tttaaccgct tgtaaaacaa aaccccactt tcgtggggtt ttttgatatt
     1141 aacgtttatt cgctaatttg gttttagtga gttcgtaaac acgcagtttc gcaatttctc
     1201 tcgctaactt cgcggtcata tccgcatcgc tggtttttga aagtgtttct tctactttgc
     1261 gttttgcggc aagaatacgc tgttcgtcta gttcttcacc acgaattgcg gtatcagcca
     1321 acactgttac tacggtcggt tgtacttcaa ggaaaccacc tgaaacgtag ataaactctt
     1381 ctttgccgtc agctaaagtg aatttaacca ttcccggttt aattgcggtt aataacggtg
     1441 catgccctgc atacacccct aattcaccat ctgtccctga aacacgcacg ctggtaactg
     1501 tgccttcaaa gattttacgt tctgcactca ctaccgtgag ttcaaattga gatgccatgt
     1561 tgttctcctt tagtttagaa caaaattaca tctgttttgc tctttcaacc acttcgtcaa
     1621 ttgaacccgc catatagaac gcttgttccg gaatatggtc gtattcgcct tctaaaatgc
     1681 ctttaaagcc acgaattgtt tcttttaacg gtacgaattt gcccggtacg ccgttgaata
     1741 cttccgcaac gtggaatggt tgtgaaagga aacgttcgat tttacgcgca cgagcaacaa
     1801 ggcgtttgtc gtcttccgat aattcatcca tacccaaaat tgcaatgatg tcttttaact
     1861 ctttataacg ttgtaatgta ccttgtacac cacgagcaac gttatagtgt tcctcaccaa
     1921 ccacgagtgg atctaactga cgagaggttg agtcaagagg gtcaaccgcc gggtaaatac
     1981 cgagtgaagc gatattacgg cttaatacca ccgttgagtc taagtgggca aaggtagttg
     2041 ccggagacgg gtcagttaag tcatccgccg gaacatatac cgcttgcaca gaggtaatag
     2101 agccggtttt ggttgaggta atacgctcct gtaatacgcc catctcttct gcaagagtcg
     2161 gctgataacc taccgcagac ggcatacgac ctaataacgc agatacttcg gtaccagcta
     2221 aagtataacg gtagatgtta tccacgaaga ataatacgtc acgaccttca tcacggaatt
     2281 tttctgccat ggttaagccg gttaatgcca cacgcagacg gttgcccggt ggttcgttca
     2341 tttgaccgta aaccagcgat actttgtcta atacgttaga gtctttcatt tcgtggtaga
     2401 agtcattacc ctcacgagta cgctcaccta caccggcgaa taccgagtaa ccgctatgtt
     2461 cgatagcgat gttacggatt aactccatca tatttacggt tttacctaca cccgcaccac
     2521 cgaataagcc aactttacca cccttagcaa atggagcaac taagtcgata actttgatac
     2581 cggtctctaa aagctcggtg ctgttcgctt gttcttcgta gcttggtgct gcacggtgga
     2641 ttgtccaacg ttcttcttca ccgattgggc ctgcttcatc aattggctca cctaatacgt
     2701 tcataatacg accgagtgtt tttgtaccaa ccggtacttc aatcgcttta ttcgtatttt
     2761 caacttttaa gccacgtttt aaaccatcag atgaaccaag tgcgatacaa cggaccacac
     2821 cgccacctaa ttgttgttga acttcaaggg ttaatcctga ttcaactttt aatgcatcat
     2881 aaacttttgg tactgcatct tgcggaaact caacatcgat taccgcaccg atgatctgta
     2941 caatttttcc cgttgccatt accgttcctc tattcattgt taaattgctg cggcacccgc
     3001 gacaatttca tttaattcat tcgtaatact tgcttgacga gctttgttat acactaactg
     3061 taactcatta atcagattac ctgcgttatc ggttgctgct ttcattgcca ccattcgagc
     3121 agcctgttca gacgcaagat tatctaccac tgcctgataa acttgagatt ctaaataacg
     3181 aaccagtaag ctgtctaata acgcttgtgg atttggttca tagagataat cccaagaacc
     3241 agtttgctct aatgagtcat tttccagtgc aggcaaagga agcaactgtt gtactgtcgg
     3301 tttttgtgac atcgtgttta taaaacggtt atataccaca tacacttcat caatttcgcc
     3361 ttcacggtag gcatttacca taccattaac aataccgact aaatcttcca ttgccggtgt
     3421 atcacccaat ccattgatat gacctttaat ctctagcccc attgggttaa aaaaggagat
     3481 ccctttattt cccaccacag caagtcgaga cttaacacct tggtcatctt tttctttcaa
     3541 ttcatgtaaa acggttttaa ataaattgat atttaagcca ccacataaac cgcgatcggt
     3601 tgaaactact aaatagccaa ctttttttac cggacgtggg gtcaaaaatg ggtgtttata
     3661 ctcaatgtta cctttggcaa tatggctaat tgccttgcga atactttcag cataagggcg
     3721 agaagccgcc atacgctctt gggttttacg catttttgat gcggcaacca tttccatcgc
     3781 tttggtaatt ttttgtgtat tacgaacact tgcaattttg gttcttatct ctttagcacc
     3841 tgccatatta tttctccgct aaaaagttag attaccaagc actgttcttt ttgaaaccag
     3901 ataaaatctc gttcaattta tctttaattt catcgttgaa attgccggat tttgacagtt
     3961 cttgcataaa ctcgccgtag ttactttgag cataagctaa taagtttgct tcaaatgagc
     4021 caacacgctc aaccggaata tcatctaagt aaccgtattc agctgctgct aatgataaac
     4081 ctaattgtgc aacagacatc ggctcgaatt gtttttgttt caacaattca gttacttttt
     4141 gaccgtgtga aagctgttta cgtgttgctt catcaagatc tgatgcgaac tgggcaaatg
     4201 ccgctaattc acggtattga gcaagtgcgg tacggatacc accggcaagc ttcttaacca
     4261 gtttagtttg tgcagaacca cctacacggg aaaccgagat acccgggttt accgcagggc
     4321 gaataccggc attgaataag cctgattcta agaaaatctg accatcggta attgaaatta
     4381 cgttagtcgg tacgaaggct gaaacgtcac ctgcttgggt ttcgatgatc ggtaatgcgg
     4441 ttaaagaacc ggtttgacct ttcacagcac cgttagtgaa acgttctaca tagtctgcat
     4501 ttacacgaga cgcacgctct aataagcgag agtgtagata gaacacatca cccgggaatg
     4561 cttcacgacc cggtggacgg cgtaataaca atgaaatttg acggtaagca actgcttgct
     4621 tagacaaatc atcgtaaacg atcaacgcat cttcaccacg gtcacggaag tattcaccca
     4681 ttgcacaacc tgcataaggt gcaagatatt gtaatgcagc agattcagac gcagaagcca
     4741 ctacaacgat agtgttcgct aatgcaccgt gttcttctaa tttacgcacc acgttggcga
     4801 tagttgatgc cttttgaccg attgcaacgt agatacattt aatgcctgaa tcacgttggt
     4861 tgatgatcgc atcaatcgct aatgcggttt taccggtttg acggtcacct ataatcaatt
     4921 cacgctgacc acgaccgatt ggtaccattg agtccaccgc tttgtaaccg gtttgaaccg
     4981 gttgatcaac cgattgacgg tcgataacgc ccggtgcgat cacttcgatt ggtgagaaac
     5041 cgtcattttg gatttcgcct ttaccatcga ttggctgacc taaggtattt acaacacgac
     5101 ctaataagcc acgaccaacc ggcacttcta aaatacgacc ggtacattgt acttgcatac
     5161 cttctgctaa atcggcataa ggtcccatta caactgcacc aacagaatct ctttctaagt
     5221 ttaatgcaat cgcataacga ttgcccggta atgcaatcat ttcaccttgc attacatcag
     5281 ataagccgtg gatacgaata accccgtcac ttaccgaaac gattgtccct gtgctttgag
     5341 cttcactcac cacattaaac tgggcaatac gttttttaat caattcacta atttcagttg
     5401 aatttagttg cattttttat tcctcttaca agctcaactc ttgactcaag cggtttagct
     5461 gtccacgact actaccatca atcactacat catcataacg aatgatcaca cccgcaatta
     5521 atgaactatc tattgatgaa gtaatacgta ctttcgttcc gagtcgcttt tccatcgcat
     5581 tggcaatttt tgcttcttgt gcatctgtta atttatctgc agaaacaacc tcaacatctt
     5641 taattgattc ctgttctgct cttaattcta aaaatgcatt caataccgca ggtaatactg
     5701 ctaaacgctt gttatcagcc attacgcgaa tgaaattttg cccatattga tctaattgtt
     5761 cgccacaaat cttgataaac gtctcagaaa tctgaccgct tgcaagtgat gagttaagaa
     5821 aactagctac ttgctcatcg ttagcgacgg cggcagaaaa agttaacatc tgctgccatt
     5881 gttccaattg accttgttct aaagcaaaat cgaaagccgc tttagcatag gggcgagcta
     5941 ctgtgcttaa ttctgacatc tgcccctcct agttatagtt ccgcaactag cttatcaata
     6001 atatcgttgt tcgcagcggc atccaccgaa cgacccacga ttttttctgc accagcaata
     6061 gccaatgcag ccactttttg acgaagctct tcttgaacgc gtttacgttc gctttcaact
     6121 tccgcgtaac cctgttcaat aatacgttga cgttccactt cagcttctgc ttgtacagat
     6181 tctaaaattt cattacgacg ttttgttgct aaatcaatga tattttgtgc ttcttctttc
     6241 gctctaagga tttcttgatc cgcagccgct ttagaatcag cttgttcttg tttcgctttt
     6301 tcggcagaag ccaatgcgct agcaatattt gcctgacgct cttctatcac tcgaattagc
     6361 ggtggccata caaacttcat acagaacgca acaaaaagtg cgaacgcaat cagttgacca
     6421 attagtgttg catttaaatt cacaacgccc tcctaaaagc cggtaaagtt attcaactga
     6481 ataacggtta atcaaatccc gacttatttc aataaatcaa tgaacgggtt tgcgaagatg
     6541 aatagtaatg aaatacctac agcgatcatt gcgatagcat ctaaaagacc agcaacgata
     6601 aacattttag tttgtaagct tgatgctaat tccggctgac gtgcagatga ctctaagaat
     6661 ttgccaccta agatagcaaa gccgatagca gtaccaagag cagcgaaagc taataaaatt
     6721 gaagcaccaa taattgttgc tgtgattact gattccataa tgttctccat tagattgagg
     6781 aattagcccg aaggctgggt tgttgataaa aatacatatc aagcggtcaa tattttgcaa
     6841 aaatttgcga aaaacctacc gcttgtaagg ctagtttaat gatccgcttt gttataagcg
     6901 atactcaaat aaacaatcgt tagcatcata aaaataaagg cttgaagcgt aataactaaa
     6961 atatggaaaa tcgcccaaac caagtgtaat ggaatcccca acgcctgtag agcgaagttg
     7021 tctgccatat acattactgc aataaggata aagattaact cacctgcata catattaccg
     7081 aataaacgga acgccaatga aatcggtttt gccaaaagcg ttaccgtttc aagaataaag
     7141 tttaccggaa tgaatgccca gtggttaaac gggtgtaacg tgtattcttt cactaaaccg
     7201 ccaaaacctt tcgatttcac cgtgtaaaat aaaattaacg caaatacaca aatagacata
     7261 cccagtgttg cactaatatc tgccgttggt actgcacgca aatagtgaat accaaacaga
     7321 ttggctaact gtggtaagaa atcgaccggg ataaggtcga tcgcattcat aataaacacc
     7381 caacagaaaa tcgttaatgc taatggtgca attacattgc gaggtccgtg gaagttatcc
     7441 ttcactaaac cgtttaccca ctcaataaca atctcaacaa aacattgcaa ttttcccgga
     7501 acgcccgttg ttgcattttt tgctacacgc gagaacaccc ataaaaaaat cgcacctgcc
     7561 actaaagaga agaacagtgt atctaaatgc actgcccaaa agccttcacc tgtcgataaa
     7621 aaactcaagt gatggctaat atattctgct gtcgttccag ccataacata tccttttaaa
     7681 ttaaaattag tggtgatgtt tgcttaatac aaacgggatc atattattta aaaataatgc
     7741 gataaaataa cccacgaaaa acaacacaac atctagagca gggataagtt taaaacagcc
     7801 gatcaccaaa ataatagtaa ccaaccattt cattccttca ccacgataga aagcggtcat
     7861 ttttgactga ttttttgcag atttcttaaa gaaaacccaa tatacaaaca caagatgagg
     7921 caaaaaactc gctaaagcac cgcctagaaa ggaaaaatta aaattcattt tccataaaga
     7981 cactccaagt gcaaaaacgc acaagagaaa aaattcaacc tttaatgctt gtttatattg
     8041 tgattttgct ctattgatta cagctgacac attttatcct ttttgaataa gtaaaccaca
     8101 caatcaggct aataattagc cactgttgaa tcaaagaaaa tccgtgctaa attatacttt
     8161 acaaatgaat tgttgc
//
LOCUS       DANLYI010000102         8175 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.102, whole genome shotgun sequence.
ACCESSION   DANLYI010000102 DANLYI010000000
VERSION     DANLYI010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 8175)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8175)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8175
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.102"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            78..1400
                     /locus_tag="PXD81_001597"
     CDS             78..1400
                     /locus_tag="PXD81_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009875241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter"
                     /protein_id="HDL5537611.1"
                     /translation="MSAMFIIQFAIVLLCILVGARVGGIGLGVFGGLGLAILSFGFGL
                     KPAGLPIDVMFMIMAVVSAAAAMQAAGGLDYMIKIATRILRKNPKYITFIAPLVTWTF
                     TVLAGTGHVAYSVLPVIAEVSRHNGIRPERPLSMAVIASQFAIVASPIAAAVVAVVAF
                     LEPQGVTLGNVLSVTIPATLLGLGLACVFVNKMGKELKDDPNYQRLLQDPEYVKENHI
                     TTNPTELPLKPTAKLSVGLFLFGALLVVLMGAIPALRPVFDGKPMGMAHTIEIVMLSI
                     GALIIFFCKPDGNEITKGSVFHAGMRAVIAIFGIAWLGDTLMQGHMDEVKSMVSGLVE
                     TAPWAFAFALFVLSVLVNSQGATVATLFPVAIAIGIPAPVLIGVFVAVNGYFFIPNYG
                     PIIASIDFDTTGTTRIGKYIFNHSFMLPGLLSMIFSIGFGLLLSNILL"
     gene            complement(1448..1807)
                     /locus_tag="PXD81_001598"
     CDS             complement(1448..1807)
                     /locus_tag="PXD81_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CrcB family protein"
                     /protein_id="HDL5537612.1"
                     /translation="MISIGAVLGAMCRWQLAVWLNPILSQFAFGTLAANWLGCLLIGI
                     AMGFNLGESQRLLFISGFLGSFTTFSSFSLELTEKLLAEKWGQFVTVLSLHLIGGLLL
                     TLIGVMLARAVTSGRFC"
     gene            2020..2805
                     /locus_tag="PXD81_001599"
     CDS             2020..2805
                     /locus_tag="PXD81_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HDL5537613.1"
                     /translation="MSKSLRNACIGEFIGTGFILFFGAGCVAAAQVAGANFGLWEISI
                     VWGLGVSMAIYISAGISGAHLNPAVTIALAAFYGFEKHKILPYVIAQVAGAFCSVALI
                     YFMYSDLFTAAEAAQGITRGETVGFAGVFSTYPNPNITLVTAFIVEFVITVVLMSTIL
                     AIGDDKNGLPNKALAALLIGLLIAVIGGATGPLTGFAMNPARDFGPKLFAYLAGWGEI
                     ALTGGKEIPYFIIPIVAPICGALFGAWGYKNLIHNNLPANTQE"
     gene            2809..4320
                     /gene="glpK"
                     /locus_tag="PXD81_001600"
     CDS             2809..4320
                     /gene="glpK"
                     /locus_tag="PXD81_001600"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HDL5537614.1"
                     /translation="MEKQYIIALDQGTTSSRAVLLDKNANVVEVAQREFTQIYPQAGW
                     VEHNPMEIWSSQSSTLNEVVAKAGIKPDSIAAIGITNQRETTIVWEKETGKPVYNAIV
                     WQCRRTSDITDKLKADGHEEYIRKTTGLVVDPYFSGTKVKWILDNVEGAREKAERGEL
                     LFGTVDTWLVWKLTQGRVHVTDYTNASRTMLFNIHTKQWDDKMLELLNIPRSMLPEVK
                     NSSEVYGQTNIGGQGGVRIPVAGIAGDQQAALYGHLCVKEGQAKNTYGTGCFMLMHTG
                     NTAIQSENGLLTTIACNAKGEPEYALEGSVFIAGASIQWLRDELKIVHDSFDSEYFAT
                     KEDDSNGVYVVPAFTGLGAPYWDPYARGSIFGLTRGANRNHIVRATLESIAYQTRDVL
                     EAMQSDSKAKLQALRVDGGATANNFLMQFQADILDTKVERPKVKEVTALGAAYLAGIA
                     VGFWKDLEELKDKAEIERTFTPDGDQEKRAKRYKGWKKAVRRSLEWAKEDAEE"
     gene            4472..5014
                     /locus_tag="PXD81_001601"
     CDS             4472..5014
                     /locus_tag="PXD81_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005604820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HDL5537615.1"
                     /translation="MSQFQIHTIESAPETAQEALKAVQQANGFIPNLIGVLANAPTAL
                     ETYRTVGGINSRNSLTAEEREVVQITAAVVNGCGFCVAGHTKIALKALKMPEEVVNAL
                     RATARIDDNKLDTLARFTLAVMLQKAKLTEAQLNEFFAAGYNQQNAIDVILGVSLATL
                     CNYVNNIAEIPINPELQPFA"
     gene            complement(5135..6193)
                     /locus_tag="PXD81_001602"
     CDS             complement(5135..6193)
                     /locus_tag="PXD81_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA/acyl-ACP dehydrogenase"
                     /protein_id="HDL5537616.1"
                     /translation="MSVLSSSFIQWLQQNAENLDQSNERADLLIPQIAALGLYKIGVP
                     ETLGGSGGTLVDAIKAVTELGNHSLAAAFVSWGHRTFIEHIIASKNPIPRQSYLADLL
                     NGNLTAGTGLSNAMKFLSGIEELNVNIREENGKRYLNGRLPWVTNLRSYRFVASFVAG
                     FQDERKPLVIAIPSTAKGLTRTKELEFIALQGTNTTSLVFENVELQDEWILADDAPEF
                     LAATRPAFLGLQFGLPFGLVERSLAEIEKSLPFRAILASEWQAQVDALNDIKTRLFNG
                     LNEEGYFVRNPKELFQARIDIVNVVAQTILLELQASGGRGYLKNDTSGFSRRWKEAAF
                     LPVVTPSAVQLRMVLANS"
     gene            complement(6348..7091)
                     /locus_tag="PXD81_001603"
     CDS             complement(6348..7091)
                     /locus_tag="PXD81_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL5537617.1"
                     /translation="MKQKYIYGLAGLLLLLLGWQLGTALLSQRLPMASMLAPAKTWES
                     FTWLISSGEIFPHLWASLQRIAVGLLAALVIGVPLGLLLGFSKHLEMLCTPSFQLLRM
                     ISPLSWMPIIVMLLGVGDLPIYFLLAFATVWSLIISTTSGVKSIDPQWLQLGRVMSAT
                     RKELLCKIVVPAITGHVLTGLRVAVGIAWVVLVPCEMLGVNQGLGYFILDTRDRLAYS
                     ELMAVIIIIGLIGWALDSLIRLALAKGGR"
     gene            complement(7108..8127)
                     /locus_tag="PXD81_001604"
                     /pseudo
     CDS             complement(7108..8127)
                     /locus_tag="PXD81_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250818.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
BASE COUNT         2263 a         1853 c         1784 g         2275 t
ORIGIN      
        1 cgtgccgatt aatttgatga aaaatgcaga ttttttcaaa cacagagttt catttactta
       61 tttaggagca aaaaattatg tctgcgatgt ttattatcca atttgccata gtgttactat
      121 gcattttggt tggcgcccgt gtaggcggta tcggattagg ggtgttcggt ggcttgggct
      181 tagccattct ttcatttggg tttgggctaa aacctgccgg tttaccgatt gatgtaatgt
      241 ttatgattat ggcggttgtg tcggctgcag cagccatgca agcagccggt ggtttggact
      301 atatgatcaa aattgccact cgcattttgc gtaaaaaccc gaaatacatt acctttatcg
      361 caccgcttgt aacttggaca ttcaccgtgc ttgccggtac aggtcacgtt gcttattctg
      421 tattacctgt aattgcagag gtaagccgcc acaacggtat ccgccctgag cgtccacttt
      481 caatggcagt aatcgcctca caatttgcga ttgtcgccag cccgattgct gcggcagtag
      541 ttgctgtggt tgccttcctt gaacctcaag gtgttacatt gggtaacgtg ttaagtgtga
      601 ctattcctgc gaccttattg ggcttaggtt tagcttgtgt gtttgtaaat aaaatgggca
      661 aagaattaaa agacgaccca aattatcaac gcttattgca agaccctgaa tatgtgaaag
      721 aaaatcacat tactaccaat ccaaccgaat taccgttaaa accgactgcg aaattatcag
      781 taggcttatt cctattcggt gcattattag tggtattaat gggcgcaatt ccagctcttc
      841 gccctgtgtt tgatggtaag ccgatgggga tggctcacac gattgaaatt gtgatgttaa
      901 gtattggtgc gttgattatc ttcttctgca aaccggacgg taatgaaatc accaaaggtt
      961 ctgtcttcca cgccggtatg cgtgcagtga ttgcgatttt cggtattgct tggttaggcg
     1021 ataccttaat gcaaggtcat atggacgaag tgaaatcaat ggtttcaggc ttagttgaaa
     1081 ccgcaccttg ggcatttgct ttcgcattat tcgtactttc cgtattggta aacagccaag
     1141 gggcaacggt tgcaacctta ttccctgtag cgattgctat cggcattcct gcaccggtgc
     1201 taatcggggt atttgtggcg gtaaacggct acttcttcat tccgaactac ggcccgatta
     1261 ttgcctcgat tgacttcgac accaccggca caacccgtat cggtaaatat attttcaacc
     1321 atagctttat gttaccgggc ttacttagta tgattttcag tatcggattt ggtttattac
     1381 tttccaatat cctactttaa tcgctcgcaa aacctcccgt ttgggaggtt ttttatttgc
     1441 aaaattttca gcaaaaacga ccgcttgtaa cggctcttgc aagcattaca cctatcaaag
     1501 taagcaaaag cccaccgatt aaatgtaaac tcagcactgt cacaaactgc ccccattttt
     1561 ctgctaatag tttttccgtt agttctaaag agaaactgga aaaggtggta aaactgccta
     1621 aaaaaccgct gatgaacagc aaccgctggc tctcgcctaa attaaacccc attgcaatac
     1681 caattaacaa acaaccaagc caatttgctg ctaaagtacc aaaggcgaat tggctgagta
     1741 tcgggtttag ccaaacggct aattgccagc ggcacatagc tccaagtacc gccccgattg
     1801 aaatcataag tattgaagac atcatttttc ctcatctaac gttttctcac atcataccta
     1861 aaaatgagtg atttagaaaa ataatgtgat ggatttcaca aatttgaaac ttcctatttg
     1921 cttaaaaaat gccgctttgg caatatcact ccgaaatatg ttcatattca aacatttttt
     1981 catcactttc caatcatttt catcttaagg agaaacttta tgagcaaatc cttacgtaat
     2041 gcctgtattg gcgaatttat cggtacaggt tttattcttt tctttggtgc tggttgtgtt
     2101 gctgctgctc aggtagccgg tgcgaatttc ggtttatggg aaatttccat cgtttggggg
     2161 cttggtgttt ccatggcaat ctacatttct gccggtattt ccggtgctca ccttaaccct
     2221 gcggtcacta tagcacttgc cgcattctac ggctttgaaa agcataaaat tttgccctat
     2281 gtcatcgccc aagttgccgg agcgttttgc tctgtcgcac tgatttattt tatgtatagc
     2341 gatctcttta ctgccgccga agccgctcaa ggcattactc ggggcgaaac tgttggcttt
     2401 gccggtgtgt tctccaccta tccaaaccct aatattacat tggttaccgc cttcatcgtt
     2461 gaatttgtga ttactgtggt gttgatgtcc actattttag cgattggcga tgataaaaac
     2521 ggcttgccga ataaagcctt agccgcatta ctcatcggtt tattgattgc ggtaattggt
     2581 ggagcaaccg gcccgttaac cggctttgcg atgaacccgg ctcgtgattt tggtccgaaa
     2641 ctctttgctt atcttgccgg ttggggtgaa attgctttaa caggcggaaa agaaattccg
     2701 tatttcatca ttccgattgt cgccccaatc tgcggtgcat tattcggtgc ttggggttat
     2761 aaaaacctca ttcataacaa tcttccagca aatactcagg agtaatctat ggaaaaacaa
     2821 tatatcatcg ctctcgacca aggtacaacc agctctcgtg cggttttatt agataaaaat
     2881 gccaatgtgg ttgaagtggc tcagcgtgaa tttacccaga tttatccgca agcaggctgg
     2941 gtagaacata acccgatgga aatttggtca agccaaagtt ccaccttaaa tgaagtggtg
     3001 gcaaaagccg gtattaagcc ggatagcatt gccgcaatcg ggatcacaaa ccaacgtgaa
     3061 accacgattg tgtgggaaaa agagaccggt aaacctgttt ataatgcgat tgtgtggcaa
     3121 tgtcgtcgca catcggatat taccgataaa ttaaaagcag acggacacga ggaatatatt
     3181 cgtaaaacga caggcttggt agtcgatccg tacttctccg gcactaaagt gaagtggatt
     3241 ttagataacg tagaaggggc aagagaaaaa gcagaacgtg gcgaattgct gttcggtacg
     3301 gtcgatactt ggctggtgtg gaaattaacc caagggcggg tacacgttac cgattacacc
     3361 aacgcttctc gtactatgtt gtttaacatt cacaccaaac aatgggacga taaaatgctg
     3421 gaattgctca atattccacg ctcaatgtta cctgaagtga aaaattcatc agaagtttac
     3481 gggcaaacta atatcggcgg tcaaggcggt gtgcgtattc cggtcgcggg tattgcaggt
     3541 gaccaacaag ccgctctcta cggtcattta tgcgtgaaag aaggacaagc gaagaatacc
     3601 tatggtacag gctgttttat gttgatgcac accggtaaca ctgcgattca atctgaaaac
     3661 ggtttattaa cgaccattgc gtgtaacgca aaaggcgagc cggaatatgc gttggaaggt
     3721 tcagtattta ttgccggtgc gtctattcaa tggctgcgtg atgagctgaa aattgttcac
     3781 gatagctttg acagcgaata ttttgccact aaagaagatg actctaacgg tgtttatgtt
     3841 gtacctgcct ttaccggctt aggtgctcca tattgggatc cgtatgcacg aggctcaatt
     3901 ttcggtttaa cccgtggggc aaaccgtaac cacattgtgc gtgcgaccct agagtctatc
     3961 gcttaccaaa cccgagatgt gttagaagca atgcagtctg actccaaagc gaaactacaa
     4021 gcgttacgtg tggacggtgg tgcaaccgca aacaacttct taatgcagtt ccaagccgat
     4081 attttagaca ccaaagttga gcgtccgaaa gtgaaagaag tcactgcttt aggggcagct
     4141 tatcttgcgg gtattgctgt cggcttctgg aaagatttgg aagaactgaa agacaaagcg
     4201 gaaattgaac gcaccttcac accggacggc gaccaagaaa aacgggcgaa acgctataaa
     4261 ggttggaaaa aagccgttcg ccgctcatta gaatgggcaa aagaagacgc agaagagtaa
     4321 aaacgcaacc tgtgcataaa caaagggagt ataggcaact atactccctt taagatcttt
     4381 tagttatatt aaatagtgat tttgatttgt tctaaattag ccttttcttt atgatgagcc
     4441 acaccaataa acaacctgat aaggaacatt tatgtctcaa tttcaaattc acactattga
     4501 atccgctcct gaaacggcac aagaagccct aaaagcagtg caacaagcaa acggttttat
     4561 cccaaatctg atcggcgtat tagccaacgc accaacagca ctggaaactt accgcactgt
     4621 gggtggtatt aatagtcgta acagcttaac cgctgaagaa cgtgaagtgg tacaaatcac
     4681 cgcagcagtg gtcaacggct gtggtttctg cgtggcaggt catactaaaa tcgccttaaa
     4741 agcattaaaa atgcctgaag aagtggtaaa tgctttacgt gccacggctc gcatagacga
     4801 taacaaatta gacactctgg ctcgcttcac ccttgcagta atgttacaaa aagccaaatt
     4861 aaccgaagcc caattaaacg aattttttgc cgcaggttac aaccaacaaa atgcgattga
     4921 tgtgatttta ggcgtgagct tggcaacatt atgtaactac gttaacaaca ttgccgaaat
     4981 tccaattaac cctgaattgc agccttttgc ataatagata gccgtaaaat tgaggaagca
     5041 taaaaaatgc atcctttttt atttttattg caagcggtta tatttagact attttttgca
     5101 aaataaaaga ggcaacaatt tgtctcttat ccgcttaact attcgccagc accattctca
     5161 gctgtaccgc acttggcgta acaacgggca gaaatgccgc ttctttccaa cggcggctga
     5221 agcctgaagt atcatttttc agatagccac gtccgccact cgcttgcagt tctagcagaa
     5281 tagtttgggc gacgacattg acaatgtcaa ttctcgcttg gaaaagctct ttcggattac
     5341 gcacaaaata accttcttca tttaagccat taaacagacg agttttaata tcgttgaggg
     5401 catcaacttg agcttgccac tcagaggcga gaattgcacg gaaagggagc gatttttcga
     5461 tttccgccaa actacgttca accaagccaa agggcaaacc aaattgtaaa cctaagaacg
     5521 cagggcgagt ggctgctaaa aactcaggcg cgtcatcagc caaaatccat tcatcttgta
     5581 attccacatt ctcaaatacg agagaggtag tattcgtgcc ttgcaatgcg ataaattcca
     5641 attctttggt gcgggttaag cctttggcgg tggagggaat tgcaatcact agcggtttgc
     5701 gttcgtcttg aaaaccggca acgaaactag caacaaagcg gtatgaacgt aaattggtta
     5761 cccaaggcag gcggccattt aaatagcgtt taccgttttc ttcacgaata ttcacattca
     5821 actcttcaat gccggagaga aatttcatcg cattggataa acctgttcct gcggttaaat
     5881 tgccatttag cagatccgcc aaatagcttt ggcggggaat aggatttttt gaggcgataa
     5941 tatgctcgat aaaggtacga tgtccccacg aaacaaatgc cgcagccagc gagtgattac
     6001 ccagttcggt aacggctttg atcgcatcaa ccaaagtgcc accgctgccg ccaagcgttt
     6061 ccggcacgcc gattttatat agccctagtg cggcaatttg tggaatgagt aaatccgctc
     6121 gttcgttaga ttgatctaaa ttttcggcgt tctgctgaag ccattgaata aaagaagatg
     6181 aaagaaccga cattgtgttt acctcgtata attagtttac tttgtcgcta tcttaaatcg
     6241 cttttttatg ccctagaaat aaaataaatt aaggtaatat gctttttggg aataaacaag
     6301 cggtcgattt tttgcaaaaa aatgcaattt ttcgaccgct tgtcaattta tctgccgcct
     6361 ttcgctaaag ccaagcggat taagctatcc aacgcccagc cgattaagcc gataattata
     6421 attaccgcca tcagttcgga ataggctaat cggtctcggg tgtctaaaat aaaatagccg
     6481 agtccttgat tcacgcctag catttcgcac ggtaccagca ccacccacgc aatcccaacc
     6541 gccacacgta aaccggtgag aacgtgtccg gtaatagccg gtacaacaat tttgcacagc
     6601 agttctttgc gggtagccga cattactcta cccagttgta accattgtgg atcaatgctt
     6661 tttacccctg aggtggtgga aataatcaac gaccaaacgg tggcaaacgc cagtaaaaaa
     6721 taaatcggca aatcgcctac accaagtaac atcacgataa tcggcatcca cgaaagtggc
     6781 gaaatcatac gtagtagttg gaatgacggc gtgcaaagca tttcaaggtg cttggagaag
     6841 ccgagcaata agcccagcgg tacaccaatc actaacgcag cgagtagccc gacagcaatg
     6901 cgttgcaaac tcgcccataa gtgcggaaaa atttcgccgg aggaaatcag ccaagtaaaa
     6961 ctttcccagg tttttgccgg agctaacatt gacgccatcg gcaaccgttg gcttaacaag
     7021 gctgtgccaa gctgccaacc taaaagtagc agtaataaac cggctaagcc gtaaatatat
     7081 ttctgtttca tcgttcgttc ctatcgctta tacactaatt tgctcggttc ttgtccaacc
     7141 ggtttgtaaa ttaaaggttt caacccaacc gccttgctca atcgcttttc gcacaaaggt
     7201 cggctcgtta atcgctttcg ctgcctctac cgggtcaagc tgttgtaaaa acgcattatt
     7261 gccggcaatg tgggtctgtt tgagcatttt gaccagctct tccatatagg aatcgaacgg
     7321 atacggttgg aaaccgattc gctgttggtg ccactgaggg ttttgaattg cccctgtttc
     7381 gatatattta tcccactgag cttgggttgg agccagtacc ttttccagca ctttttgatc
     7441 gtgcggcgtg tagccgctgc cccgagccaa taattgagcg gtttctgcac ggttggcaag
     7501 cgtggacact tgggcttcgg ttaagacatt caccactttt tgcacccatt ccgggcggtg
     7561 ttgaatatcg tattcgtgca ttagggttaa acagcaggcg tgatctttcc acacatcgcc
     7621 actaaaacgc agcaccttcc ccacaccttt ggcttccgct aacgcattga acggctctgc
     7681 cacaataaag ccggaaattg cctcacttgc caacgctgcc accatatctg acggagccat
     7741 tacacttaat cgcacttcac cgcttttcgg ctctttttcg gtgattttca agccattttc
     7801 tcgtaataat cgttgcaaaa cgatattatg aattgaatac caaaacggaa ttgctaccgt
     7861 tttgccggag agttcggcaa tactgttgat ttcagggcga accgttaaag ccgaacctgc
     7921 caagtgattc cacattaccg ctttcaccgg agcatttgct ccatatttcg cccacaaact
     7981 cataggcgaa agcagatgca ccacgttcac attgccactt aaaaaggctt ccaccaactg
     8041 agcccagctg cgaaacatca ccggtttttc cacctgcaca ccgtgctttt caaataagcc
     8101 tttgctgatt tttgcggtag ccaagagaaa gattcgtaat gcttaggctc ataaatcccc
     8161 ttttctagtt caatg
//
LOCUS       DANLYI010000103         8121 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.103, whole genome shotgun sequence.
ACCESSION   DANLYI010000103 DANLYI010000000
VERSION     DANLYI010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 8121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8121
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.103"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            12..284
                     /locus_tag="PXD81_001605"
     CDS             12..284
                     /locus_tag="PXD81_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005823919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HDL5537618.1"
                     /translation="MPLLFEYDPNKSQINLAKYGIDFEQAKLLWEDERKVVLEACIEP
                     ELRYFLIGKIYDKYWTAFFTPRNDVIRLISVRRSRKKEISYYEKYH"
     gene            268..510
                     /locus_tag="PXD81_001606"
     CDS             268..510
                     /locus_tag="PXD81_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HDL5537619.1"
                     /translation="MRNTISAEELERIFDEGKEDILQYCDLSTAHRPGQMKRVNVDFP
                     NWMVEALDKQARHLGVPRQALIKMWIAEKLPVQSPR"
     gene            522..1190
                     /gene="rluA"
                     /locus_tag="PXD81_001607"
     CDS             522..1190
                     /gene="rluA"
                     /locus_tag="PXD81_001607"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HDL5537620.1"
                     /translation="MALIEYNPPLEPFLDEVYRDNHILVINKPSGLLSVPGNRPEYYD
                     SAMTRVQQKYGFTEPAHRLDMATSGILLFALSKAAEKELKRQFREREPKKHYIALLWG
                     KLGEKVGDKGEMNFPLICDWENRPRQKICYERGKKAITHYEVLAHYPNNIARVKLTPI
                     TGRSHQLRVHSLALGHPIIGDKFYANPLAKSLSPRLCLHAESLTITHPITRELMTFTA
                     EPTF"
     gene            complement(1168..1743)
                     /gene="ampD"
                     /locus_tag="PXD81_001608"
     CDS             complement(1168..1743)
                     /gene="ampD"
                     /locus_tag="PXD81_001608"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HDL5537621.1"
                     /translation="MPNLKIEQGCLTGQKQVPSPHFSQRPDQEDISLLVIHYISLPPE
                     EFGGNFIDLFFQGKLEPTAHPYFEEIKDLRVSAHCLINREGEITQYVNFNDMAWHAGV
                     SCFEGREKCNEYSIGIELEGSNNQPFTQAQYQSLANLTKAIQQSYPQITDERITGHCH
                     IAPERKIDPGQYFDWAYYKHLLNEAKKSAQQ"
     gene            1803..2645
                     /locus_tag="PXD81_001609"
     CDS             1803..2645
                     /locus_tag="PXD81_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase
                     RlmJ"
                     /protein_id="HDL5537622.1"
                     /translation="MLSYRHSFHAGNHADVLKHIVQLLILRALKEKEKGFLYLDTHSG
                     VGRYSLLSFESEKTGEYLEGVARLWERDDLPEEVAFYIDELKKLNRGGKLKYYAGSPL
                     LAVQQLREQDRAILTELHPNDFPLLRQEFAKAKNVQTKRENGFQQLKSALPPKERRGF
                     VLIDPPYELKEDYELVVKAIEEGYKRFATGVYAIWYPVVLRQHTKRIVRGLMDTGIRK
                     ILQIELAVRPDSDQRGMTASGMIVINPPWQLETQMKKILPYLTEVLVPEGTGSWKVEW
                     ITPE"
     gene            2768..3409
                     /gene="pyrE"
                     /locus_tag="PXD81_001610"
     CDS             2768..3409
                     /gene="pyrE"
                     /locus_tag="PXD81_001610"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HDL5537623.1"
                     /translation="MQQYKHDFIEFALSRNVLKFGEFTLKSGRISPYFFNAGLFNTGR
                     DLAKLGEYYAKAIQASGIEYDVLFGPAYKGIPIATTVAVALANLFDVDKPCCFNRKEA
                     KDHGEGGNLIGSPLTGKILLVDDVITAGTAIRESMEIINANNAQLAGVLIALNRKEKG
                     KGELSAIQEVERDYGCQVFSIVDFDDLVAFIETSPQYAPYLPKMREYREKYGV"
     gene            3583..4672
                     /locus_tag="PXD81_001611"
                     /pseudo
     CDS             3583..4672
                     /locus_tag="PXD81_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005607068.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
     gene            complement(4737..5927)
                     /gene="metC"
                     /locus_tag="PXD81_001612"
     CDS             complement(4737..5927)
                     /gene="metC"
                     /locus_tag="PXD81_001612"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HDL5537624.1"
                     /translation="MSNYKNLSTKLVHTGRKKRNTQGAVNPVIQRASSLVFETLEEKK
                     HATQNRYKGALFYGRRGTLTHFSLQEAMCELEGGAGCYLYPCGAAAVTNAILAFVEQG
                     NHVLMTGAAYEPTQEFCNALLKRMGVSTTYYDPMIGEGLKALVQPNTKVLFLESPSSL
                     TMEVPDIPTLVRVAREINPEIVIMIDNTWSAGVLFPALEYGIDISIQAGTKYLVGHSD
                     VMIGTAVSNARCWDKLREGSYLMGQMVDADSAYITARGLRTLGIRLKQHEQSSIAVAN
                     WLAQRPEVKAVYHPALESCPGHEFFKRDFLGSSGLFSFELCEKLSNDKLANFLEHFEL
                     FSMAYSWGGFESLILANQPEEIAKIRPAIERKLTGTLIRIHVGLEAVEDLIADLEQGF
                     NRIR"
     gene            complement(5986..6525)
                     /gene="dsbB"
                     /locus_tag="PXD81_001613"
     CDS             complement(5986..6525)
                     /gene="dsbB"
                     /locus_tag="PXD81_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005614582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HDL5537625.1"
                     /translation="MLYFFKNLSQSRSAWFLLMLSCLILEGTALYFQHGMGLVPCVMC
                     IYERVALFGILFAGILGFLGNRYAIIRYLAILIWLGSAFKGLVLSIKHHDYQVNPSPW
                     NQCEFKVNFPQTLPLDQWLPQVFAPGPVNCSQSQWEMFGFSMAQWLIFTFAVYLIVAV
                     IVLISQFKRSRQRNNFMFR"
     gene            complement(6588..8120)
                     /gene="nhaB"
                     /locus_tag="PXD81_001614"
     CDS             complement(6588..8120)
                     /gene="nhaB"
                     /locus_tag="PXD81_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005614583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proton antiporter NhaB"
                     /protein_id="HDL5537626.1"
                     /translation="MDALFKSFLGKSPEWYKLCILAFLIINPLIYFFINPFVAGWFLV
                     AEFIFTLSMALKCYPLQPGGLLAIEAVVIGMTNPHHIKEEIMANFDVILLLMFMVAGI
                     YFMKQLLLYIFTKLLVAIHSKKILSLSFCLSAAFLSAFLDALTVIAVIISVGTGFYGV
                     YHKVASGNNFEDSTDITNDEKIISNKEILEQFRGFLRSLLMHAGIGSALGGVMTMVGE
                     PQNLIIASQAEWGFIEFLLRALPVSLPVLICGIATCFLVEKYKLFGYGDRLPSKVWVV
                     LARYSQLKEQKMTKQDRLKMLVQAIAGIWLIIGLALHLADVGIIGLTIIIICTAFCGI
                     TDEHTLGKAFQESMPFTALLIVFFSVVAVIIDLKLFEPIIAFVLSAEPHSQLSLFYIF
                     NGLLSMISDNVFVGTVYINEAKTALDAGIIDREQFDLIAVAINTGTNLPSVATPNGQA
                     AFLFLLTSSFAPLIKLSYGRMVYMALPYTIVLSIVGFLGLEFLLPSMTEVMINWGWFI
                     TR"
BASE COUNT         2534 a         1611 c         1753 g         2223 t
ORIGIN      
        1 tgcaatatgc tgtgccgtta ttgtttgaat acgacccgaa caaaagccaa attaatttag
       61 ccaaatatgg tattgatttt gaacaggcaa agttgctttg ggaagatgaa cgaaaagttg
      121 tactggaggc ttgcattgaa cccgaattac gctatttcct gattggcaag atttatgata
      181 aatattggac agcgtttttt acaccaagaa atgatgttat tcgtttaatt tcagttagac
      241 gttcccgtaa aaaagagatc tcatactatg agaaatacca ttagtgccga agaattagaa
      301 cgtatcttcg atgaaggcaa agaagatatt ttacaatact gcgatctttc taccgctcat
      361 cgcccggggc aaatgaaaag ggtgaatgtt gatttcccaa attggatggt agaagcgtta
      421 gataagcaag ccagacattt aggtgtgcct cgccaagcct tgattaaaat gtggattgcg
      481 gaaaaattac cggttcaatc acctaggtaa gcgagtggca aatggcatta attgaatata
      541 accctccatt agaaccattt ttagatgagg tttatcggga taaccatatt ttagtaatta
      601 ataagcctag tggtttatta tcggtgccgg gcaatcgtcc cgaatattac gacagtgcaa
      661 tgactcgagt acagcaaaaa tatggcttta ccgaaccggc tcatcgtttg gatatggcaa
      721 caagcggtat tcttttgttc gccctaagca aagcggcgga aaaagaacta aaacgccaat
      781 ttcgggaacg tgaaccgaaa aaacactata ttgcgttact ttggggcaaa ttaggtgaaa
      841 aagttgggga taagggcgaa atgaattttc cgctgatttg cgattgggaa aatcgccccc
      901 gccagaaaat ttgctatgaa cgaggcaaaa aagccattac ccattatgaa gtgttagctc
      961 attatccgaa taatatagcg agagtaaaac tcacgcctat cacggggcgt tcgcatcagc
     1021 tacgagtaca tagccttgcg ttaggtcacc cgattattgg cgataagttt tatgctaatc
     1081 cgctggcaaa aagtttatcg ccccgtttat gtttgcacgc ggagtcacta acaattactc
     1141 acccgattac aagggagttg atgacgttta ctgctgagcc gactttttag cctcgttcag
     1201 taggtgtttg taatatgccc aatcaaaata ttgccccggg tcgattttcc gttccggggc
     1261 aatatggcaa tgcccggtga tgcgttcatc ggtaatttgt ggatagcttt gctgaatggc
     1321 tttggtcaaa tttgccaaag attggtattg agcttgagta aacggctgat tattactgcc
     1381 ttccagttca atgccaatcg aatattcatt gcatttctcc cgcccttcaa aacaagagac
     1441 gccggcgtgc cacgccatat cgttaaaatt cacatattgc gtgatttccc cctccctatt
     1501 aatcaaacaa tgggcggaaa cccgtagatc tttgatttct tcaaaataag ggtgagcggt
     1561 tggttcgagt ttaccttgga aaaacagatc gataaaattt ccaccaaatt cttccggcgg
     1621 taagctgatg tagtgaataa ccaacaagga aatatcctct tgatcaggac gttggctaaa
     1681 atgcggagac ggcacttgtt tttgaccggt taaacagccc tgttcgattt ttaaatttgg
     1741 catagtctaa atagggtgaa tacgttaaaa tatggctcat tttactagaa aaagagaaga
     1801 atatgttaag ttatcgccac agtttccacg caggcaacca cgctgatgtg ctaaaacaca
     1861 ttgtccagct tttgatcttg cgagcattaa aagaaaaaga aaaaggtttt ttatatcttg
     1921 atactcactc cggtgtgggg cgttatagtc tgttaagttt tgaatcggaa aaaacaggcg
     1981 aatatttaga gggcgttgcc cgtttatggg agcgtgatga tttaccggaa gaagtggcat
     2041 tctatattga tgagctgaaa aaattaaacc gtggcggcaa gttaaagtat tacgcaggct
     2101 ccccattatt ggcggtacag caactgcgtg agcaagaccg agccattctt accgaacttc
     2161 accccaatga ttttccgctg ttacgacaag aatttgccaa agcgaaaaat gtgcaaacca
     2221 agcgagaaaa tggctttcaa caattaaaat ccgccctgcc gcctaaagag cgtcgaggtt
     2281 ttgtgttgat tgatccacct tatgaattaa aagaagatta tgaattggtg gttaaagcga
     2341 tagaagaggg ctacaaacgg tttgccactg gtgtgtatgc catttggtat ccggtggtgc
     2401 ttcgccaaca taccaaacgt atagtgcgag gtttaatgga taccggcatt cgcaaaattc
     2461 tacaaattga gcttgccgtt cgtcctgata gcgaccaacg tggaatgacg gcaagtggta
     2521 tgattgtgat caacccaccg tggcagcttg aaacccagat gaaaaaaatc ctgccatatt
     2581 taaccgaggt gcttgtgccg gaaggaacag gttcttggaa ggtggagtgg attacgcctg
     2641 agtaaattaa atacggatag cacaagcgtg gacgcttgcg ccatcataaa ttctgatttg
     2701 caatattttt tagaaaaata accgcttgtc ttatcaagaa gtgggggaaa tataaaagga
     2761 aaaaacgatg caacaataca aacacgactt tattgaattt gccttaagcc gcaatgtgct
     2821 gaaatttggc gaatttacct taaaatcagg cagaatcagc ccctattttt tcaatgctgg
     2881 gctttttaac accgggcgcg atttagccaa attaggcgaa tattacgcca aagccattca
     2941 agccagcggc attgagtatg atgtgttatt cgggccggct tataaaggca ttccgattgc
     3001 aaccacggtt gccgtggcac tagccaacct gttcgatgtg gataaacctt gttgctttaa
     3061 ccgcaaagag gcaaaagatc acggtgaagg cggtaattta atcggttctc ccttaacggg
     3121 caagattttg ctggtggacg atgtgattac cgcaggcacg gcaattcgtg agtcgatgga
     3181 aattatcaat gccaataatg cccagcttgc cggtgtgctg attgcgttaa accgtaaaga
     3241 gaaaggcaag ggcgagttgt ctgcgattca agaagtcgaa cgtgattacg gttgccaagt
     3301 tttctcgatt gtggattttg atgatttggt agcatttatt gaaacgtcgc cacaatacgc
     3361 tccttatttg ccgaaaatgc gtgaatatcg tgaaaaatac ggtgtttaac ttttgcaaaa
     3421 aaacagcaaa aactcaccgc ttgtcactct ctaatctcac cattatgaat cactttcatc
     3481 ttttggtgag attttttcaa ttaaaaagcc gataaattga ttgtgagctt tagctaattg
     3541 gcttaggata aactcctgta atacaggagt tggagaacta aaatggcaca tcaaaaatta
     3601 ccttttcacg cagacaccgt tggctcatat ttgcgttccg atgcttggaa aaaagctcac
     3661 gcagactata aagcaggcaa catttccctc gaacaacgtg atgaaattgt agaagcggaa
     3721 gtgaaaaaat tagtacaagc atcaattaga tgcgggcatt caagttgtta cagacggcga
     3781 atatcaccgc tcttggtggc atattgattt cttggaaaat ctgaacggca ttgaaggcta
     3841 tgtgccggaa aaagcctatg cgtttaaagg ggttgaggtt cgcccttata atacacgttg
     3901 ttgcggtaaa gtgtcttgga atccgaatca cccttttatt gagcatttca aaaaattcaa
     3961 tgcgattgta ggtggtcgtg cggtggcgaa atttaccatt ccaagtccaa accaattaat
     4021 gtacccgatt cagtgggatc acggcattta cccaaaccgt gaggaatttg ccaaagatgt
     4081 gcaacaggct tataaagagg cgattaaagc gttctatgat gccggctgtc gttatttgca
     4141 aattgatgat gtctattggg ggtcgttgtg taataacgtt ggtcaaccga catttgaagc
     4201 cgataaagct caagcggtgg cgaatattca agccattctt gccgataagc cggaagattt
     4261 aaccattacc acccacgttt gccgaggcaa ctataaatct tcttatttat tgacaggggc
     4321 ttatgatccg attgccgacg gcttatttgc tcaaaccaac tatgatgggt atttcttaga
     4381 atatgatgac gagcgttcag gcggttttga gccgttaaaa cattttgcca acaacaaagg
     4441 gcgtgtggta ttgggcttaa ttagttcaaa attccctgaa ttggaagata aggaagcggt
     4501 taaagcccgt attcaagaag cggcaaaata tgtgccgtta gagcagcttt gcctaagccc
     4561 gcaatgtggt tttgcctcaa ccgaagaggg caatattatg tcggaagcac agcaatgggc
     4621 gaaagttcgt cttgtggagg aaatcgccca agaagtttgg ggcgaagatt aattagagtg
     4681 aaaaatgccc gtaacaagcg ggcatttttt gtgaattttt tgcaaattca agccatttag
     4741 cgaatgcggt taaaaccttg ttctaaatcg gcaatcaaat cttccaccgc ttccaagcca
     4801 acgtgaatgc gaattaatgt tcccgtcagt ttgcgttcaa tcgccgggcg gattttggca
     4861 atttcttccg gctgattggc aagaatcagc gattcaaacc caccccaaga atacgccatt
     4921 gaaaacagtt caaaatgctc taggaagttg gctaatttgt cattcgagag cttttcgcaa
     4981 agttcaaatg aaaatagacc gcttgaacct aaaaaatccc gcttaaaaaa ttcgtgcccc
     5041 gggcagcttt ccaacgccgg gtgataaact gctttcactt cagggcgttg agccagccag
     5101 ttagctaccg caatgctact ttgttcgtgc tgttttaagc gaatgcctaa ggtgcgtagc
     5161 ccccgagcgg tgatgtaagc ggaatcagca tccaccattt gccccattaa ataagagcct
     5221 tcacgcagtt tatcccaaca acgggcgtta gaaaccgcag taccgatcat cacatcagaa
     5281 tgcccgacta aatatttagt gccggcttga atggaaatat caatgccata ttctaaagca
     5341 gggaacagca caccggcaga ccaagtgttg tcgatcataa tcacgatttc tgggttgatt
     5401 tcacgagcaa cacgcacgag agtcggaata tcaggcactt ccattgttag cgagctcggc
     5461 gattctaaaa aaagcacttt ggtgttcggt tgcacaaggg ctttcaagcc ttcaccaatc
     5521 atcgggtcat aataagtagt tgaaacaccc atacgtttta acagcgcatt acagaattct
     5581 tgtgtcggtt cgtaagctgc gcctgtcatt aaaacgtggt tgccttgttc cacaaaagct
     5641 aaaatcgcat tggtgactgc cgccgcaccg caagggtaaa ggtagcaacc tgctccacct
     5701 tctaattcac acatcgcttc ttgtaatgaa aaatgagtga gagtgccacg tctgccataa
     5761 aataatgctc ctttgtagcg gttttgtgta gcgtgttttt tctcttctaa ggtttcaaac
     5821 actaaagatg aagcacgctg aatcaccggg ttaactgctc cttgtgtgtt acgtttttta
     5881 cgccctgtat gaacaagttt ggtggaaaga tttttatagt tagacataac ttttcctaaa
     5941 gtaaaatgca taggggcaaa tcaacatttg cccctttatt aataattaac gaaacataaa
     6001 gttatttctt tgacggctac gtttaaattg gctgattagc acaatgaccg caacaattaa
     6061 gtaaaccgca aaagtaaaga ttagccattg tgccattgaa aagccgaaca tttcccattg
     6121 gctttggcta caattaaccg gaccgggggc aaacacttgt ggtagccatt ggtcaagcgg
     6181 caaggtttgc gggaaattaa ctttaaattc gcattgattc catggtgatg gattgacctg
     6241 ataatcgtgg tgtttgattg aaagtactag ccctttaaat gcgctaccaa gccaaattag
     6301 aattgccaag tagcgaataa tcgcatatct attaccaaga aatcctaaaa taccggcaaa
     6361 taaaatacca aataaagcaa ctcgctcata aatacacatt acacaaggta ctaatcccat
     6421 cccatgttgg aaataaagcg ctgtgccttc aagtataaga cagcttaaca ttagtaaaaa
     6481 ccatgcactg cgagattgtg atagattttt aaaaaaatag agcatagtta ttcctcattt
     6541 tgtgaactta aggcattcta ccttatttgg tatgagttat ctataaacta acgtgtaata
     6601 aaccatcccc aatttatcat tacttcagtc atagaaggta gtaagaattc taaccctaag
     6661 aaaccgacaa tagatagaac aatggtataa ggtaatgcca tatagaccat tctgccgtaa
     6721 gacagtttaa ttaacggcgc aaaggaggac gttaataaga ataagaaagc agcttgtccg
     6781 tttggggtag caacagaagg aagatttgtg ccagtattaa ttgcaactgc aatcaggtcg
     6841 aactgctcac gatctataat gccggcatct agtgcggttt tagcttcatt aatgtaaaca
     6901 gtaccgacga agacgttatc tgaaatcatc gagagcaaac cattgaaaat atagaataat
     6961 gaaagctgtg aatgtggttc ggcagataac acaaaggcaa tgatcggctc aaataatttt
     7021 aaatcaataa tcacggcaac aaccgagaaa aacacaataa gcaatgcagt aaaaggcata
     7081 gattcttgga atgctttccc taaagtgtgt tcatcagtaa tgccacaaaa tgcagtgcaa
     7141 ataatgatga tggttaagcc aataatccct acatcggcaa gatgtagcgc taagccaata
     7201 attaaccaaa ttccagcaat ggcttgcacc agcatcttta agcgatcttg tttcgtcatt
     7261 ttttgttctt ttagttggct atagcgagcg agtacgaccc aaactttgct aggaagccgg
     7321 tctccataac caaataattt atatttttct acaagaaagc aggtcgcaat cccacaaatt
     7381 aacacgggta agctaactgg taatgctcta agtaagaatt cgataaatcc ccattccgct
     7441 tgggaggcaa taattaagtt ttgtggttct cccaccatgg tcattacgcc acctaaagct
     7501 gagccaatac cggcgtgcat caataaactt cttaaaaaac cacgaaattg ctctaaaatt
     7561 tctttattag agatgatttt ttcatcattg gtaatatcag tagaatcttc aaagttatta
     7621 cctgatgcga ctttatgata tacgccgtag aaacctgttc caacgctaat gattacagca
     7681 ataaccgtta aagcatcaag aaaggcggat aaaaaagcag cgcttaagca gaaagaaagg
     7741 gaaagaattt ttttcgaatg aatggcgaca agcagtttgg tgaaaatata aagcaataat
     7801 tgcttcataa agtaaatacc ggcaaccata aacattaaaa gaagaataac atcaaagttt
     7861 gccataattt cttctttaat gtggtgagga tttgtcatac cgattacaac tgcttcaatc
     7921 gctaataaac cacctggctg taatggataa cattttaatg ccatagagag agtaaaaata
     7981 aattcagcga ctagaaacca gcctgcaaca aaaggattga taaagaaata aatgagagga
     8041 ttgataatta aaaatgccaa aatgcacaat ttataccact cggggctttt tcctaagaaa
     8101 cttttgaaca gagcatcaat g
//
LOCUS       DANLYI010000104         7963 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.104, whole genome shotgun sequence.
ACCESSION   DANLYI010000104 DANLYI010000000
VERSION     DANLYI010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 7963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7963
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.104"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            45..980
                     /gene="pheS"
                     /locus_tag="PXD81_001615"
     CDS             45..980
                     /gene="pheS"
                     /locus_tag="PXD81_001615"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HDL5537627.1"
                     /translation="MDQNLEALEEFRVEYFGKKGHFTTLMQELRNIAAEERPAIGAKI
                     NEAKQVVLDVLNAKKEEWEQAVLNAKLANESIDVSLPGRKTELGGLHPVSITIERVVK
                     FFSELGFTVANGPEIETDYYNFDALNIPDHHPARADHDTFWFDANRLLRTQTSGVQIR
                     TMENVKPPIRIVAPGRVYRNDYDQTHTPMFHQIELLYVDKKANFTELKGLIHDFLKAF
                     FEEDLQVRFRPSFFPFTEPSAEVDVMRQNGKWLEVLGCGMVHPNVLRNVGIDPEEYSG
                     FAVGMGVERLTMLRYNVTDLRSFFENDLRFLKQFK"
     gene            1028..3415
                     /gene="pheT"
                     /locus_tag="PXD81_001616"
     CDS             1028..3415
                     /gene="pheT"
                     /locus_tag="PXD81_001616"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012262881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HDL5537628.1"
                     /translation="MKFNESWLREWVNPAISTEQLCDQITMLGLEVDDVEPVAGAFSG
                     VVVGEVVECAQHPDADKLRVTKVNVGGERLLDIVCGAPNCRQGLKVACAVEGAVLPGD
                     FKIKKTKLRGQPSEGMLCSYSELGIKEDHSGIIELPADAPIGQDFREYLNLNDVAIEI
                     SLTPNRADCLSIAGIAREVGVINRVDVKAPTITDVKATISDKVSVELQAPEACPRYLA
                     RVVKNVNVKATSPLWLQEKLRRCGIRSIDPIVDITNLSLLELGQPMHAFDAAKIEGGI
                     QVRMAKEGEELVLLDGTNAKLQSNTLVIADSKGALAMAGIFGGEASGVSENTKDVVLE
                     SAFFAPLAITGRARQYGLHTDASHRFERGVDPELTRQAMERATALLLEICGGEAGEIV
                     EAVSQENLPKRNTVTLRRSKLDSVIGHHIDDATVTDILTRLGLNVTFADNSWTAIAPS
                     WRFDIEIEEDLIEEIARIYGYNSIPNNSPLAHLTMKGTPEKLLEANRIRMALVDSDYQ
                     EVITYSFVDPKKQSLLHPQQEALILPNPISSEMSAMRVSLLTGLLDTIVYNQSRQQNR
                     VRIFEAGLRFIPDSNAESGVRQEFVFGAAIVGDKRPVHWENKSEAADFFDLKGDIERI
                     LSLTSIRHDLQFVAKQFPALHPGQSAAIMLDGKEIGFIGTVHPSIVQKFGIKGKPIVC
                     EILASAIAERPVTQAGEISKFPANNRDIAVVVDESVPAGEVLEACRKAGGNKLVAVNL
                     FDVYRGSNLESGKKSLAISLTVQDNEKTLEENEINAVIQAVLAELATRFNAVLRD"
     gene            3473..3769
                     /locus_tag="PXD81_001617"
     CDS             3473..3769
                     /locus_tag="PXD81_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005710633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HDL5537629.1"
                     /translation="MALTKIEIAENLIEKFGLEKRVAKQFVELFFEEIRSSLENGEEV
                     KLSGFGNFSLREKKARPGRNPKTGENVAVSARRVVVFKAGQKLRERVEQASSQS"
     gene            3808..4329
                     /locus_tag="PXD81_001618"
     CDS             3808..4329
                     /locus_tag="PXD81_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HDL5537630.1"
                     /translation="MKLTKRWLSLGCLLAITAMLVACSSSDTTSKSQVKSKVYRSIYQ
                     SDLSDPIMAISRLSEHQHEWKGTRYRLGGNSKAGIDCSGFMQVTFKDLFGIDLPRTTT
                     EQAKAGERISKDELRTGDLVFFKTGRGPNGKHVGVYVKNGQFLHASTKGGVIYSDLDS
                     PYWSKAFWQARRL"
     gene            4345..5388
                     /gene="degS"
                     /locus_tag="PXD81_001619"
     CDS             4345..5388
                     /gene="degS"
                     /locus_tag="PXD81_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HDL5537631.1"
                     /translation="MLKKIGQAVIFGLFCAILILFVAPIIQSKGSSFSLSAFTQKEIV
                     SYNAAVKIASPAVVNVYSRNFSSENETFEVKNLGSGVIMSSDGYILTNRHVIEDADQI
                     VIALQNGAISTNVKLVGDDSLTDLAVLKIEGENLPTIPQNPERKLQIGDVVLAIGNPL
                     NLGQSITQGIVSAIGRKTLTESGRQNFIQTDVSINQGNSGGALINTAGELIGINTLRF
                     GKNTNELSEGLNFAIPINLANQIMSKIIKDGRVIRGYFGVSTDLLDTAKMQGYEKGVI
                     IVNVDKNGPAAKAGIQAGDLVLKVGDIEAFSPSQMMEELAEMKPYTTVKVLVQRDEQL
                     LNFDVTITELQTQ"
     gene            5397..6071
                     /gene="cmk"
                     /locus_tag="PXD81_001620"
     CDS             5397..6071
                     /gene="cmk"
                     /locus_tag="PXD81_001620"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HDL5537632.1"
                     /translation="MKKIVITVDGPSGAGKGTLCHALAEKLGFDFLDSGALYRITALS
                     AIKKEVPLDDEEKLAEVGRNLDIQFLPENGEIQVILEGENVGDLIRTAEAGQNASKVA
                     AFPLVREALLERQRAFSSEKGLIADGRDMGTVVFPDAQIKLFLDASAEERTKRRVKQL
                     QEKGFNANFDEILAEIKERDFRDRNRSVAPLVPAKDALLLDSTHLSIEDVINQALNHI
                     AQYIKF"
     gene            6163..7830
                     /gene="rpsA"
                     /locus_tag="PXD81_001621"
     CDS             6163..7830
                     /gene="rpsA"
                     /locus_tag="PXD81_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006717605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HDL5537633.1"
                     /translation="MSELSFAQLLEESFAATPRLGDVVKGTVVAIQKGLVIVDTGFKS
                     ESAIDASEFTNAQGELEVQVGDEVDVVLKAVEDGFGATVVSRGDAKRNEAWIALEKAF
                     EEQATVIGFVNGKVKGGFTVELNGVRAFLPGSLVDTRPVRDSLNLEGKEYEFKVIKLD
                     QKRNNVVVSRRAVIETESNQDRDAVLANLVEGSEVKGTVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEVVNVGDEVTVKVLKFDKDKTRVSLGLKQLGQDPWAVIAENHPV
                     NSKLTGKVTNLTDYGCFVEILDGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLE
                     VDEERRRISLGLKQCKANPWEQFAATHSKNDKVSGKIKSITDFGIFIGLEGGIDGLVH
                     LSDISWNVSGEEAVRNYKKGDEVEAVVLQVDAVKERISLGIKQLESDPFTNFVDGTKK
                     GAILTGKVVEVDAKGVKVELEGGVEAFIRANEATAERVEDITSVISVGDSIEAKYTGV
                     DRKSRVVNLSVRAKNEAEESAAIAQVNKEEVVVPNAFAEAFKAAKGE"
BASE COUNT         2499 a         1296 c         1846 g         2322 t
ORIGIN      
        1 acctaaaaga tttaactaat aaagcacgtg aagctttaga taagattgat caaaatttag
       61 aggctttaga agagtttaga gtcgaatatt ttggtaaaaa agggcatttt acaacgttaa
      121 tgcaagaatt acgcaatatt gcagcggaag aaagaccggc aatcggagca aaaattaatg
      181 aagcaaagca agttgtgctt gatgtgttaa atgccaaaaa agaagaatgg gaacaagctg
      241 tattaaatgc aaaattagct aatgagagca ttgatgtttc actgccgggg cgtaaaactg
      301 aattaggtgg tttgcaccct gtttctatca ctattgagcg tgtggtgaaa tttttctctg
      361 aattaggttt taccgtagca aacggacctg aaattgaaac cgattactat aacttcgatg
      421 cgttaaacat tccggatcat catccggctc gagcggatca cgatacgttt tggtttgatg
      481 ccaatcgttt attaagaacc caaacctcgg gtgtgcaaat ccgtacaatg gaaaatgtaa
      541 aaccgcctat ccgtattgtg gcaccggggc gtgtatatcg taatgactac gaccaaaccc
      601 acacaccgat gtttcaccaa atcgaattgt tatatgtgga taaaaaagca aactttaccg
      661 agttaaaagg cttaattcac gattttctca aagcattctt tgaagaagat ttacaagtgc
      721 gtttccgccc gtcattcttc ccattcactg agccttctgc agaagtagat gtaatgcgtc
      781 aaaacggcaa atggttagaa gtgttaggtt gcggtatggt acacccgaat gtgttacgca
      841 atgtagggat tgaccctgaa gaatatagcg gttttgctgt gggaatgggc gtcgagcgtt
      901 taacgatgtt acgctacaat gtcacggatt tacgttcatt ctttgaaaat gacttacgtt
      961 tcttaaaaca atttaagtaa tacaagcggt taaaaattta gaaaatttta caaataggat
     1021 agaaataatg aaattcaatg aatcttggtt gcgtgaatgg gtaaacccag caattagcac
     1081 agaacagtta tgcgaccaaa ttacaatgtt agggttagaa gttgacgatg tagagccggt
     1141 agctggtgcg ttttcgggtg tagttgtggg tgaagtggtg gaatgcgccc agcacccgga
     1201 tgctgacaaa ttgcgtgtca ctaaagtgaa tgttggtggc gaacgtttat tagatattgt
     1261 ttgtggtgca ccaaactgcc gtcaaggttt aaaagtggct tgcgctgttg aaggtgcagt
     1321 gctaccgggc gatttcaaaa ttaagaaaac caaattgcgt ggtcagcctt cagaggggat
     1381 gttatgttct tactcagaac ttgggattaa agaagatcat agcggtatta ttgaattacc
     1441 tgctgatgca cctattggtc aagatttccg tgaatattta aatttaaatg atgtggcgat
     1501 tgaaatcagc cttacgccaa accgtgccga ctgcttaagc attgccggca ttgctcgtga
     1561 agtgggggtg attaaccgtg tcgatgtgaa agctccaact attacagatg taaaagccac
     1621 gatttcagat aaagtttcag tggaattaca agcaccggag gcttgccctc gctatttagc
     1681 ccgtgtggtg aaaaatgtga atgtcaaagc gacatcgcca ctttggttac aagaaaaatt
     1741 acgccgttgt gggattcgct caatcgatcc aatcgttgat attactaact tgagcctttt
     1801 agaattaggg cagccaatgc acgcctttga tgcagcgaaa attgaaggtg gcattcaagt
     1861 gcgtatggca aaagaaggcg aagaattggt attgctagat ggcacaaacg ctaaattaca
     1921 atcgaatacc ttagtgattg ctgatagcaa aggggcattg gctatggcag gtattttcgg
     1981 tggcgaagcc agcggggtga gcgagaatac gaaagatgtg gtgttagagt ctgcattttt
     2041 tgctccatta gcgattaccg gtcgcgctcg tcaatatggg ttacataccg acgcatcaca
     2101 ccgttttgag cgtggggttg atccagaatt aactcgtcaa gcaatggagc gtgcaacggc
     2161 attattactg gaaatttgcg gcggtgaagc aggtgaaatt gtggaagccg tgagccaaga
     2221 aaacctacca aaacgcaata ctgtcacgct tagacgcagt aaattagatt cggtgattgg
     2281 tcatcatatt gacgatgcaa cggtaaccga tattttaacc cgcttaggct tgaacgtaac
     2341 atttgcggat aatagttgga cggcaattgc tccaagttgg cgttttgata ttgaaattga
     2401 agaagattta attgaagaaa tcgcgcgaat ttatggctac aacagtatcc caaataattc
     2461 gccacttgct catttaacga tgaaaggtac gccggaaaaa ttactggaag caaaccgtat
     2521 tcgtatggca ttggtagata gtgattacca agaagtgatt acttatagct ttgttgatcc
     2581 gaaaaaacaa tcattattgc acccacagca agaagccttg attttaccaa acccgatttc
     2641 aagtgaaatg tcggcaatgc gtgtttctct tttaaccggt ttgttggaca ctatcgtgta
     2701 taaccaaagc cgtcagcaaa accgtgtgcg tatttttgaa gcaggtctac gttttattcc
     2761 ggatagcaat gcggaatcag gcgtgcgtca agaatttgtt tttggtgcgg cgattgtagg
     2821 cgataaacgt ccggtacatt gggaaaacaa atcagaagca gccgatttct ttgatttaaa
     2881 aggcgatatt gagcgtattc tttctttaac ctctatccgc cacgatctgc aatttgtcgc
     2941 aaaacagttc ccagcattac acccggggca atcggctgcg attatgcttg atggcaaaga
     3001 aattggtttt atcggaactg ttcacccgtc aattgtgcaa aagtttggca ttaaaggcaa
     3061 accgattgta tgtgaaattc tagcatcagc tattgcagag cgtccggtta cacaagcggg
     3121 cgaaatttct aaatttcctg caaataaccg tgatattgcg gtagtggttg atgaatctgt
     3181 tcctgcaggt gaggtgttag aggcttgtcg taaagctggt ggtaataaat tagtggcagt
     3241 aaatttattt gacgtttacc gtggttcgaa tttagaaagt ggtaagaaga gtttagccat
     3301 tagcttaacg gttcaagata atgaaaaaac attagaagaa aatgaaatca atgcggtaat
     3361 tcaagcggta ttagctgaat tagcaacacg tttcaatgcg gtacttcgtg attaatcagt
     3421 aaaatatggc tgatttattt tatcagccat aacaataaac taaggataaa tgatggcgct
     3481 taccaagatt gaaattgcag aaaacctgat tgaaaaattt gggttagaaa agcgtgtagc
     3541 gaagcaattt gttgaattgt tctttgaaga gatccgatcc tccctcgaaa atggtgaaga
     3601 agtgaaatta tctggttttg gtaatttttc actgagagaa aaaaaagctc gtccaggaag
     3661 aaatcctaaa acgggggaaa atgtggctgt ttcagcccgt cgagtagtag tttttaaagc
     3721 tgggcaaaaa ttacgagaac gtgttgagca agctagctcc caatcatagg ggcagttata
     3781 ttagttagat ttaatgtgga gtaataaatg aaattaacca aacggtggct ttccttaggt
     3841 tgtttgttag ctattacagc gatgttagtg gcttgttcaa gttcagatac aacctctaag
     3901 tctcaagtaa aaagtaaagt ttatcgttca atataccaat cagacctttc tgatcctatt
     3961 atggcaatta gtcgattaag tgaacatcaa cacgaatgga aaggcactcg ctatcgttta
     4021 ggtggcaata gcaaggctgg catcgactgt tctggtttta tgcaagtcac ttttaaagat
     4081 ctttttggga tagatttgcc gagaaccacc acagagcagg ctaaggctgg tgagcgtata
     4141 tccaaagacg aattgcgtac aggtgattta gtttttttca agacagggcg tggtccaaat
     4201 gggaaacacg ttggtgttta tgtaaaaaat gggcaatttc ttcacgcctc aacaaaggga
     4261 ggggtgattt attctgattt ggattcgcca tattggtcta aggctttctg gcaggctcgt
     4321 cgtttataat ttagggtaac atacatgctg aaaaaaatcg gacaagcggt catatttgga
     4381 cttttttgtg caattttaat tttgtttgta gctccaatta ttcagagtaa agggagttcg
     4441 ttttcattat cagcattcac tcaaaaagag attgtaagct acaatgcagc ggttaaaata
     4501 gcctcccccg cagtcgttaa cgtatatagc cgaaatttta gttctgaaaa tgaaacattt
     4561 gaagttaaga atttaggttc gggtgtgatt atgagtagcg atggctatat tttaactaac
     4621 aggcatgtga ttgaagatgc ggatcaaatc gtgattgctt tgcaaaacgg tgctatttcg
     4681 actaatgtaa aattagtcgg cgatgattct ttaactgatt tagctgtttt aaaaattgag
     4741 ggtgagaatt tacctactat tccacaaaat ccggagcgca aactacaaat tggcgatgtg
     4801 gtgttggcaa ttggtaatcc tttgaattta ggacagagca ttacgcaagg gatagtcagt
     4861 gctattgggc gaaaaacctt aacagaaagt ggtagacaaa attttattca gacagatgtt
     4921 tccattaatc aagggaactc aggtggtgct ttaattaata ctgctggtga gctaataggt
     4981 attaacactt tgcgttttgg gaaaaatacg aatgaattgt cagagggcct aaattttgcg
     5041 attcccatta atctggcgaa tcaaattatg agtaagatca ttaaagatgg ccgagttatt
     5101 cgtggctatt ttggggtaag tacagatttg cttgacaccg caaaaatgca aggttatgaa
     5161 aaaggggtga tcattgttaa tgtagataaa aatgggccag ccgcaaaagc ggggattcaa
     5221 gcaggtgact tagtgcttaa agtcggagat attgaggcat tctctccttc tcaaatgatg
     5281 gaagagcttg ctgaaatgaa accttatact acagtaaaag tattggttca acgtgatgaa
     5341 caattactta actttgatgt aactattact gagttacaga ctcaataggg tagaaaatga
     5401 aaaaaatagt gattacagtt gatgggccga gtggtgcagg taaaggaacg ttatgtcatg
     5461 cattggctga aaaattaggg tttgattttt tagattctgg agccttatac cggattactg
     5521 cattaagcgc aattaaaaaa gaggttcccc ttgatgatga ggaaaaacta gcggaagttg
     5581 ggcgtaatct tgatattcaa tttttgccag aaaatggcga gattcaggtg attttggaag
     5641 gagaaaatgt aggcgactta atccgaacgg cagaagcagg tcaaaatgcc tctaaagtcg
     5701 ctgctttccc tttggtacgt gaagcacttt tagaacgcca aagagccttt agtagcgaaa
     5761 aaggtctgat tgcagatgga cgagatatgg ggacagttgt gtttcctgat gcacaaatta
     5821 agctgttttt agatgcaagt gccgaagaac gcacaaaaag acgtgtaaaa cagttgcaag
     5881 aaaagggatt taatgctaac tttgacgaga ttttagccga gataaaagaa cgtgattttc
     5941 gcgatagaaa tcgatcagtc gcgccacttg ttccggctaa agatgcgttg ctgttagatt
     6001 caacgcattt atcaattgaa gatgtgatta accaagcatt aaatcatatc gctcaataca
     6061 ttaaatttta gtttgttgct tcacatagga tttgtgtagc ttttattatc aaccccgttt
     6121 aacaggacgt tagatggatg tttattttta agaagattta ttatgtcaga attatcgttt
     6181 gctcaactac ttgaagaatc atttgcagct acaccacgtt taggtgatgt tgttaaaggt
     6241 actgttgttg ctatccaaaa aggcttagtt attgttgata caggtttcaa atctgaatct
     6301 gcaatcgatg cctcagaatt tactaacgca caaggtgaat tagaagttca agtgggtgat
     6361 gaagtagatg ttgttcttaa agctgtagaa gacggttttg gtgcaacagt agtttctcgt
     6421 ggtgatgcaa aacgtaacga agcttggatt gctttagaga aagcatttga agaacaagct
     6481 acagttatcg gtttcgtaaa tggtaaagta aaaggcggtt tcactgttga attaaacggt
     6541 gttcgtgcat tcttacctgg ttcattagtt gatactcgtc cggttcgtga ttctttaaac
     6601 ctagaaggta aagagtacga attcaaagta atcaaattag atcaaaaacg taacaacgta
     6661 gtagtatctc gtcgtgctgt tatcgaaaca gaaagcaacc aagatcgtga tgcggtatta
     6721 gcaaacttag ttgaaggttc tgaagttaaa ggtacagtta aaaacttaac tgactacggt
     6781 gcattcgtag atttaggtgg cgttgacggt ttattacata tcacagatat ggcttggaaa
     6841 cgtgttaaac acccaagtga agtggttaat gtaggcgatg aagttactgt taaagtatta
     6901 aaatttgaca aagacaaaac tcgtgtatca ttaggcttaa aacaattagg tcaagatcct
     6961 tgggctgtaa ttgctgaaaa ccacccggta aacagcaaat taactggtaa agtaactaac
     7021 ttaactgact acggttgctt cgttgagatt ttagacggtg ttgaaggttt agttcacgtt
     7081 tcagaaatgg attggacaaa caaaaacatt cacccatcta aagtggttaa cgtaggcgat
     7141 gtagttgaag taatggtact tgaagttgat gaagaacgtc gtcgtatctc attaggttta
     7201 aaacaatgca aagcaaaccc ttgggaacaa tttgcggcaa ctcacagcaa aaatgataaa
     7261 gtatcaggta aaatcaaatc aattactgac ttcggtattt tcatcggttt agaaggtggt
     7321 attgacggtt tagttcactt atctgacatt tcttggaatg tttcaggtga agaagcagtt
     7381 cgtaactaca aaaaaggtga tgaagtagaa gctgttgttc tacaagttga tgcagtgaaa
     7441 gagcgtatct ctttaggtat caaacaatta gaatctgatc ctttcacaaa ctttgtagat
     7501 ggtactaaaa aaggtgcaat cttaacaggt aaagttgtag aagttgatgc taaaggcgtt
     7561 aaagttgaat tggaaggtgg cgttgaagca tttattcgtg caaacgaagc aactgctgag
     7621 cgtgtagaag acatcacttc tgttatttct gttggcgatt caatcgaagc gaaatacacc
     7681 ggtgttgatc gtaaatctcg cgtagttaac ttatctgttc gtgcaaaaaa cgaagctgaa
     7741 gaatcagcag caattgcaca agtgaacaaa gaggaagttg ttgtacctaa tgcatttgct
     7801 gaagctttca aagcagctaa aggcgaataa ttagtataga ctaatctaaa atgggtatgt
     7861 tttgaacata cccatttaaa cttagaaatg ccattttgaa tacttatttt cataataaat
     7921 gattattcaa aatagaatta atttttggta gaaataatat gac
//
LOCUS       DANLYI010000105         7953 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.105, whole genome shotgun sequence.
ACCESSION   DANLYI010000105 DANLYI010000000
VERSION     DANLYI010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 7953)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7953
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.105"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            complement(<1..265)
                     /locus_tag="PXD81_001622"
     CDS             complement(<1..265)
                     /locus_tag="PXD81_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HDL5537634.1"
                     /translation="MPFYAGQVKCIFIDPPYNTKSAFTHYDDNLEHSIWLSMMYPRLE
                     ILRELLAEDGSIWITLDDNESHYLKIMLDEIFGRKNFISNLIWE"
     gene            complement(304..528)
                     /locus_tag="PXD81_001623"
     CDS             complement(304..528)
                     /locus_tag="PXD81_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase N-4/N-6"
                     /protein_id="HDL5537635.1"
                     /translation="MTTLTWYGKEEAVKKAKQTPYCLLRELPDFSHNIARQPTADSRQ
                     PTADSRQPTADSRQPTAQLLLKPYLKSSKI"
     gene            complement(669..3155)
                     /gene="torA"
                     /locus_tag="PXD81_001624"
     CDS             complement(669..3155)
                     /gene="torA"
                     /locus_tag="PXD81_001624"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
                     /protein_id="HDL5537636.1"
                     /translation="MKKQDNIDVSRRGFLKNSSLSLAAGAGLASGTTGVVGALASTSA
                     KAAEMKTVVTAAHWGPLGVVVENGKVVKSDSVFEISVPNELQHVVADQVHGETRVKYP
                     MVRKGYLEGNKDTTLRGRDEWVRVSWDKAFELVANEMKRVREAHGANAIFGGSYGWYS
                     SGALHASRTLLQRYLNVTGGFVGVKGDYSTGAAQVIMPHVLGTIEVYEQQTSWEVILE
                     SSDIVVLWSANPLNTLRIAWTSTDQQGIEYFKKLKESGKRIICIDPVKSESCEFLGAE
                     WVPVNTGTDVALMLGMAHTLVAEGKHDQAFLKKYTTGFEKFEEYLLGKTDNQPKDAKW
                     ASQICGVPEETIKKLATDFQSKRTMLMAGWGIQRQRHGEQGHWMLVTLAAMLGQIGLP
                     GGGFGLSYHYSNGGVPSATGGILGSITANPAVEAGAKTWLDETSKSSFPVARISDALL
                     NPGKTIDYNGTQITYPDIKIVYWAGGNPFTHHQDTNTLVKAWQKPDTIIVNECHWTPT
                     ARMSDIVLPATTSYERNDLTMSGDYSMMNIFPMKQVVEPQFEAKNDYDIFAELAKRAG
                     KEAEFTEGKSEMDWLQSFYQTAFEAARKNRVIMPKFEKFWQENKPLSFKASDKAKKWV
                     RYEEFRNDPLLNPLGTPSGKIEIFSEVIAKMNYDDCKGHPTWMEHEEYSGKTTAEEPL
                     ALVTPHSKWRLHSQLAYSSLRKQYAVNDREPVLIHTEDAAARGITSGDIVRIFNKRGQ
                     VLAGAVVTDGIIKGTLALHEGAWYDPLDLGTSEQPLCKNGNPNVLTRDEGTSKLAQGN
                     SPNTATVQVEKFTGQAPEVTVFKEPKYTQG"
     regulatory      complement(3174..3370)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            complement(3374..4489)
                     /locus_tag="PXD81_001625"
     CDS             complement(3374..4489)
                     /locus_tag="PXD81_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HDL5537637.1"
                     /translation="MAKMKKIAVFVGIMALGAAVLGGTQYAMKATSSTEFCVSCHSMT
                     HPQAEWEGSVHFSNRKGIRAECSDCHLPQDGLHYVKAKIIALKDVWHTVVTNKLPDQE
                     AYEAHRLEMAQRVWAEMKENDSMTCKSCHSFDAMVLSEQTEAAQKMHKLAQETNQTCI
                     DCHKGIAHFMPDVPVDNAAAMGELAKHGAEFSTNDKTLYTLAMTPVQLAQGGEIRLLP
                     YAELHQWTAEGEQVKATVNGWQQEGAESVVYMDLGKRIMVALLPDDAQSKVNVTKTVF
                     DNVTNSNWKEINFEITAPKNAVTANITALNHFGDNLNQTHCSGCHAPIGAEHYTANQW
                     IGVVNSMKDRTSMSADDVRAVTIYLQRNAKDVAGSSH"
     gene            complement(4631..6382)
                     /locus_tag="PXD81_001626"
     CDS             complement(4631..6382)
                     /locus_tag="PXD81_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL5537638.1"
                     /translation="MDKRSRWSCLVNEQGEISEWEDKAGADYFAELPQICHYAQALLY
                     SDKNSLQFYYSSNHQWLKISLERHQSFVLTQAEHTDLPYGLSKREIELLTLISSGLSN
                     EDIANELCISKRTVDKHIENIFAKTHTKSRTLLAVFAITEHLICLPTPGKLTQSILAS
                     FNIEQLALQIIAKKQPLVTASTRHEPPIFTINHHHSRPIIIGVPYVEQGIGQIDTREL
                     LNGTQLAVETINQQGGIHGREVQVATAGFYVKDKASILQAYHHLFDQEVDAISTAYAC
                     YLPEVHELIATKGIPYLHIATHSGSNKLAQNLPNQRIENMFQVCASDVTYGLGISHFL
                     QYYRYHYAPWLQNKTVMVVTVSWQKVDIGIESLTLSLREQGWNVDYLELTQEQDNYAT
                     LMAQVHNLAPSIIVFASYFAEDIVQFYQTFIRNPTNAIIYSIYAPSAFLPHEKLCEGV
                     LWSTNSGLSENYLGRQFSQHYERFFGKSPTYSQASIAYDQANILANAWKQSVSPRHFK
                     AVSNAIRLQPHYGVNGTYYFNTDSQIGLTYAETHDLSISLPQLVYQIQQGQSRVIAPE
                     LFANAQFILPPWFSEKA"
     regulatory      6546..6702
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            6691..7716
                     /gene="moaA"
                     /locus_tag="PXD81_001627"
     CDS             6691..7716
                     /gene="moaA"
                     /locus_tag="PXD81_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005607581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HDL5537639.1"
                     /translation="MQSIIPIKNVGDDVVSGLVDRFQRQYTYLRLSITDVCNFRCNYC
                     LPDGYKPPSHKQTFLSVDEIQRVAKAFANLGTEKIRITGGEPTLRKDFLEIAHTISQT
                     PGIRKVALTTNGYRLERDVELWQQAGITDINVSVDSLDPRQFQLITGENKLQSILKGI
                     DKAFEIGYPKIKVNAVLMKQYTAPELDKFLTWIKDKPIQMRFIELMETGEMDSFFQAQ
                     HLSGQSVMTRLLQEGWQLQPKAMSDGPAKVLSHPDFKGEIGLIMPYEKNFCASCNRLR
                     VSALGKLHLCLFGEEGIDIRDLLASDEQQLQLETRLKSSLQGKREHHYLHIGDSGVRN
                     NLASIGG"
BASE COUNT         2190 a         1710 c         1642 g         2411 t
ORIGIN      
        1 tttcccaaat taagttagaa atgaaattct tccgcccaaa aatttcatca agcataattt
       61 tcaaatagtg actttcgtta tcatcaagag tgatccaaat tgaaccatct tcggcaagta
      121 actctcgcag aatttcaagg cgtggataca tcattgaaag ccaaatggaa tgctctaaat
      181 tatcatcgta gtgggtaaag gccgattttg tgttataggg aggatcaata aaaatacatt
      241 tgacttgccc cgcataaaag ggcaaaagag attttaaggc tgataaatta tcaccctgaa
      301 taattaaatt ttgcttgact ttagataagg tttcagcaga agctgagctg tcggctgtcg
      361 gctgtcggct gtcggctgtc ggctgtcggc tgtcggctgt cggctgtcgg ctgtcggctg
      421 tcgggcaata ttatgcgaga agtccggcaa ctcacgcaat aggcaatatg gggtttgttt
      481 tgcttttttg acggcttctt ctttgccgta ccaagtaagg gttgtcataa aatgcacctg
      541 aaaagtaaaa gtggattatt gtaacaagcg gtcaaaaaaa tagaaagttt tacaaaaaat
      601 taagcaaatt taaccgcttg caacatagaa aaggcacagt tttatactgt gcctttggtt
      661 atatgaaatt agccttgtgt gtatttaggc tctttaaata ctgttacttc tggtgcttgt
      721 ccggtgaatt tttcaacctg taccgtagcg gtatttggtg agttaccctg tgctaatttt
      781 gatgtgcctt catcacgggt tagcacgtta gggttgccgt ttttacatag cggttgctcg
      841 ctggtgccga gatcgagcgg atcgtaccaa gctccttcgt gcaaggctaa tgtacctttg
      901 ataatgccat ctgtcaccac cgcaccggct aatacttgac cacgtttgtt aaaaatacgt
      961 acaatatcac cgcttgtaat gccacgagcg gcggcatctt cagtatggat caacaccggc
     1021 tcacggtcat taaccgcata ttgtttgcgt aatgatgagt aagctaactg gctgtgtaaa
     1081 cgccatttag agtgtggagt caccaatgcc aacggttctt ctgcggtggt tttacccgag
     1141 tattcttcgt gttccatcca agttgggtga cctttacagt catcataatt cattttggca
     1201 atcacctcag agaaaatctc aattttgccg gacggtgtgc ctaatggatt taggagtggg
     1261 tcattacgaa attcttcata acgaacccat tttttcgctt tgtctgatgc tttaaaagaa
     1321 agtggtttat tctcttgcca gaatttctcg aatttcggca taatcacacg atttttgcgt
     1381 gctgcctcaa atgcagtttg atagaaactt tgtagccaat ccatttcaga tttgccttcg
     1441 gtaaattccg cttctttgcc tgcacgtttg gctagttcgg caaaaatatc gtagtcgttt
     1501 tttgcctcaa attgcggttc gaccacctgt ttcatcggga aaatattcat cattgaataa
     1561 tcgcccgaca tagttaagtc attacgctca tagctggtgg ttgccggcag cacgatgtca
     1621 gacatacgag ccgttggtgt ccaatggcat tcattgacga taatagtatc cggtttttgc
     1681 cacgctttca ccaaagtgtt ggtgtcttgg tggtgggtaa acggattacc ccctgcccaa
     1741 tagacaattt tgatatcagg ataagtaatt tgtgtgccat tgtaatcaat ggttttgccc
     1801 gggttgagca gtgcatcgga aatacgagcc accgggaatg aagattttga tgtttcatct
     1861 aaccaggttt tcgctccggc ttctactgcc ggatttgctg tgatagagcc taaaatgccc
     1921 cctgtggcac tcggcacgcc gccgtttgag taatggtagc ttaaaccgaa accaccaccc
     1981 ggtaagccga tttgacctaa cattgcagct agggtgacta acatccaatg cccttgttca
     2041 ccgtgtcgtt ggcgttgaat cccccagcct gccattaaca ttgtgcgttt agattggaaa
     2101 tcagtcgcta attttttaat ggtttcttcc ggcacaccac aaatttgact tgcccatttt
     2161 gcatctttcg gttgattgtc tgttttaccg agcagatatt cttcaaactt ctcaaaaccg
     2221 gtggtgtatt ttttaaggaa tgcttgatcg tgtttgcctt cagcgactaa ggtatgagcc
     2281 ataccgagca ttaacgcaac atcagtgccg gtgtttaccg gtacccattc tgcacctaaa
     2341 aattcgcaag attcactttt taccgggtca atacagataa tgcgtttccc gctctctttg
     2401 agttttttga agtattcaat accttgttga tcggtagaag tccacgcaat gcgtagcgta
     2461 tttaacgggt tagctgacca aagcaccaca atatctgaac tttctaaaat cacttcccaa
     2521 ctagtttgct gttcgtacac ctcgattgta cccaagacgt gtggcataat cacttgagca
     2581 gcaccggtag aatagtcgcc ttttacacca acaaatccgc cggtaacatt taaatagcgt
     2641 tgtaacagtg tacgggaagc gtgtaatgcc ccagaactat accaaccgta agaaccacca
     2701 aaaatagcat tcgccccgtg agcctcacga acacgtttca tttcattcgc aactaattca
     2761 aatgccttat cccacgaaac tcgcacccat tcatcacgcc cacgtagagt agtatcttta
     2821 ttaccttcta agtagccttt acgcaccatt gggtatttta ctcgtgtttc accgtgtact
     2881 tggtctgcta ccacgtgttg caattcattt ggcacagaaa tctcaaaaac agaatcagat
     2941 ttcaccactt tgccattttc taccactaca cctaaaggcc cccagtgggc ggcagttact
     3001 actgttttca tctcggctgc tttggcactg gtggaagcta atgcccccac tacaccggta
     3061 gtaccactgg ctaaacctgc accggcagct aagcttaatg atgaattttt tagaaaaccg
     3121 cgacgactta cgtcaatatt gtcttgtttt ttcataataa acctcgtttg aagttaagtt
     3181 aaaaatagac acctttctaa agtcgagtgg ataaaccatt tctcgtgtat ccattggtaa
     3241 aagcaccgac ggtaattttt gcaaaatgtt aggaaaaaat gaccgcttgt agtaggtgct
     3301 ttacggcttg ctaccatatc tctgctctca gcagacactt aggctcacaa gctcggtatc
     3361 ctctaagttg caattaatgg gaactgcctg ctacatcttt tgcattacgt tggagataga
     3421 tagtcaccgc acgcacatca tcagccgaca tagatgttct gtctttcatt gaatttacca
     3481 ccccaatcca ttgatttgcg gtgtaatgtt ctgccccaat cggtgcatgg caaccgctac
     3541 agtgtgtttg gtttaagtta tcgccaaaat gatttagtgc agtaatattg gcagtgactg
     3601 catttttagg agcagtaatt tcaaaattga tttctttcca atttgagttg gtgacattat
     3661 caaacactgt ttttgtgaca ttcactttgc tttgagcgtc atcaggtaga agagcaacca
     3721 taatacgctt gcctaaatcc atatacacca cagattccgc accttcttgt tgccagccat
     3781 taaccgttgc tttaacctgc tcgccttctg ctgtccattg atgtaattct gcataaggta
     3841 acaagcgaat ttctccacct tgagcgagct gcactggagt cattgcgagc gtataaaggg
     3901 ttttatcatt tgtgctaaat tccgcaccgt gtttagctaa ttcgcccatt gctgcggcat
     3961 tatctaccgg cacatcaggc ataaaatggg caattccttt atggcaatca atacaggttt
     4021 gattagtttc ttgggctagt ttgtgcattt tctgtgccgc ttctgtttgt tcagaaagta
     4081 ccatcgcatc aaaactatga cagcttttac aggtcataga atcgttttct ttcatttctg
     4141 cccaaacacg ttgtgccatt tctagacgat gggcttcata ggcttcttga tcaggtaatt
     4201 tattggttac aacggtgtgc caaacatctt ttaatgcgat gattttagct ttgacataat
     4261 gcaagccgtc ttgtggtaga tggcaatccg aacactcggc tcgaattccc ttacggttgg
     4321 aaaaatggac actgccttcc cactctgcct gtgggtgagt catagaatgg cagctcacac
     4381 aaaattctgt tgagctggtg gctttcattg catattgtgt gccacctaat accgcagcac
     4441 ctaatgccat aataccgaca aatacagcaa tttttttcat cttagccata aattacccca
     4501 cttaatacac taaaaattaa agacagaggc attgtattaa ctcccaagag gtataaaaat
     4561 aagtaaaaat acctattacc tcgaaagagt gagaataatt tgatctaagg caaaaatatg
     4621 tagtgaaatt ttaggctttt tcagagaacc acggcggtaa gataaattgt gcattggcaa
     4681 acaactccgg tgcaatcaca cggctctgcc cttgttggat ttggtaaacc aactgtggca
     4741 agctgataga taagtcgtgg gtttctgcgt aagttaatcc aatttggcta tcggtattaa
     4801 aatagtaagt gccgtttacc ccataatgtg gctgtaagcg gatcgcattg gagacggctt
     4861 taaaatgccg tggtgaaaca ctctgtttcc acgcatttgc cagaatattc gcttggtcat
     4921 aagcaatact ggcttgggaa taagtgggcg atttgccgaa aaaccgttcg taatgttggc
     4981 taaactgtct gcctaaataa ttttcgctta acccactatt ggtactccaa agaacgcctt
     5041 cgcaaagttt ctcgtgtggc aaaaacgccg aaggtgcata aatactgtaa ataatggcat
     5101 ttgttgggtt gcgaatgaac gtttgataaa actgtacaat atcttctgca aaataagagg
     5161 caaacacaat aatggaaggt gcgagattat gcacctgtgc cattaatgtg gcataattat
     5221 cttgctcttg ggtgagttcc agataatcca cattccagcc ttgctcccgc aaacttaggg
     5281 ttaaactttc aataccgata tcaacttttt gccaactcac cgttaccacc atcaccgttt
     5341 tattctgaag ccaaggggca taatggtaac ggtaatattg cagaaagtga gatataccta
     5401 agccataggt tacatcactg gcgcaaacct gaaacatatt ttcaattcgt tgattcggta
     5461 aattttgggc aagtttattt gagccactat gggttgcaat atgcaaataa ggaatgcctt
     5521 tagttgcaat taattcgtgt acttccggca agtagcaggc ataagcggtg gaaatcgcat
     5581 caacttcttg atcaaataaa tgatggtatg cctgaagaat acttgcttta tctttaacat
     5641 aaaacccagc cgttgcaacc tgtacttctc tgccgtgaat accaccttgt tgattgatgg
     5701 tctcaaccgc aagttgagtg ccgttaagta gctctcgggt atcgatttgc ccaataccct
     5761 gttcaacata aggcacgcca ataataatcg gacgactatg atggtggttt atcgtaaaaa
     5821 tcggtggttc gtgtcgagtt gaagccgtta caagcggttg ttttttcgcg ataatttgca
     5881 atgccagttg ttcaatgtta aagctggcaa gaatggattg ggttagtttt cccggtgtgg
     5941 gtaaacagat taaatgttcg gtaatggcaa aaacagctaa taaggttcgg cttttggtat
     6001 gtgttttcgc aaaaatattt tcgatatgtt tatcaacagt ccgtttgctg atacaaagct
     6061 cattggcgat atcctcattg ctcaaaccac tgctaattag agtgagaagc tcaatctccc
     6121 ttttactcag tccataaggc aaatcggtat gctctgcttg agtcaataca aacgattggt
     6181 ggcgttctag gctgatctta agccattgat ggtttgaact atagtaaaac tgcaagctat
     6241 ttttatctga atagagcaat gcctgtgcat agtggcaaat ttgcggtaat tcagcaaaat
     6301 agtctgcccc tgctttgtct tcccattctg atatttcgcc ttgttcattt actaaacaag
     6361 accatcggct acgtttatcc attctcacct cgaactaaaa acgttagcta aaatagcaaa
     6421 attatctcgg cattaatatg atataaatca aactaagcaa cggttgccag atttttatag
     6481 gtagagcata caatttaaat tatacctctt gcctaattag gtttctcacc ataaaatgaa
     6541 aaccaattta ttgaagactc cgagcattat ttcctaactc taaaaattaa agacaaattt
     6601 ttgatatgga atttatgcca gtaacagcat aaagctgttc aaaggataac ttagaaatgg
     6661 gttgtcctct cgtgtttaga aaggtgtcaa atgcagtcta ttatcccaat taaaaacgtg
     6721 ggtgatgatg tcgtttcagg tttagttgat cgctttcagc gtcaatatac ttatttgcga
     6781 ctatccatta ctgatgtttg taacttccgt tgtaattatt gcttgcctga tggctataag
     6841 ccgccttcac ataagcaaac ttttcttagc gttgatgaaa tccagcgggt ggctaaagcc
     6901 tttgccaatt tagggacgga aaaaatccgt atcaccggtg gtgagccaac tttacgcaaa
     6961 gatttcctcg aaatcgctca cactatttca caaacacccg gtatccgtaa agttgcccta
     7021 accaccaacg gctatcgcct agagcgtgat gttgaactgt ggcaacaagc cggtattacc
     7081 gatattaatg tgagtgttga tagccttgac ccacgccaat ttcaactgat taccggcgag
     7141 aacaagttgc aatctatttt aaagggtatt gataaagcct ttgaaattgg ctacccaaaa
     7201 attaaagtca atgcggtgct aatgaagcaa tataccgccc ctgaattgga taaattcctc
     7261 acttggatca aagacaagcc gattcaaatg cggtttattg agctgatgga aaccggtgaa
     7321 atggatagtt tcttccaagc tcaacatctt tctggtcaat cggtgatgac ccgcttatta
     7381 caagaaggct ggcaattaca acccaaagcg atgtctgacg gcccggcaaa agtgctttct
     7441 caccctgatt ttaaaggcga aatcggctta attatgccct acgagaaaaa tttctgtgcc
     7501 tcttgcaacc gcttgcgtgt ttccgcactt ggtaaactgc atctttgctt atttggtgaa
     7561 gaagggattg atattcgtga tttactggca agcgatgagc aacagttaca gcttgaaacc
     7621 cgcctaaaat cttccttaca aggcaagcga gaacaccact atttgcatat tggggatagt
     7681 ggggttagga ataatttggc gagtatcggt ggttaaacaa ccaccgagcg tgttttttct
     7741 gttacttttc tttgcttcgc caaagaaaag taaccaaaag aaaggcgacc ccagattttc
     7801 cttaatcctt cgcttgttgc taacttttgg gacgaacttt tcaaactcgc caactgcgtt
     7861 ggctcaaaca cgaaaagttg tcctcaaaag tttacgcaat cgctacggcg gaaaataggg
     7921 gcaagcggta cattatttaa gatttttgca agt
//
LOCUS       DANLYI010000106         7936 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.106, whole genome shotgun sequence.
ACCESSION   DANLYI010000106 DANLYI010000000
VERSION     DANLYI010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 7936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7936
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.106"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            112..1020
                     /locus_tag="PXD81_001628"
     CDS             112..1020
                     /locus_tag="PXD81_001628"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HDL5537640.1"
                     /translation="MNKNSLFSIWFTTARPKTLPLALASIIVGSALAHWAGKFDVVTT
                     LLAFITTILLQILSNFANDYGDHVKGSDTKERIGPLRAIQHGVIGGEQLKKAVIILSL
                     LSFVSGVALSAYAFETVQDLFFFIGLGIVSIIAAITYTVGKKAYGYLGLGDLFVLIFF
                     GFVAVIGVFYLQAHSLPIMILLPAFGCGLLSVAVLNINNLRDINQDRQAGKNTLIVRI
                     GSQNGRKYHVALLVLAVISYLIFAISEFEHWYSFIFLLSVPLLAKHARFVYQHKDPKE
                     LYPILGQMAGLALLTNLLFSLGIVLG"
     gene            complement(1096..2082)
                     /locus_tag="PXD81_001629"
     CDS             complement(1096..2082)
                     /locus_tag="PXD81_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HDL5537641.1"
                     /translation="MKKSLLALLIGSVFLISACEDGKMTQTVLEAEKQIVQLGTELKN
                     AQADLAKKEAEVAELSGVKAENEKLKSELENAQQNTALNVEIVKIFDKKEVIKHQVDP
                     KQEFAIEESTVSAFVSLPKTNFDWLNQLLINQAYDYNEEQGRKLKNPTEEEFKKHREA
                     VYQGLVESVKTEPAIGYDDHISSAFIGQRNNIATFTMMHYTYMGGAHGMHYTQYINVD
                     LAKKAIIGLNDLISPKNQVQLKDILWENYSSVRVDENGNYNGFADKKEFRISDQFYFT
                     PHGIAFVYPPYELGAYAEGDIEVEADWYIINKLLNSEYQRGEKDGFFEELDN"
     gene            complement(2176..4047)
                     /gene="rpoD"
                     /locus_tag="PXD81_001630"
     CDS             complement(2176..4047)
                     /gene="rpoD"
                     /locus_tag="PXD81_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HDL5537642.1"
                     /translation="MENQADQQLEQQSQLELLISQGREQGYLTLSEVHDHLPEELVDA
                     EQIEDIIQMINDMGIQVLEAAPDADDLLLAENIADEDVVEEATKVLSTVESELGRTTD
                     PVRMYMREMGTVELLTREGEIDIAKRIEEGINEVQCAIAEYPEALSGLLACYAQVETG
                     EMRLSDLVTAFVDPNILEEAPLEDIEAALGEDEESSDVPDVDDENEDENGDSSSDSSD
                     DSSIDPELAREKFEALRSQHEKVLLVIQKHGRTSKKSKEQIEILSHIFREFRLVPKQF
                     DLLVASMQKLMKQVRTEERQIQRYAVEYAGMKKADFLKAFQGHELSEAWIEKAINAKK
                     NAAPKLANYVDNIRVNIANLQNLEQTSGLTIAQIREIGGRISNGELKARRAKKEMVEA
                     NLRLVISIAKKYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAIT
                     RSIADQARTIRIPVHMIETINKLNRISRQCLQEMGREATPEELAERMGMPEDKIRKVL
                     KIAKEPISMETPIGDDDDSHLGDFIQDESLELPLDSATAESLRMATNEVLEGLTPREA
                     KVLRMRFGIDMNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSETLRSFLDD"
     gene            complement(4306..6054)
                     /gene="dnaG"
                     /locus_tag="PXD81_001631"
     CDS             complement(4306..6054)
                     /gene="dnaG"
                     /locus_tag="PXD81_001631"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HDL5537643.1"
                     /translation="MKGTIPRSFIDDLIARTDIVEVINSRVKLKKAGRDYQACCPFHH
                     EKTPSFSVSQSKQFYHCFGCGVHGNVITFLMDYDKLEFPEAIEELAAMHGLEVPRENV
                     IQRDGKPQANFKTKRNLYELMEAVAKFYQQNLSHQIEPQSYLQQRGLSEEIIERFEIG
                     FAPNAFDAALRTFGQSQEDIQKLLDTGVLTKNDSGRIYDKFRNRVMLPIRDKRGRVIA
                     FGGRVLPNDEQGAKYMNSPETVIYHKGSELYGLYQALKVNETPDYLFVVEGYMDVVAL
                     AQFGVDNAVASLGTATTGEQIQQMFRCTEQIVCCYDGDRAGRDAAWRALENALPYLQD
                     GRQLKFIFLPDGEDPDTFVRSQGKEGFEQYVAQNAKPLSDFLFDSLLADVDLSSKEGK
                     SKLAALAVPLINRIPGEMLRVYLRNILGQKLGILDPTQIEALLPTKKAAEKPLHQAVK
                     MKRTPMRLLIALLIQNPYFVKFVPDVSALASLKDPGFTLLTDIVATCQQNAGISMGGL
                     LEHYREDPQYKIVEALANWDHLELPENIERTFIDTLDFLYAKLVELRKEELIAKDRTV
                     GLTDDEKKEYALLMFQ"
     gene            complement(6208..6423)
                     /gene="rpsU"
                     /locus_tag="PXD81_001632"
     CDS             complement(6208..6423)
                     /gene="rpsU"
                     /locus_tag="PXD81_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005701713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HDL5537644.1"
                     /translation="MPVIKVRENESFDVALRRFKRSCEKAGILAEVRAREFYEKPTTI
                     RKREKASLAKRHAKRNFRENARNTRLY"
     gene            complement(6571..7167)
                     /locus_tag="PXD81_001633"
     CDS             complement(6571..7167)
                     /locus_tag="PXD81_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HDL5537645.1"
                     /translation="MSLLPMNEITSLNQLDLQKSYSYADYLLWKFKERVEIIKGKIVA
                     MSPAPTRFHQRISMKMTAKFLEVFDNHQCQIYSAPFDVRFPDENGKVKTVVQPDLCVI
                     CDVSKLDERGCVGAPDLVVEILSPGNTKREMKDKYELYQEQGVKEYWIVSPETRTIQI
                     FVLENGKYIGIQPVAEDEPATSVVFPALSFSTEKLFEL"
     gene            7309..>7936
                     /locus_tag="PXD81_001634"
     CDS             7309..>7936
                     /locus_tag="PXD81_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HDL5537646.1"
                     /translation="MHLNFKKIDNSHIALFYNVLVATFFVTVLTFKKGYSYVPMALGI
                     IATLSFLFYRFKLKQKWLLDKEDKFFIFSLIGYFLTFAMSAIFNGDGIREIDNPIQIL
                     LLIPLIILFRLYPIKKETVLHFIPIGALVAGCIAIYQRFILNLKFPFPEIMRIQSGNI
                     AILLSLFSIAIAFYWLSKKRIKTGIAYLVFAMFGLFASILTGARGGWLA"
BASE COUNT         2178 a         1762 c         1453 g         2543 t
ORIGIN      
        1 cgtccacgct tgtaccaatt cgttttacca tttaacacaa gagtggacgc ttgcactatc
       61 gcttaaatcc ttaacatcat tgtaaatgaa attacgctaa gattattctc tatgaacaaa
      121 aattctctct tttcgatttg gtttaccacc gctcgcccga aaactttacc gctggcgtta
      181 gcttcaatta ttgtgggttc agcacttgct cattgggcag ggaaatttga tgttgttacc
      241 accttactcg catttatcac caccattttg ttgcagatcc tgtctaactt cgccaatgat
      301 tacggcgacc acgtcaaagg ctctgacacc aaagaacgaa tcggaccgct acgggcgatt
      361 caacacggtg ttattggcgg tgaacaactt aagaaagccg tgattatctt aagcctactc
      421 tcgtttgttt ccggtgttgc cttaagtgcc tatgcttttg aaacagtgca ggatctgttt
      481 ttctttatcg ggcttggcat cgtctcaatt attgccgcca ttacttacac cgtcggcaaa
      541 aaagcctacg gctatttggg gctgggcgat ctatttgtgc tgattttctt tggctttgta
      601 gcggtaatcg gcgtgttcta cttgcaggct catagcctac ctataatgat tctgctccct
      661 gctttcggtt gcggattgct gtctgtagca gtgttaaata tcaataactt gcgtgatatt
      721 aaccaagacc gacaagctgg gaaaaacact ctcattgtac gaatcggtag ccaaaacggg
      781 cgaaaatacc acgttgcatt gttagtgcta gcggtcattt cttatctgat ttttgcaatt
      841 tctgaatttg aacattggta tagcttcatt tttttgctgt ctgtaccgtt actggcaaaa
      901 cacgctcgtt ttgtctatca gcataaagac ccaaaagaac tctacccaat tctagggcaa
      961 atggcagggc tggcgttgct cactaatcta ttatttagtt tgggcattgt gttagggtaa
     1021 gttgcaaaat tttaacaaaa ctaaaccgct tgtaataaag gattacaagc ggttgttttt
     1081 tatctaaatt tacaattagt tatctagttc ttcaaagaat ccatcttttt cgccacgttg
     1141 atactccgaa ttgagcaact tattaataat gtaccaatcc gcttcaacct ctatatcgcc
     1201 ttctgcataa gcacctaatt cgtatggcgg ataaacaaaa gcaatgccgt gaggggtaaa
     1261 gtagaattgg tcagaaatac ggaactcttt tttatcagca aatccattat agttgccatt
     1321 ttcatccaca cgcacgctac tgtaattttc ccaaagaatg tctttcagtt gaacttgatt
     1381 ttttgggctg attaaatcat ttaagccaat gattgctttt ttagctaaat ctacattgat
     1441 atattgcgta tagtgcatac cgtgagcccc acccatatag gtgtaatgca tcatagtgaa
     1501 tgtagcaata ttattgcgtt gcccgataaa agcactggaa atatgatcat catagccaat
     1561 agctggctcg gttttcacac tttctaccaa cccttgataa accgcttcac gatgcttttt
     1621 aaactcttct tcggttggat ttttcagctt tctgccttgt tcttcattgt aatcataggc
     1681 ctgattaatc aaaagctggt taagccaatc aaaattggtt tttggtagag acacaaacgc
     1741 acttactgta ctttcttcta ttgcaaattc ttgtttcgga tcaacttggt gcttaatcac
     1801 ctctttctta tcaaagattt tcacaatttc aacatttaac gctgtgtttt gctgagcgtt
     1861 ctctaactct gattttaatt tttcattttc tgctttcacc cctgaaagtt ctgcaacttc
     1921 agcctctttt tttgctaaat cagcctgagc attttttaat tctgtgccta actgaacaat
     1981 ttgtttttcg gcttctaata ccgtttgggt cattttgccg tcttcacaag ctgaaatcag
     2041 aaatactgag ccaattaaaa gtgctaatag tgattttttc atctctccct cttttgtaaa
     2101 aaatacggta aatatcaccg cttgttatac agattaaaac aaaatcccct ataccatcag
     2161 gcatagggga aatgattagt cgtctaagaa actgcgtaag gtttccgaac ggctcgggtg
     2221 gcgaagtttg cgcagtgctt tcgcctcaat ttggcggata cgttcacgag ttacgtcaaa
     2281 ttgtttacca acttcttcga gtgtgtggtc tgtgttcata tcaataccaa aacgcatacg
     2341 caacactttc gcttcacgcg gggttaagcc ctctaacact tcgttcgttg ccatacgcag
     2401 gctctccgca gtggcggaat ccaatggtaa ttcaaggctt tcgtcttgaa tgaaatcacc
     2461 taaatgtgaa tcgtcatcgt caccgattgg tgtttccatt gaaatcggct ctttggcaat
     2521 tttcaatact ttgcggattt tatcttccgg cattcccata cgttctgcca gttcttccgg
     2581 cgttgcctca cgccccattt cttgtaaaca ttggcgtgaa atacggttga gtttgttgat
     2641 agtttcaatc atatgcaccg gaatacgaat tgtacgggct tggtcagcaa tcgaacgggt
     2701 aatcgcttga cgaatccacc aagttgcata agtcgagaat ttataaccac ggcggtattc
     2761 aaatttatcc accgctttca ttaaaccgat gtttccttct tggattaaat ctaagaactg
     2821 caagccacgg ttagtgtatt ttttcgcaat cgaaatcacc aaacgtaagt tagcttccac
     2881 catctctttt ttcgcacggc gggcttttaa ctcaccattg gaaatacgcc cgccaatttc
     2941 acgaatttga gcaatggtta agcctgatgt ttgctctaaa ttctgtaagt tagcaatatt
     3001 gacacggata ttatccacat aattagctaa tttcggtgct gcatttttct tggcattaat
     3061 cgctttttca atccacgctt cgcttaattc gtgcccttgg aatgctttca agaaatccgc
     3121 ttttttcatg ccggcatatt ccactgcata gcgttgaatt tgacgttctt cagtgcgaac
     3181 ctgcttcatc agtttttgca tagaagcaac caataagtca aattgcttcg gcaccaaacg
     3241 gaattcacgg aaaatatgag ataaaatttc aatttgctct ttcgattttt tactggttcg
     3301 cccgtgtttt tgaatgacca ataacacttt ttcatgttgg ctgcgtaggg cttcaaattt
     3361 ttcacgggca agttccgggt caattgagct gtcatcactg ctgtctgaac tgctatcacc
     3421 gttttcatct tcgttttcgt catctacatc aggcacatca gaagattctt catcttcacc
     3481 taaagcagcc tcaatatcct ctaacggtgc ttcttctaaa atattaggat cgacaaatgc
     3541 tgtcactaaa tcagaaaggc gcatttcacc ggtttcgact tgagcatagc aggctaacag
     3601 gccacttaag gcttccggat attccgcaat cgcacattga acttcgttaa tcccttcttc
     3661 aatgcgttta gcgatatcaa tctcaccttc acgggttaaa agctcaactg tccccatctc
     3721 acgcatatac atacgcacag ggtcagtagt tctacctaac tctgattcta cggttgaaag
     3781 cactttggtg gcttcttcta ccacatcttc atcggcgata ttttcagcga gtagtaaatc
     3841 atccgcatca ggggcagcct ctaaaacttg gatacccata tcgttgatca tctgaataat
     3901 atcttcgatc tgctccgcat ccaccaactc ttccggtaag tggtcgtgca cttcagaaag
     3961 cgttaaatat ccctgttcac gcccttgact gatcagaagt tctaattgag attgctgttc
     4021 taattgttga tctgcttggt tttccatagt ctatccgttt attatgcgag caaaaatagt
     4081 agtggattat accactaaat ttggttcgac ttcataaatt cacaaaaaaa ttctattttt
     4141 ctaatttaca cattactttg atgcttatga gaacagagag gtgtagcaag agagataaat
     4201 aactttcttt ttagatagaa ttaattattt tcttcgttca tcacttctgc caaatctccc
     4261 ctaacccctc tttgctaaag aggggaatta ttcgagctta aaattctact gaaacatcaa
     4321 aagagcatac tcttttttct catcatcggt taagcccacg gttcggtctt tggcaattag
     4381 ctcttctttg cgtaattcca ctaatttggc gtagagaaaa tcgagagtgt caataaatgt
     4441 tcgttcgata ttttcgggta actctaaatg atcccaattt gcgagggctt caacaatctt
     4501 atattgggga tcttcccgat aatgttcaag tagcccgccc atactgatac ctgcattttg
     4561 ctgacaagtt gcgacaatat cggttaataa tgtaaagcca ggatctttca aggaggctaa
     4621 agcacttaca tcgggtacga atttcacaaa atacggattt tgaatcaata acgcaatcaa
     4681 cagccgcatt ggcgtacgtt tcattttgac cgcttgatgt aaaggcttct ctgccgcttt
     4741 tttcgtcggt aacagagctt ctatctgcgt tgggtctaaa atgcctaatt tctgtcctaa
     4801 aatattgcgt aaatacactc gcagcatctc gcccggaata cgattaatta acggcacagc
     4861 taaagcagcg agcttggatt ttccctcttt tgaagagaga tccacatccg ccagcaaact
     4921 gtcaaataag aaatcgctta atggcttggc attctgtgcc acgtactgct caaatccttc
     4981 tttaccttga gaacgaacaa aggtgtccgg gtcttcgcca tcgggcaaga aaataaattt
     5041 cagttggcga ccgtcttgca aataaggcaa cgcattttct aacgcccgcc aagctgcatc
     5101 tcgaccggct ctatcgccat cataacaaca gacaatttgc tcggtgcaac gaaacatttg
     5161 ctgaatttgt tcaccggtag ttgccgtgcc taaagaggcg actgcattgt ccacgccgaa
     5221 ttgggcaagg gcgaccacgt ccatatagcc ttccaccacg aacagataat ccggcgtttc
     5281 attgactttc agtgcttgat acaagccata taactcactg cctttatgat aaatcaccgt
     5341 ttcgggcgag ttcatatatt tcgccccttg ctcatcattc ggcaacaccc gcccaccaaa
     5401 ggcaattacc cgtccacgct tatctcgaat cggtaacatt acccgattgc gaaatttatc
     5461 gtaaattcga ccgctatcat ttttggttaa aacgcccgta tcgagcaatt tctgaatatc
     5521 ttcttgtgat tgcccaaaag tgcgtagggc tgcatcaaag gcattcggag caaaaccaat
     5581 ttcaaagcgt tcgataatct cttcagacaa acctcgttgc tgtaaatagc tttgtggctc
     5641 aatctggtgc gataaattct gctggtagaa cttcgccacc gcttccatca attcataaag
     5701 attacgctta gttttaaaat tagcttgagg cttgccgtca cgttgaatca cattctctct
     5761 cggtacttcc agcccgtgca tagctgccag ttcttcaatc gcttccggaa attcgagttt
     5821 gtcataatcc atcaaaaagg tgatgacatt gccgtgtacg ccacagccga aacaatggta
     5881 aaactgtttt gattggctta ccgaaaagga aggggttttc tcgtgatgaa aaggacagca
     5941 ggcttgataa tctcgcccag cctttttgag tttgacccga ctgttgatga cctcaacaat
     6001 atcggtgcga gcgataagat catcaataaa tgaacgtgga attgtgcctt tcattgattt
     6061 cctcctgctc tttgaatgat acaagcggag caattttgca aattttttgc aaaaaacaac
     6121 cgcttgaaat agaaacgtta aaaacaccaa aaccgtgagc ctaacggaat cacggtttcg
     6181 atatagtttg ctaaccagcc taactaatta gtataaacgt gtgttacgtg cgttctcacg
     6241 gaaattacgt ttagcgtgac gtttagcaag tgatgctttt tcacgtttac gaattgtagt
     6301 tggtttttcg tagaattcac gagcacgaac ttcagctaag atacccgctt tttcgcaaga
     6361 gcgtttgaaa cgacgtaatg ctacgtcaaa tgattcattt tcacgaactt taattactgg
     6421 catatgccat cacctcagaa aaatatgatt aaattaaacc gcacacaatt ttggcggcat
     6481 agaacaaaaa aggtagtgca ttctacctta agcactcaca taagtaaagt agtattgtgg
     6541 cataaaaccg ccgtattatg ttgcattttt ctacagttcg aataatttct cggtcgaaaa
     6601 acttaatgcc ggaaagacca ccgaagttgc cggttcatct tctgccaccg gctgaatacc
     6661 gatatattta ccgttttcca acacaaaaat ctgaatagtg cgggtttcgg gagaaacaat
     6721 ccaatactct ttcacccctt gttcctggta aagctcgtat ttatctttca tctccctttt
     6781 ggtattaccc ggagaaagga tttccaccac taaatccggt gcccctacac agcctctttc
     6841 atctaactta ctgacatcac aaattacaca aagatcaggc tgcactacag ttttgacttt
     6901 tccgttttca tcgggaaacc gaacgtcaaa aggcgcagaa taaatctggc attggtgatt
     6961 gtcaaaaacc tctaaaaatt ttgctgtcat cttcatagaa attcgctgat gaaaacgagt
     7021 aggagccgga gacatcgcca caattttccc tttgataatc tcaacccgct ctttaaattt
     7081 ccatagcaaa taatcagcgt aactgtaact tttttgtaag tccagttgat ttaagctggt
     7141 aatttcgttc attggtaata agctcatacc gcctccttta tcagaaatat aaagatatta
     7201 tgccgaataa tccctcaaat tcaacaaaac tttcgctaaa aagagataga taacctttat
     7261 acccttagta taaaatgaca gcaattttca ttgttttagg atatgtcgat gcaccttaac
     7321 ttcaaaaaaa tagacaactc acatattgct cttttctata atgtactagt cgccacattt
     7381 tttgtcactg ttttaacctt taagaaaggt tatagttatg tgccaatggc attaggtatt
     7441 atcgctacac ttagtttctt attttatcgt ttcaaattaa aacaaaagtg gttacttgat
     7501 aaagaggata aattttttat tttctcgctt attggctatt ttctaacgtt tgcaatgtct
     7561 gctattttta atggcgatgg cattcgtgaa attgataatc ctatccagat tttattgctt
     7621 atccctttaa tcatcctctt tagactttac ccgattaaaa aagaaactgt attgcacttt
     7681 ataccgattg gagcgttggt agctggttgt atagcaatat atcagaggtt tatattaaat
     7741 cttaaatttc ctttccctga aataatgcgt attcaatcgg ggaacatagc aatattatta
     7801 tcactattta gtatagcaat tgccttttat tggctttcaa aaaaaagaat taaaactggc
     7861 attgcctacc tggtttttgc catgtttggg ttatttgcca gtattctaac aggagcaaga
     7921 ggcggctggc ttgcat
//
LOCUS       DANLYI010000107         7904 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.107, whole genome shotgun sequence.
ACCESSION   DANLYI010000107 DANLYI010000000
VERSION     DANLYI010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 7904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7904
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.107"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            complement(52..1092)
                     /locus_tag="PXD81_001635"
     CDS             complement(52..1092)
                     /locus_tag="PXD81_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HDL5537647.1"
                     /translation="MQTTLSPQQIAKKHRTIEKKRSFLLLFFLLICLLSLIFDIMTGP
                     SLLPFNEVWKALFQLGEIEETTKVIVYELRLPMAIMALVVGAALGVGGAEIQTLLNNP
                     MASPYTLGLAAAAGFGASIVIAFGGFGLPTEISVPVGAFVMTLFSSAILFAFSSLGRF
                     SSAILVLVGIALLFLFQSLLSLVQFISAPEVSQQILFWLFGNLTKATWQTISIAFGVT
                     TVCILLLFKDAWKLTALRLGEARALSLGINLTWLRMKTLLIVSVMTATCISFVGIIGF
                     IGLVAPHIARMLLGEDQRFFLPGSMLVGAAFLSMSSVLSKSLVPGALFPVGIITALVG
                     VQFFFWIVLAKR"
     gene            complement(1181..1771)
                     /locus_tag="PXD81_001636"
     CDS             complement(1181..1771)
                     /locus_tag="PXD81_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein phosphodiesterase"
                     /protein_id="HDL5537648.1"
                     /translation="MNFLTHSLISLEIDTQTKQQTLYGNFAGDFYKGLIPHLNLADNL
                     KSGVVLHRSIDKISDRPDSLTTQLLQGTFGRYKGIVSDIMIDHFLAKHFQRLFAKDLN
                     EVQAQIFQQIEQQRTIFPEQFVPMFNWLQSRKALTHYADLDFLEQVFLGMSSRVKKGE
                     ILRNAVTEIWQNYTLLEEAAIQEFFTVKDRATRELS"
     gene            complement(1772..3145)
                     /gene="modF"
                     /locus_tag="PXD81_001637"
     CDS             complement(1772..3145)
                     /gene="modF"
                     /locus_tag="PXD81_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HDL5537649.1"
                     /translation="MVVDGNGSGKTTFSLALQGKISLYSGDYGNSFQHIQLLSFEQQQ
                     KMLEQIFKDRNNDSVSPDDFGLTARQVILNGSPKEALCEEYAHKLHIQHLLERPFMHL
                     STGESRKVLFCQMLVSEPDLFILDEPFEGLDQQSVQYWQQLIAELQSKMALVLIVNRF
                     NDIPDAANHIELLDNLSLILQGERAEIEQQAVYSQLKFAEENVNVPLPDSAAPLLSLP
                     ENINPFELENVNIQYGEKKILDNLSWTVQPKQHWWIKGPNGAGKSTLLSIITGDHPQS
                     YANKVRLFGKQRGSGETIWDIKKNIGYVSSQLHMDYRVNCSAMEVILSGFFDSIGVYQ
                     QVPNALQLKAMEWLERLNLSNVAKKPFRSLSWGQQRLLLITRAMVKHPSILILDEPLQ
                     GLDGINRKLVKQFIEQLVTNSQTQLLFVSHQDSDAPNCITHLFEFVPKGETNEFGFEY
                     HQTALGA"
     gene            complement(3263..4216)
                     /gene="fmt"
                     /locus_tag="PXD81_001638"
     CDS             complement(3263..4216)
                     /gene="fmt"
                     /locus_tag="PXD81_001638"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HDL5537650.1"
                     /translation="MSKLNIIFAGTPDFAAQHLQALLDSEHNVIAVYTQPDKPAGRGK
                     KLQASPVKQLAEAHNIPVYQPKSLRKEEAQAELKALNADVMVVVAYGLILPEAVLNAP
                     KYGCLNVHGSLLPRWRGAAPIQRSIWAGDTETGVTIMLMDVGLDTGDMLHKVTTPIEP
                     NETSASLYAKLAELAPLALLEVLNGLANHTFKAEKQDEALSNYAEKLSKEEAKLDWQL
                     PAVQLERNIRAFNPWPFSFLTLTVDGVEQSIKIHQATVLPHQEKAAGTVINVDKTGIQ
                     IATQQGVLNITQLQPAGKKPMSVQDFLNGRADWFVSGTQLS"
     gene            4331..4942
                     /locus_tag="PXD81_001639"
     CDS             4331..4942
                     /locus_tag="PXD81_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HDL5537651.1"
                     /translation="MEFFIPKADTIFEEEIKKSRFITYLRHTEGMEQAKAFWQELKIQ
                     HPHARHWCWATVAGLPNDSQQYGFSDDGEPSGTAGKPMLNYLLGSGLGEITAVVVRYY
                     GGIQLGTGGLVKAYGNGVQQALLQLERIRKVLRKNYRLQCEYEQFNTISHLISEKDIV
                     IVEQLFSDKVDLLLAFNPSEVEAFADELTQRFSGKLALIKEEI"
     gene            4946..6430
                     /locus_tag="PXD81_001640"
     CDS             4946..6430
                     /locus_tag="PXD81_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015674320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter"
                     /protein_id="HDL5537652.1"
                     /translation="MQFFSIIRIVGILVMCFSFTMLVPATVALIYGDGGGRAFLEAFA
                     LNFMVGTLLWWLCRDNKNELRSREGFLIVVLFWVVLGLLGAVPFIILENPDLNVAESI
                     FESFSGLTTTGATVITGLDSLPKAILFYRQFLQWLGGMGIIVLAVAIIPLLGIGGMQL
                     YRAEMPGPLKEQKMRPRIAETAKALWLIYLSLTILCAVAFWLAGMTVFDAISHSFSTV
                     SIGGFSTHDASIGYFNNAAISYITVFFLLLSACNFALHFAVIDRIRDAQSRKKGGILS
                     RLYWGDYEFRFFLMVQLGLFLVCTLVLVGYGYFDNTSVTIEQALFQSVSISTTAGFTT
                     NDFSQWSSFLPTLLVLSSFIGGCAGSTGGGLKVIRVLLLYLQSKREIHRYIHPNVIQP
                     IKLGNHVLSERVVDGIWAFFSAYFFVFVICWLATIACGMETFDALNAVIASLNNLGPA
                     LGSVSSNFTQVPDSAKLVLTFAMVCGRLEVFTLLVILSPVFWKD"
     gene            6431..6958
                     /gene="hemG"
                     /locus_tag="PXD81_001641"
     CDS             6431..6958
                     /gene="hemG"
                     /locus_tag="PXD81_001641"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HDL5537653.1"
                     /translation="MKTLILYFTTDGQTKKIAERLADVITHEVELISLKDQAVDFAEK
                     IANADQIVIGASIRYGHFNPLVYQFVEQHYPALNAKKTAFYGVNLTARKDNRNTPETN
                     TYVRKFLAKVKWKPTHIEVIAGALLYLRYKPFDRLMIRFIMKITKGETDTSKEYEYTD
                     WQQVENFGKLLANQL"
     gene            7090..7485
                     /gene="mscL"
                     /locus_tag="PXD81_001642"
     CDS             7090..7485
                     /gene="mscL"
                     /locus_tag="PXD81_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015674322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HDL5537654.1"
                     /translation="MSILKEFREFAVKGNVVDMAVGVIIGGAFGKIVSSLVSDVVMPP
                     IGWLIGGVDFKDLAIEIAPAKEGAEAVMLKYGAFIQNVFDFLIIALAVFGMVKVINSL
                     KKAPVVEEAPAEPTAEEKLLTEIRDLLKK"
     gene            complement(7598..7861)
                     /gene="rpsT"
                     /locus_tag="PXD81_001643"
     CDS             complement(7598..7861)
                     /gene="rpsT"
                     /locus_tag="PXD81_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HDL5537655.1"
                     /translation="MANIKSAKKRAVQSEKRRQHNASQRSMMRTFIKKVYAAVAAGDK
                     AAAQTAFVEMQKVVDRMASKNLIHANKAANHKSKLAAQIKKLA"
BASE COUNT         2181 a         1583 c         1778 g         2362 t
ORIGIN      
        1 taagtttcca cgcttaatag ttacatcgtt taagttgagc atagaaaatc cttatcgttt
       61 cgccaataca atccagaaaa agaactgcac accgactaat gccgtaataa taccgaccgg
      121 aaataatgcg cccggcacaa ggctttttga tagcacggat gacatcgata aaaatgccgc
      181 acccactaac attgaaccgg gcaggaaaaa gcgttggtct tcgcctagca acattcgggc
      241 aatatgcggg gcaactaagc caataaagcc gataatgcct acaaagctaa tgcaggtggc
      301 cgtcattaca gacacaatca gcaatgtttt cattcgtaac caagttaagt taatgcctaa
      361 gctcaatgct cgagcttcgc ccaaacgcaa ggcagttaat ttccacgcat ctttgaataa
      421 taataaaata cagactgtag ttacgccaaa ggcaattgag atagtctgcc aagtagcttt
      481 agttaaatta ccgaatagcc aaaacagaat ttgttgggat acctcaggtg cagaaataaa
      541 ttgcaccaat gacagtaaag actgaaataa aaacaataag gcaattccta ccagcactaa
      601 tattgccgag ctaaaacgtc ctaaagagga aaaggcaaat aaaatggcgg aagaaaatag
      661 tgtcatcaca aaagccccga caggtacaga aatttcggtg ggtaaaccaa atccaccaaa
      721 ggcaatcaca atcgaagcac caaagccggc tgcagctgct aaacccaatg tataagggct
      781 tgccattgga ttattaagca aagtttgaat ttcagctccg ccaacaccaa gtgctgctcc
      841 aaccaccaat gccattattg ccattggtaa gcgtagctca tagacaatca ctttggtggt
      901 ttcttcaatt tcgcctaatt ggaataaggc tttccaaact tcattaaacg gaagcaggga
      961 agggcctgtc ataatatcga aaatgaggct gagtaaacaa atgagtaaga aaaagagtaa
     1021 taaaaaactg cgtttctttt caatagttcg gtgcttttta gctatttgtt ggggagagag
     1081 cgttgtttgc ataaaaatgt aatgtttgag aattattatc agacaggata agagaaaaga
     1141 tcgataaatt caagaatatc caagataccg actaaagtat tcaactaagt tctcgagtag
     1201 ccctgtcttt tacagtaaaa aactcctgaa tagcggcttc ttctaaaaga gtataattct
     1261 gccaaatttc ggttacagca tttcttaaaa tctcaccttt cttaactctt gatgacattc
     1321 ccaagaatac ctgttctaag aaatctaaat cggcataatg agttaatgct tttcgtgact
     1381 gtaaccagtt aaacatcggc acaaattgtt caggaaaaat cgtgcgttgt tgttcgattt
     1441 gctgaaagat ctgtgcttgt acctcattta aatctttagc aaataaccgt tggaaatgtt
     1501 tagcgagaaa atggtcgatc ataatatccg acacaatgcc tttataacgc ccgaatgtgc
     1561 cttggagtaa ttgggttgtg aggctgtccg gtcggtccga aatcttatca atcgagcggt
     1621 gtaacaccac accgcttttt aagttatccg ctaaattgag atgcggaatt aaacctttat
     1681 agaaatcacc ggcaaagttg ccatataggg tctgttgctt ggtttgggtg tcaatttcaa
     1741 gtgaaatcag cgaatgggta agaaaattca tttatgctcc aagtgcagtt tggtgatact
     1801 caaacccaaa ctcgttggtt tcgcctttcg gtacaaactc aaataaatgc gtaatgcaat
     1861 ttggagcatc gctgtcttgg tgcgaaacaa aaagtagttg cgtttggctg ttagtcacta
     1921 attgttcaat gaactgtttg actaatttgc ggttaatgcc gtctaaacct tgtaacggtt
     1981 cgtctagaat cagaattgat gggtgtttca ccattgctcg ggtaatcaaa agtaagcgtt
     2041 gttgccccca cgagagagag cgaaacggct ttttggcaac attgcttaaa ttgaggcgtt
     2101 ccaaccattc cattgctttt aattgcaagg cattcggcac ttgttgatac acgccgatgc
     2161 tgtcaaaaaa gccagaaaga atgacctcca ttgcggagca atttacccga taatccatat
     2221 gcaactggct gctgacatag ccgatatttt tcttaatatc ccaaatggtt tcgccacttc
     2281 cccgttgttt gccaaatagt cggactttat tggcataaga ttgtgggtga tcgccggtga
     2341 taatcgaaag caaggtcgat ttcccagctc cattcgggcc tttaatccac cagtgttgtt
     2401 taggctgaac cgtccaactt aggttatcta aaatcttctt ctccccatat tgaatattca
     2461 cattttccaa ctcaaacgga ttgatgtttt ccggcaggct gagtagcgga gctgcactat
     2521 caggcagtgg cacattgaca ttctcttctg caaattttag ctgagaatag accgcttgtt
     2581 gttcaatttc tgctcgctct ccttgcagaa ttaatgataa attatctaat aactcaatat
     2641 gattggcagc atcaggaata tcgttaaaac gatttacaat cagcactaac gccattttgc
     2701 tttgtagttc ggcaatcagt tgttgccaat attgcaccga ctgctgatcc agcccttcaa
     2761 acggttcgtc taaaatgaat aaatccggct cgctcactaa catttggcaa aatagcactt
     2821 tgcggctttc accggtggaa aggtgcataa acgggcgttc aagcagatgt tgaatatgca
     2881 atttgtgggc atattcctca cataacgcct cttttggact gccatttaaa attacttgtc
     2941 gtgcggttaa gccgaaatca tcgggcgaga cactatcgtt gttccgatct ttgaaaatct
     3001 gctccaacat tttttgttgt tgctcgaaag agaggagctg aatatgttgg aacgaattac
     3061 catagtcgcc ggaatagagc gagattttac cctgtaaagc gagtgaaaaa gttgttttgc
     3121 ctgaaccgtt tccgtctacg accacctaaa agtcattggg ttggatctgt aaggcgtcaa
     3181 tcgtgagttt atgttgttta accagtgaaa atcgggcatt ggttattgta agtgaaggct
     3241 gtgtttgcat atatttcccg ttttaactta gttgtgtccc gctgacgaac caatccgctc
     3301 gaccgtttaa gaaatcttgc accgacattg gttttttacc cgccggttga agttgggtaa
     3361 tattcagcac gccctgttgg gtggcaattt gaatcccggt tttatccaca ttaattaccg
     3421 tgccggctgc tttttcttgg tgaggtaaca ccgtggcttg gtgaattttg atactttgct
     3481 caacgccatc aacggttaag gttaaaaaac taaacggcca agggttaaaa gctcggatat
     3541 tgcgttccag ttgaactgct gggagttgcc aatccaattt agcttcttct ttggaaagtt
     3601 tctcggcgta attggacagt gcttcatctt gtttttccgc tttgaatgtg tggttggcta
     3661 aaccgtttaa cacctcaagc aatgccagag gggcaagttc tgccagtttg gcatacaagg
     3721 aggcggaggt ttcattcggc tcaatcgggg ttgtgacttt atgtaacata tcaccggtgt
     3781 ctaagccaac gtccattagc ataatggtta cgccggtttc tgtatcacct gcccaaatcg
     3841 aacgctgaat cggagctgct ccacgccagc gaggtagtag cgaaccgtgt acgtttaggc
     3901 aaccatattt aggagcattc aatactgctt caggtagaat taagccgtaa gctaccacca
     3961 ccatcacatc ggcatttagg gctttcagtt cagcttgagc ctcttcctta cgcagggatt
     4021 tgggttggta aacaggaatg ttgtgagctt cggccagctg ttttaccggg ctggcttgca
     4081 gttttttgcc tcgcccggca ggtttatccg gctgagtata aaccgcaatc acgttatgct
     4141 cggaatctaa taaggcttgt aaatgttgag cggcaaaatc aggcgtgccg gcaaaaataa
     4201 tattgagttt tgacatattt tttattcgtt caagttatca atgaaagact tattaaacca
     4261 ttaaacagca caaataaaaa gtggcttgaa tatggtatca ttgccacatt caagataaga
     4321 gaatcaaaca atggaatttt ttatccccaa agcggataca atttttgaag aagagattaa
     4381 aaaaagccga tttatcacct accttcgcca tactgagggt atggagcagg ccaaggcatt
     4441 ttggcaggag ctgaaaattc agcatccgca cgcacgccat tggtgttggg caacggtggc
     4501 aggcttgccg aatgattcgc aacaatacgg cttttcggat gatggagaac cgtctggtac
     4561 agcaggtaag ccaatgctca attatttgct tgggtcaggc ttgggggaaa ttacagcggt
     4621 ggtggttcgc tactacggtg gtatccagct tggcaccggc ggcttggtga aagcctatgg
     4681 caatggggtg caacaggcgt tattgcagtt ggagcggatt cgcaaagttt tgcgaaaaaa
     4741 ctaccgcttg caatgtgaat atgagcagtt taataccatt tctcatctca ttagcgagaa
     4801 agatattgtg attgtcgagc aactttttag cgataaagtg gatttgttgc tggcatttaa
     4861 cccgagcgag gttgaggcct ttgcagatga attaacccaa cgcttttcgg gcaagctcgc
     4921 attaataaaa gaagaaatct agtctatgca atttttttcc attattcgta ttgttggcat
     4981 tttggtgatg tgtttttcat tcacaatgct tgtgccggca acggtcgccc taatttatgg
     5041 cgatggcggt gggcgggcgt ttttagaagc cttcgctctg aattttatgg tcggtacgct
     5101 gctttggtgg ctgtgccgtg acaacaaaaa cgagctacgt tcccgtgagg gctttttgat
     5161 tgtagtactg ttttgggtgg tgctgggttt gctcggggct gtgcctttta ttattttgga
     5221 aaaccccgat ttaaacgtgg cggaatccat ttttgaatcc ttttccggct taactacaac
     5281 gggtgcaacc gtcatcaccg gcttagatag cttgcccaaa gcgattttat tctaccgcca
     5341 atttttgcaa tggctagggg gaatggggat cattgtgtta gcggttgcga ttattccgct
     5401 gttaggcatt ggtggaatgc aactttatcg tgcggaaatg cccggaccgc tcaaagagca
     5461 gaaaatgcga cctcgcattg cggaaacggc aaaagcgttg tggctgattt atctcagcct
     5521 gacgattctg tgtgcggtcg cattttggct cgccggaatg acggtgtttg atgccatttc
     5581 gcatagcttt tccacggttt cgattggcgg attttctacc cacgatgcca gcatcggcta
     5641 ttttaataat gcggcaatta gctatattac cgtgtttttc ctgctacttt ctgcctgcaa
     5701 ctttgcattg cattttgcgg tgattgatcg catacgagat gcacaatcac gcaaaaaagg
     5761 gggtatttta agccgcttgt attggggcga ttacgagttt cgttttttct tgatggtgca
     5821 gttagggctt ttcttggttt gtaccttggt gttagtgggt tacggttatt tcgataatac
     5881 ctctgttacg attgagcaag cgttgtttca atcggtgtcc atttccacca cggccggctt
     5941 tactaccaat gattttagcc aatggagttc gtttttaccc acgctgttgg tgctgtcttc
     6001 atttatcggt ggttgtgccg gctcaaccgg tggcggcttg aaagtgattc gggtgttgct
     6061 gctttattta caaagtaaac gtgaaatcca ccgctacatt caccccaatg tgattcaacc
     6121 gattaaatta ggcaaccacg ttttatcgga acgggtggtt gatggtattt gggcgttttt
     6181 ctcggcgtac tttttcgtgt tcgtgatctg ctggcttgct accatcgctt gtggaatgga
     6241 aacctttgat gctctaaatg cggtaattgc ctcactaaat aatctaggtc ctgcattagg
     6301 gagcgtgagc agcaatttca cccaagtgcc ggacagtgcc aagttagtgc tgacttttgc
     6361 aatggtatgc ggacgtctgg aagtgtttac cttgctggtg attttaagcc ctgttttttg
     6421 gaaagattaa atgaaaacct taattctcta ttttacgacc gatggtcaaa ccaaaaaaat
     6481 tgccgaacga ttggcggacg tgattactca cgaagtcgag ctgatttcgc taaaagatca
     6541 agcggtcgat tttgccgaaa aaattgcaaa tgccgaccaa attgtcatcg gtgcttcaat
     6601 tcgctatggg cattttaacc cgttggttta tcagtttgtg gaacagcatt atccggcatt
     6661 aaacgccaag aaaactgcct tttacggtgt gaatttgacc gctcgcaaag ataatcgaaa
     6721 cacgcctgaa accaatactt atgtgcgtaa atttttagct aaagtgaaat ggaaaccaac
     6781 ccacattgag gtaattgctg gggcattact ttacctacgc tataagccat tcgaccgatt
     6841 gatgattcgc tttattatga aaattaccaa aggcgaaacc gatacctcca aagaatatga
     6901 gtacaccgat tggcagcagg tggagaattt tggcaaatta ttagccaatc agttataatc
     6961 aaactgatct aaatcaaaaa aatgtaaatt tatttaaatg ttgtagaaat tttaaagatt
     7021 ttgattataa tcgccagccc acgttcttgc gaagaccgtg tttaatcatt gaatgaaagg
     7081 aaagccccct tgagtatttt aaaagagttc cgtgaatttg cggtaaaagg taatgttgtt
     7141 gatatggctg tcggtgtgat tatcggcggt gcattcggta aaattgtttc ttcattagtt
     7201 agcgatgttg taatgccacc tatcggttgg ttaatcggtg gcgtggattt caaagattta
     7261 gccattgaaa tcgccccggc aaaagaaggt gcggaagcag tgatgttaaa atacggtgca
     7321 tttatccaaa acgtatttga tttcttaatt atcgcacttg cggtattcgg tatggtgaaa
     7381 gtgattaact cactgaaaaa agctccagtt gtagaagaag ctcctgcaga gccaacagcg
     7441 gaagagaaat tattaactga aatccgtgat ttattgaaaa aataattaac tgattttgaa
     7501 acaagggcta ttttagaata tctagaatag ccctttgtgt tatggttaaa aagaaaaaaa
     7561 ccaacataaa gtcggttttt ttcatatctg ttcgatatta cgctaatttt ttgatttgcg
     7621 ctgctaattt agatttgtgg ttagccgctt tgttagcgtg gattaagttt ttagatgcca
     7681 tacggtctac aactttttgc atttcaacga aagccgtttg agctgctgct ttatcgcctg
     7741 ctgctactgc tgcatatact tttttgatga atgtacgcat cattgagcgt tggcttgcgt
     7801 tgtgttggcg gcgtttttca gattgaaccg cacgtttttt tgctgacttg atattagcca
     7861 aggtcaaact cctaaaatat ctgtgtaaaa attagatata aaca
//
LOCUS       DANLYI010000108         7824 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.108, whole genome shotgun sequence.
ACCESSION   DANLYI010000108 DANLYI010000000
VERSION     DANLYI010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 7824)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7824
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.108"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            <1..4192
                     /locus_tag="PXD81_001644"
     CDS             <1..4192
                     /locus_tag="PXD81_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HDL5537656.1"
                     /translation="TKTFTRSYLASFVTIVLSLPAVASVVRNDVDYQYFRDFAENKGP
                     FSVGSMNIDIKDNNGQLVGTMLHNLPMVDFSAMVRGGYSTLIAPQYLVSVAHNTGYKN
                     VQFGAAGYNPDSHHYTYKIVDRNDYEKVQGGLHPDYHTPRLNKLVTEVVPAAVTNAGT
                     SIKPYLNEERFPMFLRAGSGTQALRGKESNKTTGIAGAYEYLTGGTTLQLSKSSPDHW
                     LDYSSNLYQVSYGPLSTYALPGDSGSGSYAYDMNEKRWVLVGVLNFYNGMDNQFNRSA
                     IIRKDFHEKKFAEDIAGTINNTVQNAQFNWTAQGKSSSLSQSNNVQKLNVDLKDSSIA
                     NQNTSLPQENHGKTINFNGKDATIVLKQDIDQGAGALNLNANLTIRPETDQTWQGAGI
                     IVGKDKKVNWQVKNPQGDRLSKLGEGTLYVNGRGQNLGDISVGDGIVILNQQADHQGR
                     KQAFNTVGIVSGRPTVVLGSADQVNPDNIYFGFRGGRLDLNGNSIAFKRIQNSDKHAR
                     IVNHNRDHISTLIIQGQDPLTSNDLIWGKWASNSPADIYEYTNPYQNKRKDYFRLKGN
                     SRVYYPTNATSNDHWEFLSSNREQAIQKILDAKNLRQRYDTFNGFIGEDASNKTNGIL
                     NVVFDTKTEVNTEQDKLKNIYTMSGGFNLNGELTLKGGTLLLSGHPTPHAYDIKNKHD
                     VVRENDWQDSHFTAKNITVNKMAQLYIGRNVNEVNSHFTATDKAKLNLGFINRSTPSC
                     YDSEYTGTTHCEVQAVISDNIFANLATTAIKGNVKLQNHSQLNLGKANLTGSVQADQT
                     THITLANHSHWLNNGTSQIGHLTMEKGSILSLNDKFATTEIPVRFNKMIIQGNLKGNG
                     RINYTANLAKGESDHLQVDGIAEGNFVLAVRNSTTEANPKSSLNLLSLKNSNQEGNKA
                     SISLENNYVDLGTYRYVLENRNHNYHLFNPLIPNSTSKEMNATSVSSIPKKESVTNVP
                     TLDKKETEQNLTQLQKDFSAHQLENQKAKQSMINAQSELRRLNSQLNVLQKYVNSRRL
                     GYYTQQAVLEQISIIQNKIKQTQTIFNDANATVKLTDQKLEEAKLALGSVNDLVLIKA
                     SAPAMQATNQDTSMMNIIQADWISQYANTALSELSAQANSALQISNSLDRQLFKQSDK
                     FNVWSSVEHQKTEHKSDLYRPYKQQTNLTQLGIQMPIDNGLMFGVALSKNHANAEFNE
                     GVNGKSNLLMASLYGKWQSQQGTFISLDGSYGKAKNQLYLFGENHFTRRISSIGANIG
                     HQFDLAGVQIQPTIGARYYHFSGQDYTLGGAKISSPNTHFMTYQAGLKASKTFSLDDW
                     KVEPSITTHYVDASNKRLAVNVNNNNINQRFGRYLKTEAGIRFEHNQWQFDLHAGFIN
                     GNEIKKQHFAGVKLGYSW"
     gene            complement(4247..5341)
                     /gene="mobA"
                     /locus_tag="PXD81_001645"
     CDS             complement(4247..5341)
                     /gene="mobA"
                     /locus_tag="PXD81_001645"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HDL5537657.1"
                     /translation="MSKTIEERISAVILAGGLARRMNGVEKGLQLFEGKPLISHILAR
                     LSPQVSDIWLNVNRSIEQYQQLYPAFSYYQDSLPDFQGPLSGMLAGFEQIESDYLLFV
                     PCDTPFMPELLLQKLKTALRINNVQIAYAHDGERPHPTFALIHRSVQEDLKAYLGSNQ
                     QQLLAFFQSQKSVAVDFSEQKSAFTNVNTLEDLSRVSQFPVKNLAITGYSGTGKTTLL
                     EKLIPKLTACGIRVGLIKHSHHNVDVDKKGKDSYRLREAGANPTMIVCDERWALMTET
                     KQAVEFSQLIAKFNPQEVDLVFVEGFKHETLPKIQLHRKGIEKPLPELDQWTIATATD
                     YPLERENWLDINNIGGIADFIKNWLENKAS"
     gene            5428..5697
                     /locus_tag="PXD81_001646"
     CDS             5428..5697
                     /locus_tag="PXD81_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1040 family protein"
                     /protein_id="HDL5537658.1"
                     /translation="MKCERLEELLELLGEHWRKNPDLHLIDILQQLSVEVGEPDNFKA
                     LSDEVLIYQLKMRNAGKFEPIPGIKKDYEDDFKTALLRARGILND"
     gene            5761..6399
                     /locus_tag="PXD81_001647"
     CDS             5761..6399
                     /locus_tag="PXD81_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005602749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HDL5537659.1"
                     /translation="MKKLALKTAFVALSALFTFNSTAIAADPTAGKEYIEVRKAPSAQ
                     KEVVEFFSFYCPHCYDFELSYKIPSQIKEKLPSDSKLVQYHVNFLGRQSEDLTRAWAL
                     AMALGAEDKVKTALFEGAQKDAFKSMDDIRSVFLANGITAEQFDSGINSFAVNGLVNK
                     QVQLAEDFQIRGVPAFFVNGQYQLNLEGFADSSSTNDFIKRYVDAVVFLSKK"
     gene            6483..7259
                     /locus_tag="PXD81_001648"
     CDS             6483..7259
                     /locus_tag="PXD81_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HDL5537660.1"
                     /translation="MTKLSILNLVPIREGQTAKQAVDSMVKLAKHAESIGIERYWIAE
                     HHNMKNLASSATQLLIQHALSHTEKLRVGSGGVMLPNHSPYIVAEQYGTLETLYPNRV
                     ELGLGRAPGTDMRTATALRRNAQSLPFPDEISELKGYFEGTNSVSAYPAQGLNVPFYI
                     LGSSPESAYLAAERGLPYAFASHFAPRFMDDAVRIYRTHFKPSEVLDKPYVILGVNAI
                     AAETDQEAEQLATTQTQFFLNVVTNAQNYLQPPVESDDKV"
     gene            7269..7523
                     /locus_tag="PXD81_001649"
     CDS             7269..7523
                     /locus_tag="PXD81_001649"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="luciferase"
                     /protein_id="HDL5537661.1"
                     /translation="MKAEIVPHFGPVSFQQNALYNQEKPVVRQMTACSLIGSRESIEQ
                     QLKSLKARIEIDEIMAVSYIFDEQKQHQSYTWLKEIADKV"
     gene            7525..7818
                     /locus_tag="PXD81_001650"
     CDS             7525..7818
                     /locus_tag="PXD81_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005598606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HDL5537662.1"
                     /translation="MSKTMQQKAADAHNMCKLKGDSMLDNSDRQIAFEAVYDSEEIAV
                     QAVEFFTQKAKSVETEPCDIQSEITPIIDGFLMKMWITFSCQAEVILFQMAVR"
BASE COUNT         2612 a         1552 c         1476 g         2184 t
ORIGIN      
        1 gaccaaaaca tttactcgtt cttatcttgc ttcttttgta acaatcgtat taagtttacc
       61 tgctgtagca tctgttgtac gtaatgatgt ggactatcaa tacttccgcg attttgccga
      121 aaataaagga ccattttcag ttggttcaat gaatattgat attaaagaca acaatggaca
      181 acttgtaggc acgatgcttc ataatttacc aatggttgat tttagtgcta tggtaagagg
      241 tggatattct actttaattg caccacaata tttagttagt gttgcacata atactggata
      301 taaaaatgtt caatttggtg ctgcaggtta taaccctgat tcacatcact atacttataa
      361 aattgttgac cgcaatgatt atgaaaaggt tcaaggaggg ttgcacccag actatcatac
      421 tcctcgatta aataaattag taacagaagt tgtgcctgcc gcagtcacca atgcaggtac
      481 atctattaaa ccctacttaa atgaagaacg cttccctatg tttcttcgtg ctggttcagg
      541 gacacaagcg ctaagaggaa aagaaagtaa taaaacaact ggaatcgctg gtgcttatga
      601 atatcttact ggcggtacca cattacaatt atctaaaagc tcccctgatc actggttaga
      661 ttattcaagt aacctttatc aagtaagcta tggaccactt tcaacctatg cactacctgg
      721 tgatagtggt tcaggttctt acgcctatga tatgaacgaa aaacgatggg tattagttgg
      781 tgtgctcaat ttctataatg gtatggataa tcaattcaac cgctctgcga ttatccgtaa
      841 agatttccac gagaaaaaat ttgccgaaga tattgcagga acaatcaata ataccgtaca
      901 aaatgcacaa ttcaattgga ctgctcaagg taaatccagc tctcttagtc aatcaaataa
      961 tgtgcaaaaa ctcaacgttg atctaaaaga tagtagcatt gcaaaccaaa acacttctct
     1021 gccacaagaa aatcacggta aaaccattaa ttttaatggt aaagatgcaa ctattgtact
     1081 aaaacaggat attgaccaag gtgcaggtgc attaaatctg aacgctaatc tcactattcg
     1141 tcctgaaaca gaccaaactt ggcaaggtgc aggtattatc gtcggtaaag ataaaaaagt
     1201 gaattggcaa gtaaaaaatc cacaaggcga tcgtttatct aaactcgggg aaggaacact
     1261 ctatgtaaat ggacgtggac agaatcttgg cgatatcagt gtaggtgatg gtattgtaat
     1321 acttaaccag caagccgatc accaaggaag aaaacaggcc tttaatacag taggaatcgt
     1381 aagtggtcgc cccactgttg tgctaggtag tgcagatcaa gttaatcccg ataatattta
     1441 ctttggattt cgcggaggtc gtttagacct aaacggtaac agcatcgcct ttaaacgtat
     1501 tcaaaacagc gataaacatg ctcgtattgt aaaccacaat cgcgatcaca tttctacctt
     1561 aataatacaa ggccaagatc ctctcactag taatgatctt atatggggaa aatgggcaag
     1621 taatagccca gcagacattt acgaatatac caatccttat caaaataaac gcaaagatta
     1681 cttccgtctg aaaggtaatt cgagagtata ttatccaacg aatgctacaa gtaacgatca
     1741 ctgggaattt ctttccagta accgcgagca agcaatacag aaaatcctag atgccaaaaa
     1801 cttaagacag cgctatgaca cgtttaatgg ttttataggg gaagatgctt ccaataaaac
     1861 taatgggata ttaaatgtcg tgtttgatac aaaaacagaa gtaaatacag aacaagataa
     1921 attaaagaat atctacacaa tgtcgggagg atttaacctt aatggtgaac tcacccttaa
     1981 aggtggtaca ttgttgcttt ctggtcaccc aacgccacac gcttatgata ttaagaataa
     2041 gcatgatgtt gtgcgtgaaa acgattggca agacagccat tttactgcta aaaatatcac
     2101 ggtaaataaa atggcacaac tctatatcgg gagaaatgtc aatgaagtaa atagtcactt
     2161 tactgcgact gataaagcca aactcaattt aggatttatt aatcgttcaa cgccaagttg
     2221 ctatgattct gaatacacag gcactacaca ttgtgaagtg caagcggtca tttccgataa
     2281 tatttttgca aatctagcaa caaccgccat taaaggtaat gttaaattac aaaaccatag
     2341 ccaattaaat ttaggcaaag caaacctcac tggttctgta caagctgatc aaacaactca
     2401 tatcacttta gcaaatcaca gtcactggtt aaacaatggt acgagccaga ttgggcatct
     2461 tacaatggaa aaagggtcga tccttagcct aaacgataaa tttgctacca cggaaatccc
     2521 agtccgattc aacaagatga tcatccaagg taatctaaaa ggtaatggac gaattaacta
     2581 taccgcaaat ttagccaagg gcgaatctga tcatctccaa gttgacggta ttgctgaagg
     2641 aaattttgtc cttgccgtta gaaatagcac aactgaagca aatccaaaaa gctcattaaa
     2701 cctactaagc ttaaaaaata gcaaccaaga aggcaataaa gcttctattt ctctagaaaa
     2761 taattatgtt gatctaggta cttatcgtta tgtattagaa aatcgtaatc acaattacca
     2821 tttatttaat ccattaatac caaattcaac ctctaaagag atgaatgcta catctgtatc
     2881 ctctattcca aaaaaggaat ctgttactaa tgttcctact ttagataaga aagaaactga
     2941 acaaaatctt actcaactac aaaaagattt ttcagcacac caattagaaa atcaaaaagc
     3001 aaaacaatct atgataaatg ctcaatctga gctaagacga ctcaattcac aactgaatgt
     3061 attgcaaaaa tatgtgaatt ctcgtcgctt aggttactat actcagcagg cagttttaga
     3121 acaaattagc attattcaaa ataaaattaa acaaacacaa acaatattta atgacgctaa
     3181 tgcaactgta aaactcacag atcaaaagct agaagaagcc aaattagctc taggctctgt
     3241 aaacgatctt gtattaataa aagcctctgc tccagcaatg caagcaacta atcaagatac
     3301 gagtatgatg aatattattc aagcagattg gataagccaa tacgctaaca cagcactttc
     3361 tgaactctcg gcacaggcta attctgctct gcaaatcagt aatagcttag atcgccaact
     3421 cttcaaacaa agcgataaat tcaacgtatg gagcagcgtc gaacatcaga aaaccgagca
     3481 taaatcagat ttataccgcc cgtataaaca acaaaccaac ctgacccaac tgggcataca
     3541 aatgccgata gataacggtt taatgtttgg agttgcatta tctaaaaacc acgctaacgc
     3601 ggaatttaac gagggtgtaa acggtaaatc gaatctacta atggcaagcc tatatggtaa
     3661 gtggcaatct caacaaggca cttttatcag ccttgatggc agctacggta aagcaaaaaa
     3721 ccaactctac ctatttggtg aaaaccactt tacccgccga atttcctcta ttggtgctaa
     3781 cattggacat caatttgacc tcgcaggagt tcaaattcag ccaacaatag gagcaagata
     3841 ctaccatttc agcggccaag actatacact aggaggagcg aaaatcagct caccaaatac
     3901 ccactttatg acatatcaag cgggtctaaa agctagtaaa accttttcat tggatgactg
     3961 gaaagttgaa ccaagcatta caacccacta tgtggatgca agtaacaaac gcttagcagt
     4021 aaacgtcaat aataacaata tcaaccaacg tttcggtcgc tatctgaaaa ccgaagctgg
     4081 tatccgattt gaacataacc aatggcaatt cgacttacac gctggtttca ttaacggcaa
     4141 cgaaatcaaa aaacaacatt ttgctggtgt taagctagga tatagctggt aagaaaaagc
     4201 ccctagaact atttctaggg gctttttcta ctcttaaaaa gagtatctaa cttgccttat
     4261 tttctaacca attcttaata aaatctgcta ttcccccaat attattaata tccaaccaat
     4321 tttctctctc aagcgggtaa tccgttgcgg tggcaatcgt ccattggtct agctctggca
     4381 atggtttttc aatccctttg cggtggagct gaatttttgg caaggtttcg tgtttaaagc
     4441 cttctacgaa aacgagatca acctcttgcg ggttgaattt tgcaattaat tgagaaaatt
     4501 cgaccgcttg tttggtttct gtcattaatg cccaacgttc atcgcacaca atcattgtcg
     4561 ggtttgctcc tgcttcacgt aggcggtagc tgtctttgcc ttttttatct acatcaacgt
     4621 tatggtggga atgtttaatc agcccaaccc gaataccgca agcggtcaat tttgggatta
     4681 atttttccaa taaggtggtt ttacctgtgc cgctgtagcc tgtaattgca aggtttttta
     4741 cagggaattg gcttactcgg cttaaatctt ccaatgtatt cacgttggtg aatgccgatt
     4801 tttgttcgga aaaatcaact gccacgcttt tttgtgattg gaagaacgca agcaattgct
     4861 gttggtttga gcctaaatag gctttcaaat cttcttgtac cgaacgatga atcaacgcaa
     4921 aagtgggatg tggacgctca ccatcatggg catatgcaat ttgtacgtta tttatccgca
     4981 aagcagtttt taatttttgc aataacagct caggcataaa gggcgtatcg caaggcacaa
     5041 aaagcaaata gtcactttca atttgctcaa atcctgccag cattccactg agtggacctt
     5101 gaaaatcagg caagctatct tgatagtaag aaaaagcagg ataaagctgc tgatattgct
     5161 caatagaacg attgacatta agccaaatgt cactaacttg tggtgaaagt cgggctaaaa
     5221 tgtgtgaaat aagcggtttt ccttcaaaaa gttgtaaccc tttttctacc ccattcattc
     5281 gacgagctaa accgcccgct aaaatcacgg ctgaaatgcg ttcttctatt gttttactca
     5341 cagctttatc ccttaaaatt ttgcaaaaat aagagtaaaa gtatattatt tcgtttaatt
     5401 tccaacaagt tttttaaggg gtaaaagatg aaatgcgaac gtttagaaga gttattagag
     5461 ttacttggcg aacattggcg taaaaatcct gacttacacc tcattgatat tttgcagcag
     5521 ctttcagttg aagtgggcga gcctgataat ttcaaagcgt taagcgatga agtgttaatc
     5581 tatcagctta aaatgcgaaa tgcaggcaaa tttgagccta ttcccggcat aaaaaaagat
     5641 tatgaagatg attttaaaac ggctttattg cgagctcgtg gaattttaaa cgattaagat
     5701 tgtcttatcc gttagctagt ttagcattta ttatttatca acatttttga aggaatcatt
     5761 atgaaaaaat tggcattaaa aacggcattt gttgcacttt ctgccctttt tacatttaac
     5821 tcaacggcaa tcgctgctga tccaaccgca ggcaaagaat atatcgaagt gcgtaaagca
     5881 ccttctgcac aaaaagaagt ggtggaattt ttctcattct actgcccaca ctgttatgat
     5941 tttgaattaa gctacaaaat cccaagccaa atcaaagaaa aattaccaag cgatagcaaa
     6001 ttagtgcaat atcacgttaa tttcttaggt cgccaatctg aagatttaac tcgtgcttgg
     6061 gcattagcaa tggcattagg tgcggaagat aaagtaaaaa ccgcattatt tgaaggtgct
     6121 caaaaagacg cattcaaatc aatggacgat attcgctctg tattcttagc aaacggcatt
     6181 acagctgaac agtttgatag cggtattaat agctttgcgg taaatggctt agtgaataaa
     6241 caagttcaat tagcggaaga cttccaaatt cgtggtgtgc ctgcattctt cgtaaatggt
     6301 caataccaac tcaacttaga aggctttgca gattcttcat caacgaatga tttcatcaaa
     6361 cgttatgttg atgcggttgt gttcttaagc aagaaataac cccttaaaat agttagggtt
     6421 tttacaatgc tgagtaggta aatcctattc agcatttttt atatgcaaac tcggagaaca
     6481 aaatgacaaa gttatctatc ctaaaccttg tacctattcg tgaagggcaa actgccaaac
     6541 aagcggtcga ttctatggtg aaattagcaa aacacgcgga aagtatcggc attgaacgct
     6601 attggattgc agagcaccac aatatgaaaa accttgccag ctcggcaaca cagctcctca
     6661 ttcagcatgc cctttctcac actgaaaaat taagagtggg atcaggtggc gtgatgttgc
     6721 ctaaccatag tccttatatt gtggcagagc aatacggtac gcttgaaaca ctctacccta
     6781 accgtgtaga acttgggctt gggcgagcac caggcaccga tatgcgaacc gctaccgctt
     6841 tacgccgtaa tgcacaaagt ctgccatttc ctgatgaaat cagcgaacta aaaggttatt
     6901 ttgaaggcac aaattctgtg tctgcctacc ctgcacaggg gttgaatgtg ccgttttata
     6961 ttttaggctc aagccctgaa agtgcctatt tagcagcaga acgtggcttg ccttatgcct
     7021 ttgcttcaca tttcgctcct cgttttatgg acgatgcggt gcgaatttat cgcacacact
     7081 ttaagccgtc tgaagtgtta gataaaccct atgtgatttt aggtgtgaat gcgattgcgg
     7141 cagaaaccga ccaagaggca gaacagcttg ctaccactca gactcagttt ttcttaaatg
     7201 ttgttaccaa tgcccaaaac tatttacaac cgcccgtcga atccgatgat aaagtgtgaa
     7261 aaaattttgt gaaagcggaa atcgtaccgc atttcgggcc tgtttcgttc caacaaaatg
     7321 cgttatacaa ccaagagaaa cccgttgttc gccaaatgac tgcttgttca cttatcggta
     7381 gccgagagag cattgaacaa cagctcaagt cattaaaagc acgcattgaa attgatgaaa
     7441 ttatggcggt aagctacatt ttcgatgagc aaaaacagca tcaatcctat acttggttaa
     7501 aagagattgc agataaggtg tgatatgagc aaaacaatgc aacaaaaagc cgctgatgca
     7561 cataatatgt gtaagctaaa aggcgactct atgttagata actcagaccg ccaaattgcc
     7621 tttgaagcag tgtatgatag tgaagaaata gctgtacaag cggtcgaatt tttcacacaa
     7681 aaagcaaaat cggtagaaac cgagccttgc gacattcaaa gtgaaatcac acccattata
     7741 gatggctttc tgatgaaaat gtggataact ttcagttgcc aagcagaagt aatcttattt
     7801 caaatggcgg tgagatagat ataa
//
LOCUS       DANLYI010000109         7731 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.109, whole genome shotgun sequence.
ACCESSION   DANLYI010000109 DANLYI010000000
VERSION     DANLYI010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 7731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7731
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.109"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            46..3117
                     /gene="fdnG"
                     /locus_tag="PXD81_001651"
     CDS             46..3117
                     /gene="fdnG"
                     /locus_tag="PXD81_001651"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014550636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:631..633,aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HDL5537663.1"
                     /translation="MQISRRKFFKVCAGGMAGTSAAMLGFAPATALAAPREYKLLRAK
                     ETRQTCTYCAVGCGMIMYSLGGEAMNSKGKLIHIEGDSDHPVSRGALCPKGAGALDYV
                     NSDRRVHYPEVREAGSSEWKRISWHEAIERIARHMKNDRDENFVTHNEAGLEVNRWMT
                     AGFLAGSACSNETGIVTQKFIRSLGMIFTDNQASIUHGPTVASLAPSFGRGAMTNHWV
                     DIKNSDLVIVMGGNAAEAHPVGFRWAVEAKKQNGAKLMVVDPRFNRSAAVADIYIPLR
                     PGTDIAFLLGAVNYLLKNDKIQHEYVKHYTNATFLLNEGFKFEDGLFTGYDEATRTYK
                     DKSTWAYQLDEQGQPKRDMTMQDPRCVINMLKDHVSRYTPEMVERITGTPQKDFLIFC
                     EEIAKTSAPDKAATFLYALGWTQHTVGAQNIRSMAIIQLLCGNIGVSGGGVNALRGHS
                     NVQGITDLGLFPHMLPGYIRLPTEADATLESYLNAIAPKTLVAGQVNYGQNNPKFAVS
                     MLKTFYGDKATKENEFGYHYLPKYPKGGTDHFRFIEQMYQGKVNGYFCQGMNPIASYP
                     NSQKLIKALSNLKYLVIFDPLITDTSEFWKNHGEFNNVNPSEIQTEVFRLPTTCFAEE
                     DGSIANSGRWLQWHWKAADAPHEAKPDVDILSEIRAELIHLYHKEGGKAPLEPLEAMT
                     WNYMNPLEPKPEEIAKENNGYALEDLKDADGNIIVKKGELLSSFTQMRDDGTTSGGCW
                     IYCGQWTPKGNQMANRDNSDPSGLGNTLGWAFSWPVNRRILYNRASADLSGKPWNPKR
                     QLVKWNGKNWNYVDVADYGTAPPNSPVTPFIMQPEGVSGLFVRERMQDGPFPEHYEPF
                     ETPIGTNPLHPNVISSPVARILPSDKEDLGTSDEFPYVGTTYRLTEHFHYWTKNALIN
                     VIAQPEQFVEIGEKLAEEKGIKNGDKVRVWSKRGEIKAVAVVTKRIRSLVSDGKPIHT
                     IGVPIHWGFAATTGAKKGYFANNLTVRTGEPNSNTPESKCMLLNIEKIVGA"
     gene            3120..4034
                     /gene="fdxH"
                     /locus_tag="PXD81_001652"
     CDS             3120..4034
                     /gene="fdxH"
                     /locus_tag="PXD81_001652"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit beta"
                     /protein_id="HDL5537664.1"
                     /translation="MSTVQTQNIIKISATSALTPAPQARSHQVEVAKLIDVTTCIGCK
                     ACQVGCSEWNDIRSDVNANCVGVYDNPADLNAKAWTVMRFNEVEENDRLEWLIRKDGC
                     MHCAEPGCLKSCPSPGAIIQYANGIVDFQSDKCIGCGYCIAGCPFNIPRMNPDDNRVY
                     KCTLCVDRVNVGQEPACVKTCPTGAIRFGSKEEMKFYAEQRIADLKSRGYENAGLYDP
                     EGVGGTHVMYVLHHADKPELYSGLPKDPKIDTTVTLWKDVLKPVAAVALGGLALAEVA
                     HYITVGPNVEEDLEEHHHSENAEGGKHE"
     gene            4027..4704
                     /locus_tag="PXD81_001653"
     CDS             4027..4704
                     /locus_tag="PXD81_001653"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit gamma"
                     /protein_id="HDL5537665.1"
                     /translation="MSKKFEITNDTKIVRHKPLARVSHWFLVISFFMTMFTGVAFFFP
                     DFAWLTEILGTPQIARAVHPFTGIIMFIAFIIMALIYWHHNIPEKNDIRWAKGIVEVL
                     KGNEHKVSDNGKYNLGQKLLFWTLILAMFTLLATGIIMWRQFFSHYFSIPMLRIAILL
                     HSASAFMLFTGILVHMYMAFWVKGSIRGMVEGWVTVRWAKKHHPRWYREEVLPELERQ
                     VAEQNNK"
     gene            4760..5677
                     /gene="fdhE"
                     /locus_tag="PXD81_001654"
     CDS             4760..5677
                     /gene="fdhE"
                     /locus_tag="PXD81_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005622482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HDL5537666.1"
                     /translation="MSIKILPQDDIKQAASSFQQPELLFANPKNLYLRRAKRLRELAK
                     QNPFGEYMEFSANLVDIQLALLESQPIADYSQKITACVERTQGETPLNAQNFQRTDEW
                     RTLLLAIIEKFKPYANDTVLATIEVLEKAAKSELDTLADNLLNERYELVGADKAVFIW
                     AALSLYWVQLAQQLPRNSRAEIGHKHLCPVCNSAPVSSVIHFGEAQGLRYLHCSLCES
                     EWHVVRTKCTNCDETGKLDYWSLDTVEAAVKAESCGDCHSYLKVLYQDKDVNVEPVAD
                     DLATLFLDSEMEQKGLSRSGINPFLFQVE"
     gene            complement(5747..6133)
                     /locus_tag="PXD81_001655"
     CDS             complement(5747..6133)
                     /locus_tag="PXD81_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5537667.1"
                     /translation="MLRNFMIMLHFIYGFAMKNAFSFFVISLFLTMNSISYASNPIPS
                     NKEKLNGVKKQQDKPKFRISERKVLKNKEKDEEVSNFSMEELRVGAQRPLDDSVIQYF
                     KSQCRYAFMSDREIIANRCEVKKIAD"
     gene            complement(6144..7514)
                     /gene="gorA"
                     /locus_tag="PXD81_001656"
     CDS             complement(6144..7514)
                     /gene="gorA"
                     /locus_tag="PXD81_001656"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009499791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HDL5537668.1"
                     /translation="MAKHYDYIAIGGGSGGIASINRAASYGQKCAIIEAKHLGGTCVN
                     VGCVPKKVMFYGAQVAEAINHYAPDYGFDITVNNFDYAKLVESRQAYIGRIHTSYGNV
                     LAKNNVDVLNGFARFKDAKTLEVSYADGSVEEVTADHILIATGGRPTVPNVKGAEYGI
                     TSDGVFALDALPKSVAIVGAGYIAVELAGVFNSLGVNTHLFVRQHAPMRNQDPLIVDT
                     LLEVLAQDGIELHTQATPQEVVKNADGSLTLKLQDGRETSVESLVWAIGREPATDKIN
                     LEAAGVETNERGFIKVDKFQNTNVPNIYAVGDIIEGGIELTPVAVAAGRRLSERLFNN
                     KPNEHLDYSLVPTVVFSHPPIGTVGLSEPKAIEQYGAENVKVYKSSFTAMYTAVTQHR
                     QPCRMKLVCVGEGEKVVGLHGIGFGVDEMIQGFAVAIKMGATKADFDNTVAIHPTGSE
                     EFVTMR"
     gene            complement(7591..>7731)
                     /locus_tag="PXD81_001657"
     CDS             complement(7591..>7731)
                     /locus_tag="PXD81_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HDL5537669.1"
                     /translation="LQTFINASNLDEKQMMWQVVIHLAFIVSALAMAYIDKMLNSGHM
                     KH"
BASE COUNT         2310 a         1697 c         1628 g         2096 t
ORIGIN      
        1 atcaaatttt gttttatttc tcacattatg tgatttggag tgattatgca gatctcaaga
       61 cgtaagttct tcaaggtctg tgcaggtggt atggcgggaa cttctgctgc aatgttgggt
      121 tttgctccag caacagcatt agctgcaccg cgtgagtata agctcttaag agcaaaagaa
      181 actcgacaaa cctgtactta ctgcgcggta ggctgtggca tgattatgta cagcttaggc
      241 ggcgaagcaa tgaacagcaa aggaaagtta attcatattg agggtgattc tgaccatcca
      301 gtaagccgtg gggcgttatg tccgaaaggt gcaggggcgt tggactatgt taatagtgac
      361 cgccgtgtgc attatccgga agtgcgtgaa gccggttcaa gtgagtggaa acgcatttct
      421 tggcacgagg caattgaacg tattgcccgc catatgaaaa atgaccgtga tgagaacttc
      481 gtcacccaca atgaagcagg gctggaagta aaccgttgga tgacggcagg tttcttggca
      541 ggctcggcgt gtagtaacga aaccggtatc gtcacccaga agtttatccg ctctctcggt
      601 atgattttta ccgacaacca agcgagtatc tgacacggac caacggtagc aagtcttgct
      661 ccatcatttg gtcgcggtgc gatgaccaac cactgggttg atattaaaaa ctccgattta
      721 gtgatcgtta tgggcggtaa cgcagccgaa gctcaccctg tgggtttccg ttgggcagtt
      781 gaagcgaaaa aacagaacgg tgcgaagtta atggtggtag atccacgctt taaccgtagt
      841 gctgcggtgg cggatattta tataccattg cgtccgggta cggacattgc gttcttgtta
      901 ggtgcggtaa actacctgct taaaaacgac aaaattcaac acgagtatgt gaagcattac
      961 accaatgcca cattcttact caacgaaggg tttaaattcg aagacgggct tttcaccggc
     1021 tacgatgaag ccactcgtac ctataaagac aaatccactt gggcttatca gttagatgaa
     1081 cagggtcagc cgaaacggga tatgacaatg caagatccac gttgtgtgat caatatgctc
     1141 aaagaccacg tttcccgcta tacgcctgaa atggtagaac gcattaccgg tacgccacaa
     1201 aaagatttcc tgattttctg tgaagagatt gcgaaaacct cagcgccgga taaagccgcc
     1261 accttcctct atgccttagg ttggacacaa cacaccgtgg gcgcacaaaa cattcgctca
     1321 atggcgatca ttcagttgct gtgcggtaac attggtgtct ccggcggtgg ggtaaacgca
     1381 ctgcgtggtc actctaatgt acaagggatt accgacctag gcttgttccc acatatgttg
     1441 ccgggctata tccgcctgcc aaccgaagcg gacgcaacct tagaaagcta cttaaatgcg
     1501 attgcaccga aaaccttagt ggcagggcaa gtaaactacg ggcaaaacaa cccgaaattc
     1561 gctgtgagta tgctcaaaac cttctacggc gataaggcaa ccaaagagaa cgagttcggc
     1621 tatcactatt taccgaaata cccgaaaggc ggtacagatc acttccgctt tatcgaacag
     1681 atgtatcaag gcaaagtgaa cggctatttc tgccaaggta tgaacccgat tgcctcttac
     1741 cctaactcgc agaagttaat taaagcgttg agcaatttga aatatttagt catttttgac
     1801 ccgcttatta ccgatacttc cgagttctgg aaaaatcacg gtgagttcaa caatgtgaac
     1861 ccgtctgaaa ttcaaaccga agtgttccgc ttgccgacca cttgctttgc ggaagaagat
     1921 ggttcaattg cgaactccgg tcgctggtta caatggcact ggaaagcagc agacgcacca
     1981 cacgaggcaa aaccggacgt ggatattctg tctgaaatcc gtgccgaact gatccacctt
     2041 tatcataaag aaggcggaaa agcaccgctt gaaccgttag aagcgatgac gtggaactat
     2101 atgaacccgt tagaaccgaa gccggaagag atcgccaaag agaacaacgg ttatgcattg
     2161 gaagatctga aagatgcaga cggcaacatc atcgtgaaaa aaggcgaact attatccagc
     2221 tttacacaaa tgcgtgatga cggtacaacc tccggtggtt gttggatcta ctgcggtcaa
     2281 tggactccga aaggtaacca aatggcgaac cgtgataact ccgacccatc aggcttaggt
     2341 aacaccttag gctgggcatt ctcgtggccg gtaaaccgcc gtattctata caaccgtgca
     2401 tcggcagatt taagcggtaa accgtggaac ccgaaacgcc agctagttaa atggaacggt
     2461 aaaaactgga actatgtgga cgttgccgat tacggtacag caccaccaaa tagccctgtg
     2521 acaccattta ttatgcaacc ggaaggggta tccggcttgt tcgtgcgtga acgtatgcaa
     2581 gacggtccat tccctgagca ttatgaaccg tttgaaacgc caatcggcac gaacccgtta
     2641 cacccgaatg taatcagcag tccggtggca cgcattctgc cgagcgataa agaggattta
     2701 ggcaccagcg atgagttccc atatgtcggt acaacttacc gcttaaccga acacttccac
     2761 tactggacga aaaatgccct gattaacgtc attgctcaac cggagcaatt cgtggaaatc
     2821 ggcgagaaac ttgccgaaga gaaaggcatt aaaaatggcg acaaagtacg tgtttggtct
     2881 aaacgtggcg aaattaaagc cgttgccgtg gtgactaaac gtatccgttc attggtctct
     2941 gacggcaaac caatccacac tatcggtgtg ccgatccact ggggctttgc ggctacaacc
     3001 ggtgcgaaaa aaggctattt tgccaataac ttaaccgtgc gaacaggtga accaaactca
     3061 aatacacctg aatcgaaatg tatgttatta aacattgaga aaattgtggg ggcttaacaa
     3121 tgagtacagt tcaaactcaa aacattatta aaatctccgc aacctcggcg ctgacaccgg
     3181 cgccgcaggc tcgttcgcac caagtggaag tagcgaaact gatcgatgtc accacttgta
     3241 tcggctgtaa agcctgtcaa gtgggctgtt cagaatggaa tgacattcgc tccgatgtaa
     3301 atgcaaactg tgtcggcgtg tacgacaacc ctgcggatct aaatgcgaaa gcgtggacag
     3361 taatgcgctt taacgaagta gaagaaaacg accgtttaga atggttgatc cgtaaagacg
     3421 gctgtatgca ctgtgccgag ccgggttgct tgaaatcttg cccgtcaccg ggtgcgatta
     3481 tccaatacgc caacggtatt gttgatttcc aatccgacaa atgtatcggt tgtggctact
     3541 gtatcgcagg ttgtccgttc aacattcctc gtatgaatcc ggacgataac cgtgtttaca
     3601 aatgcaccct gtgtgtggat cgtgtcaatg ttggtcaaga accggcttgc gtgaaaacct
     3661 gcccgaccgg tgcaatccgc ttcggtagca aagaagagat gaagttctat gcagaacaac
     3721 gcattgctga tctgaaatct cgtggctatg aaaatgccgg cttgtacgac cctgaaggcg
     3781 ttggcggtac gcacgtaatg tatgtattac atcatgccga caaaccggag ctttattcag
     3841 gcttaccgaa agatccgaag attgatacca cagtaacctt atggaaagac gttttaaaac
     3901 ctgtggcggc agtagcgttg ggtggtttag ctttagcaga agttgcacac tatattaccg
     3961 ttgggccaaa cgttgaggaa gatcttgaag aacaccacca tagcgaaaat gcagaaggag
     4021 gcaaacatga gtaagaaatt tgaaatcacg aacgatacca aaatcgttcg tcataagccc
     4081 ttagctcgtg ttagccactg gttcttagta atctcttttt ttatgacgat gtttaccggt
     4141 gttgccttct tcttccctga ctttgcttgg cttaccgaga ttttaggtac tccacagatt
     4201 gcccgtgcag tccacccatt taccgggatt ataatgttta ttgcatttat tattatggcg
     4261 ttaatatatt ggcaccataa tatccctgag aaaaatgata ttcgttgggc aaaaggcatt
     4321 gttgaagtac taaaaggtaa tgagcataag gtttccgata atggaaaata caatttgggt
     4381 caaaaattac tcttttggac tcttattctt gcgatgttca ctcttttagc cacagggatt
     4441 attatgtgga gacaattttt ctctcactac ttctcgattc ctatgctaag aattgcaatt
     4501 ttacttcatt cagcaagtgc atttatgcta tttaccggaa ttttagtaca tatgtatatg
     4561 gcattctggg taaaaggctc aatcagaggt atggttgagg gttgggttac cgttcgctgg
     4621 gcaaaaaaac accacccaag atggtaccgt gaagaagtgc tacctgaatt agaaagacaa
     4681 gtagctgagc aaaataataa ataacctcaa gtgcggtatt attaccgcac ttttatttta
     4741 ttggtttaaa aagaatatta tgagtatcaa aatactacct caagatgata tcaaacaagc
     4801 agctagttca tttcagcaac ccgagctctt atttgctaat cccaaaaatc tgtatttacg
     4861 ccgcgctaag cggctacgtg aattagccaa gcaaaaccca ttcggtgaat atatggagtt
     4921 ttctgctaac ctcgtggaca ttcaattagc attacttgaa agccagccaa ttgcagatta
     4981 ttctcaaaaa ataaccgctt gtgtagagcg cacgcaagga gaaacaccgc taaatgcaca
     5041 gaattttcag cgtactgatg aatggcggac actactctta gccattattg agaaattcaa
     5101 gccttatgca aatgatactg ttcttgcaac aattgaagta ttggaaaagg cagctaagtc
     5161 agagctagac acacttgctg ataatttgct aaatgaacga tacgaacttg tgggcgcaga
     5221 taaagctgtc tttatttggg ctgctctctc actttactgg gtacaattag ctcaacaatt
     5281 accacgtaat tctcgtgcag aaataggcca taaacattta tgtccggtat gtaactcagc
     5341 acctgtaagc agtgtgattc atttcggtga agctcaaggg ttacgttatc tacactgttc
     5401 tttatgtgaa agtgaatggc atgtagtacg tacaaagtgt acaaactgtg atgaaaccgg
     5461 caaactggat tattggagct tagatacagt agaagcagca gttaaagctg aaagttgtgg
     5521 cgactgccat tcctacttaa aagtactcta tcaagataaa gacgtgaatg tggaacctgt
     5581 tgctgatgat ttagcaactc tattcttaga ttccgagatg gaacaaaaag gtctttcaag
     5641 aagtgggata aatccatttt tatttcaagt agagtaactc atttacaaca aaaacggagc
     5701 ataacgctcc gttttttatt acatttaact catacactat acttttttaa tcagctattt
     5761 ttttaacttc acagcggtta gcaataatct cccgatctga cataaatgcg tatcggcatt
     5821 gagatttaaa atattgaatc acagaatcat caagaggtcg ttgtgctcca acacgtaatt
     5881 cctccataga aaaattacta acctcttcat ctttctcttt atttttcaat accttacgtt
     5941 cagatattct aaacttaggc ttgtcttgct gtttttttac tccgtttaat ttttctttat
     6001 tactaggaat aggattactt gcataactaa tactattcat cgtaagaaaa agagaaataa
     6061 caaaaaagct aaaagcgttt ttcatagcaa acccataaat aaaatgtagc ataatcataa
     6121 aattacgtaa cattttatta catttagcgc atggttacaa attcttcaga tcctgtcggg
     6181 tgaatagcca ctgtgttgtc aaaatcagct ttcgttgcgc ccattttaat tgcaacagca
     6241 aaaccttgaa tcatttcatc aacaccaaaa ccaataccat gtaacccaac cactttttcc
     6301 ccttccccaa cacaaactaa tttcattcgg caaggttgac gatgttgtgt aaccgcagta
     6361 tacattgcag taaatgaaga tttatacact ttcacatttt ctgccccata ttgctcaatc
     6421 gcttttggct cacttaaacc aacagtacct attggtgggt ggctaaaaac gactgtcggt
     6481 actaagctat aatccaaatg ttcatttggt ttattattga acaaccgttc cgatagcctg
     6541 cgaccagctg caactgcaac aggagttaat tcaataccac cttcaataat atcaccaaca
     6601 gcatagatat ttggcacatt agtattttgg aatttatcca ctttgataaa accacgctcg
     6661 ttggtttcaa caccagcagc ctctaaatta atcttatctg tcgctggctc acgaccaata
     6721 gcccacacca agctttcaac agaggtttca cgaccatctt gcaattttaa tgtcaatgaa
     6781 ccatctgcat ttttaactac ttcttgagga gtcgcttgtg tatgtaattc gatcccatct
     6841 tgagctaata cttcaagcaa tgtatccact attaatgggt cttgattacg cattggagca
     6901 tgttgacgaa caaaaagatg ggtatttact cctaaactat taaatactcc agctaattca
     6961 actgcaatat accctgcccc cacaattgct acggacttcg gtaaagcatc tagtgcaaat
     7021 acaccatcag aagtgatacc gtattcagcc cctttcacat tgggtacagt tggacgacca
     7081 cctgttgcaa tcagaatatg gtctgctgtg acctcctcaa cggaaccatc agcataactc
     7141 acttctaatg ttttagcatc tttaaagcga gcaaaaccat ttaaaacatc aacattattt
     7201 tttgccagca cattaccata tgaggtatga atgcgcccaa tataggcctg gcgactctca
     7261 actaatttgg cataatcaaa attatttacc gtaatgtcaa aaccataatc aggggcgtaa
     7321 tggttaatcg cttctgcaac ttgtgcacca tagaacatca cttttttggg tacacatccc
     7381 acgttcacac aagtgccacc taaatgtttt gcttcaataa tggcacattt ttgcccatag
     7441 cttgctgctc gattaattga agcaatacca ccactaccac cgccgatagc aatataatca
     7501 taatgtttag ccataagcta ttcctcgatc tataaaaaca aaagcaactt attcgttaag
     7561 cgctttatca aattaaattt gcatagaaga ctaatgtttc atatgccctg aattcaacat
     7621 tttatcaata tacgccatcg ctaatgctga aacaataaag gctaagtgaa taactacttg
     7681 ccacatcatt tgcttttcat caagatttga agcattgata aatgtttgta a
//
LOCUS       DANLYI010000110         7694 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.110, whole genome shotgun sequence.
ACCESSION   DANLYI010000110 DANLYI010000000
VERSION     DANLYI010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 7694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7694
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.110"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            127..1110
                     /gene="epmA"
                     /locus_tag="PXD81_001658"
     CDS             127..1110
                     /gene="epmA"
                     /locus_tag="PXD81_001658"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005605446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HDL5537670.1"
                     /translation="MNNLQLDNIDWQPTASIPHLIQRSKIITEIRKFFTDRGLLEVET
                     PILSEFSVTDVHLSTFNTQFQSPFSPEAKTLHLMTSPEYHMKRLLAAGSGPIFQLCKV
                     FRNEEAGKRHNPEFTMLEWYRPHFDMYRLINEVDDLLQQILDCEPAESFSYQFVFQTY
                     VGLDPLSATKAQLVEKARKHGLQCDDDEQRDTLLQFLFSEVVEANIGQERPTAVYHFP
                     STQAALAQISSEDHRVAERFEFYYKGLELCNGFHELDDADEQIHRFEQDNLQREKMGL
                     EPQQLDVRFLAALKAGFPNCSGVALGVDRLLMLAMDAEKIEEVISFGIEKA"
     gene            1192..2082
                     /gene="rarD"
                     /locus_tag="PXD81_001659"
     CDS             1192..2082
                     /gene="rarD"
                     /locus_tag="PXD81_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HDL5537671.1"
                     /translation="MNISGKLQGWHYALLCYLTWGTFPIYWYPINQSGMPAEQILAQR
                     VLWSALFAVFLLLIFQQGRAVINAFKQPKVLGLFVLSSFMIGVNWLVYLWAIVNEHIL
                     DASLGYFINPIFNVFVGRLILKEQLNKAQLLALLFAISGILWLAIPAGQIPWVALILA
                     ASFCIYGLIRKLAPMEPLSGLALETLLMSPFAITYLCFCYHQNILVFSELNPLQMAVL
                     LCSGIATTLPLLWFAMGARRISMSLLGMLQYISPTLQFLCGVLIFNETLSAERAVGYI
                     LVWIGVGVFLLGMKKAGVKR"
     gene            complement(2093..2704)
                     /locus_tag="PXD81_001660"
     CDS             complement(2093..2704)
                     /locus_tag="PXD81_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL5537672.1"
                     /translation="MKYEAKPLIISPNHIQQRLHGKLLARALLIIALLMLAKFIYIAS
                     LSNYFTFYLMEKFQLNIAMAQLSLFTFLGAVALGAFVGGPIGDKIGRNAVIWFSFLGM
                     APFALLLPHADLFWTIVCAIFAGFIMSSAFSAMVVYAQEAVPGRVGLISGTMFGLMFG
                     IGGLSAAVLGFFADEYGLESIFKACAYLPLLGFAALGLPKRNI"
     gene            complement(2732..3267)
                     /locus_tag="PXD81_001661"
                     /pseudo
     CDS             complement(2732..3267)
                     /locus_tag="PXD81_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251153.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            3459..5468
                     /locus_tag="PXD81_001662"
     CDS             3459..5468
                     /locus_tag="PXD81_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HDL5537673.1"
                     /translation="MDLIKKLNTGSTFRVPIFLPSLLFVSFVAVFCIIFPQQAQTSLD
                     TIKNSLFQHFSWFYIFAGSIFFLFLIFLSFSRLGDIKLGADTDEPEFGFGSWIAMLFA
                     AGMGIGLMYFGVAEPILHYLKPVQQNLTEPERMKEAMMTTFYHWGIHAWAIYGVIALA
                     LAYFGFRYKLALTIRSGFYPLLKHRISGFWGHLIDIIALCSTIFGLTTTLGFGVMQVS
                     AGFNNLGLIEQSNFTVLAIIVTVAMALAVLSAVSGVGKGVKILSEINLTLAGLLLIFV
                     IITGPTLLLFSSFTENLGYYFSSLLEMSFRTFAYEPEHQGWLSGWTVLYWAWWASWAP
                     FVGLFIAKISKGRTIREFILGVLFVPSLFNILWMTSFGSSAIWFDQQTAGALAEVSGN
                     TEQLLFTFFEQLPFGSIASFVAVIVISIFFITSADSGIFVLNSIASQGEENAPKWQSV
                     LWGALLAILALSLLYSGGLASLQTMTLIIALPFTFIMLILCIGLWKGLMVDNQYFNKK
                     FSQGSQHWAGKDWKQRLEKIINPSNKQDVRHFFIKVARPAFLELIEEFESYGLIAKMN
                     FTNEQNPKLEFEVVKENLRNFIYGIESVPRELSDLVVGDDNLPNIEQNTIYEPITYFL
                     DGRKGYDVQYMTKEELIADVLQQYERFINLAMDNSHDLMTADFNH"
     gene            complement(5533..6000)
                     /locus_tag="PXD81_001663"
     CDS             complement(5533..6000)
                     /locus_tag="PXD81_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HDL5537674.1"
                     /translation="MKSLKLKIPPPAWFLLCAALMWLVAQYVPVSLPNYQHTAIFFSG
                     LAVAIIGGAVAVSALWVIHQAKTTYSPFRPQHTAKLVTWGIYRKSRNPMYLSLLLGLI
                     AWALWLGAMLAWLVLPLFVWLITYFQIKPEEQILAQKFGTHYAKYCNQVRRWL"
     gene            complement(6002..7576)
                     /gene="murJ"
                     /locus_tag="PXD81_001664"
     CDS             complement(6002..7576)
                     /gene="murJ"
                     /locus_tag="PXD81_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HDL5537675.1"
                     /translation="MSKKLLRSGMIVSFMTLISRILGLIRDIVVATILGTGVSADIFL
                     FANRIPNFLRRLFAEGAFSKAFVPVLAEYNADNDPNKTREFIAKVSGTLGGLVTVVTL
                     VAMIASPVVAALFGTGWFLDWLYDGPNAEKFTQASFLLKITFPYLWFITFVALSGAVL
                     NTIGKFGVMAFSPVLLNVAIIAVALWGRDYFDSPDIALAWGVFLGGLLQFLFQIPFMK
                     KEGLLVKPKWAWKDEGVTKVRKLMIPALFGVSVTQLNLLLNQVIASFLITGSISWMYY
                     ADRLIEFPLGLFGIAISTVVLPSLSRIAKNKELTEIQRGEHFQNTMDWGVRMVLLLGI
                     PAMIGMAVLAQPIIMTMFMRGKFGFEDVLATSYPLWVMCLGLNSYMLISVLANGFYAN
                     QNTKTPVKVGIIAALSNICFGLAFAPFLGYIGLALASACSALVNVSLLYVNLSKNGYY
                     KVSHKTVLFVLKLFIAACVMGALVAYFTPEIELWALMTMWQKVYWLIWLMILAGGSYF
                     ASILVLGIRKKDFRSA"
BASE COUNT         2281 a         1680 c         1544 g         2189 t
ORIGIN      
        1 taaaatgaga aaatttaatt tgttgcaaaa aagttaaaac cctctcttta tttttcttga
       61 tcggtaacta gggcaactca cttttttagg ctaaaatagc ctcacatttt caagcggtca
      121 ttttttatga acaatttaca actcgataat attgattggc aaccaacagc ctctattcct
      181 cacctgatcc aacgttcaaa aatcattacg gaaatccgca agttctttac cgatcgaggg
      241 cttttagagg tagaaacccc gattttaagc gaattttcgg tgacagacgt tcatctttcg
      301 actttcaata cgcagtttca atcgcctttt tcccctgagg cgaaaacact ccatctgatg
      361 accagcccgg aatatcatat gaagcgacta cttgctgctg ggagtggacc gattttccaa
      421 ctgtgtaaag tatttcgcaa cgaggaagcc ggtaagcgac acaaccctga atttacgatg
      481 ctggagtggt atcgcccgca ttttgatatg tatcgcttaa tcaatgaagt ggatgattta
      541 ctgcaacaaa ttttggactg cgaaccggca gaatcattca gctatcaatt tgtgttccaa
      601 acctatgtcg gattagatcc gctttctgcc accaaagcac aacttgtgga aaaagcccga
      661 aaacacggct tgcaatgtga tgatgatgaa cagcgtgaca ccttgttaca atttttattc
      721 agcgaggttg tcgaagccaa tatcgggcaa gaaagaccaa ctgccgttta tcatttccct
      781 tccacccaag cggcattagc tcaaattagc tcggaagatc accgtgttgc cgaacgtttt
      841 gagttttact acaaaggctt ggaactctgt aacggcttcc acgaattaga cgatgctgat
      901 gagcaaattc atcgcttcga gcaagataat ctgcaacgtg aaaaaatggg gctggagcca
      961 cagcaactag atgtgcgttt tcttgccgca ctcaaagcgg gttttccaaa ctgctcaggt
     1021 gtcgcccttg gggttgatag gctgttaatg ctggcaatgg atgcggaaaa aattgaagag
     1081 gttatttctt tcggcattga aaaagcctaa ttttttatgc cgttttgtga tttgcaattt
     1141 gcaaaaaaat cgacaaatat gaccgcttgt acactcgttt aggaaagcaa aatgaatatt
     1201 tcaggcaaat tacagggttg gcactatgcc ctactgtgct acttaacttg gggaaccttt
     1261 ccgatttatt ggtatccgat caaccaatcg ggaatgcctg ccgagcaaat tttggctcaa
     1321 cgggtgcttt ggtctgccct atttgctgtg tttctgctgc tgattttcca acaagggcga
     1381 gcggttatta atgcctttaa acagccaaag gtactcgggc tgttcgtgct ttcttctttt
     1441 atgattggcg tgaactggct ggtttactta tgggcaatcg tcaatgagca tattttagat
     1501 gccagtctag gttattttat caaccccatt ttcaacgtgt ttgtcggtag gcttattcta
     1561 aaagagcagc tgaataaagc ccaattattg gctctgctgt ttgcaattag cggcatttta
     1621 tggcttgcta ttcctgccgg acaaattccg tgggtcgcat tgattctagc cgccagtttc
     1681 tgtatttacg ggctgattcg taaacttgcc ccaatggaac ctttgtctgg tttggcgtta
     1741 gaaactttgc tgatgtcgcc ttttgccatc acctatctct gcttttgtta ccaccaaaac
     1801 attttggtat tcagtgaact caatccttta caaatggcag tgctactctg ctccggcatt
     1861 gccactacct taccattgct atggtttgca atgggtgcaa gacgcatttc aatgtcgcta
     1921 ctcggtatgc tgcaatatat ttcacctacc ctgcaatttt tatgcggtgt gctgattttt
     1981 aatgagacac tttccgccga acgtgctgta ggctacattc tggtttggat tggggtgggg
     2041 gttttcttat tgggtatgaa aaaagctggt gtcaaacgct aaaaataaaa cgttaaatat
     2101 ttcgttttgg tagccctaat gcagcaaaac caagtagtgg caaataggca caggctttaa
     2161 aaatactctc caagccatat tcatccgcaa aaaaacccaa tacggcggca cttaagccgc
     2221 caataccaaa cattaaaccg aacattgtgc cggaaatcag ccctactctg cctggtacgg
     2281 cttcttgagc ataaaccacc atagcggaga acgctgatga cataataaaa ccggcaaaaa
     2341 tcgcacacac aatcgtccaa aagagatctg cgtgaggcaa caacaaagca aaaggtgcca
     2401 tacctaagaa agagaaccaa atcaccgcat ttcgtccaat cttatcgcca atcggcccgc
     2461 cgacaaaagc gcctaaagca actgcaccta aaaaggtaaa tagagagagc tgtgccattg
     2521 ctatattgag ttggaatttt tccattaaat aaaaggtgaa atagttactg aggctcgcaa
     2581 tataaataaa tttcgctaac atcaataaag cgataattaa taatgctctg gctaataatt
     2641 taccgtgtaa gcgttgttga atatggtttg gtgaaataat gagcggtttg gcttcatatt
     2701 tcacccaacg gcttacttta tataaaaccc atcataatgc caataataca aatgcaacaa
     2761 cccaaatcac cgcactttgc cctaacggca caataaataa tgcaactaag agcggaccta
     2821 acgccgatcc actgtttcca cctacttgga aaagcgactg agctgtacca aatttaccgc
     2881 ccgatgccat tcgagccacc cgagaggctt cagggtgaaa aatggcagag cctatcccca
     2941 ttaacgcagc ggctatcagt aacacgccaa aattcggcga aaaggcaagt aaaatcacgg
     3001 aaagaagcgt aatcagcata ctgacgggca ataaattcgg ttttgggtat cggtataaag
     3061 cccaataccc ggctgaataa tagatgcggt aatttgataa atgagtgaaa tcataccaat
     3121 ttgagcaaaa ctcagttggt aattttcttt tagcattgga tagatcgaaa ccattactgc
     3181 ttgaactaaa tcattcaaca aatgagaaag ggaaatcagc agtaaaatgg gcaaaaccat
     3241 tttttgagcg ttatgttgtg tgtgcattct ttattcgttt tttataaatg aaaaatattc
     3301 tactcgcaaa taatgaatgt gcaagcggtc aaatttcccg atttttttac caaacccatt
     3361 aggttgcaat attattacca atacgtctta aatttgttat ttttatatct ttttgctata
     3421 ttcaagcctc taaccattca aaaatagagg aaatagcatt ggatttaatc aaaaaattaa
     3481 acacaggaag tacctttagg gtaccgattt tcctaccgag tttactcttt gtcagctttg
     3541 ttgccgtttt ctgtatcatc tttccacagc aagcacaaac ctcacttgat accatcaaaa
     3601 atagtctctt ccaacatttt agctggttct atatttttgc aggctctatc tttttcctgt
     3661 ttctaatttt tctctctttc agccgattgg gtgatattaa attaggggca gataccgatg
     3721 agcctgaatt tggttttggc tcttggattg cgatgttatt cgccgccgga atggggatcg
     3781 ggttaatgta ttttggggta gcagaaccta ttttgcatta ccttaaaccc gtccaacaaa
     3841 atttaactga gccggagcgt atgaaagaag cgatgatgac aacgttctat cattggggta
     3901 ttcacgcttg ggcaatttat ggtgtgattg ccttagctct tgcttatttt ggcttcagat
     3961 ataagttagc actcactatt cgttccggat tttatccctt actaaaacat cgtatttcag
     4021 gcttctgggg gcatttaatt gatattattg ccctttgtag cacgattttc ggtttaacga
     4081 ctacacttgg ctttggggtg atgcaggtca gtgctggctt taacaatcta ggtttaattg
     4141 aacagagcaa ttttactgtt cttgcgatta tcgtaacagt agcaatggct cttgccgtgt
     4201 tatctgccgt ttcgggcgta ggcaaagggg ttaaaatctt aagtgaaatc aatctcacat
     4261 tagccggatt gctacttatt tttgtgataa tcaccggccc aactctatta cttttctcaa
     4321 gcttcaccga aaatttaggc tattatttta gctcgctgct tgagatgagt ttccgtacct
     4381 tcgcttatga accggaacat caaggctggc taagcggctg gacggtcctt tattgggcat
     4441 ggtgggcatc ttgggcgcca tttgttggtt tgtttattgc caagatctct aaaggcagaa
     4501 ccattcgtga atttatttta ggggtgctat ttgttccatc gctgtttaac attttatgga
     4561 tgaccagctt cggcagctct gccatttggt tcgatcaaca aactgccggt gctttagctg
     4621 aagtcagcgg caataccgaa caactgttat ttaccttttt tgagcaatta ccgtttggct
     4681 ctattgcctc tttcgttgcc gtcattgtta tcagtatttt ctttatcacc tctgccgact
     4741 cggggatttt tgttctcaac agcattgctt cacaaggcga agaaaatgca ccgaaatggc
     4801 aaagcgtgct ttggggagca ttattagcca tcttagcgtt atcactactc tattcgggtg
     4861 gcttggcttc tctgcaaaca atgacactga ttatcgcctt accatttacc ttcattatgc
     4921 tgattctctg tatcggctta tggaaaggat taatggtaga taaccaatac ttcaacaaaa
     4981 aattctcgca aggtagccaa cattgggcgg gtaaagattg gaaacaacgc ttggagaaaa
     5041 tcatcaaccc aagcaataag caagatgtcc gtcacttctt tattaaagtt gccagaccag
     5101 catttttaga acttatcgag gaatttgaaa gctatggctt aatcgctaaa atgaatttca
     5161 ccaacgaaca aaacccgaaa ttagagtttg aagtggtgaa agaaaattta cgcaatttca
     5221 tttacggcat tgaaagtgtg ccacgggaat tatcggattt ggtggtaggt gacgacaacc
     5281 taccgaacat tgagcaaaat accatttacg agccgattac ttatttctta gacgggcgga
     5341 aaggttatga tgtgcaatat atgaccaaag aagagttgat tgccgacgtg ctgcaacagt
     5401 atgaacgctt tatcaattta gcgatggaca actcgcacga cttaatgacg gctgatttca
     5461 atcactaacc ccacaagcgg tgagattttc ggaaaaattt gcaaatttcc aacaaaatct
     5521 caccgcttgc atttaaagcc aacgccttac ttggttgcaa tatttggcat aatgtgtgcc
     5581 gaatttttgg gctaaaattt gctcttccgg cttgatttgg aaataggtta tcaaccatac
     5641 aaacagcggt aaaaccagcc acgccaacat tgcccccaac cataatgccc acgcaatcaa
     5701 acctaatagt aacgaaagat acattgggtt tcggcttttg cggtaaatgc cccaagtgac
     5761 caatttagcg gtatgttgtg gtcgaaaagg actgtatgtc gttttcgctt ggtgtatgac
     5821 ccaaagtgcc gagacggcaa ccgctccgcc aattatcgcc acagctaaac cgctaaaaaa
     5881 tatggctgta tgctgataat tcggcaagct caccggcaca tattgagcta ccagccacat
     5941 taaagcggca caaagcaaga accaagccgg cggcgggatt ttcagtttta atgatttcat
     6001 tttatgccga gcggaaatct tttttacgaa tgcctaatac caaaatagag gcaaaataac
     6061 taccgccggc taagatcatc agccaaatca gccaatacac tttctgccac attgtcatta
     6121 atgcccaaag ttcaatctca ggcgtgaaat aggcaaccaa cgcccccatt acacaagcgg
     6181 cgatgaatag tttcaacaca aaaagcacgg ttttatggct tactttatag taaccatttt
     6241 ttgagagatt cacatacagc aagctcacat tcactaatgc cgaacacgca gaagctaacg
     6301 ctaaaccaat gtatcccaaa aacggagcaa acgccaagcc aaagcaaata ttacttaagg
     6361 cggcaataat cccaactttt accggtgttt tggtgttttg gttggcataa aaaccgtttg
     6421 ccagcacgct aatcaacata tagctgttta accccaagca cattacccaa agtggataag
     6481 aggttgccag cacatcttca aaaccaaatt taccccgcat aaacattgtc ataataatcg
     6541 gctgagccag cactgccata ccgatcattg ccggaatgcc gagcaacagc accattctca
     6601 ccccccaatc cattgtgttt tgaaagtgtt ctccacgttg gatttcagtg agttctttgt
     6661 ttttcgcaat acgggaaagg cttggcagca cgacggtgga aatcgcaatt ccaaataaac
     6721 cgagcggaaa ttcaattaag cggtcggcat aatacatcca actaatcgag ccggtaatca
     6781 aaaacgaggc aatcacttgg tttagcagta aatttagctg ggtaacggaa acgccaaata
     6841 aagccggaat cattaattta cggactttag tgacaccttc gtctttccac gcccattttg
     6901 gcttaaccag taagccctct tttttcataa atggaatttg gaataaaaat tgcaataagc
     6961 cgcctaaaaa taccccccac gctaaggcaa tatccgggga atcgaaataa tctctgcccc
     7021 ataacgctac cgcgataatc gccacattca gcaacaccgg ggaaaatgcc atcaccccaa
     7081 acttaccgat ggtatttaac accgcaccgg ataacgccac aaaggtaata aaccacaaat
     7141 aagggaaagt gattttgagc aaaaaagagg cttgggtgaa tttttcggca tttggcccgt
     7201 cgtaaagcca atctaaaaac caacctgtac caaaaagagc ggcaaccaca ggcgaggcaa
     7261 tcattgccac aagggtaaca acagttacca aacctcctaa tgtacccgat actttggcaa
     7321 taaattctct cgttttgttt ggatcgttat ccgcattata ttccgccaac accggcacaa
     7381 aggctttaga aaatgcccct tcggcaaaca aacggcgaag aaaattggga atacggttgg
     7441 caaacaggaa aatatcggca ctcacgcctg tgcctaaaat ggttgccacc acaatgtctc
     7501 gaatcaaacc gagtatgcga gaaattaagg tcataaagct tacgatcatt cctgatctta
     7561 aaagtttttt gctcataata aagagtaata ttagtaaaat tattgaaaaa tcagaccgct
     7621 tgtagtctga tcctaaaaat cgatcataat tttagcagtt tgttatagga aaatcagaaa
     7681 aaaactgtta aaat
//
LOCUS       DANLYI010000111         7618 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.111, whole genome shotgun sequence.
ACCESSION   DANLYI010000111 DANLYI010000000
VERSION     DANLYI010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 7618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7618
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.111"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            170..670
                     /locus_tag="PXD81_001665"
     CDS             170..670
                     /locus_tag="PXD81_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HDL5537676.1"
                     /translation="MQTINEIKEEIYPIFEPIIGTIRLKTYFSYYAVFKDGLMIALYQ
                     NGATYLRIAKKDIEYIRQYPKTCILCDEKIGIQSRKFHYIPQVLIDDTPKFSSLLFGI
                     IEELRTEKYKISQKRATQIRNLPNMNLKLERMLKKIGIHSIHDFTQEGYMSTFIKLIL
                     HRRLTV"
     gene            958..2352
                     /gene="fumC"
                     /locus_tag="PXD81_001666"
     CDS             958..2352
                     /gene="fumC"
                     /locus_tag="PXD81_001666"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005567406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HDL5537677.1"
                     /translation="MEFRIEKDTMGEVQVPADKYWAAQTERSRNNFKIGPEASMPAEI
                     IEAFGYLKKAAAYANHDLGVLAVEKRDLIAKACDEILANKLDDQFPLVIWQTGSGTQS
                     NMNVNEVVANRAHVLNGGKLGEKSIIHPNDDVNKSQSSNDTYPTAMHIAAYKKVVEHT
                     LPCVKRLQKTLAEKAEAFKDVVKIGRTHLMDATPLTLGQEFSAYAAQLAFGIKALENT
                     LPHLRELALGGTAVGTGLNTPQGYDVKVAEYIAKFTGLPFVTAQNKFEALATHDAIVE
                     THGALKQLAVSLYKIANDVRLLASGPRSGIGEILIPENEPGSSIMPGKVNPTQCEAMT
                     MVCAQVLGNDVTISFAGTQGHFQLNVYKPVMAYNFLQSAQLLGDACVSFDEHCAVGIE
                     PNYPRIKQQLDQSLMLVTALNTHIGYENAAKIAKTAHKNGTTLKEEAINLGLVTAEQF
                     DEWVRPEDMVGSLK"
     gene            2479..2589
                     /locus_tag="PXD81_001667"
     CDS             2479..2589
                     /locus_tag="PXD81_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005696979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDL5537678.1"
                     /translation="MKKLILIALVTTLGLSACGVKGPLYFPEQAPKHEQK"
     gene            2694..3944
                     /gene="lysA"
                     /locus_tag="PXD81_001668"
     CDS             2694..3944
                     /gene="lysA"
                     /locus_tag="PXD81_001668"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005620569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HDL5537679.1"
                     /translation="MNHFEYKNNQLFAEEVAIADIIEQYGTPAYVYSRSTLERHWHAF
                     NNAFGSHPHLVCFAVKSNSNIALLNVMARLGSGFDIVSQGELERVLAAGGEASKVVFS
                     GVAKSHSEIKRALEVGIRCFNIESVSELHRINQVAGELGKIAPISLRVNPDVDAHTHP
                     YISTGLKENKFGVSVTQAREVYRLAKTLPNVKITGMDCHIGSQLTELQPFLDATDRLI
                     ILMEQLREDGIELHHLDLGGGLGVPYNGEEPPHPTEYAKALLEKLKGYENLEIIIEPG
                     RAITANAGILVTKVEYLKQNEDRNFAIVDTGMNDMIRPALYEAYMQITEVDKTLNRDK
                     AVYDVVGPICETSDFLGKQRELAISEGDLIAIRSAGAYGAAMSSTYNSRPQAAEIMVD
                     GNQAHLIKARATFADLWRLEKLLP"
     gene            3958..4587
                     /locus_tag="PXD81_001669"
     CDS             3958..4587
                     /locus_tag="PXD81_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009875238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HDL5537680.1"
                     /translation="MKLYGLTGACSFVPHVALEWVKLRANQDYAFQAVSREFIKSAEY
                     LALNPRGNVPLLVDGDLALTQNQAIVHYLDELYPEAKLFGSKTARDKAKAARWLAFFN
                     SDVHKSFVPLFRLPSYAEGNETLTKTIRQQSAEQILEQLAFANAHLENHIFFGEEISV
                     ADAYLYIMLNWCRLLGLDFSHLSQLSAFMQRVEADQGVDNVREQEGLKG"
     gene            4658..6103
                     /gene="tldD"
                     /locus_tag="PXD81_001670"
     CDS             4658..6103
                     /gene="tldD"
                     /locus_tag="PXD81_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005618007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HDL5537681.1"
                     /translation="MLNQVSNSLLAPSGLELSHLTQVLDHFSGRHIDYADLYFQLSQD
                     ESWSLEDSIIKEGGFYIDRGFGVRAISGEKTGFAYADQITLKQLEQCAAAARSISPTS
                     GQLSVKNFKKTTACSRYPAINPLETISREQKVELLHLVDKTARMADPRVIQVNANLSA
                     VYEEMLVAATDGTLAADIRPLVRLSVSVLVEQNGKRERGSAGAGGRFGLDWFLAPSET
                     GFSRAESLTKEAVRQALVNLTAIPAPAGTMPIVLGAGWPGILLHEAVGHGLEGDFNRK
                     ESSLFSGKIGQMVTSELCTIVDDGTVPNMRGSITVDDEGVPSQRNVLIENGILKGYMQ
                     DKLNARLMGVAPTGNGRRESYAHLPMPRMTNTYLTEGNHEFEEMIESVEYGLYAPHFS
                     GGQVDITSGKFVFSTAEAYLIEKGKITKPVTGATLIGSGIEAMQQVSMVGKKMELDPG
                     IGTCGKEGQSVPVGVGQPTVKLDKITVGGRG"
     gene            6167..7048
                     /gene="prmA"
                     /locus_tag="PXD81_001671"
     CDS             6167..7048
                     /gene="prmA"
                     /locus_tag="PXD81_001671"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HDL5537682.1"
                     /translation="MAWVQIRLNSTDKQAEEISDFLTEIGAVSVTFMDSQDTPIFEPL
                     PGETRLWGNTDVVGLFDAETDMNLIVEALKTSKLVDENFAHKIEQIEDKDWEREWMDN
                     FHPMQFGKRLWICPSWREVPDPNAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLEGK
                     TVIDFGCGSGILAIAALKLGAKQAIGIDIDPQAILASQNNAEANGVAEKLQLFLAKDQ
                     PRELQADVVVANILAGPLKELAPNIITLVKPNGNLGLSGILATQAESVCEAYSEAFTL
                     DPVVEKEEWCRITGRKI"
     gene            complement(7223..7298)
                     /locus_tag="PXD81_001672"
     tRNA            complement(7223..7298)
                     /locus_tag="PXD81_001672"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7263..7265),aa:Trp,seq:cca)
     gene            complement(7364..7440)
                     /locus_tag="PXD81_001673"
     tRNA            complement(7364..7440)
                     /locus_tag="PXD81_001673"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7404..7406),aa:Asp,seq:gtc)
     gene            complement(7451..7566)
                     /gene="rrf"
                     /locus_tag="PXD81_001674"
     rRNA            complement(7451..7566)
                     /gene="rrf"
                     /locus_tag="PXD81_001674"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         2280 a         1687 c         1662 g         1989 t
ORIGIN      
        1 caatatttcc aatacttcat ttgaagtggc tcaaataaat tgtatttatg cattttttta
       61 ttcaaacttt tcgagccact tcacaaatct aaaaaatcct agttgaaatt taatccataa
      121 gagtatttat tattaaataa gtttcatgac cttacatcgg agtacatata tgcaaacaat
      181 caatgaaatt aaagaagaaa tctaccctat atttgagcct atcattggaa caattcggct
      241 aaaaacctac ttttcctact acgcagtttt taaggatgga ttgatgattg cattgtatca
      301 aaatggagca acttatttac gtattgcgaa aaaagatatt gaatacattc gtcagtatcc
      361 taaaacctgt atcttatgcg atgaaaaaat tggtatacaa agtagaaaat ttcattatat
      421 tccgcaagtt ctcatagatg atacaccaaa atttagctcg ctactctttg gaattataga
      481 ggaactccga acggagaagt acaaaataag ccaaaagcga gctacacaaa tccgaaacct
      541 accaaatatg aatttaaagt tagaaagaat gttgaaaaaa atagggattc attccattca
      601 tgattttact caagaagggt atatgtcaac ttttatcaaa ctgattctcc accgaagact
      661 tactgtttaa gctaaacgga gctttaaatc accaatatat ttacaccttc acagatcaac
      721 aaaaaaggga actaatgcaa gaagcaaatc aagcgctata taattcaggc ctaagaaaaa
      781 gatttaacgc cctgtagtta aataatgtga tccaaatcat aaaatataaa aaaaccaata
      841 aggaattgca attcgcagtt cctttctttt tcttttatat gctagtatca ccttatacct
      901 ctaaattatt agtgcctact gattttcaat taatccaaca atttaggaga gcgcatcatg
      961 gaattccgta tcgaaaaaga cacaatgggc gaagtgcaag ttcctgccga taaatactgg
     1021 gcggcgcaaa ccgagcgttc acgcaataac ttcaaaatcg ggccggaggc atcaatgccg
     1081 gcggaaatta tcgaggcgtt tggctatctg aaaaaagcgg ctgcctatgc taatcacgat
     1141 ttaggcgtac tagcggtcga aaaacgggat ttaattgcaa aagcctgtga tgaaattttg
     1201 gcgaacaaac tcgatgatca attcccgctg gtgatttggc aaaccggctc gggtacgcag
     1261 tcaaatatga acgtgaatga agtcgtggca aaccgtgcac acgtgttaaa cggcggcaag
     1321 ctgggtgaaa aatcgattat tcacccgaat gatgatgtga ataaatccca atcttctaac
     1381 gacacctacc caactgcaat gcacatcgct gcttacaaaa aagtggtgga acacacccta
     1441 ccttgtgtga aacgtctgca aaaaacctta gcggaaaaag ccgaagcctt taaagatgtg
     1501 gtcaaaatag ggcgtactca cttaatggat gctaccccgc tcacgctcgg tcaagagttc
     1561 tctgcctatg cagcacagtt agcattcggc atcaaagcat tggaaaacac cctgccacac
     1621 ctgcgtgagc tggcactcgg cggcacagca gtcggcaccg gcttgaatac cccgcaaggt
     1681 tacgatgtga aagtggcgga atacattgcg aaattcaccg gtttaccgtt cgttaccgcc
     1741 caaaacaaat ttgaggcatt agcgactcac gatgcgattg tcgaaaccca cggtgcatta
     1801 aaacagcttg cggtgtcgct ctacaaaatt gccaacgatg tccgtttatt agcctccggt
     1861 cctcgttcag gcattggcga aattctgatt ccggaaaacg agccgggttc atcaattatg
     1921 ccgggtaaag taaacccaac ccaatgcgaa gcaatgacaa tggtatgtgc ccaagtatta
     1981 ggcaacgatg taaccatctc ttttgccggc acgcaagggc atttccaact gaatgtctat
     2041 aaaccggtga tggcgtacaa cttcctgcaa tcggctcaac tgctaggcga tgcgtgcgtg
     2101 tcgtttgacg aacactgtgc agtcggcatt gaaccgaact accctcgcat taaacaacag
     2161 ctcgaccaat cgctgatgtt agtgaccgca ttaaatactc acattggtta tgaaaatgca
     2221 gcgaaaatcg ccaaaactgc ccacaaaaac ggcacaacac tgaaagaaga ggcgattaac
     2281 ttaggcttag tcacggcaga acagttcgat gaatgggttc gtccggaaga tatggttggc
     2341 agcttgaaat aagcccaacc cttctcttgt aatctgattt acacctcaat ctaccggtaa
     2401 gattgaggtg taatttttta tagcgagggt actgattttg ttttagaatg aagcatcatt
     2461 ttttaagcgg aaaaacctat gaaaaaactc attttaatcg cattagttac tactttaggc
     2521 ttaagtgctt gtggggtcaa aggcccactc tacttccctg agcaagcccc taaacatgaa
     2581 caaaaataag cattgcaagc ggtctgtttt ggctaaaatt ttgcaaattt ttgtgaaaaa
     2641 atgaccgctt gttgcctctt ttctttcaaa atcccaaaca caacggactt tcaatgaacc
     2701 attttgaata taaaaacaac caactttttg ctgaagaagt cgctattgcc gatattatcg
     2761 agcaatacgg cacgccagct tatgtttact ccagatctac ccttgaacgc cactggcacg
     2821 cattcaataa tgcgtttggc tcgcacccac atttagtttg ctttgcagtc aaatccaact
     2881 ctaacattgc cttgttaaat gtaatggctc gtctcggttc aggctttgat attgtctctc
     2941 aaggcgagtt ggagcgtgtc ttagctgccg gcggcgaagc ctctaaagtt gtcttttccg
     3001 gtgtggcaaa atcgcacagc gaaatcaaac gagcattgga agtcgggatt cgttgcttta
     3061 acattgaatc ggtttccgaa cttcaccgca ttaaccaagt tgccggtgaa ctaggcaaaa
     3121 tcgcaccgat ttcactgcgg gtaaacccgg atgtggacgc ccacactcac ccatacattt
     3181 ccaccggttt aaaagaaaac aaattcggcg tgagcgttac tcaagcccgt gaagtgtatc
     3241 gcttagccaa aaccttaccg aatgtgaaaa ttaccggtat ggattgtcac atcggttcgc
     3301 aattaaccga attgcaacct tttttagatg caacggatcg cttaatcatc ttaatggagc
     3361 aactgcgtga agacggcatt gagctacacc acttagactt aggtggcggt ttaggcgtgc
     3421 cttacaacgg cgaagagcct ccgcatccga ccgaatatgc caaagccttg ctggaaaagc
     3481 tcaaaggcta tgaaaactta gaaatcatta ttgaaccggg gcgtgccatt accgcaaatg
     3541 ccggtatttt agtgacgaag gtggaatact tgaaacaaaa tgaagatcgt aattttgcga
     3601 ttgtcgatac cggtatgaac gatatgattc gcccggctct gtatgaagcc tatatgcaaa
     3661 ttacggaagt ggataaaacc ttaaaccgtg acaaagctgt ttacgatgtg gtcggtccga
     3721 tttgcgaaac ctcggatttc ttaggcaaac aaagagaatt ggcaatttct gaaggggatt
     3781 taatcgctat tcgttctgcc ggtgcttatg gggcggcgat gtcttcaacc tacaactctc
     3841 gcccacaagc ggcagaaatt atggttgatg gcaaccaagc ccatttaatt aaagcccgag
     3901 ccacttttgc tgatttatgg cgtttagaaa aattattacc ttaatgagga agaaaagatg
     3961 aaattatatg gattaaccgg tgcttgttca tttgtaccac acgttgccct agagtgggtg
     4021 aaattgcgtg ccaatcaaga ttatgcgttt caagctgtta gccgtgaatt tattaaatcg
     4081 gcggaatatc tggctttaaa ccctcgtggt aacgtaccat tgttggttga tggcgacttg
     4141 gccctcacgc aaaaccaagc tatcgtccac tatttagacg agctttatcc ggaagctaaa
     4201 ttattcggca gcaaaaccgc tcgtgataaa gccaaagcgg ctcgttggtt agcttttttc
     4261 aattcagatg tgcataaatc attcgtgcca ctgttccgct tgccaagtta tgcggaaggc
     4321 aatgaaacgc tcaccaaaac tattcgccaa caatcggctg agcaaatttt agaacaactg
     4381 gcttttgcga atgctcattt agaaaatcat attttctttg gcgaagaaat ttcggttgcc
     4441 gatgcgtatc tctacattat gctcaactgg tgtcgcctac tagggttgga tttctctcat
     4501 ctttcccaac tttctgcctt tatgcaacga gtggaggcag accaaggcgt agataatgtg
     4561 cgtgagcaag aaggcttaaa aggttaattc accattgttc atttaagacg gtagatttta
     4621 tgttaaaatc taccgtcttt tatttttagg aatcaccatg ctaaatcagg tttcaaacag
     4681 cttgcttgct ccaagtgggc ttgaattatc gcacttaact caagtgttag atcacttctc
     4741 cggccgtcat attgattatg ccgatctcta cttccaactc agccaagacg aaagctggag
     4801 cttggaagac agcatcatca aagaaggcgg tttttatatc gaccgagggt tcggtgtgcg
     4861 agccatatca ggcgaaaaaa ccggctttgc ctacgccgat caaatcacgt taaaacagct
     4921 tgagcaatgt gccgctgccg cacgttcaat cagcccaaca agcggtcaat tatcggtaaa
     4981 aaatttcaaa aaaacgaccg cttgttctcg ctacccggca atcaacccgc tagagaccat
     5041 cagccgtgaa caaaaggtcg aattgctcca tttggtggat aaaaccgctc gaatggcaga
     5101 tccgagagtg attcaagtga atgcgaatct ctcagcggta tatgaagaaa tgttagtggc
     5161 agcgactgac ggcacactgg ctgcggatat tcgcccgtta gtgcgtttat ctgtgtcggt
     5221 actggtggaa caaaatggca agcgtgaacg aggttcagca ggtgccggtg gtcgctttgg
     5281 tttagattgg ttcttagcac caagcgaaac cggctttagc cgtgcggaat cgcttaccaa
     5341 agaagccgtt cgccaagccc tcgttaatct cacagccatt cctgcaccgg caggcacgat
     5401 gccgattgtt ctcggtgctg gctggccggg cattttgctc cacgaagcag tcggacacgg
     5461 cttggagggt gattttaacc gtaaggaatc ttcgctcttc agtggtaaaa tcgggcaaat
     5521 ggtcacctct gagctttgca ccattgttga tgacggtacg gtgccgaata tgcgaggctc
     5581 aatcacggta gatgacgaag gcgtgccttc gcaacgtaat gtgttaattg aaaacggcat
     5641 tctaaaaggc tatatgcaag acaaactcaa tgcccgcctg atgggagttg ccccaaccgg
     5701 caacggcaga cgggaatctt acgctcattt gccgatgcca cgaatgacca acacctacct
     5761 caccgaaggc aatcacgaat ttgaagagat gattgaatcg gtcgaatacg gtttatatgc
     5821 tcctcacttc agtggtggac aagtggatat tacctccggc aaatttgtgt tctccaccgc
     5881 cgaagcctac ttaatcgaaa aaggcaaaat caccaaacct gttaccggtg caaccctgat
     5941 cggcagcggc atagaagcaa tgcaacaagt ttcaatggtc ggcaaaaaaa tggaactcga
     6001 ccccggcatc ggcacttgtg gcaaagaagg gcaatccgta ccagtcggcg tagggcagcc
     6061 tacagtgaag ttggataaaa ttacggtggg gggaagaggg taatctctcc ccgtttgcaa
     6121 aatataaaga aaatatcacc gcttgtagcg ataaaaggaa aacaaaatgg cttgggtaca
     6181 aattcgttta aacagtacag ataaacaggc tgaggaaatc agcgattttt taaccgagat
     6241 tggagcggtg tcagtgactt ttatggatag tcaagatacc ccaatttttg aacctcttcc
     6301 gggggaaacc cgtctttggg gaaataccga tgtggttggg cttttcgatg cggaaactga
     6361 tatgaatctt atcgtagaag cactaaaaac cagcaaattg gtggacgaaa actttgctca
     6421 caaaatcgag caaatcgaag acaaagactg ggaacgggag tggatggata atttccaccc
     6481 aatgcagttt ggcaaacggt tatggatctg cccaagctgg cgtgaagtac cagacccgaa
     6541 tgcagtgaat gttatgttag accctggttt agcatttggc accggcacac acccaacgac
     6601 ctcgctttgc ttacaatggt tagatagctt agatttagaa ggcaaaacgg ttatcgactt
     6661 cggctgtggc tccggtattt tagctatcgc tgcacttaaa ctcggtgcaa aacaagccat
     6721 cgggattgat atcgacccac aggcgatttt agctagtcaa aacaatgctg aggcgaacgg
     6781 cgtagcagaa aaactacaac tattcttagc caaagatcag ccaagagagt tacaagccga
     6841 tgtcgttgtt gcgaatattc tagccggccc actcaaagag ttagcaccga atatcatcac
     6901 gctggttaaa ccaaacggca atttaggctt atccggcatt cttgctactc aggctgaaag
     6961 tgtgtgtgag gcttatagcg aagcctttac gctagaccct gttgtagaaa aagaagaatg
     7021 gtgtcgtatt acaggccgca aaatctaata taagaagccc tgttttaaac agggcttact
     7081 ttttgataaa ttctactact ctttacctgt cttgggaatc gttctcccta aattaattaa
     7141 cacaagcggt agaaataaca ggaaaattta caaattacat aaatgaaaaa agccgactga
     7201 aatcagtcgg ctttctaaat aatggcaggg gcggagaggc tcgaactccc aacacccggt
     7261 tttggagacc ggtgctctac caattgaact acgcccctat tgataggtat tcattttgca
     7321 cttatagggg cgaattacta ttcgcccctg cattaaagat tattggcgga acggacggga
     7381 ctcgaacccg cgaccccctg cgtgacaggc aggtattcta accagctgaa ctaccgctcc
     7441 taaattaata gattgataat gccctactct cacatggcga atcaccacac taccatcagc
     7501 gttactgcgt tttacttctg agttcggtat ggagtcaggt agagccacag cactctggtt
     7561 atcaatcaaa ttctttcatt tagtcagttt actaaaatat agtttatact ttctaata
//
LOCUS       DANLYI010000112         7579 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.112, whole genome shotgun sequence.
ACCESSION   DANLYI010000112 DANLYI010000000
VERSION     DANLYI010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 7579)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7579
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.112"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            <1..878
                     /gene="rbsB"
                     /locus_tag="PXD81_001675"
     CDS             <1..878
                     /gene="rbsB"
                     /locus_tag="PXD81_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006995182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
                     /protein_id="HDL5537683.1"
                     /translation="KKLTTIASAVMLSLSVAATASAKETIALAVSTLDNPFFVSLKDG
                     AQKKADELGYKLVVLDSQNDPAKELSNVEDLTVRGAKVLLINPTDSEAVGNAVAIANR
                     NKIPVITLDRGAAKGEVVSHIASDNVAGGKMAGDFIAQKLGSGAKVIQLEGIAGTSAA
                     RERGEGFKQAIEAHKFNVLASQPADFDRTKGLNVTENLLSSKGDVQAIFAQNDEMALG
                     ALRAVSSAKKEVVIVGFDGTDDGVKAVESGKLAATIAQQPELIGSLGVETADKVLKGE
                     KVEAKIPVDLKVISK"
     gene            999..1928
                     /gene="rbsK"
                     /locus_tag="PXD81_001676"
     CDS             999..1928
                     /gene="rbsK"
                     /locus_tag="PXD81_001676"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HDL5537684.1"
                     /translation="MKKLTVLGSINADHVISVPYFAKPGETLTGHSCHIAYGGKGANQ
                     AVAAARLGAKVSFIGCIGSDDIGKAMKSAFEQDGIDTRSIKTIPNEMTGIAMIQVAES
                     GENSIVISAGANGHLDETVVAKCAAEITQADYLLMQLETPLPAIVQAAKLAQQNGTKV
                     ILNPAPARALPDELLNLLDMITPNETEAEILTGVKVVDEASAIQAAQVFHQKGIEKVL
                     ITLGSKGVFVSENGVGKIVAGFRVQAVDTTAAGDTFNGALVTAMLEDKPLDQAIRFAH
                     AAAAISVTRKGAQPSIPTRQETLDFLSQQAVSF"
     gene            1942..2952
                     /locus_tag="PXD81_001677"
     CDS             1942..2952
                     /locus_tag="PXD81_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HDL5537685.1"
                     /translation="MATMKDIARLAQVSTSTVSHVINNSRFVSEEIRERVQRVVKELN
                     YTPSALARSLKVNETKTIGMLVTATGNPFFAEVMAGVEQYCQQHQYNLIIATTNGDTE
                     RLQHHLQMLMQRKVDGLLLMCGDTRLNADESLNIPLPLVVLDWWFTELNADKIFENSI
                     SGGYLATKTLIEAGHSKIAIITGNLKKSLAKNRLEGYKKALAEANIPLNPNWVFESHF
                     DFAGGVNGMNALLAQSEKPTAVFACSDTIALGAYQAIWQQGLRIPQDISIIGYDDIEL
                     AKYLSPPLSTISQPKAELGRLAVETLLKRIRFKSTDFQTIMLEPELVLRHSIQTINKN
                     AE"
     gene            complement(2988..3617)
                     /locus_tag="PXD81_001678"
     CDS             complement(2988..3617)
                     /locus_tag="PXD81_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005622307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HDL5537686.1"
                     /translation="MTAPTQILLIDDHPLMRQGMRQLLELDHQFSVVGEASNGTDGIK
                     LALRLEPDVIILDLNMKGLSGLDTLRSLRAQGIDARIIVLTVSDEQSDVFALMDAGVD
                     GYLLKDTDTPELLDNIRKAAAGEVVLSETVRQHLLNRQPENGPLSCLTDRELDVLQWL
                     ATGMSNKQIAAQLFISEETVKVHIRNLLRKLNVHSRVAATVLYLEQQKG"
     gene            3815..5974
                     /gene="rlmKL"
                     /locus_tag="PXD81_001679"
     CDS             3815..5974
                     /gene="rlmKL"
                     /locus_tag="PXD81_001679"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018650943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HDL5537687.1"
                     /translation="MTNQTTYFATAARGFEEILKTELEQICAAECKVAQGGVHFTTTQ
                     KGAYQALLHSRLASRILLPLISTKIFSDSDLYASIVSFNWADLFDPRDTFYIDFSGTN
                     REIRNTQFGAMRVKDGIVDYFERKGFARPTVDKDNPDVRIHVYLDRENVVVSLDLSGD
                     ALHIRGYREETGKAPLRETLAAAIILRSGWKVGTPLVDPMCGSGTLLIEAAQMAANIA
                     PQLHRKHWGFNAWKGHQQAMWKAVLDEAFRNVELGAVGENCNSSLQKMFFGFDLDHRV
                     LTKAKQNAKNAGVDHLIQWQQGDVAALKNPIPEKIGTVVCNPPYGERLGTTPALIALY
                     SVFGQRLKQQFAGWNASIFSGEPELLNCLRLRSARQFKAKNGPLDCVQKNYSISEHNK
                     RSDLDENLQFEQNAQVAPDFANRLTKNIKKIEKWAKQQGIDAYRLYDADLPEYNLAVD
                     RYGDHIVVQEYQAPKNIDEQKARQRLLDAVSATLYVTGVETNKLVLKVRQKQKGTNQY
                     EKLANKGEYFYVNEYGAKLWVNLTDYLDTGLFLDHRLTRKMVGEMAKGKTFLNLFAYT
                     GSATVHAALNGAKSTTTVDMSNTYLNWAEQNLELNNLSLRHHRLFQADCLQWLAECRE
                     RFELIFVDPPTFSNSKRMENSWDVQRDHIELMKQLKRILTADGTIVFSNNKRGFKMDF
                     DGLAALGLTAENISHKTLPLDFERNPQIHNCWIVKNV"
     gene            6151..6630
                     /gene="trmL"
                     /locus_tag="PXD81_001680"
     CDS             6151..6630
                     /gene="trmL"
                     /locus_tag="PXD81_001680"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015432117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HDL5537688.1"
                     /translation="MLDIVLYEPEIPQNSGNIIRLCANCGFRLHMIEPFGFGWDDKKL
                     RRSGLDYHEFVDIQKYKNFEDFLERAKPKRLFALTTRGEQNHSDVKYELGDFLMFGPE
                     SRGIPKPLLAEMPMSQKIRIPMCKDSRSMNLSNSVAVVVYEAWRQFGYQNAVAAQSI"
     gene            complement(6724..7542)
                     /locus_tag="PXD81_001681"
     CDS             complement(6724..7542)
                     /locus_tag="PXD81_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bro-N domain-containing protein"
                     /protein_id="HDL5537689.1"
                     /translation="MQEIKLFEQKSVRSHYDEEKDKWYFSIIDVIAILTESLDPSAYW
                     RKLKQRLKAEGNETVTNCHGLKMPSADGKMRLTDVADTEQLFRLIQSIPSPKAEPFKQ
                     WLAKVGYERLQEINDPSQSLDRARENWQKMGRSEKWIQQRMMGQETRNKLTDYWKSSG
                     VEQPQDFAFLTNVIHQEWSGLSVQKHKSLKGLKSQNLRDHMSEAELLFTALAELSSRQ
                     IAESMQAVGVDENAQAGKQGGNIAKNARQELENKTGKSVVTGENFLPPAKNEEE"
BASE COUNT         2192 a         1540 c         1740 g         2107 t
ORIGIN      
        1 tgaaaaaatt aactacgatt gcatcagcgg ttatgttaag tttatcggtt gcagccactg
       61 cttcggcaaa agagactatc gctcttgcag tatcgacttt agataaccca ttctttgtga
      121 gccttaaaga cggtgcacag aaaaaagcag atgaattagg ctacaaatta gtggtgcttg
      181 attcccaaaa tgacccggct aaagagcttt ccaatgtgga agacttaacg gtgcgtgggg
      241 caaaagtgtt attgattaac ccaaccgatt cggaagcggt cggaaatgcg gtggctattg
      301 caaatcgcaa caaaattccg gttattacac ttgaccgtgg tgcagcaaaa ggcgaagtgg
      361 ttagccatat cgcttccgat aacgttgccg gcggcaaaat ggcgggagat ttcattgctc
      421 agaaattagg ctcaggggcg aaagtgattc aattagaagg cattgccggc acttccgccg
      481 ctcgtgaacg tggtgaaggc tttaaacaag cgattgaagc acataaattc aatgtattag
      541 ccagtcaacc ggctgatttt gaccgcacta aaggcttaaa tgtgactgaa aacttgctct
      601 caagcaaagg ggacgtacaa gctatttttg ctcaaaatga tgaaatggca ctaggagctt
      661 tgcgtgcagt gagttcagcg aagaaagagg ttgtaattgt gggctttgac ggcacagatg
      721 atggcgtaaa agccgttgaa agcggtaaac ttgccgcaac gattgctcaa caacctgaac
      781 tgattggctc attaggcgta gaaactgccg ataaagtgct aaaaggcgaa aaagtggaag
      841 caaaaattcc ggttgattta aaggttattt caaaataatt atacttgaag atagcgttcg
      901 tctctgacga acgctttgaa ttaataaaat agcactcgtc agagactagt gctatcagaa
      961 tcctagtttt gttgtactag gtaatttgag ggcttaatat gaaaaaactc accgttctcg
     1021 gtagcattaa cgctgatcac gtgatttccg ttccctattt tgccaaaccg ggggaaacgc
     1081 tcaccggaca tagctgccac attgcttatg gcggtaaagg ggcaaatcag gcggtggcgg
     1141 cagcacgttt gggagctaag gtttctttca tcggttgtat tggcagtgat gacatcggca
     1201 aagcaatgaa atcagccttt gagcaagatg gcattgacac tcgctcgatt aaaacgattc
     1261 cgaatgaaat gaccggcatt gcaatgattc aagtggcgga atcgggcgag aacagtattg
     1321 tgatttcagc cggagcgaac ggtcatttgg atgaaacggt tgtggcaaag tgtgcagccg
     1381 aaatcacaca ggcggattat ttgctaatgc agctagaaac accgttgccg gcgattgtgc
     1441 aagcagcaaa attggctcag caaaacggca cgaaagtaat attaaaccct gcgcctgctc
     1501 gagcgttacc cgatgaactg ttaaacttac tggatatgat taccccaaat gaaacggaag
     1561 cggaaatatt aaccggtgtg aaagttgttg atgaagccag tgcgattcaa gccgcacaag
     1621 tgtttcacca aaagggcatc gaaaaagtgt tgattacact tggctcaaaa ggggtatttg
     1681 taagcgaaaa cggtgtaggc aaaatcgttg caggcttccg tgttcaagcg gtggatacca
     1741 ctgccgcagg ggatactttt aacggagcat tagtgactgc aatgttagaa gataaaccgc
     1801 tggatcaagc cattcgtttt gctcacgcag cggcagcgat tagtgtgact cgcaaagggg
     1861 ctcaaccttc tattccgacc cgtcaggaaa cactggattt tctaagccaa caagcggtga
     1921 gtttttagga aaattttgca aatggcaaca atgaaggata ttgcacggct tgcacaggtt
     1981 tccacctcga ccgtttctca cgttatcaac aatagccgat ttgtcagcga ggaaattcgg
     2041 gaaagagtgc agagggtggt gaaagaactg aattacacgc cttctgcatt ggctcgtagc
     2101 ctcaaagtga atgaaactaa aaccatcgga atgctggtaa cggcaaccgg caatccgttt
     2161 tttgcggagg tgatggcggg ggtggagcaa tattgccagc aacatcaata taacctgatt
     2221 attgccacta ccaacggtga caccgagcgt ttgcaacacc atttgcaaat gctgatgcaa
     2281 cgaaaagtag atggcttgtt actgatgtgt ggcgatacca gattgaatgc cgatgagagc
     2341 ttgaatattc cgctgccatt ggtggtgtta gactggtggt ttaccgagct caatgcagat
     2401 aaaatctttg aaaattcaat atccggcggc tatctcgcaa caaaaacctt aattgaagca
     2461 gggcattcta agattgcgat tattactgga aacctaaaaa aatcattggc aaaaaatcgt
     2521 ttagaaggct acaaaaaagc acttgccgag gcgaatattc cactcaatcc aaactgggtg
     2581 ttcgaaagcc attttgattt tgccggcggg gttaatggaa tgaatgcttt gcttgcccaa
     2641 agcgaaaagc cgacagcggt atttgcctgt agcgacacca ttgctctagg ggcttaccaa
     2701 gcaatctggc aacaaggttt acgaataccg caagatattt cgattatcgg ctatgacgat
     2761 attgaactgg caaaatacct ttccccaccg ctgagtacca ttagccagcc caaagcggaa
     2821 ctcgggcggc ttgcggtgga aactttgcta aaacgaattc gttttaaatc cactgatttt
     2881 caaaccataa tgcttgaacc tgaattagtt ttaaggcatt caatccaaac aataaacaaa
     2941 aacgctgaat aatgtgaagt tattcagcgt tttttatccg atttcaatta gcctttttgc
     3001 tgttccaaat aaagcacggt ggcggcaact cgggaatgca cgtttaattt acgcagtaaa
     3061 ttgcggatat gcacttttac ggtttcttcg gaaataaaga gctgagccgc aatttgtttg
     3121 ttcgacatac cggttgcgag ccattgtaaa acatccagtt cacgatctgt taagcaactt
     3181 aaaggaccat tttctggctg gcggttgagt aaatgttggc gaacggtttc gcttaatacc
     3241 acttcaccgg cagccgcttt acggatatta tctagtagtt ccggggtgtc ggtatctttt
     3301 aataaatagc cgtccacacc agcatccatt aatgcaaata catcggattg ctcgtctgac
     3361 actgttaaaa caatgattct ggcatctata ccttgagcgc gcaatgagcg taaagtatct
     3421 agccctgaaa gccctttcat atttaaatcc agaataatca catccggctc aagtcgtaat
     3481 gcaagtttga taccgtcggt gccgttgctt gcttcaccca ctacactaaa ttggtgatct
     3541 aattccaata gttgacgcat tccctgacgc attaatgggt ggtcgtcaat gagtaaaatt
     3601 tgagttggag ctgtcattta tttctccttt cctttctctt aatcggttaa ttttgcaatt
     3661 attaattcaa aatagcaagt ttagggattt actaaatgcc gcttttttga tcttagctaa
     3721 tttttaacta aaaaagaaat tgcaaaactt tgcaagaaaa agaccgcttg ttcggctata
     3781 attactgcca atttttattt caagtgtgaa aagaatgaca aatcaaacaa cctattttgc
     3841 cacggcagcc cgtggttttg aggaaatact aaaaaccgaa ctggagcaga tttgtgcggc
     3901 agaatgcaaa gtcgctcaag gcggtgtgca ttttaccacc acgcaaaaag gggcttatca
     3961 ggcattatta cacagccgtt tagcctctcg gatcttactg ccgctcatca gcaccaaaat
     4021 ttttagtgat agcgatttat atgcttccat cgtgtcattt aactgggcgg atttattcga
     4081 tccccgtgat accttttaca ttgatttcag cggcactaac cgagaaatcc gcaacaccca
     4141 atttggggca atgcgagtga aagacggcat tgtggattac tttgaacgca aaggttttgc
     4201 ccgcccaaca gtagataaag ataatccgga tgttcgcatt catgtttatt tggatcgtga
     4261 aaatgttgtg gtttcgcttg atttaagtgg agatgcctta catattcggg gctatcggga
     4321 agaaaccggt aaagctccgt tgcgtgaaac attggcggca gccattattt tgcgttccgg
     4381 ctggaaagtc ggcacgccat tggttgatcc gatgtgtggt tcaggcacgt tactgattga
     4441 agcagcacaa atggcagcga atattgcccc tcaacttcac cgtaaacatt ggggctttaa
     4501 tgcgtggaaa gggcatcaac aagcgatgtg gaaagccgtg ttagatgagg cattccgaaa
     4561 tgttgaattg ggtgcggtag gggagaattg caattcgtcc ctacaaaaaa tgttcttcgg
     4621 ttttgatctc gatcatcgtg tgctgactaa agcaaagcaa aatgcgaaaa atgcaggggt
     4681 agatcatctc attcaatggc aacaaggtga tgtggctgcg ttaaaaaatc cgattccgga
     4741 aaaaatcggc acggtggtgt gtaacccccc ttatggtgaa cgtttaggca caacgccggc
     4801 gttaattgct ctttattcgg tgttcgggca acgcctaaaa cagcaatttg ccggttggaa
     4861 tgcctcaatt tttagcggcg agcctgaatt gttaaattgc ctgcgtttac gttcagcccg
     4921 tcaattcaaa gcgaaaaacg gtccgttgga ttgtgtgcag aaaaattact ccatcagcga
     4981 gcataacaag cggtcagatt tagatgaaaa tttacaattt gaacagaatg ctcaagtcgc
     5041 tccggatttt gcaaatcgct taaccaaaaa tatcaaaaag atcgaaaaat gggcgaaaca
     5101 acaaggcatt gatgcctacc gtttatacga tgccgatctg cctgaatata atttagcggt
     5161 ggacagatat ggcgaccaca ttgttgtgca ggaataccaa gccccgaaaa atattgatga
     5221 gcaaaaagcc cgccaacgct tgttagatgc agtctctgct acgctttatg tgacaggtgt
     5281 tgaaactaat aaattagtgt tgaaagttcg ccaaaaacaa aaaggcacga atcagtacga
     5341 aaaacttgcc aataaaggcg agtatttcta tgtgaacgag tatggggcga aactgtgggt
     5401 gaatttaacc gattacttgg atacgggttt gttcctagac catcgcttaa cccgtaaaat
     5461 ggtgggcgaa atggcaaaag gcaaaacatt cctgaatctg tttgcctaca cgggttcagc
     5521 tacggttcac gctgctctta atggggcaaa atcgaccact acagtggata tgtcgaacac
     5581 ctatttgaac tgggcggagc aaaatttaga gctgaataat ttatcacttc gccatcatcg
     5641 tttgttccaa gccgattgct tgcaatggct ggcagaatgc cgtgagcgat ttgagctgat
     5701 ttttgtcgat ccgccgactt tctccaactc aaaacgaatg gaaaacagtt gggacgtgca
     5761 acgagaccat attgagttaa tgaaacagct taaacgtatt ttaaccgctg atggtaccat
     5821 tgtgttctcg aacaataagc gtggctttaa aatggatttt gacggtttgg cagcattagg
     5881 cttaacggcg gaaaatattt cgcataaaac cttaccgttg gattttgaac gcaatccgca
     5941 aatccataat tgttggattg tgaagaatgt atagataata ccctcgaccc ttgtgggaga
     6001 gggatagatt tttcttcgtt cagaagaaaa atcagggaga gggtaagaag ttactgatga
     6061 aagagttatt tccccctctc tctgaaaaat atttctgaac gaaatatttt tcggtctctc
     6121 tcccacaagg ggagagagta aggctaaaaa atgttagata ttgttttata cgaacctgaa
     6181 attccgcaaa acagcggaaa tattatccgc ctttgtgcca actgtggctt tcgtttgcat
     6241 atgattgaac cgttcggctt tggttgggac gataaaaagc tccgccgttc ggggctggat
     6301 tatcacgaat ttgtggatat tcaaaaatat aaaaattttg aagatttcct tgagcgtgct
     6361 aagcctaaac gcttgttcgc cttaaccacc agaggggagc agaaccacag tgatgtgaaa
     6421 tatgaacttg gcgatttttt aatgttcggg ccggaaagca gaggcattcc aaagccatta
     6481 ttagcggaaa tgccgatgtc gcaaaaaatc cgcattccga tgtgcaaaga cagccgcagt
     6541 atgaatttat ctaactcggt ggcggtggtt gtgtatgagg cgtggcgaca attcggctac
     6601 caaaatgcgg tagcggcaca atctatttaa cttgcaagcg gtgtgatttt ggctattttt
     6661 tgcaagtttg tggtcaaggt tgggggaggc tcgaccttga ctaattaaga acatctattg
     6721 tgtctattct tcttcatttt tagctggagg taaaaaattc tctccggtaa caactgattt
     6781 acctgtttta ttctccaatt cctgacgggc atttttggca atattgccac cttgttttcc
     6841 tgcttgggca ttttcatcaa ccccaactgc ctgcatactt tctgcaattt gccgactgga
     6901 aagttcagca agtgctgtaa ataacaattc agcttcgctc atatgatctc gcagattttg
     6961 gctttttaaa cctttaagcg atttatgctt ttgtacagat agtccgctcc attcttgatg
     7021 aatcacattg gttaaaaatg caaaatcttg tggctgttca acccctgacg atttccaata
     7081 atcggtcagt ttatttcggg tttcttgtcc catcattctt tgctgaatcc atttttcact
     7141 tcgtcccatc ttctgccaat tctcccttgc tcgatctaga ctttgacttg gatcgtttat
     7201 ttcctgtaag cgttcataac ctacttttgc taaccattgt ttgaagggct ctgctttcgg
     7261 actagggata gattgaataa gtcggaaaag ttgttcggta tctgctacat cagtcaaacg
     7321 catttttccg tcagcagagg gcattttcaa cccgtgacaa tttgtcacgg tttcattgcc
     7381 ttctgccttt agcctctgtt ttagcttacg ccaataagca gagggatcta ggctttcggt
     7441 taaaatagca atcacatcaa taatagaaaa ataccattta tctttttctt catcgtaatg
     7501 actgcgtaca gatttctgtt caaaaagttt aatttcttgc ataataaaaa tactgtatat
     7561 aaaaacaaga aaagtataa
//
LOCUS       DANLYI010000113         7571 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.113, whole genome shotgun sequence.
ACCESSION   DANLYI010000113 DANLYI010000000
VERSION     DANLYI010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 7571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7571
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.113"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            28..1680
                     /locus_tag="PXD81_001682"
     CDS             28..1680
                     /locus_tag="PXD81_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HDL5537690.1"
                     /translation="MTIFQVAQNWLAQDPDAETRAELEQLIQAAESDEKAKAELTARF
                     DGRLQFGTAGLRGRLQAGSMGMNRVLVAQAASGLAGFIKGYDKEPSIVIGYDGRKNSD
                     VFARDTAEIMAAAGIKTYLLPRKLPTPVLAYAIQYFDTTAGVMVTASHNPPEDNGYKV
                     YLGKANGGGQIVSPADKEIAALIDKVAAGDIRDLPRSQDFTVLDDEVVNAYIEKTASL
                     AKRPKAEINYVYTAMHGVGYEVLSKTLEKAGLPQPYLVSEQIQPDGSFPTVNFPNPEE
                     KGALDLAIKLAKEKNAEFIIANDPDADRLAVAVPDAQGNWKPLHGNVIGCFLGWYLAK
                     QFHAQGKQGVLACSLVSSPALAEIAKKYGLSSEETLTGFKYIGKVENLLFGFEEALGY
                     LVDPDKVRDKDGISAAIMFLDLVCSLKQEGKTLADYTTEFVQEFGAYVSGQISIRVCD
                     LAEIGKLMTALRNNPPSEIGGFKVAEFIDHTKTTRQNDILVFVLENGSRLIARPSGTE
                     PKIKFYLDACGTDAENAEQVLAQFDESVRSLLRQEEYGKQDC"
     gene            1733..2667
                     /locus_tag="PXD81_001683"
                     /pseudo
     CDS             1733..2667
                     /locus_tag="PXD81_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250489.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
     gene            complement(2689..4770)
                     /gene="recG"
                     /locus_tag="PXD81_001684"
     CDS             complement(2689..4770)
                     /gene="recG"
                     /locus_tag="PXD81_001684"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009875323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HDL5537691.1"
                     /translation="MSEQLLDGVPLTALSGVGAAISEKLSRIGINNVQDLLFHLPMRY
                     EDRTRITPIADVRPESFATIEGYVQLTEVQFGKRPILSVTLSDSTSKIMLKFFNFNAG
                     MKNSLATGVRVKAFGEIKRGRFMAEIHHPEYQIIRDNQPLVLAETLTPIYSTTEGLKQ
                     ASLRKLTEQALALLEKVKVAELLPDEYNPHKYSLKEALQLLHRPPPTISAEVLEKGDH
                     PAQKRLIFEELLAHNLAMQQLRLGVKQQYAEALTYQTDIKQRFLDSLPFKPTHAQSRV
                     TADIEQDLAKPHPMMRLVQGDVGSGKTLVAALAALLAIDNGKQVALMAPTEILAEQHA
                     NNFANWLNPFGIEVGWLAGKVKGKARTAQLEAIKNGDVQMIIGTHALFQDNVEFHHLA
                     LVIIDEQHRFGVHQRLTLREKGAKENNYPHQLIMTATPIPRTLAMTVYADLDTSIIDE
                     LPPGRTPITTVVISEDRRAEIVQRVYQVCKQEHRQAYWVCTLIDESEVLEAQAAAAIA
                     EDLQRALPDLRIGLVHGRLKPQEKQAIMAEFKAANLDLLVATTVIEVGVDVPNASLMI
                     IENSERLGLAQLHQLRGRVGRGATASHCVLMYKPPLGKISSKRLQVMRDSQDGFYIAE
                     KDLEIRGTGEILGTKQTGMAEFKVADLMRDRKMIPLVQRYAKQIISENQPLAEALIKR
                     WLDNKAEYTNA"
     gene            complement(4774..5202)
                     /locus_tag="PXD81_001685"
     CDS             complement(4774..5202)
                     /locus_tag="PXD81_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HDL5537692.1"
                     /translation="MYRYLFDTNIVSELYKLRNDKMDVNVRQWLRGVNPSQTNISCIT
                     LSEIKTGILLKARKDKEQSDRLNDWFENNVLKAYQTKAFVVNNDIALLASEYHIPNKM
                     DLNDAYIAATAKYHNLILVTRNTKDFIRCDVRLFNPFETA"
     gene            complement(5202..5489)
                     /locus_tag="PXD81_001686"
     CDS             complement(5202..5489)
                     /locus_tag="PXD81_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HDL5537693.1"
                     /translation="MAITITSREFNQNASKVLKMAEKEPVFITKWGKIVNVVSSYMDY
                     QQKKPSEPSFEELFASSENEDVSDEFWQEFEQTLVEIRKSNKPRPVDFGDE"
     gene            5653..7536
                     /gene="htpG"
                     /locus_tag="PXD81_001687"
     CDS             5653..7536
                     /gene="htpG"
                     /locus_tag="PXD81_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009874843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HDL5537694.1"
                     /translation="MANNQETRGFQSEVKQLLQLMIHSLYSNKEIFLRELISNASDAA
                     DKLRFKALSEPALYEGDGELRVRVSVDETLGTITIADNGIGMTREQVIDHLGTIAKSG
                     TKEFLNALGTDQAKDSQLIGQFGVGFYSSFIVADKVTVKTRAAGVPAEQGVIWESAGE
                     GDYTVADLEKKERGTEIILHLREDEKEFLNEWRLREIIGKYSDHIGLAVEIQTNEYDD
                     EGKASGQKWEKINKAQALWTRSKNEISDEEYTEFYKHLSHDFADPLIWSHNKVEGKQE
                     YTSLLYVPSKAPWDLFQREQKHGLKLYVQRVFIMDDAEVFMPNYLRFMRGLLDTNDLP
                     LNVSREILQENKVTQSLRSALTKRALQLLEKLAKDDAEKYAQFWQAFGVVLKEGVGED
                     FANKQQVAGLLRFASTQRDDSEQAVSFADYIARMKEGQKAIYFLTADSYQAAKNSPHL
                     ELFHKKGIEVLLLSDRIDEWLVSHLTEFDGKPLQSITKSDLDLGELADKQEEESQKAQ
                     QAEFGSFLERAKGYFGERVKNVVLTHRLTDTPAVVSTDSDEMTTQMAKLFAAMGQQAP
                     EVKYTFELNPDHPMVKKVADLADEAEFADWIELLFEQALLAERGTLENPTAFIKRVNK
                     LLG"
BASE COUNT         2041 a         1676 c         1812 g         2042 t
ORIGIN      
        1 agtttttaaa ttacatagga gtaaaaaatg actattttcc aagttgcaca aaattggtta
       61 gcccaagacc cggatgcaga gacccgtgcg gaattagagc agttaattca agcggcagaa
      121 tcggacgaga aagcaaaagc agaattaaca gcccgttttg acggacgttt acagtttggt
      181 acggctggtt tgcgtggtcg cttacaagcg ggttcaatgg gtatgaaccg tgttttggtg
      241 gcacaagctg ccagcggttt agctgggttt attaaaggtt atgataaaga gccgtcaatt
      301 gttattggtt atgacggtcg taaaaattcc gatgtgtttg cccgtgacac cgctgaaatt
      361 atggcagcgg caggcattaa aacgtacctg cttcctcgca aattgccaac accagtgctt
      421 gcctatgcga ttcaatattt tgataccaca gccggtgtga tggtaactgc cagccacaac
      481 ccaccggaag ataacggcta taaagtttat ttaggtaaag ccaacggtgg cggacaaatt
      541 gtttcgccgg cagataaaga aattgctgct ctgattgata aagtagcagc aggcgatatt
      601 cgtgatttac ctcgtagcca agatttcacg gtgttagacg atgaagtggt aaatgcttat
      661 attgagaaaa ccgcctcact agccaaacgg ccaaaagccg aaataaacta cgtttacact
      721 gcaatgcacg gtgtgggcta tgaagtgtta agcaaaacat tagaaaaagc cggcttaccg
      781 caaccgtatt tagttagtga gcagattcag ccggacggtt cgttcccaac ggttaatttc
      841 ccgaacccgg aagagaaagg ggcgttagat ttagcgatta aattggcgaa agagaaaaat
      901 gccgaattta ttatcgccaa cgacccggat gctgaccgtt tagcggtagc cgtgcctgat
      961 gctcaaggca actggaaacc actgcatggc aatgtgatcg gctgtttctt aggctggtat
     1021 ttagccaaac aattccacgc acaaggtaaa caaggcgtgt tggcttgctc gttagtatcc
     1081 tctccggcgt tagctgaaat tgcgaaaaaa tatggtttaa gctcggaaga aaccttaacc
     1141 ggctttaaat atatcggaaa agttgaaaac ctattatttg gctttgaaga agccttaggt
     1201 tatttggttg acccagacaa agtgcgtgat aaagacggca tttccgctgc cattatgttc
     1261 ttagatttag tgtgcagcct caaacaagaa ggtaaaaccc ttgcggatta caccaccgag
     1321 tttgtacaag agttcggggc ttatgtaagc ggtcaaattt cgatcagagt ttgtgattta
     1381 gccgaaatcg gcaaattaat gacggcgtta cgcaacaatc ctccaagcga aattggtggc
     1441 tttaaagtgg ctgagtttat tgaccatacc aaaaccacac gccaaaatga cattttagtg
     1501 tttgtgttgg aaaatggcag ccgtttgatt gctcgccctt caggtactga gccgaaaatt
     1561 aaattctatt tagatgcttg tggcacagac gctgaaaacg cagagcaagt gctggctcag
     1621 tttgacgaaa gtgtgcgttc gctgcttcgc caagaagaat acggcaaaca agattgctaa
     1681 tggcattaat tgagggggag cttaggctct ccctttttta aaggagacaa taatggctca
     1741 aaaaattatt ttagattgtg atccggggca tgacgatgcg attgcgatga tgctcgcttg
     1801 ggggaatccg aatattgatt tgttggcagt gactacggtg gtaggtaatc aaaccttaga
     1861 aaaagtatcg cgtaatgcgt tggcggttgc ggaaattgct catatttgca acgttccttt
     1921 tgccaaaggt tgtgccagac cgctggtgcg tgaagtggaa aatgccccgg atattcacgg
     1981 tgattcggga atggatgggc ctgtactccc tgaatctacc cgacaatttg agaaaaaaca
     2041 cgccgctcaa ttaattattg atttgataat gtcgcacccg gagaaaagca ttaccttagt
     2101 gccgaccggt gggcaataac caatattgct ctagctgccc gccttgagcc ccgtattatt
     2161 gaaagagtga aagaagtggt gctgatgggc ggaggttatc acaccggcaa ctggagtgcg
     2221 gtggcagaat ttaatattaa aattgacccg gaagctgctc atattgtgtt taatgccggt
     2281 tggaaggtga cgatggtcgg cttagactta actcaccaag cacttgcgac ccccgatgtg
     2341 gttgaacgct ttaaggcatt aaacagcaaa tcggggcagt ttgtggtgga attgctggcg
     2401 ttcttcgcca aaatgtataa acaggctcaa ggctttgatg ctccaccggt tcacgatgcc
     2461 tgtgcggtgg cgtatgtgat tgacccaagc ctgattgaag ttcagcaagt gccggtcaat
     2521 attgagctaa caggcacgca tacactgggt atgacggtgg cggatttccg ctacccgcca
     2581 aaacagtgta atacttgggt agcaaaaaaa ttagacaaat cccgcttttg ggatttggta
     2641 attgaatcgg tgaaaaacct gccgtaatct aaataacccg cttgccggtt aagcgttggt
     2701 atattccgcc ttgttgtcta accaacgctt aattaacgcc tctgcaagcg gttgattttc
     2761 gctaataatt tgcttggcgt aacgttgcac cagcggaatc attttgcggt ctcgcattaa
     2821 atccgccacc ttaaattctg ccattccggt ctgttttgtg ccgagaattt cgcctgtgcc
     2881 tcgaatctcc aaatcttttt cggcaatgta gaaaccgtct tggctatcac gcatcacttg
     2941 taaccgcttg ctggaaattt tcccgagcgg cggtttgtac attaagacgc aatgcgaggc
     3001 ggttgcccct cgcccaacac gtccacgcaa ctggtggagt tgagccagcc cgagtcgttc
     3061 ggaattttcg ataatcatta ggctagcatt cggcacgtcc actcccactt caattacggt
     3121 ggtcgcaacc agcaaatcta aattcgctgc cttaaattcc gccataatcg cttgtttttc
     3181 ctgtggtttc agccgtccgt gtactaaccc gatgcgtaaa tccggcaagg ctcgctgtaa
     3241 atcttcggca atcgctgccg cggcttgagc ctccaacact tcgctttcat caatcagggt
     3301 gcagacccaa taggcttggc ggtgttcttg cttacagact tggtacaccc gttggacgat
     3361 ttctgctcgc cgatcttcag aaatcaccac agtcgtgatt ggcgttctac ccggtggcag
     3421 ttcgtcaatg attgaggtgt cgaggtcagc atagaccgtc attgccagcg ttctcggaat
     3481 gggggtcgcc gtcataatga gctgatgcgg atagttattt tctttcgctc ctttttcccg
     3541 caaggtcagc cgttggtgta cgccaaaacg gtgttgttca tcaataatca ccaacgctaa
     3601 atggtgaaat tccacattgt cttggaacag ggcgtgagtg ccgataatca tctgcacatc
     3661 accgtttttg attgcctcaa gttgtgcggt tctcgccttg cctttcactt taccggcaag
     3721 ccagccaacc tcaatcccaa agggattaag ccagttggca aaattattgg cgtgctgttc
     3781 ggcaagaatt tcggtcggtg ccattaatgc cacctgcttg ccgttatcaa tcgctaatag
     3841 tgctgccaag gcggctacta aggttttacc tgaaccaaca tcgccttgta ccagtcgcat
     3901 catcgggtgt ggtttggcaa gatcttgctc aatatctgcg gttactcggc tttgtgcgtg
     3961 agtaggctta aaaggcaaac tgtcgagaaa acgctgtttt atatcggttt ggtaagtaag
     4021 tgcctctgca tattgctgtt ttacccctaa acggagttgc tgcatcgcta aattgtgtgc
     4081 cagcagttct tcaaaaatca gccgtttttg agcagggtgg tcaccttttt ccaagacttc
     4141 ggcagaaatc gtcggcggtg ggcggtgcaa taactgcaac gcttctttta ggctgtattt
     4201 gtgcggattg tattcatcag gcaacagctc ggcgactttc actttctcta ataaagcgag
     4261 ggcttgctcg gttaatttcc gcagcgaggc ttgttttaac ccctcagtgg tggagtaaat
     4321 cggggtcagg gtttcagcca gtacaagcgg ttgattatcc cgaataattt gatattcagg
     4381 gtggtgaatt tccgccataa atcgcccacg cttaatttcc ccaaaggctt ttacccgaac
     4441 gccggtggca aggctatttt tcattccagc attgaaattg aagaatttaa gcataatttt
     4501 gctggtgctg tcggagagtg taaccgataa aatcgggcgt ttaccaaact ggacttcggt
     4561 aagttgtaca tagccttcaa tggtggcaaa gctttccggg cgaacatcag caatcggggt
     4621 aatacgggtg cgatcttcat accgcattgg cagatgaaac agtaaatcct gcacattatt
     4681 aatgccgatt cgacttaact tttcggaaat tgccgctcct acgccggaaa gggcggttaa
     4741 tggcacgccg tctaataatt gttcactcat tatttatgcg gtttcaaatg ggttaaataa
     4801 acgcacatca caacggataa aatctttggt gttgcgggtg actaaaatca gattgtggta
     4861 tttggcggtt gccgcaatat aagcatcatt taaatccatt ttattgggaa tatggtactc
     4921 tgatgccaat agagcaatgt cgttattcac cacaaacgct tttgtttgat aagcctttaa
     4981 tacattattt tcaaaccaat catttaatcg gtctgattgt tctttgtctt tgcgggcttt
     5041 cagcaaaata cctgttttga tttcagataa cgtaatacag ctgatattgg tctggctagg
     5101 attaacccct ctcagccact ggcgaacatt gacatccatc ttgtcatttc ttaacttata
     5161 aagctcggaa actatattgg tatcaaaaag gtagcgatac attactcatc tccaaaatca
     5221 acaggtcttg gtttattgct tttgcggatt tcaaccaatg tttgttcaaa ctcttgccaa
     5281 aattcatcgc tcacatcctc attttcggaa gaggcgaata gctcttcaaa gcttggctcg
     5341 ctaggctttt tctgctgata atccatataa ctagacacca cattgacgat tttgccccat
     5401 ttggtaataa aaaccggctc tttttctgcc atttttaaca ctttgctggc attttgatta
     5461 aattctctgg aagtaatggt tattgccata tcgctctcca aaatgtagta aaaagatgag
     5521 tgtattttat taaatgtagt aatatctttc aattaattcg ctatttggca acagatattt
     5581 tgcaaaaaat cttgaaaatc tgaccacttg ccccatctat tccctgtttt ttaatgaata
     5641 aaggaataca caatggcaaa taatcaagaa acccgtggct tccagtcgga agtgaaacag
     5701 ttattgcaac tgatgatcca ctcgctttac tccaacaaag aaattttcct gcgtgagctt
     5761 atttccaacg cctctgacgc cgccgataaa ttgcggttta aagccctttc cgagccggct
     5821 ttgtatgaag gtgatggcga actgcgtgtg cgtgtctctg ttgatgaaac actcggtacg
     5881 attacgattg ccgataacgg tatcggtatg acccgtgagc aggtgatcga ccatctcggt
     5941 acgattgcaa aatctggtac caaagaattt ttgaacgcac tcggcactga tcaagccaaa
     6001 gacagccagc ttatcgggca gttcggcgtg ggcttttact cttctttcat cgtggcggat
     6061 aaagtgacag tcaaaacccg tgccgccgga gtgcctgccg agcaaggcgt gatttgggaa
     6121 tctgccggtg agggcgatta caccgttgcc gatctggaga aaaaagagcg tggcacggaa
     6181 atcattcttc acttgcgtga ggacgaaaaa gagtttttaa acgaatggcg tttgcgtgag
     6241 attatcggca aatactccga ccatatcggc ctggcggtgg aaattcagac caatgaatac
     6301 gatgacgagg gcaaagcaag cggtcaaaaa tgggaaaaaa ttaacaaagc tcaagcgctt
     6361 tggacacgct caaaaaatga gattagtgac gaggagtaca ccgaatttta caaacacctc
     6421 agccatgatt ttgccgaccc gctgatttgg tcgcacaata aagtcgaagg taagcaagaa
     6481 tacaccagct tgctgtatgt gccgagcaaa gccccgtggg atctgttcca acgcgagcaa
     6541 aaacacggct tgaaactcta cgttcagcgt gtatttatta tggacgatgc ggaagtcttt
     6601 atgccgaact acttgcgttt tatgcgtggc ttgctcgaca ccaacgacct gccgctcaac
     6661 gtcagccgtg aaattctgca agagaataaa gttacccaat cgctccgttc tgccttaacc
     6721 aagcgtgcgt tacaactgct ggaaaaattg gcgaaagacg atgccgaaaa atatgcccaa
     6781 ttctggcaag cgttcggcgt ggtgcttaaa gagggcgtgg gcgaagactt tgccaacaaa
     6841 caacaggttg caggcttact ccgttttgcc tccacccagc gtgatgacag cgaacaagcg
     6901 gtcagttttg ccgattatat tgcaagaatg aaagaggggc agaaagcgat ctacttctta
     6961 accgcagaca gctaccaagc ggctaaaaac agcccgcact tagagctgtt ccacaaaaaa
     7021 ggcattgagg tgctactgct ttctgaccgc attgatgaat ggttggttag ccacctcacc
     7081 gagtttgacg gcaaaccgct acaaagcatt accaagtcgg acttagactt aggcgagctt
     7141 gccgacaagc aggaagaaga gagccaaaaa gcccaacagg cggagtttgg ttcgttctta
     7201 gaacgggcga aaggctactt tggtgaacgg gtcaaaaacg tggtgctgac tcaccgctta
     7261 accgacacac cggcagtggt ttccactgac agcgatgaaa tgaccaccca aatggcaaaa
     7321 ctgtttgcag caatggggca acaagcacca gaagtgaaat atacttttga gctgaaccca
     7381 gatcacccaa tggtgaaaaa agtagctgat cttgccgatg aagccgagtt cgccgattgg
     7441 attgaactgc tgtttgaaca agctctgctt gctgaacgag gcacgttgga aaacccgaca
     7501 gcctttatta aacgggtaaa taaattgttg ggctagccat tactaaaaac ggaaatctag
     7561 gtttccgttt t
//
LOCUS       DANLYI010000114         7545 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.114, whole genome shotgun sequence.
ACCESSION   DANLYI010000114 DANLYI010000000
VERSION     DANLYI010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 7545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7545
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.114"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            20..592
                     /locus_tag="PXD81_001688"
     CDS             20..592
                     /locus_tag="PXD81_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDL5537695.1"
                     /translation="MSRRSEPKEEMVNRILSATESLIVQEGLQNLSMRSIAKEAGIAS
                     GTLYLYFKTKDDLLHSLAAQLLECYNGYMHFEFKPEMALFEQYKALVNKKWQFLIERL
                     DLVKQFQAVIGIDELIRETIADETSFWNKLILEGKKQRIIADLSNELLYGLSIGTLVD
                     IQYLQNLNQENQFDGYLDEIILRTWKAITF"
     gene            623..1828
                     /locus_tag="PXD81_001689"
     CDS             623..1828
                     /locus_tag="PXD81_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005614807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HDL5537696.1"
                     /translation="MTTETQKLGKGRKFLIVLTLLIVLIVFAAIVGLQKIGAMKKAEA
                     AANMPEAVSEVTAMQVTTQEWTPTISAVGYIRPNQGAMLSAETSGVVSRVLVTSGQRV
                     KKGDLLVEFDSSVEIANLRASEAQLPNAKANLERYRNLIASNSASKAEFDNAQSTYNQ
                     LLANIEALRATIKRRQIYAPFSGLAGIVNVNVGQYITMGTEIVRVEDQSSMKVRFTLP
                     QTNLEQISIGQKVTAVIDALPAQTFPAKIVAIDPAVDRLTGLINVEAVIEEGQEKLLS
                     GMFARLNVALPTETGQIVVPQISIAYTMYGETVYVLQPLSDEDKEMVNKMAVQNPSLD
                     VNKMYRAKQMEVKTADRKGNYSQLSKGVKAGDLIVTGGLQRLSNNALVKVSDTEAVGI
                     TTPAKNSKL"
     gene            1842..5009
                     /locus_tag="PXD81_001690"
     CDS             1842..5009
                     /locus_tag="PXD81_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015587060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HDL5537697.1"
                     /translation="MKFTDIFIKRPVLAVCISLLITILGLQAIEKLQVREYPEMTVSI
                     VTVSVNYSGADARLMQTLVTSQLEEAVAQADNIDYMTSSSSPSTTTVTAKMKLNTDPN
                     AALSDISAKVNAVRSNLPSGIDDPSIRVSTGSNDALMYIRFVSDELNTSQVTDYINRV
                     VKPQFFTVNGVSSVDVYGSVFGLRIWLDPDKMASNNLSGAAVLSALSANNLQTAAGNS
                     NGYYTVYRNKVLSTTPSVTELEEVTVSTTKDGKTIKLKDIATVELDKYQDAARATVGG
                     KDAVVLAVSVAATANSLTVAKDIYPMFEQVLKNLPSTIQGDIVYDKTIAIDSSIKEVI
                     KTIGEATVIVLVVIILFLGSLRAMIVPVITIPISLIGVLFFLQMFGFSINLLTLLALV
                     LAIGLVVDDAIVVLENVERHVKEGKTPFDAAIIGTREIALPVISMTITLGAVYSPMAL
                     MTGVTGTLFKEFALTLAGAVFISGIAALTLSPMMSSRILKEHKEGEESAFARVVHRSL
                     DKMTSCYTNMLSGIMAVRGFVLFFAVLIFASLPMLFGSLSSEVAPTEDRGFVVGISNG
                     PSNTNLDYTEAALAQFNQEVTKLPEVSSLMTVSGFNGANSALSIIALKDWNDRSRERA
                     AISNDVAAIGKKVVGMDVNAIAFPEISTGENDLPFSLVITTADSYEKLAIVASEFLKK
                     AQTSGQFFFANLDLKFDTVTMNMKFDREKMGAYGVTMQQVSGTLGAFLSGAIITRVDI
                     DSRAYPIVSQAVRNDRLNPEDLMNYYVTTATGQSIPLGSFVTMELKTSPSSLPRMSQL
                     NSATIGGVVSGSIGDAVNWAKAELDRTLPSGYQYDFRGESRQFVQEGNAMAMTFALAV
                     IIIYLVLAIQFESWRDPLVILVSVPLAVSGALLVMNIPTAMKGLFMGIAAGTGSKDMM
                     AFANGIWTAGYTLNIYSQVGLITLVGLITKHGILMCEVAKEEQLLHGKSRYEAIFHAA
                     TIRLRPIMMTTAAMIAGLIPLLFAVGAGAIARFSMGMVIVAGLAIGTLFTLFILPVIY
                     TFLGQEHKPLREFDEEKYFPTTKEA"
     gene            5237..6700
                     /gene="guaB"
                     /locus_tag="PXD81_001691"
     CDS             5237..6700
                     /gene="guaB"
                     /locus_tag="PXD81_001691"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005725353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HDL5537698.1"
                     /translation="MLRIKQEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTETKLAISLAQEGGIGFIHKNMSIERQADRVRKVKKFESGIVSEPVTISP
                     DMTLAELAELVKKNGFAGYPVIDENQNLVGIITGRDTRFVTDLSKTVREFMTPKDRLV
                     TVKENASREEIFHLMHEHRVEKVLVVNNEFQLKGMITLKDYQKAESKPNACKDEFGRL
                     RVGAAVGAGPGNEERIDALVKAGVDVLLIDSSHGHSEGVLQRVRETRAKYPDLPIVAG
                     NIATAEGAIALADAGASAVKVGIGPGSICTTRIVTGVGVPQITAIAEAAAALKERGIP
                     VIADGGIRYSGDISKAIAAGASCVMVGSMFAGTEEAPGEIELYQGRAFKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRIAYKGYLKEIIHQQMGGLRSCMGLTGCATI
                     EELRTKAEFVRISGAGIKESHVHDVTITKEAPNYRMG"
     gene            6821..7492
                     /locus_tag="PXD81_001692"
     CDS             6821..7492
                     /locus_tag="PXD81_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HDL5537699.1"
                     /translation="MLQAIIFDMDGVIVDTEYLEFSLQKQFIEDIKEHSRPITLEQQS
                     EVVGKCLKEIPVIIKKLSESSLPIEEIRARYYAFFQDLFSKVDFKTIFRADIQQIIQF
                     AKQNQIKLAVASSSALSHIENILTVCGIKDEFDLIVSGEQFEHSKPDPTIYRYTCEKL
                     GVEPQNAVAIEDSYYGMLAAKTAGLTVIGYEEKRILIDQSLADYMGKDMKEILKILQK
                     CNTPS"
BASE COUNT         2246 a         1414 c         1720 g         2165 t
ORIGIN      
        1 ttataggtaa ggagataaaa tgtctaggcg aagcgagcca aaagaagaga tggtaaaccg
       61 tattttatcg gcaaccgagt cgctgattgt gcaagaaggt ttgcaaaacc tttcaatgcg
      121 tagtattgca aaagaagcag ggatcgcttc cggtacatta tatctttact ttaaaaccaa
      181 agatgattta ttgcactcat tggctgccca attactcgaa tgttataacg gttatatgca
      241 ttttgagttt aagcctgaaa tggcgttatt tgagcaatat aaagcgttgg tcaataaaaa
      301 atggcagttt ttaatcgagc gtttagattt ggtaaaacag tttcaagcgg tgatagggat
      361 tgatgaattg attcgggaaa ccatagctga tgaaacttcc ttttggaata aattaatttt
      421 agaagggaaa aagcaaagga ttattgctga tctatctaat gagttgcttt atggattaag
      481 catcggcaca ttggtggata tccaatatct gcaaaatctg aatcaagaaa atcagtttga
      541 tggatattta gacgagatta ttttacgcac ttggaaagca atcacttttt aagtttcttt
      601 ttattagttt tatggagaat gtatgacaac ggaaacacag aagctgggta aaggcagaaa
      661 gtttttaatt gtactcacat tgttaatcgt attgattgtg tttgctgcaa tcgtagggtt
      721 acaaaaaatc ggggcaatga aaaaagccga agcagcggca aatatgccgg aggcggtgag
      781 cgaagttact gctatgcaag tgacgaccca agaatggaca ccaaccattt ctgcggtcgg
      841 ttatattcgc ccaaatcaag gggcaatgtt aagtgctgaa acttcaggcg tggttagccg
      901 tgttttggtc acttcaggac agcgtgtgaa aaaaggtgat ctgttagtag aatttgacag
      961 cagtgttgaa attgctaact tgagagcctc tgaagcgcaa ttaccgaatg ccaaagcaaa
     1021 tttagagcgt taccgtaatc tgattgcctc aaacagtgct tctaaagccg agtttgataa
     1081 tgctcaatct acctacaacc aattattagc caatatcgag gcattgcgtg ctacgattaa
     1141 acgccgtcaa atttatgctc cgtttagtgg tttagcgggt atcgtgaatg tgaatgttgg
     1201 gcaatacatt acaatgggta cggaaattgt gcgtgtggaa gatcaatctt caatgaaagt
     1261 acgttttacc ttgccgcaaa ctaatttaga gcaaatctcg attgggcaaa aagtgacagc
     1321 cgtaattgat gcgttaccgg cacaaacctt cccagctaaa attgtggcta ttgatccggc
     1381 tgtggatcgt ttaaccggct taattaatgt ggaagcggtg attgaagaag ggcaggaaaa
     1441 attgctttca ggtatgtttg cccgcttaaa tgttgcttta ccaacagaaa ccggacaaat
     1501 cgtcgtacca caaatttcta tcgcttacac tatgtatggt gaaacggtat atgtgctgca
     1561 accattatct gatgaagata aagaaatggt gaacaaaatg gcggtacaaa acccaagttt
     1621 agatgtaaac aaaatgtacc gtgcaaaaca gatggaagtg aaaacggctg accgcaaagg
     1681 aaactattca caattatcca aaggcgtgaa agcaggtgat ctgattgtaa caggcggttt
     1741 acaacgttta agtaataatg cgttggttaa agtttctgat acagaagccg taggcattac
     1801 cacaccggcg aaaaacagta aactgtagtg gagtgagtta agtgaaattt actgacatct
     1861 ttattaaacg tcctgttttg gcagtttgta tcagcctatt gattacgatc ctaggcttgc
     1921 aagcaatcga aaaattgcag gtgcgtgaat atccggagat gacggtctcg attgtaaccg
     1981 tgagtgttaa ctattcaggt gcagatgcac gtttaatgca gacactggtt acctcacagt
     2041 tagaagaggc tgttgctcaa gcggataata ttgactatat gacttcatca agctctccaa
     2101 gtaccactac ggtaacggca aaaatgaagt taaataccga tccgaatgcc gcactttccg
     2161 atatttcggc aaaagtgaat gcggtacgct caaatttacc gagcggtatt gatgatccgt
     2221 caattagagt ctcaaccggt tcaaatgatg cgttaatgta tatccgattt gtctctgatg
     2281 aacttaatac atcacaagtt accgattata ttaaccgtgt agtaaaacca cagttcttta
     2341 cggtaaatgg tgtttctagc gttgatgtgt atggctctgt ttttggctta cgcatctggc
     2401 ttgatccgga taaaatggcg agcaataacc tatcaggggc ggctgtatta tcggcattaa
     2461 gtgccaataa cttgcaaacc gctgcgggta attctaacgg ttactatacg gtttatagaa
     2521 acaaagtact ttctaccacg ccttctgtga cagagttaga agaggttacg gtttcaacca
     2581 ccaaagatgg aaaaaccatc aaattaaaag atattgcgac tgttgagctg gataaatacc
     2641 aagatgcagc aagagcaacg gttggcggta aagatgcagt tgtgcttgct gtttcggtag
     2701 ccgcaacggc aaactcttta accgtagcga aagatattta tccgatgttt gagcaggtat
     2761 taaaaaatct accaagcacc attcaaggag atattgttta cgataaaacc attgcgattg
     2821 atagttctat caaagaagta attaaaacta ttggtgaggc aaccgtcatt gtattggtag
     2881 taattatcct gttcttaggt tcattacgag caatgattgt accggtgatt accattccga
     2941 tttccttaat cggggtatta ttcttcctgc aaatgtttgg tttctcgatc aacttgctta
     3001 ctttgctggc gttggtatta gcgatcggct tggtagtgga cgatgcgatt gtcgtacttg
     3061 aaaacgtaga acgtcacgtt aaagaaggta aaacgccatt tgatgcagcg attatcggta
     3121 cacgtgaaat tgctttaccg gttatttcaa tgactattac tctgggtgca gtatattcac
     3181 caatggcatt aatgaccggg gtaacaggga cactatttaa agagttcgct ttaacgcttg
     3241 ccggtgcggt atttatttcg gggattgcgg cattaaccct ttcgccgatg atgtctagcc
     3301 gaatattaaa agaacataaa gaaggggaag agtcagcatt tgcccgcgtg gtgcatagat
     3361 cactagataa aatgaccagc tgctatacca atatgctaag cggtattatg gctgttcgtg
     3421 gtttcgtctt attctttgcg gtgctgatct tcgccagttt accgatgtta tttggctcac
     3481 tttccagtga agttgcaccg acagaagacc gtggctttgt ggttggtatt tctaatggac
     3541 cttcaaacac caatttagat tataccgaag cagcgttagc ccaatttaat caggaagtaa
     3601 ctaaattacc tgaagtatct tccttaatga cagtttcagg ctttaatggt gcaaatagtg
     3661 cattatcgat tattgcatta aaagattgga atgatcgtag ccgtgaacgt gctgcgattt
     3721 ctaatgatgt ggctgcaatc ggtaaaaaag tggtgggtat ggacgtgaat gcaattgcat
     3781 tccctgaaat ttcaaccggt gaaaatgatc taccattctc attagtgatt actaccgctg
     3841 atagctatga aaaattggca atcgttgcct ctgaattctt gaaaaaagcc caaacttccg
     3901 gtcagttctt ctttgcgaat ttagatttga aatttgatac ggtcacgatg aatatgaaat
     3961 tcgaccgtga aaaaatgggt gcttatggcg tgacaatgca gcaggtgagt ggcacattag
     4021 gggcattctt atccggtgcg attattactc gtgtcgatat tgactctcgt gcttacccga
     4081 ttgtgtcgca agcggtgcgt aatgatcgtt taaatcctga agatttaatg aattactatg
     4141 tcacgactgc aacagggcaa tctatcccgt taggttcatt tgtgacgatg gaattaaaaa
     4201 catcaccatc ttctttacct cgtatgagcc aattaaactc ggccaccatt ggtggggtag
     4261 tatctggctc aatcggcgat gcggtaaatt gggcgaaagc agaattagat cgcactttac
     4321 catcaggtta tcaatatgac ttccgtggtg aatctcgcca gttcgttcaa gaaggtaatg
     4381 cgatggcaat gactttcgct ctagcagtta tcattatcta tcttgtactt gcaattcaat
     4441 ttgaatcttg gcgtgaccca ttagtaattt tggtttctgt gccgttagct gtgagtggtg
     4501 cattgttggt aatgaacatt ccaaccgcaa tgaaaggctt gtttatgggg attgcagcag
     4561 gcactggctc gaaagatatg atggcattcg ccaacggtat ttggacggca ggttatacct
     4621 taaatatcta ttcacaagta ggtctaatta cccttgtcgg cttaattact aaacacggta
     4681 tcttgatgtg tgaagtggcg aaagaagagc agttactgca cggcaaatca cgctatgaag
     4741 ctattttcca cgcagccacg attcgtttac gcccaattat gatgacaacg gcagcaatga
     4801 ttgcaggctt aattccgctt ctgtttgcgg tgggtgccgg tgcaatcgcc cgcttcagta
     4861 tgggtatggt aatcgtagca ggcttggcaa tcgggacatt gtttactctg tttatcttgc
     4921 cggttatcta cactttctta gggcaagaac acaagccatt acgtgaattt gacgaggaaa
     4981 aatatttccc aacgacaaaa gaagcatagt tttaaatagc aaaagagcgt agcgatacgc
     5041 tcttttttgt tggcttacaa gcggtcgttt tttatttatt gtttgctaaa aaatcacaaa
     5101 atttaaccgc ttgaaagtat attttcagat agcgtaaacg tttccctatt tacagaaacc
     5161 tagttcctct gtataatccg ctcgcaattt tccagtacag attttcaatt taatcatttt
     5221 ttaaggaata attgcaatgc tacgaattaa acaagaagcc ctcacttttg atgatgttct
     5281 tctcgtcccg gcacattcta ctgtgcttcc taatactgct gatctttcta ctcaattaac
     5341 taaaaccatt cgtttaaaca ttccgatgct ttctgctgca atggataccg ttacagaaac
     5401 taagcttgcg atctcccttg ctcaagaagg cggcattggt tttatccata aaaatatgtc
     5461 gattgaacgc caggcagacc gtgtgcgtaa agtgaaaaaa tttgaaagtg gtattgtttc
     5521 tgagccagtg acgatttctc ctgatatgac attagcggaa ttggctgaat tggtgaaaaa
     5581 gaacggtttt gcaggctatc cggtgattga tgaaaaccaa aatttagtgg gaattattac
     5641 cggacgtgat acccgatttg tcacggattt aagcaaaaca gtgcgtgaat ttatgacacc
     5701 aaaagaccgt ttagtgacgg taaaagaaaa cgcaagccgt gaagaaattt tccacttaat
     5761 gcacgaacac cgagtggaga aagtgctggt agtgaataat gaatttcagt taaaaggaat
     5821 gattacccta aaagactacc aaaaagcgga aagcaaaccg aatgcctgta aagatgagtt
     5881 tgggcgtttg cgtgtggggg cggcagtggg agccggtccg ggcaatgaag aacgaattga
     5941 tgctttagta aaagcgggtg tcgatgtgct attaatcgac tcttcgcacg ggcattctga
     6001 aggtgtatta caacgtgtgc gtgaaacccg tgcaaaatac cctgatttac cgattgttgc
     6061 cggtaatatt gccactgcag aaggagcgat tgcgttagct gatgcaggag ccagtgctgt
     6121 gaaagtagga atcggcccgg gttcaatttg taccaccaga attgtaacag gcgttggcgt
     6181 gccacaaatc acggcaatcg cagaagcggc agctgcgctt aaagaacgag gcattcctgt
     6241 gattgctgat ggtggaattc gttattcagg cgatatttca aaagctattg ccgccggtgc
     6301 aagttgcgta atggtcggtt cgatgtttgc cggcacagaa gaagccccgg gtgaaattga
     6361 gctttatcaa ggcagagcat tcaaatccta ccgtggaatg ggatcattag gtgcaatgag
     6421 taaaggctcg tcagatcgct atttccaatc tgataatgcc gccgacaagc tcgtaccgga
     6481 agggattgaa gggcgtatcg cttacaaagg ctacttgaaa gaaattatcc accaacaaat
     6541 gggcggctta cgctcctgta tgggattaac cggctgtgcc actattgaag aactccgcac
     6601 caaagcagaa tttgtccgca ttagtggtgc tggtattaaa gaaagccacg tccacgatgt
     6661 gacaattacc aaagaagcac cgaactaccg aatgggttaa tagcctttct cactctctcc
     6721 ccttgtggga gagagaccgt tttaaattac gttcagtaat ttaaaacaga gagagggaat
     6781 aagtggttag attttcttaa aattttacaa aagaggctat atgctccaag ctatcatttt
     6841 tgatatggac ggcgtgattg tggataccga atatctggaa ttttccttac aaaaacagtt
     6901 tattgaagac atcaaagaac acagccgccc gattacttta gaacaacaat cggaagtggt
     6961 aggcaaatgt ttaaaagaaa ttccggtgat tatcaaaaaa ctaagcgaat ccagtttgcc
     7021 gattgaggaa attcgtgcac gttactacgc ctttttccaa gatctgttta gcaaagtgga
     7081 ttttaaaacc attttcagag ccgatattca gcaaattatt caatttgcca agcagaatca
     7141 aattaagctt gcggttgcct cttcttccgc cctttcgcat attgaaaata ttctcacggt
     7201 ttgcggcatt aaagacgaat ttgatttaat cgtcagcggt gaacagtttg aacacagcaa
     7261 gccagaccca accatttacc gctacacctg cgaaaaatta ggcgttgagc cacaaaatgc
     7321 agtggcgatt gaagactcct actacggaat gctcgccgcc aaaaccgcag gcttaacggt
     7381 gattggttac gaagaaaaac gaatactgat tgaccaaagt ttagcggatt atatgggcaa
     7441 agatatgaaa gagattttga agattcttca aaaatgtaat actccaagtt aataaacggt
     7501 cgatttttat agaaattttg tcaatgtttc tacttcaagt acttg
//
LOCUS       DANLYI010000115         7513 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.115, whole genome shotgun sequence.
ACCESSION   DANLYI010000115 DANLYI010000000
VERSION     DANLYI010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 7513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7513
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.115"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            20..1483
                     /gene="ubiD"
                     /locus_tag="PXD81_001693"
     CDS             20..1483
                     /gene="ubiD"
                     /locus_tag="PXD81_001693"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005617934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HDL5537700.1"
                     /translation="MKYKNLREFLDFLESQGELVRIQQEIDPYLEMTEIADRTLRKGG
                     PALLFENPKGYQMSVLCNLFGTPKRVALGMGQEDTKALRELGKLLAFLKEPEPPKGFK
                     DLIGQLPQWKQILNMPPKVLSKADCQQVVISGDEVDLYKLPIMHCWPGDVAPLVTWGL
                     TITQGPYKKRQNLGIYRQQLIGKNKLIMRWLSHRGGALDFQEWKAENPGKPFLVSVAI
                     GADPATILAAVTPVPDTLSEYAFAGLLRGHKTEVVKSISNELEVPASSEIVLEGYIDL
                     EETALEGPYGDHTGYYNEQEYFPVFTVTHITMRKDAIYHSTYTGRPPDEPAVLGEALN
                     EVFIPILQKQFPEIVDFYLPPEGCSYRLAVVTMKKQYAGHAKRVMMGVWSFLRQFMYT
                     KFVIVCDDDVNARDWKDVIWAITTRCDPARDTTLIEHTPIDYLDFASPIAGLGSKMGI
                     DATNKWAGETTREWGTPIEKDDNVVKRIDEIWDSLGI"
     gene            complement(1568..2392)
                     /locus_tag="PXD81_001694"
     CDS             complement(1568..2392)
                     /locus_tag="PXD81_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL5537701.1"
                     /translation="MDSLTHLFTQFQFRTDLFHLGQLCQIGSFNEENKGYLHFVRQGR
                     FILNQAAERPITIERPAIIFSPTNILHSLHPLDDSGLDIFCINFDFGKGIRNPLTHTL
                     HNIVILELAQYPELGVLADQIFSEVNQKGCGYQAMIHHLCAYFTLKVARQCLEKGYIQ
                     TGLLKGLADKQLGKVLLAIHNAPERDWNVEELANVALMSRSRFAAYFKQTMGVSPMDY
                     LTNWRLAVAQHLLQKGIPVALVAEQVGYSHNAALSRVFMRELGLSPTEWLAQNKES"
     gene            2519..3364
                     /locus_tag="PXD81_001695"
     CDS             2519..3364
                     /locus_tag="PXD81_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HDL5537702.1"
                     /translation="MMSQEQFEEINQERRQALNHGSRIAMAASVLGALGIASTANAKQ
                     ASAEKSPYAEPEQYGLKRHGMTIDPKRVALVVVDPQIDFLGPKGVMWGVLGDSIKENN
                     TVANIESLFKAAKAVDMPTFVSPHYYYPTDHKWEFGSPGEHFMHDSGMFARKGQYTME
                     GFENSGADFMPEYKPYIFDGKTVITSPHKIFGPETNDLVLQLRKRKIDQVILAGMAAN
                     LCIDSHLRELIEQGFEVTVVKDATAGPRIPEGDGYLAALTNYRIIANDLWTTEEAVKK
                     MTAKA"
     gene            3821..6763
                     /gene="rne"
                     /locus_tag="PXD81_001696"
     CDS             3821..6763
                     /gene="rne"
                     /locus_tag="PXD81_001696"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HDL5537703.1"
                     /translation="MKRMLINATQKEELRVALVDGQRLFDLDIESPGHEQKKSNIYKG
                     KITRVEPSLEAAFVDYGAERHGFLPLKEISREYFPSDYVFNGRPNIKDIIQEGQEVIV
                     QVSKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDERLELKEALDCLDV
                     PEDVGLIVRTAGVGKSPEELQWDLKVLLHHWEAIKKAAESRPAPFLIHQESDVIVRAI
                     RDYLRRDIGEILIDNKKVFEKAKNHIRLVRPDFINRVKLYEGEVPLFSHYQIESQIES
                     AFQREVRLPSGGSIVIDVTEALTAIDINSSRSTRGGDIEETALNTNLEAADEIARQLR
                     LRDLGGLIVIDFIDMTPVRHQREVENRIREATRQDRARIQFARISRFGLLEMSRQRLS
                     PSLSEASSHICPRCQGTGKVRDNESIALSILRLLEEEAIKENTAQVHTIVPVQIASYL
                     LNEKRKAISSIEKRHDVQIVVVPSESMETPHFSVYRLRENEITPTLSYDLTKHYEARH
                     EEEHPHHHHQEDVLVTRNEPVITVESVLQSSELNLQPSPTPTEPPKPSLLSRLIATIK
                     GWFSEEKVEEKPKPKARNQRERRQRRERPNRNAKAENQERKTPQAQTTPAEEKPMRSE
                     KKARRAVVEEVSGVTEQESRSNSREVQTERRQRRDLRKKVRIETQEVEVENLTPAVEE
                     TVAKQVENVQKNQQIQPLATQEVPVETAPEVETTNEGRENNRQRRLPRHLRVGNQRRR
                     ENQRNQVISPMPLTSAVASPELASGKVWVQVTSTVKVEEKASFLSVDQMLEQQENQLV
                     ETEKKSEEKKLHTVSPLGGFVAQATNRDLVKEKQEQLSKPKEEKVASEDVKPVEVKEH
                     IAPFGSVVAQHSERDLVKEKNERVSKFQSQATVAPAIVEEVTNPIEVPKSTYESDYRF
                     NGRLGTFSRVEHTQAAMTIASAPSDKLEVVEISQWQNSKYYFYGKGFGGYTSAVSQVQ
                     SAPRSTNG"
     gene            6909..7469
                     /locus_tag="PXD81_001697"
     CDS             6909..7469
                     /locus_tag="PXD81_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5537704.1"
                     /translation="MKKSFLSLLVLGTTMALAACDQAKEAAQSTTDKAVEVKEAVAEK
                     AAEVKSATEAKVEELKAAANDAAQATADKTEEAKKAVAEKATELKTTTEAKVEELKAA
                     ANDAAQATTDKTEEAKKAVAEKAAELKATTEAKVEELKAAASDATQATADKAEEAKKA
                     VAEKAAEVKVETETKVEEMKKAIESK"
BASE COUNT         2432 a         1588 c         1649 g         1844 t
ORIGIN      
        1 atcgaaaaat atgaccgcta tgaagtataa aaatctccgt gaatttctcg atttccttga
       61 gtctcagggt gaattagtcc gtattcagca agaaatcgat ccttatttag aaatgacaga
      121 aatcgccgac cgcaccttac gcaaaggcgg gccggcatta ctgtttgaaa acccgaaagg
      181 ctatcaaatg tcggtgttgt gtaatttatt cggcacgccc aaacgggtag ctctgggaat
      241 ggggcaagaa gataccaaag ctctgcgtga gctgggcaag ttacttgcct ttttaaaaga
      301 gcctgaacca ccgaaagggt ttaaagattt gatcgggcaa ctgccacaat ggaagcaaat
      361 tttaaatatg ccgcctaagg tactgagcaa agccgattgt cagcaagtgg tgatttcggg
      421 tgatgaggtt gatctctaca aattaccgat tatgcactgt tggccgggcg atgtcgctcc
      481 actggtcact tgggggctga cgattacgca agggccttac aaaaaacgcc agaatttagg
      541 catttatcgt cagcaactta tcggcaaaaa caaactgatt atgcgttggc tttcccaccg
      601 tggcggtgcg ttagacttcc aagagtggaa agccgaaaac ccgggtaaac cgtttctggt
      661 gtcggttgcg attggtgcag atccagccac tattttagcg gcagtcaccc ctgtgccgga
      721 tacgctttcg gaatatgcgt tcgcagggct gttgcgaggg cataaaaccg aagtggtcaa
      781 atctatttcc aatgagttag aagtgccggc aagtagcgaa attgtgctgg agggctatat
      841 cgatctggaa gaaaccgcat tggaaggccc ttatggcgac cacaccggct actacaacga
      901 gcaagagtat tttccggtat ttaccgttac ccatattacg atgcgaaaag atgcgattta
      961 tcattctacc tacactggca gaccacctga tgaaccggca gtattaggtg aagcattgaa
     1021 tgaagtcttt attccgattt tgcaaaaaca gtttccggaa attgtcgatt tctacttgcc
     1081 acctgaaggc tgttcttacc gtttagcggt agtgacgatg aaaaaacaat atgccggaca
     1141 cgctaaacgg gtgatgatgg gagtttggtc gttcctgcgt caatttatgt acaccaagtt
     1201 tgtgattgtg tgcgatgacg atgtgaacgc tcgagattgg aaagatgtaa tttgggcgat
     1261 caccacccgt tgtgacccgg ctcgagatac gacactgatt gaacacacgc cgatagatta
     1321 tctcgatttt gcctcgccaa ttgcagggtt aggctcaaaa atggggattg atgccaccaa
     1381 taaatgggcg ggtgaaacca cccgagaatg gggaacaccc atcgaaaaag atgacaatgt
     1441 ggtgaaacgc attgatgaga tttgggattc tcttggcatt taaggcaaca tttaaccgct
     1501 tgtgcaacat aacaagcggt taaatttttt gacattttta ccattcggct atttgtttct
     1561 catcaggtta cgactcttta ttttgagcca gccactcggt aggcgaaagc cctaattccc
     1621 gcataaagac acggctgaga gcagcattat ggctataacc gacttgctct gccaccaatg
     1681 ctactggtat ccctttttgt aataaatgct gggcaaccgc tagtcgccag ttagtgagat
     1741 aatccatcgg tgaaaccccc atcgtttgtt taaaataagc agcaaagcgt gagcgagaca
     1801 ttaatgccac atttgccaac tcctctacat tccaatctcg ctctggtgca ttgtgaatag
     1861 ctaacagcac tttccccagt tgcttatctg ccaagccttt taacaaacct gtttgaatat
     1921 accctttttc caaacattgg cgggcaactt tcaaggtaaa ataggcacat aaatgatgaa
     1981 tcattgcctg ataaccacac cctttctgat tcacttcact aaaaatttga tcggctaata
     2041 cccccaattc cggatactgt gccaactcta agatcacaat attgtgcaag gtatgagtta
     2101 atgggttgcg aatccctttg ccaaaatcaa aattaataca aaatatatct aacccactat
     2161 catctaacgg gtgcaaacta tgcaaaatat tggttggtga aaaaataatc gctgggcgtt
     2221 caatcgtaat cgggcgttcg gcagcctgat ttaaaataaa acgtccctga cgaacgaaat
     2281 gtaaatagcc tttattctct tcattaaaac tcccgatttg gcataactga cctagatgaa
     2341 ataaatccgt gcgaaactga aattgggtaa aaagatgagt caaactatcc acattaagct
     2401 ccttgggtaa agaattgaga cgaacggtta aaacaggaga aatgcgatca ctataatagc
     2461 aacccgttcg gtaacgcaag ttatcattac aatttgtata tctattaatg aggataaaat
     2521 gatgtcacag gaacaatttg aagaaatcaa ccaagaacgc cgtcaagccc ttaaccacgg
     2581 ctctcgtatt gcaatggcag ccagtgtatt aggtgcatta ggtattgcat caacagcaaa
     2641 tgcaaaacaa gccagtgcag aaaaatcacc ttatgccgag ccggaacaat acggtttaaa
     2701 acgccatgga atgaccattg atccaaaacg tgtggcatta gtggtagttg atccacaaat
     2761 tgacttccta ggcccgaaag gcgtgatgtg gggcgtgtta ggtgactcaa ttaaagaaaa
     2821 caacactgta gctaacattg aaagcttatt taaagcagca aaagcagtcg atatgccaac
     2881 ctttgtttcg ccacactact attacccgac tgatcacaaa tgggaatttg gttcaccggg
     2941 tgaacacttt atgcacgaca gcggaatgtt tgcgagaaaa ggtcaataca ccatggaagg
     3001 ctttgaaaat tcaggtgcag actttatgcc tgagtacaaa ccatacattt ttgatggcaa
     3061 aacagtgatt acctctccac acaaaatctt tggaccggaa accaatgact tagtgttgca
     3121 attacgcaaa cgtaaaatcg accaagtaat tttagccggt atggctgcca acttatgtat
     3181 tgattcacac ttgcgtgaat taatcgaaca aggttttgaa gtaacagtgg tgaaagatgc
     3241 aaccgcaggg cctcgtattc ctgaaggtga cggctacctt gcagcattaa ccaactaccg
     3301 tattatcgca aatgacctct ggaccactga agaagcagtg aaaaaaatga cggcaaaagc
     3361 ataatattcg ctttacaagc ggttaaattt agctcttttt ttgcaaattt aaccgcttgg
     3421 atttcacgct tatctccagc actcacacta aatctcatta tttttcctta tcaccccatt
     3481 cattttcatt atattcatct ccacaacaaa taacagctac catacaacaa ttaccttgcc
     3541 tttttcaggg ttaaaatgcg ataatgactc gttatttttg gcttgaaaaa gcaatagaaa
     3601 attgcaaaaa ataaagcaaa aataaccgct tgtaatagag ataaacaagc cttaagtgat
     3661 tgattttgca gtttctttaa gacaagacaa agtaaacgta agacaaccat caatgcacta
     3721 tcgggaatga agacagtcgt gagatagaca ttaaatccat tcccttatta atgggtcaaa
     3781 atgaccgtgt aagacaaaaa caaaataatg agaaaacaca atgaaaagaa tgttaatcaa
     3841 tgcaactcaa aaagaagagt tgcgtgttgc cctcgttgat gggcaacgct tatttgactt
     3901 ggacattgaa agcccgggtc acgaacagaa aaaatccaat atctacaagg gtaaaattac
     3961 ccgtgtcgaa ccaagccttg aagctgcatt cgtagattac ggtgcagaac gccacggctt
     4021 cctaccttta aaagaaattt cccgagaata cttcccgtca gactatgtgt ttaacggtcg
     4081 cccgaatatc aaagatatta ttcaagaagg gcaagaagtg attgttcaag tcagcaaaga
     4141 agagcgtggt aacaaaggag cagccttaac cacctttatt tcgcttgcgg gcagttactt
     4201 agtcttaatg ccgaacaacc ctcgtgccgg tggtatttcc cgccgtattg aaggtgatga
     4261 gcgtttagaa ctcaaagagg ctttagactg cttagatgta ccggaagacg tgggcttaat
     4321 cgttcgtacc gccggtgtcg gcaaatcgcc ggaagagtta caatgggact taaaagtctt
     4381 gttacaccac tgggaagcga ttaaaaaagc ggcggaatct cgcccagctc cgttcttaat
     4441 tcaccaagaa agtgacgtga ttgtgcgtgc aattcgtgac tatctacgcc gtgatattgg
     4501 cgaaatctta atcgacaaca aaaaagtgtt cgagaaagcc aaaaaccaca ttcgccttgt
     4561 tcgcccggat ttcatcaacc gtgtgaaatt atacgaaggc gaagtaccac tattcagcca
     4621 ctatcaaatt gaatcgcaaa tcgaatcggc attccaacgt gaagtgcgtt taccttccgg
     4681 tggctctatc gttattgatg taacagaagc cttaacagca attgatatta actcctcacg
     4741 ttcaacccgt ggcggcgata ttgaagaaac cgctcttaac accaacttag aagcggcaga
     4801 tgaaattgcc cgtcagttac gtttgcgtga cttaggtggt ttgattgtga tcgactttat
     4861 cgatatgaca ccggttcgcc accaacgtga agtagaaaac cgtattcgtg aggcgacacg
     4921 ccaagaccgt gcgagaatcc aatttgcccg tatttcccgt ttcggtttgt tagaaatgag
     4981 ccgtcagcgt ttaagcccat cattaagtga ggcttcatcg catatctgcc ctcgctgtca
     5041 gggaactggt aaagtacgtg ataatgaatc aatcgcactg tcaatcttac gtttattaga
     5101 agaagaggca atcaaagaaa acactgcaca ggttcacacc attgtgccgg tgcaaatcgc
     5161 cagctactta ctcaacgaaa aacgtaaagc gatttccagc attgaaaaac gccacgatgt
     5221 acaaattgtt gtagtaccgt ctgaaagtat ggaaacgccg catttcagcg tatatcgcct
     5281 gcgtgaaaat gaaattactc caacgttaag ctacgattta accaagcatt acgaagctcg
     5341 tcatgaagaa gaacacccac atcatcacca tcaagaagat gtgctggtta cccgtaacga
     5401 gccggtgatt acggtagagt cagtgttaca aagctcggag ctgaatctcc aaccttcgcc
     5461 aacaccaacc gaaccaccaa aaccatcgct gttaagccgc ttaattgcga ccattaaagg
     5521 ctggtttagt gaagaaaaag tggaagaaaa accgaagccg aaagcacgca atcagcgtga
     5581 gcgtcgccaa cgccgtgaac gcccgaaccg taatgctaaa gcggaaaatc aagagcgtaa
     5641 aactccgcaa gctcaaacta ctccagcgga agaaaaaccg atgcgtagcg agaaaaaagc
     5701 acgccgagcg gtagtggaag aagtttccgg tgtaaccgag caagagagtc gtagcaactc
     5761 ccgtgaggtt caaactgaac gtcgccaacg ccgtgatcta cgtaaaaaag tgcgtatcga
     5821 aacacaagaa gtagaagtgg aaaatctaac acctgctgtt gaggaaacgg tggcaaaaca
     5881 agttgaaaat gtgcaaaaaa atcaacaaat tcaaccgctt gcaacccaag aagtacctgt
     5941 tgaaacagct cctgaggtag aaacaacgaa cgaaggacgt gaaaataacc gccaacgccg
     6001 tttacctcgc catttgcgtg tgggcaatca gcgtcgtcgt gaaaatcaac gcaatcaggt
     6061 tatttctcct atgcctctaa cttcagctgt tgcttcgcct gagcttgcga gcggtaaagt
     6121 ttgggttcag gttacctcta ctgtcaaagt agaggaaaaa gcatcattct tatctgttga
     6181 tcaaatgctt gaacagcaag aaaatcagct tgttgagaca gagaaaaaat cagaagagaa
     6241 gaagcttcat acagttagtc cgctaggtgg ctttgtagca caagcaacta accgtgattt
     6301 ggttaaggag aagcaagaac agctttccaa accgaaagaa gaaaaagttg cttctgaaga
     6361 cgtaaaacca gtagaggtaa aagagcatat tgctccattt ggtagtgttg ttgctcagca
     6421 ttctgaacgt gatttagtta aagagaaaaa tgagcgagtc agtaaattcc aatcgcaagc
     6481 aacggttgca ccagcgattg tggaagaggt tactaacccg attgaagtgc caaaatcaac
     6541 ttacgaaagc gattatcgat ttaacggcag attaggtaca ttctctcgtg ttgagcatac
     6601 ccaagccgct atgaccattg cttctgcacc aagtgacaag cttgaagtag tcgaaattag
     6661 ccaatggcaa aactctaaat actatttcta tggcaaaggg tttggcggat ataccagcgc
     6721 agtgagccaa gtgcagtcag cgcctcgttc aaccaatggt taagtacaat taattggttt
     6781 aaatgcctat tagttataac taataggcat ttttttatca aagtatgaac tttctatcaa
     6841 tttttctgcc taagtaaacg cactttatta caaagtgcgt tatttttagt ttatttaagg
     6901 aactcggtat gaaaaaatca tttttatcat tattagtgtt aggtacaact atggcacttg
     6961 ctgcttgtga tcaggcaaaa gaagccgcgc aatcaactac agacaaagcg gttgaagtaa
     7021 aagaagctgt agctgaaaaa gctgcagaag ttaaatcggc tactgaagcg aaggttgaag
     7081 agttgaaagc ggcagcaaat gatgcagctc aagcaacagc agataaaaca gaagaggcaa
     7141 aaaaggcagt ggcagagaaa gccactgagt taaaaaccac aactgaagct aaagtggaag
     7201 aactaaaagc agcagcaaat gatgcagctc aagcaacaac agataaaaca gaagaggcaa
     7261 aaaaggcagt agcagagaaa gctgctgagt taaaagccac aactgaagct aaagtggaag
     7321 aactaaaagc agcagcaagt gatgcgactc aagcaacagc agataaagca gaagaagcta
     7381 aaaaggcagt agctgagaag gcagcagaag tgaaagttga gacagaaaca aaagttgaag
     7441 aaatgaaaaa agcgattgaa agcaaatagt gctttttagt ttctctgtag ctatcgaaac
     7501 acgctaaaaa taa
//
LOCUS       DANLYI010000116         7495 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.116, whole genome shotgun sequence.
ACCESSION   DANLYI010000116 DANLYI010000000
VERSION     DANLYI010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 7495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7495
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.116"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            162..830
                     /locus_tag="PXD81_001698"
     CDS             162..830
                     /locus_tag="PXD81_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL5537705.1"
                     /translation="MAHNGSGKSTLMRLLSGDITPTSGSILVDGLPLHQLSTKEAAKH
                     IAYLPQRLPEAADFLVQELVALGRFPYQKWLQKPTEFDVQIVEQAMESTSVTQFAKQP
                     VNSLSGGEKQRVWLAMCLAQQTPYLLLDEPLAALDIVYQVEVMQLIRKLVNEQGLTVI
                     IIIHDINLASQFCDEIIALKQGELCIQGSVDEIMQEQKLLEIFGLKLHLFNHPAGLHK
                     VAVL"
     gene            827..1708
                     /locus_tag="PXD81_001699"
     CDS             827..1708
                     /locus_tag="PXD81_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL5537706.1"
                     /translation="MMFRQYKRFIFLFILQISTAFAQPNIFTPDWSIASALTEMGNPP
                     IAMGDKRIYPLWSHRPIQPNSVLDIGARYQPNRELLAQLPVELVLSNFFYAHLASVYP
                     KEIPQIDVLFDGGNTEPQQHWHTYQKATRMIGTEIKNPNAAEAYLTKTEQTLAQWGKE
                     ILNSNPDIDYYSVVQFGSPRELRIYASNSMFRVAFDLMGLKQADLGVGDRWGNKLIPV
                     SDLAKLPERTCLIVISPFSKMTQADLENSYIWQRLGFGKNRCMKVLPPVWLFGGADSI
                     SHFAEFLHNAMTQGEKE"
     gene            1708..2523
                     /locus_tag="PXD81_001700"
     CDS             1708..2523
                     /locus_tag="PXD81_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HDL5537707.1"
                     /translation="MKSSFLLIGCILLSLVTAVIRLNMVWEWEWQLLLINVDLLPINQ
                     AAVKAALLPEMTTSFLAGGLLAIASSGLQHIVRNNLASDSTLAVSSGAQLALMIAVIF
                     FPAAELFSSFWIALLGSLAAIVLVLFIAKASKLNPLTLILGGLIVNILFGAIASLLTL
                     FYFDFLFGVMVWGSGSLLQDGWATSITLAITVVVAFFIFVLLSRPLTILSLDDEQARR
                     LGAPVNLLRYLVIFVCAAITALVVSKIGVIGFIGFAVQVWLILPKYDIYYFAS"
     gene            2520..3656
                     /locus_tag="PXD81_001701"
     CDS             2520..3656
                     /locus_tag="PXD81_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HDL5537708.1"
                     /translation="MIAFLAGGFLLFFTNNLIGIVSRIYDTLLPVGAFTATLGAPLLI
                     FLVLRQRKAQFEASNTAYFPVKRLNSWKLPILLLCAGVILFCLLQIIAPTMNGWAISI
                     DENLMSSYRLPRSLSAIATGAMLAVSGSLLQILTRNPMASPEVLGVSSGATIAAVLSF
                     LLFPTLSSISFLLFGVLGSLTVLALIMWLSRKLQPSALMITGVAIGALVSGLMAIIQL
                     SGSPRLTAVISWLSGSTYYANPNTVWILLSVAILLISATLLFIKPLRLISLGETVSYH
                     LGLEVKKVQMIVLLLIALMSAASTLAVGPMSFIGLMTPHLAKNLGAITPEKQIPISAL
                     LGAMLMLIADWLGRYLIFPYEIGAGIIASILGGIYFLLLLKQHK"
     gene            3684..4082
                     /locus_tag="PXD81_001702"
     CDS             3684..4082
                     /locus_tag="PXD81_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HDL5537709.1"
                     /translation="MNTLSKAIKLALYSSGIGFISTAYAETVTHLDTVEVSAETPNAS
                     SEETGRYAQKKAKGSNGMALSAKDTPQSVTAVTHQQIRDQNLNTIAKALEATHGVSVS
                     LLDRGRYSFSARGFGIDKVKVDGMDLKVSK"
     gene            4117..5820
                     /locus_tag="PXD81_001703"
     CDS             4117..5820
                     /locus_tag="PXD81_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HDL5537710.1"
                     /translation="MLYDRVEVVRGATGLTTGAGDPSASVNLVRKRANSISRYTILEA
                     GIGRYADFNTMIDHSQALNNSGSVRGRFIAEHKNGNTFIKNEKERYTTLYGNIEADLT
                     DTTKLGLGVSYQKEKRDAALWGGLPAFYSDGGETNWSRSKNASPEWAYWDNESVNYFA
                     DITQKLGEKWELALKGNYRVGKHNSELFYFSGSSLNRQDGLGWSPWPGKFKNDNTQSN
                     VQLDLTGSFSAWGLEHDAAIGIQYNRAHRNSYAADNSTFDGAFDFNNWNGIYARPNWG
                     SMSEKYYQNLKEFGVYVSTRLHLTDRLSMLLGSRISSYRTEGTFYGSTQNFHANDVWT
                     PYAGLTFDINPQNTVYVSYTDIFKPQSQVDINNKLLSPVTGETYEAGWKGSYFNDNLQ
                     TQISVFETRQDNLAQSAGINPNTLLAYYTASKGAKVRGFEVEASGNVTEKLKLSAGYT
                     QWKGDDASGNPINTTQPRKQFKLFATYDMNRLVQGLTVGAGVNWQSRIYTKHATYGEY
                     GQKAYTLVNLMARYQFNDNFSAQLNIDNLFDKKYRNALSFSQYSYGEPFYTRLNLRYE
                     F"
     gene            5932..6576
                     /gene="adk"
                     /locus_tag="PXD81_001704"
     CDS             5932..6576
                     /gene="adk"
                     /locus_tag="PXD81_001704"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005721186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HDL5537711.1"
                     /translation="MKVILLGAPGAGKGTQAQFIMNKFGIPQISTGDMFRAAIKESTE
                     LGKQAKALMDEGKLVPDELTVALVKDRIAQPDCANGFLLDGFPRTIPQADALKESGVK
                     IDLVLEFDVADEVIVERMSGRRVHQPSGRTYHVVYNPPKVEGKDDVTGEDLIIRQDDK
                     PETVLERLAIYHKQTKPLIAYYTAEAEAGNTRYERLDGTKPVEEVSAELAKILS"
     gene            complement(6638..7453)
                     /locus_tag="PXD81_001705"
     CDS             complement(6638..7453)
                     /locus_tag="PXD81_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HDL5537712.1"
                     /translation="MSYKVVFSLLFSLFLSTANANWTKINDVNYTWGPFNIYHITLFT
                     ESGTYVEGTRPLMLTLQYKKPWEGRDFAVSLARTWNNLGIKLPEKELDEVITHLRKTF
                     PDIKPEDTLHYIALEDRGYFILNDKVVSDVFNKAFNDAIVAIWLDPKMDISHQLLDPS
                     YKPRAEAESIKPQVEERVAENLQEPIVNTEVSKELKADSQAVEQAPNKIDIPVADKTQ
                     ASVVNEATQPVENKDKKEPEKLEEKPKEMKIENPEVFISPIYDDILMLTKYCV"
BASE COUNT         2194 a         1478 c         1480 g         2343 t
ORIGIN      
        1 ctactcattt tcattttgat tgatttggtg gggtaatccc acctttttta tgctcttaaa
       61 acacaacact atgattcaaa taaattccat tagctactct gttgaacaaa accgtaaact
      121 gctaagcaat atttccctca atttaagcaa ggggcgttta tatggcgcat aacggctcgg
      181 gtaaatcaac gttaatgcgt ttactaagcg gagatataac gcctaccagc ggttcaattt
      241 tagtcgatgg attaccactt catcagcttt ctaccaaaga agcggcaaaa catattgcct
      301 atttaccaca acgtttacct gaagcggcgg atttcttagt gcaggaactg gttgctttag
      361 ggcgatttcc atatcaaaaa tggctacaaa aaccaacaga atttgatgtt caaattgttg
      421 agcaagcaat ggaaagcacc tccgttactc agtttgctaa acagcctgta aatagtctat
      481 caggcggaga aaaacaacga gtttggcttg ctatgtgctt agcccaacaa acaccctatt
      541 tactgctaga tgagccattg gctgcccttg atattgttta tcaagttgaa gtaatgcagc
      601 ttattcgcaa gctagtgaat gaacaaggtc taactgtcat tatcattatt cacgatatta
      661 atcttgcctc ccaattttgt gatgaaatta ttgcgttaaa gcaaggcgaa ctctgtattc
      721 aaggttctgt tgatgaaatt atgcaagaac agaaattact tgagatcttc gggctaaaac
      781 tgcacttgtt taaccaccct gcaggcttac ataaagtggc ggtattatga tgtttaggca
      841 atacaagcgg ttcatttttc tctttatttt gcaaatttct accgcatttg ctcaacctaa
      901 tatttttaca ccagattggt ctattgcctc tgcccttact gaaatgggaa atccgcccat
      961 tgcgatgggg gataagcgaa tttatccgct atggtcgcac cgccctattc agcctaatag
     1021 cgtgttagat attggagcgc gttatcagcc taatcgcgaa ttattagcac agcttcctgt
     1081 tgaattggtg ctatcaaact ttttctacgc tcacttggct tctgtttacc cgaaagaaat
     1141 tccacaaata gacgtgctat ttgatggtgg caatactgaa cctcagcaac attggcatac
     1201 ttaccaaaaa gccactcgta tgattggaac agagattaaa aaccctaatg cggcagaagc
     1261 atatttaacg aaaactgaac aaacgttggc tcaatggggg aaagaaatcc ttaacagtaa
     1321 tccagatatt gattactact ctgttgttca atttggctct ccgagagagt tacgcatata
     1381 tgcgtctaat tcaatgttcc gtgtagcctt tgatttgatg ggcttaaaac aagctgattt
     1441 aggtgtggga gatcgctggg gaaataaact tattcctgtg agtgatttag ctaaattacc
     1501 cgaacgcacc tgtttaattg tcatctcacc tttttctaaa atgacgcaag cagatttaga
     1561 aaatagctac atttggcaac gtttaggatt tggtaaaaac cgttgtatga aagtattacc
     1621 gcctgtttgg ctctttggtg gggctgattc catctcgcac tttgctgagt ttttacacaa
     1681 tgcaatgaca caaggagaaa aagagtaatg aaatcatcat tcttattgat tggctgtatt
     1741 cttttatcct tagtaacagc tgttatccgt ttaaatatgg tctgggagtg ggaatggcag
     1801 ttactactca taaatgttga tttattgcca attaatcaag ctgcggtaaa agcagctctt
     1861 ttacctgaaa tgacaacgtc atttttagca ggcggtttat tggctatagc cagcagtggc
     1921 ttacaacata ttgtcagaaa taatttagcg tcagatagca cacttgccgt cagtagcggg
     1981 gctcaacttg cattgatgat tgcggttatc tttttcccag ccgccgagct tttttctagt
     2041 ttctggattg cgttacttgg ctctttagct gctatcgtac tggttttatt tattgctaaa
     2101 gcctctaagc tcaatcccct tacccttatt ttagggggat tgattgttaa tattttgttt
     2161 ggagctattg cctctttact gacccttttc tactttgatt ttttatttgg cgtgatggtt
     2221 tggggaagtg gctcactttt acaagatggt tgggcaactt ctattacctt agcgataacg
     2281 gtagtagttg cattttttat ctttgtctta ctctctcgtc cactcacaat tttaagttta
     2341 gatgatgaac aagctcgccg cttaggcgcg cctgttaatt tactacgcta cttagttatt
     2401 tttgtttgtg ccgccattac agcattggtt gtaagcaaaa taggtgtgat tggctttatt
     2461 ggatttgcag tgcaagtttg gctcattttg ccaaaatacg acatttatta cttcgcctct
     2521 tgattgcttt tctagcgggc ggatttttac tcttttttac caataatctc atcggcattg
     2581 ttagcagaat ttacgacact ctactacctg ttggtgcatt taccgcaaca ctaggcgcac
     2641 cattattaat tttcttggta ttacgccaac gtaaagcaca atttgaagca agcaatacag
     2701 catacttccc cgttaaacgc ctcaattcgt ggaaactgcc aatattgttg ttatgcgctg
     2761 gagttatcct attttgtcta ttacaaatca ttgctccaac aatgaatggc tgggctatta
     2821 gcattgatga aaacctgatg agttcctacc gcttgcctcg tagtctaagt gcgatagcaa
     2881 caggggcaat gcttgcggta agtggtagcc tattacaaat cttaacgaga aacccaatgg
     2941 caagccctga agtattaggt gtgagttcag gggcaactat tgctgcagta ctgtcattct
     3001 tacttttccc aacattatct agcattagct tcttgttatt tggtgtatta gggagtttga
     3061 ctgttctcgc tcttattatg tggctctctc gtaaattaca accatcagcg ttaatgatta
     3121 caggtgttgc tattggagct ttagtcagtg gcttaatggc tattattcaa ctaagcggca
     3181 gccctcgttt aacggcagtt attagttggc tgtctggctc aacctattac gctaatccga
     3241 atacagtttg gattttatta tctgttgcaa tcctacttat cagtgccact ttactgttta
     3301 tcaaaccttt acgtttgatc tcacttggcg agaccgtttc ttaccattta gggttagagg
     3361 ttaaaaaagt acaaatgatt gtattacttt taattgccct aatgagtgcc gcctctacac
     3421 ttgcggtagg gccaatgagc ttcattgggc taatgacccc tcatttagct aaaaatttag
     3481 gtgcaattac accggagaaa cagatcccta tatcggcatt attaggggca atgctaatgc
     3541 tcattgccga ttggctcggg cgttatctaa ttttccctta tgagattggg gcaggcatta
     3601 ttgcctctat tctcgggggt atctatttct tactgttact caaacaacat aaataatttt
     3661 ttattcatta cttaggagaa attatgaaca cgctatctaa agcgataaaa cttgccttat
     3721 acagttctgg tatagggttt atctcaacag cttacgctga aacagttact catcttgata
     3781 ccgtagaagt aagtgccgaa acgccaaatg cctcatcgga agaaactgga cgttatgcac
     3841 agaaaaaagc caaaggctct aatggaatgg cattgagtgc gaaagacacg ccacaaagcg
     3901 taacggctgt aacgcatcaa caaatcagag atcaaaatct taatacgatt gccaaagcac
     3961 ttgaagcaac tcacggtgtt tctgtttctt tacttgacag aggacgctat tctttcagtg
     4021 ctcgtggttt tgggattgat aaagtcaagg tagatggtat ggatctcaaa gtaagtaaat
     4081 aaccaatgga caacgggcga aaatacaggc aacagcgtgt tatacgaccg agtagaagtt
     4141 gtgcgtggtg ctacaggctt aactactggg gcaggcgatc cttctgcaag tgttaatctt
     4201 gttcgtaaac gagccaatag catttcccgt tatactattt tagaagctgg aatcgggcgt
     4261 tatgctgatt tcaatacaat gattgaccat agccaagcat tgaataattc aggatcagta
     4321 agaggccgct ttattgcaga gcataaaaac ggtaatacgt tcattaaaaa tgaaaaagaa
     4381 cgttacacca cgctttatgg caatattgaa gcagacttaa ctgacactac caaacttggg
     4441 ttaggtgtaa gctatcaaaa agaaaaacgt gatgcagcac tttggggtgg tttacccgca
     4501 ttctatagcg acggtggtga aactaactgg agcagaagca aaaatgccag tccggaatgg
     4561 gcttactggg ataatgaatc cgtaaactat tttgccgata ttacacaaaa actaggcgaa
     4621 aaatgggaac ttgcattaaa aggcaattac cgtgtaggca aacataattc agaacttttc
     4681 tatttcagcg gctcatcact taatcgccaa gatggattag gttggtcgcc ttggccgggt
     4741 aaatttaaaa atgataatac acaatctaat gtgcagcttg atcttaccgg ttcattctct
     4801 gcttggggat tagaacacga tgcggcaatc ggtattcaat acaatcgagc acatcgtaat
     4861 tcctacgctg ccgataatag cacctttgac ggagctttcg actttaataa ttggaatggc
     4921 atttatgcta gaccaaactg gggaagtatg tctgaaaaat actaccaaaa cttaaaagag
     4981 ttcggggtgt atgtttctac ccgtttacat cttacagata gactttctat gcttctaggt
     5041 tctcgtattt catcgtaccg cacagaaggc actttctatg gttcaacaca aaacttccac
     5101 gctaacgatg tttggacacc ttatgcagga ttaacctttg atattaatcc acaaaatacc
     5161 gtttatgtaa gttataccga tattttcaaa ccacaaagtc aagtggacat caataacaaa
     5221 ctactctctc ctgttacggg cgaaacctac gaagcaggtt ggaaagggag ttatttcaat
     5281 gacaacttgc aaactcaaat tagcgtattt gaaacaagac aagataatct agctcaatct
     5341 gccggtatta accctaatac attattagcc tactacacag cctctaaagg tgcgaaagtt
     5401 cgaggctttg aagtagaagc atctggaaat gtaactgaaa aattaaaact cagtgcagga
     5461 tatactcagt ggaaaggcga tgatgcttca ggtaacccaa ttaacacaac tcaaccacgc
     5521 aaacaattta aactctttgc aacttacgat atgaaccgtt tagtgcaagg tttaacggta
     5581 ggagcaggtg taaactggca aagcagaatt tataccaaac acgctactta tggcgaatat
     5641 ggtcaaaaag cttacacttt agtgaatttg atggctcgtt atcaatttaa tgacaacttc
     5701 tcagcacagc ttaacattga taacttattc gataaaaaat atcgtaacgc cctcagcttt
     5761 agccaatata gctacggtga gccattctat acacgcttaa atttacgcta tgaattctaa
     5821 ttccgtataa caagcggtca aaaaattaaa aaaatttgca attttaggca gtaatcggta
     5881 aggttactgc ctaagtgatt ttattatttt tagtttaatt gaggttttca aatgaaagtg
     5941 attttattag gtgcgccggg tgcgggcaaa ggtacgcaag ctcagtttat tatgaacaaa
     6001 ttcggtattc cgcaaatttc aaccggcgat atgttccgtg cagcgattaa agaaagcact
     6061 gagcttggta aacaagcaaa agcattgatg gacgaaggca agttagtgcc ggatgaatta
     6121 acggtcgcct tagtaaaaga tcgtattgct caaccggatt gtgctaatgg cttcttatta
     6181 gacggtttcc cacgcaccat tccgcaagct gatgcgttaa aagagtcagg tgtaaaaatt
     6241 gatttagtgt tagagtttga tgtagctgat gaagtcattg ttgagcgtat gagtggtcgt
     6301 cgtgttcacc aaccttcagg gcgtacttac cacgtggtgt acaatccacc gaaagtggaa
     6361 ggcaaagatg atgtgactgg tgaggattta attatccgcc aagatgacaa acctgaaacg
     6421 gtattagagc gtttagcgat ttaccacaaa caaaccaaac cgttaatcgc ctactacacc
     6481 gcagaagcag aagccggcaa cactcgttat gagcgtttag atggaacaaa accagtggaa
     6541 gaagtaagtg ctgagttagc gaaaatttta tcttaaatgg attattaaaa tgaacaagcg
     6601 gtaatatttt tcgagaatat taccgcttgt tctttgctta aacacaatat ttggtgagca
     6661 ttaagatatc atcataaatc ggtgaaataa acacttccgg attttcaatc ttcatctcct
     6721 ttggtttttc ttctagcttt tctggctctt tcttatcttt attctctacc ggttgagtcg
     6781 cttcatttac cacactagct tgagttttat ctgctacagg tatatctatt ttgtttggtg
     6841 cttgctctac tgcttgagaa tccgctttca gctctttaga aacttcagta tttacgatag
     6901 gttcttgtaa gttttccgct actctttcct ctacttgtgg cttaatactt tctgcctctg
     6961 ctctcggttt ataagatgga tcaagtaatt gatggctaat atccattttc ggatcaagcc
     7021 aaattgctac aatagcatca ttgaaagctt tattaaacac atcagagact actttatcat
     7081 ttaaaataaa ataacctcta tcttctaatg caatatagtg caatgtatct tcaggcttaa
     7141 tatctgggaa agtttttctc aggtgggtaa taacctcatc taactctttt tcaggtagct
     7201 taatgcctaa attattccaa gttcttgcta aagaaaccgc aaaatcacgc ccctcccacg
     7261 gttttttata ttgtagagta agcattaaag gacgtgtacc ttctacataa gtaccgctct
     7321 cagtaaaaag cgtaatatgg taaatattaa atggccccca cgtataattc acatcattaa
     7381 tttttgtcca atttgcattt gctgttgata aaaatagaga aaacaacagc gaaaagacca
     7441 ctttatagct cattattttt gtgtccttaa atctaaaatt cctactcatt attat
//
LOCUS       DANLYI010000117         7484 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.117, whole genome shotgun sequence.
ACCESSION   DANLYI010000117 DANLYI010000000
VERSION     DANLYI010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 7484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7484
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.117"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            complement(181..1116)
                     /gene="ftsX"
                     /locus_tag="PXD81_001706"
     CDS             complement(181..1116)
                     /gene="ftsX"
                     /locus_tag="PXD81_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsX"
                     /protein_id="HDL5537713.1"
                     /translation="MIRSFSKLGAQTNYSLRAVWQDLMQRKLGTFLTVLVIAVSLTIP
                     TVSYLLWKNTHEAATQFYPEPQLTVYLHKNLAEHDVNTVVDRIRAFETDKIESFNYIS
                     RQQSLDEFRSWSGFGEALDILDENPLPAVVTLKPKANFNNADTMVELRNGLQQIKGVQ
                     EVRLDNGWLEKLTALTWLIARIAVVCTLLMLLAVFLVIGNSVRTDVANSKAAIEVQQI
                     LGATDHFIARPFLYTGMIYGLFGSLLAVLFSAVTISYFTGVVKYVTDMFTVQFELNGL
                     DFSEAFFLVVLCVFIGWLSAKIATMRNIFRIGSRG"
     gene            complement(1126..1779)
                     /gene="ftsE"
                     /locus_tag="PXD81_001707"
     CDS             complement(1126..1779)
                     /gene="ftsE"
                     /locus_tag="PXD81_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015674492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HDL5537714.1"
                     /translation="MIKFTNVNKVYKGGKPALQGIDFHLPAGGMAYITGHSGAGKSTL
                     LKLIMGIERANGGQILFNGHDITRLAHHELPFLRRQIGMVHQDYRLLTDRTILDNVAL
                     PLIILGMNQAIVEREARIALERVGLADKANYLPLHLSGGEQQRVDIARAIVHRPTLLL
                     ADEPTGNLDEKLSFEIFRLFEEFNQAGTTVLIATHDTNIISKRPKPCLVLEQGHLKF"
     gene            complement(1772..3268)
                     /gene="ftsY"
                     /locus_tag="PXD81_001708"
     CDS             complement(1772..3268)
                     /gene="ftsY"
                     /locus_tag="PXD81_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HDL5537715.1"
                     /translation="MSEKKKGFWSWLGFGKKEEAEQKEIQQEQESIQPEQPVIVEPET
                     EKSFSEKLDELEDKIEEKVDQLEDYFEEKEEKIEQFAEQVEDTIEQKVEDFKEFVEEK
                     VEEAKAFFDDVEDKIEQTFEEQKGEQAVENLQNIATEQLDLNEQEQVTYQVEEAVAEE
                     VAETELVNNVQDTVEESTIQDEYQEKPSQGGFFSRLIQGLIKTKQNIGSGFRNFFRGK
                     KIDDELFEELEEQLLVADLGMPTTQKIINNLTQHATKQQLKDADLLYQQLKVELGEVL
                     KPVAQPLVLEAKKPYVILMVGVNGVGKTTTIGKLARKFQQEGKSVMLAAGDTFRAAAV
                     EQLQVWGERNNIPVVAQSTGSDSASVIFDAMQSAAAKGIDVLIADTAGRLQNKNNLMD
                     ELKKIVRVMKKYDETAPHEIMLTLDAGTGQNAISQAKLFNEAVGLTGITLTKLDGTAK
                     GGVIFAIADQFNIPIRFIGVGEKIEDLRPFNAEEFIEALFSHEDEQND"
     gene            3347..3937
                     /gene="rsmD"
                     /locus_tag="PXD81_001709"
     CDS             3347..3937
                     /gene="rsmD"
                     /locus_tag="PXD81_001709"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HDL5537716.1"
                     /translation="MKKNRNSPQSVQQMGEVRVIAGLWRGRKLPVLNAEGLRPTTDRV
                     KETLFNWLMHDIAHSRCLDCFAGSGSLGIEALSRQAQAVVFLEKFANAANQLKKNLQA
                     LKSEQGSVIHTDTLAYLAQKNNGEPFNIVFIDPPFHQGFVPQVLNLLAENNWLAPNAI
                     LYVETEKNHPPLELPQGWQILKEKAAGMVVSRLIQL"
     gene            4021..4335
                     /locus_tag="PXD81_001710"
     CDS             4021..4335
                     /locus_tag="PXD81_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciU family protein"
                     /protein_id="HDL5537717.1"
                     /translation="MKKLTPDEAIDIAYDIFLEMAGENLDPADILLFNLQFEERGAVE
                     MVETSEDWDQEIGTLIDPDAFAEVWVGLVNEQDEMDDVFARFLISHDEENREYHVVWK
                     EK"
     gene            4348..4860
                     /gene="hpaC"
                     /locus_tag="PXD81_001711"
     CDS             4348..4860
                     /gene="hpaC"
                     /locus_tag="PXD81_001711"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     reductase component"
                     /protein_id="HDL5537718.1"
                     /translation="MANIDTNQPIEASLKFREAMAHLSSAVSIVTTNGVAGKAGLTVS
                     SVCSVTDSPPTLLFCINKNSHVHDIIQQNGKVCVNVLSHEQEDLAKHFAAILESTMEE
                     RFGWDIWNNGFDDQPVLRSAISALQGNIVDTHAVGTHTIFVVEVSHIDVEPNHSLVYF
                     ARQFRSVNID"
     gene            4978..5850
                     /gene="tehB"
                     /locus_tag="PXD81_001712"
     CDS             4978..5850
                     /gene="tehB"
                     /locus_tag="PXD81_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005601944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase TehB"
                     /protein_id="HDL5537719.1"
                     /translation="MENLICYKQMPVWTKATLPQMFQEKHNTKVGTWAKLTILKGSLK
                     YYELTEEGEIIKEDIFDANHQRPMVEPQLWHKVEALSDDLECQLAFYCKPEDYFAKKY
                     NLTATHSEVLNAVNYTKGGKALDLGCGRGRNALYLNLLGFDVTAVDHNQESIDFLNYM
                     IEKEQAQNITTAVYDINEATIGSQNGEYDLIISTVVMMFLNRERIPAIIENMQKNTKV
                     GGYNLIVCAMSTDDYPCPMPFSFTFKEGELADYYQGWELVKYNEDLGHLHKTDENGNR
                     IQLRFATMLAKKIS"
     gene            5988..7025
                     /gene="purM"
                     /locus_tag="PXD81_001713"
     CDS             5988..7025
                     /gene="purM"
                     /locus_tag="PXD81_001713"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011962107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HDL5537720.1"
                     /translation="MSNTQLSYKDAGVDIHAGNELVERIKPDVKRTRRPEVMGGLGGF
                     GALCALPTKYKEPILVSGTDGVGTKLRLAIDLNKHDTIGQDLVAMCVNDLVVQGAEPL
                     FFLDYYATGKLEVDVATNVVKGIADGCVISGCALVGGETAEMPGMYHEGDYDLAGFCV
                     GVVEKSKIIDGSTVKTGDALIALASSGPHSNGYSLIRKVLEVSRSNPATDLLEDKPLS
                     EHLLAPTKIYVKSILKLVEEVDVHAIAHLTGGGFWENIPRVLPESAKAVIDESSWQWP
                     AIFSWLQEKGNISRYEMYRTFNCGVGMVIALPERDVEKALTLLKESGENAWLIGKIED
                     LGTGSEQVEIR"
     gene            7103..7381
                     /locus_tag="PXD81_001714"
     CDS             7103..7381
                     /locus_tag="PXD81_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HDL5537721.1"
                     /translation="MQLFWPIYFVLMNLAALYLMYSDKQKAIKKEWRVPEITLLSFCL
                     LGGAIGVYLGMKYFRHKTQHWYFHVAVVLSTFAWLIGLPYFYLALSSY"
BASE COUNT         2178 a         1656 c         1438 g         2212 t
ORIGIN      
        1 agctccagct ccagctccag ctccagctcc agctccagct ccagctccag ctccagctcc
       61 agctccagct ccagctccag ctccagctcc acagcaataa tatattgctt atagtaaaaa
      121 accaagttcc taggaactgg tttttatctg caattatttc tctaaaaaca accgcttgta
      181 ttagcctcta ctaccaatac ggaaaatatt tcgcattgtc gcaatttttg ccgacagcca
      241 gccaataaac acacaaagca ccactaagaa gaaggcttcg ctgaaatcta atccgttcag
      301 ttcaaattgt acggtaaaca tatcagtcac atacttcact acaccggtga aatagctaat
      361 cgttaccgca ctaaacagca ccgctaacag gctgccaaac aagccgtaaa tcatacctgt
      421 gtataaaaac ggacgggcaa taaagtgatc agttgcccct aaaatttgtt ggacttcaat
      481 cgctgctttg ctattggcaa catcggttcg cacactgttt ccaatcacta aaaaaactgc
      541 caacagcatt aataaggtac acaccaccgc aatacgagca atcagccaag ttagagcagt
      601 taatttttcc agccagccgt tatctaaacg aacctcctgc acccctttaa tttgttgcaa
      661 accgttgcgt agctccacca tcgtatcagc attattaaaa tttgctttcg gttttaaggt
      721 aacgaccgca ggcaacgggt tttcatctaa aatatctaac gcttcaccaa agcctgacca
      781 agaacgaaat tcgtctaaac tttgttggcg agaaatatag ttaaatgact caattttatc
      841 cgtctcaaaa gcacgaatgc gatcaacaac ggtattcaca tcgtgttctg ccaaattttt
      901 gtgcagataa acggttaatt gtggctcagg gtaaaattgt gttgctgcct cgtgggtatt
      961 cttccaaagt aaataactca cggttggaat cgtaagtgag accgcaatca ccagcaccgt
     1021 cagaaaagtg ccgagcttgc gttgcattaa atcttgccaa accgctctta aagagtagtt
     1081 ggtttgagca cctaatttac taaatgaacg aatcataata tttccttaaa atttaagatg
     1141 cccttgctcc agcactaaac acggctttgg acgctttgaa ataatattgg tatcgtgggt
     1201 ggcaatcagc accgttgtgc cggcttgatt aaattcctca aacaaacgga aaatttcaaa
     1261 cgagagcttt tcatctaagt ttccggttgg ctcatcggct aataacagcg ttgggcgatg
     1321 aacaatggcc ctggcaatat cgacacgttg ttgctcaccg ccggaaaggt gcaacggcag
     1381 atagttcgct ttatctgcca agccaacacg ctcaagggca atgcgggctt cacgctccac
     1441 aatcgcttgg ttcattccca aaataatcaa tggtaatgcg acattatcca aaatggttcg
     1501 gtcggttaat aagcgataat cttgatgcac catcccgatt tggcggcgta aaaacggcaa
     1561 ttcatgatga gcaagtcggg taatatcgtg tccgttaaac aaaatttgcc cgccattggc
     1621 acgctcaatc cccataatta attttaatag cgtgcttttc cccgcaccgg aatgcccggt
     1681 aatatatgcc attccaccgg caggtaggtg gaaatcaatg ccctgtaagg ctggctttcc
     1741 gcccttataa accttgttca cattagtgaa tttaatcatt ctgttcgtcc tcgtggctaa
     1801 ataaggcttc aataaattct tctgcattaa acgggcgtag atcttcaatt ttctcgccca
     1861 ccccaataaa gcggatcgga atattgaatt gatcggcaat cgcaaaaatc acgccacctt
     1921 ttgccgtacc gtctaattta gttaaggtga taccggttaa acccactgcc tcattaaaca
     1981 atttcgcttg gctgatcgca ttttgcccag tgccggcatc aagcgtaagc ataatttcgt
     2041 gcggtgcggt ttcatcatat tttttcatca cccgcacaat tttcttcaac tcgtccatca
     2101 ggttattttt gttttgcaaa cgccctgcag tatcagcgat tagcacgtca atcccttttg
     2161 ccgcagcgga ttgcatcgca tcaaaaatca ccgaggcaga atccgaaccg gtgctttgtg
     2221 ccaccaccgg aatgttattt cgctctcccc aaacctgtag ctgttcaact gccgccgctc
     2281 ggaacgtgtc tcctgctgct aacattaccg atttgccttc ttgttggaat ttacgggcaa
     2341 gtttgccaat ggtagtggtt ttccccacgc cattcacacc aaccattaaa atgacatacg
     2401 gttttttcgc ttccaacact aacggttgag ccaccggttt tagcacttca cccagttcca
     2461 ctttcaactg ttgatacaac aaatctgcat cttttaattg ttgcttggta gcatgttgcg
     2521 ttaaattgtt gatgattttt tgtgtggtcg gcatacctaa atccgccaca agcaactgct
     2581 cttccagctc ttcaaataac tcatcatcaa ttttcttgcc acggaagaaa ttacggaagc
     2641 ccgaaccaat attctgcttg gttttaatca agccttgaat taaacgactg aagaaaccgc
     2701 cttgactcgg tttttcttga tattcatctt ggatagtaga ttcttcaact gtgtcttgaa
     2761 cattatttac taattcagtt tcagcaactt cttcagccac agcctcttcg acttgataag
     2821 taacttgctc ttgttcgttg agatctaact gctcagttgc aatattttga agattttcga
     2881 ccgcttgttc gcctttttgt tcttcaaatg tttgttcgat tttatcttca acatcatcaa
     2941 agaacgcttt cgcctcttca actttttctt caacaaactc tttgaaatct tcgacttttt
     3001 gttcaatggt atcttctact tgctcagcaa attgctcaat tttttcttct ttttcctcaa
     3061 aatagtcttc taactgatct accttttctt caattttatc ttccagctca tctaattttt
     3121 cagaaaacga cttctcagtt tccggttcaa caataacagg ctgttccggt tgaatactct
     3181 cttgttcttg ttgaatctct ttctgttcag cttcttcttt cttaccaaat ccaagccaag
     3241 accaaaaccc ttttttcttc tctgacatat ttttctctca tttctcgtaa aaatagacta
     3301 aacttacata gtctatcaaa aaatccctta ttttccttta gtgagtatga aaaaaaatcg
     3361 aaattccccc caatctgttc agcaaatggg cgaagtacgg gttatcgccg gactgtggcg
     3421 aggcagaaaa ttacctgtac taaatgccga ggggcttcgc ccgacaaccg atagagtaaa
     3481 agaaacgctg tttaactggt taatgcacga cattgctcac tcacgctgtc ttgattgctt
     3541 tgcaggcagt ggctctctgg gcattgaagc cctttcccgc caagcacaag cggtcgtttt
     3601 tttggaaaaa tttgcaaatg ctgccaacca gttaaagaaa aatctccaag cattaaaatc
     3661 cgaacaaggt agcgttattc acactgacac cctcgcctat ttagcacaaa aaaataacgg
     3721 tgagccattt aacatcgtat ttatcgatcc gccttttcat caagggtttg tacctcaagt
     3781 gctaaacttg ctggcagaaa ataactggct cgccccgaat gcgattttgt atgtggaaac
     3841 cgagaaaaat cacccgccat tagagctacc gcaaggctgg caaattttaa aggaaaaagc
     3901 agccggaatg gtggtaagcc gtctgattca gctataatct ttttgttttt tataactaaa
     3961 aaatataaaa taaaacttat ctatctaaca ttttattaac actcaaatta gaataacgaa
     4021 atgaaaaaat taacccctga tgaagccatt gatattgcct acgatatttt cttagaaatg
     4081 gcaggcgaaa atttagatcc tgccgatatt ttactgttca acctccaatt tgaagagcgt
     4141 ggtgcagtgg aaatggtcga aacttctgaa gactgggatc aagaaatcgg gactttaatt
     4201 gaccctgatg cctttgccga agtttgggtt ggcttagtca atgaacaaga tgaaatggac
     4261 gatgtattcg cccgtttctt aatttcccac gatgaagaaa accgtgaata tcacgttgtt
     4321 tggaaagaaa aataaaaagg agaaaaaatg gctaatatcg acactaatca accaatagaa
     4381 gcctcgctta aatttcgtga agcaatggct cacttatctt ctgcggtgag cattgtgaca
     4441 accaatggcg tagccggcaa agcagggctg acggtttcgt ctgtttgttc cgttaccgat
     4501 tccccaccaa cgctcttatt ttgcattaat aaaaacagcc acgttcacga tattatccaa
     4561 caaaacggca aagtatgcgt taatgtgtta agccacgaac aagaagactt agcaaaacat
     4621 tttgccgcca ttttagaaag tacaatggaa gaacgctttg gttgggatat ttggaacaac
     4681 ggctttgacg atcagcctgt gttacgcagt gctatttctg ctttacaagg caatattgtt
     4741 gatacgcacg cagtgggtac gcatactatt tttgtggtag aagtgagcca tattgacgta
     4801 gagccgaacc acagtttagt ttattttgcc cgccaattta gaagcgtgaa cattgactaa
     4861 aatatattta acagaaattt gatcaaatcc gactttttta gtcggatttt ttatttcctg
     4921 tttttaaaac agtgctaaaa ttttgccgtt tatgtttgac tgacaaaagg aaacaaaatg
     4981 gaaaatttaa tttgctacaa acaaatgccg gtgtggacaa aagcaacctt accacaaatg
     5041 ttccaagaaa agcacaacac caaagtcgga acttgggcaa aactcaccat tctcaaaggt
     5101 tctctcaaat attatgaatt aaccgaagag ggagaaatta tcaaggaaga tatttttgat
     5161 gcaaaccacc aacgcccaat ggttgagcct caactttggc ataaggttga agctctttct
     5221 gacgatttag aatgccagtt ggctttctat tgcaagccgg aagattattt tgctaaaaaa
     5281 tataatctga ccgctaccca ttcagaagtg ctaaatgccg tcaattatac caaaggtggc
     5341 aaagcgttag atttaggttg cgggcgagga cgcaatgcac tttatctgaa tttactgggt
     5401 tttgatgtca ctgctgttga tcataatcaa gaaagcattg attttctcaa ctatatgatc
     5461 gaaaaagaac aagctcaaaa cattactacc gctgtttatg atattaacga ggcaactatc
     5521 ggctcgcaaa acggtgaata tgacctgatt atttccactg ttgtaatgat gttcttaaac
     5581 cgtgaacgca ttcctgccat tattgagaat atgcagaaaa acactaaagt cggcggttat
     5641 aacttaatcg tatgtgcaat gagtaccgat gactaccctt gcccgatgcc gttctccttt
     5701 accttcaaag aaggcgagct cgccgattat tatcaaggtt gggaattagt caaatataac
     5761 gaagatttag gacatttaca caaaactgat gaaaacggca accgcattca actacgtttt
     5821 gcgacaatgt tggcgaaaaa aatcagctaa acttaaccgc ttgcaggggg aattagcttc
     5881 cccctaattc atcaaatcca ttctatttat tttcactttt tcattccgca aacgtttgct
     5941 tgtgctataa tggcttcgtt ttttagtcat ttactgttga ggttaaagtg agcaacacac
     6001 aactaagtta caaagatgcc ggcgttgata ttcacgcagg aaacgagtta gtcgaacgaa
     6061 tcaagcctga tgttaaacgc acccgtcgcc ctgaagtaat gggcggatta ggtggttttg
     6121 gtgctttatg tgctttaccg acaaaatata aagagccgat tttagtttcc ggtaccgatg
     6181 gcgtcggcac aaaattacgc ttagccattg acttaaacaa acacgatact atcgggcaag
     6241 atttggtagc gatgtgtgtg aatgaccttg tcgtgcaagg tgctgaaccg cttttcttct
     6301 tagattatta cgccaccggt aaattagagg ttgatgtagc caccaatgtc gtcaaaggca
     6361 ttgcagacgg ttgcgtgatt tcaggttgtg ctttagtggg aggtgaaacg gctgaaatgc
     6421 cgggaatgta ccacgaaggc gattacgatt tagccggatt ctgtgtcggt gtggtagaaa
     6481 aatctaaaat cattgacggt tcaacggtga aaaccggtga tgccttaatt gcattagcgt
     6541 ccagtggccc gcattccaac ggctattcgt taattcgtaa agtattggaa gtcagccgat
     6601 ccaatcctgc aaccgattta ttggaagaca aacccttaag cgaacactta ttagccccaa
     6661 ctaaaattta tgtaaaatca atcctaaaat tagttgaaga agttgatgtt cacgctatcg
     6721 ctcacttaac cggtggcggt ttctgggaaa atattccacg cgtattgccg gaatctgcca
     6781 aagcggtgat tgatgaatcg agctggcaat ggccggcgat atttagctgg ttacaggaaa
     6841 aaggcaatat cagccgttat gaaatgtacc gtactttcaa ctgcggtgtc ggaatggtga
     6901 tagccttacc tgagagagat gtcgaaaaag cccttactct tcttaaagag agtggtgaaa
     6961 atgcgtggct tatcggtaaa attgaagact taggcaccgg tagcgagcaa gttgaaatcc
     7021 gctaatttgc aaaataaatg ctaaaattga ccgcttgtag taactgatct gcaaacggtt
     7081 aaatttttat tgaggaatgc aaatgcaact attttggcct atctactttg tcttaatgaa
     7141 cctagctgcc ttatatttaa tgtatagtga caaacaaaaa gcgatcaaaa aagaatggcg
     7201 agtgccagaa attactctcc tctctttttg cctcttaggt ggggctatcg gtgtttacct
     7261 aggtatgaaa tactttcgcc ataaaaccca acattggtat ttccacgtcg ctgttgtgct
     7321 ttctactttt gcttggctga ttggcttgcc ttacttttat ttagctttat catcatatta
     7381 atttatcatt acttttttta atccgaaagc ccaatttata tcctagtccg ctcacaaaaa
     7441 atttgattta gatcgcatta tttgtctatt aaaatcgtta ttat
//
LOCUS       DANLYI010000118         7448 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.118, whole genome shotgun sequence.
ACCESSION   DANLYI010000118 DANLYI010000000
VERSION     DANLYI010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 7448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7448
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.118"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            complement(<1..796)
                     /gene="cysQ"
                     /locus_tag="PXD81_001715"
     CDS             complement(<1..796)
                     /gene="cysQ"
                     /locus_tag="PXD81_001715"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HDL5537722.1"
                     /translation="MQTLTQPLLENIINIAKAAGEQLKQLYARSVAIKIKADCTPVTE
                     ADLIISQFLTERLKQLTPDIPVLSEEFCDIPFAERQQWQEYWIIDPIDGTQQFINRTG
                     QFSVVIALVQKREGRNQPVLGVIHAPILGKTYFSMEGSGSFLQENGEIRPLVKPKRAE
                     NALLIVTVGSDLHAEQAKNAISAPQIECVKYGSGSLKAGLVAEGKADCYIRFGDTGEW
                     DTAVAEVLLSEMGGKIFDFNFSPLTYNQRESLINPYFVMVADGSIDW"
     gene            complement(810..1352)
                     /gene="nudE"
                     /locus_tag="PXD81_001716"
     CDS             complement(810..1352)
                     /gene="nudE"
                     /locus_tag="PXD81_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HDL5537723.1"
                     /translation="MIKQLPEILNIKTVAKSRIFEVQAVDLRFSNGEERTYERLTPNR
                     RSSVMVIPIQNNELIFVKEYAVGSERYELGFPKGIVDAGEEPIESANRELQEEIGFGA
                     KKLDFLRSLYSSPSHMYGLMHLFLAQDLYPSKLEGDEPEPLEIVRYPLDKIDQLLADP
                     DFAEARNLSALFMLREYLKK"
     gene            1440..1856
                     /gene="hslR"
                     /locus_tag="PXD81_001717"
     CDS             1440..1856
                     /gene="hslR"
                     /locus_tag="PXD81_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HDL5537724.1"
                     /translation="MKNKHEKEQNDAEVRLDKWLWAARFYKTRSIAKAMIEGGKVHYN
                     GQRAKVSKAVEIGAIIKLRQGNEEKEVEVLALSDQRRGAPEAQLLYSETEKSIKHREA
                     MAFARKANALSMPHPDRRPNKKERRDLIKFKEQEFS"
     gene            2100..2993
                     /gene="hslO"
                     /locus_tag="PXD81_001718"
     CDS             2100..2993
                     /gene="hslO"
                     /locus_tag="PXD81_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HDL5537725.1"
                     /translation="MNYTKDNDKLYRYLFKDRAVRGEWVRLNNTFTETLNTHHYPKAV
                     QNLLGEMLVATSLLTATMKFNGNITVQIQGDSPLKLAVVNGNDQQQLRALARVQADIA
                     DTASLAEMIGNGVLVISIIPTDGERYQGVIALDKPTIRECLEDYFARSEQLQTHLVIR
                     TGEFEGKAVAGGMLLQIMPDGTGSRDDFEHLATLTETVKDEELFGLEAEELLYRLYHE
                     EQVEVYPPQDTEFKCGCSRERSGSALLLVPAEEIEEMLEEKQGVIDMQCECCGTQYFF
                     DKAAIEGFKEETEKLSKLGLN"
     gene            complement(3067..3705)
                     /locus_tag="PXD81_001719"
     CDS             complement(3067..3705)
                     /locus_tag="PXD81_001719"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015431925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HDL5537726.1"
                     /translation="MQNKIDWQKVDGLLPVIVQNAQTCEVLMLGYMNQQALEKTLAEK
                     RVTFYSRTKQRLWTKGETSGNFLNVVDMSLDCDNDTLLILANPIGETCHTGAESCFHQ
                     FEQEQPDWIFFSKLERMIAERKNADPESSYTAKLYAKGTPKIVQKVGEEAVESVIAAM
                     KNDREELVGEVADLMYHLTVLLHNADLSWAEINAKLKERHKGIGLHPEGSNK"
     gene            complement(3733..4119)
                     /locus_tag="PXD81_001720"
     CDS             complement(3733..4119)
                     /locus_tag="PXD81_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDL5537727.1"
                     /translation="MIKITALDHLVLTVADMQKTIDFYTTILGMQEITFGDNRKALLF
                     GTQKINLHQKGAEILPNAQNADCGTADLCLLTVTPLQEVIYVLQRHQVEILEGGIVPR
                     TGAVGKIESVYCRDPDGNLIEISLYV"
     gene            complement(4116..4889)
                     /gene="hisF"
                     /locus_tag="PXD81_001721"
     CDS             complement(4116..4889)
                     /gene="hisF"
                     /locus_tag="PXD81_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005624689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HDL5537728.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAQRYAQEGA
                     DELVFYDITASSDSRTVDKSWVERVAQVIDIPFCVAGGIKTIADAEQIFAFGADKISI
                     NSPALANPELITELAKRFGVQAVVVGIDSWFEAETGKYWVNQYTGDEKRTRQTEWQLL
                     DWVEEVQKRGAGEIVLNMMNQDGVRNGYDLVQLKKVREVCQVPLIASGGAGEMVHFRD
                     AFIEANVDGALAASVFHKQIINIGELKEYLAKEGVEVRR"
     gene            complement(4871..5620)
                     /gene="hisA"
                     /locus_tag="PXD81_001722"
     CDS             complement(4871..5620)
                     /gene="hisA"
                     /locus_tag="PXD81_001722"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006995165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HDL5537729.1"
                     /translation="MQKSIIIPALDLIDGQVVRLHQGDYAKQTTYADDPIAQFADYLA
                     QGAEQLHLVDLTGAKNPAQRQTALIGKIIRETHCKIQVGGGIRTEQDVADLLAVGANR
                     VVIGSTAVKEREMVKGWFKKYGAEKFVLALDVNIDAIGQKIIAMSGWQEASGVSLEEL
                     IEDYQTVGLQHVLCTDISRDGTLAGSNVDLYREICAKYPEIQFQSSGGIGSLADIKAL
                     CGTGVAGVIVGRALLEGKFNVAEAIECWQNG"
     gene            complement(5790..6635)
                     /locus_tag="PXD81_001723"
     CDS             complement(5790..6635)
                     /locus_tag="PXD81_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor protein"
                     /protein_id="HDL5537730.1"
                     /translation="MNEIKLFENSQIRSVWDSEKEEWFFSVVDIIEVLTQSSNPRRYW
                     SDLKRKIKDEEGGIELYEKIVQLKLKAPDGKMRETDATDLQGIFRIIQSVPSPKAEPL
                     KMWLAEVGKERIDEIIDPELTIDRALATYLKKGYSREWINQRLQAIQVRKELTDTWQD
                     HGVQVGREFAILTNEITQAWSGMTTRQYKDFKGLKKESLRDNMTTTELVLNMLAEAAT
                     KDIAQISHPQGLEENKTVAKRGGNVAKVARESLEAETGKPVITQQNAIDFAAVIEQVA
                     KQVKK"
     gene            complement(6734..7327)
                     /gene="hisH"
                     /locus_tag="PXD81_001724"
     CDS             complement(6734..7327)
                     /gene="hisH"
                     /locus_tag="PXD81_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015431920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HDL5537731.1"
                     /translation="MTNLTIIDTGCANLSSVKFAFDRLGIRAEISRDLNKIQSADKLL
                     LPGVGTAAAAMAILHDRRLIDTIRNATQPMLGICLGMQLMTDFSHEGDVATLNLMSGK
                     TERLPECGLPLPHMGWNKVQYQADHPLFHDIEQGNHFYFVHSYGVLPNENTIATCDYG
                     VPFSAAIASQNFYGVQFHPERSGEAGAKLLRNFVENV"
BASE COUNT         1959 a         1848 c         1556 g         2085 t
ORIGIN      
        1 cccaatctat cgagccgtca gccaccatca caaaataagg attaatcaaa ctttctcgct
       61 gattataagt cagcggcgaa aaattaaagt cgaaaatttt gccgcccatt tcacttaaca
      121 gcacttctgc cacagcagta tcccattcgc cggtgtcacc aaaacggata taacaatcgg
      181 ctttcccttc tgccactaaa ccggctttta gactgcccga accatatttt acacactcaa
      241 tttgtggggc tgaaatggca tttttagctt gttctgcatg taaatcgctg cctacggtaa
      301 caatcaaaag ggcattctcc gcacgtttcg gtttgacaag cggtcgaatt tctccatttt
      361 cttgcaaaaa gctaccgcta ccttccatac taaaataggt ttttcctaat atcggagcat
      421 gaataacgcc cagcaccggc tgatttctac cctcacgttt ttgcactaac gcaatcacca
      481 ctgaaaattg accggtgcgg ttaataaact gttgtgtgcc gtcaatcggg tcgataatcc
      541 aatattcttg ccattgttgg cgttcggcaa acggaatatc gcaaaactct tccgataata
      601 ccggaatatc tggtgtgagt tgcttcaagc gttcagttaa aaattggctg ataatcagat
      661 ccgcttccgt caccggtgtg caatcggctt tgattttaat tgccaccgag cgggcataaa
      721 gctgctttaa ctgctcaccg gctgctttgg caatattgat aatattctcg agtaggggtt
      781 gagttagcgt ttgcataata gctccttgcc tattttttta agtattctcg tagcataaat
      841 agagcggaca aattccgtgc ttcggcaaaa tcaggatctg ccaataattg atcaatttta
      901 tccaacggat aacgcacaat ttccagcggt tcaggctcat cgccttccaa ttttgatggg
      961 tacagatctt gtgccaaaaa caggtgcatt aagccgtaca tatggcttgg cgaactgtaa
     1021 agcgaccgca gaaaatctaa ttttttcgcc ccaaaaccaa tttcttcttg taattcacgg
     1081 ttcgcactct caatcggctc ttcacctgca tccacaatgc ctttcggaaa acccagctcg
     1141 taacgctccg agccgaccgc atactctttc acaaaaatca gctcgttgtt ttgaatcgga
     1201 atcaccatta ccgaagaacg gcgattcggc gtaagtcgtt cgtaagttcg ctcttcgcca
     1261 ttagaaaagc gtaaatcgac cgcttgcacc tcaaaaatgc gagatttcgc tactgtttta
     1321 atgttcaaaa tttcaggaag ttgttttatc attttatctc acctttttga gttattttag
     1381 ttgaaaaatc tcactttcgc tctaatattt cgactattgt tttgatttta aggcagaaaa
     1441 tgaaaaataa acacgaaaaa gaacaaaacg acgccgaagt tcggctagat aaatggcttt
     1501 gggcggctcg tttttataaa acccgttcga ttgccaaagc aatgattgaa gggggtaaag
     1561 tccattataa tgggcaaaga gctaaagtta gcaaagcggt ggagatcggt gccatcatca
     1621 aacttcgcca aggcaatgaa gaaaaagagg tggaagtgtt agccctcagt gaccaacgcc
     1681 gaggggctcc ggaagcccaa ctgctctaca gtgaaaccga aaaaagcatt aaacaccgtg
     1741 aggcaatggc atttgcccgc aaagcgaatg ctctttcaat gccacaccct gatcgccgtc
     1801 caaacaaaaa agaacggcgg gatttaatca aattcaaaga gcaagaattt agctaagccc
     1861 ttgcaatcag aaaaataatc cctatattga atatcctaag taggggcgaa aaatttttcg
     1921 cccctatata attatttcgg gcgaacattg attcgcccct acagcgttcg atttgcacat
     1981 ttttagccac aaaatgcaat gcattttgaa cgttggcttt gccaacggct ccgtaggagc
     2041 gaacaaaatt agaaataatt ttgtgagtaa taatcgaccg cttgcaacga ggaatcaaaa
     2101 tgaattacac caaagacaat gacaaactct accgctattt attcaaagac cgagcggtac
     2161 ggggcgaatg ggtgcgttta aacaacacct ttaccgagac cttaaacacc catcactacc
     2221 ctaaagcagt acaaaattta ctcggagaaa tgttagtcgc aaccagtttg ctgactgcaa
     2281 caatgaaatt taacggcaac attactgtgc agattcaagg tgacagcccg ttaaaattgg
     2341 cggttgtgaa cggtaacgac caacagcaac tccgtgcttt agcccgtgtt caggcagata
     2401 ttgcagacac agcctcgtta gcggaaatga tcggcaatgg tgtattagtg atttctatta
     2461 tcccaaccga tggtgaacgt tatcaagggg tgattgctct cgataaacca accattcgtg
     2521 aatgtttaga agattacttc gcccgctccg agcaattaca aacccacctc gttatccgca
     2581 caggcgaatt tgaaggcaaa gcagtggcag gcggtatgtt gctacaaatt atgccggacg
     2641 gcacaggctc aagagatgat tttgagcatt tagccacgct cactgaaacc gtaaaagatg
     2701 aagagttatt tggtttagag gcagaagaat tgctctatcg cctctaccac gaagagcaag
     2761 tagaagttta cccaccacaa gatactgaat ttaaatgtgg ctgctcaaga gagcgttcag
     2821 gctccgcatt attactggtg ccggcagaag aaatcgaaga aatgttggag gaaaaacaag
     2881 gcgtgatcga tatgcaatgt gaatgctgcg ggacacaata tttctttgac aaagcggcga
     2941 ttgaaggctt taaggaagaa actgaaaaat taagtaagtt agggttaaat taagctaaac
     3001 aagcggtcaa gtttcttgtc ttttttgcaa tttgtaaaaa aacggtgaaa tttgaccgct
     3061 tgcattttac ttatttgagc cttctgggtg taacccaatc cctttgtggc gttcttttag
     3121 tttagcattg atctctgccc acgagagatc ggcattgtgc aataacaccg ttaagtgata
     3181 catcagatca gccacttcac cgaccaactc ttcccgatca tttttcattg ccgcaatcac
     3241 gctttctact gcctcttccc ctactttttg gacgattttt ggcgtgcctt tggcgtaaag
     3301 tttagcggta taagaacttt ccggatcagc atttttacgt tcggctatca ttcgttctaa
     3361 tttgctgaaa aagatccaat ctggttgttc ttgttcaaac tgatgaaaac agctctccgc
     3421 ccccgtatgg caggtttcgc cgatagggtt agcgaggatt aacagggtgt cgttatcaca
     3481 atccaaactc atatccacca cgtttaggaa attgcccgag gtttcgcctt tcgtccacag
     3541 ccgttgttta gtacgggagt aaaaagtcac tcgcttttca gccagcgttt tttcgagggc
     3601 ttgttggttc atatatccta acatcagtac ttcgcaggtt tgggcgtttt ggacgataac
     3661 gggcagtaag ccgtccactt tttgccagtc tattttattt tgcatttttt cttccttttt
     3721 ttgaccgctt gtttagacat ataggcttat ttcaatcaaa ttaccatcag ggtcacggca
     3781 ataaaccgac tcaatttttc ccactgcccc cgtacgaggc acaatcccac cttccaaaat
     3841 ctccacctga tgtcgctgta acacatagat aacctcctga agtggagtta ccgttaagag
     3901 gcataaatct gccgtgccac aatcggcatt ttgggcattg ggtaggattt ctgcaccttt
     3961 ttggtggagg ttgattttct gtgtgccaaa caaaagcgct ttgcgattat cgccaaaggt
     4021 gatttcttgc atacccaaaa tggtcgtata aaaatcaatg gttttttgca tatcggcaac
     4081 ggttaaaacc agatgatcta aggcagtaat cttaatcatc tacgcacctc aaccccttcc
     4141 ttcgccaaat attccttcaa ctccccaata ttgataattt gtttatgaaa cacactcgct
     4201 gccaacgcac catccacatt tgcctcaata aaagcatcac ggaaatgcac catttcgcct
     4261 gcaccgcctg aggcgatgag tgggacttgg caaacttcac gcactttttt cagttgcacc
     4321 aaatcgtagc cgttgcgtac gccgtcttgg ttcatcatat tcagcacgat ttcgcctgct
     4381 cctcgctttt gcacttcctc cacccaatcc aacaattgcc attcggtttg gcgggtgcgt
     4441 ttttcatcgc cggtatattg attgacccaa tatttgccgg tttctgcctc gaaccagcta
     4501 tcaataccaa ccaccaccgc ctgcacgcca aagcgctttg ccagttcggt aatcagctcg
     4561 gggttggcaa gggctgggga attgatggag attttgtccg ctccgaaagc gaaaatctgc
     4621 tccgcatcgg caatggtttt gattccgcct gccacacaga acggaatatc aatcacctgt
     4681 gccacacgtt ccacccagct tttatccacc gttctgctgt cacttgaggc ggtaatatcg
     4741 taaaacacca actcatccgc cccctcttgg gcataacgct gggcgagtgg cacaatgtcg
     4801 ccgatgattt cgtggttgcg gaactgcacg cctttcacaa cttgcccgtc acgcacgtct
     4861 aaacaaggaa ttatccgttt tgccaacatt cgattgcctc cgctacatta aatttacctt
     4921 ccaacaacgc acgaccgaca atcacgccag ccacgcccgt accacaaagc gctttgatgt
     4981 cggcaagcga gccgataccg cctgaggact gaaattgaat ctctgggtat ttggcacaga
     5041 tttctcgata gagatctaca tttgaacctg ccagcgtgcc gtctcgggaa atgtcggtgc
     5101 ataaaacgtg ttgcaagccc acggtttgat aatcttcaat caactcctcg agcgacacac
     5161 cacttgcctc ttgccagccg ctcatcgcaa tgattttttg accgattgca tcaatgttca
     5221 catccagtgc cagcacaaat ttctccgcac cgtacttttt gaaccagcct ttaaccattt
     5281 cacgctcttt taccgcagtc gaaccaatca ccacacggtt tgctccaaca gcaagtaaat
     5341 ccgccacatc ctgctcggtg cggataccgc cgcccacttg aattttgcag tgggtttcac
     5401 gaataatttt gccgataagt gcggtctgtc tttgagccgg atttttcgcc cctgtaagat
     5461 cgaccaaatg taactgctcc gccccttgtg ccaagtagtc ggcaaattgg gcaatcgggt
     5521 catcggcata agtcgtctgt ttcgcataat cgccctgatg taggcgaact acttgcccat
     5581 cgataaggtc gagggcgggg ataatgatgg atttttgcat attattgttc ctgtgtgcct
     5641 agcacgagcc gtgctaggct gaataatccc agtcgcgctg cgactgcctc tctaagctac
     5701 ggagtagctt ggtttatcta gccccatacg gctcgtatgg ggtacaagcg gtcaaattct
     5761 tccaaaaact tgcaaaaaac cgaccgcact tatttcttca cctgtttcgc cacttgttca
     5821 atcaccgcag cgaaatcaat cgcattttgt tgcgtaatca ccggtttgcc ggtctctgcc
     5881 tccaaacttt cccgagccac ttttgcaaca tttccgcccc gtttggcaac ggttttattt
     5941 tcttccaagc cctgtgggtg gctgatttga gcgatgtctt tggtggcagc ctcagcaagc
     6001 atattcagca ccaactcggt ggtcgtcata ttgtctcgca gactttcctt tttcagccct
     6061 ttgaaatcct tatattggcg agtagtcata ccgctccacg cctgtgtgat ttcattggtg
     6121 agaatcgcaa attctctgcc cacttgcaca ccgtgatctt gccaagtatc cgtcagttct
     6181 tttcgcacct gaatggcttg caagcgttgg ttaatccact cacgggaata tccctttttc
     6241 aagtaagtag ccaaagctcg gtcaatggtc aattcaggat cgataatctc atcaatccgc
     6301 tctttgccta cctctgccag ccacattttc aacggctcgg ctttcgggct tggcacagac
     6361 tgaataatac ggaaaatgcc ttgtaaatcc gtggcatcgg tttcccgcat ttttccgtct
     6421 ggggctttta atttcaactg tacgattttt tcgtacagtt caattccgcc ctcttcgtcc
     6481 ttgattttcc gctttaaatc actccaataa cggcgtggat tgctactttg cgtcagcacc
     6541 tcgataatgt ccaccaccga gaaaaaccac tcttcttttt cgctatccca caccgagcgg
     6601 atttggctgt tttcaaatag tttaatttcg ttcatttctc accttctttc actggttaaa
     6661 ttaacagtaa ttttgtcgaa ataaagcgag aaatcaagcc ctattttcaa acttttcgat
     6721 cgagatcaca aaatcagaca ttctccacaa aattcctgag caacttcgcc ccagcctcgc
     6781 ccgaacgctc ggggtggaac tgtacgccgt agaagttttg gctggcaatg gcagcggaaa
     6841 acggcacgcc gtagtcgcag gtggcgatgg tgttctcatt cggtagcaca ccgtagctat
     6901 gcacaaaata gaaatgattg ccttgctcaa tgtcgtgaaa tagcggatgg tcggcttggt
     6961 attgcacctt gttccagccc atatgcggca acggcaaacc gcattcaggc aagcgttcgg
     7021 ttttgccact cattaagttc agtgttgcca catcgccttc gtgggaaaag tccgtcatta
     7081 actgcattcc taagcagatg ccaagcatcg gctgggtggc gttgcgaatg gtgtcgataa
     7141 gtcggcgatc gtgcaagatc gccatcgccg ccgctgctgt tcccacaccg gggagtagca
     7201 atttatcggc cgattggatt ttgttgagat ctcgcgagat ctcagcccga atgccaagac
     7261 ggtcaaaagc aaatttgacc gaagacaggt tggcacagcc tgtgtcgatg atggtgaggt
     7321 tggtcattta gtttcctgag ttaagtattt cttatagtga agtaaaattg actcaatata
     7381 tctgtttcct tcaaaagctt tcttatcgaa tcttttgata attgccaaaa ataaactatt
     7441 aaagaaaa
//
LOCUS       DANLYI010000119         7412 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.119, whole genome shotgun sequence.
ACCESSION   DANLYI010000119 DANLYI010000000
VERSION     DANLYI010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 7412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7412
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.119"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            41..2188
                     /locus_tag="PXD81_001725"
     CDS             41..2188
                     /locus_tag="PXD81_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HDL5537732.1"
                     /translation="MKRLRYSYLALILGSALPLSAYADGYLSNDNIRQFDARFNFFLA
                     NPLPAPIAKADGRGEAIENLVVKYASDYLNDIYLKEDVIRFRRWQWQDLYTSLDLKTK
                     QKPDYLNTYRLQAEAYFINKQYQEALSQLDQILRRNPNDVHAMAMSVVASRVLENPNT
                     EAERLLALYQISPSIAQKVQQYLHFTDTMLAASYGSEPQTTMIPDTIAVFGQSPNPDG
                     TPSKGLLQRLEKTKEMAEKFPNAKIILSGGPVRYIYAEADVMKKWLIENGVDESRLLL
                     DDVARDTPGNAVGIIDFMKEYGGRKVLGIGTILHLPRAMSVLKSYADSIGYELEIDSA
                     GGGSPPNEKNKKGEALYTFVNVARAMGLFTLGDFENLLAKEQAEKEQREALEKAQQNY
                     LEKLVAERDQAVKNAENSANELEVARQALETAKQAHQQAEEELNKELSKTQEQLKQST
                     EALSRLIIKQNNPFSEHYVVQLNLMEQATYDLYRRLAEIEHTQDWNLWLSDSLSKYRL
                     KNRSTEDYHLLHIGLDHQVSHNWSIGGVFGISQGQIENRTHKVKGTSAGIYAGYRPTS
                     GLFWTNFFNYSHFTNQSGVTKRDNNAYSLSSEIGRNIVMSHQWQITPKLQLGWAKAGS
                     ARMFWAGTGLRLGKVIELNAIKLKPYADISYRFRNRHIDTLDSLSDRHRNVISIGIQA
                     VGNRHYFAIEGSMLHGDALKRSYGLHANYHYQW"
     gene            2201..3319
                     /locus_tag="PXD81_001726"
     CDS             2201..3319
                     /locus_tag="PXD81_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HDL5537733.1"
                     /translation="MKKIKTTLSALALSLSLISVNSWAIPADYLSQDNISQYDKAFNF
                     VLAKVLPAPKGKEDGRGAPIEHLVIQYAPDYVDDPYLKEDVLNFRRWYWQAMYASLDQ
                     KVKEKPTYLNTYRLQAEAYFVNKEYREALSQLDQILRQNPRDIHALAMTVLASRVLED
                     TEQETARLSALKQLAPEVAQRMTDFFAFTEQTLAASYGNEPQTAMIPDTIAVFGSTPN
                     KDGTPSPLLLDRLQKTKEMAQKFPQAKIIVSGGAVKTEFAEADVMAKWLSENGIEKDR
                     LLLDPVARDTPGNAVGIIKLMQTYNGKKVLGIGTLQHIPRATAVLKGYAQHIGYPIEM
                     DSVGGGKPATPGKAKIEALYTYVNVARSMGLFELGDFK"
     gene            complement(3380..4642)
                     /gene="rho"
                     /locus_tag="PXD81_001727"
     CDS             complement(3380..4642)
                     /gene="rho"
                     /locus_tag="PXD81_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HDL5537734.1"
                     /translation="MHLTQLKNTPVSKLVEMGENQMGLENLARLRKQDIVFAILKQHA
                     KSGEDIFGQGVLEILPDGFGFLRSSDSSYLAGPDDIYVSPSQIRRFNLQTGDKIEGKI
                     RPPKEGERYFALLKVDSVNDDKPEASRSKILFENLTPLHANSRLKMERGNGSKEDLTA
                     RILDLAAPIGKGQRALIVAPPKAGKTVLLQNIAQSITHNHPECELIVLLIDERPEEVT
                     EMQRTVRGEVIASTFDEPATRHVQVAEMVIEKAKRSVEHKKDVVILLDSITRLARAYN
                     TVTPVSGKILSGGVDANALHRPKRFFGAARNVEEGGSLTIIATALVDTGSKMDEVIFE
                     EFKGTGNMELHLSRRIAERRIFPAIDFNRSGTRKDDLLMAADELQKTWMLRKVLNPMD
                     EVDAIEWLIDKLMMAKTNEEFFEIMKRS"
     gene            complement(4828..5775)
                     /gene="cysK"
                     /locus_tag="PXD81_001728"
     CDS             complement(4828..5775)
                     /gene="cysK"
                     /locus_tag="PXD81_001728"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015939738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HDL5537735.1"
                     /translation="MTIYADNSYSIGNTPLVRLKNFGNGNIVVKVEGRNPSFSVKCRI
                     GANMVWQAEKDGILTKDKEIVDATSGNTGIALAYVAAARGYKLTLTMPETMSTERKRL
                     LRGLGVNLVLTEGAKGMKGAIAKAEEIAASDPDRYVTLKQFENPANPAIHQQTTGPEI
                     WDATEGKIDVLVAGVGTGGTITGVSRAIKQDKGKQIISVAVEPSESPVITQTLNGEEV
                     KPGPHKIQGIGAGFIPKNLDLSLLDRVEQVSSEEAITTARRLMAEEGILGGISSGAAV
                     AAADRIAKLPEFADKLIVVILPSASERYLSTLLFEGIEA"
     gene            complement(5949..>7412)
                     /gene="pepA"
                     /locus_tag="PXD81_001729"
     CDS             complement(5949..>7412)
                     /gene="pepA"
                     /locus_tag="PXD81_001729"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HDL5537736.1"
                     /translation="KQRTACLVVGVYEPRRLCSAAEQLDKLSNGYISTLLRRGDLEGK
                     AGQTLLLHNVPNVLADRVLLVGCGKERELTERQYKQIIQKSVQTINETGSMEAVSFLT
                     ELHVKGRTPYWNVRFAVEAIQEQLYAYNDFKSIKPEVRRELRRVIFNVAHRKDLPDAE
                     RALEHGKAVALGISHAKNVANCPPNVCNPHYLAELAKGLAYDYPAISTTIVDEKQMAE
                     LGMNAYLAVSRASENEAFLSVIEYKGNPNADAKPIILVGKGMTFDSGGLSIKPAEAMD
                     EMKYDMCGAATVYGTIKAIAEMKLPLNVIGLLAGCENAVDGKAYRPGDILTTMSGLTV
                     EVLNTDAEGRLVLCDALSYVERFEPELVIDIATLTGACMIALGNHNSGLMSPHNGLAN
                     ELLAAAEQADDKAWRLPLGEEYQEQLKSNFADLANIGGRLGGACTAGQFLSNFTKKYT
                     WAHLDIAGTAWKSGAAKGATGRPVSLLTQFLINKAQV"
BASE COUNT         2127 a         1491 c         1615 g         2179 t
ORIGIN      
        1 gttataaata cttcttcacc caatataagg agaaatcata atgaaacgct tgcgttattc
       61 ttatcttgct ttgatattag gttcagcctt acctttatcg gcttatgctg acggttattt
      121 aagtaacgat aatattcggc aatttgatgc ccgttttaat tttttcctcg caaatccact
      181 acctgcgcct attgcaaaag cagatgggcg tggtgaagcg attgagaatt tggtggtgaa
      241 atatgcatca gattatctta atgatatcta tcttaaagag gatgttattc gttttcgccg
      301 ttggcagtgg caagatctct atacgtcatt ggatttaaaa acgaaacaaa agcccgatta
      361 tttaaatact taccgattac aggcggaagc gtactttatc aataagcaat accaagaagc
      421 attatctcaa ctggatcaaa ttctgcggcg taaccctaat gatgttcatg caatggcaat
      481 gagtgtggtt gcaagtcggg tattggaaaa tcctaatact gaagccgagc gtttattagc
      541 gctttatcaa atctctccaa gtattgcgca aaaggtgcag caatatctac attttacaga
      601 cactatgctt gctgccagct atggtagtga gcctcaaaca acaatgatcc ccgacaccat
      661 agcggtattt gggcaatcgc ctaacccaga cggtactcca agcaagggat tattgcaacg
      721 tcttgagaaa accaaagaaa tggcagagaa atttccgaat gcaaaaatta ttttaagtgg
      781 tggacctgtt cgttatatct atgcagaagc ggatgtaatg aaaaaatggt tgatagagaa
      841 tggcgttgat gaaagcagac tgttgcttga tgatgtggca cgagatactc cgggaaatgc
      901 tgtcggtatc attgatttta tgaaggaata tggaggtaga aaagtattgg ggatcggtac
      961 gatcttacat ttaccgagag caatgtctgt attgaaaagc tatgcggatt ccattggata
     1021 tgaattggaa attgattctg ccggaggcgg ttctcctcct aatgaaaaaa ataaaaaagg
     1081 tgaagctctt tatacatttg ttaatgttgc tcgtgcaatg gggttattta ccttgggcga
     1141 ttttgagaat ttgcttgcca aagaacaagc tgaaaaagaa caaagagaag cgttggaaaa
     1201 agcacagcag aattatttag aaaaattggt agctgagcga gatcaagcag taaaaaatgc
     1261 agaaaattct gcaaatgagc tggaagtagc cagacaggcg ttagaaacag caaaacaggc
     1321 tcatcagcaa gctgaagagg aattgaataa agaacttagt aaaacgcaag agcagttaaa
     1381 acagagtacg gaagccctta gtcgtttaat aataaagcag aacaatcctt tttccgagca
     1441 ttatgttgta cagcttaatt tgatggaaca agcaacttat gatctgtacc gtaggttagc
     1501 agaaatagaa catacacaag attggaattt atggctttcg gatagtctat ccaaatatcg
     1561 tttaaaaaat cgttcaaccg aagactatca cttattacat atcgggctag atcatcaggt
     1621 ttcccataat tggtctattg gtggagtatt tggtatcagt caggggcaaa ttgagaatag
     1681 gacgcataaa gtgaaaggga catctgctgg tatttatgct ggttatcgtc ctacaagtgg
     1741 tctgttttgg acaaattttt ttaattacag tcattttact aatcaatctg gtgtaacaaa
     1801 acgagataat aatgcataca gtctgagtag tgaaattgga agaaatatcg tgatgagtca
     1861 tcaatggcaa attacgccaa agctgcaatt aggctgggca aaagctggat cagcacgaat
     1921 gttttgggct gggacagggc tacgtttagg aaaagtgatt gaactcaatg caatcaaact
     1981 taaaccttat gctgatatta gctatcgttt tcgaaatcgt catattgata ccttagatag
     2041 tttatctgat cgtcatcgga atgtaatcag tattgggata caagcagttg gaaatcgcca
     2101 ttattttgca atcgaaggat caatgttaca cggggatgca ttgaagcgtt catacggatt
     2161 acacgcaaat tatcactatc aatggtaaat aggagcctct atgaaaaaaa tcaaaacaac
     2221 cctttctgcc cttgctttaa gcctttcttt gataagtgta aatagctggg cgataccagc
     2281 tgattatttg agccaagaca atatcagtca atatgataag gcgtttaact ttgtattagc
     2341 aaaagtactg cctgcaccaa aaggtaaaga agatggtcga ggagcgccga ttgagcattt
     2401 agttattcaa tatgcgccag actatgttga tgacccttat ctaaaagagg atgtgctgaa
     2461 tttccgccgt tggtattggc aagcgatgta tgcttcgctt gatcagaaag ttaaagagaa
     2521 gccgacctac cttaacactt accgtttaca agcagaggcg tatttcgtca ataaagagta
     2581 tcgggaagca ctttctcagc tcgaccaaat tttgcgccaa aacccccgtg atattcacgc
     2641 tcttgcgatg acagtattag cttcaagggt gctagaagat actgaacaag aaacggctcg
     2701 tctttctgca ctgaaacagc ttgctcccga agtcgcacaa cgaatgacag acttttttgc
     2761 ctttaccgaa caaacgctgg cggcaagcta cggtaatgaa ccacagacag caatgatccc
     2821 tgatacgatt gccgtttttg gttctacgcc aaacaaagac ggcacacctt cgccgctgtt
     2881 gttagatcga ttacagaaaa ccaaagagat ggcacaaaaa tttccgcaag caaaaattat
     2941 cgtcagtggc ggtgctgtga aaacggaatt tgctgaggct gatgtgatgg caaaatggct
     3001 tagcgaaaat ggtattgaaa aagaccgttt gctacttgat cctgttgcca gagatacccc
     3061 gggaaatgcg gttgggatta tcaagctgat gcaaacttat aatggcaaga aagtattagg
     3121 tatcggtacg ttacaacata tccctcgtgc aacagcggtt ctcaaaggtt atgcacagca
     3181 cattggttat ccgattgaga tggattccgt tggtggtggc aagccggcaa caccgggaaa
     3241 agccaaaatc gaggcacttt atacttatgt aaatgtggcg agatcaatgg ggttgtttga
     3301 gttgggggat tttaagtagt tactttattg acagctgcta ttgataaaaa tcccgcgtta
     3361 agcgggattt ttttatgttc tacgaacgtt tcataatctc gaaaaactct tcgttagttt
     3421 ttgccatcat taatttgtcg attagccatt cgatagcatc tacttcgtcc attgggttga
     3481 ggactttgcg taacatccaa gttttttgga gttcgtctgc tgccattaag aggtcatctt
     3541 tacgagtacc tgagcggttg aagtcaatag ccgggaagat acggcgttca gcaatacggc
     3601 gtgagaggtg taattccata ttacccgtac ctttgaactc ctcgaaaatc acttcgtcca
     3661 tttttgatcc ggtatccaca agtgcggttg cgataatggt taagctgcca ccttcttcca
     3721 cattacgggc tgcaccgaag aaacgttttg gacgatgtaa ggcgttagcg tccacaccac
     3781 cggaaaggat tttgccggaa accggggtaa cggtgttgta ggcacgggct aaacgagtga
     3841 tggagtctaa taaaatcacc acatcttttt tatgctctac cgagcgtttt gctttctcga
     3901 ttaccatctc cgcaacttga acgtggcggg ttgccggttc atcaaaggtt gaggcaatta
     3961 cttcaccacg tacggtgcgt tgcatctctg ttacttcttc cggacgctca tcaattaata
     4021 atacgataag ctcacattca ggatgattat gggtgatgct ttgtgcaata ttttgcagta
     4081 acacggtttt accagctttt ggcggggcaa caattaacgc tcgctgacct ttaccgattg
     4141 gggcggctaa atctaaaata cgagcggtta aatcttcttt tgatccattg ccacgctcca
     4201 tttttaagcg agaattggcg tgcagagggg ttaagttttc aaagagaatt ttactgcgag
     4261 aggcttccgg tttgtcatca ttaactgaat caactttcag tagggcaaaa tagcgttctc
     4321 cctcttttgg tgggcgaatt ttaccttcta ttttatcgcc agtttgtaag ttaaaacggc
     4381 ggatttggct tggagaaaca taaatatcat cagggccggc taaatatgag ctatctgatg
     4441 agcggaggaa accgaagccg tccggtagaa tttcaagcac gccttgcccg aaaatgtctt
     4501 caccgctttt tgcgtgttgt ttaagaatag caaatacgat gtcttgttta cgtaaacgag
     4561 ccagattttc taagcccatt tgattttcac ccatttccac aagctttgaa acaggggtgt
     4621 ttttaagttg agtaagatgc ataatttgaa tgtaaaattg tgattaatta agattggatt
     4681 tgttgatatt gattagatac ggccaaccgt aaagatgtgg cgaagaataa cgcaatagga
     4741 gacaaattgc aaatttttta taaaaaataa ccgcttgttt gcaataaaaa tgccgacatc
     4801 agccggcatt tttatattaa tttaatatta cgcttcaatc ccttcaaaca gtagcgtact
     4861 taaataacgc tctgaggctg aaggcagaat cactacaatt aatttatcgg caaattcagg
     4921 tagtttagcg atgcgatctg cggcagcaac ggcagcacct gatgaaatac cgcctaaaat
     4981 gccttcttct gccattaaac ggcgtgcggt tgtaatcgct tcttcgcttg aaacttgttc
     5041 aacacggtca agcagtgata aatctaagtt tttcggaata aagcctgcac caatgccttg
     5101 aattttgtgc gggcccggtt tgacttcttc accgtttaaa gtttgcgtaa tgaccggcga
     5161 ttcactcggt tctaccgcca ctgaaataat ttgcttgcct ttatcttgct taatagcacg
     5221 ggaaacaccg gtaatagtgc cacctgttcc tacgcctgcc actaatacat caattttacc
     5281 ttcagttgca tcccaaattt caggaccggt agtttgttgg tgaattgccg ggtttgccgg
     5341 gttttcaaac tgttttaagg taacatagcg atctgggtcg gaagccgcga tttcttcagc
     5401 tttcgcaatc gctcctttca ttccttttgc cccttcggtt aagactaaat ttactcctaa
     5461 gccacgcaat aagcgtttac gctcggtact cattgtttcc ggcattgtga gcgttaattt
     5521 ataaccacga gcggctgcta cataagccaa ggcaattcca gtgttaccgc tggttgcatc
     5581 tacaatttct ttgtctttgg ttagaatacc gtctttctct gcttgccaca ccatattcgc
     5641 accaatacgg catttcacgc taaaacttgg gttacgccct tccactttta ccacaatatt
     5701 gccgttgcca aaattcttta aacgcaccag cggggtattg ccgattgagt atgagttgtc
     5761 tgcatagata gtcatagtga tattccttaa taagtattga gattgggatt tatcatactt
     5821 atttttaatc gcttttctag tagctggaat ttatttgata taactaaaaa gaatataggg
     5881 gcgtatgaag aggatcggtc tgattttctt tttgcaaaaa ctcgataaaa tcagaccgct
     5941 tgtgagagtt agacctgggc tttattgatc aaaaattgtg ttagtaagga taccggtctg
     6001 ccggtcgccc ctttggctgc cccagatttc caagccgtgc cggcaatatc tagatgcgcc
     6061 caagtatatt tctttgtaaa gttagagagg aattgaccgg cagtacaggc tccgcctaaa
     6121 cgtccgccga tattagcaag atcagcaaag ttagatttta actgttcttg atattcttcc
     6181 cctaaaggta aacgccacgc tttgtcatcg gcttgctctg cggcagctaa gagttcgttt
     6241 gctaaaccgt tatgaggtga catcagcccg ctgttatgat tacctaaggc aatcatgcaa
     6301 gcaccagtta gggtagcaat atcaatcact aattccggtt caaaacgttc tacatagctg
     6361 agagcatcac atagcactaa acgtccttct gcatcggtgt tgagtacctc aacggttaaa
     6421 ccagacattg tagtgagaat atcccccggt cgataggctt tgccatcaac cgcattttca
     6481 cagccggcga gtaagccgat aacattgagt ggtaatttca tctcggcaat tgctttgatt
     6541 gtaccgtaaa cagtagcagc tccgcacata tcgtatttca tttcgtccat cgcctccgca
     6601 ggtttaatgg ataaaccgcc agagtcaaag gtcattcctt tgccgactaa gataatcggc
     6661 ttcgcatcag cattcgggtt gcctttatat tcaatcactg aaagaaaggc ctcattttca
     6721 gaggctctgg aaaccgctaa gtaagcattc attcccaatt ctgccatctg tttttcatca
     6781 acaatggtag ttgaaatggc aggatagtcg taggctaaac ccttagccaa ttcggctaaa
     6841 tagtgaggat tacacacatt aggcggacaa tttgctacat ttttagcgtg gctgattcca
     6901 agtgctacgg cttttccgtg ttctaaagct cgttcggcat caggaagatc tttacggtgt
     6961 gcgacattaa aaatcacacg gcgtaattca cggcgaactt caggtttgat gcttttgaaa
     7021 tcgttatagg catatagttg ttcttggatt gcctctaccg caaatcgcac attccaataa
     7081 ggtgttctac ctttaacgtg cagttcggtt aaaaagctga cagcttccat tgaaccggtt
     7141 tcattaatag tttgtacact tttttgaata atttgtttgt attggcgttc agttaattca
     7201 cgctcttttc cacagccgac taataatact cgatcagcga gtacattcgg cacattatgc
     7261 aagagtaagg tttgccctgc tttgccttct aaatctcctc ttctcagtaa tgtgctgata
     7321 tagccgttac ttaatttatc aagctgttct gcggcagaac ataatcggcg gggttcatag
     7381 acaccaacaa ccaaacaggc ggttctttgt tt
//
LOCUS       DANLYI010000120         7328 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.120, whole genome shotgun sequence.
ACCESSION   DANLYI010000120 DANLYI010000000
VERSION     DANLYI010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 7328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7328
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.120"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            28..447
                     /locus_tag="PXD81_001730"
     CDS             28..447
                     /locus_tag="PXD81_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005704641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS sensor domain-containing protein"
                     /protein_id="HDL5537737.1"
                     /translation="MVPSNEAVLKAVHDTLVISTTDLQGVITYANDLFCKLTGFTREE
                     LIGQPHNIVRHPAVPKEVYKDMWDTIQAGKIWTGIVPNCGKGGVVYVVDTTVQPMFDE
                     TGKITGYISVRRVINDLMTNFEAVESAKEIFDDFYGE"
     gene            518..2074
                     /locus_tag="PXD81_001731"
     CDS             518..2074
                     /locus_tag="PXD81_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite reductase flavoprotein subunit alpha"
                     /protein_id="HDL5537738.1"
                     /translation="MSLIPHIHIAYGSESGNAEKLAQQLAIQPFLLPYSPTLSTLNET
                     DLSQLSAESLLLIVTSSFGDGEPPENADEFAEKLENLTACNLQYAIFGLGDITYDKFC
                     GYSKQLDSLLQQKQAKAVIARVDADLNYQEIFKQWLPLVEQALHSTNKETIQHSLSVQ
                     AYGEEMAYQAEVLEIKHLANSSPAVYHIRLSLKESGIFYQAGDLIYVKVQQPDRLLNE
                     YAEWFNDQNAVALLRHKELRLLSKNVLRDIQKICGSNELKDLTKISNKKALEQYVYGR
                     DLLDVLRDFDPNKSVTLADLEPILSNLAARAYSISSCGKTHPDYVDLCIRHVSYHLNG
                     RDYQGTASGYLSELQAGDFVSIFANANPNFRLPETLTAPVVMIGSGTGIAPHIAFLQA
                     LEQQYQGVESYLFFGERYRDKDFLYQTELENYLAKGTLTQLFTAFSRDQAEKFYVQDA
                     LSQQAELVWHLIQQGAYFYICGSKAMSKAIDAELIKIADQIGGDPYIDDFNNIIAKLV
                     AEGRLMRDVY"
     gene            complement(2132..2389)
                     /gene="rpsQ"
                     /locus_tag="PXD81_001732"
     CDS             complement(2132..2389)
                     /gene="rpsQ"
                     /locus_tag="PXD81_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005759643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HDL5537739.1"
                     /translation="MTDKIRTVQGKVVSDKMDKSFVVAIERTVKHPLYGKFIRRTTKL
                     HVHDENNEAKLGDVVEIKECRPISKTKSWTLVRVVEKAVVA"
     gene            complement(2389..2580)
                     /gene="rpmC"
                     /locus_tag="PXD81_001733"
     CDS             complement(2389..2580)
                     /gene="rpmC"
                     /locus_tag="PXD81_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HDL5537740.1"
                     /translation="MKAQELRNKSVEELNGELVNLLGEQFKLRMQAATGQLQQTHQLK
                     QVRRSIAQVKTVLTEKAGE"
     gene            complement(2580..2990)
                     /gene="rplP"
                     /locus_tag="PXD81_001734"
     CDS             complement(2580..2990)
                     /gene="rplP"
                     /locus_tag="PXD81_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HDL5537741.1"
                     /translation="MLQPKRTKFRKVHKGRNRGIAGGTEVSFGSFGLKAIGRGRLTAR
                     QIEAARRAMSRAVKRQGKIWIRVFPDKPITEKPLEVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVSEEVARNAFALAAAKLPFKTTFVTKTVM"
     gene            complement(3004..3711)
                     /gene="rpsC"
                     /locus_tag="PXD81_001735"
     CDS             complement(3004..3711)
                     /gene="rpsC"
                     /locus_tag="PXD81_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HDL5537742.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTQDFADNLDGDFKVRKFLN
                     KELANASVSRITIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRNAVAKIAGVPAQIN
                     IAEVKKPELDAKLVADSIASQLERRVMFRRAMKKAVQNAMKLGAKGIKVEVSGRLGGA
                     EIARSEWYREGRVPLHTLRADIDYSTAEAHTTYGVIGVKVWIFKGEILGGMAAVIESE
                     KEPAAQPKKAPRGKGRK"
     gene            complement(3731..4063)
                     /gene="rplV"
                     /locus_tag="PXD81_001736"
     CDS             complement(3731..4063)
                     /gene="rplV"
                     /locus_tag="PXD81_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HDL5537743.1"
                     /translation="METIAKHRYARTSAQKARLVADLIRGKKVSVALEILTFTNKKAA
                     TLVKKVLESAIANAEHNDGADVDDLKVAKIFVDEGPSMKRVMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            complement(4074..4349)
                     /gene="rpsS"
                     /locus_tag="PXD81_001737"
     CDS             complement(4074..4349)
                     /gene="rpsS"
                     /locus_tag="PXD81_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HDL5537744.1"
                     /translation="MPRSLKKGPFLDLHLLKKVEKAVESGDKKPIKTWSRRSMIIPSM
                     IGLTIAVHNGRQHVPVYVSDEMIGHKLGEFAPTRTYRGHAADKKAKK"
     gene            complement(4380..5201)
                     /gene="rplB"
                     /locus_tag="PXD81_001738"
     CDS             complement(4380..5201)
                     /gene="rplB"
                     /locus_tag="PXD81_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HDL5537745.1"
                     /translation="MAIVKCKPTSAGRRHVVKVVNAELHKGKPFAALLDTKSKTGGRN
                     NLGRITTRHIGGGHKQHYRLIDFKRNKLDIPGVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLTAGDQIQAGASAPIKVGNALPMRNIPVGTTVHNVELKPGKGGQIARSAGA
                     YVQIIAREGNYVTLRLRSGEMRKVLAECTATIGEVGNSEHMLRVLGKAGANRWRGIRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRHNKRTDKFIVRRRGK"
     gene            complement(5220..5582)
                     /locus_tag="PXD81_001739"
     CDS             complement(5220..5582)
                     /locus_tag="PXD81_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005818317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HDL5537746.1"
                     /translation="MIQQERLLNVLKAPHISEKATNNAEKGNTIVFKVALDANKVEIA
                     NAVEELFEVKVDSVRTVVVKGKTKRRGAKVDSVRTVVVKGKTKRRGAKVGRRSDWKKA
                     YVTLQEGQSLDFVEGAAE"
     gene            complement(5579..6181)
                     /gene="rplD"
                     /locus_tag="PXD81_001740"
     CDS             complement(5579..6181)
                     /gene="rplD"
                     /locus_tag="PXD81_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HDL5537747.1"
                     /translation="MELQVVGANALTVSETTFGREFNEALIHQVVVAYAAGARQGSRA
                     QKTRAEVSGSGKKPWRQKGTGRARSGDIKSPIWRSGSVTFAAKPQDHSQKVNKRMYRG
                     AIKSILSELVRQDRLVVVEKFEIDAPKTKVLAQKLKDMALNDALIITANVDENLFLAA
                     RNLYKVDVRDVQGIDPVSLIAFDKVVVTVDAVKQIEEMLA"
     gene            complement(6197..6826)
                     /gene="rplC"
                     /locus_tag="PXD81_001741"
     CDS             complement(6197..6826)
                     /gene="rplC"
                     /locus_tag="PXD81_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HDL5537748.1"
                     /translation="MIGLIGRKVGMTRVFTEDGVSIPVTVIEIEANRVTQVKTLENDG
                     YSAIQVTTGSKKASRVTKPEAGHFVKAGVEAGRGLWEFRTTEGEEFTLGQEINVDIFT
                     DVKKVDVTGTSKGKGFQGGVKRWNFRTQDATHGNSLSHRVLGSIGQNQTPGRVFKGKK
                     MAGHLGAERVTVQSLEVVRVDVERKLLLVKGAVPGATNGDVIVKPAVKA"
     gene            complement(6852..7163)
                     /gene="rpsJ"
                     /locus_tag="PXD81_001742"
     CDS             complement(6852..7163)
                     /gene="rpsJ"
                     /locus_tag="PXD81_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HDL5537749.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQSTAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHKRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
BASE COUNT         2131 a         1564 c         1365 g         2268 t
ORIGIN      
        1 taatggagat tcaacacggc tgaacaaatt gttccttcaa atgaagctgt tttaaaagca
       61 gtccacgata ctttagttat ttcaacaact gatttacaag gtgttattac ttacgctaac
      121 gaccttttct gcaaattaac cggttttacc cgtgaagaat taattggaca accgcataat
      181 atcgtacgcc acccagctgt accaaaagaa gtttataaag atatgtggga tacaatccaa
      241 gccggtaaaa tctggacagg tatcgtacca aactgcggta aaggcggtgt agtttatgtg
      301 gttgatacca ccgttcaacc aatgtttgat gaaacaggca aaattacagg ctatatcagc
      361 gttcgccgtg taattaatga tttaatgacc aactttgaag cagtagaatc agcaaaagaa
      421 atctttgatg acttctacgg tgaataatta aaaaacaatg attgcgtgta aaagatgatc
      481 tcttttacac gcaatttttt attttcgagg agtttgtatg tctctcattc cccatatcca
      541 cattgcttat ggttcggagt ctggtaacgc agaaaagctt gcccaacaat tagctatcca
      601 acccttttta cttccctact cacccacact ttctacatta aacgaaaccg atttaagcca
      661 attatctgcc gagagtttat tgcttattgt aaccagctcc tttggtgatg gcgaaccacc
      721 tgaaaacgcc gatgaatttg cagaaaaatt ggaaaatttg accgcttgta atctgcaata
      781 tgcgatattc gggctaggtg atattactta tgataaattc tgtggctata gcaaacaact
      841 agattcatta ttacaacaaa aacaagctaa agctgtgatt gcaagggttg atgccgattt
      901 aaactatcaa gaaattttta aacaatggct acccctagtg gagcaggcat tacattcaac
      961 aaacaaagaa acgatccaac attccctttc tgttcaagct tacggcgagg aaatggcata
     1021 ccaagcagaa gtgttggaaa ttaagcattt ggcaaactca agccctgcgg tgtatcacat
     1081 tcgtttatca ttaaaagaga gcggtatttt ctatcaagca ggggatttaa tttatgtcaa
     1141 agtgcagcaa ccagacagac tattaaatga atatgcagaa tggtttaacg atcaaaatgc
     1201 agttgcatta ctacgccata aagaactccg actactaagt aaaaatgtac tgcgtgatat
     1261 tcaaaaaatt tgcggcagta atgaactcaa agatttaact aaaatttcca ataaaaaagc
     1321 attggaacaa tatgtctatg ggcgggattt attagacgta ttacgcgatt ttgacccaaa
     1381 caaaagcgtg acattagccg atttagagcc aattttatct aacttagccg cacgagccta
     1441 ttcaattagc tcttgcggta aaactcaccc tgattatgtt gatttatgta tccgccacgt
     1501 ttcgtatcat ttaaacggta gagattatca agggactgca agtgggtatt tatccgaatt
     1561 acaagcgggc gattttgtct cgatttttgc aaatgccaat ccgaatttcc gtttacccga
     1621 aacattaacc gctccggtgg tgatgattgg ctcaggtact ggcattgctc cgcatatcgc
     1681 atttttacaa gcgttagagc agcaatatca gggggttgaa agctatttat tcttcggcga
     1741 gcgttatcgt gataaagatt ttctgtatca aacggaactg gaaaattact tagcaaaagg
     1801 caccttaact cagctgttta ctgctttttc acgtgatcag gcagagaagt tttatgtgca
     1861 agatgcccta tcgcaacaag cagagttagt ttggcattta attcagcaag gggcttattt
     1921 ctatatttgt ggtagcaaag caatgagtaa agcaatagat gcggaactca tcaaaattgc
     1981 agatcaaatt ggaggcgatc cttatattga tgatttcaat aatattattg caaaattggt
     2041 cgcagaaggc cgattaatgc gagatgtata ttagcaaatg aaaaaaacta aaaacccagc
     2101 aaaagctggg tttttattta atcatagttc attaagcaac aactgctttc tcaactacac
     2161 gaactaaagt ccaagattta gtttttgaaa taggacgaca ttccttaatt tcaacaacat
     2221 cacctaattt agcttcattg ttttcatcgt gtacatgaag tttagtagta cgacggataa
     2281 acttaccgta taacggatgt ttaactgtac gttcgatagc aacaacaaat gatttatcca
     2341 ttttgtcgct aaccacttta ccttgtactg tacgaatttt atcagtcatt attcacccgc
     2401 cttttcggtt aatacagttt taacttgtgc aatactacga cgcacttgtt ttaactggtg
     2461 ggtttgttga agctgaccgg tggctgcttg catacgcaat ttgaattgtt cacctaaaag
     2521 gttcactaac tcaccattca gctcttcaac acttttatta cgtagttctt gagctttcat
     2581 tacatcaccg tcttagttac aaatgtggtt ttgaacggaa gtttagctgc cgctaatgca
     2641 aacgcattgc gtgcaacttc ttcagaaaca ccatccattt catagagaac tttacccggt
     2701 tggattaagg ctacccagta ctcaacgtta cctttacctt tacccatacg gacttctaat
     2761 ggtttttcag taattggttt gtctgggaac acacggatcc agattttacc ttgacgttta
     2821 accgcacggc tcatcgcacg acgagcagct tcaatttgac gagctgttaa acgaccacgg
     2881 ccaatagctt ttaaaccaaa tgaaccgaag ctcacttctg tgccacccgc gataccacgg
     2941 ttacggcctt tgtgtacttt acggaatttt gtgcgttttg gttgtaacat tcagtgattc
     3001 tccttattta cgacctttgc cacgtggcgc ctttttaggc tgtgccgctg gttctttttc
     3061 agattcaatc actgcagcca ttccaccaag aatttcacct ttgaagatcc acacttttac
     3121 gccgattacg ccgtaagtag tgtgagcttc agcagtacta taatcgatgt ctgcacgaag
     3181 cgtatgtaga ggtacacgac cttcacgata ccattctgag cgagcaattt ctgcaccacc
     3241 taaacgaccg ctaacttcaa ctttgatacc tttagcacct aatttcatcg cgttttgaac
     3301 tgcttttttc attgcacgac ggaacatcac acgacgttca agttgagaag cgatgctatc
     3361 tgcgactaat ttcgcatcta gctctggttt tttcacttca gcgatgttga tttgagctgg
     3421 aacgccagcg attttcgcaa ctgcgttgcg taatttttca acatcttcac cttttttacc
     3481 gataacgata cccgggcgag ctgtgtgaat agttacacga atgctttttg caggacgctc
     3541 aatagtgatg cgtgatacag aagcgttcgc taactctttg tttaaaaatt tacgtacttt
     3601 gaagtcgccg tctaagttat cagcgaaatc ttgtgtattc gcgaaccaag tagagttcca
     3661 aggtttaaca atacctaggc gaataccatt aggatgtact ttctgaccca tttgttattc
     3721 tcctacttaa ttaacgatct gacacaacca cagtgatgtg gcttgtacgt tttaaaatac
     3781 gatctgcacg accttttgca cgtggcatta cacgtttcat gcttggacct tcatcaacga
     3841 agattttagc aactttaaga tcatcgacat ctgcaccgtc attatgctct gcgtttgcaa
     3901 tagctgactc taatactttc ttaacaagcg tcgctgcttt tttgttagtg aaagttaaga
     3961 tttctaatgc tacagaaact tttttaccgc gaattaaatc agcaactaag cgagcttttt
     4021 gggcagaagt acgagcgtaa cgatgtttag caatagtttc catcttttac ctcttatttc
     4081 ttagctttct tatctgcggc gtgaccgcgg tatgtacgag tcggtgcaaa ttcacctaat
     4141 ttatgaccga tcatttcgtc ggaaacataa acaggaacgt gctgacgacc attatggact
     4201 gcgatggtca aaccaatcat tgatggaatg atcattgaac gacgggacca agttttaatt
     4261 ggttttttat ccccgctttc caccgccttc tctaccttct tcaacaagtg taggtcaagg
     4321 aaaggacctt tcttgaggga acgtggcatt ggtgtttacc ttttagttaa tgaattaaat
     4381 tatttgccac gacgacgtac gatgaattta tcagtacgtt tgttgtgacg agttttctta
     4441 cctttggttt gaacgcccca aggtgtaaca ggatgtttac cgaagttacg accttcacca
     4501 ccaccgtgtg ggtgatctac cgggttcatt gcagtaccac gaacagttgg acgaatacct
     4561 ctccaacggt ttgcacctgc tttaccaaga acgcgaagca tatgctctga gttacctact
     4621 tcaccaatgg ttgcagtaca ctcagctaat actttacgca tttcgcctga acgaagacgt
     4681 aaagttacat agttaccttc acgggcaatg atttgaacgt aagcaccagc agaacgtgca
     4741 atttgaccac ctttaccagg ttttaattca acgttgtgta cagttgtacc tactgggatg
     4801 ttacgcattg gtaatgcgtt acctacttta atcggagctg aagcacccgc ttgaatttga
     4861 tcaccagcag ttaaaccttt tggtgctaaa atatagcgac gttcaccatc tttataaagt
     4921 actaatgcga tattcgcaga acgatttgga tcgtattcta aacgctcaac aacacccgga
     4981 atgtctaatt tattacgttt aaaatcaatt aaacggtagt gttgtttgtg accgccacca
     5041 atgtgacgag ttgtgatacg acctaagttg ttacgaccac cagttttaga tttagtatct
     5101 aaaagtgctg caaaaggttt acctttgtga agctcagcgt ttacaacttt aacaacgtga
     5161 cgacgaccag cggaggtcgg cttacattta acgatagcca tttattttat ctcctctgat
     5221 tactctgccg caccttcaac gaagtcaagc gattgacctt cttgaagagt tacataagct
     5281 tttttccagt cactacgacg accaacttta gcaccacggc gtttagtttt acctttaact
     5341 actacggtac gaacagaatc aactttagca ccacggcgtt tagttttacc tttaactact
     5401 acggtacgaa cagaatcaac ttttacttca aaaagttctt caactgcatt tgcaatctca
     5461 actttgtttg catctaatgc aactttgaat acgattgtat tgcctttttc tgcgttgttt
     5521 gtcgcttttt cagagatatg tggtgcttta agcacgttta gcaaacgttc ttgttgaatc
     5581 atgctaacat ctcctcaatt tgttttactg catctacggt tacaaccact ttatcgaaag
     5641 caattaagct aactgggtca ataccttgca catcacgcac atcaacttta tataagttgc
     5701 gtgctgctaa gaataaattc tcatctacgt ttgcagtaat gataagagcg tcatttaacg
     5761 ccatatcttt taatttttgt gctaatactt tagtttttgg tgcatcgatt tcgaatttct
     5821 caacaaccac taaacggtct tgacgaacta attcagaaag aatgcttttg attgcaccac
     5881 ggtacattct tttgttcact ttttggctgt gatcttgtgg tttcgcagca aaagttacac
     5941 tacccgaacg ccaaattggt gatttaatat caccagaacg tgcacggcct gtaccttttt
     6001 gacgccaagg ttttttacct gaaccagaca cttcagcacg agttttttga gcacgagatc
     6061 cttgacgagc acctgctgca taagcaacaa ctacttggtg gattaatgct tcattgaact
     6121 cacgtccgaa ggtagtttca gaaacagtta gtgcgtttgc acctacaact tgtaattcca
     6181 tctctatctc ctagacttat gctttaactg ccggttttac gataacatca ccattagttg
     6241 caccaggaac ggcaccttta actaataata atttacgctc aacatctaca cgaacaactt
     6301 caagtgattg aacggttaca cgctctgcac ctaagtgtcc tgccattttt ttacctttaa
     6361 acacacgacc tggagtttgg ttttgaccaa tagaaccaag tacacgatgt gataaagagt
     6421 taccgtgagt tgcatcttga gtacggaagt tccaacgttt aacaccacct tggaagcctt
     6481 taccttttga tgtaccagta acatcgactt ttttaacatc agtaaagatg tcaacattga
     6541 tttcttgacc taaagtgaat tcttcacctt cagtagtacg aaattcccat aaaccgcgtc
     6601 cagcttcaac acctgctttc acgaaatgac ctgcttctgg tttagttaca cggctagctt
     6661 ttttagagcc agtagtaact tgaattgcag agtagccatc gttttcaagg gttttaactt
     6721 gagtaacacg gttggcttcg atttcgataa cggtaactgg aatagacacg ccgtcttcag
     6781 tgaaaacgcg ggtcatacca actttacgac cgattaaacc aatcattgta ataacctctt
     6841 aaaattccca attaacctag actaatctga acgtcaacgc cagcagctag atctaaacgc
     6901 attaatgcat caacagtttt ttctgttggt tcaacaatat caactaaacg tttgtgagta
     6961 cggatttcat attggtcacg tgcatcttta ttcacgtgtg gagaaatcaa tacagtgaaa
     7021 cgttctttac gagttggtaa aggaattgga ccacgaactt gtgcaccagt acgtttagct
     7081 gtttctacga tctccgcagt agattgatca attaaacgat gatcaaatgc ttttaagcgg
     7141 atacggattc tttggttctg cattagacca gagctccaat taaaaaattt agctaataaa
     7201 aaaaccaaac taccacttaa caaattagtt aagggagctc agttaaacct atatagttcc
     7261 aaaattagga acattgtata cactacaata tctgtagtgc catttgataa atgattacat
     7321 caaatgtc
//
LOCUS       DANLYI010000121         7314 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.121, whole genome shotgun sequence.
ACCESSION   DANLYI010000121 DANLYI010000000
VERSION     DANLYI010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 7314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7314
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.121"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            10..468
                     /gene="exbB"
                     /locus_tag="PXD81_001743"
     CDS             10..468
                     /gene="exbB"
                     /locus_tag="PXD81_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-system energizer ExbB"
                     /protein_id="HDL5537750.1"
                     /translation="MEQLFQFLEHHTDFVILGLLGLLSVICLWKAIERIMFYSKVDIS
                     KYDTLEELDIDLTHNLTTISTIGSNAPYIGLLGTVVGILLTFYHLGQSGGDIDAASIM
                     VNLSLALKATAVGILVAIIAMVFYNGFGRKVEENKLKWLALQSKKPHQAV"
     gene            505..942
                     /gene="exbD"
                     /locus_tag="PXD81_001744"
     CDS             505..942
                     /gene="exbD"
                     /locus_tag="PXD81_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HDL5537751.1"
                     /translation="MKKFDEINIIPFIDIMLVLLAIVLVTASFISQGKIQVNVPKAST
                     TQPMKADDLAKLLTITENNEFFFNDQAITKEQLIAEVATWDKSQKVSLKVDGAVAFEK
                     FVELTDILSANEIKNVAIITKKETAPAPSSTPGSPAQVPAVAP"
     gene            958..1869
                     /locus_tag="PXD81_001745"
     CDS             958..1869
                     /locus_tag="PXD81_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HDL5537752.1"
                     /translation="MKKKHSRIGLLASLAIHTALIGGTFAWLHNHKVEKIAEQNSLSM
                     EMVAALLEQPQVAVAPDPITEEVKEIEPEKAEVPPEPKLTPEPTPEPEAIPDPTLNPK
                     KEKPKEEKRKERHHHKHKHRHKEKREELQEQEKPKDKPKERPKHHHKHIKALERGPEI
                     KQGIVAKAIPNAAEGEKVVAGVVGGQKNGSPNSTSTTGSANTGASSGNASELAAYKVA
                     LQRALQRKANNSYPQREKMMRKTGTVTIKFTITSSGSITNVSVLNSSGNSNLDNAAVR
                     SAEGLSMPPPPAGFPPTVTVPVRFALE"
     gene            complement(1938..2819)
                     /gene="cysZ"
                     /locus_tag="PXD81_001746"
     CDS             complement(1938..2819)
                     /gene="cysZ"
                     /locus_tag="PXD81_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HDL5537753.1"
                     /translation="MSSVFSEPQNQVGLGFHYFIYGWRLLLQRQFLPFVIFPILINTV
                     LMVGLIWGFFANIGGLLDMLLPSWLEWLSFVLIPLIFLMILVGFYFAFTTLANFVAAP
                     FNALLAEKVELQLTGEPLGDMSFGAMLKDVPRMLKREWQKMMYSIPRLIALFVLGFLP
                     VVGQTVAPVLTFIFGAWLIAIQYCDYPFDNHKISFQRMRNALSQSRTLNFTFGALVSL
                     FTMIPFVNLVVMPVAVCGATALWVKEYRNFFLNNQTGEFSKADYTFTKVNSTSLSTET
                     RTGEVSPNVRNGDIRKQ"
     gene            2931..3833
                     /gene="zipA"
                     /locus_tag="PXD81_001747"
     CDS             2931..3833
                     /gene="zipA"
                     /locus_tag="PXD81_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HDL5537754.1"
                     /translation="MELHIIFFILAGLLIAVLIGFSLWSARREKSNIFSNTFSTRPIA
                     VPPQNNKMSNSIPTSLQPQNEDYQAMSVPEENPQQIQQEVESSLQNIKISLPGGNPQQ
                     QPEQIQPIQSVAEPTVSTYGQKISNQQHIQYNTPDFANPMVNTVVEETPQAVAIEPQE
                     TQSAEEENPFVTLYLYAPQNEAFRGDFVVHYLEEVGLRFGEHQIFHRHQHIDDSLSPI
                     IFSAANLMNPGTFDLNNISNFATAGLVLFMQLPTEGNDVANMKLLISNAEHLAASLGG
                     FLYDDNQQPFGEESRQRYMQRVMH"
     gene            3898..5955
                     /gene="ligA"
                     /locus_tag="PXD81_001748"
     CDS             3898..5955
                     /gene="ligA"
                     /locus_tag="PXD81_001748"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HDL5537755.1"
                     /translation="MSLFNQTSLFEENATPELVEKIETIRQTLRQYEYEYHVLDNPSV
                     PDAEYDRLMNELKNLEWQHPELISADSPTQRVGAKPLAGFAQVTHEIPMLSLDNAFSD
                     EDLDGFLRRMESYITASPSQLEFCCEPKLDGLAVSILYENGILTRAATRGDGTTGEEI
                     TANIRTIRNIPLKLNTTNPPARLEVRGEVFMPQSGFNELNEKALAKGEKTFANPRNAA
                     AGSLRQLDPKITRQRPLVLNAYGIGVYESDDELPATHFERLQWLKSLGIPVNNEIRLV
                     QGKAALLAYYAEIQAKRPTLGYDIDGTVLKVNDIALQEQLGFISRSSRWAIAYKFPAQ
                     EEMTILKEVDFQVGRTGAITPVAKLEPVFVAGVTVSNATLHNGDEIERLGIVIGDTVI
                     IRRAGDVIPQITGVMLERRPENAKKIEFPTACPICHSAVTRVEGEAVARCTGGLFCEA
                     QRKEALKHFVSRKAMDIDGVGEKLIEQLMARELIETPADLFKLDHTTLMRLERMGEKS
                     AQNALNSIEKAKNTTLPRFLFSLGIREVGEATALNLANHFGSLEAIRQATFEQLKEVQ
                     DVGEVVANRIVSFWQEPHNVEVVEDLIAQGVRWENVVKAEIVDNPLKDKNVVLTGTLT
                     QLTRDEAKALLQSLGCKVSGSVSSKTDYLIAGEKAGSKLAKAQELGIQILSEQQFIEL
                     TQH"
     gene            complement(6018..7286)
                     /locus_tag="PXD81_001749"
     CDS             complement(6018..7286)
                     /locus_tag="PXD81_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HDL5537756.1"
                     /translation="MTQQIEHSTWQSKFAAMGPGILMASAAVGGSHIIASTQSGAIYG
                     WQLAIIILLANLFKYPFFRFGVQYTLASGKTLLEGYKEKGKFYLIIFFILNVFAAMIN
                     TAAVAIVSAAILKFIFSALGIPLDLSIPMAGTIVIVVTWGILLLGQYKFLDTLSKWIM
                     IALTISTVSAVIIAALKGGVEIAPDFVEPSPWNLAALVFIVALMGWMPAPIEISAINS
                     MWVIAKKRLSKVNYEDGIFDFNVGYIGTAILAIVFLALGALVQYGSGEKVEQAGVAYI
                     AQLIKMYAFAIGDWSKLLIAFIAFMCMFGTTITVIDGYSRANAEALRLISGRKQHSQT
                     ELSIWMTVTALIGILIISQFMSDVAKMLQFAMISSFVSTPVFAWLNFSLVLKGEHRAK
                     GWLFWLAVIGLIYLTSFTLLFLAQQFKLIG"
BASE COUNT         2401 a         1585 c         1438 g         1890 t
ORIGIN      
        1 gaggatttta tggagcaatt atttcaattt ttagaacatc atactgactt tgttatatta
       61 ggattactag gcttattaag cgtaatctgc ctttggaaag ctattgaacg tattatgttt
      121 tattccaaag tagatatttc aaaatacgat accctcgaag aattagacat cgatttaacc
      181 cacaatttaa ctaccatttc gactatcggc tctaatgccc cttatatcgg cttattagga
      241 accgttgtcg gtattctttt aactttctat catttaggtc aatcaggtgg tgatattgat
      301 gcggcctcta ttatggtaaa cctttcttta gctctaaaag ccactgctgt cggtatctta
      361 gtggcaatta ttgcaatggt tttctacaat ggttttggtc gtaaagtgga agaaaacaaa
      421 ctgaaatggt tggcattaca atcaaaaaag cctcatcaag cggtgtgatt tagtccattt
      481 tttgcaaatt aataaggcag aacaatgaaa aaattcgatg aaatcaacat cattccattt
      541 attgatatta tgttggtatt actggctatc gtgttagtta ccgcctcttt catttctcag
      601 ggtaaaatcc aagtaaatgt accgaaagca agtacaacac agccaatgaa agcagacgat
      661 ttagcgaagc ttttaaccat taccgaaaat aatgagtttt tctttaatga tcaagcaata
      721 actaaagagc aattaattgc tgaagttgcc acttgggata aaagccaaaa agtgtcactc
      781 aaagttgatg gtgcagtcgc cttcgaaaaa tttgtggaat taaccgatat cctttctgca
      841 aatgaaatta aaaatgtagc aatcattact aaaaaagaaa ctgctccagc accttcaagc
      901 acacctggta gccctgcaca agtgccagca gtcgcacctt aaatagggta aacaggtatg
      961 aaaaaaaaac attctcgtat cggcttattg gcatctttgg ctattcacac tgccttaatt
     1021 ggtggcactt ttgcttggtt acataaccac aaagtggaaa aaattgccga gcaaaactct
     1081 ctttctatgg aaatggtggc agcattatta gagcaacctc aggttgccgt tgctccggat
     1141 cctattaccg aagaggtcaa agaaatcgaa ccggagaagg cagaagtacc acctgaacct
     1201 aagcttactc cggagccaac tccagagccg gaggcaattc cagatcctac gctaaatccg
     1261 aaaaaggaaa aaccgaaaga agaaaagcgt aaagagcgac accaccataa acataagcat
     1321 cgtcacaaag agaaaaggga agagctacaa gagcaagaaa aaccgaaaga taagcctaaa
     1381 gagcgcccga aacatcatca caagcatatt aaagcattag agcgcgggcc agagataaaa
     1441 caaggtattg tcgctaaagc gattccaaat gcggctgaag gtgaaaaagt ggttgcgggg
     1501 gttgttggag ggcaaaaaaa cggttcacca aattcaacct caactacagg ttcagcaaat
     1561 actggggcta gcagtggtaa tgccagtgaa cttgctgctt ataaagtcgc attacagcga
     1621 gcattacagc gtaaggcgaa taactcttac cctcagcgtg aaaaaatgat gcggaaaacc
     1681 ggtacagtta cgattaaatt taccattaca tcgtccggtt caattacaaa tgtatccgta
     1741 ctcaattcat cgggaaatag taatttagat aacgctgcgg tacgcagtgc agaaggtttg
     1801 agtatgcctc cgccaccggc aggcttccct cctacggtaa cagtaccggt aagatttgcc
     1861 cttgaataaa aacagccctc aaaagcaatg tacttttgag ggctttttat tgcaaaaaat
     1921 tgactaaaaa tgaccgctta ctgcttgcga atatcgccat ttctcacatt cggactcact
     1981 tcacccgtac gagtttctgt gcttaacgag gtagaattta ctttcgtaaa agtataatcc
     2041 gctttgctga attcacctgt ttgattattt aagaagaaat tgcggtactc ttttacccat
     2101 aatgccgttg cgccacaaac tgccacaggc ataactacta aattcacaaa tggaatcatc
     2161 gtgaataggc tcactaaagc accaaaggta aaatttaaag tgcgagactg cgacaacgca
     2221 tttcgcatac gctgaaaact gattttatgg ttatcaaacg gatagtcaca atattgaatg
     2281 gcgatcaacc aagcaccgaa gataaaagtc agcacaggtg ctacggtttg cccaaccaca
     2341 ggcaaaaagc ctaatacaaa aagagcaatc agccttggaa tgctatacat cattttttgc
     2401 cattctcgtt tcagcattcg aggcacatct tttaacattg caccgaatga catatcgccc
     2461 aaaggctcgc cggtaagttg cagttcgact ttttctgcta ataacgcatt aaagggagca
     2521 gcaacaaagt tagccaaggt agtaaaagca aaataaaatc ccactaatat catcaaaaaa
     2581 atcagtggaa tcagtacaaa gctaagccat tccagccagc ttggcagtaa catatccaac
     2641 aagccaccga tattggcaaa aaatccccaa atcagcccta ccatcagcac ggtattaata
     2701 agaattggga aaatcacaaa aggcaaaaat tgacgttgta acaataaccg ccagccatat
     2761 ataaaataat gaaagcctaa tccgacttga ttttgcggct cagaaaaaac tgaagacata
     2821 atgattcctt attttctatc aaaattaatt agaaatatgg taagctacgc acattatttt
     2881 cgcaaatatg aaaatacatt ttttattgat tatttgataa agaggacaca atggaactgc
     2941 atattatttt ttttattctt gccgggctac tgattgctgt actaattgga tttagccttt
     3001 ggtccgctcg tcgtgaaaaa tcaaatattt tttcaaatac attctcgacc agacctattg
     3061 ctgtgccacc acagaataat aaaatgagca atagcatccc aacctcatta cagccacaaa
     3121 atgaggatta tcaagcaatg tctgtgccgg aagaaaatcc gcaacagata caacaggaag
     3181 ttgaaagctc gctgcaaaat atcaaaatca gtttgccggg cggaaatcct cagcaacagc
     3241 ctgagcaaat tcagccgata caatcggtag cagaaccaac ggttagcact tacggtcaga
     3301 aaatttctaa tcagcaacat atccaataca atacgccgga ttttgcaaac ccaatggtga
     3361 acacagtggt ggaagaaaca cctcaagcag tagctatcga acctcaagaa acccaatcgg
     3421 cagaggaaga aaatccattt gtaaccctct atctttacgc accacaaaat gaagctttcc
     3481 gcggtgattt tgtagtacat tatttagaag aagttggctt acgttttggc gaacaccaaa
     3541 ttttccaccg ccatcaacat attgatgatt cattaagtcc gattattttc agtgcagcaa
     3601 atttaatgaa cccaggaacc tttgatttaa ataatatctc aaactttgcg acagctggtt
     3661 tagtgctatt tatgcaactt ccaaccgaag gcaatgatgt ggctaatatg aagttgttaa
     3721 tcagtaacgc tgaacactta gcagcatcgt tagggggatt cttatatgac gataatcaac
     3781 agccttttgg cgaagaatcc cgccaacgtt atatgcaacg tgtgatgcac taattgcgaa
     3841 aaaattaata aatccgaccg cttgtacaag cggtcttttt tgtagagaat tttaccaatg
     3901 tctttattta accaaacctc tttatttgaa gagaatgcca cgcccgagtt agtcgagaaa
     3961 atcgaaacta ttcgccaaac attgcgtcaa tacgagtatg aatatcacgt tttagataac
     4021 ccaagcgtac ccgatgccga atacgaccgt ttaatgaacg agctgaaaaa tctggaatgg
     4081 cagcacccgg aactgattag tgccgactcg ccgactcaac gggttggtgc caaaccgctg
     4141 gcaggctttg ctcaagtcac ccacgaaatc cctatgcttt cgttggataa tgccttctcc
     4201 gatgaagact tagatggctt tttacgccgt atggaaagct atatcaccgc ctctccttcg
     4261 caactggaat tttgttgcga acctaaatta gacggcttgg cagtgagtat tttatacgaa
     4321 aacggtatac ttacccgagc tgcaacccgt ggcgatggca caaccggcga agaaattacc
     4381 gctaatatcc gcaccattcg caatattccg ttaaagctca acacgactaa tccacctgct
     4441 cgattagagg tgcgaggcga agtctttatg ccacaatcag gctttaatga gctgaatgag
     4501 aaagccttag ctaaaggcga aaaaaccttt gccaaccctc gcaatgccgc agcaggttcg
     4561 cttcgccagt tagatccgaa aatcacccgc cagcgtccgt tagtgctgaa tgcctacgga
     4621 attggggtgt atgaaagcga tgacgaactg ccagccaccc atttcgaacg tttgcaatgg
     4681 ctgaaatcgc ttggtattcc tgtaaataat gaaatccgct tggttcaagg caaagctgct
     4741 ctcttggctt attatgcgga gattcaggct aaacgcccaa cattaggcta tgatattgat
     4801 ggcacagtat tgaaagtaaa tgacattgcc ctacaagagc aactcgggtt tatctctcgc
     4861 tcctctcgtt gggcgattgc gtataaattt ccggctcaag aagaaatgac aatcctcaaa
     4921 gaagtggact tccaagtggg aagaacaggg gcaatcaccc ctgtggcgaa attagagcct
     4981 gtttttgtcg ctggcgtaac ggtaagtaac gcaacgctac acaacggtga tgaaatcgag
     5041 cgacttggta ttgtgattgg cgatacggta attattcgcc gagcgggcga tgtaattccg
     5101 caaattacag gcgtgatgtt agaacgccgc ccggaaaatg caaaaaaaat tgaatttccg
     5161 accgcttgtc ctatctgtca ctccgctgtt actcgggtag aaggcgaggc agtagcacgc
     5221 tgtaccggtg gtttattctg cgaagctcaa cgtaaagaag ccttgaaaca tttcgtttca
     5281 cgcaaggcaa tggatattga cggcgtaggc gaaaaactaa tcgagcagtt aatggctcga
     5341 gagctgattg aaacgcctgc cgatctgttt aagctcgatc acactaccct aatgcgatta
     5401 gagcgaatgg gggaaaaatc ggctcaaaat gctctcaaca gcatcgaaaa agcaaaaaac
     5461 actactcttc ctcgcttttt attctcgtta ggtattcgtg aagtgggcga ggcaaccgct
     5521 ctcaacttag ccaaccattt tggtagctta gaggcgatcc gccaagccac ttttgagcaa
     5581 ttaaaagaag tgcaagatgt gggcgaggtt gttgccaacc gtattgtcag cttctggcaa
     5641 gaaccgcata atgtggaagt ggtggaagat ttgatcgctc aaggtgtacg ttgggaaaac
     5701 gttgtgaaag ccgaaattgt agataacccg ttaaaagata aaaacgtggt cttaaccggc
     5761 acgctaaccc agctcacccg tgatgaagcc aaagccctat tacaaagttt gggctgtaaa
     5821 gtctccggct cggtttccag caaaaccgat tacttaatcg ccggcgaaaa agccggttcc
     5881 aagctggcaa aagctcaaga gttgggaatt cagattttaa gcgagcaaca atttattgag
     5941 cttactcaac attaacaaac aaaggggcag atgattgaaa tctgcccctt attctatcta
     6001 ttgaatgctt aaaagcatta accaatcaat ttaaactgct gagctaaaaa cagtaacgta
     6061 aagctagtta agtaaattaa gccgataact gccaaccaaa ataaccagcc ttttgcacga
     6121 tgctcacctt ttagcactaa agagaaattt aaccacgcaa aaacaggggt agaaacaaat
     6181 gatgaaatca ttgcaaattg taacattttc gccacatcac tcataaattg gctaataatt
     6241 aaaatgccga tgagtgcagt taccgtcatc caaatactta attctgtttg tgaatgttgc
     6301 ttacgcccgc taattaaacg caacgcttct gcattcgctc gtgaatatcc atcaataaca
     6361 gtgatcgttg tgccgaacat acacataaat gcaataaatg cgattaaaag cttagaccag
     6421 tcaccaatcg caaaagcata cattttgatt aattgagcaa tataggcaac accggcttgt
     6481 tctactttct cacctgaacc gtactgaact aacgcaccta atgctaaaaa aacaatcgcc
     6541 aaaattgccg tgccgatata acccacatta aaatcaaaaa taccgtcctc ataattcact
     6601 ttacttaaac gttttttcgc aataacccac attgagttaa tggcagaaat ttcaattggt
     6661 gcaggcatcc agcccattaa tgcaacgatg aacacaagtg ctgctaaatt ccacggcgaa
     6721 ggctctacga aatctggagc aatttcaacg ccacctttta aagcagcaat gatcacggca
     6781 gataccgtag agatggttaa agcaatcata atccatttag ataatgtatc taagaattta
     6841 tattgcccta gaagtaaaat accccaagtg accacaatca caatagttcc tgccattgga
     6901 atgcttaaat ctaacggaat acctaaagca gaaaaaataa attttagaat cgctgctgaa
     6961 acaatcgcca ccgcagctgt gttaatcatt gcagcaaaaa cgttaaggat aaagaaaata
     7021 ataagataaa actttccttt ttctttgtag ccttccaata aagttttacc gctagctaag
     7081 gtatattgca caccaaagcg gaaaaacgga tatttaaata aattggctaa cagaataata
     7141 atcgccagct gccagccata aatcgcacca gattgtgtag aggcaataat atgcgaacca
     7201 cccactgccg cagatgccat caaaatcccc ggccccattg cagcaaattt actttgccaa
     7261 gttgaatgtt ctatttgttg tgtcatatag ctttccttat cacattacta tcaa
//
LOCUS       DANLYI010000122         7268 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.122, whole genome shotgun sequence.
ACCESSION   DANLYI010000122 DANLYI010000000
VERSION     DANLYI010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 7268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7268
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.122"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            27..1013
                     /gene="cas1c"
                     /locus_tag="PXD81_001750"
     CDS             27..1013
                     /gene="cas1c"
                     /locus_tag="PXD81_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014167737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated endonuclease Cas1"
                     /protein_id="HDL5537757.1"
                     /translation="MTTQGSYLHKERETLVVEQDRKKVAQLPIHSIGHIFCFGNVLVS
                     PFLMGFCGENNVNLAFFTENGRYLGRLQGRQSGNVLLRRAQYKKSETNPVPVARNIIA
                     AKIQASKRVLQRQIRNHGENADLQSTITALNYSLQQLKTADNLDLIRGIEGDAAARYF
                     GVFSHLIKENSGFHFDGRNRRPPRDGVNALLSFLYSIVGKDISGALQGVGLDPQIGFL
                     HADRSGRDSLAQDILEEFRAWWVDRMVLSLINRGQVKPDDFVTEASGAVTLKPEARKL
                     LFQTLQAKKQEKIIHPFLEEEVEIGLLPYIQAMLLARHLRGDLAEYPPFLMR"
     gene            1080..1373
                     /gene="cas2"
                     /locus_tag="PXD81_001751"
     CDS             1080..1373
                     /gene="cas2"
                     /locus_tag="PXD81_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005540845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HDL5537758.1"
                     /translation="MLMLITYDISFDDPEGQARLRRIAKHCLDYGVRAQYSVFECDVT
                     PAQWVTLKNKLLATYNPDCDSLRFYHLGSKWRNKVEHHGAKPSVDIFKDTLIL"
     repeat_region   1565..2600
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=1565..1584
                     /rpt_unit_seq="cagccacctacgcgtggctg"
     gene            2850..4325
                     /gene="rng"
                     /locus_tag="PXD81_001752"
     CDS             2850..4325
                     /gene="rng"
                     /locus_tag="PXD81_001752"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005610087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HDL5537759.1"
                     /translation="MSSTELLVNVTPSETRVALVENGILKELHIEREAKRGIVGNIYK
                     GRVTRVLPGMQSAFVDIGLEKAAFLHASDIVSHTECVDENEKKQFLVKDISELVREGQ
                     DIVVQVVKDPIGTKGARLTTDITLPSRYLVFMPENSHVGVSQRIESEEERARLKSLVE
                     PYCDELGGFIVRTAAEEATEESLKQDVEFLKRLWRKVLERKGKYSAKSMLYGELALAQ
                     RVLRDFIGTHINSVRIDSKLTYEQVREFLAEFMPELTDCVTLYTGQQTLFDAYGVEKA
                     IQKALDKRVDLKSGGYLIIEQTEAMTTIDINTGAFVGHRNLAHTIFNTNIEAIQAIAH
                     QLQLRNLGGIIIIDFIDMQEEEHRERVLQSLQEALKGDRVKTNVNGFTQLGLVEMTRK
                     RTRESLERILCCDCQACQGRGTVKTVETVCYEIMREIVRVHHLFKTENIHVYAAKDVA
                     EYLINEESHALLAELQAFIGKKIEIKLEPYYHQEQFDVVVM"
     gene            4479..5981
                     /gene="lysS"
                     /locus_tag="PXD81_001753"
     CDS             4479..5981
                     /gene="lysS"
                     /locus_tag="PXD81_001753"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012054447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HDL5537760.1"
                     /translation="MSEVENQELDLNGEMLARREKLNKIREQGNAFPNTFRRDALAKD
                     LHAQYDAVEGETLKEQNIQVKVAGRIMLKRVMGKASFFTIQDVSGAIQLYVARDNLSD
                     SVYEEKVSLWDLGDIVGVEGALFKTKTGELTVRCSEVQLLTKSLRPLPDKHHGLTDLE
                     TRYRQRYLDLISNEESRRTFMIRSQVVSGIRKFFLERDFIEVETPMLQVIPGGAAAKP
                     FITHHNALDVDMYLRIAPELYLKRLVVGGFERVFELNRNFRNEGVSVRHNPEFTMIEY
                     YQAYADYHDLMDNTEELLRQLALDILGTTDVPYGDYVFDFGKPFERITMHDAIVKYGN
                     GIQREDLDSFEKSVEIAKGLGIEIQKSWGLGSVVNAIFEEVAEHQLIQPTFLMAHPAE
                     ISPLARRNDENPEVTDRFELFIGGREIGNGFSELNDAEDQAERFDAQVAAKDAGDDEA
                     MFKDEDFIVALEHGLPPTAGEGLGIDRLVMIFANAPSIRDVILFPAMRQK"
     gene            complement(6059..7132)
                     /locus_tag="PXD81_001754"
     CDS             complement(6059..7132)
                     /locus_tag="PXD81_001754"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HDL5537761.1"
                     /translation="MAQQITLFEDVPLPLTLGGELSPITVAYQTYGRLNAEKSNAVLL
                     CHALTGDAKPYYDSPTEKGWWQDFIGKGLAFDTDKYFFICSNVLGGCKGTTGPASINP
                     KTNKPYGSLFPIITVQDIVNVQKALLDYLEIPQLHAVVGGSFGGMQATQWGISYPDKV
                     RNIINLCSSLTLSAEAIGFNHVMRQAIINDPHFNGGNYYDGIAPDNGLKIARMLGMLT
                     YRTDIQLAKAFGRETKQQGQIWGDHFQVESYLSYQGTKFLGRFDANTYLRLLRALDLY
                     DPALGYENEETALKRIKANYTFVAVTSDQLFKQVDVYKSKQKLEQAGVNLEFHEFHSD
                     FGHDAFLVDYDFFEPMIKAGLAK"
BASE COUNT         2118 a         1355 c         1672 g         2123 t
ORIGIN      
        1 tgcgcaaact acaaaacacc ctctacatta ccacccaagg tagctatttg cacaaagagc
       61 gagaaacctt ggtggtggag caagatcgga agaaagtggc acagttgccg attcattcca
      121 ttgggcatat tttttgtttt ggcaatgtgt tagtgtcgcc gtttttaatg ggattttgtg
      181 gcgaaaataa cgtaaattta gcgtttttta ccgaaaatgg gcgttatctt gggcggttgc
      241 aggggcggca aagtggtaat gtattgttac gccgagccca gtataaaaag tcggagacca
      301 accctgttcc tgtggctcgc aacatcattg cagccaaaat tcaagcatct aagcgggtgt
      361 tacaacggca aattcgtaat cacggcgaaa atgctgacct tcaatcgaca attaccgcat
      421 taaattacag cctgcaacag ttgaaaacag cagacaattt ggatttgatt cgtggcattg
      481 agggcgatgc tgcggctcgt tattttgggg tgttttctca tcttatcaaa gaaaatagcg
      541 gtttccactt tgacggacgt aatcgccgcc cacctcgtga cggtgtgaat gcgttgcttt
      601 catttcttta tagcattgta gggaaagata ttagcggggc attgcaaggc gtggggcttg
      661 atccccagat tggctttttg catgcagatc gttcggggcg agatagctta gctcaagata
      721 ttttggagga atttcgagcg tggtgggtgg acagaatggt gctgtcgctg atcaatcgag
      781 ggcaggttaa gcctgatgat tttgtgacgg aagccagtgg tgcggtaacg ctcaagcctg
      841 aagccagaaa attactattt caaaccttgc aagcgaaaaa acaagaaaaa atcatccacc
      901 catttttaga ggaagaagtg gaaatcggct tgttacctta tatccaagcg atgttattgg
      961 ctcgtcattt gcgtggcgat ttggcagaat atccgccgtt tttaatgcgt taaaatctgc
     1021 aaaattttgt tgaaatttga ccgcttgttt atgttttatt cattgaggaa ttagcaacta
     1081 tgctgatgtt gattacctac gacatttcat ttgatgaccc cgaagggcag gctcgcctac
     1141 gccgtattgc taagcattgt ttggattatg gtgtgcgggc gcaatattct gtgtttgagt
     1201 gtgatgttac tcctgctcag tgggtaacat tgaaaaataa attattggca acttacaacc
     1261 cagactgtga tagtttgcgc ttttaccatt taggcagtaa atggaggaat aaagttgagc
     1321 accatggcgc aaaaccttca gtggatattt ttaaagatac gctgatttta taaatcgcta
     1381 gcctgtagtt ctcataaaaa tgtagcaagg ttagcgaatc tatattgttc tttaataatc
     1441 aaggaattag aaaaaataag gcacaatata ttggcgatat gctatacttt atccactctc
     1501 tacaaaacgt tagcgaataa agggctaaaa agcctgatat ttcaatacgt tatcttatag
     1561 ggaccagcca cctacgcgtg gctgcgaatt gaaaccctat aacgagcaat ggcattttga
     1621 tctacggtaa accagccacc tacgcgtggc tgcgaattga aacttcttga atacctccgg
     1681 agtcagttaa gataaggcca gccacctacg cgtggctgcg aattgaaacc accgctgcta
     1741 cttcggttat cggagtagtt tgcacccagc cacctacgcg tggctgcgaa ttgaaacgtg
     1801 gcacagtaac acgagcagat gctgataaac gcccagccac ctacgcgtgg ctgcgaattg
     1861 aaaccagcag ccgttgttac cgtggtgcaa tgcttgcgcc agccacctac gcgtggctgc
     1921 gaattgaaac tacacttatt gccatagggt agtagtaatc ttttacccag ccacctacgc
     1981 gtggctgcga attgaaactg cgatttttgg atttttcggg cgataaatga taccagccac
     2041 ctacgcgtgg ctgtgaattg aaactcgtgg ctggttatac aaagaaaagc attgttgggc
     2101 agccacctac gcgtggctgt gaattgaaac ttaaatctaa agctaaagct attttattaa
     2161 ttaaagcagc cacctacgcg tggctgtgaa ttgaaacttt accgtttacc gattggattt
     2221 taatgctaga ggcagccacc tacgcgtggc tgtgaattga aaccctaaat tgccgtaaaa
     2281 atctgccatt agctacgcgc agccacctac gcgtggctgt gaattgaaac ctcataacga
     2341 atccaatcct gttcggttaa tccccgcagc cacctacgcg tggctgtgaa ttgaaacagg
     2401 ggcgtgtacc gcaatcaatt ttaattataa ccaagcagcc acctacgcgt ggctgtgaat
     2461 tgaaacttta tctgttgcgg tcaattgtat atcttgaatt tgcagccacc tacgcgtggc
     2521 tgtgaattga aacctcgtct tgaattggaa aatcaagggc gacaataggc agccaccttc
     2581 gcgtggctgt gtattgaaac ggtgtaccca caaatcctat gttttcgcca ttggctgcta
     2641 ccttcgcatc aatatatatt gaaatatcaa tggcaaattt gtatgttgtt aattagactg
     2701 attgcatctg ctatgtatca tagtaatgac gaaaatattc tattttgcaa aaaataatca
     2761 gaaattaccc gcttgttcga actgagtttt tgttttctaa gatgtgaaat gatacacttt
     2821 agatagtttt aaaatataga gacagtaaga tgagtagtac ggaattatta gtgaatgtta
     2881 ccccaagcga aacaagggta gcgttggtag aaaacggaat attgaaagaa ttacatattg
     2941 agcgggaagc taaacgcggg attgttggta atatttataa gggaagggtg acaagggtat
     3001 taccaggtat gcaatctgct tttgtggata tcggcttgga gaaggctgct tttttgcacg
     3061 cttctgacat cgtttcgcat acggaatgtg ttgatgaaaa tgaaaagaag caatttctgg
     3121 ttaaagatat ttctgaattg gtgcgtgaag ggcaagatat agttgtacaa gtagtgaaag
     3181 atccaattgg tactaaagga gctaggctca ctactgatat tacgttacca tctcgttatt
     3241 tagtgtttat gccagaaaat agccatgtgg gagtatctca gcggattgaa agtgaagaag
     3301 agcgtgctcg tttaaaaagc ttggttgagc cttattgtga tgaacttggg ggctttattg
     3361 tacgtaccgc tgcagaagag gcaactgaag aaagtttaaa gcaagatgtt gaatttttaa
     3421 aacggttgtg gcgtaaagta ttagaacgaa aagggaaata ttccgcaaaa tcaatgcttt
     3481 atggagagct tgcgcttgct caaagggtac tgcgtgattt tattggtaca catatcaatt
     3541 cggttagaat cgattcaaag ctaacttatg agcaagtgcg agaattttta gctgagttta
     3601 tgccagaatt aacggattgt gtcaccctct ataccggtca gcaaacttta tttgatgcct
     3661 atggtgtaga aaaggcaatc caaaaggcat tggataaacg ggttgattta aaatcggggg
     3721 gctatttaat tattgagcaa actgaggcga tgacgactat tgatattaat actggtgctt
     3781 ttgtcggaca tcgtaacctt gcacatacga ttttcaacac aaatattgaa gcaatacagg
     3841 caattgcaca ccagctacag ttgcgtaatt taggtggtat tatcattatt gattttattg
     3901 atatgcaaga ggaagaacat cgtgagcgcg ttttacaatc gttacaagag gcattaaaag
     3961 gcgatcgtgt taaaaccaat gtgaatggct ttactcagct tggcttggtg gagatgacca
     4021 gaaaacgtac aagggagagt ctagagcgaa ttctttgttg cgattgccaa gcttgccaag
     4081 ggcgtggcac agtgaaaact gtagagacgg tttgctatga aattatgcgt gaaattgtgc
     4141 gtgtacatca tttgtttaag acagaaaata ttcacgttta tgcagctaaa gatgttgctg
     4201 aatatttaat taacgaggaa agccacgcat tattggctga gcttcaggcg tttatcggta
     4261 aaaaaattga gattaaactt gagccatatt atcatcaaga gcagtttgat gtggtcgtga
     4321 tgtaatctat tacttggttg aggctaaata gttagcctca atatcgttag ttacaaaaaa
     4381 tttagaaaat ctgaccgctt gtttcctatc caaattcctc aaaataacgt ataatcgtcc
     4441 agttttattt atcctttttt gaaatctaag gtaattcaat gtctgaagta gaaaatcaag
     4501 aattagatct aaacggtgaa atgttagccc gccgtgaaaa attaaacaaa atccgtgagc
     4561 agggtaacgc atttccaaat acattccgtc gtgatgcgtt agccaaagat ctgcacgctc
     4621 aatatgatgc agtggaaggt gaaaccttaa aagaacagaa tattcaagtg aaagttgccg
     4681 gtcgtattat gttaaaacga gtaatgggta aggcatcttt ctttaccatt caagatgtga
     4741 gcggtgcgat tcagctttat gtagctcgtg ataacttaag tgacagcgtt tacgaagaaa
     4801 aagtcagcct ttgggattta ggcgacattg tgggcgtcga aggtgcgtta tttaaaacca
     4861 aaaccggtga attaacggtt cgttgctctg aagttcaact tttaaccaag tctttacgcc
     4921 cgttgccgga taaacatcac ggtttaacgg atttagaaac tcgttatcgt cagcgttatt
     4981 tagacttgat ttctaatgaa gaatctcgcc gtacttttat gatccgctcg caagtagttt
     5041 caggcattcg caaattcttc ttagaaagag actttattga agttgaaacg ccaatgttgc
     5101 aagtgatccc gggcggtgcg gcagcgaaac cgtttatcac tcatcataat gcgttagatg
     5161 tggatatgta tctgcgtatt gctccagagc tttacttaaa acgtttagtt gtgggtggtt
     5221 ttgagcgagt atttgagctt aaccgtaact tccgtaacga aggggtttca gtacgccaca
     5281 atcctgaatt tacaatgatt gagtactacc aagcctatgc ggattatcac gatttaatgg
     5341 ataataccga agaactgtta cgccaattag ccttagatat tcttggcaca actgacgtac
     5401 cttatggtga ttatgtattc gatttcggta aaccgtttga gcgtattacg atgcacgatg
     5461 cgattgtgaa atatggtaac ggcattcaac gtgaagattt agacagcttc gagaaaagtg
     5521 tggaaattgc gaaaggctta ggcattgaaa ttcaaaaatc ttggggttta ggctcggtag
     5581 tcaatgcgat ttttgaagaa gtggcagaac atcaattaat tcagccaact ttcttgatgg
     5641 ctcacccggc agaaatttca ccattagctc gccgtaatga tgaaaaccca gaggtaactg
     5701 atcgttttga gttatttatc ggtggtcgtg aaattggtaa tggtttctca gaattaaacg
     5761 atgcagaaga ccaagcagag cgttttgatg ctcaagttgc ggcgaaagat gccggtgatg
     5821 atgaagcaat gtttaaagat gaagacttta tcgtggcatt ggaacacggt ctgccgccaa
     5881 cagcgggtga aggtttagga attgaccgct tagtaatgat tttcgctaac gccccatcaa
     5941 tccgtgatgt aattttattc ccagcgatgc gtcagaagta atcagaaata gaacaaaagc
     6001 acacttcggt gtgctttttt gtattacaag cggtcaaatt tttaataaaa aatgcaattt
     6061 attttgctaa tccggcttta atcatcggtt caaaaaagtc ataatcaacc aaaaaggcat
     6121 cgtgtccgaa atcggagtga aactcgtgga actccaaatt tacgcctgcc tgttctaatt
     6181 tttgtttgct cttatacacg tcaacttgct tgaatagttg gtcgcttgtt accgctacaa
     6241 aagtgtagtt ggctttaata cgttttaggg cggtttcttc attttcatag ccaagagccg
     6301 ggtcgtataa atctaatgct cggagtaaac ggaggtaagt gttggcatcg aaacgaccta
     6361 aaaatttcgt gccttgatag cttaaatagg attccacttg gaaatggtcg ccccaaattt
     6421 gtccttgctg tttggtttct cgtccaaagg ctttagcaag ttgaatatca gtacggtaag
     6481 tcagcatacc gagcatacgg gcgattttta agccgttatc aggtgcaatg ccatcataat
     6541 agttcccacc gttaaagtgt ggatcgttaa taatagcttg gcgcataacg tggttaaaac
     6601 cgatagcctc ggcacttagg gtaagtgacg aacaaagatt tataatgttg cgaactttat
     6661 cagggtaact aataccccac tgtgttgctt gcattccgcc gaaagatccc cctaccactg
     6721 cgtgaagttg agggatttct aaatagtcca ataatgcttt ttgtacatta acaatatctt
     6781 gcactgtaat aattggaaac aaactaccgt aaggtttatt ggttttaggg ttaatcgaag
     6841 ctgggccggt tgtgccttta caaccgccta atacattact gcaaataaag aaatatttat
     6901 cggtatcaaa cgcaagccct ttgccgatga aatcttgcca ccagcctttc tcggttggtg
     6961 agtcatagta aggcttagca tcaccggtaa gggcgtggca aagcaagacc gcattgcttt
     7021 tctctgcatt taatctgccg taagtctggt aagccactgt gataggggac agttcgcctc
     7081 ctaatgtgag tggtaaaggt acgtcctcaa aaagggtaat ctgctgagcc ataaaaatcc
     7141 tgtttgcaaa aaattctgct tttttgaccg cttgttgtaa tgaccgttaa attagcggtg
     7201 ttcatttcaa ttgtcaagaa tttttagacg tctaaacgtc tagattttta aacggctaaa
     7261 atcaaaat
//
LOCUS       DANLYI010000123         7255 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.123, whole genome shotgun sequence.
ACCESSION   DANLYI010000123 DANLYI010000000
VERSION     DANLYI010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 7255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7255
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.123"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            complement(79..291)
                     /locus_tag="PXD81_001755"
     CDS             complement(79..291)
                     /locus_tag="PXD81_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5537762.1"
                     /translation="MKKNVIRYWLSVPFRIIAVLFFLVILTVRYVGYLLIYPLAFFAA
                     VGSFIVFCQWTYQEWLYYFKRGFAPI"
     gene            complement(341..847)
                     /locus_tag="PXD81_001756"
     CDS             complement(341..847)
                     /locus_tag="PXD81_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HDL5537763.1"
                     /translation="MAHSVNDIVKAHGRRYKARLKIQMIKMQGGVLVPLNEREAEALV
                     SLKNGEQYEIEVIRTRNPAFHRKVFAFLNFCFEYWSADKTEWEFFDERKQFDTFRKNL
                     TVLAGFKEITYTLDGRMRVEAQSLSYGNMEQDEFEQVYKALINAAIKHVFNNTKDENR
                     INQLYSFF"
     gene            complement(831..1046)
                     /locus_tag="PXD81_001757"
     CDS             complement(831..1046)
                     /locus_tag="PXD81_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5537764.1"
                     /translation="MITYKCPKCNGELEDLSINDEWGWFLDEPYRCNGHYTGRFPNIS
                     RDSTLNRTKSCGYFSKEEVKKVNGTQR"
     gene            complement(1137..1673)
                     /locus_tag="PXD81_001758"
     CDS             complement(1137..1673)
                     /locus_tag="PXD81_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HDL5537765.1"
                     /translation="MTDFDKNIKYKIIYADPPWKYKDSGCNGSAENHYTTMNIKEICD
                     LPVQDISDKDSILFLWVTYPMLPEGLKLIEAWGFNYKTIGFQWVKTNKKNKNSFFFGL
                     GRWTRGNTECCLIATKGKPSRVSNKVSQLIIEPIQHHSKKPDIVREKIVELMGDLPRI
                     ELFARNKTQGWDVWGDEV"
     gene            complement(1670..3031)
                     /locus_tag="PXD81_001759"
     CDS             complement(1670..3031)
                     /locus_tag="PXD81_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase"
                     /protein_id="HDL5537766.1"
                     /translation="MSQNLKNITYEVEYLLVGALLKSGLNSKARDVLSWLEPEMFATF
                     QLGVIYEAIRKQALKDNVIDMLLLNTDYGQDFATLAEIMKNTISGANLDGYAEKVRQY
                     HQRREAQRVFLDVAGELQNARDEQLDAITSTGLAHLSKLLQRGGKVKPIDMNDLLEGY
                     LELFQERAKPSFKERLLFTGIEALDGKLGGINDTDICIVAGRAGNGKTETAITFTKNI
                     IENKGSVLFFSLEMSKEQIMDRLIASASGVNSVKLRNPEMMNDEDFARMGTAIQPLQN
                     QQLYIVDKSGLTAEEIVAIAESHIQEYGKVSAVVIDYIGLVRHGKLDGKINRTYQIGE
                     SMERFKTFCKNNHTPMILLAQLNRNADGSRPTNADLRDSGSLEQDASQIIMVHNQRNK
                     DGEPAPYTEWIVTKNRFGANGTVYMQFQQGRFVECDQAEAREYFQPKETKTSSNKRYG
                     AMQ"
     gene            complement(3028..3897)
                     /locus_tag="PXD81_001760"
     CDS             complement(3028..3897)
                     /locus_tag="PXD81_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HDL5537767.1"
                     /translation="MNAQPKQINPILKLHPKQSEAKKVSVDDGYTQIPNELLKAILRS
                     GVLGWKGSYLLATVLKTLSWHKESDWFTHSQVCEMMNIEPTKYHINQLSAARKELIRE
                     NILFEDGKETGVNLSVFEWKMVYPEKVGSSRNNRELIPEKVGNGYPEKVGNTKETITK
                     EKINNEFTNVNSVQQPETSLPKKTKSEPVDYQGVMEEFNRAVEDTPIPQIRAMSDERK
                     RSVHALAKILRKEFGGYTRQHFADYFNDFVRQASSRKDRFYFGGLDGSAWVANFGYII
                     RSKTFYKTWEDSL"
     gene            complement(3872..4042)
                     /locus_tag="PXD81_001761"
     CDS             complement(3872..4042)
                     /locus_tag="PXD81_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5537768.1"
                     /translation="MKNQRNDTQKSSLWQEFEYRKRLKNLIESGLSVAEIETRSQQIY
                     EELKSECTTEAN"
     gene            complement(4206..4466)
                     /locus_tag="PXD81_001762"
     CDS             complement(4206..4466)
                     /locus_tag="PXD81_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5537769.1"
                     /translation="MPRNELTKNARAIADLIHRKSATVTHKKLARAIGLSESQFSRTF
                     ADNVEMVAVIIDYLSIELADKDELLELKEKRYKRINLEEATT"
     gene            complement(4487..4693)
                     /locus_tag="PXD81_001763"
     CDS             complement(4487..4693)
                     /locus_tag="PXD81_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005656653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL5537770.1"
                     /translation="MNKIAEIRNQIGISQAQLAVQIGWGQPRIANYETGNRTPSIYVA
                     QKIVEGLNELGANVRIDDVFPVGN"
     gene            4823..5482
                     /locus_tag="PXD81_001764"
     CDS             4823..5482
                     /locus_tag="PXD81_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HDL5537771.1"
                     /translation="MTTLGSRIKAYREQLGISQYEVAERCTSIDNRDKNNKWGQSRIA
                     NYERNNRTPDLDDIEILSKVLGVSPETLAFDTNVQLARPTKSNSYPLISNIQAGLWTE
                     AFDFKDSEGYDYIDTEIDAGPDAFFLRISGMSMEPKFSEGDLVLIDIRKRPHPGDYVA
                     AVNGTGEATLKRYRELGEWSESGNPHFELIPLNPDFPTLSSMKQDIRIIGVAVEHRSY
                     L"
     gene            5533..5913
                     /locus_tag="PXD81_001765"
     CDS             5533..5913
                     /locus_tag="PXD81_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5537772.1"
                     /translation="MNNKRIKPEQFADFITQRTGRNLTCPICNSQEHYLHDGYDTFTE
                     LCGEKIIGVPTIPYRIEPSPDEVMKFAAPEFHSLHFNEREGTKYWLDQKARTAVIVTC
                     VCCNNMLFFDRERILEWIKGSQNE"
     gene            5918..6403
                     /locus_tag="PXD81_001766"
     CDS             5918..6403
                     /locus_tag="PXD81_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5537773.1"
                     /translation="MIQNDPRDAIILSLLANAVYGGHNNSQISYQETEEVNLYARLRQ
                     VEGDVREIKSNYLTKEAFYKAGGSTLIALVVTAGIALWTVYSHLDTKVEARFSKIDDK
                     FVQVETNIKNLDIRLTKVESRLDNVEQRLDNVEQRLGNMEKKIDGVDNKLDLLLQQFK
                     R"
     gene            6535..6717
                     /locus_tag="PXD81_001767"
     CDS             6535..6717
                     /locus_tag="PXD81_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HDL5537774.1"
                     /translation="MKYSEFLRYLLAQGCEIENHRRGSHRKVTLNGKQSVFPYHGSKE
                     IGTGLVNKIKKDLDLK"
     gene            6756..7175
                     /locus_tag="PXD81_001768"
     CDS             6756..7175
                     /locus_tag="PXD81_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012621799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HDL5537775.1"
                     /translation="MLRYPVEITPDDNGTYLVTCPDIPEMASVGEDLEEALLEAQDGL
                     ATALEFYFDDRREIPMPSPIKEGQHTVNLTVLQSMKVFLLNEMIKQGVRKAEMARRLD
                     VHLPQIDRLLDFNHSTKVEFVEKAYGKLNQHFTILPH"
BASE COUNT         2130 a         1541 c         1289 g         2295 t
ORIGIN      
        1 cacgaatgcg atagtttcaa aaaattttgt catcttactt ctccttcccc cgaattcgag
       61 ggaattaata aacacacact aaatcggagc aaaaccgcgc ttaaaataat aaagccattc
      121 ttgatatgtc cattgacaga aaacaataaa actaccgact gctgcaaaga atgctaatgg
      181 ataaatcaat aaataaccta catatctaac agtaagaatg actaagaaaa aaagcactgc
      241 aataattctg aatggcacgc taagccaata tctaataaca ttctttttca ttttaatctc
      301 ctgcaattaa acctaattaa atatggcgat ttcgccttaa ttaaaaaaac gaatacaact
      361 gatttatcct attctcatct ttggtattgt tgaaaacgtg cttgatagct gcattaatca
      421 atgctttgta aacctgctcg aactcgtctt gttccatatt tccgtaacta agactttgag
      481 cttctacacg catacgccca tctagggtgt atgtgatttc tttaaatcct gccagaacag
      541 tcagattttt tcggaaagta tcaaactgtt tcctctcatc aaaaaactcc cattcggttt
      601 tgtctgccga ccaatattca aagcaaaaat ttaaaaaggc gaagaccttc cgatgaaagg
      661 ctggattgcg agtgcgaata acctcaattt catattgctc gccattttta agtgatacca
      721 atgcttcagc ttcacgctca ttaaggggga ctaaaacgcc tccttgcatt tttatcatct
      781 gtattttcaa tcttgcttta taacgcctgc cgtgggcttt tacgatgtca ttaacgctgt
      841 gtgccattta ctttcttaac ctcttccttg ctaaagtacc cacaggattt tgtgcgattt
      901 aacgtgctat ctcggctaat gtttggaaac cgccctgtat agtgtccgtt gcaacgatac
      961 ggctcatcta aaaaccaacc ccattcatca ttaatactta aatcttcaag ctctccgttg
     1021 catttggggc atttataagt aatcatctaa accgtcccaa ccttcgttat ccttcacaac
     1081 cgccaacctt tttaataaaa ccctaccgca ctttgggtaa caatagcttg aaacaatcac
     1141 acctcatccc cccaaacatc ccaaccttgg gttttatttc tggcgaacaa ctctattctt
     1201 ggcaagtcac ccatcaactc aactatcttt tctctgacaa tgtcaggctt tttactatga
     1261 tgttgtattg gctcaataat taattggctc actttattgc ttaccctcga tggctttcct
     1321 tttgttgcaa ttaaacagca ttcggtatta cctctagtcc atcgtcccaa gccaaagaaa
     1381 aaagaattct tattcttctt atttgtcttc acccattgaa accctattgt tttatagttg
     1441 aacccccacg cctcaatcaa ctttaagccc tcaggtaaca tagggtaagt aacccacaaa
     1501 aacagaatag aatctttgtc tgaaatatct tggacgggta aatcacatat ttctttgata
     1561 ttcattgttg tgtaatggtt ttctgcactc ccattacagc cactatcctt atacttccaa
     1621 ggcggatctg cgtaaataat tttgtatttt atatttttat caaaatctgt cattgcattg
     1681 ctccatagcg tttgtttgat gatgttttag tttctttcgg ctggaaatac tcccgcgctt
     1741 ctgcctggtc gcattctacg aacctaccct gctggaattg catataaacc gtgccatttg
     1801 ctccaaagcg atttttagta acaatccatt ctgtataggg ggctggctcg ccgtctttgt
     1861 tgcgttggtt atgcaccata atgatttggc ttgcatcttg ttcaagactg ccactgtctc
     1921 gcaaatctgc gttggttggg cgactgccat ctgcattgcg gttaagctgt gccaataaaa
     1981 tcatcggtgt gtgattgttt ttgcagaaag ttttaaaccg ctccatactt tcaccgattt
     2041 gataggttcg gttaattttg ccgtctagtt tgccgtgtcg aaccaaaccg atataatcaa
     2101 tcactaccgc actcactttg ccgtactctt gaatatggct ctctgcaatc gccacaatct
     2161 cttctgcggt taatccgctt ttatcaacaa tgtacaactg ctgattttgt aaaggctgaa
     2221 tcgccgttcc cattcgggca aaatcttcat cattcatcat ttccggattg cgaagtttta
     2281 ctgaatttac accgcttgca ctggcaatca gcctatccat aatctgctct ttgctcatct
     2341 ccagcgagaa gaacagcacc gaacctttat tctcgataat gtttttagta aacgtgatgg
     2401 ctgtttcggt tttaccgtta ccggcacgtc ctgctacaat gcaaatatcg gtatcgttaa
     2461 taccgcctag tttcccatct aatgcttcta taccggtaaa caacaaacgc tctttaaaac
     2521 ttggttttgc acgttcttgg aacaattcca aataaccttc gagcaaatca ttcatatcaa
     2581 tcggtttcac ttttccgcca cgttgtaata atttgcttag gtgagctaac ccggttgaag
     2641 tgatagcatc taactgctcg tctcgggcat tttgcaactc acctgcaacg tctaaaaaca
     2701 ctcgctgggc ttctcggcgt tgatgatatt gccggacttt ttctgcatag ccgtctaagt
     2761 ttgcaccgct gatcgtgttt ttcattatct ctgccaacgt ggcaaaatct tgtccgtagt
     2821 cggtattgag taacagcata tcgatcacgt tatctttcaa cgcctgcttg cggatagcct
     2881 cataaatcac cccaagttga aaggtagcga acatttcagg ctctaaccag ctcaatacgt
     2941 ccctagcttt cgagttaaga ccggatttca gcaatgctcc aaccagcaaa tattcaacct
     3001 cgtaagtgat atttttcaaa ttttggctca tagcgaatct tcccaagtct tataaaacgt
     3061 tttcgaacga atgatgtagc cgaaatttgc tacccaagcc gagccatcaa gcccgccaaa
     3121 ataaaatctg tctttccggc tacttgcctg tcggacaaaa tcattgaagt aatcggcaaa
     3181 gtgttgtctg gtgtagccac caaattcttt tcgcaagatt tttgctaatg cgtgaaccga
     3241 tcgctttcgc tcatcactca tcgcccgaat ttgcggaatc ggtgtgtctt ccacagcacg
     3301 attaaattct tccatcactc cttgataatc gaccggctca gattttgttt ttttcggcaa
     3361 agaggtctcg ggctgttgca cagaatttac gttagtaaat tcattattta ttttttcttt
     3421 tgtaatagtt tcttttgtgt tccctacttt ttcgggatac ccattcccta ctttttcggg
     3481 aattaattcc ctattatttc gggaactccc tactttttcg ggatatacca ttttccattc
     3541 aaaaacactg agatttaccc ctgtttcctt accatcttca aacaagatat tttcacggat
     3601 aagttctttt cgagcagcgg aaagttgatt aatatggtat tttgtcggct caatattcat
     3661 catttcacac acttgggaat gcgtgaacca atcactttct ttatgccaag acaatgtttt
     3721 taagacggta gccagtaagt aagagccttt ccaacctaaa acacccgatc gcaaaattgc
     3781 ttttaataac tcattcggaa tttgtgtata accatcatca acactcactt tcttagcctc
     3841 tgattgttta gggtgcaatt ttaaaattgg gttaatttgc ttcggttgtg cattcacttt
     3901 tcagctcctc ataaatttgt tgtgaacggg tttcaatttc ggctacggat aaaccgcttt
     3961 caatgagatt ttttaaccgt tttcgatatt caaactcttg ccataaggag cttttttgtg
     4021 tatcattgcg ttgatttttc aaaaggtaac tccttgtaaa ttaccaccgt tccagcggtg
     4081 gttttttaat tgttaaaaac ccaccacgca agcagcataa aaattgccac tctaataaaa
     4141 attgaatttt tactgtggta gaatgatttg aatttttcta aaaaattttt cattgttggt
     4201 aacttctatg ttgtcgcttc ttcgagattt attcgcttat atcgtttctc tttcagctct
     4261 agtaactcgt ctttatccgc aagctcaata cttaagtaat caataatcac cgccaccatt
     4321 tccacattat ctgcaaatgt acggctaaat tgtgattcac tcagtccgat tgctcttgct
     4381 agtttcttat gcgtcaccgt tgcggatttt cgatgaatca aatccgcaat cgctcttgca
     4441 ttttttgtta attcattgcg tggcattgca agcctcttgg ggtaaattag ttgccaacgg
     4501 ggaaaacgtc atcaattcgt acgtttgcac ctaattcgtt caatccctca acaatttttt
     4561 gagcaacgta aatagatggg gtacgatttc cagtttcata gttagctatt cttggttgcc
     4621 cccaaccaat ctgaacagct aactgagctt gacttatacc aatttggttt cgtatttcag
     4681 ctatcttatt catacaaatt tctctttgtg atttatatct tttaattaaa tcacaaaatg
     4741 aactaattgt aaatttcatt ttgtgattca caaggatata acggagcgtg ttaaaatgag
     4801 gaaaatctaa taggagagtg ctatgacaac cttaggtagt agaattaagg cttacagaga
     4861 acagctagga atcagccaat atgaagttgc tgaaagatgt acatcaatag ataatcgaga
     4921 taaaaataac aaatgggggc aatctcgcat tgctaattat gagagaaaca atcgtactcc
     4981 cgatcttgat gatattgaaa tactcagcaa agtgcttggt gtttctccag aaacacttgc
     5041 ttttgataca aacgttcagc ttgccagacc gacaaaatcg aactcatatc cgttgatcag
     5101 caatatacaa gcgggtttat ggacagaagc ctttgatttc aaagattcgg agggctacga
     5161 ctatatcgat acggaaattg atgccggacc ggacgctttc tttttaagaa tttccggaat
     5221 gtcgatggag cctaaattta gtgaaggcga tctggtgtta atcgatattc gcaaacgtcc
     5281 tcacccgggc gattatgtag cagcggttaa tggcacaggt gaggctactt taaaacgtta
     5341 tcgggagtta ggcgaatggt ctgaatccgg caatcctcat tttgagctaa taccgcttaa
     5401 ccccgacttt ccaacactca gctcaatgaa acaagatatt cgtattattg gtgtcgctgt
     5461 tgagcataga agctatttgt aaagtccacc aaagctaaca gttgaatttt attgggtaga
     5521 aaggaagtta aaatgaataa taaacgcatt aaacctgaac agtttgcgga ttttatcacg
     5581 caacgtactg ggcggaattt aacttgccct atttgtaata gccaagaaca ttatttacac
     5641 gatggctatg acacttttac ggaattatgc ggagagaaaa ttatcggtgt tccaaccatt
     5701 ccataccgaa tagagccatc tcctgatgaa gttatgaaat ttgcagctcc tgaatttcat
     5761 tctttgcatt tcaatgaacg ggaaggaaca aaatattggt tagaccaaaa agcgagaaca
     5821 gccgttattg tcacttgtgt ttgctgtaat aatatgcttt ttttcgacag agagagaatt
     5881 ttagaatgga ttaagggctc acaaaatgaa tagaccaatg attcaaaatg atcctagaga
     5941 tgcaatcata ctatccttac ttgcaaatgc agtatacggc gggcataata attcacaaat
     6001 tagttatcag gagacggaag aagtgaattt atatgctcga ttaagacaag ttgaaggcga
     6061 tgtaagagaa atcaagtcaa attatttgac gaaagaggct ttttataaag ctggtggctc
     6121 tacactcatt gcattagtag ttactgctgg aattgcatta tggacagtat attcacacct
     6181 tgacaccaaa gttgaagctc gattctcgaa aattgatgat aagtttgtcc aagtagagac
     6241 caatatcaaa aatcttgata ttcgcctcac taaagttgaa tctagattag ataatgtcga
     6301 acaacgacta gacaatgttg agcagcgact aggcaatatg gagaagaaaa tagatggggt
     6361 tgataataaa ctcgatctgt tactacaaca attcaaaaga taaaataata ttccttgaat
     6421 aaaccgccct cgtggcggtt ttcttttctg ctacaaaaaa aacacaacaa aaatgttgta
     6481 ataccaacaa aaatgttgta ttatttcctt gctgttttga taaggaggta tcttatgaaa
     6541 tacagcgagt tcctgaggta cttacttgct caaggttgcg aaattgaaaa ccataggcga
     6601 ggcagtcatc gaaaggtaac actaaacgga aaacaatcgg tttttcctta tcacgggagc
     6661 aaagagattg gtacaggttt agtaaataag attaagaaag acttagatct taaataacaa
     6721 agtccctcga aagaggggct tttctaggag tttatatgtt acgctaccca gttgaaatta
     6781 cccctgatga taacggcaca tatcttgtga cttgccctga tattcctgaa atggcaagcg
     6841 taggtgagga cttagaagaa gccctattag aagcacaaga tggcttagcc acagcccttg
     6901 aattctattt tgatgataga cgagaaatac caatgccaag cccaattaaa gagggacaac
     6961 atacggttaa tcttaccgtg ctgcaatcaa tgaaagtatt tctcttaaat gaaatgatta
     7021 aacaaggtgt acgcaaagca gaaatggcaa gacgattaga tgtacactta ccacagatag
     7081 atcgcctatt agattttaat cattccacaa aagtagaatt tgttgagaaa gcctatggta
     7141 agctaaatca acactttact attctccctc actaataaaa taaccgccta cgggcggttt
     7201 tctttttcct aaaatctgca aaattctctt caaaaacgac cgcttgcaac tcaat
//
LOCUS       DANLYI010000124         7159 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.125, whole genome shotgun sequence.
ACCESSION   DANLYI010000124 DANLYI010000000
VERSION     DANLYI010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 7159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7159
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.125"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            92..1282
                     /locus_tag="PXD81_001769"
     CDS             92..1282
                     /locus_tag="PXD81_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000616649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine--oxo-acid transaminase"
                     /protein_id="HDL5537776.1"
                     /translation="MSTSQQLIQQTEHFGANNYLPLPIVISKAKGIWVEDPEGNRYLD
                     MLSAYSAVNQGHCHPKIVQALKDQADKVTLTSRAFYNDQLGIWYEKICKLANKEMALP
                     MNTGAEAVETAIKTARRWGYDVKGIAENQAEIIACVGNFHGRTMAAVSLSSDPDYKRG
                     FGPMLGGIKLVPYGDLEALKAAITPNTAAFLVEPIQGESGILIPPEGFLEQAYELCKE
                     NNVLFIADEIQAGLGRTGKLFATEWEGFEPDIYILGKALGGGVFPISCVLANRDILGV
                     FNPGSHGSTFGGNPLACAVSIASIDVLLEEKLAERSRELGEYFLAELKKIQNPIIKEV
                     RGRGLFIGLELHQPARPFCEKLKEKGLLCKETHDTVIRFAPPLVIEKFNLDWALEKIR
                     EVLA"
     gene            1357..2868
                     /gene="nhaC"
                     /locus_tag="PXD81_001770"
     CDS             1357..2868
                     /gene="nhaC"
                     /locus_tag="PXD81_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HDL5537777.1"
                     /translation="MLILQTFSKNQPLVKGGFMSNRTEITDQNIRIPFRYAILPVIIM
                     VSIMAVAIIKFEASPHVPLLIGAISAAVIAMAHGYRWETIEKGIYRGIRMALPAIVII
                     IMIGLTIGAWLGGGIVATMIYYGLKLISPSLFLVTISIICAIVTLAIGSSWSTMGTIG
                     VAAMGIGFSLGIPAPMVAGAVISGAYFGDKMSPLSDTTNLAAGITETNLFEHIRHMFI
                     TTVPAFLIALSVYFYFGMEFRAENLDLAKIEDIMQAMQEHFLISPWLLLVPLLVVVLV
                     AKKVPALPALAAGIILGWLCHVFVQGGDVSVAVITLHDGYKIESGHEMIDKLFNRGGI
                     ESMMYTISLTIVAMTFGGIAEQAGMLHSVVEKILTLAKSAGSLISATIVSAFITNVTT
                     SEQYISILLPGRMYIKAYRDKGLSSRNLSRALEDGGTVTSVLVPWNTCGVYAFSMLQV
                     SAFEYAPYAIMNWLIPLIAIGLATLNIKIERLPPEELAKEPEEITLPKRGCCP"
     gene            2985..6584
                     /gene="putA"
                     /locus_tag="PXD81_001771"
     CDS             2985..6584
                     /gene="putA"
                     /locus_tag="PXD81_001771"
                     /EC_number="1.2.1.88"
                     /EC_number="1.5.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011848319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional proline dehydrogenase/L-glutamate
                     gamma-semialdehyde dehydrogenase PutA"
                     /protein_id="HDL5537778.1"
                     /translation="MMSYQLLPNTTIRPELSRHYRFDEQTLVQNLIEQAHCDEQADNI
                     KKLTTQLVGKVRTARQKASGVDALMHEFSLSSQEGVALMCLAEALLRIPDTATADKLI
                     RDKIAKGDWRSHTGNSPSLFVNAAAWGLVITGKLVSTHSESSLSSALSRLIAKGGEPL
                     IRKGVDVAMRMLGKKFVTGETIQQAIKNGEKRFAMGYRYSYDMLGEAALTAEDAKRYY
                     NDYVASIHAVGATSKGLGVYKSSGVSVKLSAIHPRYSLSQHQRVMDELYPRLKELFLL
                     AKKYDIGLNIDAEEADRLELSLDLMDRLLADPDLAGFNGIGFVVQAYQKRCPYVIEHL
                     IETARKHNRQLMIRLVKGAYWDTEIKRAQMDGAEGYPVFSRKVHTDLNYIVCAKKLLA
                     AQDVIYPQFATHNAQTLATIYHLAQGKRFEFQCLHGMGESLYDQVVGSDNLNVQCRIY
                     APVGTYQTLLAYLVRRLLENGANSSFVSQIVDENLSVDDLAQAPVQKAELTNGTMHPK
                     IPLPVDLFDDNRVNSKGYDLTDAVSLQNLENALNELTKQHYRAEPQLANDNTSGQESR
                     KIYNPANQAELVGEVIDATENDMEQAFESAAAFQSEWANTSPAVRAECLEKMADLMEA
                     NMPQLFDLAVREAGKTLNNAIAEVREAVDFCRYYAKEVRNNIEAYKNPRGIVVAISPW
                     NFPLAIFVGEVSSALVAGNVVLAKPAEQTSLMAHYAVSLFYQAGVPKAALQCLPGRGK
                     TVGVALVADHRVNGVIFTGSTETAKNINRNLHKNEKIIPLIAETGGQNAMIVDSSALG
                     EQVVIDVLNSAFDSAGQRCSALRVLYLQRDVADHILTMLKGAMAELKVGLPQHLTTDV
                     GPVIDKVAQQRLLDHIENLKKSAKSYYQVAISSEIAENGIFVPPTLLEIDHISQLKHE
                     VFGPVLHVIRFDADKLDQVIADINSTGFGLTSGLHSRIDETVDLWLKSVHAGNLYVNR
                     NTVGAVVGVQPFGGMGLSGTGPKAGGPLYLQRLVNRQEWTLNGLQGNAQAVDFDLNFA
                     IRKVLSGAEQQTALDLAKSLARRSPLGKTYPMQGITGEKNEMIFVARPQIGISKGNLA
                     SQVQALIAIVTVGSQAVAEKSSELAKHASAFGKKLQVVDDFNSLDHLSTLIVLDLLSS
                     EEKLHHAERKGAILTYIEGLDLALSLLPLVHEKAISINTAAAGGNASLMSEMD"
     gene            6693..>7159
                     /locus_tag="PXD81_001772"
     CDS             6693..>7159
                     /locus_tag="PXD81_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595-like element ISMha3 family transposase"
                     /protein_id="HDL5537779.1"
                     /translation="MRKSRLSQYKQHKLIELFVAGVIARTAAELVNVNKTTAAYYFHR
                     LRLLIYQHSPHLEMFEGEIEIDESYFGGHRKGKRGRGAAGKTAVFGLLKRDGKVYTVV
                     VPNTQSATLLPIIREKVKPDSLVYTDFYRSYDVLDVSEFNHFRINHSTHFAEN"
BASE COUNT         2023 a         1508 c         1704 g         1924 t
ORIGIN      
        1 agatcataaa atcaacaaaa agtccgtgct ttttagctaa agattgttaa actcttttgt
       61 gttacaagtt tttaacataa aggagttttt tatgtctact tctcaacaac tcatccaaca
      121 aaccgaacat ttcggtgcca ataactacct acctctacct attgttatta gtaaagctaa
      181 aggcatttgg gttgaagatc ctgagggtaa tcgctatttg gatatgctta gtgcctactc
      241 agcggttaat caagggcatt gtcatccaaa aattgtacag gcactaaaag atcaagcaga
      301 caaagtaacg ctgacctctc gagcatttta taacgatcaa cttggtattt ggtatgaaaa
      361 aatctgcaaa ctcgctaata aagagatggc attaccgatg aatacgggtg cagaagcggt
      421 agaaacagcg attaaaactg ctcgccgttg gggttatgat gtgaaaggaa ttgcagaaaa
      481 tcaagcagaa atcatcgctt gtgttggcaa ttttcacgga cgaacaatgg ctgcagtatc
      541 tctctcgtct gatccggatt ataaacgtgg tttcgggccg atgttaggtg gcatcaaact
      601 tgttccatac ggtgatttag aagcattaaa agctgcgatt acaccaaaca cagcagcgtt
      661 tttggttgag ccaatccaag gtgaatctgg cattcttatt ccacctgaag gtttcttgga
      721 acaggcttat gaactatgca aagagaataa tgtgctgttt attgctgatg aaattcaggc
      781 aggtcttggg cgtactggca agttatttgc aacagaatgg gaaggctttg agccagatat
      841 ttatattcta ggtaaagcac ttggtggcgg ggtgttccca atctcttgtg tgttagcaaa
      901 tcgtgatatt ttaggcgtat tcaatcctgg ctcgcacggt tcgacatttg gtggtaatcc
      961 attagcctgt gcggtatcta ttgcctcaat tgatgtactg ttggaagaaa aacttgcaga
     1021 gcgttcccgt gagttaggtg aatacttctt ggcagagctg aaaaaaatcc aaaatccaat
     1081 tattaaagaa gttcgtggac gtggactgtt tattggcttg gagttacatc aaccagcacg
     1141 cccattctgc gaaaaactca aagaaaaagg cttactctgc aaagagaccc acgatacagt
     1201 tatccgcttt gctccccctt tggtgattga aaaattcaat ttggattggg cattggagaa
     1261 aattcgggag gtgttggctt aattgtcatt ttctctatat tttatcctgc caaatcgcct
     1321 ttaacccttt ttggcaggaa gcaaaacagt tgtaaaatgc tgattttgca aactttttcc
     1381 aaaaatcaac cgcttgtaaa aggtggcttt atgtcaaaca gaacagaaat aacagaccaa
     1441 aatatccgaa tcccgtttcg ctatgctata ttgcctgtga ttattatggt atcgattatg
     1501 gcggtggcta tcatcaaatt tgaagcctca cctcacgttc cgttgcttat tggggcaatt
     1561 agtgccgctg tgattgcaat ggcacatggc taccgttggg agacaattga aaaaggaatt
     1621 tatagaggca ttcgtatggc tttaccagcg attgttatca ttattatgat tgggctaacc
     1681 atcggagctt ggctaggtgg tggaattgta gctacgatga tttactacgg cttgaaattg
     1741 atctctccgt cgctgttttt agtaactatc tcaataattt gtgctattgt gacccttgcg
     1801 attggtagct cgtggtctac aatgggaaca atcggtgtag cggcgatggg gatcgggttt
     1861 agtctcggca ttccggcacc gatggttgcc ggtgcggtga tctcaggagc atatttcggc
     1921 gataaaatgt cgccactgtc agacactacc aatctggcag cagggattac cgaaaccaat
     1981 ctgtttgaac atatccgcca tatgtttatc actaccgtgc cggcgttcct gattgcgtta
     2041 tctgtctatt tctattttgg tatggaattt agagccgaaa atctcgatct tgctaaaatt
     2101 gaagacatta tgcaggcaat gcaggaacat ttcttgattt cgccttggtt gctattagtg
     2161 cctttattag tcgtggtgtt ggtggcaaaa aaagtgcctg ccttaccggc attagctgca
     2221 gggataattc ttggttggct ctgccacgtt tttgtgcaag gcggtgatgt cagcgtagca
     2281 gtgattactc tgcacgacgg ctataaaatt gagagcggcc acgagatgat tgataagctg
     2341 ttcaaccgtg gtgggatcga gtcgatgatg tacaccattt cccttacgat tgttgcgatg
     2401 acttttggtg gcattgcaga gcaagctgga atgttacatt cagtagtgga aaaaatcctt
     2461 acccttgcaa aatcagcagg tagcttaatt agtgctacga ttgtatcggc atttattacc
     2521 aacgtaacga catctgagca atatatttct attttattac caggcagaat gtatataaaa
     2581 gcctataggg ataagggact aagttcgaga aacctttctc gtgcattgga agacggagga
     2641 acagttactt cggttcttgt accttggaat acctgtggtg tttatgcgtt ctcaatgttg
     2701 caagtgagtg cttttgaata tgcaccgtat gcaataatga attggctcat tccactgatt
     2761 gcgattggct tggcaacctt gaatatcaaa attgagcgat tgccaccgga agaattagcc
     2821 aaagaacctg aggaaattac tctgccaaaa cgtggttgtt gtccataatt tatctaaata
     2881 gtatttaata acaataggtt aagttaaata acaagcggtc ggttttatct taaaatttgc
     2941 aagtttatac ataaatttga tcgcttgctg tatagaaagg aaaaatgatg tcataccaac
     3001 ttttacctaa caccactatc cgtcctgaat tgagccgtca ttaccgcttt gatgagcaaa
     3061 ccctggtgca aaatctgata gaacaagcac attgtgatga acaggctgat aacattaaaa
     3121 agctcactac ccaattagtc ggtaaagtcc gaactgcacg ccagaaagca agcggggttg
     3181 atgcgttaat gcacgaattt tcactctcca gccaagaggg ggtggcgttg atgtgccttg
     3241 ccgaagcatt actacgcatt cctgacaccg ccactgcgga taaattaatt cgagacaaaa
     3301 ttgccaaagg ggattggcgt tcacacaccg gcaacagccc ctctctgttt gtgaatgcag
     3361 cggcgtgggg cttggtgatt accggcaaat tggtttcaac ccacagcgaa agctcactct
     3421 catcagcact ttcacgcctg atcgccaaag gcggagaacc gctgatccgc aaaggtgtgg
     3481 acgtagcaat gcgtatgctc ggcaagaaat ttgtaacggg cgaaaccatt caacaggcga
     3541 ttaaaaacgg cgaaaaacgc ttcgcaatgg gttatcgtta cagctacgat atgctgggcg
     3601 aagcggcact caccgccgaa gatgccaaac gctattacaa cgactatgtt gcctctattc
     3661 acgctgtggg tgccacctcg aaaggcttgg gcgtgtataa atcctcgggt gtgtcggtca
     3721 aactctccgc cattcaccct cgttattccc tttcccaaca ccaacgtgtg atggacgaac
     3781 tctatccacg tctgaaagaa ctgttcttgc tggcgaaaaa gtatgacatc ggcttgaaca
     3841 ttgatgccga agaagccgac cgcctcgaat tgtcgcttga tttgatggac agactgcttg
     3901 ccgaccctga tttggcaggg tttaacggca ttggttttgt ggtgcaggcg taccaaaaac
     3961 gttgcccgta tgtgatcgag catttaattg agaccgcccg taaacataat cgccagctaa
     4021 tgattcgctt ggtaaaaggc gcatattggg acactgaaat caagcgggcg caaatggacg
     4081 gtgcagaggg ctatccggtc ttctcccgta aggtgcatac cgatttgaac tacatcgttt
     4141 gtgccaaaaa attgctggcg gcgcaagatg tgatctatcc gcaatttgcc acccacaacg
     4201 ctcaaactct cgccaccatt taccacttgg cacagggcaa acgttttgag tttcaatgct
     4261 tgcacggtat gggagagagc ttgtacgacc aagtggttgg cagcgataat cttaacgtgc
     4321 aatgtcgcat ttatgcccct gtcggtactt atcaaaccct gcttgcctat ttagtacgtc
     4381 gcttgttgga aaacggagcg aacagctcat ttgttagcca aattgtggat gaaaatctca
     4441 gtgtagatga tctggctcaa gcccctgttc aaaaagcaga actgaccaat ggcactatgc
     4501 acccgaaaat tccattgcca gtcgatttat ttgacgacaa tcgtgttaat tccaaaggct
     4561 atgatttaac tgatgcagta agcctacaaa atttagaaaa tgccctcaat gaacttacca
     4621 aacagcatta ccgtgctgaa ccacagctgg caaatgataa tacaagcggt caagaaagcc
     4681 gaaaaattta caatccagcg aaccaagctg aattagtcgg cgaagtgatt gatgccaccg
     4741 aaaacgatat ggaacaggcg tttgaaagtg cggcggcgtt ccaaagtgaa tgggcgaata
     4801 cttcccctgc ggtgcgtgcc gagtgcttag aaaaaatggc ggacttgatg gaagccaata
     4861 tgccacaact gttcgatttg gcggtgcgtg aggcaggcaa aacgctcaac aatgcaattg
     4921 ctgaagtgcg tgaagccgtc gatttctgtc gttattatgc caaagaggta cgcaacaaca
     4981 ttgaagcata caaaaatccg cgtggcattg tagtagcaat tagcccgtgg aactttccgt
     5041 tagcgatttt tgtgggtgaa gtttcatctg cgttggtggc aggcaatgtg gtactagcaa
     5101 aaccagctga acaaacttcg ttaatggcac attatgctgt cagccttttc taccaagcag
     5161 gtgtgccgaa agcggcgttg caatgcttac cgggtagagg aaaaacggtt ggggtggcgt
     5221 tggtggcaga tcatcgtgtg aacggcgtga tttttactgg ttcaacagag acagctaaaa
     5281 acattaaccg caatttgcac aaaaacgaga aaattatacc gcttattgcc gaaactggcg
     5341 gacaaaatgc aatgattgtt gatagctcgg cactgggcga gcaggttgtg atagatgttt
     5401 taaactccgc atttgactca gcagggcaac gttgttcggc attgcgtgtg ctgtatctgc
     5461 aacgtgatgt tgccgatcac attctcacaa tgctcaaagg agcaatggcg gagttgaaag
     5521 ttggtcttcc acaacactta actaccgatg tcggaccagt aatcgataaa gtcgcacaac
     5581 aacgcttgct cgatcatatt gaaaatctca aaaaatcggc gaaaagttac tatcaggtgg
     5641 ctatctcctc tgaaattgcc gaaaatggta ttttcgtacc gccaacgctg ttagaaatag
     5701 atcatatcag ccagcttaaa cacgaagtat tcggaccggt gctacacgtt atccgttttg
     5761 atgcggataa actcgaccaa gtgattgccg acataaactc cacaggtttc ggtctaacca
     5821 gcggtttgca tagccgaatt gatgaaacgg tggatttatg gttgaaatct gtccacgctg
     5881 gaaatctgta tgtaaaccgt aacacggtag gggctgtggt cggcgtacag ccattcggtg
     5941 gaatggggtt atcaggaact gggccgaaag ctggcgggcc gctctatcta caacgtttag
     6001 taaaccgcca agaatggaca ttgaacggtt tacagggcaa tgcacaagcg gtcgattttg
     6061 acttaaattt tgcaatccgc aaggttctaa gtggagctga gcagcaaacg gcgttagatc
     6121 tggccaaaag ccttgcacgc cgctcgccac tcggaaaaac ctacccaatg cagggtatta
     6181 ccggcgagaa aaacgagatg attttcgtgg ctcgcccaca gattgggatt agcaaaggta
     6241 atttggcttc acaggtgcaa gccttgattg ccattgtaac ggtcggcagc caagcggtgg
     6301 cagaaaaatc aagcgagctg gcaaaacacg cctctgcctt tggtaagaaa ttacaggtgg
     6361 tggatgattt caacagtctc gatcacctca gcacactcat tgtgcttgac ctgctttcta
     6421 gtgaggaaaa attgcatcat gccgaacgca aaggggcgat tttgacctac atcgaagggc
     6481 tggatttagc gctctcactg ctgccgttgg tacacgagaa agcaatcagc atcaacaccg
     6541 ctgccgcagg gggaaatgcc agcttaatga gtgaaatgga ttaagcaaca aacaaaagag
     6601 tataagaggg cagatcacaa tgatctgcct ttttatttta aggggctgtc ctagataacg
     6661 actaaaactt ccatttaggt taagataacc ctatgagaaa aagtcgtcta agtcagtaca
     6721 aacaacataa actcatagag ctgtttgtag caggcgtaat cgctcgaaca gccgcagagt
     6781 tagtgaatgt aaataaaacc accgcagcct attattttca ccgtttgcga ttgctcatct
     6841 atcaacatag ccctcatttg gaaatgtttg agggagaaat tgagattgat gagagctatt
     6901 tcggcggtca tcgcaagggt aaacgaggtc gtggtgcagc aggcaaaacc gccgtattcg
     6961 ggcttttaaa gcgtgacggc aaggtttata ccgtagtagt gccgaacaca caatctgcta
     7021 cacttttgcc gattattcgg gaaaaggtga agcccgatag ccttgtttac accgattttt
     7081 atcgaagcta tgatgtgctt gatgtgagtg aatttaacca ttttcgtatc aatcacagca
     7141 cacacttcgc agaaaatca
//
LOCUS       DANLYI010000125         7156 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.126, whole genome shotgun sequence.
ACCESSION   DANLYI010000125 DANLYI010000000
VERSION     DANLYI010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 7156)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7156)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7156
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.126"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            60..965
                     /locus_tag="PXD81_001773"
     CDS             60..965
                     /locus_tag="PXD81_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HDL5537780.1"
                     /translation="MSQKYSVAVIGLGAMGMGAAKSCIAAGLDTYGVDLNPNYLETLK
                     AAGAKKVGTSAVDFAAELDAVLLLVVNANQVNAVLFGENGLAQHLKQGTVVMVSSTIS
                     AQDAKNISQKLTDLGLVMLDAPVSGGAAKAAQGEMTVMASGSSEAFEKLKPVLDAVAG
                     KVYNIGEEVGLGATVKIIHQLLAGVHIAAGAEAMALAARAGIPLDLMYDVVTNAAGNS
                     WMFENRMKHVVEGDYTPLSMVDIFVKDLGLVNDTAKSLRFPLHLASTAYSMFTEASNA
                     GYGKEDDSAVIKIFSGIELPKKEGK"
     gene            967..2208
                     /locus_tag="PXD81_001774"
     CDS             967..2208
                     /locus_tag="PXD81_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005754893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HDL5537781.1"
                     /translation="MLGIIADDFTGASDIASFLTENGLKTVQMNGVPKTTLNSQVDAI
                     VISLKSRSNPVEEAIQQSLQALDWLKQNGCSQFYFKYCSTFDSTEKGNIGPVTDALMA
                     ALDTDFTVVTPALPVNGRTIFNGYLFVGDVLLNESGMRNHPITPMKDANLVRLMDAQS
                     QGKTGLVAYADVIQGAERVKARFAELKQQGYKYAVVDAADNSQLAVLAEAVADFQLVT
                     GGSGLGAYMAAYRSGGKKGTNAFVPSKGKTVVLSGSCSVMTNTQVAAYQQKAPSYFLE
                     AEKAIQDPNYVETLYQWVIANLDGDFAPMVYATVPPEKLKEIQTQFGGDKASHAIENT
                     FAKLAEKLQAAGVTNFITAGGETSSIVVQQLGFSGFHIGKQIAPGVPWLQAVEKPIFL
                     ALKSGNFGKEDFFSFAQEMFV"
     gene            2205..2837
                     /locus_tag="PXD81_001775"
     CDS             2205..2837
                     /locus_tag="PXD81_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014668373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HDL5537782.1"
                     /translation="MTEQEQKIEMVNLARSLFERGYTVGGAGNLSVRLDENRILVTPT
                     GSSLGRLEADRLSVLDMQGNVLSGDKPSKESVFHLAMYQKNPACQAIVHLHSTYLTAL
                     SCLENLDTDNAMRAFTPYYVMRVGKLPVIPYYRPGDINIARELGERALDGKAFLLANH
                     GVVVTGSDLRDAVDNTEELEETSKLYFILQGQKVRYLTDDEVNDLKNRGK"
     gene            2839..3615
                     /locus_tag="PXD81_001776"
     CDS             2839..3615
                     /locus_tag="PXD81_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005547735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase family protein"
                     /protein_id="HDL5537783.1"
                     /translation="MPKFAANLTMMFNEVPFLDRFEAAANAGFKYVEYLWPYDYPAAE
                     LKAKLEQYGLKQVLFNTAAGDVAQGEWGVSAIPGREADSHRDIDLALEYALALGCENV
                     HVMAAVVPEGEDREAYKQTFIKNVQYAADKFKPHGINVLLEALSPEVKPNYLLKSQFD
                     TLEIVEAVNRDNVFVQLDYFHAQNVDGNLSRLTDKLKGKFAHIQIASVPDRHEPDEGE
                     INYQYIFNKLDEIGYEGYVGCEYKPRAETTSGLGWFEQYK"
     gene            3668..5026
                     /locus_tag="PXD81_001777"
     CDS             3668..5026
                     /locus_tag="PXD81_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntT protein"
                     /protein_id="HDL5537784.1"
                     /translation="MSNEMLILIGLASVVALLVIMIKGKVHPFLALGLVSIAVALTAG
                     IPMSKVIPTLISGMGSTLGSVALIVGLGAMLGKIIEKSHGADVLANWLLQRFGEKNAP
                     FALGMTGFIFGIPVFVDVGFIVLLPIIFSVARRLGGNILVYSLPIGLAMLLVHVLVPP
                     HPGIVAGAQNLNADIGFVLGIGLVAALPAYLIGQKFIPFFTKNNFVKVVTSTDLLQHQ
                     KDLIKEKGIELPSFGKVLAMISFPLILIMLGTVTATTLPKEHIVREVFSMVGSSPFAL
                     FVAVCLSAYVLGFRYGWTKHKLEEVLESALAPIAGIILITGAGGMFGKVLDASGVGKA
                     LADVLANTGLPLLVLSFILSALLRAAQGSATVATITTTTILAPAAVAAGYNDLQLALL
                     TAAIGAGSMTLSHVNDSLFWVWTKFFNVPVSVGLRTWTFLTTIFGVIAFAVVGIIWML
                     FV"
     gene            5088..6428
                     /locus_tag="PXD81_001778"
     CDS             5088..6428
                     /locus_tag="PXD81_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase"
                     /protein_id="HDL5537785.1"
                     /translation="MLKKTLLFTLLLSHSLYAEDMIQENKSANITLLAEITAQGQKIN
                     GVALEYPDNVLSGSDLRQLYQVKTALDSAEKENRSVLKAYVNNEPKVTALPQAGKFVI
                     IELDTADKNAATYSVKDANKAPMKFKSKDENGNIVETEKVQAVKIPEFFANRLIYSVE
                     QKGLLKLTSGTTLGKTEWTQTAEPDKIKTPYLDDFVAKQVSLDQSGNKLNYRLYSPLQ
                     KNGEKYPLVIFLHGSGQVGSDNLAHLLSSKGAIATLPYAPSFVLAPQYDQVFDPFDNA
                     AKGQKGGIHWQTENRINLVLKMIDETLKANPQIDTTRIYINGLSRGAEGGLNLLLARP
                     NFFAGALLMSGREAYTNEWVDGNATKTNLAAIKHIPIWFFHSKEDKVSPVKGSRINYR
                     ILKDELNAPNVKYTEFTFEQAGDNGIVNNNPHNTWDAVYNSPEVIGWLLQQKRH"
     gene            6440..7129
                     /locus_tag="PXD81_001779"
     CDS             6440..7129
                     /locus_tag="PXD81_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005577085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HDL5537786.1"
                     /translation="MFMYLSHPLDPKDLAWPGEPVVEVTRCTDVGEETPFSSFITKLP
                     NHCGTHMDAPRHFVKDGLSINELPIEYFCHKEVALIEIPKGEAEGITKEDLLPYVDIL
                     SKVSFAFLRTGFEKYRTENPAIYQNECPYISTGAGQYLSDTFPNLKGVGIDFLALGSP
                     SSRVPDAENPKNCHRAILGYYTGRFTTVIEDMHLAELPKNANIKQFFNAPWRIKGLDS
                     SQVTCIVELQQ"
BASE COUNT         2058 a         1459 c         1655 g         1984 t
ORIGIN      
        1 cttttctatt ggttaatata ccgccatcaa gttcattaat ttatttgagg gaataaacaa
       61 tgagtcaaaa atatagcgta gccgtcatcg gcttaggagc aatgggtatg ggagcagcaa
      121 aatcttgtat cgctgccgga ttagacacat acggtgtgga tcttaacccg aattatctgg
      181 aaaccttaaa agcagcaggt gctaagaaag tcggcacaag tgcggtcgat tttgccgctg
      241 aattagatgc ggtgttgttg ttggtggtta atgccaacca agtgaatgcg gtgttattcg
      301 gtgaaaacgg cttagcccaa catttgaaac aaggtacggt ggtgatggta tcttccacca
      361 tttctgcaca agatgcaaaa aatatttcac agaagttaac agatttaggc ttggtgatgt
      421 tagatgctcc ggtttcgggt ggggcagcaa aagcagcaca aggtgaaatg actgtgatgg
      481 catcaggttc cagcgaggca ttcgagaaat taaaaccggt attagatgcg gttgcaggca
      541 aagtgtataa cattggcgaa gaagtcggct tgggtgcaac ggtaaaaatt atccatcaat
      601 tacttgccgg tgtgcatatt gccgcaggtg cagaagcaat ggcattggcg gctcgtgccg
      661 gtattccgct tgatttaatg tatgacgttg taaccaatgc ggcaggcaat tcttggatgt
      721 ttgaaaaccg aatgaaacac gttgttgagg gcgattacac cccactctct atggtggata
      781 tttttgtgaa agatttggga ttggtgaacg acaccgcaaa atctttgcgt ttcccgcttc
      841 acttagccag caccgcttat tctatgttta ccgaagccag caacgcaggt tacggcaagg
      901 aagatgatag tgcggtcatt aaaattttca gtggtatcga attacctaaa aaagagggga
      961 aataagatgt taggcattat tgcagacgat ttcaccggtg cgagtgacat cgccagtttc
     1021 ttaaccgaaa acgggctaaa aacggtgcaa atgaatggtg tgccgaaaac tacacttaac
     1081 agtcaagttg atgcgattgt gattagcctg aaatctcgct ctaaccctgt ggaagaagca
     1141 atccaacaat cattacaagc cttagattgg ctgaaacaaa atggttgctc gcaattctac
     1201 tttaaatatt gttccacttt tgacagcacg gagaaaggca atatcgggcc ggtaaccgat
     1261 gccctaatgg cggcattaga taccgatttt accgtagtca caccggctct gcctgtcaat
     1321 ggcagaacca ttttcaacgg ctatttgttt gtgggcgatg tgctactgaa tgaatctggt
     1381 atgcgtaacc acccgattac cccgatgaaa gatgccaact tggtgcgttt aatggatgca
     1441 caatctcaag gcaaaaccgg cttggttgcc tatgctgatg tgattcaagg ggcagagcgt
     1501 gtgaaagccc gttttgctga attgaaacag caaggctaca aatatgcggt agtagatgct
     1561 gccgataatt cacaacttgc ggtgcttgcc gaagcggttg ccgattttca attagtcaca
     1621 ggcggttcag gcttaggggc ttatatggcg gcttaccgtt caggcggcaa aaaaggcacg
     1681 aatgcgtttg taccaagcaa aggcaaaacc gttgtgcttt cgggttcttg ctcggtaatg
     1741 accaataccc aagtggcggc ttaccagcaa aaagccccaa gttatttctt agaagcggaa
     1801 aaagccattc aggatccgaa ctatgtagaa acgctttatc aatgggtgat tgcaaaccta
     1861 gacggtgact ttgccccaat ggtttatgcc accgttcccc ctgaaaaatt gaaagagatt
     1921 caaacccaat tcggcggcga taaagccagc cacgccattg aaaatacctt tgccaaatta
     1981 gccgaaaaat tacaagccgc aggcgtaact aactttatta ccgcaggcgg tgaaacttcg
     2041 agcattgtgg ttcagcaact tggattcagc ggtttccaca tcggcaaaca aattgcaccg
     2101 ggcgtgccgt ggttacaagc ggtcgaaaaa ccgatttttc ttgcattgaa atcgggtaac
     2161 ttcggtaaag aagatttctt cagttttgct caggagatgt ttgtatgaca gaacaagaac
     2221 aaaagattga aatggtcaat cttgcccgct cgctgtttga gcggggctat accgttggcg
     2281 gtgcgggcaa cctctccgtg cgtttagatg aaaatcgcat tttagttacc ccaaccggct
     2341 cttcgctcgg gcgtttagag gcagaccgcc tttccgtatt ggatatgcag ggtaatgttc
     2401 tttcaggaga taaaccttca aaagaatcgg tattccactt ggcgatgtat cagaaaaatc
     2461 cggcttgcca agcaatagtg catctgcatt ccacttattt aaccgcactt tcttgcttgg
     2521 aaaatttgga taccgacaac gctatgcgtg ctttcacccc ttactatgtg atgcgggtag
     2581 gaaaattgcc ggtcattcct tactaccgtc cgggcgacat caacattgcc cgagagctgg
     2641 gggaacgagc cttagacggc aaagcctttt tactggcaaa tcacggggtg gtagtcacag
     2701 gttcagactt gcgtgatgcg gtggataaca cagaagagtt ggaagaaacc tccaagctct
     2761 attttattct acaaggtcaa aaagtccgtt atctcaccga tgacgaagtc aatgatctga
     2821 aaaacagagg aaaataaaat gcctaaattt gcagcaaacc ttacgatgat gtttaacgaa
     2881 gtgccgtttt tagatcgttt tgaagcggct gccaacgctg gatttaaata tgtcgagtat
     2941 ttatggcctt atgattatcc ggcagcagaa ttgaaagcca aacttgagca atatggctta
     3001 aaacaggtgt tgttcaatac cgcagccgga gatgttgctc aaggtgaatg gggtgtttct
     3061 gccattccgg ggcgtgaagc tgatagccat cgtgatattg atttagcgtt ggaatatgcc
     3121 ctcgccttag gttgtgagaa tgttcacgtt atggcagcgg tcgtgccaga aggcgaagat
     3181 cgtgaagcct ataaacaaac ctttatcaaa aacgtgcaat atgccgccga taaattcaaa
     3241 ccgcacggca ttaacgtctt gcttgaagcc ctaagccctg aagtgaaacc gaattatctg
     3301 cttaaaagcc agtttgatac gctagaaatt gtggaagcgg taaaccgaga caatgtgttt
     3361 gtacagcttg actacttcca cgcacagaat gtggacggca acttatctcg cctaaccgac
     3421 aaactcaagg gtaaatttgc ccacattcaa attgcctctg tgccagatcg ccacgaaccg
     3481 gacgaagggg aaattaacta ccaatatatc ttcaacaaac tcgatgaaat cgggtatgaa
     3541 ggctatgtag gttgcgagta taaaccacga gcggaaacca cctcaggact aggttggttt
     3601 gagcagtata aataaccacg atgattagcg gataaatacg tgaatacaac atttagaagg
     3661 aaactttatg tcaaatgaaa tgttaatttt gattggatta gcaagcgtag ttgctcttct
     3721 ggtcattatg attaaaggta aagtacaccc attcttagca ttggggttag tgagtatcgc
     3781 agttgcctta actgcaggta ttccaatgag taaagtgatc ccaaccttga tttcaggtat
     3841 gggaagtact ttaggtagcg ttgcactcat agtaggtttg ggagcaatgt tagggaaaat
     3901 cattgaaaaa tctcacggtg cggatgtatt agcaaattgg ttgttacagc gttttggtga
     3961 gaaaaacgct ccctttgcat taggtatgac aggttttata ttcggtattc ctgttttcgt
     4021 tgatgttggt tttatcgtat tgctcccgat tatttttagt gtagcgagac gtttaggggg
     4081 aaatatcctt gtttattctt tgccaatcgg gcttgcgatg ttacttgttc acgttttagt
     4141 tccacctcat ccaggtattg tagcgggtgc tcaaaactta aacgcagata ttggctttgt
     4201 attgggaatt ggcttagtag cagcgctacc tgcatattta atcggtcaaa aatttattcc
     4261 gttctttaca aaaaataatt tcgtaaaagt agtaacttca accgatcttt tacagcacca
     4321 aaaagatttg attaaagaga aaggcattga actaccttct tttggtaaag tgttagcaat
     4381 gatttctttc ccgctaatcc tgattatgtt aggtacagta acggcaacaa ccttgccgaa
     4441 agaacatatc gtgcgtgaag tattcagtat ggtaggaagc tctccgtttg ccttatttgt
     4501 ggctgtttgt ctttctgctt atgtattagg ttttagatac ggttggacta agcataaatt
     4561 agaagaagta ctggagtctg cgttagcacc gattgcgggg attattttaa tcaccggtgc
     4621 aggcggtatg ttcggtaaag tgttagatgc cagtggcgta ggtaaagcat tagcagatgt
     4681 attagccaac acgggcttac cgttattggt tctttctttc atactttctg cattattgcg
     4741 tgcagcacaa ggttctgcaa cggtggcaac tattactact acaactattc tagctcctgc
     4801 agctgtagca gcaggttata atgatctgca attagcactt cttactgcag ctattggtgc
     4861 aggttctatg acattatccc acgtgaatga ttcactcttc tgggtatgga cgaaattctt
     4921 caatgttcct gtatccgtag gcttaagaac ttggacgttt ttaacgacta ttttcggtgt
     4981 aatagccttt gccgttgttg gcattatttg gatgttattc gtttaatatc actctgttac
     5041 tgcttaaaat ccacctctta tgaggtggat ttttttagga gaaacctatg ctgaaaaaaa
     5101 cacttttatt tacgctatta ctttctcact ccctctatgc agaggatatg attcaggaaa
     5161 ataaatcagc gaatattacg ttgcttgccg aaatcaccgc tcaaggtcaa aaaattaacg
     5221 gcgtggcatt ggaatatcct gataatgtct tatcaggcag cgatcttcgc caactgtatc
     5281 aggtcaaaac ggcattagat agtgcggaga aagaaaaccg ttcggtgctc aaagcctatg
     5341 tcaataatga gccaaaagtg accgcacttc cgcaagccgg taaatttgtg attattgaat
     5401 tggatacggc agataaaaat gcggcaactt atagcgtaaa agatgctaat aaagcgccga
     5461 tgaaatttaa atccaaagat gaaaacggca acattgtaga aacagagaaa gtacaagcgg
     5521 tcaaaattcc agagtttttt gcaaatcgcc taatctattc ggtggaacaa aaaggcttgc
     5581 ttaaattaac cagcggcaca actctaggca agactgaatg gacacaaacc gctgaacccg
     5641 acaaaatcaa aaccccttat ctcgatgatt ttgtcgcaaa acaagtgagc ttagaccagt
     5701 ctggaaataa actgaactac cgtctctatt cgcctttgca aaaaaatggt gaaaaatacc
     5761 cgcttgtgat tttcctacac ggttcagggc aagtcggttc ggataacctt gctcacctgc
     5821 tttccagtaa aggggcgatt gccaccctgc cttatgcccc aagttttgta cttgctccgc
     5881 aatacgacca agtattcgac ccatttgata atgccgccaa agggcaaaaa ggcggtattc
     5941 actggcaaac ggaaaatcgt atcaatctcg tgttaaaaat gattgatgaa accctaaaag
     6001 ccaatcctca aattgatacc acacgcattt atatcaatgg cttatctcga ggtgctgaag
     6061 ggggcttaaa tctgttgttg gctcgcccga atttctttgc cggagcatta ttaatgagcg
     6121 gtcgtgaagc ctacaccaac gaatgggtgg acggcaatgc caccaaaacg aatttggcgg
     6181 caattaaaca tataccgatt tggtttttcc atagtaaaga ggacaaagtc tcgccggtaa
     6241 aaggttctcg cattaactac cgcattctca aagatgagtt aaatgccccc aatgtgaaat
     6301 ataccgaatt cacttttgaa caagccggcg acaacggcat tgtcaataat aaccctcata
     6361 atacttggga cgctgtgtat aacagccctg aggtgattgg gtggttactt cagcaaaaac
     6421 gccactaagg aggctcactg tgtttatgta tttgtctcac ccgttagatc ctaaagattt
     6481 agcttggccg ggagaacctg ttgttgaggt gactcgctgc acagacgtag gtgaagaaac
     6541 gccatttagc agttttatca cgaaactacc gaatcattgc ggcacacata tggacgctcc
     6601 tcgccacttt gtgaaagacg gtttaagtat caatgaattg ccgatagagt atttctgcca
     6661 taaagaggtc gctttaattg aaattcccaa aggagaggca gaagggatca ccaaagaaga
     6721 tttattgcct tatgtcgata ttctctccaa ggtgtctttt gcctttttac gaacggggtt
     6781 tgagaaatat agaaccgaga accctgctat ttatcaaaat gaatgcccct atatttcaac
     6841 cggtgccggg cagtatttat ctgatacttt cccgaatttg aaaggagtag gcattgattt
     6901 cttggcacta gggtctcctt cttcccgagt gcctgatgcg gaaaacccta aaaattgcca
     6961 tcgagcgatt ttaggctact ataccggcag gtttaccact gtgattgaag atatgcactt
     7021 agcggaactc cctaaaaatg ccaacattaa gcagtttttt aatgcaccgt ggcgaattaa
     7081 agggttagat tccagtcaag tgacttgtat tgtggaatta cagcaataaa ccacagatat
     7141 attcatttta ggagag
//
LOCUS       DANLYI010000126         7138 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.127, whole genome shotgun sequence.
ACCESSION   DANLYI010000126 DANLYI010000000
VERSION     DANLYI010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 7138)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7138)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7138
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.127"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            complement(<1..729)
                     /locus_tag="PXD81_001780"
     CDS             complement(<1..729)
                     /locus_tag="PXD81_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL5537787.1"
                     /translation="MAEILFRSCLCCNAKRIFYLLTFYKGFETSQIATAFSLLVFTSQ
                     AFSLFAGSWGDSFGRYPIMMLGCLLDALAYILLLTTKNYPLLLLATFFFGLGSTLFST
                     NARAFLLANIQDTGDSYQLKTKAQGKFLKVSSLSAMVAPLLTIPFIFYRKAEWLIWIS
                     CCIEIIMLFVMFKTAPKTHECANGQCERFKFGALKGILNEQFIFVHLLLFIPLGLGSA
                     FYIIFPYIFTNLLDKPELVSISFFV"
     gene            812..1273
                     /locus_tag="PXD81_001781"
     CDS             812..1273
                     /locus_tag="PXD81_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HDL5537788.1"
                     /translation="MRKIKLLHVILSLILGLSAAAIGSYPAIKEIGEERAKKHQDILD
                     LNRELYSAMVNRDFSALQTLLSDDFELYSSTGEVIDKQEWIKLIQKRVLEFDKVDLLS
                     TDFQGNQSSGLLRVNGEFFGIERDNVVVEMNIRTIVRNTERQIKCIVIKEV"
     gene            1286..2179
                     /gene="murQ"
                     /locus_tag="PXD81_001782"
     CDS             1286..2179
                     /gene="murQ"
                     /locus_tag="PXD81_001782"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HDL5537789.1"
                     /translation="MNALGQMITEQRNPNSMNIDRLSALDIVQVINQEDKQVAIAVEQ
                     CLPQIAQAVEKIVQAFEKGGRLVYVGAGTSGRLGVLDASECPPTYGVKPEMVVGLIAG
                     GERALRFPIEGAEDNREQGKVDLQAVNFSEKDVLVGIAASGRTPYVLGAMEYAKQLGA
                     TTVSIASNPNSAMAQIADIAIETVVGPEVLTGSSRMKSGTAQKLVLNMLTTASMILIG
                     KCYQNLMVDVQATNEKLKARAIRIVMQATECEREVAEDTLKLAENNAKLAIMMILGNL
                     DKQKAEDLLSQNQGRLQKALK"
     gene            complement(2263..3207)
                     /gene="prmB"
                     /locus_tag="PXD81_001783"
     CDS             complement(2263..3207)
                     /gene="prmB"
                     /locus_tag="PXD81_001783"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HDL5537790.1"
                     /translation="MFKYNTELLDEISALPIVNDLVTIQDMMRWGYSYFNASDLYYGH
                     GYDNAWDEAQQLVLAGLYLPFDVPETLYSSRLTRVEKERIINLICERLGSRKPVAYLT
                     NSAWFCGGEYYVDERVIVPRSPIGELIKKGFKGIIKNEPHRILDMCTGSGCIAIACAE
                     RFPEAEVDAVDLSLDALDVAQINIERHQVSHRVFPICSDLFNNLPQDKYDLIVTNPPY
                     VDEEDLGDMPEEFHHEPALALGSGKDGLDLTKRILAQAADYLNDDGVLVCEVGNSMVH
                     LIEQFPSVPFNWLEFEHGGLGVFSLTKEELVKHRHLFA"
     gene            3300..3845
                     /gene="smrB"
                     /locus_tag="PXD81_001784"
     CDS             3300..3845
                     /gene="smrB"
                     /locus_tag="PXD81_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HDL5537791.1"
                     /translation="MNDTLLDDEFALFREAAKGTKKLTQNTFVPKAEPRKKLQELREL
                     KEKADTEFYFSDEYEPLLKEENEKVKYLREDVDPYILKQLRRGDFQPELFLDLHGLTR
                     EKAKKELAALILACEREKVYCASVMTGFGTRTLKDQIPRWLVQHPKVMALHQAPKEWG
                     GDASILILVEQPENPEKMFGR"
     gene            3909..4394
                     /locus_tag="PXD81_001785"
     CDS             3909..4394
                     /locus_tag="PXD81_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HDL5537792.1"
                     /translation="MSEQTFHQLTALLDSHRARYRVVGHPSAGKSEEVAKIRGTEIGQ
                     GAKALVCKVKGNGVKQAVLAILPADYQADLTKIAEHLGGLRASLASPAEVAELTDCVF
                     GAIPPFSFHPDLLLVAEPSLFERYAELAFNAGTLERSIILNTEDYQKIVNPVLVQFAK
                     S"
     gene            complement(4396..5145)
                     /locus_tag="PXD81_001786"
     CDS             complement(4396..5145)
                     /locus_tag="PXD81_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HDL5537793.1"
                     /translation="MEKRFEINEQGLSIRCKLFYEKELQQIENVVIVLHGFGSSKDLK
                     SNTKFGERLSSKYKGYAALAFDFPCHGDDARKKLSVEESITYLQLVIDYAKTSLNAKN
                     LYAYGTSFGGYLTLKYLAEKGNPFRKIALRAPAVQIYHSLMNRLSEEERQKLAKGREI
                     LWGFDRQMKISQAFFDELTDITQNDYLDYAEQILILHGTEDEMVALSTSQQFADNNVI
                     ELLPIEGADHAFSNPKLMDIAIGKIVEFFAP"
     gene            5317..6180
                     /locus_tag="PXD81_001787"
     CDS             5317..6180
                     /locus_tag="PXD81_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA/RhaT family transporter"
                     /protein_id="HDL5537794.1"
                     /translation="MLQLILAILCSVTVGVLIKFARQKGIRIDQSIAVNYIVTTTMTF
                     LLLKPDYQGQSITDIIANNPTSYIFVILSITLPTIFLVQAKALEFAGIIRTDAAQRLS
                     LFLPILAAFTIFGEQVTQNKLIALLLAFVALGCLLWKGNQGMEKGGKIAIISLALVWV
                     GYGVNDILFKQIAKSGSAFPVTLSIAFIGAGCFMFIYLLLKRTQWHVPSVLTGLLLGV
                     LNFGNILFYIQAHQAMKDDPTLVFTGMNLGVICLGTFVGAFFFKEKIHKVNYLGVAIA
                     IVAIICLFYWR"
     gene            6304..6897
                     /locus_tag="PXD81_001788"
     CDS             6304..6897
                     /locus_tag="PXD81_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5537795.1"
                     /translation="MKLKALSTLIASSMLYSQSFAVPPEKTAEEPQPFFNKTESGYDL
                     VFNDQNYRLIETELNGEKIQFRAFERIVYVNNPIEPDYQTINLYVPEAYYNNGEINGY
                     QADTAPIFLPNSVGGYMPAKAASYDKKRRDGSPGTILVALSKGYVVASVGARGRTLQK
                     EGKYTGKAPSAIIDSKSAAHKACRFESDHWHHSLCKI"
BASE COUNT         2210 a         1340 c         1563 g         2025 t
ORIGIN      
        1 cacaaagaat gagatagaga ctaattctgg tttatccaga agattggtga atatataggg
       61 gaatataata taaaatgcac ttcctagacc aagcggaata aaaagtaata aatgtacaaa
      121 aataaattgt tcatttagta ttccttttaa tgcaccaaat ttaaagcgtt cgcattgccc
      181 atttgcacat tcatgtgttt tgggtgcggt tttaaacata acgaatagca taataatttc
      241 aatgcagcag ctaatccaaa taagccattc tgctttacgg tagaaaataa atggaatagt
      301 aagtaatggt gctaccatcg ctgacaggct tgataccttc agaaatttac cttgagcctt
      361 agttttgagt tggtaactat ctccggtatc ttgaatattg gctaataaga atgctctcgc
      421 attggtacta aataaggtgc taccgagccc gaagaaaaag gtagcaagta atagcagagg
      481 gtaatttttt gttgtcagta ataaaatgta agctagggca tcgagcaaac agccaagcat
      541 catgataggg taacgtccaa aactatcacc ccaagaacct gcaaaaaggg aaaatgcttg
      601 gctggtaaag accaacaaag aaaatgcagt tgcaatttgg ctggtttcaa agcctttgta
      661 gaatgtaagt agataaaaaa tacgctttgc attgcagcat aagcaagacc ggaaaagaat
      721 ttccgccata aaatgatatt ttggatattc atagataata tttaatgaga aaaatgattt
      781 ctaatataac taaatttaat aaggaagcac aatgagaaaa atcaaattat tgcatgttat
      841 tttatcttta atattagggc tttctgcggc agcgattgga agttatccgg caataaaaga
      901 gattggcgag gagcgggcaa aaaaacatca agatatatta gatttaaata gagaacttta
      961 tagtgcaatg gtgaaccgtg atttttcggc tttgcaaaca ttactaagcg atgattttga
     1021 gctttattca tcaacaggtg aagtgattga taagcaagaa tggatcaaat taatccaaaa
     1081 gagagtattg gaatttgata aggttgattt gctatctacc gattttcagg ggaatcaatc
     1141 atctggatta ttaagagtaa atggtgagtt ttttgggata gagcgggata atgtggtggt
     1201 agaaatgaat attcgaacca ttgtgagaaa tacagaacgc caaattaaat gtattgtgat
     1261 taaagaggtt taagtgtcgc aactattgaa tgccttaggg cagatgatta ccgaacaacg
     1321 taatccgaat tcgatgaata ttgaccgcct ttcagcgtta gatatagtcc aagtcatcaa
     1381 ccaagaagac aaacaagtag cgattgcggt ggagcagtgc ctgcctcaaa ttgcacaagc
     1441 ggtggaaaaa attgtgcaag cctttgagaa aggtggacga ttggtttatg tcggtgccgg
     1501 cacgagcgga agattagggg tgcttgatgc ttcggaatgt ccgcccactt atggcgtaaa
     1561 acctgaaatg gtggttggat taattgccgg tggtgagcgg gcattacgtt tcccgattga
     1621 aggggcggaa gataatcgtg agcaaggcaa agtagattta caagcggtca attttagcga
     1681 aaaagatgta ttggtcggga ttgcagccag tgggcgaacg ccttatgtgt taggggcgat
     1741 ggaatatgct aagcaacttg gtgcgaccac ggtgtcgatt gccagtaacc caaattcggc
     1801 aatggcacaa attgcggata ttgcgattga aaccgttgtc gggccggaag tgctaaccgg
     1861 ttccagccgt atgaaatccg gcacagctca aaaattggta ttgaatatgc tcactactgc
     1921 gtcgatgatt ttaatcggta aatgctacca aaatttaatg gtagatgtgc aagcaactaa
     1981 tgaaaaactc aaagctagag caattcggat tgtgatgcaa gcgacagaat gtgaacgaga
     2041 agtggctgaa gatacactaa aattagccga aaataacgct aaattggcaa ttatgatgat
     2101 tttaggtaac ttagataagc agaaagccga agatttatta agccaaaatc aagggcgatt
     2161 acaaaaagcg ttaaaataga agtatgtctg ctctcccctt gtgggagaga gacagatcaa
     2221 tcattcattc agaatgattg atcagagaga ggggaggcag atttaagcaa ataaatggcg
     2281 atgtttcact agttcttctt tggttaaact gaacacccct aagccaccgt gttcaaattc
     2341 cagccagtta aacggcacac tggggaattg ttcgattaaa tgcaccatac tgttgccgac
     2401 ttcgcagact agcacgccat catcatttaa gtaatcagcg gcttgagcaa gaatccgctt
     2461 ggttaaatct aacccatctt tgcccgagcc aagagctaat gccggctcgt gatggaactc
     2521 ttcgggcata tcgcctaagt cttcttcatc aacataaggc ggattagtca caattaagtc
     2581 gtatttatct tgcggtaaat tgttaaacag atcggaacag atcgggaaaa ctcggtgaga
     2641 cacctgatga cgctcaatat taatttgagc aacgtctaag gcatcaaggg aaagatcaac
     2701 cgcatcgact tctgcttccg gaaaacgctc ggcacaggca atcgcaatac aaccgctgcc
     2761 ggtacacata tctaaaattc gatgtggctc gtttttgata atgcctttaa accctttttt
     2821 gatcagctca ccaatcggtg aacgtggcac gatcacacgc tcatcgacat aatattcacc
     2881 gccacaaaac caagccgagt tggtaaggta tgctacaggt ttgcgagagc ctaaacgctc
     2941 acagattaaa ttgatgatac gttctttctc aactcgagtt aagcgagagg aataaagtgt
     3001 ttccggtaca tcaaacggca aataaagccc tgccaacact aattgttgtg cctcgtccca
     3061 cgcattatcg tagccgtgcc cgtaataaag atcagaggca ttaaaatagc tataccccca
     3121 acgcatcata tcttgaatag tgactaaatc attcacaatg ggcagggcgg aaatttcatc
     3181 aagaagttcg gtattatatt taaacattgg cgtgcctttt ttcgtattgt taaaatggct
     3241 gtgttatact acattttgct ttgtgttaga atgtaaaaat gaataagaag gaaaagataa
     3301 tgaacgatac gttattagac gatgaatttg cattattcag agaggcagca aaaggcacga
     3361 aaaagctgac acaaaatacc tttgtgccga aagcggaacc tcgcaagaaa ttgcaagaat
     3421 tgcgagaatt aaaagaaaaa gctgatacag aattttattt ctcggatgaa tatgagcctt
     3481 tgctgaaaga agaaaatgaa aaagtgaaat atctgcgaga agatgttgat ccttatattc
     3541 tcaaacagtt acggcgaggc gatttccaac ccgagctgtt tttagatctg catggtttaa
     3601 ctcgagaaaa agcgaagaaa gagttggcag cgttgatttt agcctgtgag cgggaaaagg
     3661 tatattgtgc cagtgtgatg accggctttg gcactcgcac gctcaaagat caaattccac
     3721 gctggttggt tcagcaccca aaagtaatgg cgttgcacca ggccccgaaa gagtggggcg
     3781 gagatgcctc tattctaatt ttggttgagc agcctgaaaa tcctgaaaaa atgtttgggc
     3841 gttaaaattc ggctgaattg ccaatttttt attaaaaacg accgcttgtg tatcattaag
     3901 gagaaatgat gtcagaacaa actttccacc aacttacagc attattagat tcgcatcgag
     3961 ctcgctatcg agtggtagga catccttctg ccgggaaatc ggaagaagtg gcaaaaattc
     4021 gaggaacaga aatcgggcaa ggggcgaaag ccttagtttg caaggtaaaa ggcaatgggg
     4081 taaagcaagc tgtgcttgct atattacctg ctgattacca agcggattta acaaaaattg
     4141 cagagcatct tggtgggtta agagcctcac ttgccagccc ggctgaagtg gcagaattga
     4201 ccgattgtgt atttggggca attccgccgt ttagctttca ccccgattta ttattggtgg
     4261 ctgagccaag tctgtttgag cgttatgctg aattagcctt taatgccggt acgttggaac
     4321 gttcgattat tctgaatacc gaggattacc aaaagatagt taatcctgtg ttggttcaat
     4381 tcgccaaaag ctaggttatg gggcgaaaaa ctccacgatt ttgccaatag cgatatccat
     4441 taatttaggg ttagaaaaag cgtgatctgc cccttcaatc gggagcagtt caatcacgtt
     4501 attatcggca aactgctgtg aagtgcttaa cgccaccatt tcatcttccg tgccgtgcag
     4561 aattaaaatt tgctcggcat aatccaaata atcgttttgg gtaatatcgg ttagctcatc
     4621 aaaaaaggct tggctgattt tcatctggcg atcaaacccc cacaaaattt ctcgcccttt
     4681 ggcaagtttt tgccgttcct cttctgacaa gcggttcatt aacgaatggt agatttgtac
     4741 tgccggtgcc cgcaatgcaa ttttccgaaa cggattgcct ttttccgcca gatatttcaa
     4801 ggttaaatag ccgccaaaac tagtgccgta ggcatacaga tttttagcat tcaggctggt
     4861 tttggcataa tcaatcacca gttgcagata agtaatggat tcctcaaccg agagtttttt
     4921 acgggcatca tcaccgtggc aagggaaatc aaaggcaagg gcagcgtagc ctttatattt
     4981 tgagctaagg cgttccccaa atttggtatt cgatttcaga tctttagacg aaccaaagcc
     5041 gtgcaacaca atcactacat tctcgatctg ctgcaattct ttttcataaa acagtttaca
     5101 gcgaatgctg agaccctgtt cattaatctc aaagcgtttt tccattttat gttaagtatt
     5161 gaaattgaag atgacaaaat tttagcacta tttgagtgca agcggtcgga tctctcgcaa
     5221 aatttgcaaa ttttccggta aatttaaccg cttgtactag ccaaaattta agcatattta
     5281 tagaatacag ccttcgtttt taaaaggaga aatgaaatgt tacaacttat tttagcgatt
     5341 ttatgtagcg tgactgtagg cgtgttgatt aaattcgccc gccaaaaagg gattcgtatc
     5401 gaccaaagta ttgcggtgaa ctatatcgtg actaccacga tgacgttttt attactaaaa
     5461 ccggattacc aagggcagtc gattaccgat attattgcta ataacccgac ttcctatatt
     5521 tttgtgattt taagtatcac attgcctact attttcttgg tgcaagcaaa agccctagag
     5581 tttgccggta ttatccgaac cgatgcggca caacgtttat cgctgttcct gccgattttg
     5641 gcggcattca ccatttttgg tgagcaggtt acgcaaaata aattaattgc acttctgtta
     5701 gctttcgtgg cactcggttg cttattatgg aaaggcaatc agggaatgga aaaaggcgga
     5761 aaaattgcga ttatcagcct tgctctggtt tgggtaggct atggcgtgaa cgatatttta
     5821 tttaaacaga ttgccaaatc aggctctgct ttccctgtca ccctttcgat tgcctttatc
     5881 ggtgcaggct gttttatgtt tatctatttg ctacttaaac gcactcaatg gcacgttcca
     5941 agtgtcttaa ccggcttatt gctaggcgtg ttgaacttcg gcaatattct gttctatatt
     6001 caagctcacc aagccatgaa agatgaccca actttagtct tcactggaat gaacttaggg
     6061 gtaatttgct taggcacttt cgttggagca ttcttcttta aagagaaaat ccacaaagtg
     6121 aactacttag gcgtggcgat tgcgattgtg gcgattattt gcttgtttta ttggcggtaa
     6181 atatactttc caaaattaag ggcgaacata atgttcgccc ttaatttttg agtaatatag
     6241 tgtaaaatat tcaataaatt cgaccgcttg ttgtaccgat tcatatttca taaaggacaa
     6301 aaaatgaagc taaaagcctt atctacactc attgcctctt caatgctata tagtcagagt
     6361 tttgctgtac cgccggaaaa aacagctgaa gagccacagc cctttttcaa taaaaccgaa
     6421 tctgggtatg atttagtctt taatgatcaa aactatcgtt taattgaaac ggaattaaat
     6481 ggcgagaaaa tccaattccg tgcctttgag cggattgtgt atgtgaacaa tccgattgag
     6541 ccagattacc aaacaattaa tctgtatgtg ccggaagctt actacaataa tggcgaaatc
     6601 aatggctacc aagcggatac cgccccgatc ttcttgccaa attcagtggg gggctatatg
     6661 ccggcaaaag cggcaagtta tgataaaaaa agacgtgacg gttcaccagg tacgattttg
     6721 gtggcgttat ctaaaggcta tgtggtggca agtgtgggag caagaggacg cacgctacaa
     6781 aaagagggaa aatacaccgg caaagcacct tctgccatta ttgattcaaa atccgccgcc
     6841 cataaagcgt gtcggttcga gtccgaccac tggcaccact cactatgtaa aatttagttt
     6901 tacatagttt gctgtaagca tttgaatttt gaatcgtttg tgtaggattg ttcaaatcat
     6961 attcgcaaat acacagttac tacgatgtat ccagccaata catccattgg gattttccgt
     7021 ccgaatttca gacgttctag atcttacaca ttctttcaag tttgtaaagc ctgaaaatca
     7081 ttttttagat tgattttatt tgaaaaaatc tattttttct tttaattcaa atttagtc
//
LOCUS       DANLYI010000127         7070 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.128, whole genome shotgun sequence.
ACCESSION   DANLYI010000127 DANLYI010000000
VERSION     DANLYI010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 7070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7070
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.128"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            <1..475
                     /locus_tag="PXD81_001789"
     CDS             <1..475
                     /locus_tag="PXD81_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="toxin-activating lysine-acyltransferase"
                     /protein_id="HDL5537796.1"
                     /translation="NITWLWMNSSLHKEWSCELLARNVIPAIENEQYMLLIDNGIPIA
                     YCSWADLNLETEVKYIKDINSLTPEEWQSGDRRWIIDWVAPFGHSQLLYKKMCQKYPD
                     MIVRSIRFYPKQKELGKIAYFKGGKLDKKTAKKRFDTYQEELATALKNEFNFIKK"
     gene            491..3352
                     /locus_tag="PXD81_001790"
     CDS             491..3352
                     /locus_tag="PXD81_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RTX family hemolysin"
                     /protein_id="HDL5537797.1"
                     /translation="MGTRLTTLSNGLKNTLTATKSGLHKAGQSLTQAGSSLKTGAKKI
                     ILYIPQNYQYDTEQGNGLQDLVKAAEELGIEVQREERNNIATAQTSLGTIQTAIGLTE
                     RGIVLSAPQIDKLLQKTKAGQALGSAESIVQNANKAKTVLSGIQSILGSVLAGMDLDE
                     ALQNNSNQHALAKAGLELTNSLIENIANSVKTLDEFGEQISQFGSKLQNIKGLGTLGD
                     KLKNIGGLDKAGLGLDVISGLLSGATAALVLADKNASTAKKVGAGFELANQVVGNITK
                     AVSSYILAQRVAAGLSSTGPVAALIASTVSLAISPLAFAGIADKFNHAKSLESYAERF
                     KKLGYDGDNLLAEYQRGTGTIDASVTAINTALAAIAGGVSAAAAGSVIASPIALLVSG
                     ITGVISTILQYSKQAMFEHVANKIHNKIVEWEKNNHGKNYFENGYDARYLANLQDNMK
                     FLLNLNKELQAERVIAITQQQWDNNIGDLAGISRLGEKVLSGKAYVDAFEEGKHIKAD
                     KLVQLDSANGIIDVSNSGKAKTQHILFRTPLLTPGTEHRERVQTGKYEYITKLNINRV
                     DSWKITDGAASSTFDLTNVVQRIGIELDNAGNVTKTKETKIIAKLGEGDDNVFVGSGT
                     TEIDGGEGYDRVHYSRGNYGALTIDATKETEQGSYTVNRFVETGKALHEVTSTHTALV
                     GNREEKIEYRHSNNQHHAGYYTKDTLKAVEEIIGTSHNDIFKGSKFNDAFNGGDGVDT
                     IDGNDGNDRLFGGKGDDILDGGNGDDFIDGGKGNDLLHGGKGDDIFVHRKGDGNDIIT
                     DSDGNDKLSFSDSNLKDLTFEKVKHNLVITNSKKEKVTIQNWFREADFAKEVPNYKAT
                     KDEKIEEIIGQNGERITSKQVDDLIAKGNGKITQDELSKVVDNYELLKHSKNVTNSLD
                     KLISSVSAFTSSNDSRNVLVAPTSMLDQSLSSLQFARAA"
     gene            3427..5553
                     /locus_tag="PXD81_001791"
     CDS             3427..5553
                     /locus_tag="PXD81_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_063101322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I secretion system permease/ATPase"
                     /protein_id="HDL5537798.1"
                     /translation="MEANHQRNDLGLVALTMLAQYHNISLNPEEIKHKFDLDGKGLSL
                     TAWLLAAKSLALKAKHIKKEISRLHLVNLPALVWQDNGKHFLLVKVDTDNNRYLTYNL
                     EQDAPQILSQDEFEACYQGQLILVTSRASVVGQLAKFDFTWFIPAVIKYRKIFLETLI
                     VSIFLQIFALITPLFFQVVMDKVLVHRGFSTLNIITVALAIVIIFEIVLSGLRTYVFS
                     HSTSRIDVELGAKLFRHLLSLPISYFENRRVGDTVARVRELDQIRNFLTGQALTSVLD
                     LLFSFIFFAVMWYYSPKLTLVILGSLPCYILWSIFISPILRRRLDEKFARSADNQAFL
                     VESVTAINMIKAMAVAPQMTDTWDKQLASYVSSSFRVTVLATIGQQGVQLIQKTVMVI
                     NLWLGAHLVISGDLSIGQLIAFNMLSGQVIAPVIRLAQLWQDFQQVGISVTRLGDVLN
                     SPTEQYQGKLSLPEIKGDISFKNIRFRYKPDAPTILNNVNLEIRQGEVIGIVGRSGSG
                     KSTLTKLLQRFYIPENGQVLIDGHDLALADPNWLRRQIGVVLQDNVLLNRSIRENIAL
                     SDPGMPMERVIYAAKLAGAHDFISELREGYNTIVGEQGAGLSGGQRQRIAIARALVNN
                     PKILIFDEATSALDYESEHIIMQNMQKICQGRTVILIAHRLSTVKNADRIIVMEKGEI
                     VEQGKHHELLQNSNGLYSYLHQLQLN"
     gene            5565..7001
                     /locus_tag="PXD81_001792"
     CDS             5565..7001
                     /locus_tag="PXD81_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_140441352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family type I secretion periplasmic adaptor
                     subunit"
                     /protein_id="HDL5537799.1"
                     /translation="MKIWLSGIYEFFLRYKNIWAEVWKIRKELDHPNRKKDESEFLPA
                     HLELIETPVSKKPRLIAYLIMLFLVVAIVLASVSKVEIVATAPGKLTFSGRSKEIKPI
                     ENAIVQEIFVKDGQFVEKGQLLVSLTALGSDADIKKTMASLSLAKLENYRYQTLLTAI
                     EKESLPVIDLSRTEFKDSSEEDRLRIKHLIEEQYTTWQKQKTQKTLAYKRKEAEKQTI
                     FAYVRKYEGATRIEQEKLKDFKALYKQKSLSKHELLAQENKLIEAQNELAVYRSKLNE
                     LENDLLNVKEELELITQFFKSDVLEKLKQHIENERQLRLELEKNNQRRQASMIRAPVS
                     GTVQQLKIHTIGGVVTTAETLMIIVPEDDVLEATALVPNKDIGFVAAGQEVIIKVETF
                     PYTRYGYLTGRIKHISPDAIEQPNVGLVFNATIAIDRKNLTSPDGRKIDLSSGMTITA
                     EIKTGERSVMSYLLSPLEESVTESLRER"
BASE COUNT         2351 a         1241 c         1454 g         2024 t
ORIGIN      
        1 aaacattact tggctatgga tgaactcctc cctccacaaa gaatggagct gtgaactact
       61 agcacgcaat gtgattcctg caattgaaaa tgaacaatat atgctactta tagataacgg
      121 tattccgatc gcttattgta gttgggcaga tttaaacctt gagactgagg tgaaatatat
      181 taaggatatt aattcgttaa caccagaaga atggcagtct ggtgacagac gctggattat
      241 tgattgggta gcaccattcg gacattctca attactttat aaaaaaatgt gtcagaaata
      301 ccctgatatg atcgtcagat ctatacgctt ttatccaaag cagaaagaat taggcaaaat
      361 tgcctacttt aaaggaggta aattagataa aaaaacagca aaaaaacgtt ttgatacata
      421 tcaagaagag ctggcaacag cacttaaaaa tgaatttaat tttattaaaa aatagaagga
      481 gacatccctt atgggaacta gacttacaac cctatcaaat gggctaaaaa acactttaac
      541 ggcaaccaaa agtggcttac ataaagccgg tcaatcatta acccaagccg gcagttcttt
      601 aaaaactggg gcaaaaaaaa ttatcctcta tattccccaa aattaccaat atgatactga
      661 acaaggtaat ggtttacagg atttagtcaa agcggccgaa gagttgggga ttgaggtaca
      721 aagagaagaa cgcaataata ttgcaacagc tcaaaccagt ttaggcacga ttcaaaccgc
      781 tattggctta actgagcgtg gcattgtgtt atccgctcca caaattgata aattgctaca
      841 gaaaactaaa gcaggccaag cattaggttc tgccgaaagc attgtacaaa atgcaaataa
      901 agccaaaact gtattatctg gcattcaatc tattttaggc tcagtattgg ctggaatgga
      961 tttagatgag gccttacaga ataacagcaa ccaacatgct cttgctaaag ctggcttgga
     1021 gctaacaaat tcattaattg aaaatattgc taattcagta aaaacacttg acgaatttgg
     1081 tgagcaaatt agtcaatttg gttcaaaact acaaaatatc aaaggcttag ggactttagg
     1141 agacaaactc aaaaatatcg gtggacttga taaagctggc cttggtttag atgttatctc
     1201 agggctatta tcgggcgcaa cagctgcact tgtacttgca gataaaaatg cttcaacagc
     1261 taaaaaagtg ggtgcgggtt ttgaattggc aaaccaagtt gttggtaata ttaccaaagc
     1321 cgtttcttct tacattttag cccaacgtgt tgcagcaggt ttatcttcaa ctgggcctgt
     1381 ggctgcttta attgcttcta ctgtttctct tgcgattagc ccattagcat ttgccggtat
     1441 tgccgataaa tttaatcatg caaaaagttt agagagttat gccgaacgct ttaaaaaatt
     1501 aggctatgac ggagataatt tattagcaga atatcagcgg ggaacaggga ctattgatgc
     1561 atcggttact gcaattaata ccgcattggc cgctattgct ggtggtgtgt ctgctgctgc
     1621 agccggctcg gttattgctt caccgattgc cttattagta tctgggatta ccggtgtaat
     1681 ttctacgatt ctgcaatatt ctaaacaagc aatgtttgag cacgttgcaa ataaaattca
     1741 taacaaaatt gtagaatggg aaaaaaataa tcacggtaag aactactttg aaaatggtta
     1801 cgatgcccgt tatcttgcga atttacaaga taatatgaaa ttcttactga acttaaacaa
     1861 agagttacag gcagaacgtg tcatcgctat tactcagcag caatgggata acaacattgg
     1921 tgatttagct ggtattagcc gtttaggtga aaaagtcctt agtggtaaag cctatgtgga
     1981 tgcgtttgaa gaaggcaaac acattaaagc cgataaatta gtacagttgg attcggcaaa
     2041 cggtattatt gatgtgagta attcgggtaa agcgaaaact cagcatatct tattcagaac
     2101 gccattattg acgccgggaa cagagcatcg tgaacgcgta caaacaggta aatatgaata
     2161 tattaccaag ctcaatatta accgtgtaga tagctggaaa attacagatg gtgcagcaag
     2221 ttctaccttt gatttaacta acgttgttca gcgtattggt attgaattag acaatgctgg
     2281 aaatgtaact aaaaccaaag aaacaaaaat tattgccaaa cttggtgaag gtgatgacaa
     2341 cgtatttgtt ggttctggta cgacggaaat tgatggcggt gaaggttacg accgagttca
     2401 ctatagccgt ggaaactatg gtgctttaac tattgatgca accaaagaga ccgagcaagg
     2461 tagttatacc gtaaatcgtt tcgtagaaac cggtaaagca ctacacgaag tgacttcaac
     2521 ccataccgca ttagtgggca accgtgaaga aaaaatagaa tatcgtcata gcaataacca
     2581 gcaccatgcc ggttattaca ccaaagatac cttgaaagct gttgaagaaa ttatcggtac
     2641 atcacataac gatatcttta aaggtagtaa gttcaatgat gcctttaacg gtggtgatgg
     2701 tgtcgatact attgacggta acgacggcaa tgaccgctta tttggtggta aaggcgatga
     2761 tattctcgat ggtggaaatg gtgatgattt tatcgatggc ggtaaaggca acgacctatt
     2821 acacggtggc aagggcgatg atattttcgt tcaccgtaaa ggcgatggta atgatattat
     2881 taccgattct gacggcaatg ataaattatc attctctgat tcgaacttaa aagatttaac
     2941 atttgaaaaa gttaaacata atcttgtcat cacgaatagc aaaaaagaga aagtgaccat
     3001 tcaaaactgg ttccgagagg ctgattttgc taaagaagtg cctaattata aagcaactaa
     3061 agatgagaaa atcgaagaaa tcatcggtca aaatggcgag cggatcacct caaagcaagt
     3121 tgatgatctt atcgcaaaag gtaacggcaa aattacccaa gatgagctat caaaagttgt
     3181 tgataactat gaattgctca aacatagcaa aaatgtgaca aacagcttag ataagttaat
     3241 ctcatctgta agtgcattta cctcgtctaa tgattcgaga aatgtattag tggctccaac
     3301 ttcaatgttg gatcaaagtt tatcttctct tcaatttgct agagcagctt aatttttaat
     3361 gattggcaac tctatattgt ttcacacatt atagagttgc cgttttattt tataaaagga
     3421 gacaatatgg aagctaacca tcaaaggaat gatcttggtt tagttgccct cactatgttg
     3481 gcacaatacc ataatatttc gcttaatccg gaagaaataa aacataaatt tgatcttgac
     3541 ggaaaagggc tttctttaac tgcttggctt ttagctgcaa aatcgttagc gttgaaagcg
     3601 aaacacatta aaaaagagat ttcccgctta cacttggtga atttaccggc attagtttgg
     3661 caagataacg gtaaacattt tttattggta aaagtggata ccgataataa ccgctattta
     3721 acttacaatt tggaacaaga tgctccacaa attctgtcac aagacgaatt tgaagcctgc
     3781 tatcaagggc agttaatttt ggtcacgtcc agagcttccg tagtaggtca attagcaaag
     3841 ttcgatttca cctggtttat tccggcggtg atcaaatacc gaaaaatctt tctagaaacc
     3901 ttgattgttt cgatcttttt gcaaattttt gccctaatta caccgctatt cttccaagtt
     3961 gttatggata aagtactggt gcatcgaggt ttttcaacct tgaatatcat tacggttgcc
     4021 ttagctattg tgatcatctt tgaaattgta ctaagtggtt tgagaaccta tgttttttct
     4081 catagcacta gccgtattga tgttgaatta ggcgctaaat tatttcgaca tttattatca
     4141 ctacccattt cttattttga aaacagacga gttggagata cagtcgctag ggttagagaa
     4201 ttagatcaaa ttcgtaattt ccttaccgga caagcattaa cctcggtgtt agatctctta
     4261 ttctctttta tcttttttgc cgtaatgtgg tattacagcc caaaattaac cttggtaatt
     4321 cttggttcat tgccctgcta tattttatgg tcaattttta ttagtccgat tttaagacgg
     4381 cgtttagatg agaaatttgc ccgaagtgct gataaccaag cattcttagt tgagtcggta
     4441 acagccatca atatgattaa agcgatggcg gttgctccac aaatgacgga tacatgggat
     4501 aaacagctgg caagctatgt ttcatcaagt ttccgtgtca ccgtattagc aaccattggg
     4561 caacaaggtg tacaacttat tcaaaaaacc gttatggtga ttaacctttg gttaggggca
     4621 cacttagtta tttcaggcga tctgagtatt gggcaattaa ttgcctttaa tatgctatca
     4681 gggcaagtga ttgcaccggt gattcggctg gctcagctct ggcaagattt ccaacaagtt
     4741 gggatttccg tcactcgctt aggtgatgtt ttaaactctc caaccgaaca atatcaaggc
     4801 aaattatcac taccagaaat aaaaggcgat atctcattta aaaatatccg ctttagatat
     4861 aaaccagatg caccaactat tttaaataat gtgaatttag aaattaggca aggagaagtg
     4921 attgggattg ttggacgttc cggttcaggc aaaagtactc tgactaaatt actgcaacgt
     4981 ttttatattc ctgaaaatgg gcaggttttg attgatggac atgatctagc cttagctgat
     5041 ccaaactggc tacgccgtca aataggtgta gtgctgcaag ataatgtgtt attaaaccgc
     5101 agtatccgag aaaatattgc gctatcagat ccaggaatgc caatggagcg agtaatttat
     5161 gcagcaaaat tagcaggggc tcacgatttt atttcagaat tgcgtgaagg ttataacacc
     5221 attgtgggtg aacaaggagc ggggctttca ggcgggcaac gccaacggat tgcgattgct
     5281 cgagctttgg taaacaaccc gaaaatcctg atttttgatg aggcaaccag tgccctcgat
     5341 tacgaatctg agcatattat tatgcaaaat atgcaaaaaa tatgccaagg cagaaccgtg
     5401 attttgattg cacatcgttt atcgaccgtc aaaaatgcgg atcgaattat tgtgatggaa
     5461 aagggggaaa ttgttgagca aggcaagcac cacgaattac tgcaaaacag taacggactt
     5521 tattcctact tacaccaatt acaacttaat taagaaggaa aacaatgaaa atatggctta
     5581 gtggtattta tgaatttttc ctacgctata aaaacatttg ggcagaagta tggaaaattc
     5641 gtaaagaatt agaccaccca aacagaaaaa aagacgaaag tgaattttta ccggcacatt
     5701 tagaactgat tgaaaccccg gtttctaaaa aaccacgtct aattgcttat ttgattatgc
     5761 tatttttagt tgtggcaatt gtgcttgcca gtgtaagcaa agttgaaatt gtggcgactg
     5821 ctcccggtaa attaactttt agtggcagaa gtaaagaaat taaaccgatt gaaaacgcca
     5881 ttgtacaaga aattttcgtt aaagatgggc agtttgtgga aaaagggcaa ttattagtca
     5941 gcttaactgc attgggttct gatgcagata tcaaaaagac catggcttca ctttctttag
     6001 ctaaactgga gaactatcgc taccaaactt tgcttactgc cattgaaaaa gagtccttgc
     6061 cggtgattga tttatctaga accgaattta aagattcatc ggaagaagat cgactacgta
     6121 ttaaacactt aattgaggag caatacacca cttggcaaaa acaaaaaaca cagaaaactt
     6181 tagcgtataa gcgtaaagag gctgaaaaac aaacaatatt tgcctatgtc cgtaaatatg
     6241 aaggtgcaac acgtattgaa caagaaaaat taaaagactt taaggcactt tataaacaga
     6301 agtctttatc taagcacgaa cttcttgcgc aagaaaataa attaattgag gctcagaatg
     6361 agctagctgt ttatcgctca aaattaaatg aattagaaaa tgatctactc aatgtaaaag
     6421 aagaacttga attgatcacg caattcttta aaagcgatgt gttggaaaaa ttaaagcaac
     6481 atattgaaaa tgaacgccaa cttcggctcg agttagaaaa aaataatcaa cgcagacagg
     6541 cctcgatgat cagagcaccg gtttccggta cggttcagca actgaaaatt cacactatag
     6601 gtggtgttgt tacgactgct gaaaccttga tgatcattgt gccggaagac gatgtgttag
     6661 aggccaccgc tctggttcca aacaaagata tcggctttgt tgcagcaggg caggaggtga
     6721 ttattaaagt ggaaactttc ccttatacac gctatggtta tctaactggt cgaattaaac
     6781 atattagccc ggatgcgatt gaacaaccta atgtaggctt agtttttaat gcaactatag
     6841 ctatagatag gaagaatcta acatcgcctg atgggcgaaa aattgatttg agttcaggta
     6901 tgacaataac tgctgaaatc aaaaccggtg aacggagtgt aatgagttat ttactcagcc
     6961 cattagaaga atctgtcaca gaaagtttaa gggaacgcta atcgaaccaa aacaaagcca
     7021 taaaagccat tttgagcttt tatggcttta ttttttagtc cacaagcggt
//
LOCUS       DANLYI010000128         7026 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.129, whole genome shotgun sequence.
ACCESSION   DANLYI010000128 DANLYI010000000
VERSION     DANLYI010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 7026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7026
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.129"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            79..939
                     /gene="araC"
                     /locus_tag="PXD81_001793"
     CDS             79..939
                     /gene="araC"
                     /locus_tag="PXD81_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HDL5537800.1"
                     /translation="MYKNLSLLSPTYPFDLEFIAGLTQCEKGNYLDSIVDRQQGMHGY
                     MLQLTTFGRGSVFDGKHVFTAHRGQLLLFTPNAVQHYHRHPESQYWHYKWVYFQPNPK
                     WNKWLNWSDMEEKIGRITISDDRYFQEISQLFSKIELELKSSKFFKEDIATSLLEYLL
                     MKCISAEKIEVIPVIDQRILTVCDLILTNLAKNESLEAMAEKVFLSPSRLSHLFKQSL
                     GISLVQWRELQRISESKKLLYFSNISISNIAKSLGYEDSLYFSKIFKKHTGLSPSEFR
                     TLERRKDSEM"
     gene            1126..2100
                     /locus_tag="PXD81_001794"
     CDS             1126..2100
                     /locus_tag="PXD81_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HDL5537801.1"
                     /translation="MKKPTFKHALFSSILALACYGSAFANDVVNISKIDGMPWFNRMA
                     EGVVQAGKDNNINAYQVGPSNTDAPQQVKLIEDLIAKKVSAISIVPNDANALEPVLKK
                     AKQNGIVVLTNESVGQPSADWDIEIIDNAQFAADYVEEVAKAMGGKGGYVIYVGGLTV
                     PQHNLWADLFVKYQKEHYPDMFEVTSRMPVAENINDARRTTIDLVKAHSDLKAVVSFG
                     SQGPIGAGLAVKEKRLKNKLNVFGMMIPSQAANLIKSGDITMGITYDPANAGYALAAV
                     AAKVLKGEKIEDGLEIPTVGKAKVDSEKKLLQFHNVLKVTKENIDKLY"
     gene            2155..3645
                     /locus_tag="PXD81_001795"
     CDS             2155..3645
                     /locus_tag="PXD81_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HDL5537802.1"
                     /translation="MAFISMRHISKTFHGIKALNQVNLSLDYGEALCLAGQNGCGKST
                     LIKILSGVYQPDKGAEIQIGATQYTKLTPQESIDKGIQVIYQDLALFPNLTVAENIAI
                     NLHRKFGWVSQDHIHQVALTAIQSINANLDLNAILEDLPIAQQQLVAICRALAQNARL
                     LIMDEPTASLTAKEVKDLLDVVIKLKSKGISIIFVSHKLQEVMSVSDSVLVLKDGNMV
                     GQYPIREMNEKRLGFLMTGLEIDYKRLDLPDFLQRKTVLEVRNLTLPHQYQEINFSLR
                     EGEIVALTGLLGSGRTELCLSLFGITQPQSGDILLNGEKVIFNTNRDAIAKGIAYVSE
                     DRMHTGLIMAESIHHNIISTIFHKITDKLQIIQSSKAYASSQALIDSLKIKVSDSDLP
                     VNTLSGGNAQRVSIAKWLAIDPKIIILDAPTIGVDIANKEGIFQIIKSLAQQGIAVIF
                     VTDEVEETYYNSHRVIVMKKGRIVDEILPAYSSEKSIAEVIYENQQ"
     gene            3629..4588
                     /locus_tag="PXD81_001796"
     CDS             3629..4588
                     /locus_tag="PXD81_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL5537803.1"
                     /translation="MKISNNHILSSTLLILILLLTLFTRDFATIENLYDVVNNYAMLM
                     ILACGLFVVLLSGGIDISFPAITIISQYVMVTLIGKYDVGFAGVFVISSTIGILLGFI
                     NALFVNRLSVPSIIITISTLNIFYGGLLYFTKGVWLYEYPEWFTQEIVFFKQITAEGV
                     EFGLSFQALMAILAVILTGLITNKLTIGRKIYALGGNPDATSRIGFNLLFLHLFAYGY
                     MGFMAGLAGIVQSYTVQSVAPDSLLGYELTVLAAVVLGGASLSGGRGTLLGTVMGVML
                     LAIIQNGLNLMNVSSYWQTIITGIIIILSISATALSQRKKQGA"
     gene            4591..5541
                     /locus_tag="PXD81_001797"
     CDS             4591..5541
                     /locus_tag="PXD81_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL5537804.1"
                     /translation="MKSKLDPTIFYLLALFAVAVIAFSIFIPDIFWSVSNFQSMASQI
                     PVLGILTLAMALPMLTGGINLSIIAGMNACSLIIAYFITQYTGISWLFIAILLSLCCA
                     LAIGLLNGTLIAIIRVSPILATLGTMTLINGINILVTNGSTIANFPETMLSINASAFL
                     WIPLPILIFLFLAGLIWFFLEKTAMGKAVYFIGNNEKATYYSGINTKKVLIWVYVISS
                     ILCVFAAILMMSKLNSAKASYGDSYLLISILAAVLGGINPDGGKGKLLGIMLALVLLQ
                     VIESGLNMLGISSYITMILWGSLLLFFIFLQKNRLFRLTQ"
     gene            5554..>7026
                     /locus_tag="PXD81_001798"
     CDS             5554..>7026
                     /locus_tag="PXD81_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015587080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HDL5537805.1"
                     /translation="MQNEKQLIEQGNTVIGIELGSTRIKAVLISSDGTILATGGADWE
                     NRLIDGIWTYHQNEIWEKLQAAYMDLSKTVKEKYHTTIRTAKALGISAMMHGYLPFDK
                     HGNQLVAFRTWRNNITLTSSEKLTALFQYPIPQRWSIAHLHQALLNQEPHLPEIDYLT
                     TLSGYVHWQLTDHKVLGIGDASGMFPIDTELKSYNPTMLAQFDRAIADYRMPWKLEAI
                     LPQVRVAGEQAGKLTEKGAKLLDPTDNLQAGIPLCPPEGDAGTGMIATNCIKEKTGNI
                     SAGTSAFAMIVLEKALSKAYSELDIVTTPAGKLVAMAHANNCTSDINAWVNLFGECLS
                     VFGVNICTEELYEMLFLHALQGDADCGGLLSYGFHSGEHNVGLSEGCPVFLHPTNAHF
                     NLANFMRTHLYTAFGAMKLGMDILINQEKVEISRILGHGGIFKTEGVAQKILSSALNI
                     PLATASTASNGGAWGIALLADFLSVSDRYSLEEYLDNCIFN"
BASE COUNT         2093 a         1349 c         1405 g         2179 t
ORIGIN      
        1 actaatctga gcgttcattc attagtaatg ataaacaagc ggttattttt tgttattttt
       61 tggtaaatac tgatagtcat gtataaaaac ctttctttgc tttcaccgac ttatccattt
      121 gaccttgaat tcatcgccgg tcttactcaa tgtgagaaag gtaactatct tgattctatt
      181 gtcgataggc aacaaggaat gcacggttat atgcttcaat taaccacttt tggtcgtggt
      241 tctgtttttg atggtaagca cgtttttacc gctcatcgag ggcagttact cctctttaca
      301 cccaatgccg ttcaacacta ccaccgtcac ccggaaagcc aatattggca ctacaaatgg
      361 gtctattttc agccgaatcc taaatggaat aaatggctga attggtcaga tatggaagaa
      421 aaaataggac gtatcaccat tagcgatgat cgttactttc aggaaattag ccaacttttc
      481 agtaaaattg aattagaact taaatccagt aaatttttta aagaagatat tgcaacaagt
      541 ttgttggaat acttacttat gaaatgtatt tccgccgaga aaattgaggt tattcctgtc
      601 attgatcaac gcattttgac agtttgtgat ttaatcttaa cgaatttggc taaaaatgag
      661 agcttagaag caatggcaga aaaagtattt ttatcgccat ctcgcctctc acatcttttc
      721 aagcaatcgc tggggatcag cttagtgcaa tggcgggaat tacaacgcat ttccgaatcc
      781 aaaaaattgc tttatttctc taacatctct atctctaata ttgcgaaatc cttaggctat
      841 gaggattctc tctacttttc caaaattttc aaaaagcata ccgggctgtc accctctgag
      901 tttagaacct tagaacggcg taaagacagt gaaatgtgat cttgatctca ctttactttg
      961 catagcagaa ttttccatat cgataacaaa aagttacctg tttttctctc attcaaaagc
     1021 ataagataca aacagcttta atagtgctgt ttctattaga gaagggatat caagctctgt
     1081 gagtgatatt cagtgaaata acttcttatg tatgagggaa gaacaatgaa aaaaccaacg
     1141 ttcaagcacg cattattttc aagtatttta gctttagcct gttacggctc ggcatttgcc
     1201 aatgatgttg ttaatatctc caaaattgac ggtatgccgt ggtttaatcg tatggcagag
     1261 ggtgttgtgc aagcaggcaa agataacaat atcaatgctt atcaagttgg tccatcaaat
     1321 accgatgcac cacaacaagt aaagttaatt gaagatttaa ttgcgaaaaa agttagtgct
     1381 atttccatcg tccctaacga tgcaaatgcc ttagaaccgg tactgaaaaa agcgaaacag
     1441 aacggtattg tggtattaac caatgagtca gtcggacaac ctagtgccga ttgggatatt
     1501 gaaattatcg ataatgcaca atttgcagca gactatgtag aagaagtagc aaaagctatg
     1561 ggcggaaaag gcggctatgt gatttatgtt ggtggtttaa ccgtgcctca acataatctg
     1621 tgggctgatt tattcgtgaa ataccagaaa gagcattatc cggatatgtt tgaagtgacc
     1681 agcagaatgc ctgttgccga aaatattaat gatgcacgcc gtaccaccat tgatttagtg
     1741 aaagcccatt ccgatcttaa agcggtcgtt tcttttggtt cacaaggtcc tattggtgca
     1801 gggttggcag tgaaagaaaa acgcttaaaa aataaattaa atgtcttcgg aatgatgatt
     1861 ccttcccaag cagccaattt aattaaaagc ggtgatatta caatgggaat tacctatgac
     1921 ccggcaaatg caggttatgc tttagctgct gtcgctgcca aagtattaaa aggcgaaaaa
     1981 attgaggatg gattagagat cccaacggtc ggtaaagcca aagtagatag tgagaaaaaa
     2041 ctacttcaat tccacaatgt gctgaaagtc accaaggaaa atatcgataa attatattaa
     2101 tttgcttgaa atatgggctg atagccagcc catattttcc ctcaggaggc acttatggca
     2161 tttatttcga tgcggcatat cagtaaaaca ttccacggca taaaagcatt aaatcaagtc
     2221 aatctctcat tagattatgg cgaagcactt tgtcttgccg gtcaaaatgg ctgtggaaaa
     2281 tcaaccttga tcaaaattct ttccggcgtt tatcaaccgg ataaaggggc tgaaattcaa
     2341 attggtgcaa cccaatatac caagctgaca ccacaggaat cgattgataa aggtattcaa
     2401 gtgatttatc aagatttagc cctcttcccc aatttaaccg tggcagaaaa tattgccatt
     2461 aacctgcacc gaaaatttgg ctgggtgagt caagatcaca ttcatcaggt tgccctcact
     2521 gctattcaaa gcattaatgc aaatctagac ctgaatgcaa tattagaaga tttaccgatc
     2581 gctcaacaac agttagtcgc tatttgtcga gcgttagcac aaaatgccag attattgatt
     2641 atggacgagc cgactgcctc acttactgca aaagaggtca aagatctgtt agatgtcgtc
     2701 atcaaactta aaagcaaagg cattagcatc atctttgtca gtcataaatt gcaagaagta
     2761 atgagtgttt ccgatagcgt attagtgctg aaagatggca atatggttgg gcaatatcct
     2821 atccgtgaaa tgaatgaaaa acgccttggt ttcctaatga ctgggctgga aatcgattac
     2881 aagcggttag atttgccgga ttttttgcaa agaaaaaccg tattggaagt ccgcaattta
     2941 acgctgcctc atcagtatca agagattaac ttttcgctac gagaagggga aattgttgcc
     3001 cttacaggat tgctcggttc agggcgtact gagctttgcc taagtttatt cggcattact
     3061 caaccacaat ccggcgatat tttattaaac ggtgaaaaag tgatatttaa cactaaccgt
     3121 gatgccattg ccaaagggat tgcttatgta tcggaagata gaatgcacac cgggctgatt
     3181 atggcagagt cgattcatca caatattatt tcgactattt ttcacaaaat caccgataaa
     3241 ttacagatta ttcaatcctc gaaggcttat gcaagtagcc aagccttgat tgattcatta
     3301 aagattaaag tatccgattc agatttgcct gttaataccc tttccggagg gaacgcacag
     3361 cgagtttcga ttgcaaaatg gctggcaatc gaccccaaaa ttattatttt agatgccccg
     3421 actattgggg tcgatatcgc caataaagaa gggatcttcc aaattattaa atcattggct
     3481 cagcaaggca ttgcagttat ttttgtgact gatgaagtgg aagagaccta ctacaacagc
     3541 catcgggtaa ttgtaatgaa aaaaggtcgc attgttgatg aaatcttgcc ggcatattcc
     3601 agtgaaaaat cgattgcgga ggttatttat gaaaatcagc aataatcata ttctttccag
     3661 tacgttactg attttaatcc tattattaac cctatttact cgtgattttg caactattga
     3721 gaacctttat gatgttgtaa ataattacgc aatgttaatg attttagctt gtggtttatt
     3781 tgtggtattg ctgtctggcg gcattgatat ttctttccct gctatcacta ttatttccca
     3841 atatgttatg gtcactctga tcggtaaata tgatgtagga tttgccggcg tttttgttat
     3901 ttcttccacc atcggcatat tattaggttt tattaatgct ctatttgtta atcggcttag
     3961 tgtcccttcc attattatta cgatctcaac actcaatatt ttttatggcg gactactcta
     4021 ttttaccaaa ggtgtatggc tttatgagta tcccgaatgg tttacccaag aaatcgtgtt
     4081 ctttaaacag ataactgctg agggagtgga atttggatta tcgtttcaag cattaatggc
     4141 tattttggcg gtgatattaa ccggcttaat taccaataaa ctcactattg gtcgaaaaat
     4201 ttacgctctt ggtggtaatc cggatgcaac ctcccgtatt gggtttaatc tcttatttct
     4261 ccatctattt gcatacggtt atatgggctt tatggcaggc ttggcaggca tagtgcaatc
     4321 ttataccgta caatctgtcg caccggattc actactgggt tatgaattaa ccgtcctagc
     4381 tgccgttgta ttaggtggtg ctagtttatc aggtggtaga ggcaccctgc ttggtacagt
     4441 aatgggcgta atgttactgg cgattattca aaacgggcta aatttaatga atgtatcgtc
     4501 ttactggcaa accattatta ccggtatcat cattatctta agtattagtg caacagcctt
     4561 aagtcagcgg aaaaaacaag gagcataacg atgaaaagta aactggatcc aacgattttc
     4621 tatctcttag cactcttcgc agttgcagtt atcgccttta gtattttcat tccggatatt
     4681 ttttggtctg tcagtaactt tcaatctatg gcatcacaaa ttccagtatt aggtatttta
     4741 accctagcaa tggcattacc gatgttaaca ggtggaataa atctttccat tattgccgga
     4801 atgaatgctt gctcattaat tattgcctac tttattacgc aatataccgg tatttcttgg
     4861 ttatttattg ctattttatt aagcctttgc tgtgccttag ccattggatt actcaacggc
     4921 acattaattg ccattattcg tgtttcacca attttggcaa ccttaggcac aatgacttta
     4981 attaacggca ttaatattct agtcactaac ggttcaacca ttgcgaactt tccggaaaca
     5041 atgttgagta ttaatgcttc agccttttta tggattccgc tacccattct aatctttctc
     5101 tttcttgctg ggctgatttg gtttttctta gaaaaaactg caatgggtaa agcggtttat
     5161 ttcatcggca ataatgaaaa agccacctat tattcgggta ttaataccaa aaaagtgctg
     5221 atttgggttt atgttatctc ctcaattctt tgtgtatttg cagctatttt gatgatgtct
     5281 aaactcaatt ctgcaaaagc ctcttatggt gattcctatt tgttgatctc cattttagcc
     5341 gcagtattgg gcggtattaa ccctgatgga ggtaaaggga aactattagg gattatgctc
     5401 gccttagttt tattacaagt gattgaaagt ggcttaaata tgctcggcat tagcagttac
     5461 attacaatga ttttatgggg aagcttattg ttattcttta tcttcctcca aaaaaatcgc
     5521 cttttccgac taacacagta atggtggtag attatgcaaa atgaaaaaca actcattgaa
     5581 caaggtaata ctgttattgg tattgaattg ggctcaaccc gaattaaagc ggtgttaatt
     5641 agctcagatg ggacgatttt agcaaccggt ggggcggatt gggaaaatcg actgattgac
     5701 ggtatttgga cctatcatca aaatgaaata tgggaaaaac tacaagctgc ttatatggat
     5761 ttaagcaaga cagtcaaaga aaaatatcac acaaccattc gcacagccaa agcattggga
     5821 attagtgcga tgatgcacgg ctacttacct ttcgataaac acggaaatca gctggttgcc
     5881 tttagaactt ggcgaaacaa cattacccta acctcatcgg aaaaattgac cgcactcttt
     5941 caatacccaa ttccgcaacg ttggagtatt gctcatttac accaagcatt gcttaaccaa
     6001 gagcctcact tgccggaaat tgattacctg acaaccctct caggttatgt gcattggcaa
     6061 ctgaccgatc acaaagtgtt agggattggc gatgcttccg gcatgttccc gatagatact
     6121 gagttgaaat cctacaatcc aacaatgctg gctcaatttg atcgagccat tgccgactac
     6181 cgtatgcctt ggaaattgga agcgattttg ccacaagttc gtgttgctgg tgaacaagcg
     6241 ggcaaattaa ccgaaaaagg tgcaaaattg ttagatccaa ccgataattt gcaagctggc
     6301 attccacttt gtccacctga gggtgatgcc ggaacaggta tgattgcaac caactgtatc
     6361 aaagaaaaaa ccggtaatat ttctgccggc acctccgcat ttgcaatgat tgtgttagaa
     6421 aaagccctat ccaaagccta ttcagagctg gatattgtga cgaccccggc aggtaaatta
     6481 gtggcaatgg ctcacgctaa taattgcacc tctgatatta atgcgtgggt gaatttattc
     6541 ggcgaatgct tatcggtttt tggcgtaaat atctgcaccg aagaacttta tgaaatgtta
     6601 tttctccatg ccttgcaagg cgatgcagat tgtggagggc tattatccta cggcttccac
     6661 tccggtgaac ataatgtcgg tttatccgaa ggctgtccgg ttttcttgca tccaacgaat
     6721 gcccatttta acttagcaaa ctttatgcga acccatctct ataccgcttt tggtgcaatg
     6781 aaattaggaa tggacattct cattaaccaa gaaaaagtgg aaatttcccg tattctcggt
     6841 cacggtggta tatttaaaac agaaggtgtt gcacaaaaaa ttctgtcttc cgcactgaat
     6901 attccattgg ctaccgcaag tactgcttca aatggcggag cttggggaat tgcattactg
     6961 gcagactttc tcagcgtatc agaccgttac agtttagaag aatatttaga taattgcatt
     7021 ttcaac
//
LOCUS       DANLYI010000129         7019 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.130, whole genome shotgun sequence.
ACCESSION   DANLYI010000129 DANLYI010000000
VERSION     DANLYI010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 7019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7019
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.130"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            366..2225
                     /locus_tag="PXD81_001799"
     CDS             366..2225
                     /locus_tag="PXD81_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HDL5537806.1"
                     /translation="MSNLKVKVQGFGRFLSNMVMPNIGAFIAWGFITALFIPTGWLPN
                     ETFAKLVGPMITYLLPLLIGYSGGKLVGGERGAVVGAITTMGIIVGSEIPMFLGAMIV
                     GPLGGWAIKTFDKAIDGKVKSGFEMLVNNFSVGIIGMILAMLSFAFIGGIVTQISAAL
                     GAGVGALVEAGLLPVTSIIVEPAKILFLNNAINHGVFTPLGTAQAQEVGKSILFLIEA
                     NPGPGFGILLAYMFFGRGTAKQTAGSASIIHFLGGIHEIYFPYVLMSPRLILAVIAGG
                     ATGVLTNSLLGGGLIAPASPGSIFAILAMTAPGAHFAVVASVILSCAVTFAIASILLK
                     TQKGEASLEDAQAQTQAMKAESKGVVNGNINKIYVACDAGMGSSAMGASMLRKKVQAA
                     GLNIEVQNLAINDLPQDAQLVITHKDLTARATQRVATAQHLSLTNFLDSAFYDSLVAK
                     LANHQPQQAVQNTQNFANPVEEPQAEGLKLQAAQVFLGLQANSKEEAIRFAGEQLVKA
                     GFVQPSYVDAMFAREQMVSTYLGEGLAVPHGTSDAKDSVLKTGIVVCQYPNGVRFTDE
                     EDGVAKLVVGIAAKGNDHIQILSAITNALDDEQAMKTLTSTNDVEKVLALLSK"
     gene            2329..3468
                     /locus_tag="PXD81_001800"
     CDS             2329..3468
                     /locus_tag="PXD81_001800"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005557909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HDL5537807.1"
                     /translation="MNALHFGAGNIGRGFIGKLLADARIFVTFADINQTQIDQINQNK
                     QYGVKIVGDDSRVEIVKNIAAINSKDENAVIEQVKNTDLITTAVGPNVLGFIAPLFAK
                     ALVARVESGNTQPLNIIACENMVRGTTFFKGKIFEHLTAEQQAEIEKVVGFVDSAVDR
                     IVPPAEPNPADPLEVTVEEFSEWIVDQTQFKGDIPNIKGMELTDNLMAFVERKLFTLN
                     TGHLICAYLGKQAGVKWIKEAIAIEEIKTQVKATMEESGAVLIKRYGFDPQAHSAYIE
                     KILKRFANPYLNDDVNRVGREPIRKLSENDRLIKPLRGTLEYGLPYQNLVKGVVMALQ
                     YRNEEDPQAVELAEFIANHGVAAAVEKYTGLTDQAVIAQVVELYQ"
     gene            3480..3995
                     /locus_tag="PXD81_001801"
     CDS             3480..3995
                     /locus_tag="PXD81_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MltR family transcriptional regulator"
                     /protein_id="HDL5537808.1"
                     /translation="MMDYIDKLSEETTLRGFLSIANQRFAKAVEQLIERVFRKTDFAL
                     KSVVDSLFEHQGPLADIEVRLKVLLGLGVISPKVFEDISLFLEVKQHLNEEEDEFAFS
                     HPAVVKFAKDLHHIDFLPINELLNMKASAANKDSMLFQMQQMRLEKMVRSSLILAVTE
                     IDEQLNVESPL"
     gene            4135..6000
                     /locus_tag="PXD81_001802"
     CDS             4135..6000
                     /locus_tag="PXD81_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012263322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HDL5537809.1"
                     /translation="MQSLLSFVPDFLKQELFWVMALLVFAIWQFIQNKLRMDVVALIV
                     MLFFSLTNILSVKEVLAGLSDPNVVLIALLFIVGEGLVRTGVANQVSDWLMRVSGASE
                     TKVLVLLMLCIAGLGSFMSSTGIVAIFIPVVLAICSGMGISPRRLMMPLSVAGLISGM
                     MTLIATPPNLVAHSELVKSGFEGFNFFSFTPIGLIILVMGIGYMLIARRWLDNGEQSN
                     THSQAQKFSMSQLIEEYQLKERAKMVLVEADSDCVGKSIGQLNLRIEQGLNIIAIQRT
                     KRFRKITVSASVNEIFQPKDILLLDTALDDETYQQRCEQLKLRPIELKGEYFSSQYRS
                     VGMAEISVMPEAECIGKTIRELKFRSRYHLSIVGLKRGDEIIQEELLDTPLKFGDILL
                     VMGVWQQIHRMDDDHRDFFLLRDPAESKNAPPALSQAPHALFSVLTMVLLMITGIVPN
                     VMAALIACLMLGKFRCVDMKSAYDSIHMPSIVLIVGMMPFSIALQKTGGVDLTVRLLL
                     DAMQGLDVHFVLIVLFVVTAIISAFISNTATAILVMPIAIAIANQLGYSPAPFVMTVA
                     VSASAAFMTPVSSPVNTMVLAPAGYKFIDFVKVGVPFTVLVMLVSVFVIPMLFPL"
     gene            complement(6290..6541)
                     /locus_tag="PXD81_001803"
     CDS             complement(6290..6541)
                     /locus_tag="PXD81_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5537810.1"
                     /translation="MDKQAILKLFRDNSTAGNSQPSQEPIQVFNNSNVIIQNGDNATV
                     IAGCTVDNNTNLTCADKQRVHDLIHSLEQRNRASRDEGA"
     gene            complement(6553..>7019)
                     /locus_tag="PXD81_001804"
     CDS             complement(6553..>7019)
                     /locus_tag="PXD81_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL5537811.1"
                     /translation="KVIGFGNRIEQSRKNAKLSRSEVCDALGGIAISTLQAWEADTRE
                     PPISKLMALSQILNVEPCYLLTGERHSQSLNVTREPQPVYATKPQTEEGEVVVIKAKL
                     QEAIETLEEVLEITQREMKPAGKAQMVWAFYELLTQEKSEKEKMVGLLKLVA"
BASE COUNT         1862 a         1453 c         1658 g         2046 t
ORIGIN      
        1 aaactgttgc aaaactaaca aaataggctc cgctcttctc ataaaactta tattttctac
       61 tcattatgaa cctctaaagt gcagtttatg ctgatagtaa tataggtgaa cagttagggt
      121 tagcaattca ttttattttt tcgatgtaga tcgcaaaaat agaattatgc ttggcacaag
      181 cgaggacgct tacgccatct ttactccaaa aatgtgagat agatcacatt ttcaaatctg
      241 cccaaaaaaa tttaatgtga tctacttcac atttctgaga gaaacatttt tgtaactttt
      301 gaaagtggtg ctaatttaag cacgattttt tcaaccctaa ttttctctcc ttttttgagg
      361 tttctatgtc taatttaaaa gtgaaagtcc aaggttttgg gcgtttctta tcgaatatgg
      421 tgatgccgaa tatcggtgcc tttattgcgt ggggctttat caccgccctg tttattccaa
      481 ccggttggtt gccgaacgag acttttgcta aactggtcgg tccgatgatt acttacttgc
      541 tgccactgtt aatcggttac agcggtggta aattagtcgg tggtgagcgt ggtgcggttg
      601 taggtgcgat tactacaatg ggtattatcg tgggttctga aatcccgatg ttcttaggtg
      661 cgatgattgt aggtccatta ggcggctggg cgatcaaaac cttcgataaa gcgattgacg
      721 gcaaagtaaa aagcggtttt gagatgttgg taaacaactt ctcggtcggg atcatcggta
      781 tgattttggc aatgctttca tttgctttca ttggaggtat cgtcacccaa atctctgcgg
      841 cattaggtgc aggtgtgggg gcgttggttg aagctggtct tctgccagta acctctatca
      901 tcgttgaacc ggcgaaaatc ctattcttaa acaatgcgat caatcacggt gtgttcacgc
      961 cattgggtac agcacaagca caagaagtgg gcaaatctat tctgttcttg attgaagcga
     1021 acccgggtcc gggctttggt atcttgcttg cctatatgtt ctttggtcgt ggcacagcaa
     1081 aacaaaccgc tggtagtgcg tcaatcattc acttcttagg cggtattcac gaaatttatt
     1141 tcccgtatgt gttaatgagc ccacgcttaa ttcttgcagt gattgcaggc ggtgcaaccg
     1201 gcgttttaac caactcatta ttaggtggcg gtttaatcgc accggcttcc ccgggttcta
     1261 tttttgcgat tttagcaatg accgcaccgg gtgctcactt tgcagttgtt gcctccgtga
     1321 ttctttcttg tgccgtgact tttgcgattg cgtcaatctt actcaaaacc caaaaaggtg
     1381 aagcctcatt ggaagatgca caagcacaaa ctcaagcgat gaaagcagaa agcaaaggcg
     1441 tagtaaatgg taacatcaac aaaatctatg tagcgtgtga tgccggtatg ggttcaagtg
     1501 cgatgggtgc gagtatgtta cgcaaaaaag tgcaagcggc aggtttaaac attgaagtac
     1561 aaaacttagc gatcaacgac ttaccgcaag acgcacaact ggtgattacc cacaaagact
     1621 taaccgctcg tgcaacacaa cgtgtggcaa cggctcaaca cttatcgctc actaacttct
     1681 tagacagtgc gttctatgac agcttagtgg caaaactggc taaccaccag ccacaacaag
     1741 cggtgcaaaa tacacaaaat tttgcaaacc cggtggaaga acctcaagcc gaagggttaa
     1801 aattgcaagc agcccaagtc tttttaggct tacaagctaa cagtaaagaa gaagcgatcc
     1861 gctttgccgg tgagcagtta gttaaagcgg gctttgttca accaagctat gtagatgcaa
     1921 tgttcgcccg tgagcaaatg gtttcaactt acttaggcga aggcttagct gtgccacacg
     1981 gtacatctga tgccaaagac agcgtactga aaaccggtat cgtggtctgc caatacccga
     2041 acggcgttcg cttcaccgat gaagaagacg gcgtggcgaa actggtggtc ggtattgcag
     2101 caaaaggcaa cgaccatatc caaattttaa gtgcaattac caacgcactg gacgatgaac
     2161 aagcaatgaa aacactgacc tcaacaaatg acgtggaaaa agtgttagca ttattatcta
     2221 aataatcacc ctgttgtgta ggggcgggtc ctgtgcccgc ccattatcct acaaaattta
     2281 gggcgggcac aggacccgcc cctacgtttt atagaggaac aatcatcaat gaacgcactc
     2341 cattttggtg ccggtaatat cgggcgtggc tttatcggca aattgcttgc tgatgctcgc
     2401 attttcgtga cttttgccga tattaaccaa actcaaatcg accaaatcaa ccaaaacaag
     2461 caatacggcg tgaaaatcgt gggcgatgac agccgtgtgg aaattgtcaa aaacattgcg
     2521 gcaattaact cgaaagacga aaatgccgtg attgagcaag tgaaaaacac cgatttaatt
     2581 accaccgcag tcggcccgaa tgtgcttggc tttattgccc cactgttcgc caaagcgtta
     2641 gtggctcgtg tggaaagtgg caacacccaa ccgctgaaca ttatcgcctg tgaaaatatg
     2701 gtgcgtggca caactttctt taaaggtaaa atttttgagc atttaactgc cgagcagcaa
     2761 gcagaaattg aaaaagtggt tggttttgtt gatagtgcgg tggacagaat tgtaccacct
     2821 gccgagccga accctgctga tccgttggaa gtcacggtgg aagagttcag cgagtggatt
     2881 gtggatcaaa cccagttcaa aggtgacatt ccaaacatca aaggaatgga attaaccgat
     2941 aacctgatgg cgtttgtgga acgcaaactg ttcaccctga acaccgggca tttaatctgt
     3001 gcctacttgg gcaaacaagc gggcgtgaaa tggattaaag aggcgattgc gattgaggaa
     3061 atcaaaactc aggtgaaagc cacaatggaa gagagcggtg ctgtgctgat taaacgttac
     3121 ggcttcgacc cacaagccca ttcagcgtat atcgaaaaaa tcctcaaacg ttttgccaac
     3181 ccgtacttaa acgatgacgt aaaccgtgta gggcgtgagc caatccgcaa actgagcgaa
     3241 aacgaccgct tgatcaaacc gttgcgtggc acgttggaat atggcttacc gtatcaaaac
     3301 ttggtcaaag gcgtggtgat ggcattgcaa tatcgcaatg aggaagaccc acaagcggtc
     3361 gaacttgccg aatttattgc aaatcacggt gtggcggcag cggtggaaaa atacaccggt
     3421 ttaaccgacc aagcggtcat tgctcaagtg gtggaattat atcaataatt cttgacacta
     3481 tgatggatta catcgacaaa ctcagtgagg aaaccaccct gcgtggtttc cttagcattg
     3541 ccaatcaacg ctttgccaaa gcggttgaac agttgattga acgagtgttt cgcaaaacgg
     3601 actttgcgtt aaaatccgtg gtggattcct tgtttgaaca tcaaggaccg ctggctgaca
     3661 ttgaagtacg tttaaaagtg ttgcttgggt tgggggtgat ctcacctaaa gtgtttgaag
     3721 atatctcgct tttccttgaa gtcaaacagc atttgaatga ggaagaagac gagttcgcat
     3781 tttctcaccc ggcggtagtg aaatttgcca aagatttgca ccatattgat tttttgccta
     3841 tcaatgaatt gctgaatatg aaagcgagtg cagcgaataa agattcgatg ctctttcaaa
     3901 tgcagcaaat gcggttggaa aaaatggtac gttcaagcct gattttggcg gtaactgaaa
     3961 ttgacgaaca actgaatgtg gaaagcccat tgtaaaaatt tcgtcaaaac taaccgcttg
     4021 tactgagcat tcacgccgta cgtgattgct ttttcctttc tttttgatct tatcggcttt
     4081 tttctttgct taaagcggtg taatctatcc ctaactattt tggaggatat ggttatgcaa
     4141 tcgttgctct cttttgtgcc ggattttctt aagcaagaac tgttttgggt aatggcgtta
     4201 ttggtatttg cgatttggca atttatccaa aacaagttgc gtatggacgt ggttgcctta
     4261 attgtaatgc tgttttttag cctgactaat attttatcag tcaaagaagt gttggcaggc
     4321 ttaagtgacc caaacgtggt gctgattgcg ttgctcttta ttgtcggaga aggtttggtg
     4381 cgaaccggcg tggcgaacca agtcagcgat tggttgatgc gggtgtcggg agcgagcgaa
     4441 accaaagtgc tggtgctgct aatgctctgt attgccgggc ttggatcttt tatgagttcc
     4501 accggcattg tggcgatttt cattccggtg gtgcttgcca tttgctcggg aatgggaatt
     4561 tctcctcgcc ggttaatgat gccgttaagt gttgccggac ttatcagcgg tatgatgacc
     4621 ttaattgcca caccacccaa ccttgttgct cattccgaat tagtaaaatc aggttttgaa
     4681 ggcttcaatt tcttcagttt tactccgatt ggattaatta ttttagtgat gggcatcggc
     4741 tatatgctga ttgcccgccg ttggttggat aatggtgagc aaagcaatac tcacagtcag
     4801 gctcaaaaat tttcaatgtc gcagttaatt gaagagtatc aacttaaaga gcgagccaaa
     4861 atggtgctgg tggaagcaga ttcagactgt gtcggcaaat ctatcgggca actgaattta
     4921 cggattgagc aagggctaaa catcattgca attcaacgca ctaagcggtt tagaaaaatt
     4981 accgtcagtg cctcggtaaa cgaaattttc cagccgaaag atattttact gcttgatact
     5041 gccctcgatg atgaaactta ccaacaacgc tgtgaacagc tcaaacttcg cccgattgag
     5101 ctaaaaggcg aatacttttc ctcacaatat cgctcggtgg gtatggcgga aatttcggtt
     5161 atgcctgaag cagaatgtat cggtaaaact attcgggaac tcaaattccg ctcaagatac
     5221 catttaagca ttgtcggctt aaaacgtggt gatgaaatca tacaagagga actgctcgat
     5281 acgccactca aattcggcga tattttattg gtgatgggcg tatggcaaca aattcaccgt
     5341 atggacgacg atcatcgaga tttcttcttg cttagagatc cggcagaaag taaaaacgcc
     5401 cctccggcgt taagccaagc ccctcacgct ctgttctcag tgctgactat ggtgttgctg
     5461 atgattaccg gcattgtgcc aaacgtgatg gcagcgttga ttgcctgctt aatgttaggc
     5521 aagttccgct gtgtggatat gaaaagtgct tatgattcga ttcatatgcc aagcattgtg
     5581 ttgattgtgg ggatgatgcc gttttctatt gccttacaaa aaaccggtgg tgtggattta
     5641 accgttaggc ttctacttga tgcaatgcaa ggcttagatg tacattttgt tctaatagta
     5701 ttatttgtag taacagcgat tatcagtgct tttatttcca acacagcgac tgcaattttg
     5761 gttatgccga tagccattgc gattgctaac caacttggct actcaccggc accttttgtg
     5821 atgacggtcg ctgtttcagc ctccgccgct tttatgacgc cggtttcttc accggtaaat
     5881 acgatggtat tagcaccggc aggctataaa tttattgatt tcgttaaagt tggtgtgccg
     5941 tttactgtac tggtgatgct ggtgtcggtg tttgtgattc cgatgttatt tccgctctaa
     6001 acactcctct aaattacaag cggtcatttt tggtaatctg acgttgacgg taaaattgtt
     6061 gagtatgtct ggcaacatag tgtggggtac tgatcttttt tgcatagaga gaaagaaaaa
     6121 aatttgggcg gaaaaataac ggcgaggcta ggaaatacaa ggcttcgccg tttttgtttg
     6181 gggtgagtta tgcaaaaaac gaaaatgagc aaaaatagaa aaaaatgggc tatttttacg
     6241 ttattttttg cataggtctg agcaggttta aacggatttt aaaacaattt taagcgccct
     6301 catctcggct ggctcggttg cgttgctcta agctgtgaat gaggtcgtgt acacgttgtt
     6361 tatcggcaca ggttaggttg gtgttattat ccaccgtgca gccggcaatc accgtggcgt
     6421 tatcgccgtt ttgaatgatg acgttgctgt tattaaacac ctgtataggc tcttggcttg
     6481 gctggctgtt gccggcagtg ctattgtctc tgaataattt gagaatggct tgtttgtcca
     6541 tattgtcccc tgttatgcca ccagtttgag taatcccacc attttttctt tttcgctttt
     6601 ttcttgcgtg agaagctcgt agaaagccca caccatttgg gcttttccgg caggcttcat
     6661 ttcccgttgg gtaatttcca gcacctcttc caacgtttct attgcctctt gtaacttagc
     6721 tttgataaca actacttccc cttcttctgt ctgcggtttt gtggcataca caggctgtgg
     6781 ctctcttgtg acattaaggc tttgcgagtg tcgctcacct gttaataggt agcaaggctc
     6841 aacgttaagt atttgtgaca aagccataag ttttgatata ggaggttctc ttgtatcagc
     6901 ctcccacgct tgtaatgtag atattgcaat acctcctagg gcatcacaaa cctcagatct
     6961 acttaatttt gcattttttc tgctctgttc tattcggtta ccgaaaccaa taacctttt
//
LOCUS       DANLYI010000130         6991 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.131, whole genome shotgun sequence.
ACCESSION   DANLYI010000130 DANLYI010000000
VERSION     DANLYI010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 6991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6991
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.131"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            <1..1364
                     /locus_tag="PXD81_001805"
     CDS             <1..1364
                     /locus_tag="PXD81_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005617456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Na+/H+ antiporter family protein"
                     /protein_id="HDL5537812.1"
                     /translation="VFISIIVLLILSLLRINVVIALIISALTCGLIGNLGVEGAGFTE
                     ALEKTIKSFTGGLGGGAETAMNYAVLGAFAVALSKSGVTDLLAYKVIKSFGHSPSGRS
                     VTWFKYIVLFVLVAFAMSSQNLIPIHIAFIPILIPPLLSVMNKLQIDRRAVACALTFG
                     LTATYMLIPAGFGQIFIESILVKNINQAGEPFQLVTTTAEMTKAMLIPVSGMILGLLF
                     AFFVSYRKPRVYIENMPEPTADDIEARVANVKPFHIGISIVAIIATCSVQLATNSTII
                     GGLVGLVIFALGGVFRLKESNDVFQAGLRLMAMIGFVMIAASGFAGVVNSTGGVPALV
                     ESLRDVISSKEMAALLMLVVGLFITMGIGSSFSTVPIITSIYVPLCISFGFSPLATMA
                     IVGVAAALGDAGSPASDSTLGPTSGLNADGKHDHIWDSVVPTFIHFNIPLLVFGWIAA
                     MTL"
     gene            1462..2265
                     /gene="xthA"
                     /locus_tag="PXD81_001806"
     CDS             1462..2265
                     /gene="xthA"
                     /locus_tag="PXD81_001806"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HDL5537813.1"
                     /translation="MKFISFNINGLRARPHQLEAIIEKHQPDVLGLQEIKVADEDFPY
                     DLVNHLGYHVFHHGQKGHYGVALLTKKEPIAVRKGFPTDVLDAQKRMIMADIETDFGV
                     LTVLNGYFPQGENRSHETKFPAKQKFYADLQNYLESELKPENPIIIMGDMNISPTDLD
                     IGIGEPNRKRWLRDGKCSFLPEEREWLDRLYSYGLVDTFRAGNPDVNDKFSWFDYRSK
                     GFDDNRGLRIDLILASKSLAERCVETGIDLDIRAMEKPSDHSPVWATFK"
     gene            2419..3003
                     /gene="lptC"
                     /locus_tag="PXD81_001807"
     CDS             2419..3003
                     /gene="lptC"
                     /locus_tag="PXD81_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HDL5537814.1"
                     /translation="MNKRLTVILLIIVAVLGGWYYSLQEKENTGLDQLIKKEGQPEYV
                     GNKMSTTVYDLKGNPQYFAQATEIKRYESTERTEFFKPFIELFAKESTQKQWKVSADY
                     AEITKEKMLNLKGNIKLVALDPASRLQQITTDTLTVDLNTQDIFTESTVKSIGSGFTT
                     TGVGLKGNLKQQVATLQKDVKSYIEPTIIQETKE"
     gene            3027..3533
                     /gene="lptA"
                     /locus_tag="PXD81_001808"
     CDS             3027..3533
                     /gene="lptA"
                     /locus_tag="PXD81_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide transport periplasmic protein
                     LptA"
                     /protein_id="HDL5537815.1"
                     /translation="MKLMFKSILVSVLLGSSFSAYALKGDTDQPINIDSGSQSLDMTN
                     NIVTFSDNVVITQGSIKVTAANVKITRQEGKKETIDATGSPVTFQQTLDNGKPVNGKG
                     NSVHYDLNSEFLTLIGNAELKQQGSFIKANKITYDVKKQQLKATSGGKTRVKTVLIPN
                     ELQDNKKK"
     gene            3545..4270
                     /gene="lptB"
                     /locus_tag="PXD81_001809"
     CDS             3545..4270
                     /gene="lptB"
                     /locus_tag="PXD81_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005727816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HDL5537816.1"
                     /translation="MSVLYAEHLAKSYKQRQVVKDVSLNVKSGEIVGLLGPNGAGKTT
                     TFYMVVGLVRHDHGTIRIDDQDISTLPMHDRAKQGIGYLPQEASIFRRLSVYDNLMSV
                     LQVRKNINDKQRKERAEELITEFHIEHIRNSLGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKKIIVNLKERGLGVLITDHNVRETLDVCERAYIVGSGEMIAS
                     GTPQQVLENPDVKRVYLGDQFKL"
     gene            4312..4824
                     /locus_tag="PXD81_001810"
     CDS             4312..4824
                     /locus_tag="PXD81_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDL5537817.1"
                     /translation="MTKLTEFLSPENVRQGVLISSKKRALELVGKIVAESINQTEQQS
                     EALCPIECFGNLFKREKLGTTGLNQGIALPHAKLPACGDIELTKPIAVFLQLEEAIDY
                     EAQDNKEVDLIYAMMFPENSCEQYKSCLPAIAQKLTDKSLLKQLRAAESAEDIWQILV
                     YADNHLKDEE"
     gene            4837..5700
                     /gene="rapZ"
                     /locus_tag="PXD81_001811"
     CDS             4837..5700
                     /gene="rapZ"
                     /locus_tag="PXD81_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005600433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HDL5537818.1"
                     /translation="MELIIISGRSGSGKSVALRALEDAGYYCVDNIPLPLIPTLTYYL
                     TQENRSAVISLDIRNLPNTPNAIEELLTQLEPLNAKLIFLDCERNTLIRRYSDSRRIH
                     PLSTQDDLSLEGALDLEQKLLEPLVQNANYIIDTSHLSSHELAENLRQILQGSTEKEL
                     KIIFQSFGFKYGLPADSDYVFDVRFLPNPHWNPELRPMTGLEQPVIDFLERQTEVHNF
                     IYQTRNYLEMWLPMLEQNNRSYLTIAIGCTGGKHRSIFIAEQLAKYFQAKGKNVQVRH
                     KSLEKHHKKTS"
     gene            5783..6967
                     /locus_tag="PXD81_001812"
     CDS             5783..6967
                     /locus_tag="PXD81_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005600432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HDL5537819.1"
                     /translation="MKSADIVIVGGGMVGLALAGLLKDVDCQIKIIEKNTPKFDADTI
                     YHRVSAINASSQKILEQIGAFQHIPAQRLSPYTEMKVWEKDSFANIHFDNNDNEIKQL
                     GLSQLGFILENAVIQHALWQQVSQQANVEYIIAAPQTLGVSENGAFLTLDNGEMLSAK
                     LVVGADGANSWVRNQMKMPLTSKDYQHTALVCNVKTTEKHGKTARQIFAEDSIFAFLP
                     MADEHLCSIVWSLPPNKANELKNASEAEFNKALTIAFDNQLGLCEVQSLREIYPLAAR
                     FCRDFAQPRIALIGDAAHTIHPLAGLGVNLGFADARTLANEIQKQLVNNQDIGEYRHL
                     RQFERERKAQAMKLLATMEGLKQLFDGNHPLKKLFRGIGLSLTNQLPFVKKQLIQQAI
                     YL"
BASE COUNT         2129 a         1433 c         1439 g         1990 t
ORIGIN      
        1 aagtctttat ttcgattatc gtgctgttaa tcctaagctt gcttagaatt aatgtagtaa
       61 tcgcacttat tatttctgcc cttacttgcg gtctaatcgg taatcttggt gtagaagggg
      121 ctggatttac cgaagcattg gaaaaaacaa taaaaagttt tactggtggt ttaggtgggg
      181 gtgcggaaac tgctatgaac tatgccgttc ttggtgcgtt tgccgtagct ctatcaaaat
      241 caggcgtgac cgatttatta gcctataaag tgatcaaatc ctttggtcac agcccgtcag
      301 gtcgctccgt tacttggttt aaatacattg tattatttgt gttagtggca tttgcaatgt
      361 catctcaaaa cctaattcca attcatattg cctttattcc gattttaatt ccaccgctat
      421 taagcgtaat gaataaattg caaattgacc gccgtgcggt agcttgtgct ttaacctttg
      481 gtttaaccgc cacttatatg ttaattccgg caggtttcgg tcagatcttt attgaaagca
      541 tcttagttaa aaatatcaac caagcaggtg aaccgttcca attagtgact accacagcgg
      601 aaatgaccaa agcgatgtta attcctgttt ccggtatgat tttaggctta ctgtttgcat
      661 tctttgtgtc ataccgtaaa ccgcgtgtgt atattgaaaa tatgcctgaa ccaaccgcag
      721 atgatattga agcccgtgtt gcaaacgtta agcccttcca tatcggcatt agtattgtgg
      781 cgattatcgc gacctgttcg gtgcaattag caacaaattc gactattatc ggcggcttag
      841 tcggtctggt tattttcgcc ttaggcggtg tatttagact gaaagagagt aacgatgtct
      901 tccaagctgg cttacgttta atggcgatga ttggctttgt gatgattgcc gcctccggtt
      961 ttgccggcgt ggtcaattca acaggcggtg tgccggcatt ggtagaaagc ctgcgtgatg
     1021 tgatttcatc aaaagaaatg gcagcattgc taatgttagt cgttggctta tttattacga
     1081 tgggtatcgg ctcttcattc tcgactgtgc cgatcattac ctcaatttat gtgccgcttt
     1141 gtatctcatt tggcttctcg ccactggcga caatggcgat tgtcggtgtt gctgctgcac
     1201 tcggtgatgc aggttctccg gcatcagatt ccacacttgg tccaacctcc ggcttgaatg
     1261 ctgacggtaa acacgaccac atttgggatt ctgttgtacc aacttttatc cactttaaca
     1321 ttccattatt agtgttcggc tggattgcag caatgacact ttaagctata atatgcaaaa
     1381 tttttgctaa aaatgaccgc ttgtagccat tacaagcggt ctattttctt ctttattttg
     1441 caaaccacat taggatcacc aatgaaattt atctctttta atatcaacgg attacgagcc
     1501 agaccacatc agctcgaagc gattatcgaa aaacaccagc ccgatgtact cggcttacaa
     1561 gaaatcaaag ttgccgatga agattttcct tacgatttag tcaatcactt aggctaccac
     1621 gttttccacc acgggcagaa aggtcattac ggcgtggcat tactgactaa aaaagagcct
     1681 atagcggtgc gtaaaggctt cccgacagat gtacttgatg ctcaaaaacg aatgattatg
     1741 gcggatatcg agaccgattt cggcgtgcta accgtattaa acggctattt cccacagggg
     1801 gaaaatcgca gccacgaaac caaattcccg gcaaaacaaa aattctatgc tgatttgcag
     1861 aactacttgg aaagcgaatt aaagccggaa aatccgatta ttattatggg cgatatgaac
     1921 atcagcccga ctgatttgga tatcggcatc ggcgagccaa accgcaaacg ctggctgcgt
     1981 gacggtaaat gttcattctt acccgaagag cgtgaatggc tagatcgctt atacagctac
     2041 ggcttggttg atactttccg tgcaggcaac ccagacgtga acgataagtt ctcttggttt
     2101 gactaccgtt caaaaggttt tgatgataat cgtggcttgc gtattgattt aattcttgct
     2161 tcaaaatcac ttgctgagcg ttgcgtggaa acaggcattg atttagacat tcgagcaatg
     2221 gaaaaaccgt ctgaccactc ccctgtttgg gcaaccttta aataaagttg caaaaaaatc
     2281 tgagttttca accgcttgtc agtttttagc cctacaagcg gttatttttt ctcacttttt
     2341 tgcacttttt acgctgtact atatccctat tcaaatctgt gctacaatca aacactattt
     2401 ttagattatt tttggattat gaacaaacgt ttaaccgtta ttttactgat tattgttgcc
     2461 gtacttggtg gctggtatta cagcttacaa gagaaagaga atacggggct tgatcagctt
     2521 attaaaaagg aaggacagcc tgaatatgtg gggaataaaa tgtccaccac ggtctatgat
     2581 ctaaaaggaa acccacaata tttcgctcaa gctactgaaa ttaaacgcta tgagtctact
     2641 gagcgtactg agttttttaa accttttatt gagctttttg ccaaagagtc gacgcaaaaa
     2701 cagtggaaag tcagtgccga ttatgcagaa attaccaaag aaaaaatgtt aaatttaaaa
     2761 ggtaatatca aacttgttgc attagatcct gcatcacgtt tgcaacaaat cactaccgat
     2821 acgttaactg ttgatttaaa tacacaggat atttttaccg aaagtacggt caaatcgata
     2881 ggttccggtt ttactacaac gggggttgga ttaaaaggta acttaaaaca acaagttgcg
     2941 actttgcaaa aagatgtaaa atcttacatt gaaccgacca ttattcaaga aactaaagaa
     3001 taatttgctt ggaacaagga ataacaatga aattaatgtt taaatctatt ttagtgagcg
     3061 tgttactggg tagcagcttt tccgcttatg ctctaaaagg cgataccgat cagccgatta
     3121 atattgattc aggtagccaa tcgttagata tgactaacaa tatcgtgacc tttagcgata
     3181 atgtcgttat cactcaaggg tcgattaaag tgaccgcagc aaacgtcaaa attacccgtc
     3241 aagaaggtaa aaaagaaact attgacgcta caggctcacc tgtaacattc caacaaacct
     3301 tagataacgg taaaccggta aacggtaaag gcaacagcgt tcattatgat ttaaactctg
     3361 aatttttaac gctgattggc aatgcggaac tcaaacaaca aggtagcttt attaaagcca
     3421 ataaaattac ctatgatgtg aaaaaacaac aacttaaagc taccagtggc ggtaaaaccc
     3481 gagtgaaaac cgtgctaatt ccaaatgaac ttcaagataa caagaaaaaa taaggttaaa
     3541 tgatatgtct gtgctttatg ccgaacattt agcaaaaagc tataaacaac gccaagtggt
     3601 aaaagacgtc agcctgaatg tcaaatcagg tgaaatcgtg ggcttgctcg gtccgaacgg
     3661 cgcaggtaaa accaccactt tctatatggt tgtgggtttg gttcgccacg accacggcac
     3721 aattcgtatt gacgaccaag atatcagcac actaccaatg cacgacagag caaaacaagg
     3781 gattggctat ctaccgcaag aagcatctat tttccgccgt ttaagtgtgt acgataactt
     3841 aatgtcggta ttacaagtgc gtaaaaatat caatgataaa cagcgtaaag agcgtgccga
     3901 agagctgatt acagaattcc atattgagca tattcgcaat agtttaggtc aatcactttc
     3961 aggtggtgaa cgccgccgtg tggaaattgc ccgagcatta gcagcgaacc cgaaatttat
     4021 tctgttagat gaaccttttg ctggggttga cccaatctcg gtaattgata ttaaaaaaat
     4081 tatcgtcaat ttaaaagagc gtggtttagg ggtattgatt accgaccata acgtacgtga
     4141 aactctagat gtatgtgaaa gagcctacat tgtcggtagc ggcgaaatga ttgccagcgg
     4201 cacaccacag caagtgcttg aaaaccctga tgtaaaacgt gtttatttag gcgatcaatt
     4261 caaactctaa aaaatcccct ctttggggat ttcatcttta gatgaaaaat tatgacaaaa
     4321 ctaaccgaat ttctaagccc tgaaaatgtt cgccaaggtg tactgatttc aagtaaaaaa
     4381 cgagcgttag aactcgtggg taaaatcgtg gctgaatcaa tcaaccaaac cgaacagcaa
     4441 tctgaagcac tctgccctat tgaatgcttt ggcaatttat ttaaacgtga aaagttaggc
     4501 acaaccggct taaatcaagg cattgcactt cctcatgcaa aattgcccgc ttgcggtgat
     4561 attgagctga ctaaaccgat tgcggtcttt ttacaactgg aagaagcgat tgactacgaa
     4621 gcccaagaca acaaagaagt ggatttaatt tatgcaatga tgtttccgga aaatagctgc
     4681 gaacagtata aaagttgcct acctgcaatt gcacagaaac ttaccgataa atcgttatta
     4741 aaacagctcc gagcagctga atccgcagaa gatatttggc aaattttggt gtatgctgat
     4801 aatcacctaa aagatgaaga ataatactga ggaaatatgg aattaatcat tataagcgga
     4861 cgctcaggct ctggtaaatc ggtagcatta agagcattag aagatgcagg ttattactgc
     4921 gtggataata tccctttacc tttaattcca accttaacct actaccttac tcaagaaaat
     4981 cgttcggcag tcatcagctt agatattcgt aatttaccga atacgcctaa tgcgattgaa
     5041 gaattgctta cccagcttga gcctttaaat gctaagctga tttttttaga ttgcgaacgc
     5101 aataccttaa tccgccgtta tagcgattcc cgccgaatcc acccgctttc cacccaagac
     5161 gatctctcgt tagaaggtgc attggatttg gaacaaaaat tgcttgagcc tttggtgcaa
     5221 aatgcaaatt atattattga caccagtcat ctttcttcgc acgaattagc ggaaaatctt
     5281 cgccaaatac tgcaaggctc aaccgaaaaa gagcttaaaa ttattttcca atcattcggt
     5341 tttaaatacg gcttacctgc cgattccgat tacgtttttg atgttcgctt tttaccgaac
     5401 ccacattgga atccggagct tcgcccaatg acaggcttag agcaaccggt gattgatttc
     5461 ttagaacgcc aaaccgaagt gcataatttt atttaccaaa cccgcaatta tttagaaatg
     5521 tggctgccga tgttggaaca aaataaccgt agctacctaa ctattgcgat tggttgcact
     5581 ggtggaaaac atcgttccat atttattgcc gagcaactgg caaaatattt ccaagcaaaa
     5641 ggcaaaaatg tgcaagttcg gcataaaagt ttggaaaaac accataaaaa aacatcttaa
     5701 ataagtaggg gctggtcctg tgccagccct catttcaact attttagggc gggcacaggg
     5761 caccgcccct acatttgatt ttatgaaatc cgcagatatt gttattgttg gcggcggtat
     5821 ggttgggctg gctcttgccg gactgctcaa agatgtcgat tgccaaatta aaattattga
     5881 gaaaaacacg cccaaatttg atgccgatac gatttaccac cgtgtaagtg cgattaatgc
     5941 ttcaagccaa aaaatactgg aacagattgg tgcttttcaa cacattccgg ctcaacgcct
     6001 ttctccttac accgaaatga aagtgtggga aaaagacagc tttgctaata tccattttga
     6061 taataatgat aatgaaatca agcaattagg actttcacaa cttggcttta ttttggaaaa
     6121 tgcggtgatt caacacgcat tgtggcaaca ggtttctcaa caagctaatg tagaatatat
     6181 tatcgccgcc ccccaaactc tgggcgtgag cgaaaacggg gcatttttaa ccttagataa
     6241 cggcgaaatg ctgtctgcta aattggttgt cggggcagac ggtgcaaatt cgtgggtgcg
     6301 taaccagatg aaaatgccgc ttaccagtaa agattatcaa cataccgcct tagtctgtaa
     6361 tgtcaaaaca accgaaaagc acggcaaaac cgctcgtcaa atctttgctg aagatagcat
     6421 ttttgctttt ctgccaatgg cagatgagca tctctgctcg attgtttggt cgctgccacc
     6481 aaacaaagcc aatgaattaa aaaatgcaag cgaagccgaa tttaataaag ccttaacgat
     6541 tgccttcgat aatcagcttg gcttatgtga agtgcaaagt ttgcgtgaaa tttacccgct
     6601 tgcagcacgc ttctgccgtg actttgccca acctcgtatt gcgttgattg gtgatgccgc
     6661 tcataccatt cacccattgg cgggacttgg tgtcaattta ggctttgccg atgccagaac
     6721 gctggcaaac gaaatccaaa aacagttggt aaacaaccaa gatattggtg aataccgcca
     6781 tttacgccaa tttgaacgtg agcgtaaagc ccaagcgatg aaattattag ccacaatgga
     6841 aggattaaaa cagctatttg acggcaatca cccgcttaaa aaattattca gaggaattgg
     6901 tttaagccta accaaccagc tgccttttgt gaaaaagcag cttattcagc aagctattta
     6961 cttataaaat gtaaattttt tcgaaataac g
//
LOCUS       DANLYI010000131         6978 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.132, whole genome shotgun sequence.
ACCESSION   DANLYI010000131 DANLYI010000000
VERSION     DANLYI010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 6978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6978
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.132"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            14..1276
                     /locus_tag="PXD81_001813"
     CDS             14..1276
                     /locus_tag="PXD81_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HDL5537820.1"
                     /translation="MGTLNSFLGIIVLLFIAFLFSSNKRAINVRTVLGALALQVAIGA
                     LVLYVPAGRDALNAMATGVSKVISYGNEGISFLFGGLVSDKMFEVFGGGGFVFAFRVL
                     PVIIFFSALISLLYYIGVMQWVIRILGGLLQKALGTSKAESMSAAANIFVGQTEAPLV
                     VKPYISKMTESELFAIMAGGLASIAGSVMAGYAGMGVPLTYLIAASFMAAPAGLLFAK
                     IMVPQTEKFRDELEAVDLEKPANILDAAASGASSGMQLALNVGAMLIAFVALIALVNG
                     ILGGIGGWFGYDTLNLGQIFGWVFKPLAWVIGVPWEEAEIAGQMIGLKLAVNEFVGYL
                     EFAKYLAPESTVHLTDKTVAIITFALCGFANFSSIAILIGGIGAMAPSRRGDIARLGI
                     KAVIAGSLANLMSATIAGLFIGLGGAVL"
     gene            1421..2197
                     /gene="deoC"
                     /locus_tag="PXD81_001814"
     CDS             1421..2197
                     /gene="deoC"
                     /locus_tag="PXD81_001814"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HDL5537821.1"
                     /translation="MNLKKSAEIALSLMDLTTLNDNDTDEKVIALCQQANTKFGTPAA
                     ICIYPRFIPIARKALKQQGIEQVKIATVTNFPHGNDDIEIAVAETKAAVAYGADEVDV
                     VFPYKALIAGNEQIGFDLVKQCKAVCAANNVLLKVIIETGELKSAELIRKASEISIQA
                     GADFIKTSTGKVAVNATLESARIMLETIRDLGVADRVGFKAAGGVKTSEEAAQYLALA
                     ESILGNDWVSASHFRFGASSLLANLLATLNDEQQQAVQGY"
     gene            2218..2937
                     /gene="deoD"
                     /locus_tag="PXD81_001815"
     CDS             2218..2937
                     /gene="deoD"
                     /locus_tag="PXD81_001815"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HDL5537822.1"
                     /translation="MATPHINAPEGAFADVVIMPGDPLRAKYIAETFLEDAKEVTNVR
                     NMLGYTGTYKGRRISVMGHGMGIPSCSIYAKELITEYGVKKIIRVGSCGAVRNDVKVR
                     DIVIGVGACTDSKVNRIRFRDNDFAAIADFDMTLAAHQAAKAKGIEARFGNLFSADLF
                     YSPDVEMFDVMEKYGILGVEMEAAGIYAVAAEYGAKALAICTVSDHIRTGEQTTSEER
                     QLTFNGMIEIALESVLIGDKQ"
     gene            complement(3013..3864)
                     /locus_tag="PXD81_001816"
     CDS             complement(3013..3864)
                     /locus_tag="PXD81_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDL5537823.1"
                     /translation="MQTHRAIIESIFALAPKNELWATPISGLTVHAADRSIRFNGYIQ
                     DPSLCVVLLGERQICTNGHCQVFGNQEMMFCPVNLPIVTQTLSVAENQPYLAISLRLD
                     FDLIAKIITQLPPQKISENINQRTKWELENEIKDCLLRLLNLLNSPNHIHFLAPLILN
                     ELYFYLLQSEQGGYIRSLLSQDGNIAKIANATKILERKFADTLNMSALAEEVGMSVAG
                     FYAHFKSVTSMTPLQYQKSLRLNAAREKLQAGVSVSETAYQVGYESPSQFSREYKRQF
                     GESPKGR"
     gene            3979..4410
                     /locus_tag="PXD81_001817"
     CDS             3979..4410
                     /locus_tag="PXD81_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HDL5537824.1"
                     /translation="MKIFYHTSATATGGRDGRTQVDDGSIGFDLVGFQNESGKVGTNP
                     EQLFAMGYAACFDSAMNHVAPTLGIKPTKSSTTVAVGIGQKADGAFGLDLDITITVEG
                     ISAEEAKTLISKAHEVCPYSNATRGNVDVRLHVKVVSEFDL"
     gene            4427..5107
                     /locus_tag="PXD81_001818"
     CDS             4427..5107
                     /locus_tag="PXD81_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HDL5537825.1"
                     /translation="MKMLDFETTVKTRQSIRKFLPLPMTETEIKQILADAQNAPSACN
                     TQPWLVHVASGETLARLKARFKKTFEAGINDADFEFNQTKFTGDYEPRWRNQYSHVFT
                     TSYGIPREDKAGRQVVYDDNIVGYGAPHMAFLFMPSIDGHDVNIASDVGMYSQTFLLS
                     LTARGFGGIPQLALAMFAKDVREELGIPEHYKLLHAISFGHPDWDAMQNKHHLGRVPV
                     EESATLHW"
     gene            5171..6295
                     /locus_tag="PXD81_001819"
     CDS             5171..6295
                     /locus_tag="PXD81_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HDL5537826.1"
                     /translation="MINLFTPYTLNNDVEIKNRLVVAPMTHSASNDDGTISEQERHFL
                     HNRAADMGLFITAATGVMRNGKAFYGQPEALDERYLASLTETANILKNQGTKAILQIF
                     HGGAQAIPALLDGANAVAPSEHANGAKALTADEVKQMIQAFANATDLAIQAGFDGVEI
                     HGANGYLIQQFFSTEFNQRTDEWGGSLEKRLRFPLAVVEAAVSVREKHQRPDFIIGYR
                     FSPEEPGEHGLTMTDTYALIDALCEKPLQYLHISLWDFGKKARRGADTNFTRMQLVHQ
                     HINGRLPLIGVGNLLTGEQIRKAYATGWAEFIALGKAVMLNPNVATLLKDNRDSEIVS
                     ELDPQQADHYGIPDRLWGYCVQGGAWLPPLKGQNGQPMDI"
     gene            6379..>6978
                     /locus_tag="PXD81_001820"
     CDS             6379..>6978
                     /locus_tag="PXD81_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018650747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HDL5537827.1"
                     /translation="MITLHYLNSSCSHRIVWLLEALGLDYELKIYHREESGFAPMEVK
                     ALHPLGKAPVLQDGDLVLAEGNAIIQHLIARYAEGKFEPTRGSEAYSMYHYWLSISAS
                     LFSANLVTMLANRSDLGAFGDYAKAQTPLYFNHIEQTLSDKKWIVGDELTGADFSLCF
                     PLQWGRNFVEMANYPNIARYLEQVENHPAYQKANQKALKL"
BASE COUNT         1961 a         1411 c         1597 g         2009 t
ORIGIN      
        1 aattagaggt tctatgggta ctcttaacag ctttttaggc attattgttt tactattcat
       61 tgcattttta ttttcgagca ataaacgtgc aataaacgtg cgtaccgtat taggcgcatt
      121 agcattgcag gtagctattg gtgcattggt gctttatgtg ccggcagggc gtgatgcctt
      181 gaatgcgatg gcaacaggtg tgtcaaaagt gatttcttac ggtaatgaag ggatttcatt
      241 tttattcggt ggcttagtca gcgacaaaat gtttgaagta tttggcggcg gcggctttgt
      301 gttcgctttc cgtgtacttc cggtgattat tttcttctct gcactcattt ctttgcttta
      361 ctacattgga gtgatgcaat gggtaatccg tattttaggt ggtttattac aaaaagcatt
      421 aggtacatct aaagcggaat ctatgtctgc ggcagctaat attttcgttg gccaaactga
      481 agctccattg gtagtaaaac cttatatcag caaaatgaca gaatctgaac tctttgcgat
      541 tatggcaggg ggcttagctt ctattgcagg ttctgttatg gctggttacg caggaatggg
      601 cgtaccatta acgtacttaa tcgcagcctc ctttatggcg gctccggcag ggttattatt
      661 cgccaaaatt atggtgccac aaaccgaaaa attccgtgat gaactagaag cggtagattt
      721 agaaaaaccg gcaaatattc ttgatgcagc agcttcaggt gcttcttcag gtatgcaact
      781 ggcattaaac gtaggggcaa tgttaattgc attcgtggcg ttaattgcat tagtgaatgg
      841 tattttagga ggtattggcg gctggttcgg ttatgatacc ttaaacttag gtcaaatttt
      901 cggctgggta tttaaaccgc ttgcgtgggt aatcggtgtg ccttgggaag aagcagaaat
      961 tgcggggcag atgatcggct taaaattagc ggtaaatgaa ttcgtgggtt atttagagtt
     1021 tgctaaatac ttagctcctg agtcaaccgt acatttaacg gataaaactg ttgcgattat
     1081 tacctttgca ctttgtggct ttgcgaactt cagctcgatt gcgattttaa tcggtggtat
     1141 cggggcaatg gctccaagcc gtcgtggtga tattgcccga cttgggatta aagccgtcat
     1201 tgctggctca cttgccaact taatgagtgc aaccattgcc ggcttgttta tcggcttggg
     1261 tggagcagtt ctctaatcca gtttgtataa aaccactaga aatagtggtt ttatcttttt
     1321 ttatctgacg taaacggttg cttttggttt gtctatttac aagcggtcag atttttataa
     1381 aattttacca accaccacgc atttaataaa aggagacact atgaatttaa aaaaatccgc
     1441 tgagattgct ctttcgttga tggatctcac cacattaaat gacaatgata ctgatgagaa
     1501 agtcatcgca ttatgccaac aagcgaacac caagtttggc acaccggcag caatttgcat
     1561 ttatccacgt tttatcccga ttgcccgcaa agcattgaaa cagcaaggta tcgagcaagt
     1621 aaaaattgcg acagtcacga atttccctca cggtaatgat gatattgaaa ttgcggtagc
     1681 tgagaccaaa gccgcagttg cttatggggc agatgaggta gatgtggtat tcccttataa
     1741 agcattaatt gcaggtaatg agcaaatagg tttcgacttg gtaaaacaat gcaaagcagt
     1801 ttgtgctgcc aataatgtgt tgcttaaagt gattattgaa accggcgagt taaaatcagc
     1861 agagcttatt cgcaaagcaa gcgaaatttc gattcaagca ggggctgatt ttattaaaac
     1921 ctcaaccggc aaggtggcag tgaatgctac ccttgaatct gcccgcatta tgcttgaaac
     1981 cattcgtgat ttaggtgtgg cagaccgtgt tggctttaaa gctgcaggtg gggtaaaaac
     2041 ctcggaagaa gccgcccaat atcttgcact tgcggaatct attttaggca atgactgggt
     2101 gagtgcatca cattttcgct tcggtgcatc aagtttatta gctaatttat tagcaacatt
     2161 gaatgatgaa cagcagcaag ctgttcaagg ttattaatta ataaagagga acataaaatg
     2221 gctactccac acattaacgc acctgaaggt gctttcgctg acgttgtaat tatgccgggc
     2281 gatccgcttc gtgctaaata tatcgctgaa acctttttag aagatgcaaa agaagtgacc
     2341 aatgttcgca atatgttagg ctacaccggc acttataaag gtcgccgcat ttcagtaatg
     2401 gggcacggta tggggattcc atcttgctcg atttacgcta aagaattaat tactgaatat
     2461 ggcgtgaaga aaattatccg tgtcggctcg tgcggtgcgg tacgtaatga tgtcaaagtg
     2521 cgtgatattg tcatcggtgt tggggcttgt accgattcta aagtaaaccg tattcgcttc
     2581 cgtgataacg attttgcggc gattgctgat tttgatatga cattagctgc tcaccaagca
     2641 gctaaagcga aaggcattga agctcgtttc ggtaatttat tctcagccga tttattctac
     2701 tcacctgatg tcgaaatgtt tgatgtaatg gaaaaatacg gcattttagg cgtagaaatg
     2761 gaagcagcag gcatttatgc ggtagcggca gagtatggag caaaagcatt agcaatttgc
     2821 accgtttcag accatatccg caccggtgag caaactacct ctgaagaacg tcaattaacc
     2881 tttaatggta tgattgaaat tgcgttagag tcagtattaa ttggcgataa gcagtaattt
     2941 ctaacctgac aagcggtgaa attttgcaaa aaatttgcag aatttgaccg cttgttattt
     3001 ttgattcttc ctttatcttc ctttcgggct ttcgccaaac tgccttttat attctcgact
     3061 aaactggctt gggctttcat agccgacttg ataggcggtt tcactcacgc ttactcctgc
     3121 ttgtagtttt tcccttgccg catttagtct taacgatttc tgatattgca gtggtgtcat
     3181 tgaagttacg cttttaaaat gagcgtaaaa ccccgccacg ctcattccta cctcttcagc
     3241 cagtgctgac atatttagtg tatcggcaaa ttttctttct aagatttttg ttgcattggc
     3301 aattttagca atattgccat cttgagagag tagagagcgt atatagccac cttgctcaga
     3361 ttgtaataaa tagaaataga gttcgtttaa tatcaatggt gcaagaaaat gaatatgatt
     3421 tggagaatta agcaaattta gtaagcgtaa caaacagtct ttaatttcat tctccagctc
     3481 ccatttagtg cgttgattaa tgttttcact tattttctgt gggggaagct gcgtgataat
     3541 tttggcaata agatcaaaat ccaagcgtag tgaaatcgca agataaggct gattttctgc
     3601 aactgataag gtttgggtaa caatcggcaa attcacaggg caaaacatca tttcctgatt
     3661 accgaagact tggcaatgtc cattggtaca gatttgccgc tcgcctaata atactacaca
     3721 taaacttggg tcttggatat aaccgttaaa tctaatagaa cgatcggcag cgtgaacggt
     3781 tagccctgaa atgggagtag cccaaagttc atttttcgga gcgagggcaa aaatagattc
     3841 aataatagct cgatgagttt gcataataat ctcactttta gaaaattagg catattttat
     3901 agaaaatcag gcaagacgac aaatagcaaa aagcggataa tatccccaat caaataaagc
     3961 atttaatagg agcaaaatat gaaaattttt tatcacacat cagcaactgc aaccggtggt
     4021 cgtgacggca gaacccaagt ggatgacggt tctatcggtt ttgatttagt cggtttccaa
     4081 aatgaaagtg gcaaagtagg cactaatccg gaacaattat tcgcaatggg ctatgctgcc
     4141 tgctttgaca gtgcaatgaa tcacgttgcc ccaaccttag gcattaagcc aaccaaatcc
     4201 agtaccactg ttgcagttgg gattggtcaa aaagctgatg gtgctttcgg cttagattta
     4261 gatattacca ttactgtgga aggcattagt gcggaagaag cgaaaacttt aattagcaag
     4321 gcacacgaag tttgcccata ttcaaatgca acccgtggta atgtagatgt gcgtttacac
     4381 gttaaagtcg tgagtgaatt tgacttataa aaaataaata ggggaaatga aaatgttaga
     4441 ttttgaaacc acggtaaaaa cacgacaatc aatccgcaag tttttaccgc ttccgatgac
     4501 ggaaactgaa atcaaacaaa ttcttgccga tgcacaaaat gccccttcgg cttgtaatac
     4561 acagccgtgg ttagtacacg ttgcctctgg tgaaacgcta gcacgcttaa aagctcgttt
     4621 taaaaagact tttgaagcgg gcattaatga tgcggatttt gagttcaatc aaacgaaatt
     4681 taccggtgac tacgaaccac gttggcgtaa ccaatacagc cacgttttta ccaccagcta
     4741 tggcattccc cgtgaagata aagccggtcg ccaagtggtg tatgatgaca atattgtcgg
     4801 ttatggtgcg ccacatatgg cattcctatt tatgccaagc attgacggac acgatgtcaa
     4861 tattgccagc gatgttggta tgtattcgca aactttcttg ctctcactca ccgctcgtgg
     4921 ttttggcggc attccgcagc ttgcccttgc gatgtttgct aaagatgtgc gtgaagagtt
     4981 aggcattcct gaacattaca agctcctcca cgccatttct ttcggacacc cagattggga
     5041 cgctatgcag aacaaacacc atttagggcg tgtgccggtg gaagagagtg cgaccttaca
     5101 ttggtaataa acaagcggtt gatttttcgc aaaaatttgc gaaatcaacc gtatttttta
     5161 ggagaatacg atgataaatt tatttacccc ttatactctg aataacgatg tagaaattaa
     5221 aaaccgcctt gtagttgccc caatgaccca ttccgcctcc aacgatgacg gcacaattag
     5281 cgagcaagaa cgccatttct tgcataatcg tgcagcagat atgggcttat ttatcaccgc
     5341 agcaaccggg gtaatgcgta acggcaaagc gttttacggt cagccggaag ctcttgatga
     5401 acgctatttg gcaagcctga cagaaactgc caatatcttg aaaaatcaag gcaccaaagc
     5461 gattttgcaa attttccacg gtggggcaca agctattcct gccttgcttg acggggcgaa
     5521 tgcagttgcg ccgtccgagc acgccaacgg tgctaaagcc ctaacggcgg acgaagtgaa
     5581 gcaaatgatc caagctttcg ctaatgccac cgatttagct attcaagccg gttttgatgg
     5641 tgtggaaatt cacggggcga acggctattt aattcaacaa tttttctcta ctgaatttaa
     5701 tcaacgcact gatgaatggg gcggtagcct tgaaaaacgc ttgcgtttcc cgttagcggt
     5761 ggttgaggca gcggtcagcg tgcgtgaaaa acaccaacgc cctgatttta ttatcggcta
     5821 ccgtttctca ccggaagagc cgggcgaaca cggcttaacc atgaccgata cctacgccct
     5881 gattgatgct ttgtgcgaaa agccgttgca atacttgcac atttcactct gggatttcgg
     5941 caaaaaagcc cgccgtggtg cagataccaa cttcacccgt atgcaactgg tacaccaaca
     6001 catcaacggg cgtttaccgc tgatcggcgt gggtaactta ttaaccggtg aacagatccg
     6061 taaagcttat gcaacaggct gggcagaatt tatcgcctta ggtaaagcgg taatgctcaa
     6121 cccgaacgtg gcgaccttgt tgaaagataa tcgagacagt gagattgtca gcgaactcga
     6181 cccgcaacaa gccgaccact acggcattcc cgatagacta tggggctact gcgtgcaagg
     6241 tggtgcttgg ttaccgccgc ttaaagggca aaacgggcaa ccaatggata tttaatcatt
     6301 acagacaagc ggtcattttt ttgtaaaaat ttgcaattaa gtgaccgcat tttttcaatt
     6361 atctgaagga gaaaaattat gattacgtta cattacctga acagttcctg ctctcaccgc
     6421 atcgtttggt tgttggaagc cttaggcttg gattacgagc taaaaatcta ccatcgtgaa
     6481 gagagcggtt ttgcacctat ggaggtaaaa gccttgcacc cacttggcaa agcccctgtg
     6541 ttgcaagatg gcgatttggt gcttgccgaa ggcaatgcga ttatccagca tctgattgcc
     6601 cgttacgccg aagggaaatt tgagccgact cgtggtagcg aagcctattc aatgtatcac
     6661 tactggttgt cgatttctgc ttcattattc tcggcaaacc ttgtgacaat gttggctaat
     6721 cgctctgatc tcggagcttt tggcgactac gcaaaagcgc agactccgct gtattttaac
     6781 catattgagc aaacactttc agataaaaaa tggattgtgg gtgatgaact aaccggtgcg
     6841 gatttttctc tctgtttccc actacaatgg gggcgtaatt ttgttgaaat ggcaaattac
     6901 ccgaatattg cacgctattt agaacaggtg gaaaatcacc ctgcttacca aaaagcgaat
     6961 caaaaagccc tgaagctt
//
LOCUS       DANLYI010000132         6879 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.133, whole genome shotgun sequence.
ACCESSION   DANLYI010000132 DANLYI010000000
VERSION     DANLYI010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 6879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6879
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.133"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            complement(247..2595)
                     /locus_tag="PXD81_001821"
     CDS             complement(247..2595)
                     /locus_tag="PXD81_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005612269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HDL5537828.1"
                     /translation="MLISAQFSPLVWAENNDVAVLDEVSVVGSTPSISQGSEVTLLKV
                     SDKIIAGKEFKKRSATLGNALASELGVHSNPFGGGASKPIIRGQEGARIRILQNGSDV
                     IDMSNLSPDHAVVADSLLADQVEILRGSSTLLYASSSPVGIVNVVDKRIPTAIPEKGY
                     EVELNSRFDTAAKEKVGALGATFGIGNHIAVRVEGLSRHSDNYRVPGINLGERLNYVP
                     DTYNKSKVGTLGLSFVGERGYIGASYSKRRDNYGLPGHNHKFDFCTGHIYGNKRDKYA
                     YTYLYPHLIGEENIGSNPHFHCGTNHAEDGTHSHDNPFGHAHDHTHKGPWVDLESKRI
                     DVKAELRQPFKGVDKIKASYADADYYHDEKDAGVLATRYHKQLKKDQDYGKPVNIFKN
                     RGKNTRLEVYHAPLGGLTGVWGVQYQTQKSSMNAPKDREVKFPLVENTNKQFSLFGVE
                     QYMWDSVAVELAGRMEKQKIEIEYDRNEIKRLQEHYRISGGKQVEPDLSPYDETAYAY
                     SGTLNWFFHPDYQLSFTASHNERLPTPMELYYHGQHIATNSFEYGNKDLKKEQSNNVE
                     LGLGYQAERIGYKVSVYYNHFKNYIYNENLFRENQLFMRRYNQAKARFYGVEAEASYR
                     FNDQYQATIFGDMVRGWLTNLPPLKINSDYSVFKDYLPENVEKGKDYLLYRADQNTPR
                     TPPMRLGFRFNAEFTPNWSGDLELIRTFTQRRTSQLEYITEGNTMLNVGLSYSNKWKE
                     LDYKISLNGTNLLNQPVYIHTSYHQFVPQMGRNFMLGMEMKF"
     gene            2780..4237
                     /locus_tag="PXD81_001822"
     CDS             2780..4237
                     /locus_tag="PXD81_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5537829.1"
                     /translation="MRSTFKLTLAALAVSIVLSGCKSSQGPSIVADSPPPKEITATDG
                     NDKEKTDNKEKKSAQPNKSDPQPDKPANAKDPVKPDAETNKPSKKDKEYTPPATEKID
                     NSSVQPDKMVPNPIENKENDSNKPNPKESDSDNNDKVTEGNKPNAPTETVNKDKEAEK
                     SKKENKEDSSNNTPNINFDGAKPIDTDSKGSPYRVINLNPRKSVVNASAMQKVDFDSF
                     TDESVENSPFVSSDTEKEYNNTLVFGLGAGDKDSYFKNLNQKGDKHLGIHSGVYEDAN
                     LEGNTKKINYLYVNQPYSSYGALFTDQKDSHLFNVRLTAGRDDVKENGDSYAEYGVFT
                     QSGKEAKWNDNLIGDATYKGQAIARVIRQVNGEQVAGIPQFDGDVTLNLHLSKEWNES
                     KLNGKIDSQSVGVITLKESEIANPTALRSNIGFGEEVIVENKENLVGDYAVDFTGPQL
                     EEAVGVVELENEAETFSENEVIKYNAVFGVSKAAK"
     gene            4301..5713
                     /locus_tag="PXD81_001823"
     CDS             4301..5713
                     /locus_tag="PXD81_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005604600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF560 domain-containing protein"
                     /protein_id="HDL5537830.1"
                     /translation="MKKIALLALFPMMLFARDPNTTQAYIEHTAQPTTTPQRVKIEKS
                     VPIFNETELRANQLLTEKLLIQAIYSRRLANMEKLTAIYRDFTHIDPVLLRYAQGKIA
                     LLKGDYAQAIQHFQTILAKQPKLNTVRIELAIALLYDQQLNQAQAQFEKAKSAEHLPP
                     KVHALIEQYQQHLAKKQEWKFSFSANYVRDGNINRTSKQPNIEQTGYVKQPNLLPQTA
                     HGVHYAFGLQRDFNLFDSHYVSFDNQTQGELYWDNHDYDEITNRTLFGYAYKKSDQTT
                     RLLPFYEKRWKHHRSERWTNGIRLEHAQWFSPHWQLATALEYGKKRYFDLPIYNGSYK
                     LASATLLWLRNPQQFFFVGTDFNQDKTQLKQYSSDTKGIRFGWQREWQALELSSRLTL
                     GYTDRRYKDTAILGGKLNLGRIRHDRVQSANLTLWKRDWHWLGLMPKLQFSYAKHRSN
                     LPSLYAYTDKNINLIIESRF"
     gene            complement(5963..6286)
                     /locus_tag="PXD81_001824"
     CDS             complement(5963..6286)
                     /locus_tag="PXD81_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="filamentous hemagglutinin"
                     /protein_id="HDL5537831.1"
                     /translation="MQKAYQIYTHSEREKVTEAKLELGKVETRLRNEGKTKEEIAQHP
                     EYLQLKRDLNDKQQTFDNAYGTGSKTKRAVDAVTAALQGLAAKDSGQAAVGLASPYLN
                     EQIKK"
     gene            complement(6255..6782)
                     /locus_tag="PXD81_001825"
     CDS             complement(6255..6782)
                     /locus_tag="PXD81_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HDL5537832.1"
                     /translation="MRDKNKERQQTLERYQELYDQDPELRVIAANSEGDLDLLQETFF
                     VKGELTITDSTPKEKWNWLHMCNLNSYSPSPLSVVEFYIKHGVDVNAQDCYGMTPLHY
                     AMRAKNVDAAIALLNAGADPNIENKDQVIPLSMIGAVPKEIELIEHDISEVKETQELV
                     KGIGEIAEGLSDLHA"
BASE COUNT         2077 a         1517 c         1300 g         1985 t
ORIGIN      
        1 actttttctc ttgacagatg ctaagttatt tataaataac catattttta ataaagggat
       61 aaagttaatt gtcttattta acaacaaaca ggaaattttg gtaagctccg aaaataacaa
      121 gaaaggaaaa acagcactat tttatccaaa ttaccaatac ctgagtttta aacaagcggt
      181 tatttttagc cgtttttttg caaaatttga agaaaaaata accgcttgtc ttgttatgtc
      241 gagaacttaa aatttcatct ccattcccaa cataaaattt cgtcccattt gtggtacaaa
      301 ttggtgatac gaagtgtgga tatacaccgg ctgattcagt aagtttgtgc cgtttagact
      361 gattttgtaa tccagctctt tccacttatt gctatacgac aacccgacat tcaacatcgt
      421 attaccttcg gtaatatatt ccaactggct cgtacggcgt tgggtaaagg tgcggatcag
      481 ctctaaatcc cccgaccaat ttggggtaaa ttccgcatta aagcgaaaac ctaaacgcat
      541 tggcggtgta cgaggggtgt tttggtcagc ccgatataga agataatctt tccctttttc
      601 cacattttcc ggcagataat ctttgaacac actataatcg ctatttatct tcaatggtgg
      661 taaatttgtt agccagcctc gtaccatatc gccgaaaatc gtggcttggt attggtcgtt
      721 aaagcggtag cttgcctctg cttctacccc gtaaaaacgg gcttttgcct gattataacg
      781 gcgcataaat agctgatttt cacggaaaag attttcattg tagatgtagt ttttaaagtg
      841 attgtaataa acactcactt tatagccaat acgttcagct tgatagccca agcccaactc
      901 cacattattt gactgctctt ttttcaagtc tttattgccg tattcaaacg aattagtagc
      961 aatatgctgc ccgtgataat acagctccat tggcgttggc aagcgttcat tgtgcgaggc
     1021 agtaaacgaa agctgatagt cagggtggaa gaaccagtta agcgtgccgg aataggcata
     1081 agccgtttca tcatagggtg aaagatctgg ctcaacctgt ttgccgccac tgatacggta
     1141 gtgttcttgt aaccgtttga tttcattgcg atcatactca atctcaatct tctgcttttc
     1201 cattcgtccg gcaagctcaa cagcaacgct atcccacata tactgctcaa ccccaaacag
     1261 gctgaactgt ttattggtat tttcaaccag cgggaatttc acttcgcgat ctttcggcgc
     1321 attcatactg cttttttgcg tttgatactg aacaccccat acaccggtta agccacctag
     1381 cggtgcgtga tagacctcca agcgggtatt tttgccccga tttttgaaaa tattcaccgg
     1441 cttgccgtaa tcctgatctt ttttcagctg tttatggtaa cgggtggcaa gcacgcctgc
     1501 atctttttca tcgtggtaat aatcggcatc agcatagctt gccttaattt tatctacgcc
     1561 cttaaacggc tggcgtagct cggctttcac atcaatccgt tttgattcta aatccaccca
     1621 cgggcctttg tgagtgtgat cgtgggcgtg tccgaacggg ttatcgtggc tgtgtgtgcc
     1681 gtcctcggcg tggttggttc cacagtggaa gtgcggattg ctcccaatat tttcctcacc
     1741 aatcaaatgc ggatagagat aggtataagc gtatttatcc cgcttattac cgtagatatg
     1801 accggtacag aaatcaaatt tatggttatg ccccggcaag ccataattat cccgccgttt
     1861 gctgtatgag gcaccaatat agcctcgttc accgacaaag gataagccca acgtgccgac
     1921 tttggattta ttgtaagtgt ccggcacata gtttaagcgc tcgcctaagt tgatccccgg
     1981 cacacggtag ttatcactat gacgactcag cccttcgact cgcaccgcaa tgtggttacc
     2041 gatgccaaag gttgccccta atgcgccgac tttctctttg gcggcggtat caaaacggct
     2101 attgagttca acctcatatc ccttttcagg aatagcggtc ggaatacgtt tatccaccac
     2161 atttacaatt cctaccggcg aactgctggc ataaagcaag gtgctggaac cacgcaagat
     2221 ttcgacctga tcggcaagca gtgaatcagc aacaacggcg tgatcaggag ataggttcga
     2281 catatcaatc acgtctgaac cgttttgcaa aatgcgaatt ctcgcccctt cctgcccacg
     2341 aataatcggc ttactcgctc cgccaccaaa cgggttacta tgcacgccta attctgacgc
     2401 caacgcatta cccagcgttg ccgaacgctt tttaaattcc ttacctgcaa taattttatc
     2461 actgactttt agcaaggtaa cttcactccc ctgtgatata cttggagtgc tgccaacgac
     2521 cgaaacttca tctaacaccg ctacatcgtt attttccgcc caaacaagcg ggctaaattg
     2581 agccgaaatc agcaatgcca atcctgtttt cttaaacaca atctttctcc ttaaaaaaat
     2641 taaggataat aaggtactgc aaaatatgag tctttacaat aataaccata aaaaaattat
     2701 attttttaaa ttttccctat tgccaaacag gaaatatatc cattaaaatt ttatggattt
     2761 aatctgacgg agacaaaata tgcgttctac attcaaactt actcttgcag ctttagctgt
     2821 ttctattgtt ttaagtggct gtaaatctag ccaaggtcca tctattgttg cagacagccc
     2881 tccacctaag gaaataacag ctactgatgg caacgataaa gagaaaaccg ataacaaaga
     2941 gaaaaagtct gcgcaaccaa ataaatccga tcctcaaccg gataaacctg ctaacgcaaa
     3001 agacccagta aaacctgatg cggagacaaa taaaccaagt aaaaaagata aagaatatac
     3061 accacctgct actgaaaaaa tcgataactc atcagtacag ccggataaaa tggtgcctaa
     3121 tcctatcgag aataaggaaa acgacagtaa taaacctaac cccaaagaaa gcgatagcga
     3181 taataatgac aaagtcactg aaggcaataa accaaatgcc ccaacagaaa cagtaaataa
     3241 agataaagaa gctgagaaaa gtaaaaagga gaataaagag gactcaagca ataatactcc
     3301 taacattaat tttgacgggg caaagcctat agatacagat agtaaaggta gcccgtaccg
     3361 agtaataaac ctaaatccga gaaaaagcgt agtaaatgca agtgctatgc aaaaagtaga
     3421 tttcgactct tttacggatg aatctgttga aaattcacca tttgtcagta gcgatactga
     3481 aaaagaatac aataatacac ttgtttttgg gcttggtgcc ggagataaag attcttattt
     3541 taagaactta aatcaaaaag gggataaaca tttaggtatt catagtggcg tgtatgaaga
     3601 tgcgaattta gaaggtaata ctaaaaaaat taactatctc tatgtaaatc aaccttattc
     3661 gagctatggt gctttattta cggaccaaaa agacagccat ttattcaacg ttcgtctgac
     3721 cgctggtaga gatgatgtaa aagaaaatgg agatagttat gccgaatatg gtgtattcac
     3781 acaatcagga aaagaagcta aatggaatga taacctgatt ggtgatgcca cttacaaagg
     3841 tcaagctatt gctcgcgtta ttcgtcaggt aaatggggaa caagtcgcag gtattcctca
     3901 atttgatggt gatgttacat taaatttaca cctttccaaa gaatggaatg aaagtaaact
     3961 aaacggcaaa attgatagcc aaagtgttgg ggtaattaca cttaaagaaa gtgaaatagc
     4021 taaccctacc gcactccgct caaatattgg ttttggagaa gaagtcatag ttgagaataa
     4081 agagaatctt gtgggtgatt acgctgtcga ttttaccggg cctcagcttg aagaggcggt
     4141 aggcgttgtt gaattagaaa acgaagccga gacattctct gaaaatgaag tcattaaata
     4201 caatgcggta tttggcgtaa gcaaagcggc gaaatagtat tttttgtatc ttacaaaggg
     4261 ctgttaagcc cttttatttt tattatttta aggataaaaa atgaaaaaaa ttgccttact
     4321 ggcacttttc ccaatgatgt tatttgcccg agatcctaat actacacagg cttatattga
     4381 gcatactgca cagccaacta caacccccca aagggtcaag atcgaaaaat cagtgcctat
     4441 ttttaacgaa actgaattac gtgctaatca attattgacc gaaaagctac ttatccaagc
     4501 tatttatagt cgtaggcttg ctaacatgga aaaattaact gcgatttatc gggattttac
     4561 ccacattgac ccggttttac tgcgttatgc acaaggtaaa attgccctac tgaaaggcga
     4621 ttacgcacag gcaattcagc acttccaaac cattcttgcc aaacaaccca aactaaacac
     4681 tgtaagaatt gagctggcga ttgctctatt gtacgatcag caacttaatc aagcccaagc
     4741 acaatttgaa aaagccaaaa gtgccgaaca tctaccgcct aaagttcacg cattaattga
     4801 gcaatatcag caacatctgg caaaaaaaca ggaatggaaa tttagttttt cagccaatta
     4861 tgtgcgagac ggcaacatta accgaacctc gaaacagccg aacattgagc aaaccggcta
     4921 tgtaaaacaa cctaatttgt tgccccaaac cgcacatggt gttcactatg cgtttggatt
     4981 acagagagat ttcaacctgt tcgactcaca ctatgtgagt tttgacaacc aaacccaagg
     5041 tgaactttat tgggataacc acgattatga tgaaattacc aatcgcacct tgtttggata
     5101 cgcttacaaa aaatccgacc aaaccacccg cttgttaccg ttttatgaaa aacgctggaa
     5161 acatcaccgt tcggaacgct ggacaaacgg aattcgactt gaacacgcac aatggttttc
     5221 accgcattgg caactcgcca ccgcattgga atacggcaaa aaacgttatt ttgatttgcc
     5281 gatttataac ggcagctaca aacttgcctc tgccacattg ctttggttac gcaatccaca
     5341 acaatttttc tttgtcggca ctgatttcaa tcaagacaaa actcagttaa aacaatatag
     5401 ctctgatact aaaggcatac gctttggctg gcaacgagaa tggcaagccc tcgagctttc
     5461 cagtcgcctc acgcttggct atactgacag acgttacaaa gacaccgcta tattgggagg
     5521 aaaacttaat cttggcagaa tccgccatga ccgtgtgcaa agtgctaacc tcacgctttg
     5581 gaaacgagat tggcactggc tcggcctgat gccgaagctg caatttagtt acgccaaaca
     5641 ccgtagcaac ctgccgagcc tctatgccta caccgataaa aacatcaatt taattattga
     5701 aagtcggttc tagctccata aaaacaacaa gcggttaaaa ttcctagaaa ttttgcaaat
     5761 tgcaaaaaat gtagaaaatt taaccgcttg tatctatact gcagcttctt ttccttcagt
     5821 ataaattaat atacaccgtt gcaacaataa tggctgctcc ctgtcatcac cttgaactga
     5881 aaattgctca aggtggatat gactatcgca gccgcttcca ccgcacccag taatgtatgg
     5941 gcaagcaggt tggcttgggt atctactttt taatttgttc gttgagatat ggcgaagcta
     6001 agcctactgc tgcttgtcct gaatctttcg ccgctaaacc ttgtaaggct gccgtcaccg
     6061 catccaccgc acgcttggtt ttgctgcccg tgccgtaggc gttatcaaaa gtctgctgtt
     6121 tatcgtttaa atcccgtttt aattggagat attcaggatg ttgagcgatt tcttcttttg
     6181 tttttccctc atttctcaaa cgggtttcca ctttgcccaa ttccagcttc gcttccgtca
     6241 ctttttcacg ctcactatgc gtgtaaatct gataagcctt ctgcaatctc gcctatgccc
     6301 ttcaccagct cctgtgtttc tttcacctca ctaatatcgt gttctattaa ctctatctct
     6361 ttaggaactg ctccaatcat agacaatgga ataacttgat ctttattttc aatattcgga
     6421 tctgcccccg catttaataa tgctatcgct gcatcaacat tctttgccct cattgcataa
     6481 tgtaaagggg tcattccgta gcaatcttgg gcattaacat ctaccccatg tttaatgtaa
     6541 aattcgacta cagaaagagg agatggtgaa taactattta agttacacat atgtagccag
     6601 ttccattttt cttttggtgt gctatccgta atagtcagtt caccttttac gaaaaatgtt
     6661 tcttgtaaaa gatcgagatc gccttctgag tttgctgcaa taactcttaa ttcaggatct
     6721 tgatcatata attcttgata acgttctaat gtctgctgac gttctttatt cttatctctc
     6781 attttacttt cctatttgtt ggaatttttt catgtcatcg attattgatt taatattatc
     6841 tttccccggc atctcattct tatgactccg attttcaga
//
LOCUS       DANLYI010000133         6833 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.134, whole genome shotgun sequence.
ACCESSION   DANLYI010000133 DANLYI010000000
VERSION     DANLYI010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 6833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6833
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.134"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            52..1029
                     /gene="manX"
                     /locus_tag="PXD81_001826"
     CDS             52..1029
                     /gene="manX"
                     /locus_tag="PXD81_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005612905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HDL5537833.1"
                     /translation="MSIAIIIATHGVAAEQLLKTTEMLIGEQEDVAYIDFVPGENAET
                     IMGKYQALVEGPLSHCDQVLFLVDMWGGSPFNAANRFQDGKSGMDVVAGVNIPMLVNT
                     CMARDDVESLEELVEVALEAGSTGIRSLNHQPEVAEVQTATPAQETAAVQQPEIVDDG
                     RRMTIALARIDDRLIHGQVATVWTKESKVNRIIVVNEGVAKDSVRTTMLKSVAPLGVT
                     AHVVDVPKMIRVYNNPEYADDRVMLLFTNPSDVLALIEGGVPLNSINVGGISHKEGKQ
                     MVSHSVAVDQNDIDAFKAIDAKGVELEVRKVSSDSRQYMMDLLKKHNLI"
     gene            1041..1841
                     /locus_tag="PXD81_001827"
     CDS             1041..1841
                     /locus_tag="PXD81_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005716709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HDL5537834.1"
                     /translation="MELSALQIVLLFLVACISGMGSILDEWQTHRPLIACTMIGLVLG
                     DVTTGVIVGGSLELLALGWMNIGAAVAPDAALASIVSTILVIVGRQDISTGIAIAIPL
                     AAAGQVLTYVVRAITVGFQHAADKSIEDGNLSRLDWLHRGALLLQAMRIAIPALIVAL
                     TAGTDAVQTMLNAIPEVVTTGLKIAGGFIAVVGYAMVINMMRAGHLMPFFFAGFVIAG
                     FTDFNLVALGVLGTVIAMVYIHMHPKYNKSQQVVVAAAPKNDLDNRLD"
     gene            1855..2691
                     /locus_tag="PXD81_001828"
     CDS             1855..2691
                     /locus_tag="PXD81_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005564636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HDL5537835.1"
                     /translation="MTTEIKKVTKSDLNAVVRRSNLFQGSWNFERMQALGFAYAMVPV
                     IKRLYPDPNGPGRKEAIKRHSEFFNTQPFVGAPILGVTIAMEEERANGKEIDNAAING
                     IKVGLMGPLAGVGDPIFWGTVRPVFAALGAGLALKGELLGPILFFALFNIVRLAARYY
                     GVVYGYKKGLDVVQDMSGGLLQKLTEGASILGLFIMGALVQKWTSINVPLVLSTIQKQ
                     DGTIEVTTVQSILDSLMPGLLPLLLTFACMWLLRNRVNAIWIILGFFVIGILGAATGA
                     LA"
     gene            2784..3269
                     /locus_tag="PXD81_001829"
     CDS             2784..3269
                     /locus_tag="PXD81_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 domain-containing protein"
                     /protein_id="HDL5537836.1"
                     /translation="MTNTILILFILLGLVFAIYDQVFMHKLKGTTLLEIRLKKQKTID
                     TWLLIGLIVLSISYGVQNQIQPFTLYLLATCIILSVYLAFFRSPRLLLKQHGFFFANV
                     FFNYDKIYQVNIAEGKADEKILVIDLHSGRRLLAYVENAEDLQPVVDFFGGYKKESEQ
                     K"
     gene            complement(3355..4146)
                     /gene="tpiA"
                     /locus_tag="PXD81_001830"
     CDS             complement(3355..4146)
                     /gene="tpiA"
                     /locus_tag="PXD81_001830"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HDL5537837.1"
                     /translation="MARRPLVMGNWKLNGDKAFTKTLIADLKAELHGVEGCDVAIAPP
                     VMYLAHAEASLAGCGCGCSDKGLIALGSQNVDVNVKGAFTGDISPEMLKEFGAKYIII
                     GHSERRTYHKESDEFIAQKFKVLKDAGLVPVLCIGESEAENEAGKTEEVCARQIDAVI
                     NAHGVEAFNGAVIAYEPIWAIGTGKSATPAQAQAVHKFIRDHIAAKSQAVADQVIIQY
                     GGSVNDANAAELFTQPDIDGALVGGVSLKAPAFAVIVKAAAAAKA"
     gene            4306..5325
                     /gene="truD"
                     /locus_tag="PXD81_001831"
     CDS             4306..5325
                     /gene="truD"
                     /locus_tag="PXD81_001831"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HDL5537838.1"
                     /translation="MKLNYLYSPPQAVGRLKAEYADFIVREELGYELAGEGEFVAVKI
                     RKTNASTLFVGEKLAKFVGISEREMSYAGLKDRHTVTEQWFCLHLAGKETPDFSAFEC
                     EGVEVLEVTRHNRKIRVGSLAGNHFELLLRDVAESEELIYRLQQLQAVGFPNYFTEQR
                     FGRDGHNLTQALRWANGEISVKDRKKRSFYLSAARSEVFNLVVSQRINDCLTQKILEN
                     DYVQLAGSNSFFCVKSDEIAETQARLAQQDLLLTAPLIGERSLELECNPAEKAIIEQH
                     AQLVALMQKERMAAARRAMLCKPQNLEWQFEQEGLRLKFFLDSGSYATALVRELILLT
                     EESEA"
     gene            5496..6284
                     /gene="surE"
                     /locus_tag="PXD81_001832"
     CDS             5496..6284
                     /gene="surE"
                     /locus_tag="PXD81_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HDL5537839.1"
                     /translation="MRILISNDDGFHAVGIQTLAKALRDAGHIVTIIAPDRNRSAASS
                     CLTLVDPLRVHRFDEYNYAVIAGTPADCVHLALNGLFDGKFAEKFDLVVSGINHGANL
                     GDDVVYSGTVAAALEGRHLPLPSIAVSLVGRKHSSFLDGENHFGTAAQVVLDLLEKME
                     TQRFPSNHVLNVNVPNLPYSELKGTMITRLGERSPAAEIVKQKDPRNADVYWIGVTGE
                     PLDESFGTDFYALNRDCVSITPIQVDMTAHKTIATLKGIFNETV"
     gene            6271..>6833
                     /locus_tag="PXD81_001833"
     CDS             6271..>6833
                     /locus_tag="PXD81_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HDL5537840.1"
                     /translation="MKLFETIYDKTMQWSKHRFAAFWLSFVSFIEAIFFPIPPDVMLI
                     PMSMAKPQNAFKYAVYTTLASVAGGVIGYFIGLYATDWVQGVIANWGMQANFDKAKLW
                     FETWGVAVVFLAGFSPIPYKVFTICAGVMQMAFFPFVITAAISRFARFILVAKLSAWG
                     GKKYEQKIRRSIELIGWGTIVLAVVAY"
BASE COUNT         1891 a         1385 c         1519 g         2038 t
ORIGIN      
        1 aaaatctcgc cccagtaaat aaacaactta aaaaaccgca aggagtaaca aatgagcatt
       61 gctattatca tagcaaccca tggtgttgct gcagagcaac ttctcaaaac aactgagatg
      121 ttgattggtg aacaagaaga tgttgcttac attgactttg ttcccggcga aaatgcagaa
      181 acgattatgg gcaaatatca agcattggtt gaaggtccac tatcacactg tgatcaagtg
      241 ctgtttttgg tggatatgtg gggaggaagc ccattcaacg ccgcaaaccg attccaagat
      301 ggaaaatcgg gtatggatgt tgttgcgggt gtgaatattc ctatgttagt gaacacctgt
      361 atggctcgtg atgatgtgga gtcgttggaa gaattggtgg aagtcgcatt agaagccggc
      421 tcaaccggga ttcgttcact taatcaccaa cctgaagtgg ctgaggtgca aaccgctact
      481 cctgcacaag aaactgcagc tgttcaacag cctgaaattg ttgatgacgg acgtcgtatg
      541 accattgccc ttgcccgtat tgatgaccgc ttgattcacg gtcaagttgc gacggtttgg
      601 acaaaagaaa gtaaagtcaa tcgcattatt gtggtaaacg aaggtgtagc aaaagatagt
      661 gtacgaacta ctatgctaaa aagtgttgct cctcttggcg taacggctca cgttgttgat
      721 gtacctaaaa tgattcgtgt ctataacaac cctgaatatg ccgatgatcg tgtaatgtta
      781 ctctttacca acccaagtga tgtcttagcc ttaattgaag gcggtgtacc actcaatagc
      841 attaacgtgg gcggtatttc gcataaagaa gggaaacaaa tggttagcca ttctgtggcg
      901 gttgatcaaa atgatattga tgccttcaaa gcaattgatg cgaaaggcgt tgaattagaa
      961 gtacgcaaag tctcaagcga tagccgtcag tatatgatgg atttattgaa aaaacataac
     1021 ttaatctaaa ggaaattact atggaacttt cagcattaca aattgtgctt ttattcctcg
     1081 ttgcctgtat ctcaggtatg ggctcaattc ttgatgaatg gcaaacccac cgtccgttaa
     1141 ttgcctgtac gatgataggc ttagtgcttg gtgatgtgac taccggcgtt attgttggtg
     1201 gctcattaga gttattagct ttaggttgga tgaacatcgg tgccgctgtt gcaccagatg
     1261 cggcattagc ctctattgtc tcaaccattt tagtcattgt aggtcgtcaa gatatttcaa
     1321 ctggtatcgc catcgctatt ccgcttgcag ctgccggtca agtattaacc tatgttgtgc
     1381 gtgcaatcac cgtaggtttc caacacgcag cagataaatc aattgaagac ggtaacttaa
     1441 gccgtttaga ctggttacat cgtggtgctt tattgcttca agcaatgcgt attgccattc
     1501 cggcattaat tgtggcatta acagcaggta cagatgcggt tcaaacaatg ttaaatgcca
     1561 ttcctgaagt ggttacaacc gggctgaaaa ttgccggtgg cttcatcgcc gtggtgggtt
     1621 atgcgatggt gatcaatatg atgcgtgcag gtcatttaat gccgttcttc ttcgcaggct
     1681 ttgtaattgc aggctttacc gatttcaatc ttgttgccct tggggtatta ggtacggtta
     1741 ttgcaatggt ttatatccat atgcacccta aatataacaa aagccaacag gtagtggtgg
     1801 cagctgcacc aaaaaatgat ttagacaacc gtttagacta agagggcatt taaaatgaca
     1861 actgaaatca aaaaagtaac aaaaagcgat ttgaatgccg ttgtacgtcg ttcaaactta
     1921 ttccaaggtt cgtggaactt tgagcgtatg caggctcttg gttttgcgta tgcgatggta
     1981 ccggttatca aacgtttata tccggatcca aatggcccgg gtcgcaaaga agcaattaaa
     2041 cgccattctg agttctttaa cacccagcca ttcgtcggtg caccgatttt aggggtaacc
     2101 attgctatgg aagaagaacg tgccaacggt aaagaaatcg acaatgccgc aattaacggt
     2161 attaaagttg gtttaatggg accattagcc ggtgtgggtg acccaatttt ctggggaact
     2221 gttcgccctg tatttgccgc attaggagca ggcttagcct taaaaggtga gcttttaggc
     2281 ccaatcctgt ttttcgcctt atttaacata gtgcgtttag ccgcacgcta ttatggcgta
     2341 gtgtatggct ataaaaaagg gttagatgtt gtacaagata tgagcggcgg tttattgcaa
     2401 aaattaaccg aaggtgcttc aatcttaggt ctgtttatta tgggggcatt ggtacaaaaa
     2461 tggacgagca ttaacgttcc gcttgtgctt tcaaccattc aaaaacaaga cggtaccatc
     2521 gaagttacca cagtgcaaag cattctcgac agcttaatgc cgggcctact cccattatta
     2581 ttaacctttg cttgtatgtg gttacttcgt aatcgagtaa atgctatctg gattatcctc
     2641 ggtttctttg ttatcggtat cctcggcgct gcaaccggtg cattagctta attgcaaaat
     2701 tttctgtgaa aacgaccgct tgaacttgaa cattcaagcg gttttttata cgctttagcc
     2761 caaataaatt cacttgctta attatgacca ataccatcct tattctcttt attttacttg
     2821 gcttagtatt cgccatttat gaccaggttt ttatgcacaa actcaaaggc acaaccttac
     2881 tggaaatccg tttaaaaaag caaaaaacaa ttgatacttg gttattgatt ggtttaatcg
     2941 tcttgtcaat tagctatggc gtacaaaatc aaatccagcc ttttacactt tacctgctgg
     3001 cgacttgcat tattctgtcg gtctatctcg cctttttccg ttcccctcgc ttgttgttaa
     3061 aacagcacgg ttttttcttt gcgaatgtct tttttaatta cgacaagatt tatcaagtca
     3121 atattgcaga aggaaaagca gacgaaaaaa ttttggtaat tgatttacat agcggaagaa
     3181 ggctgcttgc ctatgttgaa aatgcggaag atcttcaacc ggtagtggat ttttttggcg
     3241 gttataaaaa ggaaagtgag cagaaatagt ttgcgaacaa aacacaagcg gtcatttttc
     3301 cctgaaattt tgcaaaaaac tgaggaaaaa tgaccgcttg taaaatcggt taaattaggc
     3361 tttagcagcc gctgccgctt taacgatcac cgcaaaagca ggcgctttaa gtgaaacacc
     3421 gcctactaat gcaccgtcaa tatccggttg agtgaataac tctgctgcat tggcatcgtt
     3481 tacagagcca ccgtattgaa tgatcacttg gtcagccact gcttgcgatt tagctgcaat
     3541 atggtcacga ataaatttat gcactgcttg tgcttgtgcc ggagtcgctg atttgccggt
     3601 accgattgcc caaattggtt cgtaagcaat aactgcaccg ttgaatgctt ccacaccgtg
     3661 cgcgttgatg accgcatcaa tttgtcttgc acacacttct tcagttttac ctgcttcatt
     3721 ttcggcttca gattcaccga tacataatac aggcactaag ccggcatctt ttaatacttt
     3781 gaatttttga gcaataaatt catcgctttc tttgtggtaa gtacggcgtt ctgaatgccc
     3841 gataataata tattttgccc caaactcttt taacatttca ggtgaaatat caccggtgaa
     3901 tgcccctttt acgttcacat caacgttttg tgaaccaagt gcgattaagc ctttatcact
     3961 gcaaccacag ccacaaccgg caagtgatgc ttcagcgtgt gccaaataca tcaccggtgg
     4021 agcgattgct acatcacagc cttcaacgcc atgtaattct gcttttaaat ctgcaattaa
     4081 ggttttagta aacgctttat caccgtttaa tttccagtta cccattacaa gtggacgacg
     4141 tgccattttg tttctcctat atgaagttga acttaaatat aaacgttgca aactatacca
     4201 aattttaatg tcgttttgtt gtttttattt tgctttttct gatctagatc gaaaggttta
     4261 acgtataatg ccgaaatctt attttttatg ttattcgaga agtgaatgaa actgaattat
     4321 ttatactctc ctcctcaagc tgttgggcga ctaaaagccg aatatgcaga ctttattgtg
     4381 cgtgaagaac tgggctacga actagccggc gaaggggaat ttgtggcggt taaaatccgc
     4441 aaaaccaacg ccagcacgct gtttgtgggg gaaaagttag ccaaatttgt cggtatttcc
     4501 gagcgtgaaa tgagttatgc aggcttaaag gaccgccaca ccgtgactga gcaatggttc
     4561 tgcttacact tggcaggcaa agaaacgccc gatttctccg cctttgaatg cgaaggcgtg
     4621 gaagtgctgg aagtgacccg ccacaaccgc aaaattcgtg ttggttcgct cgccggcaat
     4681 cattttgaat tattgctacg tgatgtagca gaaagcgaag agttgattta ccgtttgcaa
     4741 cagttgcaag cggtcggatt tcccaattat tttaccgaac aacgtttcgg gcgagacgga
     4801 cataacctga ctcaagcctt gcgttgggca aacggggaaa ttagcgtgaa agatcgcaaa
     4861 aaacgcagtt tctacctttc cgctgcccgc tcggaagtgt ttaatttagt ggtttcacag
     4921 cggattaatg actgcttaac ccaaaaaatc ttggagaatg attacgttca acttgccggc
     4981 tctaacagct ttttctgcgt gaaatcggac gaaattgctg aaactcaagc ccgtttagca
     5041 cagcaagatc tacttctcac cgctccgctg ataggcgaaa gatcgcttga attagaatgc
     5101 aatcctgctg aaaaagccat catagagcaa cacgctcaat tggttgcctt aatgcaaaaa
     5161 gagcgtatgg cagccgctcg gcgagcaatg ctttgtaagc cacaaaatct ggaatggcaa
     5221 tttgaacaag aagggctacg gttaaaattc tttttagatt caggcagtta tgccactgca
     5281 ctggtgcgag agttaattct gcttaccgaa gagagcgaag cttagtccat aacacataac
     5341 aagcggtaag attttaatca ttttttgcaa tttgcaaaac tttaacaaaa tcttaccgct
     5401 ttttgcactt ttagactaaa aaaactgtcg aaaaattgtt agaattgcga tctttgtatt
     5461 tagagtctat caagatgtaa gatctaaagg aaattatgcg aattttaatt agtaatgacg
     5521 atggttttca cgctgtcggc attcaaactt tagccaaagc cttgcgagat gcaggccata
     5581 tcgtcaccat tattgcaccg gatcgtaacc gcagtgcagc ttcaagctgc ctaaccttag
     5641 tagatccact tcgggtgcat cgttttgatg aatataatta tgcggtgatc gccggcacac
     5701 ctgccgattg cgtgcattta gccttaaacg gcttgtttga cggcaaattt gcagaaaaat
     5761 ttgatttggt ggtctccggc attaatcacg gggcaaattt aggcgatgat gtggtttact
     5821 ccggtacggt agcggcagca ttggaaggcc gccacttgcc attaccaagt attgcagtat
     5881 cactggttgg gcgtaaacac tcttcgtttt tagacggtga aaatcatttt ggaacggcag
     5941 cacaggtagt gttagattta ttggaaaaaa tggaaacgca acgttttcca tcaaaccacg
     6001 ttctcaatgt taatgtgccg aatttacctt attcagagct aaaaggcaca atgatcaccc
     6061 gcttaggtga acgttcaccc gctgcggaaa ttgtgaagca aaaagatcct cgtaatgctg
     6121 atgtctattg gattggtgta acaggtgaac cactggatga aagttttggt actgattttt
     6181 atgcattaaa ccgagattgc gtttctatca caccaattca agtggatatg accgctcata
     6241 aaacgatagc aaccttaaaa ggcattttta atgaaactgt ttgaaacaat ttatgacaaa
     6301 acaatgcagt ggtctaagca ccgcttcgct gccttttggt tgagttttgt cagttttatc
     6361 gaggcgatct ttttcccgat tccgccggat gtgatgttga ttccgatgtc gatggcaaaa
     6421 ccgcaaaatg cgtttaaata tgcagtttac actacccttg cttcggttgc cggtggtgtt
     6481 attggttatt ttattggctt atatgctacc gattgggtgc aaggcgtcat cgcaaattgg
     6541 ggaatgcaag caaactttga taaagcaaaa ttgtggtttg aaacttgggg tgtcgcggta
     6601 gtatttttag ccggtttttc gccgattccc tataaggtat ttaccatctg tgccggtgta
     6661 atgcagatgg cgttcttccc gtttgtgatt actgctgcga tttctcgctt tgcccgtttt
     6721 attttggtag caaaactctc tgcttggggt ggcaaaaaat atgaacaaaa aattcgccgc
     6781 tcaattgaat taattggttg gggaacaatc gttttagccg ttgttgccta tat
//
LOCUS       DANLYI010000134         6786 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.135, whole genome shotgun sequence.
ACCESSION   DANLYI010000134 DANLYI010000000
VERSION     DANLYI010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 6786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6786
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.135"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            <1..2568
                     /gene="topA"
                     /locus_tag="PXD81_001834"
     CDS             <1..2568
                     /gene="topA"
                     /locus_tag="PXD81_001834"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HDL5537841.1"
                     /translation="AKKINQYLGSNYVVKSSVGHIRDLPKSGSDEKTEKAKPISTKGL
                     TAAEKAKIKAEKERVALVKRMGIDPYNGWKANYQILPDKEKVVSELKSLAKKADHIYL
                     ATDLDREGEAIAWHLREIIGGDDSRFSRVVFNEITKDAIKKAFDDPQPLNIDRVNSQQ
                     TRRFLDRVVGFMVSPLLWKKVARGLSAGRVQSVAVKLLVEREREIKAFQSEEFWTILA
                     NTQINRKKLPLELTTYKGKKFSASNEAEANVAVRALEQSDFIVSNIEAKETSSRAAAP
                     FITSSLQRAASTRLGFGVKKTMMLAQRLYEAGYITYMRTDSTNLSREALAMARGYIQD
                     NFGANYLPEKANVYRSKDNAQEAHEAIRPSDVNVKMADLKGMDKDAERLYDLIWRRFL
                     ACQMLPARYDSTAVTVSAGDYELKANGRVLRFDGWTKVLPVQGKNPEDQELPAVELHQ
                     KLTLDEIVPSQHFTKPPARFNEASLVAELEERKIGRPSTYASIISTIQERGYVRTENK
                     RFYVEKIGEVVTDRLNESFKDLMNYDFTANMEEVLDQIAEGSKNWKEELNTFFKHFSE
                     ELSQAELDELEGGMKPNSLVPTDIDCPTCGRKMAIRTASTGVFLGCTGYALPPKERCK
                     TTMNLIPEAEFFNVLDSDSETKALMARKRCPKCDSAMDSYVIDAERKLHVCGNNPNCD
                     GHIIEQGSFKIKGYDGPVVECDKCGSDMHLKLGRFGKYMGCTSCDNTRKILKNGEVAP
                     PKEDPIAFPELKCEKADAYFVLRDGASGVFMSAHNFPKVRESRAPKVVELAQYRERLP
                     EKLQYLADAPQQDPEGNEAIIRFSRKEKRQYVTSEKNGKATKWIVDYLDGKWVERKK"
     gene            complement(2618..3556)
                     /gene="yvcK"
                     /locus_tag="PXD81_001835"
     CDS             complement(2618..3556)
                     /gene="yvcK"
                     /locus_tag="PXD81_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HDL5537842.1"
                     /translation="MENTDIIPFKPTRHHYLNQLNAVVALGGGHGLGRLLSALSFLKE
                     RLTGIVATTDNGGSTGRIRSQQGVIAWGDLRNCLNQIIVKPTIASRLFEYRFAGTGEL
                     AGHNLGNLILTALENMQIRPLEGINLVRELLHVRSQLIPMSEIPVHLIAQLHSGESVF
                     GEVQIDSLAETPQHLALDTMVVATPEAISAIQSAELILLGPGSFFTSIMPNLLVAGIA
                     NSIAQSKARVVFIDNIGQEHGPAGQLSLNQRIEWMENCIGQARINAVITTDDAGSELP
                     EHIQILRKNLSANDVYYRHDRNKLSKAIDELLAQNI"
     gene            complement(3570..4646)
                     /locus_tag="PXD81_001836"
     CDS             complement(3570..4646)
                     /locus_tag="PXD81_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDL5537843.1"
                     /translation="MFQMLQDWYRKKFTDPQIVVLFSILLIGFGIIYFFSDLLMPLLV
                     ALVFAYLLEWPIRFLSSKLKLPRTLSVILVLGGFIALLSFLGVVLLPSLWNQAVTFIQ
                     DLPSMFNLLNAWLQALPEHYPELVDYATLDSIVNTAKSNILSMGESLLTLSINSIISL
                     VGLGIYTFLVPLMVFFLLKDKIVLMRSFSKVLPQNRRLATRVWFEMQQQIANYIRGKF
                     LEILIVGVVTYIIFLFFDLRYPLLLSVAVGISVLVPYIGAVLVSIPVMLIALFQFGLS
                     PDFYYLMLAFIISQLLDGNLLVPFLFSEAVNLHPLTIIIAVLIFGGLWGFWGVFFAIP
                     LATLVKAVLQALPSAQSDAIILEK"
     gene            complement(4677..5432)
                     /locus_tag="PXD81_001837"
     CDS             complement(4677..5432)
                     /locus_tag="PXD81_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009874881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDL5537844.1"
                     /translation="MKKEEVGHHFLARLGKTRLRPGGKLATDWLIANGDFDKNKKVLE
                     VACNMGTTAIQLAQQFECQIVGIDLDEDALEKARKNIKENHVEHLIQVQRANATKLPF
                     DDESFDIVINEAMLTMLPLEAKEKAIREYFRVLKPNGFLLTHDIVIQDKDSDTLLAEL
                     RDAINITVSPLAKADWKSLFQRCGFRNVDTFSGEMSLLSPKGLIHDEGVLGAMKIIGN
                     ALKPENRETFNKMYKLFNDPDKKLGFIAVCSQK"
     gene            5584..5793
                     /locus_tag="PXD81_001838"
     CDS             5584..5793
                     /locus_tag="PXD81_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome alternative rescue factor ArfA"
                     /protein_id="HDL5537845.1"
                     /translation="MSKQKNDYLHQRGQIKESAVKALVTDPLFRQRVERNRKGKGSYR
                     RQEKHKKTIYGQKETPFKNIRLESF"
     gene            complement(5842..6564)
                     /gene="bioD"
                     /locus_tag="PXD81_001839"
     CDS             complement(5842..6564)
                     /gene="bioD"
                     /locus_tag="PXD81_001839"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HDL5537846.1"
                     /translation="MPSLFITGTDTNVGKTVVTRAIIQTLTKHSFPVIGYKPIACGGD
                     DSLPTEPTQYDYATEDNSDVLTILDSCPTNVSYRDINSYTFIHSSTPVFAALDAVHHI
                     QLEKLNTDLKRLENAYPNVVVEGTYGWLTPINKDYYFADWVMQNNMPVVLVVGIKEGC
                     VNHALLTANEILQRGVKLVSWVANRVNPGLRHYHELIELLTQKINAPLLGQIPYMGHP
                     EHKELSSYIQNPEPLLAYFSKP"
BASE COUNT         2144 a         1257 c         1433 g         1952 t
ORIGIN      
        1 gcaaaaaaaa ttaaccaata tcttggtagt aattatgtgg tgaaatcgag tgtggggcat
       61 attcgtgatt taccgaaaag tggtagtgat gaaaaaacgg aaaaagcgaa accgatttca
      121 accaaagggc tgaccgctgc cgaaaaagct aaaatcaaag cagaaaaaga gcgtgttgca
      181 ttggttaaac gtatgggtat cgacccctat aatggctgga aagctaacta tcagattttg
      241 ccggataaag agaaagtggt ttccgagcta aaatcgttgg caaagaaagc agatcatatc
      301 tatctggcaa ccgacttgga tagagaaggg gaggcgattg cgtggcattt gcgtgaaatc
      361 attggtggtg atgacagccg ttttagccga gtggtattta atgaaatcac caaagatgcg
      421 atcaaaaagg cttttgacga tccgcaaccg ctcaatattg accgtgtaaa ttcccagcaa
      481 acccgccgct ttttagaccg tgtagtgggc tttatggtgt cgccattatt atggaaaaaa
      541 gtagctcgtg gtttatcggc agggcgtgtg caatcggttg ccgtaaaatt actggttgag
      601 cgtgagcgtg aaatcaaggc gttccaaagc gaggagtttt ggactattct tgccaatacg
      661 caaattaatc gtaaaaaatt accgcttgaa ttaactactt ataaaggtaa aaaattctcg
      721 gcaagcaatg aggctgaagc aaatgttgca gtgcgtgctt tggagcagag cgattttatt
      781 gtttctaata ttgaagccaa agaaaccagc tctcgagctg ctgcaccatt tattacctca
      841 tcgttacaac gtgccgctag cactcgttta ggttttgggg ttaaaaagac gatgatgtta
      901 gcccaacggt tatatgaagc gggttacatc acctatatgc gtactgattc aaccaacctt
      961 agccgtgaag cattagcaat ggctcgaggt tatattcagg ataatttcgg ggcgaattat
     1021 ttgccggaaa aagcaaatgt gtatcgcagt aaagataatg cccaagaagc tcacgaagcc
     1081 attcgcccgt cggatgtgaa tgtcaaaatg gcggatttaa aaggaatgga taaagatgcc
     1141 gagcgtttat acgatttaat ttggcgtcgt ttcttggcgt gtcaaatgtt gcctgctcgt
     1201 tatgattcta ccgcagtcac agtgtctgcc ggagactatg aactcaaagc aaacgggcgt
     1261 gtactgcgtt ttgacggctg gacgaaagta ctgccggtgc aaggcaaaaa tccggaagat
     1321 caggaattac cggcggttga actgcatcaa aaattaacgc ttgatgaaat tgtgccaagt
     1381 cagcatttta ctaaaccgcc tgcacgtttt aatgaggcgt ctttggttgc tgagttagaa
     1441 gagcgaaaaa ttggccgccc ttcaacctat gcttcaatta tttcgacgat tcaagaacgt
     1501 ggctatgttc gcaccgaaaa caagcgtttt tatgtggaaa aaatcggtga agtagtgacg
     1561 gatcgcctca acgaatcatt caaagattta atgaattatg atttcactgc caatatggaa
     1621 gaggtgctcg accaaattgc agaggggtct aaaaattgga aagaagagtt gaatactttc
     1681 tttaaacatt tctctgaaga gttgagccaa gcagaattag atgaacttga aggcggaatg
     1741 aaaccaaaca gccttgtgcc aaccgatatt gattgcccga cttgtggacg aaaaatggca
     1801 attcgcaccg cttccacagg ggtattttta ggttgcaccg gctatgcttt accgccaaaa
     1861 gagcgttgca aaacgacaat gaatttaatt ccagaggcgg aatttttcaa tgtgttagat
     1921 tcggattctg aaaccaaagc cctaatggct cgcaagcgtt gcccgaaatg cgattcggca
     1981 atggatagtt atgtgattga tgctgaacgt aaattacacg tttgtggtaa taatccaaat
     2041 tgtgatggtc atattattga gcaaggttcc ttcaaaatta aaggctatga cggacctgta
     2101 gtggaatgcg ataaatgcgg ttcagatatg cacttaaaac tgggtcgttt cggcaaatat
     2161 atgggctgca caagctgcga taacacccgt aaaattctga aaaacgggga agttgctccg
     2221 ccaaaagaag atcctattgc ttttcctgaa ttaaaatgtg aaaaagccga tgcctatttt
     2281 gtattaagag acggagcaag tggggtgttt atgtcagctc ataacttccc gaaagtgcgt
     2341 gaaagccgtg caccaaaagt ggtagagtta gctcaatatc gtgaacgttt gccggagaaa
     2401 ttgcagtatc ttgccgatgc tccgcagcaa gatccggaag gaaacgaggc aattatccgt
     2461 tttagtcgta aagagaaacg ccaatatgtg acttctgaga aaaacggcaa agccaccaaa
     2521 tggattgttg attatctaga cggtaaatgg gttgagcgta agaaataaag cttaaaagac
     2581 aagcggtaaa attttctaaa aattttaccg cttgctttta gatgttctga gcgagtagtt
     2641 catcaattgc tttacttaat ttattgcgat cgtgtcggta ataaacgtca ttcgcagata
     2701 agttttttcg caagatttga atatgttcgg gaagttcaga accggcatca tcagtcgtaa
     2761 ttaccgcatt aattctagcc tgtccgatac agttctccat ccattcaatt cgctgattaa
     2821 gtgatagttg cccagctggg ccatgttctt gtccgatatt gtcaataaat actactcggg
     2881 ctttgctttg agcaatagag tttgctatgc ctgccaccag caaattcggc ataatactgg
     2941 taaaaaagct acctgggcct agcagtatta actcagcact ttgaatagct gaaatggctt
     3001 ctggtgtagc aacaaccata gtatctaacg ctaaatgttg tggtgtttct gctaaagaat
     3061 caatttgcac ttcaccaaat actgattcgc ctgaatgcaa ctgtgcaatt aaatgcaccg
     3121 gtatttcaga cattgggata agttgcgatc ttacgtgcaa taattctcgc accaaattaa
     3181 tcccttctag cggtcggatt tgcatatttt caagggcggt gagaattaaa ttgcctagat
     3241 tatgtccagc gagttcgcct gtgccagcaa aacgatactc aaacaaccga gaagctatcg
     3301 ttggtttaac gataatttga tttaaacagt tgcgtaaatc tccccaagca atcacccctt
     3361 gttgtgaacg tattctaccg gttgagcctc cattatcggt agtggcaaca atgccggtaa
     3421 gccgttcctt taaaaaagag agcgcggaga gcaatcgacc taagccgtgg cccccaccta
     3481 gtgcaacaac tgcattgagt tggtttaaat agtgatgtcg ggtaggttta aaaggaataa
     3541 tatctgtatt ttccatcttg ggatttcgtt tatttttcta aaataatggc atctgactgg
     3601 gctgaaggca atgcttgtaa tacagcttta accaaagtcg ctaaaggaat cgcaaaaaac
     3661 actccccaaa agccccataa accaccaaag atgagtacgg caataataat tgtcagaggg
     3721 tgtagattta ctgcttcaga aaataaaaag ggtacgagta aatttccgtc tagcaactga
     3781 ctaataataa atgcgagcat taaatagtag aaatccggcg ataacccgaa ctggaataga
     3841 gcaattaaca ttaccggaat gctgactaaa accgcaccga tgtagggaac taacactgaa
     3901 atccctaccg ctacagaaag tagtaacgga tagcgtaaat caaaaaagag gaaaataata
     3961 taagtgacaa ccccaacaat tagaatttct aaaaatttac ctcgaatata gttggcaatt
     4021 tgttgttgca tttcaaacca tactcgtgtt gcaagtcgac ggttttgcgg taatacttta
     4081 ctaaaagaac gcattaaaac gattttatct ttcagtaaga aaaagaccat gagtgggact
     4141 aagaaggtgt aaatgcctaa gccaactaag ctgatgatag agttaatcga gagtgttaga
     4201 agtgattctc ccatactcaa aatattactt ttagcggtat tcacaatgga atctagggtc
     4261 gcataatcta ctaattcagg gtaatgctcc ggtagggctt gtaaccaagc attgagcaga
     4321 ttaaacatcg aaggtaaatc ttgaataaag gtaactgctt gattccaaag gctgggtagt
     4381 agtactacgc ctaaaaaaga taataaagcg atgaaacctc ctagtactag aatgacactc
     4441 aaagttcgag gtaatttgag tttgctcgac aaaaaacgga ttggccattc caataaatag
     4501 gcaaacacaa gtgcaaccag aaggggcata agcagatcgc taaaaaaata gataataccg
     4561 aaaccgatca atagaatact aaacagcaca actatttgcg ggtcggtaaa tttttttcga
     4621 taccaatctt gcaacatttg aaacatatgg attgccttta aattatgcga tgaaaattat
     4681 ttctggctac agactgcaat gaaccctagc tttttgtccg ggtcattaaa tagtttgtac
     4741 attttattaa aggtttcgcg attttctggt tttaatgcat tgccgataat tttcatggcg
     4801 cctaaaacac cttcatcatg aatcagacct ttaggggaaa gtagagacat ttctccggaa
     4861 aaggtatcaa cattacggaa accacaacgt tggaataatg atttccagtc tgctttagca
     4921 agtggagaga cagtgatatt gattgcatca cgcaactcgg ctaataaagt atcggagtct
     4981 ttatcttgga taacaatatc gtgcgttaat aaaaagccat ttggttttaa tacacgaaaa
     5041 tattcacgta ttgccttttc tttcgcttct agaggaagca ttgtcagcat ggcttcatta
     5101 attacaatat caaaactttc gtcgtcaaat ggcagttttg ttgcgttggc gcgctgtact
     5161 tgaattaaat gctcaacatg gttttctttg atatttttac gtgctttttc aagcgcatct
     5221 tcgtctaaat caataccaac aatttggcat tcaaattgtt gagctaattg gattgcagta
     5281 gtccccatgt tacaggcaac ttcgagcact tttttatttt tatcaaaatc cccgtttgca
     5341 attaaccaat cagttgctaa tttccccccg gggcgtaaac gagttttgcc taaacgagct
     5401 aaaaaatgat gaccaacttc ttcttttttc ataaaaacct ctggatttgc aaaatacgtt
     5461 aaatatatga ccgcttgtat tgtgatctat gttcgttatt atactcaatt ttttcatttt
     5521 cttatataca acatcaacag attttgccag aatacaacct cattttgata tgaggaaaat
     5581 agaatgagta agcaaaagaa tgattacctc caccagcgag gtcaaattaa agaaagtgca
     5641 gtaaaagcat tagtgactga tccacttttt agacaacgtg tagaaagaaa tcgtaaaggg
     5701 aaagggagct atcgcagaca agaaaaacac aaaaaaacta tctatgggca aaaagaaacc
     5761 ccatttaaaa atattcggtt agagtctttt taaatggggt tttatttgta gcttattgta
     5821 gttttataag cctgataaac actatggttt actgaaatag gccaacaacg gttctggatt
     5881 ttgaatataa gaagaaagtt ctttatgctc tgggtgaccc atatagggaa tttgcccaag
     5941 taaaggcgca ttaatttttt gtgtcagtag ttcaattaat tcgtgataat gccgtagccc
     6001 tggatttact ctgtttgcga cccaacttac taattttaca ccacgctgca aaatttcatt
     6061 cgcagtaagt agtgcatggt taacacaacc ttccttaatt cccaccacta aaacaaccgg
     6121 catattgttt tgcattaccc aatcagcaaa atagtaatcc ttgttaatag gagttagcca
     6181 gccataggtt ccttctacga caacatttgg ataggcattt tctagtcgtt ttaaatctgt
     6241 gtttaatttt tccagttgaa tatgatgaac tgcatctaaa gctgcaaata caggggtact
     6301 ggaatgaata aaagtatagc tattaatatc acgatagcta acattagttg gacagctatc
     6361 tagtatggtt aatacgtctg aattatcttc agtagcataa tcatattgag taggttctgt
     6421 gggtaatgaa tcatcaccgc cacaagcaat gggtttatag ccaattacag gaaaactatg
     6481 ttttgtaagt gtctgtatga tggctcgggt aaccacagtt ttccctacgt tggtgtctgt
     6541 tccggtaata aataatgagg gcataaatgg ctcctttcga aaatatggct ggtagtttaa
     6601 ccaaaaatag atcaatttat tctgtctttg atcaaatttc cgtcataaat tccttgtttt
     6661 attgctgaac aggcaattaa tggactattc caatcgtatt ggctggtaac taaatcgatt
     6721 tttaggtttg tttgtaacaa atttttatgc aatttttctg aaatttgcgt aaataacggc
     6781 tctttt
//
LOCUS       DANLYI010000135         6752 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.136, whole genome shotgun sequence.
ACCESSION   DANLYI010000135 DANLYI010000000
VERSION     DANLYI010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 6752)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6752
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.136"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            <1..400
                     /locus_tag="PXD81_001840"
     CDS             <1..400
                     /locus_tag="PXD81_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HDL5537847.1"
                     /translation="NNIIFIIKQNIKNPESYSINGAVGLLASALENLIRNGQKYAKKM
                     LSVSMNVEDGHLFLTVEDDGEGVPENEYENILKPFYRVDEARARETGGAGLGLAIVQN
                     VVKQHQGTIHLDKSNMGGLKIELKLPLWRS"
     gene            450..2372
                     /locus_tag="PXD81_001841"
     CDS             450..2372
                     /locus_tag="PXD81_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011200304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MacB family efflux pump subunit"
                     /protein_id="HDL5537848.1"
                     /translation="MKLIEIENLNKYFGLGQTRAHILKNINLSIERGDFVAIIGASGS
                     GKSTLMNIIGCLDTASSGICRIDGKETQTMSADELSDLRQRKLGFIFQRYNLLSALNA
                     NENVALPAIYAGMDSSSRKKRADTLLTKLGLSDKTQHKPNELSGGQQQRVSIARALMN
                     GGEIILADEPTGALDSKSGETVLEILRDLHYEGHTIIMVTHDPNIAAQASRIVEIKDG
                     QIIRDERQRTDVQETALNKTTTQKPRFWNQLFESFKMATSAIMAHKMRAILTMLGIII
                     GITSVISVVALGRGSQQQILSNINNLGTNTMDIMNGTGFGDRRANLTKNLTISDALML
                     SRQNYVESTTPSSNVSATLIYGNTAVTGSVRGVGEQFIDVKGLKLISGRFFSAEEVKN
                     VAQVIVIDPNTQKDLGLTEPVEGKTILVDKKPLQIIGVAQQSNNMNQSSLTLWAPYTT
                     IMQRISGAKQIDSLTVKIKDDIESQTAEKSIAELLVAKHGKKDFFIMNSDTIKQTITS
                     TTNTMTLLISSIALISLIVGGIGVMNIMLVSVTERTREIGVRMAIGAKQRNILQQFLI
                     EAVVICLIGGVIGILLASAIIWAFNTLGTNFKMMLSPESVIIAVLCSTLIGVVFGYIP
                     ARNAAQLNPITALAQE"
     gene            2456..3226
                     /locus_tag="PXD81_001842"
     CDS             2456..3226
                     /locus_tag="PXD81_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005604026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HDL5537849.1"
                     /translation="MTITNTELEQILNTKLNSSAINDYAPNGLQVEGKREIKKIITGV
                     TATLPLIEKAIEKNADAILVHHGYFWKSENPCIRGMKGKRIKQLLVNDINLFGYHLPL
                     DIHSELGNNAQLAKHLGVKNLQGLEDRPNSIPMYGELENPISANELKQKLEKTLQRTV
                     ILCDEFVHSPQKMIKTIGICSGGGQGYIDLAFEKGCDAFISGEISEQTTHSAREQGIY
                     YFACGHHATERDGVKALGEWLAVEYGLDVEFIDIDNPA"
     gene            complement(3307..4437)
                     /gene="nrdB"
                     /locus_tag="PXD81_001843"
     CDS             complement(3307..4437)
                     /gene="nrdB"
                     /locus_tag="PXD81_001843"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HDL5537850.1"
                     /translation="MAYTTFSQHKNDQLKEPMFFGQNVNVARYDQQKYEIFEKLIEKQ
                     LSFFWRPEEVDVSQDRIDYAQLPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLVSI
                     PELETWIETWTFSETIHSRSYTHIIRNIVNDPSIVFDDIVTNEEIIKRAKDISAYYDD
                     LIRDSQLYSLYGEGSYQVDGKTCEVSLRSLKRQLYLCMMSVNALEAIRFYVSFACSFA
                     FAERQLMEGNAKIIKFIARDEALHLTGTQHILNIMAYGNDDPEMAEIARECRQEAYEL
                     FLSAAEQEKAWAEYLFKDGSMIGLNRDILVQYIEYITNIRMQAVGLPLPFKARSNPIP
                     WINAWLVSDNVQVAPQEVEVSSYLVGQIDAKVDSRDFGDFEL"
     gene            complement(4464..6734)
                     /gene="nrdA"
                     /locus_tag="PXD81_001844"
     CDS             complement(4464..6734)
                     /gene="nrdA"
                     /locus_tag="PXD81_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018356743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HDL5537851.1"
                     /translation="MNKSLMVTKRNGTREQLNLDKIHRVITWAAEGLENVSVSQVELR
                     SHIQFYEGIRTSDIHETIIKAAADLITKDTPDYQYLAARLAIFHLRKKAYGQFEPPHL
                     YEHVKKLVALGKYDADLLSDYTPEEWETMNGFIDHWRDMTFSYAAVKQLEGKYLVQNR
                     VSGEIYESAQFLYLLVAACLFSKYPTASRLDYIRRFYDATSTFKISLPTPIMAGVRTP
                     TRQFSSCVLIECDDSLDSINATSAAIVKYVSQRAGIGINAGAIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATVYFPIWHLEAESLMVLKNNRGVEENRARHMD
                     YGVQINKMMYQRLIKGGEISLFSPSDVPGLYEAFFADQAKFEQLYLQYEQDPSIRQRK
                     VKAVELFSLLMQERASTGRIYIQNVDHCNTHSPFNPRVAPIRQSNLCLEIALPTKPLA
                     HFYDEQGEIALCTLSAFNLGTLSNLDELEELADLVVRALDALLDYQDYPVLAARNSSL
                     NRRALGVGVINYAYYLAKNQVRYSDGSANNLTHRTFEAIQYYLLKASMNLAKEQGACA
                     YFDQTTYAKGLLPIDTYKKEIDELTQEPLHYDWEQLRQDILQFGLRNSTLTALMPSET
                     SSQISNATNGIEPPRGYISVKASKDGILKQVVPEYERLKAYYELLWDIPNMDGYLHLV
                     GIMQKFVDQAISANTNYDPNRFAEAKVPMKVLLKDLLTAYKYGLKTLYYQNTRDGAED
                     VQDDLDEGCAGGACKI"
BASE COUNT         2131 a         1389 c         1296 g         1936 t
ORIGIN      
        1 aaataatatt atttttatca tcaagcaaaa catcaaaaac cccgaatctt attctattaa
       61 cggggccgta ggtttactgg ctagtgcttt agaaaatttg atccgaaacg gccaaaaata
      121 tgcaaaaaaa atgctgtcgg tctcaatgaa tgtagaagat ggtcatttat ttctcacagt
      181 tgaagatgat ggcgaggggg ttcctgaaaa cgaatatgaa aatattctta aaccttttta
      241 ccgcgtagat gaagcaaggg caagagaaac aggaggagcc gggctaggat tagcgattgt
      301 gcaaaatgta gttaaacagc accaaggtac tattcattta gataaaagta atatgggagg
      361 attaaaaatc gaactgaaac ttcccctttg gcgttcctaa ggcaaaaagc atatcgtttg
      421 taaaaaacag tcgaaaaata accgcttgta tgaaattaat tgaaatagaa aatctcaata
      481 aatactttgg cttggggcaa acccgagccc atattctaaa aaatatcaat ctcagtattg
      541 aacggggtga ttttgtcgca attataggtg cttcaggttc tggcaaatct accttaatga
      601 acattattgg ctgtttagat actgccagct caggtatttg ccgtattgat ggcaaagaga
      661 cccaaacaat gtctgcagat gaactgtctg atttacgcca acgtaaactg gggtttattt
      721 tccaacgcta taatctcctt tcagctttga atgcaaacga aaatgttgcc ctacctgcaa
      781 tttacgccgg aatggatagc tcatcacgca aaaaacgagc tgatacgtta ttaacgaagt
      841 taggattatc agataaaacc caacataagc cgaatgagtt atctgggggg caacagcaac
      901 gagtcagcat tgcacgagcc ttaatgaacg gcggtgaaat cattcttgca gacgagccta
      961 ccggtgcatt agattctaaa agtggcgaaa ctgtattgga aatcttgcga gatctacact
     1021 acgaaggtca taccattatt atggtaacac acgacccaaa cattgccgct caagccagcc
     1081 gtatcgttga aattaaagat gggcaaatca ttcgagatga acgccaacgt actgatgttc
     1141 aagaaacggc tctcaataaa acgacaacac aaaaacctcg cttttggaat caactgtttg
     1201 aatccttcaa aatggcaacc agtgcaatta tggcgcataa aatgcgggca atcctcacga
     1261 tgctgggcat tattattggc attacctctg tgatttccgt agtcgctctc ggtcgaggct
     1321 cgcaacaaca aatcctttct aatatcaaca acctaggcac aaatacgatg gatattatga
     1381 atggaaccgg ctttggcgat agaagagcca atctgactaa gaatttaacc atcagtgatg
     1441 ccttaatgtt gagtcggcaa aactatgtgg aaagcactac tccttcaagc aatgtcagtg
     1501 ccacattgat ttatggtaat accgcagtaa ccggttcagt acgaggtgtt ggagagcaat
     1561 ttattgatgt gaaaggatta aagctgattt cagggcgctt tttcagtgcc gaggaagtaa
     1621 aaaatgtagc gcaggttatt gtaattgacc ctaacactca aaaagatttg gggctaacag
     1681 agcctgttga aggtaaaacg attttagtgg ataaaaaacc gttacaaatc attggtgtgg
     1741 cgcaacagtc aaacaatatg aaccaaagta gccttacctt atgggcgcct tataccacta
     1801 ttatgcaacg catttcaggt gcaaaacaga tagattcact cacggttaaa attaaagatg
     1861 atattgaaag ccaaactgcc gaaaaaagca ttgccgaatt gttagtagct aaacacggca
     1921 aaaaagattt ctttattatg aatagtgata cgatcaaaca aaccattacc agtaccacca
     1981 atacaatgac tttgctgatc tcctctatcg cccttatttc gctgattgtc ggtggtatcg
     2041 gggtaatgaa tattatgctc gtttccgtca ccgaacgcac ccgagaaatt ggtgtccgaa
     2101 tggcgattgg cgcaaaacaa cgcaatattt tgcaacaatt tttaattgag gcggtggtta
     2161 tttgcctaat aggtggtgtg attggcattt tactggcaag cgctattatt tgggcattta
     2221 ataccttagg tacgaatttt aaaatgatgc tttcaccgga atcggtgatt attgccgtgc
     2281 tctgctctac acttatcggt gtggtatttg gctacattcc ggcaagaaac gccgcccaat
     2341 taaacccaat tactgctctg gcacaagagt aaatgcgata actatctgct acactgtagg
     2401 ggcaaaaaat tttttgcccc tacatccatt attatgccaa gtttaaggag ataaaatgac
     2461 aataaccaat actgaactag aacaaattct gaacaccaaa ctcaacagct ccgccattaa
     2521 cgactatgcc ccaaatggct tgcaagttga agggaaaaga gagatcaaaa aaattattac
     2581 cggtgtgaca gctacacttc ccctgattga aaaagcgatt gagaaaaacg ccgatgcaat
     2641 tttagtccac cacggttatt tttggaaaag tgaaaacccg tgcattcgag gaatgaaagg
     2701 taaacgcatt aaacagcttt tagtgaatga catcaatctc tttggttatc acctaccact
     2761 ggatattcac tccgaactag gtaacaacgc ccagcttgct aagcatttag gagtaaaaaa
     2821 tctacaaggt ttagaagaca gacctaattc aattccaatg tacggtgaat tagaaaatcc
     2881 gatttccgcc aatgaactga aacaaaaact tgaaaaaacg ttacaacgca ccgttatttt
     2941 atgtgatgaa tttgttcata gcccacaaaa aatgattaaa acaataggga tttgctccgg
     3001 cggaggacaa ggctatattg atttagcctt tgaaaaaggc tgtgatgcct ttattagtgg
     3061 cgaaatttcc gaacagacca ctcactctgc ccgtgaacaa ggaatctatt attttgcttg
     3121 cggtcatcac gccacggaac gagatggcgt aaaagcctta ggtgaatggt tagccgttga
     3181 atatggttta gatgttgaat ttattgatat tgataaccct gcataaaaaa tacaagcggt
     3241 tgaatttcct gaaaattttg caatttgcaa aaaatcacta aaaatcaacc gcttgtatat
     3301 catagtctaa agttcaaaat cgccaaaatc acggctatct actttagcat caatctggcc
     3361 aaccaaataa gaactgactt ccacttcttg tggtgctacc tgtacattat cggaaaccaa
     3421 ccacgcattg atccacggaa tggggttgga acgtgcctta aatggtaatg gtagtccaac
     3481 agcctgcatc cgaatattag tgatatattc aatatattgc accaaaatat cccgatttag
     3541 ccctatcatc gaaccgtctt taaacaggta ttccgcccac gctttttctt gttcagctgc
     3601 acttaaaaaa agctcgtagg cctcttggcg acattcacgt gcaatttctg ccatttcagg
     3661 atcatcattg ccataggcca taatattcaa tatatgctga gtgccggtaa gatgtaatgc
     3721 ttcatcacgt gcaataaatt tgatgatttt ggcatttcct tccataagct gccgttccgc
     3781 aaaggcaaat gagcaagcaa aagagacata aaaacgaatt gcttcaagcg cattcacact
     3841 catcatacag agataaagtt gtctttttag actacgcaaa ctgacctcac aggtttttcc
     3901 atcaacctga taactgcctt caccatacaa actatagagt tggctgtcac gaatcaaatc
     3961 atcataatag gcggaaatat ctttggcacg cttaataatt tcttcgtttg tgacaatatc
     4021 atcaaatact attgatggat cattgacaat attacggatg atatgtgtgt aagaacgact
     4081 gtgaattgtt tcgctaaatg tccaagtttc aatccaggtt tcaagctcgg ggatagacac
     4141 taatggcaaa agcgccacat tcgggctacg cccttgaatg gaatccaaca aggtttggta
     4201 ttttaaattg ctgataaaaa tgtgcttttc atgttcaggc agttgggcat agtcaatgcg
     4261 gtcttgtgac acatccactt cttccggtcg ccagaaaaat gagagttgtt tttcaatcag
     4321 tttttcaaaa atttcgtatt tttgttgatc ataacgcgcc acattcacat tttgtccaaa
     4381 aaacatgggt tcttttagtt gatcattttt atgttgtgaa aacgtagtat atgccatgtt
     4441 attctccttt atttctgttc ttattaaatt ttacaagcac caccagcaca gccttcatct
     4501 aagtcgtctt gcacatcttc tgctccatca cgggtatttt gatagtaaag ggtttttaac
     4561 ccgtatttat acgccgttaa tagatctttt aataatactt tcatcggtac tttagcttct
     4621 gcaaagcggt ttgggtcgta atttgtattc gccgaaatcg cttggtcaac aaatttctgc
     4681 ataatcccaa ccaaatgcaa atatccgtcc atattaggaa tatcccaaag cagttcatag
     4741 tatgctttta aacgttcata ctccggcaca acctgcttta aaataccgtc ttttgatgct
     4801 ttaacactaa tatagccacg cggaggttca attccattgg tggcattcga aatttgcgac
     4861 gaagtctccg atggcattaa ggcagttaaa gtggaattgc gcaagccaaa ttgcagaata
     4921 tcctgacgta gttgttccca atcataatga agcggttctt gagttaattc atcaatttct
     4981 tttttataag tatcgatagg gagtaaccct ttggcataag tggtttgatc aaaataagca
     5041 cacgcccctt gttctttggc taaattcatc gatgctttta ataaataata ctgaatcgct
     5101 tcaaaggtac ggtgagttaa attattagca ctgccatcgg aataacgcac ttggttttta
     5161 gctaaatagt aagcatagtt aatcacgcct acacctaatg cgcgacgatt taatgagctg
     5221 ttacgtgcag ctaataccgg ataatcttga taatctaata gggcatctag cgcacgaacc
     5281 actaaatcag ccaattcttc taactcatct aaattgctga gtgtgcctaa attaaatgcc
     5341 gataatgtac ataatgcgat ttccccttgt tcatcataaa aatgtgccag tggcttagtt
     5401 ggcaaagcaa tttctaagca gaggttcgat tggcgtatcg gtgcgacacg aggattaaat
     5461 gggctatgag tattacaatg atcgacattc tgaatataaa ttcgccctgt tgaagcccgc
     5521 tcttgcatca gtaatgaaaa caattctaca gctttaactt tacgttgccg aatgctcgga
     5581 tcttgctcat attgcaaata aagttgctca aatttagctt gatcagcaaa aaacgcttca
     5641 tataaccccg gcacatctga tggactaaat aaactaatct caccgccttt aattaaacgt
     5701 tgatacatca ttttattaat ctgtaccccg taatccatat ggcgagcacg gttttcttcc
     5761 acaccacggt tattttttaa taccattaag ctttctgctt ccaaatgcca aatcggaaaa
     5821 tacacggtag ccgctccacc gcgaacacca ccttgagagc aagactttac tgcagtttga
     5881 aaatgtttat aaaagggaat acagccggta tgaaatgctt ctccaccacg aatggggcta
     5941 cctaatgccc gaattgcgcc ggcattaata ccgatcccag cacgctgtga aacatattta
     6001 acgattgcag ccgaggttgc attgatggaa tctaagctat catcacactc aattaacaca
     6061 caggaactaa attgacgagt aggtgtacgc actcctgcca taatcggtgt tggcaaagaa
     6121 attttgaaag tggaagtcgc atcataaaaa cggcgaatat aatctaagcg gcttgctgtt
     6181 ggatacttag aaaataaaca ggcagctacg agtaaataca aaaattgagc cgattcataa
     6241 atctctccgc taacacgatt ctgcactaaa tattttcctt ccaactgctt tactgctgca
     6301 taggaaaacg tcatatcccg ccagtgatca ataaatccat tcattgtctc ccattcttcg
     6361 ggcgtatagt cgctcagcaa gtctgcatca tatttaccta gcgcaactaa ttttttaacg
     6421 tgctcatata aatgcggtgg ctcaaattgt ccataagctt ttttgcgtaa gtggaaaatc
     6481 gccaagcgtg cggctaaata ttgataatct ggcgtatcct tagtgattag atcagctgca
     6541 gccttgataa tagtttcatg aatatctgaa gtgcgaatac cttcataaaa ttgaatgtga
     6601 gaacggagtt ctacttgtga aacagaaaca ttctctaacc cttctgccgc ccaagtaatc
     6661 acacgatgaa ttttatctag attgagttgc tcgcgcgtgc cattacgctt tgttaccatt
     6721 aaacttttat tcataattat taacccttta ta
//
LOCUS       DANLYI010000136         6748 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.137, whole genome shotgun sequence.
ACCESSION   DANLYI010000136 DANLYI010000000
VERSION     DANLYI010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 6748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6748
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.137"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            1..1722
                     /locus_tag="PXD81_001845"
     CDS             1..1722
                     /locus_tag="PXD81_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015432991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HDL5537852.1"
                     /translation="MNSSHANLFSQPKFLKSEEAFVFSIQNENETLQLNWDIADNYYL
                     YKKEIRVTPQNIEIGDITFPAAEQYNDEFFGQVEIYRNHLGLKVPFKEIKDNASIVVE
                     YQGCTKGFCYPPERVELKLDSILSATPEELAQLEAQAVEKSENFAKTDNMAPPENVAK
                     AEQDQLAENLANNRFSIFWFFVLGVGLAFTPCVLPMLPLLSAIVIGNKQRPSTAKALL
                     LSIAYVQGMAVTYTLLGLIVAAIGLPFQIALQSPPVLIGLSIVFILLACSMFGLYEIQ
                     LPNSWQQKLNAMSQKQQGGALGSVFLMGMIAGLIASPCTSAPLSGALLYVAQSGDLVT
                     GGLALYLLALGMGLPLILITLFGNKILPKSGEWLLKVKAAFGFVMLALPVFLLSRVLP
                     SHYEPFLWSALAVAFLIWLVDAIPSNSITKKIIKVLLLLALAIAAKPWADLAWNGSTT
                     QQQTTPHLALEKIQSLADLETKLTASKGKKVMLDLYADWCVACKEFEKYTFSDQNVQN
                     KLNEMVVLQIDMTKNSAENIELMKHFNVLGLPTILFFDENGNEMSQSRVTGFLDAEQF
                     LAWLNKL"
     gene            1732..2301
                     /locus_tag="PXD81_001846"
     CDS             1732..2301
                     /locus_tag="PXD81_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005821628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HDL5537853.1"
                     /translation="MNQENHKIEDIIRIFNQCFSDEYNTRLERGGNYPIYLPEFMDED
                     GVPSERPYNVILFAHGYYSSALHEIAHWLVAGEARRKLEDFGYWYEPDGRSAERQREF
                     ESAEIKPQAIEWVLATAAGFRYFASADNLSGNPGDNSAFKQAVYDQVKLYAERGYLPK
                     RAETLRKALCAFYGTPDVIDLAQFDIERI"
     gene            2527..4305
                     /locus_tag="PXD81_001847"
     CDS             2527..4305
                     /locus_tag="PXD81_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HDL5537854.1"
                     /translation="MSTLDFHFIHRFHQQVQALSHHPALRFMQDEKWSEISWLELQKQ
                     VNNLSYALLANGLAVQEKIAIFAQNMPRWTITDIAAMQIRAVAVPIYATSTTKQLEYI
                     LNDGDIKILFVGDQEQYDQAIEIANNCSLLIKIVAMKDSIDLRDFPIAQHWQDFIKVN
                     QDEAELTKRLNDKCLEDLFTLIYTSGTTGEPKGVMLDYANLAHQLQAHNKALPHIDEN
                     DISLSFLPLSHIFERAWVTYVLYRGAVNCYLEDPQQVRQALSTVRPTLMCAVPRFYEK
                     IYTAVWDKVQQAPIHRRALFKWAIRTAEDNLFGKKSAFFHRLQYIMADKLVLGKLRAL
                     LGGRIRMMPCGGAKLDPSIGLFFQSIGINVKLGYGMTETTATISCWQEKGFNINSTGT
                     LMPDVEVKISEENEILVRGGLVMKGYYKKPEATAQAFTAEGFLKTGDAGELDSEGNLY
                     ITDRIKELMKTSNGKYIAPQYIEGKIGKDKFIEQIAVIADAKKYVSALIVPCFDSLEE
                     YAKKLNIRYQDRLELIKHYEVVRMFEQRINELQKDLASFEQIKKFTLLSQAFSTKMEE
                     ITPTLKLRRKVILERYKQQIEKMYES"
     gene            complement(4338..4682)
                     /locus_tag="PXD81_001848"
     CDS             complement(4338..4682)
                     /locus_tag="PXD81_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005600044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HDL5537855.1"
                     /translation="MIQRFEVGSRFSEMAIHNGVAYLAGQVPLDDTADAYTQTQQVLA
                     EIDKWLAKANSDKSKILMATVYLKEMADYAEMNRAWDEWVAPNNAPPRAAVEAKLANP
                     NWKVEIVVTAAV"
     gene            complement(4691..5239)
                     /locus_tag="PXD81_001849"
     CDS             complement(4691..5239)
                     /locus_tag="PXD81_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane lipoprotein lipid attachment
                     site-containing protein"
                     /protein_id="HDL5537856.1"
                     /translation="MAKLFANSTACLYLENMKKLIFLGLSALALAGCSSTISNPNQLI
                     GQWQCTIEYDDFNIRTVDNLRFSTNGTMTNQGGIYYPIQKSIFEYSLQQNGRWSVKNG
                     AIVYRIVSESVQRSHRSQIWSELQRDAELQQFEENLFSSLSDSDNNKTVELIITDFDE
                     KEMDIKQEIKGHKAYKGQCIKQ"
     gene            complement(5266..6303)
                     /locus_tag="PXD81_001850"
     CDS             complement(5266..6303)
                     /locus_tag="PXD81_001850"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HDL5537857.1"
                     /translation="MALKELDWANLGFSYIKTDYRFIAHWKDGKWNEGELTQDNTLHI
                     HEGSTALHYGQQCFEGLKAYRCKDGSINLFRPDQNAERMQRTADRLLMPRVPTELFIR
                     ACKKVVKANQDWLGPYGSGATLYLRPFLIGVGENIGVKAAPEFIFSVFCCPVGAYFKG
                     GLAPSNFITTDYDRAAPMGTGGVKVGGNYAASLLPHELAVEQSSTTRKFADAIYLDPK
                     THTKIEEVGAANFFGITKDNKFITPISESILPSITKYSLLHLAQERLGMEAIEGDVYI
                     DQLDQFAEAGACGTAAVITPVGGIQHKDKFHVFYSETEVGPVTRRLYAELTGIQFGDV
                     EAPQGWIVKVE"
     gene            complement(6445..>6748)
                     /gene="grxD"
                     /locus_tag="PXD81_001851"
     CDS             complement(6445..>6748)
                     /gene="grxD"
                     /locus_tag="PXD81_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005705415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Grx4 family monothiol glutaredoxin"
                     /protein_id="HDL5537858.1"
                     /translation="QQISENPILLYMKGSPKFPSCGFSARAVEAVINCQVPFGYVDIL
                     TNPDIRAELPKFANWPTFPQLWVEGELVGGCDIVLEMLQKGELQTLLKETAAKHAA"
BASE COUNT         2000 a         1413 c         1327 g         2008 t
ORIGIN      
        1 ataaactcat ctcacgcaaa tcttttctct caacccaaat tcctaaaatc ggaagaagcc
       61 tttgtttttt caatccaaaa tgaaaatgaa acattacagt taaattggga tattgcagac
      121 aattattatc tttataaaaa agaaatccgt gtcacgccac agaatattga gataggcgac
      181 atcacttttc ccgctgccga gcaatataac gatgaatttt tcgggcaggt ggaaatctat
      241 cgcaaccact tagggctaaa ggtaccgttt aaagaaatta aagacaacgc ctcaatcgta
      301 gtggaatatc aagggtgtac caaaggcttt tgctatccac cggaacgggt tgagcttaaa
      361 ttagacagta ttctctctgc tacacctgaa gaactggcgc aactggaagc acaagcggtc
      421 gaaaagagcg aaaattttgc aaaaaccgac aatatggcgc cccctgaaaa tgtggcaaaa
      481 gcagagcaag atcaactggc tgaaaattta gctaacaatc gtttttccat tttttggttt
      541 tttgtattag gcgttggatt agcattcact ccttgcgtac tgccgatgct accacttctc
      601 tctgcgattg taattggaaa taagcaacgc ccttctaccg ctaaagcatt gctgttaagt
      661 attgcctatg tgcaaggaat ggcagttact tacaccttac ttggcttaat tgtagcggct
      721 atcggcttgc ctttccaaat cgccttacaa agcccaccgg tgttgattgg tttatctatc
      781 gtctttattc tgcttgcctg ctcaatgttt ggcttatacg aaatccagct accaaatagc
      841 tggcaacaaa aactcaatgc aatgagccaa aaacagcaag gtggtgcgtt gggtagcgtg
      901 tttttaatgg ggatgatcgc aggcttaatt gcttcccctt gtacttctgc cccactttcc
      961 ggtgcgttgc tttatgtggc acaaagtggc gatttagtca ccggtggctt agccctctat
     1021 ctactcgcac tgggaatggg cttaccgctg attttgatta ccttattcgg caacaaaatt
     1081 ctgccaaaat ccggcgaatg gctgttaaaa gtgaaagctg cattcggttt tgttatgctt
     1141 gccttaccgg tgttcttatt aagccgtgtg ctgccaagcc actatgaacc gtttttatgg
     1201 tcggcccttg cggttgcatt tttgatctgg ttggtggatg cgattccatc taattcaatc
     1261 acgaaaaaga tcatcaaagt cttattattg ctcgctttag ccattgccgc taaaccttgg
     1321 gcagatttag cttggaacgg ctcaaccact caacagcaaa ctacaccgca cttagcgtta
     1381 gaaaaaattc aatcacttgc cgatttagag acaaaattga ccgcttccaa aggtaaaaaa
     1441 gtgatgctcg atctctatgc cgactggtgt gtcgcctgca aagagtttga aaaatacacc
     1501 tttagtgacc aaaatgttca gaacaaactg aatgaaatgg tagtgttgca aatcgatatg
     1561 acgaaaaact cggctgaaaa tatcgagctg atgaaacact ttaatgtatt aggtttgcca
     1621 acgattctgt tttttgatga aaacggcaat gaaatgagcc aatctcgtgt tactggcttt
     1681 ttggatgccg agcaattttt agcttggctg aataagttat aaagaaaaga aatgaatcaa
     1741 gaaaatcata aaattgaaga cattattcgc atttttaatc aatgcttttc tgatgaatat
     1801 aacactcgcc tagagcgggg gggtaattat ccgatttact tgcccgaatt tatggacgaa
     1861 gatggtgtgc cgagcgaacg cccttacaat gtgattttat ttgctcacgg ctattacagc
     1921 agtgccttgc acgaaattgc ccattggctc gttgctggag aagctcggcg taaacttgaa
     1981 gattttggct attggtacga gccggacgga cgctctgccg aacgccaacg tgaatttgaa
     2041 agtgcggaaa tcaaaccgca agcgattgaa tgggtgttag caactgctgc cggttttcgt
     2101 tattttgcta gtgccgacaa tctctcgggc aatcccggcg ataattcagc ctttaaacaa
     2161 gcagtgtacg accaagtcaa gctctatgca gaacgtggct accttcccaa acgagctgaa
     2221 accttgcgta aagccctctg tgctttttac ggtacacctg atgtgattga tttagctcaa
     2281 tttgatattg aacgaattta gttgcaaaat ttttaacaaa acagaccgct tgttgcatat
     2341 acaagcggtc tcatttttga aaaatattgc atttgtacta aacgataccc tacttatcta
     2401 tttctattgc cttgattact tcctatccaa aaccctttgt ttcagagtac aactgtttct
     2461 ttttttcata ttttctatta tattagcgta caactgttca ctgaaataga aagtaaaaat
     2521 aagattatga gcactttaga ttttcacttt atccaccgtt tccaccagca agttcaggct
     2581 cttagccacc acccagcttt acgttttatg caagatgaaa aatggtcaga aataagttgg
     2641 ttagaattac aaaaacaggt caataatctt tcttatgcct tgctggcaaa cggtcttgcc
     2701 gttcaagaga aaatcgctat ttttgcacaa aatatgcctc gttggacaat taccgatatt
     2761 gctgcaatgc aaattcgtgc ggtagcagtg ccgatttatg caaccagtac caccaaacaa
     2821 cttgaatata tcctgaatga cggggatata aaaatcctct ttgttggcga tcaggaacaa
     2881 tatgatcaag ccatagaaat agcgaacaac tgttcgctat taataaaaat tgtcgcgatg
     2941 aaagactcaa tagatctgcg tgatttccct atcgctcaac attggcaaga tttcattaag
     3001 gttaatcaag atgaagctga attaaccaaa cgtttaaatg ataaatgctt agaggattta
     3061 ttcactttaa tttatacctc gggaacaacc ggtgagccta aaggtgttat gttagattat
     3121 gctaatttag ctcatcaact tcaggcacat aataaagcac tccctcatat tgacgaaaat
     3181 gatatatccc tttcattttt acctttatcg catatctttg aacgtgcttg ggtcacttat
     3241 gtgctataca gaggggcagt aaattgctat ctggaagatc ctcaacaggt acgtcaagca
     3301 ctctcaacgg tacgacctac attaatgtgt gccgtaccaa ggttctatga aaaaatctat
     3361 actgcggtgt gggataaagt ccaacaagca ccgatacatc gccgagcgtt atttaaatgg
     3421 gcaatccgta ccgccgaaga caatctgttt ggtaaaaaat cagccttttt ccaccgcttg
     3481 cagtacatta tggcagataa attggtgcta ggaaaattac gagccttact aggcggtcgt
     3541 attcgtatga tgccgtgtgg aggagctaaa ttagatccgt ctatcgggct atttttccaa
     3601 agtataggca tcaatgtaaa acttggatac ggaatgaccg aaactactgc cactatttca
     3661 tgctggcagg aaaaaggctt taatatcaac tcaaccggta ccttaatgcc tgatgttgag
     3721 gtcaaaatta gtgaagaaaa tgaaatttta gtgcgtggag gcttggttat gaaaggttac
     3781 tacaaaaaac cggaagccac tgctcaagcc tttactgctg aaggttttct aaaaacggga
     3841 gatgccggtg aactggattc ggaaggcaat ctttatatta ccgatcgtat taaagaatta
     3901 atgaaaactt caaacggtaa atatattgcc ccacaataca ttgaaggaaa aataggtaaa
     3961 gataagttta ttgagcaaat tgcggtgatt gctgatgcca aaaaatatgt ttctgcgttg
     4021 atagttcctt gctttgatag cctcgaagag tatgcaaaaa agctcaatat tcgctatcaa
     4081 gacagattag aactcattaa acattatgag gttgtcagaa tgtttgaaca acgcattaac
     4141 gaattacaaa aagaccttgc aagttttgaa cagatcaaaa agtttacact actttcgcaa
     4201 gcatttagca ccaaaatgga agaaattacg cctacattaa aattaagacg aaaggtcatt
     4261 ctagaacgct ataagcaaca aatcgaaaaa atgtacgaat cataatgaaa aacagagatc
     4321 gtctttctgc ttttcattta taccgcagcg gtaaccacaa tttcgacttt ccaatttgga
     4381 ttggctaatt tagcctccac cgccgctctt ggtggagcgt tatttggggc aacccattcg
     4441 tcccacgcac ggttcatttc cgcataatct gccatctctt ttaaataaac cgtagccatt
     4501 agaattttac ttttatcgct gttggctttt gccagccatt tatcaatttc tgcaagcact
     4561 tgctgggttt gggtgtaagc atcggcggtg tcatctaacg gcacttgccc tgccaagtaa
     4621 gccacaccgt tatgtattgc catttccgaa aaacggctac ctacttcaaa acgttgaatc
     4681 atttttacct ctactgtttt atacattgcc ctttataagc tttatgccct tttatttctt
     4741 gcttgatatc catttctttt tcatcaaaat cagtgatgat tagttccact gttttattat
     4801 tatcactatc agaaagtgag ctaaataaat tctcttcaaa ttgttgtagc tccgcatcac
     4861 gttgtaattc agaccatatt tgtgaacgat ggctacgctg tacagattct gagacgatac
     4921 gataaacaat agccccgttt ttcacactcc agcgcccatt ctgttgtaat gaatactcaa
     4981 aaatcgactt ttgaataggg taatatatac caccttgatt ggtcatagtc ccatttgtgg
     5041 aaaatcgtaa attatccacc gtacgaatat taaaatcatc gtactcaata gtgcattgcc
     5101 attgcccaat cagttgatta ggattagaaa ttgttgagga acaaccagct aatgcaagtg
     5161 ctgataaacc taaaaaaatt aactttttca tattctctaa ataaagacaa gcggttgaat
     5221 ttgcaaaaag ttttgccaat tcgaccgctt gttacttgtg taaaattact ctacttttac
     5281 aatccagcct tgcggtgctt caacatcgcc aaattgaata ccggttaatt ctgcgtataa
     5341 acggcgggtg acaggcccca cttcggtttc tgaatagaag acgtggaatt tgtctttatg
     5401 ttgaatacca ccaaccggcg taatcaccgc agccgtacca caagcaccgg cttcggcaaa
     5461 ttggtctaac tggtcgatat acacatctcc ctcaatcgct tccataccta aacgctcttg
     5521 agcaaggtgt agcaatgagt atttggtaat acttggcaaa atcgactctg aaatcggggt
     5581 gatgaatttg ttgtctttgg taatcccaaa gaagttcgcc gcccccactt cttcaatttt
     5641 ggtatgggtt ttcggatcta aataaatcgc atcggcaaat ttacgggtag ttgaactttg
     5701 ctcaaccgcc aattcgtgcg gtaataaact cgcagcgtag ttaccgccta ctttcacacc
     5761 acctgttccc attggggctg cacggtcata gtcggttgta atgaagtttg agggtgctaa
     5821 gccacctttg aaatatgccc ccaccggaca gcagaatacg gaaaaaataa attcaggtgc
     5881 agctttcacc ccaatatttt cgcccacgcc gattaaaaac ggacgtaaat aaagggttgc
     5941 ccccgaaccg taaggcccta accaatcttg attggctttt accacttttt tacaggcacg
     6001 aataaacagc tcggttggca cacgtggcat taataaacga tctgcggtgc gttgcatacg
     6061 ctcggcattt tgatccgggc ggaataagtt aattgagcca tctttacagc gataggcttt
     6121 taacccctca aaacattgtt gcccatagtg cagggcagtt gagccttcgt gaatatgcag
     6181 tgtattgtct tgagttaatt cgccttcatt ccattttccg tctttccaat gtgcaatgaa
     6241 acggtaatcg gttttgatat aagaaaagcc taaatttgcc caatctaact cttttaatgc
     6301 catgttgtgt ccttttattt ttatgttacg aaataatttt gacattctaa aacaaaatag
     6361 ctcttgaggt aagaagttta agcctaaatc gcaagaaaaa attaaggctc ttaaacataa
     6421 gagccttaac atactggttt gaaattatgc tgcgtgtttt gctgcggttt cttttaataa
     6481 ggtttgaagt tcgccttttt ggagcatttc taatacgatg tcacaaccgc caactaattc
     6541 accttctacc cataattgtg ggaaagtcgg ccagtttgca aattttggca attccgcacg
     6601 aatatccgga ttggttaaaa tatccacata accgaaaggc acttggcagt taattaccgc
     6661 ttccaccgca cgagcggaga aaccacaaga tgggaatttc ggtgaacctt tcatataaag
     6721 tagaatcgga ttttcgctga tttgttgt
//
LOCUS       DANLYI010000137         6657 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.138, whole genome shotgun sequence.
ACCESSION   DANLYI010000137 DANLYI010000000
VERSION     DANLYI010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 6657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6657
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.138"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            164..433
                     /locus_tag="PXD81_001852"
     CDS             164..433
                     /locus_tag="PXD81_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005540356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL5537859.1"
                     /translation="MKIMAVCGSGLGSSFMMEMNIKKVLAKLGVEAEVSHTDLSSVVA
                     DDADLFVMAKDIAASSNVPKEKLIVVKNIVGLAEFEEKLTAYFNQ"
     gene            446..1796
                     /locus_tag="PXD81_001853"
                     /pseudo
     CDS             446..1796
                     /locus_tag="PXD81_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013745980.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
     gene            complement(1891..3039)
                     /gene="dprA"
                     /locus_tag="PXD81_001854"
     CDS             complement(1891..3039)
                     /gene="dprA"
                     /locus_tag="PXD81_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HDL5537860.1"
                     /translation="MAYHYDLLRLLQVPQMGAQRIGRLLAELNFAEFCHYDKLQLKQI
                     GWNEKQIQRWFNPDQKWIDQAMTWSELPNQQIVTLFDENYPFLLKQISTAPPLIYVKG
                     NVESLSLPQIAIVGSRDYSHYGEYWAGQFAAELARHNIIITSGLAIGIDGFAHQKIVM
                     EQGITIAVLGSGLNQVYPARHKKLAEQIVDLGGALVSEFSPNQPPVAENFPRRNRIIS
                     GLSLGTLVIEATLNSGSLITARYALEQGREVFALPNAVQNPYAQGCHKLIKEGALLVE
                     GVEDILEVVASQYQPQQIPLFDESKPTVSTSLQAVKNSEKFAKNLPSLTACQQQIYQH
                     ISLEPMAIDDLAKVTELTIEILLVELLGLELAGVIKQVSGGYVMDNGY"
     gene            3128..3640
                     /gene="def"
                     /locus_tag="PXD81_001855"
     CDS             3128..3640
                     /gene="def"
                     /locus_tag="PXD81_001855"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005621146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HDL5537861.1"
                     /translation="MAILDVVLYPDEKLATVCEPVEQVDEALNRFIDDMFETMYEHEG
                     IGLAAPQVGVLKRVITIDIEGDKTNQVVLINPEILESSGETGIEEGCLSIPGHRALVP
                     RKEKVKVKALNRQGEEIIIDADDLFAICIQHEIDHLNGVLFVDHISNLKRQRIKEKMQ
                     KLKKQIARAK"
     gene            3734..4660
                     /gene="rfaD"
                     /locus_tag="PXD81_001856"
     CDS             3734..4660
                     /gene="rfaD"
                     /locus_tag="PXD81_001856"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011609759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HDL5537862.1"
                     /translation="MIIVTGGAGMIGSNIVKALNEIGRKDILVVDNLKNGEKFINLVD
                     LDIADYCDKEDFIASIIAGDDFGEIDAVFHQGACSATTEWDGKYLMQNNYEYSKELLH
                     FCLDRQIPFFYASSAATYGGRSDNFIEKREFEGPLNAYGYSKFLFDEYVRRILPEAES
                     PVCGFKYFNVYGPREQHKGSMASVAFHLNNQMLKGENPKLFAGSEQFLRDFVYVEDVA
                     KVNLWAWQNSISGIFNLGTGKAESFQEVANAVIKYHGKGEIETIPFPDHLKSRYQTFT
                     QADLTNLRAAGYTGTFKTVAEGTAEYMAWLNR"
     gene            complement(4728..5258)
                     /gene="fabA"
                     /locus_tag="PXD81_001857"
     CDS             complement(4728..5258)
                     /gene="fabA"
                     /locus_tag="PXD81_001857"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017807287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HDL5537863.1"
                     /translation="MNSCTPIIKPSYTYEELLLSGKGELFGEKGPQLPAPPMLMMDRI
                     IEMNQEKGNFEKGYIEAEFDIKPDLWFFNCHFINDPVMPGCLGLDAMWQLVGFYLGWI
                     GGEGKGRALGVGEVKFTGQILPTAKKVTYRIHMKRVVNRKLVMGVADGEVEVDGRVIY
                     TATDLKVGLFQDTTAF"
     gene            complement(5371..>6657)
                     /locus_tag="PXD81_001858"
     CDS             complement(5371..>6657)
                     /locus_tag="PXD81_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease"
                     /protein_id="HDL5537864.1"
                     /translation="NIRSLLLDITQWDKLKQALLFGEYEPNSVNHSPYLSAKEKSTFK
                     LILVGEREDISTLSAYDDGLYQFGQYTEIKSYLSLSDNVEQWGGYVQNYAENLINKRL
                     TSTALNQFLQAYIRESESRELVSISPTLLRKHLLGIQNFYPNVTEIDEVKGYFDYLEQ
                     QSSILNQYTVGDILTNQLYIETEGEEIGQINGLSVIEFEGIPHSFGEPLRMSCNVQYG
                     DGEITDIERKVELGGNIHSKGIIIAQSCLANLLELPTQLPFSASIAFEQSYGEIDGDS
                     SSLAIFNVLVSALAKVGLPQSIAVTGAIDQFGNVLSVGGVNQKIEGFFNICHARQLTG
                     KQGVIIPAACVSHLSLSSEVVEAVEQGLFKIWTVENVFDAIAILFERDFYDDEKAPNS
                     AQTALFTLIHQNLDERESEETSGSFLSKICKKIKLD"
BASE COUNT         1953 a         1413 c         1289 g         2002 t
ORIGIN      
        1 caaaatgcca actgattatt ttttatctaa ttaaatacta aaaatgttaa cgctatcaca
       61 tttttaatat tttctgtttg atagcgttaa catttctatt ctatagtatt tctatagttc
      121 aaaattcaga caactattca ttcaccttta atggagattt actatgaaaa ttatggcagt
      181 atgtggttcc ggcttaggta gcagctttat gatggaaatg aacattaaaa aagtattggc
      241 aaaactcggt gtcgaagcgg aagtgagtca taccgacctt tcctctgttg ttgctgatga
      301 tgctgattta tttgtgatgg caaaagatat tgccgcaagc agcaatgtgc cgaaagaaaa
      361 actcatcgtg gtaaaaaaca ttgttggatt agcagaattc gaagaaaaat tgaccgctta
      421 ctttaaccaa taacggagat gttatatgga atcgctactc ttttttatat tagatattct
      481 caaagtgcct tccgttctag tgggtttaat tgctctggtc ggtttagttg cacagaaaaa
      541 atcggtatcc gatattgtta aaggaacaat caaaactatt ttaggcttct tagtgttaag
      601 cggtggtgca acagtacttt taagctccct gactccttta ggcggtatgt ttgaacacgc
      661 cttcaacgtg caaggcatta tccctaataa cgaagcgatt gtgtctatgg caatcgaaaa
      721 atatggtacg gcgactgcgt taattatggc attcggtatg gtagccaata ttttaattgc
      781 acgttttacc cgcctaaaat tcattttctt aaccggacac cataccttct atatggcgtg
      841 tatgatcggc gtaatcctaa ccgttgccgg ctttgaaggt attcagctcg tctttgtcgg
      901 ggcattaacc ttagggttaa ttatgtttcc cgactatcgc tcacttctat atgaaaaaag
      961 tgaccgctag caatgatgtt gccttaggtc atttcggcac attgggctac gtgcttgctg
     1021 gttcaatcgg gcaactggta ggaaaaggct ctaaatctac cgaagaaatg gatttaccta
     1081 aaaacctcgg cttcttacgt gatagctcca tttctatttc actcacgatg atggcgattt
     1141 actatattct cgcccttgct tcgggcagtg aatacgtttc tacattaagc ggcggacaac
     1201 attatttagt gtatgccacc attcaagcca tcacttttgc agccggtgtt tatgtcattt
     1261 tgcaaggggt tcgtttaatt ttagccgaga tcgtcccggc atttaccggc ttctccgaaa
     1321 aattagtacc tgatgccaaa ccggctctcg actgcccgat tgttttccct tacgccccca
     1381 atgcggtatt agttggattt cttgccagtt tcttaggtgg gattttaagc ttagccattc
     1441 tagggcaact taattgggtg ttaattttac cgggcgtagt gccacacttc ttctgtgggg
     1501 caactgccgg cgtatttggg aacgccaccg gcgggcgtcg tggtgcaatt atcggtgctt
     1561 ttattcacgg catattaatt accttcttgc ctgtattctt attacctgta ctgggttcat
     1621 taggctttgc taacaccact ttctctgact cagacttcgg cggtattggg attgtgttag
     1681 gctatatggc tcaatacttc gacaagaata tcattacgct cattattgtc ggcttattcg
     1741 ctctgttggt agcctataat tatttggcga aaaaaccggc aaaactgaat acctaattaa
     1801 aatcaggtgt tatttgctgc ctgcgaggaa tcttccctgc catttcttga tggtagggaa
     1861 ttttgtttag ttaaacgaca acgctcttgt tcaataccca ttatccatca catacccccc
     1921 actcacttgt ttaatcacac cggctaattc cagacccagt aattcaacca gcaatatttc
     1981 aatcgtcaac tccgtaactt tggctaaatc atcaattgcc atcggctcta agctgatatg
     2041 ctgataaatt tgctgctggc aagcggtcag acttggcaga ttttttgcga atttttcgct
     2101 gtttttgacc gcttgtaaag atgtgcttac tgttggctta gactcatcaa acaaagggat
     2161 ttgctgaggt tgatattgcg aggcgaccac ctctaaaata tcttccacac cttccacgag
     2221 caatgcccct tctttaatta gcttgtggca accttgtgcg taagggtttt gtaccgcatt
     2281 cggtaacgca aacacttccc taccttgttc aagggcataa cgagcggtaa tcagtgaacc
     2341 actattaagc gttgcttcaa taactaaagt gccgagcgat aagccactaa taattcgatt
     2401 acgacgagga aaattttctg ccaccggcgg ctgattaggc gagaactccg acaccaatgc
     2461 accacctaaa tcaacaattt gttcggctaa ttttttatgg cgagcaggat agacttgatt
     2521 taatccactc cctaacacgg caatcgttat gccttgttcc ataacgattt tttgatgggc
     2581 aaaaccgtca ataccaatgg ctaaaccgct ggtaatgata atattatggc gagctaattc
     2641 ggcagcaaat tgcccagccc aatattcacc ataatgagaa tagtctcggc tgccaacaat
     2701 cgcaatttgt ggcagagaaa ggctttccac atttcccttt acataaatta acggtggcgc
     2761 agtcgaaatt tgttttaaga gaaacggata attttcatca aagagtgtca caatttgctg
     2821 atttggcaat tctgaccaag tcatcgcctg atcaatccac ttttgatcag gattaaacca
     2881 acgttgaatt tgcttttcat tccaacctat ttgtttgagt tgaagtttgt catagtggca
     2941 aaattcagca aaattgagtt ccgccaataa tcgccctata cgttgagccc ccatttgcgg
     3001 cacttgtaat aatcgtaata aatcgtagtg atatgccata ttttcatcct caaaatttcc
     3061 ctaataataa gaaaaggttt aaaatagcca aacgaatttt ttaaactttt cgagagagat
     3121 cacaaaaatg gcaatattag atgttgtgct ttaccccgat gagaaactgg caaccgtttg
     3181 tgaaccggtg gaacaggtgg acgaagcact gaatcgtttt atcgatgata tgtttgagac
     3241 aatgtacgaa cacgaaggta ttggcttagc tgcaccgcaa gtgggcgtac ttaagcgtgt
     3301 tattactatt gatattgagg gcgacaaaac caatcaagtg gtgttaatta accctgaaat
     3361 tttagaaagc agcggtgaaa ccggcattga agagggctgt ctctccattc ccgggcatcg
     3421 tgccttagtg ccacgcaaag agaaagtgaa agtcaaagcc cttaaccgcc aaggcgaaga
     3481 aattatcatc gatgccgatg atttatttgc gatttgtatt caacacgaaa tcgaccattt
     3541 aaacggcgtg ctttttgttg accacatatc caatttaaaa cgccaacgca tcaaagaaaa
     3601 aatgcaaaaa ttgaaaaagc aaattgcacg agcgaaataa ccaggcacaa gcggttaaaa
     3661 tggcttaaaa atttgcaaat taagtgtgat gacacgataa taaccacagt tcaactttag
     3721 aaaggataaa aatatgatta tcgtcaccgg cggcgcaggt atgattggca gtaacattgt
     3781 taaagcctta aatgaaatcg gtcgcaaaga tattttagta gtagataacc tcaaaaacgg
     3841 ggaaaaattt atcaacttag tggacttaga tattgcggat tattgcgata aagaggattt
     3901 catcgcctct attatcgccg gcgatgattt tggtgagatt gatgcggttt tccatcaagg
     3961 ggcttgctcg gcaaccaccg aatgggacgg caaatactta atgcaaaata actacgaata
     4021 ttcaaaagag ttattgcatt tttgcttaga tcgccaaatt cctttcttct atgcctcaag
     4081 tgcggcaacc tatggcggac gttcggataa ttttatcgag aaacgggagt ttgaaggtcc
     4141 attaaatgct tatggctact ccaaattcct gtttgatgaa tatgtccgcc gtattttgcc
     4201 tgaggcagaa tcaccggtct gtggtttcaa atatttcaac gtatacgggc ctcgtgagca
     4261 acacaaaggt tcaatggcaa gcgtggcatt ccacttaaac aaccagatgt taaaagggga
     4321 aaatccaaaa ctatttgccg gctcagagca gttcttgcgt gattttgttt atgtggaaga
     4381 tgtggcaaaa gtgaatcttt gggcgtggca aaacagcatt tctggcattt tcaacctcgg
     4441 caccggaaaa gcggaatctt tccaagaagt tgcgaatgcg gttatcaaat accacggcaa
     4501 aggcgaaatt gaaaccatcc cattcccgga tcatttaaaa tcccgctacc aaacgttcac
     4561 tcaagcggac ttaaccaacc taagagccgc tggctacaca ggcacattta aaaccgttgc
     4621 tgaaggaaca gcggaatata tggcgtggtt gaatcgctaa tcaaacgttg ttaaaaaagc
     4681 agaaagagct acgaaatgta gctctttttg ttataaggtt ataaggatta gaatgctgtc
     4741 gtatcttgga ataagcctac ttttaaatca gtagcagtat agatcactcg cccgtccact
     4801 tcgacttcac catcagcaac ccccatcact aacttacgat tgaccacacg tttcatatgg
     4861 atacggtaag taactttttt cgctgtcggt aaaatttgtc cggtgaattt tacttcacct
     4921 acgcccaatg cacgcccttt gccttcgcca ccaatccagc cgagataaaa accaaccagt
     4981 tgccacatcg catctaagcc taagcaaccc ggcattacag gatcgttaat aaaatggcag
     5041 ttaaaaaacc ataaatccgg tttaatatca aactcagctt caatataccc tttttcaaaa
     5101 ttgccttttt cttggttcat ttcaatgatg cgatccatca ttaacatcgg tggagccggc
     5161 aattgaggcc ctttttcacc gaataattct ccttttcctg atagcaaaag ctcttcgtaa
     5221 gtatagcttg gtttgataat tggtgtacaa ctgttcatta tttttaaaat cctgtaaagt
     5281 gaaaaaagcc tgttatatca ataactgata aatcaggctg atagtaacag acttttaatt
     5341 cagtgaacag ttgtaagctg acttgtcgga tcagtctagc tttatctttt tgcaaatttt
     5401 gcttaaaaat gaaccgcttg tttcttcact ttcccgctca tctagattct ggtgaatcaa
     5461 ggtaaataat gctgtttggg ctgagttagg ggctttctca tcatcataaa aatcccgctc
     5521 aaacagaatc gctatggcat caaacacatt ttccaccgtc caaattttaa atagaccttg
     5581 ctccactgcc tcaacaactt ctgaacttaa actcaaatgg ctaacacagg ccgccggaat
     5641 aatcacacct tgtttccccg ttaattggcg agcgtgacaa atattaaaga agccttcaat
     5701 tttctgattt accccgccca cgcttagcac attcccaaat tgatcgatag cacctgtcac
     5761 cgcaatagat tgtggcaagc caactttagc taaggcactc actagtacat tgaaaatcgc
     5821 aagagaagaa ctatccccat caatttcacc ataggattgt tcaaaagcaa tcgaagctga
     5881 aaacggtaac tgggttggta gttctaacag attcgctaaa caagattgag cgataataat
     5941 acctttggag tggatatttc cccctagctc cactttacgc tcaatatctg taatttcgcc
     6001 atcaccatat tgtacattac aactcatgcg taagggctcg ccaaaactat gcggaatgcc
     6061 ttcaaactca atgaccgata agccattgat ctgcccaatt tcctctcctt cggtttcaat
     6121 ataaagttga ttagtcagaa tatcacccac cgtatattga tttaatatcg atgattgctg
     6181 ttctaaataa tcaaaataac cttttacctc gtcaatttcg gtcacatttg ggtaaaaatt
     6241 ttgaattcct aataaatgtt ttctcaataa tgtaggggaa atagacacca actctcggct
     6301 ttcactttca cgtatatagg cctgtaaaaa ttgattaagc gctgtggaag ttaatcgctt
     6361 attgatgaga ttctcagcat aattctgaac ataacctccc cactgctcca cattatccga
     6421 taatgataag taggatttta tttcggtata ttgcccaaat tggtacaacc catcatcata
     6481 agcagataaa gtagaaatat cttctctttc ccctactaaa atcaatttaa atgttgattt
     6541 ctctttagcc gataaatagg gtgagtgatt aacagaattt ggctcatatt caccaaataa
     6601 taatgcctgt tttagcttat cccattgggt aatatccaat aagagcgaac ggatatt
//
LOCUS       DANLYI010000138         6615 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.139, whole genome shotgun sequence.
ACCESSION   DANLYI010000138 DANLYI010000000
VERSION     DANLYI010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 6615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6615
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.139"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            complement(44..3073)
                     /locus_tag="PXD81_001859"
     CDS             complement(44..3073)
                     /locus_tag="PXD81_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4981 domain-containing protein"
                     /protein_id="HDL5537865.1"
                     /translation="MSLPNYYQDPSVLHLNTTLHHAYFIPFAKGQTPNQLEREQSELF
                     TLLNGDWKFNFYPSPHDLPEDFLNTKFTHQIPVPSNWQTQGFDAHQYTNINYPIPFDP
                     PYVPHQNPCGVYQREITFNPKPNKRYLLNFEGVDSCLFVYVNGEFVGYGQISHSTNEF
                     DITKQLKQGKNSLVVVVLKWCDGSYLEDQDKFRMSGIFRDVYILEREQNYLQDFFIRY
                     QLNADLSKAELKVETQFSTYTEAVHFQLFDPENRLVCEQTSGDFCQEICNLCLWNAED
                     PTLYTLCLSYQNEIIEQKIGFRQIRVENGVMLFNNAPIKFKGVNRHDSDPKTGYSISR
                     EQALVDLRLMKAHNINAIRTAHYPNAPWFSELCDQLGFYLIAESDLESHGSSAVYIES
                     PENSIFLNVPNPNKQEEIRQQAVDNFCYFARDPLFKQAILDRNIANVERDKNRTSVII
                     WSLGNESGFGENFEVAARWVKQRDPSRLTHYEGSIYQHSAHQNDLSNLDLYSEMYPST
                     ERLEEYFSDSANHKPFILCEYSHAMGNSSGDMEDYWQTFQRYSGACGGFVWEWCDHAP
                     LLPNSELSAEQKTEKYGYGGDFGETLHDGNFCMDGLVSQQRVPHSNLLEVKNVNRPVR
                     AELKAGKIWLKNQLDFSDLADYLTVHYCFSENGETTLQGQCLVNCQPNQTACLPLTLP
                     EPNGKLQLLTLDYSLNESTALLEKGHHLGFDQITLHQAMVTPALNPRPQAVIFVQDFA
                     NEWRIQQGDYRYTFDKLKGIFSRIEKNGEAVIEQPLDFNIWRAPTDNDRLIREFWKKA
                     GYDNCYTRAYHTELMQDKNGVNITAECGIVATAQSRILTLKVNYHLAPNGEMTIQVQA
                     TRPAHLPFLPRFGLRFFLNKRNNQVSYFGYGEQESYIDKYHLAKLGAYQTSARENHTD
                     YLKPQENGSHFGTHYVQANGITVTSPQPFSFNLSPYTQEELTTKSHSYDLVESPSTVL
                     CVDYKMSGIGSNSCGPNLKPQYRLNETNFEWLLKLSF"
     gene            3251..4108
                     /gene="menB"
                     /locus_tag="PXD81_001860"
     CDS             3251..4108
                     /gene="menB"
                     /locus_tag="PXD81_001860"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005723289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HDL5537866.1"
                     /translation="MLYPSEEFLYAPVEWVDHSEGYTDIRYHKSKDGIAKITINRPEV
                     RNAFRPQTVKEMIEAFSDARFDEKIGVIVLTGEGEYAFCAGGDQKVRGDYGGYKDESG
                     VHHLNVLDFQRDIRTCPKPVVAMVAGYAIGGGHVLHMLCDLTIAADNAKFGQTGPKVG
                     SFDGGWGASYMARLVGQKKAREIWFLCRQYNAQEALDMGLINTVVPYADLEKETVRWC
                     REMLQNSPIALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNEKREP
                     DFSKFRRNP"
     gene            4191..6593
                     /gene="mrcB"
                     /locus_tag="PXD81_001861"
     CDS             4191..6593
                     /gene="mrcB"
                     /locus_tag="PXD81_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1B"
                     /protein_id="HDL5537867.1"
                     /translation="MSDVELNEQSEQEPEQKPKKRNYFLSTLFKIGVIGACSMAFYGI
                     YLDGKIRSKMDGQIWELPAEVYARIESIKIEDNLTLEQTKQALLDNGYRQVSQVATPG
                     DFKIEENQLVLLRRAFPFPETPESQRVLRLTFKQDKLTYIEDLVQGKLIEEFRLDPKL
                     LALLHSDSDEERQALRLQQYPRLLIESLILTEDRRFYQHDGISPIGIARALITNYQAG
                     RTVQGGSTLTQQLVKNLFLSNERSLVRKVNEALMSVILDFRYDKNRILETYLNEIYLG
                     QNGSYQIHGFALASQFYFGRPIQEISLDQIALLVGMVKGPSLYNPWRNPQGAVERRNV
                     VLRLLLDHQVINQELYALLSSRPLNIREKGTIYREQPAFMQALTIDLKNELGEIKSAQ
                     LSGTKIFTTLDRKQQRSAEQAVITGLEDLESKNKKVSDLQSAIVVAEYRTGKVRAIVG
                     DRLTQFAGFNRAIQSKRQIGSLVKPSVYAIALAQPELFRLNTPIQNKQISIKVGSTYW
                     TPRNYDRSFSGSVMLMDALVRSLNIPTVNIGMKVGLKKVIAKQKEMGWDNAEIPPYPS
                     TLLGSYSISPYDVTRSYQVIANEGLKTPLTTIEAIISHNGEMFYQRDSGKVSEQVLPP
                     EAAVQTMYAMQQVVERGTARSLQNDFAKLKLAGKTGTTNNARDTWFVGIDGEHVTTVW
                     LGKDNNGDTNLTGSSGALYVYKQYLERALPQAYRLPKLNTMQWVGINSYGSWNCDSGR
                     QIPVWRDKGQRYCTSAGNVVATEKPSVWDALSLKKENIEAKAEEAEGKQPIPTEEVAP
                     IE"
BASE COUNT         1888 a         1373 c         1542 g         1812 t
ORIGIN      
        1 aaaggagatg ctcgttcaat taaatttgaa cgagcatttt tcgttaaaaa ctcaatttaa
       61 gcagccactc aaaattagtc tcatttaacc gatattgcgg tttcaaattc gggccgcaag
      121 agtttgagcc aatgccgctc attttgtaat cgacacacag cacggtggaa gggctttcta
      181 ccaaatcata actgtggctt ttagtggtta gctcttcctg tgtgtaaggc gaaagattaa
      241 aactaaacgg ctgtggagaa gtaacagtta tcccatttgc ttgcacataa tgcgtaccga
      301 aatggctgcc gttttcctgc ggtttgagat aatcggtatg attttctcgg gcgctggttt
      361 gataagctcc gagtttcgcc aaatggtatt tgtcgatata actctcctgc tcgccatagc
      421 caaaatagct cacttggtta ttccgtttat tcaagaaaaa ccgcaagcca aagcgaggta
      481 aaaacggcaa atgagcgggg cgggttgcct gaacctgaat cgtcatttcg ccattcgggg
      541 caagatgata attcactttc agggttaaaa tccgactttg agcggttgcc acaatgccac
      601 attcggcagt aatgtttacg ccgtttttat cttgcatcag ttcggtatga tacgccctgg
      661 tgtaacagtt atcgtagccg gcttttttcc aaaactcacg aattaagcgg tcgttatcgg
      721 ttggggctcg ccaaatattg aaatccaacg gttgctcaat tactgcctcg ccgtttttct
      781 caatccggct gaaaatgcct ttgagtttat cgaaagtgta gcgatagtcg ccctgttgaa
      841 tccgccattc atttgcaaaa tcttgcacaa aaatgaccgc ttgcgggcgt ggattaagtg
      901 ctggagtgac catcgcttga tgcagcgtga tttggtcaaa gcctaagtga tgcccttttt
      961 ccaacagagc cgtgctttca tttaaagaat aatcaagggt taaaagttgc aatttgccgt
     1021 ttggttcggg cagcgttagc ggcaaacaag cggtctgatt tggctgacaa tttaccaaac
     1081 attgtccctg cagggtggtt tcgccgtttt cgctgaaaca gtaatgcacg gtcagataat
     1141 cagctaagtc gctgaaatcc aactgatttt tcagccaaat tttgcccgct tttaactcag
     1201 ctcgcaccgg gcgattcaca tttttcactt ctaacaaatt agagtgtggc acacgctgtt
     1261 gggaaaccaa gccgtccata cagaaattgc catcgtgcaa ggtttcgccg aaatcaccgc
     1321 catagccgta tttttcggtc ttctgttcgg cacttagctc agagttgggg agcagaggag
     1381 catggtcgca ccattcccac acaaagccac cgcacgcacc gctgtagcgt tggaaggttt
     1441 gccaataatc ttccatatca ccactcgaat tgcccatcgc gtgagaatat tcacacaaaa
     1501 taaaaggttt gtggttggca gagtcggaaa aatattcctc taaccgctcg gtgcttgggt
     1561 acatctcgct gtataaatcg aggtttgaaa gatcgttttg atgtgccgaa tgttggtaaa
     1621 tcgaaccttc gtagtgagtt aaacggcttg gatcccgttg ttttacccaa cgggctgcga
     1681 cttcaaaatt ttcaccaaag ccggattcgt taccaagtga ccaaataatg accgaagtgc
     1741 ggtttttgtc tcgctccaca ttggcaatgt tgcgatctaa aatcgcctgt ttgaacagcg
     1801 gatctcttgc aaaataacag aaattatcga ccgcttgttg gcgaatttct tcctgtttgt
     1861 tcgggtttgg cacattcagg aaaatgctgt tttccggact ttcaatatac actgcactgg
     1921 agccgtgaga ttcaagatca ctttcggcaa tcagataaaa accgagctga tcgcacaatt
     1981 cactaaacca cggagcgttc gggtaatggg cggtgcgaat agcgttgatg ttatgggctt
     2041 tcattaagcg taaatccacc aacgcttgct cacggctaat gctgtagccg gttttcgggt
     2101 cggaatcgtg gcggtttacc cctttaaatt taatcggggc gttgttgaat agcatcacgc
     2161 cattttccac acgaatctga cgaaagccga ttttttgctc aataatttcg ttttggtagc
     2221 tcaagcatag cgtgtagagt gtcggatctt ccgcattcca caaacacaaa ttgcaaattt
     2281 cttggcaaaa atcaccgctt gtttgctcgc agactaagcg gttttcaggg tcgaaaagtt
     2341 gaaaatgcac agcttcggtg taggtggaaa attgtgtttc caccttgagt tctgcctttg
     2401 aaagatcagc gtttaactgg tagcggataa agaaatcttg caggtagttt tgctcacgtt
     2461 ccaaaatata aacatcacgg aaaatgcctg acattcggaa tttgtcttga tcttccaaat
     2521 aactgccgtc acaccatttt agcaccacca cgacgaggct gtttttgccc tgtttaagct
     2581 gtttggtgat gtcaaattca ttggtgctgt ggctgatttg cccgtagccg acaaattcgc
     2641 cgtttacata aacaaacagg cacgaatcta cgccctcaaa atttagcaaa tagcgtttgt
     2701 taggcttggg gttaaacgtg atttcacgct gatacacccc gcaagggttt tggtgtggca
     2761 cataaggcgg atcaaacgga atcgggtagt tgatgttagt gtattgatgg gcatcaaagc
     2821 cttgagtttg ccagttagac ggcacgggga tttggtgggt aaatttggtg ttgaggaaat
     2881 cctccggcag atcgtgcgga cttggataga aattaaactt ccaatcgcca tttagcaaag
     2941 taaataactc agattgttcc cgttccaact gattcggcgt ttgcccttta gcaaaaggaa
     3001 taaaataagc gtggtgtagc gtggtattaa ggtgcaacac actcgggtct tggtagtaat
     3061 tcggtaacga cataaaagcc ctatgatcaa aaagatataa atacgaatac actttaattc
     3121 tcttataaaa tagggcaatc aactatgtga aaaattgtga ggcggatcac aaaataattt
     3181 cctcaccttt ttccttcgat tccgttataa tcacaagaat tttttatgtc atttttcaag
     3241 gaatttacca atgttatatc caagcgaaga atttttatat gcaccggttg agtgggttga
     3301 tcacagcgaa ggttacaccg atattcgtta ccataaatca aaagacggta tcgctaaaat
     3361 caccattaat cgtccggaag tgcgtaatgc tttccgtccg caaacggtaa aagagatgat
     3421 tgaagccttt tctgatgccc gttttgatga aaaaatcggc gtcatcgtat taaccggtga
     3481 aggcgaatat gcgttctgtg ccggcggcga ccaaaaagtg cgtggcgatt acggcggcta
     3541 caaagatgaa agcggtgtgc atcacttaaa tgtgttggat ttccaacgtg atattcgtac
     3601 ctgcccgaaa ccggtggtgg caatggttgc cggctatgcg attggtggtg gacacgtttt
     3661 acatatgtta tgtgatttaa ccattgccgc tgacaatgca aaattcggtc aaaccggccc
     3721 gaaagtgggg tcatttgacg gcggttgggg tgcaagctat atggcacgtt tagtcggtca
     3781 gaaaaaagcg cgtgaaattt ggttcttatg ccgtcaatat aatgcacaag aagcgttaga
     3841 tatgggctta atcaataccg ttgtgcctta tgctgattta gaaaaagaga ctgtgcgttg
     3901 gtgtcgcgaa atgctacaaa acagcccgat tgccttacgt tgcttaaaag cagcattgaa
     3961 tgcagattgt gacggtcagg ctggcctgca agagcttgct ggtaatgcaa caatgttgtt
     4021 ctatatgaca gaagaagggc aagaaggacg caatgcgttc aacgagaaac gtgagccgga
     4081 cttcagcaaa ttccgccgca atccataatt gacggcaaat acaagcggtt cgattttgca
     4141 aaaaatttgc aaaaatcgac cgcttacctt ttctaattta agttaatttt atgtcagatg
     4201 ttgagttaaa cgagcagagc gagcaagagc ctgagcaaaa accgaaaaag cgaaactatt
     4261 tcctttcaac cttattcaaa atcggggtga taggagcttg cagtatggct ttttacggca
     4321 tttatttaga cggtaaaatc cgtagcaaaa tggatgggca gatatgggaa ttaccagctg
     4381 aggtgtatgc tcgtattgaa agcattaaaa ttgaagacaa cttaacgctt gagcaaacca
     4441 aacaagcgtt gctggataac ggctatcgtc aagtttctca agttgcgaca ccaggcgatt
     4501 tcaaaattga agaaaatcag ctggtgttgc tacgccgagc tttcccgttt ccagaaacac
     4561 cggaatcgca acgagtgctt cgtttaactt tcaaacaaga taaattaact tatattgaag
     4621 atttggtgca gggtaagttg atcgaggagt tccgcttgga tccgaaatta ctggcgttac
     4681 tgcattccga tagcgatgaa gaacgtcaag cattacgctt acaacaatac cctcgcttgt
     4741 taattgagtc actgatttta accgaagatc gccgctttta tcagcacgat gggattagcc
     4801 caatcggtat tgcccgtgct ttaatcacta actaccaagc tggcagaaca gtgcagggtg
     4861 gcagtacctt aacgcaacag ttagtgaaaa acttgttctt aagtaatgaa agatcgttgg
     4921 tgcgtaaggt aaatgaagcg ttaatgtcgg tgattttaga cttccgttac gataaaaacc
     4981 gcattttaga aacctatctc aacgaaattt atttagggca gaacggtagc taccaaattc
     5041 acggttttgc attggcaagc caattctatt ttggtcgtcc gatccaagag attagcttag
     5101 accaaattgc gttattggtg ggaatggtaa aaggtccatc actgtataac ccgtggcgaa
     5161 atccgcaagg tgcggtagag cgacgtaatg tggtgttgcg attattgctg gatcatcaag
     5221 tgatcaatca agaactttac gctctgttaa gctcccgccc gcttaatatt cgtgaaaaag
     5281 gcacgattta ccgtgagcaa cctgccttta tgcaggcatt aaccattgat ttgaaaaatg
     5341 agttaggcga aattaagtca gctcaacttt ccggtactaa aatttttacg acccttgacc
     5401 gtaaacagca acgttcagcc gaacaagcag tgattaccgg tttggaagat ctggaaagta
     5461 aaaataaaaa agtcagcgat ttgcaatcgg ctattgtcgt ggcggaatac cgcacaggta
     5521 aagtgagggc gatagtagga gaccgcttaa cccaatttgc cggctttaac cgtgcgattc
     5581 agtcaaaacg ccaaatcggt tctttagtca aaccatcggt ttatgcgatt gccttggcac
     5641 agcctgaatt attccgttta aatacaccga tccaaaataa acaaatttca attaaggttg
     5701 gttcaaccta ctggacaccc cgtaactacg accgcagttt tagtggctcg gtgatgttaa
     5761 tggacgcctt agtccgctct ctgaatattc caacggtgaa tattggtatg aaagttggct
     5821 taaaaaaagt cattgccaaa caaaaagaga tgggctggga taatgcggaa attccgcctt
     5881 acccatcaac cttgctaggt tcatattcga tctcgcctta cgatgtaacc cgctcttatc
     5941 aagtaattgc caacgaaggg ctgaaaacac cattaaccac cattgaggcg attatttcgc
     6001 acaatggcga aatgttctat cagcgagata gcggcaaagt gagcgagcag gtgcttccac
     6061 cggaagctgc ggttcaaaca atgtatgcga tgcaacaggt tgtggaacga ggtacggctc
     6121 gtagcttaca aaacgatttt gccaaactga aattagccgg caaaaccggt acaaccaata
     6181 acgctcgaga tacttggttt gtgggaattg atggcgaaca cgtcacaacc gtctggctgg
     6241 gtaaagataa caatggggat accaacctca ccggttcaag tggtgcattg tatgtgtata
     6301 agcaatatct tgagagagcc ttgcctcagg cgtatagatt accgaaactt aatacgatgc
     6361 aatgggtggg gattaatagc tatggtagct ggaattgcga tagtggcaga caaattccag
     6421 tttggcgaga taaagggcaa cgttattgta ccagtgcagg taatgtagtg gctacagaaa
     6481 aaccaagtgt ttgggatgca ctttcgctga aaaaagaaaa tatcgaggct aaagcagaag
     6541 aagctgaagg gaagcaacca atccctactg aagaagttgc accaattgaa taaaaataaa
     6601 tttgatagcc attat
//
LOCUS       DANLYI010000139         6612 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.140, whole genome shotgun sequence.
ACCESSION   DANLYI010000139 DANLYI010000000
VERSION     DANLYI010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 6612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6612
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.140"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            29..2653
                     /gene="aceE"
                     /locus_tag="PXD81_001862"
     CDS             29..2653
                     /gene="aceE"
                     /locus_tag="PXD81_001862"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005550031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HDL5537868.1"
                     /translation="METQDWLASLDSLIREEGLERAQFIIEQVISQARAGGVALPTGV
                     TTDYVNTIPVSEQPAYPGDHKIERRIRSAVRWNAIAAVLRSQKKDLDLGGHISTFQSA
                     ATMYEVCFNHFFKAPTEKDGGDLVFFQGHAAPGMYGRAFLEGRITAEQMDNFRQEAFT
                     DGLSSYPHPKLMPEFWQFSTVSMGLGPVNAIYQARFLKYLENRGLKDTSAQKVYAFLG
                     DGEMDEIESKGALSFAGREKLNNLIFTISCNLQRLDGPVNGNGKIVQELEALFTGCGW
                     EVIKVLWGSGWDKLLEKDTSGKLKQLMMEVLDGDYLTFKSKNGAYVREHFFGRYPETA
                     ALVADMTDDEIWALRRGAHDSEKLYAAYAKAQKSDKPVVILAHQVKGYRIPEAESKNT
                     AHQSKKMSLDSLKGFRDYFELPLTDEQVESLEYVRFPEGSEEYNYLHGQRKALNGYVP
                     VRKPKFTVDFKVPELSEFQALLDEQPRGISTTMAFSRVLNTLLKDKNIGQQIVPIIAD
                     EARTFGMEGLFRQIGIYNPHGQNYVPSDRDLVAYYREAKDGQVLQEGINELGATASWV
                     AAATSYSVSNLPMIPFFIYYSMFGFQRVGDMMWLAGDQLARGFMIGGTSGRTTLNGEG
                     LQHEDGHSHIQAGVIPNCVTYDPAFAFEVAVIVQDGIRRMYGENQEDIFYYITTLNEI
                     TEQPAMPAGAEEGIRKGLYKFETVEGKGKGHVQLLSSGAIMRHVRAAAQILANDYGIT
                     ADVFSAPSFNELGRDGADVARWNLLHPTETPRVPYVAQVLQDLPTVASTDYMKMYAEQ
                     IRAYVPSKHYHVLGTDGFGRSDSRANLREHFEVDERYVVVAALAQLAKEGTVENKVVA
                     EAITKFGLNVDRINPLYA"
     gene            2857..4767
                     /gene="aceF"
                     /locus_tag="PXD81_001863"
     CDS             2857..4767
                     /gene="aceF"
                     /locus_tag="PXD81_001863"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005618949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HDL5537869.1"
                     /translation="MSKQINVPDIGSDEVSVTEVMVKVGDTISVDQSIINVEGDKASM
                     EVPAPEAGVVKEVLVKVGDKVSTGSPMLILESADAAPAQEAPKAEAAPAAPVATTSAV
                     VEVNVPDIGGDEVNVTEIMVKVGDSVEVDQSIINVEGDKASMEVPAPVAGVVKEILIN
                     VGDKVSTGKLIMKFETASAATAQSTPVAEAAPAQASASAIKDVNVPDIGGDEVNVTEI
                     MVKVGDSVSEEQSLITVEGDKASMEVPAPFAGVVKEILVKSGDKVSTGSLIMKFEVAG
                     AAPAPAAAQAASAPAPAPQAAAPAKADAAPAGQNQSGLSQEQVVASAGYAHATPVIRR
                     LAREFGVNLDKVKGTGRKGRIVKEDIQAYVKTAVQVFEKQGGTAAAATGAANGAGLGL
                     LPWPKVDFSKFGEVEEVELSRINKISGANLHRNWVMIPHVTHFDRTDITELENFRKEQ
                     NKEAEKRKLDVKITPVVFIMKAVASALEAFPRFNSSISEDAQRLTLKKYINIGVAVDT
                     PNGLVVPVFKNVNKKGIIELSRELMEVSKKAREGKLTASDMQGGCFTISSLGGIGTTH
                     FTPIVNAPEVAILGVSKSEMQPIWNGKEFEPRLMLPLSLSFDHRVIDGADGARFLSYI
                     NSVLADIRRLVM"
     gene            4967..6391
                     /gene="lpdA"
                     /locus_tag="PXD81_001864"
     CDS             4967..6391
                     /gene="lpdA"
                     /locus_tag="PXD81_001864"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011272714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HDL5537870.1"
                     /translation="MSKEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYSTLGGI
                     CLNVGCIPSKALLHVAKVIEEAKNAVHNGVTFGEPTIDLDQVRAGKEAVVSKLTGGLA
                     GMAKARKVTVVEGLAAFTGPNTLVARDRDGNPTTITFDNAIIAAGSRPIQLPFIPHED
                     PRVWDSTDALKLKEVPKKLLIMGGGIIGLEMGTVYNALGSEVEVVEMFDQVIPAADKD
                     VVAIYTKQIEKKFKLMLETKVTAVEAKDDGIYVSMEGKACNDTKRYDAVLVAIGRVPN
                     GKLIDAGKAGVEVDERGFIRVDKQMRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGMKHYFDPKVIPSIAYTEPEVAWVGKTEKECKQEGLNYEVAKFPWAASGRAIASEC
                     SEGMTKLIFDKDTHRVLGGAIVGSNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNAKAKKR"
     gene            6392..6612
                     /locus_tag="PXD81_001865"
                     /pseudo
     CDS             6392..6612
                     /locus_tag="PXD81_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248352.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
BASE COUNT         2026 a         1241 c         1531 g         1814 t
ORIGIN      
        1 agagaactta agaaatgacg tagatccaat cgaaactcag gattggttag catcactcga
       61 ttcattaatt cgtgaagaag gtttagagcg cgctcaattt atcattgaac aagttattag
      121 ccaagctcgt gcaggtggcg tggcattacc aacaggcgta acaacagatt atgtcaatac
      181 tatccctgtt tccgagcagc cagcttatcc gggtgatcat aaaatcgaac gtcgtattcg
      241 ttctgcggta cgttggaatg cgattgcggc agtacttcgt agccagaaga aagatcttga
      301 cttaggtggt catatttcaa ctttccaatc tgcggcaact atgtatgaag tgtgttttaa
      361 ccactttttc aaagcaccaa ccgaaaaaga tggtggtgat ttagtgttct tccaaggcca
      421 cgcagcaccg ggtatgtatg gtcgtgcgtt cttagaaggt cgtattaccg cagaacaaat
      481 ggataacttc cgtcaagaag catttacaga cggtttatct tcttacccac accctaaatt
      541 aatgcctgaa ttttggcaat tctctaccgt atctatgggt ttaggaccgg taaatgctat
      601 ttaccaagct cgtttcttaa aatatttaga aaaccgtggt ctaaaagata cttcggctca
      661 aaaagtatat gcattcttag gcgatggcga aatggatgaa atcgaatcta aaggtgcatt
      721 atcattcgcc ggtcgtgaaa aattaaataa cttaatcttc actattagct gcaacttaca
      781 acgtttagac ggtccggtta acggtaacgg caaaatcgtg caagaattag aagcactctt
      841 taccggttgt ggctgggaag tgattaaagt gttatggggt agtggttggg ataaactgct
      901 tgaaaaagat acttcaggca aacttaaaca gttaatgatg gaagtgcttg atggtgacta
      961 tttaaccttc aaatccaaaa atggtgctta cgttcgtgaa cacttcttcg gtcgttaccc
     1021 tgaaactgca gcattagttg ctgatatgac agacgatgaa atttgggcat tacgccgtgg
     1081 tgcacacgat tctgaaaaac tttatgcagc ctatgcaaaa gcacaaaaat cggacaaacc
     1141 ggttgtaatt ttagctcacc aagtgaaagg ctaccgtatt cctgaagcgg aaagtaaaaa
     1201 tactgctcac caatcgaaaa aaatgtcatt agacagctta aaaggcttcc gtgactactt
     1261 cgagctacca ttaaccgatg agcaagtaga aagcctagag tatgtaagat tccctgaagg
     1321 ttctgaagag tacaactact tacacggaca acgtaaagcc ttaaacggtt atgtgcctgt
     1381 acgtaaaccg aaatttacgg ttgatttcaa agtgcctgaa ttaagcgaat tccaagcatt
     1441 attggatgag caaccacgtg gtatttcaac cacaatggcg ttctctcgtg tattgaatac
     1501 tttattgaaa gataaaaata tcggtcagca aatcgtacct attattgcgg acgaagcccg
     1561 tactttcggt atggaaggtt tattccgtca aatcggtatt tataacccac atggtcaaaa
     1621 ctatgtaccg tcagaccgtg atttagtagc ttactaccgt gaagcaaaag atggccaagt
     1681 gttacaagaa ggtatcaatg agttaggtgc aacagcctct tgggtggcag cagcgacttc
     1741 ttattctgtt agcaacttac cgatgatccc attcttcatc tactactcaa tgtttggttt
     1801 ccaacgtgta ggcgatatga tgtggttagc cggtgaccaa ttagctcgtg gctttatgat
     1861 tggtggtact tcaggtcgta caacattaaa cggtgaaggt ttacaacacg aagatggtca
     1921 tagccatatt caagcaggtg taatcccgaa ctgcgtcact tatgaccctg catttgcctt
     1981 tgaggttgcg gttattgttc aagacggtat tcgtcgtatg tatggtgaaa accaagaaga
     2041 tatcttctac tacattacta ccttaaatga aattaccgaa cagccggcaa tgccggcagg
     2101 tgcggaagaa ggtatccgta aaggtttata taaatttgaa accgttgagg gtaaaggtaa
     2161 aggtcacgta caattattaa gctcaggtgc aattatgcgt cacgtgcgtg cagctgcaca
     2221 aattctcgcg aatgactacg gcattacagc agatgtattc tcagcaccat cattcaatga
     2281 attaggtcgt gatggagcag atgtggctcg ttggaactta ttacacccaa ctgaaacacc
     2341 acgtgtaccg tacgttgctc aagtgttaca agatttaccg actgttgctt caaccgacta
     2401 tatgaaaatg tacgcagagc aaatccgtgc ttatgtacca agtaaacact atcacgtgtt
     2461 aggtacagac ggtttcggtc gttcagacag ccgtgcaaac ttacgtgaac acttcgaagt
     2521 tgatgagcgt tatgtagtag ttgcagccct cgctcaatta gcgaaagaag gtacagttga
     2581 aaacaaagtg gttgcggaag ctattactaa attcggctta aatgtagatc gtattaaccc
     2641 attatatgcg taatttctaa tactctctcc cttgatggga gagaggcagc taaaggtttc
     2701 gtttagaaac ttttagcaga gagggtatta gttttatttg caaaaatgtt gagaaaatca
     2761 accgcttgca ttcccttctc cctgattttt cttcttacaa agaaaaatct gtccctccca
     2821 caatgggaga gggggtaact caagaaggat ttcaaaatgt caaaacaaat taatgtgccg
     2881 gacatcggtt cggatgaagt cagtgtgact gaagtgatgg taaaagttgg tgatactatt
     2941 tcagtagatc aatcaattat taacgtagaa ggcgataaag cctctatgga agtgccggct
     3001 cctgaagcag gtgtggtaaa agaagtttta gtgaaagtgg gtgataaagt ttcaaccggt
     3061 tcaccaatgt taattttaga gtcagcagat gctgctcctg ctcaagaagc accaaaagca
     3121 gaagcagctc ctgctgctcc agtagcaaca acttcagccg ttgttgaagt aaatgtacct
     3181 gatatcggtg gcgatgaagt taacgtaacc gaaattatgg ttaaagtagg tgatagcgtt
     3241 gaagtggatc aatctatcat caacgttgaa ggtgataaag cctcaatgga agtgcctgca
     3301 ccggttgccg gcgtagtaaa agaaatctta atcaatgtgg gtgacaaagt ttcaactggt
     3361 aaattgatta tgaaatttga aacggcttcc gcagctacag cacaatctac accggttgcg
     3421 gaagctgctc cagctcaagc ttcggcatca gcgattaaag atgtaaatgt accggacatc
     3481 ggtggtgatg aagtaaacgt aactgaaatt atggttaaag tgggtgattc tgtttcagaa
     3541 gaacaatctt taatcacggt tgaaggtgat aaagcttcaa tggaagttcc ggctccattt
     3601 gccggtgtgg tgaaagaaat tttagtgaaa tcaggtgata aagtttcaac cggttcatta
     3661 attatgaaat ttgaagtagc gggtgcggca ccggctccgg cggctgctca agcggcttca
     3721 gctcctgctc ctgctccaca agcagcagct ccggcgaaag ctgacgcagc tccggcaggt
     3781 caaaaccaat caggtttaag ccaagaacaa gtggtggctt cagctggcta tgcacacgca
     3841 acaccggtaa ttcgccgttt agctcgtgag tttggtgtaa acctagacaa agtaaaaggt
     3901 acaggtcgta aaggccgtat cgttaaagaa gatattcaag catacgttaa aacggctgtt
     3961 caagtatttg aaaaacaagg cggaacagca gcggcagcaa caggtgcggc aaacggtgca
     4021 ggcttaggtt tattaccgtg gccgaaagta gatttcagca agtttggtga agttgaagaa
     4081 gttgaattaa gccgtatcaa taagatttcc ggtgcgaact tacaccgtaa ctgggtaatg
     4141 attcctcacg ttacacattt cgatcgcaca gacattaccg agttagaaaa cttccgtaaa
     4201 gagcaaaata aagaagcgga aaaacgtaaa ttagacgtga aaattacccc tgtggtcttc
     4261 attatgaaag cagtggcaag tgcattagaa gccttccctc gtttcaacag ctcaatttct
     4321 gaagatgcac aacgtttaac cttgaaaaaa tacatcaata tcggggttgc ggttgatacg
     4381 ccaaacggtt tagtggtgcc ggtgttcaaa aatgtgaaca aaaaaggcat tatcgaactt
     4441 tctcgcgaat taatggaagt ctcgaaaaaa gcccgtgaag gtaaattgac agcatctgat
     4501 atgcaaggtg gctgtttcac aatttcaagt ttaggcggta ttggtacaac gcactttacg
     4561 ccaatcgtga atgcaccgga agtggctatt ttaggtgtgt ctaaatctga aatgcaaccg
     4621 atttggaatg gcaaagagtt tgaaccacgc ttaatgcttc cgctttcatt atcattcgac
     4681 caccgtgtga tcgatggtgc tgatggtgcg agattcttaa gctatattaa tagcgtgttg
     4741 gcagatattc gtcgtttagt gatgtaattc ggtaaagctc ctcctaaaaa agtccctctt
     4801 tagaaaagag gggtaggggg agatttgata gacgtaattt acgaagagaa taaattattt
     4861 ggtagaaatt tgtgtttgat tgattgctct ctcacttcgc tttatattct gccaaatctc
     4921 ccttacccct ctttgctaaa gggggggaat aaaaagagga aaaactatga gcaaagaaat
     4981 taaaacccaa gttgtcgtac ttggtgcagg tccagcaggt tactcagcgg cattccgttg
     5041 tgccgactta ggcttagaaa cagtaattgt tgagcgttat tcaaccttag gtggtatttg
     5101 cttaaacgta ggttgtatcc cttctaaagc attattacac gttgcaaaag taattgaaga
     5161 agctaaaaat gcagtacaca acggtgtgac ctttggtgag ccaacgattg atttagatca
     5221 ggtacgtgct ggtaaagaag ccgttgtttc aaaattaacc ggtggcttag caggaatggc
     5281 taaggctcgt aaagtgacag tagtagaagg cttggctgca tttactggtc cgaatacgtt
     5341 agtcgctcgt gatcgtgacg gcaatcctac aactatcacg ttcgataatg cgattatcgc
     5401 agcaggttct cgtccgattc aactaccatt tattccacac gaagacccac gagtttggga
     5461 ttcaaccgat gctcttaaat taaaagaagt accgaaaaaa ctccttatta tgggtggtgg
     5521 tattatcggt ttagaaatgg gaacggtata taacgcgctt ggttcagaag tggaagtggt
     5581 tgaaatgttc gaccaagtaa ttcctgctgc agataaagat gttgtagcga tttacaccaa
     5641 acaaatcgag aaaaaattca agctaatgct tgaaaccaaa gtaaccgcag tagaagctaa
     5701 agatgacggt atctatgtat caatggaagg taaggcttgc aacgatacta aacgttatga
     5761 tgcggtatta gtcgctatcg gacgtgtgcc aaacggtaaa ttaattgatg ccggtaaagc
     5821 tggagtggaa gtagatgagc gtggctttat tcgtgtagat aaacaaatgc gtaccaatgt
     5881 gccacacatc tttgctattg gtgatattgt cggccagccg atgttagctc acaaaggtgt
     5941 acacgaggga cacgtggcag cagaagtgat tgcaggaatg aaacattact tcgatccgaa
     6001 agtcattccg tccatcgctt atactgaacc agaagtggct tgggttggta aaactgagaa
     6061 agagtgtaaa caagaaggct tgaactacga agtggcgaaa ttcccttggg ctgcttcagg
     6121 tcgtgcgatt gcttctgaat gctcagaagg tatgaccaag ttaatttttg ataaagatac
     6181 tcaccgtgta ttaggtggtg cgattgttgg tagcaatggt ggcgaattat taggcgaaat
     6241 cggtcttgca atcgaaatgg gctgtgatgc tgaagatatc gcattaacca ttcatgctca
     6301 cccaacgtta cacgaatcgg taggattagc ggcagaagta tttgaaggtt caattacaga
     6361 cttaccaaat gcgaaagcca aaaagcgtta atcagattat ttaatctcta aattaggatt
     6421 aaaacaatct tttataaata acagaagata tttaggaaat aaatattccc tttctgattt
     6481 tataagaaag acagttgacg aaaattgcaa gggtgtaaat attatcattg acatatttag
     6541 tggaactgga gcagtctcta atacttttaa ggataaaatg cttattacaa atgacttgtt
     6601 gtatagcaat ta
//
LOCUS       DANLYI010000140         6556 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.141, whole genome shotgun sequence.
ACCESSION   DANLYI010000140 DANLYI010000000
VERSION     DANLYI010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 6556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6556
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.141"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            25..999
                     /gene="znuA"
                     /locus_tag="PXD81_001866"
     CDS             25..999
                     /gene="znuA"
                     /locus_tag="PXD81_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HDL5537871.1"
                     /translation="MFKRTLIGLAVLGASVAQADVLTSIKPLGFIASAITEGVTETKV
                     LLPVTASPHDYSLKPSDVTQLQSAQLVVWVGDGLEGFLEKSIEKLPREKVLTLEDVDA
                     IDDIVDKASKKEDKDDDHDHKHEHKHEHKHEHKHEGHNHEGHNHDHHGHSHDEDWHIW
                     LSSEASELAAEQIAARLSEQMPEHKAKIAENLAKFKANLTAKKAEIAKQLEPVKAKGY
                     YTFHDAYGYFEREYGLNSLGSFTINPTVAPGAKTLTAIKKNIAKNKAQCLFAEPQFTP
                     KVIETLAKETKVNVGQLDPLGAKVQLSATAYPEFLQVLADEFSQCLTK"
     gene            complement(1051..1371)
                     /locus_tag="PXD81_001867"
     CDS             complement(1051..1371)
                     /locus_tag="PXD81_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005621693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhnA domain-containing protein"
                     /protein_id="HDL5537872.1"
                     /translation="MIYPTCPECKSENTYHDSIQFVCPDCAYEWTGEETDQDDDVLIV
                     KDSNGNLLSDGDDVLLIKDLKLKGSSEVLKKGTKFKGIRLAQGDHNVDCGKIMLKSEF
                     LKKA"
     gene            1512..3875
                     /gene="rnr"
                     /locus_tag="PXD81_001868"
     CDS             1512..3875
                     /gene="rnr"
                     /locus_tag="PXD81_001868"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HDL5537873.1"
                     /translation="MIVDPNYQQELEKYENPVPSREFILNTIREHNAPMSRDELLSAF
                     HIDDEERMEGIRRRLRAMENDGELVFTKGKRYALPEKMDLIKGTVIGHRDGYGFLQVE
                     GHEKGATKSEDWFIPNAQMVKVMHGDFVLAQPNGTDRRGRKEVRIVRVLEARKKQIVG
                     RFFLESGIGFVVPDDSRVNQDILIPDEHRMGARMGQVVVVELQERKASFSRPVGIITE
                     ILGDNLAPGMEIEIALRNHDIPHIWPEGVEKQIRQFSSEEVPEEAKQGRVDLRDLPLI
                     TIDGEDARDFDDAVFAKKEGNGWRLWVAIADVSYYVRPKTALDLEALNRGNSVYFPNR
                     VVPMLPEVLSNGLCSLNPQVDRLCLVAEMSVSSKGELQDYQFYEAVMNSHARLTYTKV
                     WKMLEGDEALRERYAPLVPHIEELYAMFQTLMKARHKRGAIEFETIENQFIFNPQGRI
                     ERIEPLIRNDAHKLIEECMILANIAAARFVEKANEPALYRIHDKPSEEKLLSFKSFVR
                     ECGLTWDVGLDPTPKDYGVLLEQIAERADKELIQTMLLRSLKQAVYAADNIGHFGLAL
                     TEYAHFTSPIRRYPDLLLHRAIKYLIEKGKGNTRYYTDGGGYHYKLDDMDEFGDKCSA
                     TERRADEATREVADWLKCEFMQDHLGEEFDGVISSVTGFGLFVKLNELLIDGLVHIST
                     LDNDYYHYDADRQRLIGGSGIIYRLGDAVRVKVINVNLDEKKIDFELLTGNKKTGKTA
                     KKKAKATEVPVFKEPKTVKKAKKVAEKKPAKSAKKRTKTTASKSRKK"
     gene            3879..4145
                     /locus_tag="PXD81_001869"
     CDS             3879..4145
                     /locus_tag="PXD81_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5537874.1"
                     /translation="MDYKKQILFSLLFALVIAIISYFLFTYIPFDCSDLSKLNDSQRL
                     ECRTAEFLGVYYWLPHTTTLFFGSVLLYWAVYGMISLVQAVLKR"
     gene            4368..4826
                     /gene="mraZ"
                     /locus_tag="PXD81_001870"
     CDS             4368..4826
                     /gene="mraZ"
                     /locus_tag="PXD81_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005616487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HDL5537875.1"
                     /translation="MFRGASSISIDSKGRIAIPTRYRAELLEKHHGILVCTVDIRQPC
                     LLLYPLHEWEMVEQKLLALSNFDPVQRRIQRVMQGFATECEMDSAGRILLSPTLRQHA
                     QLEQQIMLVGQLNKFEIWQDKQWQAQIAEDLAFGSSAEILDCEALKNLSL"
     gene            4842..5780
                     /gene="rsmH"
                     /locus_tag="PXD81_001871"
     CDS             4842..5780
                     /gene="rsmH"
                     /locus_tag="PXD81_001871"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009874743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HDL5537876.1"
                     /translation="MSENPKHITVLLHEAVDGLAIKPNGIYIDGTFGRGGHSRLILSK
                     LGENGRLIATDRDPRAIAEAQTISDPRFQIEHTAFSAIPELCERMGLVGKIDGILLDL
                     GVSSPQLDEAERGFSFMRDGPLDMRMDTTKGLSAAEWLAQVSAEDLAWVLKEFGEERF
                     AKRIAQAVVSYNKSANEKISRTLQLAQIIADAVPFKDKHKHPATRSFQAIRIFINGEL
                     DELEKALNSALTVLAPEGRLSIISFHSLEDRMVKQFVRKHSKGMDVPRGLPILEAELT
                     KNIPLKTIGKAIMPSEAEIEANARSRSAVLRIAEKR"
     gene            5799..6110
                     /gene="ftsL"
                     /locus_tag="PXD81_001872"
     CDS             5799..6110
                     /gene="ftsL"
                     /locus_tag="PXD81_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HDL5537877.1"
                     /translation="MSNERYPLHQIIIDDIFSHNKVALLLLIGVVISAVATIWITHQT
                     RLSVSEQGQLIQANQKLESQYTNLQLEENSRSSRARVDAAAKSFGLQPIKKEQEIILV
                     E"
     gene            6114..>6556
                     /locus_tag="PXD81_001873"
     CDS             6114..>6556
                     /locus_tag="PXD81_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsI"
                     /protein_id="HDL5537878.1"
                     /translation="MVKSVKLKRKQEAPVAKAEPQQAEGKKTPSFLPTRFRVVVFFML
                     LMVGGLLAQTAYIQLFDSERLIKEANNRSLRTKELQFTRGRILDRNGRFLSISVPMYS
                     LTLDPREYFDTKLRRSNESWRALAIEMETSKGKIEANVNKFIEKK"
BASE COUNT         2076 a         1266 c         1466 g         1748 t
ORIGIN      
        1 attaaatgcc ataaggggaa ttttatgttt aaaagaacat taatcggtct agccgtgtta
       61 ggtgcaagtg ttgctcaagc ggatgtatta accagtatta agcctttagg ctttattgcc
      121 agtgccatta ccgaaggggt aactgaaact aaagtattat taccggtaac tgcctctcca
      181 cacgattaca gcttaaaacc ttcagatgtg actcaattac aatctgctca attagtggtt
      241 tgggtgggtg acggcttgga aggcttttta gaaaaaagta ttgaaaaact gccgagagaa
      301 aaagtattaa cccttgaaga tgttgatgcg attgatgata ttgtcgataa agcaagcaaa
      361 aaagaagata aagacgatga tcacgatcat aaacatgaac ataagcacga acacaagcac
      421 gagcataaac acgaagggca taatcacgaa ggacataacc acgatcatca cggacatagc
      481 cacgatgaag attggcatat ttggctctca agcgaggcaa gtgagctggc agcagagcaa
      541 attgcggcac gtttaagcga acaaatgcct gaacacaaag cgaaaattgc ggaaaatctt
      601 gcaaaattta aagcaaatct aaccgctaaa aaagccgaaa ttgcgaaaca attagagccg
      661 gtaaaagcca aaggttacta cacattccac gatgcttacg gctattttga aagagaatat
      721 ggtttaaatt cattgggttc atttaccatt aacccaactg ttgccccggg tgcaaaaaca
      781 cttacagcaa tcaagaaaaa cattgcgaaa aacaaagcac aatgtttatt tgccgagccg
      841 caatttacgc cgaaagtaat tgaaacctta gcaaaagaaa ctaaagtaaa tgttggccaa
      901 ttagacccac tcggtgcaaa agttcagtta agtgcgactg cttaccctga atttttacaa
      961 gttttggctg acgaatttag ccaatgttta acaaaataag ttaaaaatcc cccatttaaa
     1021 tataaatggg ggattttgcg attacatcac ttaagctttt tttaagaatt ctgatttcaa
     1081 cataatttta ccgcaatcga cattatggtc accttgagct aaacggattc ctttgaattt
     1141 tgtacctttt ttcagtactt cagacgagcc ttttaatttt aaatctttga ttaataatac
     1201 atcgtccccg tcagataata aattgccatt actgtctttt acaattagca catcatcatc
     1261 ttgatccgtt tcttcgcccg tccattcata cgcacaatcc gggcaaacaa attgaattga
     1321 gtcgtggtaa gtattttcgc ttttacattc agggcaggtt gggtaaatca ttttaattcc
     1381 tcaaattaat aaagaaccag attatatacc gaaatccagc aaaacgctaa gcggtcatat
     1441 tttgccgatt ttttaccaat cgagctaaaa gtcagttaga atagccttaa ttacacaaaa
     1501 aggaggatac tatgatagtc gatccgaatt atcagcaaga attagaaaaa tacgaaaatc
     1561 cggtaccaag ccgagagttt attttaaata caattcgtga acataacgcc ccaatgagcc
     1621 gtgatgaatt attgtcggct tttcatattg acgatgaaga acgaatggaa ggcattcgcc
     1681 gccgtctgcg tgcaatggaa aatgacggtg aattggtgtt caccaaaggc aaacgctatg
     1741 cgttgccgga aaaaatggat ttaatcaaag gtacagttat cggccatcgt gacggttacg
     1801 gttttttgca agtggaagga cacgaaaaag gggcgactaa aagcgaagat tggtttattc
     1861 ccaacgccca aatggtaaag gtaatgcacg gtgactttgt gctggcacaa ccaaacggca
     1921 cagaccgcag aggtcggaaa gaagtccgca ttgtgcgagt gttggaggcc cgtaagaaac
     1981 agattgtcgg gcgttttttc cttgaaagcg gtatcggttt tgttgtgcct gatgacagcc
     2041 gcgttaacca agatattctc attccggatg aacaccgtat gggggcgaga atggggcaag
     2101 ttgtggtggt ggaattacag gaaagaaaag cgagttttag ccgcccggtg ggaatcatta
     2161 ctgaaattct aggcgataat ttagcgccgg gaatggaaat tgaaattgcc ctacgcaatc
     2221 acgatattcc gcacatttgg ccggaagggg tagaaaaaca aatccgccaa tttagtagcg
     2281 aagaagtgcc ggaagaagcc aagcaagggc gggtggattt gcgagatctg ccactgatta
     2341 ccattgacgg cgaagatgcc cgagatttcg atgatgctgt atttgccaaa aaagagggca
     2401 acggctggcg gttatgggtg gcgattgccg atgtaagtta ttatgttcgc ccaaaaaccg
     2461 cattagattt ggaagcccta aatcgtggta actcggtcta tttcccgaat cgggttgtgc
     2521 caatgttgcc ggaagtgctt tccaatggct tgtgttcact caatccacag gttgacaggc
     2581 tctgcttggt ggcggaaatg tcggtttcca gcaaagggga attacaagat tatcaattct
     2641 acgaagcggt aatgaactct cacgcccgtt taacttacac taaagtgtgg aaaatgttgg
     2701 aaggtgatga agccttgcgt gaacgctatg ctccattagt gccgcatatt gaagaactct
     2761 atgcaatgtt ccaaacctta atgaaagctc gccataaacg aggggcgatt gaatttgaaa
     2821 ccattgaaaa tcaatttatt ttcaatccgc aagggcgaat tgagcgaatt gagccgttaa
     2881 tccgcaacga tgctcataaa ttgattgaag aatgtatgat tttagccaat attgctgccg
     2941 ctcgttttgt ggaaaaagcg aacgaaccgg cgttatatcg gattcacgat aagccgagtg
     3001 aagaaaaatt gctcagtttt aaatcctttg tgcgtgagtg tggcttaacc tgggacgtag
     3061 gcttagatcc aaccccaaaa gattacggtg tactactgga gcaaattgcc gaacgtgccg
     3121 ataaagagtt gattcaaacg atgttgctcc gctcgctgaa acaagcagtg tatgcggcgg
     3181 ataatatcgg gcatttcggc ttggcattaa ccgaatatgc tcattttacc tcgccgattc
     3241 gccgttaccc ggatttactg ttacaccgtg caattaaata tctgatcgaa aaaggcaaag
     3301 gcaacactcg ctactatacc gatggcggtg gttatcacta caagttagat gatatggacg
     3361 aattcggcga taaatgttct gccaccgaac gccgagctga tgaggcaacc cgagaagtgg
     3421 cagattggct gaaatgcgaa tttatgcaag atcatcttgg cgaagagttt gatggcgtga
     3481 tttcgagtgt taccggcttt ggcttatttg taaaactcaa tgagctttta attgacggat
     3541 tggttcatat ttcaacgctg gataatgatt attaccacta tgatgcggac cgacaacgtt
     3601 taatcggtgg cagtgggatc atctatcgtt taggcgatgc agttagagta aaagtaatca
     3661 atgtgaattt agacgagaaa aaaattgatt ttgagttact caccggtaat aagaaaaccg
     3721 gcaaaaccgc caagaaaaaa gcaaaagcga cagaggttcc tgttttcaaa gagcctaaaa
     3781 cggtaaaaaa agccaaaaaa gtcgctgaga aaaaaccggc aaaatctgca aaaaaacgga
     3841 caaaaacgac cgctagtaaa tcaaggaaaa aatagcaaat ggattacaaa aaacaaattt
     3901 tattcagcct tttatttgct ttagtaattg caataataag ctattttttg tttacctata
     3961 tcccgtttga ttgtagcgat ctgagcaaac tcaacgattc acaacgttta gagtgccgaa
     4021 ctgccgaatt tctgggcgtt tattactggc tgccgcacac taccacgctg tttttcggct
     4081 cggtgttatt atattgggcg gtttatggca tgatttcatt agtgcaagca gtgctcaaac
     4141 gctaacaagc agttttgttt ttgcaaaaag ttgcaaatct catctgaata atccttccaa
     4201 gatcaaaggt ctatcaaaaa agatagacct ttattgtgta aataaactaa aactccttga
     4261 tagatgtatc acatatctat aaaattccag caattaagtg ggaaaaagtg gtaaattgtg
     4321 gaacttttcc ttaaatttaa cctcatttaa aaaggtaaat cagtaaaatg tttcgtggtg
     4381 caagttcaat cagcattgat agcaaagggc ggatcgctat tccgacccgt tatcgtgccg
     4441 agctattgga aaaacatcac gggatcttag tttgcactgt ggatatccgc cagccttgtt
     4501 tgttgcttta cccacttcac gaatgggaaa tggttgaaca gaaattgttg gcactatcta
     4561 attttgaccc tgttcaacgc cgtattcagc gagtaatgca aggctttgct accgaatgtg
     4621 aaatggattc ggcagggcga atactgctta gccctacact tcgccaacac gcacaattag
     4681 agcaacaaat tatgctggtt ggacaattaa acaaatttga aatttggcaa gataaacaat
     4741 ggcaagcaca aattgccgaa gatcttgctt ttggcagttc ggcagaaatt ttagactgcg
     4801 aagcattgaa aaacctgtct ctctagtaat tggaaattga gatgagcgag aacccaaaac
     4861 atattacagt attattgcac gaagcggttg acggcttggc aattaagcct aacggtatct
     4921 atattgacgg tactttcggg cgtggcgggc attctcgctt gattttatcc aaactgggcg
     4981 aaaatgggcg attaatcgca actgatcgtg acccacgagc gatagccgaa gctcaaacga
     5041 ttagtgaccc tcgttttcaa attgaacata cggcattttc tgccattccc gaactgtgtg
     5101 aacgaatggg gttggtgggc aaaattgacg gtattttatt ggatttaggt gtgtcttccc
     5161 cgcagcttga cgaagccgaa cgaggcttta gttttatgcg tgacgggccg cttgatatgc
     5221 gaatggatac caccaaaggc ttatcggcag ccgagtggct tgcccaagtt tcggcagaag
     5281 atttagcttg ggtattgaaa gaatttggcg aagagcgttt tgccaaacgg attgcacaag
     5341 cggtcgtttc ttacaataaa tctgcaaatg aaaaaatcag ccgcactttg cagcttgcgc
     5401 aaattattgc ggatgcggtg ccgtttaaag ataagcacaa acaccctgca acccgttcgt
     5461 tccaagcaat tcgcattttt atcaatggtg aattagacga gctggaaaaa gctttaaatt
     5521 ccgcattaac ggtgttagcc cctgaagggc gtttgtcgat tatcagcttt cattctctag
     5581 aagaccgaat ggtgaaacag tttgtgcgta agcatagtaa aggaatggac gtaccccgtg
     5641 gtttaccgat tttagaagca gagctaacca aaaatattcc gctgaaaacc attggcaaag
     5701 cgattatgcc gagcgaagcc gaaattgaag caaatgcccg ctcacgcagt gcggtattgc
     5761 ggattgccga aaaacgctaa cagtaaatag ggaagaatat gtcgaacgaa cgttatccgt
     5821 tacatcaaat catcattgat gatatcttta gccataacaa agtggctctt ttattgttga
     5881 ttggtgtagt gatttcagca gtggctacga tttggataac ccaccagact cgtttatcgg
     5941 tgagcgaaca agggcaactg attcaagcaa accaaaagtt ggaaagccaa tatactaatc
     6001 tgcaattaga agaaaacagc agaagcagtc gggcaagagt tgatgccgcc gctaaatcgt
     6061 tcggattaca gccgattaaa aaagagcaag aaattatttt agttgaataa aaaatggtta
     6121 aatcagttaa attaaaacgt aagcaagaag cccctgtcgc aaaagcagag cctcaacaag
     6181 cagaaggtaa aaaaacaccg agttttttac ctactcgctt tagagtggtt gtgtttttta
     6241 tgctattaat ggttggtggc ttactagcac aaaccgccta tattcagctt tttgactctg
     6301 aaagattaat taaagaggct aataaccgct cacttcgtac taaagaactc caatttaccc
     6361 gtggacgtat tttagatcgt aacgggcgtt ttctctctat tagtgtgcca atgtactcat
     6421 taacacttga cccgagagaa tactttgata ctaagctgcg tcgttctaac gaaagctggc
     6481 gtgccttagc gattgaaatg gaaacatcaa aaggtaaaat tgaggcaaac gttaataaat
     6541 ttattgaaaa gaaaaa
//
LOCUS       DANLYI010000141         6551 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.142, whole genome shotgun sequence.
ACCESSION   DANLYI010000141 DANLYI010000000
VERSION     DANLYI010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 6551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6551
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.142"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            <1..1414
                     /gene="aspA"
                     /locus_tag="PXD81_001874"
     CDS             <1..1414
                     /gene="aspA"
                     /locus_tag="PXD81_001874"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005608308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HDL5537879.1"
                     /translation="IEHDLLGEREVPNSAYWGIHTLRAIENFNISNVTISDVPEFVRG
                     MVMVKKATALANGELGAIPKKIAKAIVAACDEVLVKGRCLDQFPSDVYQGGAGTSVNM
                     NTNEVIANLALEILGEAKGKYDIVNPMDHVNASQSTNDAYPTGFRIAVYNSITKLINK
                     ITYLQTAFENKATEFSDVLKMGRTQLQDAVPMTLGQEFKAFAVLLDEEVRNLKRTAEL
                     LLEVNLGATAIGTGLNTPEGYSTLAVKYLAEVTQLPCSLSENLIEATSDCGAYVMVHG
                     ALKRTAVKLSKVCNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPEVVNQVC
                     FKVIGNDTTVTFAAEAGQLQLNVMEPVIGQAMFESIELLSNACINLRDKCIDGITANR
                     DICQNYVFSSIGIVTYLNPFIGHHNGDIVGKICATTGKSVREVVLERGLLTEEQLDDI
                     LSIENLMNPTYKAKRFTEEE"
     gene            complement(1489..3507)
                     /gene="rep"
                     /locus_tag="PXD81_001875"
     CDS             complement(1489..3507)
                     /gene="rep"
                     /locus_tag="PXD81_001875"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005600347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HDL5537880.1"
                     /translation="MKLNSQQQQAVEYTKGACLVLAGAGSGKTRVIINKIAHLIAHCG
                     YSPKQIAAVTFTNKAAREMRERVDHSIGKENSKGLTISTFHTLGFEILKREYKLLGFK
                     SGMTLFDEQDQIALLKHLLPENVTEDKDLLKALIATISNWKNDLLSPERVLTRVRDER
                     ERVFSHFYQLYQNQLKAYNALDFDDLIMLPVLLFRQFPEAKSRWQQKIRYLLVDEYQD
                     TNTSQYELIKLIVGNEPNFTVVGDDDQSIYSWRGAKPENMQRLREDFDLQVIKLEQNY
                     RSSQRILHCANILIDNNDHLFSKRLFSELGQGEKLQVIEAKNEEHEAERIVGELIAHR
                     FSKKTKYKDYAILYRGNHQSRLLEKLLMQNRIPYKISGGTSFFARAEIKDMMAYLRLV
                     VNQDDDAAFLRIVNTPKREIGAATLEKLGILANEKQVSLFEAIFDFELIQRLTPKPYQ
                     ALQDFARWIVEIADQADRSDPIEAVKDLLAKIQYEAYLYEMAANPKVAEMQSRNVQTL
                     FEWVQAMLEGDEIEPPMNLVQVVNRLTLRDMLERGEEDDEADQVQLMTLHASKGLEFP
                     HVFLIGMEEGILPHQTSLDEDNVEEERRLAYVGITRAQQTLTFSLCKERRQYGEIIKP
                     EPSRFLLELPQDDLEWESEKPRMTEQEKEAKSSANIANLMAMLKANKK"
     gene            3755..4870
                     /gene="potA"
                     /locus_tag="PXD81_001876"
     CDS             3755..4870
                     /gene="potA"
                     /locus_tag="PXD81_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HDL5537881.1"
                     /translation="MEKVEQKAIIELKNVTKSYGSKTILKNLNLTINDGEFLTILGPS
                     GCGKTTALRLIAGFEDLTEGSIILDGQDVSNVPAEQRPVNTVFQSYALFPHMTIFENV
                     AFGLRMQKVPNAEIEPRVMEALRMVRLEDRAQQKPAQLSGGQQQRIAIARAVVNKPKV
                     LLLDESLSALDYKLRKEMQNELKALQRQLGITFIFVTHDQEEALTMSDRIIVMNAGKI
                     AQDGTPREIYEEPSNLFVARFIGEINVFDATVIERVDATNVKANVEGRICNIKVEDLE
                     VQPNQKLKVLLRPEDIVIEELDENESSKAIIGHIIDRNYKGMTLESSVRLDHNGMNVL
                     VSEFFNEDDPNIDHEVGQKVALTWYEGWEVVLNDEGE"
     gene            4857..5720
                     /gene="potB"
                     /locus_tag="PXD81_001877"
     CDS             4857..5720
                     /gene="potB"
                     /locus_tag="PXD81_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005698572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HDL5537882.1"
                     /translation="MKANNKFQNGAVATIFGWLLLFVLVPNVLVLIISFLTENRQSQY
                     FVDFAFSFDAYKSLFNDTYAAVLWNSLYMAGIATFFCLIIGYPFAFFIAKMPERIRPF
                     LLFLVVLPFWTNSLIRIYGIKIFLGVKGFLNESLMALGIISEPMRLLNSEIAIIIGLV
                     YILLPFMILPLYSSIEKIDNRLLEAAKDLGANAFQRFIKVTIPLTMPGIVAGCLLVLL
                     PAMGMFYVADLLGGGKTPLVGNIIKSLFLNTNQFALGSAVSIALTILMALMLYVYYRA
                     NKLLNKKVELE"
     gene            5720..6487
                     /gene="potC"
                     /locus_tag="PXD81_001878"
     CDS             5720..6487
                     /gene="potC"
                     /locus_tag="PXD81_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007525438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HDL5537883.1"
                     /translation="MKKMFRNLFMLVVFAYLYIPIIILVINSFNEDRFGLNWKGFTWN
                     WYERLFSNDTLVQATLNSLTIAFFAATFSTILGALTSIALYRYRFRGKKLVGGMLFVV
                     MMSPDIVMAVSMLVLFMILGIKLGFISLLIAHITFCLPYVVIAISSRLSDFDGKMLEA
                     AKDLGASEVTILRKIILPLALPSIISGWLLSFTISLDDVIVSSFVTSPSYEVLPLKIF
                     SLVKTGVTPEVNALATIMIIFSLTLVVLSQLVVKKKV"
BASE COUNT         1911 a         1311 c         1300 g         2029 t
ORIGIN      
        1 tattgaacac gatttacttg gtgaaagaga agtacctaat agtgcttatt ggggcattca
       61 tacattaaga gcgattgaga attttaatat ctcaaatgtc accatttctg atgtacctga
      121 atttgtgcgt ggcatggtaa tggtaaaaaa agctactgcg ttggcaaatg gtgaattggg
      181 tgcaatcccg aaaaaaattg ccaaagctat tgtggcagcc tgtgatgaag tattagtgaa
      241 aggacgctgt ctcgatcaat tcccttcaga tgtttatcaa ggtggagccg gcacatcggt
      301 gaatatgaat accaatgaag tgattgccaa ccttgcatta gaaattttgg gcgaggcaaa
      361 aggtaaatat gatattgtca atccaatgga tcacgttaat gcaagccaat ccaccaatga
      421 tgcctatcca accggtttcc gcatcgcggt ttataacagt atcactaaat taatcaataa
      481 aatcacttac ctacaaactg catttgaaaa taaagccact gagttttccg atgtactaaa
      541 aatgggaaga acgcagttac aagatgccgt gccaatgacg ctcggtcagg aatttaaagc
      601 ttttgccgta ttattagatg aagaggttcg taaccttaaa cggacggcag aattgcttct
      661 tgaagtgaat ttaggcgcaa ccgctatcgg tactggtctg aatacgccag agggttactc
      721 tactcttgca gttaaatatt tagccgaagt cacgcaactg ccttgctcac tgtctgaaaa
      781 tctcattgag gcaacatctg actgtggtgc ttatgtaatg gttcacggtg cattaaaacg
      841 caccgccgtc aaactttcta aagtgtgtaa tgacttacga ttactttcat caggcccacg
      901 tgccggctta aatgaaatta atcttccgga attacaagcc ggctcatcta ttatgccggc
      961 aaaagtaaac cctgttgtac ctgaagtcgt aaaccaagtg tgctttaaag tgattggtaa
     1021 cgacaccacc gttacttttg cagcagaggc cggtcaatta cagcttaacg taatggagcc
     1081 ggttatcggg caagcaatgt ttgaatctat cgagctgctt tccaatgcat gtattaatct
     1141 acgtgataaa tgtattgacg gtattaccgc taaccgtgac atctgccaaa actatgtgtt
     1201 cagctctatt ggtattgtga cctacctcaa tccatttatc ggccaccata atggtgatat
     1261 tgtcggcaaa atttgtgcaa ccaccggcaa aagtgtacga gaagttgtgc ttgagcgagg
     1321 cttactcacc gaagaacaac tcgatgatat tctctcgatt gagaacttaa tgaatccgac
     1381 ttataaagcc aaacgcttta ccgaagagga ataatcattg tttacaagcg gtctgattct
     1441 ggcaaaaaat tgcaaatttt taccaaaatc agaccgcttg ttatcccatt atttcttatt
     1501 tgctttcagc atcgccatta aatttgcaat atttgcagaa gattttgcct ctttttcctg
     1561 ttcggtcatt cttggctttt cactttccca ctccaaatca tcttgtggca attccagtaa
     1621 aaaccggctc ggctcaggtt tgatgatttc gccatattgg cgacgttctt tacacagtga
     1681 aaacgtgagc gtttgttggg ctcgggtaat gccgacataa gccaaacgcc gctcttcttc
     1741 tacattgtcc tcatcaaggc tggtttggtg tggcagaatc ccctcttcca taccgattaa
     1801 gaaaacgtgc ggaaattcca accctttcga ggcgtgaaga gtcattagtt gaacttgatc
     1861 ggcttcatcg tcctcttcgc cacgttcgag catatctcgc aaggttaaac ggtttaccac
     1921 ttgcaccaaa ttcatcggag gctctatttc atcgccctct aacatcgctt gcacccattc
     1981 aaacaaggtc tgcacattac ggctttgcat ttccgctact tttgggttcg ccgccatttc
     2041 atacaaatag gcttcatact gaattttcgc caacagatct tttactgctt caatcggatc
     2101 ggatctatcg gcttgatcgg caatttccac aatccagcgg gcaaaatcct gtaaggcttg
     2161 gtaaggcttt ggcgtgaggc gttgtatgag ttcaaaatca aaaatcgcct caaacaaact
     2221 gacctgcttt tcattagcaa gtatgcctaa tttttctagc gttgccgccc caatttcccg
     2281 tttcggcgta ttcacaatac gcaagaaagc tgcatcatcg tcttgattca ctaccaaccg
     2341 cagatacgcc atcatatctt tgatttccgc tcgagcaaaa aatgacgtac cacccgaaat
     2401 tttatacgga atgcggtttt gcattagcaa tttttccaac aagcgggatt gatggttgcc
     2461 tcgatacaaa attgcataat ctttgtattt ggtctttttc gagaaacgat gggcaatcag
     2521 ctcgcccaca atgcgttccg cttcgtgttc ctcatttttt gcctcaatga cctgtaattt
     2581 ttccccttgc cccagttctg aaaacagccg cttgctaaac aagtgatcgt tattatcaat
     2641 cagaatattg gcacaatgca gaatccgctg gctggaacgg taattttgct caagtttaat
     2701 cacttgcaaa tcaaaatctt cccgtaaccg ttgcatattt tcaggcttcg ccccacgcca
     2761 agagtaaatt gattggtcat catcgcccac gaccgtaaag tttggctcgt tgccgacaat
     2821 cagcttaatt aactcatatt ggctggtgtt ggtgtcttga tattcatcaa ctaacaaata
     2881 gcgaattttc tgttgccaac gagattttgc ttcgggaaat tggcggaaca gcaacaccgg
     2941 caacataatc aaatcatcaa aatccaacgc attataagcc ttaagttgat tctggtagag
     3001 ctgataaaaa tgtgaaaaca ctcgctcacg ttcatcacgc acacgagtaa gtaccctttc
     3061 aggcgaaagc aaatcgtttt tccagttaga aatagtggca attaaggctt tcagcagatc
     3121 cttatcttcc gtcacatttt caggcaataa atgtttcagc aaagcaattt gatcctgctc
     3181 atcaaacaag gtcatacccg atttaaaacc gagcaattta tattcgcgtt ttaaaatttc
     3241 aaacccaagt gtgtgaaaag tcgaaatggt taaacctttg ctgttttctt tgccgataga
     3301 atgatccacc cgttcacgca tttcccgagc agctttgttg gtaaaggtta ctgctgcaat
     3361 ctgcttaggc gaatagccac aatgtgcaat taaatgtgcg attttattaa taatcacccg
     3421 agtcttaccc gagccggcac cagccagcac aaggcaagcc cctttggtat attctactgc
     3481 ttgttgttgt tgagaattga gtttcattga atctattcaa aagtaatttt gacaactatt
     3541 ttaacggaaa gaaaacaagc ggtcgaattt tcaggaaaat ttgcaaaaaa ctttgaaaat
     3601 ccgaccgctt gttcatcaga gaaaaatccc caaaacgcaa atttttcctt tcaatcttgc
     3661 ccctttagct atatcattat aagttgattt tttaggggca aatgcctgat tttataactc
     3721 cttttctttt taatttaagg gaaaaaaaca gtagatggaa aaagtagaac agaaagctat
     3781 cattgagctt aaaaatgtaa caaaaagcta cggtagtaaa acgattctta aaaatttaaa
     3841 tttaaccatt aacgatggtg aatttttaac aattttaggc ccttccggtt gtggtaaaac
     3901 aactgcgtta cgtttaattg ccggttttga agatttaact gaaggttcaa ttattcttga
     3961 tgggcaagat gtttctaacg ttccggcgga gcaacgcccg gtaaacaccg tattccaaag
     4021 ctatgccctt ttcccacata tgacgatttt tgaaaacgtg gctttcgggc tgcgtatgca
     4081 aaaagtgcca aatgctgaaa ttgagccacg tgtgatggaa gcattacgaa tggtgcgttt
     4141 agaagaccgc gctcaacaaa aaccggctca gctttccggt ggtcagcaac aacgtattgc
     4201 aattgcccgt gcggtggtaa ataaaccgaa agtattgttg ttagacgaat cgctttctgc
     4261 gttagattat aaattgcgta aagagatgca aaatgaatta aaagcattgc aacgtcaatt
     4321 aggcattacc tttatttttg tgacccacga tcaggaagaa gccttaacca tgtctgatcg
     4381 tattatcgta atgaacgctg gtaaaatcgc tcaagacggt acaccacgtg agatttacga
     4441 agagccaagt aacttatttg tagctcgttt catcggcgaa attaacgttt ttgatgcaac
     4501 tgtaattgag cgtgttgatg caactaatgt aaaagcaaac gtagaaggtc gcatttgcaa
     4561 tatcaaagtg gaagatttag aggttcagcc gaaccaaaaa ttaaaagtat tgttacgccc
     4621 tgaagatatt gtgattgagg agttagacga aaacgaaagc tcgaaagcaa ttatcggtca
     4681 tattatcgac cgtaactaca aaggtatgac attagagtct agcgtgagat tagatcataa
     4741 cggtatgaat gtattggtta gcgagttctt caatgaagac gatccgaaca ttgaccacga
     4801 agtgggacaa aaagttgctc ttacttggta tgaaggctgg gaggttgtgt taaacgatga
     4861 aggcgaataa caaattccaa aatggtgcgg tcgcaactat tttcggttgg ttattacttt
     4921 tcgtattagt gccgaacgta ctcgttttaa tcatcagctt cttaacagaa aaccgtcaaa
     4981 gtcagtattt tgtggacttt gcatttagtt ttgatgctta taaatcactg tttaatgaca
     5041 cctatgcagc agtattgtgg aactcgctat atatggcggg cattgccacc tttttctgtt
     5101 tgatcatcgg ctatcccttt gcatttttta ttgccaaaat gccggaaaga attcgtccgt
     5161 ttttactgtt tttagtagtt ctaccgtttt ggactaactc actgattcgt atttatggca
     5221 ttaagatttt cttaggcgta aaaggctttt taaatgaaag cttgatggct ttaggtataa
     5281 tcagcgagcc aatgcgttta ttaaactcag aaattgcgat tattattggt ttagtttaca
     5341 ttctattacc ttttatgatt ctaccgcttt attcatcaat cgagaaaatt gataaccgct
     5401 tactagaagc tgcaaaagac ttaggtgcaa acgccttcca gcgttttatt aaagtgacta
     5461 ttccattaac aatgccgggc attgtcgcag gttgtttatt agtattactt ccggcaatgg
     5521 gtatgttcta tgtggcagat ttattaggcg gcggtaaaac accgttagta ggtaacatta
     5581 ttaagagctt gttcttaaat accaaccaat ttgcattagg ttcagcagta agtattgcat
     5641 taactatctt aatggcatta atgctttatg tttactatcg tgcaaacaaa ttattaaata
     5701 aaaaggtgga gcttgaataa tgaaaaagat gtttagaaac ctttttatgt tggttgtttt
     5761 tgcatatctc tatattccaa ttatcatttt agtgatcaac tcctttaatg aagaccgctt
     5821 tggattaaat tggaaaggct ttacttggaa ttggtatgag cgtttattca gtaatgatac
     5881 gcttgtacaa gcaacattga actcattaac tattgcgttc tttgcggcaa cattctcaac
     5941 cattttaggg gcattaacct ctattgcgct ttaccgttac cgtttccgtg gtaaaaagct
     6001 agtgggcgga atgttattcg tggtaatgat gtcgcctgat atcgtaatgg cggtatctat
     6061 gctcgtatta tttatgatct taggtatcaa gctaggcttt atttcgctat taattgcaca
     6121 cattactttc tgtttgcctt atgtggtaat tgcaatttcc tcacgcctca gcgattttga
     6181 cggcaaaatg ctagaagctg caaaagacct aggtgcaagt gaagtgacta ttttgcgtaa
     6241 aattatttta ccactggcat taccttcgat tatttcaggc tggttgttaa gctttacgat
     6301 ttcgttagat gacgttattg tttcatcttt cgtgacaagc ccaagctacg aagttttacc
     6361 actgaaaatt ttctcattgg ttaaaacagg tgtaacacct gaagtgaatg cactagcaac
     6421 tattatgatt attttctcat taacattagt ggtattaagc cagttagtag tgaagaaaaa
     6481 agtctaaaaa tcactaaaaa tcgtatacat aaatcagcac tttaaagagt attaaagtgc
     6541 cgattttttt a
//
LOCUS       DANLYI010000142         6531 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.143, whole genome shotgun sequence.
ACCESSION   DANLYI010000142 DANLYI010000000
VERSION     DANLYI010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 6531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6531
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.143"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            183..1100
                     /locus_tag="PXD81_001879"
                     /pseudo
     CDS             183..1100
                     /locus_tag="PXD81_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014167835.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            complement(1102..1770)
                     /locus_tag="PXD81_001880"
     CDS             complement(1102..1770)
                     /locus_tag="PXD81_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HDL5537884.1"
                     /translation="MKYLYALIFSLFFSSVYAAERQLQCKVVGISDGDTLTCLVNKTP
                     LKIRLQHIDAPEQAQPYGNKAKQALAQFVFQQNITAIVSGYDRYQRLLATLYLNDKNI
                     NLALVENGMAWAYTTKREYIEAQDQAQQAKIGLWQDKFPINPSQWRKTHTSTLPQAVK
                     KPENIANQPPTIDCQIKLSCNQIGNYAQAKRYFQQCGWKELDGNNDSIPCNKLYRQEQ
                     KNRN"
     gene            complement(1814..3181)
                     /locus_tag="PXD81_001881"
     CDS             complement(1814..3181)
                     /locus_tag="PXD81_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012263108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HDL5537885.1"
                     /translation="MSIDTILNQINSFVWGPPLLILISGVGLYLTFSLKGIQLSQLGQ
                     AFRYMFKPEKGKGQSGDISAFAALSTALAATIGTGNIVGVATAIHSGGPGALFWMWLI
                     ALFGMATKYAEGLLAIKYRTKDRAGFVAGGPMYYIELGMGKKWRWLAKAFAFFGVLVA
                     LLGIGTFPQVNGITHALESTFDVPIIFSAIILTLIVTAIILGGVQRISKIATIVVPFM
                     AIGYVIAALSVILLNMEKVPQAIITIVHAAFNPQAALGGAVGFTVMQAIQLGVARGIF
                     SNESGLGSAPIAAAAAQTKEPVRQGLISMTGTFLDTIIVCTMTGLVIVISGAWAGDVK
                     GAELTTTAFTQGLNSSFGAIIVTVGLVFFAFTTILGWCYYGERCFVYLTRGRTQYIKL
                     YRLLFIALIASAPFLQLDTIWTLADIVNGLMAFPNLIALIALRKVIISETKDFFARLK
                     TGKAE"
     regulatory      complement(3300..3395)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            complement(3566..4618)
                     /locus_tag="PXD81_001882"
     CDS             complement(3566..4618)
                     /locus_tag="PXD81_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDL5537886.1"
                     /translation="MTHSTKTLVAIASIIIILAGIKLSNEIVVPFLLSLFIAIICSPM
                     IKFMTKRKVPLWLAITILLIVFILGFSFLAGIINNTLQEFTNAIPQYKIQLSSRIQVI
                     LELAHRWHIPLTLSQEEIMSKFDPSSIMNLVSKLLMGLSGALSNIFVIFLVVIFMLLE
                     MPVAKYKLALALSPDDDLSNEKEYINQVLEGVIGYLGVKTFVSLLTGGLVWILLEILG
                     VEYAILWAILTFLLNYVPNIGSILAGIPIVIQALVLNGFSIGLGTLIGIVVINMVVGN
                     ALEPKLMGKKLGLSTLVVFLSLLFWGWLLGTVGMLLSVPLTMACKVALESNKATQKYA
                     VLFSDLEEVPKEQKYR"
     gene            complement(4665..5090)
                     /gene="ruvX"
                     /locus_tag="PXD81_001883"
     CDS             complement(4665..5090)
                     /gene="ruvX"
                     /locus_tag="PXD81_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HDL5537887.1"
                     /translation="MTETTTKTVIAFDFGTNSIGCAVGQSITGTAQGLTTFKAQDGIP
                     NWEEIGKLLQDWKPDMLVVGLPLNMDGTEQPLTQRARKFANRLNGRFNLPVELQDERL
                     TTVEAKAEIFSRGGYKALKKDKVDSISACLILESWFENH"
     gene            complement(5090..5650)
                     /locus_tag="PXD81_001884"
     CDS             complement(5090..5650)
                     /locus_tag="PXD81_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HDL5537888.1"
                     /translation="MLENLQGKFLIATPEMDDDYFERTVVYICEHNPQGAMGVVINTP
                     TDLSVLELITRMDFQMANQRDYKKDQMVLSGGPVNQDRGFIIHTKTANDFIHTYPITD
                     KITLTTSGDVLDSFGTLASPEKFIVCLGCSTWRGSQLEQEIAQNYWIVSDANEKTLFE
                     TGYLERWAEAYEMMGLSSIPSRAGRA"
     gene            complement(5650..6381)
                     /gene="rsmE"
                     /locus_tag="PXD81_001885"
     CDS             complement(5650..6381)
                     /gene="rsmE"
                     /locus_tag="PXD81_001885"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007524366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HDL5537889.1"
                     /translation="MRIPRIYQPQPLAGLQSCVLSEDAANHVGRVLRMKQGEQIILFD
                     GSNHVFHATLQAVEKKQIIAKIDSSELDDRESNLPIHLGQVISRGDRMEFTIQKSVEL
                     GVKTITPLWSERCGVKLNDERQDKKLQQWQKIAIAACEQCGRNEIPEIRPIMKLTDWC
                     KEQDDMLKLNLHPRAKYTIRELPNVPKAGVRLLIGSEGGLSAEEIAMTESEGFTEVLL
                     GKRVLRTETASLAAITALQVCFGDI"
BASE COUNT         1921 a         1308 c         1339 g         1963 t
ORIGIN      
        1 tttatcgtca actgaatgaa gagcagatta ttaaaggagc aaaagcccat tcgcatttta
       61 tggaaacttt atatggtatt acaacgttaa aatcattagg attggaaaat aaacgacaag
      121 aacagtggat gtcgctcaat gcggatgctt ttaacacatc aattaaggta actaaatttg
      181 atatgctgtt ttccggtatt cataccttta tttctaccat cgaacagatt ttaattcttt
      241 ggattggggc gacaatgatg attgaaaatg tgatgacatt aggaatgttt gtcgctttta
      301 atgcctatcg aggctctttc tcttcacgaa tggggaattt aattgatatt atttttagcc
      361 taaaaattct ttcattacat agagagcgga ttgcagacat tgctttaaat gaagtcgaag
      421 atgaaagtaa atatcaagaa ctcagatttg aacataacca aacagatata gaagttaaag
      481 acctttgttt caaatacgat cctttttcta aaaatgtgct tgaaaatgtc aatttagtga
      541 tcagaagtgg ggaaagtgtt gctattaccg caccatcagg ttatggcaaa acaacgttat
      601 taaaattaat tgcaggttta ttaaaaccaa cctcaggttc tgtgtatttt aacggcattg
      661 atatttatca gttaggatta agtcaatatc ggagccaaat tgcttgcgta ttacaggaag
      721 ataaattctt ttctggttcc attttagaga atattgttag ttttgaaagt aattatgatc
      781 gagaatgggc tatcgaatgt agcaaacttg cgaatattca caatgatatt atggcaatgc
      841 cgatgaatta tgaaacctta ttaggagaat taggaaataa tttgtctggc ggacaaagat
      901 aacgattgtt tattgctcga gctttatata aaaagcctaa aattcttttg atggatgagg
      961 cgactagcca tttagatgaa gaaaatgaga agcttgtcaa tcaagctatt tcacgaatga
     1021 agataacaag gattattatt gctcatagac aatcgactat tcagtcggcg gatagaatta
     1081 ttgccctgaa tgacaggtaa attaattcct atttttctgt tcttgccgat aaagtttatt
     1141 gcacggaatg ctgtcgttgt tgccgtctag ctctttccag ccgcattgtt gaaagtagcg
     1201 ttttgcctgt gcgtaattgc cgatttggtt gcagcttaat ttaatttggc aatcaatcgt
     1261 tggtggctgg tttgcaatat tttccggttt tttgaccgct tgtggtaagg ttgaggtgtg
     1321 tgtttttcgc cattggctcg ggttaatcgg gaatttgtcc tgccataagc cgattttagc
     1381 ttgctgtgct tgatcttgtg cttcgatata ttcccgcttt gtggtgtaag cccacgccat
     1441 accgttttcc actaaggcta agttgatgtt tttatcgttt aagtagaggg tggcaagtaa
     1501 tcgttggtaa cgatcatagc ctgaaactat ggcagtaatg ttttgttgaa acacgaattg
     1561 agccagagct tgtttggctt tgttgccgta aggctgcgct tgttcggggg catcaatatg
     1621 ttgtagcctt atttttagtg gtgttttatt gaccaaacaa gtgagggtgt cgccatcgct
     1681 aatgccaacg actttgcatt gtagttggcg ttcggcggca tataccgatg agaaaaataa
     1741 gctaaaaatc agggcgtaaa gatatttcat tattaaaaaa gagagggcta ataagccctc
     1801 attctatcat tttttattcg gctttacctg ttttaaggcg agcgaagaaa tcttttgttt
     1861 cactaataat cactttgcgt aacgcaatca atgcgattaa attcgggaat gccattaagc
     1921 cgttcacgat gtctgccagc gtccaaattg tatcgagttg taaaaatgga gcagaggcga
     1981 tgagtgcaat aaacagcaag cggtataatt taatgtattg ggtgcgacct cgagtgagat
     2041 agacaaaaca gcgttcaccg tagtagcacc agcctaaaat ggtggtaaag gcaaagaaga
     2101 ctaaaccaac tgtcacaatt atcgcaccga atgagctgtt taaaccttga gtaaaagcgg
     2161 ttgtggtgag ttctgcgcct tttacatcgc ctgcccaagc ccctgaaatc acaattacta
     2221 agccggtcat tgtgcagacg ataatagtgt ctaagaacgt gccggtcatt gaaattaggc
     2281 cttgacgtac cggctctttg gtttgggcgg cagcggcggc gatgggggca gagcctaatc
     2341 ctgattcatt cgagaaaata ccacgtgcaa cccctaattg aatcgcttgc atgacggtga
     2401 atccgactgc tccgcctaat gccgcttgtg ggttgaaagc tgcgtgtaca atagtaataa
     2461 ttgcttgtgg gactttttcc atattcagta gaatgacgga taaagcggcg atgacatacc
     2521 caatagccat aaacggtaca acaatggttg caattttaga aatccgctgc acgccaccta
     2581 aaataatagc ggtaacgata agggttaaaa tgatcgcact gaagataatc ggcacatcaa
     2641 aggtgctttc taaggcgtgg gtaatgccat tcacttgggg gaacgtgcca ataccaagca
     2701 aagcgaccaa tacgccaaaa aacgcaaagg ctttagcaag ccaacgccat ttttttccca
     2761 ttcccagttc gatatagtac atcggaccac cagccacaaa tccggctcta tctttagttc
     2821 gatatttaat tgccaataag ccttcggcat atttcgtagc cataccgaat aaggcaatca
     2881 gccacatcca aaataatgct ccaggcccgc ccgaatgaat agcggttgcc acgccgacaa
     2941 tattccccgt gccgatagta gctgctaggg cagtggaaag ggctgcaaaa gcggaaatat
     3001 cgcccgattg ccctttgcct ttttccggtt tgaacatata gcgaaaggct tgccctagtt
     3061 ggcttaattg aatacctttg agtgaaaagg ttaaatataa ccctacgcct gaaattaaaa
     3121 tcaggagtgg cgggccccaa acaaaactgt tgatttgatt aagaatagtg tcgatagaca
     3181 tttgatgttc ctcgtaaaaa ctgcttacaa ggaaagaaaa ggaatgcaag agacaagcgg
     3241 tcaaaaaacg gaaatttttt gcaaagaatt agcaaaaaaa caccgcttgt atcgtataac
     3301 ttttctcccc tgtccttttg cctgagcgtt tcacacattg ctgtattttc gccttcggcg
     3361 gcggatttta atccgctctc tccaagggtt cgtccagtaa cagtccccgc agtgttcact
     3421 gcacctgaaa gagtttacct cgtcggtgaa atgttaattc atttctctcc agctaccttc
     3481 atccgaacaa tctaatttat tttataatgt aaaataaaaa agatttgcca aattctagca
     3541 actcttttgc ttccgtcaag tgaatttaac gatatttttg ttctttcggt acttcttcta
     3601 aatcgctaaa taatacggca tatttttggg tggctttatt ggattctaat gccactttac
     3661 acgccatcgt aagtggaaca gaaagtaaca ttcctacggt gccaagtaac cacccccaaa
     3721 atagaagtga gaggaacact acaagggtag aaagcccgag tttcttgccc attaatttag
     3781 gttcaagcgc attacccaca accatattaa tcacgacaat gccgattaaa gtgcctaatc
     3841 ctatagaaaa gccattgagt accaaggctt gaatcacaat tggaattccc gccaaaatag
     3901 agccgatatt cgggacgtag ttaagcagga aggttagaat tgcccataaa atcgcatatt
     3961 ctacgcctag aatttctagt aagatccaaa ctaaccctcc ggtgagtagg ctcacaaagg
     4021 ttttaactcc aaggtaacca atcacccctt caagtacttg gttgatatac tctttttcgt
     4081 tgcttaaatc atcatctggg ctaagtgcga gggctaattt atatttggca accggcattt
     4141 ccaatagcat aaaaatgacg actaagaaaa tcacaaaaat attcgataat gcaccggata
     4201 gccccattag cagtttgctg accaaattca ttattgagct tgggtcaaat ttgctcataa
     4261 tctcttcttg tgagagggta agtggaatat gccatctatg ggcgagttct agaatcacct
     4321 ggattctaga tgaaagctga attttgtatt gcggaatagc attagtaaat tcttgcaagg
     4381 tgttattaat aatgcctgct aagaaactga atcctaagat aaaaacaata agtaaaatgg
     4441 taatggctaa ccataatggc actttacgct tagtcataaa tttgatcatt ggtgaacaaa
     4501 taatggcaat aaatagcgaa agtaaaaatg gcacaacaat ttcattggat aatttaatcc
     4561 ctgccagaat aatgataatg gaggctatcg ccacgagagt tttggtggaa tgtgtcataa
     4621 tttttccttt tacaagcggt taaaaatcag caatttttta caaattagtg attctcaaac
     4681 caactttcta aaattaaaca agcggaaatg gaatccactt tatccttttt taatgctttg
     4741 taaccaccac gagaaaaaat ttccgctttt gcttcaacag tagtgaggcg ttcatcttgt
     4801 agctcaaccg gcagattaaa gcgtccgttt aagcgattgg caaattttct ggcacgctga
     4861 gttaaaggct gctctgtgcc gtccatattc aagggtagcc caacaaccag catatctggt
     4921 ttccaatctt gcaagagttt tccgatctct tcccagtttg gaataccgtc ttgggcttta
     4981 aatgtagtta aaccttgagc tgtgccggta atactttgtc caactgcaca gccgatgcta
     5041 tttgtgccaa aatcaaaggc aatcacggtt tttgttgtgg tttctgtcat taagctctcc
     5101 cagctcttga cggtatgctt gaaagcccca tcatttcata ggcttctgcc caacgctcta
     5161 aataaccggt ttcaaacagc gttttttcat tcgcatcaga cacaatccaa taattttgag
     5221 caatttcttg ctcaagctgg ctgccacgcc aagtggaaca acctaaacac acaataaatt
     5281 tttcaggtga agccagtgtg ccaaaggagt ctaacacatc accggaggta gtgagcgtaa
     5341 ttttatcggt gatcggataa gtgtgaataa aatcattggc ggttttggtg tgaataataa
     5401 agccacgatc ttgatttacc ggcccaccgc ttaacaccat ttggtctttt ttgtaatcac
     5461 gctggttcgc catttggaaa tccattcggg taatcagctc tagaaccgac agatcggtag
     5521 gagtgttaat tactactccc attgcacctt gcggattatg ttcgcaaata tagaccaccg
     5581 tacgctcgaa atagtcatcg tccatttccg gcgtggcaat taaaaattta ccttgtaaat
     5641 tctctaacat taaatatctc caaaacaaac ttgtaacgca gtaattgccg caagtgaggc
     5701 ggtttctgtg cgtaacactc gtttacccag taatacttca gtaaaacctt cgctctcggt
     5761 catggcgatt tcttcggcag ataatccgcc ctcagagcca atcagtaaac gaacaccagc
     5821 tttcggcaca ttcggcagtt cacgaatggt atatttggct cttgggtgca aattcagttt
     5881 caacatatcg tcttgttctt tgcaccagtc ggtgagtttc ataatcgggc gaatttcagg
     5941 gatttcattt cgtccgcatt gttcacaggc ggcaatcgca attttctgcc attgctgtaa
     6001 ttttttatct tgccgttcat cattgagttt cacgccacaa cgttccgacc aaagtggggt
     6061 aatagttttt acccccagct ccaccgattt ttgaatggta aattccattc tatcgcctcg
     6121 ggaaatcact tgccctaaat ggatcggcag gttggattct cggtcatcta attcacttga
     6181 atcaattttt gcaataattt gctttttttc gaccgcttgt aacgtagcgt ggaaaacgtg
     6241 gtttgaacca tcaaataaaa tgatttgctc tccttgtttc attcgtagca ctcgcccaac
     6301 gtggttagcg gcatcttcac ttaacacgca actttgaaga ccagctaacg gctgtggttg
     6361 gtaaattctt gggattctca taattatcta gttataaatt aaaggcagac gattgtctgc
     6421 cataggtaag ttaaaacggg ttaaggataa agtcttgaac aatgcacaat agtaataatc
     6481 gaaatttcat tctctttaac ttcacacact attctatagc cgttatgata t
//
LOCUS       DANLYI010000143         6361 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.144, whole genome shotgun sequence.
ACCESSION   DANLYI010000143 DANLYI010000000
VERSION     DANLYI010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 6361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6361
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.144"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            61..2430
                     /gene="malP"
                     /locus_tag="PXD81_001886"
     CDS             61..2430
                     /gene="malP"
                     /locus_tag="PXD81_001886"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HDL5537890.1"
                     /translation="MTFNSIVEKYCRYFDVTDPKHFTLQQWYQVVAEGSLELIYSQPA
                     TKPTESRHVNYLSMEFLIGRLTGNNLMNLGYYEQIRDYLKQYQVELVDVLEQERDPAL
                     GNGGLGRLAACFLDSMATVGQNATGYGLHYQYGLFKQSFKEGMQKEAADTWARDSYPW
                     HRFNPSKTRHIGFGGKIKHIQADQYEWQPKLTIQGKAFDLPIVGYKNNIIQPLRLWQA
                     DSDQSFDFDAFNEGKFLSADKTIVNAAALTQVLYPNDNHQAGQKLRLMQQYFHCACSV
                     ADILDRHISEGYSLEDLAKRQVIQLNDTHPTLAIPELMRVLLDEHQFTWEQAWDICSN
                     TFAYTNHTLLPEALEQWDQRLFKQLLPRHYQIVEKINDIFHDKVQAEFGDDAKIWEKL
                     AVLFDYRVRMANLCVVTCFRVNGVAEIHSDLLVTDLFPEYHKLFPTKFCNVTNGITPR
                     RWIRQANPQLSDLLDRTLKQDWTHNLELLKGVEQYADDTGFREEYQRIKRNNKVVLAN
                     EIHKTLGLNVNPNAIFDVQIKRFHEYKRQHLNLLNIIATYQSLKANPNQDYTPRLFLF
                     SGKAAPGYYLAKNIIHAINNVADIINNDKQVNDRLQVAFLPDYRVSLAEKIIPAADVS
                     EQISMAGKEASGTGNMKLALNGALTLGTLDGANVEIADMVGEENVFIFGHTVESVREL
                     LAKGYQPKAYYNQDPILKNAVDFLATGKASNGDKEAFKLMLDSLLERDPFLVFADFDS
                     YRQAQEKIGVAYLDQETWLRSAILNTARLGAFSSDRSIKDYQQRIWLNR"
     gene            2512..4590
                     /gene="malQ"
                     /locus_tag="PXD81_001887"
     CDS             2512..4590
                     /gene="malQ"
                     /locus_tag="PXD81_001887"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HDL5537891.1"
                     /translation="MKITAKQYYRAGIMPYFFDERGVKKTASEKIKHSLFKAFNGNTQ
                     ARETPIPTVNIFYQHQPCFLPFHLADKKHPLTGTWQLQLENSNQIISGKIKARGITLP
                     QDLPLGYHQLQLKTVDKTFNCTIIIAPERCYQPQAVIEQKRLWGTFLQLYTLKSAHNW
                     GIGDFGDLKDFIQQIAPYQADFIGLNPIHSLFPANPNSASPYSPSSRRWLNIAYIDIN
                     QLAEFQQSDEAQTWFHSAEIQQQLSELRTQDWLNYEQIIPLKLKGLRFAFAKFQQNMT
                     ACAAPAFIEFVQKGGESLQVQATFDALHQHLSAQFADQWGWDFWEKAYQDYHSAAVQQ
                     FRETHKTDIEFYLWLQFVADQQLAECDAICRQQNMRIGMYRDLAVGVSPNGAETWNDK
                     QLYCLNASVGAPPDVLGPQGQNWGLTPMNPHVLKQQAYRPFIELVRANMSHCGALRLD
                     HIMSLLRLWWIPKGDSAVNGAYIRYPVDDLIAILALESQRHQCLIIGEDLGTVPKEIV
                     SKLKNAGILSYKIFYFEFDEQGKSRDLQHYPYQAMTTLSTHDLPTINGYWRGYDFELG
                     EKFGVYPNSNILAILKQDRVNAKAKILQRLQEHNVEIAPEISEQLNSPVNTAFIHCLQ
                     TYVANVSSGLFGFQPEDWLGMTEPVNIPGTSTQYANWRRRLSVNLEEIFTDKDIQALL
                     KEVNLIRKNQ"
     gene            complement(4655..6316)
                     /locus_tag="PXD81_001888"
     CDS             complement(4655..6316)
                     /locus_tag="PXD81_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HDL5537892.1"
                     /translation="MSEIALTVSLLSLVAVLGLWIGHIKIRGVGLGIGGVLFGGLFVS
                     HFMAQFDVKLDAHTLHFIQEFGLILFVYSIGIQVGPGFFASLKHSGLKLNGFAVLIVL
                     ISGILVILIHKFFNVPLPVILGIFSGAVTNTPSLGAGQQVLAELSAESVTEIMGMSYA
                     IAYPFGIIGILLSMWLIRILFRINIEAEAEAFNKQQNRSKDQLSTLNVRLTNPNIHGL
                     TLKQIPDFNLHEVAYSRLKRGNELSIPRVDTILQIGDILHLVGEESSLRKMQIILGEA
                     VDVSVSTKGTIYRSERAVVTNEKVFGKQIRQLMLKGKYDVVISRLNRAGVELVPTGDM
                     TLQFGDVLNLVGRQADIEMVMTIIGNAHSKLQQVQMLPIFIGIGLGVLLGSVPIYLPG
                     FPVALKLGLAGGPLVVALILARIGSFSKLYWFMPPSANLALREIGIVLFLAVVGLKSG
                     GRFLETLLSSEGVEWICYGAIITFVPLMIAGLIARMYNKVNYLTICGLLAGSMTDPPA
                     LAFANEIKEGNGAAALSYATVYPLVMFLRIMLPQVLAIMLWVSGG"
BASE COUNT         2032 a         1377 c         1198 g         1754 t
ORIGIN      
        1 gatttgattt tatttggatt ttacataata gcgacattca tctttaaccg ggggtttcct
       61 atgacattta attctattgt agaaaaatac tgtcgctact ttgatgtgac agatccaaaa
      121 cactttacct tacagcagtg gtatcaagtt gttgctgaag gttcccttga gctaatttat
      181 agccagcctg caaccaaacc gacagaatca cgccacgtga actacctctc aatggagttc
      241 ttaatcggtc ggttaaccgg caataattta atgaatttag gttattacga acaaattcgt
      301 gactatctta aacaatatca agttgaatta gtagatgttt tagaacaaga gcgtgatccg
      361 gcattgggta atggcggatt aggtcgtctt gctgcctgct ttttagattc gatggcaact
      421 gtcggacaaa atgcaaccgg ctatgggctt cattatcaat atggtctgtt taaacaaagc
      481 ttcaaagagg gaatgcagaa agaagcagcg gatacttggg ctcgtgacag ctacccgtgg
      541 catcgtttta atccgtctaa aacccgccat atcgggtttg gtggaaaaat caaacatatc
      601 caagccgatc aatatgaatg gcagcctaaa ttgaccattc aaggcaaagc ctttgacttg
      661 ccgattgtag gttataaaaa caacatcatt caaccattac gtttatggca agcagatagt
      721 gaccaatctt ttgattttga tgcctttaat gagggtaaat ttttaagtgc cgataaaacg
      781 attgtcaatg cagccgcact aactcaagtg ctgtatccaa acgacaacca tcaagccgga
      841 caaaaattac gcttaatgca gcaatacttc cattgtgcct gctcggttgc cgatatttta
      901 gatcgtcaca ttagcgaagg ttattcgtta gaagatttag caaaacgcca agttattcaa
      961 ctcaatgata cccatccaac gctcgctata cctgaattga tgcgtgtact gttagatgaa
     1021 catcaattca cttgggagca ggcttgggat atttgcagca atacctttgc ttacactaac
     1081 cataccttat tacctgaagc acttgaacaa tgggatcaac gcctatttaa gcagctatta
     1141 cctcgccatt atcaaattgt ggaaaaaatc aatgatattt tccacgacaa agtacaggca
     1201 gaatttggtg atgatgcaaa aatttgggaa aaattagccg tgctatttga ttaccgtgtg
     1261 cgtatggcaa acctctgcgt tgtcacctgc ttccgtgtaa acggcgttgc ggaaattcac
     1321 tccgatctct tagtgaccga tctattcccg gaataccaca aattattccc aacaaaattc
     1381 tgcaatgtta ctaacggcat cacacctcgc cgctggattc gacaagctaa tccacaatta
     1441 agtgatttat tggatcgtac cttaaaacaa gattggacac acaatctgga attattaaaa
     1501 ggcgttgaac aatatgcgga tgacaccggt ttccgtgaag aatatcaacg cattaaacgc
     1561 aataacaaag tggttttagc caatgaaatc cataaaacat tagggttaaa tgtcaatcct
     1621 aatgcgattt ttgatgtgca aattaagcgt ttccacgaat ataaacgcca acatttgaat
     1681 ttgttaaaca ttattgcgac ctatcaatcg ctaaaagcta acccgaacca agactacaca
     1741 ccacgtctat tcctattctc gggcaaagct gcaccgggtt attatttagc aaaaaatatt
     1801 attcacgcta ttaataatgt ggcagatatt attaataacg acaagcaggt aaatgatcga
     1861 ctacaagtgg cattcttacc ggattatcga gtcagtcttg cggaaaaaat tattccagca
     1921 gctgatgtat cagaacagat ttcaatggca ggtaaagaag cctccggtac cggtaatatg
     1981 aaattggcac tcaacggcgc attaacctta ggtacgctag acggtgccaa tgttgaaatt
     2041 gctgatatgg tcggtgagga gaatgtcttt atcttcggtc acaccgtaga aagcgtgagg
     2101 gaattattag caaaaggcta ccaaccgaaa gcctactata accaagatcc gattctgaaa
     2161 aatgcggttg atttcttagc cacaggaaaa gcctctaacg gagataaaga ggcattcaag
     2221 ctgatgttag atagcctgtt ggaacgagat cctttcttag tctttgccga ctttgatagc
     2281 tatcgccaag cacaagagaa aattggtgta gcctatttag atcaagaaac ttggctgcgt
     2341 agtgcaattt taaatactgc tcgtttgggt gcatttagct cagaccgctc aattaaagat
     2401 tatcaacaac gtatttggtt gaatagataa taaattcggg cgacttctac aataaaggaa
     2461 gtcgcctatt ttctgctgta tttcatatgt atttatatat ggagagaaat gatgaaaata
     2521 actgccaaac aatattatcg ggctggaatt atgccctatt ttttcgatga gcgtggtgta
     2581 aaaaaaacag catctgaaaa aataaaacat tcgttattca aagcctttaa cggcaatact
     2641 caagcaagag aaacacctat ccctacggtc aacatttttt accagcacca accctgtttt
     2701 ttaccttttc atttagcgga taaaaaacat ccgttaaccg gtacttggca gctacaacta
     2761 gaaaattcaa accaaattat ttccggcaaa attaaagcaa ggggaattac attacctcaa
     2821 gatttgccct taggttatca ccaattacag ctaaaaacgg tagataaaac ctttaattgc
     2881 accattatta tcgctccgga acgctgctac caaccacaag ccgtcattga acaaaaaaga
     2941 ttgtggggaa ccttcctaca actatacacc ttaaaatcag cccacaactg ggggattggc
     3001 gattttggcg atttaaaaga cttcattcaa cagattgccc cttaccaagc agattttatc
     3061 ggcttgaacc cgattcactc actttttccg gcaaacccga attctgccag cccatacagt
     3121 ccatcctctc gccgatggct gaatattgct tacattgata tcaatcagct agcggaattt
     3181 caacaaagtg atgaagcaca aacttggttt cattctgctg aaatccaaca acaattatca
     3241 gaattacgca ctcaggattg gttgaattac gagcaaatca ttccgttgaa attgaaaggg
     3301 ctacgctttg ctttcgcaaa atttcagcaa aatatgaccg cttgcgctgc ccctgctttt
     3361 atcgaatttg tacaaaaagg tggtgaaagc ttacaagttc aagccacatt tgatgccctt
     3421 catcagcatc tatcggcaca atttgccgac caatggggct gggatttctg ggagaaagcc
     3481 taccaagact accactccgc agccgtgcag caatttagag aaacgcataa aaccgacata
     3541 gaattttacc tttggttaca attcgttgcc gatcaacagc tagcagaatg tgatgctatc
     3601 tgccgtcagc aaaatatgcg tattggtatg tatcgcgatc tcgccgttgg cgtcagcccg
     3661 aatggtgcag aaacgtggaa tgacaaacaa ctttattgct taaatgcctc tgtcggcgct
     3721 ccgcctgatg tattaggtcc gcaaggacaa aactggggct taaccccaat gaatccgcat
     3781 gtattaaagc aacaagccta ccgtccgttt attgagttag tcagagctaa tatgagtcat
     3841 tgcggtgcat tacgccttga ccatattatg tctttactcc gtttatggtg gatccccaaa
     3901 ggcgatagtg cggttaatgg tgcttatatt cgttatccag ttgatgattt aatcgcaatt
     3961 ttggcacttg aaagccaacg ccaccaatgc ctaattatcg gtgaagattt aggtactgtg
     4021 ccaaaagaaa ttgtgagcaa gctcaaaaat gcggggattt tatcttacaa aattttctat
     4081 tttgaatttg atgaacaagg gaagagtcga gacttacagc attaccctta ccaagcaatg
     4141 accacattaa gcacccacga tttaccgact atcaatggtt attggcgtgg ctatgatttt
     4201 gaattaggcg aaaaattcgg cgtctatcct aattcgaata ttttggcaat ccttaagcaa
     4261 gatcgagtca atgccaaagc caaaatctta caacgcttac aagaacataa cgtagagatc
     4321 gcacctgaaa ttagcgagca acttaattct ccggtgaata ccgcatttat tcactgttta
     4381 caaacctatg ttgcaaatgt aagcagcgga ttattcggct tccaacccga agactggttg
     4441 ggtatgactg agccggttaa cattccggga accagtacac aatatgcaaa ctggcgacgt
     4501 cggcttagtg ttaacctcga ggagatcttc acagataaag atattcaagc cttattaaaa
     4561 gaagttaatt taatccgtaa aaatcaataa aagttagata agaaaaaagg cgactttccg
     4621 tcgccttttg ggcttatatg cttgatgtct atctttatcc acctgaaacc cacaacataa
     4681 tcgctagcac ttgaggtagc ataatacgca aaaacattac tagtgggtaa acagttgcat
     4741 aggataacgc tgcagcgcca tttccctctt taatttcatt ggcaaaagca agcgctggag
     4801 gatcagtcat tgagccggca agtaatccac aaatcgttaa atagtttact ttgttataca
     4861 ttctggcaat caatcctgct atcatcaatg gaacaaaagt aataatcgcc ccatagcata
     4921 tccactcaac gccctcactg cttaatagcg tttcgagaaa tctaccgcca gattttaaac
     4981 caactaccgc taaaaacagt acaataccga tttcacgcaa tgcaagattg gcacttggtg
     5041 gcataaacca gtagagttta ctgaaactac caattcttgc aagaataaga gcaaccacca
     5101 gtggtccacc agccaagccc agctttaacg caacagggaa acccggtaaa taaatcggta
     5161 cagaaccaag taatactccc aaaccgatcc cgataaatat tggcaacatt tggacttgtt
     5221 gcaactttga gtgcgcattg ccgataatcg tcattaccat ttcaatatca gcttgccgcc
     5281 ctactaaatt gagaacatca ccaaattgta acgtcatatc acctgttggt actaattcta
     5341 caccggctcg atttaaacga gaaatgacaa catcgtattt gcctttaagc attagctgac
     5401 gaatttgttt cccgaagact ttttcattcg tcactacagc acgttcagaa cggtaaatcg
     5461 tgcctttggt tgagacagat acatctactg cctcacctaa aataatctgc atttttcgta
     5521 atgaggattc ttcacctact aagtgtaaaa tatctcctat ctgtagaatc gtatctacac
     5581 gtggaataga aagctcattc ccccgtttta agcgtgaata agctacttcg tgcaaattaa
     5641 aatcaggaat ttgtttcaac gttaaaccgt gaatatttgg attagtcaga cggacattta
     5701 aggtactaag ttgatcttta cttctatttt gctgtttgtt aaaagcctct gcttccgcct
     5761 caatattgat tcgaaataaa atccgaatca gccacattga aagtagaatt ccgataatgc
     5821 cgaacgggta agcaatcgca taactcattc ccataatttc agttacactc tcagcgctta
     5881 actcagccaa tacctgttga cctgctccta aagaaggggt attggttaca gccccggaga
     5941 aaatgcctaa aatcacaggc aaaggaacat tgaagaattt atgaataaga ataactaaaa
     6001 taccgctaat cagtacaatc agtaccgcaa aaccattgag ttttaagcca gaatgtttta
     6061 gtgaggcaaa gaatccaggt cctacttgaa ttccaattga ataaacaaat aaaatcagcc
     6121 cgaactcttg aataaaatga agcgtatggg catcgagctt cacatcgaat tgcgccataa
     6181 aatgggagac aaacagccca ccaaataaca ctccccctat acctagcccc acgcctctga
     6241 ttttaatatg gccaatccat aagcctagaa cggccactaa agataataaa ctgacagtaa
     6301 gtgcaatttc gctcattaca gcctctcttt aatatgaaat acctcaaaaa tggtatccaa
     6361 t
//
LOCUS       DANLYI010000144         6275 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.145, whole genome shotgun sequence.
ACCESSION   DANLYI010000144 DANLYI010000000
VERSION     DANLYI010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 6275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6275
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.145"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            42..596
                     /gene="srlA"
                     /locus_tag="PXD81_001889"
     CDS             42..596
                     /gene="srlA"
                     /locus_tag="PXD81_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005725059.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HDL5537893.1"
                     /translation="MIEAITHGAEWFIGLFQKGGETLVGMINGILPLLISLLVVMNAL
                     IKFVGQDRIERLAQRSAGNPISRYLILPVIGTFVFCNPMTLSLGRFLPERYKPSYYAA
                     ASYSCHSMNGLFPHINPGELFVYLGIASGLTTLGLPLGPLAISYLLVGMFTNFFRGWI
                     TDFTTAFFEKRMGITLDREVHLSH"
     gene            627..1628
                     /locus_tag="PXD81_001890"
     CDS             627..1628
                     /locus_tag="PXD81_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HDL5537894.1"
                     /translation="MSNKAIYIEKGNGGWGGPLTLPVVEGKKVLYMTGGTRPAIVNKL
                     VELTGWEAVDGFKEGEPPAEEIGIAIIDCGGVLRCGLYPKRRIPTINIHSTGKSGPLA
                     EFIVEDIYVSAVKENNIRLIDADGSVPVATEQAKPTSSTTATAKEYDSSKKITEQSDG
                     LLAKIGMGMGSVAALFFQAGRDTIDTVLKTILPFMAFVSALIGIIIASGLGDVIAHGL
                     TPLANSPIGLVTLALICSFPLLSPFLGPGAVIAQVIGVLVGTQIGLGNIPPHLALPAL
                     FAINAQAACDFIPVGLSMAEAKQDTVRVGVPSVLVSRFLTGAPTVLIAWAVSAFIYQ"
     gene            1673..2071
                     /gene="srlB"
                     /locus_tag="PXD81_001891"
                     /gene_synonym="gutB"
     CDS             1673..2071
                     /gene="srlB"
                     /locus_tag="PXD81_001891"
                     /gene_synonym="gutB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HDL5537895.1"
                     /translation="MHRLSKRENIYMTIIYQTTFTKIGNFAQESLTDDMLITFKQGAP
                     ADLQDYCFIHNPSELSSPLEVGDIAEFDGVAYPITAVGSVASENLSALGHITFRFDGA
                     NDAEFPGSVHVIGTPPQGLTENSTLIIKRD"
     gene            2119..2907
                     /gene="srlD"
                     /locus_tag="PXD81_001892"
     CDS             2119..2907
                     /gene="srlD"
                     /locus_tag="PXD81_001892"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HDL5537896.1"
                     /translation="MNNEKKVAIVVGGGQTLGAFLSEGLADAGYRVVVADLNGENAQK
                     VAADINAKQGEGNAIGVQVDAADEPSVEALAKATDTVFGRADVLVYSAGTAKASPIWD
                     FNLNDFDLSVKVNLTGYFLTAKHFSRLMIRDGIKGRIIQINSKSGKVGSKYNSGYSAA
                     KFGGVGLTQSLALDLADKGITVHSLMLGNLLKSPMFQSLIPQYAAKLGIPESEVEQVY
                     IDKVPLKRGCDYQDVLNVLKFYAGEEAAYCTGQSINITGGQVMF"
     gene            3076..3456
                     /gene="gutM"
                     /locus_tag="PXD81_001893"
     CDS             3076..3456
                     /gene="gutM"
                     /locus_tag="PXD81_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HDL5537897.1"
                     /translation="MDSITLLIILAMIMWLLQAVLGWLQIKAFNQAFMEISKKGKVTV
                     GRNSGRFNPKSVIVLAFDDEKKVVDSLCMKGFSVFARPQKLASVIGLSLDQIVPEKIF
                     PSDKRSQFALKVAISSGYSCSDQK"
     gene            3519..4166
                     /gene="hxpB"
                     /locus_tag="PXD81_001894"
     CDS             3519..4166
                     /gene="hxpB"
                     /locus_tag="PXD81_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HDL5537898.1"
                     /translation="MQINSVIFDMDGVMIDSEPQWAEAQIAVLADLGINITEQDCEQL
                     TRGKRIDELSDTWIKKFQLSVSNQALSEAILAHGCAAIEQHGKALTGLYELLDFLKSN
                     QIKLAVATSSPPVVIKAVFDRLKLWDYFPIRCSAMDEEYGKPHPAVYLSAVKKLGIEK
                     QECIVIEDSVTGLIAAKAANLTTFLVNTDYQNEKFSIADKRMASLLDVIEKLKQH"
     gene            4209..5006
                     /locus_tag="PXD81_001895"
     CDS             4209..5006
                     /locus_tag="PXD81_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005725056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor"
                     /protein_id="HDL5537899.1"
                     /translation="MKPFERRQQILAYLAQHGRTEVDVLADYFKLTGATIRKDLTSLE
                     EDKKVLRTYGSVVLIDEEILEREEKINKEDKDDKPLAQKTGINLQQKQAIGQKAAELI
                     NEGNSIIFDAGSTVLQMIPHLKAFDNLSLMTNSLTIFNEILKLNKSYNLLISGGTFRE
                     KSASFHGYFAESAFIGSNFDILFIGTDGLDLDVGLTTFNEVYKVSSLMCDAANKIVLL
                     ADSSKFGRKSPNIVCGLEKIHTIITDEGLSQEMREQIKAKGIELLVV"
     gene            5017..5952
                     /locus_tag="PXD81_001896"
     CDS             5017..5952
                     /locus_tag="PXD81_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HDL5537900.1"
                     /translation="MNHLDSAKETLNLYAQQIITLNHRLSEEFNEVVNMILACQGRVV
                     VGGIGKSGLVGKKMVATFASTGTPSFFLHPTEAFHGDLGMLKPIDMVILISNSGETDD
                     VNKLIPSLKNFGNKIIAMTGNPYSTLGRNADVILNIGVEREACLNNLAPTSSTLVTMA
                     LGDALAIALMKARDFRPEDFARYHPGGSLGRKLLNRVRDVMVRKVPTASLDTTFTECL
                     SVMNEGRMGVAVIMQDDKLEGIITDGDIRRTLAKFGAESLNKTADEIMTRNPKTINDT
                     EFLAKAEDLMKELKIHSLIAVNDEGKVTGLMEFSS"
     gene            complement(5996..6232)
                     /locus_tag="PXD81_001897"
     CDS             complement(5996..6232)
                     /locus_tag="PXD81_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL5537901.1"
                     /translation="MFTRAAKQRNNVKQYQLWQHHNQPITIYSQKFFDEKLNHIHNNP
                     IVSGFVCEAFEWKYSSARNYANNLPVLLDIDICQ"
BASE COUNT         1885 a         1154 c         1408 g         1828 t
ORIGIN      
        1 gatgaaagaa aaatatttta ttttgaaaaa cggagatcgg tatgattgaa gcaattacac
       61 acggagcaga atggtttatc ggcctgttcc aaaaaggcgg tgaaacctta gtcggtatga
      121 taaacggtat tttgccactc ttaatttcat tattggttgt aatgaatgcc ctgattaaat
      181 ttgtagggca agatcggatt gaacgacttg ctcaacgtag tgccggaaac cctatttcac
      241 gttatttgat cttaccggtg atcggcactt tcgttttctg taacccgatg acattaagct
      301 tagggcgttt cttaccggag cgttacaaac caagctacta cgccgcagcc tcttatagtt
      361 gccactctat gaacggctta ttccctcaca tcaacccggg tgaattattc gtttatttag
      421 gtattgccag cggtttaacc accttaggct taccacttgg gcctttagcc atcagttatt
      481 tattagtcgg tatgtttacc aatttcttcc gtggctggat aacagacttc accaccgcat
      541 tctttgaaaa acgaatgggg atcaccttag atagagaagt acatttatcc cactaaccaa
      601 gtaactgaaa tacagaggaa tagattatga gcaacaaagc gatttacatt gaaaaaggca
      661 acggtggttg gggtggtcca ttaacattac ctgttgtgga aggcaaaaaa gtgctttata
      721 tgaccggcgg cactcgccct gcgattgtta ataaattggt ggaattaacc ggctgggaag
      781 cggttgatgg ttttaaagaa ggcgaaccgc cggcagaaga aatcgggatt gcgattattg
      841 attgcggtgg ggtattacgc tgtgggcttt acccaaaacg ccgtattcca accattaaca
      901 ttcactccac cggtaaatcc ggccctttag cagaatttat tgtggaagat atttatgttt
      961 cagcggtgaa agaaaacaat attcgcttaa ttgatgccga tggaagtgtg cctgtggcaa
     1021 cagagcaagc aaaaccaact tcatcaacga cagcaacggc aaaagagtat gactccagca
     1081 aaaaaatcac cgaacaaagt gatggtttat tagcaaaaat cggtatggga atgggatcgg
     1141 tggcggcgtt attcttccaa gccggacgag acactattga tacggtgctt aaaaccattt
     1201 taccgtttat ggcatttgtt tctgcgttaa tcgggatcat cattgcgtca ggtttaggtg
     1261 atgtgattgc ccacggcttg acccctctgg caaacagccc aatcggttta gttacactgg
     1321 cgttgatttg ttccttccct ctcctttccc cattcttagg gccgggagcc gtgattgcac
     1381 aagttatcgg cgtgttagtc ggtacgcaaa tcggtttagg caatattccg ccacatttag
     1441 cactgccggc actctttgct attaatgctc aagccgcttg tgattttatt cctgtgggtc
     1501 tttcaatggc ggaagcaaaa caagataccg tacgggtggg tgtaccgtca gtattagtca
     1561 gccgtttttt aaccggtgca ccgaccgtgc tgattgcgtg ggcagtttcc gcattcatct
     1621 accaataatt aaccacgata caagcggtgg aatttgaaaa attttttgca aaatgcaccg
     1681 cttgtcaaaa cgagagaaca tttatatgac gattatttat caaacgacct ttaccaaaat
     1741 cgggaatttt gctcaggaat cattaacaga cgatatgtta atcactttta aacaaggcgc
     1801 accggcagat ttacaagatt actgcttcat tcataatcca tctgaattaa gtagcccttt
     1861 agaagtaggc gatatcgctg aatttgatgg cgtagcttat ccgattacgg cagttggtag
     1921 cgttgcatct gaaaacttat ccgccctcgg acacattacc ttccgttttg acggggcaaa
     1981 tgatgcggaa tttccgggaa gcgttcacgt tatcggaaca ccgccgcaag gcttaacaga
     2041 gaactcaacc ttgattatca agcgtgatta atgacggatt aaaaataatt tcaaattgaa
     2101 aatttagagg aatacattat gaacaatgaa aaaaaagttg ctatcgttgt tggcggcggt
     2161 caaacacttg gagctttttt atctgaaggc ttggcagatg ccggttatcg tgtggtggtt
     2221 gctgacttaa acggcgaaaa tgcacaaaaa gtggcagccg acattaatgc caaacaaggc
     2281 gaaggaaatg cgattggggt gcaagtcgat gcggctgatg aaccaagcgt tgaagcacta
     2341 gctaaagcaa cagataccgt attcgggcgt gccgatgtat tggtttacag tgcaggcaca
     2401 gcgaaagcat cgcctatttg ggatttcaac ttaaacgatt ttgatttgtc ggtaaaagtg
     2461 aatttaaccg gttatttctt aaccgcaaaa cacttctccc gcttaatgat tcgtgacggt
     2521 attaaagggc gtatcattca aattaattct aaatcgggta aagtagggag taagtataat
     2581 tccggttata gtgcagcgaa atttggtggt gttggtttaa cacaatcgtt agcattagac
     2641 ttggcagata aaggcattac cgtgcattca ttaatgttag gcaatttatt gaaatcgccg
     2701 atgttccaat cgttaattcc gcaatatgcg gcaaaactcg gcattccgga aagcgaagta
     2761 gagcaagttt atattgataa agtgccttta aaacgtggct gtgattatca agatgtgttg
     2821 aatgtcttaa aattctatgc cggcgaagag gcagcttatt gcacaggaca gtctattaat
     2881 attaccggtg gtcaggtaat gttctaagca aacaagcggt caaatttgtc gattttttta
     2941 caacaatttg caaaaaatga ctaaaataaa ccgcttgtta attcgccaca ccggttaata
     3001 tgccacaggg gaaagcgtat tgttttgaaa tcgcaggtac gtttaggctc tgtggctttt
     3061 tattaaggag caatgatgga ttcaattaca cttctgatta tattggcaat gataatgtgg
     3121 ctgttacaag ccgttttggg ttggcttcaa attaaagcct ttaaccaagc ctttatggaa
     3181 atcagtaaga aaggcaaagt aacggttggg cgtaatagtg gtcgttttaa tcctaaatcg
     3241 gttattgtgt tggcgtttga tgatgagaaa aaagtggtag atagcctttg tatgaaagga
     3301 ttttccgtct ttgccagacc gcagaaatta gcgtctgtga ttggtttgtc tcttgatcaa
     3361 attgtgccgg aaaaaatctt tccaagcgat aaacgttcac aatttgcctt aaaagtggcg
     3421 atatcttccg gatatagttg ctcagatcaa aaataatgcg attttttatt tcattttgaa
     3481 tattttttta gttaaaaatg agataatgga gaataaatat gcagatcaat agtgtcatct
     3541 ttgatatgga cggggtaatg attgattcag aaccccaatg ggcagaggct caaattgcag
     3601 tattagccga cttaggtatt aatatcaccg agcaagattg cgaacaactc accagaggta
     3661 agcggattga tgagttgagc gacacttgga ttaaaaaatt tcagttatct gtttccaacc
     3721 aagcattaag tgaagccatt ttggcacacg gttgtgctgc gattgaacaa cacggcaaag
     3781 cattaacggg tttatatgaa ttattggatt tcttaaaatc taatcagatc aagttagccg
     3841 ttgcaacctc atcgccgccg gtggtgatta aagcggtgtt tgatcgctta aaactgtggg
     3901 attatttccc gattcgatgc agtgcaatgg acgaagaata tggcaagcca caccctgcgg
     3961 tctatttgag tgcagtgaaa aaattaggca ttgaaaaaca agagtgtatt gttattgagg
     4021 acagtgtaac cggcttaatt gccgccaaag ccgctaattt aactacattt ttggtgaata
     4081 ccgattatca aaatgaaaaa ttttcaattg ccgataaaag aatggcatcg ctactagatg
     4141 tcattgaaaa attaaaacag cattagcaca gattagctcg ctccaatctt tgctaaattg
     4201 gaggaacaat gaaacctttt gaaagaagac agcaaatttt agcctattta gctcagcacg
     4261 gtagaaccga agtggacgtg ctggcagact attttaagct aactggtgca accattcgta
     4321 aagatttaac ttcacttgaa gaagataaga aagtcttgcg tacttatggc agtgtagtct
     4381 taattgatga agaaattctt gaacgggaag agaaaatcaa taaagaggac aaagacgata
     4441 aaccgcttgc acagaaaacc ggcattaatc ttcaacaaaa gcaagctatc gggcaaaaag
     4501 ctgcagagtt aattaatgag ggaaactcta ttatttttga tgcaggcagc acagtgttac
     4561 aaatgattcc ccatttaaaa gcatttgata acctctcttt aatgacaaat agtttaacta
     4621 ttttcaatga gattttgaaa ctcaataaat cctataactt actgatttcc ggtggtacat
     4681 ttcgtgagaa atcagcttca tttcacggtt attttgctga atccgctttt attggttcaa
     4741 attttgacat actctttatt ggcacagacg gactagattt agacgttgga ttaacgactt
     4801 tcaatgaagt atataaagtc agttcgctaa tgtgtgatgc ggcaaataaa atcgtcttac
     4861 ttgccgactc cagtaagttt ggacgtaaaa gtccgaatat tgtgtgtggg ttagaaaaaa
     4921 ttcatactat cattaccgat gaaggattat ctcaagaaat gagagaacag attaaagcga
     4981 aagggattga gctgttagtt gtataagggc gtaaaaatga atcatttaga cagtgcaaaa
     5041 gaaacgttaa acctctatgc tcagcagatt atcacgctaa accatcgact ttctgaagaa
     5101 tttaatgagg tggtaaatat gatactcgct tgtcaagggc gagtggttgt tggcggtatt
     5161 ggaaaatcgg ggctggttgg caaaaaaatg gtggcaactt ttgcctctac cggtacaccg
     5221 agctttttct tgcacccgac agaggctttt cacggcgatt tgggaatgct gaaaccgatt
     5281 gatatggtaa ttctgatttc taacagtggt gaaactgatg atgtgaataa actgattcca
     5341 agcctgaaaa atttcggcaa taaaattatt gcgatgacag gtaatcctta ctccacactt
     5401 gggcgaaatg ccgatgtgat tttaaatatt ggtgtagagc gtgaagcctg cctgaataat
     5461 ctagctccaa ccagctctac tttggtaacg atggctttag gcgatgcgtt agccattgcg
     5521 ctaatgaaag cccgtgattt ccgccctgaa gattttgccc gctaccaccc gggtggcagt
     5581 cttggtcgta aattattgaa cagagtgcga gatgtgatgg tgcgtaaagt gccaaccgcc
     5641 agcttagaca ccacctttac cgaatgttta tctgtgatga acgaagggcg tatgggggtg
     5701 gcggtaatta tgcaagacga taaattggaa ggcattatta ccgatggtga tatccgccgc
     5761 acattagcga aattcggggc ggagagccta aataaaaccg ctgacgaaat tatgacccgt
     5821 aatccaaaaa ccattaacga taccgaattt ttagccaaag ccgaagattt aatgaaagag
     5881 ctgaaaattc actcattaat tgcagtgaat gatgagggta aagtaacggg attgatggag
     5941 ttctctagtt aatttataga tgtaacgatg gcgcaagcgt ttacgcttgt gccgttcact
     6001 gacatatatc aatatctaat aaaacaggta agttgttagc ataatttctt gcactgctat
     6061 atttccattc aaatgcctcg catacaaaac cgcttacgat agggttgttg tgaatatggt
     6121 ttagcttttc atcaaaaaat ttctgagaat agattgttat cggttggtta tgatgttgcc
     6181 aaagttggta ttgcttgaca ttatttcgtt gtttagctgc ccgagtaaac atccataata
     6241 accattcttt tctactttct tggggatttg tttta
//
LOCUS       DANLYI010000145         6225 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.146, whole genome shotgun sequence.
ACCESSION   DANLYI010000145 DANLYI010000000
VERSION     DANLYI010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 6225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6225
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.146"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            253..465
                     /locus_tag="PXD81_001898"
     CDS             253..465
                     /locus_tag="PXD81_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDL5537902.1"
                     /translation="MSAITGTVKWFNATKGFGFITPDQGGKDVFVHFSAIEANGGYRT
                     LEDGAKVQFEIQDSERGASAVNVKTI"
     gene            complement(525..1679)
                     /locus_tag="PXD81_001899"
     CDS             complement(525..1679)
                     /locus_tag="PXD81_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HDL5537903.1"
                     /translation="MMKVSPFQWAAFNYFGFFCAYGVLLPFLPVWLKHHGYSTEMIGL
                     FAAVGYLFRFAGSMLASQRVKTVSQLIPTARSLTWLNIAAAAALLFSGDFIWLLFPVL
                     MLFQMFNSGAMPIADSIASIWQHQIGLDYGRARLFGSMAFVVGSLSAGYLIGLLGENS
                     VVWIIIGFLVFLGIGQLATPKVGFEEQGNKANTSSVTYRQLLKEPETLKMLIAVSLIS
                     ASHAAYYTYSTIHWSNASISTAMTSLFWGLAVVAEILFFLVSKRLFKSWKISHLMILA
                     AVFAVIRWAIMGSTTEIVLIIVMQMLHSITFGAAHIAMIRYISMQSAERVTKLQGLYF
                     GLASCAFMAVFTFIAGMVYQHSPSLTFWLMAIFTLPAIFIVPKRFEVQIN"
     gene            complement(1676..2494)
                     /locus_tag="PXD81_001900"
     CDS             complement(1676..2494)
                     /locus_tag="PXD81_001900"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009875061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HDL5537904.1"
                     /translation="MNEKKLAFIGTGNMAYAIIQGLLKSGYSAEQIIACNKSNLARRE
                     ELQAVGIATNFANREAVEQADVIVLAVKPQMMAQVCAEFADVDFSNKWVISVAAGISV
                     QRLETLLPSAKNIIRTMPNTPSLISEGMTGLFAKKSVNPTACQFAEQLMSAVGKCYWV
                     ETEEQINHIIAITGSSPAYFFRFMEAMQQSAVQMGFSEQDARTLVQSVALGAAKLVEA
                     NPTTPLSTLRENVTSKGGTTAKALEVFEQHDLAKTVDDAMWAAIHRAREMEQSL"
     gene            2571..3479
                     /gene="rdgC"
                     /locus_tag="PXD81_001901"
     CDS             2571..3479
                     /gene="rdgC"
                     /locus_tag="PXD81_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HDL5537905.1"
                     /translation="MVWFKNVMIYKLTGELALESNSLNELQQETRFTPCSQHDMSKFG
                     WATPLTGSDLLHFSQANQFLLISHKEDKLLPANVVKAETEQRIAELEEKEARKLKKTE
                     KQAVKDQVVSALLPRAFSKHQFTALWLDLDNQLVYVDAASSKRAEDTLALLRKTLGSL
                     PVVPISFTLPPAEVMTNWIAKGHTPSWLNLLEEAELKSFDTDSIIRCKRQDLETEEIA
                     RHLQAGKFITKLAIEWEGHFNCVLNEDATLSRLKFADEVREKNDDILKEDVAQRFDAD
                     FLLMTEELKLFTEKMIAEFGGIKSRI"
     gene            3500..4105
                     /locus_tag="PXD81_001902"
     CDS             3500..4105
                     /locus_tag="PXD81_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase family hydrolase"
                     /protein_id="HDL5537906.1"
                     /translation="MLDQTIIDQYDALIFDMDGTLIDTMPSHAKAWEKVGEVLGYPIN
                     PKPMYELSGSTTFVIAREIMQRSNIPEHYFEQVVQLKREFGIEMVLANATLLPAFEII
                     KANVGKKPMAIGTGSHRAMVELLDQKFNLRQYVSVIVDSDDVSNHKPAPETFLKCAEK
                     LGIAPHRCLVFEDADLGVQAALNGNMNVFDVRTNTITHSTR"
     gene            4121..4912
                     /locus_tag="PXD81_001903"
     CDS             4121..4912
                     /locus_tag="PXD81_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HDL5537907.1"
                     /translation="MKTEWIKHLIQQLTTLSKAIENYFTSGSFGVKFYPFLMHPCFRK
                     SCAFLIFIIAMVSSFSGLFYLVAEHITILFSERSLTTYFHHSTLELMIPVGTMIDEKI
                     SELSPLSVVMRSMFVLQAVVFFFIYAIGITHIAHNKLRVIGLLLSLCFAIGCSLVAGI
                     QPSSRGEFGIYNLGASITFLIGNLTLIIAGFDLYHPSMQFFKRFSIGAGVIGAICILI
                     TIFLPTLFSPLIERAGIYTIMLWEILAGFAIIKRLLKLPSFRQQG"
     gene            4916..6058
                     /gene="argE"
                     /locus_tag="PXD81_001904"
     CDS             4916..6058
                     /gene="argE"
                     /locus_tag="PXD81_001904"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016533842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HDL5537908.1"
                     /translation="MKQIPFIQRYAQLIELPTISSLVAEEDLSNRRLIELLATWLADF
                     GFKTEILAVEGSRNKYNLLATYGEGEGGLLLAGHTDTVPFDEGKWRFNPFQLTEKAGK
                     LYGLGTADMKGFFAFVVEVVSQLDLTQIKKPIRILATADEETTMLGARTFAQHSHIRP
                     DCAIIGEPTSLKPIRAHKGHIGESVRITGRSGHSSDPAKGINAIELMHQSIGYLMKMR
                     DELREKYHNPLFQVTHPTMNFGNIHGGDAINRICACCELQFDMRPLPNMAVQDLYAMV
                     NEHLKPMLEQYGDLIEIRHLHDGIPGYECEHSAQIVQVVEKLLGEKCDAVNYCTEAPF
                     IQQLCPTLVLGPGSIEQAHQPDEFLEMKYIEPTKELLTKLIYHFCG"
BASE COUNT         1840 a         1417 c         1312 g         1656 t
ORIGIN      
        1 gcttcgtcca tataaattcc acattacgtt tacaaaagag atagactctt ttttcatttg
       61 aatgttcata aaagtataaa aatgatttgc ttaaaaaatg aataagtggt gtaatggctc
      121 aagctgttcg tgcgttaatt aaagttgaat cttgttagtt attttcctca aggcaagtga
      181 ttgatagata cgtctgtaat atcccgttta gctatctttt atcacttttt gtatttatat
      241 aaggaaaatt ttatgtcagc aattaccggc acagtaaaat ggttcaacgc caccaaaggt
      301 tttggtttta tcacccctga tcaaggtggc aaagatgtat tcgtacactt ctctgctatc
      361 gaagcaaacg gcggctaccg cacattagaa gacggtgcta aagtacaatt tgaaatccaa
      421 gattcagagc gtggagcttc agccgtaaac gtgaaaacaa tctaatttca gcaaattcaa
      481 tgaatttcga taaaaatgcc gacaacttgt cggcattttt gctatcaatt aatctgtact
      541 tcaaaccttt tcggcacaat aaaaatggca ggtagggtaa aaattgccat caaccaaaat
      601 gtcaggcttg gggaatgttg atagaccatt ccggcaataa aggtaaatac tgccataaaa
      661 gcacagcttg ccaagccaaa atagagacct tgtaatttcg tcacacgctc tgccgattgc
      721 atcgaaatat agcgaatcat cgcaatatga gccgccccga aggtaataga atgtagcatt
      781 tgcattacaa taatcagcac gatctctgta gtactaccca ttattgccca acgaatcact
      841 gcaaaaacgg cagctaaaat cattaaatgg ctgattttcc acgatttaaa caagcgttta
      901 gagaccaaaa agaacaaaat ttccgccacc accgccaagc cccaaaacag gctagtcatt
      961 gcggttgaaa tactggcatt tgaccaatga atggtgctgt aagtgtaata agcggcgtgc
     1021 gaggctgaaa ttaacgacac cgcgatcaac atttttaagg tttccggctc tttcaataat
     1081 tggcgataag ttacgcttga agtattggct ttattacctt gttcttcaaa cccgactttg
     1141 ggcgtagcaa gctgccctat tcctaaaaaa accaagaaac cgatgataat ccacaccacc
     1201 gaattttcac caagcaagcc gataagatag ccggcagaaa gtgagcctac cacaaacgcc
     1261 attgagccga ataaccttgc tctgccgtaa tccagcccga tctgatgttg ccaaattgag
     1321 gcaatgctat cggctatcgg cattgccccc gaattgaaca tttgaaacaa cattaacacc
     1381 ggaaaaagta gccaaatgaa atcccctgaa aacagcaacg ccgccgcagc cgcaatattc
     1441 agccaagtta aactgcgggc ggtgggaatg agttggctca cggtttttac ccgttgtgag
     1501 gcaagcatac tgccggcaaa gcggaacaaa tagccaacgg cggcaaataa gccgatcatt
     1561 tcagtcgaat agccgtgatg tttaagccac actggcaaaa acggcaacaa cacgccatag
     1621 gcacaaaaaa agccaaagta attaaatgcc gcccattgga acggggaaac tttcatcata
     1681 acgattgctc catttctctg gctcgatgaa ttgccgccca cattgcatca tcaacggttt
     1741 tagcaagatc gtgctgctca aacacctcaa gggctttcgc cgttgtgcct ccctttgagg
     1801 tcacattttc ccgcaaggtc gaaagtggcg tagtcgggtt cgcttccacc aatttcgccg
     1861 cccccaatgc gacagattgt acaagcgttc ttgcgtcctg ctcggaaaag cccatttgca
     1921 ccgcactttg ctgcattgcc tccataaaac ggaagaaata cgccgggctt gagccggtta
     1981 tcgcaataat gtggttgatt tgctcctcgg tttccaccca gtaacattta ccaacggctg
     2041 acatcaactg ttcggcaaat tggcaagcgg tcggatttac cgattttttt gcaaataatc
     2101 cggtcattcc ttcgctgatt aaagacgggg tgtttggcat cgtgcgaatg atatttttcg
     2161 cactcggcaa cagcgtttcc aagcgttgca cagaaatacc tgctgctact gaaatcaccc
     2221 atttgttgga aaaatcaaca tcggcaaact cggcacacac ttgtgccatc atctgtggtt
     2281 ttaccgccag cacgatcacg tcagcctgct ctaccgcctc acgatttgca aaatttgtcg
     2341 caattccgac cgcttgtaac tcctctcggc gagccaaatt acttttattg cacgcaatga
     2401 tttgttcggc agaataaccg ctttttaata agccttgaat aatcgcatac gccatatttc
     2461 cggtgccgat aaaggcaagt tttttctcgt tcattcgcaa attctctact gtgttgggtt
     2521 gaggtctgtt aaaataacac tattctacac ccgataaagg atatgcaaaa atggtttggt
     2581 tcaaaaatgt gatgatctat aaattaaccg gcgagctggc gttggaaagc aatagcctta
     2641 acgagttaca gcaagaaacc cgctttaccc cttgcagcca gcacgatatg agcaaattcg
     2701 gctgggctac gccacttacc ggctcggatt tactgcattt ctcacaagcg aaccaatttt
     2761 tgctgatttc acacaaagaa gataaattat tacctgccaa tgtggtgaaa gccgaaaccg
     2821 agcaacgcat tgcagaactg gaagaaaaag aggctcgtaa gctgaaaaaa accgaaaaac
     2881 aagccgtgaa agatcaggtc gtttctgcgt tattgcctcg agcattcagc aaacatcaat
     2941 tcaccgctct ttggttggat ttagataatc agttggttta tgtggatgca gcctcgagca
     3001 aacgggcgga agatacgctg gcgttactgc gaaaaacact tggctcgttg ccggttgtgc
     3061 cgatttcctt taccctaccg ccggcagagg taatgacgaa ttggatcgcc aaagggcata
     3121 cgccaagttg gttgaatttg ttggaagagg cagaactgaa atctttcgat accgacagca
     3181 ttattcgctg taaacgccaa gatttagaga cggaagaaat cgcccggcac ttgcaagcgg
     3241 gcaaatttat cacaaaactt gcgattgaat gggaagggca tttcaattgc gtgttaaatg
     3301 aagacgccac gctttcacgc ctgaagtttg ccgatgaagt gcgagagaaa aatgacgata
     3361 ttctcaaaga agatgtggct caacgctttg atgccgattt cttattaatg accgaagagc
     3421 tgaaactttt caccgaaaaa atgattgccg aatttggcgg aattaaatcc agaatttaga
     3481 aaaaaattta aggaaagcaa tgttagatca aaccattatc gaccaatatg acgccttgat
     3541 ttttgatatg gacggcacat taattgacac gatgccaagc cacgccaagg cgtgggaaaa
     3601 ggtgggcgaa gtgttaggct acccaatcaa tccaaagcca atgtacgaac tgagtggctc
     3661 aaccactttt gtgattgccc gagaaataat gcaacgctcc aatatccccg agcattattt
     3721 tgagcaagtg gtacaactaa aacgggaatt tgggattgaa atggtgttag ccaatgccac
     3781 tctgttgcct gcctttgaga ttattaaagc taacgttggc aaaaaaccaa tggcaatcgg
     3841 cacaggctca caccgtgcaa tggtggaatt attagaccaa aaatttaact tacgtcaata
     3901 tgtttcggtt attgtagatt ctgatgatgt aagcaaccat aagccagccc ctgaaacctt
     3961 tttaaaatgt gcggaaaaac ttggcattgc cccacaccgc tgtttggtgt ttgaagatgc
     4021 cgatttaggc gtgcaagccg ccctcaacgg caatatgaac gtgtttgatg tacgtacgaa
     4081 tacgataact cactcaaccc gttaaaaatg ataggtttat atgaaaacag aatggattaa
     4141 acatttaatt caacaattaa ccacgctttc caaagccatc gaaaactact ttaccagcgg
     4201 ttcatttggg gtgaaattct acccttttct aatgcaccct tgttttcgta aaagctgtgc
     4261 ctttttgatt tttattattg ctatggtttc aagttttagc gggctatttt atttggtggc
     4321 ggaacacatc acgattttat ttagcgaaag atcgctcacc acttacttcc accatagcac
     4381 ccttgaattg atgattccag tcggcacaat gatagatgaa aaaatcagcg aattatcacc
     4441 actttcggtg gtaatgcgtt caatgtttgt gctacaagca gtcgtttttt tctttattta
     4501 tgcaatcggc atcacccaca ttgctcacaa caaattgcgg gtcatcggct tattgctttc
     4561 gctatgcttt gcgattggct gctcactagt tgccggtatt cagccaagca gtcgtggcga
     4621 attcggcatt tacaatttag gggcaagcat tacgttttta atcggcaatt taacgctgat
     4681 catcgcaggc ttcgatctct accacccttc aatgcaattt tttaaacgct ttagcattgg
     4741 tgcgggcgtt atcggggcaa tctgtatttt aatcacgatt ttcctaccaa ccctgtttag
     4801 cccgttaatt gaacgagcag gtatttatac cattatgctg tgggaaattt tagccggttt
     4861 tgccatcatt aaacgcctgt tgaaattgcc ctcattcaga caacaaggat aaccaatgaa
     4921 acagattccc tttattcaac gctatgctca actgattgag ctaccgacca tttccagcct
     4981 tgttgccgaa gaagatttat cgaaccgccg tttgattgaa ttgctcgcca cttggctggc
     5041 agatttcggc tttaaaaccg agattttagc ggtagaaggc agccgtaata aatacaattt
     5101 acttgcgacc tatggcgagg gtgagggcgg cttattattg gcagggcata ccgacaccgt
     5161 gccgtttgat gaaggaaaat ggcgcttcaa tccattccaa ttaaccgaaa aagcaggcaa
     5221 actctacggc ttaggcacgg cagatatgaa aggctttttc gcctttgtgg tggaggttgt
     5281 gagccaactc gacctaaccc aaatcaaaaa gccgattcgc attcttgcca ccgctgatga
     5341 agaaaccaca atgctggggg caagaacctt tgctcagcac agccacattc gccccgattg
     5401 tgcgattatt ggcgagccga cctcgctcaa accgattcga gcccacaaag ggcatattgg
     5461 cgaatcggtt cgcattaccg gcagaagcgg acactccagc gaccccgcca aaggcattaa
     5521 tgcgattgaa ttaatgcacc aatctatcgg ttatttaatg aaaatgcgtg atgaactgcg
     5581 agagaaatac cacaatccgc tgttccaagt cacccacccg acaatgaact tcggcaatat
     5641 tcacggcggc gatgccatta accgcatttg tgcctgttgc gaattgcaat ttgatatgcg
     5701 accactaccg aatatggcgg tgcaagattt gtatgcaatg gtaaacgagc atttaaaacc
     5761 gatgctggaa caatatggcg atttaattga aatccgccat ttacacgatg gaattccggg
     5821 ctacgaatgc gaacactcgg cacaaatcgt gcaagtggtt gaaaagctac tgggcgaaaa
     5881 atgcgatgcc gtcaattact gcaccgaagc cccattcatt caacaactct gcccaacgct
     5941 ggtactaggc cccggctcca ttgaacaagc ccaccagccc gatgagtttt tggaaatgaa
     6001 atatatcgaa ccgacaaaag agttattgac taaattgatt tatcattttt gtggataaat
     6061 caacagaaac aagcgctcat tttcgccata ctttttgcaa aagaatcggc aaaaatgacc
     6121 gcttgatatt tagtattgag tgacctataa acttccatac ttcatcattg atttaaggag
     6181 attctgttag ataggtatta atctctagct aggtcttgga tacaa
//
LOCUS       DANLYI010000146         6116 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.147, whole genome shotgun sequence.
ACCESSION   DANLYI010000146 DANLYI010000000
VERSION     DANLYI010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 6116)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6116)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6116
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.147"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            complement(<1..285)
                     /locus_tag="PXD81_001905"
     CDS             complement(<1..285)
                     /locus_tag="PXD81_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5537909.1"
                     /translation="MSTTLNEINITELDAGPEIKSLSLNDAVNSFLNDSACPICGKNC
                     QYWQRLKRSLNRKENQGKGDKVLVKNPSPISIEKNLINAYKEYRKFEEDLK"
     gene            complement(319..1605)
                     /gene="purD"
                     /locus_tag="PXD81_001906"
     CDS             complement(319..1605)
                     /gene="purD"
                     /locus_tag="PXD81_001906"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012771675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HDL5537910.1"
                     /translation="MNILIIGNGGREHALAWKVRQSPLADKVFVAPGNAGTAMENGIE
                     NVAISATDVPALVTFAKQNSVGLTIVGPEAPLVVGVVDAFRAHGLTIFGPTQAAAQLE
                     GSKAFTKDFLARHQIPTAEYQNFTEIEPALAYLKQKGAPIVIKADGLAAGKGVIVAMT
                     LQEAEDAVRDMLSGNAFGEAGSRVVIEEFLDGEEASFIVMVDGKNVEPMATSQDHKRV
                     GEGDSGLNTGGMGAYSPAPVVTKAIHQRVMDEIIYPTVQGMASENTPYTGFLYAGLMI
                     MPNGQPKVIEFNCRFGDPETQPIMMRLESDLVELCLKACEGKLDSVQSKWCEQAALGI
                     VLAAAGYPGDYRKGDEIIGIPTSTSNQKVFLAGVEQKEGKLLTNGGRVLCATALGNSV
                     FEAQQQALQLAEQIQWQGKFYRRDIGYRAVARKQAK"
     gene            1839..2213
                     /gene="rpsF"
                     /locus_tag="PXD81_001907"
     CDS             1839..2213
                     /gene="rpsF"
                     /locus_tag="PXD81_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011199651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HDL5537911.1"
                     /translation="MRHYEIVFMVHPDQSEQVPAMIERYTASVKEAGGQVHRLEDWGR
                     RQLAYPINKLHKAHYVLMNVEAPQSVIDELETNFRYNDAVLRNLIVHTKAAVTEASPM
                     VKAKESKVAEAVAEVESEEAGE"
     gene            2188..2562
                     /gene="priB"
                     /locus_tag="PXD81_001908"
     CDS             2188..2562
                     /gene="priB"
                     /locus_tag="PXD81_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005598106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HDL5537912.1"
                     /translation="MNQRKQASNLPIDNCLILSGTVSSLVKQSLNPLGVPHYSFWLEH
                     RSIQTEVNLERQAWCKIQVVLAGNQFSYLTQQIKLGESVRIKGFIHTHKDFNGLNQLV
                     LHTEQIEFIDQEKPNGTLFPSS"
     gene            2537..2764
                     /gene="rpsR"
                     /locus_tag="PXD81_001909"
     CDS             2537..2764
                     /gene="rpsR"
                     /locus_tag="PXD81_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HDL5537913.1"
                     /translation="MARYFRRRKFCRFTAENVVEIDYKDIATLKNYISESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLALLPYTDNHQ"
     gene            2780..3229
                     /gene="rplI"
                     /locus_tag="PXD81_001910"
     CDS             2780..3229
                     /gene="rplI"
                     /locus_tag="PXD81_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011609589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HDL5537914.1"
                     /translation="MQVILLDKVAHLGSVGDQVTVKSGFARNFLIPQGKAVMATAANI
                     AHFEARRAELEAKAAAALATAQERAAKIAALAAVSVSATAGDDGRLFGSVSAKDIAEA
                     LTAAGVGVAKSEVRLGEGPLRTTGEHQVKIHLHPEVNATVAVNVVAE"
     gene            complement(3293..4069)
                     /gene="dnaQ"
                     /locus_tag="PXD81_001911"
     CDS             complement(3293..4069)
                     /gene="dnaQ"
                     /locus_tag="PXD81_001911"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HDL5537915.1"
                     /translation="MTEEKIIRQVVLDTETTGMNMAGGPPQIGHNIIEIGAVEVINRR
                     LTGRTYHVYIKPPREVDEEAIAVHGITNEFLQDKPVFAEIAEEFLDFIKGAELIIHNA
                     PFDVAFMDQEFSYLPNPPAKTAEMCTVTDSLQMARRMYPGKRNNLDALCDRLGIDNSK
                     RVLHGALLDAEILADVFLMMTGGQISLLGEEETEVVSEVIDIEEEFSAIVLNADEAII
                     LKANDEEEESHLALLKLIEKKSKGNCLWTKALTDTAEISH"
     gene            complement(4157..5827)
                     /gene="ettA"
                     /locus_tag="PXD81_001912"
     CDS             complement(4157..5827)
                     /gene="ettA"
                     /locus_tag="PXD81_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011608528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HDL5537916.1"
                     /translation="MSTQFVYTMHRVGKVVPPKRHILKDISLSFFPGAKIGVLGLNGA
                     GKSTLLRIMAGVDKEFEGEARPQPGIKIGYLPQEPKLAPQQTVREAVEEAVAEVKNAL
                     TQLDEVYAMYADENADFDKLAAKQAELEAIIQAHDGHNLQNQLERAADALRLPDWDAK
                     IEHLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDYEGTVVAITH
                     DRYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKEKRLAQEQAAESARQKSIEKELEW
                     VRQNPKGRQAKSKARMARFEELNSGEYQKRNETNELFIPPGPRLGDKVIEVQNLTKSY
                     GDRTLIDDLSFAIPKGAIVGIIGANGAGKSTLFRMLSGKEQPDSGSIVLGETVVMASV
                     DQFRDAMDDKKTVWEEVSNGQDILTIGNFEIPSRAYVGRFNFKGVDQQKRVGELSGGE
                     RGRLHLAKLLQRGGNVLLLDEPTNDLDVETLRALENAILEFPGCAMVISHDRWFLDRI
                     ATHILDYGDEGKVTFYEGNFSDYEEWKKKTFGEAATQPHRVKYKRIAK"
BASE COUNT         1609 a         1411 c         1284 g         1812 t
ORIGIN      
        1 ctttaaatcc tcttcaaatt ttctgtattc tttataagcg ttaattaaat ttttctcaat
       61 agaaattgga gatggattct tcacaagaac tttatctccc ttaccttgat tttcttttct
      121 atttaaagat ctttttagtc tttgccaata ttgacagttt tttccacata tagggcacgc
      181 agagtcattt agaaaagagt ttactgcatc gtttaatgat agtgatttaa tttcggggcc
      241 tgcatcgagt tcagtgatat taatttcatt aagtgttgta ctcataataa atttttcctt
      301 gttttaagag tttataaatt atttcgcttg ttttcttgcc actgctcggt agccaatatc
      361 tcggcgatag aatttgcctt gccattgaat ttgttcggca agttgtaagg cttgttgttg
      421 agcttcaaat acactgttgc caagtgcggt ggcacaaagt acacgtccac cgttggtgag
      481 cagttttcct tctttttgct caacccctgc taagaaaact ttttgattgg aggtagaggt
      541 cggaatgcct atgatttcat cgcctttacg gtaatcgccc gggtagcctg cggcagctaa
      601 aacaatgcct aatgcggctt gttcacacca ttttgattgc acgctatcca atttgccttc
      661 gcacgctttt aagcacaatt caacgaggtc tgattccaaa cgcatcataa tcggctgagt
      721 ttcagggtct ccaaaacggc agttaaattc aatcactttc ggctgaccgt ttggcataat
      781 cattaagccg gcatagagaa aaccggtgta aggggtattt tcgcttgcca ttccttgcac
      841 ggtcgggtaa ataatttcgt ccattacacg ttggtggatt gcctttgtta ccaccggagc
      901 aggggagtaa gcccccattc cgccggtatt taagccgcta tcgccctcgc caacacgttt
      961 gtggtcttgt gaggttgcca ttggttcgac atttttgccg tccaccatca caataaaact
     1021 ggcctcttct ccgtctaaaa attcctcgat caccacacgg ctgccggctt caccaaaggc
     1081 gttgcctgac agcatatcac gcactgcatc ttccgcctct tgtaaagtca ttgcaacaat
     1141 tacgcctttg cctgcggcta aaccgtctgc tttgattaca atcggtgcac ctttttgttt
     1201 taagtaggca agtgccggtt caatttcggt gaaattttgg tattctgcgg ttggaatctg
     1261 atggcgagcc aagaaatctt tggtaaatgc tttagaacct tctaattgag cagcggcttg
     1321 ggtcgggccg aaaatggtta aaccgtgggc tcgaaaagca tcaaccacgc caactactaa
     1381 cggtgcttca gggccgacaa tcgtcaaacc tacgctattt tgtttagcga atgtaactaa
     1441 ggcaggcacg tcagtggcgg aaatagctac attttcaatg ccgttttcca tcgctgtgcc
     1501 ggcattgccg ggggcgacaa agactttgtc tgcgagcggc gattgacgaa ctttccaagc
     1561 aagagcgtgt tcacgcccac cgttgccgat aattaaaata ttcattggga ttccttgcaa
     1621 aaatgataaa gcaaacgttt gcgtattgta cttgatttgt ttatctgctg acaagcagtc
     1681 gtttttctcg gattttttgc aaaatacgaa attgttgctt gcaaaatagg cttttttcag
     1741 tataatcctg ccgttcagtc acattcgttt tttaatcctt gcttccacaa gttggtgact
     1801 ggctaaagtt ggaggctgct aacccgtaag gatagtcaat gcgtcactac gaaatcgttt
     1861 ttatggttca cccggaccaa agcgaacaag taccagcaat gattgagcgc tacacagcgt
     1921 ctgtaaaaga agctggcggt caagttcatc gcttagaaga ttggggtcgt cgtcaattag
     1981 catacccaat caacaaactt cacaaagcac actatgtgtt aatgaatgta gaagcacctc
     2041 aaagtgtaat cgacgagtta gaaactaact tccgttataa cgatgcagtt cttcgtaact
     2101 taatcgttca cactaaagcg gcagtaactg aagcgtcacc aatggtgaaa gcgaaagaaa
     2161 gcaaagttgc tgaagcggta gctgaagttg aatcagagga agcaggcgag taatttgccg
     2221 attgataatt gtttaatctt aagcggaacg gtttccagct tggttaaaca aagcctaaac
     2281 ccccttggtg tgcctcatta cagtttttgg ttagaacatc gttcaatcca aaccgaagtg
     2341 aatttagaac gccaagcgtg gtgcaaaatc caagtggttt tggcaggcaa tcaatttagt
     2401 tatttaaccc aacaaattaa gctcggtgag agcgtgagaa ttaaaggttt tatccatact
     2461 cacaaagact ttaacggttt aaatcagtta gtcttacaca ccgaacaaat tgaatttata
     2521 gatcaggaga agccaaatgg cacgttattt ccgtcgtcgt aagttctgcc gtttcacagc
     2581 ggaaaatgtt gttgaaatcg attacaaaga tatcgctaca ttaaagaact acatttctga
     2641 aagcggcaag attgttccta gccgtattac tggtactcgt gcgaagtatc aacgtcaatt
     2701 agctcgtgca attaaacgcg ctcgctacct tgcgttactt ccatatactg ataaccacca
     2761 ataatcagga gagagtacaa tgcaagttat tttattagac aaagttgctc acttaggttc
     2821 tgtgggcgac caagtaaccg ttaaatctgg ttttgctcgt aacttcttaa tcccacaagg
     2881 taaagcggtt atggcaactg cggcaaacat tgctcacttt gaagcacgtc gtgcagaatt
     2941 agaagcaaaa gctgcagcag cattagcaac tgcacaagaa cgtgcagcga aaattgcagc
     3001 attagcagca gtatcagtat ctgcaactgc aggtgatgat ggtcgtttat tcggttctgt
     3061 atcagctaaa gacatcgctg aagcattaac tgcagcaggt gttggagttg ctaaatctga
     3121 agttcgttta ggtgaaggtc cacttcgtac aaccggcgaa caccaagtta aaattcactt
     3181 acaccctgaa gtgaatgcaa ctgtagcagt aaacgttgtt gctgaataat cagtagaaat
     3241 agaaaaccca gcgaaagctg ggttttttgt ttgctttatt tattggttag aatcagtgag
     3301 atatttctgc tgtgtcggtt aatgcttttg tccataggca atttcctttg gattttttct
     3361 caatcagttt cagaagtgct aaatgcgact cttcctcttc atcgtttgct ttaagaataa
     3421 ttgcctcatc ggcatttaac acaattgcag aaaattcttc ttcaatatca ataacttcac
     3481 ttaccacttc agtttcttct tcgcctaata atgaaatctg accgccggtc atcattagga
     3541 atacatcagc taagatctcc gcatccagta acgccccgtg tagtacacgc ttgctattat
     3601 caatgccgag cctgtcgcaa agtgcatcta aattattccg tttgccgggg tacattcttc
     3661 gtgccatttg caggctatcg gttacggtac acatttctgc ggtttttgcc ggtggattag
     3721 gcagataaga aaattcttga tccataaacg ccacatcgaa gggggcattg tgaataatta
     3781 attccgcacc tttgatgaaa tccaaaaact cctcggcaat ttcagcaaaa acaggtttat
     3841 cttgcaaaaa ttcattggtg atgccgtgaa ctgcaatcgc ctcttcatct acctcacgcg
     3901 gtggcttgat ataaacgtga taagttctgc cggttaaacg gcggttaatg acttcaaccg
     3961 caccgatctc aataatatta tgcccaatct gtggcgggcc acctgccata ttcattcctg
     4021 tggtttcagt atccagcact acttggcgga taattttttc ttctgtcata ttcaatttaa
     4081 aattgtaggg gtgaaatatt tttatccctt aattctgcat ttgattaggg gcgaaacata
     4141 tttcgcccct acattgttac ttcgcaatcc gtttatattt cactcggtgc ggttgggtgg
     4201 cggcttcacc gaaggttttc ttcttccact cttcatagtc ggagaagtta ccctcgtaga
     4261 acgtcacttt gccttcatcg ccgtagtcta aaatatgggt ggcgatacgg tctaagaacc
     4321 agcggtcgtg cgaaattacc attgcacagc ccgggaactc taagatcgca ttttccaacg
     4381 cacgcagggt ttctacgtct aagtcgttgg tcggctcgtc cagtaacaac acgttaccgc
     4441 cacgttgtag tagcttcgcc aagtgtaaac gaccacgttc accgcctgaa agctcgccca
     4501 cacgtttttg ctgatctacg cctttgaagt tgaaacgccc cacataagca cggcttggga
     4561 tttcaaagtt accaatcgtg agaatatcct gcccgttcga cacctcttcc cacacggttt
     4621 tcttatcgtc catcgcatca cggaactgat cgaccgatgc cattaccacc gtttcaccaa
     4681 gcacgattga accagaatcc ggctgttctt taccagaaag catacggaac aaggttgatt
     4741 tacccgcacc atttgccccg ataatcccga caatcgcccc cttcggaata gcgaacgaca
     4801 agtcgtcaat caaagtgcga tcgccgtaag atttagtcag gttttgtacc tcgatcactt
     4861 tatcacctaa gcgtggaccc ggtggaataa agagttcgtt agtctcgtta cgtttttgat
     4921 attcgcctga gttaagctct tcaaagcgtg ccatacgagc cttacttttc gcttggcgac
     4981 cttttggatt ttggcgtacc cattccaact ctttctcaat cgatttttga cgagccgatt
     5041 ccgccgcttg ctcttgtgcc aagcgttttt ctttctgctc caaccaagaa gagtagttac
     5101 cttcccaagg aatgccttca ccacggtcaa gctctaagat ccagcccgca acgttgtcta
     5161 agaagtaacg gtcgtgggta attgccacta cggtgccttc gtagtcgtgg aggaaacgct
     5221 ccaaccacgc caccgattcc gcatctaagt ggttggtcgg ctcatctaac aataacatat
     5281 ccggtttttc cagcaacaga cggcaaagtg ccacacggcg gcgttcaccc cctgataaat
     5341 gctcgatttt tgcgtcccaa tccggcagac gtaacgcatc ggcagcacgc tctaactggt
     5401 tttgaaggtt atggccatcg tgggcttgaa taatcgcctc taattccgcc tgttttgccg
     5461 ctaatttgtc gaaatcggca ttctcatcag catacatcgc atagacttca tcaagctggg
     5521 ttaaggcgtt tttcacttcc gcaaccgctt cttccaccgc ttcacgcacg gtttgctgtg
     5581 gggcaagttt cggttcttgt ggaagatagc cgattttaat tcccggttgt ggacgagcct
     5641 cgccctcgaa ctctttatct acgcccgcca taatgcgtaa aagcgttgat ttacccgccc
     5701 cgtttaagcc gagcaccccg attttcgccc ccgggaaaaa gctcagggaa atatctttaa
     5761 gaatgtgacg cttcggcggc acgactttgc cgacacggtg catcgtatat acaaattgag
     5821 ttgacattgg ttgtattcct cacaaaaaaa ttcgccttat tttacttgaa tttacagcca
     5881 aaatataggg tggttttggc ttttggtaaa gtaacctttt ccaatagaga tgttatttga
     5941 ggctgcatag tactatgcat ttctcttacc ttgacaggta agagaaacaa atcctgaagg
     6001 aaaaaaattt taaggaagtt attctatgca atatgtatgt ccaaaaacac tcaaattatt
     6061 caacagcatt gttggttgtt ataagggaga agattaattc tctacctcat caaggc
//
LOCUS       DANLYI010000147         6047 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.148, whole genome shotgun sequence.
ACCESSION   DANLYI010000147 DANLYI010000000
VERSION     DANLYI010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 6047)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6047)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6047
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.148"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            <1..152
                     /locus_tag="PXD81_001913"
     CDS             <1..152
                     /locus_tag="PXD81_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009041538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HDL5537917.1"
                     /translation="HYAVVFFVIAIIAALLGFGGIAGSATEIAKILFFVFLILTVISF
                     FFKKK"
     gene            complement(281..883)
                     /locus_tag="PXD81_001914"
     CDS             complement(281..883)
                     /locus_tag="PXD81_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HDL5537918.1"
                     /translation="METLIEFFSSYGYWAVFLVLLACGFGVPIPEDITLVSGGVISGL
                     GYTNVHWMLVVSMLGVLVGDSTMYWLGRIYGEKILKFPLIRNVATPERFATVQERFEK
                     QGWKLLFMARFLPGLRAVVYLVSGITRKVSFVKFLLVDFCAAIISVPIWVYLGDYGAS
                     NLDWLKEQVANGQHLIFAILGIVILFLGWKWYKNKKQYKK"
     gene            complement(885..1904)
                     /gene="purR"
                     /locus_tag="PXD81_001915"
     CDS             complement(885..1904)
                     /gene="purR"
                     /locus_tag="PXD81_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HDL5537919.1"
                     /translation="MATIKDVAKYAGVSTTTVSHVINKTRFVAEDTTKAVWEAIKALN
                     YSPSAVARSLKINTTKSIGMIITTSESPFFAEIVLAVEEFCYSKGYSLFLCNTQNKLE
                     KIQNHLDMLIKKRVDGILVMCSEYTANSFDIFENSNIPMVIMDWGRSDERSDIILDHG
                     VEGGYIATQHLIENGHQDIAVITGNRDKEIARTRFEGFKKALSEAGLSIREEWIQEGD
                     FEPEGGYECMNNLLKSSEKLPTAVFCFNDSMALGAISAITEKGLNVPQDISVIGYDNV
                     HSSRFYAPPLTTIHQSKSRLGSAALELLLERIEDNENAKEPKVLEFFPELVKRKSVRN
                     LNIKE"
     gene            complement(2040..2768)
                     /gene="cmoA"
                     /locus_tag="PXD81_001916"
     CDS             complement(2040..2768)
                     /gene="cmoA"
                     /locus_tag="PXD81_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HDL5537920.1"
                     /translation="MQRDTLFSAPIEKLGDFTFDESVAEVFPDMIQRSVPGYSNIITA
                     IGMLAERFVTDNSNVYDLGCSRGAGILSIRRNVNAKNVQIIGVDNSQPMVERCKTHLS
                     AYHSEIPVTILLDDIRHIEIKNASMVVLNFTLQFLPREDRLALLQKIYQGLNSNGILV
                     LSEKFTFESEEMNELLIDLHHTFKRANGYSELEVSQKRNALENVMRTDSIETHKKRLQ
                     EAGFTQVELWFQCFNFGSMIAIKA"
     gene            complement(2778..3560)
                     /locus_tag="PXD81_001917"
     CDS             complement(2778..3560)
                     /locus_tag="PXD81_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HDL5537921.1"
                     /translation="MTEKSQLNFNYQPSNNHANAQTMIFLHGLFGDLNNLGAIAKPFA
                     ERYSILMVDLRNHGRSFHSDEMNYALIAQDLAQLLTELNIQNAIVVGHSMGGKSAMAL
                     AAFAPELVEKLVVIDIAPVTYTQNRHQQVMAALFAVKEAKPQNRQQAKAIMAEYIRDE
                     GVQQFMLKSFDAQSEQSFRFNLQALKDNYQNVMGWQQVFVDKPTLFIKGALSDYIQAE
                     HTQTILAQFPQAKSFIVANADHWVHAEKTGTVVRAINKFLEK"
     gene            3679..4245
                     /gene="seqA"
                     /locus_tag="PXD81_001918"
     CDS             3679..4245
                     /gene="seqA"
                     /locus_tag="PXD81_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HDL5537922.1"
                     /translation="MKRIEIDDELYQYIASRTQSIGETASDILRRLLRLPQSPQPFVL
                     VQEHMINELKELVKTPSRATKKNESNAEKTVEKLENILNSEHFMNENKNVVRFTMLLA
                     ALYRANPEAFAKATEKVRGNERIYFSQSEAEILATGSGVKAKQIPDSPFWVITNNNTA
                     RKGLILKGVMESMKVPSQLIERIQALFI"
     gene            4255..5673
                     /gene="menE"
                     /locus_tag="PXD81_001919"
     CDS             4255..5673
                     /gene="menE"
                     /locus_tag="PXD81_001919"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HDL5537923.1"
                     /translation="MLINSTRFPTQFWAAHSAQNVAIIWEKGTSDYLNHLPSNITWYS
                     LNQLIAQAIFVLQQQGATPHSLIAYCGSHRLIGLLTYCASLALGAKILMLNPAQTASQ
                     RQAVLDDNGVELIINDADFAKFSAKTTACNAFPTIDFEKPATLTLTSGSSGKPKAVVH
                     SIAAHLYSAEGVCELMAFEQPNSWLLSLPLFHVSGQGIIWRWLFKGATLYVHEDKSDF
                     FLSAQKVSHLSLVPTQLQRYLNQLDSMVSQKCLLGGSMLPAELIAQAQQSGMTTFSGY
                     GMTEMASTICAVENDLNSVGFPLKHRELKLENGEIWVKGKSLGLGYWQKNGQIRPLVN
                     TQGWFATKDRGEWNHKKQLVIKGRLDNMFISGGENIQPEEIEQLIFRSQLVEQIIVLP
                     IEDKQFGHRPVAFLQFKQSDSKNELHKLKIWLSDKLERFKQPVAYYLLDIEKYQKQGL
                     IKLSRTQLQQDLKLKVLKEIYV"
     gene            5666..>6047
                     /locus_tag="PXD81_001920"
     CDS             5666..>6047
                     /locus_tag="PXD81_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HDL5537924.1"
                     /translation="MFRPILKTFVLSLGLMLNSYALAELPTQQAVQTQLDSAKKLDQA
                     DADNKALVQTLEDTLAFLAQIDKQKADNQALNKSIQDSQKALKTSQHNLEKLKEVTIA
                     SVDNLTQKATTDLQKELTSTQSQLE"
BASE COUNT         1934 a         1243 c         1042 g         1828 t
ORIGIN      
        1 tacattatgc agtcgtattt tttgtcatcg ctataatcgc agcgctatta ggatttggtg
       61 gtattgctgg aagtgctact gaaattgcca aaattctctt ttttgtcttt ttaatactta
      121 ctgttatttc gttcttcttc aagaaaaagt gacacagtta aacctaattt tttaagttaa
      181 aatatagttc aaaaaaacag ttggtaatgg atatttataa ttattcttac caactgtttt
      241 gctattatat ctttttaaga tgaaatgctc gtcttttgtt tcacttttta tactgctttt
      301 tatttttata ccatttccag cctaaaaata gaataacaat gcctaaaatg gcaaaaatga
      361 ggtgctgccc attagctact tgttctttta gccaatccaa attagatgcc ccatagtcgc
      421 ctaaataaac ccaaattggc acagagataa tagcggcaca gaaatcaact aataaaaatt
      481 ttacgaagct gacttttctg gtaatacctg aaactaagta aaccaccgcg cgcaagccag
      541 gtaaaaaacg agccataaaa agtagtttcc atccttgttt ttcaaaacgt tcttgcacgg
      601 ttgcaaaacg ttcaggtgtc gcaacattcc tgattaatgg aaatttgaga attttttcgc
      661 catagattct acctagccag tacattgtgc tatcgccaac taatactcct aacatactaa
      721 caaccagcat ccaatgcaca ttggtgtagc ctaatcctga aataacacca ccggatacta
      781 acgtaatatc ttcaggaatc ggcacaccga aaccacaggc aagtagcaca agaaaaacag
      841 cccaataacc atagctacta aaaaattcaa tcaatgtttc catattactc cttaatgttt
      901 aaattccgaa ccgatttacg tttgaccaat tcaggaaaga actctaatac tttaggctct
      961 tttgcgtttt cgttatcttc aatacgttct aataataatt ctaatgctgc cgagcctaag
     1021 cgagattttg actgatggat tgtcgtaaga ggcggtgcat aaaagcgtga gctatgcacg
     1081 ttatcatacc cgatcactga aatatcttgc ggcacattca gccctttttc ggtaatagct
     1141 gaaattgccc ctagtgccat tgagtcatta aaacaaaaaa ccgcggttgg taatttttca
     1201 ctgctcttaa gcaaattatt catacactca taccccccct caggttcaaa atcgccttct
     1261 tgaatccatt cttcacgaat agacaaaccc gcctcagata gcgctttttt aaaaccttca
     1321 aaacgggttc ttgcaatttc tttatctcta ttgccggtaa ttacagcaat atcttgatgc
     1381 ccgttttcaa ttaaatgctg agtcgcaata tagccacctt caacaccgtg atctaaaata
     1441 atatcactgc gttcatcact tcgtccccaa tccataatca ccattgggat attgctgttt
     1501 tcaaagatat caaatgaatt agctgtatat tcggagcaca tcaccaaaat gccatcaacc
     1561 cgttttttga tgagcatatc taaatgattt tgaattttct caagtttgtt ttgagtattg
     1621 cacaaaaata gtgaataccc tttgctatag caaaattcct ctaccgctaa cacaatttca
     1681 gcgaaaaacg gtgattcgct ggttgtaatg atcataccga tagatttagt cgtattaatt
     1741 ttcaaactcc gcgctacagc actaggagaa tagttcagcg ctttaatagc ctcccaaact
     1801 gcttttgtag tgtcctctgc tacaaagcgg gttttgttta taacgtgaga aaccgttgta
     1861 gtcgatacac cagcatattt tgcgacatct tttatcgttg ccataccttt ttcctcctaa
     1921 ataagtagtg gtgcattata actgattcta actgactttt attataaaga aatagataga
     1981 atgcaagcgg ttgcttttta tcaagaattt gcaaaaaatt ttacaaatat caccgcttgt
     2041 taggctttaa ttgcaatcat tgagccaaaa ttaaagcatt gaaaccaaag ttcaacttgt
     2101 gtaaaaccag cttcttgcag acgtttttta tgggtttcaa tactatctgt tcgcatcaca
     2161 ttttccaaag cattacgctt ttggctgact tccaattcac tgtagccatt tgctcgtttg
     2221 aatgtgtgat gaaggtcgat caatagctcg ttcatttcct cactctcaaa ggtaaatttt
     2281 tccgataaca ctaaaatgcc attggaattt aatccttgat aaatcttctg taataatgct
     2341 aggcgatctt ctcttggtaa aaattgcaag gtgaaattaa gtaccaccat tgaagcattt
     2401 ttaatttcaa tgtggcgaat atcatcaagt aaaatcgtca ccgggatctc agagtgataa
     2461 gcacttaaat gggttttaca acgctcaacc atcggctgag aattatctac cccgataatc
     2521 tgcacattct tcgcattcac attacggcga atagataaaa tccctgcgcc tcgtgaacaa
     2581 cctaaatcat aaacatttga attatctgtg acaaaacgct ccgccaacat tccaatagca
     2641 gtaataatat tggaatagcc cggcacggag cgttggatca tatccggaaa cacttcagca
     2701 accgattcat caaaagtaaa atcgcccaat ttctcaatcg gagcggaaaa taatgtatct
     2761 ctttgcattt ttctccctta tttttctaaa aatttattga ttgctctcac aacagttcct
     2821 gttttttctg cgtgaaccca atgatcagcg tttgccacaa tgaaagattt ggcctgcgga
     2881 aattgtgcca aaatcgtttg tgtatgctct gcttgaatat aatccgataa tgcaccttta
     2941 ataaacaagg tcggtttatc cacaaatacc tgctgccacc ccatcacatt ctgataatta
     3001 tcttttagcg cctgtaaatt aaaacggaaa gattgctcgc tttgggcatc aaaagatttc
     3061 aacataaact gttgcacgcc ttcatctcga atatattcag ccattatcgc tttagcttgc
     3121 tgacgattct ggggtttagc ttctttcacc gcaaataagg ctgccataac ctgctgatgc
     3181 cgattttggg tataagtgac cggggcaata tcaatcacta ccaatttctc caccagttca
     3241 ggagcaaatg cagccaatgc catcgcactt tttccgccca ttgaatgccc aaccacaata
     3301 gcattttgga tatttaattc tgtgagtaat tgtgctaaat cttgtgcaat caaagcataa
     3361 ttcatttcgt ctgaatgaaa agatcgcccg tgatttctga gatcaaccat taaaatggag
     3421 taacgttctg cgaatggttt tgctattgcg cctaggttat ttaaatctcc aaacaatcca
     3481 tgtaagaaga tcatcgtctg agcattagca tgattattcg aaggttggta attaaagttt
     3541 aactgcgatt tttctgtcat ataaatgtca ttgttcatat aaattttcag cgatgttcct
     3601 ctataaaaaa caatagaaat gcgtataata gcaagcacta ttaacgcatt taatacccgc
     3661 agatatttga ggatcccgat gaaacgaatc gaaattgatg atgaattata tcagtatatt
     3721 gcaagccgta cacaatctat tggtgaaact gcgtcagata tattacgccg tctattacgc
     3781 ctgccacaat cgcctcagcc ttttgtttta gtacaagaac acatgatcaa cgagcttaaa
     3841 gagcttgtca aaacgccttc tcgtgctacg aaaaaaaatg aatccaatgc agaaaaaacc
     3901 gttgaaaaat tggaaaacat tttaaattcc gagcatttta tgaacgaaaa taaaaatgta
     3961 gtgcgtttta ctatgctatt agccgcatta tatcgagcaa atccggaggc ttttgctaaa
     4021 gcaaccgaaa aagtgcgtgg taatgaacgt atctactttt cgcaaagtga agccgaaatt
     4081 ttagctactg ggagtggagt aaaagccaag cagatcccag attcaccgtt ttgggtaatt
     4141 accaataata ataccgcacg caagggactt attctcaaag gcgtaatgga atccatgaaa
     4201 gtaccaagcc agttaatcga acgcattcaa gcgcttttta tctaattaat tgaaatgctc
     4261 atcaattcta ctcgctttcc tacccaattt tgggctgctc attcagccca aaatgtggcg
     4321 attatttggg aaaaaggaac atcagactat cttaaccacc ttccatcaaa catcacttgg
     4381 tatagtttga atcagttgat tgcgcaagct attttcgtgt tgcaacaaca aggtgctact
     4441 ccgcacagtt tgattgccta ttgtggttct catcggctca ttggcttgct cacttattgt
     4501 gctagccttg ctttgggggc taagattcta atgcttaacc ctgctcaaac agcaagtcaa
     4561 cgacaagcag tcttagatga caatggtgtt gagctcatta ttaatgatgc cgactttgca
     4621 aaattttcag caaaaacgac cgcttgtaat gccttcccta cgattgattt tgaaaaacca
     4681 gctaccctaa ccctaacctc aggctcaagc ggtaaaccta aagcagtggt gcattctata
     4741 gcggcccatc tctatagtgc cgaaggagta tgtgaattaa tggcatttga gcagccaaac
     4801 tcttggctac tgagcttgcc tctgtttcac gtttccggac aaggcattat ttggcgttgg
     4861 ttatttaaag gtgcaacact gtatgttcac gaagacaaaa gtgatttttt tctttcagca
     4921 caaaaagtga gccatctctc tttagtcccc acccaattac aacgctattt aaaccaatta
     4981 gattctatgg tcagccaaaa atgtttactg ggtggatcta tgcttccagc cgaattgatt
     5041 gctcaagcac aacaatctgg gatgactacc ttttcaggct acggaatgac tgagatggca
     5101 tcaaccattt gtgcagtaga aaatgattta aacagtgttg gttttccgtt aaaacatcgg
     5161 gaactaaaat tagaaaatgg tgaaatttgg gtaaaaggca aatcactcgg cttaggctat
     5221 tggcaaaaaa atgggcaaat tcgaccgctt gtaaacacac aaggctggtt tgccactaaa
     5281 gatcgtggcg aatggaatca caaaaaacag cttgtaataa aaggacgatt ggataatatg
     5341 tttatttcag gaggggaaaa tatccaacct gaggaaattg aacaactgat ttttcgttct
     5401 cagcttgtcg aacagattat cgtgttgccg attgaagata agcaatttgg acatcgacca
     5461 gtggcgttct tacaattcaa gcaatctgat tcaaaaaatg aactccataa attaaaaatt
     5521 tggctgtctg ataaattaga aagattcaaa cagcctgttg cctattattt actggatata
     5581 gaaaaatacc aaaaacaagg cttaattaaa ctatcaagaa cacaattaca acaagattta
     5641 aaactcaaag tattaaagga aatctatgtt tagaccgatt ttaaaaacat ttgtgcttag
     5701 tttaggctta atgctaaata gttatgcatt agctgaattg ccaacccaac aggcagtgca
     5761 aacgcagtta gatagcgcca aaaaattaga tcaagccgat gcggataata aggcattggt
     5821 tcaaacactt gaagatacgc ttgctttctt agcccaaatt gataaacaaa aagcagataa
     5881 tcaggcattg aataaatcta ttcaagattc ccaaaaagca ttaaaaacaa gccaacataa
     5941 tcttgaaaaa ttaaaagagg tgactattgc aagtgtcgat aatttaacac aaaaagcgac
     6001 aacagattta caaaaagagt taacctctac acaatctcag ctggaaa
//
LOCUS       DANLYI010000148         5937 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.149, whole genome shotgun sequence.
ACCESSION   DANLYI010000148 DANLYI010000000
VERSION     DANLYI010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 5937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5937
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.149"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            complement(112..1476)
                     /gene="mpl"
                     /locus_tag="PXD81_001921"
     CDS             complement(112..1476)
                     /gene="mpl"
                     /locus_tag="PXD81_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HDL5537925.1"
                     /translation="MKKHIHILGICGTFMGGVAMIARELGYKVTGSDTNVYPPMSTFL
                     AAHGIEIIPNYDVAQLQPAPDLVVIGNAMSRGNPCVEYVLDNQLNYTSGPQWLHDHLL
                     KDKWVLAVSGTHGKTTTTGMLAWILDQNGIDTGFLIGGVAGNFGISARAGNSPFFVIE
                     ADEYDSAFFDKRSKFVHYTPKTLIINNMEFDHADIFDDLKAIQRQFHHLVRTMPQSGC
                     ILSAIADQNVQDTLKMGSYSQLQFIGEQNEWFAKPLNADCSQFEVFHLGVKQGEVHWS
                     IIGKHNMHNALMAIAAAHHAGVEVQGACEALGKFVNANRRLEVKGEVNGITVYDDFAH
                     HPTAIEATIDALRGKVGKNQRILAVLEPRSNTMKMGVHKDEIAPSLHDAEAVFVYQPD
                     IIAWSVSEITNVLSQPAYWSANIDDLVDLVVKEAKPTDHILVMSNGAFGGIHQKLLAK
                     LADK"
     gene            complement(1772..2527)
                     /locus_tag="PXD81_001922"
     CDS             complement(1772..2527)
                     /locus_tag="PXD81_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005613463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ family lipoprotein"
                     /protein_id="HDL5537926.1"
                     /translation="MKLKSFKPMFMLLSVAFLTACSSSINPETGVRDDPLEGFNRAMW
                     KVNYDYLDPYVLKPVAKGWKEYVPSPVKTGLINVANNLDEPVSFVNRLIEGEGKKAMV
                     HFNRFWINTVFGLGGLIDWASLDEGLRLNNGQRGLGESLGAHGVPSGSYIMMPAYGAT
                     TPRQGIGSAVETGYSVLTYVGSPWSIAKFVVQGVDTRSKLLEQDALLEQAQDPYVTFR
                     EAYFQNLEFKVKDGNVEQSQQEQLSEEELKNID"
     gene            2682..2999
                     /gene="metJ"
                     /locus_tag="PXD81_001923"
     CDS             2682..2999
                     /gene="metJ"
                     /locus_tag="PXD81_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011608626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HDL5537927.1"
                     /translation="MADWNGEYISPYAEHGKKSEQVKKVTVSIPIKVLEILTNERTRR
                     QIKNLRHATNSELLCEAFLHAFTGQPLPNDEDLMKDRADEIPEAAKQKMCELGIDPDE
                     WQY"
     gene            3047..3277
                     /locus_tag="PXD81_001924"
     CDS             3047..3277
                     /locus_tag="PXD81_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF997 family protein"
                     /protein_id="HDL5537928.1"
                     /translation="MYQQLTTEARWAVLLTLIYLLGWVGFAYFSPQGRGIFGFPIWFE
                     LSCIFLPVLFSLVATIVVKKVYKNIDLEVNNE"
     gene            3270..4694
                     /gene="panF"
                     /locus_tag="PXD81_001925"
     CDS             3270..4694
                     /gene="panF"
                     /locus_tag="PXD81_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HDL5537929.1"
                     /translation="MNSEMILPLIAYLIFVFGVAFYAYRKRQGGSFISEYYIGGRSMS
                     GFVLAMTTAATYVGASSFIGGPGAAYKYGLGWVLLAMIQVPAVLLSLGALGKKFALLA
                     RKHNSVTINDMLLARYQNKFVVWIAGLALLLSFFAMMTVQFIGAGRLLETTLGIPYQT
                     AVMIFAITVGLYTFIGGFRAVVLTDTIQGLVMIVGTSILLAGVIYATGGVENAMHTLE
                     AINPQLTTPYGIDERPLDFTFMTSFWVLVCFGLIGLPHIAVRSMACKNSQALHRAMII
                     GTVVIAILMFGMHLSGVLGRAVVPDLKVPDQVIPTLMVQVLPPVVAGIFLAAPMAAIM
                     SSIDSMLIQSSSTLIKDLYLAIKPNAINNEKKIKLFSTITTLTFTVLLVFAALTPPEM
                     LIWLNLLSLGGLEATFLWVIVLGLYWEKANATGAIYSMLAGLSCYVLMTTFKIAIFSF
                     HAIVPSLLIGLIAFLIGNRFAPKV"
     gene            complement(4771..>5937)
                     /gene="nhaA"
                     /locus_tag="PXD81_001926"
     CDS             complement(4771..>5937)
                     /gene="nhaA"
                     /locus_tag="PXD81_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HDL5537930.1"
                     /translation="KSEASGGVLLLVFAFAAIVCANTSLSDFYFEFLQTIVSVQFGEF
                     ALNKPLLLWVNDGLMAVFFTLVGLEVKKELFEGSLSSYKKAIFPAIAAVGGMIVPAII
                     YWAINQNHPEYHNGWAIPMATDIAFAVGIVALLGTRVPLALKAFLLALAIIDDLGAIV
                     VIAIFYSQEISVQALTIAAIAIIGLVLLNRFKVTSLCAYLVVGLVLWTSVLKSGVHAT
                     LAGVIIGFCVPLKGKHGERPAEELEHILAPWCAYMILPIFAFANAGVYLLEMGMEQLS
                     SPLPLGIALGLIIGKTLGVFGFSYLAVLFRLASLPQGVNFKQIFAIAVLCGIGFTMSM
                     FLASLAFDVSEAGESITALARLGILLGSAVSAILGYTLLRMATKQNNPENNATY"
BASE COUNT         1703 a         1348 c         1215 g         1671 t
ORIGIN      
        1 acatgtataa atatccagaa ttgattttga atttttcgat gcagatcaca aaaatagaga
       61 gttatacaaa caagcggtaa aattttgtaa aaaatttacc gcttgtaaag gttacttatc
      121 agctaatttg gcaagtagtt tttgatgaat accgccaaag gctccgttgc tcatcactaa
      181 gatgtgatcg gtgggtttgg cttctttgac gactagatcg actaaatcat caatatttgc
      241 cgaccaatac gccggttgcg ataacacatt ggtaatctcg ctcacactcc acgcaataat
      301 atcaggctgg taaacaaaca ctgcttcggc atcgtgtagg cttggggcta tttcatcttt
      361 atggacaccc attttcattg tatttgaacg aggctctaac actgccaaaa tgcgttgatt
      421 tttgccgact ttaccacgca gagcatcaat agtcgcttct atcgcagttg ggtggtgagc
      481 gaaatcatca taaacggtaa tgccgtttac ctcgcctttc acttccaaac ggcggttagc
      541 attcacaaac ttgcctaatg cctcgcaagc cccttgcact tcaacccctg cgtgatgagc
      601 ggcggcaatc gccatcagag cattatgcat attgtgcttg ccgataatcg accaatgcac
      661 ctcgccttgt ttaacgccta agtgaaacac ctcaaactgt gagcaatcgg catttaacgg
      721 tttagcaaac cattcgttct gttcgccaat aaattgaagc tggctgtaac tgcccatttt
      781 tagtgtatct tgcacgtttt gatcagcgat tgccgacaaa atacagccac tttgtggcat
      841 cgtgcgaaca aggtggtgga actggcgttg aatcgctttt aaatcatcaa aaatatcggc
      901 gtggtcaaat tccatattat tgataatcag cgttttcggg gtgtaatgca caaatttaga
      961 acgcttatca aagaacgcag aatcgtactc gtccgcctca atcacgaaga atggactgtt
     1021 accggcacga gccgaaatgc cgaaattacc ggcaacaccg ccaattaaaa agccggtatc
     1081 aatcccattt tggtctaaaa tccacgctaa cattcctgtt gtggtagttt tgccgtgcgt
     1141 gccggaaacc gccagcaccc atttgtcttt aagcaaatga tcgtgcaacc attgcgggcc
     1201 ggaggtgtaa ttgagttggt tgtccaacac atattccacg cacggattac cacggctcat
     1261 cgcattacca atcaccacta aatcaggagc cggttgcagt tgagcaacat cgtaattcgg
     1321 gataatttca ataccgtgag cagctaaaaa ggtactcatc ggtgggtaaa cattagtgtc
     1381 tgagccggtc actttatagc caagctcacg ggcaatcatt gccacaccgc ccataaaggt
     1441 gccgcaaatg cctaaaatat ggatatgttt tttcatcttt ttatatttaa aatgttagaa
     1501 tcatcccctc tccccttgtg ggagagggat agattttttc ttcgtgcaga agaaaaaatc
     1561 agggagaggg gtaagcggtc atttttaggc ttaatattgt aagctccctc tctctgaaaa
     1621 atatttctga acgaaatatt tttcggtctc aatactcgct acgctcgctc ctgtggagcc
     1681 gttggcaaag ccaacgttca aatgtaaaca tttgtcccac aaggggagag gtgcgcttta
     1741 gatgaggcgt accatccctc gttcacatca attaatcaat attcttcaac tcttcttccg
     1801 aaagctgctc ttgttggctt tgctcaacat tgccatcttt cactttaaat tcaaggtttt
     1861 ggaaataagc ttcacggaag gtaacataag ggtcttgagc ttgctctaac aacgcatctt
     1921 gttctaataa tttagaacgg gtatctaccc cttgtaccac aaatttagcg atagaccaag
     1981 gtgacccaac ataagtcagc accgaataac cggtttccac cgcactacca ataccttgac
     2041 gaggtgtggt tgctccataa gccggcatca taatatagga gccggaaggc acgccgtgag
     2101 cgcctaaact ctcacctaaa ccacgttgtc cgttatttaa acgtaacccc tcatctaaac
     2161 tcgcccagtc aattaaccca cctaaaccaa acacagtgtt gatccagaag cggttgaagt
     2221 gaaccatcgc ttttttacct tctccttcaa ttaagcggtt tacaaagctc accggctcat
     2281 caaggttatt agcaacatta attaagcctg ttttaaccgg gcttggtaca tactctttcc
     2341 aacctttagc taccggtttt aacacataag ggtctaaata gtcgtaattg actttccaca
     2401 tcgcacggtt aaacccctca agtgggtcgt ctcgcacgcc ggtttctggg ttaatgcttg
     2461 atgaacaagc ggttaaaaat gccacactga gtagcataaa catcggtttg aacgacttga
     2521 gtttcataaa ttgaaccttt tgcagagaaa tttgtccatt gtagcgaagt tgataaaaaa
     2581 tacaaaacaa aaagccgttt tgcctctaga aatctagacg tctagattga gatatagttc
     2641 gcttttgatt acaatacagg caacatttaa ggagcaaaca aatggcagac tggaacggcg
     2701 aatatattag cccttatgcc gaacacggca aaaaaagtga acaagtcaaa aaagtgacgg
     2761 tatccattcc aattaaagta ttggaaattt taactaatga gcgaacccgt cgccaaatca
     2821 aaaatcttcg tcacgcaacc aacagcgaat tattgtgcga ggcttttttg cacgccttta
     2881 ccggtcagcc tttgccgaat gatgaagact tgatgaaaga cagggcagat gaaatccctg
     2941 aagcggcaaa acaaaaaatg tgtgagctgg gaatagaccc ggacgaatgg caatattaga
     3001 cacaccttgc aactgcgagg ttctttttat gttttaagga gactttatgt atcaacaatt
     3061 aacaacagaa gcacgttggg ctgtgttgct tacgctgatt tatctgctcg gctgggtagg
     3121 ttttgcttac ttttcaccgc aaggcagagg cattttcggc tttccgattt ggtttgaact
     3181 ttcttgcatt ttcttacccg tactatttag cttagttgcc accattgtcg tcaaaaaagt
     3241 gtataaaaat atcgatcttg aggtgaataa tgaatagtga aatgatttta cccctgattg
     3301 cttatttaat ttttgtgttt ggcgtagcgt tttatgctta ccgtaaacgc caaggcggaa
     3361 gctttatttc ggaatactat atcggtgggc gttcaatgtc gggctttgtg ttagcaatga
     3421 caaccgccgc cacttatgtg ggggcaagtt cctttatcgg tgggccgggt gctgcctata
     3481 aatacggttt aggctgggtg ttactggcaa tgattcaagt gcccgcagtg ctactctctc
     3541 ttggagcatt aggcaaaaaa tttgccttac ttgcccgcaa gcataatagt gtcaccatta
     3601 acgatatgtt attagcccgt taccaaaaca aatttgtggt ttggattgca ggactagcct
     3661 tattactttc gttttttgcg atgatgacgg tgcaatttat cggtgccggt cgcttacttg
     3721 aaaccacact cggaatccct taccaaacag cggtaatgat ttttgccatc acagtcgggc
     3781 tttatacctt tatcggcggt tttagagcgg tggtgctaac cgatactatt caaggtttag
     3841 taatgatagt cggcacaagc attttactcg ctggtgtgat ttatgccaca ggcggagtgg
     3901 aaaatgcaat gcacaccctt gaagccatta acccacaact taccacccct tacggcattg
     3961 acgaacgccc gcttgatttc acctttatga cttcattttg ggtattggtt tgctttggtt
     4021 taatcggctt accgcatatt gcggtcagaa gtatggcatg caaaaacagc caagccttgc
     4081 accgagcaat gattatcggc acggtagtca ttgcaattct aatgtttgga atgcaccttt
     4141 caggcgtatt aggcagagcg gttgtgcctg atctcaaagt gccggatcaa gtcattccaa
     4201 cactaatggt acaagtgcta ccaccggtgg ttgccggtat tttccttgcc gccccaatgg
     4261 cagcgattat gtcttcgatt gactcaatgt tgattcagtc gtcttcaacc ttaattaaag
     4321 atctctatct ggcaattaag ccgaatgcca ttaataacga gaaaaaaatc aaattgttct
     4381 ccactattac tactcttact tttacggtat tactcgtgtt tgccgcccta accccgcctg
     4441 agatgttaat ttggctgaac ctactctctt taggtggttt agaagcgact ttcctatggg
     4501 taattgtact agggttgtat tgggaaaagg caaatgccac cggtgctatt tactcaatgc
     4561 tggcaggctt aagttgttat gtattaatga ctacctttaa gattgctatt tttagcttcc
     4621 acgccattgt gccttcactg ctaattggtt tgattgcttt cttaatcggc aaccgatttg
     4681 ctcctaaggt gtaaaataaa ataggcatag tggtatggaa gttcactatg cctattttgc
     4741 aaaatttccg ctcaattcaa ccgcttgtag ttaataagtg gcattatttt ctggattatt
     4801 ctgcttagtc gccattctaa gtaacgtata acctaaaatc gctgaaaccg cagagccgag
     4861 taaaatacct aatcgagcta gagcggtaat actttcccca gcctcactta catcaaaggc
     4921 aagacttgct aagaacatcg acattgtaaa accgataccg catagcaccg caatggcaaa
     4981 aatttgctta aaattaaccc cttgcggcag gctggcaagg cggaataata ctgctaaata
     5041 actaaaaccg aatacaccta aggttttgcc gataattaag ccgagcgcaa tacctaacgg
     5101 caatggtgat gagagttgct ccattcccat ttctaataag taaacgccgg cattagcgaa
     5161 ggcgaagatc ggtaaaatca tataagcaca ccaaggggca agaatatgtt ctagctcctc
     5221 tgccggtcgt tccccatgtt tacctttcag cggtacgcag aagccgatga tcacgccggc
     5281 aagggtagcg tgaacccctg attttaaaac ggaagtccat agcactaagc ctactaccaa
     5341 ataagcacag agtgaggtca ctttaaagcg gttaagtagc actaagccga taatcgcaat
     5401 cgcggcaata gttaaggctt gcacactgat ttcttgcgaa tagaaaatgg cgattaccac
     5461 aatcgctcct agatcatcaa taatcgctaa tgccaacaaa aatgccttta aagccaatgg
     5521 cacacgagtg cctaataacg ccacaatgcc caccgcaaag gcaatatcag ttgccattgg
     5581 aattgcccaa ccattatgat attcagggtg gttttgatta atcgcccaat aaataatcgc
     5641 tggaacaatc ataccaccta ctgctgctat cgcaggaaaa atagctttct tataacttga
     5701 aagcgaacct tcaaacaact ctttttttac ttccagcccc accaacgtaa aaaacactgc
     5761 cattaagcca tcgttaaccc agagtaatag cggtttattc aaggcaaatt caccaaactg
     5821 tacactaaca atcgtttgta aaaactcaaa atagaaatca cttaaggagg tgttggcgca
     5881 cacaattgct gcaaaagcaa aaaccagaag caatacgcct cctgaggctt cagattt
//
LOCUS       DANLYI010000149         5803 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.150, whole genome shotgun sequence.
ACCESSION   DANLYI010000149 DANLYI010000000
VERSION     DANLYI010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 5803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5803
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.150"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            1..1818
                     /locus_tag="PXD81_001927"
     CDS             1..1818
                     /locus_tag="PXD81_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme anaerobic degradation radical SAM
                     methyltransferase ChuW/HutW"
                     /protein_id="HDL5537931.1"
                     /translation="MIIKDTHWTSQQASPNAFPERQALMPIWGGKNIERNQWQSLWLE
                     QAPNALLQDGLAYFHIPFCASHCIFCGFYRNAWKENYSQIYTDRVISELRYESSLWRG
                     QGKIHAVYFGGGTPTALHTEDLVRLIRACYRYLPLAEDCEFTIEGRISHFDIDKAKAC
                     IEAGVNRISIGIQTFNSEIRKRLGRKHSGEEAQTYLKALSELNAVIVADLIFGLPNQT
                     DDIWQNDIEIATSLPISGLDIYAFNNYPFLPINRLIEHGKLPAPAGFDTQSLHYAYAV
                     EKLKAQGWQQVSNNHFAFPNRGERNRYNTLVKSNMPCLAFGSGAGGNFGGLSYQVQSD
                     LKTYLDSPLDQKSLSFLSKHGEHKALLGVIQHSMELGYLDTTPFEQNAKAEKLLQQWE
                     ALDLLKREKNQVYLNTSGRYWSPTLIRKLMLTLPQSSKDNNMITPLTKELENKLRTNL
                     KENPGQILEMLAGMNQCSLESVINCLPKEMVMQTGGERFVEIMQAIAGWNEAVTFIIH
                     TPDAIAEVTAKLPQGKIGRGFYNFEHGVDGGIHGHLRYENCAAIYLLDRPFMGKRTVA
                     LTFINQSGEAMFKIFVGRDEQGQLRENQIQAMRKLIGGC"
     gene            1821..2438
                     /locus_tag="PXD81_001928"
     CDS             1821..2438
                     /locus_tag="PXD81_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDL5537932.1"
                     /translation="MILVCGANGLLGRAICDKLPREKTIAVLRKDAMDTFFSERNIAV
                     EIADLLDLESCNALLQKTQPEILITVVGGKNEQGIRSDKTANINLIKAVEKYSPTTKV
                     VLVTSVGCAEQWDLISPLAQQALGEALKAKTEAEAYLQQTALNWLIIRPGGLNNDEVN
                     DEYQIVRDLPNERKMYVSRKSVATGIVRLLDSTENHQIFSIINRG"
     gene            complement(2522..3133)
                     /locus_tag="PXD81_001929"
     CDS             complement(2522..3133)
                     /locus_tag="PXD81_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentahemic C cytochrome"
                     /protein_id="HDL5537933.1"
                     /translation="MKSLSKIAFCFLAFIMPTFCFAGSNSYTTETIPLFADENLTQKI
                     GQLEAGAEIKLVQTTAKAAEIELNLWRKSKGFGRIWYHSFSKNITDAVFEKSFISNNP
                     LLEIVESKEDPNTGLVWQKVKMNVWVENAEFTQDLTNFWANTKNTYKTECSVCHKQRD
                     PKMHDANEWIAVFNGMVGFTDMDEATQKKVLRYLQMNAASSSQ"
     gene            3328..4236
                     /gene="glyQ"
                     /locus_tag="PXD81_001930"
     CDS             3328..4236
                     /gene="glyQ"
                     /locus_tag="PXD81_001930"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017806840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HDL5537934.1"
                     /translation="MSTKFNVKTFQGMILALQDYWASKGCTIVQPFDLEVGAGTSHPM
                     TCLRALGPEPMAFAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLDS
                     LKMLGFDPTQNDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPV
                     TGEITYGLERLAMYIQGVDSVYDLVWSDGELGKTTYGDVFHQNEVEQSTYNFEYADVD
                     FLFQCFEQYEKEAQYLLGLEKPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILR
                     IRALTKGVAEAYYASREALGFPGCKK"
     gene            4316..4576
                     /locus_tag="PXD81_001931"
     CDS             4316..4576
                     /locus_tag="PXD81_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5537935.1"
                     /translation="MKPILYFADWCPDTAPFVAELDRLGIEYEPCDITKGGSTLKPFL
                     KLRDTHSAFDHAKANGYIGIPALLIEGEKVVLDLAELEGIFG"
     gene            4619..5653
                     /locus_tag="PXD81_001932"
     CDS             4619..5653
                     /locus_tag="PXD81_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1016 family protein"
                     /protein_id="HDL5537936.1"
                     /translation="MKTVIADNSAEFVNEISQLIQSSKQRMTVAVNAELTLLYWHIGK
                     RINDYILQGERAEYGQEVVKNLAQSLTEQFGKGWSKRHLNYTMQFATTFPNLEIVHAL
                     RAQLSWTHFKQIIHIDDPTKRGFYATMAAQERWSTRTLEDRIGSLLFERTAISKKPDE
                     TIIAELSLLREKGEYNQSMVLKDPYILDFLDLNDRYMERDLEDAILREIEQFLLELGA
                     GFTFVARQKRIQIDNDDFYIDLLFYNRKLKRLIAIELKTESFKHSHKSQMELYLAWLA
                     KYEQEADENPPLGMILCTGKKQEQVELLDLKNSDIHIAEYMTVLPSKAVLEQRLHLAV
                     ERAKERFLLK"
BASE COUNT         1778 a         1102 c         1294 g         1629 t
ORIGIN      
        1 atgattataa aagatacaca ctggacatct caacaagcct ctccaaatgc tttcccagaa
       61 cgacaggcac ttatgcctat ttggggtggg aaaaatattg aacgcaatca atggcaatct
      121 ttatggctgg aacaagcacc aaatgcgtta ttacaggatg gtttagctta tttccatatt
      181 ccgttttgcg ctagtcattg tattttttgt ggtttttatc gcaatgcttg gaaggaaaat
      241 tatagccaga tttatactga tcgtgtgatt agtgaacttc gatatgaatc ttcactatgg
      301 cgaggacaag gtaagattca tgccgtttat tttggtggag gaacgccaac agcgttacat
      361 acagaagatt tagtacgatt gattcgagct tgttatcgtt atttgccact cgctgaagac
      421 tgtgaattta ctattgaggg tagaattagc cattttgata tagataaggc taaggcttgt
      481 attgaagcag gcgtgaaccg tatttctatt ggtattcaga cttttaactc cgaaattcgc
      541 aaacgtttag ggcgcaaaca tagtggcgaa gaggctcaaa cttatttaaa agccttatcg
      601 gaattaaatg ctgtgattgt tgctgattta atttttggct tgccaaacca aacagatgat
      661 atctggcaaa acgatattga aattgcgact agtttaccga tctcaggctt agatatttac
      721 gcctttaata attatccttt cctgcctatt aatcgcttaa ttgaacatgg aaaattacct
      781 gcgcccgcag ggtttgacac ccaaagctta cactatgctt atgcagtaga gaagttaaaa
      841 gcacaaggtt ggcagcaagt gagcaataac cactttgctt ttccaaatcg aggtgaacgc
      901 aatcgctata atactttggt taaatcgaat atgccttgtt tggcatttgg ctccggtgcg
      961 gggggaaatt ttggtgggct gagttaccaa gtacaatctg atttaaagac ctacttagat
     1021 tcgcctcttg atcagaaaag cctttctttt ttgagtaaac atggtgaaca taaagcactg
     1081 cttggtgtga tacaacatag catggaattg ggttacttag atacaacgcc ttttgaacaa
     1141 aatgcgaaag ctgaaaaatt attgcaacaa tgggaagcgc ttgatttact gaagcgtgaa
     1201 aaaaatcaag tatatctcaa taccagtggt cgttactggt caccaacatt aattcgtaaa
     1261 ctaatgttaa cacttcctca atcaagtaag gataacaaca tgataacgcc attaactaaa
     1321 gaattagaaa ataaattgcg gacaaattta aaagaaaacc cagggcaaat tttagaaatg
     1381 ctggcaggaa tgaatcaatg tagcttggaa tcggtaatta actgtctgcc caaagaaatg
     1441 gtaatgcaaa caggtggtga gcgttttgtg gaaattatgc aggcaattgc tggttggaat
     1501 gaagctgtga cttttattat tcatacgcct gatgcgattg cagaggtaac tgctaaattg
     1561 ccacaaggta aaattgggcg aggtttttat aattttgaac atggtgtaga tggaggaatt
     1621 cacggtcatt tacgctatga aaattgtgct gctatttact tacttgatcg cccatttatg
     1681 ggaaaaagaa cggtggcgtt aacttttatc aatcaaagcg gtgaggcgat gtttaagatt
     1741 tttgttgggc gtgatgaaca aggtcaatta cgagaaaatc agattcaagc catgcggaaa
     1801 ttaattggag gatgctagtc atgattttgg tttgtggggc aaatgggttg ttaggacgag
     1861 caatctgtga caaattacct agagagaaaa cgatagccgt cttacgaaaa gatgctatgg
     1921 atacgttttt cagcgaacga aatatagcag tagaaatagc agatttatta gatttggaat
     1981 cctgcaacgc cttgttacaa aaaacacaac cggaaatctt gattacggta gtaggaggaa
     2041 aaaatgaaca aggtattcgt agcgacaaaa cagcaaatat taatttgata aaagcagttg
     2101 aaaaatattc accaactacg aaagtagttt tagttactag tgttgggtgt gcagaacaat
     2161 gggatttaat tagcccatta gcacagcaag ctttaggtga agcactaaaa gccaagacgg
     2221 aagctgaagc ttatttacag caaacagcat taaattggct gattattcga cccggtgggt
     2281 taaataatga tgaagtaaat gatgagtatc agattgtgcg tgatttacct aatgaacgga
     2341 aaatgtatgt aagccgtaaa tctgtcgcaa caggcatagt gcgtttactg gatagtacgg
     2401 aaaatcatca aatcttctct attattaata ggggatagta tatttcaacg aaggcaagcg
     2461 ggcatatttt atggttattt tgcaactata aaacagaccg cttgtgagat ctttttctat
     2521 tttattgaga actggatgcc gcattcatct gcaagtagcg aagcaccttt ttctgggtag
     2581 cttcgtccat atcggtaaaa ccgaccattc cgttaaatac tgcaatccat tcgtttgcat
     2641 catgcatttt aggatcacgt tgtttatggc acacgctgca ttctgtttta taggtatttt
     2701 tggtgttcgc ccaaaagtta gtgagatctt gggtaaattc ggcattttcc acccacacgt
     2761 tcattttcac tttttgccat accaagccgg tgttcggatc ttccttgctc tctacaatct
     2821 ctaacagtgg gttgttgctg ataaaagatt tctcaaatac cgcatcggta atatttttac
     2881 tgaaagaatg ataccaaatc cgcccgaaac ctttgctttt tcgccataaa ttcagctcaa
     2941 tttcagcggc ttttgcggtt gtttgcacca atttaatttc tgctccggct tctagctgcc
     3001 cgattttctg tgtcaaattt tcatcggcaa ataaagggat tgtttccgtg gtgtaactgt
     3061 ttgaaccggc aaaacaaaat gttggcatga tgaatgccaa gaaacagaaa gcgattttcg
     3121 ataatgattt catcataaac cccacaaatt agatgagaaa ttaggttata tgttacacaa
     3181 attgagcaaa aaagcgttga tttacatcat aaaactgatt ttatgtcgat ttcttaattt
     3241 tttgcaaaaa aatcccaaaa aatgactgct tacaagctat aataaccagt atttttcgac
     3301 catttttcaa attcaagaga aaataaaatg agtacaaaat tcaacgtaaa aacctttcaa
     3361 ggaatgattt tagccctgca agactactgg gcaagcaaag gttgcaccat tgtgcagccg
     3421 ttcgatttag aagtgggggc aggcacctcg cacccaatga cctgcctgcg tgcgcttggc
     3481 cctgagccaa tggcgtttgc ctatgtgcaa ccatctcgcc gcccgacaga cggtcgctac
     3541 ggcgaaaatc cgaaccgttt acagcactac taccagttcc aagtggtgat taaaccttcg
     3601 ccggataaca ttcaagaact ttacctcgac agcctgaaaa tgctcggttt cgacccaacc
     3661 caaaacgaca ttcgttttgt ggaagacaac tgggaaaacc caacgcttgg ggcgtggggc
     3721 ttaggttggg aagtgtggct gaacggaatg gaagtgaccc aattcaccta cttccagcaa
     3781 gtcggcggct tggaatgtaa accggtgaca ggcgaaatca cctacggctt agagcgtttg
     3841 gcgatgtaca ttcaaggcgt ggacagcgtg tacgacctcg tgtggtctga cggcgaactc
     3901 ggtaaaacca cctacggcga cgtattccac caaaacgaag tggagcaatc cacctacaac
     3961 tttgaatacg ctgatgtgga cttcctgttc caatgcttcg agcagtacga aaaagaagcc
     4021 caatacctgt taggcctaga aaaaccattg ccattgccgg catacgaacg cattttaaaa
     4081 gccgctcaca gctttaactt attagatgct cgtaaagcga tctcggtaac tgaacgccaa
     4141 cgctacattc tacgcattcg tgccttaacc aaaggcgttg ctgaagcgta ctatgccagc
     4201 cgtgaagcgt tggggttccc ggggtgtaaa aaataatcgt agggacgcca cgcttggcgt
     4261 ccgtcaataa attacaaaac ggacacgaag catcgtgtcc ctacaaggat ttacgatgaa
     4321 accgatttta tacttcgccg attggtgtcc ggataccgcc ccatttgtgg cagaactaga
     4381 ccgcttgggc attgaatatg agccgtgcga catcaccaaa ggaggctcaa cacttaaacc
     4441 atttttgaaa ctgcgagaca cgcattctgc ctttgaccac gccaaagcca acggctatat
     4501 tggcattccg gcattgctga tcgaaggcga aaaagtcgtg ctggatttgg ctgaattaga
     4561 gggtattttc ggctaacaag cggttgaatt tggcggaaat gttgcaaagg agccaataat
     4621 gaagacagta atagcagata attcggctga atttgtgaat gaaatttcac aacttattca
     4681 atcgagtaag caacgaatga ccgttgcggt taatgcagaa ttaaccttgc tttattggca
     4741 tatcggaaaa cggatcaatg actatatttt gcagggtgaa cgtgcggaat atgggcaaga
     4801 agtggtgaaa aatcttgctc aaagtctgac ggagcaattt ggtaaaggtt ggagtaaacg
     4861 ccatttaaat tatacaatgc aatttgctac aactttccct aatttggaaa ttgtgcacgc
     4921 actgcgtgca caattaagct ggacacattt taagcagatt attcatattg atgatccaac
     4981 aaagcgaggg ttctatgcaa caatggctgc acaggagcgt tggagtacaa ggacattaga
     5041 agatcgcatt ggctcattat tgtttgaacg cacggcaatt tccaaaaagc ccgatgaaac
     5101 cattattgct gaattgagtt tgctacgtga aaaaggcgaa tataatcagt caatggtgtt
     5161 gaaagatccc tatattttgg attttctcga ccttaacgac cgttatatgg aacgtgatct
     5221 cgaagatgcg attctacgag agatcgaaca gtttttactg gaacttgggg cgggttttac
     5281 ctttgtcgcc cgccaaaaaa ggattcagat tgataatgat gatttctata tcgatctgct
     5341 gttttataac cgtaaactca aacgcctgat tgcgattgaa ctaaaaaccg aaagcttcaa
     5401 acattcccac aaaagtcaaa tggaactcta tttggcgtgg ctggcgaaat acgagcaaga
     5461 agcagacgaa aacccaccgc ttggaatgat tctctgcacc ggtaaaaagc aggagcaagt
     5521 ggaattgttg gatttgaaaa attccgatat tcatattgcg gaatatatga ccgtgttgcc
     5581 gagtaaagcg gtattggaac aacggttgca tttggcggtg gaaagggcga aagagcggtt
     5641 tttactaaaa tagcaagcgg ttaaaatcag cggaaatgtt gcaaaatgaa agtatgatgg
     5701 aaacgatttg gttgcatatt ccgttggtgg gtggttagag attttcaaat ctcccccctc
     5761 cccctctttg gctaaagagg gggcgatcat ttagtgagta att
//
LOCUS       DANLYI010000150         5801 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.151, whole genome shotgun sequence.
ACCESSION   DANLYI010000150 DANLYI010000000
VERSION     DANLYI010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 5801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5801
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.151"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            complement(<1..389)
                     /locus_tag="PXD81_001933"
     CDS             complement(<1..389)
                     /locus_tag="PXD81_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDL5537937.1"
                     /translation="MFSDNKALHLGKIYHLIEQFELISRTDLAKLSGLAPASVTNLTK
                     ILIDNHFILERTVQTTLSRGRPSVGLAVSNFFWQLLCVTVSSHKIEISLCKLNGAEIY
                     KQDYTISAENYPRLNDFILNFITNFRQL"
     gene            523..1641
                     /gene="rnd"
                     /locus_tag="PXD81_001934"
     CDS             523..1641
                     /gene="rnd"
                     /locus_tag="PXD81_001934"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HDL5537938.1"
                     /translation="MESLIHYQWVETNEQLEAVCEQARKTKVVALDTEFIRTRTYYPI
                     LGLIQLFDGQQVSLIDPNTISDFSPFVSLLADKNIIKVLHACSEDLEVFEYQFKQLPE
                     PMLDTQVMAAFAGIGISMGFAKLVSHYLNIELDKGASRTDWLARPLSAEQLQYAAADV
                     WYLLPVYEKLAQALTKSTWQSAVAEECVALLKKAKQPINKEKAYKNIANAWRLNREEL
                     AILQVLAKWRIEEAQKRNLALNFVVKEASLVQIAKSCPKHTSSLLEFMHPNEVRIHGK
                     KILWLVEQGKAIESNDYPELIQRLVDEKGYKYYMQAMLQKLLEIQPLDIVPELVASKR
                     QLNQLFKWYIDGKPSEKIPELLIGWRKPFGEQLLSVLS"
     gene            1698..2180
                     /gene="lrp"
                     /locus_tag="PXD81_001935"
     CDS             1698..2180
                     /gene="lrp"
                     /locus_tag="PXD81_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005695827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HDL5537939.1"
                     /translation="MENKKPPKALDAIDLKILNELQRNGKISNIELSKRVGLSPTPCL
                     ERVKRLEKNNVIMGYKALLNPELLDAPLLVIVEITLVRGKPDVFEEFNKAIQQLDEIQ
                     ECHLVSGDFDYLLKTRVADMVSYRKLLGTTLLRLPGVNDTRTYVVMEEVKQTNYLLLK
                     "
     gene            2191..4851
                     /locus_tag="PXD81_001936"
     CDS             2191..4851
                     /locus_tag="PXD81_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009875390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HDL5537940.1"
                     /translation="MIEQLKPHLKGKENLIKLMLLLTGFLGVYLLIAWASYSPLDNAW
                     SVASSITQTTLNKTGHFGAWSIDMLYAMFGKVAVLVPIALIIFSIYSLGMGLSVDMKW
                     KTVFIRLASFLLLMVGLAGLFSVLFSNSAYYLSGGFVGGVWETILSDTIGQFGALLIA
                     MLFTIAGLYFCSVQSLLPILTQFYEWIMAKSEESKTAESLQHLQNTEQKQPLVTENEA
                     VEVVDDHKPQFTDISAFKRPNISGLRHSENVEIDNREMYEIERDLELPTINILIQPDM
                     VNQVAEQATSGYELPNEESNVLQMPTIRLNTAIENEVDTIENSSSLEDLKIAESMLPQ
                     IQLDNDIEDTEEAIDDVREITPEFMVTPPKAMKDVSEQPAYPKGYGDTLIHPLLQRKV
                     ITEKPTTPLPTLDLLDKAPIQSQQITEQEIRETSVRLEAELANFGVKATVEDVLVGPV
                     VTRYEIQPAAGVKASKITNLASDIARGLMFKAIRITEVIPNKPYMGIETPNKHRETVW
                     LRDVLDSDEFRNTTATLPMALGKDISGEPVVVDMAKMPHLLVAGQTGGGKSVGVNTMI
                     LSLLFKLTPEQVRFIMIDPKVVELSIYNDIPHLLTPVVTDMKKAANALRWAVEEMERR
                     YLLVSHLQVRNIEGYNAKIDQAAAMNLPIPDPTWRPGDSMDSLPPPLQKLSYIVLIVD
                     EFADLMMSAGKEVEEYIMRIAQKARAVGIHLILATQRPSTDVITGVIKANIPSRIAFT
                     VASQIDSRTILDSGGAEALLGRGDMLYSGAGSPDIIRIHGAFMKDEEVQRVADNWRAR
                     GKPNYLESIVESRSEEADGKNDGGTGDLDPLFDEVVEYITETGSVSISNIQRRFSLGF
                     NRAARIVDQMEAQGIVSEPLKGGKREVLAR"
     gene            5003..>5801
                     /locus_tag="PXD81_001937"
     CDS             5003..>5801
                     /locus_tag="PXD81_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005600761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HDL5537941.1"
                     /translation="MRLDDIKESKNVEDRRFNNVGRTKVGRGKGGILTFIIVLVGAYY
                     GVDLTGLMGGGVEYEEQTSALNESEEKQLRTLSAKVLTTTENIWTSYFQQNGLTYKEP
                     SLVLYRGAIQTACGTGQSAMGPFYCPVDQKVYLDLSFYDDMRKKLRASGDFAFSYVIA
                     HEVGHHVQNLLGITSKTQRAQMKASSKAEANQISVNVELQADCFAGVWGHQLAKANRL
                     EEGDVEEAFNAAQAVGDDRLQQQSRGYVVPDSFTHGSSAQRLAWFRKG"
BASE COUNT         1844 a         1019 c         1292 g         1646 t
ORIGIN      
        1 agttggcgga aatttgtaat aaagttaaga ataaaatcat ttagcctagg ataattttct
       61 gctgagattg tataatcttg cttataaatt tctgcaccat tgagtttaca aagtgaaatt
      121 tcaattttat gagatgaaac agtaacacaa agtagttgcc aaaaaaaatt agaaacagct
      181 aatccaacgg aggggcgacc tctggataaa gtagtctgaa ccgtacgttc taaaatgaag
      241 tggttatcta ttagtatttt agtcagattt gttactgaag ctggggctaa ccccgatagt
      301 ttagctaaat ctgttcttga aatgagttca aattgctcaa ttaaatggta tattttaccc
      361 aaatgaaggg ctttgttgtc agaaaacatt taaaatcctt atgttagaat ttttagatga
      421 caattatttt ttgatatgaa aaaaataaca atcttttttt gtttataatg agagacagat
      481 cacataatta ttttttacgt ttaaatattt caggaaaagt atatggagtc tttgattcac
      541 taccaatggg ttgaaacaaa tgagcaatta gaggccgtat gtgaacaggc cagaaaaaca
      601 aaagtagttg ccttagatac agagtttatc cgtacacgta cctattatcc gatattgggt
      661 ttaattcaac tctttgatgg ccaacaggtt agccttattg atccaaatac gatttctgac
      721 ttctcgcctt ttgtgtcatt attagccgat aaaaatatca taaaagtatt acatgcctgt
      781 agtgaggatt tagaagtttt tgagtatcaa tttaagcaat tgccagagcc gatgttggat
      841 actcaggtga tggcagcctt tgccggcatt ggtatttcga tggggtttgc aaaattggtt
      901 tcccactact taaatattga actagacaaa ggcgcttccc gaaccgattg gcttgccaga
      961 ccgctaagcg ctgaacaatt acaatatgcg gcagctgatg tttggtattt attgccagtt
     1021 tatgagaaat tggcacaagc attaacaaaa agcacttggc aaagtgctgt agcagaggag
     1081 tgtgtcgctt tgttgaagaa agcgaaacag cctatcaata aggaaaaagc ctataaaaat
     1141 attgccaatg cttggcgatt gaatcgggaa gagttagcca tattacaagt attagcgaaa
     1201 tggcggattg aagaagcaca aaagcgcaat cttgccttaa attttgtcgt gaaggaggca
     1261 agtttagttc aaatcgcaaa aagctgtcct aaacacactt cttcgctgtt agaatttatg
     1321 cacccaaatg aagtcagaat tcacggaaaa aaaatactat ggttggttga gcaaggaaaa
     1381 gcaatagaat cgaatgatta tcctgaactc attcagcgct tggtggatga aaagggttat
     1441 aaatattata tgcaagcgat gttgcaaaaa ttattagaaa ttcaaccgct tgatatcgta
     1501 ccggagttgg ttgcaagtaa acgtcaatta aatcagctat ttaaatggta tattgatgga
     1561 aaaccatcag aaaaaattcc tgaattatta atcggttggc gaaaaccttt tggtgaacag
     1621 ctgctatcgg ttttatctta aatttcattc gggttacata gactatgtac agtataattg
     1681 cctaaaaagg agtttatatg gaaaataaaa aaccaccaaa agcattagat gcgattgatt
     1741 taaaaatctt aaacgagcta caacgtaacg ggaaaatttc taatattgaa ttatcaaagc
     1801 gagttgggct ttctccaacg ccctgcttag aacgggtaaa acggctagaa aaaaataacg
     1861 taattatggg ctataaagct ttacttaacc ctgaattact tgatgcccct ttactggtga
     1921 ttgttgaaat tactttggtg cgtggtaagc ctgatgtatt tgaagaattt aataaagcga
     1981 ttcaacaact tgatgaaatt caagaatgcc acctggtatc gggggatttt gattatctgt
     2041 taaaaacccg tgtggcggat atggtatcgt accgtaagtt attaggcaca acattgcttc
     2101 gtttgcccgg cgtaaatgac actcgaacct atgtggtgat ggaagaagta aaacaaacca
     2161 actatttgtt gttaaagtaa ggagaattgg gtgattgaac aacttaaacc acacctaaaa
     2221 ggcaaagaga acttgatcaa acttatgctt ttgttgaccg gctttttggg cgtttatttg
     2281 ctaattgctt gggcaagtta tagtccatta gataatgctt ggtctgttgc aagcagcatt
     2341 acacaaacaa ccttgaataa aacaggtcac tttggtgctt ggagtattga tatgctctat
     2401 gcaatgtttg gtaaggttgc agttttagta cctattgcat taattatttt ctcaatttat
     2461 agcttgggaa tgggtttaag tgtcgatatg aaatggaaaa ctgtcttcat tcgcctagcc
     2521 agtttcttat tattgatggt cggtcttgcc gggttattta gcgtgctttt ttcaaactca
     2581 gcttattatc tgtcgggtgg ctttgtcggt ggagtttggg agacaatatt atctgatact
     2641 atcggacaat ttggtgcctt attgattgca atgcttttta ctatagctgg cttatatttt
     2701 tgctctgttc aatcattatt gccaatatta acgcaatttt atgagtggat aatggcaaaa
     2761 tcagaagaga gcaaaacagc tgaatctctt cagcatttgc aaaatactga gcaaaaacaa
     2821 ccgcttgtaa ctgaaaatga agcggtggaa gttgtagatg accataaacc gcaatttacc
     2881 gatatctccg cctttaaacg cccaaacatt agtggtttaa gacactcgga aaatgtagag
     2941 atagataacc gagaaatgta tgaaattgaa agagatcttg agctaccgac tattaatatt
     3001 ttaatacagc ctgatatggt taaccaagtt gcggaacaag ctacatcggg ctatgagtta
     3061 ccaaacgaag aatccaatgt attgcaaatg ccgaccattc gcttaaatac cgctatcgaa
     3121 aacgaggtgg atacaatcga aaacagttca tcattggaag acctgaaaat agcagaatct
     3181 atgttacctc aaattcagct tgataatgat attgaagata ctgaagaagc tattgacgat
     3241 gtgcgtgaaa ttacaccgga atttatggta acgccgccca aggcgatgaa agatgtgtct
     3301 gagcagccag cctatccaaa aggctatggt gatacgttaa ttcacccgtt gttacaacga
     3361 aaagtgataa ccgaaaaacc gacaacacca ttgccaacgc tagatttgtt ggataaagca
     3421 ccgattcaaa gccagcaaat taccgaacag gaaatacgag aaacctctgt gcggctagaa
     3481 gcagaattag caaactttgg cgtaaaagct accgttgaag atgtattggt cggtccggta
     3541 gtgacccgtt atgaaatcca accggctgcc ggggtaaaag cctccaaaat taccaattta
     3601 gcgagcgata tagctcgtgg cttaatgttt aaagcgatta ggattactga agttatccca
     3661 aacaaacctt atatgggtat tgaaactccg aataaacacc gtgaaacagt gtggttgcgt
     3721 gatgtgctag acagcgatga attccgcaat accactgcaa cattgccaat ggcactaggt
     3781 aaagacatca gtggtgagcc tgtggtggtc gatatggcga aaatgccaca cttactggtg
     3841 gcagggcaga ccggtggggg taaatcggtt ggggtgaata cgatgatttt aagtctattg
     3901 ttcaaactta caccagaaca agtccgcttt attatgattg accctaaagt ggtggagctt
     3961 tccatttata acgatattcc tcacttatta acgccggtag taaccgatat gaaaaaagcc
     4021 gccaacgcac tacgctgggc ggtggaggaa atggaacgcc gttatttgct ggtaagccat
     4081 ttacaggtgc gtaatattga aggctataat gcgaaaatcg accaagcggc agcgatgaat
     4141 ttgccgattc cagatccaac gtggcgaccg ggcgattcaa tggatagcct tccgcctccg
     4201 ctacaaaaat taagctatat tgtgctgatt gtcgatgagt ttgcggattt aatgatgtct
     4261 gccggtaaag aagtggaaga gtacattatg cgaattgctc aaaaagcccg agcggtggga
     4321 attcacttaa ttcttgctac ccaacgtccg tcaaccgatg tgattaccgg cgtgattaaa
     4381 gccaatattc caagccggat tgcgtttaca gtagcgagcc aaattgactc aagaactatt
     4441 ttagattcag gtggagcaga ggcattatta gggcgaggcg atatgctcta ttcaggagct
     4501 ggcagcccgg atattattcg gatacacggt gcgtttatga aagacgaaga agtgcaacgc
     4561 gtggcggata attggcgggc aagaggaaaa ccaaactact tggaaagcat tgtggaatcc
     4621 cgctcggaag aggctgatgg caaaaacgat ggcggaacgg gcgatttaga tccgctattt
     4681 gacgaggttg ttgaatatat cacggaaacc ggttcggtct ctatcagtaa tattcaacgc
     4741 cgcttctcgc ttggttttaa ccgtgcggct agaattgtgg atcaaatgga agctcaaggc
     4801 attgtttctg agccacttaa aggtggcaaa cgagaagtgc tggcgaggta aaacacatac
     4861 aagcggttag atttctgtga aattttgcaa attttgacaa aaatctaacc gattgtattt
     4921 atctaatgct aaataagcat agtataaggc tagtctttgg gctaaaatta acgcaatttt
     4981 tacatctttt aaggttgaat atatgcgatt agatgatatt aaagaaagta aaaatgttga
     5041 agacagaaga ttcaacaatg tgggtagaac taaggttggt agagggaaag gtggaatatt
     5101 aacctttatt atcgtgcttg ttggagctta ttatggtgtt gacttaacag gtttaatggg
     5161 cggtggcgta gaatatgagg agcagacatc agcattaaat gaaagtgaag aaaaacagct
     5221 aagaacctta tccgctaaag tattaacgac cacagaaaat atttggactt cctacttcca
     5281 gcaaaatggt ctaacttata aagagccgtc tttggtgctt taccgtgggg caattcaaac
     5341 tgcctgtggc accggtcaat ctgcaatggg tcctttctat tgccctgtcg atcaaaaggt
     5401 ttatttagat ttatcattct atgatgatat gcgtaaaaaa ttaagagcat cgggcgattt
     5461 tgccttctct tacgtgattg cacacgaggt tggccaccac gtccaaaatt tattgggcat
     5521 aacaagcaaa acccaacggg ctcaaatgaa agcctcatct aaagccgaag caaaccaaat
     5581 ttcagtcaat gtagaattgc aagctgactg ttttgccggc gtttggggac accaactcgc
     5641 aaaagccaac cgattagagg aaggcgatgt ggaagaagcc tttaatgctg cccaagcagt
     5701 tggagatgac cgattacagc aacaaagtcg tggctatgtc gtgccggata gcttcaccca
     5761 cggctcatct gcacagcgtt tggcttggtt tagaaaaggt t
//
LOCUS       DANLYI010000151         5797 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.152, whole genome shotgun sequence.
ACCESSION   DANLYI010000151 DANLYI010000000
VERSION     DANLYI010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 5797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5797
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.152"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            complement(<1..707)
                     /locus_tag="PXD81_001938"
     CDS             complement(<1..707)
                     /locus_tag="PXD81_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005712160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDL5537942.1"
                     /translation="MSIYRRKANGPWWVDITTPNGKRIKRSTRTLVKREAQQYHDKLK
                     QEMWAEDKLEEKRKYIFEDALLHYVRAAEELKDKATKKRHAEYWLSKFAGRELSSLTA
                     QEIILNIPKKNANTKKPLSHSTQNKYVKSLSRVLNIAHKLGMLDAVPHIPKKKEPPIR
                     VRWITKEQAKQLIDKLSSDWMKSICKFALMTGARRTEILTMTWDKIDLVRKVAIVTND
                     VAKSGKARSLLLNQEAI"
     gene            complement(611..934)
                     /locus_tag="PXD81_001939"
     CDS             complement(611..934)
                     /locus_tag="PXD81_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005822951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL5537943.1"
                     /translation="MEHLTINQVAKELNCHPNTVRQHLADWGFFKMVGSEVWRINRED
                     LERAKKRAGIDPIRKRSDNDRGLSLSVEENEKCRSTDEKQTVRGGLISQPQTARELRE
                     ALARL"
     gene            complement(1015..1182)
                     /locus_tag="PXD81_001940"
     CDS             complement(1015..1182)
                     /locus_tag="PXD81_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HDL5537944.1"
                     /translation="MQFTYGSICSGIEATSIAWHGLGKPLWFSEIEPFPCAVLAHRFP
                     NVPNGNGMAVP"
     gene            complement(1190..1564)
                     /locus_tag="PXD81_001941"
     CDS             complement(1190..1564)
                     /locus_tag="PXD81_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5537945.1"
                     /translation="MHIEDKIAWWLANGETGVSSKTMAFYLGYGIRPKIEGYPHDVSD
                     FRRCFLLLETVPFLRNRIEKMAELGKVWAALAKEWHTLEALYNEEECQIRCPKTYAKL
                     REILEANEENVVRIGNLSISIG"
     gene            1714..1941
                     /locus_tag="PXD81_001942"
                     /pseudo
     CDS             1714..1941
                     /locus_tag="PXD81_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005609911.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
     gene            1966..2361
                     /locus_tag="PXD81_001943"
     CDS             1966..2361
                     /locus_tag="PXD81_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL5537946.1"
                     /translation="MKIHTQIRKEILSLLEAKLTDIEHFYNGQPNFIDIDEQQLAISV
                     YLDEINRQELTLCDEQWTAQLNITIYLKSVDEAEDELDEWAEKIREVVETYSAFEHLE
                     GISLSQYQYEQDQNQRTWHSATLIFDVEY"
     gene            2393..3034
                     /locus_tag="PXD81_001944"
     CDS             2393..3034
                     /locus_tag="PXD81_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5537947.1"
                     /translation="MAKTTKVQGTKFRIGIGREAQKAITAITLATATLTIATSGYKKG
                     DAIEITGCGQLDGIYPVLSVTGDQVKLCEEVNWTGKDLPANYTKAKAALVQYSDQFCA
                     VKNIEKSDDTLSTEDVTTVCSEGTETEPGEIEFGSIKLSFFHKPSTEMQTRLRKLFYD
                     KSTFAYKLELPDNHGTTYGEGFIEAGNGFSGEVKGKYEGSVSIKPSKRDYLLV"
     gene            3117..3518
                     /locus_tag="PXD81_001945"
     CDS             3117..3518
                     /locus_tag="PXD81_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5537948.1"
                     /translation="MTLREKLLANKPKLQSIEINGETYYLREATVGDMNKQIFETRSW
                     LIQQAEQENVELPAEDDETFDEALNRFGEKYRLAQSVAYRLCDENGALLFNPLNIDDL
                     NAIAELDSKVIIDFNQAVSAPKDSASEESSK"
     gene            3563..3793
                     /locus_tag="PXD81_001946"
     CDS             3563..3793
                     /locus_tag="PXD81_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4035 domain-containing protein"
                     /protein_id="HDL5537949.1"
                     /translation="MPERHFAEYQLFYQEQPFGLWREDYRAAQLAHLTAMINRDPKGK
                     APELSEFMPFFNRADEVEANEDDGVADYLAKR"
     gene            3861..4127
                     /locus_tag="PXD81_001947"
     CDS             3861..4127
                     /locus_tag="PXD81_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HDL5537950.1"
                     /translation="MATINDAFSFRTNTEIKNTAFDVIKNYGMTPSQVFNMFLTEIAK
                     TKTIPLSLNYQPNLETKLAMQEAKSGKNEVYASLEAFHKAMLAE"
     gene            4131..4400
                     /locus_tag="PXD81_001948"
     CDS             4131..4400
                     /locus_tag="PXD81_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin, RelE/StbE family"
                     /protein_id="HDL5537951.1"
                     /translation="MLQISPTNAYKRDFKKIAAELVGSSEYVEVMYCLINQLPLAEKY
                     RDHPLQGEWQGFRDCHIKPDLVLIYAVEDNLLRLVRLGSHAELFG"
     gene            4475..4738
                     /locus_tag="PXD81_001949"
     CDS             4475..4738
                     /locus_tag="PXD81_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5537952.1"
                     /translation="MKNVLLFIVHFFLIAMLSIIPILIILGAIERDPYYTAWIFLSVF
                     LGIGALLYFATIVSKLINKGKTQTLEEKRYAEYWNNVKVNIPK"
     gene            4798..5508
                     /locus_tag="PXD81_001950"
     CDS             4798..5508
                     /locus_tag="PXD81_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HDL5537953.1"
                     /translation="MSGLGKLTVTLELENAKFQSAMTKSDYEAQKFAKNFIQNMDRAR
                     NHAKEFADRSTQYLKNIEQAAKNLNKNSEFSFLSTIGGHYQSMSSGILSAAKAYTDIQ
                     NKMKLVSGSSAEAAKRLTDVFDIATKTSQSTEAVSGVYQTFAQNAQSLGLAQKDVAEL
                     TKTVSQAVAASGASSSAASNALTQFGQSLLMGKMKAQEFNSLITQTPTIIQAMAKGLG
                     MTMAEFKAAVYRAKSLLG"
     gene            5486..5776
                     /locus_tag="PXD81_001951"
                     /pseudo
     CDS             5486..5776
                     /locus_tag="PXD81_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484718.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
BASE COUNT         1814 a         1182 c         1121 g         1680 t
ORIGIN      
        1 ttaattgcct cttgattcag taatagcgat cgtgccttac ctgatttagc gacatcatta
       61 gtcacgattg ctacctttct tacaagatct atcttatccc aagtcattgt caaaatttct
      121 gtccgtctgg ctccagtcat caaagcaaac ttacagatag atttcatcca gtctgaactt
      181 aatttatcta ttagctgttt tgcttgttct ttggtgatcc agcgtacacg aattggcggc
      241 tctttctttt tcggtatgtg tgggactgca tccaacatac caagtttatg agcgatattt
      301 aatactctcg ataaagattt cacatactta ttttgcgtac tatgagatag aggctttttc
      361 gtatttgcat tctttttcgg gatatttagg ataatctcct gagctgttaa agagctgagt
      421 tcacgaccgg caaatttgga taaccagtat tcagcatgtc gcttttttgt ggctttatct
      481 tttaattctt cggccgcacg aacgtaatgg agcaaagcat cttcaaagat atattttctc
      541 ttttcctcga gtttatcctc tgcccacatt tcttgcttga gtttatcgtg atactgctga
      601 gcttcacgtt ttacaagcgt gcgagtgctt ctcttaattc tcttgccgtt tggggttgtg
      661 atatcaaccc accacggacc gtttgctttt cgtctgtaga tcgacatttt tcattctcct
      721 cgaccgacag agataaccct cggtcattat cagatcgttt tcttattggg tcaataccgg
      781 ctctcttttt tgctctttcc aaatcttctc ggttgatccg ccaaacttcc gagcctacca
      841 ttttaaaaaa tccccaatcc gccaaatgtt ggcggactgt atttggatgg caatttaatt
      901 cttttgccac ttgattaata gtaagatgtt ccattcatta ctttctccaa aataaatccc
      961 tctaaacgag ggattgtact aaataatcgt taagtctttg ccttatccac tgcatcaagg
     1021 cacagccata ccgtttccat ttggcacatt ggggaaacgg tgagctaata ccgcacaggg
     1081 gaatggctca atttcagaga accacaaagg cttacctaaa ccgtgccacg ctatacttgt
     1141 agcttcgata ccgctacaaa ttgagccgta ggtgaattgc attttttacc tatcctatcg
     1201 aaatactcaa attcccaata cggacaacat tttcttcgtt ggcttctagg atttctctca
     1261 acttagcata ggttttgggg catcttattt gacattcctc ttcgttgtag agagcctcta
     1321 atgtgtgcca ttcttttgca agtgcagccc aaactttccc aagttcagcc attttttcaa
     1381 tcctatttcg caaaaaaggg actgtctcca acaacagaaa gcaacgcctg aaatccgata
     1441 catcgtgtgg gtagccttct atttttggtc ttattccgta accaaggtaa aaagccattg
     1501 ttttactgct tacccctgtc tctccattgg caagccacca agcgattttg tcttctatgt
     1561 gcatttttcc tccaaaaatt aaccgcttgc ggaacaagtt ctacaagcgg ttgtcaagtg
     1621 ttcatttgtt caatgaacag ttcattgtga ataacaaaac cgcttgaatt acaagcggtc
     1681 tattttgttg cgtaaattta ctgttagcgt tgagccataa ccgactacga ccgctggccc
     1741 gaagatattg acggcgaggg ggacggcttc tcgcttgcct ccaaacggac gaccactttt
     1801 atgtcagccg gaatgacttt agtggagagt tcgcctggta aagatattgt cgatctcaaa
     1861 catatcccga aaagtagcca cgaagcccca ccgactaccg gtattttaag cctctacaat
     1921 cgaggcgacc gcagacgctt tagcgaaaca gttgataggt aagctatgaa aatccatacc
     1981 caaatccgaa aagaaatcct ttcattatta gaggcaaaat taacggacat cgagcatttt
     2041 tataacgggc aaccgaattt cattgatatt gacgaacagc aacttgccat ctctgtttat
     2101 cttgatgaga ttaaccgtca ggagcttacc ttatgcgatg aacagtggac agctcaactt
     2161 aacatcacga tttatctaaa atcagttgat gaggcagaag atgaattaga tgagtgggcc
     2221 gaaaaaatcc gtgaagtggt tgaaacatat tctgcgtttg aacatttaga gggcatcagc
     2281 ctttctcaat atcaatacga acaagatcaa aatcaaagaa cttggcactc agccacattg
     2341 atttttgatg ttgaatacta acaagcaaat aatccttata ggagaatcta caatggcaaa
     2401 aacaacaaaa gtccaaggca caaaatttag aattggcatt ggacgtgaag cccaaaaggc
     2461 aattacggct atcactttag ctacagcaac gcttactatt gccacatcag gctataaaaa
     2521 aggcgatgca atcgaaatta ccggttgtgg tcagttagac ggcatttatc ctgtattatc
     2581 tgtaacaggc gatcaagtca aattgtgcga agaagtaaac tggacgggta aagatttacc
     2641 ggctaattac accaaagcca aagcagcttt agtacaatac tcagaccaat tctgtgcggt
     2701 aaaaaacatt gaaaaatctg atgatacgtt aagtaccgaa gatgttacaa cggtctgctc
     2761 ggaaggtaca gaaaccgaac ccggagaaat tgaatttggc tcaattaaac tgagcttttt
     2821 tcacaagcca agtacggaaa tgcaaactcg cttacgcaaa ttgttttatg acaaatccac
     2881 ctttgcgtac aaattagaat tacctgataa ccacggcaca acctatggcg aaggtttcat
     2941 tgaggcgggt aacggcttta gtggcgaagt taaaggcaaa tatgaaggct cggtatcaat
     3001 taaaccaagc aaacgtgatt atttgctagt gtaattacaa aatattgctc caaatcgacc
     3061 gcttgtaata ttgcaagcgg tcttttttat cctaaatttt acaaaaggaa tcgacaatga
     3121 cactccgtga aaaactcctc gccaacaaac caaaattaca atctatcgaa atcaacggtg
     3181 agacctacta cctgcgtgaa gccactgttg gcgatatgaa taagcagatt tttgaaaccc
     3241 gaagctggct cattcaacaa gctgaacaag aaaatgttga attaccggca gaagatgatg
     3301 aaacctttga cgaagctctc aaccgttttg gcgaaaaata ccgccttgct caatcggttg
     3361 cctaccgttt atgtgacgaa aacggtgcat tactgtttaa cccacttaac attgacgatc
     3421 tcaatgcgat tgccgaatta gacagtaaag tgattatcga ctttaaccaa gccgtgtccg
     3481 ccccaaaaga ctcagcgagc gaagaaagtt ccaaataacc ctttcgctcg cactgggtaa
     3541 aacccttgaa gaaatcgaac aaatgcctga acgccacttt gccgaatatc agctttttta
     3601 tcaagagcaa ccttttgggc tttggcgtga ggactatcgt gctgctcaac ttgcccactt
     3661 aaccgcaatg atcaaccgtg acccaaaagg caaagcccct gaactctcgg aatttatgcc
     3721 gttttttaat cgggctgatg aagtagaagc aaatgaagat gatggcgttg cggattattt
     3781 ggcgaaaagg taaatctctt gtatttcatt gtaattctgt ctataattgg tgtaatacat
     3841 agaaatacag gagagataaa atggcgacta tcaatgatgc tttcagtttt agaacaaata
     3901 ccgaaataaa aaataccgca tttgatgtaa ttaaaaacta tggaatgaca ccctctcagg
     3961 tgtttaatat gtttttaacc gagattgcaa aaacgaaaac tattccgtta agtttgaatt
     4021 atcaacccaa tcttgaaaca aaattggcaa tgcaagaagc aaaatcaggt aaaaatgaag
     4081 tttatgcctc acttgaagca tttcataaag caatgttagc ggagtaaata atgctacaaa
     4141 tttcgccgac aaacgcatat aaaagagact ttaaaaagat tgcagccgaa ttagtcggta
     4201 gttcggaata tgtggaagta atgtattgcc taataaacca attaccattg gcggaaaaat
     4261 atagagatca cccactacaa ggtgaatggc aaggctttag agattgccat attaagcctg
     4321 atttagtgtt gatttacgct gttgaagata atctgctccg ccttgttcgt ttaggatcgc
     4381 acgctgaatt atttggataa tttctgtaaa aactaaccgc ttgcgatttt gtgctgtggg
     4441 cggtatagtt ggaatattaa caaaggaggg aaaaatgaaa aatgtacttc tatttatcgt
     4501 acactttttc ttaatcgcaa tgctctcaat tatcccaata ttaattattc ttggagcaat
     4561 cgaaagagat ccttactata ctgcgtggat tttccttagc gtatttttag gcattggagc
     4621 attgttatat tttgcaacca ttgtttctaa gttaattaac aagggaaaaa cacaaacatt
     4681 agaagaaaaa cgatatgcag aatattggaa taatgtaaaa gtcaatattc ccaaatagtc
     4741 taactgataa atgaccaaaa gctcgcttta tgcgggcttt tttattggag aaaaacaatg
     4801 agtggcttag gaaaattaac cgttacctta gagcttgaaa atgcaaaatt tcagtccgca
     4861 atgacgaaat ctgattatga ggcacaaaaa tttgccaaga actttatcca aaatatggat
     4921 agagctagaa atcacgccaa agaatttgcc gatcgctcaa cccaatattt aaaaaacatt
     4981 gagcaagcgg caaagaacct caataaaaat tctgaatttt catttttatc gactattggt
     5041 ggacattatc aatcaatgtc tagtggcata ttgagtgcgg caaaagccta tactgatatt
     5101 caaaataaaa tgaaattagt aagcggtagt tcagcagagg cagctaaacg attaactgat
     5161 gtgtttgata ttgcgactaa aacaagccaa agtacagagg ctgtttcagg tgtctatcaa
     5221 acatttgctc aaaatgccca atctctcgga ctggctcaaa aagatgtagc agaattaact
     5281 aaaacggtct ctcaagccgt tgctgcaagc ggcgcaagca gttctgcagc aagtaatgcc
     5341 ctaacccaat ttggtcaatc acttttaatg ggcaaaatga aagcccaaga atttaactcg
     5401 ctcattactc aaaccccaac cattattcaa gcaatggcaa aaggtttggg aatgacaatg
     5461 gcagaattta aagcagcggt ataccgggca aaatctttac ttggataaca ttcaagggca
     5521 aggttttgag agagtggata caccccaaat cggcgatgtg attttaatac aggtcggttc
     5581 tgatgtgccg aatcacgcag cgatttatat tggtgaacaa atggtaatcc accatagccc
     5641 gaaccgatta tctaagcgtg atttatatga cggttattgg ttacgccata cccacagtat
     5701 ttggcggcat aaactggcgg atagattgga ttttgacggc atatttaatg atataggaga
     5761 acaatatgaa atttagtaaa tatcaattat tagttaa
//
LOCUS       DANLYI010000152         5737 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.153, whole genome shotgun sequence.
ACCESSION   DANLYI010000152 DANLYI010000000
VERSION     DANLYI010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 5737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5737
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.153"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            <1..113
                     /locus_tag="PXD81_001952"
     CDS             <1..113
                     /locus_tag="PXD81_001952"
                     /inference="COORDINATES: protein motif:HMM:NF013463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HDL5537954.1"
                     /translation="SFNHPQFYQLISPNGELCIVPFQSRQHLDYLAQKHH"
     gene            144..2012
                     /locus_tag="PXD81_001953"
     CDS             144..2012
                     /locus_tag="PXD81_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HDL5537955.1"
                     /translation="MSGLVSPELIKTVALLATSVTIVPLFKRLGLGSVLGYLVAGCLI
                     GPSGIGLFQDPAAVVHMAELGVVMFLFIIGLEMHPELLWAMRKAIFGRGFLQVAICGT
                     LLTLAGIYLLGFSKEVAFIAGMGFTLSSTAIVMQVLEDKGISSTPKGQRIVATLLFED
                     LAIVPLLASVAFLAPNQVDAESSTNWSAIGLSLVAVLILVASGKWLMNPIFRVISKAK
                     VREMMTAAALLVVLGSALLMEISGLSMAMGAFLAGVMLSESSFRHQLEADIEPFRGLL
                     LGLFFMGVGMSLDLSLVLNNWLWLLVIVAVYVIGKGLGIFIAASITRLTLNESIVRTT
                     IMAHGGEFAFVLFSAAATAGILTPDTHATLTAAVIVSMLLSPLLILIKQGVRKKKAKN
                     ESTNMTGVELPEDLEANVLVIGFGRFNQIVCQALLAKGLSVSVIDSNTDHIRAAARFG
                     FKLYYGDGARLDVLRASGIANANCVIVGVGNPERTEKIVELIKSEYPLVPVFARTYDR
                     QSAVDLVKKHHVDYHIRETFESALALSQAAIERLGSSEEEAKQIISYVRRLDAERFNE
                     ELLHGFSAEVIRKYWVPTPWVNPQHEGSALNEETADILEELGETVEVIKVDITDKN"
     gene            complement(2172..3068)
                     /gene="malM"
                     /locus_tag="PXD81_001954"
     CDS             complement(2172..3068)
                     /gene="malM"
                     /locus_tag="PXD81_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HDL5537956.1"
                     /translation="MKKTLLSTAIFFAHLLVVSPSIANGSAGKSIHINSAELAQIQWQ
                     DVPFSQTVKTDLSEQHKQAFTTAFAGIASPVAAYRIPANQGTLEIEIESPVMDKNVFV
                     PTAVVLDSQFNVASTYPSSEFKLLEERGLKGSRLGAELNLTPAMNQDYIYLLIYTTQQ
                     DLAKTTMMPHPAKTYAKATGKQPPAINDIEVAHSLKGQIHINVTGSNGTKFIGLPTEI
                     FSSNKAATPAAKTAIPLPIKENPVAVKTAVDKDTEAYFNQAVMKAIKAKDINKAMNLV
                     NEAEKLGLTSPRQIFLKNVSSN"
     gene            complement(3149..4437)
                     /locus_tag="PXD81_001955"
                     /pseudo
     CDS             complement(3149..4437)
                     /locus_tag="PXD81_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248001.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin"
     gene            complement(4535..5653)
                     /gene="malK"
                     /locus_tag="PXD81_001956"
     CDS             complement(4535..5653)
                     /gene="malK"
                     /locus_tag="PXD81_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005552148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HDL5537957.1"
                     /translation="MANVSLRNVGKSYGDVHISKDINLEINEGEFVVFVGPSGCGKST
                     LLRMIAGLEDITTGELYIGGKLMNEVEPSKRGIGMVFQSYALYPHLDVAENMSFGLKL
                     AGVSKAERDQRVNQVAEILQLAHLLDRKPKALSGGQRQRVAIGRTLVSQPEVFLLDEP
                     LSNLDAALRVQMRVEISKLHKKLNRTMIYVTHDQVEAMTLADKIVVLNAGGIAQVGKP
                     LELYHYPANRFVAGFIGSPKMNFLPVKVIAVEPERVKIELPDANHHNFWIPVSGEGVN
                     VGDNLSLGIRPEHLVPSDQTQVTLHGIVQVVELLGNETQIHLEIPEIKQPTLIYRQND
                     VVLVNEGDDLNIGILPERCHLFKEDGTACKRLFVEKGV"
BASE COUNT         1579 a         1202 c         1129 g         1827 t
ORIGIN      
        1 gaagctttaa tcatccacaa ttttatcaac ttatttcacc aaacggtgag ttatgtattg
       61 tgccgtttca aagccgccag catttagatt atttagcaca aaaacatcat taatcttatt
      121 tatattatac aggtgggaat tctatgtcag gattagttag tccggaatta attaaaacag
      181 tcgcattact tgcaaccagc gtcactattg taccgctatt taaacgcctt ggtttaggaa
      241 gcgtattggg ttatttagtc gcaggatgtt taatcgggcc atcgggcatt ggattgttcc
      301 aagaccctgc cgctgttgtg catatggcag aacttggcgt ggttatgttt ttgtttatta
      361 ttggtttgga aatgcacccg gaactgcttt gggcgatgcg taaagccatc ttcgggcgag
      421 gtttcttaca agttgcgatt tgcggtactt tattaacctt agccggcatt tacttattag
      481 gtttttccaa agaagtggca tttattgccg gtatgggttt tacgctatct tctactgcca
      541 ttgtaatgca agtgctggaa gataaaggga taagttccac gccaaaaggg caacgtattg
      601 ttgcaacttt actatttgaa gatttagcga ttgtgccatt attagcctcg gttgcctttc
      661 ttgctcctaa ccaagtagat gctgaatcca gtactaattg gtctgctatt ggtcttagcc
      721 tagttgccgt tcttattttg gttgcgagtg gaaaatggct gatgaaccct attttccgtg
      781 ttatttctaa agccaaagta cgagaaatga tgactgccgc tgccttactt gtggtattag
      841 gctccgctct tttaatggaa atcagtgggc tttcaatggc aatgggggca ttccttgccg
      901 gagtaatgct ttcggagtct tctttccgcc atcaactaga agctgatatt gaacccttcc
      961 gtggtttact tttaggctta ttctttatgg gcgtaggaat gtcattagac ctcagcctcg
     1021 tcctcaataa ttggctatgg ttattggtga ttgtagcagt ttatgttatc ggtaaaggcc
     1081 ttggaatctt cattgctgcc tctatcaccc gattaacact gaatgaatct attgtgcgaa
     1141 ctaccattat ggctcacggt ggtgaatttg cctttgtgct attctcagcc gccgcaaccg
     1201 ctgggatttt aaccccagat actcacgcta ccttaaccgc tgcggtaatt gtctcaatgt
     1261 tgctttcccc acttctcatt ttaatcaagc aaggagtgag aaagaaaaaa gcgaaaaatg
     1321 aatccactaa tatgacaggg gttgaattac cggaagattt agaagccaat gtattagtca
     1381 tcggttttgg acgctttaac caaatagtct gccaagcctt attagccaaa ggactaagtg
     1441 tatcggttat tgattcaaat actgatcata ttcgtgctgc tgctcgtttt ggttttaaac
     1501 tttactatgg tgatggagcg agactagatg tacttagggc atctggcatc gccaatgcaa
     1561 actgcgtaat tgttggcgta ggcaatccgg aaagaacgga gaaaatcgta gagctgatta
     1621 aatctgaata ccccttagta cctgtatttg cgagaaccta tgatagacaa agtgcggttg
     1681 atttagtcaa aaagcaccac gttgattatc acattcgaga aacctttgaa tcagccctcg
     1741 cattaagcca agccgccatt gaacgcttag gttctagcga ggaagaggca aaacaaatta
     1801 tcagctatgt tcgccgatta gatgcagaac gctttaatga ggaattatta cacggtttct
     1861 ccgcagaagt tatccgcaaa tattgggtac caacaccttg ggtaaatcca caacacgaag
     1921 gtagtgcact taatgaagaa acggctgata ttttagaaga gcttggtgag acagtagaag
     1981 ttataaaagt ggatatcact gataaaaatt aaagaattta tactttgaat ccttattcaa
     2041 gtataagcct tcctatacat atcgcataat cactcgtaaa aaagcaccct atcggcataa
     2101 tctgataggg tgttaatggt aataaaccat cgcacgatga caaggagaat atctatgaaa
     2161 aaaacaataa atcaatttga acttacattt tttaagaaaa tttggcgtgg agaagtcagc
     2221 cctaattttt ctgcctcgtt aaccaaattc atcgctttat taatatcttt tgctttaatc
     2281 gctttcatta ccgcctgatt aaaataggct tccgtatctt tatctactgc tgtttttact
     2341 gcaactggat tttcttttat cggcaacggt atagccgttt tagctgccgg cgttgctgct
     2401 ttgtttgagc tgaaaatttc agtcggtaat ccaataaatt tagtgccgtt cgagccggta
     2461 acgttgatgt gaatttgccc ttttaaactg tgtgctactt caatatcgtt aattgctggt
     2521 ggttgtttac cggttgcttt tgcataggtt tttgccggat gcggcatcat cgtggttttg
     2581 gctaaatctt gctgcgtggt ataaatgagc aagtaaatat aatcttggtt cattgccggc
     2641 gttaaattaa gttctgcccc taaacggcta cctttgagtc ctcgctcttc caataattta
     2701 aactcagaag aggggtaagt tgatgccaca ttaaattgac tatccaacac gactgccgtg
     2761 ggtacaaaaa catttttgtc catcaccggg ctttcgattt caatttccaa tgtgccttga
     2821 tttgccggaa tacgataagc tgcaaccggg ctggcaatac cggcaaacgc tgtggtaaat
     2881 gcttgtttgt gttgctcgct tagatctgtt tttacggttt gtgaaaatgg cacatcttgc
     2941 cattgaattt gagctaattc agccgagtta atatgaatag acttacctgc cgagccattt
     3001 gctatactcg gagacaccac caaaagatgg gcgaagaata ttgctgtcga aagaagcgtt
     3061 tttttcatat taacccctaa aaatcgtgaa ataggataaa gtgctgaaat agtgggtagg
     3121 taatcctacc cacacaaagg tttgatgatt accaccaagc ttcaaattga acaccggtga
     3181 taaactcgcc gtctttggct ttatagccgg catcggtacg agctttcgtg ttgaatttat
     3241 cattccaacg tgcataagta ccaaacacac ggattgcagg gcgagcccaa atgctattac
     3301 cagcctgcca ttgttgtgca acggtgtatt ttaccaagtc atttttcttg cctgttgctt
     3361 gatctttaat gcgatcataa ccaacttcta ataatgtact cattgtatca ttccatttgt
     3421 acattgggcg aatacctgct gagtaccaag ttttaccttg tttattatct agttttgttt
     3481 tttcgtagat taaggcatac attacttcaa ctttatcact cgcttgcact accccttggt
     3541 tgattaaacg aagcatatta cctttattat ctgctttcga accctgtgcg tgcccattat
     3601 tccaagaagt catagagtcg gttgcatatt gtgcagtaaa tttattgaaa ccaccaaaga
     3661 agttaccttg agtgtattcc gctgttacca tataaccgtt tttggttgct cctttttcaa
     3721 gggctgcacc tttcttagca tgtgcattac cgtaatcaaa gcctaactct aacgaaccat
     3781 ctttccaaag ctcaataccg gctaaccgaa catcaaaaat atcgttgtaa acatcggctt
     3841 tattacgctg cttatctaat ttaattttac ctgtgctagg atctaattta taaactgtag
     3901 tatatccaaa agtattcgcc cctccacgct ctgtatcacg agtaaccgct aaagagagtt
     3961 ttccgaagcc taaatcaata ttttcaaccc ctgcacccgg acctgaaata tcccagtaat
     4021 agaagtcatt catatgtaca tcatggcgtt ggtaaaaacg tttaccagcc cataaggtgg
     4081 cacctggtaa actatctgca aagtttttga actgtacatt taattcacgt agtgctggac
     4141 ttgttggtgt ccaatcatta ttgtgaagcg taccgccgta agcaaggttt gtatcaaagt
     4201 agatagattt ctcaccactt ttgaataatt cctgacctaa tttcaattct gcataggtat
     4261 cagattcgtt acctaaacgg tattttgaac caccaccatt taccgtgaat gcggattgct
     4321 cccccccccc gatgtccaac cgatacctga acgtgcatat ccgtggaaat caaccgctga
     4381 tgcactggct gcgaacattg caccactgat tgcaactgct aggactgttt ttttcataat
     4441 agaacctctc ttaagatttg attttactta ggttaattat taattgatta gaagattgaa
     4501 aataccctct aaaatcagta ataaactgaa attgttatac ccctttttca acaaacaaac
     4561 gtttgcaagc tgtaccgtct tccttaaata aatggcagcg ttccggcaat ataccgatat
     4621 tcagatcgtc gccttcatta actaatacca catcgttttg acgataaatt aaggttggtt
     4681 gtttgatttc agggatttca aggtggattt gtgtttcatt tccgagcaat tccacaactt
     4741 gcacaatacc gtgtaaggtc acttgggttt gatccgaagg aactaaatgt tccgggcgga
     4801 tacctaaaga taagttatct cccacattca ccccttcgcc ggaaaccgga atccaaaaat
     4861 tatgatgatt cgcatcaggt aactcaattt ttacccgttc cggctctacc gcaatcactt
     4921 ttaccggtaa aaagttcatt ttaggcgagc cgataaaccc tgctacaaag cgatttgccg
     4981 ggtaatgata aagttctaac ggtttaccta cttgagcaat accgccggca tttaatacca
     5041 cgattttgtc cgctaatgtc attgcttcaa cttgatcgtg cgtaacataa atcattgtgc
     5101 ggtttagttt tttatgtaat ttggaaattt ctacccgcat ttgtacacgc aatgcggcat
     5161 caaggttgga aagtggttca tcgagtaaaa atacttccgg ttgggaaacc agtgttctac
     5221 caatagccac ccgttggcgt tgtccgccgg aaagtgcttt aggttttcga tctaataaat
     5281 gggcaagctg taagatttcg gcaacttggt taactcgttg atcccgctct gctttactta
     5341 cacctgccag ctttaagcca aaagacatat tttcggcaac gtctaaatgt ggatagagag
     5401 cataggattg gaacaccata ccgataccac gttttgaagg ctctacttca ttcatcagtt
     5461 ttccaccgat atagagttct cctgtcgtaa tatcttccaa acctgctatc attcgtaata
     5521 gcgtagattt gccacaacct gacggtccaa cgaaaacaac gaattctcct tcgttaattt
     5581 ctaaattaat atccttagag atatgaacat caccatacga ttttcccacg ttacgtaatg
     5641 atacattcgc cataactaaa cctcttttct gcttttgctt gttggtttta tttaggctga
     5701 atagtcgcct ttttatctaa agaagaaatc atcctaa
//
LOCUS       DANLYI010000153         5727 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.154, whole genome shotgun sequence.
ACCESSION   DANLYI010000153 DANLYI010000000
VERSION     DANLYI010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 5727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5727
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.154"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            complement(130..1533)
                     /locus_tag="PXD81_001957"
     CDS             complement(130..1533)
                     /locus_tag="PXD81_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HDL5537958.1"
                     /translation="MLTYTFNTQEGTALYEQLYRFLKNDIESNILIAGDKLPSKRAFA
                     KHLGISVMTVETAYQQLVAEGYVNTQPKKGFFVNALNLPEKKEAISVQLQKISSEQYE
                     KNKKWQADLTNRQTLAENFPFSVWTKLVREVLKTHQSELMERAESGGVWTLKFAIANH
                     LRAFRGMIVNPTQIIIGAGTEYLYGLLIQLLGLDKHYALTEPSYDKLRRIYHSYRLST
                     ATVSMPFSIDELNSKNVDVIHTSPSHHFPTGHVMPIAKRYELLAWVAQKAERYIVEDD
                     YDSEFRFVGQPIPALKSIDMLGRVIYMNTFSKTLSSTVRIAYMVLPPELLNRFQQELG
                     FYASTVSNFEQYILAEFIQQGYFEKHINRMRAYYQKKRDKLLKAFKQGNLAEKIAIKE
                     EHSGLHFIVQLNTTLTERQILQAAEEQGIKFVALSAYYQDKSQIQQNAFIIGYSNLKD
                     DQINYVAESLKKILDNE"
     gene            1744..2616
                     /gene="pdxS"
                     /locus_tag="PXD81_001958"
     CDS             1744..2616
                     /gene="pdxS"
                     /locus_tag="PXD81_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HDL5537959.1"
                     /translation="MTNRYDLNRQLAQMLKGGVIMDVTTPEQACIAEEAGACAVMALE
                     RIPADIRAAGGVSRMSDPKMIKSIQAAVSIPVMAKVRIGHVTEAQILQAIEIDYIDES
                     EVLSPADDTFHINKRKFEVPFVCGAKNLGEALRRINEGAAMIRSKGEPGTGNVVQAVH
                     HLRKMKQEIARLVSHTTDELYHVAKELQVPFDLVQYVHQYQKLPVVTFAAGGVATPAD
                     AALMMQLGAEGVFVGSGIFKSGDPAKRARAIVQAVTNYNDPKLLAELSEDLGEAMVGI
                     NEQEIELLMAARGV"
     gene            2616..3188
                     /gene="pdxT"
                     /locus_tag="PXD81_001959"
     CDS             2616..3188
                     /gene="pdxT"
                     /locus_tag="PXD81_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HDL5537960.1"
                     /translation="MKIAILALQGAFSEHTDKLQQLGVESMEIRQLADLNQDFDGLIL
                     PGGESTVQGKLLRELGLLEPLRQKILNGLPTLGTCAGLILLAENLVNDDKKHLATLPV
                     TVKRNAYGRQLGSFLVEEEVKYLGKIPLTFIRAPYIESTGKDVEILAKISGKIVGVKY
                     QNQIGISFHPEVNNDLSFHRYFVEMCRKNK"
     gene            3337..4287
                     /gene="tal"
                     /locus_tag="PXD81_001960"
     CDS             3337..4287
                     /gene="tal"
                     /locus_tag="PXD81_001960"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HDL5537961.1"
                     /translation="MSQLDTLSKMTVIVADTGDIEAIKLYKPKDATTNPSLILSASAL
                     PQYAPLIEEAINYAKAQSSDKDQQLVDAEDKLAVNIGLEILKLVPGRISTEVDARFSY
                     DTEKTIAKARKLIGLYNDAGISNDRILIKIASTWQGIKAAEQLEKEGINCNLTLLFSQ
                     AQARACAEAGVYLISPFVGRILDWYKAHEGKEYAPAEDPGVISVTNIYNYYKQHGYDT
                     IVMGASFRNVGEIIELAGCDRLTISPPLLKELQESDEKLVCKLDYKGEIKPRPEKMTE
                     AEFYWEHNQEPMAVEKLAEGIRKFAIDQEKLEAMLAEKLQ"
     gene            complement(4349..4622)
                     /locus_tag="PXD81_001961"
                     /pseudo
     CDS             complement(4349..4622)
                     /locus_tag="PXD81_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599987.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
     gene            complement(4653..5117)
                     /locus_tag="PXD81_001962"
     CDS             complement(4653..5117)
                     /locus_tag="PXD81_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014992099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HDL5537962.1"
                     /translation="MKKLALAVALMSSFALVGCANTDVFSGSVYSGEQAKEARSIAYG
                     TIVSVRDVKIQARSEGVIGTVGGGVLGGVAGNAIGGGTGQAIATAVGAVAGAVIGNQV
                     EQKASQVSSLEMVIRKDDGKEIVVVQKKEDGFVPGKRVRIVGSNSDLNVSLL"
     gene            complement(5138..5725)
                     /locus_tag="PXD81_001963"
     CDS             complement(5138..5725)
                     /locus_tag="PXD81_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005595649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDL5537963.1"
                     /translation="MAENAKFFEKMTACFGDLSQFEQKIRQIAEIAGIDLAAYEIDHL
                     AVRMNQTETAEQWKILLLDGATLLKESEVNGRPIGLFALIQAVEFCGQFVKIVELPFP
                     KNKVYLEEGWEHIELVFPMLDNESSEQWVSRALSHFRLADNSEIKLKISQPQVEGERL
                     PNPSIAISLRNVTFCNPYCLKLHPYDIKSVIMSEN"
BASE COUNT         1802 a         1113 c         1065 g         1747 t
ORIGIN      
        1 atgcattata cgcaaaacaa aatgccttgc aaggagaatt tggtaatttt ttgcaaaaat
       61 cactaaaata attaccactt gcgtgttttt aaggcaattt gctaaaaatt aaagcagttt
      121 tgatacttgc tattcgttgt ctaaaatttt tttcaggctc tctgctacat aattaatctg
      181 atcatctttt aaattagagt acccaattat aaaagcattt tgctgaattt gagatttatc
      241 ttgataataa gcagacaatg cgacaaattt tataccttgt tcttctgctg cctgcaaaat
      301 ttgcctttct gttaatgttg tgtttagctg tacaatgaaa tgcaagcctg aatgttcttc
      361 ttttattgca atcttttctg ctaaattgcc ttgcttgaaa gcttttagta atttatccct
      421 ctttttttga taatacgctc gcattcgatt gatatgcttt tcaaaatagc cttgttgaat
      481 aaattctgcc aaaatatatt gctcaaaatt ggaaacggta gaagcataaa accctaattc
      541 ctgctgaaaa cgattcagta attctggtgg caaaaccata taggcaattc gtacagttga
      601 agagagcgtt ttagaaaaag tattcatata gataactcgc ccgagcatat caatagattt
      661 caatgcagga attggttgtc ctacaaaacg aaattcgctg tcgtagtcgt cttctacaat
      721 atatctttca gctttttgtg caacccaagc taacaactca taacgtttgg caatcggcat
      781 tacgtgtcct gtcggaaaat ggtgtgatgg cgaggtatgt atcacgtcca cattctttga
      841 atttaactca tcgattgaaa atggcatcga tactgtggct gttgataagc gataactgtg
      901 ataaattctg cgcaatttat cgtaactggg ctcagttagt gcataatgtt tatctaagcc
      961 tagaagctga atgagcaaac cgtaaagata ctccgtaccc gctccaataa taatctgtgt
     1021 tggattaacg atcattcctc taaaggctcg aagatgattg gctatcgcaa attttaatgt
     1081 ccatacgccc ccactctcag cacgctccat tagctcactt tggtgtgttt tcagcacttc
     1141 gcgtactaat tttgtccaaa ccgaaaacgg aaaattttct gctaaagtct gtcgattagt
     1201 taaatctgcc tgccattttt tatttttctc atactgttct gaactgattt tttgtagctg
     1261 aactgatatg gcttcctttt tttctggtaa atttaaagca ttcacaaaaa agcctttttt
     1321 aggctgagta ttcacataac cttctgctac taactgttgg taagctgttt ccactgtcat
     1381 tactgaaatg cctaaatgct ttgcaaaagc ccgtttagac ggcagtttat cccctgctat
     1441 aagtatgttg ctttcaatgt cgttttttag aaaacggtaa agttgctcat agagagcggt
     1501 gccttcttgt gtgttaaaag tgtaggtaag cataatctga catgataaga aaaacataaa
     1561 atggtcattt taaacagttc agattaaatt acaatacatg ctgtttcctc actcaaattg
     1621 gctgagtgta tttaaattgg tagatgaaca ctcgcatcca gcgaatataa aaataaactt
     1681 aatgccgagc taatcgaaac tacgaaaagt tcaattattc aacaattaga aggaaaaagt
     1741 acaatgacta atcgctatga tttaaacaga caacttgctc aaatgttaaa aggcggtgta
     1801 attatggatg taactacccc tgagcaagcc tgcattgcgg aagaagctgg agcttgtgca
     1861 gtaatggcac tagagagaat tcctgcagat attcgtgcag ctggtggcgt ttctcgtatg
     1921 agtgatccca agatgataaa gagtatccaa gcagcggtat ccattcctgt aatggcgaaa
     1981 gtacgtattg gacacgttac agaagcacaa atcctgcaag cgatcgaaat tgactatatt
     2041 gacgaaagtg aggtgctttc tcctgcagat gataccttcc acattaacaa gcgtaaattt
     2101 gaagtgccat ttgtatgtgg agcaaaaaat ttaggcgaag ccctacgtcg tattaatgaa
     2161 ggcgctgcta tgatccgtag taagggcgag ccaggaacag gcaatgtggt gcaagcagta
     2221 catcatcttc gcaaaatgaa acaagaaatt gcccgcttgg tttcacatac cacagatgaa
     2281 ctttatcacg tagctaaaga gctccaagta ccatttgatt tagtacaata tgtacatcag
     2341 taccaaaaat tgcctgttgt cacctttgct gctggaggtg ttgcaacccc tgccgatgct
     2401 gcattgatga tgcagttagg tgcagaagga gtcttcgttg gctcaggtat tttcaagtca
     2461 ggcgatcctg ctaaacgagc gcgggccatt gtacaagctg ttaccaacta caacgatcct
     2521 aaattacttg cggagctttc tgaagattta ggtgaagcaa tggtaggaat taatgagcaa
     2581 gagattgaat tgctaatggc agcaagaggt gtctaatgaa aattgctatt cttgctctgc
     2641 aaggtgcatt tagcgagcat actgataaac tccagcaact aggggttgaa agtatggaaa
     2701 ttcgtcaact tgctgattta aaccaagatt ttgatggtct gattttacct ggtggcgaat
     2761 cgactgttca aggtaaatta ttacgtgaat taggcttgct tgagccgctt cgtcaaaaaa
     2821 ttttaaatgg cttgccaacg cttggaactt gtgcgggttt aattttgctt gcggaaaatt
     2881 tggtaaacga cgataaaaaa catttggcta ctttgcctgt aacagtgaaa cgtaatgctt
     2941 acggaaggca attaggctcg tttttagtag aagaagaagt taaatatctc ggtaaaattc
     3001 ctctcacatt cattcgtgcg ccttatatag aaagcactgg aaaagatgta gaaattttag
     3061 ccaagataag cggtaagatt gttggagtaa aataccaaaa tcagataggt atatccttcc
     3121 atcccgaagt gaataatgat ttaagttttc accgctattt tgtggaaatg tgccgaaaaa
     3181 ataagtaaaa gaaaaggggc tattgcctct tattttttat acttttttag aaaaagtagt
     3241 tttgaataaa aaaatactaa aactctattt cccttatgtt attattatag taacttttac
     3301 tactatttat ttcacaatca aaaaaggaat cagactatga gccaacttga caccttaagc
     3361 aaaatgaccg ttatcgttgc tgacactggt gatattgaag caatcaaact ttataagcca
     3421 aaagatgcaa caacaaaccc gtctttaatt ttaagtgcat cggcattacc gcaatatgct
     3481 ccattaattg aagaagcgat taattacgca aaagcacaaa gtagcgataa agatcaacag
     3541 ttagttgatg ctgaagataa attagcggta aatattggtt tagaaatctt aaaacttgta
     3601 cctggacgta tttctacaga agttgatgct cgtttttctt atgatactga aaaaactatc
     3661 gctaaagcac gtaaattaat tggattatat aacgatgcag gcatcagtaa tgatcgcatt
     3721 ttaattaaaa ttgcctctac ttggcaaggt ataaaggcag cagaacagtt agaaaaagaa
     3781 ggcattaact gtaacttaac gttattattc tctcaagctc aagctcgtgc gtgtgcagaa
     3841 gcaggggttt acttaatctc tccattcgtt ggtcgtattt tagactggta caaagctcac
     3901 gaaggtaaag aatatgctcc agctgaagat ccaggtgtaa tttcagtaac caatatatac
     3961 aattactaca aacaacacgg ttatgacact attgtaatgg gtgcaagttt ccgtaatgta
     4021 ggagagatca ttgagttagc tggttgcgac cgtttaacca tctcaccacc gttattaaaa
     4081 gagctacaag agagcgatga aaaattagtt tgcaagctag attacaaagg tgaaatcaag
     4141 cctcgtccag aaaaaatgac agaagcagaa ttctactggg agcataacca agagccaatg
     4201 gcagtagaaa aattagctga aggcattcgt aaatttgcga ttgaccaaga gaagttagaa
     4261 gcaatgcttg ctgaaaaact tcaatagaca acgcataaaa aaaccggcat taacgataat
     4321 taatgccggt tttaatttct ataaatgatt atttaaactc ataacgtcta atcgttttta
     4381 gatcgctatc ttcattttta atttcatcgg caagatgaac acgagcacca accgctgcat
     4441 gtgcatcata ctcacgagtt agagtcacag tttcaccatt atctcgttga actgtaagtt
     4501 caacaacatc gtttgatact gctttgctag ataaatgttg cattaccttt atcagaataa
     4561 acatctgcag tacaagctgc taacataagt gaagctaata cagctaaaga aagttttttc
     4621 attaataata tatcctctag gaaaaactaa aattaaagca gagaaacatt caggtctgaa
     4681 ttagacccta cgattctcac acgtttgcca ggcacaaaac cgtcttcttt cttttgaacg
     4741 accacgattt ctttaccatc atctttgcga ataaccattt ccaatgaaga aacttgactc
     4801 gctttttgtt ctacttgatt accaattacc gcaccggcaa ctgcccccac tgcggttgca
     4861 atcgcttgcc ctgtaccacc gccaatcgca ttaccggcaa cgccacctaa tacaccaccg
     4921 ccgacagtac cgatcacacc ttcactgcgt gcttgaattt tcacatcacg cactgaaaca
     4981 atagtgccat aagcgataga acgagcttct tttgcctgct caccggaata aacgcttccg
     5041 ctaaacacgt ctgtatttgc acagccaact aaagcaaaac ttgacattaa tgcgacagct
     5101 aaagctaatt ttttcatttt gtttgttctc cgaaacgtta attttctgac ataattacac
     5161 tttttatatc ataagggtgg agctttagac aataaggatt acaaaaagtt acatttctca
     5221 agctgatcgc aatgcttgga tttggcaatc tctccccttc tacctgaggt tggctgattt
     5281 ttaatttaat ctcagaatta tcagcaagtc taaagtgcga caatgccctt gacacccatt
     5341 gttccgaact ttcgttgtct aacatcggaa aaaccagctc aatatgttcc cagccttctt
     5401 ctaaataaac cttatttttc ggaaaaggca actcgacaat ctttacaaat tgtccgcaaa
     5461 attcaaccgc ttgtattaat gcaaacagcc ctatcggtct gccgtttacc tcgctctctt
     5521 taagtaaggt tgccccatca agcaaaagga tcttccactg ttctgcggtt tcggtttggt
     5581 tcattcttac cgcaagatga tcgatttcat acgctgctaa atcaatgccg gcgatttcag
     5641 caatttgtcg gattttctgt tcaaattgcg ataaatcgcc aaaacaagcg gtcattttct
     5701 cgaaaaattt tgcattttct gctataa
//
LOCUS       DANLYI010000154         5675 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.155, whole genome shotgun sequence.
ACCESSION   DANLYI010000154 DANLYI010000000
VERSION     DANLYI010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 5675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5675
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.155"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            37..1719
                     /gene="recN"
                     /locus_tag="PXD81_001964"
     CDS             37..1719
                     /gene="recN"
                     /locus_tag="PXD81_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HDL5537964.1"
                     /translation="MLTQLTINNFAIVRHLILELNEGMSVITGETGAGKSIAIDALGL
                     CLGYRSESGMIRNGSDKADISATFTMQPHSPAYLWLQEHELLDEDNPQECILRRMINI
                     DGRSKAFVNNRSLPVSQLRELGQYLIHLNGQHAPQLLLKNEYQLEIVDHYATLQPLLM
                     KMAKQYGNWKELNKQVKNFQKQCQENEARKQLLEYQVEELDEFAIKANEFEEMEEKYN
                     ILSSSEELTELSDEITRLLSDDEINANSLIYKALRNAENLAEVDSQYQGILDMLNEAL
                     IQIQEASSEVAQLASKIEQDPELLQELDNRIAQTISLARKHHIQPNELWQYHATLKAE
                     LQELLDFEDNEEQLIQNEKQAYQECITLAEEIYQKRLDATHKLSEQVTKQIKHLAMEN
                     GEFFIHIEHNLEKMSANGADYVEFNLRSNLGQQPQPLAKIASGGELSRISLAVQVLTA
                     NKLSTPTIIFDEVDVGISGSAATTVGKLLRKLGEKCQVLCVTHLPQVASYGNQQFNVQ
                     KYVENNQTETQMTLLTEAERIHALARLLGGSEITETVLANAKEMLNLAKNNE"
     gene            complement(1766..1841)
                     /locus_tag="PXD81_001965"
     tRNA            complement(1766..1841)
                     /locus_tag="PXD81_001965"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1806..1808),aa:Ala,seq:ggc)
     gene            complement(1921..3360)
                     /gene="gltX"
                     /locus_tag="PXD81_001966"
     CDS             complement(1921..3360)
                     /gene="gltX"
                     /locus_tag="PXD81_001966"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007526628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HDL5537965.1"
                     /translation="MKIETLFPLDPNVKVRTRFAPSPTGYLHVGGARTALYSWLYAKH
                     NDGEFVLRIEDTDLERSTPEATAAILEGMEWLDLAWEHGPYFQTKRFDRYNQVIDQML
                     EAGTAYRCYCSKERLDEVRAEQEANKEKAKYDQHCLHNPQNDPSQPHVVRFKNPTEGS
                     VVFDDAVRGRIEIANSELDDLIIRRTDGSPTYNFCVVVDDWDMGITHVVRGEDHINNT
                     PRQINILKALGAPIPTYAHVSMINGDDGQKLSKRHGAVSVMQYRDDGYLPEALINYLV
                     RLGWGHGDQEIFSRDEMIELFDIHSVSKSASAFNTEKLQWLNQHYIRSLAPSYVAKHL
                     EWHMQTQGIDFTSGPKLEEIIPVLGERCKTLKELAESSRYFYQEFKAYDEKAAAKNFK
                     AEAVAPLAKLLEKLTACNDWTVENIHEAMNQTAAELEIGMGKVGMPFRLAVTGSGQSP
                     SMDITAKLVGKERTLARLQKAIEFIQANS"
     gene            3535..3610
                     /locus_tag="PXD81_001967"
     tRNA            3535..3610
                     /locus_tag="PXD81_001967"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3568..3570,aa:Val,seq:tac)
     gene            3659..3734
                     /locus_tag="PXD81_001968"
     tRNA            3659..3734
                     /locus_tag="PXD81_001968"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3692..3694,aa:Val,seq:tac)
     gene            3787..3862
                     /locus_tag="PXD81_001969"
     tRNA            3787..3862
                     /locus_tag="PXD81_001969"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3820..3822,aa:Val,seq:tac)
     gene            3911..3986
                     /locus_tag="PXD81_001970"
     tRNA            3911..3986
                     /locus_tag="PXD81_001970"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3944..3946,aa:Val,seq:tac)
     gene            complement(4140..5633)
                     /gene="mltF"
                     /locus_tag="PXD81_001971"
     CDS             complement(4140..5633)
                     /gene="mltF"
                     /locus_tag="PXD81_001971"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HDL5537966.1"
                     /translation="MKGLIARLFIAVALLLWAWDMIFPWQKIIHSEANHYTQIQQSKT
                     LKVGMINHPLSYFVGPEGKSGIEYDLATAFAQYLKVNLDIKTYDSSEKLFEALRNNDV
                     DIAAAGLLYHSELNDEFQIGPSYYSASWQLVYKKGSARPYRLSDLRENVIIPAGSAVI
                     PILNQLKVDNPELKWQISDKFTQEELLLKVAQGEISHTIAMSVDISAAQHINPNLAVG
                     FDLTDENPVLWYLANSSYSELQSVLLDFMSYSMETGLISRIEEKYFNHLAKFDYVDIQ
                     SYLNAIKTILPKYEPLFRAHKGELEWQMLAAIAYQESHWDPNATSPTGVRGIMMLTKD
                     TADRMKISDRTNPAQSIKAGSEYLHMLMRQMPETIPQADRIWYSLAAYNMGLGHLLDL
                     RRLTKQLGGDPDNWLDVKKNLPLLAEKRYYANLKYGYARGYEALQYVENIRRYYGSII
                     NYQRVEEQKMQEQENLPDGQIKQENSSYEKIEEKEIPTDKEITPASN"
BASE COUNT         1715 a         1176 c         1112 g         1672 t
ORIGIN      
        1 ctatataccc atacagttaa tcttgtgtgg tgtattatgc ttactcaact tactattaat
       61 aattttgcga ttgttcgcca tcttattctt gaattaaatg aaggtatgtc tgttattacc
      121 ggtgaaaccg gtgccgggaa atcaatcgct attgatgcct tagggctatg cttgggttat
      181 cgttctgaaa gtgggatgat tagaaacggt tcggacaaag cagatataag tgcaaccttt
      241 actatgcagc cacatagccc tgcttattta tggctacaag agcatgaact gcttgatgaa
      301 gataatcctc aagagtgtat cttacgccgt atgattaata ttgatggacg ctcaaaagcc
      361 tttgtaaata accgatcatt acctgtttca caacttcgag agttggggca atatcttatt
      421 catttaaatg ggcaacacgc accacaatta ctcctaaaaa acgaatatca acttgaaata
      481 gtcgatcatt atgccacgtt acagcctcta ttgatgaaga tggcaaagca atatgggaat
      541 tggaaagaac tgaataagca ggtaaaaaat ttccaaaaac agtgccaaga aaatgaagct
      601 cgcaaacagc tattagaata tcaagttgag gaattagatg aatttgcaat taaagcaaac
      661 gaatttgaag aaatggaaga gaaatacaat atcttgtctt cttccgagga gcttactgag
      721 ctttcagatg aaatcacacg cttattaagt gatgatgaaa tcaatgcaaa cagtctgatt
      781 tataaagcgt tacgcaatgc agaaaacttg gctgaggttg atagccaata tcagggcatt
      841 ttagatatgc ttaatgaggc cctgattcag attcaagaag caagctccga agtggctcaa
      901 ttagccagta aaattgaaca agatccggaa ttactacagg agctggataa tagaattgca
      961 caaactatat cgttagcacg caaacaccat attcaaccga atgaattatg gcaatatcac
     1021 gcgacattaa aagcagaact acaggaatta ctggattttg aagataatga agaacaactc
     1081 attcaaaatg aaaaacaagc ttaccaagag tgtataacat tagcagaaga aatttaccaa
     1141 aaacgtctag atgccacaca taagctctct gaacaagtaa caaaacaaat taaacactta
     1201 gcaatggaaa acggtgagtt ctttattcat atagaacata atcttgaaaa aatgtctgca
     1261 aatggtgctg actatgtcga gtttaattta cgcagcaatt taggacaaca accccaacca
     1321 ctggcaaaaa ttgcttctgg tggtgagcta tctcgcattt ctctggcagt acaagtgctt
     1381 accgcaaata aactttctac gccaactatt atttttgatg aagttgatgt tgggattagt
     1441 ggctctgctg ccactacagt cggaaagcta ctgcgtaagc taggcgaaaa atgccaagta
     1501 ttatgtgtaa cccatttacc tcaagtggca agttacggca accagcagtt taatgtacaa
     1561 aaatatgtag aaaataatca aactgaaacg caaatgacat tactcacaga agcagagcgt
     1621 attcacgcct tggctcgact attaggcgga agcgaaatca ccgaaacggt tttagctaat
     1681 gcaaaagaaa tgctaaattt agcgaaaaat aacgaatagc caaaaagaaa aatcgcatca
     1741 atgaatattg atgcgattat gaatttggtg gaggtaaacg ggatcgaacc gatgacctct
     1801 tgcatgccat gcaagcgctc tcccaactga gctatacccc cttaatacaa gcggaataat
     1861 atgctttccg cttgaagatg tcaatggctt taatcaagca aggtgttgat tgattaaaaa
     1921 ctagctattc gcctgaataa actcaatcgc tttttgtaag cgagctaagg ttctttcttt
     1981 accgactaat ttggcggtga tatccattga cggcgattgc ccacttcctg ttaccgctaa
     2041 acggaacggc ataccgactt tgcccatacc aatttccagc tcggcagcgg tttggttcat
     2101 tgcctcgtgg atattttcca ccgtccagtc attgcaagcg gtcaattttt ccaataattt
     2161 tgcaagtgga gcaacggctt cggctttgaa gtttttagca gcagcttttt cgtcgtaagc
     2221 cttaaattct tgatagaaat agcggctgct ttcggcaagc tcttttaagg ttttgcaacg
     2281 ttcgcccagt acagggataa tttcttccaa ttttggaccg cttgtgaaat caatgccttg
     2341 agtttgcata tgccattcaa gatgttttgc cacataactt ggggcaagac tgcggatata
     2401 gtgttggttc aaccattgta atttttcagt gttaaatgca cttgccgatt tactgactga
     2461 atgaatgtcg aacagctcga tcatttcgtc acgagagaaa atctcttggt cgccgtgtcc
     2521 ccagcctaaa cgcacaaggt agttaattaa ggcttccggt agatagccgt catcacgata
     2581 ttgcattacg ctcaccgcac cgtggcgttt ggagagtttt tgcccgtcat cgccgttgat
     2641 cattgaaacg tgggcgtagg ttgggattgg tgctcctaat gctttcaaaa tgttgatttg
     2701 gcgtggtgta ttgttgatat ggtcttcacc ccgcacaacg tgagtaatgc ccatatccca
     2761 gtcgtccacc accacacaga aattgtaggt cggcgagccg tcagtacggc gaataatcaa
     2821 gtcatctaat tcgctattgg cgatttcaat tcgcccacgc accgcatcgt caaatactac
     2881 cgagccttcg gtcgggtttt tgaaacgcac aacatgtggt tggctcgggt cgttttgcgg
     2941 attgtgtaag cagtgctgat cgtatttcgc tttctcttta tttgcttctt gctctgcacg
     3001 tacttcgtct aaacgttctt tcgagcagta gcaacggtat gccgtgccgg cttctaacat
     3061 ttggtcgatc acttggttat agcgatcgaa acgcttggtt tgaaagtatg ggccgtgttc
     3121 ccacgccaaa tccagccatt ccataccctc taaaatagcg gcggttgcct caggggttga
     3181 gcgttcaaga tcggtatctt caatacgtaa cacaaattca ccatcattat gcttagcata
     3241 taaccaagag tagagcgcgg tcctcgcccc acctacgtgc aagtagccag ttgggctagg
     3301 tgcaaaacgg gtacggactt ttacattcgg atctaagggg aaaagggttt caattttcat
     3361 tttatattgc ctaattgatg attcttaaga aaaacttgtc tattttacta cgcttgaggg
     3421 tgaatcatag taaaaaatta aatttgcggg gtaaaaaatc agcaaacggg gtaaaaagaa
     3481 caaaaagggt ttgacgaaac agcaaaaatc tctataattc acttccacaa cagcgggcga
     3541 ttagctcagt tgggagagca cctcccttac aaggaggggg tcactggttc gagaccggta
     3601 tcgcccacca cttcaactta agtgtttcgt tgttgtatca caatctaatt ttgaaaacgg
     3661 gcgattagct cagttgggag agcacctccc ttacaaggag ggggtcactg gttcgagacc
     3721 ggtatcgccc accacttcaa cttaagtgtt tcgttttcaa attccctttg attttaagtc
     3781 tcaagtgggc gattagctca gttgggagag cacctccctt acaaggaggg ggtcactggt
     3841 tcgagaccgg tatcgcccac cacttaagac taaaatcata aacttcaaat ggcgattttt
     3901 cgtatctgaa gggcgattag ctcagttggg agagcacctc ccttacaagg agggggtcac
     3961 tggttcgaga ccggtatcgc ccaccatcta tttagtatca aagcctaaaa cttatatcat
     4021 aatctaataa cccctttaaa gtcataacta aaaagggaaa ataaattcag atggcctgtt
     4081 gcatctgcat aaatgccttt ctacgtgcag aaattaatct ttttctatct ttaatttgct
     4141 taatttgacg caggcgtaat ttccttgtca gtagggattt ctttttcttc aattttctca
     4201 taagaagaat tctcctgttt tatttgacca tctggtaaat tttcttgttc ttgcatcttt
     4261 tgctcttcaa ctcgttgata attaataata ctaccataat aacgccgtat attttcaaca
     4321 tactgtaatg cttcatatcc tcgagcatag ccgtatttta gattagcata ataacgtttt
     4381 tccgcaagca atggcagatt ctttttaaca tcaagccaat tatccggatc accaccaagt
     4441 tgttttgtta atcgacgaag atccaacaag tgccccaatc ccatattata cgctgctagg
     4501 ctataccaaa ttctatctgc ttgaggaata gtttccggca tttgtctcat tagcatatgt
     4561 aaatattcag atcccgcttt gatactttgt gcagggttag tcctatcaga aattttcatt
     4621 ctatctgccg tatcttttgt cagcatcata attcccctca ctccggttgg agaagttgca
     4681 ttaggatccc aatgagactc ctgataggcg atagccgcca gcatttgcca ttctaattca
     4741 cctttatgtg cccgaaaaag gggttcatat ttaggtaaaa tagttttaat tgcattcaaa
     4801 tagctctgaa tatcaacata atcaaactta gctaaatgat taaaatattt ctcttctatt
     4861 cttgatatca gcccagtttc catagaataa gacataaaat ccagcagcac agattgcagt
     4921 tcactataag aactatttgc taaataccat aacactgggt tttcatcagt taaatcaaac
     4981 cctacggcta aattaggatt aatatgctgt gcagcagaaa tatcaaccga cattgcaatt
     5041 gtatgtgaaa tttcaccttg tgcgactttt aacaatagct cttcttgtgt aaatttatcc
     5101 gagatctgcc attttagttc cggattatcc accttaagct gatttaatat tggaattacc
     5161 gcagaaccgg ctggaataat cacattttcc cgcaaatcgg aaagtcgata tggtctagca
     5221 cttccctttt tataaacaag ctgccaagaa gccgaataat aggaaggacc tatctgaaat
     5281 tcatcgttta attcagaatg gtaaagcagc ccagcggcag caatatcaac atcattattt
     5341 cttaaggcct caaacaattt ctcgctacta tcataagttt taatatcaag attgaccttt
     5401 aaatattgag caaaggctgt tgctaaatca tattcaatgc cactttttcc ttcaggacca
     5461 acaaagtaag aaagtggatg gttgatcatt ccaaccttta acgttttgct ttgctgaatt
     5521 tgtgtataat gatttgcttc tgaatgaatg attttttgcc aaggaaaaat catatcccaa
     5581 gcccacagta acaatgctac ggcaataaaa aggcgagcaa ttaatccttt caatttaaaa
     5641 tcctcattat ttcaagctaa gcattaagcc tatct
//
LOCUS       DANLYI010000155         5636 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.156, whole genome shotgun sequence.
ACCESSION   DANLYI010000155 DANLYI010000000
VERSION     DANLYI010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 5636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5636
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.156"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            complement(54..130)
                     /locus_tag="PXD81_001972"
     tRNA            complement(54..130)
                     /locus_tag="PXD81_001972"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(94..96),aa:Val,seq:gac)
     gene            complement(194..994)
                     /gene="apaH"
                     /locus_tag="PXD81_001973"
     CDS             complement(194..994)
                     /gene="apaH"
                     /locus_tag="PXD81_001973"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     ApaH"
                     /protein_id="HDL5537967.1"
                     /translation="MATYIVGDLHGCFNEFQLLLEKVNYNPKQDELFLTGDLVARGED
                     SLACLRFVKDSANNAKTVLGNHDLHLLGIKRVKPNDKIDAIFEAKDRLDLQNWLRNQP
                     LVIHHPKHQFLLTHAGISPEWDLATTLACAKEAEKVLQSDNYADYIAQMYENKPERWS
                     EDLQGVERWRYIINVFTRMRFCYTDKRLDFDCKLPLEQAPQELLAWFDLDNPLFTQKN
                     IIFGHWASLMGKCTRPNIYALDTGCAWGSHLTMLRWEDKQIFTQKRLK"
     gene            complement(1086..3062)
                     /locus_tag="PXD81_001974"
     CDS             complement(1086..3062)
                     /locus_tag="PXD81_001974"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005617246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HDL5537968.1"
                     /translation="MFQNNPLLAQLKQQIEASKEYVAGVVKASDKAFGFLECDKKSYF
                     IPPAEMKKVMHGDSVKAVVKRDGDKEQVEIDSLLEPMLDRFIAQVRLNKEGKLQLAVD
                     HPSIKQTIPANTHKKATEKLENGDWVVAQLKTHPLRDDRFFFAQVTQFICKESDNFAP
                     WWVTLARHEQPREPVANEKNYELHDELAREDLSHLYFTTIDSPSTQDMDDALFIEPIK
                     QNGKRTGWRLVVAIADPTAYIPENSHIEKAARQRCFTNYLPGFNIPMLPRELSDDLCS
                     LVPNEKRPALVGFIETDLNGNLIKETTFTSAWVESKDRLAYDDVSDYLEGVENAWQPQ
                     SEETKQQIEWLHQFTQARIQWRAENALLFKEQGDYTFELNEDGSVKDIHVEYRRIANQ
                     MIEESMILANICCAKFLSQHAKTGVFNTHSGFDPKNYELVKSFLLQTLASDENRDELA
                     ERYSAERLATLEGYCQMQRDIQDYPEKFLELRLRRYLTFAEFKAEVAPHFGLGITDYA
                     TWTSPIRKYGDMVNHRLIKQVLLGKQSQAVEESVLSRLQEARRQNRLVERDIADWLYA
                     RYLAPMVEQAVEFECEIADVSRGGLRAKVIKNGAQIFVPFSSLHDNKEEMEYRQEELA
                     LYIKGEKAYQIGQAVKVKLTEVRLETRSIVGNII"
     gene            3222..3500
                     /locus_tag="PXD81_001975"
     CDS             3222..3500
                     /locus_tag="PXD81_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bor family protein"
                     /protein_id="HDL5537969.1"
                     /translation="MKKLLAIATVFSLAACSTQTLELSPAKEAQYDRVQHFFVSGLAQ
                     KQEIDAAKICNGADKVGKVETETTFFNGLLGTITYGIYSPRQIRVYCN"
     gene            3525..3821
                     /locus_tag="PXD81_001976"
     CDS             3525..3821
                     /locus_tag="PXD81_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bor family protein"
                     /protein_id="HDL5537970.1"
                     /translation="MKKLLAMSVLTALLTTACTTQTAHIGGKVTDNIKPTKSVTQSFF
                     IGGIGQEETLNVVEVCGSKDKVQQVETVLSGGDILLGLVTIGIYTPRTANVYCK"
     gene            complement(3874..4665)
                     /locus_tag="PXD81_001977"
     CDS             complement(3874..4665)
                     /locus_tag="PXD81_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDL5537971.1"
                     /translation="MGILTGKRILVTGLASNRSIAYGIANAMKQQGAELAFTYLNDKL
                     KPRVEEFAKEFGSDIVLPLDVATDESITECFTELSKHWEKFDGFVHAIAFAPGDQLDG
                     DYVNAATREGYRIAHDISAYSFVAMAQAARPFLNENAALLTLSYLGAERAIPNYNVMC
                     LAKASLEAATRVMAADLGKEGIRVNAISAGPIRTLAASGIKNFKKMLSAFEKTAALRR
                     TVTIDDVGNSAAFLCSDLASGVTGEVLHVDAGFSVMAMGELGDEE"
     gene            complement(4809..5582)
                     /locus_tag="PXD81_001978"
     CDS             complement(4809..5582)
                     /locus_tag="PXD81_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FNR family transcription factor"
                     /protein_id="HDL5537972.1"
                     /translation="MKIVSEPKTSGRHTCTIHCQNCSISQLCLPFTLNETELTQLDNI
                     IERKKPIQKSQVIFKLGDELRSLYAIRSGTLKAYTLSESGEEQITGFHLPGDLIGFDA
                     ITNMQHEGYAQALETSMICEIPFDILDDLAGKMPKIRHQIMRLMSNEIKSDQEMILLL
                     SKMNAEEKLAAFLYNLSQRYSARGFSAKEFRLTMTRGDIGNYLGLTIETISRLLGRFQ
                     KSGMISVQGKYIIINRMEELAELAGAIKPQQNIIAQTTA"
BASE COUNT         1617 a         1095 c         1170 g         1754 t
ORIGIN      
        1 tataagtatg gtaagattaa agcctgaatc gggatattca ggcttaaaaa taatggtgcg
       61 actagctgga ctcgaaccag tgacccccac catgtcaagg tggtgctcta accaactgag
      121 ctatagtcgc attaacggtg tgcattctat tggaaaaagg aacttagtgc aatatgcttt
      181 tggtcgaatg cttttatttt agacgctttt gggtaaaaat ttgtttatct tcccaacgga
      241 gcatggttaa atggcttccc caagcacagc cggtatcaag tgcatagatg tttggacgag
      301 tgcatttgcc cattaggctt gcccagtgac caaagataat gtttttctgt gtaaataacg
      361 gattatctaa gtcaaaccaa gccagcagtt cttgaggggc ttgttcaagc ggtaatttac
      421 agtcaaaatc taaccgcttg tcggtgtagc aaaaccgcat acgggtaaac acattgatga
      481 tataacgcca acgttcaaca ccttgcaaat cttcactcca gcgttcgggc ttattctcat
      541 acatttgggc gatgtagtcc gcataattgt cactttgtag caccttttct gcctctttgg
      601 cacaagctag ggtagttgct aaatcccact cagggctgat tcctgcgtga gtcagcaaaa
      661 attgatgttt ggggtgatga attacaagcg gttgatttct gagccaattt tgcaaatcga
      721 gccgatcttt agcctcaaaa atagcatcaa tcttatcatt tggttttacc cgtttgatgc
      781 caagcaagtg caaatcgtgg ttgcctagca cggttttggc attgtttgca gagtctttca
      841 caaaacgtaa acaggctaaa gagtcctcac cacgggcgac taaatcccct gttaaaaaga
      901 gttcgtcttg cttggggtta taattgactt tttctaataa aagctgaaat tcgttaaagc
      961 aaccgtgtag gtcgcctaca atataagttg ccattgtttc tcctttgttc ccctaaagtt
     1021 ataaacaagc ggttatattt tagagaaaat ttgcaaaatc tcatcaaaat ataaccgctt
     1081 gaaatttaaa tgatatttcc tacgattgag cgggtttcta agcgtacttc ggtgagtttc
     1141 actttcaccg cttgcccgat ctgataggct ttttcacctt taatataaag tgcaagttct
     1201 tcttggcggt attccatttc ctctttgttg tcgtgcaagc tggagaatgg cacaaaaatt
     1261 tgagcaccgt tttttatgac tttcgcacgc aagccaccac gggaaacatc tgcaatttcg
     1321 cactcaaatt cgaccgcttg ttccaccatt ggggcaaggt aacgagcata aagccagtca
     1381 gcaatatcac gttctactaa acggttttga cggcgagctt cttgtaaacg gcttaaaact
     1441 gattcttcca ccgcttggct ttgtttgcca agtaacactt gcttgattaa acggtggttt
     1501 accatatcgc catatttacg gatgggtgat gtccaagtgg cgtaatcggt aatgcctagt
     1561 ccgaaatgcg gggcaacttc ggctttaaat tcagcgaagg ttaaataacg tcgtaaacgc
     1621 aactctaaga atttctctgg gtagtcttga atatcacgtt gcatttggca atagccttct
     1681 aaggttgcta aacgctcggc ggagtagcgt tcggcaagct catcacgatt ttcatcgctg
     1741 gctaaagttt ggagtaaaaa tgattttacc aattcataat ttttcggatc aaaacctgaa
     1801 tgggtattaa atacaccggt tttggcatgt tggcttaaga attttgcaca acaaatattg
     1861 gcaagaatca ttgattcttc gatcatttga ttcgcaatac ggcgatattc aacgtgaata
     1921 tcttttaccg agccatcttc atttaactca aaggtataat cgccttgttc tttgaatagc
     1981 aacgcattct cggcacgcca ttgaatgcgg gcttgagtaa attgatgtag ccactcaatt
     2041 tgttgttttg tttcttcaga ctgaggttgc cacgcatttt ccacgccttc gagataatcg
     2101 gaaacatcgt cgtaagccaa gcgatctttt gattcgaccc aagcagaggt aaaggttgtc
     2161 tctttaatga gattgccgtt taaatcggtt tcaataaagc caacgagggc agggcgtttt
     2221 tcattcggga caagcgaaca gagatcgtct gagagttctc gaggtaacat tggaatgtta
     2281 aagcccggca aatagttggt gaaacaacgc tggcgagcgg ctttttcaat atgggaattt
     2341 tctgggatat aagcggttgg gtcagcaatc gctaccacta aacgccagcc ggttcgcttg
     2401 ccattttgct taattggttc gatgaacaga gcatcgtcca tatcttgcgt gcttgggctg
     2461 tcaatagtgg taaagtaaag atgagataaa tcttcacgag ctaattcatc gtgtaattca
     2521 tagttttttt catttgcgac cggttcacga ggctgttcgt ggcgggcaag tgtgacccac
     2581 caaggggcaa aattatcaga ttctttacag ataaattggg taacttgagc aaagaaaaaa
     2641 cggtcgtcac gcaatgggtg cgtttttaat tgtgcaacca cccagtcgcc attttctaat
     2701 ttttcagtgg cttttttatg ggtatttgcc gggatagttt gcttaatgct tgggtgatca
     2761 accgcaagtt gcaatttgcc ctctttattc aagcgaactt gggcaataaa tctatccagc
     2821 attggctcaa gtagtgaatc aatttctacc tgttctttgt cgccatcacg tttgactacg
     2881 gctttcactg aatcaccgtg cattactttt ttcatttccg caggtggaat aaaatagctt
     2941 tttttatcgc attctaaaaa accgaatgct ttgtctgagg ctttgacaac gcctgcgaca
     3001 tattctttgc ttgcttcaat ttgttgtttg agttgggcta aaaggggatt gttttgaaac
     3061 ataatttctc attttaaaaa taaaaaaagg tgctatcgtt gcaccgaaaa aaacaatggc
     3121 attataacgg atttgaacca agttcagcaa ataaatgctg ttgctataac ttataaaggt
     3181 tggtagaata taatccattt aaaaataatg gagacttttc aatgaaaaaa ttacttgcaa
     3241 ttgcaacagt attttcttta gcagcctgtt ctactcaaac acttgaacta tctcctgcta
     3301 aggaagctca atatgatcgt gttcaacact tctttgtttc agggcttgct caaaaacaag
     3361 agatagatgc agcaaaaatt tgtaatggtg cagataaggt aggtaaggta gaaactgaaa
     3421 ctactttctt taatggcttg ttaggtacaa ttacttatgg tatctattca cctcgccaaa
     3481 tccgtgtata ttgtaactaa tcctagtaat ttaaggagtt gaaaatgaaa aaattgcttg
     3541 ctatgtctgt attaacagcc ctgctgacta cagcatgtac aacacaaact gcccatattg
     3601 gcggcaaagt tactgataat attaaaccaa ctaagagtgt aactcagtcg ttctttatag
     3661 gtgggattgg gcaagaagaa acattgaatg ttgtagaggt ttgtggttcc aaagataaag
     3721 tgcagcaggt tgaaacagta ctttcaggtg gggacattct attaggtctt gtgactatcg
     3781 gtatttatac acctcgtact gcaaatgtat attgtaagta atattttatt aaaaaaataa
     3841 cggactcaat tgagtccgtt atttttatag gaattactct tcgtcgccta attcacccat
     3901 tgccattacg ctgaagccgg catcaacgtg taacacttca cctgttacac cagaggctaa
     3961 atctgagcat aagaaagctg cagagttacc tacatcatca atagttacgg tgcggcgtaa
     4021 agcagcggtt ttctcgaaag cagaaagcat tttcttgaag tttttaatgc ctgaagcagc
     4081 taatgtacgg atcgggccgg ctgaaatcgc atttacacga ataccttctt tacctaaatc
     4141 ggctgccatt acacgggttg cagcttctaa agaggctttg gctaagcaca ttacattgta
     4201 gttggggatt gcacgctctg cacctaagta acttaaggtt aaaagggcgg cattttcatt
     4261 taagaagggg cgagcagctt gagccattgc aacaaagcta taggcactga tgtcgtgtgc
     4321 aatacgataa ccttcacggg ttgcggcatt tacataatcg ccgtctaatt gatcgcccgg
     4381 tgcaaatgcg atagcgtgaa caaaaccgtc aaatttttcc caatgtttgc ttaattcagt
     4441 aaagcactca gtaatgcttt catcggttgc tacatctaac ggaaggacaa tgtctgagcc
     4501 aaattctttg gcaaattctt caactcgtgg ttttaattta tcgtttaagt aggtgaatgc
     4561 aagttctgca ccttgttgct tcattgcgtt tgcaataccg taagcgatcg aacggttgct
     4621 tgctaaacct gtcactaaaa tacgtttacc agttaagata cccataaaaa ttcctctttt
     4681 atattgattg gggcaaaatt gtgtcgatta taacggatat aggaaaaacg ggcaaatgga
     4741 gttagcggct catcggatca gaaatccctt tttgaacgag gtttcaaaaa gggatttggt
     4801 aagataaatt aagcggtagt ttgagcaata atattttgct gcggtttgat tgccccggct
     4861 aattcggcta attcttccat acggttaata ataatatatt ttccttgtac tgaaatcata
     4921 ccgctttttt ggaaacgccc taataaacgg ctgatagttt caatggttaa accaagataa
     4981 ttgccgatat ctccacgggt catagttaga cggaactctt ttgcagagaa accacgggct
     5041 gagtaacgtt gggaaagatt atacaaaaat gcagctagtt tttcttccgc attcattttt
     5101 gagagcaata gaatcatttc ttgatcgctt ttaatttcat tactcatcaa acgcataatt
     5161 tgatggcgaa tttttggcat tttgccggca agatcatcta aaatatcgaa tggaatctca
     5221 caaatcattg aggtttcgag tgcttgagca tagccttcgt gctgcatatt ggtaattgca
     5281 tcaaagccaa ttagatcgcc aggtaaatgg aaaccggtaa tttgctcttc accactttca
     5341 cttaaggtat aggcttttaa tgttccagaa cggatagcat aaagcgaacg cagttcatca
     5401 cctaacttaa aaataacttg cgatttttga atcggttttt tacgctcaat aatattatct
     5461 agttgagtta attccgtttc attaagagta aaaggtaagc atagttggct aatactgcaa
     5521 ttttgacaat gaattgtgca agtatgacgt ccgcttgttt taggttctga tacaattttc
     5581 atccattcaa ccttaaatta gaaaagtatt cctaaatttg atttagaaca aagttt
//
LOCUS       DANLYI010000156         5620 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.157, whole genome shotgun sequence.
ACCESSION   DANLYI010000156 DANLYI010000000
VERSION     DANLYI010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 5620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5620
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.157"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            <1..121
                     /locus_tag="PXD81_001979"
     CDS             <1..121
                     /locus_tag="PXD81_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nitrate/trimethylamine N-oxide reductase
                     NapE/TorE"
                     /protein_id="HDL5537973.1"
                     /translation="LKTLLLSGLILPLLMAFAVGAYGFIVWMLQLFVIGLPGA"
     gene            125..1288
                     /gene="torC"
                     /locus_tag="PXD81_001980"
     CDS             125..1288
                     /gene="torC"
                     /locus_tag="PXD81_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentaheme c-type cytochrome TorC"
                     /protein_id="HDL5537974.1"
                     /translation="MLGSIKALFTRPATKIGLGVLVGIGFIAGAISWHQFNKAMDHTS
                     TEEFCVSCHSMQQPLEELKKTAHWSNKSGVTATCSSCHLPHDYTEKYARKVQASREAW
                     AELSGKYKEEGSFEKHRLEMAEREWARFAANGSKECKACHSYERMNFDKMGEAARKAM
                     KPAAEKDQSCMDCHKGIAHHLPKADENALKSKFDQFVNKSPNQGKSYYTKGNAELFLD
                     EGLSQAIGQLETAVPVAFVQAGKNADLVELTMWRKEKGFGRIWYHDFGKNITDAVLTK
                     EFMANQPKFEVLETKEDPITGLNWQKVKLQAWIGKAQLLDDVTPIWANAENQYKTQCS
                     TCHRQPDVAHFDSNSWIGLFNGMVGFTNMDKQTGKEVLRYLQMHASDSEEAKH"
     gene            1388..3853
                     /gene="torA"
                     /locus_tag="PXD81_001981"
     CDS             1388..3853
                     /gene="torA"
                     /locus_tag="PXD81_001981"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
                     /protein_id="HDL5537975.1"
                     /translation="MQQSRRQFLKNMSVMAAAVTMPNFLVPRNVFASEDLSQWKVSGS
                     HWGAMRAKIENGRVAEIKPFEFDKHPTEMLNGIKGLIYSDSRIRYPMVRLDWLKNRHN
                     SNTAQRGDNRFVRVTWDEALDLFYQELERVQKEYGPWALHTANVGWRSTGQFHSCGNH
                     MIRAIAMHGNSVGTAGDYSTGAGQTILPYVLGSTEVYSQGTSWEIILKESENIIFWAS
                     DPVKNLQVGWNCETHEAYAYLEKLKEKVAAKGVNVICVDPVKSKTQNFLGCEQQYINP
                     QTDVPFMLALAHTLYTENLYDKKFLDMYTVGFEKFLPYLLGETEDKVAKTPEWAAEIC
                     GISADKIREFARMLAGKRTQFIFGWAIQRQQHGEQPYWMGAVLASMLGQIGLEGGGIS
                     YAHHYSSIGIPESGAAMPGAFPLNIDEGQTPKYDNKDYKGYSDTIPVARMTDSLLHAG
                     ETIDYNGKKVTYAPFKMAIFTGCNQWHRHSERNKMKQAFQKLETIVSINYSWTATCRF
                     SDIVLPACTPFERNDIDAYGSYSNRGVIAMHKLVDPLYDSRSDFEIFRDLCRRFGKEK
                     EYSRGMDEMQWVEKLYKDCRRENRGKFEMPEFAEFWQKGYVLFPEGKPWVRHADFRED
                     PELHALGTPSGFIEIYSNKIASFGYDDCKGHPMWFEKAERSHGGKNSDKFPYWLQSVH
                     PDKRLHSQLCESKELRETYSVQGREPLYINPQDAAKHGIQDGDLVRVFNDRGQAIVGA
                     VLSDNFPTGVVRLQEGAWYSPLDESVGAIDTYGDPNTMTLDIGTSKLAQAVSANTCLV
                     NIEKFIGNAPAPNGFNGAIEVTA"
     gene            3853..4443
                     /gene="torD"
                     /locus_tag="PXD81_001982"
     CDS             3853..4443
                     /gene="torD"
                     /locus_tag="PXD81_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HDL5537976.1"
                     /translation="MTLAISREERQFIYGWFNAMLARELSDEQLNALQAGEFDDFFAY
                     LSELGFAEQVAKFQAEVTACRQLEFPRLELAADFAQLFLLEGNLSAIPYASAYLSGEA
                     LAKNLAEMDRLLGEFGLQVNRETKEPSDHLCVYLTLFDRLLAEKPLETQQQFLQNQLM
                     SWLPAWFAKAKTVKTNTGVYPALLRLFEQVLLKEQR"
     gene            4583..5272
                     /locus_tag="PXD81_001983"
     CDS             4583..5272
                     /locus_tag="PXD81_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HDL5537977.1"
                     /translation="MQNNKTTYLNWYDLCVLTLILFGEAIYTSTQQYFITTEMQGNFE
                     ENLTFSNEQNYWALLTQLALLLIAMGYLKWRNFDFSRWVIRPSWLAFFSGIGLFILLS
                     LAMDLYFETLYGVYNLVYPEPFWAVLREVNISTVLYAILNGFYEEIFFLGICLAVKPE
                     YLKWAVIYSLIIRVSFHTYQGMESALGLGIWLGLIIYLLYSRSKQKNLFPFWVAHSIA
                     DILGLSLLAYF"
     gene            5430..>5620
                     /locus_tag="PXD81_001984"
     CDS             5430..>5620
                     /locus_tag="PXD81_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015586989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5537978.1"
                     /translation="MKSIKKLTLATLILVGLTACGSGGSGSSSNNAKSKPQVELKKTE
                     EDKKTEEDKKTEEDKKTEE"
BASE COUNT         1635 a         1206 c         1357 g         1422 t
ORIGIN      
        1 attaaaaacg cttttgctat cagggctgat tttaccgcta ttgatggcgt ttgccgtagg
       61 agcttacggc tttattgtgt ggatgttaca actgtttgtt attggtttac cgggagcgta
      121 gaaaatgtta ggttcaatca aagcattatt tactcggcct gcaaccaaaa tcgggttggg
      181 agtgttagtt ggtatcggtt ttattgccgg agcaattagc tggcatcaat ttaataaagc
      241 gatggatcat accagcactg aagagttttg cgtgagttgc cactcaatgc agcagccgtt
      301 agaggagctt aaaaaaaccg cccactggtc gaataaaagc ggggtaacgg caacctgttc
      361 aagctgccac ttaccgcacg attataccga aaaatatgcc cgcaaagtgc aggcaagccg
      421 tgaggcgtgg gcagaattat ccggcaaata taaagaagaa ggctcatttg aaaaacaccg
      481 cttagaaatg gcagagcgtg agtgggcaag atttgccgcc aatggttcaa aagagtgtaa
      541 ggcttgccac agctatgagc gaatgaattt tgataaaatg ggcgaagcag ctcgtaaggc
      601 aatgaaaccg gctgcggaga aagatcaaag ttgtatggat tgccacaaag gcattgctca
      661 ccacttaccg aaagccgatg aaaatgcact gaaatcgaag tttgatcagt ttgtgaataa
      721 atccccaaac caaggcaaat cttactacac caaagggaat gccgaattat ttctagacga
      781 aggtttaagc caagctatcg ggcaattaga aaccgccgtg ccggtggcat ttgtgcaagc
      841 gggcaaaaat gcggatttgg ttgagctgac aatgtggcgt aaagagaaag gtttcggtcg
      901 catttggtat cacgatttcg ggaaaaacat taccgatgcg gttctaacca aagagtttat
      961 ggcaaatcag ccgaaatttg aggtgttgga aaccaaagaa gatccgatta ccggcttgaa
     1021 ttggcaaaaa gtgaaactac aagcgtggat tggcaaagct caattattag acgatgttac
     1081 cccaatctgg gcgaatgcag aaaaccaata caaaacccaa tgtagcactt gccacagaca
     1141 gcctgatgtc gcccacttcg attccaacag ctggatcggc ttattcaacg gaatggtcgg
     1201 ctttaccaat atggataaac agaccggcaa agaggtgcta cgttatttac aaatgcacgc
     1261 ctccgatagt gaagaagcga agcattaata atattactcc cctctttact aaagagggga
     1321 attaatcgag cgttgaataa cattctctgt actagctcac gtttttacaa ataaagaagg
     1381 acaagatatg caacaatctc gccgtcaatt tttaaaaaat atgtctgtga tggctgccgc
     1441 tgtcacaatg ccgaatttcc tcgtgcctcg caatgtgttt gcgagtgaag acctcagtca
     1501 atggaaagtt tccggctctc actggggggc aatgcgtgcc aaaattgaga acggacgagt
     1561 ggcagaaatc aaaccgttcg agtttgataa acacccgacc gaaatgctca acggcattaa
     1621 aggcttaatt tacagcgatt cccgtatccg ctacccgatg gtgcgtttag attggctcaa
     1681 aaaccgccat aacagcaaca ccgcacagcg tggcgataac cgctttgtgc gggtaacgtg
     1741 ggacgaagcc ctcgacctgt tctaccaaga gttagaacgg gtacaaaaag agtacggccc
     1801 gtgggcgtta cacaccgcca atgtgggctg gcgttcaacc ggtcaattcc acagttgcgg
     1861 taaccatatg atccgagcga ttgcgatgca cggtaacagt gtcggcacgg caggcgacta
     1921 ttctaccggt gcagggcaga ccattctgcc gtatgtgctg ggttcaaccg aagtctattc
     1981 gcagggcacg tcttgggaaa tcatcttaaa agagagcgaa aacatcattt tctgggcaag
     2041 cgacccggtg aagaacctgc aagtgggttg gaactgcgaa acccacgaag cctatgccta
     2101 cttggaaaaa ctgaaagaga aagtggcagc aaaaggcgtg aatgtgatct gtgttgatcc
     2161 ggtgaaaagc aaaacccaga atttcttagg ttgcgaacag cagtacatca atccacaaac
     2221 cgatgtgccg tttatgttgg cacttgccca cacgctctac accgagaacc tgtacgacaa
     2281 aaaattcttg gatatgtaca cggtcggctt tgaaaaattc ctgccgtatc tgttaggcga
     2341 aaccgaagac aaagtggcga aaaccccgga atgggcggct gaaatttgtg gtatttccgc
     2401 cgacaaaatc cgtgaatttg cccgaatgct tgccggcaaa cgcacccaat ttattttcgg
     2461 ctgggcaatt caacgccaac aacacggcga acaaccgtac tggatgggag cggtgctagc
     2521 ctcaatgtta gggcaaatcg gcttggaagg cggcggcatc agctatgctc accattacag
     2581 ctctatcggt attcctgaat ccggtgcggc aatgccgggg gcgttcccgc tcaatattga
     2641 tgaggggcaa accccgaaat acgacaacaa agattacaaa ggctacagcg acaccattcc
     2701 ggtggctcgt atgaccgaca gcttgctcca cgccggcgaa accattgatt acaacggcaa
     2761 aaaagtgact tacgctccgt tcaaaatggc gattttcacc ggctgtaacc aatggcaccg
     2821 ccattccgag cggaacaaaa tgaaacaggc gttccaaaaa ctggaaacca ttgtttcgat
     2881 caactacagt tggacggcaa cctgccgctt ctccgacatc gtgctgccgg cctgtacccc
     2941 atttgaacgc aacgatattg acgcctacgg ctcatacagc aaccgtggcg tgattgcaat
     3001 gcacaagctg gttgatccac tctacgactc tcgctccgac tttgagattt tccgagatct
     3061 ctgccgccgt ttcggtaaag agaaagagta cagccgtgga atggacgaaa tgcagtgggt
     3121 agaaaaactc tacaaagatt gtcgccgtga aaacagaggt aaatttgaaa tgccggagtt
     3181 tgctgaattt tggcaaaaag gctatgtgct gttcccagag ggcaaacctt gggttcgcca
     3241 cgccgatttc cgtgaagatc cggaattgca cgcacttggc acaccgtccg gctttatcga
     3301 aatttacagc aacaaaatcg cctctttcgg ctatgacgat tgcaaaggtc acccaatgtg
     3361 gtttgagaaa gccgaacgtt cacacggcgg taaaaattcc gacaaattcc cgtactggtt
     3421 gcaatcagtt cacccggaca aacgcttaca ctctcaactt tgtgaatcga aagaactgcg
     3481 tgaaacttac agcgtgcaag ggcgtgaacc gctttacatt aacccacaag atgccgccaa
     3541 acacggcatt caagacggag atttagtgcg agtgtttaac gaccgtgggc aagcgattgt
     3601 gggggcggtg ctttccgaca acttcccgac cggcgtagtg cgtttacaag aaggggcttg
     3661 gtattcaccc cttgacgaaa gcgtgggggc gattgacacc tatggcgacc cgaacacaat
     3721 gaccctcgac atcggcacgt ccaagttggc acaagcggtt tccgccaaca cttgcttggt
     3781 gaacatcgag aaatttatcg gcaatgcccc ggctccgaat ggctttaacg gtgcgattga
     3841 ggtaacggct taatgacgtt ggcaatcagt cgtgaagaac ggcaatttat ctatggctgg
     3901 tttaacgcaa tgctcgcccg agaactttcg gacgagcagt taaatgcctt acaagcgggc
     3961 gaatttgacg atttttttgc atacctcagc gaattgggtt ttgccgaaca ggttgcaaaa
     4021 tttcaagcag aagtgaccgc ttgtaggcag ctggaatttc ctcgtctaga actggcggct
     4081 gactttgccc aactgttttt attggaaggc aatctcagtg ccattcctta cgcctccgcc
     4141 tatttaagcg gcgaagcgtt agcgaaaaat ttggcggaaa tggacagact gctgggcgaa
     4201 tttgggctgc aagttaatcg agaaaccaaa gagccgagcg atcacctttg cgtttatctc
     4261 accctttttg accgcttgct tgctgaaaaa ccgttggaaa cgcaacagca atttctgcaa
     4321 aaccaactga tgagctggct ccctgcgtgg ttcgccaaag ccaaaaccgt caaaactaac
     4381 accggcgttt atccggcgtt gctaaggttg tttgaacagg tgttgctgaa ggaacaaaga
     4441 taaagttatt tatttgagaa tgatttgtat tgagcgaatt gagaaaggaa gttaaaattt
     4501 caacttcctt ttttattggc tggatttatc cacattatta caagcggtta tttttataaa
     4561 aaaatttaca aaggagacat taatgcaaaa taataaaacc acctacctaa attggtacga
     4621 tttatgcgtg ctaaccctga tcctgtttgg tgaggcaatt tatacctcaa cccagcaata
     4681 tttcatcacc accgaaatgc aaggtaattt tgaagaaaat ttaacctttt caaatgaaca
     4741 aaattattgg gcattactga cgcaacttgc attgttgctg attgcaatgg gctatctaaa
     4801 atggcggaat tttgatttca gccgttgggt gattcgtccc tcttggctgg ctttttttag
     4861 cggtatcggg ttgtttatct tgctctcgtt agcgatggat ctctattttg aaacattata
     4921 tggtgtttac aacctagtgt atccggagcc attttgggca gtattgagag aagtgaatat
     4981 ttctaccgtg ctgtatgcga ttttaaacgg cttttatgaa gaaatctttt tcctcggtat
     5041 ttgcttggcg gttaaacctg aatatttaaa atgggcggtg atttattcac tgattattcg
     5101 ggtgagtttt catacctatc aaggaatgga aagtgcgtta ggattaggaa tttggctcgg
     5161 tttaattatt tatttgctct actcacgctc aaagcagaaa aacctatttc cattttgggt
     5221 ggcacacagt attgcggata ttttggggct gagtttgttg gcttattttt agttaaaggt
     5281 gctacattta agcggcataa gttgatagag ctgatcgtac cagtagcata aaaacattat
     5341 tatttgtatg gaaaggtgct tttaggttac aatataaccg atagttattg gttataacta
     5401 aaaattattt ttaatttaac ggagcaaata tgaagagtat caaaaaatta accctagcca
     5461 ctttgatttt agtgggttta actgcttgtg gtagcggtgg ttcaggtagc agttctaata
     5521 atgctaaatc gaaaccacaa gttgagttaa aaaaaactga agaggataaa aaaactgaag
     5581 aggataaaaa aactgaagag gataaaaaaa ctgaagagga
//
LOCUS       DANLYI010000157         5583 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.158, whole genome shotgun sequence.
ACCESSION   DANLYI010000157 DANLYI010000000
VERSION     DANLYI010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 5583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5583
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.158"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            complement(12..548)
                     /gene="hpt"
                     /locus_tag="PXD81_001985"
     CDS             complement(12..548)
                     /gene="hpt"
                     /locus_tag="PXD81_001985"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005604148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HDL5537979.1"
                     /translation="MEKHHIETLHSKEEVLNRINELAKEINQHYHQKQCQNLVVVGLL
                     RGSFMFMADLVRKLELPVEVDFLTASSYGSSTESSRDVKILKDLDGEIQGKDVLIVED
                     IIDTGFTLSKVGEILKLREPNSLAICTLLDKPCRREVDVPVKWVGFEIPDEFVVGYGI
                     DYAQKYRHLDYIAKVVIE"
     gene            complement(587..1936)
                     /gene="pmbA"
                     /locus_tag="PXD81_001986"
     CDS             complement(587..1936)
                     /gene="pmbA"
                     /locus_tag="PXD81_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HDL5537980.1"
                     /translation="MSITDKQTLLQQEQELRQAVEFALNFAKQAGAEAEVGVTKVAGL
                     SVSSRLEQVENIEFNNDGSLGISVYLGKRKGNASTSDLQPESIQKTVEAALAIAKYTS
                     EDACTGLADKELLAFEAEDLELYHQADISVDQAVELAIEAEHYALNADEKIVNSDGAT
                     FNSHSGVRVYGNTHGMLQSYLSSRYSLSCSVISAYEDQLERDYEYTIFREFDKLQAPK
                     WVGEQAAIKAVDRLNPQRIKTGEMSVIFYNDVATGLVGHLAGAISGGALYRKSSFLLD
                     KLGEQILPEWFAISERPHLLRQLASSAFDSEGVITQNREIITDGVLQTYLMTSYSGRK
                     MGLKSTGHAGGIHNWLVKPNRQGGLNTLLKEMGTGLLVTEMLGSAINSVTGEYSRGAA
                     GFWVENGEIQYPVAEITIAGQLQDMYKNLVAVGDDIEHRSNIQTGSILLEKMMVSGE"
     gene            2035..2565
                     /locus_tag="PXD81_001987"
     CDS             2035..2565
                     /locus_tag="PXD81_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005604242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein"
                     /protein_id="HDL5537981.1"
                     /translation="MAKKRSKNEIDWTDEEEEIIWVSKSEIKRDSEHLKKIGAELIEL
                     TPQNLEKIPLDDDLKEAIRQAQGFKMEARRRQIQFIGKLLRNRDQEPIQEALDKVKNR
                     HNQQQALLHKLELVRNQLIAMGDASLDNLLTEYPQLDRQHLRNLIRGAIKEREANKPA
                     KNYREIYQYLKTEIVE"
     gene            2571..2942
                     /locus_tag="PXD81_001988"
     CDS             2571..2942
                     /locus_tag="PXD81_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB2 family protein"
                     /protein_id="HDL5537982.1"
                     /translation="MEYLSTILKAHIGLAYISLILLLTRGFLSSKMVDWRQYKILRIA
                     PHAVDTFLLITGIAAVAIFLAYDFFTLAQFSWLLPKLLFLVLYIVFATKAFKKGQPFS
                     LKFYLLAVVSFLLMMLTATFH"
     gene            2955..3668
                     /locus_tag="PXD81_001989"
     CDS             2955..3668
                     /locus_tag="PXD81_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HDL5537983.1"
                     /translation="MIGPFVNAGAIILGGVLGACLGSHLPERIRTTLPLVFGLCCFGL
                     GMMLTASVKYMSAVALALILGTIIGELIYLENRIGKVAGLARGLIDRLFPPVHSLSQE
                     EFLEKFIALLVLFCVSGTGIFGAIKEGMTGDPSILYIKSILDFFTAMIFAASLGYAIA
                     AIAVPLIIVQGILILLATVIMPLTTANMMADFSATGGLLLFATGFRICGIKNFPVANM
                     LPALLLAMPMSYFWEWLVH"
     gene            3671..4234
                     /locus_tag="PXD81_001990"
     CDS             3671..4234
                     /locus_tag="PXD81_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HDL5537984.1"
                     /translation="MKKRIIVGISGASGFQYGYKVLELLKPFSEIETHLVISKGAELT
                     RTLETDYTREALHDLADQIHSLNNVGASIASGSFKTEGMVIAPCSMRTLAAIANGLSD
                     NLLTRAADVVLKERRKLVLMVREAPLNLAHIDNMRKVTEMGGIIFPPIPAFYQKPTSL
                     DEMVTHSVSHALSLLDIDIPNTPHWGE"
     gene            4297..4737
                     /locus_tag="PXD81_001991"
     CDS             4297..4737
                     /locus_tag="PXD81_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HDL5537985.1"
                     /translation="MHNPIPTHILDFIQTNHVVNIAAHTEQDFWAANCFYAFDQENVR
                     LIILTAKKTRHAGLMLENPHIVGTICGQIEKINEIEGVQFSATAECLEDKQAEKAALQ
                     IYYQKHPLARLKPSDVWVLSFNTIKHTGNKLMFAKKTIWERNII"
     gene            complement(4741..>5583)
                     /locus_tag="PXD81_001992"
     CDS             complement(4741..>5583)
                     /locus_tag="PXD81_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL5537986.1"
                     /translation="QIKGFSHLYRLRSDLMLKYTEISVTETVEQEPAEFEAGLRLVIL
                     LSGRSEIDFNSEVFELDASLKPQAAFLPLNRAEMGRKIFRKGQLQKELVIFLYPKWLI
                     ESQLNLASYHHFKQHLKAHQIILTQSMLKLIHDIVSEQEMQPFGLLEKEGNLLALLAS
                     AFSQLTQAPRLKKDRVQQLTELLESGKFDGWSLKQIAIYFHTNVTTLQNEFQHYHKTS
                     IFAYLRRSKLEQAYKLLLQGQSITQAAEFAGYANSENFSTAFKRTFGITPSQIKRDKA
                     KGII"
BASE COUNT         1608 a         1184 c         1050 g         1741 t
ORIGIN      
        1 attgagtttt tttattcaat cactacttta gcaatataat ctaaatgacg atatttttgg
       61 gcataatcaa tcccataacc caccacaaat tcatcgggga tctcaaatcc cacccatttt
      121 acgggcacat caacttcgcg gcggcaaggt ttatctaata aagtacaaat agcaagtgaa
      181 tttggctcac gtaacttgag aatctcccct accttgctta aggtgaagcc agtatcaata
      241 atatcttcta caatcaatac atctttacct tgaatttcgc catctaaatc ttttagaatt
      301 ttgacatcac ggctggattc cgtgctgctg ccatagcttg atgcggttag aaaatccact
      361 tctacaggta actctagctt tcttactaaa tctgccataa acataaacga gcctcgcaat
      421 aaaccaacca ctacaaggtt ttggcactgt ttttggtgat agtgttgatt gatttctttt
      481 gctaattcat tgattcggtt taatacttct tcttttgaat gtagcgtttc gatatgatgt
      541 ttttccattg atttctccaa ataaaattga ttacaagcgg ccgtttttac tcgcctgaaa
      601 ccatcatttt ttctaataag attgaacctg tttggatatt tgagcggtgt tcaatatcat
      661 cgcctaccgc cactaaattt ttatacatat cttgcagttg ccctgcaatg gtaatttccg
      721 ctacagggta ttgaatttca ccgttttcta cccaaaatcc tgctgcacct cgagagtatt
      781 cccctgttac gctattaatc gcagaaccta gcatttccgt tactaataat cctgttccca
      841 tttccttaag taacgtattt aaaccacctt gacgatttgg tttaactaac cagttatgaa
      901 taccacctgc gtgtcctgta ctttttaacc ccattttacg ccctgaatag ctagtcatta
      961 aatacgtttg tagcacgcca tcagtaataa tttcacggtt ttgggtaatt acaccttcac
     1021 tatcaaacgc agaagaggct aattgacgca acaaatgcgg acgctctgaa atcgcaaacc
     1081 attcaggcag aatctgttcg cctaatttat ctaataagaa acttgatttg cggtataatg
     1141 caccgccact aatagctccg gccaaatgcc ccactaaacc ggttgccaca tcgttataga
     1201 aaatcacaga catttcgccg gtcttaattc gttgcggatt caatctatct accgctttaa
     1261 ttgcagcttg ctcccctacc catttcggtg cttgtaattt atcaaactca cggaaaatgg
     1321 tgtattcata atcacgctca agctgatctt cataagctga aatcacgcta caagagagtg
     1381 aatatcggct cgacaggtaa ctctgcaaca ttccgtgcgt attgccataa acacgcacgc
     1441 cactgtgaga attgaaagtt gccccgtcac tattcacaat tttttcgtct gcatttaagg
     1501 cataatgctc cgcttcaatc gccaattcga ctgcttgatc aactgaaata tccgcttggt
     1561 ggtacaattc caaatcttct gcctcaaagg caagcaattc tttatcagca agcccggtac
     1621 aagcatcttc tgaggtatat tttgcaatcg caagagctgc ttcaaccgtt ttttgaatcg
     1681 actctggctg aagatcggag gtggaagcat tacctttgcg tttgcctaaa taaaccgaaa
     1741 taccgagaga accatcgtta ttgaactcga tgttttcaac ttgctctaaa cggcttgaaa
     1801 ccgaaagccc ggcaacttta gtcacgccta cttccgcctc tgccccagcc tgttttgcaa
     1861 aatttaaggc aaattcgacc gcttgtcgta gctcctgttc ttgttgtaac aaggtttgtt
     1921 tatctgtaat tgacattttg ttccattgat tgattgcatt gatgaatata ttctaactca
     1981 aatttctgtt agaatacggc attttttaga gtttatgtaa tgaaaggata aagaatggct
     2041 aaaaaacgga gcaaaaacga aattgactgg acagatgagg aagaagaaat catctgggtc
     2101 agcaaaagtg agataaaacg agattctgaa cacttaaaaa aaattggggc tgaattgatc
     2161 gaattgaccc cacaaaattt ggaaaaaatt ccactagacg atgatttaaa agaggcaatt
     2221 cgccaagctc aaggctttaa aatggaggct cgccgccgtc aaattcaatt tatcggcaaa
     2281 ttattacgca accgtgacca ggagccgatt caagaagcct tagataaagt gaaaaatcgc
     2341 cataatcagc aacaagccct attacataaa ttggaattag tgcgtaatca actgattgca
     2401 atgggcgatg catcattaga taatttattg acggaatatc ctcaattaga ccgccaacat
     2461 ttgcgtaatt taattcgtgg ggcaattaaa gagcgtgaag ccaataaacc ggcaaaaaat
     2521 taccgtgaaa tttatcaata tttaaaaact gaaatcgtgg agtgattatt atggagtacc
     2581 tatcaaccat tcttaaagct catatcggct tagcttatat tagtttaatt ttattattaa
     2641 cccgtggatt tttatcgtca aagatggtgg attggcgaca atataaaatt ttgcgcattg
     2701 ccccacacgc agttgatacc ttcttactta tcaccggtat tgctgctgtc gctattttct
     2761 tagcttatga ttttttcacc ttagcacaat tcagttggtt gctaccaaag ttactcttct
     2821 tagtacttta tattgtattt gcgacaaaag cgttcaaaaa aggacaaccg ttttcgctca
     2881 aattttatct gttagccgta gtctcattct tattaatgat gctaactgcc acttttcact
     2941 aaataaggga aactatgatc gggccttttg tgaatgccgg tgcaattatt ttagggggcg
     3001 tgctgggtgc ttgtttaggg agtcacctgc ctgaacgtat tcgcactacg ttgccgctgg
     3061 tattcggctt atgctgtttc ggcttaggta tgatgctgac tgcctcagta aaatatatgt
     3121 ctgcggttgc cttagcattg attttaggca cgatcatcgg tgaactgatt tatttagaaa
     3181 acagaatcgg taaagttgcc ggtttagctc gtggtttaat tgaccgccta ttcccaccgg
     3241 tgcatagtct ttcccaagaa gagtttttag agaaatttat tgctctgctt gtactgtttt
     3301 gtgtgagcgg taccggcatt tttggtgcaa taaaggaagg aatgacaggc gatccaagta
     3361 ttctttacat taaatcgatt ctagatttct tcaccgcaat gattttcgct gcttcgttag
     3421 gctatgccat tgcggcgatt gcagtgccgt tgattattgt gcaagggatt ttaattctac
     3481 tggcaaccgt gattatgcct ttaaccactg ccaatatgat ggcagatttc tccgcaaccg
     3541 gcggtttact gctatttgca accggctttc gcatttgtgg aattaaaaac tttccggtcg
     3601 caaatatgct accagcacta ctccttgcga tgccgatgtc ttatttctgg gaatggttgg
     3661 tacattaatg atgaaaaaac gtattattgt cggcattagt ggggcaagtg gttttcaata
     3721 tggctataaa gtgcttgaac tgctgaaacc gttttcagaa attgaaaccc accttgtaat
     3781 cagcaaagga gctgaactca ctcgtactct cgaaacggat tatacccgag aagctcttca
     3841 tgatttagct gatcaaatac attcgctaaa caatgtcgga gcaagcattg ccagtggttc
     3901 atttaaaacc gaaggtatgg tcatcgcccc gtgttctatg cgaacccttg ccgctattgc
     3961 caacggtttg agcgataatt tgctgacccg tgcggcagat gtggtgttga aagaacggcg
     4021 taaattggta ttaatggtga gagaagctcc gctcaacctt gctcatattg acaatatgcg
     4081 aaaagtcaca gaaatgggcg gcattatttt cccacccatt ccggcgtttt accaaaagcc
     4141 aaccagcctt gacgagatgg tcacccacag cgtaagccat gccctttccc tgttggatat
     4201 tgacattcca aataccccac attggggaga ataattcggc ttatcttttg ctttttgaaa
     4261 tgctagaata cctgataaaa actatcaggt atttttatgc acaatccaat cccgacccat
     4321 attttagatt tcattcaaac taaccacgtt gtcaatattg cagctcatac cgaacaggat
     4381 ttttgggcag caaattgctt ttatgccttt gaccaagaaa atgttcgctt gattatctta
     4441 accgccaaga aaaccagaca cgctggcttg atgcttgaaa acccacatat tgtcggcaca
     4501 atttgtgggc aaattgagaa gatcaacgaa attgaaggtg tccaattctc cgctaccgcc
     4561 gaatgtttag aagataaaca agccgaaaaa gccgctttgc aaatttatta ccaaaaacac
     4621 ccgcttgcac ggcttaagcc tagcgatgtt tgggtgttat cattcaatac catcaagcac
     4681 accggtaata aacttatgtt tgcgaaaaag acgatttggg agagaaatat aatttgaagc
     4741 tcaaataatg cctttagcct tatcccgttt gatttggctt ggtgttatcc caaaagttcg
     4801 cttaaatgcc gttgaaaaat tctccgaatt ggcatatccg gcaaattcag ctgcttgtgt
     4861 aatactctgc ccttgtaata gtaatttata ggcttgttct aatttagaac gccttaaata
     4921 cgcaaaaata gaggttttgt ggtaatgctg aaattcattt tgtaatgtgg tcacattggt
     4981 atggaaatat attgcaatct gcttcaaact ccagccgtca aatttaccgc tctcaagcaa
     5041 ttctgttaac tgttgcaccc gatctttttt taaccgggga gcctgtgtca gctgagaaaa
     5101 agcacttgct aataaagcta atagattacc ttctttctca agtaacccaa atggttgcat
     5161 ttcctgctct gacacaatat cgtgaataag ttttaacata ctctgagtca aaataatttg
     5221 gtgggctttt aaatgctgtt taaagtgatg atagcttgcc aaatttaact ggctttctat
     5281 tagccattta gggtagagaa agataacaag ctctttttga agttgccctt tcctaaaaat
     5341 tttacgcccc atttcagctc tatttagcgg taaaaatgcg gcttgaggtt ttaaacttgc
     5401 atctaattca aatacttccg aattaaaatc aatttccgat cgaccactca acaatataac
     5461 caatcttagt ccggcttcaa attccgcagg ttcttgctct acggtttcag taactgaaat
     5521 ctctgtatat ttcagcataa gatctgaacg tagtcggtat aaatgtgaaa aaccttttat
     5581 ttg
//
LOCUS       DANLYI010000158         5507 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.159, whole genome shotgun sequence.
ACCESSION   DANLYI010000158 DANLYI010000000
VERSION     DANLYI010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 5507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5507
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.159"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            complement(107..622)
                     /locus_tag="PXD81_001993"
     CDS             complement(107..622)
                     /locus_tag="PXD81_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDL5537987.1"
                     /translation="MAGINKVIIVGNLGNDPEMRTMPNGEAVANISVATSESWTDKNT
                     GERREVTEWHRIVFYRRQAEVCGQYLRKGSQVYVEGRLKTRKWQDQNGQDRYTTEIQG
                     DVLQMLGSRQGNDMGGQQGGWGNSAPAGNSYNQGNSNYGYNQTQSAAPQSKPAPQPEP
                     AMNNFDDDIPF"
     gene            777..3605
                     /gene="uvrA"
                     /locus_tag="PXD81_001994"
     CDS             777..3605
                     /gene="uvrA"
                     /locus_tag="PXD81_001994"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005612043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HDL5537988.1"
                     /translation="MQHIDIRGARTHNLKNINLTLPRDKFIVITGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTVT
                     EIHDYLRLLFARVGEPRCPDHDVPLAAQTISQMVDRVLEEPEGKRLMLLAPVVKERKG
                     EHVKLLENLTASGYLRARIDGEICDLSDPPALELQKKHTIEVVVDRFKVRADLATRLA
                     ESFETALDLSGSTAIIADMDDPNAEEIVFSSSFACPHCGYSLHELEPRLFSFNNPAGA
                     CPTCDGLGVEQFFDAKKVVQNEEVSLAAGAIKGWDRRSFYYFGLLKAVAEHYGFDLET
                     PFGQLPKKYQNIILNGTKEEIEFVYVNDRGDKVKRKHAFEGVLNNMARRYKETESNSV
                     REELAKYINNRPCMDCEGSRLRREARYVFLDKTNLPMVSEKSIGEALTFFEELKLAGQ
                     KEKIAEKILKEIRERLQFLVNVGLNYLSLSRSAETLSGGEAQRIRLASQIGAGLVGVM
                     YVLDEPSIGLHQRDNERLLNTLVHLRNLGNTVIVVEHDEDAIRAADHIVDIGPGAGVH
                     GGQVIAQGTAAEIMQIEESITGQFLSGKQKIEIPAQRVPYEPTKLLTLSGASGNNLKK
                     VKLEIPVGLFTCITGVSGSGKSTLVNDTLFPLAQNALNRAENSDVAPYESIDGLSHFD
                     KVIDIDQSPIGRTPRSNPATYTGLFTPIREIFAGTQEARARGYNVGRFSFNVRGGRCE
                     ACQGDGVIKVEMHFLPDVYVPCDHCKGKRYNRETLEIRYKGKTIHQVLEMTVEEAREF
                     FAPMPMIARKLQTLMDVGLSYIRLGQSSTTLSGGEAQRVKLATELSKRDTGKTLYILD
                     EPTTGLHFADIKQLLGVLHRLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGEI
                     IATGTPEEVAKDKRSHTARFLKEILAKG"
     gene            complement(3697..5430)
                     /gene="dauA"
                     /locus_tag="PXD81_001995"
     CDS             complement(3697..5430)
                     /gene="dauA"
                     /locus_tag="PXD81_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HDL5537989.1"
                     /translation="MLNKWFLTKNAFLAVKPFSALKDALNAGYGKQHLIRDIISGLTV
                     GVIAIPLSMALAIASGVPPQHGLYTAIVAGIVIALTGGSRFNISGPTAAFVVILYPVT
                     QQFGLSGLLMATMLSGGILVLMALLRLGRLIEYIPLPVTLGFTCGIGIVIATLQIKDF
                     LGLSIEKMPEHYLGKLEAIFSALPTINWADTLVGVVTLLVLTRWHKLRLPIPGHLPAV
                     FIGTLLSLLLIHFGQNVATIGSAFHYTLSDGTTGYGIPSVLPEFVLPWNLPNAEGNRI
                     DWNLTTIQALLPAAFSMAMLGAIESLLCAVVCDNMTDTKHHSNNELLAQGLGNIASPF
                     LGGITATAAIARSAANVRSGAVSPISSVVHALLVLFALLFLAKALSYLPLSSMAALLL
                     VVAWNMADIPQIIQLTRRSGKNEIAVLLTCITLTVLFDMVIAITVGVLLASLLFIRTI
                     AEMTKSFEMAHPTDLNDVLVYRISGPLFFAAADNLFADLHNKTVHTDHQIKHIVLQCD
                     AVTVLDAGGIHALTRFVQHMLPHQQLHLCNVQFQPMRMLVRSQKTVNEIPQIQFSSDL
                     ESAFDKIRQAN"
BASE COUNT         1587 a         1148 c         1287 g         1485 t
ORIGIN      
        1 gttccctatt attttgagta aggtaggggc gctttatatg agtagctttt tatttcatcg
       61 aaatgcggtg tatggaaccc atgagctact taagtcaata ctttgattag aacggaatat
      121 catcatcaaa attgttcatt gccggctcag gctgtggtgc cggtttagat tgcggtgcag
      181 cactttgggt ttggttgtaa ccataattgc tgttgccttg attgtagcta ttacccgcag
      241 gagcagaatt tccccaaccg ccttgctgac cgcccatatc attaccttga cggctgccga
      301 gcatttgtag cacatcgcct tgaatttcgg tggtgtaacg atcttgcccg ttttggtctt
      361 gccatttacg ggtttttaaa cgtccttcta cgtaaacttg agagccttta cgtaaatatt
      421 gcccgcatac ttctgcttga cggcggtaga atacaatacg atgccactcg gttacttcac
      481 ggcgttcacc ggtgttttta tccgtccagc tttctgaggt tgccacacta atatttgcaa
      541 ccgcttcgcc atttggcatt gtacgcattt cagggtcgtt acctaaatta ccgacaataa
      601 ttactttatt gataccagcc ataaaatcct ctgtttgata aattaaacta aaatttgcta
      661 agtattctac ctgaaaatcc aaaagatgaa caccaaaaaa ctgatatttt gtacagtttt
      721 attaaaatat gcgataatga tcgaaaattg agaaaatagc actggacatt aaaaatatgc
      781 aacatatcga tattcgtgga gcaagaacac acaacttaaa aaacatcaat ttaaccttgc
      841 cgagagataa attcattgta attaccgggc tttccggctc gggcaaatcg tctctggcgt
      901 ttgatacgct ctatgcggaa ggacaacgcc gttatgtaga atcactgtct gcatacgccc
      961 gccagtttct ctccttaatg gaaaagcctg atgtggatca tattgaaggg ctttcccctg
     1021 cgatttcgat tgagcaaaaa tcaacctctc acaacccacg ttcgaccgta ggaacggtaa
     1081 cggaaattca cgactattta cgtttattgt ttgctcgagt gggcgagcct cgttgccctg
     1141 accacgatgt gccattggct gctcaaacta tttcgcagat ggtggatcga gtattggaag
     1201 aaccggaagg aaaacggttg atgctacttg cccctgtggt gaaagagcgt aaaggcgaac
     1261 acgtaaaatt attggaaaat ttgaccgcta gtggctattt gcgggctaga attgacggtg
     1321 aaatttgcga tttatccgac ccaccggcat tagaattaca gaaaaaacac accattgaag
     1381 tggtggtgga tcgctttaaa gtaagggctg atctagccac ccgtttggcg gaatcttttg
     1441 aaacagcgtt agatttatcc ggctctaccg caattattgc agatatggac gacccaaatg
     1501 ccgaagaaat tgtgttttcg tccagctttg cttgtccgca ttgtggttac tctttacacg
     1561 aattagaacc acgccttttc tcgtttaaca acccggcagg tgcttgcccg acttgtgatg
     1621 gtttgggcgt tgagcagttt tttgatgcca aaaaagtagt gcaaaatgaa gaggtttcat
     1681 tagccgccgg tgcgatcaaa ggctgggatc gccgcagttt ctactatttt ggcttattaa
     1741 aagccgttgc cgagcattat ggctttgatt tagaaacccc attcgggcag ctaccgaaaa
     1801 aatatcaaaa cattatttta aacggcacta aagaagagat tgagtttgtt tatgtcaatg
     1861 accgaggcga taaggtcaaa cgtaaacacg cttttgaagg ggtgctgaat aatatggctc
     1921 gccgctataa agaaaccgaa tcaaactcgg tgcgtgaaga gttggcgaag tatattaata
     1981 atcgtccttg tatggattgc gaaggctcac ggttacgccg tgaagcccgt tatgtattct
     2041 tagataaaac caatttgccg atggtttcgg aaaaaagtat cggcgaggca ttaacctttt
     2101 ttgaagagct gaaattagcc ggtcagaaag aaaaaattgc tgagaaaatc ctcaaagaaa
     2161 ttcgtgaacg gctacagttt ttagtgaatg tcggcttgaa ttatctttct ctttcccgct
     2221 cggcagaaac cctttcaggt ggcgaggctc agcggattcg ccttgccagc caaattggtg
     2281 cgggtttagt aggggtaatg tatgtgctgg atgagccttc catcggcttg caccaacgag
     2341 acaatgaacg cctactgaat actttggtgc atttacgcaa tttaggcaat accgtgattg
     2401 tggttgaaca cgatgaagat gcgatccgag ccgcagatca tatcgtggat ataggcccgg
     2461 gtgccggtgt acacggtggg caagttatcg cacaaggtac ggcagctgaa attatgcaaa
     2521 ttgaggaatc tattaccgga caattcctct caggcaagca aaaaattgag attccggctc
     2581 agcgtgtgcc ttatgagcca actaaattgc tcaccttatc gggagcaagt ggtaacaact
     2641 tgaaaaaagt gaaattagag attccggttg gtttatttac ttgtatcacg ggtgtatcag
     2701 gttcgggcaa atcaacttta gttaatgaca cactattccc tctggcacag aatgcgttaa
     2761 accgagcgga aaacagcgat gttgctcctt atgaatcgat tgacggctta tcgcattttg
     2821 acaaggtaat tgatattgac caaagcccga ttgggagaac tcctcgctca aatccggcaa
     2881 cttacaccgg attatttacg ccaattcgtg agatttttgc cggcacgcaa gaagctcgag
     2941 ccagagggta taacgtaggg cggtttagct ttaatgtgcg gggcggacgt tgcgaagcct
     3001 gtcaaggtga cggcgtgatt aaagttgaaa tgcacttctt gcctgatgtg tatgtgcctt
     3061 gcgatcactg taaaggcaaa cgctataacc gtgaaacctt ggaaattcgc tataaaggca
     3121 aaactattca ccaagtgtta gagatgacgg tggaagaggc gagagaattt tttgccccta
     3181 tgccgatgat tgcccgcaag ttgcaaacct tgatggacgt ggggttatct tatatccgtt
     3241 tagggcaatc ttctactacg ctctcaggcg gtgaagccca acgggtgaaa ttggcgaccg
     3301 agctttccaa acgagacacc ggcaaaacgc tctatatttt agatgaaccg accaccggct
     3361 tgcattttgc cgatattaaa cagttactcg gcgtgctaca tcgcctgcgt gatcaaggca
     3421 atacgattgt ggtgatcgaa cataatttag atgtgattaa aaccgcagac tggattgtcg
     3481 atcttggccc tgaaggtggc agtggcggcg gtgaaattat tgctaccggc acaccggaag
     3541 aagtggcaaa agacaaacgt tctcacaccg cacgcttctt aaaagagatt ttggctaaag
     3601 gctaacgttc aacacaagcg gtcatatttt cggaaaatgt tgcaatttgc aaaaagttgg
     3661 aaaatatgac cgcttgtttt atatccggag ttattcctag ttagcctgtc tgattttgtc
     3721 gaaggcagat tcaagatcgc tactgaattg aatttgcggg atttcgttta ccgttttttg
     3781 tgagcggact agcattctca tcggctgaaa ttgcacatta cagagatgaa gttgttgatg
     3841 tggcaacata tgctgtacaa agcgggttag ggcgtgaatg ccaccagcat ctaatactgt
     3901 aacggcatcg cattgcagca caatatgttt aatttggtga tcggtatgta cggttttatt
     3961 gtgcagatcg gcaaagagat tatcggcggc agcaaaaaat aacggaccgc tgatccgata
     4021 aaccaacaca tcgttcaaat cggttgggtg agccatttca aaggatttag tcatttctgc
     4081 aatagtacgg ataaatagca ggcttgccag taacacccct accgtaatgg caattaccat
     4141 atcaaataat accgtgaggg taatgcaagt cagtaaaacc gcaatttcat ttttacctga
     4201 acggcgggta agttgaatga tttgtggaat atctgccata ttccaagcta ctaccaataa
     4261 tagtgcagcc attgaggaaa gaggcaaata ggaaagtgct ttagccaaaa agagtagggc
     4321 aaataaaacc aacaaggcgt gaaccacact agaaatgggg gaaactgccc cggaacgaac
     4381 attggcagcc gatctcgcaa tcgctgctgt ggcggtaatt ccccctaaaa atggcgaggc
     4441 aatattacct aagccttggg ctaatagttc gttgttagag tggtgcttgg tatctgtcat
     4501 attatcgcac actaccgcac ataataacga ttcgatagcc ccaagcattg ccattgaaaa
     4561 ggcagccggt aataaggctt gaatggtggt taagttccaa tctatccgat tcccctctgc
     4621 attcgggaga ttccaaggta acacaaattc aggtaatacg cttgggatac catacccagt
     4681 ggtgccgtca gagagcgtgt agtggaatgc tgagccgata gtggcaacgt tttgtccgaa
     4741 atgaatcagc agtaatgata acagcgtgcc gataaagacg gcgggtaaat gcccgggaat
     4801 aggcaaccgt agcttgtgcc aacgagttaa aaccaacaat gttacgacac ccactaaggt
     4861 gtcagcccaa tttatggttg gcaatgctga aaaaatagcc tctagcttgc caagatagtg
     4921 ttcaggcatt ttttcaatgc ttaagcccag aaaatctttg atttgcaagg tggcgatcac
     4981 aataccgatg ccacaggtaa aacccagtgt gacaggtaga ggaatgtatt caattaagcg
     5041 tcctaatcgg agtaacgcca tcaatactaa aattcctccg gaaagcattg ttgccatgag
     5101 taatccactt aagccaaact gctgcgtgac ggggtataga atcaccacaa aagcggctgt
     5161 tgggcctgaa atattaaatc ttgaaccgcc tgttagggca atcacaatcc cggcaacaat
     5221 cgccgtatat aaaccgtgct gtggtggcac accgctggca atcgccaacg ccatagataa
     5281 cggaatagca ataacgccaa cggttaagcc tgaaataata tctctgatta aatgttgttt
     5341 accataacct gcattgagag catcttttaa tgcactaaaa ggcttgacgg caagaaaagc
     5401 atttttagtt aaaaaccatt tatttaacat agaatgttcg agtgaagaga atgtttcgct
     5461 tattttactg caatgaataa aattaaattt gagctaacgc aagaaaa
//
LOCUS       DANLYI010000159         5442 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.160, whole genome shotgun sequence.
ACCESSION   DANLYI010000159 DANLYI010000000
VERSION     DANLYI010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 5442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5442
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.160"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            108..626
                     /gene="hutZ"
                     /locus_tag="PXD81_001996"
     CDS             108..626
                     /gene="hutZ"
                     /locus_tag="PXD81_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme utilization protein HutZ"
                     /protein_id="HDL5537990.1"
                     /translation="MTTNRQEVLQNRLGPEIEELKQQIKTIMLATIDKDGVPNVSYAP
                     FVINNGEYQVLITTIARHARNLQEVPKVSLMLIEDESKSRQIFARRRLTFDATVRMIE
                     RTDEEWNNSIAALKARHGDLVDELSNMQDFKLFSFKPTAGLFVKGFGKAFDVGVDDLI
                     NVVHLDQGHQTA"
     gene            776..3388
                     /gene="pepN"
                     /locus_tag="PXD81_001997"
     CDS             776..3388
                     /gene="pepN"
                     /locus_tag="PXD81_001997"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HDL5537991.1"
                     /translation="MQPKAKLRKDYRAPDFTITDIYLDFQLDPDRTFVTSKLTVVRKN
                     PEATTLRLDGHSFDFLSLKYNGEPFSAFQKDDESLTLNLADVPAERFELEIETALNPA
                     QNTSLQGLYKSGDGFCTQCEAEGFRQITYMLDRPDVLAKYTTKITASKSKYPYLLSNG
                     NHIAQGDLHDGRHWVEWQDPFFKPSYLFALVAGDFDLLQDKFITKSGREVTLEIYVDR
                     GNLDRAPWAMESLKRSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKF
                     VLAKPETATDTDYLDIEAVIAHEYFHNWTGNRITCRDWFQLSLKEGLTVFRDQEFTSD
                     LWSRSAKRIEDVRLLRTVQFAEDASPMAHPIRPEKVIEMNNFYTVTVYEKGAEIIRMI
                     HTLLGEEKFQQGMQLYVARHDGSAATCEDFVKAMEDASGVNLEQFRRWYSQSGTPELT
                     ISDEYDETRKAYRLHISQMTPATHDQMDKLNLHIPLKVALYGEQDGKRIPLQYELLTV
                     SDVLDVTAEHQTFEFHNVTQRPVPALLCDFSAPVRLDYNYTTEQLLTLLKHAENDFVR
                     WDAAQMLYTNELRENLSRYQQHQPLSFSEGLVNALTFVLDNSASLPELTALTLTLPKE
                     TEFAESFKTIDPQGIAVVREFMQHSIAHALQDKLLAVYHQNQCGEYQVVAEDIAKRAL
                     RNACLSYLAFTDLGNALVHKHYNNADNMTDTLAALQAATKAQLSCRDALLADFEQKWK
                     HDGLVMDKWFMLQATRPDDDVLSIVQNLMEHPSFNFNNPNRLRSLVGAFCGQNPKAFH
                     AIDGSGYRFLVDTLIKLNESNPQVAARLIEPLIKLSRYDNQRQTLMRRGLERLRELDN
                     LARDLFEKIEKALD"
     gene            complement(3448..3720)
                     /locus_tag="PXD81_001998"
     CDS             complement(3448..3720)
                     /locus_tag="PXD81_001998"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HDL5537992.1"
                     /translation="MVAKLFSVYGRVQGVAFRFFTLQEANRLGIKGYVRNRDDGSVEV
                     VAEADEQTIAQLENWLQQGSPAAKVERVIVSDYLGSERFKRFEVRY"
     gene            complement(3774..5408)
                     /locus_tag="PXD81_001999"
     CDS             complement(3774..5408)
                     /locus_tag="PXD81_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005700110.1"
                     /note="is involved in the transport of the murein peptide
                     L-alanyl-gamma-D-glutamyl-meso-diaminopimelate; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HDL5537993.1"
                     /translation="MQSKFSLSLLTTSLVLGLSANVFAAKVPEGTTLAEKQEITINNG
                     FEPSSFDPHKIEGVPESQVAYQLFEGLVTKDSDGNIQPGVAESWENTPDYKTWTFKLR
                     QDAKWSNGDAVTAHDFVFAWQRLVNPLTASPYSSYLTYLNVENAQDIIDGKKKPEELA
                     VKAIDDHTFQVQLSQPVPYAVELATHSSLLPVHKATVEKHGDAWVQKGNLVGNGAYTL
                     ADHVINEKIVFERNKNYWNDKETVINKATFLAIANAATDVSRYRAGDLDMTNYALPPE
                     QFATLKKEIPNEVFTARTLSTYYYEINHKRAPFDNVKVREALNLALDRTIITDKVLGQ
                     GQTPTYVFTPPYIHEGEKIQQPAYSKDSMADRKAKALKLLEEAGYSKANPLKFTILYN
                     TNDNHKKIAIAVSSLWKQGTDGIVDVKLENQEWKTFLDTRRNANYDVSRAGWSADYNQ
                     ATTFVNYFLSNSSNNTAFYKSQAYDDAVAESYKATDAAGRAAAYAKAEEQLAKDYAII
                     PIYNYVNPRLVKPYVKGYEGKDPQDDILLRNLYIVK"
BASE COUNT         1587 a         1171 c         1083 g         1601 t
ORIGIN      
        1 tattatcagt tacatcacat ttatagtcaa taatgattct catatggatt gaaagttttc
       61 actctttata gtaatctaaa aaaacactat ttaaattgag gtattttatg acaaccaacc
      121 gtcaagaggt cttacaaaac cgtttaggtc cagaaattga agaattaaaa caacaaatca
      181 aaactataat gcttgcaacc atagataaag atggtgtacc gaatgtgagc tatgcccctt
      241 ttgtaattaa taacggcgaa tatcaagtct taatcactac tattgcccgt cacgctcgta
      301 atttacaaga ggtgccaaaa gtctctttaa tgctaattga ggatgaaagt aagagccgtc
      361 aaatttttgc ccgtcgtcgc ttaacctttg acgcaacagt gcgaatgatt gaacgtactg
      421 atgaggaatg gaataatagc attgcagcac ttaaagctcg tcacggcgat ttagttgatg
      481 aactgtcaaa tatgcaagat ttcaagctct ttagctttaa gccaacggca ggcttatttg
      541 taaaaggatt tggtaaagcc tttgatgttg gtgttgatga cttaattaat gtcgttcatt
      601 tagaccaagg ccaccaaact gcctaattgc gaaataaact cacggctata aataatccta
      661 tttatagccg tttttattgc gacaagcggt caaatttttt ggaaaattta ccgctatttt
      721 agtacaataa cgacactatt ctatgagcta aacgcaacta aaaaaaggaa atcctatgca
      781 acctaaagcg aaactcagaa aagattatcg agctcccgat ttcactatta ctgatattta
      841 tcttgacttc caacttgacc cagatcgcac ttttgtcacc tcaaaactaa ctgtcgtgcg
      901 taaaaatcct gaggcgacaa cacttcgttt agacggtcat agtttcgatt tcctttcgct
      961 taaatataac ggcgagccgt tttccgcctt tcaaaaagat gatgaatctt taaccttaaa
     1021 tttagccgat gtgcctgccg agcggttcga gcttgaaatt gaaaccgcat taaatccggc
     1081 acaaaacacc tcgttacaag gtttgtataa atcgggcgac gggttttgca cccagtgtga
     1141 ggctgaaggt ttccgccaga ttacctatat gctcgaccgc cctgatgtac ttgcaaaata
     1201 tacgacaaaa atcaccgcta gtaagagcaa atacccgtat ctgctttcta acggcaacca
     1261 cattgcccaa ggcgacttgc acgatggcag acactgggtg gaatggcaag atccgttctt
     1321 taaaccaagc tatttatttg ccttggttgc gggggatttt gacttactcc aagataaatt
     1381 catcaccaaa agcggacgtg aagtcacgct tgaaatttat gtcgatcgtg gaaatttaga
     1441 ccgtgcacct tgggcgatgg agagcttaaa acgttcaatg aaatgggatg aagagcgttt
     1501 cggtttggaa tacgatttag atatttatat gattgttgcc gttgatttct tcaatatggg
     1561 agcaatggaa aacaaagggt taaatatttt caattccaaa tttgtattgg caaaaccgga
     1621 aaccgcaacc gatacggatt atttagatat tgaagcggtg attgcccacg aatacttcca
     1681 taattggacg ggcaaccgca ttacctgccg tgattggttc caactaagct taaaagaggg
     1741 cttaaccgtc ttccgtgatc aggaatttac ctccgattta tggtctcgtt cggcaaaacg
     1801 gattgaagat gtgcgtttac tgcgtaccgt acaatttgcc gaagatgcca gcccgatggc
     1861 tcacccgatt cgcccggaaa aagtgattga aatgaataat ttctacaccg tgactgtgta
     1921 tgaaaaaggg gcggaaatta ttcgtatgat tcacaccctg ttaggcgaag aaaaattcca
     1981 gcaaggtatg caactttatg tggcaagaca cgatggttct gcggcaactt gtgaagattt
     2041 cgttaaagca atggaagatg cttcaggcgt taatcttgaa caattccgcc gttggtatag
     2101 ccaatccggc acgcctgagc tcaccatttc cgatgaatat gatgaaaccc gcaaggcata
     2161 ccgcttgcat atttcacaaa tgacaccggc aacccacgat cagatggata aactcaatct
     2221 gcatattccg cttaaagtag cgttatatgg tgaacaggac ggtaaacgca ttccgttaca
     2281 atatgaatta ttgaccgtaa gcgatgtgtt agatgtaacc gcagaacacc aaacctttga
     2341 gtttcataat gtaacacaac gcccggtgcc tgcattactg tgtgatttct ctgcaccggt
     2401 acggttagat tacaactata ccaccgagca gcttttaacc ttactcaaac acgctgaaaa
     2461 tgattttgta cgctgggatg cagctcaaat gctctatacc aatgaactgc gtgaaaattt
     2521 aagccgttat cagcaacatc aaccactcag cttctcagaa gggttggtga atgccctaac
     2581 gttcgtgttg gataactcag caagcctgcc ggaactaact gcattaaccc tcacattgcc
     2641 aaaagaaacc gagtttgcag aatcgttcaa aaccattgac ccacaaggta ttgcggttgt
     2701 gcgtgaattt atgcagcatt ccattgctca cgccttgcaa gataagttat tagcggtata
     2761 tcaccaaaac caatgcgggg aatatcaagt cgtggctgaa gatattgcta aacgagcctt
     2821 gcgtaatgct tgcttaagct atttagcctt tactgattta ggcaacgcgt tggtgcataa
     2881 gcattacaat aatgccgata atatgactga tacgcttgct gcattacaag ccgcaaccaa
     2941 agcccagcta agttgccgtg atgcattatt agccgatttt gagcaaaaat ggaaacatga
     3001 tggtttagtg atggataaat ggtttatgct acaagccacc cgaccggacg acgatgtgtt
     3061 atccatcgtg caaaatttaa tggaacaccc aagttttaac tttaataacc cgaaccgttt
     3121 acgttcatta gtaggtgcat tctgcggtca aaacccgaaa gccttccacg ccattgacgg
     3181 ttcaggctat cgtttcttgg tagatacgct gattaaactt aacgaaagta acccacaagt
     3241 tgctgctcga cttattgagc cgttaattaa actctcacgc tacgataacc aacgccaaac
     3301 cttgatgcgt cgtggtctag aacgcctgcg tgagctggat aatttagcac gagatctgtt
     3361 tgagaaaatt gaaaaagcgt tagattaaac caagcaatac aagcggtgtc gtttgcaaat
     3421 ttttttacaa atgacaccgc ttgttagtta ataccttacc tcaaaccttt taaacctttc
     3481 tgagccaaga taatcgctca caattacccg ttccaccttt gcagcaggtg agccttgttg
     3541 taaccaattt tccagttgag cgattgtctg ttcatccgct tcagctacca cttctaccga
     3601 gccgtcatca cgatttctga catagccttt tatgcctaat cgattcgctt cctgcaaggt
     3661 aaaaaaccga aaagcgacac cttgaacccg tccgtaaacc gagaataatt ttgcgaccat
     3721 tcttccccca taaaaatagg ctgcttattt ttcaataaac agcccataat gtcttatttc
     3781 acgatgtaga ggttacgcag taaaatatca tcttgcggat ctttaccctc atagcctttc
     3841 acgtaaggtt tcaccaaacg tgggtttaca tagttataaa tcggaataat cgcataatct
     3901 ttggctaact gctcctctgc ttttgcataa gcagctgcac gaccagctgc gtctgtcgct
     3961 ttgtaagatt ctgctaccgc atcatcataa gcttggctct tataaaacgc agtattgttg
     4021 ctggagttcg ataagaaata gttcacaaag gtggttgctt ggttatagtc tgccgaccaa
     4081 cctgcacgag aaacatcata atttgcattg cggcgggtat ctaagaatgt tttccactct
     4141 tggttttcta atttcacatc aacaatgccg tctgtgcctt gtttccataa tgatgaaact
     4201 gcaatcgcaa ttttcttgtg gttgtcgttg gtattataaa gaatagtaaa ttttaacggg
     4261 ttcgctttac tgtaaccggc ttcttccaat aatttcaacg ctttggcttt tctatctgcc
     4321 attgaatctt tggaataagc aggttgctgg attttttcac cctcgtgaat ataaggcggt
     4381 gtaaatacat aagttggtgt ttgaccttgt cctagcactt tatcggtaat aatggtgcga
     4441 tctaaagcta agtttaacgc ctcacgcact ttcacattat caaacggtgc tcgtttatgg
     4501 ttaatttcat agtaataagt agaaagggtt cttgccgtaa acacttcgtt tggaatctct
     4561 tttttcaagg ttgcaaactg ctccggtgga agagcatagt tggtcatatc caaatcaccg
     4621 gcacggtaac gtgaaacatc agttgcggca ttagcaatgg ctaagaaagt cgctttatta
     4681 atcacggttt ctttatcatt ccaataattt ttattacgct caaacacgat tttttcatta
     4741 ataacgtgat cggctaaggt ataagccccg tttccaacca gattgccttt ttgcacccac
     4801 gcatcgccgt gtttctcaac ggttgctttg tgtacaggta ataaagaaga gtgtgttgcc
     4861 agctcaactg catacgggac aggttggctt aactgcacct ggaaagtatg atcatcaatc
     4921 gctttaactg ctagttcctc cggttttttc ttgccgtcaa taatatcttg agcattttct
     4981 acattgagat aagttaaata gcttgaataa ggtgaagccg taagtggatt aaccaaacgc
     5041 tgccaagcaa atacgaaatc gtgagcagtt acggcatcac cgtttgacca tttagcgtct
     5101 tggcgtaatt tgaacgtcca cgttttgtaa tccggtgtat tttcccaact ttctgccaca
     5161 cccggctgaa tattgccgtc agaatctttc gttaccaatc cttcaaataa ctgataagca
     5221 acttgcgatt ccggcacgcc ctcaatttta tgtggatcaa aacttgatgg ctcaaaacca
     5281 ttattaatcg taatttcttg tttttctgca agcgttgttc cttcaggcac tttggctgca
     5341 aacacatttg ccgataagcc taacactaat gaggttgtta ataaggaaag cgagaatttt
     5401 gactgcatat ttgatctcca ttgatgaata ttggcttgca ac
//
LOCUS       DANLYI010000160         5394 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.161, whole genome shotgun sequence.
ACCESSION   DANLYI010000160 DANLYI010000000
VERSION     DANLYI010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 5394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5394
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.161"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            63..1196
                     /gene="carA"
                     /locus_tag="PXD81_002000"
     CDS             63..1196
                     /gene="carA"
                     /locus_tag="PXD81_002000"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HDL5537994.1"
                     /translation="MFKPAILVLTDGSVFSGKSIGADGHTIGEVVFNTAMTGYQEILT
                     DPSYTKQIVTLTYPHIGNTGINLEDKESPNVCASGLIIRDLPLLHSNFRASSSLSDYL
                     IQQNIVAIADIDTRRLTRILREKGAQAGCILVGDATEENIANALAFANSFGSMAGKDL
                     AQSVTCNELYKWTDGEWQLGKGYVPQQQTEFHVVAYDYGVKHNILRMLAERGCRLTVV
                     PAKTSAEAVLALNPDGIFLSNGPGDPEPCDYAITAIRKLLETQKPIFGICLGHQLLGL
                     AAGGKTKKMVFGHHGANHPVQDLDSQKVLITSQNHGFEVDESSLPSNVRVTHRSLFDG
                     TVQGIELTDRPAFSFQGHPEASPGPNDVAYLFDRFVSEMKKVK"
     gene            1299..4505
                     /gene="carB"
                     /locus_tag="PXD81_002001"
     CDS             1299..4505
                     /gene="carB"
                     /locus_tag="PXD81_002001"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HDL5537995.1"
                     /translation="MPKRTDINTILIIGAGPIVIGQACEFDYSGAQACKALREEGYKV
                     VLVNSNPATIMTDPNMADVTYIEPIEWKTVEKIIEKERPDAILPTMGGQTALNCALDL
                     SKNGVLKKYNVELIGATEDAIDKAEDRGRFKEAMTKIGLNTPKSFVCHSFEEALAAQE
                     QVGFPTLIRPSFTMGGSGGGIAYNRDEFIAICERGFEASPTHELLIEQSVLGWKEYEM
                     EVVRDRADNCIIVCSIENFDPMGVHTGDSITVAPAQTLTDKEYQIMRNASLAVLREIG
                     VDTGGSNVQFAINPANGEMIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLNE
                     LRNDITGGLIPASFEPTLDYVVTKIPRFAFEKFANADDRLTTQMKSVGEVMAMGRTFQ
                     ESLQKALRGLETGICGFNLLSEEPEKIRRELANPGPNRILYVADAFGAGFSLEEVHHY
                     SKIDPWFLVQIQDLVLEELALEKKQFSDLDANEIRRLKRKGFSDKRIAQLTKVSEKMV
                     RDYRHTYAILPVYKRVDTCAAEFASDTAYLYSTYEEECEAKPSDRKKVMILGGGPNRI
                     GQGIEFDYCCVHASLALREAGYETIMVNCNPETVSTDFDTSDRLYFEPLTLEDVLEIV
                     RVENPWGVIVHYGGQTPLKLANALSDAGVNIIGTSADSIDAAEDRERFQQILNTLGLK
                     QPANRTARNALEAVALANEVGYPLVVRPSYVLGGRAMQIVYNDEELNRYMREAVSVSN
                     DSPILLDHFLNNAIEVDVDCICDGENVLIGGIMQHVEQAGIHSGDSACSLPAYSLSGH
                     VLDEIRKQTNAMAFALGVKGLMNVQFAVQNEVVYVLEVNPRASRTVPFVSKATGRPLA
                     KIAARVMAGETLADLNALEEIIPSKFFVKEAVFPFIKFPGVDTILGPEMRSTGEVMGI
                     GNTFAEAFFKAQLGAGERIPKVGKVFMSVIEEHKPALIYIAKRFQEQGYGLCATFGTA
                     NFLRQHGIAVQTVNKVREGRPHIVDAIKNGEIALVINTVSGEPEVIADSHSIRRTALQ
                     QRVPIYTTLAGADAISYGVKHINEFDVYSVQELEQKAN"
     gene            complement(4580..>5394)
                     /locus_tag="PXD81_002002"
     CDS             complement(4580..>5394)
                     /locus_tag="PXD81_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HDL5537996.1"
                     /translation="FAKSDRLYNVEDSLQENGSARESLAYSARIEIGLKTFLDNGGFK
                     AFTDNFQNLNGIKQLPGLAVQRLMEQGYGFGGEGGWKTAALVREVKVMGYGLPNGSSF
                     MEDYTYDLDEQNQVVLGAHMLEVCPSIAKEKSTLAIRPLGIGGKADPVRLIFSSKAGK
                     AVNATVVDMGDRFRMVVADLDAIASPNPMPNLPVGHAFWKLQPNFYVGTQAWILAGGA
                     HHSVFSLDIDADMLRMFAEYFGIEFIHINQNTELPQLKNELRWNDLAYKFTK"
BASE COUNT         1537 a         1241 c         1214 g         1402 t
ORIGIN      
        1 ttaaacttag tcgcctactt ctaaggctac acagataaaa tttgatgatt aggagtaaaa
       61 caatgttcaa accagcaatt ctggttctca ctgacggttc tgtcttttcc ggcaaatcca
      121 ttggtgctga cggtcacact atcggtgaag tggtatttaa cactgcgatg accggttatc
      181 aagaaatcct taccgatcct tcttatacta aacaaatagt caccctgact tacccccaca
      241 ttggcaatac cggcattaat ttagaagata aagaatcccc caatgtttgt gcctccggct
      301 taattattcg tgatcttcct cttctccata gcaacttccg tgcgtcctcc tcactttcag
      361 attatctcat tcaacaaaac attgtcgcta ttgccgatat tgatacccgc cgtttaaccc
      421 gaattttacg cgaaaaaggg gcacaagccg gttgtatttt agtaggcgat gccacagaag
      481 agaatattgc taacgctttg gcatttgcca acagtttcgg ttctatggct gggaaagatt
      541 tggctcaaag tgtcacctgc aatgaattat ataaatggac agatggtgaa tggcaattag
      601 gtaaaggtta tgtaccgcaa caacaaaccg agttccacgt agttgcctat gattacggtg
      661 taaaacacaa cattttgcga atgcttgccg aacgaggctg tcgcttaacc gttgtgccgg
      721 caaaaacctc tgctgaagct gtgcttgcgt taaatccgga cggtattttc ctttctaacg
      781 gcccggggga cccggagccg tgcgattatg ccattaccgc aatccgaaaa ttattggaaa
      841 cgcaaaaacc cattttcggc atttgcttag ggcatcaact tttaggttta gcggcaggcg
      901 gtaaaaccaa aaaaatggtg tttggtcatc acggagctaa tcacccggta caagatttag
      961 acagccaaaa agtgctcatc accagccaaa accacggttt tgaagtagat gaatccagct
     1021 taccatctaa tgtgcgagtg actcaccgct cactgtttga cggcacggtg caggggattg
     1081 aactcactga tagaccggcg ttttcgttcc aaggtcaccc ggaagccagc ccggggccga
     1141 atgatgttgc ctatttattt gaccgatttg tcagtgagat gaaaaaagtg aagtaaggac
     1201 gtacctatgt gtgtccatca agcggtcgaa atttgcaaaa aacttaccaa aaatgaccgc
     1261 ttgtagcctg aaataaacga gataaaagag agcccaaaat gccaaaaaga acagacataa
     1321 acactatcct aattatcggt gccggtccga ttgtgatcgg tcaagcgtgt gaatttgact
     1381 actccggagc ccaagcgtgc aaagcgttgc gtgaagaagg gtataaagtg gtgttggtga
     1441 actccaaccc tgcaaccatt atgaccgacc cgaatatggc agatgtgact tacattgagc
     1501 cgattgaatg gaaaacggtt gaaaaaatca ttgaaaaaga acgccctgat gccattttac
     1561 cgactatggg cgggcagaca gcgttgaact gtgccttaga tctttcgaaa aacggtgtgc
     1621 tgaaaaaata taatgtagag ctgatcggag ctaccgaaga cgcgattgat aaagccgaag
     1681 atcgtggtcg ttttaaagaa gcaatgacta aaatcggctt gaatacgcca aaatcattcg
     1741 tttgccacag ttttgaagag gccttagcag cacaagagca ggttggcttt ccaaccctga
     1801 ttcgcccctc tttcacgatg ggaggctccg gcggtggtat tgcttataat cgagatgaat
     1861 ttatcgccat ctgcgaacgt ggttttgaag cctcgcctac tcacgaatta ctgattgaac
     1921 aatccgtact aggctggaaa gagtatgaaa tggaagttgt gcgtgaccgt gccgataact
     1981 gcattatcgt ctgctcgatt gaaaacttcg acccgatggg tgtacacacc ggcgactcca
     2041 ttaccgttgc tccggcacaa acgctaaccg ataaagagta tcaaattatg cgtaacgctt
     2101 cgctggcggt attgcgtgag atcggcgtgg acaccggcgg ttcaaatgtg caatttgcga
     2161 ttaaccctgc aaacggcgag atgatcgtca ttgaaatgaa ccctcgtgtg agccgttctt
     2221 ctgcactcgc atctaaagcc accggcttcc cgattgccaa agttgcggcg aaattggctg
     2281 taggctacac actcaacgaa ttgcgtaacg acatcaccgg agggctgatt ccggcttcat
     2341 tcgagccgac tttagattat gttgtgacca aaattccacg cttcgccttt gaaaaatttg
     2401 ccaatgcaga cgaccgatta accactcaaa tgaaatcggt aggcgaagta atggcaatgg
     2461 gaagaacctt ccaagagagt ttacaaaaag ccttgcgagg cttggaaacc ggcatttgcg
     2521 gctttaactt gctttccgaa gagcctgaaa aaatccgccg tgaacttgcc aaccccggac
     2581 caaaccgtat tttgtatgtt gccgatgcct ttggagcagg cttttcattg gaagaagttc
     2641 accactattc taaaatcgac ccgtggttct tggtgcaaat tcaagactta gtacttgaag
     2701 aattagcgtt agagaaaaaa cagtttagcg atctagatgc taacgaaatc cgccgcttaa
     2761 aacgcaaagg cttctcagac aaacgtatcg cacagcttac caaagtcagc gaaaaaatgg
     2821 tgcgggatta ccgccatact tacgctattc taccggtgta taaacgggta gatacctgtg
     2881 cagccgagtt tgcctcagat acggcttacc tctattctac ttacgaggaa gagtgtgaag
     2941 ccaaaccgtc tgatcgcaaa aaagtgatga ttttaggcgg cggaccaaac cgtatcgggc
     3001 aggggattga atttgactac tgctgcgttc acgcctcgct tgctctgcgt gaggcaggtt
     3061 atgaaaccat tatggtgaac tgcaaccctg aaaccgtttc caccgatttc gacacctccg
     3121 accgcctcta tttcgagccg ctaacattag aagatgtact ggaaattgtg cgtgtagaaa
     3181 acccgtgggg cgtgattgtg cattacggcg gtcaaacacc actgaaatta gcgaatgcct
     3241 tatctgatgc cggtgtaaac attatcggga cttccgccga cagcattgat gccgccgaag
     3301 accgtgaacg tttccaacaa attctaaaca ctctcggttt aaaacagccg gcaaatcgca
     3361 ccgcccgtaa tgcgttggaa gcggtggcat tggcaaatga agtaggctat ccgctggtgg
     3421 ttcgcccatc ttatgtgcta ggtggtcggg caatgcaaat tgtgtataac gatgaagagc
     3481 taaaccgcta tatgcgtgaa gcggtatcgg tttccaacga cagcccgatt ttactcgacc
     3541 acttcctcaa taatgcgatt gaagttgatg tggattgcat ttgtgacggc gaaaatgtac
     3601 tcattggcgg cattatgcaa cacgttgagc aagccggtat tcacagtggc gatagtgcct
     3661 gctcactacc tgcctattcg ttaagcggtc atgttttgga cgaaattcgc aaacaaacca
     3721 acgcaatggc gtttgctctc ggtgtgaaag gcttgatgaa cgtacaattt gcagtacaaa
     3781 atgaggtcgt ttatgtgttg gaagttaacc ctcgagccag ccgtactgta ccgtttgtca
     3841 gcaaagctac cggtcgccca ctggcaaaaa tcgctgctcg cgtgatggct ggtgaaaccc
     3901 ttgccgactt aaatgcatta gaggaaatta tcccaagcaa attcttcgtt aaagaagcgg
     3961 tattcccatt catcaaattc ccgggtgtgg ataccattct cgggcctgaa atgcgttcta
     4021 ccggcgaagt gatgggcata ggtaatacct ttgctgaagc gttcttcaaa gcacaactgg
     4081 gtgccggtga acgaattcca aaagtcggca aagtgtttat gtcggttatt gaagagcata
     4141 aacctgcttt aatttacatt gccaaacgtt tccaagaaca aggctacggt ttatgtgcca
     4201 ccttcggcac tgcaaacttc ctacggcaac acggaattgc agtccaaacg gtaaacaaag
     4261 ttcgtgaagg gcgaccgcat attgtcgatg cgatcaaaaa cggcgaaatt gcgttggtaa
     4321 tcaacaccgt cagcggcgag cctgaagtga ttgccgacag ccactctatc cgccgtaccg
     4381 ccttgcaaca acgtgtgcct atttacacca cccttgcagg tgctgatgct atctcctacg
     4441 gcgtaaaaca tatcaatgag tttgatgtgt attcagtgca ggaattagaa caaaaggcta
     4501 actaaaataa agctaaaatg acaagcggtc agattttgca aattttttac aaattctgac
     4561 cgcttgtgtt tgtagaggat tatttagtaa atttatatgc taaatcattc cagcgtaatt
     4621 catttttgag ttgtggtagc tcggtatttt ggttgatatg gataaactca ataccaaaat
     4681 attcagcgaa cattcgtagc atatcggcat caatatctaa actaaataca ctatgatgag
     4741 caccgccggc tagaatccat gcttgagtac ctacataaaa atttggttga agcttccaaa
     4801 aagcatgccc aactggtaaa tttggcattg gatttggtga ggcgatagcg tctaaatccg
     4861 caacaaccat tctaaaacgg tctcccatat ctacaacggt tgcattcact gcttttcccg
     4921 ccttactact gaaaattaag cgaacaggat ctgccttacc gccaatacct agcggtcgaa
     4981 tggctaatgt tgatttctct ttggcaatag acggacaaac ttcaagcata tgagccccca
     5041 ataccacttg gttttgttca tctaaatcat aggtgtagtc ttccataaag gaagaaccgt
     5101 ttggtaagcc ataacccatc actttcactt cacgcacaag agctgcggtt ttccaacctc
     5161 cttcaccacc aaatccgtag ccttgttcca ttaaacgctg aacagccaag cccggtaatt
     5221 gtttaatccc atttagattt tggaaattat cagtaaaagc tttaaatcca ccattatcta
     5281 agaatgtttt taagccgatt tcaatacgag cagaatatgc gagagattct cttgctgaac
     5341 cattttcttg caagctatcc tcaacattat acaagcggtc agattttgca aaaa
//
LOCUS       DANLYI010000161         5364 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.162, whole genome shotgun sequence.
ACCESSION   DANLYI010000161 DANLYI010000000
VERSION     DANLYI010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 5364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5364
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.162"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            complement(40..1323)
                     /locus_tag="PXD81_002003"
     CDS             complement(40..1323)
                     /locus_tag="PXD81_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL5537997.1"
                     /translation="MTACHEPKRSIWQQIFTKNMLLCVYTGFCSGLPLFVLIQLMPAW
                     LTSANLDIKTIAAFTLTSLPYTWKFLWAALLDRYFPPFLGRRKSWIFISQIGLLVILA
                     SFGLFNPVQDISIIVTLSVLLAFLSATQDIVVDAFRREILSDNELGLGNSIHVNAYRI
                     AGLIPGGLSLFLADHYAWDTVFLITAAFLVPCLFVTLIIAAEPNHKPIDRSKPFYMAF
                     VDPFKEFFTRKGVAGAIGLILFIFLYKLGDSLATTLQTKFILDMGFTKSHIAGIVKIT
                     SLWCSIGGGIIGGVAMLKLGVNRALWLFGFVQLITILGFAYLASFEHFPTESIGAAEL
                     WKLGLVMAGEYLGVGLGTAAFVAFMARETNPAYTAMQLAIFTSLAALPSKMLGAYTGH
                     LVEMMGYYQFFWLCFFIGIPGMLMLFKVAPWGQAE"
     gene            1489..5205
                     /gene="metH"
                     /locus_tag="PXD81_002004"
     CDS             1489..5205
                     /gene="metH"
                     /locus_tag="PXD81_002004"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HDL5537998.1"
                     /translation="MNTQHRTELLQNALTERILILDGAMGTMIQKYKLTESDFRGERF
                     KNSTIDLKGNNDLLTLTQPLLISAIHEKYLAAGADIIETNTFSATTIAQADYELESIA
                     YELNLVGAKLARLAADKFSIPEKPRFVAGILGPTNRTCSISPDVNDPGFRNITFMQLV
                     EAYAEATRGLIEGGADLIMIETIFDTLNAKAAVFAIETVFDELGVKLPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAKPLTFGLNCALGPKELRPYVEMMSKISETFVSVHPNAG
                     LPNAFGGYDLGAEEMATYIKEWAELGWLNIVGGCCGTTPEHIQAFAEATKGVAPRKLP
                     EIPTAMRLSGLEPLTIDDNSLFVNVGERNNVTGSAKFKKLIKEEKFAEAIEIAISQVE
                     NGAQVIDVNMDEALLDSKKCMTRFLNILATEPEAAKVPIMIDSSKWEVIEAGLQTVQG
                     KPIVNSISLKEGEEKFIHQAKLCRRYGAAVVVMAFDEVGQADTEERKVEICTRAYRIL
                     VDQLGFPPEDIIFDPNIFAIATGIEEHNNYGVDFIQACERIKRDLPHAKISGGVSNVS
                     FSFRGNNVVREAIHAVFLYYAIKAGMDMGIVNAGQLAIYDDLDPELREAIEDAVLNHR
                     HDTTDRLLEISEKYRGVKVESADESAAEWRNLPVAERLKHALVKGITTYIIEDTEEAR
                     QQFASPLEVIEGPLMAGMDVVGDLFGDGKMFLPQVVKSARVMKQSVAYLEPYINATKQ
                     KGSSNGKMVIATVKGDVHDIGKNIVSVVLQCNNFEVIDLGVMVPADVIIETAIREKAD
                     LIGLSGLITPSLDEMEYFLGEMNRLKLDIPVLIGGATTSKEHTAIKLYPKYNYPVVYT
                     ANASRCVTVCATLMNPEAKAEFWEKTKAEYEEIQRKFAVRKPLRNGLSIEEARANRFD
                     GFSGEWADYVPPTPKQTGIIEYKNVPIATLRKFIDWSPFFMLWGLMGGYPDAFDYPEG
                     GEEARRVYNDAQRVLDEFEQNGKLNPSGIMGIFPAVREGDDIAILNEDRTACIGKSYH
                     LRQQSERGKNSKSPYNLCLSDFIADRESGQQDWFGMFAVCAGIEEHYLVESYKAAGDD
                     YNAILLQAVGDRLAEAMAEYLHFELRTKVWGYTVEDEDNGRLIREEYIGIRPAPGYPS
                     CPEHTEKQIIWDLLEVEQRIGMKLTESYAMWPAASVCGWYFTHPTSNYFTLGKIDEDQ
                     VLDYAKRKGWDELTAKKWLSVAMKQDVKSQAENI"
BASE COUNT         1579 a         1232 c         1301 g         1252 t
ORIGIN      
        1 ggtcggatat ttaataaaaa atcttatttt tatgaatagc tattcagctt gtccccaagg
       61 agcaacctta aacaacatta gcattcccgg aataccaata aagaaacaga gccagaagaa
      121 ttggtaatag cccatcattt ccactaaatg ccctgtgtat gcccctaaca ttttgcttgg
      181 tagggcagca aggctggtga aaatggcgag ctgcattgca gtgtaagcgg gattagtttc
      241 acgagccata aaggcaacga aagccgctgt gcctaaaccc acgcccagat attcacctgc
      301 catcactaag cctaatttcc aaagctcggc agctccaatg ctttcggtag ggaaatgctc
      361 aaaactggca aggtaagcga agcctaaaat agtaattaat tgtacaaatc cgaacagcca
      421 taaggctcgg ttaacgccca atttcaacat tgccacgcca ccaataatac cgccgccaat
      481 gctgcaccaa agtgaagtta ttttcacaat gccggcaatg tgcgatttag taaaacccat
      541 atccaaaata aatttggttt gtagcgtagt tgcaagggaa tcgcctaatt tatataggaa
      601 aataaataga atcagcccaa tcgctcctgc cacgccttta cgggtgaaaa actctttgaa
      661 cggatcgaca aacgccatat aaaacggctt agaacggtcg attggcttat gattcggctc
      721 ggcagcgatt attagcgtaa cgaataagca aggcactaaa aaagcagcgg taatcagaaa
      781 tacggtatcc cacgcatagt gatcggctaa aaataatgaa agcccacccg gaatcagccc
      841 tgcaatacga taagcattaa cgtggataga gttacctaac ccgagttcgt tatccgataa
      901 aatttcacgc cgaaaggcat ccaccacaat gtcttgcgtg gcggaaagaa aagctaatag
      961 cacagaaaga gtaacaataa ttgaaatatc ttgcactggg ttgaacaagc caaagctggc
     1021 taaaatcacc aatagcccga tttgtgaaat gaaaatccag cttttgcgtc tgcctaaaaa
     1081 aggtggaaag tagcgatcta acaatgcagc ccacaggaat ttccacgtgt agggcaaaga
     1141 ggtcagggtg aaggcggcaa tagttttaat atctaaattg gcactggtta gccaagccgg
     1201 cattagctga attaacacaa acaacggtaa accggaacaa aagccggtgt aaacacaaag
     1261 caacatattt ttagtaaaaa tctgttgcca gatagagcgt ttgggttcgt gacaagcggt
     1321 catatttttc ctacattttg caaaataaaa aggggaagaa ctgagccaat atagcataat
     1381 tttaaaggcg actctaaccc tttccatttc gtcacagata aggtttgaca gccttgcgac
     1441 atatttctat tattcctttt aatcaaacta aaaaactgga agcagagtat gaacacgcag
     1501 catagaaccg aattacttca aaatgccctt accgaacgca ttcttatctt agacggtgcg
     1561 atgggaacga tgatccaaaa atataaacta accgaatctg atttccgagg tgaacgtttt
     1621 aaaaacagta ccatcgatct taaaggcaat aacgacctac tcacactcac ccagccactc
     1681 ctgatttctg ctattcacga gaaatattta gccgccggtg cggatattat cgaaactaat
     1741 acctttagtg cgacgaccat tgctcaagca gattatgaac ttgaaagtat tgcttatgag
     1801 ctgaatcttg tgggggcaaa acttgcccgt cttgccgccg ataaattctc catccctgaa
     1861 aaacctcgtt ttgttgccgg tattcttggg ccgactaacc gtacttgttc gatttcccct
     1921 gatgttaatg accccggttt ccgtaacatt acctttatgc aactggtgga agcctatgcc
     1981 gaggcaaccc gtggtttgat tgaaggtggg gcggatctga ttatgatcga aaccattttc
     2041 gataccctga atgccaaagc ggcggtgttt gcaattgaaa cggtgtttga tgaactgggc
     2101 gtgaaattgc cgataatgat ctccggcacc attaccgatg cctcagggcg aacgctgtca
     2161 gggcagacca cggaggcgtt ttataactcg ctccgccacg ccaaaccgct gacgttcggc
     2221 ttgaactgcg cgctcgggcc gaaggaactc cgcccttatg tagagatgat gtcgaaaatc
     2281 agtgagactt ttgtttcggt tcacccgaat gccggcttgc caaacgcttt tggcgggtac
     2341 gatttagggg cggaagagat ggcgacttac atcaaagagt gggcagaact cggctggctg
     2401 aatattgtcg gcggctgttg tggcaccacg ccggagcata tccaagcctt tgccgaagca
     2461 accaaaggcg ttgcgcctcg taaattacct gaaattccaa ccgctatgcg tttgtcgggc
     2521 ttagaaccgc tgacgattga cgataattcg ctgtttgtga atgtgggcga acgcaataac
     2581 gtcaccggtt cggcaaaatt taagaagctg attaaagaag aaaaatttgc ggaagcgatt
     2641 gaaattgcca tcagccaagt ggaaaacggg gcgcaggtga ttgacgtgaa tatggacgag
     2701 gcgttgctcg attccaaaaa atgtatgacc cgtttcctca acattcttgc taccgaacct
     2761 gaggcggcaa aagtgccgat tatgattgac tcctcgaaat gggaggtgat cgaggcgggc
     2821 ttgcaaacgg tgcagggcaa gccgatagtg aactccattt cactcaaaga gggcgaagag
     2881 aaatttattc accaagccaa actctgccgc cgctatgggg ctgcggtggt ggtgatggca
     2941 tttgacgaag tcgggcaagc ggataccgaa gaacgcaagg tagaaatttg tacccgagct
     3001 tatcggattt tggttgatca gctcggcttc ccaccggaag acattatttt cgacccgaac
     3061 atttttgcga ttgccaccgg tattgaagaa cacaataact acggcgtgga ttttatccaa
     3121 gcctgtgagc ggattaaacg agacttgcca cacgccaaaa tttccggcgg ggtgtcgaat
     3181 gtctctttct ctttccgagg caacaatgtg gtgcgtgagg ctattcacgc cgtgttcctc
     3241 tactatgcga tcaaagccgg aatggatatg gggatcgtga acgcagggca gttggcgatt
     3301 tatgacgacc tcgaccctga actgcgagag gcgattgaag atgcggtgct gaaccaccgt
     3361 catgacacta ccgaccgcct gttggaaatt tccgaaaaat atcggggcgt aaaagtagaa
     3421 agtgccgatg aaagtgccgc cgagtggcga aatttacctg tggcagagcg tttaaaacac
     3481 gccctcgtaa aaggcataac gacctacatt attgaagata ccgaagaggc tcgccagcaa
     3541 tttgcctctc cgctggaagt gattgaaggg ccgctcatgg cgggtatgga cgtggtgggc
     3601 gacctgttcg gagacggcaa aatgttcctg ccgcaagtgg tgaaatcggc tcgggtaatg
     3661 aaacagtccg ttgcctattt agagccgtac atcaacgcca ccaagcagaa aggatcgagc
     3721 aacggcaaaa tggtgatcgc caccgtgaaa ggtgatgtac acgacatcgg caaaaacatc
     3781 gttagcgtgg tgttgcagtg caataacttt gaagtgatag acttaggcgt gatggtgcct
     3841 gccgatgtaa ttatcgaaac tgctattcgg gagaaagccg atctcattgg gttaagcgga
     3901 ctaattaccc catcgctgga cgaaatggag tattttttag gtgaaatgaa ccgcttgaag
     3961 ctcgatattc cggtgctgat tggcggcgca acgacctcaa aagagcatac ggcgatcaaa
     4021 ctttatccga aatataacta ccccgtagtc tataccgcca acgcttcacg ttgcgtaacc
     4081 gtctgtgcga ccttgatgaa ccctgaggca aaagccgaat tttgggaaaa aaccaaagcc
     4141 gaatacgaag aaattcagcg taaatttgcc gttcgcaaac cacttcgcaa cggtttatct
     4201 attgaggagg ctcgagccaa tcgttttgac ggatttagtg gcgaatgggc agactatgtg
     4261 ccgccaacgc caaaacagac cggcattatc gagtacaaaa atgtaccgat tgccacccta
     4321 cgcaaattta tcgactggtc gccgttcttt atgctctggg gcttaatggg cggttatcct
     4381 gatgcctttg attatccgga aggcggcgaa gaggcacgcc gtgtctataa cgatgcccaa
     4441 cgggtgctgg acgaatttga gcaaaacggc aagctcaacc caagtgggat tatgggcatt
     4501 ttccctgccg tgcgtgaggg cgatgatatt gcaattttaa acgaagatcg caccgcttgt
     4561 atcggcaaat cctaccacct ccgccaacaa tccgaacgag gcaaaaacag caaaagcccg
     4621 tacaacctct gtttaagcga ttttatcgcc gaccgagaaa gcggacaaca agactggttc
     4681 ggaatgttcg ccgtttgtgc cggcattgaa gaacactatt tagttgaaag ctacaaagcg
     4741 gcaggcgatg actacaacgc cattttgctc caagccgttg gcgaccgctt ggcagaagca
     4801 atggcggaat acctacactt tgagctacgc accaaagtct ggggctacac cgtggaagac
     4861 gaagacaacg gacgcttaat ccgtgaagaa tacatcggca tccgccctgc gcccggctac
     4921 ccaagctgcc cggaacacac cgagaaacag ataatttggg acttgttaga ggtggaacaa
     4981 cgcatcggca tgaaactcac cgaaagctat gccatgtggc cggcagcaag tgtctgcggc
     5041 tggtacttca cccaccctac cagcaactac ttcactctcg gcaagattga tgaagatcag
     5101 gtgttggatt acgccaaacg caaaggctgg gacgaactca cggcgaagaa atggttaagt
     5161 gtggcgatga aacaagatgt caagtcgcag gctgaaaaca tttaatgtag tggcgggttt
     5221 taacccgcct gttacaagcg gttggttttg agataaaata tgcaaaattt atctctcttt
     5281 tatgcaaaga agttatcatc gagaacaaaa caatgattga aaaacaaaat tatcacttag
     5341 tacaactttt aaaagacagt aatt
//
LOCUS       DANLYI010000162         5355 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.163, whole genome shotgun sequence.
ACCESSION   DANLYI010000162 DANLYI010000000
VERSION     DANLYI010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 5355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5355
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.163"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            complement(295..888)
                     /locus_tag="PXD81_002005"
     CDS             complement(295..888)
                     /locus_tag="PXD81_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5537999.1"
                     /translation="MHNNLAIFYCGTNGAGKSTLRSFNQDKVEIVIDSDNIAMQINPL
                     NPRLADIEAGRKAIELFQFAVKNQVAFSMESTLSGKSILKRMENAKAGYFKTRLNYIG
                     VDSYKINIARVKARVKAGGHFIDEETIRKRYHISRENLIEAIFINDETLIYDNSETKP
                     NLILQIADKKVIKISDKIPNWCNQLCDELVELGFKTT"
     gene            complement(878..1048)
                     /locus_tag="PXD81_002006"
     CDS             complement(878..1048)
                     /locus_tag="PXD81_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5538000.1"
                     /translation="MTSREELLKKQRELDILFTAWFEEKKKHEVLTYRRENGDLIQHY
                     PDGTEKVIEYAQ"
     gene            complement(1050..1792)
                     /locus_tag="PXD81_002007"
                     /pseudo
     CDS             complement(1050..1792)
                     /locus_tag="PXD81_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250915.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
     gene            complement(1789..2232)
                     /locus_tag="PXD81_002008"
     CDS             complement(1789..2232)
                     /locus_tag="PXD81_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL5538001.1"
                     /translation="MLKNLSWRAKRFDELSTQELFEIYHARTAVFVVEQNCPYQEVDN
                     KDLQAVHFFTKNAKNLTAYCRLIPANDGVHIGRVLVVKENRGSGLARELVQKAMDYCG
                     EHFPAQPIHAQAQSYLQGFYESFGFKAVSEVYLEDGIAHLDMVLE"
     gene            complement(2226..2663)
                     /locus_tag="PXD81_002009"
     CDS             complement(2226..2663)
                     /locus_tag="PXD81_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HDL5538002.1"
                     /translation="MKQVQFYLLSQADAAELSAAEMQACELAAQAWRLGKRVLIACET
                     EQQALNIDEALWARDPDSFVPHNLSGEITTYPTPIEISWKGKRNAQRRDLLISLQTQL
                     PEFIGSFTQIIDFVPVEETAKAQARDRYKQYRQLGWQLETIQC"
     gene            2902..3345
                     /gene="lysM"
                     /locus_tag="PXD81_002010"
     CDS             2902..3345
                     /gene="lysM"
                     /locus_tag="PXD81_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005598764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein LysM"
                     /protein_id="HDL5538003.1"
                     /translation="MGLFDFVGDIGRKLFNKEEDASKAVTEHLAEDNPGVENVNVTVE
                     NGVAKISGIASTAAAVEKAVLMAGNVAGITEVNIDEVTLPDDVQLASDDELYVIQKGD
                     TLWEIATKAYGNGAKYKAIVEANKEVIKDENKIFPGQKIRIPKGL"
     gene            complement(3399..4250)
                     /locus_tag="PXD81_002011"
     CDS             complement(3399..4250)
                     /locus_tag="PXD81_002011"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005701564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HDL5538004.1"
                     /translation="MKQYLDLCNRIVSEGKWVANERTGKHCLTVINADLTYDVEKGEF
                     PLVTTRRSYWKAAIAELLGYIRGYSNAADFRQLGTKSWDANANENAQWLANPYRKGED
                     DMGLVYGAVGRNFPKPDGGSVDLLRQIVDDLKKGIDNRGEIYTFYHPGAFHMGCLRPC
                     LHSHHFSLLDGTLYLNSTQRSADVPLGLNWNMIQCYTFLALMAQITGHKAGQAFHKIV
                     NAHIYEDQLELMRDVQLKREPLNAPKLIINPDIKSLEDLETWVSLDDFKVEGYEYHPA
                     IQYPFSV"
     gene            4343..4894
                     /locus_tag="PXD81_002012"
     CDS             4343..4894
                     /locus_tag="PXD81_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5538005.1"
                     /translation="MKGIMKSILVFSTAVFLAACSSNKSGWTRLNDNDVDQKSYAIAY
                     GATAQTYADRVNESYDINSFMNGVNDWYTKKVTLPIEQIRAITINRMLDHNVYAYYSG
                     VIYAADLQANFNHLDPNCWGLVQPASMTQGIHDAMLDLQKNKVRGDEYIKNGVEQILH
                     LCVKTIAEDENESQSKGKTKKHK"
     gene            5103..5197
                     /locus_tag="PXD81_002013"
     tRNA            5103..5197
                     /locus_tag="PXD81_002013"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5138..5140,aa:Ser,seq:gct)
     gene            5211..5287
                     /locus_tag="PXD81_002014"
     tRNA            5211..5287
                     /locus_tag="PXD81_002014"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5245..5247,aa:Arg,seq:acg)
BASE COUNT         1569 a         1025 c         1053 g         1708 t
ORIGIN      
        1 tttttatgag taccaataac ttttttagac tttttcctat tctgtttcat ctttgtgttt
       61 ttgttccaat atccccgatg gtgatcgtct tcgacgagag cttatttatt taagaaccta
      121 tcagagatag gcataagcgg tctgtttcga gaagtttttt gcaaagtttt tctttaatcc
      181 gacagcttgt cacccacaca caagccgtgt ggggttacgc atagctgagc cactttgtgg
      241 ctctaagtta cagctccaaa ggagctggga ttgtcgagcc tagcacggct cgtgctaggt
      301 ggttttaaac cctaattcca ctaattcatc acaaagctga ttacaccaat tcggtatttt
      361 gtctgaaatt tttatcactt tcttatcagc aatctgcaaa atcaaattcg gcttggtttc
      421 tgaattatca taaatcagtg tttcatcatt gataaaaatc gcttcaatta aattttcacg
      481 gctaatgtga tagcgttttc taatggtttc ttcatcaata aaatgtccgc cggctttcac
      541 tcttgctttt acccgtgcga tgttaatttt atagctatcg acaccaatat aatttaatcg
      601 agttttgaaa tatcctgctt tggcattttc cattcgtttt agaatagatt tacccgagag
      661 ggttgattcc attgaaaatg caacctgatt ttttactgca aactgaaata gttcgattgc
      721 ttttctgccc gcctcaatat cggccaatct tggattgagt gggttaatct gcatcgcaat
      781 attatcggaa tcaatcacaa tctcgacttt atcttgattg aaagaacgta aagtagattt
      841 gccggctccg tttgtgccac agtaaaaaat tgctaggtta ttgtgcatat tcaatcactt
      901 tttccgtacc gtccggataa tgctgaatta aatccccatt ttctcggcga tacgtcagca
      961 cttcgtgctt tttcttttct tcaaaccatg ctgtaaagag aatatccagt tcacgttgtt
     1021 ttttcagtaa ttcttcacga cttgtcattt tatgtcctct gtttcagaat cgccaaatac
     1081 ctttccaact cagctaattc atactgacta atcaattcaa ccaaactact aatatcttgt
     1141 tgatttttat ggaaatcatc aaaacattgg taatattggg ctcgattggt aaatttaata
     1201 tcaatcggta aatagccctg ttttaataat tcaaaattaa tcagtaatcg ccctgtgagc
     1261 ccgtttccat caataaaagg gtgaatgctt tcaaaggcta aatgcagcca actgattgcc
     1321 tctatgggat gaagtgcggt cagtttttgt tgatattttt caatcaataa ttgcatttct
     1381 tcggcaatta aataaggggg ggggcatttt ctgcccctaa aattcgtact ggaattttgc
     1441 gataaacccc tcggctttcc gcattgccca ttaataccag agaatgaata tctttaatcg
     1501 ctcgttcgct taatggttct ttattggcga ctaattcata aatatagttg aatgcatcct
     1561 taaaaccgat aatatctaaa tgctcccgaa tcggtttttc ggcaatggtc attccctctt
     1621 ttaaaatgag caccgtttca cgcaaagtaa tggtattgcc ttcaattgca ttggaattat
     1681 agctgctttc aataataaac tcttcccgca gtcgttgaac ttcgctttca gataatggac
     1741 ggcgttgtga aagtgctgtt tttaattgtt cgattttagc taatttattc attctaacac
     1801 catatccaaa tgcgcaatcc catcttccaa atacacttct gaaaccgctt taaacccaaa
     1861 actttcataa aacccttgca ggtaactttg ggcttgggcg tgaatgggtt gagcaggaaa
     1921 atgttcgcca caataatcca tcgctttttg taccaattcc cttgctaagc ctgagccacg
     1981 attttctttc accaccaaca ctctacctat atgcacacca tcattggctg gaattaaacg
     2041 gcagtaagcg gtcagatttt ttgcattttt tgtaaagaaa tgcaccgctt gcaggtcttt
     2101 attatcgact tcttggtaag ggcagttttg ctcaaccaca aacaccgctg tacgtgcgtg
     2161 gtaaatctca aacaactctt gggtggagag ttcatcaaag cgttttgcgc gccaagagag
     2221 gttttttagc attgtatcgt ttccagttgc caaccaagtt ggcggtattg tttatagcgg
     2281 tcacgagcct gtgctttggc ggtttcttcc accggcacga aatcaataat ctgggtaaaa
     2341 ctgccgataa attcggggag ttgggtttgg agtgaaatca acaaatcccg acgttgagca
     2401 ttgcgtttac ctttccacga aatttcgata ggcgttgggt aagtggtgat ttctcccgat
     2461 aaattgtgcg gcacgaacga atccggatct cttgcccaaa gtgcttcgtc aatatttaac
     2521 gcttgttgct cggtttcgca tgcaatcagt acccgtttac ctaatcgcca cgcttgagcc
     2581 gcaagttcgc aggcttgcat ttcagcagcg gaaagctcgg ctgcatcggc ttggcttaat
     2641 agataaaatt gtacttgttt cacagaatgt tcccagaaat tcaatcagat atttaaactt
     2701 aatattctag ctgaaaatga gtaaaatata aaatctaagc gtttgttttt gcaaaaaatt
     2761 gagaaaaaat gaccacttgt gttgcgttgc cttgaggaac taattagctt ttagacgttc
     2821 taagagaaca atgcactatt tcaatagttg gaaattgtgt agaataccca cttcatttag
     2881 ctattttata aggaacttac tatgggctta tttgattttg ttggcgacat tggtcgtaaa
     2941 ttatttaata aagaagaaga tgcttcaaaa gcggttacag agcatttggc ggaagacaat
     3001 ccgggcgttg aaaatgtaaa cgtaacggtt gaaaacgggg tagcgaaaat ttcaggcatt
     3061 gcttcaaccg cagcagctgt tgagaaagcg gtattgatgg cgggtaatgt ggctggtatc
     3121 actgaggtaa atattgatga agtgaccttg cctgatgacg tgcaacttgc aagtgatgat
     3181 gagctttatg ttatccaaaa aggggatacg ttgtgggaaa tcgccacaaa agcttatggt
     3241 aacggtgcaa aatataaagc gattgtagaa gcaaataaag aagttattaa agacgaaaat
     3301 aaaatcttcc cagggcaaaa aattcgtatc ccgaaaggtt tataatcaaa ttgaaagagc
     3361 taagctggtc gaaagtttag ctctttttgt aggtggtgtt agactgaaaa tggatattga
     3421 atggcagggt ggtattcata accttcaact ttgaaatcat ctaagctcac ccaagtttct
     3481 aaatcttcta aggattttat atcaggatta atgattaatt taggtgcatt gagtggttct
     3541 cgcttaagtt gaacatcacg cattaattct agttgatctt cataaatatg cgcattgaca
     3601 attttatgga acgcttgtcc tgccttatgc cctgtaattt gagccattaa agctaaaaag
     3661 gtatagcatt gaatcatatt ccaatttaac ccaagcggta catctgccga acgctgagta
     3721 ctattcaggt aaagcgtgcc atcaagtaga gaaaaatgat ggctgtgtaa acaaggacgt
     3781 aagcagccca tatggaaagc gccggggtgg tagaaggtat aaatttcacc acggttatca
     3841 attccttttt tcaaatcgtc cacaatttgt cgcaataaat cgactgagcc accgtctggt
     3901 ttcgggaaat tacgccctac tgcgccgtaa accaagccca tatcatcttc cccttttcga
     3961 tagggattag caagccattg tgcattttca tttgcattag cgtcccacga ttttgtgcct
     4021 aattgacgga aatcagcagc attactatag cctcgaatat agcctaaaag ttcggcaatc
     4081 gcagctttcc aataactacg gcgagtggta actaaaggaa attctccttt ttctacgtca
     4141 taagttaagt cagcattaat gactgtgagg cagtgtttgc ctgtacgttc atttgcaacc
     4201 catttgcctt cgctaacgat gcggttacat aagtctaaat actgtttcat tatttacctc
     4261 ataaaaaatt ttaggtaatt ttgccattta ttcggtaaaa tagctaaaac tttatgacac
     4321 aacacaataa ggagttatgg atatgaaagg tataatgaaa tccattctgg ttttttccac
     4381 cgctgttttt ttagcagctt gttcatcgaa taaaagcggt tggacacgtt taaatgataa
     4441 tgatgttgat cagaaatctt atgctattgc ttatggtgca acggctcaga cttatgcgga
     4501 tagagtaaat gaaagctatg atatcaattc ctttatgaat ggggtaaatg attggtacac
     4561 taaaaaggtt acattgccaa ttgagcaaat tagagcaata acgattaatc gaatgttaga
     4621 ccataatgtg tatgcttatt atagtggtgt gatttatgcc gcagatttgc aggcaaattt
     4681 caatcattta gatcctaatt gttgggggct tgttcaacca gcgagtatga cccagggcat
     4741 tcacgatgcg atgttagatt tacagaagaa caaagttcgt ggagatgagt atatcaaaaa
     4801 tggggttgag caaatattgc acctttgtgt aaaaacgatt gctgaagatg aaaatgaatc
     4861 gcaatctaaa ggtaaaacca aaaagcataa ataagttaag ctatgacaag cggtaaaatt
     4921 tttaaaaaat tttaccgctt ttttataacc gtatgaaatg tttaatattt attctatgaa
     4981 acttaaaaaa acagcgtttt gtttattcaa taggcaaaca ctcgggattt ttcaattttc
     5041 aggaaaaaga atttgacatc tttttaaatt ctagtaatat ggcgcccaca gacacagtga
     5101 gtggtgagat ggccgagcag gctgaaggcg ctcccctgct aagggagtat agggttaaaa
     5161 agctctatcg agggttcgaa tccctctctc accgccattt actttatcac gcacccgtag
     5221 ctcagctgga tagagtactc ggctacgaac cgagcggtca gaggttcgaa tcctctcggg
     5281 tgcgccattt taatttttac tctattcaaa ttaaaatggt tgctgataaa attaaataaa
     5341 cttacaattc aaata
//
LOCUS       DANLYI010000163         5312 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.164, whole genome shotgun sequence.
ACCESSION   DANLYI010000163 DANLYI010000000
VERSION     DANLYI010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 5312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5312
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.164"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            33..2600
                     /gene="clpB"
                     /locus_tag="PXD81_002015"
     CDS             33..2600
                     /gene="clpB"
                     /locus_tag="PXD81_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HDL5538006.1"
                     /translation="MNFEKMTTKLQEALAESQSLAVGKDNPYIEPAHLLYALLKQEGG
                     SIASLFTTLNVDVPTLIRELQQILDRLPKVQGGNTQVSQQLVRLLNQSDKLAQQFGDS
                     FISSELFVLAALDDNGDLGKLFKQFGLNKEKLTQAISQIRGGDTVNNQNAEDTRQALK
                     KYTIDLTERAKAGKLDPVIGRDEEIRRAVQVLQRRTKNNPVLIGEPGVGKTAIVEGLA
                     QRIVNGEVPEGLKNKRVLSLDMGALIAGAKYRGEFEERLKAVLNDLAKEEGQVILFID
                     EIHTMVGAGKTDGAMDAGNLLKPSLARGELHCVGATTLDEYRQYIEKDAALERRFQKV
                     LVDEPTVEDTIAILRGLKERYEIHHHVQITDPAIVAAATLSHRYVSDRQLPDKAIDLI
                     DEAASSLRMEIDSKPEPLDKLERRIIQLKLERQALQKEEDEASRQRLAKLDEEMAAKE
                     REYSELEEIWKAEKSALLGTQHIKSELENARIAMDQARRENDFEKMSELQYGVIPNLE
                     KQLQAVENGEKNADAQLLRTKVTEEEIAEVLSKATGIPVAKMMEGEKEKLLRMEEVLH
                     ARVIGQNEAVDAVANAIRRSRAGLSDPNKPIGSFLFLGPTGVGKTELSKTLANFLFDD
                     PDAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRHPYSVVLLDEVEKAH
                     PDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSHLIRENAYKGYDVVKEMVM
                     EVVGQHFRPEFINRIDETVMFHSLGQEHIRSIAKIQLQRLIQRLAERGYEVTVTDAAL
                     DHIGKAGFDPLFGARPLKRAIQQELENPLAQQILSGKLLPNKAVTVDYQNGQIVATQ"
     gene            2814..3956
                     /locus_tag="PXD81_002016"
     CDS             2814..3956
                     /locus_tag="PXD81_002016"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HDL5538007.1"
                     /translation="MSQLFPEATIRTQAPYRNDIVGSFLRTDALKKAREDFNQQKITR
                     DELREVTRQEVKKLVELQKQHGVKAVSDGEFSRTWWHLDFLAELDGMDWQETEVFNVN
                     FTGHKPKGQTVKIVGDIDFSESHPFVDAYRLLKEAAGDYPTKFTIPAPTMLHIICCVR
                     AEDYQPLPQYQDEQKLYDDIANAYIKAMHKFYDMGLRNLQLDDTSWGQFCAEDKRKEF
                     AARGFNLTDLAEKYVALLNRIVDAKPADMSITMHICRGNFRSSWFSEGGYGPVAEVLF
                     GKCRVDGFFLEYDTERAGTFEPLRHIKDQQVVLGLITSKFGDLEDKNVIIERIKEAAQ
                     IVPINQLCLSPQCGFASTEEGNILTEAAQWAKLNLIKEIAEEVWGS"
     gene            complement(4013..>5312)
                     /locus_tag="PXD81_002017"
     CDS             complement(4013..>5312)
                     /locus_tag="PXD81_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DASS family sodium-coupled anion symporter"
                     /protein_id="HDL5538008.1"
                     /translation="TLPFTPEANKGLALLIFIAVLWLTEALHVTVTALLIPILAVALG
                     LVKSKEALVAFADPTIFLFFGGFALATALHIQQLDRMIANKIMALAKGKLSIAVIYLF
                     AVTAFLSMWMSNTATAAMMLPLAMGILSKLDQKQNHNTYLFVLLGIAYSASIGGMGTL
                     VGSPPNAIVASQLNLTFADWLKYGLPIMLILFPLMIATLYIVFKPNFSVSFDRSFEKI
                     ELNRSRIITLAIFVFIALGWIFGDKINPIISAFLGINGKIASFDTILALIAAALICIT
                     RVANWQQIQENTEWGVLFLFGGGLTLSAVLGQTGASKIMADGVVSLIEGGHFYLIGLI
                     VAAFIIFLTEVTSNTASAALLVPIFISIAQALNMPPVGLALIIGLGASCAFMLPVATP
                     PNAIVFGTGQIKQSEMIKVGFWLNIISIFVIATVGYLFWL"
BASE COUNT         1723 a         1091 c         1155 g         1343 t
ORIGIN      
        1 acactttcta aactcattgt aaggagaaag aaatgaattt tgaaaaaatg accacaaaac
       61 tgcaagaagc cttggctgaa tcacagtcgc ttgcggtagg aaaagataat ccgtacatcg
      121 aaccggcaca tttgctctat gcgttgttaa aacaagaagg tggctcgatt gcttctttgt
      181 ttaccacatt aaatgttgat gtaccgactt taattcgtga attacaacaa attttagacc
      241 gcttgccgaa agttcaaggc ggtaacaccc aagtttcaca acagttggtc agactgttaa
      301 atcaatcaga caaactggca caacaattcg gcgacagctt tatttcatca gaactatttg
      361 ttctagctgc acttgatgac aatggcgatt tggggaaact atttaaacaa tttggcttaa
      421 acaaggaaaa actaacacaa gccatcagcc aaattcgagg aggagacaca gtgaataatc
      481 aaaatgcgga agatacccgc caagcactca aaaaatatac gattgattta accgaaagag
      541 ccaaagccgg taaattagac ccggttatcg ggcgtgatga agaaattcgc cgtgcggtgc
      601 aagtcttaca acgccgtacc aaaaacaacc ctgtgttaat tggtgaaccg ggtgtgggga
      661 aaacggcgat tgtagaaggc ttggcacagc gtattgtaaa cggcgaagtg ccggaaggct
      721 tgaaaaataa gcgtgtactt tcgctggata tgggagcatt aattgccggt gcgaaatatc
      781 gtggtgaatt tgaagagcgt ttaaaagcgg ttttaaacga cctggcgaaa gaagaagggc
      841 aagttatcct ctttattgat gaaattcaca cgatggttgg agcaggcaaa acagacggag
      901 caatggacgc cggcaactta ttaaaaccaa gccttgcccg tggtgaattg cactgtgtgg
      961 gggctacaac ccttgatgaa tatcgccaat acattgaaaa agacgcagcc cttgaacgcc
     1021 gtttccaaaa agtattggtg gacgaaccaa cggttgaaga tacgattgcg attttacgtg
     1081 gtttgaaaga gcgttatgaa atccatcatc acgttcaaat tacagaccct gccatcgtgg
     1141 cggcggcaac gctttcgcac cgttatgtgt ctgaccgtca attgccggat aaagcgattg
     1201 acttgattga tgaagccgct tcaagcctgc gtatggagat tgattctaaa cctgagccgt
     1261 tggataaatt agaacgccgc attatccaac tcaaattgga acgccaagcg ttacaaaaag
     1321 aggaagatga agcgagccgt cagcgtttag ccaaattaga cgaagaaatg gcagcgaaag
     1381 agcgtgaata ttccgagctg gaagaaatct ggaaagcgga aaaatccgcc ttacttggta
     1441 cacagcatat taaatcggag ttggaaaatg cccgaattgc gatggatcag gctcgccgtg
     1501 aaaacgattt cgagaagatg tcggaattgc aatatggcgt gattccaaac ttggaaaaac
     1561 agttacaagc ggtcgaaaat ggcgaaaaaa atgcagatgc tcagctcctc cgtactaaag
     1621 tcaccgaaga ggaaattgcg gaagtgcttt caaaagcaac gggcattcct gttgcaaaaa
     1681 tgatggaagg cgagaaggaa aaactccttc gtatggaaga agtattacac gctcgggtta
     1741 tcgggcaaaa tgaagccgtt gatgcggtgg caaatgcgat tcgccgtagc cgtgccggac
     1801 tttccgatcc gaataaaccg attggttcat tcctattctt aggaccgacc ggcgtgggta
     1861 aaaccgagtt aagcaaaacc ttagctaatt tcttgtttga cgatcctgat gctatggtgc
     1921 gaattgatat gtctgaattt atggaaaaac acagcgtatc ccgcttagtg ggcgcacctc
     1981 cgggctatgt cggctacgaa gaaggcggct atttgaccga agcggttcgc cgccatccat
     2041 attcggtagt gttacttgat gaagtggaaa aagctcaccc tgatgtgttt aacattttat
     2101 tgcaagtgtt agatgacggg cgtttaaccg acgggcaagg cagaacagtc gatttccgca
     2161 atacggtagt gattatgacc tcaaacttag gctcgcattt aattcgagaa aatgcgtata
     2221 aaggctatga tgtggttaaa gaaatggtga tggaagtcgt cggacagcac ttccgccctg
     2281 aatttatcaa ccgtattgat gaaacggtga tgttccactc acttggtcaa gagcatatcc
     2341 gttcgattgc gaagattcag ctacaacgct taatccaacg cttggcagaa cgaggctatg
     2401 aggtgacagt aaccgatgca gcattagatc atatcggcaa ggctggtttc gatccgctgt
     2461 tcggagcaag accactgaaa cgtgcgattc aacaagagct tgaaaatccg cttgctcaac
     2521 agatcttaag cggtaaatta ttaccgaata aagccgttac ggtggattac caaaacgggc
     2581 agattgttgc aacacaataa tgtctccgat ggtgcgagcg tcctcgctcg taccttacaa
     2641 gcggtggatt tatcgtaaaa atttgcaaaa aattgctcga aaataaccgc ttgcacagta
     2701 aaaattttca tattgccctt cattaatttt cataagctaa tgaagggctt tttattactc
     2761 tttttgctat cattaacggc gtttataacc aatacaatta caaggagtaa tttatgagcc
     2821 aattatttcc ggaagcaaca atccgcactc aagccccgta tcgtaatgat attgtaggca
     2881 gctttctacg taccgatgcg ttaaaaaaag ccagagaaga tttcaatcag caaaaaatca
     2941 cccgtgacga attgcgtgaa gtcacacgcc aagaggtcaa aaaattagtt gagttgcaaa
     3001 agcaacacgg tgtaaaagca gtctctgacg gtgaatttag ccggacttgg tggcatttag
     3061 atttcttagc tgaacttgat ggaatggatt ggcaagaaac cgaagtcttt aacgtgaact
     3121 ttaccggtca taaacccaaa gggcaaacgg taaaaatcgt gggcgatatt gatttctccg
     3181 aaagccaccc ttttgtggac gcttatcgtc ttttaaaaga agctgccggc gattacccaa
     3241 ccaaattcac gattccggca ccgacaatgt tacatattat ctgctgtgtg cgtgcggaag
     3301 attaccaacc gttaccacaa tatcaagacg aacaaaaact gtacgatgat attgctaatg
     3361 cttatatcaa agcaatgcac aaattctacg atatggggct acgtaactta caattagacg
     3421 acaccagttg gggacaattc tgtgccgaag ataaacggaa agagtttgca gccagaggat
     3481 ttaatttaac tgatttagcg gaaaaatatg tcgcattatt aaaccgtatt gttgatgcaa
     3541 aaccggcgga tatgtcaatt acgatgcaca tctgccgtgg taacttccgt tcttcttggt
     3601 tctcggaagg cggttatgga ccggttgccg aagtattatt cggtaaatgc cgtgttgacg
     3661 gcttcttctt agaatacgat accgaacgtg caggcacttt cgagccacta cgccacatta
     3721 aagatcaaca agtggtacta ggcttaatta cctctaaatt tggcgattta gaagataaaa
     3781 atgtgatcat tgagcgtatt aaagaagcgg ctcaaattgt gccaatcaat cagctatgct
     3841 tatcaccaca atgcggcttt gcctccaccg aagaaggcaa tattttaacc gaagccgcac
     3901 aatgggcgaa actaaactta attaaagaaa ttgcggaaga agtttggggt agctaatcct
     3961 ctataataca aatagccgag taaatactcg gctattttag tttgttttaa cattacaacc
     4021 agaagagata cccaacagtc gcaatcacaa agatagagat aatatttagc cagaaaccaa
     4081 ctttaatcat ctcactttgt ttaatttgcc ctgtaccaaa tacaatcgca ttcggtgggg
     4141 tggcaaccgg caacataaag gcgcaagatg ctcctagacc aataattaag gctaatccga
     4201 ccggtggcat atttaatgct tgtgctatag agatgaaaat cggcactaac aaagccgcac
     4261 tggctgtgtt agaggtaacc tcagttagga aaataataaa tgctgccaca attaaaccaa
     4321 ttaggtaaaa atgcccccct tcaattagtg agacgacccc atcagccata attttgctcg
     4381 caccagtttg tcctaataca gcacttaacg tcaaaccacc accaaatagg aataataccc
     4441 cccactcggt gttttcttga atctgttgcc aatttgcgac acgagtaatg caaataagtg
     4501 ctgcggcaat caatgcaaga atggtgtcaa agctcgcaat tttgccatta attcccagaa
     4561 atgcagaaat aatagggtta attttatcac caaaaatcca accaagcgca ataaagacga
     4621 aaatagccaa ggtgataata cgagaacgat ttaattcaat tttctcaaat gatcgatcaa
     4681 aactaactga aaaatttggt ttaaatacga tatatagtgt tgcaatcatt aatgggaata
     4741 aaattagcat aattggcaaa ccatatttta accaatcagc aaaagtcaga ttcaattgag
     4801 aagcaacaat cgcattcggt gggcttccca ctaaagtacc cattccacca atactggcac
     4861 tataagcaat tcctaataac acgaaaagat aagtattatg gttttgtttc tgatcaagtt
     4921 tacttaaaat ccccattgcc aaaggtaaca tcattgcagc tgtagctgta ttactcatcc
     4981 acattgacaa aaacgctgtt accgcaaata aataaataac tgcaatcgat aatttacctt
     5041 tagctaatgc cataatttta ttggcaatca tacgatctag ctgctgaatg tgtaatgctg
     5101 tcgctaaggc aaagcctcca aaaaataaga aaatcgttgg atcagcaaaa gcaacaagag
     5161 cttcttttga tttaaccagc cccagtgcta cagccaaaat cggaattaat aatgccgtaa
     5221 ccgtaacgtg taatgcttct gttagccata agacggcaat gaaaattaac aatgccaacc
     5281 ccttattggc ttcaggggta aaaggcaagg ta
//
LOCUS       DANLYI010000164         5311 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.165, whole genome shotgun sequence.
ACCESSION   DANLYI010000164 DANLYI010000000
VERSION     DANLYI010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 5311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5311
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.165"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            complement(<1..369)
                     /locus_tag="PXD81_002018"
     CDS             complement(<1..369)
                     /locus_tag="PXD81_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HDL5538009.1"
                     /translation="MKFKDEQTEQKLRGGYYTPQYLADYITKWVLGINPSSILEPSCG
                     DGRFIQALFNNNSEKEKNITCFELIDSEADKSKYLLKSLGFNNFSVYSSDFLRWSVDN
                     FKADQIEFEGIIGNPPFIRYQ"
     gene            complement(583..1149)
                     /locus_tag="PXD81_002019"
     CDS             complement(583..1149)
                     /locus_tag="PXD81_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5538010.1"
                     /translation="MEGLIMGLQLIPHFPELAISRRTYLHDMPMYCFVSKNWIFSAKD
                     RAKREALGNLLACGGEMMKSFIFKRSLFSEIWFEDILLEDEEKELIRILYNKPEPEYQ
                     VEEKIDWEAREARRKQADYDKWQSYDERNPFHYQIFPTDTIAYAQQQVEKFIEDVDNF
                     LEWFYDDEVKRYILERNEIYQKKFDLVN"
     gene            complement(1682..2008)
                     /locus_tag="PXD81_002020"
     CDS             complement(1682..2008)
                     /locus_tag="PXD81_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL5538011.1"
                     /translation="MLHSKGKILPKITLNEIETKERFAIALRKEMHAQYWTGKMVANA
                     LDVSEKTVKDWVAGKRLPSSLDLLALMRISSCVRQLVWDISKSEECYQDAEMLKQICF
                     VLLSNL"
     gene            2056..3687
                     /locus_tag="PXD81_002021"
     CDS             2056..3687
                     /locus_tag="PXD81_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDL5538012.1"
                     /translation="MVTRTKNQPKNQAVSYIRMSTEHQEFSPDLQRCFIENYAKENGL
                     EIIREYIDEGKSGLTAEHRPQFLSMIQLVQSGQADFDHILVYDVSRWGRFMNIDESGH
                     YELICTYQGIKVHYCAEPFKGNDIASQIYKTMKRLSVGDYCRELGVKVFNGQKNLIQH
                     GFRQGGVAGFGLRRRLIDCQGNPKFDLQRGDRKSLQTDRVILVAGPKEEQEIVRQIYH
                     DFVYQHKTEQQIADSLNAQGIVTDRNTAWTKGVVHQILINEKYVGHNVWNKRSASKTR
                     EYKGKNPVEEWVRAENVFEAIVPQALFNAAQTIIHQRSARLSDDEMLEQLKLLLKEKG
                     KLSGLIIDEAENCPSSSAYSSRFGSLLNTYTLINYEPERDYHYVEINRLLRQQHKNFV
                     QQTLDTIIELGGVVLSDDVTDLVHINNEFTASIVLSRCRLTPSGSKRWLIRFDTSLNP
                     DITIAVRINESATEILDYYLLPTTEKVNEKLRLAESNPAELEIYRHDNLDRFFIMVER
                     ILVKEFIYAKRHHSNSSYRQNSGTQSQNAQSESLC"
     gene            3602..4495
                     /locus_tag="PXD81_002022"
     CDS             3602..4495
                     /locus_tag="PXD81_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HDL5538013.1"
                     /translation="MQNDTIQILPIDKIRVLNPRTRNQKVFADLVENIATIGLKRPIT
                     VTPSVDSAEYYDLLCGQGRLEACQVLGELFIPCRVVNANSEESYLISLVENIARRQHS
                     YMELLSGIKILNDRGYKPVDIARKIGFSKSHVNSILHLINAGEERLINAVEQGILPIT
                     IAISISRSDDQEVQKQLAELYTNGTLKSTDIAKIRNLIHRRNLAGKKANTGISGSIRS
                     SIYNPKTVVNIYKEETERQKMMIKQADYDEKQLSIILSCLNKLFEDKYFQLVLKSEHL
                     DDMPKDLSQRMLKQREGNIYA"
     gene            4488..>5311
                     /locus_tag="PXD81_002023"
     CDS             4488..>5311
                     /locus_tag="PXD81_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HDL5538014.1"
                     /translation="MPKHITYAFHNQLISLPIDEIIPLKKMDKNFEKSTKFQSILASI
                     REIGIVEPVVVYPEENNQYILLDGHSRVHALKKLGVDKVLCMISTDDEGFTYNKQINR
                     LTAVQENKMLMKTIERGVSDELIARTLNINLKALRQKMNMLNGITREVVDILANKQVS
                     KEIFRILRKMKPERQIEVAQMMIASNKFTLTYANMMLLSSRKEELVESHKSKTTNDDL
                     TDLSIMQKELERLKENYKISEEKLADLKMGLVVAKGYVKKLLNNAAVVDLLENDQS"
BASE COUNT         1758 a         1011 c          987 g         1555 t
ORIGIN      
        1 ctgatagcga atgaaagggg ggtttccaat tatcccctca aactcaattt gatcagcttt
       61 aaagttatca actgaccatc ttaaaaaatc actactgtaa actgaaaaat tattaaatcc
      121 caatgacttt aatagatatt ttgatttatc tgcttctgaa tcaattagtt caaagcaagt
      181 tatattcttt tctttttcgc tattattatt aaatagagcc tgaataaacc gaccatcacc
      241 acaactaggc tctaatatgc tagatggatt aattcccaaa acccattttg taatataatc
      301 tgctaaatat tgaggggtat aatatcctcc ccttaacttt tgttctgtct gttcatcttt
      361 aaatttcatt gttaataact aattttccta tatgtatttc taccacaata tcactttcaa
      421 tatatctttg gtatataatt aaatcacttt aataaataaa acaactatct attaaaatga
      481 atagttgtat tctaacagta gttaccctaa atctacaaat atcaagtgta aaaaaatata
      541 aaagtcggca ataacattat tttataataa ctaatgtgaa ttctagttca ctaaatcgaa
      601 tttcttttgg taaatctcat tcctttctaa tatatagcgt tttacctcat catcgtaaaa
      661 ccattcaaga aagttatcaa catcttcaat gaacttttct acctgttgct gtgcatatgc
      721 tatcgtatcg gtagggaaga tttgataatg aaacggattg cgttcatcat aactctgcca
      781 tttgtcataa tcggcttgtt tacgccgcgc ttctcttgcc tcccagtcga ttttttcttc
      841 cacttgatat tctggctctg gcttattata caaaatacgg atgagttcct tttcttcatc
      901 ctcaagcaga atatcttcaa accagatttc agaaaatagg ctgcgcttga aaatgaaaga
      961 cttcatcatt tcccctccac acgccaataa gtttcccaat gcttcccgtt tcgctctatc
     1021 ttttgccgaa aatatccagt ttttcgatac aaaacagtac attggcatgt catgcaaata
     1081 ggttcttcgt gaaatggcta attcaggaaa atgaggaata agctgtaacc ccattattaa
     1141 gccttccata ctgcacacga tcttacttaa ttgtccgtaa tcgcgtaaaa aagcaatgtc
     1201 agtttttatc aaccacaacg ataggctgac taatatgaac ataaagatga gataatagat
     1261 cggttttaag gctaaggcgc gccgacaaag ccacattaag agtggaatcg taacgagatc
     1321 aaagaaaaat gatgagtcta tgatgatttc cttatgtgag ttgatgaata atcaggcatg
     1381 aaggaaacct taaaaaggaa aagtcccttc attaccactt tttaaaacct tttaaggctt
     1441 aaaaatcaaa agtcgtaatg aagggactat tgtccgtggg caaaaaggta ttagttatag
     1501 ggggctttgt caagcgaaaa tttactgtgc attttctcgc caaaactgat ttaagccttg
     1561 ataaaatctc gaaagtacct tgtcaagaaa aatcttttta gtggtttgat tgctagtttt
     1621 ttgagcaaaa ctatgctgta atgcagtaaa aatacagttt ctatctgtgt ttggaatcca
     1681 gttacaaatt ggagagtaat acaaagcata tttgcttgag catctcagca tcttggtagc
     1741 attcttctga ttttgaaata tcccaaacaa gctgccgtac acatgatgaa attctcatta
     1801 acgccaataa atctaggcta cttggcaatc ttttccctgc aacccaatct ttaactgttt
     1861 tctcactcac atctaatgca ttggcgacca ttttccccgt ccaatattga gcatgcattt
     1921 cctttcgtaa cgcaattgca aaccgctctt tcgtttcaat ctcattgagt gtaattttgg
     1981 gtaagatttt accctttgag tgcaacatat ttcctcccga ttcctttata ttggtgtaga
     2041 taaggagtca ttagaatggt tacacgtaca aagaatcaac ctaaaaatca ggcggtttcc
     2101 tacattcgaa tgtcaacgga acaccaagaa ttttcaccag accttcaacg ttgctttatc
     2161 gaaaattatg ctaaagaaaa tgggctagaa atcattagag aatacattga tgaaggaaaa
     2221 agtgggctaa ccgcagaaca ccgcccacaa ttcctttcaa tgatacaact cgtacaaagt
     2281 ggacaagccg acttcgatca tattttggtg tatgacgtga gtcgttgggg tcggtttatg
     2341 aatattgatg aatcggggca ttatgaactg atttgtacct accaaggcat taaagtccac
     2401 tattgcgccg agccattcaa agggaatgat attgcctctc aaatatataa aaccatgaaa
     2461 cgtttgtcgg taggagacta ctgtcgtgaa ctcggagtga aagtatttaa cggtcaaaaa
     2521 aatctgatcc aacatggatt tagacaaggt ggggtagctg gatttggatt gcgtaggaga
     2581 ttgattgact gtcaaggaaa ccctaaattt gatctccaac gtggagatag aaagagccta
     2641 caaaccgaca gagtgatttt ggttgcaggc ccaaaggaag agcaggaaat tgtccgacaa
     2701 atttaccatg attttgttta tcaacataaa acggaacaac aaattgctga tagcttaaat
     2761 gcacaaggca ttgttactga tagaaataca gcatggacga aaggcgtagt acatcaaatc
     2821 cttattaacg aaaagtatgt cggccacaat gtttggaata aacgtagtgc atcaaaaaca
     2881 cgcgaatata aaggtaaaaa ccccgtagaa gagtgggtgc gagctgagaa tgtatttgaa
     2941 gctatcgtgc cacaagcctt atttaatgca gcccaaacca ttattcatca acgctccgcc
     3001 cgattatctg acgatgaaat gttagagcaa ctcaagctac ttttgaaaga gaaagggaag
     3061 ctgtctggct tgatcattga tgaagccgaa aattgtccat caagtagcgc gtacagtagc
     3121 cgatttggaa gcctacttaa cacatatact ttaattaact atgaacccga acgggattac
     3181 cactatgtag agatcaaccg attactccgt cagcagcata aaaatttcgt acaacaaaca
     3241 cttgatacca ttattgaatt ggggggagtg gttttgagcg atgatgtaac cgatttagtc
     3301 cacatcaata atgaatttac cgcctcaatc gtattaagtc gttgtcgcct aacgccatca
     3361 ggaagcaaaa gatggttgat ccgatttgat accagtttaa atcccgacat aacaattgca
     3421 gtgcgcatca atgagagtgc cacagaaatt cttgattact acctgcttcc cacaactgaa
     3481 aaggtaaatg aaaaactacg attggctgag agtaatcctg ccgaacttga aatctaccgc
     3541 catgataatt tagatcgctt ttttattatg gttgaacgta ttttagtcaa ggagtttatc
     3601 tatgcaaaac gacaccattc aaattcttcc tatcgacaaa attcgggtac tcaatcccag
     3661 aacgcgcaat cagaaagtct ttgctgatct ggtcgaaaac attgcaacga ttggtctcaa
     3721 gagacctatt accgtaacgc catctgtcga tagtgctgaa tattatgatt tgttatgcgg
     3781 tcaaggtcgg cttgaggctt gtcaggtgct tggtgagcta ttcatacctt gtcgcgttgt
     3841 gaacgcaaat agtgaagaaa gctacttgat cagccttgtg gaaaatattg ccagacgtca
     3901 gcattcctat atggagctgc tttcaggcat caaaatactc aatgatagag gttataagcc
     3961 cgttgatatt gctcgcaaaa tcggtttctc aaaaagccat gtcaacagta tcttacacct
     4021 catcaatgct ggggaagagc gccttatcaa tgcggtagag caaggaattt tacccattac
     4081 catcgctatt tcgatttctc gttcagacga tcaggaagtt caaaaacaac tcgcggaact
     4141 ttacaccaat ggtacattga aaagcaccga tattgccaaa atacgtaacc tgatccaccg
     4201 tagaaatctt gctggtaaga aagccaatac aggtatttct ggaagtatta gatcatctat
     4261 ttataatccc aaaactgtgg tgaatatcta caaagaggaa acggaaagac agaaaatgat
     4321 gatcaagcaa gcggactatg atgaaaaaca actctctatt atcttgtctt gcttaaataa
     4381 actctttgag gacaaatact ttcaactcgt ccttaaatca gaacatttag acgatatgcc
     4441 aaaagattta agccaacgaa tgttaaaaca acgagaggga aatatttatg cctaaacata
     4501 ttacttatgc ctttcataat cagttaattt cgcttccaat tgatgagatt attccattaa
     4561 aaaagatgga taaaaatttt gagaaaagca ctaagttcca aagcattctg gcttcaattc
     4621 gtgaaatcgg gattgttgaa cctgtggtcg tctatccaga agaaaacaac caatatattt
     4681 tgttagatgg ccattctcgt gtacatgcac tcaaaaagct aggtgtggat aaggtgcttt
     4741 gtatgatttc taccgatgat gaaggcttta cttacaacaa gcaaattaac cgcttaaccg
     4801 ccgttcagga aaataaaatg ctgatgaaaa ccattgagcg cggggtttcg gatgaactga
     4861 tcgcaagaac actcaatatc aatctaaaag cactacggca gaaaatgaat atgcttaatg
     4921 ggatcacaag agaggttgtt gatatattgg ccaataagca ggtcagtaaa gagatatttc
     4981 ggattttgcg caagatgaag cctgaacgcc aaattgaagt ggcacaaatg atgatcgcca
     5041 gtaataagtt tacattgaca tacgccaata tgatgttgct cagttcacgc aaggaggaat
     5101 tagtcgaaag ccacaagtct aaaaccacga atgatgattt aaccgatctt tccattatgc
     5161 aaaaagagct agagcgactt aaagagaact ataaaatttc cgaagagaaa ttggcggact
     5221 taaaaatggg attggttgtg gcgaaagggt atgtcaaaaa actattgaat aatgctgccg
     5281 tggtagattt gcttgaaaat gatcagagtg a
//
LOCUS       DANLYI010000165         5172 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.166, whole genome shotgun sequence.
ACCESSION   DANLYI010000165 DANLYI010000000
VERSION     DANLYI010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 5172)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5172)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5172
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.166"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            complement(<1..518)
                     /locus_tag="PXD81_002024"
     CDS             complement(<1..518)
                     /locus_tag="PXD81_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015587106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HDL5538015.1"
                     /translation="MLDSLKEVNQKLDKKADKVGGGTKEDALVTLDTLIASGTVKAET
                     AMSLLPTLQKGAVATGASSEDMAKIAISSMQQFGIKEEDIGRALDMAVAAGQAGSFEL
                     AYMASWLPQQMAAAKQAGLSSIEGFERLLIANQQARVTAGTSDEAGNNLVNLLGKITA
                     KETNERFKNIEY"
     gene            617..868
                     /locus_tag="PXD81_002025"
     CDS             617..868
                     /locus_tag="PXD81_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL5538016.1"
                     /translation="MAPTISDGDQMLVDFSKKEARGDDKIYLVQNGESVWVKRVRKVW
                     DYVELISDNESYRPIRITADEAQNLQIIGQVVHIGHSLV"
     gene            complement(1064..1264)
                     /locus_tag="PXD81_002026"
     CDS             complement(1064..1264)
                     /locus_tag="PXD81_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HDL5538017.1"
                     /translation="MQYVLDLTGYACPLPLLMAKKAMNDLKKGDSLEILLNQQSSLTD
                     FELLAKEQGWLCELQAVENVGY"
     gene            complement(1323..1835)
                     /locus_tag="PXD81_002027"
     CDS             complement(1323..1835)
                     /locus_tag="PXD81_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017357669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HDL5538018.1"
                     /translation="MQQLEPQTFASWAEPIDMLYACHSKVKSFCKQLQILPDYLEKNG
                     VNQAVKNDVQQILNYFNISAPLHHEDEEDDFFPELIKVQPQAQAEVDELERQHVHLHK
                     NWDYLSAQLEDLLAGKRENVDRDLIERFVAGYDVHIAIEEPLFELGREHLAQDKLSAM
                     GKVMADRRKL"
     gene            complement(1848..3002)
                     /gene="tgt"
                     /locus_tag="PXD81_002028"
     CDS             complement(1848..3002)
                     /gene="tgt"
                     /locus_tag="PXD81_002028"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HDL5538019.1"
                     /translation="MKYELKTTSGNARRGRLTFNRPKGEFHVETPAFMPVGTYGTVKG
                     MTPEEVKATGAQILLGNTFHLWLRPGQEVMKMHGDLHDFMQWHGPILTDSGGFQVFSL
                     GKLRKIKEEGVTFQNPISGEKIFLSPEKSMEIQYDLGSDIVMIFDECTPYPATFDYAK
                     KSMEMSLRWAKRSRDRFDELENPNALFGIIQGGIYEELRKVSVEKLVEIGFDGYAVGG
                     LAVGEPKEDMHRILEYVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNA
                     RNGHLFVTNGIVKIRNAKYRADTTPLDPECDCYTCKNYTKAYLYHLDKCGEILGARLN
                     TIHNLRYYQRLMAEIRQAIEEDRFDDFVVEFYARIGKEVPPLQSEVKEQK"
     gene            complement(3061..3201)
                     /locus_tag="PXD81_002029"
     CDS             complement(3061..3201)
                     /locus_tag="PXD81_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5538020.1"
                     /translation="MKKLLLCVGILVLAACSSTGVGGSIGAGGGSNGVGIGLGVGTDF
                     RF"
     gene            3295..3867
                     /locus_tag="PXD81_002030"
     CDS             3295..3867
                     /locus_tag="PXD81_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1439 domain-containing protein"
                     /protein_id="HDL5538021.1"
                     /translation="MKILRKFAAVCVVSMAMLSANANLLTISEQEINDYLATKLAEKI
                     PLKDKVGIPKLFELDYRLHSLTSQIGRTEEKKVAISGVVDGLLNAKGKKYAASIHLNM
                     DTTPYYDPQKGALFLKDVRLLSWSATPAKYQAELQMFLPVLADGLSSFLNSTPVYTLD
                     ENKTKEAMVKKFGKAIVVENGNIKLETTIF"
     gene            3969..4187
                     /gene="cspD"
                     /locus_tag="PXD81_002031"
     CDS             3969..4187
                     /gene="cspD"
                     /locus_tag="PXD81_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein CspD"
                     /protein_id="HDL5538022.1"
                     /translation="MDIGIVKWFNNVKGFGFITSDVCEGDIFAHFSEIQAEGYRSLKV
                     GQKVQFELVQGERGASASNITPISEKTL"
     gene            4277..>5172
                     /locus_tag="PXD81_002032"
     CDS             4277..>5172
                     /locus_tag="PXD81_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDL5538023.1"
                     /translation="MPSSYQLVLINLLYIFGIGLNIVLLRFLSLHFDPLNNNGIRFLV
                     GGFTLLFFILWRFRTSLSQLIKTPKLIAISLFVGLMMSANMYFWLKGVKITNAVTVSI
                     FGVLAMPFGVIIAAIFFQDERERIKSKAFWFGSILTIIGSLGFIWHGKTLSIGEGFIL
                     GSVFLFLSIIIRNIQNLVVKFARELNVLTLSCVTSLTTSLSSLLLSQQAGKFNELFTA
                     PNWLLFSLMVAGIYAIMIGMVLTFHIIQTQGLITYQVLELLMPVSTAIIAYILLDERI
                     SIVQMGFACIVILGSSIALKLV"
BASE COUNT         1482 a         1095 c          957 g         1638 t
ORIGIN      
        1 ttatattcaa tatttttaaa acgctcattt gtttctttag ctgtaatctt accaagcaga
       61 tttaccagat tattacctgc ctcatcgctt gtaccggcag tgacacgagc ctgttggttt
      121 gcaattaaaa gtcgctcaaa gccctcaata ctactcaaac cggcttgttt tgcagctgcc
      181 atttgttgag gcaaccaact tgccatatat gcaagctcaa aactacctgc ttgcccggct
      241 gcaacagcca tatccaatgc ccgcccaata tcctcttctt tgatgccaaa ttgttgcata
      301 gaggagatcg caatttttgc catatcctcc gagcttgccc ctgttgccac cgcccctttc
      361 tgcaaagtag gtaatagact cattgcggtt tcggctttga ctgtaccgga ggcaatgagc
      421 gtatcaagcg taactaacgc atcctctttt gtgccacccc caaccttatc cgccttttta
      481 tcaagttttt ggtttacttc tttgagcgag tctaaaatat cgtccagctt ttccttaaga
      541 gccttattca gacagcctat taagcctagg cgtaaaagcc cataattgtg gcgtattttg
      601 ggctaatggc acatcaatgg ccccgactat cagcgatggt gatcaaatgt tagtggattt
      661 cagcaaaaaa gaagcccgtg gagacgataa gatttatctt gtgcaaaatg gtgaaagcgt
      721 ttgggtaaaa cgtgtaagaa aagtgtggga ctacgttgaa ttgattagcg ataatgagtc
      781 ctaccgccca atccgaatta cagcagacga agctcaaaac ttacaaatca tcgggcaggt
      841 agtccacatc ggacactctt tagtttaaaa cagatttaaa atagttttaa aaccgatcaa
      901 acctatgcaa aaaatatcgc aaaaactgcc catttttttc taatcctgct cattttgttt
      961 tttgcatagc cccaaccaaa caataacggc gaacccttgt attcacgggg ctcgccgtta
     1021 tttttccgcc caaatttttt tctttctctc tatgcaaaaa agatcagtac cccacatttt
     1081 caaccgcttg tagctcacaa agccaacctt gctctttggc aagcagttca aaatcggtaa
     1141 ggctactctg ctgattgagt aaaatttcca agctatcgcc ctttttaaga tcgttcatcg
     1201 ctttttttgc cattaataac ggcaaggggc aggcatagcc ggttaaatct aaaacatatt
     1261 gcatagcgaa atagggaata aagaaaaagg gcgaatattg aaattcgccc aatcacaaaa
     1321 gattagagtt tacggcggtc tgccatcact ttgcccatag ccgataattt gtcttgagct
     1381 aaatgctctc gcccaagctc aaagagtggc tcttcaatcg caatatgcac atcataacct
     1441 gccacaaaac gctcaattaa gtctctatcc acattttcac gtttacctgc gagcagatct
     1501 tcaagctgtg ccgacaaata gtcccaattt ttatgtaaat gaacgtgctg gcgttccagt
     1561 tcgtctacct ctgcttgagc ttgtggttgc accttaataa gctcagggaa gaaatcatct
     1621 tcttcatctt cgtgatgtaa aggggcagaa atgttgaagt agttcaaaat ctgctgcacg
     1681 tcattcttca ctgcttggtt taccccgttt ttctcaaggt aatccggcaa aatttgcaac
     1741 tgtttacaga aactttttac tttgctgtgg caagcataaa gcatatcaat cggctctgcc
     1801 cagcttgcaa aggtttgtgg ttcaagttgt tgcatataca ctccttgtta tttctgttct
     1861 tttacttcag attgtaaagg tggcacttct ttgccaattc gggcataaaa ttccactaca
     1921 aaatcatcaa aacgatcttc ttcgatggcc tggcgaattt ctgccattag gcgttgatag
     1981 tagcgtaaat tatgaatggt gtttagtctt gcccctaaaa tctcgccaca tttgtctaag
     2041 tgatataaat aggctttggt gtagtttttg caagtgtaac aatcacattc aggatcaagc
     2101 ggtgtggtgt ctgcacgata tttcgcatta cgaattttta caatgccgtt ggtcacaaat
     2161 aaatgtccgt tacgggcatt gcgggttggc attacgcagt caaacatatc aataccacgg
     2221 cgaacccctt cgactaaatc ttctggcttg cctacaccca ttaaatagcg tggtttatct
     2281 gccggaattt gcgggcaaac gtattctaaa atgcggtgca tatcttcttt aggttcacct
     2341 accgccaagc caccgactgc atagccatca aagccgattt caactaattt ttccaccgag
     2401 actttgcgaa gttcttcata aataccacct tgaataatgc caaataacgc attcgggttt
     2461 tcaagttcat caaagcgatc acgagaacgt ttcgcccaac gtagtgacat ttccatcgat
     2521 ttttttgcat aatcaaaggt tgccggataa ggagtacatt catcaaaaat cattacaatg
     2581 tctgatccaa gatcgtattg gatctccatt gatttttccg gcgaaaggaa aattttctca
     2641 ccgctaatcg ggttttggaa agttacgcct tcttctttaa ttttacgcag tttacctaag
     2701 ctaaaaactt ggaaaccgcc ggagtcggtt aaaatagggc cgtgccattg cataaaatcg
     2761 tgcaagtcac cgtgcatctt catcacttct tgccccggtc ttaaccataa atggaaagtg
     2821 ttgcctaata aaatttgcgc ccctgtcgct ttaacttctt caggagtcat tccttttacc
     2881 gtgccataag tgccaacagg cataaatgcc ggtgtttcaa cgtgaaattc gcctttcggg
     2941 cggttaaaag ttaaacgccc acgacgagcg ttaccacttg ttgtttttaa ttcgtacttc
     3001 attttaatcc acaaataaac agttcgtgaa agcggtcaaa aaccaccaaa aatttaccaa
     3061 ttagaatcga aagtcggtgc cgacccctaa gccgattcca acgccgttag agccaccacc
     3121 ggctccaata ctgccaccca caccggttga tgagcaagca gctaacacta aaatgccaac
     3181 gcataataag agttttttca ttttttgctg tcctaaacga aaaagagagt ctatgataga
     3241 cccaatgtat tctaacctgt ttaatggatt aaacctaatt ttaaagggta aattatgaaa
     3301 attttacgta aattcgctgc tgtatgcgtt gtctcaatgg ctatgctctc tgctaacgct
     3361 aatttgctca ctattagcga gcaggaaatc aacgactatt tagccaccaa attagctgaa
     3421 aaaattcctt taaaagataa agtcggtatt cctaaattat ttgaattgga ttatcgcctg
     3481 cattcactta ccagccaaat tggcagaacc gaggaaaaaa aagtagctat ttctggcgtt
     3541 gttgatggac ttttaaacgc aaaaggcaag aaatatgctg cctctatcca tttaaatatg
     3601 gataccacgc cttattatga tccacaaaaa ggggcattat ttcttaaaga tgtcagattg
     3661 ttaagttggt ctgcaacacc ggctaaatat caagcagagc tgcaaatgtt tttgccggtg
     3721 ttggctgatg gattaagtag ttttttaaac agtacgcctg tttatacttt agatgaaaat
     3781 aaaacaaaag aagcaatggt gaaaaagttc ggcaaagcca ttgtagtaga aaatggtaat
     3841 atcaagctag aaaccactat tttttaaata attttgtgat cttcgtctaa tttttacact
     3901 ttgatagttg agcttttacg cgttttattt acaatccaaa tatcgccatt cattatttgg
     3961 agagttctat ggatatcggt attgttaaat ggtttaacaa tgtaaaagga tttggtttta
     4021 ttacatcgga tgtatgtgaa ggagacattt ttgctcattt ttctgaaatt caagcagaag
     4081 gttatcgctc tttaaaagtc ggccaaaaag tacagtttga attagttcaa ggagagcgag
     4141 gtgcctcagc ctcaaatatc acgcctataa gcgaaaaaac gttataatcc gtcgttaata
     4201 aaattattgt tataaccata ctttattaga ttttaaagct cagcttttac agctgagcca
     4261 tttttgcttt ttgaatatgc cttcctctta tcaattagtc ttaattaacc tactttatat
     4321 cttcggtatt ggtctaaata ttgtactgct taggtttctc agtttgcatt ttgacccatt
     4381 aaataataac ggtatacgtt ttcttgttgg tgggttcacc ttattatttt tcattttatg
     4441 gagattccgc acatcgctgt ctcagctgat taaaactcca aaattgattg cgatttctct
     4501 atttgtcggg ttaatgatgt ccgcaaatat gtatttttgg ctaaaagggg tcaaaattac
     4561 aaatgctgtt accgtctcta tttttggggt attagctatg ccatttggtg tgattattgc
     4621 cgccatattc tttcaagatg aaagagaacg cattaaaagc aaagctttct ggttcggctc
     4681 aattttaacg atcatcggtt cactcggatt tatttggcac gggaaaacac tctcgattgg
     4741 cgaaggattt attcttggtt ctgtgttctt atttttgtcg atcattatcc gtaatataca
     4801 aaacctagtt gtaaaatttg ctcgagaact gaacgtatta accctaagtt gtgtaacatc
     4861 tttaactact tcactctcga gtctattact tagccaacaa gcaggtaaat ttaatgagct
     4921 ttttactgca ccaaactggc tactattctc tctgatggtt gctggtattt atgcgattat
     4981 gatcggaatg gtattaacct ttcacattat ccaaacccaa ggactgataa cctaccaagt
     5041 attggaacta ttaatgccgg tttcaacagc catcattgct tatattttac tggacgaacg
     5101 tatttccatt gttcaaatgg gctttgcttg tattgtgatt ttaggctcaa gtatcgcctt
     5161 aaagttagta aa
//
LOCUS       DANLYI010000166         5159 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.167, whole genome shotgun sequence.
ACCESSION   DANLYI010000166 DANLYI010000000
VERSION     DANLYI010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 5159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5159
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.167"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            48..728
                     /locus_tag="PXD81_002033"
     CDS             48..728
                     /locus_tag="PXD81_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007523984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00153 family protein"
                     /protein_id="HDL5538024.1"
                     /translation="MALNNILGLFAQSPLKPLQKHSNKVTECSELLESFFEATFEHDW
                     EKAAEVRGKIVDLERRADALKREIRLKLPRGLFMPVERGDLLELVTQLDKLANYSRDI
                     SGRIIGRKLIIPTEMQPLFKKFLSRSIDASRQVRKVLDEMDQLLETGFRGRELDFVNK
                     MILELDQIEDDTDQYQITLRHTLLELEKNLNPIDVMFLYKCIERISVLADQAQRIGSR
                     IELMLAKS"
     gene            740..2008
                     /locus_tag="PXD81_002034"
     CDS             740..2008
                     /locus_tag="PXD81_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005597096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HDL5538025.1"
                     /translation="MELLNQYGSILVIITAIVGFMMAFGIGANDVSNAMGTSVGSGTI
                     TIKQAILIAMVFEFAGAYLAGGEVADTIKSGIIDPAQFANSPDILVLGMMASLCAAGM
                     WLIIATKMGWPVSATHTIIGAVIGFALITIGSDAIQWDALGGIVGSWFVTPILAGIVA
                     YSIFINSQKLIFNRSNPFKQAKKYGPMYMGITIFILVIVTVSKGLKHVGLHLDTAETL
                     GISFALALLAFIIGYFYFRSKSFAMKARAEEKGFAGVEKIFSILMLITACAMAFAHGS
                     NDVANAIGPLAAVESIVRSGGMIEGKTTLAPWILPLGALGMMIGLAIMGKNVMATVGT
                     GITDLTPSRGFAAQFACAVTVVIASGTGLPISTTQTLVGAVLGVGFARGIAALNLGVI
                     RNIVISWVITLPVGAVLSIIIYYILSAIFY"
     gene            2063..2674
                     /locus_tag="PXD81_002035"
     CDS             2063..2674
                     /locus_tag="PXD81_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HDL5538026.1"
                     /translation="MKKLSSALLSCFLLGLSSPALTADTYITENLSTFMRKGAGDQFR
                     ISGAIQAGEKVTVLDRKERYSLIRDSRNREGWVLNSDLSDTASPKELIPQLKQQVQDL
                     TNRLSKIDTEWQQRTAEMQRRTQDSVQKSSDLVEENAQLKRELEILKNKNRDLETMHD
                     SEKREIVIQWFIYGGAVLGAGLLLGLLVPFLIPRRRRNNSGWA"
     gene            complement(2728..4026)
                     /gene="aroA"
                     /locus_tag="PXD81_002036"
     CDS             complement(2728..4026)
                     /gene="aroA"
                     /locus_tag="PXD81_002036"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HDL5538027.1"
                     /translation="MEKLTLTPISRVEGEINLPGSKSLSNRALLLAALATGTTQVTNL
                     LDSDDIRHMLNALKALGVKYELSDDKTVCVVEGIGGAFKVQNGLSLFLGNAGTAMRPL
                     AAALCLKGEEKSQIILTGEPRMKERPIKHLVDALRQVGAEVQYLENEGYPPLAISNSG
                     LQGGKVQIDGSISSQFLTALLMSAPLAEGDMEIEIIGDLVSKPYIDITLSMMNDFGIT
                     VENRDYKTFLVKGKQGYVAPQGNYLVEGDASSASYFLASGAIKGKVKVTGIGKKSIQG
                     DRLFADVLEKMGAKITWGEDFIQAEQSPLKGVDMDMNHIPDAAMTIATTALFAEGETV
                     IRNIYNWRVKETDRLTAMATELRKVGAEVEEGEDFIRIQPLALENFQHAEIETYNDHR
                     MAMCFSLIALSNTEVTILDPNCTAKTFPTYFRELEKLSVR"
     gene            complement(4072..>5159)
                     /locus_tag="PXD81_002037"
     CDS             complement(4072..>5159)
                     /locus_tag="PXD81_002037"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HDL5538028.1"
                     /translation="INIANEGVKSLSPYQAGKPIEELERELGIQNIIKLASNENPFGF
                     PESAKKAILNQLDDLTRYPDSNGFELKTTIAQKFGVQANQITLGNGSNDLLELFAHTF
                     AGEQDEIIYSQYAFIVYPLVTKAINAVAREILAKNWGHNLDAFLAAITEKTKLIFIAN
                     PNNPTGNFLTQAEIEGFLAKVPANVIVVLDEAYTEFTAPQERVDSFGLLAKYPNLIVS
                     RSLSKAYGLAGLRIGYAVSNPEIADLLNRVRQPFNCNSLALASAIAVMNDDEFVKKVA
                     ENNRLEMARYEAFCQANGLEYIPSKGNFITIDFKRPAAPIYEALLREGVIVRSIAGYG
                     MPNHLRISIGLPQENERFFTALLKVLA"
BASE COUNT         1467 a         1052 c         1052 g         1588 t
ORIGIN      
        1 tttacagtaa gaagtcattg tcaatattat cactatggag atattttatg gcattgaata
       61 acattttagg tttattcgcc cagtccccac ttaaaccgtt acaaaaacac tcaaataaag
      121 tgactgaatg tagcgagtta ttagaatctt tttttgaagc gacttttgaa catgattggg
      181 aaaaagcagc tgaagtgcgc ggtaaaattg ttgatttaga acgccgcgca gatgccctta
      241 aacgtgaaat ccgcttaaaa ctgcctcgtg gtttatttat gccagttgaa cgcggtgatt
      301 tattagaact agtcacacaa ttagataagt tagcaaacta ttcaagagat atttccggtc
      361 gcattattgg tcgtaaatta atcattccta ccgaaatgca accacttttc aaaaaattcc
      421 tttctcgtag tattgatgct agccgtcagg tacgcaaagt tttggatgaa atggatcagt
      481 tattagaaac tggttttaga gggcgagagc ttgatttcgt caataaaatg attcttgaac
      541 ttgaccaaat tgaagatgat accgaccaat atcaaattac attacgtcat acacttcttg
      601 agttagaaaa aaacctgaac cctattgatg tgatgttttt atacaaatgt attgaaagaa
      661 tcagcgttct agctgaccaa gcccagcgaa ttggttctcg tattgaactt atgctggcga
      721 agtcatagga gaaaattaaa tggaattact caatcaatat ggctcaatac tggtgattat
      781 tactgctatt gtagggttta tgatggcttt cggtattggt gctaatgatg tttcaaatgc
      841 catgggaaca tccgtcggct ctggaacaat tacgattaaa caagctattt taattgctat
      901 ggtatttgaa tttgctggtg cttatctggc aggcggtgaa gtcgcagata caattaaaag
      961 tggcattatt gatccggcac aattcgccaa tagccctgat atcctggtac ttggaatgat
     1021 ggcatctctc tgtgctgccg gaatgtggct aattattgca actaaaatgg gttggccggt
     1081 ttcagcaact cacacaatca ttggtgccgt tattggtttt gcattaatca ctattggttc
     1141 agatgcaatt caatgggatg cattaggtgg aattgttggg agttggtttg tgacccctat
     1201 tcttgcgggg atcgtagcat attcgatctt tattaactca caaaaactga tctttaaccg
     1261 ctcaaaccca ttcaaacaag cgaaaaagta tggcccgatg tacatgggaa ttactatttt
     1321 tattttagtt attgtaaccg tgtcaaaagg cttaaagcac gtaggattac atctagatac
     1381 agctgaaaca ttgggaattt cattcgcgct tgccttactt gcttttatca ttggatattt
     1441 ttatttccgc agtaaaagct ttgcaatgaa agctcgtgca gaagagaaag gcttcgctgg
     1501 cgttgagaaa atttttagta ttttaatgct tattacggcc tgtgctatgg cttttgctca
     1561 tggctctaat gatgttgcta atgctattgg gcctctagct gctgttgaat ctattgtaag
     1621 aagtggtgga atgattgaag gtaaaacgac tttagcccct tggattctcc cactaggcgc
     1681 cttaggtatg atgattggtt tagctattat gggcaagaat gtaatggcta cagtaggtac
     1741 tggtattacc gaccttactc caagccgtgg ctttgctgct caatttgcct gcgccgtcac
     1801 ggttgtcatt gcatcaggta ctggtttacc aatatcaaca acccaaacgc ttgtaggagc
     1861 agtattaggt gtgggattcg cccgtggtat tgctgcactt aacttaggcg ttattcgtaa
     1921 tattgttata tcatgggtaa ttaccttgcc tgtaggtgct gtgctttcaa ttattatcta
     1981 ttatatttta agcgctattt tctattaatt tttcaaaaag aatacaggca actgtattct
     2041 tcctagcttt agaggaatat atatgaagaa attatcatca gctcttctgt cttgtttttt
     2101 actcggccta tctagccctg ctttaacggc agacacttac attactgaaa atctgagtac
     2161 atttatgcgc aaaggtgctg gtgatcaatt ccgcatttca ggcgctattc aagcaggtga
     2221 aaaagtcaca gtattagatc gtaaagagcg ctacagtctg attcgagata gtcgtaaccg
     2281 tgaaggttgg gtacttaatt cagacttaag cgatactgct agtccaaaag agctgattcc
     2341 tcaattaaaa caacaggttc aagatttaac caatcgcctg agcaaaatcg ataccgaatg
     2401 gcaacaacgt accgcagaaa tgcaacgtag aacgcaagat tcagtgcaga aaagcagtga
     2461 tttggttgag gaaaatgcac aactaaaacg tgaactggaa atcctgaaaa acaaaaatcg
     2521 tgatttagaa acaatgcacg attccgagaa acgtgaaatc gtgattcagt ggttcatcta
     2581 tggtggtgct gtgttaggcg caggtttact acttgggtta ctcgtgcctt tcctgatccc
     2641 tcgccgcaga cgtaacaaca gcggttgggc ttagtctgcc acaataaaac gtaaaaagga
     2701 ttcagttttc tgaatccttt tttactttta tctgaccgat aatttttcca actccctaaa
     2761 gtaagtcggg aacgttttag cggtacaatt tggatctaag atcgtcactt ctgtattcga
     2821 taacgcaatt aatgaaaaac acattgccat acggtgatcg ttataggttt caatttcagc
     2881 gtgctggaag ttttctaacg caagcggttg aatccgaata aaatcttccc cttcttctac
     2941 ctctgccccg actttacgca attcggttgc cattgctgtc aagcggtcgg tttcttttac
     3001 ccgccagtta taaatattgc ggataactgt ttctccttcg gcaaataaag cggttgttgc
     3061 aatcgtcatt gccgcatcag gaatatgatt catatccata tctacgcctt ttagcgggga
     3121 ttgctcggct tgaataaaat cctctcccca agtgattttt gcccccattt tttccaacac
     3181 atcggcaaac aagcggtcgc cttggatcga ttttttacca atgcccgtta cttttacctt
     3241 gcctttaatc gcaccggagg ctaagaaata agaggcagaa gaggcatctc cctccaccaa
     3301 ataattacct tgtggagcaa catagccttg tttaccttta actaaaaagg ttttgtaatc
     3361 tcgattttca accgtaatac caaaatcgtt catcatcgaa agggtaatat caatataagg
     3421 ttttgatacc agatcaccga taatctcaat ttccatatcg ccttccgcta atggggcaga
     3481 catcagcaat gcggttagaa attggctgga aatcgagccg tcaatttgca cttttccgcc
     3541 ctgcaaaccg ctattgctaa ttgccaacgg tggatagcct tcattttcta aatactgtac
     3601 ctctgcccct acttggcgta aagcatcgac taagtgttta atcgggcgtt ctttcattct
     3661 tggttcaccg gtaagaatga tttgggattt ttcctcacct tttaaacaca atgctgctgc
     3721 aagtggtcgc attgccgtgc ctgcattgcc gagaaacagt gataagccgt tttgaacctt
     3781 aaaagctcca ccaatccctt caactacaca gacggtttta tcgtccgata gctcatattt
     3841 cacgcctaac gcttttaagg cattgagcat atgtcgaata tcatcactat ctaataaatt
     3901 ggtcacttga gtcgtaccgg tggctaaggc ggctaataat aaggctcggt tagacaggct
     3961 tttagaacca ggtaaattga tctcgccttc tactcgggaa atcggggtta aagttagttt
     4021 ttccattttt ctctttaaat ttgtaaaatt ttcataaaag gtaaccgctt gttaagctaa
     4081 cactttcaat aaggcagtaa aaaatctctc gttttcttgc ggtaaaccga tactaatgcg
     4141 taaatgattc ggcattccat aacctgctat cgagcgaaca atcacgcctt cacgcagtaa
     4201 tgcctcataa atcggagcgg caggacgttt gaaatcaata gtaataaaat tgcccttaga
     4261 agggatatat tccaagccgt ttgcttggca gaaagcctca tagcgagcca tttccaagcg
     4321 gttattttct gccacttttt tgacaaattc atcatcattc attaccgcaa tcgctgaagc
     4381 taaggcaaga ctattgcagt taaacggctg gcgaacacga tttaataaat ccgcaatttc
     4441 agggtttgaa accgcataac caatccgaag cccggctaag ccgtaagcct tagagagtga
     4501 acgtgacaca attaaattcg ggtattttgc caataagcca aacgaatcca cccgttcttg
     4561 aggcgcagta aactcggtat aagcttcgtc cagcaccacg attacatttg ccggcacttt
     4621 agctaaaaag ccctcaattt ccgcttgggt taaaaaatta ccggtcggat tattcgggtt
     4681 ggcaataaaa attaatttag ttttttcggt aattgcagcc aaaaaggcat ctaaattgtg
     4741 tccccaattt ttagccagaa tttcacgggc aacggcatta atcgctttcg tcaccaacgg
     4801 atacacaata aacgcatact gagaatagat aatttcatct tgctcaccgg cgaaggtgtg
     4861 ggcaaacagc tctaataaat cgtttgagcc attgcctaag gtgatttgat tggcttgtac
     4921 gccaaatttt tgggcaatag tggtttttaa ttcaaagccg tttgaatccg gatagcgggt
     4981 taaatcgtca agctgattca gaatcgcctt ttttgcactt tcaggaaaac caaatgggtt
     5041 ttcgttcgag gctaatttaa tgatattttg aatccctaat tcacgctcta gctcctcaat
     5101 cggctttcct gcttggtaag gcgaaaggga tttcacgccc tcatttgcga tgttgataa
//
LOCUS       DANLYI010000167         5149 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.168, whole genome shotgun sequence.
ACCESSION   DANLYI010000167 DANLYI010000000
VERSION     DANLYI010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 5149)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5149)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5149
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.168"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            222..2678
                     /gene="thrA"
                     /locus_tag="PXD81_002038"
     CDS             222..2678
                     /gene="thrA"
                     /locus_tag="PXD81_002038"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005603497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HDL5538029.1"
                     /translation="MRVLKFGGTSLANPERFMQAAEIIEKAHLADQAAGVLSAPAKIT
                     NHLVAIVDKAIAGESFQSNLSEATEIFHNIINGLYATNNHFAKEALLALVEAELNQIQ
                     QVAAEAAQTKSLSDKIAATIHCRGEKLSIAMMQAWFEAKGYEVTRIDPVEKLLAHGSY
                     LESSVDIAESTKRVDAGSIPKKNIVLMAGFTAGNEQGELVLLGRNGSDYSAACLAACL
                     KADVCEIWTDVDGVYTCDPRLVPDAVCLETMSYQEAMELSYFGAKVIHPRTIGPLVPL
                     NIPCLIKNTGNPDGKGTLIDGNVANDNLKVKGITNLDNVAMFNVSGSGMQGMVGMAAS
                     VFSTMSKAGISVILITQSSSEYSISFCVPAKSAEKALAALNTEFAQELSENLLDPIDI
                     IKDLSIVSVVGDGMRTAKGIAARFFSALAQANISIVAIAQGSSERSISAVVPLNKAIE
                     AVKATHKSLFNSKRVVDMFLVGVGGVGSELIEQVKQQKEFLAKKDIEIRVCALANSDK
                     MLLNENGLNLDNWKEDLEKAIQPSDFDVLLSFIKFHHVVNPVFVDCTSAQSVANLYAR
                     ALKEGFHVVTPNKKANTSGYDYYLEMRENARQSQHKFLYETNVGAGLPVIENLQNLLA
                     AGDEVEKFEGILSGSLSFIFGKLEEGLTLSQATLIAKEKGFTEPDPRDDLSGADVARK
                     LLILVRETGLPLEFEDIEIEGVLPKGFSEGMSRDEFLQALPSLDEEFSKRVEAAKAEG
                     KVLRYVGSITGDKCRVAIEAVDENHPLYKVKDGENALAFLTRYYNPIPLLLRGYGTGN
                     SVTAAGIFADILRTLQGGVN"
     gene            2678..3625
                     /gene="thrB"
                     /locus_tag="PXD81_002039"
     CDS             2678..3625
                     /gene="thrB"
                     /locus_tag="PXD81_002039"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HDL5538030.1"
                     /translation="MALRIYAPASSANISVGFDSLGAAISPIDGSLLGDVVQIEESEQ
                     QFELESAGYFVRKLPKEPQKNIVYQAYVLFSERLKLRNGSVKNLRLTLEKNMPIGSGL
                     GSSACSIVAALVALNKFHDEPFSKMELLEMMGELEGRISGSIHYDNVAPCYLGGLQLM
                     TQSLGNICQPLPFFDEWYWVLAYPGVEVSTAEARAILPKSYTRQDMIQQARYLGSFVH
                     ACHTHQDILAATMMKDLIAEPYREDLLPNFPAVRQGCKDLGALAVGISGSGPTMFAIA
                     PDLEHAQRLVSYLEKEYLQNNEGFIHICKVDNQGARELK"
     gene            complement(3740..5116)
                     /gene="cysS"
                     /locus_tag="PXD81_002040"
     CDS             complement(3740..5116)
                     /gene="cysS"
                     /locus_tag="PXD81_002040"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005597564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HDL5538031.1"
                     /translation="MLKIYNTLKREKEEFKPINPNQVGMYVCGVTVYDLCHFGHGRTF
                     VSFDVIARYLRYSGYNLRYVRNITDVDDKIIKRALENNETCDELVDRMIAEMHKDFDA
                     LNILRPDVEPRATKHIPEIIAMVEKLIHNGHAYVAADGDVMFDVESFQKYGALSRQNL
                     EQLQAGARVEIKSIKKNPMDFVLWKMSKAGEPSWESPWGNGRPGWHIECSAMNSKELG
                     EHFDIHGGGSDLMFPHHENEIAQSCCAHGGDYVNYWLHTGMLTIDKEKMSKSLGNFFS
                     IRHMLGLYDAESLRYFFLTAHYRSLLDYSIDNLDLARSALERLYTALRGCDVSGEAIG
                     EHYVEAFKTAMDDDFNTPGALAVLFEIARELNKLKTEDMAKANGLAVRLKELAGVLGL
                     LEQDPDAFLKGDANDDETAEIEALIKQRNEAKAAKNWAVADEVRDKLKAINIVLEDTP
                     NGTIWRKA"
BASE COUNT         1477 a         1119 c         1112 g         1441 t
ORIGIN      
        1 caaaggcatt attttcacgc attcttgaac cattattcac tctcttgacc gagattccgc
       61 caatgaattg ttttaatatg atgacaacga ttattaccac cattatgtca ccaacatagt
      121 gcgggtttta attggctgaa ataatgaaag aataaaccga aaacccgcta caagcgggtt
      181 tttttgtata aaaatttaca aataatttat caggagtaac tatgcgagtt cttaaatttg
      241 gaggcacttc tcttgccaac cctgaacgct ttatgcaagc ggcagagatt atcgagaaag
      301 ctcatttggc agatcaagct gccggcgtat tatccgcccc agccaaaatt accaatcatc
      361 ttgtcgctat tgtagataaa gcgattgccg gtgaatcatt tcaatctaac ctttctgaag
      421 ctaccgagat tttccacaac atcattaacg gcttatatgc tacaaataat cactttgcta
      481 aagaagcact gttagcctta gtggaagcgg aattaaacca aattcagcaa gtggctgcag
      541 aagcagctca aactaaatcg ttgtcagata aaattgctgc cactattcac tgccgtggtg
      601 aaaaactctc tattgcgatg atgcaagcgt ggttcgaggc aaaaggctat gaagtcactc
      661 gtatcgaccc tgttgaaaaa ttattggctc acggcagcta tttggaatct tcggtcgata
      721 ttgccgaatc caccaagcga gttgatgcag gttcaattcc aaagaaaaat atcgtgctaa
      781 tggctggttt taccgcaggt aatgagcaag gcgaactggt gttattaggg cgtaacggtt
      841 cagattattc tgccgcttgt ttggcagcgt gtttaaaagc cgatgtttgt gaaatctgga
      901 cggacgtaga tggcgtttac acctgcgacc ctcgtttggt gcctgatgca gtatgcttag
      961 aaacaatgag ctatcaagag gcaatggagc tttcctactt tggggcgaaa gtgatccacc
     1021 ctcgcaccat cgggccatta gtgccgctca atattccttg tttaatcaaa aataccggca
     1081 atccggacgg aaaaggcaca ttaattgatg gcaatgtggc aaacgataat ctcaaagtaa
     1141 aagggattac caaccttgat aacgtggcga tgttcaacgt ttcaggttca ggaatgcagg
     1201 gaatggtggg catggctgcc agtgtgttct cgacgatgtc aaaagccggt atttcggtga
     1261 ttttaattac ccaatcatca tcagaatata gcatcagctt ctgcgtgccg gcaaaatcgg
     1321 cagaaaaagc gttagcggct ctaaatactg aatttgcaca agagttatct gaaaacttac
     1381 ttgacccgat tgatattatt aaagatttat ccattgtttc ggtagtgggt gatggtatgc
     1441 gtaccgccaa aggaattgcg gctcgcttct tctctgccct agcccaagcc aacatcagta
     1501 ttgtggcaat tgcacagggt tcatctgagc gttccatttc tgctgtcgtg ccgttaaaca
     1561 aagcgattga agcagtaaaa gcaactcaca aatcgctatt taacagtaaa cgtgtggtgg
     1621 atatgttttt agtgggtgta ggcggtgtgg gtagcgaact catcgagcaa gtcaaacaac
     1681 aaaaggagtt tttggcaaag aaggatattg agatccgtgt gtgtgcttta gctaattcag
     1741 ataaaatgtt actcaacgaa aacggcttaa atttagacaa ctggaaagaa gatttagaaa
     1801 aagcaattca gccgtcagac tttgatgtat tgctttcttt catcaaattc caccacgtgg
     1861 taaacccagt atttgtagat tgcacttcgg cacaatcggt ggcgaacctg tacgcacgag
     1921 ccttaaaaga gggcttccac gtggtaacac caaacaaaaa agccaataca tcaggttacg
     1981 attactactt agaaatgcgt gaaaacgccc gccaaagcca gcacaaattc ctctacgaaa
     2041 ctaatgtagg ggcaggttta ccggtaattg aaaacttaca aaacctatta gctgccggtg
     2101 acgaagtaga gaaatttgaa gggattttat ccggctcgct ctctttcatt ttcggcaaat
     2161 tagaagaagg tttaaccctt tcgcaagcaa ctttaatcgc caaagaaaaa ggctttaccg
     2221 agccagatcc acgagatgat ttatccggtg cagatgttgc tcgtaaactc ttaattcttg
     2281 ttcgtgaaac cggcttaccg cttgaatttg aagatattga aattgaaggc gtattgccaa
     2341 aaggcttctc ggaaggaatg agtcgtgatg agtttttaca agctctaccg tctttagacg
     2401 aagaattcag caagcgtgta gaagctgcca aggctgaagg caaagtgttg cgttatgtgg
     2461 gctcgatcac aggtgataaa tgccgagttg ccattgaagc cgtggacgaa aaccacccac
     2521 tttacaaagt taaagacggc gaaaatgccc ttgcttttct cacacgttac tacaacccta
     2581 ttccattatt attgcgtggg tatggtacgg gcaatagcgt aaccgctgcc ggtatctttg
     2641 cagatattct gcgtacttta caaggaggag tgaactaatg gcgttacgaa tttatgcccc
     2701 agcttcaagt gcaaatatta gtgtgggttt tgactcatta ggcgctgcaa tctccccgat
     2761 tgacggctct ttgctcggcg atgtagttca aattgaagag agtgaacaac aatttgagct
     2821 agaaagtgca ggttattttg tacgtaaatt gcccaaagaa ccacaaaaaa acattgtcta
     2881 tcaagcctat gtgctattta gcgaacgctt gaaattacgt aacggcagtg taaaaaattt
     2941 acgcttaaca cttgaaaaaa atatgccgat tggctcaggg ctaggctcaa gtgcctgctc
     3001 gattgttgca gcattagtgg cactcaataa attccacgat gaaccgttct ccaaaatgga
     3061 attactggaa atgatgggcg aattggaagg cagaatttca ggctcaatcc actacgacaa
     3121 cgttgcccct tgctatttag gtggcctgca attaatgaca caatcgctcg gcaatatttg
     3181 ccaacctctg ccattctttg atgaatggta ttgggtgctt gcctacccgg gcgtggaagt
     3241 atcaaccgcc gaagcccgtg ctattttgcc gaaaagctat actcgtcaag atatgattca
     3301 gcaagcccgc tacttaggat ctttcgtcca tgcttgccat acacatcaag atatcttggc
     3361 tgctacaatg atgaaagacc taattgctga gccttaccgt gaagatttac tgccgaactt
     3421 tccggctgtt cgtcaaggct gtaaagactt aggtgcgtta gcggttggta tttcaggttc
     3481 cggtccgaca atgtttgcga tagcccctga tctcgaacac gctcaacgtt tagtgagcta
     3541 tttagagaaa gaatatctgc aaaacaacga aggctttatt catatctgta aagtcgataa
     3601 tcaaggagca agagagctga aatagccctc tcgaacaacc atatcactaa ataacttaaa
     3661 ggactgactc caataccact tggagcagcc ctttttgatt aattttttga aaagtctatc
     3721 acttataaac aatcgaaggc tatgccttac gccaaattgt gccgtttggg gtgtcttcga
     3781 ggacaatatt tatcgctttg agcttatctc gcacttcatc ggcaaccgcc cagtttttag
     3841 ccgctttggc ttcgttgcgt tgtttgatta aggcttcgat ttctgccgtt tcatcgtcat
     3901 tcgcatcacc ttttaaaaac gcatcggggt cttgttccag taagcctagc acgccggcta
     3961 actcttttaa acgaacggct aaaccgttgg cttttgccat atcttcggtt ttgagtttgt
     4021 tcaactcacg ggcaatttca aataacaccg ctaatgcccc cggggtgttg aaatcatcgt
     4081 ccattgcggt tttaaaggct tctacatagt gttctccgat ggcttcgccc gacacatcac
     4141 aaccacgcag agcggtatat aaacgctcaa gagccgaacg ggctaaatca aggttgtcaa
     4201 tgctgtaatc taataacgaa cggtaatgag cggttaagaa gaaataacgt aacgattcgg
     4261 catcgtataa gcctaacata tggcgaatgc tgaagaaatt gcccaaagat ttcgacattt
     4321 tttctttatc aatggtaagc attccagtgt gtaaccaata attgacataa tcgccaccgt
     4381 gagcacagca agattgtgca atttcgtttt cgtgatgcgg gaacattaaa tccgagccac
     4441 cgccgtgaat atcaaaatgt tcgcccaact ctttgctatt cattgccgaa cattcaatat
     4501 gccagcccgg tctgccattg ccccacgggc tttcccagct tggctcaccg gctttcgaca
     4561 ttttccacaa gacaaaatcc atcgggtttt tcttgatgga tttaatttca acccttgcac
     4621 cggcttgtag ttgctctaaa ttttggcggg ataatgcacc atatttctgg aacgattcca
     4681 catcaaacat cacatcgcca tcggctgcga cataagcgtg tccgttgtga atcagttttt
     4741 ctaccatcgc aataatttca ggaatatgct tggtggctcg tggctcaacg tccgggcgaa
     4801 gaatgtttaa cgcatcaaaa tctttgtgca tttcagcaat cattctatcc actaattcat
     4861 cgcaagtttc gttattttct aacgcacgtt tgatgatttt gtcgtccaca tcggtaatgt
     4921 tacgcacata gcgtaaattg tagcctgaat aacgtaaata acgggcaatc acatcgaaag
     4981 agacaaaagt gcgaccgtgc ccaaaatgac aaagatcgta aaccgtgacg ccacagacgt
     5041 acattcccac ctgatttggg ttaatcggtt taaattcttc tttttcacgt tttaaggtgt
     5101 tataaatttt gagcataaag tcctcttgtg taacattcag taacaaatg
//
LOCUS       DANLYI010000168         5146 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.169, whole genome shotgun sequence.
ACCESSION   DANLYI010000168 DANLYI010000000
VERSION     DANLYI010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 5146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5146
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.169"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            <1..513
                     /locus_tag="PXD81_002041"
     CDS             <1..513
                     /locus_tag="PXD81_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase family
                     protein"
                     /protein_id="HDL5538032.1"
                     /translation="ANTLWQEKKIRLLLNPQGSMREISTQELSELLSMIDKRYVQHIS
                     CLLTNTTNSAEYLSKIKAPFEISLAPNSDILGYCALVNSADIVIAVDGGGVHISCALG
                     KKLLSFYANHPANIARWYPKPKKDVETFMVIADKWTEDNNDTRGFSLVAPAKWLNHQF
                     AKLIEKYTAC"
     gene            complement(568..780)
                     /gene="rpmE"
                     /locus_tag="PXD81_002042"
     CDS             complement(568..780)
                     /gene="rpmE"
                     /locus_tag="PXD81_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005625828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HDL5538033.1"
                     /translation="MKQGIHPEYKEITATCSCGNVVKTRSTVGKDLNLDVCGNCHPFY
                     TGKQRVVDTGGRVERFNKRFSIPSSK"
     gene            1143..3149
                     /gene="tkt"
                     /locus_tag="PXD81_002043"
     CDS             1143..3149
                     /gene="tkt"
                     /locus_tag="PXD81_002043"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HDL5538034.1"
                     /translation="MTERKVLANAIRFLSMDAVQKANSGHPGAPMGMADIAEVLWRDF
                     LKHNPTNPKWADRDRFVLSNGHGSMLIYSLLHLTGYDLSIEDLKQFRQLHSKTPGHPE
                     YGYAPGVETTTGPLGQGITNAVGIAIAEKTLAAQFNREGHKIVDHYTYAFLGDGCLME
                     GISHEACSLAGTLGLGKLIAFYDDNNISIDGHVDGWFTDDTAQRFESYGWQVIRNVDG
                     HNPEQIKFAIENAQAETERPTLIICKTIIGYGSPNKSNSHDCHGAPLGDAEIQAAREF
                     LGWEHAPFVIPAEIYTEWDAKAKGTLAEKDWNAKFAAYEAAYPELAAEFKRRYAGELP
                     ANWEAESKAFIEKLQANPATIASRKASQNAIEAYAPILPEFLGGSADLASSNLTLWSG
                     SKPIRAEHNVDGNYINYGVREFGMSAIMNGIALHGGFIPYGATFLMFYEYAHNAVRMA
                     ALMKQRALFVYTHDSIGLGEDGPTHQPVEQTASLRQIPNLETWRPADQVESAVAWKAA
                     VERKDGPTALIFTRQNLAQQQRTAEQLANISRGGYILRECCEKGSCPDLILIATGSEV
                     DLAMKAADVLDAEGVKVRVVSMPSTNVFDKQDEAYRESVLPRSVTKRVAIEAQLADFW
                     YKYVGFDGRIVGMNSFGESAPADQLFKLFGFTVENVVAKAKEIL"
     gene            complement(3275..4345)
                     /locus_tag="PXD81_002044"
     CDS             complement(3275..4345)
                     /locus_tag="PXD81_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HDL5538035.1"
                     /translation="MNEIIFSLEPQDNARLQSLCGAFDEHLALIEKSFNLIIARTGFN
                     FTIQSEDDNHYSATLVQNSVKLIKQLYNETAPIKGKIKELDLEDIHLAIQESRMLLQN
                     QWQAGSQGEIAIKTKRGVIKPRSEHQQAYLRNILSHDISFGIGPAGTGKTFLAVAAAV
                     ESLERQEIRRILLTRPAVEAGEKLGFLPGDLGQKIEPYLRPLYDALFEMLGFEKAQKL
                     MERGVIEIAPLAYMRGRTLNDAFIILDESQNTTTEQMKMFLTRIGFNSKAVITGDITQ
                     VDLPRSQKSGLKHAMEVLKEVPELSFNFFDSKDIVRHPVVAKIVQAYDAWEAVDEERK
                     EQRKLEKLALEQEKILAQAEML"
     gene            complement(4360..5085)
                     /gene="fadR"
                     /locus_tag="PXD81_002045"
     CDS             complement(4360..5085)
                     /gene="fadR"
                     /locus_tag="PXD81_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005614584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HDL5538036.1"
                     /translation="MGHSDILKAQSPAALAEEYIVKSIWNNHFPAGSDLPAERELAER
                     IGVTRTTLREVLQRLAREGWLSIQHGKPTRVNDIWETSGPNIIGTIIKLDKSYLPVVI
                     ANVVSLRTRMAEAYIPEAIYLAPEESAKLFDGLDELENTAAAYAEFDYKLFRQFTFLA
                     NKPVYALILNSFKSMYHQVAKIFFADENNRQLTLSFYRSLLCTCLAKDSEKAAACMAK
                     NRQSSGEIWQKLSANIPESFGQL"
BASE COUNT         1543 a         1077 c         1007 g         1519 t
ORIGIN      
        1 gcaaatacgc tatggcaaga gaagaaaatt cgattactat tgaatcctca aggtagtatg
       61 cgtgaaattt ctactcaaga attatcagag ctattatcta tgattgataa aagatatgtt
      121 caacatatta gttgtttgct gaccaataca accaatagtg ccgaatattt gtcgaaaatt
      181 aaagccccat ttgaaataag cctagcacct aatagtgata ttttaggtta ttgtgcatta
      241 gtaaactctg ctgatattgt aattgcagtt gatggaggtg gtgtccatat ttcttgtgca
      301 ttaggcaaga aattgctctc attttatgcc aatcatccag ctaatattgc acgctggtat
      361 ccaaaaccta agaaagatgt tgaaactttt atggtgatcg cagataaatg gaccgaggat
      421 aataatgata ctcgaggttt ttctctggta gctcctgcaa aatggttaaa tcaccaattt
      481 gcaaaattga tagaaaaata caccgcttgt taattcgata tataagaaaa acaaaacccc
      541 gcattagcgg ggtttgcaat aatcaaatta ttttgagctt gggatgctga aacgtttgtt
      601 aaagcgttca acacgaccac cggtgtcaac aacacgttgt ttaccagtat agaacgggtg
      661 gcagttgcca catacgtcta agtttaaatc tttacccact gttgagcgag ttttaactac
      721 attaccgcaa gagcaagttg ccgtaatttc tttatattct gggtgaatac cttgtttcat
      781 tggggaacct cataagaagc catatcgccg cctatcttgt tagaatctct accgctgata
      841 ggtaccatat gtaattaata aaaatgttga gcaaatgctc aacgccaaaa agagtgcgta
      901 ttttagataa atttgttgcc tagatcaact actaaacatt cgctttatca agaaaatatt
      961 gtattaggaa aaaaccgtct attaaaatca aatccaatca aaacaaatca caatttaatc
     1021 attttttgtg atacagatca catttctcat ttccattttt gagtttttct agcaaaatag
     1081 tgtagaatcc aattcgatat cctttccatt ttatataaca ttctcgatta ggagtaacac
     1141 aaatgacaga acgtaaagtg ctagcgaatg caattcgttt tttaagtatg gatgctgtac
     1201 aaaaagctaa ctcaggccac ccaggcgcac caatgggtat ggctgatatt gcagaagttt
     1261 tatggcgtga ttttttaaaa cacaatccaa ctaatcctaa atgggcagat cgtgaccgtt
     1321 ttgttctgtc taacggacac ggttcaatgt taatctatag cttacttcat ttaaccggct
     1381 atgatctctc tattgaagat ttaaaacagt tccgtcagtt acattctaaa acacctggtc
     1441 accctgaata tggttatgca ccgggtgttg aaaccaccac aggcccgtta ggtcaaggta
     1501 ttaccaatgc ggtcggtata gcgattgcgg aaaaaaccct tgcagcacaa tttaaccgtg
     1561 aaggtcataa aattgtcgat cactacactt acgctttctt aggcgatggc tgtttaatgg
     1621 aaggtatctc acacgaagct tgctctctcg ccggtacatt aggcttaggc aaactgattg
     1681 ctttctacga tgataacaat atctctatcg acggccacgt tgatggttgg ttcactgatg
     1741 ataccgcaca acgctttgag tcttatggct ggcaagttat ccgtaatgtc gatggccata
     1801 acccagaaca aatcaaattt gcgattgaaa atgcacaagc cgaaaccgag cgtccaactt
     1861 taattatttg taaaaccatt atcggctatg gttcaccaaa taaatcaaac tctcacgatt
     1921 gccacggtgc gccattgggt gatgctgaaa tccaagccgc tcgtgaattt ttaggttggg
     1981 aacatgcccc atttgtgatt ccagctgaaa tttatacaga gtgggatgca aaagcaaaag
     2041 gtacattagc agaaaaagat tggaacgcta aatttgctgc ttatgaagcg gcataccctg
     2101 aactagccgc tgaatttaaa cgccgttacg caggcgaatt accggctaac tgggaagctg
     2161 aaagcaaagc ttttattgaa aaattacaag caaatccggc aaccattgca agccgtaaag
     2221 catcacaaaa tgcaatcgaa gcttacgcgc caatcttacc tgaattcctc ggcggctcag
     2281 ctgacttagc aagctctaac ttaaccttat ggtctggctc aaaaccgatt cgtgcggaac
     2341 acaatgtaga tggtaactac atcaactacg gcgtgcgtga attcggtatg tctgcgatta
     2401 tgaacgggat tgccctacac ggtggtttca ttccttacgg tgcaaccttc ttaatgttct
     2461 acgaatatgc acacaatgcg gtgcgtatgg cagccttaat gaagcaacgt gccttatttg
     2521 tttatacaca cgactccatc ggtttaggtg aagacggccc gacccaccaa ccggtagaac
     2581 aaactgcttc attacgccaa attccaaact tagaaacctg gcgtccggca gaccaagtag
     2641 aatctgctgt tgcttggaaa gcggcggtag aacgtaaaga cggcccgaca gcactaatct
     2701 ttacccgcca aaatctggct caacaacaac gtactgcgga gcaattagcg aacatctctc
     2761 gtggtggcta tatcttgcgt gaatgttgcg aaaaaggcag ttgcccggat ttaatcttaa
     2821 ttgcaaccgg ttctgaagta gatttagcaa tgaaagccgc tgatgtgtta gatgcagaag
     2881 gcgtgaaagt gcgtgtagtt tcaatgccaa gcaccaacgt gtttgacaaa caagatgaag
     2941 cctatcgtga atcggtatta ccacgttcag tcactaaacg tgtggcgatt gaagctcaat
     3001 tagctgactt ctggtataaa tatgtcggct ttgatggtcg aattgtaggt atgaatagct
     3061 ttggtgaatc cgcaccagca gatcaattat tcaaactctt cggctttacc gttgaaaacg
     3121 tggttgctaa agcaaaagaa atcttataat gaaacaataa gataaagaaa agcgggaatt
     3181 aacttcccgc ttttttgttg aacaactgtt atgataagcg gtgaaatttc agcgattttt
     3241 tgcaaaaaat tagcgaaata tgaccgcttg taagctataa catttcagct tgagcaagaa
     3301 ttttttcttg ctctaaagct aatttttcca gttttcgctg ttctttacgc tcttcatcaa
     3361 ccgcttccca cgcatcatag gcttgcacaa ttttggcaac cacagggtgg cggacaatat
     3421 ctttgctgtc aaagaaatta aagctgagtt ccggcacttc ttttagcact tccattgcgt
     3481 gttttaagcc tgatttttgg ctacggggca aatccacttg ggtaatatcg ccggtaatca
     3541 ccgctttgga attaaagcca atacgggtta agaacatttt catttgctcg gtagtcgtgt
     3601 tttggctttc atctaaaatg atgaacgcat cgtttaaagt acgccctcgc atataagcaa
     3661 gtggggcaat ttcaatcaca ccacgttcca tcaacttttg agctttttca aagccaagca
     3721 tttcaaacag ggcatcatat aacgggcgta agtaaggctc aattttttgc cctaaatcgc
     3781 cgggtaaaaa accgagcttt tcaccggctt ccaccgccgg acgggtaagt aaaatacggc
     3841 ggatttcttg gcgttcaagc gattccaccg ctgccgctac cgctaaaaag gttttgcctg
     3901 tgccagcagg cccgatacca aaactgatgt cgtggcttaa tatatttcgt aaataggctt
     3961 gttgatgttc gctacgaggt ttaatcaccc ctcgcttggt tttaatcgct atttcgcctt
     4021 gcgagccggc ttgccattgg ttttgtagta gcattcggct ttcctgaatc gccaaatgta
     4081 tatcttctaa gtccaattcc ttaattttgc ctttaatcgg ggcagtttca ttatacagtt
     4141 gcttaatcag cttcacacta ttttgaacca aggtagcaga atagtgattg tcatcttccg
     4201 attgaatcgt aaagttgaag cctgttcgag caataatgag attaaaactt ttttcaatta
     4261 aggctaaatg ctcatcaaat gcaccacaaa gcgattgcag acgagcatta tcttgcggtt
     4321 ctaagctaaa aataatttcg ttcaaaattc taccttctgt taaagctgac caaagctttc
     4381 aggaatatta gcggataatt tttgccaaat ttcaccacta gattggcgat ttttagccat
     4441 acaagcggct gctttttcac tatcttttgc aagacaagta cataataatg aacggtaaaa
     4501 gcttaacgtt aattgacgat tattttcatc agcaaagaaa atttttgcga cttgatgata
     4561 cattgattta aagctattta ggatcaacgc atacacaggc ttattggcta aaaaagtaaa
     4621 ttgtctgaat aacttataat caaattcagc ataagcagct gctgtatttt ctaattcatc
     4681 tagtccatca aagagtttag cactttcttc aggagcaagg taaatcgctt ctggaatata
     4741 agcctccgcc atacgagtac gtaatgacac cacattggca atcaccactg gaagatagct
     4801 tttatctagc ttaataatag tgccaataat attaggcccc gaagtttccc aaatatcatt
     4861 cactcttgtt ggcttgccgt gctgaatact taaccaacct tctcgcgcta gacgttgtag
     4921 tacttcacgt aaagttgttc ttgttacccc aatgcgctct gctaactctc gctcggcagg
     4981 aagatcagag ccggcaggga aatgattatt ccaaatactc tttacaatat attcttccgc
     5041 taacgctgcc gggctttgcg cttttaaaat atcactgtga cccattaatg ccccccaagc
     5101 aaaaaatatg cttatctaaa atctaactat tctttatttc taatca
//
LOCUS       DANLYI010000169         5095 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.170, whole genome shotgun sequence.
ACCESSION   DANLYI010000169 DANLYI010000000
VERSION     DANLYI010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 5095)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5095)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5095
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.170"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            complement(195..1094)
                     /gene="cdd"
                     /locus_tag="PXD81_002046"
     CDS             complement(195..1094)
                     /gene="cdd"
                     /locus_tag="PXD81_002046"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HDL5538037.1"
                     /translation="MFRTDITERFEQLKAAKNCEVTQAIIEQLIKQDFHGQLSYEVVD
                     ELCEKFKRSRVELALYCIAIAACYAVTPVSDFNVGAVAIGKNGDFYFGANQEFSGECM
                     QQSVHAEQSAISHAFLAGETLITDVVVNYTPCGHCRQFMNELNSAASLKVHLPHSQNN
                     LLHSYLPDSFGPKDLGIEKVLFDEQPQQFEVKGDHLQQAAIFAANNAYAPYSNAFSGV
                     ALQVGERIVCGKYTENAAFNPTFLPLQSALNYRLFLGLADIPVTRVVLAEAKGSLSSY
                     AMTQALAKSLFGLEIEYLSLQAV"
     gene            complement(1108..2010)
                     /locus_tag="PXD81_002047"
     CDS             complement(1108..2010)
                     /locus_tag="PXD81_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDL5538038.1"
                     /translation="MISSSLKLGYFSLIVATLFWGGNFVLGKVLSGAVQPITLTYLRW
                     LPAFLILVLLFFRPTWRVMHLLKPRLGVMWVLGLLGVILFPATLYEGLKTTTALNASL
                     YLAISPVLVLFLNRLVFKEKISPIILSGAIISLLGVLWLLMQGKLTRLFSLAINQGDL
                     WAIASTISWAVYCCVIRLRPQGISNTVFLTVLVGLAVLTLTPFFIYEYVAESAKNLAN
                     LTALQWSGIGYLVIGPSILSYAFWNFGIAIVGSAKGAVMTNFTPLFAAMFSIILLNES
                     VHTYHIISAILITAGILICSYKKH"
     gene            complement(2007..2363)
                     /gene="folB"
                     /locus_tag="PXD81_002048"
     CDS             complement(2007..2363)
                     /gene="folB"
                     /locus_tag="PXD81_002048"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HDL5538039.1"
                     /translation="MSDKVFIRELTAFASIGAYNWEHTIKQRLVFNIDMAWDFSKAAE
                     TDDVQYCLNYAEVSQKVLDFVESQPFKLVETVAYQLAELLQKEYQLSWIRIELHKPKA
                     VAQASSVGVIVERGHQ"
     gene            2433..3038
                     /gene="plsY"
                     /locus_tag="PXD81_002049"
     CDS             2433..3038
                     /gene="plsY"
                     /locus_tag="PXD81_002049"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HDL5538040.1"
                     /translation="MNLIPYLLILTAYLLGSISSAVIFCKLAGLPDPREVGSHNAGAT
                     NVLRIGGKSAALGVLLFDILKGTLPVAMGIYFELSPSQLGFIALAVCLGHIFPIFFQF
                     RGGKGVATAFGAISALSFIVAGGAIGTWLLVFLISGYSSLSAVFTALVVPFYIWWFEP
                     QFTFPVALVCCLLVYRHHDNIQRLWRGQEDKLWDKFKKNRA"
     gene            complement(3112..5019)
                     /gene="parE"
                     /locus_tag="PXD81_002050"
     CDS             complement(3112..5019)
                     /gene="parE"
                     /locus_tag="PXD81_002050"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009875262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HDL5538041.1"
                     /translation="MSEKRYGADEITVLKDLEPVQLRPGMYTDTTRPNHLGQEVIDNS
                     VDEALSGFADKIDVILHADNSLEVVDNGRGMPVDIHSTEKISGVELILTKLHAGGKFS
                     NKNYTFSGGLHGVGISVVNALSERVEIQIKRGGEVYSIAFANGVKVEDLTVIGTCPKK
                     QTGTTVRFYPNPKYFDSPRFSVSRLRHLLRAKAVLCPKLTINFIDKINNTEESWYYED
                     GLSDYLSEALNGYETVPNPPFIGDVTAETEAVSWALTWLPEGGELVAESYVNLIPTAQ
                     GGTHVNGLRNGLLKAMVEFCEIHNLLPKGVKLTADDVWNRCAYVLSLKIQEPQFAGQT
                     KERLSSRQASSYVDSTLKDAFSLWLNQNVQTGKLIAEMAISSAQSRMRAAKKVVRKKL
                     VSGPALPGKLADCTAQDLSRTELFLVEGDSAGGSAKQARDKEYQAILPLRGKILNTWE
                     VSSDQVLASQEVHDIAVALGIDPDSDNLEELRYGKVCILADADSDGLHIATLLCALFL
                     RHFPKLVQNGHVYVAMPPLYRIDIGKDEVHYALDEAEKEAILARLAKKKGKPNVQRFK
                     GLGEMNPSQLRETTMEPSTRRLVQLTFDDTVEEGELNTFEIMDMLLGKKRAEDRKQWL
                     QNRGDEADLAV"
BASE COUNT         1493 a         1209 c          940 g         1453 t
ORIGIN      
        1 tttttactcg gttcaataag aggagtattt tccataaaaa cctcacaaaa tatagataat
       61 cagtggtatc actatatata aaaaaacgat ataggcaaat ataattgttg caaaagtgtg
      121 aagctcatca aaaaatttaa cactaaaaaa taaattgctt aaagatacag cacctttgca
      181 aaaataaatc aaaattacac cgcttgtagc gaaaggtatt caatctctaa cccaaatagt
      241 gattttgcca atgcttgagt cattgcataa ctactgagag agcctttcgc ctcagccagc
      301 acaactcgag taacgggaat atcggcaagc cctaaaaata atcgatagtt caacgcgctt
      361 tgcaacggta aaaaagtagg attaaaagct gcattctcag tatatttacc acaaacaata
      421 cgctcaccta cttgcagtgc aaccccacta aatgcgttgc tgtaaggggc ataagcatta
      481 ttggcagcaa aaattgccgc ttgttgtaaa tgatcgccct taacctcaaa ttgttgcggt
      541 tgttcatcaa ataagacttt ctcaatccct aaatctttcg gtccaaaact atccggcaaa
      601 tagctatgga gcaaattatt ttggctatgc ggtaaatgta cttttaagct tgccgcactg
      661 tttaattcat tcataaattg gcggcaatgc ccacaagggg tatagttcac aaccacatct
      721 gttatcaaag tttcccctgc taaaaatgcg tgcgagattg cactttgctc tgcgtgaacg
      781 gattgttgca tacattcccc ggaaaactct tggtttgccc caaaatagaa atctccgttt
      841 ttgccaatcg caaccgcccc tacattaaaa tcggaaactg gtgtaaccgc ataacaggca
      901 gcaatggcaa tgcaatataa agccaactca acacgagagc gtttaaattt ttcacagagt
      961 tcatcaacca cttcgtaaga gagctgcccg tgaaaatctt gcttgataag ttgctcaata
     1021 atagcttgtg taacttcaca gtttttcgct gcttttaatt gttcaaaacg ctctgtaatg
     1081 tctgttcgaa acatactgta cctccgttta atgtttttta tagctgcaaa tcagtatccc
     1141 tgccgtaatc agtatggcac taataatatg gtaagtatga actgattcat ttaataaaat
     1201 aatactgaac attgctgcaa agagcggcgt aaaattagtc attaccgctc ctttagcaga
     1261 accgactatt gcaatgccga aattccaaaa cgcataagag agaatagaag ggccgataac
     1321 caaatagcct atcccactcc attgtaaagc ggtcaaattt gctaaatttt ttgcagattc
     1381 tgctacatat tcatagataa aaaatggcgt taacgttaga actgccagcc ccactaatac
     1441 ggtcaaaaac accgtattgc ttataccttg tggacgtagc cgaatcacac aacaataaac
     1501 cgcccagctt atagtagaag caatcgccca taaatcacct tgattaattg ccaagctgaa
     1561 taagcgagtt agcttccctt gcatcaatag ccacagcact cccagcaaac tgattattgc
     1621 cccacttaga ataatcgggc tgattttctc cttaaaaacg agccgattta aaaatagcac
     1681 caatacaggc gaaatagcta aatagagact cgcattcaat gctgttgtcg tttttaaacc
     1741 ttcatataag gtagccggga ataaaattac cccaagcaga cctaataccc acatcacacc
     1801 taaacgaggt tttaataagt gcattacccg ccaagttggg cgaaaaaaga gcaaaactaa
     1861 aattaaaaaa gccggcaacc aacgcaaata ggttaaggta ataggctgaa ctgccccact
     1921 taacactttg cctaatacaa aattcccacc ccaaaaaaga gtagcaacga ttaaactgaa
     1981 ataccccaat ttcagtgatg atgagatcat tgatgacctc gttctacaat cacacctaca
     2041 ctgcttgctt gggcaaccgc tttcggctta tgcagttcaa tccgaatcca agaaagttga
     2101 tactcttttt gtaatagttc cgccagttga taggcaacag tttcaactaa tttgaaaggt
     2161 tgagattcaa caaaatccag cactttttgc gatacttccg cataattcaa gcaatattgt
     2221 acatcatcgg tttctgccgc tttggaaaaa tcccacgcca tatcaatgtt aaaaaccaag
     2281 cgttgcttaa tcgtatgctc ccaattgtaa gccccaatgg aagcaaaagc ggttagttct
     2341 cgaataaaaa ccttatcact catttttcgt cctgttttat tttctgaatt tctgtaaaat
     2401 atagcacaag ataaatttaa tcaggaaaat ttatgaattt aattccctat ttattgatct
     2461 taaccgccta tttgctcggc tcgatttcca gtgcggtcat tttttgtaag ctggcaggct
     2521 tgcctgaccc aagagaagtc ggctcacaca atgccggtgc aacgaacgta ctacgaattg
     2581 gcggtaaaag tgccgcatta ggtgtgcttt tattcgatat tttaaaaggc acattaccgg
     2641 tggcgatggg gatttatttt gaactctctc cctcacaact cggtttcatc gccttagcgg
     2701 tttgtcttgg gcatattttc ccgattttct tccaattccg tggcggaaaa ggcgttgcca
     2761 ccgcatttgg tgcaatctcc gcattaagct ttattgttgc aggcggtgct atcggcactt
     2821 ggctactggt gtttttaatc tccggctatt cttctttaag tgccgtcttc actgccttgg
     2881 tcgtaccgtt ttacatttgg tggttcgagc cacaatttac tttccccgtc gccttagttt
     2941 gttgcctatt ggtttaccgc catcacgata atattcaacg cttatggcgt gggcaggaag
     3001 acaaactgtg ggataaattc aagaaaaata gagcataaaa ataggcatag tgaaccgttc
     3061 gctatgccta tttgcaaatt attgcggaaa aatcaccgct tgtcttctcg actaaaccgc
     3121 taaatctgct tcatctcctc gattttgtag ccattgttta cgatcttccg ctcgtttttt
     3181 acccaatagc atatccataa tttcaaaggt attcaattcg ccctcttcta ccgtgtcatc
     3241 aaaggttaat tgtactaagc ggcgagtgct tggctccatc gtagtttcac gcaactggct
     3301 cgggttcatt tcgcctaagc ctttaaagcg ttgcacattt ggctttcctt ttttcttggc
     3361 aaggcgagct aaaatcgcct ctttttccgc ctcatctaag gcataatgca cctcatcttt
     3421 gccgatatca atgcgataaa gtggtggcat tgccacataa acgtgtccgt tttgcaccaa
     3481 tttcgggaaa tgacgaagga ataacgcaca cagcaaggtg gcaatatgca aaccgtctga
     3541 atccgcatcg gcaagaatac aaactttgcc ataacgcaac tcttctaaat tatcgctatc
     3601 agggtcaatt cccaaggcta ccgcaatatc gtgtacttct tgtgaagcca atacttggtc
     3661 tgatgaaacc tcccaagtgt ttaaaatctt accacgcagt ggcaaaatgg cttgatactc
     3721 tttatctcga gcttgttttg ccgaaccgcc ggcagaatca ccttctacca agaaaagttc
     3781 ggtacggctg agatcttgag ctgtacaatc cgccagtttg cccggtaaag ccggaccact
     3841 caccaatttc ttacgcacca cttttttcgc cgcacgcata cggctctggg cggaactaat
     3901 tgccatttca gcaattaatt tacctgtctg cacattttgg ttaagccata aactaaaggc
     3961 atctttcaag gtgctatcca catagcttga ggcttgacgg gaagacaaac gctctttggt
     4021 ctgccctgca aattgtggct cttgaatttt cagagaaagc acataagcac aacggttcca
     4081 cacatcatca gcggttaatt ttaccccttt cggcaataaa ttatggattt cgcaaaactc
     4141 caccatcgcc tttaataaac cgttacgcaa gccattaacg tgcgtgccgc cttgtgcggt
     4201 cggaatcagg ttcacatagc tttccgctac taactcgccc ccttccggca accaagttaa
     4261 tgcccagctt accgcctctg tttcagcagt aacatcacca ataaacggtg gattcggcac
     4321 ggtttcataa ccgtttaatg cctcgctcaa ataatcagat aagccgtctt cgtaatacca
     4381 actttcttcg gtattgttga ttttatcaat aaaattgatg gtgagtttcg ggcaaagcac
     4441 cgctttggct cgtaacaaat ggcgtaaacg actcaccgaa aaacgagggc tatcaaaata
     4501 ttttgggttc gggtaaaaac gcactgtcgt gccggtctgt tttttcgggc aagtaccaat
     4561 taccgtcaga tcttccactt ttaccccgtt ggcaaaggca atcgaataga cttctccgcc
     4621 acgtttaatc tgaatttcaa cccgttcgga cagggcattt accaccgaaa tccccacccc
     4681 gtgcaaacca ccggaaaacg tatagttttt attggaaaat ttaccgccgg cgtgcaattt
     4741 cgttaagata agctccaccc ctgaaatttt ctcggtggaa tgaatatcga ccggcattcc
     4801 ccgcccgtta tccaccactt ccaacgaatt atcagcatga agaatcacat caattttatc
     4861 tgcaaagccg gacaacgcct catcgacact attatcaatc acctcctgcc ccaaatggtt
     4921 tggacgtgtg gtatccgtgt acattcccgg gcgaagctgc accggctcga ggtctttcag
     4981 aacggtaatt tcatccgccc catagcgttt ttcagacatt atgctttctc ttaggcttaa
     5041 aataaaataa ggctgaattt taaggaatat tcagctattt tgcaaaattt tggtg
//
LOCUS       DANLYI010000170         4997 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.171, whole genome shotgun sequence.
ACCESSION   DANLYI010000170 DANLYI010000000
VERSION     DANLYI010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4997
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.171"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            286..984
                     /gene="aphA"
                     /locus_tag="PXD81_002051"
     CDS             286..984
                     /gene="aphA"
                     /locus_tag="PXD81_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HDL5538042.1"
                     /translation="MTAATMFASLIAFSAQAGGPKVAYTQPGIDMRTHEAQQAPIHWV
                     SIEQIKESIKDLPPMAVSFDIDDTVAATSGCFHYGKQKYSPDDFSYLKNQDFWDEINA
                     GCDKYSLPKQVAINLIKMHQERGDQVYLITGRTAGKNEQVTTIMQKYLGIKNMQPVNF
                     MGGKAHSTKYDKTPAIIEHKVQIHYGDSDDDILAAREAGIRGIRILRAANSNYTPFPQ
                     AGGYGEEVVVNSSY"
     gene            1165..1926
                     /gene="udp"
                     /locus_tag="PXD81_002052"
     CDS             1165..1926
                     /gene="udp"
                     /locus_tag="PXD81_002052"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HDL5538043.1"
                     /translation="MSEVFHLGLTKAMLKGAKIAIVPGDPARSERIAKKMDNPEFLAS
                     TREFTSWLGYLDGEPVVVCSTGIGGPSVSICVEELAQLGVRTFLRIGTTGAIQEHINV
                     GDILITTGAVRLDGASQHFAPMEYPAVANFECTNALYAAAVESGVTPYVGITASSDTF
                     YPGQERYDTFTGKIYRHFQGSLKQWQDLNVMNFEMESATLFTMCSALGLRAGMVAGVI
                     VNRTQQEIPNEATIHDTEHKAVSVVVEAARKLIKG"
     gene            2001..2597
                     /locus_tag="PXD81_002053"
     CDS             2001..2597
                     /locus_tag="PXD81_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5538044.1"
                     /translation="MKKWLMLITLIFGITACFSSLEFDESKQEKINSFLIVGNKLYVV
                     GELHDYEFEHQQVAELNNFLKSLIANKIKSVEANFEVKESKKVEGSYRIILDGKSLTE
                     SERSTVMDTFHFNSAQNPSITYQAEGNLVKLANREELLQKFRFKQALEANVRYYKMKS
                     GLQNTAEGALSIVMLPVAVVAVLPFYMFWVGACATYGC"
     gene            2608..3093
                     /locus_tag="PXD81_002054"
     CDS             2608..3093
                     /locus_tag="PXD81_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HDL5538045.1"
                     /translation="MYSLYLKILSECDWCCDPKTLSTYQQEWLLHEGSLTQKLYDVAT
                     NFNVEVVQEKWIAKNSEKTTACNEDVWSREVLLKDGEKNWIFAQTLVPRSTIENIAQN
                     VLELGNQPIGLWLFSQNPTRTKLEWTQTENGLFARRGVYEIRGYSLEIRELFLETFPY
                     L"
     gene            complement(3131..4000)
                     /locus_tag="PXD81_002055"
     CDS             complement(3131..4000)
                     /locus_tag="PXD81_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HDL5538046.1"
                     /translation="MKNINTQAVGWIGLGQMGEPMAKHLLNSGVNVGVYNRDPAKAEK
                     VVEAGGTLYPSVLELVKAYDVIFLMIADYAAVQQVLSDEVLAELNGKVVVNMSTISPS
                     QNQAVEALLAQHGAEFVEAPVSGSSKVAEAGKLLVLAAGKEETVELLKPLFAAFSTTT
                     FYYGEVGKAGGIKLMINSLLGIFIQAYGEALNFADQYGIPKEKVIEMISGSFMNSPIF
                     QAKVPMYQANEFPPAFMMKHMTKDFNLASEEIAKLGKSYPLIEQATKTYNQANDSGLS
                     EVDMAAIYQFLAK"
     gene            4130..4921
                     /locus_tag="PXD81_002056"
     CDS             4130..4921
                     /locus_tag="PXD81_002056"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HDL5538047.1"
                     /translation="MKPTILLYDSGMGGLTIYDEIRKVLPHAHYLYCFDNAFFPYSEK
                     SEAVLIERAVKIVQKIAEKQPLDMVVVACNTASTVVLPALREHFNFPIVGTVPAIKPA
                     AAISETKVIGLLATKGTVARPYVDELIRKYAQDCTVEKIGTTDLVEIVEEKQRSGKVD
                     MNRLQKVVEAWQGKPELDTVILGCTHFPLVKAELQQLLPDVKYFVDPGVGIAKRVLSL
                     LEERKFQQKADFENQAFCTKQSSAFNIKQDVMQNWGFNNLSVIDI"
BASE COUNT         1547 a          904 c         1048 g         1498 t
ORIGIN      
        1 tggatcagta tttaatgaat tattagagga gccattttta ttaagcgaat atgatgccta
       61 tcaatttacg aatgaggtga ttaaatttca ttcagaagaa agccttaaag cttggggagg
      121 ttgcttgatt tgggaacaga acaatggctc ataagcgtta agcgtaatcg tttgcttgtg
      181 atcatattca caaaataagg tttttgtatt agctaagagt gcctaataag attaaaatag
      241 gtatggttaa tcatatggag gtattttata aaatttacat taaaaatgac ggcagcgaca
      301 atgtttgcat ctcttattgc tttttcagca caagcaggcg gaccaaaagt agcttatacc
      361 cagccgggaa ttgatatgcg aacacatgaa gcacagcaag cgccaattca ttgggtatct
      421 attgagcaaa ttaaagaaag catcaaagat ttacccccta tggcggtaag ttttgatatt
      481 gatgatactg ttgctgcaac tagcggttgc ttccactacg gaaagcaaaa atattcgcca
      541 gacgatttca gctacttaaa aaatcaagat ttttgggacg aaattaatgc tggctgtgat
      601 aaatattctt tgccaaagca agttgctatt aatttaataa aaatgcacca agaacgtggt
      661 gatcaagttt atttaattac ggggcgtacc gcaggtaaaa atgaacaagt tacaacgatt
      721 atgcaaaagt atcttggtat taaaaatatg caaccggtaa attttatggg ggggaaagca
      781 cattcaacaa aatatgataa aacccctgcg attattgaac ataaggtgca aattcactat
      841 ggtgacagtg atgatgatat cttagctgcc cgtgaagccg gtattcgagg tattcgtata
      901 cttcgtgcag caaattctaa ttatacgcca ttccctcagg ccggaggata tggtgaagaa
      961 gttgttgtca attcaagtta ttaatttctt agtctgaaat atggccacca ttgcgtggcc
     1021 atcatttttt atttatataa tgacgaaacc gtttaccttt gatctagttc acaaaatcaa
     1081 actttgcttg caaaaaattg tcgtaaatta accgcttgct aagatagaat ggctcatatc
     1141 cagttagata aaaaggagat aaaaatgtct gaagtatttc atttaggttt aaccaaagcc
     1201 atgctcaaag gtgcaaaaat tgcaatcgtt ccgggcgatc cggctagaag tgaacgtatt
     1261 gccaaaaaaa tggacaaccc agagttttta gcctcaaccc gtgaatttac ctcttggtta
     1321 ggttatttag atggcgaacc ggtggtggtt tgttcaaccg gtattggcgg accgtcggtg
     1381 tcgatttgtg tggaagagtt agcacagtta ggtgttcgca cattcttacg cattggcaca
     1441 acaggtgcaa ttcaagagca tattaatgta ggcgatattt taattaccac cggagcggtg
     1501 cgtttagatg gtgcgagcca acattttgca ccaatggaat atcctgcagt agcgaatttt
     1561 gaatgtacta atgctcttta tgcagcagca gtggaatcag gcgttacacc ttatgtgggg
     1621 attacagctt catcagacac tttctacccg gggcaagaac gttacgacac tttcaccggc
     1681 aagatctatc gtcatttcca aggttcatta aaacagtggc aagatctcaa tgttatgaat
     1741 tttgaaatgg aatccgccac tctctttacg atgtgttcgg cattaggctt gcgagcaggt
     1801 atggttgctg gtgtaattgt aaaccgcact caacaagaga ttccgaatga agcaaccatt
     1861 cacgacaccg aacataaagc cgtttcggta gtagtggaag cggcaagaaa attaattaaa
     1921 ggctaatctt taaatttaaa agcaaaaaag gtaaattgtc tttgttacaa tttacctttt
     1981 ttctttttag ggagctttct atgaaaaaat ggttgatgtt aattacatta attttcggca
     2041 ttaccgcttg tttttccagt ttagaatttg atgaaagtaa acaagaaaaa atcaattcat
     2101 tcttgattgt tggtaataaa ttatatgtgg tgggtgaact gcacgattat gaatttgagc
     2161 atcagcaagt tgcggaactg aataattttc tcaaaagcct aattgctaat aaaatcaaat
     2221 ctgttgaggc aaacttcgag gttaaggaat ccaaaaaagt ggaaggtagt tatagaatta
     2281 ttttagatgg gaaatcactt acagaaagtg aaagaagtac ggtgatggat acctttcatt
     2341 ttaattcagc tcaaaatcca tctattactt atcaggcgga agggaacttg gtgaaattag
     2401 ccaatagaga agagcttttg caaaaattcc gatttaaaca agcgcttgag gcgaatgtgc
     2461 gttattacaa aatgaaaagt ggtcttcaaa atacggctga aggagcgtta agtattgtaa
     2521 tgttgcctgt tgctgtagta gctgttttac cattctatat gttttgggtt ggtgcttgtg
     2581 ccacttacgg atgttaaata actttttatg tattctctat acctgaaaat tttatcggaa
     2641 tgcgattggt gttgtgatcc aaaaacactt tctacctacc agcaagaatg gcttctgcac
     2701 gaagggtcac ttacccaaaa attgtatgat gtcgcaacca atttcaacgt ggaagtagta
     2761 caagaaaagt ggattgcaaa aaacagtgaa aaaacgaccg cttgtaatga agatgtttgg
     2821 tcgcgagaag tgttactgaa agatggtgaa aaaaattgga tttttgccca aactcttgtg
     2881 ccaagatcaa caatcgaaaa tatcgctcaa aacgtgttgg aattgggcaa tcagccgatt
     2941 ggtttatggc tattttctca aaatcctact cgcaccaaat tagaatggac acaaacagag
     3001 aacggtttat tcgctcggcg tggggtgtat gaaattagag ggtattctct ggaaattcga
     3061 gagctttttt tagaaacctt tccttattta taaaaaaatc ggttggtaat gacaccaacc
     3121 gattttcatc ttattttgcc aagaactgat aaattgcagc catatccact tcgctcaagc
     3181 ctgaatcatt ggcttgattg taggttttag tggcttgctc aataagcggg taagatttgc
     3241 ctaatttcgc aatttcttcg cttgccaaat taaaatcttt ggtcatatgt ttcatcataa
     3301 acgcaggcgg aaattcatta gcttggtaca tcggcacttt tgcttggaag atcgggctgt
     3361 tcataaacga gcctgaaatc atttcgatta ctttctcttt cggaatgcca tattggtcgg
     3421 cgaagtttaa cgcctcgcca taggcttgaa tgaagatgcc cagtagcgag ttgatcatca
     3481 atttaatccc accggcttta cccacttcac cataatagaa agtggtggta ctaaaggcag
     3541 cgaaaagcgg tttgagcaac tcaacggttt cttctttacc ggcagccagt accaatagtt
     3601 tacctgcttc tgccactttg cttgaaccgg aaaccggagc ttcaacaaat tctgcaccgt
     3661 gttgggctag tagagcttct accgcctggt tttggcttgg ggaaatggtg ctcatattca
     3721 ccactacttt gccgtttaat tcagccaaca cttcatcgct taacacctgt tgcacggcgg
     3781 catagtctgc aatcatcaag aaaatcacat cgtaggcttt caccaattcc aacacagaag
     3841 gatataatgt gccgcctgct tcaactacct tttctgcttt tgctgggtcg cggttgtaaa
     3901 cgcccacgtt cactccgcta tttagcaaat gttttgccat cggttcgccc atttgcccta
     3961 aaccaatcca accgactgct tgtgtattaa tgtttttcat tatttttctc cttactgaat
     4021 aaagcaaatt gattattgcg attttccctc atcaagtcaa atagagagaa cttgaatata
     4081 gcaatggaaa agagtagcat tttagcactt acttttttga acaataccaa tgaaaccaac
     4141 aattctttta tacgattccg gaatgggcgg attgacgatt tacgatgaaa tccgaaaagt
     4201 gttaccgcac gctcactatt tatattgctt tgataacgct tttttcccat attccgagaa
     4261 gtcggaagca gttttaattg agcgagcggt caaaattgtg caaaaaattg cagaaaaaca
     4321 accgcttgat atggttgttg ttgcttgtaa tacggcaagc acagtggttc tgccggcatt
     4381 gcgtgagcat tttaattttc cgatagtagg aactgtaccg gcaattaaac cggcagcggc
     4441 aatttccgaa actaaagtca tcggcttgct cgccactaaa ggcacggttg ctcgccctta
     4501 tgttgatgaa ttgataagaa aatatgctca ggattgtacg gtagaaaaaa ttggcacaac
     4561 cgatttggtt gaaattgttg aagaaaaaca acgaagcggt aaagtggata tgaaccgact
     4621 acaaaaagtg gtcgaggctt ggcaaggtaa gccggaattg gatacggtta tactcggttg
     4681 tacccatttt cccttagtca aagctgaatt acagcaatta ttgcccgatg tgaaatattt
     4741 tgttgatccg ggtgtaggta ttgccaaacg ggtgctaagc cttttggaag aacgcaaatt
     4801 tcaacaaaaa gccgattttg aaaatcaagc attctgcact aaacaaagtt ctgcatttaa
     4861 tatcaaacaa gacgttatgc agaattgggg atttaataat ctttcggtga ttgatattta
     4921 aacaaaacaa tctgttttga tgaaaaaagg ttcagttgag caaaaacttt aaaaaagggc
     4981 ttgcactcaa ttttaaa
//
LOCUS       DANLYI010000171         4995 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.172, whole genome shotgun sequence.
ACCESSION   DANLYI010000171 DANLYI010000000
VERSION     DANLYI010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4995
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.172"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            complement(<1..633)
                     /locus_tag="PXD81_002057"
     CDS             complement(<1..633)
                     /locus_tag="PXD81_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HDL5538048.1"
                     /translation="MAEGLLLTPYFQTEVGIVFYKVPKDVSPAAFGERTLLQPAPTDL
                     ATVKSGKVANFSANSTACKELAVFAKSAKVRSTLNQRDKYSNDLPYIRFVKSIQTCQC
                     RDGKYCHTEKVLVENKNGAVQLCWHHDKMRMDGEIPNEKLEALADENWEKFIAQQIRL
                     TLRKTQSTAIEFADLVLFASIKGLLDELDSKALQQFLGYPPLNNSGVSKES"
     gene            complement(760..1353)
                     /locus_tag="PXD81_002058"
     CDS             complement(760..1353)
                     /locus_tag="PXD81_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine methyltransferase"
                     /protein_id="HDL5538049.1"
                     /translation="MTDFDKNTYQTPNYVRNWLNHRYAWFHIDGCSNGQNTLYTYWIG
                     RAADGLDDDKLSCQIADDFLADDLFDVLLDKVSDWGELLRIFVNPPYSDPLPFVQRAA
                     ELKKAGHLVVMLLPADKTTEWYTIIQQHANEVIDIIGYHDEKGTWRTGRIQFINPVTG
                     KPAQGNNKGSMIVVFDPFIEGIVTRQMPLDKIKEWGK"
     gene            complement(1364..2410)
                     /locus_tag="PXD81_002059"
     CDS             complement(1364..2410)
                     /locus_tag="PXD81_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conserved phage C-terminal domain-containing
                     protein"
                     /protein_id="HDL5538050.1"
                     /translation="MRFTSTINNVRLVEWEINITQGALVDLINQASSWAKAVVIDGIT
                     YYWMSFSKVCEELPAVFSKEDTVYRQYKVLKEKGIIDHFKMDGKDYVRLTEKGCEWNK
                     FEPIRESEKNPSIGNNSEQTRKNFRESSEKNPTDNNTNYKNNNDHTTPLNPPAGEPAP
                     AEVVLNYLNSALVTLAVQLGERKPVGYSLKPWAKNITARIRESSVADCCQVVDYLVAK
                     WGRDEKMREYLCPKTIFRQSNFADYFPKSTAWANNGKPVCVNGKWVTPAELEKRLIMP
                     TVDEVRALFQKSLSGNPFKALDFTNKRNLVMYHATINTRNKRPLERDLPMIISQEIKN
                     AVERIDRLRVPTFS"
     gene            complement(2407..3090)
                     /locus_tag="PXD81_002060"
     CDS             complement(2407..3090)
                     /locus_tag="PXD81_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein/antirepressor Ant"
                     /protein_id="HDL5538051.1"
                     /translation="MNQLLNISAPKASITMSSREIAELCEKEHRHVTRDIEAMFSQLD
                     IPAEGYAHFWTHPQNGQTYREFRLPYDLTLTLISGYSVILRKKIIDRWQELESRQGIP
                     QSFSQALRLAAEQQEVIEQQNHQLAIQAPKVAFVEHYVEVGTTKSLRETAKILNFPEK
                     RLIECLERYRVLYRQSGNLLPYQDKQAKQLFAVKTGTAEHGHNFTQTRVTAKGIEWIA
                     QRYASELGQ"
     gene            complement(3148..3336)
                     /locus_tag="PXD81_002061"
     CDS             complement(3148..3336)
                     /locus_tag="PXD81_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HDL5538052.1"
                     /translation="MKKDDVLEHFGTLEKVAATLGISVSAVSQWGEIIPEKNAYRLQE
                     ITAGKLKVKHSLYRNRSK"
     gene            3461..4117
                     /locus_tag="PXD81_002062"
     CDS             3461..4117
                     /locus_tag="PXD81_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL5538053.1"
                     /translation="MNTQTLGDRIKSRRKELKLTQKDLATALKGVSHVAVSQWESSTT
                     KPNSENILDLSTVLQCDISWLLRGNGESNVLPASLGMTKVPLISYIQAGQWTGIDDFR
                     ETCGDYEYILTDLEVSEDAFALEIKGDSMEPDFIEGDRVIIDPDVQPHAGEFVAAING
                     DYEATFKKYRPLEDLDEYGRQHFELIALNPDYHKLSTLKQEIRIIGTMVEHRIYRRKR
                     "
     gene            4129..4503
                     /locus_tag="PXD81_002063"
     CDS             4129..4503
                     /locus_tag="PXD81_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5538054.1"
                     /translation="MTEFKDVKQAIVEAQLASTPKDKVVNDIMKGSAELLSANDIINR
                     LGIGADEFQELVNLPNHYLRINGGLGAAVYNTRRMFDTHVELVNQELEKNISFPKPDL
                     YILGKARWKKETFKKWLEEQCR"
     gene            4655..>4995
                     /locus_tag="PXD81_002064"
     CDS             4655..>4995
                     /locus_tag="PXD81_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5538055.1"
                     /translation="MLSKQVLSISESRLSTYLFCFFRNDPTKQKEAIAIYTALQHRTG
                     IYFSLIQEIEVALRNEVSQLLRFAAPNHNLYEFFHFLANDSNAPLNAESKRQLQKAIS
                     ECGRNYNENDI"
BASE COUNT         1450 a         1028 c          966 g         1551 t
ORIGIN      
        1 actttctttg ctcacaccgc tattattcag tggtggatag ccgagaaact gctgtaatgc
       61 cttgctgtct aattcatcaa gcaaaccttt gatactcgca aacaacacta agtccgcaaa
      121 ttcaatagcg gtagattgcg ttttgcgtaa ggttaagcgg atttgttggg cgataaattt
      181 ttcccagttt tcatccgcta gtgcttctaa tttttcattc gggatttcac cgtccattcg
      241 cattttgtcg tgatgccaac aaagctgtac cgcaccgttt ttattctcta ccagcacttt
      301 ttccgtgtgg caatatttgc catctcggca ttggcacgtc tgaattgact ttacaaaccg
      361 gatataaggc aaatcattac tatatttgtc acgctgattt agcgttgaac gaacttttgc
      421 cgatttagca aacaccgcca gctctttaca agcggtcgaa tttgccgaaa aatttgcaac
      481 cttgcctgat ttaaccgtcg ctaaatcggt cggtgcaggt tgcaacaaag ttcgctcacc
      541 gaaagcagca ggagaaacat ctttaggcac tttgtaaaac acaataccca cttcggtttg
      601 aaagtaaggg gtaagtagca aaccttccgc cattattgcc cccatttctc ggtttcatca
      661 taggctttca cgaatagcat aaatacgcca atacagatta aaaaaatcag tacgccaatt
      721 actgcaccaa taccaataaa tagttcccaa taatccattt tatttacccc attccttgat
      781 tttatctagt ggcatctgac gagttacaat gccctcaata aatgggtcaa acacaacaat
      841 catcgagcct ttattattac cttgtgccgg tttacctgtt accgggttga taaattgaat
      901 ccgccctgtc cgccaagtgc ctttttcatc gtgatagcca ataatatcaa ttacctcatt
      961 agcgtgttgt tgaatgatgg tgtaccattc tgttgtttta tccgctggta ataacatcac
     1021 aaccaaatga cctgcttttt tcaattctgc cgctcgttga acaaaaggca gtggatcaga
     1081 atatggcgga ttaacgaaaa tacgcagtaa ttctccccag tctgaaactt tatctagtag
     1141 cacatcaaat aaatcatcgg ctaaaaaatc atcggcaatt tggcaactta atttatcatc
     1201 atctaaaccg tctgccgctc taccaatcca ataggtgtac aacgtatttt gtccgttaga
     1261 gcaaccgtca atatgaaacc aagcataacg atgatttagc caattacgca cataattagg
     1321 tgtttggtaa gtgtttttat caaaatctgt cattgtctaa tccttacgaa aatgttggta
     1381 ctctcaaacg gtcgatacgc tcaaccgcat ttttaatttc ttggctgata atcatcggca
     1441 aatcacgttc aagtggtcgc ttgtttctgg tgttgattgt ggcgtggtac atcactaaat
     1501 ttcgtttgtt ggtaaagtcc aatgccttaa acggattgcc actgagtgat ttctggaaca
     1561 aggctcgtac ttcgtccacc gttggcataa tcaagcgttt ttccagctcc gccggtgtaa
     1621 cccacttacc atttacgcaa acaggctttc cgttattagc ccaagcggtc gattttggaa
     1681 aataatctgc aaaattcgat tggcggaaaa tcgttttcgg gcagaggtat tcacgcattt
     1741 tttcatctcg tccccattta gcgacaaggt aatccaccac ttggcaacag tccgccaccg
     1801 aactttcacg aatgcgagca gtaatatttt ttgcccacgg cttgagcgaa taccccacag
     1861 gcttacgctc accaagctgt accgccaacg ttaccaaagc cgaattgaga taattcaaca
     1921 caacttcagc cggggcaggt tcccccgctg ggggattaag gggggtagta tgatcattat
     1981 tatttttata attagtatta ttatctgtcg gatttttttc cgagctttct cggaaatttt
     2041 tccgagtttg ttcggagtta tttccgatac tcggattttt ttccgactca cggatcggct
     2101 cgaatttatt ccattcacaa cctttttcgg ttaaacggac ataatcttta ccgtccattt
     2161 tgaagtggtc gataatgcct ttttctttca acaccttata ttggcggtaa actgtatctt
     2221 ctttgctaaa aaccgcaggt aactcttcgc aaaccttgct aaatgacatc cagtaatagg
     2281 ttatgccgtc aataaccacc gctttcgccc aactggaggc ttgattgatt aaatcgacca
     2341 atgccccttg tgtaatattg atttcccatt ccactaagcg gacattgttt atggttgatg
     2401 taaatctcat tgtcctaact ccgatgcgta acgttgagca atccattcaa tccctttggc
     2461 ggttactctc gtttgggtaa aattatgacc gtgttctgcc gtgccggttt ttactgcaaa
     2521 aagctgttta gcttgtttat cttgataagg gagtaggttt cctgattggc gataaagaac
     2581 tcgatatcgt tctaaacatt caatcaaccg cttttctgga aaattgagta tcttggcggt
     2641 ttctcgcaat gatttagtcg ttcccacctc aacataatgc tccacaaatg cgactttcgg
     2701 ggcttgaatg gctaattgat gattctgctg ctcaatcact tcttgctgtt ctgccgccaa
     2761 ccgtagagct tgagagaaag attgtggaat accttgtctg ctttctagct cttgccaacg
     2821 gtcgataatt ttcttgcgaa gtatcacgct atagccggaa attagggtaa gggttaaatc
     2881 gtaaggcaat cgaaattccc ggtatgtttg cccattttga gggtgtgtcc aaaaatgggc
     2941 atacccctcg gcaggaatat ctaattgact aaacatcgct tctatatcac gagtaacgtg
     3001 tcgatgttct ttttcgcaca actcagcaat ctcacgacta ctcatcgtaa tgcttgcttt
     3061 tggtgccgaa atgtttaata attgattcat cgaaaatacc tttcgtgttt aattatgtat
     3121 gaaagccacc gttacagcgg tggtttttta tttgcttcta tttctataaa gtgagtgttt
     3181 gacctttaac ttgccggcag tgatctcttg taatctgtac gcatttttct caggaataat
     3241 ctcgccccat tgagaaacag cagatacact aatccctaaa gttgccgcca ctttttctag
     3301 agttccgaaa tgttcgagca catcatcttt tttcatattt tcttaattcc tttcttgaat
     3361 tttatttaag acctcttaaa tatacaatta aaggaaactt aagtcaataa atgttaagat
     3421 aacttaacag caactttatt tttaccttta aggggatact atgaatactc agaccttggg
     3481 cgatagaatt aaaagccgta gaaaagaatt aaaattgaca cagaaagatc ttgctactgc
     3541 gttaaaaggg gtctcacacg ttgcagtatc tcaatgggaa agtagtacga ctaagccaaa
     3601 ttcagagaat attttggatt tatctacagt attgcaatgc gatattagtt ggctcttgcg
     3661 tggtaatgga gagtctaatg ttctacctgc tagcctcggt atgactaaag tcccattaat
     3721 tagctatatt caagccggac aatggacggg aattgacgat tttcgtgaaa cctgtgggga
     3781 ttatgaatat atcctaactg atcttgaggt gtctgaagat gcttttgccc tagaaatcaa
     3841 aggggactcg atggagcctg attttattga aggagaccga gtaattattg atcctgatgt
     3901 acaacctcac gccggtgagt ttgtggcagc aattaatggc gattatgaag ccactttcaa
     3961 aaaataccgt ccgttagaag acttagacga atacggaaga caacattttg agcttatcgc
     4021 tcttaatccg gattaccata aattatcaac gctaaagcaa gaaatcagaa ttatcggcac
     4081 aatggtagag catcgtattt atcgtagaaa gagataggaa ttgataatat gacagaattt
     4141 aaagatgtaa aacaggcgat tgtagaagct caattagctt cgacacctaa agataaagtc
     4201 gttaatgaca taatgaaagg aagtgcggaa ctgctttcag caaatgatat tatcaatagg
     4261 ttaggtattg gggcagatga gtttcaagag ttggttaatt tacctaacca ttatttgcgt
     4321 ataaatggag ggttaggtgc tgctgtgtat aatacaagaa gaatgtttga tacccatgtt
     4381 gagttagtca atcaagagtt agaaaagaat atttcatttc ctaaacctga tttatatatt
     4441 ttggggaaag ctcgttggaa aaaagaaaca tttaagaaat ggttggaaga acagtgtaga
     4501 taaagaaaag gtaggttaaa aaacctacct tttctgaacc gcaaataagc gattctcaca
     4561 ctttacaaag caatccagct cctacgtctg gggcttcggg tccgttgcca gtgcaagtat
     4621 tattacatta aactaaactt gagtaaagga aaatatgttg tccaaacaag tgctaagtat
     4681 ttcagaaagt cggctgagta catatttatt ttgctttttc cgaaatgatc ccacaaaaca
     4741 gaaagaagct attgcaattt ataccgcctt gcaacatcga acaggaattt atttttcatt
     4801 gattcaagaa atagaggtgg cactaagaaa tgaagtcagc cagttgttac gctttgctgc
     4861 acctaatcat aatttatacg aattctttca ttttctagcg aacgatagta atgctcctct
     4921 taatgcagaa tctaaacgcc agctacaaaa agccattagt gaatgcggac gtaactataa
     4981 tgaaaatgac ataat
//
LOCUS       DANLYI010000172         4883 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.173, whole genome shotgun sequence.
ACCESSION   DANLYI010000172 DANLYI010000000
VERSION     DANLYI010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4883
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.173"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            complement(<1..870)
                     /locus_tag="PXD81_002065"
     CDS             complement(<1..870)
                     /locus_tag="PXD81_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012262680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide transport protein"
                     /protein_id="HDL5538056.1"
                     /translation="MNKLTQLALISLAFTGSAFAAPKSFTYCLESSPAALNPQIATDG
                     ATLDVGQAIYNRLIDFEKGTTNLTPSLAERWEISEGGKIYTFFLRQNVQFQSNKSFTP
                     SRYFNADDVIFSINRQFDKTNPYHKLSGGNYEFFYGMNMQNIIKNVTKVDDYTVKIEL
                     SEPNAPFLANLAMDFMAIFSAEYANKMQIAGTPEKLDLEPIGTGAFQFYGYQKDAAVR
                     FTAFEHYWEGKAKIDRLVFSITPDASVRLAKLEKNECQAMPYPNPSDLSALKANKDIQ
                     LFEKSGLNIGYITF"
     misc_feature    complement(896..1041)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            complement(1218..2228)
                     /gene="mglC"
                     /locus_tag="PXD81_002066"
     CDS             complement(1218..2228)
                     /gene="mglC"
                     /locus_tag="PXD81_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018355241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HDL5538057.1"
                     /translation="MSALQQNRTLDFFKQNAIYFVLLILLIVIIVQDPSFLSLRNFSN
                     ILSQSSVRLIIALGVAGLIVTQGTDLSAGRQVGLAAVISATLLQSMENMNKVFPNLSE
                     IPIPVVVLTVCAIGAVIGLFNGFVVAYLNVTPFIATMGTMIIVYGINSLYYDAVGSSP
                     IAGFSDDFSTFAQGFFRFGDFRLSYITIYAVIATIIMWTLWNKTRFGKNVFAIGGNPE
                     AARVSGVNVPLNLMGIYMLSGIFYAFGGMLEAGRIGSATNNLGFMYEMDAIAACVVGG
                     VSFAGGVGTIIGVVTGVLIFTVINYGLTYIGVNPYWQYIIKGGIIILAVAIDSLKYAK
                     KK"
     gene            complement(2242..3741)
                     /gene="mglA"
                     /locus_tag="PXD81_002067"
     CDS             complement(2242..3741)
                     /gene="mglA"
                     /locus_tag="PXD81_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005709009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HDL5538058.1"
                     /translation="MTGEVLLTMTNVSKSFPGVKALDNANLTVKSHSVHALMGENGAG
                     KSTLLKCLFGIYAKDEGDILFLGKPVNFKTSKEALENGISMVHQELNLVKQVTVMDNL
                     WLGRYPLKGPFVDHDKMYRDTKAIFDELDIDVDPRDKVAKLSVSQMQMIEIAKAFSYN
                     AKIVIMDEPTSSLSEKEVEHLFKIIQKLKDRGCGIIYISHKMDEIFKICDEITILRDG
                     KWVNTVAIKDTSMDQVVSMMVGRELTQRFPEKTNLPKEVILEVEHLTAKNQPSIQDVS
                     FSLRRGEILGIAGLVGAKRTDIVETIFGVREKAGGTIRLNDKEINNRNAFDAINNGFA
                     LVTEERRSTGIYANLSIEFNSLISNMKSYLGKFGLLSNKKMKSDTQWVIDSMNVKTPS
                     HQTHIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDIGAKFEIYQLILELANKDK
                     GIIMISSEMPELLGVTDRILVMSNGKVAGIVETAHTSQEEILQLAAKYL"
     gene            complement(3813..4802)
                     /gene="mglB"
                     /locus_tag="PXD81_002068"
     CDS             complement(3813..4802)
                     /gene="mglB"
                     /locus_tag="PXD81_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015701875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HDL5538059.1"
                     /translation="MKKAVLSAVAMAVALGATISTAQAENRIGVTIYKYDDNFMALMR
                     KEIEKEGKTQKVELLMNDSQNTQSIQNDQVDVLLSKGVKALAINLVDPSAYKTIIQKA
                     KSEDVPVVFFNKDPGAAAIDSYPKAYYVGTEPKESGIIQGELIAKQWKAHPELDLNKD
                     GKIQFALLKGEPGHPDAEARTKYVVESLKAQGIDSEQIFMDAALWDAAQAKDKVDAWL
                     SSGKAKDIEVIISNNDGMALGALEATKAHGRKLPIFGVDALPEALQLIKKGELAGTVL
                     NDGVNQGKAVVQLSANLADGKDAVAGTQWKLENRVVRIPYVGVDKDNLSEFLK"
BASE COUNT         1417 a         1048 c          882 g         1536 t
ORIGIN      
        1 aaaagtaata taaccgatat ttaggcctga cttttcaaat aattgaatat ctttattcgc
       61 ttttaaagca gacaaatctg acgggttcgg ataaggcatc gcttggcatt cgttcttctc
      121 caatttagct aaacgtactg aggcatcggg cgtaatacta aatacaaggc gatctatctt
      181 cgcttttcct tcccaatagt gttcaaatgc tgtaaatctt accgctgcgt ccttttggta
      241 accatagaac tggaatgcac ctgtgcctat aggttctaaa tctaattttt ccggcgtacc
      301 cgctatttgc attttatttg catattccgc agaaaagatc gccataaaat ccatagctaa
      361 attagcaaga aagggggcat tcggttcaga taattcgatt tttaccgtgt aatcatctac
      421 ttttgttaca tttttgatga tgttttgcat attcattccg tagaaaaact catagttacc
      481 gccggacaat ttgtgatatg gattcgtctt atcaaactga cggttaatcg aaaaaataac
      541 atcatcagca ttaaaataac gactaggggt aaaactttta ttactctgga actgcacatt
      601 ctgacgcaag aaaaaagtat aaatttttcc gccttcactg atttcccatc gctctgccag
      661 agaaggagtg aggttagtgg tacctttttc aaaatcaatc aggcgattat agattgcttg
      721 cccgacatca agagtagctc catctgttgc aatctgagga ttcaatgctg ccggagatga
      781 ttccaaacag taagtaaaag atttaggtgc tgcgaaagca gaacctgtaa atgctaaaga
      841 aataagtgca agttgtgtca gcttgttcat tgtcatatcc ttaaatgtta agcaataaaa
      901 aaccctttca aaagttgcct tccgaaaggg ttagaattgg gtttgcgatt ttggattgcg
      961 tttgccattt caggttttcg gaagatatct tccgaacacg ccaaatgccc gaaagattaa
     1021 tcggtcaaat ggtagtgatg atgtttaacg caatagctca taatattctc caaaattgaa
     1081 tgtttaggaa tttacgcttt ttaaaaatca attgcaaacg ttttctttaa aattttgtaa
     1141 aaaattcaac atttttcacc gcttgtatcg tacctaggag caaatggcga tttgctccta
     1201 ggtttccaag caataggcta ttttttcttc gcatatttaa gtgagtcaat cgctaccgcc
     1261 aagataatga tgccaccttt aataatatat tgccagtaag ggtttactcc aatataggtt
     1321 aagccgtagt tgataacggt gaagattaac acccctgtta ctacaccgat gatagtaccc
     1381 acaccaccgg caaaggatac cccacctacc acacaagcgg caatggcatc catttcatac
     1441 ataaagccta agttgttggt tgcactaccg atacgacctg cttctaacat accgccgaag
     1501 gcatagaaga tgcctgaaag catataaata cccatcaggt taagcggtac gtttacaccg
     1561 gaaactcgtg ctgcttcagg gttgccgcca atagcaaata cgtttttacc gaagcgggtt
     1621 ttattccata acgtccacat aataatggta gcaatcacgg cataaatagt gatgtaggat
     1681 agcctaaagt cgccaaagcg gaagaagcct tgtgcaaagg ttgagaaatc atcgctaaag
     1741 cctgcaattg gcgatgagcc tactgcatcg tagtaaagcg agttgatacc atacacaata
     1801 atcattgttc ccatcgtggc aataaacggt gttacgttga ggtaagccac cacaaagccg
     1861 ttaaataaac caatgacagc accgatggca cacacggtta atacaaccac aggaatcggg
     1921 atttccgaga ggtttgggaa taccttgttc atattttcca ttgattggag caaggtggcg
     1981 gaaattaccg ctgccaagcc cacttggcga ccggcagata agtctgtccc ttgtgttaca
     2041 atcaagcctg caacccctaa tgcaataatc aaacgcacag aagattgcga taaaatgtta
     2101 ctgaagttac gcaagctcaa gaaacttggg tcttgcacaa taatcacaat aagtaagatt
     2161 aataatacga agtaaatcgc attttgttta aaaaaatcga gtgttctatt ttgttgtaaa
     2221 gcagacatac ttcactcctt gttataaata ttttgctgcg agttgcaaga tttcttcttg
     2281 cgaggtgtga gcggtttcga caatacctgc aactttcccg ttactcatca ctaaaattcg
     2341 gtctgtgacc ccaagcaatt ccggcatttc cgatgaaatc ataatgatgc ctttatcttt
     2401 attcgccagc tctaaaatca actggtaaat ttcaaatttt gccccgatat cgataccacg
     2461 ggtcggctcg tcaagcatca agatttcagg ctgggttaac aaccaacgcc cgataatcac
     2521 tttttgttgg ttaccgccgg aaagtgagcc aatatgcgtt tgatgcgatg gggtttttac
     2581 attcatcgaa tcaattaccc attgggtatc gcttttcatt tttttattgc tcagtaagcc
     2641 gaacttgcct agataagact tcatatttga aatcagagag ttaaactcaa tgcttaagtt
     2701 ggcataaatc cctgttgaac ggcgttcttc tgtaaccaat gcaaagccgt tattaatcgc
     2761 atcaaaagcg ttgcggttat tgatttcttt atcgtttagt cggatagtgc cgcctgcttt
     2821 ctcccgtaca ccgaaaatgg tttcaacaat atcagtccgt tttgctccaa ctaagccggc
     2881 gatgcctaaa atttcccctc tgcgtaagct aaagctgaca tcttgaatag agggttgatt
     2941 ttttgccgtt aaatgctcaa cttcaagaat cacttctttc ggcagattgg ttttttcagg
     3001 gaaacgctgg gtcagctcac gccctaccat cattgaaacc acttgatcca ttgaggtatc
     3061 tttaattgca acggtgttta cccatttacc gtcacgcaaa atggtaattt catcacaaat
     3121 tttaaaaatt tcgtccattt tgtgtgaaat atagataatg ccacagcctc tgtctttcag
     3181 tttttggata attttaaata aatgctcgac ttctttttct gaaagtgacg aagttggttc
     3241 gtccataatc acaattttgg cattgtaaga gaaagctttt gcaatctcga tcatctgcat
     3301 ttgtgaaacc gataatttgg ctactttatc acgtggatca acatcaatat ctaattcatc
     3361 aaaaatcgcc ttagtatcac gatacatttt atcgtgatct acaaaaggcc cttttagtgg
     3421 gtaacgacct agccataagt tatccattac cgtcacttgt tttactaggt tcaactcttg
     3481 gtgaaccatt gagatcccat tttctaacgc ctcttttgag gtcttaaaat tcaccggctt
     3541 gccaagaaag agaatatctc cctcatcttt agcataaatc ccaaataagc atttcagtaa
     3601 cgttgattta cctgctccat tctctcccat taaggcgtga acactatggg atttcaccgt
     3661 taagttagca ttatctaagg cttttacccc gggaaatgac ttgctaacat ttgtcattgt
     3721 gagtaaaact tcacctgtca tatctcaccc ctgattacat cgaaaaatcg aaaaaaagca
     3781 cgggtatcca tcacccgtgc taaaagcgtg aattatttta aaaattcaga taagttgtct
     3841 ttatcgacac caacgtaagg aatacgcacc acacggtttt ccaatttcca ttgtgtacca
     3901 gcaactgcat ctttaccgtc tgctaagtta gcagaaagct gaacaaccgc tttaccttgg
     3961 tttacaccat catttaatac cgtaccggct aattcacctt ttttgattaa ttgaagtgct
     4021 tctggcaatg catctacacc gaagattggt aattttctac cgtgagcttt ggttgcttct
     4081 aatgcaccta acgccatacc atcattatta gaaataatta cttcgatgtc tttcgcttta
     4141 ccacttgata accaagcatc cactttatct ttagcttggg ctgcatccca taatgctgcg
     4201 tccataaaga tttgctcact gtcgatacct tgtgctttta agctttctac cacatattta
     4261 gtacgtgctt ccgcatcagg gtgacccggc tcacctttta ataaggcaaa ttggatttta
     4321 ccgtctttat tcaagtctaa ttcaggatgg gctttccact gttttgcaat taactcacct
     4381 tgaataatgc ctgactcttt cggctcagta cctacataat aagctttcgg atagctatca
     4441 atcgcagctg cacccgggtc tttattgaag aacaccacag gcacatcttc actttttgct
     4501 ttttgaataa ttgttttata agctgatgga tccactaagt taattgctaa tgctttaacc
     4561 cctttagaaa gtaatacatc aacttggtcg ttttgaatag attgcgtatt ttgcgagtca
     4621 ttcattaata actcgacttt ttgtgtttta ccctcttttt cgatttcttt acgcattaat
     4681 gccataaagt tatcgtcata tttataaatt gttacaccaa tacggttttc agcttgagct
     4741 gtactgatcg ttgcacctaa tgcgactgcc attgccactg cacttaatac tgctttcttc
     4801 atagtagatc acctctttta tgtgtttata gggaaatgaa ttgcttacca taagcaactt
     4861 ggtgcaaagt ataactaatt tta
//
LOCUS       DANLYI010000173         4841 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.174, whole genome shotgun sequence.
ACCESSION   DANLYI010000173 DANLYI010000000
VERSION     DANLYI010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4841
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.174"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            13..498
                     /locus_tag="PXD81_002069"
     CDS             13..498
                     /locus_tag="PXD81_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein CcmH"
                     /protein_id="HDL5538060.1"
                     /translation="MKIKTLSLIMLLSVTVNAAPVELHQFDSPKQESDYRALIQELRC
                     PQCQNNNIADSNATIATDMRAKTLELLKQGKSKDEVVDYMIERYGNFVTYDPPLTPAT
                     IMLWLLPLILIALGLGVVLRRKKNKPQAVNFEQNTAKPTTHLAQDEQERLNQILNKEE
                     K"
     gene            495..1406
                     /gene="ccmI"
                     /locus_tag="PXD81_002070"
     CDS             495..1406
                     /gene="ccmI"
                     /locus_tag="PXD81_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcmI"
                     /protein_id="HDL5538061.1"
                     /translation="MNFWLAIIFVTLIVCGVAFYPLLKKNKQIDNTKRDSLNKAFYFD
                     RLKEVENEANEGVIDDPEQTKLELQKNLLDDIPHSTQSATSEKLTLGKSWFVALILFV
                     GGISFAIYSNVGSWFSNSMLDTTHQKLDSFYERLKTEETNPLSQEEMNQFAMALRVDL
                     QSNPNDDKKWYMLGQIGMALDDGQLAHDAFSKAAQLKPDNLDYKLSHAQLLLFSEAQE
                     DKKQGETLLKEIIRQDHTNFNALSLLAFHSFEKEDFKMAAMTWGMMLKLLPDEDPSKV
                     TVERSFNMALSNLSEAEKQELQKGEKK"
     gene            1403..2008
                     /gene="mog"
                     /locus_tag="PXD81_002071"
     CDS             1403..2008
                     /gene="mog"
                     /locus_tag="PXD81_002071"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HDL5538062.1"
                     /translation="MTACKPLSKEKLKIGLVSVSDRASQGVYSDQGIPELRAWLESAL
                     TEPFELETRLIPDEQAIIEQTLIELVDAHACHLVLTTGGTGPAKRDVTPDATLAVAHR
                     EMPGFGEQMRQVSLHFVPTAILSRQVGVIRHDSLILNLPGQPKAIKETLEGVKDEQGK
                     VLVKGIFSAVPYCLQLLSEIYVETHAAICESFRPKSARREN"
     gene            2018..2356
                     /gene="glnB"
                     /locus_tag="PXD81_002072"
     CDS             2018..2356
                     /gene="glnB"
                     /locus_tag="PXD81_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005654045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HDL5538063.1"
                     /translation="MKKIEAIIKPFKLDDVREALTDVGVSGMSITEIKGFGRQKGHTE
                     LYRGAEYAIDFLPKVKVEIVIPDELEEQCIEAIMETAQTGKIGDGKIFVYDVGRVIRI
                     RTGEENEEAI"
     gene            2437..2916
                     /gene="lspA"
                     /locus_tag="PXD81_002073"
     CDS             2437..2916
                     /gene="lspA"
                     /locus_tag="PXD81_002073"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005724521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HDL5538064.1"
                     /translation="MKTGLKFLWVSLIVIIIDLWSKYIVVQHFEFGESINILPIFNFT
                     YARNYGAAFSFLADHSGWQKYFFLGLAVVISSALVVALWKNQAVKKLENIAYALIIGG
                     AIGNAIDRAYNGYVVDFLHFYWDIYHYPVFNIADIAICVGAGLLILEAFITKKEPKK"
     gene            2927..3871
                     /gene="ispH"
                     /locus_tag="PXD81_002074"
                     /gene_synonym="lytB"
     CDS             2927..3871
                     /gene="ispH"
                     /locus_tag="PXD81_002074"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012263266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HDL5538065.1"
                     /translation="MKILLANPRGFCAGVDRAISIVELALEIHGAPIYVRHEVVHNKF
                     VVDGLKAKGAIFVEELHEVPDDAIVIFSAHGVSQVVRQEAKNRGLKVFDATCPLVTKV
                     HMQVARASKKGTKAILIGHEGHPEVIGTMGQYNNQEGGIYLVESVEDIANLPVSNNDE
                     LTFMTQTTLSIDDTIGVIEALKEKYPAIQGPRKNDICYATTNRQHAVRELAEQSQLVL
                     VVGSKNSSNSNRLAELASRMGVMSKLIDGPQDIDPTWLEGIETIGITAGASAPEVLVQ
                     SVISHLKTLGVTEVETLVGNEEDVVFEVPKELRIKEVK"
     gene            3871..4119
                     /locus_tag="PXD81_002075"
     CDS             3871..4119
                     /locus_tag="PXD81_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase assembly factor 2"
                     /protein_id="HDL5538066.1"
                     /translation="MTEINRFKLEWACRRGMRELDKMIMPFYQNHFNELNETQQQAFV
                     EMLNYSDPELFRWVMLQAPAPTEEIAELIRLMRDKIET"
     gene            complement(4326..>4841)
                     /locus_tag="PXD81_002076"
     CDS             complement(4326..>4841)
                     /locus_tag="PXD81_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDL5538067.1"
                     /translation="SANQYIFNSDWFTHNVPSLASIFEYVKPRRILEIGSFEGRSSVF
                     FIEEALKYQSQVEIHCIDSWEGGAEHQGVWDMGEIEQRFIHNVTLAQQKFQNVVLYKH
                     RGYSHQKMIELLANGYINYFDYIYIDGSHEAPDVLFDALLAHRLVRKGGVISFDDYLW
                     SPDETGKQRHY"
BASE COUNT         1529 a          977 c         1043 g         1292 t
ORIGIN      
        1 atattcaaaa ttatgaaaat aaaaactctc agcctaataa tgcttctaag tgtaacagtt
       61 aatgctgctc cggtagaact gcaccaattc gacagcccga aacaagagtc ggactatcgt
      121 gccttaattc aagaactgcg ttgcccgcag tgtcaaaata ataatatcgc cgattccaat
      181 gcgactattg ccaccgatat gcgggcaaaa accttagaat tgctcaaaca aggcaaaagc
      241 aaagacgaag tggtggatta tatgattgaa cgctacggca atttcgtcac ttacgacccg
      301 cctctcacac cggcaaccat tatgttatgg ctgttgccgt taattttaat tgccttaggg
      361 ctgggggtgg tgttgcgtag aaagaaaaac aaaccacaag cggtcaattt tgagcaaaat
      421 actgcaaaac cgaccactca tctcgctcaa gatgagcaag aacgtcttaa ccaaattctc
      481 aataaggaag aaaaatgaat ttctggctgg caattatttt cgttacactg attgtatgtg
      541 gcgtggcgtt ttatccgtta cttaaaaaga acaagcaaat cgataacacc aagcgagaca
      601 gcttaaacaa agccttctat tttgaccgct taaaagaagt ggaaaacgaa gcgaatgaag
      661 gggtgattga tgaccctgaa caaaccaaat tagagctaca aaaaaacttg cttgatgata
      721 ttccacacag cacacaaagt gccacaagtg aaaaattaac gctcggcaag tcgtggtttg
      781 tggcattgat tttatttgtc ggcggtatca gctttgcgat ttacagcaat gtcggttctt
      841 ggttctccaa ctcaatgtta gacaccactc accaaaaatt agacagcttc tatgagcgtt
      901 taaaaaccga agagaccaat ccgctctcac aagaggaaat gaaccaattt gcgatggcat
      961 tacgggtaga tttacaaagt aatccgaatg acgacaaaaa atggtatatg ctcggtcaaa
     1021 tcgggatggc gttagatgat ggtcagcttg cacacgatgc ctttagcaaa gcggctcaac
     1081 tcaagccgga taacttagat tacaaactca gccacgccca actgctgctc ttttccgaag
     1141 cacaggaaga caaaaaacag ggtgaaacct tgttaaaaga gattatccgc caagaccaca
     1201 caaactttaa tgcgttaagt ttattggcgt tccactcctt tgaaaaagaa gatttcaaaa
     1261 tggcggcaat gacgtgggga atgatgttaa aactactgcc tgatgaagat cctagcaaag
     1321 taacggtaga acgcagtttt aatatggcgt tatccaattt aagtgaggcg gaaaaacaag
     1381 aattgcaaaa aggtgagaaa aaatgaccgc ttgtaaacca cttagcaaag agaagttgaa
     1441 aatcggcttg gtgtcggttt cagaccgagc ctcgcagggg gtttattcag accaaggcat
     1501 tcccgaatta agggcgtggc ttgaatctgc cttaaccgag ccgtttgagc tggaaacccg
     1561 cttaattccc gatgagcaag ccattattga gcaaacctta atcgagttag tcgatgctca
     1621 cgcttgccat ttggtattaa ccaccggcgg caccggcccg gcaaaacgtg atgtcacccc
     1681 agatgccacc cttgctgtcg cccaccgaga aatgcccggc tttggcgaac agatgcgaca
     1741 agtgagcctt cacttcgtgc caaccgccat tctctctcgc caagtgggag taattcgcca
     1801 cgacagtctg attttaaatc tgccggggca accgaaagca attaaagaaa cgctggaagg
     1861 tgtgaaagat gaacagggca aagtattggt taaaggtatt tttagtgccg tgccatactg
     1921 tttacaactt ttaagcgaaa tttatgtcga aacccacgca gctatttgcg aaagtttcag
     1981 acccaaatcg gcaagacgag aaaattaagg ctaaaaaatg aaaaaaatag aagcaattat
     2041 taaacctttt aaattagacg atgtgcgtga agccttaacc gatgtgggtg tcagcggaat
     2101 gagtattacc gaaatcaaag ggtttggcag acaaaaagga cataccgagc tttatcgtgg
     2161 ggcggaatat gcgattgatt ttctaccgaa agtgaaagtc gaaatcgtga ttcccgacga
     2221 gttggaagaa cagtgcattg aagcaattat ggaaaccgcc caaaccggca aaatcggcga
     2281 tggcaagatt tttgtgtatg atgtcgggcg ggtgatccgc attcgtaccg gcgaagaaaa
     2341 tgaagaagcg atttagtttt gcaaaaaatc cggcaaaaac gaccgcttgc agggggattt
     2401 ccatctactc tttttacatt aaattttgga tattaaatga aaacaggctt aaaatttctt
     2461 tgggtgagtt taattgtaat catcattgac ctttggtcaa aatacattgt agtgcaacat
     2521 tttgaatttg gtgaaagcat taatattttg ccgattttta acttcactta cgcccgaaac
     2581 tacggggctg cctttagttt ccttgccgac cattccggct ggcaaaaata tttcttttta
     2641 gggctggcgg tagtgatttc cagtgcttta gtggtggctc tttggaagaa tcaagcggtc
     2701 aaaaaattag aaaatattgc gtatgcctta attattggtg gagcgattgg gaatgccatt
     2761 gaccgtgctt acaacggcta tgtagttgat ttccttcatt tttattggga tatttatcac
     2821 taccctgtgt ttaatattgc cgatattgcg atttgtgtgg gtgcaggctt actgatttta
     2881 gaagccttta taaccaaaaa agaaccgaaa aaataagagt gattaaatga aaattttatt
     2941 agcaaaccca cgcggttttt gtgccggcgt ggatcgagcc atttcgattg ttgaattagc
     3001 cttagaaatt cacggtgcac ctatctatgt tcgccacgaa gtggtacata acaaatttgt
     3061 ggtggatggc ttgaaagcca aaggggctat ttttgttgag gagctacacg aagtgccgga
     3121 cgatgctatc gtgatcttct cagcccacgg ggtgtcgcaa gtagttcgcc aagaagccaa
     3181 aaatcgtggc ttaaaagtgt ttgatgccac ttgcccactc gtgaccaaag tgcatatgca
     3241 ggttgcccga gccagtaaaa aagggacaaa agcaatttta atcggtcacg aaggtcaccc
     3301 tgaagttatc ggcacaatgg gacaatacaa taaccaagaa ggtggcattt atttggtaga
     3361 atcggtggaa gatattgcca acttgccggt ttccaacaac gatgaattaa cttttatgac
     3421 gcaaaccaca ctttccattg acgataccat tggcgtgatt gaggcgttaa aagagaaata
     3481 tccggcaatt caaggccctc gcaaaaatga tatttgttat gctaccacca atcgccaaca
     3541 cgcagtgcgg gaactggcag aacaatcaca attagtgtta gtggtaggtt caaaaaactc
     3601 ctcgaactca aatcgtttgg cggaactcgc ctctcgaatg ggtgtgatgt caaaactgat
     3661 tgatggtccg caagatattg atccaacttg gttggagggt attgaaacta tcggtattac
     3721 cgccggtgct tccgctcctg aagtgttggt gcaatcagta atttcgcatt taaaaaccct
     3781 tggcgtaacc gaagttgaaa ccttagtcgg caacgaagaa gacgtggtgt ttgaagtccc
     3841 gaaagagtta cgcatcaaag aggtaaaata atgacagaaa tcaaccgctt taaattagaa
     3901 tgggcgtgcc gacgtggtat gcgtgagtta gacaaaatga ttatgccgtt ttaccaaaat
     3961 cattttaatg aactcaatga aacccaacag caagctttcg ttgaaatgct caattacagc
     4021 gacccggaac tgtttcgctg ggtaatgctg caagcacctg ccccaaccga agaaatcgct
     4081 gaattgatta ggttaatgcg agataaaatt gagacctaga gctaatagat taacctcttc
     4141 tttggggtaa atatatagct acttacccca aagaagacta aattcattca acttttacaa
     4201 aataacccta aactagaaaa gttaatcttc caatttaata atatgttgct gataagctat
     4261 taaaccttgt attacatgca ctttctgctg ataggtattg acatagtgat caacagcggg
     4321 ttttactaat aatgacgctg tttacctgtc tcatctggtg accaaaggta atcatcaaaa
     4381 ctaataacgc ctccttttct cactaaacga tgtgcaagta aagcatcgaa caacacatct
     4441 ggcgcttcgt gagatccatc tatgtaaata tagtcaaaat aatttatata tccatttgcg
     4501 agaagttcaa tcattttttg gtgagaatag ccacggtgct tatataacac aacattttgg
     4561 aatttctgtt gcgcaagagt gacattatgg ataaatcgtt gttctatttc tcccatgtcc
     4621 caaactcctt gatgctctgc tcccccttcc caactatcaa tacaatgaat ttcaacttgt
     4681 gattgatatt tcaatgcttc ttcaatgaaa aagacagatg atctaccttc aaatgatcca
     4741 atttctaaaa tcctccgagg ttttacatat tcaaaaatag aagctaatga aggaacatta
     4801 tgagtaaacc aatcagagtt aaaaatatat tggttggctg a
//
LOCUS       DANLYI010000174         4796 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.175, whole genome shotgun sequence.
ACCESSION   DANLYI010000174 DANLYI010000000
VERSION     DANLYI010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4796
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.175"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            141..1934
                     /gene="lepA"
                     /locus_tag="PXD81_002077"
     CDS             141..1934
                     /gene="lepA"
                     /locus_tag="PXD81_002077"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HDL5538068.1"
                     /translation="MQNIRNFSIIAHIDHGKSTLSDRLIQTCGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLNYKAKDGEIYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAIEIDLEVVPILNKIDLPAAEPERVAEEIEDIVGIDAIDAVR
                     CSAKTGLGIEDVLEEIVKKMPAPEGDAEAPLQALIIDSWFDNYLGVVSLVRVKNGVLK
                     KGDKIKVMSTGQSYNVDRLGIFTPKQVDTTELKTGEVGWVVCAIKDILGAPVGDTLTH
                     HHNPATEVLPGFKKVKPQVYAGLFPISSDDYEAFRDALGKLSLNDASLFYEPETSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVVQTSGETIYVDSPSKLPA
                     INNIAEIREPIAECNMLVPQEFLGNVITLCVEKRGVQTNMVYHGNQIALTYEIPMGEV
                     VLDFFDRLKSTSRGYASLDYGFKRFQTADMVRVDIMINGERVDALAIIVHRANAAYRG
                     RELVEKMRELIPRQQFDIAIQAAIGNHVIARSTVKQLRKNVLAKCYGGDVSRKKKLLQ
                     KQKEGKKRMKSLGNVEVPQEAFLAILHVGKD"
     gene            2021..2980
                     /gene="lepB"
                     /locus_tag="PXD81_002078"
     CDS             2021..2980
                     /gene="lepB"
                     /locus_tag="PXD81_002078"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009875129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HDL5538069.1"
                     /translation="MAQFMPIIFLGIIYGIWQWLDSMQLPNTISIILVALVIICGGFW
                     AFYKFSADPRRKQAIVLQQKRLGRELTAEEIAEIQPKSAVGEFFASLFGILFFVTVLR
                     SFLFEPFQIPSGSMEPTLRVGDFLVVNKFSYGIKDPIWQNTLIETGKPERGDVIVFKA
                     PAQPHVDYIKRVVAVGGDKIKYDFATQQLTVTKANGETKVFEYSEGKPNPEFFYHGEM
                     QVERTEKGNVTHQILNHPMAFNYAPYFYQQEGQAEGEWVVPQGYYFVMGDNRDNSEDS
                     RFWGFVPERNIVGKATFIWLSLDKKANEFPTGLRFSRMFTSIN"
     gene            3026..3700
                     /gene="rnc"
                     /locus_tag="PXD81_002079"
     CDS             3026..3700
                     /gene="rnc"
                     /locus_tag="PXD81_002079"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HDL5538070.1"
                     /translation="MQLERLQKKLGYQFHNLDYLIQALTHRSAATKNNERLEFLGDSI
                     LNFTIGKVLYDKFPKANEGELSRMRATLVREQTLAIIARKFDLGDYLKLGPGELKSGG
                     FRRESILSDCVEAIIAAIYLDQDMAKAMEKVEDWYSDLLKDISPGDSQKDPKTRLQEF
                     LQGRKLPLPEYDVLDIKGEAHNQTFRVTCKVINVEEVFIGIGTSRRKAEQNAAEKALA
                     VVKTKK"
     gene            3803..4720
                     /gene="era"
                     /locus_tag="PXD81_002080"
     CDS             3803..4720
                     /gene="era"
                     /locus_tag="PXD81_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013525392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HDL5538071.1"
                     /translation="MTEQIQTPAKTYCGFIAIVGRPNVGKSTLLNKILGQKISITSRK
                     AQTTRHRIVGIHTEDQYQAIYVDTPGLHIEEKRAINRLMNRAASSAIGDVDLIIFVVE
                     GTKWTEDDEMVLNKLRSAKAPVLLAINKIDNIKEKEELLPHITELSQKFDFAEILPIS
                     AQRGKNVHILQKFVRQSLRQGEHHFPEEYVTDRSQRFMASEIIREKLMRFTGEELPYS
                     VTVEIEQFKMNERGVYEINGLILVERDGQKKMVIGAGGQKIKTIGIEARKDMERLFDN
                     QVHLELWVKVKAGWADDERALRSLGYMDE"
BASE COUNT         1525 a          869 c         1062 g         1340 t
ORIGIN      
        1 aagaaatttg cacattttta ggaaaaaata accgcttatt gctatttgtt ttctttgcaa
       61 aaccttgtat ttcaagtaca attccccgat taatttttga taaacaaata acaaaacagg
      121 acattaactg cccgatgaaa atgcaaaata ttcgtaactt ttcgattatc gctcatatcg
      181 atcacggcaa atcgacttta tcagaccgct taattcaaac ttgtggtggc ttatcagatc
      241 gtgaaatgga ggctcaagta ttagattcaa tggatcttga gcgtgagcgt ggaattacta
      301 ttaaagcaca aagtgtaacc ttaaactata aagcgaaaga tggcgaaatt tatcagctca
      361 actttattga tacgccaggg cacgtggatt tctcatatga agtttctcgc tcgcttgctg
      421 cttgtgaagg tgctttatta gtggttgatg cagggcaggg ggtggaagca cagacgttgg
      481 cgaattgtta taccgcaatt gagatagatt tagaggttgt gccaatttta aataaaatcg
      541 atttaccagc agccgagcct gagcgtgtgg cagaagagat tgaagatatt gtcggcattg
      601 atgccattga tgcagtacgc tgttcagcta aaacaggttt aggcattgaa gatgttttag
      661 aagaaatcgt gaagaaaatg cctgcaccag aaggcgatgc ggaagcacca ttacaagcgt
      721 taattatcga ctcttggttc gataactatt taggcgttgt gtctttagtg cgtgtgaaaa
      781 atggcgtgct gaaaaaaggc gataaaatta aagtgatgag tacagggcag tcttacaatg
      841 tagatcgcct tggtattttc acgccaaaac aagtggatac taccgagcta aaaacaggtg
      901 aagtaggttg ggtagtttgt gcgattaaag atattttagg ggcaccagtg ggcgatacat
      961 taacccacca ccacaaccca gcaactgaag tattaccggg ctttaagaaa gtaaaaccac
     1021 aggtttatgc agggttattt ccgattagtt cagacgacta cgaagccttc cgtgatgctt
     1081 taggcaaatt aagcctaaat gatgcgtcat tgttctatga gccagaaact tcaagtgcgt
     1141 tgggctttgg cttccggtgt ggcttcttag gcttgttgca tatggagatc attcaagagc
     1201 gtttagagcg tgaatacgat cttgatttga ttactaccgc tccgaccgta gtttacgaag
     1261 tggtgcaaac cagtggcgaa acgatctatg tggatagccc atcgaaactc ccagcaatca
     1321 acaatattgc agaaattcgt gagccgattg cagaatgtaa tatgcttgtg ccacaagaat
     1381 tcctaggcaa cgtgattacc ctttgtgtgg aaaaacgtgg cgtacagacc aatatggtct
     1441 atcacggtaa ccaaatcgcc ttaacttacg aaatcccaat gggcgaagta gtgttagact
     1501 tcttcgaccg cttaaaatca acctctcgtg gttatgcttc actcgattac ggttttaaac
     1561 gtttccaaac ggctgatatg gtgcgtgtgg atattatgat taacggcgag cgtgttgatg
     1621 cgttggcgat tatcgttcac cgtgcaaatg cggcgtatcg tggacgtgaa ttagtggaaa
     1681 aaatgcgtga acttattcca cgccaacaat ttgacatcgc tattcaagcg gcaattggta
     1741 accacgtcat tgctcgctca acggtaaaac agttgcgtaa aaacgtatta gcaaaatgtt
     1801 acggtggtga tgtaagtcgt aagaagaaac tgctacaaaa acaaaaagag ggtaaaaaac
     1861 gaatgaaatc tctcggtaac gtagaagtgc cacaagaagc atttttggca attcttcatg
     1921 tgggtaagga ttaattgtca atattcgtgt aaaatacgac cgcttgcaag cggtcatatt
     1981 ttgagaaaac tttgcaattt tgaataaaaa ggagcattca gtggcacaat ttatgccaat
     2041 tatttttcta ggtattattt atggtatttg gcaatggctt gattcaatgc aactgccaaa
     2101 tactatttcc attattttag ttgccttggt tattatttgt ggtggatttt gggcgtttta
     2161 caaattcagt gctgacccaa gacgtaaaca ggcgattgtt ctacaacaaa aacgtttagg
     2221 cagagaatta accgctgaag aaatagcgga aattcaacct aaatccgcag taggcgaatt
     2281 ttttgcctca cttttcggca ttttattctt tgttacagta cttcgctcgt tcttatttga
     2341 accgtttcaa attccaagtg gttcaatgga gccaaccttg cgtgtaggcg attttttagt
     2401 cgtaaataaa ttcagctacg gcattaaaga tcctatctgg caaaacacct tgattgaaac
     2461 gggcaaacca gaacgaggcg atgtgattgt attcaaagca ccggcacaac cacacgttga
     2521 ttatattaag cgtgtggtgg cagtaggtgg tgataaaatt aaatatgatt ttgcaactca
     2581 gcaattaacg gttactaaag ccaatggcga aacaaaggta tttgaataca gcgaaggcaa
     2641 gccaaatcct gagtttttct atcacggtga aatgcaggta gaacgcactg aaaaaggtaa
     2701 tgtaactcat caaattttaa atcacccaat ggcatttaac tatgcacctt atttctacca
     2761 acaagaaggg caagcagaag gtgaatgggt tgtgccacaa ggatactatt ttgtgatggg
     2821 tgataaccgt gataacagtg aagacagtcg tttctggggc tttgtgcctg agagaaacat
     2881 cgttggtaaa gccactttta tttggttaag tttagataaa aaagcaaatg aattcccaac
     2941 ggggcttcgt ttttctagaa tgtttacctc aatcaattaa tcttaacagg ggcgtatgag
     3001 atacgcccct ttattcacat aaactatgca actagaacga ttacagaaaa aattaggtta
     3061 tcaatttcat aaccttgatt acctcattca ggccttaaca caccgcagtg cggcaaccaa
     3121 aaacaatgag cgtcttgaat ttctaggcga ttcaatttta aattttacta ttggtaaagt
     3181 attatacgac aaatttccaa aagcgaatga aggtgagctt agccgtatga gagcaactct
     3241 tgtgcgtgag caaactctag cgattattgc tcgcaaattt gatttaggcg actacctaaa
     3301 attaggtcca ggagagctaa aaagtggtgg ttttcgccga gaatcgattt tatcggactg
     3361 tgttgaagcg attattgcgg ccatttatct cgatcaagat atggcaaaag caatggaaaa
     3421 ggtggaagat tggtattcag atttgcttaa agatatcagt ccaggcgatt cacaaaaaga
     3481 tccaaaaacc cgcctgcaag agtttttaca aggtagaaaa ttgccattgc ctgaatatga
     3541 tgtattagat attaaaggcg aagctcataa ccaaactttc cgtgtaacct gtaaagtgat
     3601 taacgtcgag gaagtgttta tcggcattgg caccagccgc cgaaaagccg aacaaaatgc
     3661 cgcagaaaaa gcgttagcgg tggttaaaac gaaaaagtaa gatagcgcaa gcgtcctcgc
     3721 ttgtgctaaa gatttaatta aatggtacaa gcgtggacgc ttgcaccatc aaattcattc
     3781 cacagaatga aaggaaaaca aaatgacaga acaaattcaa actcctgcaa aaacctattg
     3841 tggttttatt gcgattgttg gtcgcccaaa tgtagggaaa tcaacattat taaataagat
     3901 tttaggacaa aaaatttcca ttacctcacg caaagctcaa acgactcgcc atcgtattgt
     3961 tggtattcac actgaagatc aataccaagc gatttatgta gataccccag gcttacacat
     4021 agaagaaaaa cgtgcgatta atcgtttaat gaaccgtgca gcaagttctg ctattggcga
     4081 tgtagatctc atcatctttg ttgtggaagg cacaaaatgg acagaagatg atgaaatggt
     4141 gttaaacaaa ttgcgttcgg caaaagcccc tgttttattg gcaatcaata aaattgataa
     4201 catcaaggaa aaagaagaac ttctgccgca tattaccgag ctttcacaaa aatttgattt
     4261 tgctgaaatt ctgccaattt cggcacaacg gggtaaaaat gtgcatattt tacagaaatt
     4321 tgtccgtcaa tcactgcgtc aaggtgagca ccactttcct gaagagtatg tgacagaccg
     4381 ctctcaacgc tttatggcat cagaaattat tcgtgaaaaa ctaatgcgtt ttacaggtga
     4441 agagttacct tattcggtaa cggtagaaat cgaacaattt aaaatgaatg agcgtggggt
     4501 gtatgaaatc aatggcttga ttttagtaga acgagatggt cagaaaaaaa tggttattgg
     4561 tgcggggggg caaaaaatca aaaccatcgg cattgaagct cgaaaagata tggaacgcct
     4621 atttgacaac caagtgcacc ttgagctttg ggtgaaagta aaagctggtt gggcggatga
     4681 tgaaagagca ttgagaagtt tagggtatat ggacgagtag ttggctcccg ttatagcatt
     4741 aatatcgtaa tcaaatctcc cctaacccct cttttctaaa gagggggact agttct
//
LOCUS       DANLYI010000175         4786 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.176, whole genome shotgun sequence.
ACCESSION   DANLYI010000175 DANLYI010000000
VERSION     DANLYI010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4786
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.176"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            129..2765
                     /locus_tag="PXD81_002081"
     CDS             129..2765
                     /locus_tag="PXD81_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HDL5538072.1"
                     /translation="MDEGFMVKHPRHSSHLHPLIHQLTTPEGEVRIYDASYPQFEVNP
                     VEKEDGIDLIPSLETHGAGVAGIIAAQADKTLGDGYSGGIAKGAELYVATKSYKRTLE
                     KVIQDAKKELENAKDEEDEKTPSLDQMAKNDLLASKEKEMAIERAEWASGLNKLLDNN
                     VFAINNSWNPFSISDDINVVDKFYQSIKQNKHNPLLQAIMRAKNSNTLLVFAAGNESK
                     KQPGVMALLPRYFPELEKNLISAVAVDKEQKIASYSNHCGASKNWCVAAPGDLHVLIG
                     VADEHKKPQYGLTKEQGTSFSAPAITASLAVLKERFDYLTATQIRDTLLTTATDLGEK
                     GVDNVYGWGLINLKKAVNGPTQFLNDETITVTRDDHWSNPLASQFKITKKGDKSLHLD
                     GENHLDTVAVEEGRLALNGKTKVKTISNHANLAVNGTEVEQNYSSSGQSQLEVLGKSG
                     LIANAQANIHLAGSLKIDDKLTEKTEAGDVSATVVQLKDKATYQGGFTQLVENENLAK
                     RGLIQDLYFKESEIIAKVNKPLTDEKADTNGQAGLALLNALRTTPIAYRRSWYNGWLQ
                     SALEQRKLDNLHYAVSNNIYADSLELLRSQNAKGLTQAQQHLFTAYHTPLQTTVWAEH
                     LNQKQSASSKHTDVKHHQSQLGVNHKLADKTVLSATLSQQKNRLEKPFAQATLKQTAL
                     NIGLRYHLDNAWFSEATLQFARQKYQQSRRFASHQLGTAETRGSTLGGEMRIGYQFMP
                     NQWIIEPSLGVQWIQTKMNGLNESGELATQTAAMRYRDVNIVPSVKLQRTFQLEQGSI
                     SPYIGLNYLHRLNGKITKITSNIAGKTLHSEATTKRNRQLNGEVGVKLHYKNWFTAMN
                     LDYSRVKSANLFGWKVNVGFSF"
     gene            complement(2906..3343)
                     /locus_tag="PXD81_002082"
     CDS             complement(2906..3343)
                     /locus_tag="PXD81_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HDL5538073.1"
                     /translation="MDWFFNWIGWSILGFVLLALELVLPGVFIMWWGFASLILAFIVA
                     LFTLGQALQIALFAIIATIFSLIWWKYQHGKDKKEDQSSSLNSREHAMLGAQGFVVEI
                     LPNGIARGKFGDTTWRVIGNNLQVADKIEVQKVEGITLFVRKQ"
     gene            complement(3379..4299)
                     /locus_tag="PXD81_002083"
     CDS             complement(3379..4299)
                     /locus_tag="PXD81_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HDL5538074.1"
                     /translation="MNFDLPIVSIAFVVLVLVALSSTIKIVPQGYHWTVERFGRYTKT
                     LSPGLNIVVPFIDRIGRKMNMMEQVLDIPSQEVISRDNASVAIDAVCFVQTVDARRAA
                     YEVNHLEQAIVNLTMTNMRTVLGSMDLDDMLSQRDLINGRLLSIVDEATNIWGVKVTR
                     IEIRDVRPPKELVAAMNAQMKAERNKRADILEAEGIRQAEILRAEGEKQSRILKAEGE
                     RQEAFLQAEARERAAEAEAKATQMVSEAIAKGDTTAINYFIAQKYTEALKEIGSSDNS
                     KVVLMPLEAGNLIGSVAGIAEILKSNKTSS"
     gene            complement(4315..>4786)
                     /gene="ung"
                     /locus_tag="PXD81_002084"
     CDS             complement(4315..>4786)
                     /gene="ung"
                     /locus_tag="PXD81_002084"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HDL5538075.1"
                     /translation="FSVKPPVAPPPSLVNMYKELSEDIEGFQIPPHGYLIDWAKQGVF
                     MLNTSLTVEQGRAGSHSQIGWEIFTDKVIAQLNQHRDNLVFLLWGSHAQRKGQFIDRN
                     RHCVLASPHPSPLSAYRGFFGCKHFSKANNYLISQGISPINWQLSVDVSYSSTH"
BASE COUNT         1601 a          974 c          892 g         1319 t
ORIGIN      
        1 caattagcat ttctagtttt ttatctattg catacgctac agaatcaata gagaatccac
       61 aacctatcat tcaattatct gagagcctca gctcaaaata tagtggcaag ggtgttaaac
      121 taggcgtaat ggatgagggc ttcatggtta aacaccctcg ccatagttca catcttcatc
      181 ctcttattca tcaattaaca acacccgaag gcgaagtaag aatctacgat gcaagttacc
      241 ctcaattcga ggtaaacccc gtagaaaaag aagatggaat tgatcttatt ccttctcttg
      301 aaacacacgg tgcaggtgta gctggtatta ttgccgctca agcagataaa actcttggtg
      361 atggctatag tggtggtatt gcaaagggag ctgagcttta tgttgcaacg aagtcatata
      421 aacgaacttt agaaaaagtt attcaagatg ctaagaaaga attagaaaat gcaaaagacg
      481 aagaggatga aaaaacgcca agtctggatc aaatggcaaa gaatgactta ttagcaagca
      541 aagaaaaaga aatggcaatt gaacgtgctg aatgggcatc cgggctgaat aaattactag
      601 ataataatgt ttttgcaatc aataatagtt ggaatccttt ttctattagc gatgacatca
      661 atgttgtaga taaattttat caatctatca agcaaaataa gcacaatccg ctacttcaag
      721 ctattatgag agccaaaaat agcaacacct tattagtctt tgctgctggt aatgagagca
      781 agaaacagcc aggcgtaatg gctcttctac cacgttactt tcccgagctt gagaaaaatt
      841 taatttctgc cgttgcggta gataaagaac aaaaaattgc ttcatactct aaccactgcg
      901 gagcaagtaa aaactggtgt gtagcagcac cgggcgattt acatgtttta attggcgttg
      961 ctgatgaaca taaaaaacca caatatggct taacaaaaga gcaaggtacg tcattttcag
     1021 cacctgctat aactgcttca ttagcagtat taaaagaacg ctttgattat ctgactgcga
     1081 ctcaaattcg cgatacttta ttgactaccg ccactgattt aggtgaaaaa ggggtcgata
     1141 atgtttatgg ctggggtctc attaacctga aaaaagctgt taatggccca actcaattcc
     1201 taaatgatga aacgattacc gtaactcgtg atgaccattg gtcgaatcca ctcgccagcc
     1261 aatttaaaat tacaaaaaaa ggggacaaat cactgcattt agacggcgaa aatcatttag
     1321 atacggtagc agttgaagaa ggtagattgg ccttaaacgg taaaactaag gtgaaaacaa
     1381 tcagtaacca tgcaaattta gctgtcaatg gaaccgaagt tgagcaaaat tattcttcgt
     1441 ctggacaaag ccaattagaa gtgttgggca aatctgggct aattgccaat gctcaagcca
     1501 atatacattt agccggctcg ttaaaaattg atgataaatt aacagaaaaa accgaagcag
     1561 gtgatgtttc agcgaccgta gtacagttga aagataaagc aacttatcaa ggcggattta
     1621 cacaattagt cgaaaacgag aatttagcca aacgagggtt aatccaagat ctctatttca
     1681 aagagagcga aattatcgca aaagtgaata agccactaac tgatgagaaa gccgatacaa
     1741 acgggcaagc cggtttagca ttattaaatg cgttaagaac tacacctatt gcctatcgcc
     1801 gtagttggta taacggttgg ctgcaatctg cactcgaaca acgtaaatta gataatctgc
     1861 actatgctgt atctaacaat atttatgcag atagccttga attgctacgt agccaaaatg
     1921 ccaaaggctt aacccaagca cagcaacatt tgtttacggc ataccataca ccactgcaaa
     1981 cgactgtttg ggctgaacat ttgaatcaaa aacaatcagc ctcatcgaag cataccgatg
     2041 ttaaacacca ccaatcacaa ttgggagtaa accacaaact ggcagataaa acggtactta
     2101 gtgcaacact ttcacagcaa aaaaaccgct tagaaaaacc atttgcacaa gctaccttaa
     2161 aacaaacagc attaaatatt ggcttacgtt atcatttaga taacgcttgg tttagtgaag
     2221 ctacattgca atttgctcgc cagaaatatc aacaaagccg tcgatttgct agccatcaac
     2281 taggtactgc agaaaccaga ggttcaacgc tcggtggtga aatgcggatc ggttatcaat
     2341 ttatgccaaa ccaatggata attgaaccaa gccttggcgt acaatggatt caaaccaaaa
     2401 tgaatggttt aaatgaaagt ggcgaacttg cgactcaaac ggctgcaatg cgttatcgtg
     2461 atgtgaatat tgttccaagc gtgaaattac agcgaacttt ccaacttgag caaggctcta
     2521 tttcccctta tatcgggcta aattacttgc atagattaaa tggaaaaata accaaaatta
     2581 ccagtaacat tgcaggaaaa acactgcata gtgaagcaac gacgaaacgt aatcgccaat
     2641 taaacggcga agtcggggtg aaactgcatt ataaaaattg gtttactgcg atgaatttag
     2701 attacagtcg agtgaaatct gcaaacctat ttggttggaa agtaaatgtt ggctttagtt
     2761 tctaacttat tatggtggga cacaagcggt taatttaggg tgaaaatttg caaatttcac
     2821 tctattttca accgcttgag agaaaaaacc ttgaacaact gttcaaggtt tttgtttagt
     2881 gttctatgac taaataagaa aaaacttatt gcttacggac aaataaggta attccctcca
     2941 ctttctggac ttcaatctta tctgctactt gtaaattatt acctataaca cgccaagtcg
     3001 tatcaccaaa ttttcctcga gcgatgccat taggtaagat ttcaaccaca aagccttgag
     3061 ctcctagcat agcatgctct cgagagttaa gggaactgct ctgatcctct ttcttgtctt
     3121 ttccatgctg atatttccac caaataagac taaaaatagt cgcaattatc gcaaataacg
     3181 caatttgtaa cgcttgccct aatgtaaaaa gtgcaacgat gaaagcaaga ataagcgagg
     3241 caaatcccca ccacataata aagacacccg gtaatactaa ttctaatgca agtagtacaa
     3301 agcctaatat gctccagcca atccaattaa aaaaccagtc catactccct ccatatactc
     3361 aattaatccg gttttaggct atgagcttgt tttattgctt tttaaaattt cagcaatacc
     3421 agcaacagaa ccaatcaaat ttccagcttc taacggcata agtacaactt tactgttatc
     3481 tgaagaacct atttctttca aagcttcggt gtatttttgt gcaataaaat agttaatcgc
     3541 tgtagtatcg cctttagcaa ttgcttcaga aaccatttga gtggctttag cttccgcttc
     3601 tgcggctcgt tcacgagctt ctgcctgtaa gaatgcttct tgtctttcac cttctgcttt
     3661 taaaatacga gattgttttt ccccttcagc ccgcagaatt tcagcttgtc gaatcccttc
     3721 tgcttcaaga atatcagcac gtttattacg ctcagctttc atttgagcgt tcattgccgc
     3781 aaccagctct ttcggtggac gaacatcccg aatctcaata cgggtgactt ttactcccca
     3841 aatattagtc gcctcatcga caatggaaag taatctaccg ttaattaaat cacgctgaga
     3901 gagcatatca tctagatcca tagagccgag cacggtacgc atatttgtca tggttaaatt
     3961 aacaattgct tgctctaaat gattcacttc ataggcagct cggcgagcat caactgtttg
     4021 aacaaaacat acagcatcaa tcgcaacact tgcattatca cgtgaaataa cctcttgcga
     4081 tggaatatct aaaacttgct ccatcatatt catttttcgc ccaatacgat caataaaagg
     4141 tacgacaatg ttcaaccccg ggcttagcgt tttagtataa cgaccaaaac gttctactgt
     4201 ccaatgatag ccctgtggca caattttaat agtcgaactt aatgcaacta aaacaagcac
     4261 aacaaatgca atactaacaa tcggtaaatc aaaattcatc gtttgctcct taaactaatg
     4321 tgttgaagaa taactaacgt ctactgataa ttgccaatta attggggata ttccttgaga
     4381 aatcaagtag ttattggctt tagaaaaatg tttacaacca aaaaatccac gatatgcgga
     4441 aagtggtgat gggtgcggag aagctaacac acaatggcga ttacgatcga taaattgccc
     4501 ctttctctga gcgtggcttc cccaaagtag aaagactaaa ttatcacggt gttgatttaa
     4561 ttgtgcgatc actttatcgg taaaaatttc ccaaccaatt tgtgaatgcg aaccggctct
     4621 accttgctca accgttaagc tggtgtttaa cataaatacc ccttgttttg cccagtcaat
     4681 taaatagcca tgcggtggaa tttgaaatcc ttcaatatct tcagataatt ctttatacat
     4741 attgaccaga gaaggtggcg gggcaaccgg cggtttaact gaaaag
//
LOCUS       DANLYI010000176         4695 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.177, whole genome shotgun sequence.
ACCESSION   DANLYI010000176 DANLYI010000000
VERSION     DANLYI010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4695
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.177"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            112..1302
                     /locus_tag="PXD81_002085"
     CDS             112..1302
                     /locus_tag="PXD81_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000462687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/tyrosine/aromatic aminotransferase"
                     /protein_id="HDL5538076.1"
                     /translation="MFSHIQAAPADSILGLGEAFKADSREGKINLGIGVYMTDEGKTP
                     IVKAVKEAEKRLLETENSKNYLTIDGVQAFNAATQALLFGEGAEVITSGRAKTAQSLG
                     GTGALRIAAEFIKRHTNAKNVWISTPTWPNHNGIFDAVGVNVKGYRYYNKETNGLDWE
                     NLIADLSQAEAGDVVLLHGCCHNPTGIDPTPAQWEQLAALSAEKGWLPLFDFAYQGFA
                     NGLEEDAYGLRAFVKNNRELLVASSFSKNFGLYNERVGAFTLIADNADDANRAFTQIK
                     SIIRVLYSNPSAHGASAVAVALADPDLKAQWIAELDEMRNRIKEMRSQLVQKLKEKGA
                     NKDFSFITEQNGMFSFSGLTPEQVDKLKDEFAIYAVRSGRINVAGITSKNIDALADAI
                     VKVL"
     gene            complement(1409..2710)
                     /gene="folC"
                     /locus_tag="PXD81_002086"
     CDS             complement(1409..2710)
                     /gene="folC"
                     /locus_tag="PXD81_002086"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HDL5538077.1"
                     /translation="MNTLSIPKATDSLETWLSYLEQAHSKPIDMGLDRIRKVATELDL
                     LKPAPYVITVAGTNGKGSTCRLLEVTLLKAGFRVGVYSSPHLIHYNERVRINGEVLPD
                     ADHVQAFAKINAKKTASLTYFEFSTLSAFELFKQNQLDIVILEVGLGGRLDATNIIDP
                     SLAVIISIDIDHVAFLGDNREDIGREKAGIFRENIPVVIGEPDCPHSIKQIADSLNCH
                     AFYRGKDWQFEIKNNLFHWYSEEKSFTDLPIPLIPIPNAGTALAVLMQLPFEIDEKII
                     RQALNEAQMTGRFQTLTDTDFTHFSQPKPAAKVIIDVGHNPHAARYLADKLRELKQPN
                     QKIYAVFSALEDKDLSGIVEPLANIIDEWHCAGLDCWRGQTGEAVLAKLVNVLPNSTA
                     CSYENVAQASRVLFETANENDIVLVFGSFHTVADFMLWLET"
     gene            complement(2720..3616)
                     /gene="accD"
                     /locus_tag="PXD81_002087"
     CDS             complement(2720..3616)
                     /gene="accD"
                     /locus_tag="PXD81_002087"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HDL5538078.1"
                     /translation="MSWLDRILGRDSSSSSNAKSKVPEGVWTKCTSCEQVLYSEELQR
                     NQQVCPKCDHHMRIDARTRLLNLLDKDSAVEIAADLEPEDVLKFKDLKKYKDRLTAAQ
                     KQTGEKDSFIVLAGTLHNMPVVTASFNFEFMGGSMGSVVGAKFVKAAEKALAEKIPFI
                     CFSASGGARMQEALFSLMQMAKTSAILAKMKEQGVPFISVLTDPTLGGVSASLAMLGD
                     VNIAEPKALIGFAGPRVIEQTVREKLPEGFQRAEFLLEHGAVDMIVHRKEMRDTLSRL
                     CAKLTNQPTPFKVAELVVENAE"
     gene            3925..4695
                     /gene="mazG"
                     /locus_tag="PXD81_002088"
                     /pseudo
     CDS             3925..4695
                     /gene="mazG"
                     /locus_tag="PXD81_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005600778.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
BASE COUNT         1416 a          957 c          962 g         1360 t
ORIGIN      
        1 taatttatag gttttattac atttttttgt gtttttgctc ttgctaaatc tgcaatttaa
       61 tcgtataaat tcactgttta tttttcattt tgatgaggaa ctccaatgtc aatgtttagt
      121 cacattcaag ctgctccggc agactcaatt ttaggcttag gtgaagcgtt taaagcagat
      181 agccgtgaag gtaaaatcaa ccttggtatc ggtgtttata tgaccgatga aggcaaaacc
      241 ccgattgtca aagcggtaaa agaagcagaa aaacgtttat tagagacgga aaacagtaaa
      301 aattatttaa ccattgacgg tgttcaagca ttcaatgcgg caacgcaagc gttattattt
      361 ggcgaagggg ctgaagtgat tacctcaggt cgtgctaaaa ctgcacagag tttaggtggg
      421 acaggggcat tacgcattgc ggcggaattt atcaaacgtc atacgaatgc gaaaaatgta
      481 tggatttcaa ccccgacttg gccaaaccat aacggcattt ttgatgcagt aggcgtgaat
      541 gtaaaaggct atcgctacta caataaagag actaacggtt tagattggga aaatttaatt
      601 gccgatttaa gccaagcgga agccggagat gtggtgttat tgcacggttg ctgccacaac
      661 ccgaccggaa ttgacccaac acctgcacaa tgggaacagt tagcagcatt atctgccgaa
      721 aaaggctggt tgccgttatt tgactttgct tatcaaggct ttgcaaacgg tttagaggaa
      781 gatgcttacg gtttacgagc ttttgtgaaa aataaccgtg aattgttggt cgcaagctca
      841 ttctcgaaaa actttgggtt atataacgag cgtgtcggtg catttaccct aattgcagat
      901 aatgctgatg atgccaaccg tgctttcact caaatcaaat cgattattcg tgtactttac
      961 tctaacccat ctgctcacgg tgcaagtgca gtagcagttg cattggctga tcctgacctt
     1021 aaagcacaat ggattgcgga attagatgaa atgcgtaacc gtattaaaga aatgcgtagc
     1081 caattagtgc agaaattaaa agaaaaaggg gcaaacaaag acttctcttt cattactgag
     1141 caaaatggta tgttctcatt cagtggctta acaccggagc aagtggataa gttaaaagat
     1201 gagtttgcaa tttatgctgt gcgttcaggc agaatcaatg tagcaggcat taccagcaaa
     1261 aacattgatg ctcttgccga tgcgattgtg aaagtgcttt aattaaacct taaagatata
     1321 tagaggcata agcctgagtg cttgtgcctc ttttttatct tttaggtaca agtgtggaca
     1381 cttgcaccaa cgttgattat tcgtagaact aggtttccaa ccaaagcata aaatctgcta
     1441 ccgtatgaaa tgagccgaac accagcacaa tatcattttc gttggctgtt tcaaacaata
     1501 ctcggcttgc ctgtgccaca ttttcatagc tacaagcggt tgaatttggc aaaacgttta
     1561 ccaatttcgc taacaccgcc tcgcctgttt gtccacgcca acaatctagc cctgcacaat
     1621 gccattcatc aataatatta gctaatggct cgacaatacc ggataaatcc ttatcttcta
     1681 acgcactaaa caccgcatag attttttgat ttggttgctt taactcccgc agtttgtcag
     1741 ctaaataacg tgctgcgtgt gggttatgcc ctacatcaat aattactttt gcagccggtt
     1801 taggttgaga aaaatgagta aaatcggtat ccgtcagggt ttgaaaacgc cctgtcattt
     1861 gagcttcatt taaggcttgt cgaataatct tttcatcaat ttcaaatggc agttgcatta
     1921 atactgctaa tgcggtgccg gcgtttggaa tcggaataag cgggattggt aaatcggtaa
     1981 aggatttttc ttcagaatac caatgaaaca gattattctt aatttcaaac tgccagtctt
     2041 taccccggta aaaagcgtga caatttaacg aatcagcaat ctgtttaatc gaatgagggc
     2101 aatcaggctc gccgatgaca accggaatat tttcacggaa aatgccggct ttctcacgcc
     2161 cgatgtcttc acggttatcg cctaaaaatg caacgtggtc gatatcaatc gagataatca
     2221 cggcaaggct tggatcaata atattagtgg catctaatcg cccacctaat ccgacttcta
     2281 aaatcacaat atcgagctga ttttgtttga atagctcaaa tgcagacagc gtactaaact
     2341 caaaataagt gagtgaagcg gtcttttttg cattaatttt tgcaaatgct tggacgtggt
     2401 cggcatcagg taatacttcg ccgttaattc gcacccgttc attatagtga attaagtgcg
     2461 gtgaagagta aacaccaacc cgaaatccgg cttttaataa ggtaacttct aacaaacggc
     2521 aggtcgaacc cttgccgtta gtaccggcaa cggtaatcac ataaggggca ggcttgagta
     2581 aatccagctc tgttgcgacc ttacgaatac gatctaagcc catatcaatc ggtttagaat
     2641 gggcttgttc taaataggaa agccacgttt ccaaagaatc cgtggcttta ggtatgctta
     2701 atgtattcat tcagatattt tattctgcgt tttctacaac caattccgcc actttaaacg
     2761 gcgttggttg attggttaat tttgcacaaa gacgggaaag ggtatcacgc atctctttac
     2821 gatgcacgat catatcaact gccccgtgtt ctaataagaa ctcggcacgt tggaaacctt
     2881 ccggcagttt ttcacgtacg gtttgctcaa ttacacgagg accagcaaag ccgattaggg
     2941 cttttggctc agcaatattc acatcgccca gcattgccaa acttgcggaa accccaccta
     3001 aagttgggtc ggttaataca gaaataaacg gtacgccctg ctctttcatt ttggctaaaa
     3061 tcgcactggt ttttgccatt tgcattaaag agaataacgc ctcttgcata cgagccccgc
     3121 ccgatgccga aaaacagata aacggaattt tttcagctaa agctttttcc gcagctttca
     3181 caaattttgc acctactaca gaccccattg aaccgcccat aaactcaaag ttaaaggagg
     3241 ccgtaaccac cggcatatta tgcaatgtac cggcaagcac gataaaagaa tctttctcac
     3301 cggtttgttt ttgcgcggca gttaaacgat ctttatattt ctttaaatct ttaaatttca
     3361 acacatcttc cggctctaag tcggcagcga tttcgaccgc agaatcttta tctaacaaat
     3421 tcaataaacg ggtgcgggca tcaatacgca tatgatgatc gcattttgga caaacctgtt
     3481 ggttgcgttg taattcttcg ctatagagaa cttgttcaca acttgtgcat tttgtccata
     3541 caccttccgg cacttttgat tttgcattgc ttgatgatga agagtctcta cctaaaattc
     3601 tgtctaacca gctcattatt attccttttg gattgagttg ttgggttcac taaatggttg
     3661 ctattaaagc ataatttatc ggtttttgat atgttttctt gacatatcag atcactaact
     3721 atgtgtataa gttcgataaa aaaacgcaat cagaagaatg attaacatca ccacaatttg
     3781 aattaagcgt tttttggtta atttttccgc tgccattgct ctgctcatac ttaataaatt
     3841 tccgcattat aagcgaaaaa gcacgggcta tgtagaattt gcaaaatttt gatcaaaaat
     3901 aaccgcttgt aaaaaggaaa tactatggca accattgaac aatttcttga ggttgtaaac
     3961 caacttcgtc accctgaaca cggctgcccg tgggatctca aacagaattt tgacacgatg
     4021 ttccccactt attggaagaa acctacgaag tagcggaagc gattcacacc caagatcgca
     4081 ctgccttgcg tgaagagtta ggcgatttat tactgcaagt ggtattttta agccaattag
     4141 cgaaagagga aggcagtttc acttttgatg atgtagtaac ggatattcac gataaactga
     4201 tttaccgcca ccctcacgtt tttggtgatg tgaaagctac taacagcgaa gaggccttac
     4261 aaagttggga agcacaaaaa gcgaatgatg caaaacaccg tagccaagat tctattttta
     4321 gatgatttac cttttgcttt gcctgctctt acccgtgcaa ataagctaca aaaacggtgt
     4381 gcaaaagttg gattcgattg ggataaccct cacgatgtgc tggcaaaagt cgaagaagaa
     4441 ctcgatgaag tcaaagcaga aatcgcacaa tatccggctc gctccatcga gcttgctgaa
     4501 gaattaggcg atctcttgtt tgcgattgta aatttatgcc gccattatca taccgatgcc
     4561 gaggaaattt tacgcaatgc aaatctaaaa tttgaacatc gctttcgaaa agttgaaaaa
     4621 tctgtgaaaa aatcgggaag aactgtaaaa gaatgtaaat tagaagaact agatgtaatt
     4681 tggcacaaaa taaaa
//
LOCUS       DANLYI010000177         4608 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.178, whole genome shotgun sequence.
ACCESSION   DANLYI010000177 DANLYI010000000
VERSION     DANLYI010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4608
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.178"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            complement(211..1299)
                     /locus_tag="PXD81_002089"
     CDS             complement(211..1299)
                     /locus_tag="PXD81_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDL5538079.1"
                     /translation="MGIMSKSISIFNHKGGIAKTTTAFNVGWSLANQGYQVLLVDLDS
                     QCNLTGLVLGYDQCKEDSDLALFYNNRHHLTMEGIVEALINGQSPDNYLSGNVGQITQ
                     TLNPNLFLLPGHLNVSELDSQISVSLKIAAGVPATRNIPGNLPTLIKKVAEQNNIDYI
                     LYDLSPNVGGLNEVILMSSDYFIVPTSPDFFCWQAVGSLAKNIRKWHNELKAFKELNG
                     FNQSSYAIKNKPQFLGTIQQRYRPRKGQPAKSFEKWIERIRNEVITNFVPVLKTIDCA
                     IDDTAIYAALKSTNSDLEPFDLAHIADFNSLIAISQELRVPVFSLTKEQIKNSGQFGH
                     ALNTMDESKENFDQEFQSLAERIIQLTN"
     gene            complement(1364..1795)
                     /locus_tag="PXD81_002090"
     CDS             complement(1364..1795)
                     /locus_tag="PXD81_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5538080.1"
                     /translation="MKKMAIVALSAFFSMNAFANNNVVYSCTTTDNQTLKVTKEGGNY
                     VYSHGNTTFKNPVKEALKNPASEIAGGSQFTTISLELRNAGKSYIVGHIEADPKSPFE
                     ASVQIQDIKTGNDITSFECRSDKPIRHNFDRKLMRKSGFAA"
     gene            complement(1799..3901)
                     /locus_tag="PXD81_002091"
     CDS             complement(1799..3901)
                     /locus_tag="PXD81_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrating conjugative element protein"
                     /protein_id="HDL5538081.1"
                     /translation="MKKAKSLKFLTACIMLTTMSGYANSNTVNVGNSISNFIENTTGY
                     RTGNLGVSGNNALTEYYPVLSDYMHYSIGGGSAISAPASRGRPNQYGIGIGWKSNLTC
                     GNFDIKLSIKNQLNGITEGFKDLYGNIIESATGAVASLPAMIIQRANPQLYDILSNGL
                     YQGKLDFNNFKTSCEAMSNQLADLAMDSKLAKMAGLHRAAEIAETEPDAMAAKKKMDK
                     KDAEKGIPWVGGEQRGGKNQKPMAIVADIVGAGYNLALGRGPLEKGDIPIASCDGMLC
                     TEWKNPSEAAAYASEVIGETFLDACETCEQSSSGAKAGKGIAHFIEQTTIEKATKLEA
                     ALNHLDSLTPEQLNSLSTSTVSVTKGVLEAIAASPDAALLGARLSQELAISKEMEKLL
                     LIRRAMLAGMREPNVIKSSLVEEAERNLKLLDREIEQVKLEMELQRSINGNTALAAIT
                     QQMNEQALAIDDNAMGDGNRRFANLSNQAGANNLNTSGIANSAQNAVGDRYIAIPKSD
                     GSGMSDFYGAYKASPTNIVSNVNVGSYTQIAQMNGNANEQATALIKQFEGYLSHGKWD
                     VNANRAGYGSDTYTTADGMVHRVQKGTYVSREDAERDLARRMPQFMNVARGNVGADVW
                     AKLTPQAQAALTSVSYNYGSLSKLESVQKAAAVSAQSGDMTALANSIRNLQGHNNGVN
                     ARRRNQEADYIINAPKGN"
     gene            complement(3928..4206)
                     /locus_tag="PXD81_002092"
     CDS             complement(3928..4206)
                     /locus_tag="PXD81_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDL5538082.1"
                     /translation="MQAKTFYYLPLLLLSAMASASPANKPSDVTVKVFTSESYPKVFP
                     HSGDWATHSDSFSNTENYSWALWRPYSCCKKQGQVFLGHVDWKSTHTH"
BASE COUNT         1295 a          901 c          884 g         1528 t
ORIGIN      
        1 atactatatt gcagtgtctg cattttgctg aagagaaggg agctataccg cctatacctg
       61 aagattggcg gtatgaaatg acggggcgta gtatggggat tgagcagatt ccaaatcaga
      121 aattatagct gggcatttac aaaatacaca aaagaaagta tggtgtggct aaattttagc
      181 taaccatact tttttattat gaataattgt ttaattagtc agttgtataa ttcgttctgc
      241 taatgattga aattcttggt caaaattttc tttactttca tccattgtat tcaaagcatg
      301 tccaaattga cctgagtttt taatttgctc ttttgttaag gaaaatacgg gaactcgtag
      361 ctcttggcta atagcaatca acgaattaaa gtcggctatg tgagccaaat caaatggctc
      421 taagtcagaa ttcgttgatt taagagcagc ataaatagct gtatcatcaa tagcacaatc
      481 aatagttttt aataccggca caaaattcgt aatgacttca tttctaattc gctcaatcca
      541 tttttcaaat gatttggccg gttgcccttt tcttggtcga taacgttgct gaatagtacc
      601 taaaaattgt ggcttatttt taattgcata agatgattgg ttaaaaccat taagttcttt
      661 aaatgctttt aactcattat gccatttgcg aatattttta gcgagcgaac caactgcttg
      721 ccagcagaaa aaatcaggtg atgtaggtac aataaagtaa tcactagaca ttaggataac
      781 ttcatttaat ccacccacat ttggactaag atcatagagt atgtaatcaa tattgttttg
      841 ttcagctact ttcttaatta aagtcggaag attgccgggt atatttcttg ttgctggaac
      901 accggcagca atttttaatg atacactaat ttgcgaatca agctcggaaa cattaaggtg
      961 accaggtaac aagaacagat ttggattcag ggtttgagtg atttggccaa catttccact
     1021 aaggtagtta tcagggcttt gtccgtttat cagtgcttca acaatacctt ccatagtcaa
     1081 atgatgtcta ttattgtaaa aaagcgctaa atcactatct tctttgcatt gatcgtagcc
     1141 aagtaccaga ccggttaggt tacattgtga atccaaatcg actaacaata cttgatagcc
     1201 ttgatttgct aaactccaac caacattaaa tgcagttgta gttttagcaa tacctccttt
     1261 atgattaaaa atactgatag acttactcat aatacccata attctatgaa ataattaacc
     1321 caccgaactc atttgagaat ggtgggttaa tgctttgctt tagctaggct gcaaatccag
     1381 attttctcat cagtttgcga tcaaagttgt ggcgaatagg cttatcggaa cgacattcaa
     1441 atgatgtaat atcatttcct gttttaatat cctgaatctg aacacttgct tcaaaaggtg
     1501 atttagggtc tgcttctata tgacctacaa tataagattt tcctgcattt cttaattcga
     1561 gcgagattgt tgtgaattgt gaaccacctg caatctccga tgccggattt ttgagagctt
     1621 cttttacagg atttttgaat gtggtattac cgtgagagta cacatagttt cccccctctt
     1681 tggtaacttt taacgtttgg ttatctgtag ttgtgcaaga atacactaca ttgttatttg
     1741 caaaagcatt cattgagaaa aatgctgata aagctacaat tgccattttt ttcataggtt
     1801 agtttccttt tggtgcgttg ataatgtaat cggcttcctg gttacgtctt cgagcattaa
     1861 caccattatt atgcccttgt aaatttcgga ttgagttagc taaagccgtc atatcacctg
     1921 attgtgcgga gacggcagcc gctttttgaa cactttctag tttagataat gacccatagt
     1981 tgtatgatac cgacgttaat gctgcttgtg cttgaggagt caatttagcc caaacgtcgg
     2041 caccaacatt accccttgca acattcataa attgaggcat tcttcgtgct aaatcacgtt
     2101 cagcatcttc ccggcttaca taagtgcctt tttgaacccg atgaaccata ccatctgcag
     2161 tagtataggt gtcagagcca tatccggcac ggttagcatt tacatcccat ttcccgtggc
     2221 ttaaatatcc ttcaaactgt ttgataagag ccgttgcttg ttcattcgca ttgccgttca
     2281 tttgggcaat ttgggtatag ctccctacat tcacgttact gacaatgttt gttggggagg
     2341 ctttatatgc tccataaaaa tcactcatgc ctgagccgtc tgatttgggg atagcaatat
     2401 atctatcgcc aacggcattt tgtgcagaat tagcaatgcc actggtgttg agattatttg
     2461 ctccggcttg attgcttaaa ttggcaaagc gacggtttcc atcacccatc gcattatcat
     2521 caatagcaag agcttgttcg ttcatctgtt gagtaattgc agctaatgct gtattaccgt
     2581 tgattgaacg ttgcagttcc atttcgagtt taacttgctc aatttctctg tcaagtaatt
     2641 tgaggtttct ttctgcttct tctactaagg aagattttat aacatttggc tcacgcatac
     2701 cggctaacat tgctcggcga atcagtagca atttttccat ttctttagaa atagctaatt
     2761 cttgcgataa ccttgcaccc agcaaggctg catccggcga agcagcaatg gcttctaata
     2821 cgccttttgt tacactgacc gttgatgtac tcagtgaatt gagttgttcc ggcgttaaac
     2881 tatctaagtg attcagtgcc gcttctagct ttgtggcttt ttcaatagtt gtttgttcaa
     2941 taaaatgggc gattcctttc ccggctttag ctccacttga actttgttca caagtttcac
     3001 aggcatcaag gaatgtttca ccaatcactt cacttgcata ggcagcagcc tcgcttggat
     3061 ttttccattc cgtacaaagc ataccatcac agcttgcaat aggtatatct cctttttcta
     3121 atggtcctcg tcctaaagcg aggttatatc cggctccaac aatgtcagca acgatagcca
     3181 ttggtttttg atttttacca ccacgttgtt cgccacctac ccaagggatg cctttttcag
     3241 catctttttt atccattttc ttcttggctg ccattgcatc aggctctgtt tcagcaattt
     3301 cagcggctcg gtgtaatccg gccatctttg ctaatttact gtccattgct aaatcagcaa
     3361 gttggttaga catggcttcg caactggttt tgaagttgtt gaagtcaagt tttccttgat
     3421 atagaccatt agaaagaatg tcatacagtt gaggatttgc tcgttggata atcatcgccg
     3481 ggaggctggc aactgcaccg gttgctgact caataatatt gccataaaga tctttaaaac
     3541 cttcagtaat accattaagc tggtttttaa tggaaagttt aatatcaaag ttgccacaag
     3601 ttaaattcga tttccagcca atgccaatcc catattgatt tggtctgcca cgggaagctg
     3661 gtgcggaaat ggcactacct cctccaatgg agtagtgcat ataatcagaa aggacagggt
     3721 agtattccgt gagagcgtta ttaccagaaa cgcctagatt accagtccta tagcctgttg
     3781 tattttcaat aaaattacta atgctattgc caacattaac ggtattacta tttgcgtatc
     3841 cgctcatggt tgtgagcatt atacaagcgg ttagaaattt gagacttttt gcttttttca
     3901 tttatttatc ctaatagtgc tgataattta atgagtatgt gtacttttcc agtccacatg
     3961 acctaaaaaa acttgccctt gttttttaca gcaagaatag ggcctccata atgcccagct
     4021 gtaattctca gtattggaga aactatctga atgggtagcc caatcaccac tgtgcggaaa
     4081 gactttaggg taggattcgg aagtaaatac cttaactgtt acatctgagg gtttattagc
     4141 aggactagca cttgccatag ctgaaagcag tagtagtggt aaatagtaaa atgtcttagc
     4201 ttgcataaat gctccacaaa tagagtaatt cacgtttacc ttaaaagatg atttggttag
     4261 aagaaatcgg aaattgtttt ttggttttgg agagtttttg ttagtatata agttaatgta
     4321 acaagggaga gtggatgtat gagcaataga atacctaaag cctgttatgc ttacccaaaa
     4381 tcgccgaaca acccgtttaa tagattgtcg gttatcaatc tataccagcc gaaatgtctc
     4441 ccagcattct gaaagggaac tctgtaaaat tacaggaatg ccacctaaac aataaatgga
     4501 taatgccagc cactttatcc gttgtatttt gtcacatgcg tgattgcaat gtcataatat
     4561 tacccgactt ggggatatta tgacattgca atcattgctc acttttct
//
LOCUS       DANLYI010000178         4600 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.179, whole genome shotgun sequence.
ACCESSION   DANLYI010000178 DANLYI010000000
VERSION     DANLYI010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4600
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.179"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            139..468
                     /gene="trxA"
                     /locus_tag="PXD81_002093"
     CDS             139..468
                     /gene="trxA"
                     /locus_tag="PXD81_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018357021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HDL5538083.1"
                     /translation="MSNVIHTTDATFEQDVLKSDVPVLLDFWAPWCGPCRMIGPVLDD
                     LSVDAQFDGKVKIVKMNVDENPSVPAQFGVRSIPFLLLFKNGEVIAQQVGAIPHAQMA
                     EFIKQAL"
     gene            639..1862
                     /gene="hflK"
                     /locus_tag="PXD81_002094"
     CDS             639..1862
                     /gene="hflK"
                     /locus_tag="PXD81_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HDL5538084.1"
                     /translation="MSWNESGNQQDPWGKPGQKKAEPKPEQQEQGSQQEPTNNQRNEQ
                     QPPDLEEVFSSLLKKMGGGKGPNNSSNQPSASLGKFLPVVLGLAAVVWVGSGFYTVQE
                     AERGVVTRLGKLNDIVLPGLNWKPTFIDSVTRVNVERVSELNTSGSMLTQDENMVQVE
                     MTVQYRVEDPAKYLFSVSNPDDSLKQATDSALRYVIGHMTMDEILTTGRATVRERTWS
                     TLRDIIKTYDMGLLVTDVNFQYARPPEEVKAAFDDAIKAQEDEQRLIREAEAYARGEE
                     PIARGQAQRTIEQAQAYKEAVVLNAKGEVERLSQLLPEYKASPELTRERLYIQTMEKV
                     MKNTPKVVMDSSGNNLNVLPFDKLMNSSSVIKAEQPAAAQVVTPQVVQPQVQPRTQIQ
                     PQQAEQQEPTRKGRF"
     gene            1865..2752
                     /gene="hflC"
                     /locus_tag="PXD81_002095"
     CDS             1865..2752
                     /gene="hflC"
                     /locus_tag="PXD81_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HDL5538085.1"
                     /translation="MRRLLVPILAVVAFVVLQAITIVNEGERGIMLRFNKVHRDSDQK
                     VVVYEPGIHFKVPFIDSLKVLDARIQTLDGQEDRFVTVEKKDLLVDSYVKWRISDFGQ
                     FYTSTGGDYQKAADLLRRKVGDRLRSEIGSRTIKDIVSGSRGELMAGAQKALNAGEDG
                     AERLGIEVVDVRVKQINLPNEVSSSIYQRMRAERDAVAREHRSQGNEKAEVIRAEVDK
                     KVVLILANANKTAQALRGEGDAQAAKLYSEKFGNEPEFYSFIRSLKAYEDSFAEGQNN
                     MMLLKPNSEFLRFMQAPTK"
     gene            2926..4224
                     /gene="purA"
                     /locus_tag="PXD81_002096"
     CDS             2926..4224
                     /gene="purA"
                     /locus_tag="PXD81_002096"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010869263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HDL5538086.1"
                     /translation="MGKSVAILGAQWGDEGKGKIVDLLTDRVKYVVRYQGGHNAGHTL
                     IINGEKTVLRLIPSGILRDNVTCLIGNGVVLSPEALMKEMGELEERGINVRERLKISE
                     ACPLILPYHVAMDHAREAALGKNKIGTTGRGIGPAYEDKVARRGLRVSDLFDKEHFAA
                     KLKDILDYYNFQLVNYYKVEAVDYQKTLDDVFAIADVITGMVADVTTLLHEARENGDN
                     ILFEGAQGTMLDIDHGTYPFVTSSNTTAGGVATGSGYGPRHLDYVLGIIKAYCTRVGS
                     GPFTTELFDDVGAEIARKGNEFGAVTGRPRRCGWFDAVAVRRAVQINSISGFCMTKLD
                     VLDGFKELKICVAYKMPNGKIVEYAPLAAKDWEGVEPIYETMPGWSENTFRVTKREDL
                     PQAALDYIKRIEELVGVPVDILSTGPDRVETMILRDPFAA"
     gene            complement(4384..4454)
                     /locus_tag="PXD81_002097"
     tRNA            complement(4384..4454)
                     /locus_tag="PXD81_002097"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4424..4426),aa:Arg,seq:tct)
BASE COUNT         1424 a          812 c         1059 g         1305 t
ORIGIN      
        1 ctatgcaaat atttattggt gtaattggta aatcttgctt gaaattttat agattaatct
       61 ctatcttata caaaactgtt cgccttaact tgccagttag ggcgtttctt tttttattta
      121 atatttaagg ataaacttat gagcaatgtc attcatacaa cagatgctac atttgaacaa
      181 gatgttttaa aatcagatgt accggtatta ttagacttct gggcgccttg gtgtggtcct
      241 tgccgtatga tcggccccgt acttgacgat ttatctgttg atgcacaatt tgatggtaaa
      301 gtaaaaattg tcaaaatgaa cgtagatgaa aatccatctg taccagcaca atttggcgta
      361 cgcagcattc catttttact attattcaaa aatggtgaag tgattgccca gcaagttggt
      421 gcaattccgc acgcccaaat ggctgaattt atcaaacaag cattataatt ttaagagcaa
      481 tactagggcg aatttatcgc cctatttttt tgcaaataaa tagtgaaaaa agaccgcttg
      541 tttcagataa acctagtcaa aagcttacta aataggtaca atcactaact tgcattctaa
      601 gctttaaagt gcgattcgat tttattttgg agatatttat gtcgtggaat gaatcaggca
      661 atcagcaaga tccttggggt aaaccggggc agaaaaaagc tgagccaaag cctgagcaac
      721 aagaacaggg ttcacaacaa gagccgacca ataatcaaag aaatgaacaa cagccccctg
      781 atttagaaga agtatttagc agtttattaa aaaaaatggg gggtggaaaa gggcctaata
      841 atagttcaaa tcaacctagt gcaagcttag ggaaatttct acctgtggtt ttaggtcttg
      901 cagcagtagt ttgggttggc tcaggttttt acactgtgca agaggctgaa cgtggtgtgg
      961 ttactcgttt aggtaagctg aatgatatcg tgttgccagg gttgaattgg aaaccgactt
     1021 ttattgattc ggttacaaga gtaaacgtag aacgtgtttc agagctgaac accagtggct
     1081 caatgctgac tcaagatgaa aatatggtgc aggtagaaat gacggttcag tatcgtgtgg
     1141 aagacccggc taaatatctg tttagtgtga gcaaccctga tgacagctta aaacaagcaa
     1201 cagacagtgc cttgcgttat gtgattggtc atatgacgat ggatgaaatt ttaaccacag
     1261 ggcgtgcaac cgtgcgtgaa agaacgtggt cgacattgcg tgatattatc aaaacctatg
     1321 atatgggttt attggtaaca gacgtaaact tccaatatgc acgaccgcct gaagaggtga
     1381 aagccgcctt tgatgatgca atcaaagcac aagaagatga gcaacgctta attcgtgaag
     1441 cggaagccta tgcaagaggt gaagagccga ttgcacgtgg ccaggcacag cgtacgattg
     1501 agcaagctca agcttacaaa gaagcagttg tgttaaatgc gaaaggggaa gtggagcgtt
     1561 tgagtcaatt attacctgaa tataaagcct ctccagagct gactcgtgaa cgtttatata
     1621 tccagacaat ggaaaaagtc atgaaaaata cgccgaaagt ggtgatggat tcaagtggta
     1681 ataatttgaa tgtattgcct ttcgataagc taatgaattc ttcatcggta ataaaagcgg
     1741 agcagcctgc ggcggcacaa gtagttacac cacaagttgt tcagccgcaa gtacagccaa
     1801 gaacacaaat tcagccgcaa caagcagaac aacaagaacc aactcgtaag gggagatttt
     1861 aatcatgcgt agattattag tacctatact tgctgtagtt gcttttgttg tgctacaggc
     1921 aatcaccatt gtaaacgagg gcgagcgtgg cattatgtta cgttttaaca aagttcatcg
     1981 tgatagcgat caaaaagtgg tggtatatga accggggatt cactttaaag taccatttat
     2041 cgatagttta aaggtattag atgctcgtat tcaaactcta gatgggcaag aagaccgttt
     2101 tgtaacagtc gagaaaaaag atttattagt ggactcctat gtaaaatggc gaatcagtga
     2161 ttttggccaa ttttatacct ctactggggg ggattaccaa aaagcggcgg atttattacg
     2221 ccgtaaggta ggggatcgtc tgcgttccga gattggttct cgaaccatta aagatattgt
     2281 atccggctct cgtggtgagt taatggcagg agcccaaaaa gcgctgaatg ccggtgaaga
     2341 tggagcagag cgtttaggaa ttgaagtggt tgatgtgcga gtgaagcaaa tcaacttacc
     2401 aaatgaagtt tcttcttcaa tttaccaacg tatgcgtgcc gagcgtgatg cggttgctcg
     2461 tgaacaccgc tcacaaggta atgagaaagc agaggtaatt cgtgccgaag tggataaaaa
     2521 agtggtgctg attttggcaa atgcgaacaa aactgcccaa gccttgcgag gtgaaggtga
     2581 cgctcaagct gcaaaactct attcagagaa atttggtaac gagccggaat tttacagttt
     2641 tattcgtagt ttaaaagcct atgaagatag ctttgccgaa ggtcaaaata atatgatgtt
     2701 attaaagcca aacagcgaat tcttacgctt tatgcaagca ccgacaaaat aattgtatct
     2761 ataaaacccg atcttgtatc gggtttttaa ttttcatccc tattctgatc ataacaagcg
     2821 gtaaattttg gctgattttt tgcaaaaaat ttgaaaaaat tgaccgcttg cgatatgaag
     2881 ttataatggg tttcgttatt catcctaact aaaagaggca tttctatggg taaaagtgtt
     2941 gctattcttg gcgctcagtg gggcgatgag ggaaaaggca aaattgttga tttattaaca
     3001 gatcgtgtga aatatgtagt acgttatcaa ggtggacata atgcagggca taccttaatc
     3061 attaatggtg aaaaaaccgt tctgcgttta attccatcgg gtattttgcg tgataacgtg
     3121 acttgtttaa tcggtaatgg tgtagtgctt tctccagagg cattaatgaa agagatgggg
     3181 gagctagaag aacgaggcat taatgtgcgt gagcgtttaa aaatttctga agcttgcccg
     3241 ttaattctgc cttatcacgt tgcgatggat cacgctcgag aagcggcact tggtaaaaac
     3301 aaaattggta caacaggacg tggtattggc ccagcttatg aagataaagt cgctcgccgt
     3361 ggtttacgtg tcagcgattt attcgacaaa gaacattttg ccgcaaaatt aaaagatatt
     3421 ttagattact acaacttcca attagtcaat tattacaaag ttgaagcggt agattatcaa
     3481 aaaaccttag atgatgtgtt tgcgattgcg gatgtgatta ccggaatggt tgccgatgtg
     3541 acaactttac ttcacgaagc acgtgaaaac ggcgacaata ttttatttga aggggctcaa
     3601 ggcacgatgt tagatatcga ccacggtact tacccgttcg taaccagctc gaatacgaca
     3661 gcaggcggtg tggcaaccgg atcaggctac ggtcctcgtc atttagatta tgtattaggg
     3721 attatcaaag cctactgtac ccgtgtaggt agtggaccgt tcaccaccga actatttgac
     3781 gatgtaggtg ctgaaattgc tcgcaaaggt aatgagttcg gagcggtaac cggtcgccct
     3841 cgccgttgtg gctggtttga tgccgtagcc gttcgccgag cagtgcaaat caactccatt
     3901 tcaggcttct gtatgactaa actcgatgtt ttagacggat tcaaagagct aaaaatctgc
     3961 gttgcctaca aaatgccaaa cggaaaaatt gtcgaatatg cccctcttgc agcaaaagac
     4021 tgggaaggtg ttgaaccaat ttatgaaaca atgccgggtt ggtctgaaaa tactttccgt
     4081 gtgacaaaac gtgaagattt accacaagcc gcattagatt acattaaacg tatcgaagag
     4141 ctggtcggtg tgccggtaga tattctttct acaggtccgg atcgtgtcga aacgatgatt
     4201 ttgcgtgatc cttttgcagc ttaataaaag ataaaataaa gccatttagt gagatagagc
     4261 tagatggctt ttttgtttga accagtaaac tttactttgg cacaattttg tcacaataca
     4321 gaaaatagtt ctttagcgtg agtttttgag gctgtgtaag tatttgaatt taaaagaatt
     4381 taatggcacg ccctaaagga ttcgaacctt tgacccacgc cttagaaggg cgataggtat
     4441 tttataaaag gcgtttattt taactaataa aaacaaagac ttatagagat aacccctcta
     4501 tattacctac cattatttca catcttttta cattaaataa caccatttac ggtacattta
     4561 tggaacacgt tggtgatgag ctagtcttca aaagttacta
//
LOCUS       DANLYI010000179         4504 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.180, whole genome shotgun sequence.
ACCESSION   DANLYI010000179 DANLYI010000000
VERSION     DANLYI010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4504
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.180"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            101..532
                     /locus_tag="PXD81_002098"
     CDS             101..532
                     /locus_tag="PXD81_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDL5538087.1"
                     /translation="MSNQIALLLMSHGSFARAALASAELIVGQQENVAAIGVVAVDNV
                     ADIKRQMEQTIAELNTDKGLIILTDIVGGTPMNLASSQLTHPNVFACSGLNLPLLLEV
                     LMSRDGNLQEIRENLPLAYQNGFSLRTQQDFVSQEDDDDLL"
     gene            519..986
                     /locus_tag="PXD81_002099"
     CDS             519..986
                     /locus_tag="PXD81_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL5538088.1"
                     /translation="MICFSRIDDRLIHGQVVTTWVNLHRIEQIIVLNDAVAADPTQKQ
                     ILTMAAPQGIKVVAFGIEKFGEVLQKTQITRRTMLLFTNSIDVLRTVELGVSIDKLNV
                     GGMRYQARREQLSKAVSVTPEERQAFKTLLEKGVEVTVQMVPNDEKLNLQEII"
     gene            989..1756
                     /locus_tag="PXD81_002100"
     CDS             989..1756
                     /locus_tag="PXD81_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL5538089.1"
                     /translation="MLTALLVACWAGICALDDIGTQMIRRPLLIAPVVGLIMGDLETA
                     LIIGATLEVMWMGIGNVGAYSAPDMISGTAIGTALGIAAGEASTAVALAVPTSLLAQQ
                     LLILYRSGVCALNPIAERWAASGDFDKIFRIHYIPMLIAFSIRAIPTFLAVYYGADII
                     AQAVSALPAKVITGLNVAGKIIPAIGIGLLMLMMIKKAELWTFLIAGFTLAVYLKLSV
                     LPITLIALPLALIYDLAVSKKAEAAPTKTADEEDYDL"
     gene            1753..2559
                     /locus_tag="PXD81_002101"
     CDS             1753..2559
                     /locus_tag="PXD81_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDL5538090.1"
                     /translation="MSNKITKQDLNKVFWRMQVLNVTNNFQSMQAIGFLSSFIPVLKR
                     LYKDRPQEERAEAMKRHLKFFNSHVNSDALILGIAAAVEETTKEDEKETVTAIKTGLM
                     GPLAGLGDSLLKFTWLPICGSIGAALALSGNVLGPILMFLLYNSVNIATKYYGIHLGY
                     SKGVDLIHGAGNNILQRLGNMANVVGLMVIGALIASVVKVKVVSQIAAGDNVIKFQEM
                     LDKVMPGLLSLLLTVVIYWILKKTNGKYAATMILATMGICIGLVYAGILG"
     gene            2559..3596
                     /locus_tag="PXD81_002102"
     CDS             2559..3596
                     /locus_tag="PXD81_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HDL5538091.1"
                     /translation="MDSVYDIIRLEQGRYLFRQQPAQSVQIFFLDNPDSKDSKWLAET
                     DLAEFELKLSACEARPFFLIQSANGEQIVAERTLPVAGMNNFRDMGGYVAHQGKRVKW
                     GKLYRSDHLHNLRDEGVAYLDKLGIQTVIDYRSPNEVAKYPNPPINGREQTFRLDPNA
                     HTAELAAQFSADKHDEDRNLVNKIIAQKAEGNLINRYDIVMAQYRNFVEKAECQTAFA
                     EMLRLATDPENAPLVQHCRGGKDRTGFGAMLLLGILGVSKADIIADYMLTHYNRLARN
                     EEKMAIYRTFTQDQDVLDYLLSLIDTQPEFIEQSLNTIETQYGTIEQYAQRVLGITAK
                     EIEALRANYLA"
     gene            3629..4504
                     /locus_tag="PXD81_002103"
                     /pseudo
     CDS             3629..4504
                     /locus_tag="PXD81_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251836.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="histidine-type phosphatase"
BASE COUNT         1315 a          859 c         1041 g         1289 t
ORIGIN      
        1 tatttcttct tatgtgtacg atatatcgtg caataacttc ccttgtttat ctgtataaca
       61 ttttatttaa tagaatgact tataaatcaa ggagtgactt atgtctaatc aaattgcgtt
      121 attgttgatg agccacggca gttttgcgag ggcggcgttg gcgagtgccg agctgattgt
      181 cgggcagcag gaaaatgtgg cagctatcgg cgtagtagca gtggataatg tagcggacat
      241 taaacggcag atggagcaaa ccattgctga gttaaacacc gacaaaggct tgattattct
      301 caccgatatt gttggtggta cgccgatgaa tcttgcctct tctcaattaa cccaccctaa
      361 tgtttttgcc tgttcgggtt tgaatttacc tttattgcta gaagtgctga tgagccgtga
      421 cggcaatttg caagaaattc gggaaaatct accgcttgca taccaaaacg gatttagcct
      481 acgcacacaa caagattttg tttcacagga ggacgatgat gatctgcttt agccgtattg
      541 atgaccgttt aattcacggt caagtagtca ccacttgggt taatttacac cgtattgaac
      601 aaattattgt gttaaatgat gcggtggcag cggatccaac ccaaaaacag attttaacga
      661 tggcagcacc gcagggaatt aaagtggtcg cgtttggcat tgaaaaattt ggtgaagtgt
      721 tgcagaagac acaaattacc cgccgtacta tgttgttgtt taccaatagc attgatgtgt
      781 tacgcactgt agaactaggt gtatcgattg acaaattgaa tgtcggcgga atgcgttatc
      841 aagctaggcg tgagcagtta agtaaagcgg tttcagttac gccagaagag cgtcaagcat
      901 ttaaaacctt attggaaaag ggtgttgaag taacggtaca gatggtaccg aatgatgaaa
      961 aacttaactt acaggagatt atctagctat gctgactgca ctacttgttg cctgctgggc
     1021 aggtatttgt gccttagatg atattggcac acagatgatc cgccgtccgt tgttaatcgc
     1081 accggtggtc ggattgatta tgggggattt ggaaacggcg ttgattattg gtgctacgct
     1141 tgaagtgatg tggatgggga tcggcaatgt tggtgcatat tctgcaccgg atatgatttc
     1201 cggaaccgct attggcaccg cacttggtat tgctgctggt gaagcttcta ctgcggttgc
     1261 actcgcagta ccgacctcac tcttggcaca gcagttactc attctttacc gctccggcgt
     1321 atgtgcattg aacccaattg ctgaacgctg ggcggcaagc ggtgattttg acaagatttt
     1381 ccgcattcac tatatcccaa tgttaattgc cttttctatt cgtgctattc caacgttctt
     1441 agccgtctat tacggggcgg acattattgc ccaagcggtc tctgctctgc cggcaaaagt
     1501 gattaccgga ttaaatgttg caggcaaaat tattcctgcc atcggtatcg gtttgctgat
     1561 gttaatgatg attaagaaag ccgaactttg gacgttctta attgctggct ttacgttggc
     1621 ggtttatctc aaattatcag tattgccgat taccttaatt gcactaccgc ttgcgttgat
     1681 ttacgactta gcagtaagca aaaaagctga agctgcacca accaaaaccg cagatgaaga
     1741 ggattatgac ctatgagcaa taaaattacc aaacaagatt taaacaaagt gttttggcgg
     1801 atgcaggtgc tgaacgtcac aaacaacttc caatcgatgc aggcgattgg atttttaagt
     1861 agctttattc cggtattaaa acgactttat aaagatcgtc cacaggaaga acgagcggaa
     1921 gcaatgaaac gccacttaaa attttttaac agccacgtta attctgatgc attgatttta
     1981 ggaattgccg ctgcggtaga agagactacc aaagaggatg aaaaagagac agtaaccgcg
     2041 attaaaaccg gtttaatggg gccgttagca gggctgggag acagcttgtt gaaattcacc
     2101 tggttaccga tttgcggtag tatcggggcg gcattggcac taagtggcaa tgtgttaggc
     2161 ccgattttaa tgttcttgtt gtataacagt gtaaacattg cgacgaagta ttacggtatc
     2221 catctaggtt acagcaaagg ggttgattta attcacggtg cgggtaataa cattttgcaa
     2281 cgactcggca atatggcaaa tgtggttggt ttaatggtga ttggcgcatt aattgcctcc
     2341 gttgtgaaag tcaaagtagt ttcacaaatt gccgctggtg ataatgtgat taaattccaa
     2401 gagatgttag ataaagtgat gccgggacta ctcagcttac ttttaactgt cgtgatctac
     2461 tggattttga agaaaactaa cggcaaatat gctgcaacga tgatccttgc tacgatgggg
     2521 atctgtatcg gcttagtcta tgcaggaata ctcggctaat ggacagtgtg tatgacatta
     2581 ttcggttgga acagggcaga tacctgttcc gccaacagcc ggctcaatcg gtacagattt
     2641 tctttctgga caaccctgac agtaaggata gcaaatggct ggcggagact gatttggcag
     2701 agtttgagct gaaattgagc gcttgtgaag cccgtccgtt cttcctcatt caatcagcaa
     2761 acggagaaca gattgttgct gaacgcacct tacctgttgc cggaatgaat aattttcggg
     2821 atatgggggg ctatgtcgct catcagggca agcgagtgaa atggggcaag ttataccgct
     2881 ccgatcatct gcataattta agagacgaag gcgtggctta tcttgataaa ttgggaattc
     2941 agactgttat tgactatcgc agcccgaatg aagtcgccaa atacccaaat cccccaatta
     3001 atggcagaga gcaaactttt cggcttgatc cgaatgcaca tactgccgag cttgctgccc
     3061 aatttagtgc agacaagcac gatgaagatc gtaatttggt caataaaatc attgctcaaa
     3121 aagctgaagg taatcttatc aatcgctacg acattgtaat ggcacaatat cgtaattttg
     3181 tcgaaaaagc agaatgccaa accgcctttg ctgaaatgtt aaggttggcg acagaccctg
     3241 aaaatgcacc attggttcaa cactgccgtg gagggaaaga tcgcactggt ttcggtgcaa
     3301 tgttactgct tggtatttta ggcgtgagta aagctgatat tattgctgat tatatgctta
     3361 ctcattataa ccgccttgct cgtaacgaag agaaaatggc gatttaccgt accttcactc
     3421 aagatcaaga tgtgttggac tacctattgt ctttaatcga tactcaaccc gaatttatcg
     3481 aacaatcgct taatactatt gaaacccaat atggtactat tgagcaatat gcccaaaggg
     3541 tattaggtat tactgcgaag gaaattgaag cattacgggc aaattatttg gcgtagaacg
     3601 gtagggtgta atattgaaca cactacaaat ggagaaattt catatgcgtt attttgcaaa
     3661 acattggtat aaaactaccg cttgtctgac aacaattttc acagcatttc ctctttttgc
     3721 tcaaccaatt gaaaatatac caacttccca ctacgaatta gaaaaaatca ttatatttag
     3781 ccgacacggg ttacgttccc ctcttacaga aaaaggcagt gatttagaaa aatcaacccc
     3841 ttataaatgg gcaaattgga gtgtaccgac cggtcattta acccataaag ggacgctgtt
     3901 ggaaacctat tttgggcagt atcttggtca atggctcaca cataatgggt tattggacaa
     3961 ggcaagctgt gaaagtggga agcagattga ggtttatgct aatagcctgc aacgtacaat
     4021 tggcactgca caagctttgg ttgccggtgg gttttcaggt tgtaatgtac aagttaaaca
     4081 ccaccagcag attggaaaga tggatccggt gtttaatcca attattcgct ccgatgacca
     4141 gacatttgtg caaacagcat taagcaaaat tgacctcaat aaaacccatc agtatttagc
     4201 gccatcctat catttattaa gcgaaataat cgattatgct cattcagaaa aatgtttgca
     4261 ggaaaaacag tgtgaatttt ttaatgatgt tggtaaatta aggattaaaa aggataaata
     4321 accaggtgtt tccggtacga tgcgattaag taaaaatatt gtcgatgcct tattgctgca
     4381 atattatgaa ggttttcctt taaaagagat tgcaaataac cagcttgata gtgatcagaa
     4441 atggcaaaca ctcaataaaa ttaaaaacga ttaccaattt atgctacgag gagatccatt
     4501 tatt
//
LOCUS       DANLYI010000180         4482 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.181, whole genome shotgun sequence.
ACCESSION   DANLYI010000180 DANLYI010000000
VERSION     DANLYI010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4482
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.181"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            201..1520
                     /locus_tag="PXD81_002104"
     CDS             201..1520
                     /locus_tag="PXD81_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HDL5538092.1"
                     /translation="MNHLLYSVEDKPPFGLSLLLASQHLLAALGGIIAVPLVLGNVLK
                     LSTADTIILVNAALLISGIVTIIQCRGIGPIGIRLPSVMGTSFTFVAAALAIGFSESG
                     IAGIMGASLVGSLVMIIGSFFMPYIRKLFPPVVTGTVIMMIGLSLIPVAVDWFAGGQR
                     TDPHYATPENLIMASFVLIIVVALVQWGKGIFSAAAIVIGMMIGYIVSLALGWVSFNG
                     VKEAQLFALPQPLHFGLEFPISGIIGMSIAYLVTIVESSGNFLALGNATKTEITGKHL
                     RGGVLCDGLGSAFAAIMSTTPFSSFSQNIGVISLTGVASRYVVAFTGALLVLAGLFPV
                     FGALIVSIPLPVLGGAGLMMFAMIIAAGIQMLDNIQRSKRNGLIIAISIGCGLAVTTR
                     PELLDKLPHFFKEVFGSGITVGSLLALILNLVLPEDKITKKENKSDE"
     gene            complement(1690..3633)
                     /gene="ftsH"
                     /locus_tag="PXD81_002105"
     CDS             complement(1690..3633)
                     /gene="ftsH"
                     /locus_tag="PXD81_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HDL5538093.1"
                     /translation="MVKNILLWVVIAIVMMTAYEGFNSNFSGSSNTVPYSTFLSDIKE
                     NRLKGVDFKQNDDTILVTKNDGATYQTVMPMYDEYLMADLAKTNAVITGQPAERRGLL
                     SQILISWFPMLLLIGFYVFYMRQMQGGGGRGAMGFGKNKAKMLTADEVKTRFSDVAGC
                     DEAKEEVGEVVEFLRDPTKFQKLGGRIPKGILMVGPPGTGKTLLAKAIAGEARVPFFT
                     MAGSDFVEMFVGVGASRVRDLFDQAKKNAPCIIFIDEIDAVGRKRGGAGFSGGHDERE
                     QTLNQMLVEMDGFEGSEGVIIIAATNRADVLDDALTRPGRFDRQVTVDLPNVKGREQI
                     LKVHLKKVPLAEGVDPMQIARGTPGYSGAQLANLVNEAALFAARKNKRVVMMEDFEEA
                     RDKINMGPERRSNTMTEKEIINTAYHEAGHVIVGYLMPEHDPLNKVTIVPRGQALGFA
                     QFLPEGDRVSETFTKLESQLSTLFAGRIAEGLIFGEDKITTGASSDIHRATQIARAMV
                     TQWGFSKELGPIFYQNEDGMGAIKGVSEESQKLIDVEMRKIIDRNYQRAKQTLEENMD
                     ILHAMKDALLKYETLDSKQIDDLMARRPVGEPSGWTDTPTKDDNATPPSGEQAADVSS
                     TLEDNPNKDNNIGDSLADTKAEH"
     gene            complement(3754..4380)
                     /gene="rlmE"
                     /locus_tag="PXD81_002106"
     CDS             complement(3754..4380)
                     /gene="rlmE"
                     /locus_tag="PXD81_002106"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011200140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HDL5538094.1"
                     /translation="MGRKRSASSSRWLAEHFKDQFVQKAHKQKLRSRAYFKLDEIQQT
                     DRLFKPGMTVVDLGAAPGGWSQYVVTQIGSKGRVIACDILDMNPIVGVDFLQGDFREE
                     SVLNALLERVGEDKVNVVMSDMAPNFSGMPSVDIPRAMYLVELALDMCRQVLATKGSF
                     VVKVFQGEGFDEYLREIRSLFSVVKVRKPEASRDRSREVYIVATGYKG"
BASE COUNT         1223 a         1018 c          829 g         1412 t
ORIGIN      
        1 ttttttgtta caactggaaa gaaattggta caaacattcg gattagcttt gcaaaaagcg
       61 taaaaaatct taccgcttgt catttatcag ttagggaaaa taaaaaatca ctctcttata
      121 taaaaacaat tacgcaaacg attgcttatt aaaaaaactt cagtataatg ccgacatttt
      181 ttaactctcg aggaatcact atgaaccatt tactttattc tgtcgaagac aaaccgcctt
      241 tcgggctgag cttattgctt gcctcacaac atttattagc cgctttaggt ggcattatcg
      301 ctgtaccttt agtgctaggg aatgtactca aactctctac tgcggatact ataatcttag
      361 ttaatgctgc acttttaatt tcaggcatcg tcactattat tcaatgtcga ggtattggcc
      421 caatcgggat tagactaccg agtgtaatgg gaactagctt tacgtttgtt gcagcagcat
      481 tagcgattgg tttcagtgaa agtggcattg ccggcattat gggagcctct ttagttggct
      541 ctctagtgat gattatcggc agttttttta tgccttatat ccgtaagctt tttccacccg
      601 tggtaacagg tactgtcata atgatgattg gcctaagttt aattcctgtt gcagtagact
      661 ggtttgctgg tggtcagcgt actgatccac actatgcaac cccagaaaat cttataatgg
      721 ctagttttgt attaattatt gtcgttgctt tagtacaatg ggggaaaggt attttttcag
      781 ctgctgctat tgtgattggt atgatgatag gttatatcgt ttcgctagct ctgggttggg
      841 taagttttaa tggcgtgaaa gaagcacaac tttttgcgtt accacaacct ctacattttg
      901 gcttagaatt tccaatctcc ggtatcatcg gaatgtcaat tgcttattta gttaccattg
      961 tagaatctag cggaaatttc ttagcactag gcaatgcaac taaaactgaa attaccggaa
     1021 aacacttacg tggaggtgta ttatgtgatg gtttaggctc agcatttgcg gcaattatgt
     1081 ccacaactcc cttttcttct ttctcacaaa atattggggt catttcatta accggtgtag
     1141 ccagtcgcta tgttgtagca tttacaggcg cattacttgt actagccggt ttatttcctg
     1201 tatttggagc attaatcgtg tctatcccgt tacctgtgct tggcggtgca ggcttaatga
     1261 tgtttgccat gattatcgca gccggtatcc aaatgttaga taatattcaa cgtagcaaac
     1321 gaaacggttt aattattgct atctctatcg gttgtgggct ggccgtaaca actcgacctg
     1381 agctattgga taagcttcca cacttcttta aagaagtttt tggctcaggt attacggtcg
     1441 gctccttatt agcacttatt ttgaatttag ttttacctga agataaaata accaagaaag
     1501 aaaataagag tgatgaataa atcaccctaa aaaaacaagg acagctcaca atacaacttt
     1561 gatgctgtcc tttattccca tagagagcat atttaaattt aggttaataa aaaaatctgc
     1621 actctatatt ggtgtttacc tttagagtac agatttcttc ttattctaac tttctagcaa
     1681 acaattagat taatgttccg ctttggtatc ggctaacgaa tccccaatgt tattatcttt
     1741 atttggatta tcctctaaag ttgaacttac atccgctgcc tgttcaccac ttggtggagt
     1801 ggcgttatcg tctttagttg gggtatctgt ccaacctgat ggttcaccca ccggacggcg
     1861 tgccattaaa tcatcaattt gcttgctgtc taaggtttcg tatttcaata aggcatcttt
     1921 cattgcgtgt aagatgtcca tattttcttc caaagtttgc ttcgctcgtt gatagttgcg
     1981 gtcaatgatc ttacgcattt ccacatcaat caatttttgc gactcttccg atacgccctt
     2041 aattgcaccc attccatctt cattttgata gaaaatcggg cctaattctt ttgagaagcc
     2101 ccattgagta accattgctc gtgcaatttg ggttgctcga tgaatatcag aggaagcccc
     2161 tgtggtaatt ttatcttcac caaaaatcaa gccctcagca attcgccctg caaatagggt
     2221 ggaaagttgg ctttccaatt tggtaaatgt ttcgcttaca cggtcacctt ccggtaagaa
     2281 ttgagcaaaa cctaacgcct gaccacgagg cacaattgtc actttattca gcggatcgtg
     2341 ttccggcatt aaatagccaa caataacgtg tccagcttcg tggtaagcag tattgatgat
     2401 ctctttttcc gtcattgtat ttgaacggcg ttccggcccc atattgattt tgtcacgggc
     2461 ttcttcgaaa tcttccatca ttaccacacg tttattctta cgggcagcaa ataaggcagc
     2521 ttcgttcacc aagtttgcta attgagcacc agagtaaccc ggcgtaccac gagcaatttg
     2581 catcgggtca actccctctg caagcggtac ttttttcaag tgaactttca aaatttgctc
     2641 acgccctttc acgttcggca aatcgaccgt gacttggcgg tcgaaacgcc ccggacgagt
     2701 taaggcatca tctaacacat cggcacggtt ggttgcagcg atgataatca caccttcaga
     2761 gccttcaaag ccgtccatct caactaacat ttggttgagg gtttgttcac gctcatcgtg
     2821 tccaccgctg aaacctgcac caccacgttt acgccctacc gcatcaattt catcaataaa
     2881 aataatacaa ggggcatttt tcttggcttg gtcgaataaa tcacgcaccc gtgaagcacc
     2941 cacacctaca aacatttcca cgaagtcgga acctgccatt gtaaagaatg gcactcgtgc
     3001 ttcacctgca atcgctttcg ccaataaggt tttacccgta cccggagggc ctaccatcaa
     3061 gatccctttt ggaatgcgcc cacctaattt ttgaaattta gttgggtcac gcaaaaactc
     3121 aacaacttcg cctacttctt ctttggcttc atcacagccg gctacatcag aaaatctggt
     3181 cttaacttcg tctgcggtca acattttggc tttatttttc ccaaagccca ttgcaccacg
     3241 tcctccgcca ccttgcattt gtcgcatata aaacacatag aaaccgatta acagcaacat
     3301 tgggaaccaa gaaatcaaaa tttgcgagag caagccacga cgctcagcag gctgaccggt
     3361 aatcactgca ttggtttttg ctaaatctgc cattaagtac tcatcataca tcggcattac
     3421 ggtttggtag gttgcaccat catttttcgt caccaaaatg gtgtcatcat tctgtttgaa
     3481 atcgacccct tttaaacgat tttctttaat atcgcttaaa aaagtcgaat atggcactgt
     3541 atttgagcta ccactgaaat tagaattaaa accttcatag gctgtcatca tcacaatggc
     3601 aatcaccacc caaagtaaaa tatttttaac catatcgttc aaggttaatc ccctatctgc
     3661 tgaataaaaa actgttcaag attactataa aagcggtcga attttccaat ttttttgcaa
     3721 aatgttaata aaaaatgacc gcttgcttgt tttctagcct ttataacctg tcgctacaat
     3781 ataaacttct cgagaccgat ctcgagacgc ctccggtttt ctgactttta ccacactaaa
     3841 gagcgagcgg atttctcgaa ggtattcatc aaatccttcg ccttggaata ccttaaccac
     3901 aaaactgcct ttggttgcta atacttggcg acacatatcc aatgccaatt ccactaaata
     3961 cattgcacga ggaatatcaa ccgacggcat accgctgaaa ttgggagcca tatccgacat
     4021 caccacattc actttgtctt cgcctactcg ctcaagtaac gcattcagca ccgattcttc
     4081 acggaagtcg ccttgtaaga aatccacgcc tacaatcggg ttcatatcta aaatatcaca
     4141 agcgattact ctgcccttac tgccgatttg cgtcaccaca tattgcgacc aaccgcccgg
     4201 tgcggcaccg agatccacca ccgtcattcc cggtttgaat aaacggtcgg tttgttgaat
     4261 ttcatctaac ttaaaatacg cccgtgaacg caatttttgt ttatgtgctt tttgcacaaa
     4321 ttgatcttta aaatgttctg caagccaacg tgatgaactc gcacttcgtt ttctacccat
     4381 ttgttcttct atcttaatta attcaacgat ttataataag ggttaaatta gcaaattcaa
     4441 gagatgttct aaaatttagc tcggcttatt atgcttaatt aa
//
LOCUS       DANLYI010000181         4470 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.182, whole genome shotgun sequence.
ACCESSION   DANLYI010000181 DANLYI010000000
VERSION     DANLYI010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4470
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.182"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            131..4027
                     /gene="purL"
                     /locus_tag="PXD81_002107"
                     /gene_synonym="purI"
     CDS             131..4027
                     /gene="purL"
                     /locus_tag="PXD81_002107"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HDL5538095.1"
                     /translation="MTIQTFRGSPALSAFRIQQLLQKFQQNQLPVKSVYAEFLHFVAL
                     NRPLVADEENKIKELLHYGPTLEEDEPTGFCLIVTPRIGTISSWSSKATDIAHNCGLA
                     AVERIERGTAYYLEFSETPNESQLTTLKGLLHDRMMETVLDNESQAEQLFAQQEPKPF
                     TSVDILGGGRKALEEANINLGLALADDEIDYLVENFTQLGRNPNDIELYMFAQANSEH
                     CRHKIFNADWTIDGVKQEKSLFKMIKNTFEKTPDYVLSAYKDNAAVMEGSTVGRFFPD
                     QDGQYRYHQEDAHILMKVETHNHPTAISPFPGAATGSGGEIRDEGATGRGAKPKAGLT
                     GFSVSNLVIPKFEQPWENPLSKPNRIASALDIMIDGPLGGAAFNNEFGRPALLGYFRT
                     YEEKVNSFAGEEIRGYHKPIMLAGGIGNIRAEHVQKGEIPVGAKLIVLGGPAMNIGLG
                     GGAASSMASGKSKEDLDFASVQRENPEMERRCQEVIDRCWQMGAENPILFIHDVGAGG
                     LSNAMPELVHDGECGGKFDLRNILCDEKGMSPLEIWCNESQERYVLAVSPEKLDIFTA
                     LCERERAPFAVIGEATKEKHLTLADPHFDNKPIDLPMNVLLGKTPKMQRDVLSKTVKN
                     PPLATADIDLKEAFHRVLRLPAVAEKTFLITIGDRSVTGMVAQDQMVGPWQIPVADCA
                     VTTASLDSYHGEAMSIGERSPVALLDFAASARLAVAESITNIAATNIGDIKRIKLSAN
                     WMSAAGHEGEDAGLYEAVKAVGEELCPELGITIPVGKDSMSMKTTWEENGEQKSVTAP
                     LSLVISAFARVEDVRKTVTPQLRTDKGDTRLLLIDLGEGNNRLGATALAQVYKQLGDK
                     PADVVNVARLKGFFNAMQTLVAQGKLLAYHDRSDGGLITTLAEMAFAGNCGISVEIDR
                     LVDDLAVLFNEELGAVIQVRTEELSEVQAVLNAENLANLTFDIGTAVESDSFEITRNG
                     TTVFSQKRSELRGIWAELTHQMQRLRDNPECADQEFAAKKDPNNKGLSAFLTYDVNED
                     IAAPYINKGVKPSVAILREQGVNSHYEMAAVFDRAGFNAIDVHMSDLISGRRNLNDFN
                     AMVACGGFSYGDVLGAGGGWAKSILFNPMLRDQFSKFFANPNTLALGVCNGCQMVSNL
                     AEIIPGTENWPRFVRNKSERFEARAAMVKINDTNSLWFNGMAGSHMPIAVSHGEGQVE
                     FKNPQQLANLQQQNLVIAQYVDSHLNPTEQYPANPNGSALGITAIGNTDGRVAIMMPH
                     PERVFRAVSNSWCPEDWTEGGAWMRLFRNARVALKY"
     gene            4382..4470
                     /locus_tag="PXD81_002108"
                     /pseudo
     CDS             4382..4470
                     /locus_tag="PXD81_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248253.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
BASE COUNT         1247 a         1022 c         1109 g         1092 t
ORIGIN      
        1 tattgaacaa atttatttga tactaatgaa taaatgtatc taacagcaaa cgtctgacaa
       61 atatagcaat cgtttgcgca atcgtttata atcggtttag tttttcatct taaataggaa
      121 aatccaaaga atgacaatcc aaaccttccg tggctcgcct gccttatctg cttttcgcat
      181 tcaacaactt ctgcaaaaat tccaacaaaa tcaattacct gtaaaatcag tttatgcgga
      241 gtttttgcat tttgtggcat taaatcgacc gcttgtagcc gatgaagaaa acaaaatcaa
      301 agagttattg cactacggtc caacgctgga agaagacgag ccgacagggt tttgcctgat
      361 tgttacccct cgtatcggca cgatttcctc gtggtcgtct aaggcaaccg acatcgccca
      421 taactgcggt ttggcagcag tagagcgaat tgagcgtggc acggcatatt atttagaatt
      481 ttctgaaacg ccgaacgaaa gtcaacttac gacgttgaaa ggcttattac acgaccgtat
      541 gatggaaacg gtgctggata acgaaagcca agccgaacag cttttcgctc agcaagagcc
      601 gaagccgttt acttcggtgg atattttagg cggtggtcgc aaggcactgg aagaagcgaa
      661 tatcaactta ggtttagcgt tggcggacga tgaaattgat tacttggtgg aaaactttac
      721 ccaacttggg cgtaacccca atgatattga actctatatg ttcgcccaag ccaactcgga
      781 gcattgccgc cacaaaattt ttaatgcgga ttggacgatt gatggggtaa aacaggaaaa
      841 atccctgttt aagatgatta aaaacacctt tgagaaaacc ccggactatg tgctttccgc
      901 ctacaaagac aatgcggcgg tgatggaggg ctcaacggtt gggcgtttct tccccgacca
      961 agatggacaa tatcgctatc atcaagaaga tgctcatatt ttaatgaaag tagaaactca
     1021 caaccaccca accgccatct cgccattccc gggagcggcg accggttcgg gcggtgaaat
     1081 tcgtgatgaa ggggcgacag ggcgtggggc gaaaccgaaa gcgggcttga cgggtttttc
     1141 ggtctctaac ttagtgatcc caaaatttga acagccgtgg gaaaacccgc tttccaagcc
     1201 gaatcgcatt gcctccgcac tggatattat gattgatggc ccactaggcg gagcggcatt
     1261 taacaatgaa tttggtcgtc ctgcgttatt gggctacttc cgtacttacg aagaaaaagt
     1321 gaacagcttc gcaggcgaag aaattcgtgg ctatcacaag ccgattatgt tagcaggcgg
     1381 tatcggcaac attcgtgccg aacacgttca aaaaggcgaa atcccggtgg gagcgaagtt
     1441 gatcgtgctt ggtggcccgg caatgaacat cggcttaggc ggtggggcgg catcgtcaat
     1501 ggcatcgggg aaatcgaaag aagatttgga ttttgcctcg gttcagcggg aaaaccctga
     1561 aatggaacgc cgttgccaag aggtgatcga ccgttgctgg cagatggggg cggagaaccc
     1621 aattttattt attcacgacg tgggggctgg cggtttatca aatgcgatgc ctgaattggt
     1681 tcacgacggc gaatgtggcg gtaagtttga tttgcgtaac atcttgtgcg atgaaaaagg
     1741 aatgtcgcca cttgaaattt ggtgtaacga atcgcaagag cgttatgtgc ttgccgtttc
     1801 ccctgaaaaa ctcgacattt tcacagcatt gtgcgaaagg gaaagagccc catttgcggt
     1861 gattggcgag gcaacgaaag agaagcattt aacccttgct gaccctcatt ttgacaataa
     1921 accgattgat ttgccgatga atgtgttgct cggcaaaacg ccaaaaatgc agcgagatgt
     1981 tttgtcaaaa actgtgaaaa atccaccgct tgcaaccgct gacatcgact tgaaagaggc
     2041 gttccaccgt gtgctacgct tgcctgcggt ggctgaaaaa accttcctga ttaccatcgg
     2101 cgatcgctca gtaacgggaa tggtggcaca agatcagatg gtcggcccgt ggcaaattcc
     2161 tgtggcagat tgtgccgtaa ccaccgcttc attagacagc tatcacggtg aagcaatgtc
     2221 aattggcgag cgatcacctg ttgctttgct tgactttgct gcctcagctc gcctcgccgt
     2281 ggcagaaagc atcaccaata ttgccgccac taacattggc gacatcaaac gcattaaact
     2341 ctcggcgaat tggatgtcgg cggcagggca cgagggcgaa gacgcagggc tttacgaggc
     2401 agtaaaagcg gtgggcgaag agctttgccc tgaattaggc ataaccattc cggtaggcaa
     2461 agactcaatg tcgatgaaaa ccacttggga ggaaaacggc gaacagaaat ctgttactgc
     2521 tccgctttcg ctcgtgattt ccgctttcgc tcgtgtggaa gatgtgcgta aaaccgttac
     2581 cccacaactt cgcaccgaca aaggcgatac ccgcttgttg ctgatcgacc tgggcgaagg
     2641 caacaaccgc ttgggtgcaa ccgcacttgc tcaagtttac aaacaacttg gcgacaaacc
     2701 tgccgatgtg gtgaatgtgg ctcgcttaaa aggcttcttc aacgcaatgc aaacccttgt
     2761 cgcacaagga aaattgcttg cctaccacga ccgctcggac ggtggtttaa tcaccacact
     2821 ggcggaaatg gcgtttgcag ggaattgcgg aatttcggtg gaaatcgacc gcttggtgga
     2881 cgatttagcc gtgttattca acgaagaact gggggcagta atccaagttc gcaccgaaga
     2941 attgagcgag gtgcaagcgg tgttaaatgc cgaaaatctt gcaaatctca cctttgatat
     3001 tggtacagcg gttgaaagcg acagctttga aattacccgc aacggcacaa cagtatttag
     3061 ccaaaaacgt tccgaactgc gtggcatttg ggcggaactc actcatcaaa tgcagcgttt
     3121 gcgagacaac cctgaatgtg cagaccaaga atttgcagcg aaaaaagacc cgaataacaa
     3181 aggcttatcc gcatttttaa cttacgatgt gaacgaagac atcgccgccc catacatcaa
     3241 caaaggcgta aaaccgagtg tggcgatttt gcgtgagcaa ggtgtaaaca gccactacga
     3301 aatggcagcc gtctttgacc gtgccggttt caacgccatt gacgtgcata tgtcagactt
     3361 gatttcaggc agacgcaacc tcaacgactt caacgcaatg gtggcgtgcg gaggcttctc
     3421 ttacggcgat gtcttaggtg caggcggcgg ctgggcgaaa tcgattctgt tcaacccaat
     3481 gctgcgtgac caattcagca aattcttcgc caacccgaac acgctggcat taggggtatg
     3541 taatggttgc caaatggtct ccaatctagc ggaaattatt ccaggcacag aaaactggcc
     3601 acgcttcgtg cgtaataaat ccgaacgttt tgaagcccgt gctgcaatgg tgaaaatcaa
     3661 cgacaccaac tcactttggt tcaacggaat ggcaggcagc catatgccga ttgcggtttc
     3721 acacggtgaa ggacaagttg agttcaaaaa tccacagcaa ttagcgaact tgcaacagca
     3781 aaatctcgtg atcgcacaat atgtggatag ccacctcaac ccaaccgaac aatacccagc
     3841 caacccgaac ggatcggcgt tgggtatcac cgctatcggt aatacagacg gacgagtcgc
     3901 tattatgatg ccacaccctg aacgtgtgtt ccgtgcggtc agtaactctt ggtgcccaga
     3961 agattggacg gaaggtggag cgtggatgcg gttgtttaga aatgcgagag tggcgttgaa
     4021 gtattaaaaa tctaccttcg tagtgatctg cccccatagg gggcgattaa aattcgaatt
     4081 catagaaccc gaatttttat atatctgata aacgaagttg tttatccttc gtgcttttcc
     4141 tgatatttca agtactttct tatcatttct gcattgacat ctacggtgtc tacgcagtag
     4201 cctcttgcca caaaagtggg ttgccccaca atttatgctt acgtaaatag ggaaacttat
     4261 tgaaaaatct tattggaccc tgttcaccct tttaaatgcc ccattacgtc ttatacagaa
     4321 aatgaacgtg atttatttga acggtcaatt ctaccttaat agaaagtggt ttttagttag
     4381 caatcaaaat cacattaacg gaattgagaa tttggagagt caggcaaaat gctatttgcg
     4441 taaatttaac ggcgttccta aagtacatga
//
LOCUS       DANLYI010000182         4388 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.183, whole genome shotgun sequence.
ACCESSION   DANLYI010000182 DANLYI010000000
VERSION     DANLYI010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4388
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.183"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            <1..853
                     /locus_tag="PXD81_002109"
     CDS             <1..853
                     /locus_tag="PXD81_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional regulator GcvA"
                     /protein_id="HDL5538096.1"
                     /translation="ESAARHLNFTKAAEDLFVTQAAVSHQIKLLEDFLGASLFHRRNR
                     LLELTELGAQYFYEVQPLLEQIALATDKIRQQSSRQILTISVPQTFGMHWLVPRLADF
                     HEKYPEVEVRIKGVDQDEGLLGKETDVAIYYGKGDWQGLESIRLTESRIVILASPEYL
                     SQNTIHLPADLAGKHLLHSSTHNKWKRMVEHLHIEDIVDAETGSIFSHTFMVLQAAMH
                     QQGIAIANQLLAQHEIEKGTLIEPFPTTLFDEKSFYVVYPSQSAQVQKVQFFVEWIQQ
                     KMKECCA"
     gene            929..1612
                     /gene="yjjG"
                     /locus_tag="PXD81_002110"
     CDS             929..1612
                     /gene="yjjG"
                     /locus_tag="PXD81_002110"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HDL5538097.1"
                     /translation="MKYQWVLFDADETLFSFNSYLGLKAMLARYQIDFSEQDYQDFQA
                     VNKPLWVAYQNNEITAQDIQTRRFVKLSAQTGVDPLQLNQELMAEMAFVSQPLEGVMD
                     MLNALYGKVKMGIITNGFTDLQQKRLDNTQTSHFFDIVVISEQIGAAKPDRQVFDYAF
                     ALMDEFDRTKVLMVGDTLASDILGGNNAGIDTCWLNLLSKENDTDIKPTYEVHTMQQI
                     IAIVEGKSL"
     gene            1609..2592
                     /gene="cysB"
                     /locus_tag="PXD81_002111"
     CDS             1609..2592
                     /gene="cysB"
                     /locus_tag="PXD81_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HDL5538098.1"
                     /translation="MKLQQLRYIVEIVNQNLNITEAAEVLYTSQPGISKQVRLLESEL
                     GINIFERNGKHVKGLTPAGEKIVAIARELLVKAQSIKSVAEEQTRPNKGILRIATTNT
                     QARYMLPSVIEKFKARYPEVSLHLHQGSPNQIYDALVAGEVDLAITTEAQYLFDEAVL
                     LPCYWWNRSVIVKPEHPLAAFVNQSKNLTVEQLGRYDLITYTFGFTGQSDLDHAFNKE
                     GILPNIVFTATDADVIKTYVRLGLGVGIMASMAHTETDKDLVAINASHLFQPSMTQIA
                     FKRGTFLRNYMYDFINYFSPHLTRVQVEKAEQLRDNNAISRLFESISLEEK"
     gene            2593..3237
                     /locus_tag="PXD81_002112"
     CDS             2593..3237
                     /locus_tag="PXD81_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5538099.1"
                     /translation="MRIWQKLASVIVVTLGLISNASAENFKLKYSSNYLMPAYINFNN
                     GGGQYNIQAKINVPLYNIVFSAKGTEKNEQFHMLSYRDTRNGKTYAMAEIDSKTVKYG
                     KVKEPLKEEALTMPTYDLFTTAFQLSYYDKLPSSFQTTNGKKLYPTPNVQLRKAQKEV
                     KVGGKTYQEITYKFRTSDKKDITVKKFEGEKFPRYIAYSRDGDNYELTFDSFVK"
     gene            complement(3311..4360)
                     /gene="ansB"
                     /locus_tag="PXD81_002113"
     CDS             complement(3311..4360)
                     /gene="ansB"
                     /locus_tag="PXD81_002113"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HDL5538100.1"
                     /translation="MKIKKLLLPTLLSIATMPIQAADLPNITILATGGTIAGSQKNTA
                     TSAYQAGQLNIETLIEAVPDIKKLANIKGEQIVKIGSQDMSDEVWLKLAKAINQQCPN
                     TDGFVITHGTDTMEETVYFLDMTVKCEKPVVLVGAMRPATEKSADGVLNLYNSVVVAK
                     DSKSSGRGVLVAMNDIVLGARDVTKTSTTSVQTFNSPNFGALGYIHNSKVDYERSPES
                     KHTVNTPFKVDELTELPKVGIIYAYSNMPTEPLQALLDASYQGIVVAGVGNGNMNAAN
                     LALLEEAAKKGITVVRSSRVPTGYTTRDAEIDDSKYGFIASGTLNPQKARVLLQLALT
                     QTNDIKTIQQYFEDF"
BASE COUNT         1343 a          777 c          910 g         1358 t
ORIGIN      
        1 tgaatctgct gcacgccatt taaattttac taaagcggca gaagatctct tcgtcacaca
       61 agctgcagtg agccaccaaa ttaaactatt agaagatttt ttaggtgctt ccctgttcca
      121 tcgccgtaac cgtttgctgg aattaactga gttaggcgca caatatttct atgaagttca
      181 gccattatta gaacaaattg ctctcgcaac agataaaatt aggcagcaaa gtagtcgtca
      241 aattctgact attagcgtac cacaaacatt tggtatgcat tggttagtcc cacgtttggc
      301 ggatttccac gaaaaatatc cagaagtgga agtacgtatt aagggtgttg atcaagatga
      361 gggattatta ggtaaagaaa cagatgttgc tatttattat ggtaaggggg attggcaagg
      421 tctggaatct atccgtttga cggaatcccg tattgttatt ttagcttcgc ctgagtattt
      481 atcgcaaaat accattcatt taccggcgga tttagcaggt aagcatttat tacattcctc
      541 cacccataat aagtggaaaa gaatggttga acatttgcat atagaagata tagtagatgc
      601 ggaaactggc tcaattttta gccatacttt tatggtgctt caagctgcaa tgcaccaaca
      661 aggcattgcg attgccaacc agttgttggc acagcacgaa attgaaaaag gtactttaat
      721 agagcctttc ccaaccacat tgtttgatga aaaatcgttc tatgttgtct atccttcgca
      781 atcagcccaa gttcagaaag tacaattttt tgttgagtgg attcagcaaa aaatgaaaga
      841 gtgttgtgct taagcgtggc tttatcagcg gtatgcgaaa aattagaaaa atttgaccgc
      901 ttgttatcta agttaaaaaa ggaatattat gaaatatcaa tgggtattat ttgatgctga
      961 tgaaaccttg ttttcattta attcttatct tggtttaaaa gcgatgctgg ctcgttacca
     1021 aattgatttt agtgaacaag attatcaaga ctttcaagcg gtaaataaac cactttgggt
     1081 ggcttaccaa aataatgaga tcactgctca agatattcaa accagacgtt ttgtaaaatt
     1141 atctgctcaa acaggcgtag atccgctaca acttaatcaa gagttaatgg cggagatggc
     1201 attcgtcagt cagcctttag aaggtgtaat ggatatgtta aatgctttat atggtaaagt
     1261 aaaaatgggc attatcacca atggttttac cgatttgcag caaaaacgtt tagataatac
     1321 tcaaacttca catttttttg atattgtagt gatatcagaa caaattggtg ccgcaaagcc
     1381 tgatcgccaa gtgtttgatt acgcttttgc attaatggac gaatttgatc gtactaaagt
     1441 gcttatggta ggcgataccc tcgcttctga tattttagga ggtaataatg ctggaattga
     1501 tacttgttgg ttaaatttgc ttagtaaaga gaatgatacg gatattaagc caacttacga
     1561 agtgcatacg atgcaacaga ttattgcgat tgtagagggg aaatcattat gaagctacaa
     1621 cagttacgtt atatcgttga aatagtaaac cagaacttga atattaccga ggccgcagag
     1681 gtgctttaca cttctcaacc ggggattagc aaacaagttc gtttgttgga aagtgagttg
     1741 ggaattaata tttttgagcg taacggtaag cacgttaagg ggctaacgcc tgctggtgaa
     1801 aaaattgtgg cgattgctcg tgaattattg gtaaaagccc agagcattaa atcggttgct
     1861 gaagaacaaa ctcgacccaa caaaggcatc ttacgcattg cgaccaccaa tacccaagcc
     1921 cgttatatgc taccgagtgt aattgagaag tttaaggctc gctatcctga agtaagtttg
     1981 catttgcacc aaggctcccc gaatcaaatt tatgatgctt tggtggctgg agaagtggat
     2041 ttagccatta ccacagaagc tcaatattta tttgatgaag cggttttatt gccgtgttat
     2101 tggtggaatc gttcagttat tgtcaaacca gagcatcctc ttgcggcttt tgtgaaccaa
     2161 agtaaaaatt tgactgtcga acaattaggt agatatgatt tgataaccta tacttttggt
     2221 tttacagggc aatcggattt agatcatgcc ttcaataagg aaggtatttt gcctaatatc
     2281 gtatttacgg cgacagatgc tgatgtaatc aaaacttatg tacgcttagg cttaggggtt
     2341 ggtattatgg catcaatggc acatacggaa acggataaag acttagtcgc tatcaatgca
     2401 agtcacctgt ttcaaccgag tatgacgcaa atcgccttta aacgaggaac ttttcttcgt
     2461 aactatatgt atgattttat taattatttt tcgccacatt taacacgggt acaagtggag
     2521 aaagcggaac aattacgaga taacaatgca atttcaaggt tgttcgaaag tatatcttta
     2581 gaggagaaat agatgagaat ttggcagaag ttagcgagcg taatcgtggt cactttgggc
     2641 ttgatttcaa acgccagtgc ggagaatttt aagttgaaat acagctcaaa ttatttaatg
     2701 ccagcctaca ttaactttaa taacggtggt ggccaatata atattcaggc aaaaattaat
     2761 gtgcctttat ataatattgt cttttctgcg aagggaacag aaaaaaacga acaatttcat
     2821 atgttgagct accgagatac ccgtaacggc aaaacttatg cgatggcaga aattgattct
     2881 aaaacggtaa aatatggcaa agtgaaagag ccactaaaag aagaagcgct gactatgcca
     2941 acttatgatt tgttcaccac agcctttcag ttgagctatt acgataaatt accgagcagt
     3001 tttcaaacta ctaacggtaa aaaactttac ccaacaccga atgtgcagct acgcaaagcc
     3061 caaaaagagg taaaagtagg cggcaaaacc taccaagaaa ttacctacaa gtttagaacc
     3121 agtgataaaa aagacattac ggtgaaaaaa tttgaaggtg aaaaattccc tcgctatatc
     3181 gcctatagtc gtgatggcga caattatgag ctaacttttg atagctttgt aaaataataa
     3241 agattagtaa tgagacaagc ggtctatttt gtgatttgtt ttgcaaaata gaccgcttgt
     3301 tatttacttt ttagaaatct tcaaaatatt gttgaatagt ttttatatca tttgtttgtg
     3361 ttaaggcgag ctgcagtaat actcgagctt tttgaggatt taatgtgccg gaagcaataa
     3421 aaccatactt gctatcatca atttctgcat ctcgggtagt atatccggtt ggcactcgag
     3481 acgagcgaac cacggtaatt ccttttttag ctgcttcttc aagtaaagcc aaattagccg
     3541 cattcatatt gccattgcca actcctgcaa ccacgatgcc ttgataactc gcatctaata
     3601 atgcttgaag cggttcggtt ggcatatttg aataagcata aataatgccg acttttggca
     3661 attccgttaa ttcatctact ttgaaagggg tgtttacggt gtgtttactt tcaggagagc
     3721 gctcataatc aactttgcta ttatggatat agcctagtgc gccaaagttt ggtgagttaa
     3781 atgtttgcac tgatgtagta ctggtttttg taacatcgcg agcgcccaat acaatatcat
     3841 tcattgcaac caacacccct ctgccactag atttagaatc tttggcgaca acaactgagt
     3901 tatataaatt taatacgccg tcggcacttt tttcagttgc tggtcgcatg gcccccacta
     3961 atactacggg tttttcgcat tttacagtca tatcaaggaa gtatacggtt tcctccatag
     4021 tgtctgtgcc gtgcgtaata acgaagccat ctgtgtttgg gcattgttga ttaattgctt
     4081 ttgccagttt tagccatact tcatcagaca tatcctgaga tccgatttta acgatttgtt
     4141 ctcctttgat atttgctaat tttttaatat ccgggacggc ttctatgagt gtttcaatat
     4201 ttagctgccc agcttgataa gctgaggttg ccgtattttt ttggctaccg gcaattgtcc
     4261 ctccagttgc aagaattgta atattaggta ggtcggctgc ttgaattggc atagtagcaa
     4321 tgcttaacag agttggcagt agtaattttt tgattttcat tgtatcgtct ccagtattag
     4381 aaaaacag
//
LOCUS       DANLYI010000183         4385 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.184, whole genome shotgun sequence.
ACCESSION   DANLYI010000183 DANLYI010000000
VERSION     DANLYI010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4385
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.184"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            complement(<1..1129)
                     /gene="dnaJ"
                     /locus_tag="PXD81_002114"
     CDS             complement(<1..1129)
                     /gene="dnaJ"
                     /locus_tag="PXD81_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015939959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HDL5538101.1"
                     /translation="MAKKDYYEVLGLSKGASEKDIKRAYKRLAAKHHPDKNQGSKESE
                     EKFKEITEAYDVLTDSEKKAMYDQYGHAAFEQGGAGAGGFGGFGGGGFGGFEDIFSEM
                     FGGGFGGGGRRQRVVRGDDLRYDIEITLEEAVKGCKKDIRIQTLAECDTCHGTGAEAG
                     SKVETCSHCHGSGRVRRQQGFFVTEAVCPSCHGTGKRIEKPCRSCHGDGRVQKAKNLS
                     VTIPAGVDTGNQLRLSREGAAGENGAPAGDLYVVIHVKEHDIFERDGSNLYCEVPISF
                     TLAALGGEIEVPTLDGKLKLKIPAETQTGKLFRVRGKGVTSPRGGYAGDLICKVVVET
                     PVNLTEEQKALLRQFEESLEGQGKHRPKHEGFFDGVKKFFDN"
     gene            complement(1352..1654)
                     /locus_tag="PXD81_002115"
     CDS             complement(1352..1654)
                     /locus_tag="PXD81_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5538102.1"
                     /translation="MLKLYPTTENVQPPFYKIYDHKSGETVIATETENSNTAILMDDF
                     VWFEQIPTTEALLADTWSVIENRSKQGLLKRAYTKDFEKHLADGKVEIYIAVNPHC"
     gene            complement(1796..3694)
                     /gene="dnaK"
                     /locus_tag="PXD81_002116"
     CDS             complement(1796..3694)
                     /gene="dnaK"
                     /locus_tag="PXD81_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005670448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HDL5538103.1"
                     /translation="MGKIIGIDLGTTNSCVAVMDGDKPRVIENAEGARTTPSIIAYTD
                     KETLVGQPAKRQAITNPKNTLFAIKRLIGRRFTDAEVQRDIEIMPFEISKADNGDAWV
                     TVKGDKLAPPQISAEILKKMKKTAEDFLGEPVTEAVITVPAYFNDAQRQATKDAGRIA
                     GLDVKRIINEPTAAALAYGLDSKKENQTIAVYDLGGGTFDISIIEIDNFDGEQTFEVR
                     ATNGDTHLGGEDFDNRVINYLVEEFQKEQGVDLRNDPMAMQRVKEAAEKAKIELSSAQ
                     ETEVNLPYITADATGPKHLNIKVTRAKLESLVEDLVNRSLEPLKTALADAGLSVGDIN
                     DVILVGGQTRMPLVQKKVADFFGKEPRKDVNPDEAVAIGAAVQGGVLSGSVTDVLLLD
                     VTPLSLGIETMGGVMTTLIEKNTTIPTKKSQVFSTAEDNQSAVTIHVLQGERKRAADN
                     KSLGQFNLEGINPAPRGMPQIEVTFDIDANGIINVSAKDKNTGKEQQIKIQASSGLSD
                     AEVEQMVRDAEANAEADKKFEELVQARNQADGIAHATRKQITEAGDALNADDKAKIEA
                     AISELETAAKGEDKAEIEAKIEALIKASEPLMQAAQAKAQGGEQPQQSQKDDGVVDAE
                     FEEVKDNK"
     gene            3953..4291
                     /locus_tag="PXD81_002117"
     CDS             3953..4291
                     /locus_tag="PXD81_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDL5538104.1"
                     /translation="MKLITTIIQPSKLDQVQDALSEIGIQGMTITEVKGFGRQYGHTE
                     QELSQMSRIDFRPKIKVEILVSNYQTDEVIDAILNASYSGQVGDGKIYVANIEQVIRT
                     RTGETDDEAV"
BASE COUNT         1107 a         1020 c          878 g         1380 t
ORIGIN      
        1 agttatcaaa gaatttcttc acaccatcaa agaaaccttc gtgtttcgga cgatgtttac
       61 cttgtccttc taaactctct tcaaattgtc gtaatagtgc tttctgttct tcggttaaat
      121 tcacaggcgt ttcgaccacc actttgcaga ttaaatcccc tgcgtagcca ccacgagggc
      181 ttgttacccc tttgcctcgt acacggaata gtttaccggt ttgagtttct gccggaattt
      241 tgagttttaa tttgccgtct aaagttggca cttcaatttc accgcctaaa gctgcaaggg
      301 taaagctaat cggcacttca cagtataaat tcgaaccgtc acgctcaaaa atatcgtgtt
      361 ctttaacgtg aatcaccaca tataaatcgc ctgccggtgc gccattttcg cctgcagctc
      421 cctctctcga taagcgaagt tgattaccgg tatccacacc tgccggaatg gtaacagaga
      481 ggtttttcgc tttctgtaca cgtccatcgc cgtggcaaga acggcacggt ttttcaatgc
      541 gtttacctgt accgtgacaa cttggacaaa ctgcctcagt cacaaagaaa ccttgctgac
      601 ggcgaactct gccggagccg tgacaatgcg aacaggtttc cactttgctc cctgcttccg
      661 cccctgtacc atgacaagta tcacattcag ctaaggtttg aatgcgaata tcttttttac
      721 agcctttcac agcttcttca agggtgatct cgatgtcgta acgtaaatca tcgccacgta
      781 ccacacgttg gcgacgtcca ccgccaccaa agccaccgcc aaacatttcg ctgaagatgt
      841 cttcaaaacc accgaaacca ccgccgccaa agccaccgaa tccgcctgca ccggcaccgc
      901 cttgttcaaa ggcggcgtga ccgtattggt cgtacatcgc ttttttctcg ctgtcggtca
      961 gcacgtcata ggcttcggta atctctttaa atttctcttc cgactcttta ctgccttggt
     1021 ttttatccgg gtgatgcttt gccgccaaac gtttatatgc tcgtttgatg tctttctcgc
     1081 ttgccccttt agacagtcca aggacttcgt aataatcttt ctttgccatt gttgttccgt
     1141 ttgtaaattt ttctgtgttt ttgaccgctt gccctcgccc cttgtgggag aggggtagga
     1201 tggttaattc cccctctctc tgaaaaatat ttctgaacga aatatttttc ggtctctctc
     1261 ccacaagggg agagagtttt atataattaa atgggatcta gccaagatgg ctcaagatga
     1321 cacaagcgtg gacgcttgcg ctatcaaaag cctaacaatg cggattcacc gcaatataaa
     1381 tttctacttt gccgtctgct aaatgttttt caaaatcttt ggtgtaagct cgtttgagca
     1441 aaccttgttt ggaacggttt tcaatcacag accaagtgtc tgccaaaaga gcttcggtag
     1501 ttggaatttg ctcaaaccaa acaaaatcgt ccattaaaat cgcggtattg ctgttttccg
     1561 tttctgtggc aatgaccgtt tcgccacttt tatgatcgta tattttataa aacggtggct
     1621 gtacattttc cgttgtaggg tagagtttta gcatttgtaa aatttccttt gaaaagcacc
     1681 gcttgcacag ccccaaaggg gctgaattat gtgtaaccta gcacggctcg tgctaggtgg
     1741 tatatatcta cacaaaaggg gcgaaagata tttcgcccct acaagttacg caagattatt
     1801 tattatcttt cacttcttca aattccgcat cgaccacgcc gtcatctttt tgagattgtt
     1861 gtggttgctc gccgccttgt gcttttgctt gtgctgcttg cattaacggt tctgaagctt
     1921 tgattaacgc ttcgattttc gcttcgatct cggctttatc ttcgccttta gctgcggttt
     1981 ctaactcaga aatagccgct tcgatcttcg ctttatcgtc tgcgtttaac gcatcgcctg
     2041 cttcggtaat ttgcttacgg gttgcgtgag caataccgtc tgcttggtta cgagcttgaa
     2101 ctaactcttc aaattttttg tctgcttccg cattcgcttc tgcatcacgc accatttgtt
     2161 caacttcagc atcgcttaag cctgaagatg cttgaatttt gatttgttgc tctttgccag
     2221 tgtttttatc ttttgcagag acattgataa taccgttagc atcaatatca aaggttactt
     2281 caatttgtgg cataccgcgt ggagcagggt taataccttc aaggttgaat tgacctaaag
     2341 atttattatc cgctgcacgt ttacgctcac cttgtaacac gtggatagtt accgcagatt
     2401 ggttatcttc cgctgttgag aacacttgtg atttcttggt tggaatcgta gtgtttttct
     2461 caattaaggt agtcattaca ccacccattg tttcgatacc aagtgaaagc ggagtaacgt
     2521 ctaataacaa tacgtctgtt accgaaccac ttaacacacc gccttgaacc gccgcaccga
     2581 ttgccacagc ttcgtccggg ttaacgtctt tacgcggttc tttgccaaag aagtctgcca
     2641 cttttttctg tactaacggc atacgggttt gaccaccgac taaaatcaca tcgttgatgt
     2701 cgcctactga taaaccggca tcagctaaag cggtttttaa cggctctaac gaacggttta
     2761 ctaaatcttc aactaaagat tccaatttcg cacgggttac tttaatgttt aagtgtttcg
     2821 ggccggttgc atctgccgtg atgtatggaa ggttcacttc agtttcttgt gcagaagaaa
     2881 gttcgatttt cgctttttcc gctgcttctt tcacacgttg cattgccatt gggtcgttgc
     2941 gtaaatcaac gccttgttct ttttggaatt cttccactaa gtagttgatt acacggttat
     3001 cgaagtcttc accacctagg tgagtatcgc cgttggttgc acgcacttca aaagtttgct
     3061 cgccgtcaaa gttatcaatt tcgataatcg aaatatcgaa tgtaccacca cctaagtcgt
     3121 aaacagcaat cgtttggttc tctttcttag aatctaaacc ataagcaagc gctgccgctg
     3181 ttggttcgtt gatgatacgt tttacatcta aacctgcaat acgaccggca tctttagttg
     3241 cctgacgttg tgcatcgtta aagtatgcag gcacagtaat aaccgcttcg gttaccggtt
     3301 cgcccaagaa atcttctgcg gttttcttca tttttttcaa gatttcagca gagatttgcg
     3361 gcggtgctaa tttatcgcct tttacggtta cccacgcatc gccgttatct gctttgctga
     3421 tttcaaacgg cataatttca atatcacgtt gcacttcagc atcagtaaaa cgacggccga
     3481 ttaaacgctt gattgcaaat aaagtatttt tcgggttagt aattgcctga cgttttgccg
     3541 gctgaccaac taaggtttct ttatcggtgt aagcgatgat agatggcgtt gttcttgccc
     3601 cttccgcatt ttcaattaca cgaggtttgt cgccgtccat taccgcaaca caagaatttg
     3661 ttgtacctaa gtcaatacca atgattttac ccatttttat gattcctata ataataaatt
     3721 cagtttgaat aagaaacggg attgcatttc ccgttcagac gaaatcataa atagggacga
     3781 aaatgcactg ttcaagggca aaaataaaaa aattgcaaaa tttttggaaa aagtaaccgc
     3841 ttgtatgtgg gggagatttg gcagaatatt tcacggagaa aatacctcct accttgcata
     3901 cccctcaata gctttttata cttacggcaa ctcaaacaga gaaggatata atatgaaact
     3961 gattactacc attatccaac cttcaaaatt agaccaagtg caagatgcac tttcagaaat
     4021 cggtattcaa ggaatgacta ttaccgaagt aaaaggcttt ggacgccaat atgggcatac
     4081 cgaacaagag cttagccaaa tgagccgcat tgactttcgt cctaaaatta aggtggagat
     4141 attggtttct aattaccaaa ccgatgaagt gatcgatgcc attctcaatg catcctattc
     4201 tgggcaagtg ggtgatggca aaatttatgt agcgaatatt gaacaagtta tccgcacccg
     4261 taccggcgaa actgacgatg aagcagtcta gccattgcta gcagagaagc tttttattat
     4321 cgtgttttag agttatattt tcaaccttac taaagcaaat tttctaattt ggtgtgaatt
     4381 aaatg
//
LOCUS       DANLYI010000184         4384 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.185, whole genome shotgun sequence.
ACCESSION   DANLYI010000184 DANLYI010000000
VERSION     DANLYI010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4384
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.185"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            2..595
                     /locus_tag="PXD81_002118"
     CDS             2..595
                     /locus_tag="PXD81_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007524923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HDL5538105.1"
                     /translation="MALSLTALVVSSMSVAGTLSTSSMVDILAFDGQRVKSGTTSVQM
                     SENKTHQVVVEVGGTVEGDFFTSDQIILTFQGSAENAKLETPRLNSKLDLRKFQENQT
                     VAIKTDSGKVISHKQDFLKGEGFLAKTRVEDNLTKYNLAKKTASVEQFATAPLEAKGQ
                     IVVDTNKVSQEELQLLFRKADKDTQKRFLEWAKKNIK"
     gene            complement(676..1059)
                     /locus_tag="PXD81_002119"
     CDS             complement(676..1059)
                     /locus_tag="PXD81_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5538106.1"
                     /translation="MKTLKLGCIFSLALMLSSCTLTPEQQAERKAKQIRAEQDLQVQL
                     AKQCDVETAELMYQQFNPPLSQTEQEEKAFKKRYAEKVNAPMFQACYKLAWQNYKAQV
                     ELEEIRWNYDRDYMYRGWRYCYYCW"
     gene            complement(1089..1658)
                     /locus_tag="PXD81_002120"
     CDS             complement(1089..1658)
                     /locus_tag="PXD81_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HDL5538107.1"
                     /translation="MNETTINDFSLLCRLFGNLFYRHPTDPILAGTFNWLAQGGLRQV
                     WALNTDSQSENALTLLQKNANPTQLAASYQALFAENGAISPTISAYGLSVNNFVAFRQ
                     ERAMPELENADHIGLLFLTASWIEDNLDSALAQKILFEQFLLPCAGKFLGKIEAFDNG
                     FYKALAQLSRDALSAMADELEESELEAVE"
     gene            1717..2562
                     /gene="nfo"
                     /locus_tag="PXD81_002121"
     CDS             1717..2562
                     /gene="nfo"
                     /locus_tag="PXD81_002121"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HDL5538108.1"
                     /translation="MKYIGAHVSASGGVENAVLRSVEIGANAFALFTKNQRQWQAPAL
                     KAETIEKFKRFCKVHHFSAEQILPHDSYLINLGNPEAEALEKSRVAFIDEMSRANQLG
                     LKLLNFHPGAHLKQISEQDCLARIAESINIAVEKVPNVIAVIENTAGQGSNLGWHFDH
                     LATIIEQVEDKSRVGVCLDTCHLFSAGYDISSYEKSEAVFNDFNQTVGFHYLRGMHLN
                     GSKTPLGSRVDRHHTLREGTIGTEVFAFIMQNMHFNHIPMILETISPEIWADEIKFLR
                     TLELK"
     gene            2690..3262
                     /locus_tag="PXD81_002122"
     CDS             2690..3262
                     /locus_tag="PXD81_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HDL5538109.1"
                     /translation="MKKNLFATTFKPVFIASILSVFGGYSSIAVASLSSKPATPNQII
                     ASKAERLAKSSSVFTNRANLPTSSAHTANYSGRINSVYHNWVGTRYRLGGTTKAGIDC
                     SAFVREVMGKAFSKDMPRSTAEQRHVGHSISKSDLRPGDLVFFRKNNHVGVYIGNGKF
                     VHASSSRGVTTSSLSESYWSRNYTQSRRVI"
     gene            complement(3329..3787)
                     /locus_tag="PXD81_002123"
     CDS             complement(3329..3787)
                     /locus_tag="PXD81_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDL5538110.1"
                     /translation="MASIRQISDQTAAYQPIPLYRDFNSSRSVKLDLNSLCIKRPRDT
                     FFIHVKNPNLIAWGIELDDLLIVEQTNQYLVNDLLVLEKDGEYKFYQFFNEIQNGEGI
                     AEKILFSLDVSESNLRITDWAEVKIAGVITNVVHQMRTRGAFTQAKQRVA"
     gene            complement(3961..4344)
                     /gene="grcA"
                     /locus_tag="PXD81_002124"
     CDS             complement(3961..4344)
                     /gene="grcA"
                     /locus_tag="PXD81_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HDL5538111.1"
                     /translation="MIKGVQITESSNSNLLNSFWLLDEAKNEARCLAAKAGFEEDQVV
                     AISELGDIAYREVPVNVAPTIKVEGGQHLNVNVLRRETLEDAIQNPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVITRTFTESL"
BASE COUNT         1360 a          766 c          875 g         1383 t
ORIGIN      
        1 aatagcatta agtttaacag ccttagttgt cagttcgatg agtgtggccg ggacgttaag
       61 tacatcatca atggtggaca ttttagcctt tgacgggcaa cgtgttaaat ccggcacgac
      121 ttcggttcaa atgagtgaga ataaaacgca tcaagtggta gtggaagtcg gtgggacagt
      181 tgaaggggat tttttcacct ctgatcaaat cattttgact ttccaaggca gtgcggaaaa
      241 tgcaaaatta gaaacgccaa gattaaattc aaagttagat cttcgtaaat tccaagagaa
      301 tcagactgtg gcaattaaaa ccgattcagg taaggttatt tcacataaac aagatttctt
      361 aaaaggggaa ggtttccttg cgaaaacaag agttgaagat aatttaacga aatataactt
      421 agcgaaaaaa acagcttcag ttgaacagtt tgcgacggca cctcttgaag caaaaggaca
      481 aattgtggtg gatacaaata aagtttcaca agaagaatta cagcttttat ttagaaaggc
      541 agataaagac acccaaaaac gtttcttaga atgggcgaag aaaaatataa aataaaccta
      601 tatcaattaa taagcggtca tttctcgcca attttttgca aggtgttaaa aaaatgaccg
      661 ctcatacaat gagatttacc agcaataata gcagtaacgc cagcctctat acatatagtc
      721 tctatcgtaa ttccaacgaa tttcttcaag ttctacttgt gccttgtaat tttgccaagc
      781 aagcttataa caggcttgga acattggggc attgactttt tcggcatagc gtttcttaaa
      841 tgctttctct tcttgttcgg tttggctaag cggcggatta aattgttggt acattaactc
      901 cgccgtttct acatcacatt gtttggcgag ctgaacttgc aaatcttgtt ctgcccgaat
      961 ttgcttcgct ttacgttcag cttgttgctc tggagttaaa gtacagctac ttaacataag
     1021 tgcgagtgaa aaaatgcagc ctaatttgag tgttttcatt gctgaatcct ctagttgaaa
     1081 ttggtgattt attctaccgc ttctaactca ctttcttcaa gttcatcagc catagcacta
     1141 agggcatcac ggctaagttg tgcgagggct ttataaaaac cattgtcgaa agcttctatt
     1201 tttcctaaga atttgcctgc acaaggtagt aaaaattgtt cgaataatat tttttgagct
     1261 aaggcagaat cgagattatc ttcgatccaa gatgccgtga gaaatagtaa cccaatgtga
     1321 tcggcatttt ctaattcagg cattgcccgc tcttgtctaa aagctacaaa attattaact
     1381 gataatccat aggctgaaat tgttggagaa atagccccat tttcagcaaa aagcgcttga
     1441 tagcttgctg ctaattgcgt agggttagca ttcttttgta agagagtcag cgcattttca
     1501 ctttggctat ccgtatttaa tgcccagact tggcgtaatc ctccttgggc aagccagtta
     1561 aatgtgccag ctaaaatagg gtcggtaggg tgacgataaa ataagttgcc aaacaaacgg
     1621 caaagcaggg aaaaatcgtt aatggtagtt tcgttcataa tttgaaatag atagaaaaaa
     1681 gaattgttag tatagcagaa cacaagagag gactgaatga aatatatagg cgcacacgtt
     1741 agtgcttcag gtggggtgga aaatgcagtt ttgcgttccg tagaaattgg tgctaatgca
     1801 tttgcgttat ttactaagaa tcaacgtcaa tggcaggcac cggcattaaa agccgaaact
     1861 atcgaaaaat ttaagcgttt ttgtaaagtg catcattttt cagcagagca aatcttacca
     1921 cacgatagct atttgattaa tttaggaaat ccggaagcgg aggcattgga gaaatctcgt
     1981 gtagcattta ttgatgagat gtctcgagca aatcaattag gtttaaaatt gttgaatttt
     2041 cacccggggg cgcatttaaa gcaaatttcc gagcaagatt gtttggctag aattgcagaa
     2101 tcgatcaata ttgcagttga aaaagtaccg aatgttattg ccgtgattga aaatacggca
     2161 ggacagggtt ctaatttagg ttggcatttc gatcatttgg caactattat tgagcaagtt
     2221 gaggataaaa gccgagtggg ggtgtgttta gatacttgtc acttattttc tgccggctat
     2281 gatattagtt cttatgaaaa gagcgaggca gtatttaatg attttaacca aacggttggt
     2341 tttcattatt taaggggaat gcacttaaat ggttcaaaaa caccattagg cagcagagtg
     2401 gatcgtcatc ataccttaag agaaggcaca ataggaaccg aagtgtttgc atttattatg
     2461 caaaatatgc attttaacca tattcctatg attttagaga caatttcgcc tgaaatttgg
     2521 gcagatgaaa taaaatttct acgcacattg gaactaaaat aggttatact ggtctgagta
     2581 gtgggtctag cagagtctag accttatttt gttatctttt ttaatctttt agaagattta
     2641 gttaaggaat ttaccgctgt aattgttctg gcgagacgag acataaatta tgaaaaaaaa
     2701 tctttttgca acaaccttca agcccgtatt tattgcttct attttgtctg tttttggagg
     2761 gtattcttcc attgctgtag caagtttaag ttctaaaccg gccacaccaa atcaaatcat
     2821 tgcaagtaaa gcagaaagat tagctaagtc ttcttcagta tttaccaatc gagcaaatct
     2881 tccaacttct tccgctcata cagcgaatta tagtggtcgt attaatagtg tttatcataa
     2941 ttgggtaggg acacgctatc gtttaggcgg cacaacaaaa gcaggaattg attgttccgc
     3001 ctttgtgcgt gaagtaatgg gcaaagcatt tagtaaagat atgcctcgtt caactgcaga
     3061 acaacgccat gtcgggcatt ccatttcaaa atctgattta cgcccaggtg atttagtctt
     3121 tttccgtaag aataaccatg taggggttta tattggtaat ggtaaatttg tacacgctag
     3181 ttcaagtaga ggggtaacca ccagctcatt gtcagaaagc tactggtcaa gaaactacac
     3241 acaatctcgc cgtgtaattt aataaattac aattccaaga tgaaaagaac actcactttt
     3301 gcgagtgttc ttttttaatt aaaaaagttt atgctacgcg ttgcttagct tgtgtaaatg
     3361 ctccacgagt acgcatttga tgaaccacat tagtaattac acctgctatt tttacttcag
     3421 cccaatcagt aattctaaga tttgattcgc tcacatctaa tgaaaataaa attttttcag
     3481 caataccttc cccattctga atttcattaa agaattgata gaatttatat tccccatctt
     3541 tttcaagcac caatagatca ttaactaaat attggttggt ctgttctaca atcaataaat
     3601 cgtctagttc aattccccac gcgattaaat ttgggttttt cacgtggata aaaaaagtgt
     3661 ctcttgggcg tttgatacaa agtgagttaa gatcgagctt tacagagcgg ctgctattga
     3721 aatcccgata aagcggaatc ggctgataag ctgccgtttg atctgaaatt tgtctaattg
     3781 aagccatagt aaaatcctct atctgttttt ttatacaggg tattttactg gctaaaatac
     3841 cagtgtcaac cattattttg gaattttttg taaatttaac cagtgttttg accgcttgtt
     3901 atcctttaaa aagcaataaa aaagcaccca tagggtgctt cttatgctta aaagctgtta
     3961 ttataggctt tcagtgaatg tacgagtaat aacatcgcgt tgttgctcag gggttaatga
     4021 gttgaaacga actgcataac ctgaaacacg gatcgttaat tgaggatatt tctcagggtt
     4081 ttgaatcgca tcttctaatg tttcacggcg taaaacgttt acgtttaagt gctgaccacc
     4141 ttcaacttta atcgttggtg caacattaac gggtacttca cggtatgcga tatcacctaa
     4201 ttcgctgata gcaacaactt gatcttcttc aaatcctgct tttgcagcta aacaacgagc
     4261 ttcattttta gcttcgtcta ataaccagaa agagtttaat aagttgctgt ttgatgattc
     4321 tgtgatttgt acacctttaa tcattttgat atctcctcta agattgaaat tataaaaaaa
     4381 tttt
//
LOCUS       DANLYI010000185         4324 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.186, whole genome shotgun sequence.
ACCESSION   DANLYI010000185 DANLYI010000000
VERSION     DANLYI010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4324
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.186"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            46..1152
                     /gene="recA"
                     /locus_tag="PXD81_002125"
     CDS             46..1152
                     /gene="recA"
                     /locus_tag="PXD81_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HDL5538112.1"
                     /translation="MAEKKSQKNTPVKQIDPEQKEKALAAALAQIEKQFGKGSIMKLG
                     DTQALDIEAVSTGSLGLDSALGIGGLPMGRIVEIYGPESSGKTTLTLSVVAQAQKNGK
                     TCAFIDAEHALDPIYARKLGVDTDGLLISQPDNGEQALEICDALVRSGAVDVIIVDSV
                     AALTPKAEIEGDMGDSHMGLQARLMSQALRKLTANIKATNCLVIFINQIRMKIGVMFG
                     NPETTTGGNALKFYASVRLDIRRSGVVKDGDEVIGSETKVKIVKNKVAPPFREVQFDI
                     MYGEGIARMNELLILAESHGFINKAGAWFSYEGEKIGQGKNNAIKWLKEHPEVASKIE
                     QDIRNLLISNPTFTATPDSENADNADDEFSEEEL"
     gene            1211..1687
                     /gene="recX"
                     /locus_tag="PXD81_002126"
     CDS             1211..1687
                     /gene="recX"
                     /locus_tag="PXD81_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HDL5538113.1"
                     /translation="MTTSTTKTNKYTAVNYLLYLLSKRDYSEQELRQKLKQKEYELTE
                     IDAAIEKAQANQWQSDERFCTTFIRYRSMQGIGPRRLKQELKLKGVKDWLINQELENA
                     EVDWFELAEQIFEKKRPLVWDLKAKQKMWRFMVSRGFYNDHFSHLMALDYSSDEYE"
     gene            1680..1991
                     /locus_tag="PXD81_002127"
     CDS             1680..1991
                     /locus_tag="PXD81_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005724113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HDL5538114.1"
                     /translation="MNREKITFFSRFEADILSGKKTITIRDKSESYFQPNQELAVFTN
                     ETDRFFANIKVLSVTPIVFDELNEQHAKQENMSLAELKQVIREIYPNDNAFFVIEFTL
                     I"
     gene            2001..2537
                     /locus_tag="PXD81_002128"
     CDS             2001..2537
                     /locus_tag="PXD81_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
                     /protein_id="HDL5538115.1"
                     /translation="MVLFKSTKQTETCPQCGSPLQIKQGKKGLFLGCSAYPECDYIKP
                     LHQAGHIIKQLDELCPECGGNLQLKQGNYGIFIGCSHYPECHFIVHEEAEVEEEFDCP
                     ECKQHKLIARKGRSGKIFYGCIGFPNCKFTLATRPINHLCEKCGCELATIKKIKGKQF
                     TICANKSCQHQTELKPNE"
     gene            2530..3084
                     /locus_tag="PXD81_002129"
     CDS             2530..3084
                     /locus_tag="PXD81_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sua5/YciO/YrdC/YwlC family protein"
                     /protein_id="HDL5538116.1"
                     /translation="MNNFLEIVNQLKQEEVVAYPTEAVFGLGCNPQSEKAVRKLLVLK
                     QRPEEKGLILLASDLKYLLPYIDEARMTEQAWQRLSHIGEQAITWIVPVKNTVPSYLT
                     GKFDSIAVRLCNVPAVVQLCEASGVALTSTSANLSGEPPCRNAKEVIDQFGQDFPVLH
                     ADTLGRENPSEIRDIFSQQIFRQG"
     gene            3091..3915
                     /gene="aroE"
                     /locus_tag="PXD81_002130"
     CDS             3091..3915
                     /gene="aroE"
                     /locus_tag="PXD81_002130"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005615478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HDL5538117.1"
                     /translation="MNRYAVWGNPIAQSKSPRIHQLFALQTQRNIEYIANLGDETAFE
                     QQLADFFASGAKGCNITAPFKERAFKLADEFSETALLAQACNTLKRLEDGRLYADNTD
                     GAGLVSDLERLGWLQKGHKTLILGAGGATKGVLLPLLKAGLEITLYNRTQEKAVVLAE
                     KFAKFGKIQTACLSDLANQLFNLIVNATSLGLQGKYVELPESVIASANIYDMQYAPNM
                     QTPFLNYARRCGATACQDGLGMLVGQAAFAFSLWEGQFPDVEPVLAQLKLEMQTVK"
     gene            4082..4158
                     /locus_tag="PXD81_002131"
     tRNA            4082..4158
                     /locus_tag="PXD81_002131"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4116..4118,aa:Met,seq:cat)
     gene            4161..4245
                     /locus_tag="PXD81_002132"
     tRNA            4161..4245
                     /locus_tag="PXD81_002132"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4195..4197,aa:Leu,seq:tag)
BASE COUNT         1395 a          804 c          941 g         1184 t
ORIGIN      
        1 gcaacaagcg gttgctttta gataaaagtt tacaaaggat agaatatggc agagaaaaaa
       61 agtcaaaaga acactccggt taaacagatt gatccggaac aaaaagaaaa agcgttggca
      121 gccgcattag ctcaaattga aaagcaattt ggcaaaggct caattatgaa gttaggcgat
      181 actcaagccc ttgatattga agcagtttca accggttctc ttggtttaga ctcagcatta
      241 ggcattggtg gtttaccaat gggtcgtatt gtggaaattt atggaccgga gtcttcaggt
      301 aaaactacat taactttatc tgtggtggct caagcccaaa aaaatggtaa aacttgtgcc
      361 tttattgatg ccgaacacgc acttgatcct atttacgccc gtaagttagg ggtagataca
      421 gatggattat taatttcaca accggataat ggcgaacaag cattagaaat ttgtgatgcg
      481 ttagttcgtt ccggtgcggt tgatgtaatt attgtggact ctgttgcggc acttacacca
      541 aaagcagaga ttgaaggcga tatgggtgat tcccatatgg gcttacaagc tcgtttaatg
      601 tcacaagcat tgcgtaaatt aaccgcaaat atcaaagcga ccaactgctt ggtgattttc
      661 attaaccaaa ttcgtatgaa aattggagta atgttcggta accctgaaac cacaactggt
      721 ggtaatgccc ttaaattcta tgcttcagta cgtttagata ttcgtcgttc aggcgtagta
      781 aaagacggtg atgaggttat cggtagcgaa acaaaagtga aaattgtgaa aaacaaggtt
      841 gctccaccgt tccgtgaagt ccaatttgat attatgtacg gtgagggcat tgcccgtatg
      901 aatgaattac tcattttagc ggaatctcac ggcttcatta ataaagcagg ggcttggttc
      961 tcttatgaag gggagaaaat cggtcagggt aaaaataatg ccatcaaatg gttaaaagaa
     1021 caccctgaag tggcgagtaa aattgagcaa gatattcgaa atctactcat ttcaaaccca
     1081 acttttacgg ctactccaga ttctgaaaat gcagacaatg cagatgatga atttagtgaa
     1141 gaagaactct aattaaattt atgcggtttt cgctcaacgt gaaaatcgca tttttattta
     1201 taaccgataa atgacaacat ctactactaa aacaaataaa tacacagcgg ttaattatct
     1261 gctatattta ctttcaaagc gtgattacag cgagcaagag cttcggcaaa aactaaagca
     1321 aaaagagtat gaactcacgg agattgatgc agcgattgaa aaagctcaag cgaatcagtg
     1381 gcaaagtgat gaacgctttt gtaccacttt tatccgttat cgttcaatgc aaggcattgg
     1441 gcctcgccgt ttaaagcaag agctaaaact taaaggggta aaagattggt taattaacca
     1501 agaattagaa aacgccgaag tggattggtt tgaattagct gagcaaattt ttgagaaaaa
     1561 acgaccgctt gtatgggatc tgaaagcaaa gcagaaaatg tggcgattta tggttagccg
     1621 tggtttttat aacgatcatt ttagtcattt aatggctctg gattacagta gcgatgaata
     1681 tgaatagaga gaaaattacc tttttcagcc gatttgaagc cgatatttta agcggtaaaa
     1741 aaacaattac gattcgagac aagtcagaat cttattttca accaaaccaa gaattagcgg
     1801 tttttactaa tgaaaccgat agatttttcg ccaatattaa agtgctatcg gtaacaccta
     1861 tagtgtttga tgaattaaac gagcaacacg ctaaacaaga aaatatgagc ttagccgaat
     1921 tgaaacaagt tattcgggag atttatccga atgacaatgc gttttttgtg attgaattta
     1981 cgcttattta gcaaatcaat atggtacttt tcaaatcaac caaacagacc gaaacttgcc
     2041 cacaatgtgg ctcgccgttg caaattaagc agggcaaaaa agggttattt ttgggttgtt
     2101 cagcttaccc tgaatgtgat tatattaagc cattacatca agccggtcat attattaagc
     2161 aattagatga actttgccct gaatgtggcg gaaacctcca attaaaacaa ggcaattatg
     2221 gtatttttat cggctgtagc cattaccctg aatgccactt catcgtacac gaagaagctg
     2281 aggttgaaga agagtttgat tgccctgaat gcaagcaaca taaactgatt gctagaaaag
     2341 ggcgttcagg caaaatattt tatggttgta taggcttccc taattgcaaa tttacattag
     2401 caactcgacc gattaaccac ttgtgtgaaa aatgtggttg tgaactcgct acaatcaaaa
     2461 aaattaaggg caagcaattc accatttgtg ctaacaaaag ttgccagcac caaacagaat
     2521 taaaaccgaa tgaataattt tttagaaata gttaatcaat taaagcaaga agaagttgta
     2581 gcttacccaa ccgaagcggt ttttggctta ggctgcaatc cacaaagtga aaaagcggta
     2641 aggaaattac tcgtattaaa acaacgccct gaggaaaaag ggctaatttt actggcttct
     2701 gatttaaagt atcttcttcc ctatattgat gaagctcgaa tgacggaaca ggcttggcaa
     2761 cgcctaagcc atattggcga gcaggcaatt acttggatcg taccggttaa aaatactgtg
     2821 ccaagttacc tcaccggcaa atttgattct attgccgtac gtttatgtaa tgtgccggcg
     2881 gtggttcagt tgtgtgaggc aagcggcgtt gcgttgacct ctaccagtgc caatttgagc
     2941 ggagaaccac cttgccgcaa tgcaaaagaa gtcatcgatc agtttggtca agattttcct
     3001 gtattacacg cagacacgct aggtagagaa aacccttccg aaattcgaga tattttcagc
     3061 caacagattt ttagacaagg ataagccgaa atgaaccgat atgccgtgtg gggcaacccg
     3121 attgctcaaa gtaaatcccc ccgtattcat caactctttg ctttacaaac ccaacgcaac
     3181 attgaatata tcgctaactt aggagatgaa acggcttttg agcaacaatt agccgatttt
     3241 ttcgcttctg gagcaaaagg ttgcaacatc accgcccctt ttaaagaaag agcctttaaa
     3301 ttggcagatg aatttagcga aaccgctttg ttggcacagg cgtgtaatac gcttaaacgc
     3361 ttagaagatg gacggttata tgccgataat accgacgggg ccggtttagt ttctgatttg
     3421 gaacggctag gttggttaca aaaagggcat aaaaccttaa ttcttggagc aggtggagca
     3481 accaaaggcg tattacttcc gttacttaaa gcggggttag agattactct atataaccgc
     3541 acgcaggaga aagcggtcgt tttagccgaa aaatttgcaa aatttggcaa aattcagacc
     3601 gcttgtttaa gcgatttggc aaaccaactg tttaatttaa tcgtcaatgc cacctctctc
     3661 ggtctgcaag gcaaatatgt tgaattgcct gaatctgtta tagcatcggc gaatatttac
     3721 gatatgcaat atgcccccaa tatgcaaact ccttttctca attatgcaag gcgttgtgga
     3781 gcaacagcgt gccaagacgg attaggaatg ttggttggac aggcagcgtt tgcgttttct
     3841 ttgtgggaag ggcaattccc cgatgttgag cctgtactgg ctcaattaaa attagaaatg
     3901 caaacggtga aatagcagaa tataagctat tattttataa acaaaaaatg ctagatttaa
     3961 gccaaaacga cgaataacta tacaagcgaa tcaaaaattc aaatttttta aaaaaagtat
     4021 ttgacggaaa gccctaaaat cagcataatg cactccgttg accgatatgg tgaacgcaaa
     4081 tggctacata gctcagctgg ttagagcaca acactcataa tgttggggtc gcaagttcga
     4141 atctcgctgt agccaccatt gcgggactgg cgaaattggt agacgcacca gatttaggtt
     4201 ctggcgccgc gaggtgtgtg ggttcaagtc cctcgtcccg caccatttat cgattaacaa
     4261 aaattagtgt tggggtatcg ccaagcggta aggcaccggg ttttgatctc ggcatcccta
     4321 ggtt
//
LOCUS       DANLYI010000186         4310 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.187, whole genome shotgun sequence.
ACCESSION   DANLYI010000186 DANLYI010000000
VERSION     DANLYI010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4310
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.187"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            complement(86..3376)
                     /gene="recC"
                     /locus_tag="PXD81_002133"
     CDS             complement(86..3376)
                     /gene="recC"
                     /locus_tag="PXD81_002133"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HDL5538118.1"
                     /translation="MFTLYQSNQISSLAEMLVKIQQVNPLEDPFEPETILIQSQGMAQ
                     WLQMQIAELNGVMGNCDFLYPTTFLWQQYRLLFPELPKENIFERSSLVWRIMRLLPSC
                     LTLSEFAPLKRYLGDQPNKEQYQLKLYQLSAKIAELFDQYLVYRPHWLVSWEEGNLQA
                     IENEIKIATAAKPIDFDLIRADMKWQSHLWQQIVSDLQSESDEQVFTTSHRAYLQQNY
                     FAKLDDLTETEKAKLPKRIFIFGISSFSPLQLAVFKKLSEHCDIHLFFFNPSEKYWGD
                     SVEEKVWQKLALSHKISQDDLDNLLAEQRNKLLNLWGKQGRDFLAQLIEFEPNTMDVF
                     VEPEENSNLNKLKKQIFSLENDATITDLTADNSLQIHSCHSPMREVEVLHNQLLNLFE
                     QDSTLLPKDIIVMSPDINKYAPYIDAVFSRYNTGNRDPRYIPFSLSDQAFTQVDPIIH
                     SFLQLLAMKESPFNAEELFDLLEVNAIQTRFGFSTEDIHTLRVWVRNAGVRSGVEIEQ
                     PHWQNYNSWENGLNRLLLGSSLKEEQGIWENTLAFGDSYGLNAELVGRLASFIEALLA
                     WVNFLQTPQPLAAWQQAIKNLTADLYQENAENAASILLLNNVNDQIIEQISATHFSDD
                     LQIEVLALLFEQQLSQHSQQLNFLVGRVNFATLLPMRAIPFKVICLLGMNEADFPRQQ
                     QINSFDLMQYAPKKGDRAKRDDDRYLFLEALLSAQQMFYISYIGQSQKDGKEMLPSIL
                     VSQLLDAMNEALTPECKQQLSDEVEVLVRKQPLSVFSKKNFTHPRDHAYNAEWIIKPT
                     VAVQDFLSEPLTNESVASLELDELISFVQNPIRFFFLKVLGISFNTYDDTIDESEIFE
                     MSGLDNYRILNELLTINLAQQQRFFAEEQLKGNLPASYFAQIATQDLIGKITEMQAAL
                     SHYLASESRILSVNREVELERQTLTLNGNIANLYQNEVVLWRVGHLRDKDKIQTWIYH
                     LLLCAYFPEKTVKFYYREGEQVGVLSFETVSQIEALSQLSIYLNAYLDGLSQAQLVIY
                     DRIEEYLKGDGTNSEQVLIDLAEKDERDGSYLHRLLAQTKELDSQAIHQRTQSWFSLM
                     NQKIAKGD"
     gene            complement(3425..3718)
                     /locus_tag="PXD81_002134"
     CDS             complement(3425..3718)
                     /locus_tag="PXD81_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5374 domain-containing protein"
                     /protein_id="HDL5538119.1"
                     /translation="MIKYKAETALSLLVAITLFAIVVLSFSQWQSAQNHRLNQHFQQQ
                     QAVMILENQLALKLAGLDCESSITQNQIRYQIQCSGNRLTIRFPLGKIELNND"
     gene            complement(3711..>4310)
                     /locus_tag="PXD81_002135"
     CDS             complement(3711..>4310)
                     /locus_tag="PXD81_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2572 family protein"
                     /protein_id="HDL5538120.1"
                     /translation="EKWLGQQNITHFYREKYLSDQYNFLALYPQDKTQICQKFQKEQI
                     SQADLSDKQFRFLQYQFACQFHSLFKDKKPTKEKYIHFTHLADYLDLSNVPNQEIYTI
                     HSLAELPSSSEREPKIVLAKNAINENLPQEFYGIVITDYLFDITGKKMYGTLYSSYDN
                     AREERNLTFKKEVLAELEARYSAWRYLPYSEHLMGAVDD"
BASE COUNT         1218 a          929 c          855 g         1308 t
ORIGIN      
        1 ggaaaaaatt ctatttttat aggtaggcga attattattc gccttctcat tttgcaaatt
       61 ttcagccaaa aacgaccgct tgtagttaat cgcctttcgc tattttctga ttcattaagc
      121 taaaccagct ttgagtacgt tggtgaatcg cttgagaatc cagctcttta gtttgagcga
      181 gaagccgatg gagatagctg ccgtctcgct catctttctc tgctaaatcg ataagaactt
      241 gttcgctgtt tgtgccatca cctttgagat attcctcaat tcggtcgtag atcactaact
      301 gggcttgcga taatccgtcc aaataagcat tcaaataaat gcttaattgc gataatgcct
      361 ctatctgcga gacggtttca aagctcaaca caccaacttg ctcaccctct cggtaataga
      421 atttcaccgt tttttcaggg aaataggcac acaataataa atggtagatc caagtctgaa
      481 tcttgtcttt atctcgtaaa tgccctactc gccatagcac cacttcattc tgatataggt
      541 tggcaatatt gccgtttagg gtgagggttt ggcgttccag ctcaacctcc cgattaacac
      601 tcaaaatacg gctttcgctg gctaaatagt ggcttaacgc cgcttgcatt tcggtaattt
      661 tgccgatcaa atcttgtgtg gcaatttgag caaaataaga agcaggtaaa ttgcctttga
      721 gttgctcttc ggcaaaaaaa cgttgctgtt gtgctaaatt tatcgttaat aattcgttta
      781 aaatccggta gttatccagc cccgacatct caaaaatttc gctttcatca atggtgtcat
      841 cataagtgtt aaacgaaatc cccaacactt ttagaaagaa aaacctaatc gggttttgta
      901 caaaagagat taattcatca agttctagcg aagccacgct ttcattggtt aaaggctcac
      961 taagaaaatc ttgtacggca acagtcggct tgataatcca ctctgcgtta taagcgtgat
     1021 ctcgtgggtg ggtaaaattc tttttgctaa acacacttaa aggttgtttt ctgaccaaaa
     1081 cttccacttc atcgcttaat tgttgcttgc attcaggcgt taaagcttca ttcattgcat
     1141 ctaacagttg ggaaaccaaa atcgacggta gcatctcttt gccgtctttt tgcgactgcc
     1201 cgatatagct gatgtaaaac atttgctgag cggaaagtaa ggcttctaag aacaagtagc
     1261 ggtcatcatc acgtttggca cggtctccct ttttcggggc gtactgcatt aaatcaaagc
     1321 tattgatttg ttgttggcga gggaaatctg cctcattcat acccagcaag caaattacct
     1381 taaacggaat cgcccgcatc ggaagcaagg tggcaaaatt cacccgtccg accaagaaat
     1441 taagctgctg tgaatgttgg ctgagctgct gctcaaacaa taaggctaaa acctcaattt
     1501 gcagatcgtc tgaaaaatgg gttgcggaaa tttgctcgat aatctgatca ttcacattat
     1561 tcaacaataa aattgatgca gcattttcgg cattttcttg atacagatca gcagtcaaat
     1621 tttttattgc ctgctgccac gctgccaacg gctgtggcgt ttgcagaaag tttacccacg
     1681 ctaataaagc ctcaataaaa gaggcaagtc gccccaccag ctcggcattt aagccatagc
     1741 tatccccaaa ggcaagggtg ttttcccaaa tcccctgctc ttctttcagg cttgagccaa
     1801 gcaataaacg gtttagcccg ttttcccacg aattgtagtt ttgccagtga ggttgctcga
     1861 tttctacacc cgaacgtacg ccggcatttc tcacccaaac ccgcagggta tgaatatctt
     1921 ccgtactaaa gccaaaacgg gtttggatcg cattcacttc cagtaaatca aacagttctt
     1981 cggcattaaa cgggctttct ttcattgcca gcagttgcag gaaactgtgg ataatcggat
     2041 caacctgcgt aaatgcttgg tcggataatg aaaaaggaat atagcgagga tcccgattac
     2101 ccgtattgta gcgagaaaac accgcatcaa tgtaaggagc gtatttatta atatccggcg
     2161 acatcacaat aatatctttc ggcagcaaag tgctatcttg ctcaaacaaa ttcaggagct
     2221 ggttatgcaa cacttccact tcccgcattg ggctgtggca cgaatgaatt tggagtgaat
     2281 tatcggcggt taaatcggta atagtggcgt cattttctaa gctgaaaatc tgttttttga
     2341 gtttattgag gttgctgttt tcctctggtt cgacaaagac atccatggta ttcggctcaa
     2401 attcgattaa ttgagctaag aaatcccgcc cttgtttgcc ccaaagattc agtagtttat
     2461 ttctttgctc cgccaataag ttatccagat cgtcttgcga aatcttgtgg cttaacgcca
     2521 atttttgcca cactttttct tccacactgt cgccccaata tttttcgctt gggttaaaaa
     2581 agaacaggtg aatatcgcaa tgctcgctga gtttcttaaa caccgctaac tgtaatggcg
     2641 agaaggacga aatcccaaaa atgaaaatgc gttttggcaa tttggctttt tccgtttcag
     2701 taaggtcatc taacttagcg aagtaatttt gttgcaaata ggcacggtgg gaggtagtaa
     2761 aaacctgctc atcagattcc gattgcagat cagatacaat ctgttgccat aaatggcttt
     2821 gccatttcat atcggctcga attaagtcaa aatcaatcgg cttagcggca gttgcaattt
     2881 ttatctcatt ttcgatcgct tgtaagttgc cctcttccca gctaaccagc caatgggggc
     2941 gatacaccaa atattggtcg aacaattcgg caattttggc agaaagttga tagagcttaa
     3001 gctgatactg ttctttatta ggttgatcac ctaaataacg ctttagcgga gcaaactccg
     3061 atagtgtcaa gcaactcggc aataaccgca taatccgcca aaccaacgaa ctacgctcaa
     3121 aaatattctc tttcggcagc tccgggaaca acagccgata ctgttgccat aaaaatgtag
     3181 tagggtagag aaaatcgcaa ttccccatca caccgtttaa ttcagcaatc tgcatttgta
     3241 gccactgtgc cattccctga ctttggatca aaatggtttc cggctcaaac ggatcttcaa
     3301 gtgggtttac ctgctggatt ttcaccagca tctccgccag tgatgaaatt tggttggatt
     3361 ggtaaagtgt aaacataata acgcctcgct aattgaatta ttgtcctcca aagcgaggga
     3421 cgattcaatc attattcagc tcaattttgc ctaacggaaa gcgaatggtt aagcgattgc
     3481 cagagcattg aatctgatag cgaatctgat tttgagtaat acttgattca caatctaacc
     3541 cggctaattt gagggcaagc tgattttcta aaatcataac cgcttgttgc tgttggaaat
     3601 gttgatttag cctgtggttt tgtgccgatt gccactggct gaaactcaac accacaatcg
     3661 caaatagcgt gatggcaacc aaaagcgaca aagccgtttc tgctttatat ttaatcatca
     3721 accgctccca tcaaatgttc ggaatatggc aaatatcgcc aagcggaata cctcgcctca
     3781 agctctgcca gtacctcttt tttaaacgtt aaatttcgct cctctcgggc attatcataa
     3841 gaggaataaa gcgtgccgta catttttttg ccggtaatat caaacagata gtcggttatc
     3901 acaatgccgt aaaactcttg cggtaagttt tcattaattg cgtttttagc aagcacaatt
     3961 ttaggctcac gttcactgct actaggtaat tcagctaaag aatgaatagt ataaatctct
     4021 tggtttggca catttgataa atctaaataa tccgccaaat gggtaaagtg aatatatttc
     4081 tctttagtcg gttttttatc tttaaataaa ctatggaatt ggcaggcaaa ttgatactgt
     4141 aaaaacctga attgtttatc ggataaatcc gcttggctga tctgctcttt ttgaaatttt
     4201 tggcatattt gcgttttatc ttgtggatat aacgctaaaa aattatattg atcggaaagg
     4261 tatttctctc tataaaaatg ggtaatgttt tgttgtccaa gccatttttc
//
LOCUS       DANLYI010000187         4301 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.188, whole genome shotgun sequence.
ACCESSION   DANLYI010000187 DANLYI010000000
VERSION     DANLYI010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4301
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.188"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            complement(3..1409)
                     /locus_tag="PXD81_002136"
     CDS             complement(3..1409)
                     /locus_tag="PXD81_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HDL5538121.1"
                     /translation="MKLTKIVLSVVAVIGAVAVGGSWYTGKQVEEKYQELVVQANNQL
                     KQLSGEFGANIELKDVQIDRHFFISNAKYRLEVDLGNGKKLDFVGNDKLHHGPLPLNR
                     LAKLNLAPVMMSAESKLQAPEALKKLMGEHIGTGFSNISYSGDVEGHFDISPIKHSDE
                     TGHIETTPIKVQYSYDQKLKGVDSTVELESLKIKAEEFDFQIQGIVYDVQTENNESYP
                     NLTLGKGSGKINAIEFKSKDGKLSQIKDVVVKGDNLLKGDRLISTGDLEAATFNIEGV
                     EMGKLKMDMAYDLDAKLTNDITPLLSNPQTLENEKTGELLLQLLAKSFKFHINNFSLE
                     NSKGKVDLALLLNMAQFDPQNLGNMQAVLQALSTSKFTSNINRQYAEDIIRQVSIVTE
                     KLGEEEAKAFAKQQVDAVFLNAGVEQYGLRKVDDNNVKIELTIDNGKVNLNGRELTED
                     ELQMALFMIVMGAGSLGQ"
     gene            complement(1459..2265)
                     /gene="trpA"
                     /locus_tag="PXD81_002137"
     CDS             complement(1459..2265)
                     /gene="trpA"
                     /locus_tag="PXD81_002137"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005603844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HDL5538122.1"
                     /translation="MSRFDTLFTALKAKNEGAFVPFVTLCDPGFERSFEIIDTMIKNG
                     ADALELGFPFSDPLLDGPVIQAANKRALDGGYSTDSCFELIAKIRAKYPDIPIGLLLC
                     ANLIFVPTAEVFFQRCQQSGVDAVLIADLPVFAAQEFVPTAEKYGIQPVFICPPNADQ
                     NTIERIAQLTQGYTYLVSRAGVTSAENQAHASNLDNLVESLKQAGAAPILQGFGIAKP
                     EQVKEALRLGADGAISGSAIVKIIERNLADQANLLKELAEFVQVMKAATK"
     gene            complement(2267..3457)
                     /gene="trpB"
                     /locus_tag="PXD81_002138"
     CDS             complement(2267..3457)
                     /gene="trpB"
                     /locus_tag="PXD81_002138"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HDL5538123.1"
                     /translation="MSDTLLNPYFGEFGGMYVPEILVPVLKELEQAFVDAQQDPTFQA
                     EFADLLKNYAGRPTALTLCRNLTKGSKTKLYLKREDLLHGGAHKTNQVLGQILLAKRM
                     GKTRIIAETGAGQHGVATALACAMLGMPCVVYMGAKDVERQSPNVFRMRLMGAEVIPV
                     QKGSCSLKDACCEAMRDWAANYDTTHYLIGTAAGPHPFPTMVREFQKMIGEEIKRQIL
                     AKEDRLPDAVIAAVGGGSNAIGMFADFIEEQSVKLIGVEPAGKGIETGEHGAPLKHGT
                     TGIYFGMKSPIMQDKDGQIEESYSISAGLDFPSVGPQHAYLNATGRAEYVSITDAEAL
                     EAFQALAKNEGIIPALESSHALAYALKMIKQNPEKEQLLVVNLSGRGDKDIFTVDKIL
                     NGGE"
     gene            complement(3469..4221)
                     /locus_tag="PXD81_002139"
     CDS             complement(3469..4221)
                     /locus_tag="PXD81_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDL5538124.1"
                     /translation="MKIALVTGVTAGFGLAICKLLLQSGYKVIGTGRRAERLEELHLQ
                     FGDKFLPLAFDIQQPDEVEKALNSRPKAWQQIDLLVNNAGLALGLEPAQKADLNDWYQ
                     MIDTNIKGLVTMTRLVLPEMVERKSGHIINIGSIAGTYPYPGGNVYGGTKAFVKQFSL
                     NLRADLAGTKVRVSNVEPGLCGGTEFSNVRFKGDDEKAAKVYENVQFVRPEDVANIVL
                     WLNQQPEHVNINRVEVMPVAQTSSALNVARNI"
BASE COUNT         1200 a          995 c          760 g         1346 t
ORIGIN      
        1 gattattgcc cgagtgagcc tgctcccatc acaatcataa acagagccat ttgaagttca
       61 tcttcggtca gttcacgtcc gtttaaattc acttttccgt tatctatagt taattcaatc
      121 ttcacattgt tgtcatctac ttttcttaat ccgtactgtt caactcccgc attgagaaaa
      181 acagcatcaa cttgttgctt ggcaaatgct ttcgcctctt cttcgcctaa tttttctgta
      241 actattgaga cttggcgaat aatatcttcc gcatattgac ggttgatatt tgaagtgaat
      301 ttacttgttg aaagggcttg caataccgct tgcatattac caagattttg tgggtcaaat
      361 tgagccatat tcagtaataa agccaaatca actttgcctt tgctgttttc aagtgagaaa
      421 ttattaatat ggaatttaaa tgattttgct aatagttgta ataataactc gcctgttttt
      481 tcattttcta atgtttgagg attagataat aaaggcgtaa tatcattggt taatttagca
      541 tctaaatcgt atgccatatc catttttaat ttacccattt caacaccctc aatgttgaaa
      601 gtggctgcct ctaaatctcc ggtagaaatt aaacgatcgc cttttaatag attatcgccc
      661 ttcaccacaa catctttaat ttgagataat ttgccatctt tagacttaaa ctcaatagcg
      721 ttaatttttc cactaccttt gcctaaagtt aaattaggat aactttcatt attttctgtt
      781 tgaacatcat aaactatacc ttgaatttga aaatcaaatt cttctgcttt tatcttaaga
      841 ctttctagct caacagtgct atcaacccct tttaattttt ggtcgtagct atattgcact
      901 ttgattggcg tggtttcaat atgacctgtt tcatcactat gtttaatcgg ggaaatatca
      961 aaatgccctt ccacatcgcc cgaatagctg atattagaaa aaccggtgcc aatatgttca
     1021 cccatcaatt ttttaagggc ttcaggagct tgtaatttgc tttccgcact catcattaca
     1081 ggagctaaat taagtttcgc taaacgatta agcggtagcg gaccgtgatg aagtttatca
     1141 ttaccaacaa agtccaattt cttaccatta cccaaatcca cttctaaacg atatttcgca
     1201 ttagagataa agaaatggcg atcgatctgc acatctttaa gctcaatatt cgcaccaaat
     1261 tcgccactca gttgtttaag ttggttatta gcttgaacaa ccaattcttg atatttttct
     1321 tcaacctgct tacctgtgta ccaacttccg ccaactgcaa cagcaccaat taccgctaca
     1381 actgaaagaa caatttttgt taatttcatt ctgtttcctt ttattttcaa aaaaatttaa
     1441 aaaatgaccg cttgtagttt acttcgtagc cgctttcatc acttgtacaa attcggcaag
     1501 ttctttgagc aaattagctt ggtcagctaa attacgttca ataattttca caatcgctga
     1561 gcctgaaatc gccccgtctg ccccaagcct taacgcctct tttacctgtt caggtttggc
     1621 tatgccaaaa ccttgtaaga taggggcagc tccggcttgt tttaaacttt ccactaaatt
     1681 atccaaatta cttgcgtgag cttggttttc agcactagtt acaccggcac gggaaactaa
     1741 ataagtgtag ccttgggtta attgagcaat ccgttcaatg gtgttttgat cagcattcgg
     1801 cgggcaaata aacaccggct gaattccata tttttcagcg gttggcacaa actcttgagc
     1861 agcgaaaaca ggcaaatcag caatcaacac cgcatcaact ccactttgtt ggcaacgttg
     1921 gaaaaatact tctgcggttg gcacaaaaat taaattggca cacagtagta aaccgatagg
     1981 aatgtctgga tatttagctc gaattttggc aatcagctca aaacagctat cagtgctata
     2041 gccaccatct aaagctcgtt tattcgctgc ttgaatcacc ggcccgtcta agagggggtc
     2101 agaaaacgga aagcctagct ctaaagcatc agccccgttt ttaatcatcg tgtcgataat
     2161 ctcaaaactg cgttcaaaac caggatcgca taatgtcaca aacggcacaa aagccccttc
     2221 gttttttgct tttaatgcag taaatagcgt atcaaagcgg ctcattttac tctcctccat
     2281 ttaaaatttt atccacggtg aaaatgtctt tatcgcctcg acccgataaa ttcaccacta
     2341 ataactgctc tttttcaggg ttctgcttaa tcatttttaa cgcataggct aaggcgtggg
     2401 aagattctaa cgccggaata atcccctcat ttttggctaa tgcttggaat gcctctaaag
     2461 cctctgcatc ggtaatcgat acatattcag ctcgccctgt cgcatttaaa taagcgtgtt
     2521 gcggcccgac agaagggaaa tctaagccgg ctgaaatgga gtaagactct tcaatttgcc
     2581 cgtctttatc ctgcataatc ggcgatttca taccgaaata gatccccgtt gtgccgtgtt
     2641 tgagcggtgc accgtgttca ccggtttcaa ttccttttcc tgccggctca acaccgatca
     2701 atttgacgga ttgctcttcg ataaaatctg caaacatacc gatagcattt gagccaccgc
     2761 ctacggcagc aatcaccgca tcaggcaagc ggtcttcttt tgctaaaatt tgacgtttga
     2821 tctcttcgcc aatcattttc tgaaattcac gcaccattgt cgggaacggg tgcgggccgg
     2881 ctgcagtgcc gattaggtaa tgggtagtat cataatttgc cgcccaatcc cgcattgcct
     2941 cgcaacaggc atctttgagc gagcaagagc ctttttgtac cggaatcacc tccgctccca
     3001 ttaaacgcat acggaataca tttggcgatt ggcgttctac atcttttgcg cccatataca
     3061 ccacgcaagg cattcctaac atcgcacacg ctaatgcggt tgccacaccg tgctgtcctg
     3121 ctcctgtttc ggcaataatg cgggttttac ccattcgttt agcgagtaag atttgcccta
     3181 gcacttggtt ggttttatgt gccccaccgt gtaaaagatc ttcacgtttt aaatagagtt
     3241 tggttttact gcctttggtt aaattacgac aaagggttaa agcagtcggg cgaccggcat
     3301 agtttttgag taaatcggca aattcagctt ggaatgttgg gtcttgctga gcatctacaa
     3361 aggcttgttc taattctttt agcaccggca ctaaaatttc cggcacatac ataccaccaa
     3421 attcaccaaa ataaggatta agaagtgtgt ctgacataat atttcctttt aaatatttct
     3481 cgctacattt aaggcagaag aagtttgagc caccggcatc acttcaaccc gattaatatt
     3541 aacgtgttca ggttgctgat tcagccaaag cacaatattt gccacatctt ccgggcgcac
     3601 aaactgcacg ttttcataaa ctttagccgc tttttcatca tcgcctttaa agcgtacatt
     3661 tgaaaattcc gtaccgccac atagccccgg ctcaacattg gaaacacgca ctttagtgcc
     3721 ggctaaatct gcccgcaaat ttaggctaaa ctgtttcacg aaggctttag taccaccata
     3781 tacattaccg cccgggtaag ggtaagtgcc ggcaatcgag ccgatattaa taatatgccc
     3841 acttttccgc tccaccattt caggcaaaac taagcgtgtc atcgttacca atcctttaat
     3901 attggtatcg atcatttgat accaatcgtt caaatctgct ttctgtgccg gttctaagcc
     3961 gagtgcaagg cctgcattgt ttactaataa atcaatttgt tgccacgctt tagggcggct
     4021 attcagtgct ttttccacct catcgggctg ttgaatatca aatgcaagcg gtagaaattt
     4081 atcgccaaat tgtaaatgca gttcttctaa acgctctgcc cgtctgcccg tgccaattac
     4141 tttataaccc gattgaagca ataatttaca aattgccaac ccaaatccag cagttactcc
     4201 tgtgactaat gctattttca tcttttcacc ctcaaattta aaatgtacta ttaaagtagt
     4261 acaaaaaaat taaaattgca agaaataata ttgccataaa a
//
LOCUS       DANLYI010000188         4230 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.189, whole genome shotgun sequence.
ACCESSION   DANLYI010000188 DANLYI010000000
VERSION     DANLYI010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4230
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.189"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            complement(28..336)
                     /locus_tag="PXD81_002140"
     CDS             complement(28..336)
                     /locus_tag="PXD81_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HDL5538125.1"
                     /translation="MKKYIATMLLAFGLVHSSYAQEKTAQIETKQAEKTVVLHIPEMY
                     CQLCVYLVNKELRAVEGVISTKASMKERKATVVVKESVDNQALIQAVGNLKYTAKVIE
                     "
     gene            complement(338..706)
                     /locus_tag="PXD81_002141"
     CDS             complement(338..706)
                     /locus_tag="PXD81_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5538126.1"
                     /translation="MNSSLKSYNHKFITMCVTAIVSAVTSTFCCIGPLVYLVFGISSP
                     WLMELNQLEFLQLPMLLISLAAFGYGFWLLNFSNKIICTKYFSRRTLVILYWVVSVVV
                     MFFLTYPYVLPYILEMWSGE"
     gene            complement(681..1787)
                     /locus_tag="PXD81_002142"
     CDS             complement(681..1787)
                     /locus_tag="PXD81_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005647785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HDL5538127.1"
                     /translation="MNKLIASLVASSCATAFAAPVQQNNFHTTTHTYEFTQSYDLVVP
                     QGSKGTTNLWIPLPSDTDYQTVKSIEFEGSYKNAYITENNAYGAKTLFATWDTDAPKR
                     DLKVKMVIQTQDREPMAQGALANYQMPEKITYSVDVLEYLKPTAHIKTDGIVKEYADK
                     IVGNEQNPLKKAELIHKWIVDNMERDNSVLGCGDGDVEKILTTKVLKGKCTDINSVFV
                     ALARASNIPAREVFGIRLGQAVKMGQYSKTAFGSSKDGVANESGGQHCRAEFYLAGFG
                     WVPVDSADVAKMRLAEKKDVDDADVQAVSKYLFGNWEMNWVGFNHGRDFDLYPQPELT
                     PLNNFGYPYAEVGGDPLNSFEAKEFGYEFISKEL"
     gene            complement(2027..3070)
                     /locus_tag="PXD81_002143"
     CDS             complement(2027..3070)
                     /locus_tag="PXD81_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDL5538128.1"
                     /translation="MQPINIAIAGAGFSSRVFHIPFLKQDARFNIVKVYERTSNNAAE
                     WLPNAETVRDFNALLTPEVDLVLITTPNQTHYAMVKESLLAGKHVLVEKPLVATVAEA
                     EELAELAKRQGKVLYVYQNRRWDSHIATAKSLMEQNLLGELVDCEIRFDRYAKEKNVK
                     VWKESGELGTGLVYDLGVHLIDQALYLFGKPEAVYADVRYQHEGALVDDNFDIQLYYA
                     NGFKVVLKASRYAREPSPTFVLHGKLGSYLKQTPDNQEDLLKNGVQPIGKDWNIEEKE
                     NWGILHTEVEGNVVRQPYRNAEVSYQNLFDDLYQAIAHNAEPIIKLEDVIFVLKVIES
                     VFESAKLGQKIYL"
     gene            complement(3174..3458)
                     /locus_tag="PXD81_002144"
     CDS             complement(3174..3458)
                     /locus_tag="PXD81_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5538129.1"
                     /translation="MRYWLISLSILLSGCVVPVTPTGQAGIPMPIIIGVSDPLDSKEV
                     HICTLKPFTQEYRSEHSSRGRAKLNVQKQCRANHSEMFCEEKDIVCKTYD"
     gene            complement(3488..3673)
                     /locus_tag="PXD81_002145"
     CDS             complement(3488..3673)
                     /locus_tag="PXD81_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HDL5538130.1"
                     /translation="MKCRQATELISQSCDEKLRLQQHMRLQLHLLVCPKCRNFNKNTK
                     KLGKILRAYCNDKDAPF"
     gene            complement(3670..4191)
                     /locus_tag="PXD81_002146"
     CDS             complement(3670..4191)
                     /locus_tag="PXD81_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDL5538131.1"
                     /translation="MVKFATLQLADKDLAEDVVQDALTNAYKHSASFRREAALKTWIF
                     AILKNKILDLLRTRKRHIPVSSLCEEEESPSAFFDRHDHWLEHQDVTEWRGVAQSTYQ
                     KEFWAIFDICLNKLPAQQARVFMMREHLEMSSAEICQECEISLSNLNVLLHRARLRLQ
                     ECLSRNWFREESK"
BASE COUNT         1221 a          929 c          792 g         1288 t
ORIGIN      
        1 tcaaacaata atgtttgagc tttggtttta ttcaatgact tttgcagtat atttgaggtt
       61 tccgaccgct tgtatcaggg cttggttatc cacactttct tttaccacca cagtcgcttt
      121 tcgctctttc attgaggctt tggtggaaat cacaccttct actgctcgta gttctttatt
      181 gactaaataa acgcagagct ggcagtacat ttccggaata tggaggacga cggttttctc
      241 tgcttgtttc gtttcaattt gagctgtttt ttcttgtgcg tagcttgaat gaaccaaacc
      301 aaaagccaac agcatcgttg cgatatattt tttcatttta ttctccgctc cacatttcca
      361 aaatgtaagg caatacataa gggtaagtta aaaagaacat caccaccaca gaaactaccc
      421 aatacaaaat gaccagggtt ctgcgggaga agtatttggt gcagatgatt ttgttggaaa
      481 aattcagcaa ccagaagcca tagccaaacg cagcgagcga aatcagcaac atcggcagtt
      541 gtagaaactc cagttggttt agctccatca accaagggga agagatgcca aacactaaat
      601 aaaccagtgg cccaatacaa cagaatgtgg aggtcaccgc cgacactatg gcggtcacgc
      661 acattgtgat aaatttatgg ttataactct ttagagatga attcatagcc aaattctttc
      721 gcctcaaacg agtttaacgg gtcaccacct acctcagcat aagggtagcc aaagttattc
      781 agtggtgtaa gttccggttg tggatataaa tcgaaatcac gtccgtgatt gaagcctacc
      841 cagttcattt cccagttgcc gaataagtat ttcgagaccg cttgaacatc agcatcatcc
      901 acgtcttttt tctcggctaa acgcatttta gcgacatcgg cagaatctac aggcacccag
      961 ccgaagcctg ctaaatagaa ctctgcacgg cagtgctgcc cgccgctttc attcgccacg
     1021 ccatcttttg agctaccaaa tgcggttttg gaatattgcc ccatttttac cgcttgccct
     1081 aaacgaatac caaacacttc acgagcagga atgttagagg cacgagcaag tgctacaaac
     1141 accgagttga tgtcggtaca tttacctttt agcactttgg tggtgagaat tttctccaca
     1201 tcgccatcgc cacagcctag caccgagtta tcacgttcca tattatcaac aatccacttg
     1261 tggataagtt ccgctttttt caacggattt tgctcgttac ccacaatttt atcggcatat
     1321 tctttcacaa taccgtctgt tttaatgtga gccgttggtt ttaaatactc caatacatca
     1381 acagaataag tgattttctc cggcatttgg taatttgcca acgccccttg agccataggc
     1441 tcacgatctt gggtttggat caccattttc acttttagat cacgctttgg tgcatcagta
     1501 tcccaagtgg caaacaaggt ttttgcacca taagcgttat tttctgtgat ataggcgttt
     1561 ttgtaacttc cctcaaactc tatcgatttt accgtttgat aatcggtatc agacggcaac
     1621 ggaatccaca aattagttgt tcctttcgag ccttgtggca caactaaatc ataagattgc
     1681 gtaaattcgt aagtgtgggt agtggtgtgg aaattgttct gttgaacagg agcagcaaaa
     1741 gccgtcgcac agcttgaagc caccaaagag gcaataagtt tattcattga aatgatcctt
     1801 attagagtga aaaaacagtt aaaacttaac atattaagta ttctaactac ggattgaggt
     1861 tgcatattag ccaatcgaga tgggaaaagc aatgggggag atgatttttt agatagtgca
     1921 agcgtccacg cttgtactag aaacaaagct aaaataatca ataggcacaa gcgtggacgc
     1981 ttgcgccatc agagagaata acaagcggtc aaaaatcctc aaaattttac aaatagattt
     2041 tttgccctaa ttttgcactt tcaaatacgc tttcgatcac ttttagcaca aaaatgacgt
     2101 cttcgagttt gataattggc tcagcattgt gagcgatggc ttggtacaaa tcatcaaaca
     2161 agttttgata actgacttcg gcatttcgat agggctggcg aaccacatta ccttccactt
     2221 cagtgtgtaa aattccccag ttctcctttt cttcgatatt ccaatctttg ccaataggtt
     2281 gtacgccatt tttcagcaaa tcttcttgat tatcgggagt ttgtttcaga taagagccta
     2341 attttccgtg taatacaaat gtcgggcttg gttcacgagc atatcggctg gctttcagca
     2401 ccactttaaa accgttagca taatagagct gaatatcaaa gttatcatca accaatgccc
     2461 cttcgtgctg ataacgcaca tcagcataca ccgcctccgg cttcccgaaa agatacaaag
     2521 cttggtcaat taaatgtacg cctaaatcat acactaaacc tgtaccaagc tcgcctgatt
     2581 ctttccatac tttaacattt ttctctttcg cataacggtc aaaacgaatt tcgcaatcta
     2641 ccaactctcc cagtaaattt tgctccatta gcgattttgc cgtcgcaata tggctgtccc
     2701 aacggcggtt ttggtacaca taaagcacct ttccttgccg ctttgcaagt tccgccaact
     2761 cttccgcctc agccactgtt gccactaacg gtttttcaac taaaacgtgt ttgcctgcaa
     2821 gcaatgactc ttttaccatt gcataatggg tttgattcgg tgtggtaatc agcactaaat
     2881 ccacttctgg cgttaaaaga gcgttgaaat cccgcactgt ttctgcattc ggcaaccatt
     2941 ctgccgcatt gttagaggtt cgttcatata cctttacaat attaaaacga gcatcttgtt
     3001 tcaaaaacgg gatatgaaaa actcggctgg aaaagccggc acctgcgatg gcaatattga
     3061 tgggttgcat agacaatcct cataaaattt tgatgttatc tgcttaaata aacaagcggt
     3121 caaattttag gaaaattttg caaaaaacag ataaaatacg accgcttgtt taactaatca
     3181 taggttttgc aaacaatatc tttttcttca caaaacattt cactgtggtt tgcacggcat
     3241 tgcttttgta catttaattt ggctctgcct cggctactat gttcactacg atattcctga
     3301 gtaaatggct ttagagtaca aatgtgaacc tctttggaat caagtggatc tgagacacca
     3361 attataatcg gcatagggat accggcttgc cccgtaggag taaccggcac gacacagcca
     3421 cttaatagta tgcttaaact tattaaccaa taacgcatat ttttctcctt ttaaaatgta
     3481 taataaatca gaacggagca tctttatcgt tacagtaagc tcttaatatc ttgcctaatt
     3541 tcttggtatt tttattaaaa tttcggcatt tagggcatac caaaagatgc aactgcaaac
     3601 gcatatgttg ttgcaaacga agtttctcat cacaagactg tgaaataagt tctgttgctt
     3661 gcctgcattt cattttgatt cctctctaaa ccaattgcga gataaacact cttgtaaacg
     3721 taaacgagca cgatgaagta atacatttaa gttagagaga gaaatttcac attcttggca
     3781 aatttccgca ctactcatct ccaaatgttc acgcatcata aatactcgtg cttgctgggc
     3841 gggtaattta tttaaacaaa tatcaaaaat tgcccaaaat tctttttggt aagttgattg
     3901 agctaccccc ctccattcag ttacatcttg atgttccaac caatgatcgt ggcgatcgaa
     3961 aaaggcactt ggtgattctt cttcctcaca tagagaactg actggaatat gtcgttttcg
     4021 tgttctgagt aaatctaaaa ttttgttctt taaaatagca aaaatccaag ttttaagtgc
     4081 agcttctcgc cgaaaagagg ctgaatgctt ataggcattg gttaaggcat cctgtacaac
     4141 atcctcagct aaatctttat cggctagttg tagggtggca aatttaacca tttgctttct
     4201 aatctcttct atctgtgtag aagatagact
//
LOCUS       DANLYI010000189         4211 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.190, whole genome shotgun sequence.
ACCESSION   DANLYI010000189 DANLYI010000000
VERSION     DANLYI010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4211
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.190"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            58..981
                     /gene="oppB"
                     /locus_tag="PXD81_002147"
     CDS             58..981
                     /gene="oppB"
                     /locus_tag="PXD81_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HDL5538132.1"
                     /translation="MLKFIFKRLLEAIPTLLILITFSFFLMRLAPGSPFTSEKAYPPK
                     VMANIEAKYHLNEPLYKQYFLYMENLSKGDFGPSFKYKDQSVNNLIASAFPVSIKLGI
                     TAFIFAVLIGLTAGTIAALKQNSKWDYLVMGFSMTGIIMPSFVFAPLLVLMFAIYLKW
                     LPAGGWNGGQLYYMVLPVLSLTISYVAGIARITRGSMIEVLHSNFIRTAKAKGLPMRK
                     IIFKHALRPALLPVITYLGPAFVGIITGSMVIESVFGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGTLTILFNAIVDILYAVIDPKIRYS"
     gene            996..1895
                     /gene="oppC"
                     /locus_tag="PXD81_002148"
     CDS             996..1895
                     /gene="oppC"
                     /locus_tag="PXD81_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HDL5538133.1"
                     /translation="MQNSSKNQPLVEVTSTIQGRSLWQDARRRFFRNKAAVASLIILC
                     CIILFITFAPMLMPFSYEDTDWNMMGIAPDFESQHYFGTDSSGRDLLVRVAIGGRISL
                     MVGIAGALIAVLIGTIYGAISGYFGGKVDMVMMRFLEILSSFPFMFFVILLVTFFGQN
                     IFLIFIAIGMVAWLNLARIVRGQTLSLKNKEFVEAAIVCGVSRRQIIWRHIVPNVLGL
                     VVVYASLEVPSLILFESFLSFLGLGTQEPMSSWGALLSDGAAQMETSPWLLAFPAFFL
                     CLTLFCFNFIGDGLRDALDPKDK"
     gene            1905..3500
                     /locus_tag="PXD81_002149"
     CDS             1905..3500
                     /locus_tag="PXD81_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL5538134.1"
                     /translation="MELLAVNNLDVYLKTDEELVHAVRNVSFSIEKGQTLAIVGESGS
                     GKSVTSMAIMQLLPKNIVSFGERSSIIFEEQNLLTLSEKAMQTVRGDRIGMIFQEPMT
                     SLNPYMPIGKQVAETVQTHNPQISNAEAEKMVLETLQKVKIPDAEKKMICYPHEFSGG
                     QLQRIMIAMAIINKPDLLIADEPTTALDVTTQAEILDLMHDLQRDMGMAIILISHDLR
                     LVHKYSDLVCVMQNGEIIERGETETVFTNPQHPYTVELLTPIPTNLKGELPPNAPTLL
                     QGEHIKVDYILKRSLFGKPKKVFNALKDISLHLKTGETLGIVGESGSGKSTLGRAIMQ
                     ILDYRGNITFAGKTLANLNKAERQALKKDMQMVFQDPFNSLSPRLTVGEIIAEGLTVH
                     CPQMSKTERREKVMKMLEEVNLNPAMINRYPHEFSGGQRQRIAIARAIILEPKFVLLD
                     EPTSALDRSTQITVIELLNRLQKKYGLSYIFISHDLAVIKALSDRVIVMSQGEIVESG
                     DVTQIFENPQQEYTKRLILASNL"
     gene            3680..4075
                     /locus_tag="PXD81_002150"
     CDS             3680..4075
                     /locus_tag="PXD81_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HDL5538135.1"
                     /translation="MKKLMIFATTAMIVSNLAHAAGEQSDAREAHESTVKTSTVKYSC
                     QNGKKLSVKYGFNKQGIPTYAEAKLSGKKRFMPINLYTTDATGTNFGDENNFSLYGDP
                     MEFTNHRKASVNIQSPASEILYKGCTPQK"
BASE COUNT         1216 a          832 c          889 g         1274 t
ORIGIN      
        1 aagccgttaa tttacgctat taattgccta ccaatataac taaaaagagt ggtgcttatg
       61 ctgaagttta tttttaaacg tcttcttgag gcgattccaa ccctactaat tctgattacc
      121 ttttcctttt tcctgatgcg gcttgccccg ggtagcccat ttaccagtga gaaagcctat
      181 ccgccgaaag taatggcaaa tatcgaggca aaatatcacc tgaatgaacc gctgtataag
      241 cagtattttc tttatatgga aaatctgtca aaaggcgatt tcggtccatc ttttaaatat
      301 aaagatcaat cggtaaataa tctgattgct tctgcgtttc ctgtttcgat aaaactgggc
      361 attactgcct ttatttttgc ggtgcttatc ggtttaaccg caggaactat cgctgcactt
      421 aagcaaaata gtaaatggga ttatttagtg atgggctttt caatgacggg tattattatg
      481 ccgagctttg tctttgctcc gctgttggta ctcatgtttg cgatttatct caaatggttg
      541 cctgccggtg gttggaacgg tggtcagctc tactatatgg ttttacctgt tctctcttta
      601 accattagct atgttgccgg cattgcccgt attactcgtg gttcaatgat tgaggtgttg
      661 cactctaatt ttattcgcac cgctaaggca aaaggcttgc caatgcgtaa aattattttc
      721 aaacacgctt tgcgtccggc attgttgcct gtgatcactt atttaggccc ggcttttgtt
      781 ggcattatta ccggctcgat ggtaattgaa agcgtgtttg gcttaccggg aatcgggcag
      841 ttgttcgtaa acggagcatt aaatcgtgac tattcattag tattaagcct aaccattttg
      901 gtaggtacgc tcaccatttt atttaatgcg attgttgata ttttatatgc ggtcatcgac
      961 ccgaaaattc gttattcata ggagtaaagc ggaaaatgca aaattcttca aaaaatcaac
     1021 cgcttgttga ggttacctca accattcaag gacgaagttt atggcaagat gcacgccgcc
     1081 gttttttccg aaataaggcg gctgtggcaa gtttgattat tctttgttgt attattcttt
     1141 ttattacctt tgccccaatg ctaatgccgt ttagctatga agataccgat tggaatatga
     1201 tggggattgc tccggatttt gaatctcaac actatttcgg tactgattct tcagggcgtg
     1261 atttgctggt tcgagttgca atcggtggac gcatttcgtt aatggtaggg attgccggtg
     1321 cattaattgc ggtgcttatc ggtacaatct atggagcaat ttccggttat ttcggcggta
     1381 aagtcgatat ggtgatgatg cgttttctgg aaattttaag ctcattccca tttatgttct
     1441 tcgtaatttt gctggttacg ttcttcgggc agaatatttt cctgattttc attgcaatcg
     1501 gtatggtagc gtggctgaac cttgcccgta ttgtacgtgg tcaaactctt tcgcttaaaa
     1561 ataaagagtt tgtggaagca gcgattgtgt gtggcgtttc ccgccgtcaa attatttggc
     1621 gacacattgt gcctaacgtt ttaggcttag tggtggttta tgcctccctt gaagtaccat
     1681 ctctgatttt gtttgaatcg ttcttaagtt tcttagggct tggtacacaa gagccgatga
     1741 gcagttgggg ggcattatta agcgacggag cggcacaaat ggaaacctcg ccttggctac
     1801 tggctttccc tgcctttttc ttatgtttaa cactgttttg ttttaatttt attggtgatg
     1861 gcttgcgtga tgccctcgat ccaaaagata aatagaggtt tagaatggaa ttattagcag
     1921 taaataatct cgatgtttat ttaaaaacgg acgaagaatt agttcacgca gtgcgtaatg
     1981 tctcgttctc gattgaaaaa gggcaaaccc ttgcgattgt gggggaatcg ggttcgggta
     2041 aatccgttac ttcaatggca attatgcagt tattgcctaa aaatatcgtc tcattcggtg
     2101 aacgctcaag catcattttt gaagaacaaa acttgcttac tctatctgaa aaagcaatgc
     2161 aaacagttcg aggtgatcgc atcggtatga ttttccaaga gccaatgacc tcactcaatc
     2221 cttatatgcc gattggcaag caggttgctg agacggttca aacccataat ccgcaaatca
     2281 gcaatgccga agcggaaaaa atggtgttgg aaactttgca aaaagtaaaa attcccgatg
     2341 cggaaaagaa aatgatttgc tatccgcacg agttttccgg cggtcagcta caacgaatta
     2401 tgattgcaat ggcaattatc aacaaacccg atttattgat tgccgatgag ccgaccaccg
     2461 ccctggatgt cacaacccaa gcggaaattt tggatttaat gcacgactta cagcgtgata
     2521 tgggaatggc gattatctta atttcccacg atttacgctt ggtgcataaa tacagtgact
     2581 tagtctgcgt aatgcaaaac ggcgaaatta ttgagcgagg cgaaacggaa actgtgttca
     2641 ccaatccgca acatccttat acggtggagc tacttacccc gattccaact aatttaaaag
     2701 gggaattacc gccaaatgca ccaaccttgc ttcagggtga gcatattaag gtagattata
     2761 ttttaaaacg ctcgctattc ggtaagccga aaaaggtgtt taatgcgtta aaagatattt
     2821 cattgcactt gaaaaccggc gaaacgctcg gcattgtagg cgaatccggc tccgggaaat
     2881 ctacccttgg gcgtgccatt atgcagattt tagattatcg cggcaatatt acatttgcag
     2941 gtaagacact ggctaatctg aataaagccg aacgtcaagc cctgaaaaaa gatatgcaaa
     3001 tggtgttcca agacccgttc aactcacttt ctcctcgctt aacagttggg gaaattattg
     3061 ctgagggctt aaccgtacat tgccctcaaa tgagcaaaac cgaacggcgg gaaaaagtaa
     3121 tgaaaatgtt ggaagaggtt aatctcaacc ctgcaatgat taaccgctat ccgcacgaat
     3181 tttcgggcgg acaacgccag cggattgcga ttgcaagggc aatcatttta gagccgaaat
     3241 ttgtattgct tgatgagcca acctcagcac ttgaccgctc tactcagatc actgtaattg
     3301 aattgcttaa ccgcttacag aaaaaatacg gcttaagtta tattttcatc agccacgatt
     3361 tggcggtcat caaggcactt agcgaccgag tgattgtaat gagccaaggt gagattgtag
     3421 aaagcggtga tgtcactcaa atttttgaaa atccacaaca agaatacacc aagcggttga
     3481 ttttagcctc taatttgtaa ccccttccac aaggtcttat tctaccaaaa aacaagacct
     3541 tgtcatttta tgctagcaaa acattgtaat tttgatattt tatttcaatt ttttgaaaaa
     3601 atatggaact ttatataaaa ctcactttct aaacaatacg ttatctgaac taagataacg
     3661 cttatttaaa ggaaaatata tgaaaaaatt gatgatattc gcaacaactg ccatgattgt
     3721 aagcaattta gcccatgcag caggtgaaca aagtgatgca agagaagccc acgaaagcac
     3781 cgtaaaaacc tcaactgtta agtatagctg ccaaaacggc aaaaaattaa gcgtgaaata
     3841 cggcttcaat aaacaaggca ttccaaccta tgctgaggca aaattaagcg gtaaaaaacg
     3901 ttttatgccg atcaacctat acacgacaga tgcaaccggc actaactttg gtgacgaaaa
     3961 caatttcagc ctttacggtg atccaatgga atttaccaac caccgtaaag catcagtaaa
     4021 tatccaaagc cccgcaagtg aaattttata taaaggttgc acacctcaaa aatagataaa
     4081 aagtaaaatg agtccgctct gataagtggc tttttaatag ctgtttattg cagataggaa
     4141 agcattttgt ttttatctac tatccagaaa caagcggtta aatttgttta attttttaca
     4201 aaaaatgggc c
//
LOCUS       DANLYI010000190         4205 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.191, whole genome shotgun sequence.
ACCESSION   DANLYI010000190 DANLYI010000000
VERSION     DANLYI010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4205
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.191"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            25..1233
                     /locus_tag="PXD81_002151"
     CDS             25..1233
                     /locus_tag="PXD81_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011848442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HDL5538136.1"
                     /translation="MSKNLILILNCGSSSLKFAILDPKTGDEKLSGLAEAFHLEDARI
                     KWKLNGEKGNADLGAGAAHSEALTFIAEKLLSDELKAGIGAIGHRIVHGGEKFTSSVV
                     ITDEVIKGIEDATQFAPLHNPAHLIGIKEAFRIFPELKDKNVAVFDTAFHQTMPEHAF
                     LYALPYTFYKENGIRRYGFHGTSHFYITSQVAELAGKSVEQTNAIICHLGNGGSVSVV
                     RNGKCIDTSMGLTPLEGLVMGTRSGDLDPAIISFLYNNLGMSMDEIDTTLVKKSGLLG
                     LTGVTSDCRYAEDNYNDVSKPEAKRALDVYSYRLAKYIGSYMAILGDDHLDAIAFTGG
                     IGENSGLVRELTLNHLKLFGIKLDQERNLAARFGKDGVITADESTFKAVVIPTNEELV
                     IAQDTAKLAL"
     gene            1286..3421
                     /gene="pta"
                     /locus_tag="PXD81_002152"
     CDS             1286..3421
                     /gene="pta"
                     /locus_tag="PXD81_002152"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005542370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HDL5538137.1"
                     /translation="MSRTIILIPISTGVGLTSVSLGLVHALEQKGSKVGFLKPISQPI
                     SGEDTLDRSTTIIRAEQTTEVGEPFMLSEAETLIGQNQTDVLLEKVVERHQKLSKNNE
                     IVIVEGLIPTRKNSYANSINYEMAQALDAEIVLVAAPASDTPAQLKERVEAAASQFGG
                     RNNPNLLGVIINKFNAPVDDSGRTRPDLTEIFDSFQHSNNNVSEVEHLFAKSPIKLLG
                     CISWKAELIATRAIDVAKHLGASIINEGEIKARRIRGVTFCARNLPNMVDHFKAGSLL
                     VTSADRPEVLVAASLAVMNGIEIGAILLTGGYKIDSSIAKLCQQAFETGVPVFRIEGN
                     TWQTALSLQSFSLEVPVDDKERIAAIKEYMASKFNVGFIDEVSKAATRARRLSPAAFR
                     YQLTEYARQAKKRIVLPEGDEPRTVKAAALCAERGIAECVLLANPTDVQRVAEAQGVV
                     LGKGVTIIDPASIRENYVDRLVELRKAKGMTEVVAREQLLDTVVLGTMMLEANEVDGL
                     VSGAVHTTANTIRPPMQIIKTAPGSSIVSSIFFMLLPDQVLVYGDCAVNPDPTAEQLA
                     EIAIQSAESAKAFGIDPRVAMISYSTGTSGSGADVEKVKEATRIAQEKRPDLIIDGPL
                     QYDAAIMEDVARSKAPNSPVAGKATVFVFPDLNTGNTTYKAVQRSADLVSIGPMLQGM
                     RKPVNDLSRGALVDDIVYTIALTAIQATQ"
     gene            3535..>4205
                     /gene="can"
                     /locus_tag="PXD81_002153"
     CDS             3535..>4205
                     /gene="can"
                     /locus_tag="PXD81_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005597113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HDL5538138.1"
                     /translation="MEKIERLFANNHAWATKMKDEQSDYFKELAEHQKPTYLWIGCSD
                     SRVPAEKLTGLGPGELFVHRNVANMVIHTDLNCLSVVQYAVDVLEIEHIIICGHTNCG
                     GINAAIAQKELGLINNWLLHIRDIWFKHSHLLGKLPYEERSNVLTRLNVAEQVYNLGQ
                     SSIIKTAWEQGKSLSLHGWVYDVTDGFLIDQGVMATSRETLEITYRNAIAKITTEANE
                     KIVLN"
BASE COUNT         1313 a          783 c          863 g         1246 t
ORIGIN      
        1 ttcataaaat aaggtaattt gaccatgtct aaaaacttaa tcttaatcct taactgcggt
       61 agttcatctc ttaaatttgc tattttagat cctaaaacag gtgatgaaaa attatctgga
      121 cttgctgaag cttttcattt agaagatgca cgtatcaagt ggaagttaaa cggggagaaa
      181 ggtaatgctg atttaggtgc aggtgccgca catagtgaag cacttacctt tattgctgaa
      241 aaattgttat ctgacgaatt aaaagcaggt atcggcgcga ttggtcatcg tattgttcat
      301 ggcggtgaaa aattcacctc atcagttgta attaccgatg aagtgattaa aggtattgaa
      361 gatgcaacgc aatttgcacc attacacaac cctgcgcatt taattggtat taaagaagca
      421 ttccgcattt tccctgaatt aaaagacaaa aacgtagctg tatttgacac tgcattccat
      481 cagacaatgc cggaacacgc attcttatat gcacttccat atacattcta taaagaaaat
      541 ggtattcgtc gctacggttt ccatggcaca agccacttct atatcacctc acaagttgca
      601 gaattagcag gtaagtctgt tgagcaaacc aatgccatta tttgccactt aggtaacggt
      661 ggttctgttt ctgttgtacg caatggtaaa tgtattgata cctcaatggg tttaactcca
      721 ttagaaggtt tagtaatggg tactcgctct ggtgacttag accctgcgat tatctctttc
      781 ttatacaata acttaggtat gtcaatggac gaaattgata ctactctagt aaaaaaatct
      841 ggtcttttag gtttaactgg cgtaactagt gactgtcgtt atgcggaaga taattacaat
      901 gacgtatcaa aaccggaagc aaaacgtgct ttagatgtat atagctatcg tttagctaaa
      961 tatattggtt catatatggc aattttaggc gatgaccact tagatgctat cgcatttaca
     1021 ggtggtatcg gtgaaaattc aggcttagta cgtgaattaa ccttaaatca cttaaaatta
     1081 tttggcatta agttagatca agagcgtaac cttgctgccc gttttggtaa agatggagtc
     1141 attacagcgg acgagtcaac atttaaagct gttgttatcc caactaatga agaactcgtg
     1201 attgcacaag ataccgctaa attagcatta taatcatagg gggcagatat tctgcccttt
     1261 cactttcttt taatttggaa atattatgtc tagaacgatc attcttatcc ctatttcaac
     1321 tggtgttggt ttaaccagtg ttagcttagg tttagtacat gctttggagc aaaaaggttc
     1381 taaagtagga tttttaaaac cgatttctca acctatcagc ggtgaagata cgttagaccg
     1441 ctcaacaacg attatcagag cagaacaaac aacagaggtt ggtgagccgt ttatgttaag
     1501 cgaagctgaa acattaatcg gtcaaaacca aacggatgta ttgttagaaa aagtggtgga
     1561 acgccaccaa aaattatcaa aaaataatga aattgtcatt gttgaaggtt taattccaac
     1621 gcgtaaaaac tcatacgcta acagcattaa ctatgaaatg gctcaagccc ttgatgctga
     1681 aattgtatta gttgcagcac cggcctcaga tacaccagct caattaaaag agcgagttga
     1741 agctgccgct tcacaatttg gtggacgtaa caacccaaat ttactgggcg ttattatcaa
     1801 taaattcaat gcaccagtag atgattcagg tagaacccgt ccagatttga ctgaaatttt
     1861 tgattcattt caacactcaa ataataatgt ttcagaagtc gagcatcttt ttgctaaaag
     1921 tccaattaag ctgttaggct gtattagctg gaaagcagaa cttattgcta cacgagcaat
     1981 tgatgttgca aaacatttag gcgcttcaat tattaatgaa ggcgaaataa aggctcgtcg
     2041 gattcgcggt gtgacattct gtgcacgtaa cttaccaaat atggtcgatc attttaaagc
     2101 cggtagttta ttagttacct ctgcggatcg tcctgaagtg ttagttgctg catcgttggc
     2161 ggtaatgaat ggcatcgaaa ttggtgctat tttactaacc ggtggttata aaattgatag
     2221 ctcaattgca aaactttgcc aacaagcatt tgaaaccggt gtgcctgtat tccgtattga
     2281 aggtaacact tggcaaaccg cattaagcct acaaagcttc agcttagaag tgcctgtaga
     2341 tgataaagag cgtattgctg caattaaaga atatatggca agcaaattta atgtaggttt
     2401 cattgatgag gtttctaaag ccgctactcg tgcaagacgt ttatcacctg cagcattccg
     2461 ttaccaatta accgaatatg cccgtcaagc gaaaaaacgc attgttttac ctgaaggaga
     2521 tgagcctcgt actgtgaaag cggctgcatt atgtgctgag cgtggcattg cagaatgtgt
     2581 gttattagct aacccaacag acgtacaacg tgttgctgaa gcacaaggcg tggtattagg
     2641 taaaggcgta acgattattg accctgcgag cattcgtgaa aattatgttg atcgcttagt
     2701 tgaattgcgt aaagcaaaag gaatgactga agttgttgct cgtgagcaac ttttagatac
     2761 agttgtattg ggtacaatga tgcttgaagc taatgaagtg gacggtttag tctcaggcgc
     2821 agttcacaca accgctaaca ctattcgccc accaatgcag atcattaaaa ctgcaccggg
     2881 aagctctatt gtatcttcta tcttctttat gctactacct gatcaagtat tggtatatgg
     2941 tgactgtgcg gtaaaccctg acccaactgc agaacagcta gcggaaattg cgattcaatc
     3001 agcagaatct gccaaagcct ttggtattga ccctcgtgtt gcaatgatct cctactctac
     3061 cggtacatca ggttcaggtg cggatgtaga aaaagtgaaa gaagcaactc gtattgccca
     3121 agaaaaacgt ccagatttaa tcattgatgg cccattacaa tatgatgcgg cgattatgga
     3181 agatgttgcc cgctcaaaag caccaaattc acctgtagca ggtaaagcaa ctgtatttgt
     3241 cttcccggat ttaaatactg gtaataccac ctataaagcg gtacaacgtt cggcagattt
     3301 agtctctatc ggcccaatgt tacaaggtat gcgtaaacct gtaaatgact tgtcccgtgg
     3361 ggctttagtt gacgacatcg tttatactat tgcgttaact gcgattcaag caacgcaata
     3421 attccctccc aaatgaaacc aaatgccctt aaaactaagg gcatttttat tttttaccta
     3481 cccgactttt caatttaatc atataatcaa gattagtgtt aaaaaggaga atttatggaa
     3541 aaaattgaac gcttatttgc caataaccac gcctgggcaa ctaagatgaa agatgaacaa
     3601 tcggattatt ttaaagaatt agcggaacat caaaagccaa cctatttatg gattggctgt
     3661 tccgatagtc gtgtgcctgc tgaaaaactc acagggttag gccccggaga attatttgtt
     3721 catcgaaatg tagcaaatat ggtgattcac actgatctta attgtctgtc ggttgttcaa
     3781 tatgcggtag atgtattaga aattgagcat attattatct gtggtcacac taattgtggt
     3841 ggtatcaatg cggcaattgc acaaaaagaa cttggtttaa ttaataactg gttgttacat
     3901 attcgtgata tctggtttaa acacagccat ttactaggca aacttcccta tgaagagcga
     3961 tcaaatgtct tgacccgatt aaatgttgca gaacaagttt ataatttagg tcagtcctct
     4021 attattaaaa ccgcttggga acagggtaaa tccctctcgc tacacggttg ggtatacgat
     4081 gttacagatg gtttcttgat tgaccaaggt gttatggcta ctagccgaga aacattagaa
     4141 attacctatc gtaatgcaat agctaaaata acaaccgaag caaacgaaaa aatagtttta
     4201 aacga
//
LOCUS       DANLYI010000191         4140 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.193, whole genome shotgun sequence.
ACCESSION   DANLYI010000191 DANLYI010000000
VERSION     DANLYI010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4140
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.193"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            331..1533
                     /gene="yedE"
                     /locus_tag="PXD81_002154"
                     /pseudo
     CDS             331..1533
                     /gene="yedE"
                     /locus_tag="PXD81_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005824242.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
     gene            1569..1838
                     /gene="yedF"
                     /locus_tag="PXD81_002155"
     CDS             1569..1838
                     /gene="yedF"
                     /locus_tag="PXD81_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007241376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HDL5538139.1"
                     /translation="MAFTVVQKLDKDPKDITPDYTLDTLGEPCPYPAITMLETMPQLQ
                     KGEILELLSDCSQSINNIPIDTKNHGYTLLSVEQNGTKLRYLIQR"
     gene            1938..2282
                     /locus_tag="PXD81_002156"
     CDS             1938..2282
                     /locus_tag="PXD81_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HDL5538140.1"
                     /translation="MNKYALLAIFWFIIGIYALLFRESTNDIPPFPHFDKVSHFALFF
                     IQIWLSANAFIRSKKGIPYFGLIIFALLYAFTSEWAQATFTSTREGSWLDGLADMLGA
                     SLSLLLVKYCKK"
     gene            complement(2345..3292)
                     /locus_tag="PXD81_002157"
     CDS             complement(2345..3292)
                     /locus_tag="PXD81_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HDL5538141.1"
                     /translation="MNRITQILNIKHPIVQAPMSWLTDAHLVASVAEAGGLGFLAPHA
                     GQTTNPTSNEEVLDRMRNEIRKVKALTDKPFGVPFILSYDFSLIPLMVDLFIEERVPV
                     VLDNGWLDPEIYAKFKAAGIKIICRLPNPNLADALKAQALGADILVLTGFDEGGTLPM
                     KEVGSFNVLAEFVGKVDLPIMLAGGIADKKAVQAALTLGAEGVWIGTAFIATEECRAS
                     EKVKNWIVEANANDLLLFRTEPHYYRSLPTGLAKKCFEMSENGATRSEIAKVMNAGTG
                     MRLGMLEGDFDNGYVSVGNGISQINKIKSVKELIDELMA"
     gene            3475..>4140
                     /locus_tag="PXD81_002158"
     CDS             3475..>4140
                     /locus_tag="PXD81_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5538142.1"
                     /translation="MSIRWENIKPLQGSQNSAFEELVCQLARQEFKSQGKFTRISAPD
                     GGIEAMCELPDGSVCGWQAKYFLSSFSSSQWQQIEDSFKESLKNYPKLTKYYVCVATD
                     RANANVSGKKSFLTKWEEYTQRWKDFAQSQGREIEFEFWGSFELSDLLSKPENAGKKF
                     FWFNESELSDKWFEQYNQQAINNLGVRYTPEINVDLPIAMQLESLARTQIFKKHLSDE
                     FHKL"
BASE COUNT         1219 a          852 c          813 g         1256 t
ORIGIN      
        1 aaccggataa aacaatgagt gaaggttatg aacgctttat gcaacgttat aagcaaggca
       61 ttcctgtggt agaaaaagcg ttgtttttaa atacagatta aataattata atttaagtat
      121 cacaagcggt tatttttgca aaaaacgacc gcttgttatt tacatttgta caaaaaatac
      181 ctactataaa gacgcatttg taaaatgtgc attgatatat cgcaagtttt gtgtatagga
      241 aatgaactaa ttactgtgtc aatcagtccc tttcttcatc gttataccct cttgcaaatc
      301 cgattaatct atccttagtt aaggtgtttt atgttagcga tttggcaaag ttttaaacaa
      361 aattatctaa tcaaatattg gaatcctgtt gcagcagtga tcgcagctgg tattatttca
      421 gcttattatt tcggcattac tggcacttat tgggcggtta caggggaatt tacccgttgg
      481 ggcggtcatc tgttaaatgc tttgggtgtt gatgtttcga actggagcta ttttcaactc
      541 atcggcttag acggcaccat ctttacccgc attgacggtg tgatgattct cggtatgttt
      601 gccggctgtc tttctgccgc attatgggcg aacaacgtca aatggcataa tcagccacat
      661 aaaaaacgga ttatccaagc cttgattggc ggtgcgttag ccggtttcgg agcccgttta
      721 gcaatgggct gtaacctcgc ctctcttttt accggtattc cgcaattctc cgtccacgct
      781 tggtttttca cctttgcgac cgccatcggc acttactttg gggtaaaagt gactttatta
      841 ccgatgttta gagtgaaatt agagctgaaa aaaggggcag caaaaatcaa agaaaacgat
      901 ccgaaacaag ctaatcgccg tttttgggtt ggtatgctca tttttatcac ttatctttct
      961 gcttcccttt atgtaatgca atcctcaatc aaacttggtt ttgcaatgct ttgcggctta
     1021 gcctttggct tattgattga acgagcccaa atttgcttta cctccgcttt ccgtgactta
     1081 tggattaccg gacgggctta tatggcaaaa gcaattattt tcggcattat tgccggcaca
     1141 atcggcgtgt tctcttatat tcaattaggt gtttcaccta aaattttgtg ggctggccca
     1201 aatgccatta tcggcggatt attattcgga tttggcattg tgcttgccgg cggctgtgaa
     1261 accggctgga tgtaccgttc aatggaagga caggttcatt ttatgtgggt cggcgtaggc
     1321 aatgtagtcg gttcaaccct gctggcttac tactgaggcg acttagctcc ggtgcttacc
     1381 cttgattatg aaaaaatcaa cctactcaaa gaatttgggc cggttggcgg attattcatc
     1441 aattatggct tattaattct ttgcttaatc gctgtagttt ggtgggaaaa acgcttcctt
     1501 gccaaagcga aagcaaaatt agcaactgcg taatcttttt aacttcaacc aacaagagga
     1561 aaatgactat ggcattcacc gttgtacaaa aactagataa agatccaaaa gacatcacgc
     1621 ctgattacac cttagatacc ttaggcgaac cttgccctta ccctgcgatt acgatgttag
     1681 aaacaatgcc acagctgcaa aaaggagaga tattggaact gttaagcgat tgttcccaat
     1741 cgattaataa tattccgatt gatactaaaa atcacggcta taccctatta agcgtggaac
     1801 aaaacggcac taaattacgc tatttaattc agcgttaatt gcaaaaaatt cgcacaaaac
     1861 gaccgcttgt cgtaaaatag caactattta agagccttgt aacgcaaggc tcttttattc
     1921 taagaggtaa aagaaaaatg aataaatacg ctttacttgc aatattttgg tttattattg
     1981 gcatttatgc tttacttttt cgtgaaagca ctaatgatat accgcctttt ccacattttg
     2041 ataaagtaag ccattttgcc ttattcttta tccagatctg gttatctgca aacgccttta
     2101 tccgttcaaa gaaagggata ccttattttg ggctaataat ttttgcctta ctctatgctt
     2161 tcaccagcga atgggctcag gcaaccttca cctcaacccg agaaggttct tggcttgatg
     2221 ggctagcaga tatgctagga gcctcacttt cattattgct ggtaaaatat tgcaaaaaat
     2281 aaagcataaa agaccgcttg ttatatggtt tagccatact acaagcggtc attttttacc
     2341 aaaattatgc catcaactca tcaatcaatt ctttcacact tttgattttg ttgatttgtg
     2401 aaatgccatt gcccaccgac acatagccat tgtcaaaatc cccttctaac atacccaaac
     2461 gcattcctgt accagcgttc attaccttag caatctcact acgagttgcc ccattttcgc
     2521 tcatttcaaa gcattttttc gctaatccag tcggcagtga acggtagtaa tgtggttcgg
     2581 tacggaataa cagcagatca ttggcatttg cctccacaat ccagtttttc actttttcgc
     2641 ttgcacggca ttcttcggtg gcgataaatg ccgtgccaat ccacacgcct tcagccccaa
     2701 gcgttaaggc agcctgcacc gcttttttat cggcaatacc accagccagc attatcggca
     2761 aatccacctt gcccacaaat tccgccagca cattaaaact gcctacctct ttcatcggca
     2821 aagtaccgcc ctcatcaaaa cccgtcagca ccaaaatatc tgctcccaat gcttgggctt
     2881 ttaaagcgtc cgccaaattg ggattaggca aacggcagat gattttaatg ccagcagctt
     2941 tgaatttggc gtaaatctct gggtcaagcc agccgttatc cagcaccaca ggcacacgct
     3001 cttcaatgaa taaatccacc attaacggaa tcagcgaaaa atcataagac aaaataaatg
     3061 gtacgccaaa aggcttatcg gtcaatgcct taaccttacg aatctcgtta cgcatacggt
     3121 caagtacttc ttcgttggaa gttggattgg tggtttgccc tgcgtgtggg gctaaaaaac
     3181 caagcccacc agcctcggca acgcttgcta ccagatgagc gtccgtcagc caagacattg
     3241 gcgcttggac gatagggtgc ttgatattta agatttgggt aatgcggttc attggatttt
     3301 cctgtttgaa taaaaagaat tggacgtagt ttaatggttt tggatttgag agggaatgcg
     3361 tgaaaatggg aaggtggttt tcggatttgg aaaattatca atgctaaaat acttgaaata
     3421 cacacgccaa tcctcatatc aacaaaattg aatatttaat tatggtaaaa gaaaatgtct
     3481 attagatggg aaaacatcaa gcccttacaa ggttctcaaa atagtgcttt tgaagagcta
     3541 gtatgccaat tggcacgcca agaatttaaa tctcaaggaa aatttactcg aatttctgca
     3601 cctgatggtg ggattgaagc aatgtgtgaa ttgccagatg gttccgtatg tggttggcaa
     3661 gccaaatatt ttttaagctc attttcttca tcacaatggc aacaaattga agattccttt
     3721 aaagaatctt taaaaaacta tcctaaacta accaaatact atgtttgtgt ggcaaccgat
     3781 agagcaaatg ccaatgtatc tggaaagaaa tcatttctta ccaaatggga ggagtatacc
     3841 caaagatgga aagactttgc tcaatcacaa ggtcgtgaaa tagaatttga attttgggga
     3901 agttttgaat tatctgactt attatcaaaa cctgaaaatg caggcaaaaa attcttttgg
     3961 tttaacgaaa gcgaattatc tgacaaatgg tttgaacaat acaatcaaca agccattaat
     4021 aatcttggtg tgcgttatac acctgaaatt aatgttgatt tacctattgc aatgcaatta
     4081 gaaagcttgg caagaaccca aattttcaaa aaacatctga gtgatgaatt tcataaattg
//
LOCUS       DANLYI010000192         4130 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.194, whole genome shotgun sequence.
ACCESSION   DANLYI010000192 DANLYI010000000
VERSION     DANLYI010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4130
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.194"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            complement(112..861)
                     /gene="moeB"
                     /locus_tag="PXD81_002159"
     CDS             complement(112..861)
                     /gene="moeB"
                     /locus_tag="PXD81_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HDL5538143.1"
                     /translation="MELTDHEMLRYNRQIILKSVDFDGQEKLKASRVLIVGLGGLGCA
                     ASQYLASAGVGHLTLVDFDLVSLSNLQRQILHTDATIGQPKVESAKARLAAINPNIQI
                     ETINQRCSDEQLAKLIANVDLVLDCTDNVTVRNQLNLQCFKQKRPLVSGSAIRFEGQV
                     SVFCYQEQEPCYECLSRLFGDATLSCVEAGVIAPIVGIIGSIQALEAIKVLLNIGRTL
                     SGKLLMIDGLHFSVREMALPKQNNCPVCTSY"
     gene            complement(960..2189)
                     /gene="moeA"
                     /locus_tag="PXD81_002160"
     CDS             complement(960..2189)
                     /gene="moeA"
                     /locus_tag="PXD81_002160"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HDL5538144.1"
                     /translation="MALLALSDALDNMLARLSTPKLVEQLPLNECANRVLAEDIFSPI
                     NVPNFDNSAMDGYAVRISDLAQNLTLNVAGKAFAGNPFNGEIKAGECVRIMTGALVPK
                     GVDAVVMQEETTLNSDGSVTFNHIPKLGSNIRRIGEDVKQGALVLKKGSLLNVASLPL
                     LASLGIASVPVFVKLKVAILSTGDELTSVGEPLSEGKIYDTNRFAVRLMLEKLNCEIL
                     DFGILPDDPILFEQTFRQAQQQADVLITSGGVSVGEADFTKDVLEKLGEIGFWKIAMK
                     PGKPFAFGKLEQAWFFGLPGNPVSALVTFYQLAQPALAKLSGLSTDKIAKLTQNLTAC
                     SAGKLKKAVGRQDFQRGYYYANEKGELEVRAVGTQGSHIFSAFNESNCFIVLEAERGN
                     VDAGEKVTIQPFNSLMY"
     gene            complement(2211..2828)
                     /locus_tag="PXD81_002161"
     CDS             complement(2211..2828)
                     /locus_tag="PXD81_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006719828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HDL5538145.1"
                     /translation="MFDSLAVQFVVLWAVIDPIGSVPVYLAKTIGLPIEARRKIALNA
                     VLIAAGILIFFLIGGQVLLESMQIPLPAFQAAGGLVLLLFALTMIFGESKPEQEMRIS
                     SNLNELAVYPLAVPSIASPGAMMAIVLLTDNHRFHIGDQIITAGIMLLVLFITYLLLL
                     AANRIQHLIGNAGAAIISRVMGLILAAIAVNNLLIGVRDFFVQIS"
     gene            complement(2896..4107)
                     /locus_tag="PXD81_002162"
     CDS             complement(2896..4107)
                     /locus_tag="PXD81_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDL5538146.1"
                     /translation="MISRQMQSLLKRLVAQFPAVLVTGARQVGKSTLLQHIGEGYQYI
                     TFDDPMLLELAKQESRLFFLNHQGKLILDEVQYVPEIFALLKLEIDKKQQNGLFLLSG
                     SQAFELMQNVSETLAGRIAILKLQGLSLREILGVKFDQPFLPTTDYLLAREQELKSPE
                     NIWQLIHKGDMPRLYAQEVDWEIYYRSYVATYIERDVRQLTNIASTTDFTRFMVSIAS
                     RSGELLNYSNIAQEVGVSNETIKRWVAILQTSGIIYLLQPYHNNHLKRAIKTPKVYFL
                     DTGLMAYLTKWLTPETIAQGAKSWQFFETFVVSQIIKSFHNQGIEPPIYFYRDTNQKE
                     IDLIIEYDRTLYPIEIKVTASPTKKMAKSFDLLNSVSSEWKLGQGAIINQYPTKLYLS
                     ENLVALPVSYL"
BASE COUNT         1248 a          965 c          760 g         1157 t
ORIGIN      
        1 acggtgtaaa taataggcta aaacgtttta gccgtcaaat aaaaaaaata aactgtcgtt
       61 cattttttac atttcttgac attttttcgc caataagtat gagaaaaata cttaataaga
      121 ggtacaaacc ggacagttat tttgcttagg taatgccatt tctctgacag aaaaatgcaa
      181 gccgtcaatc attaacaatt ttccggaaag agttctgccg atattaagta gcactttaat
      241 tgcttccaac gcttgaatac tgcctatgat gccaacaatc ggagcaatga cccccgcttc
      301 aacacaactt aaagtcgcat caccaaacaa cctgcttaaa cattcatagc acggttcttg
      361 ttcttgataa caaaatacac tcacctgccc ttcaaatcga attgccgaac cggaaacaag
      421 cggtcgtttt tgcttaaaac attgcaaatt taactgatta cgcacagtaa cgttatcggt
      481 acaatccagc actaaatcta catttgcaat cagcttggct aactgctcat cggaacatcg
      541 ctgattgata gtttcaatct gaatgttcgg attaatcgcc gctaatcgag ctttggcaga
      601 ttccaccttt ggctgcccga tagtcgcatc ggtatgtaaa atttggcgtt gcagattaga
      661 aagtgagacc agatcaaaat ccaccaaagt caaatgccca acgccggcag aagctaagta
      721 ttgcgatgcc gcacagccca aaccacctaa accgacaatc aacactcggc tggcttttaa
      781 cttttcctgc ccgtcaaaat ccacgctttt taggataatc tggcgattat aacgtagcat
      841 ttcgtgatcg gtaagttcca tttttacacc taaagagtaa acgatggcgc aagcgtccac
      901 gcttgtgcct atatttttat tgctagcaca agcgtggacg cttgcgctat cttaattcat
      961 taatacatca gcgaattaaa cggctgaata gtgacttttt ccccagcatc aacattgcct
     1021 cgttctgctt ctagcacaat aaaacagtta ctttcattaa aagcgctgaa aatatgtgag
     1081 ccttgcgtgc caactgcacg aacttctaac tcaccttttt cattggcata gtaatagccc
     1141 cgttggaaat cttggcgacc gaccgctttt ttcaattttc cggcactgca agcggttaga
     1201 ttttgggtta attttgcaat tttatctgtc gataaacccg aaagtttggc taacgccggt
     1261 tgcgctaatt ggtagaaagt cactaatgca gagacagggt tgcccggcag cccaaaaaac
     1321 caagcctgtt ctaatttacc gaatgcaaaa ggcttgcccg gcttcatcgc aattttccag
     1381 aaaccgattt cgcctaattt ttccagtaca tctttggtaa aatctgcctc tcccacagat
     1441 acgccgccac tggtaatcaa cacatcggct tgctgttggg cttgcctgaa agtttgctca
     1501 aacagaattg ggtcatcagg caaaatccca aaatctaaaa tctcgcagtt gagtttttcc
     1561 agcattaaac gcaccgcaaa acggttggtg tcgtaaattt ttccctcact caaaggctcg
     1621 ccaactgagg ttaattcatc gccggttgaa agaattgcca cttttaattt tacaaaaaca
     1681 ggcacagaag caataccgag tgaagctagc aacggtaaac tggctacatt caataatgag
     1741 ccttttttca agaccaatgc cccctgttta acatcttccc caatgcggcg aatgttactg
     1801 cctaattttg gaatatggtt aaaggttaca gagccatcgg aatttaacgt agtttcctct
     1861 tgcattacaa cggcatcaac gccctttggc actaatgctc ccgtcataat ccgcacgcat
     1921 tcgcctgcct ttatttcgcc gttaaacggg ttaccggcaa aggctttacc agccacattc
     1981 aaggttaaat tttgtgctaa atctgaaata cgaaccgcgt agccgtccat cgccgaatta
     2041 tcaaaattcg gtacattaat cggggaaaaa atatcctctg ccaacactcg gttagcacac
     2101 tcatttaacg gaagttgttc caccaattta ggtgtcgata aacgagcaag catattatct
     2161 aacgcatcac tcaaagccaa aagtgccata atttcctacc tattcaacgt ttagctaatt
     2221 tgtacaaaga aatcccgtac gccaatcaac agattattca ccgcaatcgc agccagaatt
     2281 aaccccatca ctcggctgat aatcgcagcc cctgcattac caataaggtg ctgaatacgg
     2341 tttgccgcta atagcaacaa ataggtaata aagagtacca aaagcataat gccggcggtg
     2401 ataatttgat cgccgatatg aaaacggtga ttgtcggtta ataatacgat tgccatcatc
     2461 gccccgggag aagcaatcga cggtacagct agcggataaa ccgccaattc attcagatta
     2521 ctgctgatcc gcatttcttg ctcgggcttg ctttcgccaa aaatcatcgt taaagcaaat
     2581 aacaacagca ctaagccgcc tgccgcttgg aatgcaggta acggaatctg cattgattct
     2641 aacagcactt gcccaccgat aaggaagaaa atcaaaatac cggcggcaat caagaccgca
     2701 tttagtgcaa ttttacgccg tgcttcaatc ggcaaaccaa tggtttttgc caaataaaca
     2761 ggaacagaac caatcgggtc gatcaccgcc cataaaacca caaattgtac cgctaatgaa
     2821 tcaaacatat tctctcctgt tatcttgata atttgcaaaa ttttagcata aaacaaccgc
     2881 ttgcacggct ttttcttaca gatagctcac cggtaacgct accaaatttt cacttaaata
     2941 aagtttggtc ggatattggt taataatcgc tccctgccct aatttccatt cactagaaac
     3001 tgagtttagc aagtcaaaac tttttgccat ttttttggtt gggcttgccg tgaccttaat
     3061 ttctatcgga taaagcgtgc gatcatattc aataattaag tcgatctctt tctggttagt
     3121 atcacggtaa aaataaatcg gcggctcaat tccttgatta tgaaaggatt ttataatttg
     3181 gcttaccaca aaggtttcaa agaactgcca gctttttgcc ccttgagcaa tagtttcggg
     3241 ggtaagccat ttggttaaat acgccattaa cccggtgtct agaaaataca cttttggggt
     3301 cttaattgcc cgtttcaaat gattgttgtg gtatggctgg agcaaataga taatgccgga
     3361 agtctgcaaa atggcgaccc aacgtttaat cgtttcattc gatactccaa cttcttgagc
     3421 aatattactg taattgagta gctcaccact acgagaggca atagagacca taaaacgggt
     3481 aaaatccgtt gtgctggcaa tattggttaa ttggcgaaca tctcgctcaa tgtaggtcgc
     3541 tacataagag cgataataaa tctcccaatc cacctcttgt gcatataaac gaggcatatc
     3601 gcctttatgg ataagctgcc agatgttctc cggtgatttt aattcttgct cccgagctaa
     3661 caaatagtcg gttgtcggta aaaaaggttg atcgaacttc acccctaaaa tttcacgcaa
     3721 cgaaagccct tgtaatttta aaatagcaat acgaccggca agtgtttcac tcacattctg
     3781 cattaattca aaggcttgcg accccgaaag taaaaacaaa ccgttttgct gttttttatc
     3841 aatttctaac tttaataacg caaaaatttc aggcacatac tgtacttcgt ctaaaataag
     3901 cttgccttga tggtttaaaa aaaacaggcg agattcttgt ttagccagct ccagcaacat
     3961 tgggtcatca aaagtaatat attgatagcc ctcaccaata tgttgtaaca atgtcgattt
     4021 accaacctgt cttgcccctg tcactaaaac ggcaggaaat tgcgccacga gtcgttttaa
     4081 cagcgattgc atttgccgag aaatcatttt tcctccaaaa tagcctaaat
//
LOCUS       DANLYI010000193         4111 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.195, whole genome shotgun sequence.
ACCESSION   DANLYI010000193 DANLYI010000000
VERSION     DANLYI010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4111)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4111)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4111
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.195"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            7..462
                     /locus_tag="PXD81_002163"
     CDS             7..462
                     /locus_tag="PXD81_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015939902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HDL5538147.1"
                     /translation="MGCSSNSGESTSAVETTEANATWQSADSIQTAPMPASMNQPIAT
                     QPTYQQPTYSAPQPISAAPAPMPVSSGSDSSQVETVGNCQVVRDANNAPIYSQITKGC
                     YTESHYTVGKSDTLYLIGYLTGTNANQIAQLNGLNTGSPLKVGQVLRVR"
     gene            475..675
                     /locus_tag="PXD81_002164"
     CDS             475..675
                     /locus_tag="PXD81_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5538148.1"
                     /translation="MNRTLLAFAISCAFLTACSSPTQPQVEDPNMLPAGTLQPIEGSG
                     ASQDSYSWPSDIQSTSMPDSMK"
     gene            694..1974
                     /locus_tag="PXD81_002165"
     CDS             694..1974
                     /locus_tag="PXD81_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009874873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HDL5538149.1"
                     /translation="MKKQFILLPLVSAVLAACSSNNPAPVVNASGNNELSPGVMQPVD
                     VSAGAINSSGGWQSDIQSAPMPSSMNTTPVQTMPQPVSVPQPVATVPQPITNPVITEQ
                     PPQPTTTTKIVKKTKTVEKKVDQNFEIPRDANNAPMYNQIQKGFYDGSTYTVRKGDTM
                     FLIAYIVGKDVKEIAALNNMSEPYQLSVGQKLKTGKSATETITVEEKVTVPVEPQITY
                     QQGANGTTYASGGNITGPVKAGTANADVTPVNNGIKATTATAPAVTASVGTVNATEDR
                     TASTIRAIDGTASGAVQSSNTVSAPASSIKWQWPTSGRVISGFSSAEGGNKGIDIAGN
                     KGQDVRAAAAGKVVYAGNALQGYGNLIIIKHTDDFLSAYAHNNTIEVDEQETVKAGQK
                     IATLGSTGTNTNKLHFEIRYKGKSVDPARYLPRK"
     gene            2215..2406
                     /locus_tag="PXD81_002166"
     CDS             2215..2406
                     /locus_tag="PXD81_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5538150.1"
                     /translation="MDPTEPSHRIYILLSEDRFQAEVFSTKLSESSMLLEAVKGGYIS
                     KDGKVRLLKKAEGWKIYYE"
     gene            2399..2965
                     /locus_tag="PXD81_002167"
     CDS             2399..2965
                     /locus_tag="PXD81_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HDL5538151.1"
                     /translation="MSETKIRCDWCKSSEIYMKYHDEEWGKPEFDSLKLFEKICLEGQ
                     QAGLSWITVLKKREAYRQAFHQFNPEKIAKMGEEEIDLLMQNTSLIHHRAKLEAIIKN
                     AKAYITMQQNGEDFSRFIWSFVNYQPQINDVPTLEAVPAHTETSKAMSKALKKRGFVF
                     VGETTCYAFMQSMGLVNDHLNNCVFKHK"
     gene            complement(3012..3242)
                     /gene="acpP"
                     /locus_tag="PXD81_002168"
     CDS             complement(3012..3242)
                     /gene="acpP"
                     /locus_tag="PXD81_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005725021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HDL5538152.1"
                     /translation="MSIEERVKKIIVDQLGAKAEDVKPEASFIEDLGADSLDTVELVM
                     ALEEEFDIEIPDEEAEKITTVQSAIDYVQNNQ"
     gene            complement(3429..4106)
                     /gene="rpe"
                     /locus_tag="PXD81_002169"
     CDS             complement(3429..4106)
                     /gene="rpe"
                     /locus_tag="PXD81_002169"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HDL5538153.1"
                     /translation="MSKNPYLIAPSILSADLARLGDDVAEVLKAGADLIHFDVMDNHY
                     VPNLTFGPAICKALRDYGIQCPIDVHLMVKPVERLIPDFAKAGADYITFHPEATEHID
                     RNLQLIRDLGCKSGLVFNPATPLSYLDYVMDKVDVVLLMSVNPGFGGQSFIPSTLDKL
                     KEVRRRIDESGYNIRLEVDGGVKINNIADIAAAGADMFVAGSAIFDQPDYQAVIAQMR
                     DELRKVG"
BASE COUNT         1298 a          899 c          802 g         1112 t
ORIGIN      
        1 gctttagtgg gttgttcaag caattcgggt gaatccactt ctgcggtaga aacaacagaa
       61 gcaaatgcaa cttggcaatc agcagatagc attcaaactg caccaatgcc tgcctcgatg
      121 aatcaaccta ttgccactca gccaacctat cagcagccga cttactctgc accacaacca
      181 atttcggcag caccagcacc aatgcctgtt tcaagtggta gtgatagctc tcaagttgaa
      241 acagtaggga actgccaagt ggtgcgtgat gcgaataatg ctcctatcta ttcacaaatt
      301 accaaaggct gctacacaga aagtcactat acggtaggta aaagtgatac gctttattta
      361 atcggttatc taaccggcac caatgccaat caaattgcgc agctaaatgg tttaaatacc
      421 ggctctccac tgaaagtcgg gcaagtctta cgtgttcgtt aataggcaga gaaaatgaac
      481 agaacccttt tagcatttgc aatttcttgt gcatttttga ccgcttgtag ctcgcctact
      541 cagccacaag ttgaagatcc aaatatgtta cctgcaggca cattacaacc gattgaaggc
      601 tcaggcgcaa gccaagacag ttacagttgg ccgtctgata ttcaatcaac gtcaatgcct
      661 gacagtatga aataatttta ggacaatcat ttaatgaaaa aacaatttat tcttctccct
      721 ttagtctctg ctgttttggc agcctgttct tcaaacaatc cagcaccggt ggtgaatgct
      781 tccggtaata acgaattaag cccgggcgtg atgcagcctg ttgatgtgag tgcaggtgca
      841 attaattcaa gcggtggctg gcagtctgat attcaatctg caccaatgcc aagctcgatg
      901 aataccactc cggtgcaaac aatgccccag ccggtttcag tgccacaacc tgtggcgact
      961 gtaccgcagc caattaccaa tccggtgatt accgaacaac caccacagcc gacaaccacg
     1021 accaaaatcg tgaaaaaaac gaaaacggtc gagaaaaaag tggatcaaaa ttttgagatc
     1081 ccgcgtgatg ccaacaatgc cccaatgtac aaccaaattc aaaaagggtt ctacgatggc
     1141 tctacctata ccgtacgcaa aggcgacaca atgtttttga ttgcctatat tgtgggtaaa
     1201 gatgtaaaag aaatcgccgc tcttaataat atgagcgaac cttaccaatt aagcgtaggg
     1261 caaaaactca aaaccggcaa atctgcaacg gaaactatta ccgtggaaga aaaagtcacc
     1321 gtaccggttg aaccacaaat tacctaccaa caaggtgcaa acggcacaac ctatgcttct
     1381 ggcggtaata ttactgggcc ggtaaaagca ggcacagcca acgctgatgt cacgccggtt
     1441 aataacggta ttaaagcaac aactgcaact gctccggcag taactgcttc tgtcggtaca
     1501 gtaaatgcaa cagaggaccg taccgcctcc accattcgtg ctatagatgg cacagcaagt
     1561 ggtgccgtac aaagtagtaa tactgtttct gcaccagctt catcaattaa atggcaatgg
     1621 ccaaccagcg ggcgtgtaat ttcaggcttc tcctcagcag aaggaggtaa taaaggcatt
     1681 gatattgccg gcaataaagg acaagatgta cgtgctgccg ccgcaggtaa agtggtatat
     1741 gccggtaatg ccttgcaggg ttacggtaac ttaatcatca tcaaacatac cgatgatttc
     1801 ttaagtgctt atgcacacaa caatactatt gaagttgatg agcaagaaac ggtgaaagcc
     1861 ggtcagaaaa tcgcgacttt aggtagtaca ggaaccaata cgaataaatt acattttgaa
     1921 attcgttaca aaggaaaatc tgtcgatccg gctcgttatt taccaagaaa ataagagtaa
     1981 ctttttagca taatccgacc gcttgtgtac tacaagcggt caatttttag gaaaattatg
     2041 caaatgaaaa aatatatcgc acacatgata agttcgttac tgctgatagc ttgttcggac
     2101 taccgagcaa caccaactag cccaagcgat gcttacactt gctctccttc ttttcgctac
     2161 tctttcttaa aaaaaacgtg cataccttta ttcagtatag ccgacattca attattggat
     2221 ccaaccgagc cttcacatcg catttatatt ttgttatctg aagatcgatt tcaagccgaa
     2281 gtcttctcaa cgaagctctc tgaatcttct atgttattgg aggcggttaa aggaggctat
     2341 atttcaaaag acggaaaagt tcgtttactc aaaaaagctg aaggttggaa aatttactat
     2401 gagtgagact aaaatacgtt gtgattggtg taaaagcagt gaaatttata tgaaatatca
     2461 cgacgaagaa tggggaaaac cggagtttga cagcctcaaa ctgtttgaga aaatctgcct
     2521 tgaagggcaa caagccggac tttcttggat tacggtgttg aaaaaacggg aagcctaccg
     2581 ccaagcattc catcagttta atcctgaaaa aattgccaaa atgggtgaag aagaaattga
     2641 tttgttaatg caaaacacta gcttaatcca ccaccgagca aagttagaag ccattataaa
     2701 aaatgcgaaa gcttatatca caatgcagca aaatggcgaa gacttcagtc ggtttatttg
     2761 gtcttttgtg aattatcagc cgcaaattaa tgatgtacca acacttgaag ctgttcctgc
     2821 tcatacagaa acctcaaaag caatgtcaaa agcgttgaaa aaacgtgggt ttgtgtttgt
     2881 aggcgagact acttgctatg cttttatgca gtcaatgggg ctggttaatg accatttgaa
     2941 taattgcgtg ttcaaacata aataaacaca aaaaattagg cgaccatttg gtcgcctaac
     3001 attaattcag attattgatt gttttgaacg taatcaattg ctgattgaac tgtagtaatt
     3061 ttttcagctt cttcatctgg aatttcgata tcgaattctt cttctaaagc cattactaat
     3121 tcaacagtgt ctaaagaatc tgcacctaaa tcttcaatga atgaagcttc tggttttaca
     3181 tcttctgctt tcgcgccaag ttgatcaaca atgatttttt ttacgcgttc ttcaatgctc
     3241 attttgtttt tcctatagtg aatatcgcat aagtgcgaga gttaaatagt gtaatcgaaa
     3301 taagtttaaa tacaaccttt tataaaaagg tcaaaccaga ctattcctat acaccagaat
     3361 atatctgttt ttccattcta tcattatgtt tgtgtttaat ctatagagat aaatcaaatt
     3421 tcatttgatt accctacttt ccgtaattca tctcgcattt gtgcaatcac tgcttgataa
     3481 tctggctggt caaaaatcgc agaacctgcc acaaacatat ctgctcctgc ggctgcaatg
     3541 tcggcaatgt tatttatttt cacaccacca tcaacctcta agcgaatgtt ataaccgctc
     3601 tcatcaatac gacgacggac ttctttcaat ttatctaagg tggacggaat aaatgattgt
     3661 ccgccaaagc ccggattcac agacatcaat aaaaccacgt caactttatc catcacataa
     3721 tctaaatagc tcaatggcgt tgccgggtta aacaccaagc ctgatttaca acctaaatca
     3781 cggataagct gtaaatttcg atctatatgt tccgttgctt cagggtggaa tgtaatataa
     3841 tccgctccgg ctttggcaaa atccggaatc aaacgctcaa ccggttttac cattaaatgc
     3901 acatcaatcg ggcattgaat gccgtaatca cgtaaggctt tgcagatcgc cggcccgaag
     3961 gtcaaattcg gcacgtagtg gttgtccatt acatcaaagt gaattaagtc tgcaccggct
     4021 tttaacacct ctgccacatc atcacccaag cgagctaaat cggcagataa aatagacggt
     4081 gcaattaaat aaggattctt actcatattt c
//
LOCUS       DANLYI010000194         3990 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.196, whole genome shotgun sequence.
ACCESSION   DANLYI010000194 DANLYI010000000
VERSION     DANLYI010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3990
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.196"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            <1..257
                     /locus_tag="PXD81_002170"
     CDS             <1..257
                     /locus_tag="PXD81_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5538154.1"
                     /translation="IEKISKNNKTQFSSTIRENQINFIRALLYMDYGIETPQEVKNAI
                     NSGELGRKIEKFRRENPNIENDKNFKFPTSVTLSNWYSKA"
     gene            335..586
                     /locus_tag="PXD81_002171"
     CDS             335..586
                     /locus_tag="PXD81_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL5538155.1"
                     /translation="MQLQKTISTFTQPNHRGIYVMTYSEQSYLNVKDVCEMLQLNRST
                     IYRQIKQGIFPKPTKIGKSSRWKFADIKAFLEPKPQEAN"
     gene            586..966
                     /locus_tag="PXD81_002172"
     CDS             586..966
                     /locus_tag="PXD81_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDL5538156.1"
                     /translation="MAGLSIICPFCGNTARIRGSSRPSLLSYQAQLTCSNCGQLKADF
                     VGQLINIKRAVFIECDEANQWDKPENELIKEGKIQGKDNAQRLKELRGSQQDLFAKVP
                     GQLPPEKITPAEKIAKRANLSKTH"
     gene            1277..2338
                     /locus_tag="PXD81_002173"
     CDS             1277..2338
                     /locus_tag="PXD81_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ash family protein"
                     /protein_id="HDL5538157.1"
                     /translation="MKDLSPNQKQYTPFSAIMQKNLMDLLTKSARSPYSFSTVAKSTA
                     GRENPELHNTANSSTPLTRAIFIRSLHTPKERRNKACSSMVACSGQGSPLAVFGSPFV
                     AVFSPRYTLSPCTVRSVADSPLKLTNGLQKMNPSQITGKNRLNSFLFNNNQNDYSLLF
                     TNAKQSTPISAKISKNQDLCLTTLNRSPYASRAFAKSKAECGNSKEHQANNSTPLTRA
                     FFVRSSRTPKECQNKACSSMVACSGQGFALDCLPDVAVFHPVTRYRPTLWKMLAIAPK
                     NQHQELSKMKAFAYSFLCAIRTPETDTYQETVVRIISDSEQNARFQLTADYRLLLPRP
                     VAKIRLNSTAYTVKGGIYA"
     gene            2331..2495
                     /locus_tag="PXD81_002174"
     CDS             2331..2495
                     /locus_tag="PXD81_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5538158.1"
                     /translation="MLNYSDIEQIRYFMDQAEEALDNNEEFKAVAELNTAQQFILALL
                     MNYEQGGENE"
     gene            2488..3123
                     /locus_tag="PXD81_002175"
     CDS             2488..3123
                     /locus_tag="PXD81_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5538159.1"
                     /translation="MNNTTGQATQRNWQEIIANLQRLSESGESTIEIAKRYEAFSKTA
                     EQIQALVNNNGSEAEKEAFFAPVERFYLGLLETETRLKDAIAKDEENAQKLKEQKAAR
                     EAEQKRWDNLPFYAKDKFSDLICIESRETLEDKFFAISCMFDALKAVPNLLEIGLREE
                     GKDSVIDRLAEMSKLYLLFVDEQFSLLANEIQSGKYGKLDLNGSQNKGDLC"
     gene            3117..3335
                     /locus_tag="PXD81_002176"
     CDS             3117..3335
                     /locus_tag="PXD81_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5538160.1"
                     /translation="MLTHRITLPDGTEIEFFAETDKETFAEIQRDLEGLKLPTFAEFV
                     AQERKKHRAKQAVKKARKIAKLARRKNR"
     gene            3477..3962
                     /locus_tag="PXD81_002177"
     CDS             3477..3962
                     /locus_tag="PXD81_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5538161.1"
                     /translation="METALQLAQHLTGWTEEENSQAILKNFLDWKETFGEKTREETKI
                     VETLLDWINENEASFIEYPFNQEQRTPPKVAGYRILANNSTKTNEYFFIYPKQFKETL
                     AEFPLLMACTKLHEAGILHKPPRPEKGYEFQYTIAPKITGGSKKRAYKIVPIALEEEN
                     E"
BASE COUNT         1444 a          840 c          765 g          941 t
ORIGIN      
        1 aaatagaaaa aatatcaaag aataacaaaa ctcaattcag ctcaacaata cgagaaaacc
       61 aaatcaattt tattagagcc ttgctttata tggattatgg gatagaaacg cctcaagaag
      121 taaaaaacgc cattaattca ggggaactag gcagaaaaat agagaaattt aggagagaaa
      181 accccaatat agagaacgat aaaaatttca aattccctac aagtgtaacc cttagcaact
      241 ggtattcaaa agcctaaaat attggaaatt tccgaatcta ctttggaaat ttccaaactt
      301 ctaaaaccta ctcacctcac aatctccccc aacaatgcaa ctacaaaaga caataagcac
      361 attcacgcaa ccaaaccaca gggggattta tgtaatgacc tattcagaac aaagctattt
      421 aaacgtgaaa gatgtatgcg aaatgctaca actcaaccgc tcaactattt atcgacaaat
      481 taagcaaggg attttcccta agccaactaa gatcggaaaa tcaagccggt ggaaatttgc
      541 cgacatcaaa gcatttttag agccaaaacc acaggaggcg aactaatggc agggctttct
      601 attatttgcc cgttttgtgg caataccgca cgaattagag gcagttctcg ccctagcctt
      661 ttaagttatc aagcacagct aacttgttca aattgcggac aattaaaagc cgactttgta
      721 gggcagctaa tcaacatcaa acgggccgta tttattgagt gtgatgaagc aaatcaatgg
      781 gataaacccg aaaatgaact aatcaaggag ggaaaaattc aaggcaaaga caatgctcaa
      841 cgcctcaaag aactaagagg ctcacagcaa gatttatttg caaaggtgcc ggggcaacta
      901 ccgcccgaga agatcactcc ggcggagaaa atcgccaagc gggcaaacct ttcaaaaacg
      961 cattaaacag ggggataaat ggcaacaaaa aagccccgca aggcaaacca tacggagcta
     1021 ttaaaatcgc ataatgcgaa agatatgcaa agaaattaaa gcaggcttga cgatttctga
     1081 aggtgttaga ttctttctaa agtctttagg gataggttta tttctagcat tgttttttta
     1141 ttttttgccg tatttagcag aatttataat cgccgtaaaa aaacaactag caaaaaaatc
     1201 ggcttacttt caatgataaa agcaagccga aatgacaatc caaattttta actttaaata
     1261 aagagtacaa gaagaaatga aagatttatc tccaaatcag aaacaatata cgccattttc
     1321 ggctataatg caaaaaaatt taatggattt attgacaaaa tccgcccgtt cgccttattc
     1381 tttctctacc gttgcaaaat caacggcagg gcgtgagaac cctgaactac ataatacggc
     1441 gaacagtagc acgcctttaa cccgtgctat ttttattcgt agcttacaca cacctaaaga
     1501 acgccgaaat aaggcgtgtt cgtctatggt agcgtgtagc gggcaaggtt cgcccttggc
     1561 tgtttttggt tcgccgtttg tagcagtttt ctcaccccgt tacacgctat cgccctgcac
     1621 cgtgagaagt gtagccgata gtcctttaaa attaacaaac ggactacaaa aaatgaaccc
     1681 tagccaaatt acaggcaaaa accgcctaaa ctcttttcta tttaacaata atcaaaatga
     1741 ttattcgcta ttatttacta atgcaaaaca atcaaccccg atttctgcta aaataagcaa
     1801 aaatcaagat ttatgcttga caaccctcaa tcgttcgcct tatgctagcc gtgcttttgc
     1861 aaaatcaaaa gccgagtgtg gaaactcgaa agaacatcag gcgaacaata gcacgccttt
     1921 aactcgtgct ttttttgttc gtagctcacg cacacccaaa gaatgccaaa ataaggcgtg
     1981 ttcgtctatg gtagcgtgta gcgggcaagg tttcgccctt gactgcttac ctgatgttgc
     2041 agttttccac cccgttacac gctatcgccc tacattgtgg aaaatgttag cgatagcccc
     2101 taaaaatcaa catcaggagc tatcaaaaat gaaagcattc gcttattcat tcctttgtgc
     2161 tattcgcaca cccgaaacag atacttatca agaaaccgta gtacgcatta tttcagacag
     2221 cgaacaaaac gcccgttttc agcttacagc cgattatcgc ttactactcc cccgccctgt
     2281 tgcaaaaatc cgcctaaatt ctaccgctta cacggtgaaa ggggggattt atgcttaatt
     2341 attccgatat tgaacaaatc cgctatttta tggatcaagc cgaagaggct ttagacaata
     2401 atgaagagtt caaagccgta gcagaactta acacagcaca acaatttatt ctggctttat
     2461 taatgaacta cgaacaaggg ggtgaaaatg aataatacaa caggacaagc tacgcaaaga
     2521 aactggcaag aaattatcgc taatttgcag agactcagcg aatccgggga aagcacaatc
     2581 gaaatcgcta aacgctatga ggcattttct aaaaccgccg agcaaatcca agccctagtg
     2641 aataacaacg gcagcgaagc cgaaaaagag gcgttttttg cccctgttga gcgtttttat
     2701 cttggcttac tagaaaccga aacacgctta aaagatgcca tagccaaaga cgaagaaaac
     2761 gcccaaaaat tgaaagagca aaaagcagct agagaggcag agcaaaaaag atgggacaac
     2821 ctacctttct acgccaaaga caaattcagt gatttaattt gcattgaaag ccgagaaacg
     2881 cttgaagata aattctttgc gatctcttgt atgttcgatg ccctaaaagc cgtacctaat
     2941 ctattagaaa taggcttgcg tgaagaaggc aaagacagtg ttatagaccg attagccgaa
     3001 atgagcaaac tctatttact gtttgtagat gagcaatttt cactactggc aaacgaaatt
     3061 caaagcggta aatatggcaa gttagaccta aacggctcac agaataaggg ggatttatgc
     3121 taacgcaccg cattacactc cctgacggca cagaaattga attttttgca gaaaccgaca
     3181 aagaaacatt tgcagaaatt caaagggact tagagggctt gaaattgcca acctttgccg
     3241 agtttgttgc ccaagagcgg aaaaaacacc gagcaaaaca agcggtcaaa aaagcccgaa
     3301 aaattgcaaa attagcaaga cggaaaaacc gctagcacct atcaaaaaga cttattattg
     3361 cagaaaatca cgctttaata aaaacgacct ataaagcaat ccgcaagaaa tggttaagtt
     3421 taactgaaaa tatgagcgga caggttcagc gggtagcgtg cgatagattc gcaataatgg
     3481 aaaccgcctt gcagttagcc caacatttaa caggctggac agaggaagag aacagccaag
     3541 cgattttaaa aaacttccta gactggaaag aaactttcgg cgaaaaaaca agagaagaaa
     3601 ctaaaatagt agaaaccttg ctagattgga taaatgaaaa tgaggcgagt tttatcgagt
     3661 acccattcaa ccaagagcaa agaacaccgc ctaaagttgc cggctataga atacttgcga
     3721 ataacagcac taaaacaaat gagtactttt tcatttaccc taagcaattt aaagaaacgc
     3781 tggcagaatt tccgctattg atggcttgca ccaagttaca cgaggcaggg attttacata
     3841 agccaccacg cccagaaaaa ggctatgaat ttcagtacac aatagcccct aaaataacag
     3901 gagggagcaa aaaaagagct tataaaatcg taccgatagc attggaagaa gaaaacgagt
     3961 aattacagca aaagcaagag tttcaattct
//
LOCUS       DANLYI010000195         3981 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.197, whole genome shotgun sequence.
ACCESSION   DANLYI010000195 DANLYI010000000
VERSION     DANLYI010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3981)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3981)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3981
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.197"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            complement(106..1746)
                     /gene="pgi"
                     /locus_tag="PXD81_002178"
     CDS             complement(106..1746)
                     /gene="pgi"
                     /locus_tag="PXD81_002178"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013527751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HDL5538162.1"
                     /translation="MQKINPVQTFAWNALEQHKADNLSIPQLFAEDPNRFDKYSLRFE
                     DQLLVDFSKNAINEKTLTLLRQLANECGLESAKNAMFSGKKINRTENRAVLHVALRNR
                     ANTPIAVDGKDVMPEVNAVLAKMKDFCERVISGEWKGYTGKTITDVINIGIGGSDLGP
                     YMVTEALRPYKNHLNMHFVSNVDGTHIAEVLRKVNPETTLVLVASKTFTTQETMTNAL
                     SARKWLLDSAKDESAVAKHFVALSTNAKEVEKFGIDTANMFEFWDWVGGRYSLWSAIG
                     LSIALSLGFENFEQLLAGAHAMDKHFLITPIEKNIPTTLALVGIWNNNFLGAESEAIL
                     PYDQYMHRFAAYFQQGNMESNGKFVGRDGKPVTHQTGPIIWGEPGTNGQHAFYQLIHQ
                     GTKLIPCDFIAPAQTHNPLSDHHNKLLSNFFAQTEALAFGKSKETVEKEFLDAGKTLE
                     EVAEIVPFKVFSGNKPTNSILVQKITPYSLGALIAMYEHKIFVQGVLFNIYSFDQWGV
                     ELGKQLANRILPELETDEKINSHDSSTNGLINQFKAWR"
     gene            complement(1779..2027)
                     /locus_tag="PXD81_002179"
     CDS             complement(1779..2027)
                     /locus_tag="PXD81_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory factor UbiK family protein"
                     /protein_id="HDL5538163.1"
                     /translation="MLTPKNLEAIAQQLHNALPQSLKNVGNDLDEKFKQILQAQLARL
                     DVVTREEFDVQSQVLLRTREKLTELEKRLDELTQRTDE"
     regulatory      2151..2195
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            2199..2867
                     /gene="queC"
                     /locus_tag="PXD81_002180"
     CDS             2199..2867
                     /gene="queC"
                     /locus_tag="PXD81_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HDL5538164.1"
                     /translation="MTHQKPKAVVIFSGGQDSTTCLFQAIAEFGKENVEVVTFQYGQR
                     HSIELEKARWISNDLGVKQTLIDTSVIKAITTNALMDNNAEIQQQGSTPNTFVDGRNA
                     LFLLYTAIYAKGQGIQTIFTGVCETDFSGYPDCRDVFVKSMNVTLNLAMDYNFNIRTP
                     LMYLTKKETWALADKLGAFDYIRDHTHTCYLGVEGGCHDCPSCVLREKGLNEYLAERE
                     VSNV"
     gene            2860..3282
                     /gene="queD"
                     /locus_tag="PXD81_002181"
     CDS             2860..3282
                     /gene="queD"
                     /locus_tag="PXD81_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005597968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HDL5538165.1"
                     /translation="MFKIAKEFSFDMAHMLDGHDGKCQNLHGHTYKLQVEVAGELFQE
                     GAKRGMVMDYSDLKAVVKQHILEPMDHAFIYDLQSERESQVAQLLIDLNSKVYGIPTR
                     TTAEEMAKYMFEKLEKVGLPVSLIRLWETPTSYCEYSK"
     gene            3282..3908
                     /locus_tag="PXD81_002182"
     CDS             3282..3908
                     /locus_tag="PXD81_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HDL5538166.1"
                     /translation="MAELISNPRFPIVEIFESLQGEGFNTGMPAIFVRFGKCNLTCPW
                     CDTNYNQFEQWTLSDILAKVRSYSAKNVIITGGEPTIQPNLSLLLEQFKKEGYFLAIE
                     TNGLKEVPAQIDYIATSPKRMYQEKYQRRCIEFANEVRIVVDGEVQGFCEQIESQIKA
                     EHYYLSPCEVEGKMNLLETITQIGLLNQRPNKPKWQLSIQTHKIVGIE"
BASE COUNT         1189 a          746 c          846 g         1200 t
ORIGIN      
        1 aaactgcttg tgtagaaatg gcgcttagtt cagtcctcag ctaaaatttt agaaaaaaag
       61 catagagatt ttttacctct atgcttttta ttgtattgac tatatttaac gccaagcctt
      121 gaattggtta attaaaccat ttgtggagct gtcgtggctg ttgatttttt catcggtttc
      181 gagttcgggt aaaatgcggt ttgcaagctg tttgcctaac tccacgcccc attggtcgaa
      241 actgtaaatg ttgaacagca cgccttgcac gaagattttg tgttcataca ttgcaattaa
      301 tgcccctaag ctgtacgggg tgattttttg caccaagata gagttggtcg gtttgtttcc
      361 gctgaacact ttaaacggca cgatttcagc tacttcttcc aacgttttac ctgcatctaa
      421 aaactctttt tcaactgttt cttttgattt accgaaagct agtgcttcag tttgagcaaa
      481 gaagttagaa agcagtttgt tgtgatgatc gcttaacgga ttgtgagttt gtgccggtgc
      541 gataaaatca caaggaatta attttgtgcc ttgatgaatg agttgataga atgcgtgctg
      601 accgttagtt cccggttcgc cccaaataat cggacctgtt tggtgagtaa ccggtttgcc
      661 gtcacgcccc acaaatttac cgtttgattc catattgcct tgttggaaat aagccgcaaa
      721 gcggtgcata tattggtcgt aaggtaaaat cgcttctgat tcagcaccta agaaattgtt
      781 attccaaatg ccgactaaag ctaacgtagt tggaatgttt ttctcaatcg gcgtgattaa
      841 gaaatgctta tccattgcgt gagcaccagc aagtagctgc tcgaagttct caaaacctaa
      901 tgaaagggca atcgaaagcc cgatggcaga ccataatgag taacgaccgc caacccaatc
      961 ccagaattca aacatattag cggtatcaat accgaatttt tccacttctt tggcattggt
     1021 tgaaagtgcc acgaaatgtt ttgctaccgc agattcatct ttggcagaat caagtagcca
     1081 tttgcgagct gaaagggcat tggtcatcgt ttcttgagtg gtaaaggttt ttgaagccac
     1141 taacactaac gtggtttcag gattaacttt acgcaacact tcagcaatat gcgtgccgtc
     1201 cacgttagac acaaagtgca tattaaggtg atttttataa gggcgtaatg cctctgttac
     1261 catataagga cctagatccg agccaccaat accgatattg atcacatcag taatcgtttt
     1321 gccggtatag cctttccatt cgccggaaat aacacgctcg cagaaatctt tcattttcgc
     1381 taacacggca ttcacttccg gcattacatc tttgccatcg actgcgattg gtgtattggc
     1441 acggttacgc agtgcaacgt gcaatacggc acggttttca gtgcggttga tttttttgcc
     1501 ggagaacatc gcattttttg ctgattccaa accacattca tttgccagtt ggcgaagtaa
     1561 ggttaaggtt ttttcgttga tcgcattttt tgaaaaatcg actaataatt gatcttcaaa
     1621 acggagggaa tatttgtcga aacggtttgg atcttcggca aaaagttgag gaatagaaag
     1681 gttgtctgct ttatgttgct ctaaggcatt ccaagcaaaa gtttgcacag ggttaatttt
     1741 ttgcatctga gtttccttat actaaattaa aaaatcgatt attcatctgt tctttgagtc
     1801 agttcatcaa gacgtttttc taattcggtt aatttttcac gagtgcggag taacacttga
     1861 gattgaacat caaattcttc acgagtgaca acatcaagac gggctaattg ggcttgaagg
     1921 atttgcttaa atttttcatc tagatcatta ccgacatttt ttaagctttg cggtaaggcg
     1981 ttatggagtt gttgagcaat cgcttctaag ttcttcggcg tgagcatatt taaattcctt
     2041 actataaatg agtatcccaa ttttattgaa ttttgggttt aagagaaagc aaattctttg
     2101 taaaatatca cctttcgcaa aatacaagca aaaaagaccg cttgtatttt ttatttgtgg
     2161 ttcgtaaacc tcccacacaa aaaaactaag gacacaaaat gactcatcaa aaaccaaaag
     2221 ccgttgtgat tttttccggc ggtcaagact ctaccacttg tttattccaa gctattgctg
     2281 aatttggcaa agaaaatgtg gaagtcgtta cctttcaata tgggcaacgc cattcgattg
     2341 aacttgaaaa agcacgctgg atttctaacg atttaggcgt caaacaaacg ctgattgata
     2401 cttcggtgat taaagcgatt actaccaatg cgttaatgga taataatgct gaaattcaac
     2461 aacaaggcag cacacccaat acctttgtgg atgggcgtaa tgccttattt ctgctttata
     2521 ccgccattta tgccaaaggg cagggcattc aaactatttt taccggcgtg tgtgagaccg
     2581 attttagtgg ttaccctgat tgccgagatg tatttgtaaa atcaatgaat gtaacgctaa
     2641 atttagcgat ggactataat ttcaacattc gcacgccatt gatgtattta actaaaaaag
     2701 aaacttgggc attagcagat aagctgggtg cttttgatta tattcgagac catacccaca
     2761 cctgttattt aggcgttgaa ggtggttgcc acgactgccc gagttgcgta ttgcgagaaa
     2821 aaggcttgaa tgaatattta gcagaaaggg aggtaagcaa tgtttaaaat tgctaaagaa
     2881 tttagttttg atatggctca tatgctagat ggacacgatg gcaaatgcca aaacttacac
     2941 ggacacacct acaaattgca agttgaggtg gcaggtgagc tatttcaaga gggagctaaa
     3001 agaggaatgg taatggacta ttcggatcta aaagcagtgg taaaacaaca tattttagag
     3061 ccgatggatc acgcttttat ttacgactta cagagtgagc gtgaaagcca agtagcacaa
     3121 ttattaattg atttaaactc caaggtttat ggtatcccaa ctcgtaccac agccgaagaa
     3181 atggcgaaat atatgtttga aaaattagag aaagtgggct taccggttag cctaattcgc
     3241 ttgtgggaaa cgcctacttc ttattgtgag tacagcaaat aatggcggag ttaatttcaa
     3301 accctcgttt tccgattgtt gagatctttg aaagcttaca aggggaaggc tttaataccg
     3361 gaatgccggc tatttttgta cgttttggca aatgcaacct cacttgcccg tggtgcgata
     3421 ccaattacaa tcaatttgag caatggacgt taagcgatat tcttgccaaa gtaagaagct
     3481 attctgcgaa aaatgtgatt attaccggag gcgagccaac gattcagcct aatttatcgt
     3541 tattattaga acaatttaaa aaagagggat attttttagc gatcgaaacc aacggcttaa
     3601 aagaggttcc ggcacaaatt gattatattg cgaccagccc aaagcgaatg taccaagaaa
     3661 agtatcaacg ccgttgcatt gaatttgcga atgaagtccg tatcgtcgtt gatggagaag
     3721 tacagggttt ctgtgagcaa atagaaagcc aaattaaagc tgagcattac tatctttcac
     3781 cttgtgaggt agagggaaaa atgaacttgc tggaaaccat cacccagatt gggttattaa
     3841 atcagcgtcc aaataagcct aaatggcagc taagtatcca aacgcataaa attgtgggga
     3901 ttgaataagt agaagttaga agatgatttg taaaagatca gacttgatct gacaattcac
     3961 tctaaaaagt gagagtttcc c
//
LOCUS       DANLYI010000196         3979 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.198, whole genome shotgun sequence.
ACCESSION   DANLYI010000196 DANLYI010000000
VERSION     DANLYI010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3979
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.198"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            34..1509
                     /gene="mepM"
                     /locus_tag="PXD81_002183"
     CDS             34..1509
                     /gene="mepM"
                     /locus_tag="PXD81_002183"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HDL5538167.1"
                     /translation="MAVFLLAVFSLFVDIVLALKDQSSINNTSSQEDIIAIDKQEKYQ
                     SINTNLAENNTLHQEQHSSFINYVTDLLTQSALVIEHDDEIVDDQTATSYEDELVGQD
                     DEVEEGIETVENQVPGEKLPEEAEKALEDLLDVADQALRIQNQFSYTVARGDKLKDVL
                     EQSGLTASVAKNLIKQHSALAQLEPGQQFYWVLDNDGELEYMNWLVSEKEERVYERKE
                     NGKFSFQRFEKKGEWKLDVVRGTIQNSFAVSLKAVGLSDRQINQLAVGLQSQIATNKL
                     KKGDRFAILIKREYIDGKVTDLGNVEGIHVISSKKSYYAIQADNGRYYSRYGETLSGG
                     FARHPLLFAARVTSSFNPKRRHPITGRISPHKGVDYGVPIGTPIIAPSDGVVEHVAYQ
                     ARGAGRYIRIKHGHITTVYMHLSKTLVKPGQSVKKGERIALSGNTGGSTGPHLHYEFH
                     INGRPVNPITVKLPGSNSGMGNKERQAFLNKAKNVESRLKM"
     gene            1519..2202
                     /gene="dsbC"
                     /locus_tag="PXD81_002184"
     CDS             1519..2202
                     /gene="dsbC"
                     /locus_tag="PXD81_002184"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HDL5538168.1"
                     /translation="MKKNLITLGLMCLSTITFASDAKLKSQLEKIGATDVKISDSPLT
                     GFKMATSNEGVVYISQDGRYVIQGKVFELKNGKAIDITNQGLLAELNSYTNEMIVYPA
                     KNEKHVITVFMDITCHYCHLLHTKIKEYNDLGITIRYLAFPRGGMNTQTAKQMEAIWT
                     SADKVKALNDAEDGKLPTQIKTPDIVKKHYQLGIKFGVTGTPNMVTSEGELIGGYVEP
                     KELAKMLSE"
     gene            2287..>3979
                     /gene="recJ"
                     /locus_tag="PXD81_002185"
     CDS             2287..>3979
                     /gene="recJ"
                     /locus_tag="PXD81_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005616987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HDL5538169.1"
                     /translation="MEKIIKRRPLLTTEIVSTNPLLNRIYQSRGIKNTQELEHNLKNL
                     HRPQQLANIEQAVDLLLQAFREKSRIIIVGDFDADGATSTSLALIALRQLGFDKVDYL
                     IPDRFSQGYGLSVAVAEMVLAKGADLVLTVDNGISSTEGIALLKSHQIQVLVTDHHLP
                     PESLPNADALVNPNLAFCEFPSKSLAGVGVIFYVMMALRSRMREQHLFENKPEPNFAE
                     LLDLVALGTVADVVPLDQNNRILVHQGLQRIRSGHCRLGIKALAEVGKRDLSTLQAAD
                     LGFAIAPRLNAAGRLENMSLGVELLICENMELARRLALDLDSLNQTRKEFEQEMKAEA
                     LSICANLPSLAQSEQAHGIVLYQADWHQGVIGILASRIKDQFNRPVIAFAQESEESEY
                     LKGSARSINGVHMRDLLEHIDMRHPDLIEKFGGHAMAAGLTIHQSKLDLFKQIFDQSI
                     NAIIEPEQLQGIIYTDGELNALEFTLANAEMIRQGGPWGQHFPEPSFEGEFSILQQKL
                     LAGKHLKLMLQSEKGQLFDAIWFNVDVRAFPDLSIKKARLVYRLDINEFRGEKSLQ"
BASE COUNT         1331 a          642 c          875 g         1131 t
ORIGIN      
        1 agagatcgta agcgacataa aaaacatttt aagattgcgg ttttcttatt agcggtattt
       61 agtctatttg ttgatattgt tctcgcacta aaagatcaat cctcaatcaa taatacatct
      121 tcacaggaag atattattgc cattgataaa caggaaaaat atcagagtat caacactaat
      181 ttggcggaaa ataatactct ccatcaggag caacattcca gttttattaa ttatgtgact
      241 gatttactga ctcagtcggc attagtaatc gaacatgacg acgaaattgt tgatgatcaa
      301 acagcgactt cttatgaaga tgaattagtt gggcaagacg atgaagtaga agaagggatt
      361 gaaacggttg aaaatcaagt tcctggggaa aaactcccag aagaagctga aaaggcatta
      421 gaagatttac ttgatgtggc tgatcaagca ctacgaattc aaaaccaatt cagttatacc
      481 gtagctcgag gtgataaact taaagatgta ttagagcaat caggcttaac tgcatccgtt
      541 gcgaaaaatt taattaagca acattcggca ttagcacaat tagaaccggg tcaacaattt
      601 tattgggtat tagataatga cggtgaatta gaatatatga actggctggt ttccgaaaaa
      661 gaggaacggg tttatgagcg aaaagaaaat ggtaaatttt cttttcaaag atttgagaaa
      721 aaaggggaat ggaagttaga tgttgtaaga ggaactattc agaatagttt tgcggttagc
      781 ttaaaagccg ttggcttatc agaccgccaa attaaccagc tagcggttgg attgcagtca
      841 caaattgcga cgaataaatt aaaaaaaggg gataggtttg caattctgat taagcgggaa
      901 tatattgacg gcaaagtaac ggatttagga aatgtggaag gaatccatgt aattagcagt
      961 aaaaaaagtt attatgctat tcaagcagat aatggacgct attatagtcg atatggtgaa
     1021 acattaagtg gaggatttgc tcgtcatcca ttattatttg cagcaagagt aacttcttct
     1081 ttcaacccca aacgcagaca ccctattaca gggagaatta gtccgcataa aggggttgac
     1141 tatggcgtgc caataggtac tccgatcatc gcaccaagtg atggtgtcgt agagcatgtg
     1201 gcatatcaag cccgtggagc aggacgttat attcggataa aacacggaca tattacgact
     1261 gtttatatgc acctcagcaa aacgttggta aaaccagggc agagtgttaa aaaaggggag
     1321 cggatagcat tatcgggtaa tacaggcggt tcaaccgggc ctcatttaca ctatgaattc
     1381 catattaatg gtcgaccggt caacccgatt acggtaaaat tgcctggctc aaattcaggt
     1441 atgggtaata aagaacgtca agcattctta aataaagcga agaatgtaga aagtagatta
     1501 aaaatgtaag gattctcaat gaaaaaaaat ctaatcactt taggattaat gtgtttatca
     1561 accatcacgt ttgcttcaga tgcaaaacta aaaagccagc ttgaaaaaat tggagccaca
     1621 gatgttaaaa tttcagattc tccattaaca ggctttaaaa tggcgacttc aaatgaaggt
     1681 gttgtatata tcagccaaga cggtcgttat gtaattcaag gtaaagtatt tgagctaaaa
     1741 aatggtaaag cgattgatat tactaatcaa ggattgttgg cagaattgaa ttcatacact
     1801 aatgaaatga tcgtatatcc agcgaaaaat gagaaacatg tgattacggt gtttatggat
     1861 attacttgcc attattgcca tttactacat actaaaatca aagaatataa tgatttaggt
     1921 attaccattc gctatttggc attcccgcgt ggtggaatga atacccaaac agcaaaacaa
     1981 atggaagcta tttggacatc agcagacaaa gtaaaagcac tgaatgatgc tgaagatggt
     2041 aaattaccaa cccaaataaa aacgccggat attgtgaaaa aacattacca attagggatt
     2101 aaatttggtg tgacaggtac cccaaatatg gtaacttcag aaggtgagtt gattggtggt
     2161 tatgtagagc ctaaagaatt agcgaaaatg ttaagtgagt aattcattaa cttgaataca
     2221 agcggtcttt ttttgtaaaa tttttgcaaa agaagaccgc ttgttgtttc attaaagaag
     2281 agtattgtgg aaaaaataat taaaaggcgc ccattattaa caacggaaat agtctctaca
     2341 aacccgttat taaatcgaat ctatcaaagt cgaggcataa agaatacgca agaactagag
     2401 cataatttaa agaatttgca tagaccacag caacttgcca atattgagca agccgttgac
     2461 ttgctattac aagcctttcg tgaaaaaagc cggatcatta ttgtgggtga ttttgatgca
     2521 gatggtgcaa ccagtacatc gcttgccctt attgcgttac gccaattggg tttcgataag
     2581 gtagattatt taattccgga tcgtttttct caggggtacg gattgagtgt agcggttgct
     2641 gaaatggtat tagcaaaagg ggcggattta gtcttaacgg tagataatgg aatttcttcg
     2701 actgaaggca ttgctctgct aaaaagccat cagattcaag ttttggttac cgatcatcat
     2761 ttaccgccag agagcctacc caatgcagat gcactggtaa accccaattt agctttttgt
     2821 gaatttccgt ctaaatcttt agccggggta ggcgtgatct tctatgtaat gatggcattg
     2881 cgttctcgta tgagagaaca acatttattt gaaaataaac cagagccaaa ttttgctgaa
     2941 ttactggatt tagtcgcttt aggcacagtg gctgatgtag tgccgttaga tcaaaataat
     3001 cggatattgg tgcatcaagg gttacagcga atccgttcag ggcattgccg tttagggata
     3061 aaagctttag cggaggtggg caagcgagat ctttctactt tgcaagcagc agatttgggt
     3121 tttgctatcg caccacgttt gaatgcagct ggtcggttag aaaatatgtc tttaggggtt
     3181 gagttactaa tttgtgaaaa tatggaatta gctcgccggc ttgcgcttga tcttgattct
     3241 cttaatcaaa cccgaaaaga gtttgaacaa gaaatgaaag cagaagcact gtcgatttgt
     3301 gcgaatttgc caagtcttgc gcagtctgaa caggcacacg gaatagtgtt atatcaagca
     3361 gattggcacc aaggtgtaat tgggatctta gcttctcgca ttaaagatca atttaaccgt
     3421 cctgtcattg cttttgcaca agaaagtgag gaaagtgaat atctaaaagg ttctgctcgc
     3481 tcgatcaacg gagtacatat gcgagattta ctggaacata tcgatatgcg acatccagac
     3541 ttgattgaaa aatttggtgg acacgcaatg gcggctggtt tgactatcca tcaatcaaaa
     3601 ttagatttat ttaaacagat atttgaccaa tctattaatg caattatcga gcctgagcaa
     3661 ttacaaggca tcatttatac cgatggtgag ttaaatgcac tagagtttac tttagccaat
     3721 gccgagatga ttaggcaagg tgggccttgg ggacaacatt ttccagaacc tagttttgaa
     3781 ggggagttta gcattctcca acaaaaatta ctcgcaggta aacatttaaa attaatgttg
     3841 caatcagaga aagggcaact atttgatgca atttggttta atgtggatgt aagggcgttt
     3901 cctgacttat cgatcaaaaa ggcaagatta gtttataggt tggatattaa tgaatttcga
     3961 ggagagaaat ctttgcaat
//
LOCUS       DANLYI010000197         3977 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.199, whole genome shotgun sequence.
ACCESSION   DANLYI010000197 DANLYI010000000
VERSION     DANLYI010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3977
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.199"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            88..2583
                     /locus_tag="PXD81_002186"
     CDS             88..2583
                     /locus_tag="PXD81_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail length tape measure family protein"
                     /protein_id="HDL5538170.1"
                     /translation="MSQAVFYLNNFKELFMTVEVGGVVITADLEFENLLRDTQKVDKA
                     MDDIGKSTLKAEKGLKGLETQATKTAIAVNQATNKMKNIRGVAGQLGYQIQDISIMLQ
                     MGSNASTVIAQQGSQILSIFGPMGAVAGAILAISGAIGGALLPSLFDSTDATELLAAA
                     QKNLSEVVTQTESGVSALSAKIIKLAKVSEDAAKAKIAVAMSDAKIAIKAAGDSSQKA
                     FEQFDGFFGSFTNSSQSIRNAIDELKRYESKGKDVGAVIKELGGTYGGAIAEVNALDS
                     VTSEMSKTLGITQSEALGLIKLLAQLEQDKSPDNIKLLAIALSDLSDKNGWANEELNK
                     LTRTINDNSVSALDAETSVRLLESAIKDLQNAIQNSQGALDGNVAKMNQLADAAERKA
                     ATIGKTERETAKYAATLLATTDEAKKNLDSTLSRIDASYDQIEAYQAEQKALQDKKTA
                     DAKAAREAEAAAKKSAQAKKQVVEQLRQMATQYEIAVLRQKGMHLEAVKLEAGMRLGA
                     AATKEQREEAEKLAQSIYGATAASQNFNAIQAQASPVTALDQRHQQQLAQIEEYKILY
                     PQKIMEAEAARAAIEEQYRQQRMEAQWEEWKQASDGARMFGDAVDAVGSSATSTITGL
                     LNGTMSVRDAFASIANTILNSVVQSLVEMGMAQVKQMIMGQTAAKASMAAQMVQAKAL
                     AASFAPAASMVSLATQGANAIPAQAAITSTMSMAQVAAVTGRKLGGPVSARQMYRVGE
                     NNQPEIFKASNGMQYMIPGQSGRVFSNKQSQGQGGGESKTVAVIVNQTNHFSSDEASA
                     NQAAVAKALSNQIKASVRLELQSQMRAGGVLAR"
     gene            2587..2916
                     /locus_tag="PXD81_002187"
     CDS             2587..2916
                     /locus_tag="PXD81_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL5538171.1"
                     /translation="MQTFNFKVEQDYTVQNEPSVVILKFADGYEQTAPKGLNHNLKKY
                     QGITVKANKAEAVKIQQFLNAHGGYKKFNWRDRTSDETVTVRCRSWSSTKLGAVIEFT
                     LNFDEVI"
     gene            2916..3386
                     /locus_tag="PXD81_002188"
     CDS             2916..3386
                     /locus_tag="PXD81_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HDL5538172.1"
                     /translation="MPKAPPKKMASELIKLEQDALIELWEIDLTHIYSSSNPNQRGEI
                     FRFHNGVSQTQQNIWWQGNEYQAYPIAADGFEISGQGPSNRPTLTVSNLYGVVTVLVE
                     DFGQPVEAKVSSEETVDGNIQMTVELLKKMDQIADQRYRRNQLNDLRTGGTLSK"
     gene            3394..>3977
                     /locus_tag="PXD81_002189"
     CDS             3394..>3977
                     /locus_tag="PXD81_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HDL5538173.1"
                     /translation="MPISISNQMKLDLAKLEQNAMLDLYEVDLRKLQDKNGNAGSVYR
                     FYSGLNELKTSIVWQGRTYDPYPIEATGFERNSSGPSNRPTLTLSNLFGLVTGIANQF
                     DECIGAIVRRHQVYAQYLDAVNFAGGNAKADPNQEIISHFVIEQLSSLTRETATFTLA
                     LPIETDNAKIPSRIIMADTCTWIYRSAECGYTGK"
BASE COUNT         1325 a          798 c          920 g          934 t
ORIGIN      
        1 atgtaacaaa aatatagatt aaattgtatt taaccaatct attgctagat aaattgtaaa
       61 aaaatcacag aaataaccgc ttgtgctatg tcacaagcgg ttttttattt aaataatttt
      121 aaggaattgt ttatgacggt cgaagttggc ggagttgtaa ttaccgcaga tctagaattt
      181 gaaaatctgc taagagatac tcaaaaagtt gataaagcga tggatgacat cggaaaatca
      241 acattaaaag cagaaaaagg gctgaaaggg ctagaaacgc aggcaaccaa aacggcgata
      301 gcagtaaatc aagccacaaa caaaatgaaa aatatccgtg gagtagcagg tcaacttggt
      361 tatcaaattc aagacatctc aataatgctt caaatgggtt ctaacgcttc aaccgttatt
      421 gctcagcaag ggtcgcaaat cttaagtatt ttcggtccaa tgggagctgt tgcaggggcg
      481 atcttagcta ttagtggtgc tattggtggg gcgttactcc ctagtttgtt tgattcaact
      541 gatgcaactg agcttttggc agcagctcaa aaaaatctca gcgaagtagt tactcaaacg
      601 gaaagtggtg tttccgccct ttctgcgaaa atcattaaat tagcaaaagt aagcgaagat
      661 gcagcaaaag caaaaattgc agtagctatg agtgatgcaa aaatagctat taaggcagca
      721 ggggattcat cacaaaaggc atttgagcaa tttgacggct tttttggatc gtttacaaat
      781 tcaagtcaaa gtattcggaa cgcaattgac gaattaaaac gctatgaatc aaaaggtaaa
      841 gatgttggtg ccgtaataaa agaacttgga ggcacatatg gtggtgcaat tgccgaagtt
      901 aatgcgctgg attctgtgac aagcgaaatg tctaaaacgc ttggtattac tcaatctgag
      961 gctcttggtt tgataaaact gttggctcaa ttagagcaag acaaaagccc tgataatatt
     1021 aaattactcg cgatagcttt atctgactta agcgataaaa atgggtgggc gaacgaagag
     1081 ctaaataagc taaccagaac gataaacgat aattcagtat ctgctttaga tgccgaaaca
     1141 tcagtaagac tcttagagtc tgctataaaa gacttgcaaa atgcaatcca aaactcccaa
     1201 ggtgcattgg atggcaatgt agcaaaaatg aatcaacttg cagatgcggc agaaagaaaa
     1261 gctgcaacga taggcaagac tgagcgtgaa actgctaaat atgcagctac gctattagct
     1321 acaaccgatg aagctaaaaa aaatcttgat tccacattaa gtaggattga cgcatcatac
     1381 gatcaaatcg aagcctatca agccgaacaa aaagccttac aagataaaaa gaccgcagat
     1441 gcaaaagctg ccagagaagc tgaagcggct gcaaaaaaat ccgctcaagc caagaagcaa
     1501 gttgtcgaac agttgcgaca aatggctaca cagtacgaga ttgctgtttt gaggcaaaag
     1561 ggaatgcatc tcgaagccgt aaaattagaa gctggtatgc gtttaggagc tgcggcaaca
     1621 aaagagcaac gagaagaagc tgaaaaacta gcacaaagta tttatggagc aaccgcagca
     1681 agtcagaact ttaacgctat acaagcccaa gcatctcctg ttactgcatt agaccaacga
     1741 catcaacaac agctagctca gatcgaagaa tataaaatct tgtatccaca aaaaataatg
     1801 gaagcagaag ctgcgagagc tgcaatcgaa gaacaatacc gccagcaacg aatggaagct
     1861 caatgggagg agtggaagca agcgagcgac ggggcgagaa tgtttggtga tgcggttgat
     1921 gctgttggtt caagtgcaac aagcacaatt acggggctac taaatggcac aatgtccgtg
     1981 cgtgatgctt tcgcatcaat tgcaaatacg atcttaaata gtgtagtaca aagccttgtc
     2041 gaaatgggta tggctcaagt taagcaaatg attatggggc aaactgctgc caaggcttct
     2101 atggcggcgc aaatggtgca agctaaagcg ttagctgctt catttgcacc cgcggcctct
     2161 atggtttctc ttgccacaca gggggcgaat gctattcccg ctcaagctgc gatcacgtca
     2221 actatgagta tggctcaagt cgccgcggtg acaggtcgta aacttggcgg ccctgtatca
     2281 gctagacaga tgtatcgtgt cggcgagaac aatcagcccg aaatcttcaa agcgagcaat
     2341 gggatgcaat atatgatccc cggtcaaagc ggtcgtgtat tcagcaacaa gcagtcgcaa
     2401 ggtcaaggtg gaggcgaaag caaaacagta gctgtcatcg taaatcaaac caatcacttt
     2461 agcagcgatg aagccagtgc aaatcaagcg gctgttgcaa aagcattgag caatcaaatc
     2521 aaggcttccg tgagattgga attgcagtca caaatgcgag ctggtggagt tctagcgagg
     2581 taatcaatgc aaacatttaa tttcaaggtt gagcaagact acactgttca aaatgagccc
     2641 agtgtcgtca tactaaaatt tgccgatggt tacgagcaaa ccgctcccaa agggttaaac
     2701 cacaatctga aaaaatacca aggcattacc gtaaaagcga acaaagcaga agcggtgaag
     2761 attcagcaat ttctcaatgc tcacggtggg tataagaaat tcaactggcg cgatagaaca
     2821 agcgatgaga cggtaacggt tcgttgcaga agctggagct ccactaaatt aggtgcagtc
     2881 attgagttta cacttaattt cgatgaggtg atttaatgcc aaaagccccc cccaaaaaaa
     2941 tggcatcaga gttgataaaa ctcgaacaag atgccctgat tgagctgtgg gagatagatc
     3001 tcactcacat ctactctagc tcaaacccaa atcagcgcgg agagatattc cgctttcaca
     3061 atggcgtgag ccaaacacaa cagaacatct ggtggcaagg caatgaatac caagcatacc
     3121 cgattgccgc cgatggtttt gagatttcgg gtcaaggacc gtcaaatcgt ccaacactca
     3181 ctgtctctaa cctgtacggt gttgtcacgg tattagtaga agatttcggg caacccgtag
     3241 aggcgaaagt cagcagtgaa gagactgtag atggcaatat ccaaatgacg gtggaactgc
     3301 tgaaaaaaat ggatcaaatc gccgatcaac gctaccgtag aaatcagtta aatgatttaa
     3361 gaacaggtgg aacattatct aaataggtga agtatgccaa ttagtatttc taaccaaatg
     3421 aaactagatc ttgccaaact tgagcaaaat gcaatgctgg acttgtacga ggttgatttg
     3481 cgtaaattgc aagacaaaaa cggcaatgct ggtagtgttt accgctttta ttctgggcta
     3541 aatgaactca aaacaagtat tgtctggcaa ggtcgaactt acgaccctta ccccatcgag
     3601 gcaacaggat ttgagcgtaa cagcagcggc ccaagcaatc gccctactct tacgctatca
     3661 aatctgtttg gcttagttac gggtattgct aatcagtttg acgaatgtat cggtgcgatt
     3721 gttcgccgtc atcaggttta cgcccaatat ttagatgcag taaactttgc tggaggtaat
     3781 gccaaagccg atcctaatca ggaaatcatc agccattttg tgattgagca actttcaagc
     3841 ctcacacgag agactgctac ttttacgctt gcgttgccga ttgaaaccga taatgccaaa
     3901 atccccagca gaattattat ggcggacacc tgcacttgga tttaccgctc ggcggaatgt
     3961 ggctacaccg gcaagca
//
LOCUS       DANLYI010000198         3944 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.200, whole genome shotgun sequence.
ACCESSION   DANLYI010000198 DANLYI010000000
VERSION     DANLYI010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3944
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.200"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            complement(<1..207)
                     /locus_tag="PXD81_002190"
     CDS             complement(<1..207)
                     /locus_tag="PXD81_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HDL5538174.1"
                     /translation="MKNSAPKKINDILQNSSLSRIVEKANFINDLNQKIQKRWPESYR
                     GLYRVVNLVDNQLIVSVKNATVRQG"
     gene            328..648
                     /locus_tag="PXD81_002191"
     CDS             328..648
                     /locus_tag="PXD81_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2547 family protein"
                     /protein_id="HDL5538175.1"
                     /translation="MSLFLKHFHRTPFWSQLFLGMVAILALPEIQVAYANESEQETVL
                     NQSFLQYAQSANDIEQQSLFIAELIHKIVEIKPQAVHFCEFFANSYRLDRQNAYPIRA
                     GPQT"
     gene            707..3433
                     /gene="secA"
                     /locus_tag="PXD81_002192"
     CDS             707..3433
                     /gene="secA"
                     /locus_tag="PXD81_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005616700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HDL5538176.1"
                     /translation="MISKLLTSIFGSSNDRTLKRLRKRVAQINKLEPTFEQLSDEALK
                     DKTAEFKQRLADGVSLDSLLPEAFATVREASKRVLGMRPFDVQLIGGMVLTGRNIAEM
                     RTGEGKTLTATLSCYLNALTGKGVHVVTVNDYLARRDAETNRPLFEFLGLTVGVNIPG
                     LPSDAKRAAYQADITYSTNSELGFDYLRDNLAHDKNERFQRELHYALVDEVDSILIDE
                     ARTPLIISGPAEDATQIYQAVDQIIPHLIAQDKEDSEEYTGEGDFTLDLKNKQAHLTE
                     RGMVKVEDFLTQMGLMEEGESLYHPARISLLHHVSAALRAHKLFERNVDYIVKDGEIV
                     IIDEHTGRTMAGRRWSDGLHQAIEAKERVAIQGENQTVASITYQNYFRLYEKLAGMTG
                     TADTEAFEFQQIYGLNTVVIPTNRPVIRDDRTDLMFKSEAEKFAAIITEIRDCIERKQ
                     PVLVGTASVEKSELLSAELTKAGIAHNVLNAKFHAQEAEIVAEAGAPGAVTIATNMAG
                     RGTDIVLGGNWKMEVAKLENPTQEQIDAIKAAWQERYDIVMQAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDPGSSRFYLSLDDALMRIYLNEGKLNMMRKAFTEEGEAMESKLLT
                     KVIASAQAKVEAHNFDGRKHLLQYDDVANEQRKVIYEQRNHLLETDNIASMIESIRQD
                     VFESTINQYIPPQSIEEMWNVPGLEERLRREFGLDLPISHWLETEKDLHEETLRERIL
                     NIAIAEYNAKEEIVGAEVMRNFEKGVMLQNLDELWKEHLASMDYLRKGIHLRGYAQKD
                     PKQEYKKESFAMFTNMLDTLKLNVISILSRIQVRSKEEVEEAQRQQAEMDAASTPVTQ
                     ATDSAAGSREENSAESLSEEELANLNIGRNDPCPCGSGKKYKHCHGNKARYS"
     gene            3520..3918
                     /gene="mutT"
                     /locus_tag="PXD81_002193"
     CDS             3520..3918
                     /gene="mutT"
                     /locus_tag="PXD81_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HDL5538177.1"
                     /translation="MSKPIIQVSAGIIRNEFGQIYLTQRLEGQDFAQSLEFPGGKVDP
                     NETPEEALKRELEEEIGIQVLSAFPYENFCFEYPTKIIEFFFYLVEEWVNEPYGREGQ
                     EGFWIAQSDLDEGAFPPANAQLIKRLKEHA"
BASE COUNT         1225 a          723 c          870 g         1126 t
ORIGIN      
        1 accttgccgt acggttgcat tcttaacgct cacaataagc tgattatcaa ctaaattcac
       61 tactcggtaa agcccacgat aagattctgg ccatcttttc tgaatttttt ggtttaaatc
      121 gttaatgaag tttgcttttt cgacaatacg gcttaagctt gagttctgta aaatgtcatt
      181 aatcttttta ggtgctgaat ttttcatctg tttctttaca tttcgtctta ttttaggctt
      241 gattttacga tacttgaccc aataatttac tcattcggct ttactttagc gtataattag
      301 cccaattttt tcatttatct taatttaatg agcttatttt taaaacactt tcatcgaacg
      361 cctttttggt cgcaactctt tttgggaatg gttgcgatac ttgctttacc tgaaattcag
      421 gttgcgtacg ccaatgaaag tgaacaggaa accgttctta atcaatcttt tcttcaatat
      481 gctcaatccg ctaatgatat tgagcaacaa tcgcttttta ttgcagagtt aatccataaa
      541 attgtggaaa ttaaaccaca agcggtgcat ttttgcgaat tttttgcaaa ctcctaccgc
      601 ttggatagac aaaatgctta ccccattcga gccgggccac aaacctaaat ataatttaat
      661 tttaccatta attgattaac ccatttttat atttaggaat aacacaatga tttcaaaatt
      721 actgacttct atttttggct caagcaacga tcgcacttta aaacgtttaa gaaagcgagt
      781 ggcacaaatt aataagcttg aaccaacctt cgagcaatta tctgatgagg cgttaaagga
      841 taaaacagcg gagtttaagc aacgccttgc agatggtgta tcgctagata gcctattacc
      901 tgaagctttt gcgacagtga gagaagcaag caaacgagta ttaggtatgc gaccatttga
      961 tgtacagctg atcggcggta tggtgctaac ggggcgtaat attgcagaaa tgcgtactgg
     1021 ggaaggtaaa accctaactg caactttatc ttgttatttg aatgctttga ccggtaaagg
     1081 tgttcatgtt gttacggtga atgattacct cgcccgccgt gatgcggaaa ccaaccgtcc
     1141 gttatttgag tttttaggct taaccgttgg agtgaatatt ccggggttac cttccgatgc
     1201 aaaacgtgct gcataccaag cagatattac ctattcgact aacagcgaat taggttttga
     1261 ttacttgcgt gataacttag ctcacgataa aaacgaacgt ttccaacgtg agttacacta
     1321 cgcattagtt gatgaagtag actctatttt aattgatgaa gctcgtacgc cgttaattat
     1381 ttcagggcct gcggaagatg cgacccaaat ttatcaagcg gtagatcaaa tcattccgca
     1441 tttgattgct caagataaag aagacagtga agagtacacc ggtgagggcg attttacctt
     1501 agatctgaaa aacaaacagg ctcatttaac cgagcgtggt atggtgaaag tagaggattt
     1561 cttaactcaa atggggttaa tggaagaggg cgaaagttta tatcaccctg ctcgtatttc
     1621 gctgttacac catgtgagtg cagcattgcg tgcccataaa ttatttgaac gcaacgtaga
     1681 ttacattgta aaagacggtg aaattgtgat tattgatgag cacaccggtc gtacaatggc
     1741 tggacgtcgt tggtctgacg gcttacacca agcgattgaa gctaaagagc gtgtggctat
     1801 tcagggcgaa aaccaaactg ttgcctcaat tacttaccaa aactatttcc gtttgtacga
     1861 aaaattagcc ggtatgactg gtacggcaga caccgaagcc tttgaattcc agcaaattta
     1921 tggcttaaat acggtggtga ttccaaccaa cagaccggtg atccgtgatg accgtacaga
     1981 cttaatgttt aaaagcgaag cggagaaatt tgcggcgatt attactgaaa ttagagactg
     2041 tatcgagcgt aaacagcctg ttttggttgg cacagcatcg gtggaaaaat cagagttatt
     2101 atcggcagaa ttaaccaaag ccggtattgc tcataacgta ttaaatgcga aattccacgc
     2161 tcaagaggct gaaattgtag cggaagcggg agctccgggg gcggttacta tcgcaaccaa
     2221 tatggctggt cgtggtacgg atattgtgct tggcggtaac tggaaaatgg aagtagccaa
     2281 gcttgagaac ccgacacaag aacaaattga tgcgattaaa gctgcgtggc aagagcgtta
     2341 tgacatcgta atgcaagccg gtggtttaca tattattggt acagaacgcc acgaatcccg
     2401 ccgtatcgac aaccagttgc gaggtcgttc tggtcgtcaa ggtgacccgg ggtcatcacg
     2461 tttctatctc tctttagatg atgccctaat gagaatttac ctcaatgaag gtaaattaaa
     2521 tatgatgcgt aaagccttta ccgaagaagg tgaagcgatg gagtctaaac tgttaaccaa
     2581 agtgattgcc tctgcacagg cgaaagtaga agcgcataac tttgatgggc gtaagcattt
     2641 attacaatat gatgatgttg ccaatgaaca gcgtaaagta atttacgaac aacgcaatca
     2701 tctattagaa acagataata ttgcctcaat gatcgaaagc attcgccaag atgtatttga
     2761 aagcacgatt aaccaatata ttccgcctca atctatcgag gaaatgtgga atgtgccggg
     2821 cttagaagag cgtttacgcc gtgaatttgg gttggattta ccgatttctc actggcttga
     2881 aactgaaaaa gacttgcacg aagagacttt gcgtgaacgt atcttaaata ttgcgattgc
     2941 agagtacaat gcgaaggaag aaatagtagg cgcagaggta atgcgtaatt ttgaaaaagg
     3001 tgtaatgcta caaaacttag atgagctttg gaaagagcat ctagcctcaa tggattattt
     3061 acgcaaaggt attcacttgc gtggttatgc acaaaaagat ccgaagcaag agtataaaaa
     3121 ggaatctttt gcgatgttta ccaatatgct tgatacctta aaattgaatg taattagcat
     3181 tttaagccgt attcaagtgc gtagcaaaga agaggtggaa gaagcgcaac gccagcaagc
     3241 tgaaatggac gctgcaagta cgccagttac acaagctacc gattctgctg ctggtagtag
     3301 agaagaaaat agcgcagaga gcttaagtga agaagagttg gcaaacttaa atattggtcg
     3361 aaatgaccct tgcccttgtg gttcgggcaa aaaatataag cattgccatg gtaacaaagc
     3421 gagatattcg taattaacaa gcggtcatat tcagtcagga atttgcaaat tttctgctta
     3481 atatgaccgc ttgaatttat agaaagagtg aaggaaacta tgtcaaaacc gattattcaa
     3541 gtctcagcag ggattattcg taacgaattt gggcaaattt acttaaccca acgtttagaa
     3601 gggcaagatt ttgctcagtc tcttgaattt cccgggggga aagtcgatcc caatgaaacc
     3661 cctgaagaag cactaaaacg agaattagaa gaagaaattg gtattcaagt gttaagcgct
     3721 tttccgtatg aaaatttttg ctttgaatat ccaacaaaaa tcattgaatt tttcttttat
     3781 ttggtggaag agtgggtaaa tgaaccttac ggaagagaag ggcaagaagg tttttggatc
     3841 gcacaatcgg atttagatga gggagctttc ccaccggcaa atgcccaatt aattaaacgc
     3901 ttaaaagaac acgcataatt tgtattgaga actgtatcta aagt
//
LOCUS       DANLYI010000199         3931 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.201, whole genome shotgun sequence.
ACCESSION   DANLYI010000199 DANLYI010000000
VERSION     DANLYI010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3931
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.201"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            270..1307
                     /gene="rsgA"
                     /locus_tag="PXD81_002194"
     CDS             270..1307
                     /gene="rsgA"
                     /locus_tag="PXD81_002194"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HDL5538178.1"
                     /translation="MSKPKLTHNQQRRIQSNHHKRINKKEIEWQDEMLGEMQQGIVVT
                     RHVKHADVETAEGQIFRCNIRRTLKNVVVGDQVSWRKGSEQLQGISGVIEAVFPRKNE
                     LTRPDYYDGIKVMASNIDQIIIVSAVLPTLSLNIIDRYLVICETANIPALIVLNKIDL
                     LNESERQAVKEQLKIYEEIGYETLCLSADTGENMQALHQYLAKGTSIFVGQSGVGKSS
                     LINQLLPEVNALTGSVSDNSGLGQHTTTASRLYHLPQGGQLIDSPGIREFGLWHLEPE
                     QITLGYREFQSVLGTCKFRDCKHKTDPGCALRQEVENGEISAIRFENYHRLIESLSET
                     KSQRHFRKEEV"
     gene            1304..1903
                     /gene="rnhB"
                     /locus_tag="PXD81_002195"
     CDS             1304..1903
                     /gene="rnhB"
                     /locus_tag="PXD81_002195"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005618717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HDL5538179.1"
                     /translation="MMNEFIYPNARLIAGVDEVGRGPLVGAVVTAAVILDPNNPIQGL
                     TDSKKLSEKKRQALAEEIKAKALSWSLGRAEPTEIDELNILNATMLAMQRAVAGLHIQ
                     PDFVLIDGNRPPKLPMPAQAVVKGDSLVAEISAASIIAKVARDQEMDELDQQFPQYGF
                     AQHKGYPTKLHFEKLAEFGATPYHRKSFAPVRKVLGLID"
     gene            1916..2275
                     /locus_tag="PXD81_002196"
     CDS             1916..2275
                     /locus_tag="PXD81_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HDL5538180.1"
                     /translation="MTTEYQKTIFTKIINKEIPANIVYQDELVTAFRDIAPQAPTHIL
                     IIPNKFIPTVNHVSNEDELALGRLFTVAAKLAQEEGIAEEGYRLVMNCNKNGGQEVFH
                     IHMHLLGGQKLGPLVAK"
     gene            2278..2649
                     /locus_tag="PXD81_002197"
     CDS             2278..2649
                     /locus_tag="PXD81_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HDL5538181.1"
                     /translation="MRISYFAFAIFSIFLTACGSKPVSYLPTGSEPIVNIESDIAEKI
                     ELETNSKQFSTTNLTEFPLNLTYKLFWYNKDGVTQSHNGISDSIGWQHLWLQPKQKQT
                     IALIKPTEESHNYRLYLRGVK"
     gene            complement(2684..3313)
                     /locus_tag="PXD81_002198"
     CDS             complement(2684..3313)
                     /locus_tag="PXD81_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012262706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDL5538182.1"
                     /translation="MFNLQIIPVSPFQQNCTVIWDENKNAAIIDGGDNAEGIIQFVES
                     QQLKVQKLLITHGHLDHIMAVEKLREHFQVEVFGSHLADKPLFERLPEMCQQFGLPPV
                     NGFLPDHWLEEGDRVEVGELSYTVRHLPGHAPGHIGFFDFDNRIAFSGDVLFQNSIGR
                     TDLYLGNHSQLLETIREKMFDLDDDFIIIPGHGPHTTIGREKQTNPFLK"
     gene            complement(3330..3884)
                     /locus_tag="PXD81_002199"
     CDS             complement(3330..3884)
                     /locus_tag="PXD81_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbK family protein"
                     /protein_id="HDL5538183.1"
                     /translation="MKQINVQRRKWLSLGGLVLGASLLPGQVMAALSTPAPAALRFRN
                     VNTGDTYTAKFGAGGLNKTDLSQLNYLMRDRHINQVKAIDPKLFVKLNQLQRRLGFHN
                     AEILVLSGYRSAQTNARLRRRSRGVASHSYHILGQAVDFQVSGVPLYKVKQAAESLNN
                     GGVGYYPRSNFVHVDTGPVRTWRG"
BASE COUNT         1223 a          839 c          786 g         1083 t
ORIGIN      
        1 tattggtcta ggaaactaga ctagagtaac gaaagttact tgtttcactt cctgtatatt
       61 ttgaaccgta ttggtttata accgcccact ttgggcggtt tttttctatg taaaaaaaca
      121 gcacaaaacc tagatcgcat tctatattat tcgctacctt tattctagcc tttctgcttt
      181 tttattcttt cactttaatc tattataaaa agaggtttta tagtatgcta aagcctattt
      241 ttcatttgca ataattaccg agagttcttt tgagtaaacc gaaattaacc cataaccaac
      301 agcgtagaat ccaatctaac catcataagc gtatcaacaa aaaagagatt gaatggcaag
      361 atgaaatgtt aggagaaatg caacagggca tcgtcgttac tcgccacgtt aaacacgctg
      421 atgttgaaac cgcagagggg caaattttcc gctgtaatat ccgccgtaca ttgaaaaatg
      481 tagtggtggg cgatcaggtt tcttggcgta aaggtagtga gcaactacaa gggattagtg
      541 gggtcattga agccgtcttc ccacgtaaaa atgaacttac ccgccccgat tattatgacg
      601 gtattaaggt gatggcatcg aacatcgatc aaatcattat tgtatcagct gtgctaccta
      661 cactttcgct gaatattatc gaccgttatt tagtgatttg cgaaacggcg aatattcctg
      721 ccttaatcgt gctaaacaaa attgatttat taaacgaaag cgaacggcaa gcagttaaag
      781 agcagctcaa aatctatgaa gagattggct atgaaaccct atgtttatcg gctgatacgg
      841 gtgaaaatat gcaggcgtta caccaatatc ttgctaaagg tacgtctatt tttgtcggac
      901 aatccggcgt ggggaaatcc agcctaatta accaactgtt acctgaagtc aatgctctca
      961 ctggctcagt gagcgataac tcagggttag gtcagcacac caccactgcc tctcgcttat
     1021 accatttacc gcaaggcgga cagctgattg actcgccggg cattcgagaa tttggcttat
     1081 ggcatttaga acctgagcaa atcaccttag gctatcgaga atttcaatcg gttttaggca
     1141 cgtgcaaatt ccgtgattgc aaacacaaaa ccgacccggg ttgtgcctta agacaagagg
     1201 tcgaaaatgg cgaaatttct gcaattcggt ttgaaaatta ccatcgctta attgaaagcc
     1261 tgtctgaaac caaaagccaa cgccatttca gaaaagaaga agtataatga atgaatttat
     1321 ttaccctaac gcccgattaa tcgccggtgt tgacgaagtc ggacgaggac ctttagtcgg
     1381 agcggttgtg accgcagccg tgattttaga tccgaataac ccgattcagg gcttaaccga
     1441 ttccaaaaaa ttatccgaga aaaaacgcca agctttggca gaagagatta aagccaaggc
     1501 tctgagctgg tcgctaggac gtgccgagcc gacagaaatt gatgagctga atattttaaa
     1561 tgcgacaatg ttggcaatgc aacgtgcggt tgccggcttg catattcagc cggattttgt
     1621 gctaattgac ggcaatcgtc cgccaaaact gccaatgccg gcacaagcgg tggtgaaagg
     1681 agacagctta gtggctgaaa tcagtgctgc ttcgattatt gccaaagtcg cccgagatca
     1741 agaaatggac gaattagacc aacaatttcc gcaatacggc ttcgcacagc acaaaggcta
     1801 cccgactaaa ctccattttg aaaaattagc agaatttggg gcaacacctt atcatcgtaa
     1861 gagttttgcc cctgtaagaa aagtattagg tttaatcgat tagaaggaaa acacaatgac
     1921 tacagagtac caaaaaacga ttttcaccaa aatcatcaat aaagaaattc cggcaaatat
     1981 tgtctatcaa gatgaacttg tgaccgcatt tcgtgatatt gctccacaag cacccacgca
     2041 tattttgatt attccgaata aatttattcc aaccgtcaat cacgtgagta atgaagatga
     2101 attagcccta gggcgtttat ttacggtggc ggcaaaatta gcccaagaag aaggcattgc
     2161 cgaagaaggc tatcgcttag tgatgaattg taataaaaac ggcgggcagg aagtgttcca
     2221 tattcatatg cacttgttag gcggacaaaa actaggacct ttagtggcaa aataactatg
     2281 cgtatttcct actttgcatt tgcaattttt tcgatttttt tgaccgcttg tggtagcaaa
     2341 cctgtcagct atttaccaac cggctcagag cctattgtca atattgagtc tgacattgct
     2401 gaaaagatag agctggaaac gaatagcaag caatttagca ccacaaattt aactgaattt
     2461 ccgcttaatc ttacttataa attattttgg tataataaag atggggtaac acaatcccat
     2521 aacggtatat cagatagtat cggctggcaa catctatggt tgcaacctaa gcaaaaacag
     2581 accattgcac tcattaaacc gacggaagaa agccataatt accgcctcta tttacgaggc
     2641 gtaaaatagc aaaaaagcag agccaactgc tctgcttctt tatttatttc aaaaacgggt
     2701 tggtctgttt ttcccgcccg atagttgtgt gtggaccatg tcccggtatg atgatgaaat
     2761 catcatctaa atcaaacatt ttttccctga tcgtttccaa taactggcta tggttgccta
     2821 aatagagatc tgttcgacct atgctatttt ggaataaaac atcgccacta aaagcaatac
     2881 gattgtcaaa atcaaaaaaa ccgatatgac ccggagcatg acccggcaaa tgtcgaacgg
     2941 tatagctcag ctcgccgact tcgacccgat caccttcctc caaccaatga tccggcaaaa
     3001 agccatttac tggtggtaat ccaaattgtt ggcacatttc cggcaaacgt tcaaataaag
     3061 gtttatctgc aagatgcgag ccgaaaacct ctacttgaaa atgctctctc aacttttcca
     3121 ccgccataat atgatctaaa tgaccgtgcg taatcaataa tttctgaacc ttaagctgct
     3181 gagattcaac aaattgaata atgccttcgg cattatcgcc gccatcaata atagccgcat
     3241 tcttattttc gtcccaaatc accgtgcaat tttgttgaaa tggactaaca ggaataattt
     3301 gcaaattaaa cataaaataa gaccgcttgt tagcctctcc aagtgcgaac tgggccagta
     3361 tctacgtgaa caaagttact acgaggataa taacctacgc caccattatt caagctctca
     3421 gccgcttgtt ttactttata taacggcaca cctgatactt ggaaatctac tgcttgccca
     3481 agaatatggt aactatggct ggcaacacca cggcttctac gacgaagtct tgcatttgtt
     3541 tgtgcggagc gatagccact taatactagg atttccgcat tatggaaacc taaacggcgt
     3601 tgtaattgat tcaatttgac aaataatttt ggatcaattg ctttaacttg attgatatga
     3661 cgatcccgca ttaaatagtt taactggctt aaatcggttt tatttaatcc acctgcacca
     3721 aatttagcag tgtaagtatc gcctgtattc acattacgaa aacgtaacgc agctggggca
     3781 ggagtagaaa gcgctgccat tacttgaccc ggcagaaggc tagcccctaa aacaagtccg
     3841 ccgagtgata gccacttacg gcgttgaaca ttaatttgtt tcattttttc ctcataggtt
     3901 agctaatgat ttatagaaaa tcactcacaa a
//
LOCUS       DANLYI010000200         3917 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.202, whole genome shotgun sequence.
ACCESSION   DANLYI010000200 DANLYI010000000
VERSION     DANLYI010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3917
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.202"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            complement(302..1396)
                     /locus_tag="PXD81_002200"
     CDS             complement(302..1396)
                     /locus_tag="PXD81_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011848300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HDL5538184.1"
                     /translation="MKKTTLAILASALIANSALAITIYDHKETGTQIEFIGSARIKWE
                     STSDKTSPVNGKATRNHVNHAVDNNGSRFGFRLTQQVGHGFYALGRVEWRMRGDSSSQ
                     HNFDHIYDHQLYAGIGHKQYGELTYGNMTNIADEVKQTDLPNTLSLSDGLLTGSSRRT
                     LQYVYKGIEGLKLGAFYGGNSQRGNNGLDLVNKRKDIWGAAAIYNYKIDDNQSLKLAT
                     GATRERSEQSIGNYERIALAFGTAYTYGKTTLGLDLERRETEDQNAIGHKRIEKEVRT
                     VLLHRLTDQWNAYTMYAYKENKLNAVGLANDSKVKRNQFMLGTEYYIVPKHLKGFVEW
                     QATRAKHYTNDVKTAKSRNYTTVVGLRAYW"
     gene            1530..2708
                     /gene="lpxB"
                     /locus_tag="PXD81_002201"
     CDS             1530..2708
                     /gene="lpxB"
                     /locus_tag="PXD81_002201"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HDL5538185.1"
                     /translation="MKQQFPVIGLVAGEVSGDILGAGLIKALKVHYPNARFIGVAGSR
                     MIEAGCQSLFDMEELSVMGLAEVVKHLPRLLKRRKQVIDEMLKLKPDVFIGIDAPDFN
                     LTVEEKLKASGIKTIHYVSPSVWAWRQKRVFKIARAANLVLAFLPFEKAFYDKFNVPC
                     RFIGHTMADAIPLEPNRTEACLALNIDESKRYMAILVGSRGSEIQFLAEPFLKAAQLL
                     KARFPDLQFLVPMVSQKRMEQFNAIKQQVAPELELNIIQGKARQAMIASECTLLASGT
                     AALEAMLCKSPMVVGYRMKPLTYWLAKKLVKTDYISLPNLLAQAPLVPELIQEACNPE
                     NLAQELSLFLADDEKNQQHKALLKQQFMQLHQSIQCNADEQAAQAVIDLLNGSTSGQI
                     "
     gene            2735..3787
                     /gene="sohB"
                     /locus_tag="PXD81_002202"
     CDS             2735..3787
                     /gene="sohB"
                     /locus_tag="PXD81_002202"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005595593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HDL5538186.1"
                     /translation="MWKEVLLNYGIFLLELLTVFGIIAVVVMLILESKKQNENGSISI
                     TNLSEKYKEQQKSLSGFFLSEEELKHQEKQEKKVAKEKAKAEKKRLKAGEECVESPSR
                     LFVLNFNGDMMAHEVNSLRREIDAVISLANPEKDEVLLKLESPGGVVHGYGLAASQLQ
                     RLRDRNIYLTAAVDKVAASGGYMMACVANKIVSAPFAVIGSVGVVAQVPNIHRLLKKH
                     DIDVDVMTAGEYKRTVTLVGENTEKGKQKFQQELEETHSLFKQFVARHRPQLDIEQVA
                     TGEHWFGQQAIELNLVDEIATSDDLLLNAIKEKEVIELKYKEKKKLAQKLGSQFEQSA
                     ENLLAKILHKSRSTLM"
BASE COUNT         1165 a          676 c          850 g         1226 t
ORIGIN      
        1 aataacccct catcactttt tgcagtttaa cctcttttgc aacagataat ttaaatttag
       61 gcgttttggc tgctcagtta ttgccattat gattatatta gttcacacta aaattttagt
      121 gttgtttctt aaagctgaag atgctaatag gtgagtaagc ggtttttacc actttgaaga
      181 taaatgcaat agcttcttgt ttgaaggatt agttgtattt aatcataaaa aatctgcacc
      241 ttaatcagtt ggttagccca acttttaggt gcagatcatt ctagtacgct tttcagtttt
      301 attaccaata tgctcgtaag cctacaacag tcgtataatt gcgagatttt gcggttttta
      361 catcattagt atagtgttta gcacgagttg cttgccattc aacaaagcct tttaagtgtt
      421 ttggaacaat gtaatattcc gttcccaaca taaattggtt tcttttaact ttgctatcat
      481 ttgccaagcc tacagcatta agtttgtttt ctttataagc atacattgtg taagcattcc
      541 attgatcggt taaacgatgg agtaacacag tgcgaacttc tttttcgata cgtttgtgac
      601 cgatagcatt ttgatcttcc gtttcacggc gttctaaatc taagccaagg gtagtttttc
      661 cgtaggtata agctgtaccg aaggcaagag caatgcgttc ataattgccg atagattgct
      721 cagaacgctc acgggttgca cctgttgcta attttaagct ttggttatca tcaattttat
      781 agttataaat tgctgctgca ccccaaatat ctttacgttt attgactaaa tctaagccat
      841 tgtttccacg ttggctattt ccgccataga atgctccgag ttttaatcct tcgatgcctt
      901 tgtaaacata ttgaagcgta cgtcgtgatg aaccggtaag taagccatca cttaagctga
      961 gtgtatttgg taagtcagtt tgttttactt catcggcaat attagtcata ttgccgtatg
     1021 ttaattcacc gtattgttta tgcccgattc ctgcatagag ttgatgatca taaatatgat
     1081 cgaagttatg ttgtgagctt gaatcgccac gcatacgcca ttccacacga cctaaagcat
     1141 agaatccgtg acccacttgc tgggttaaac ggaaaccaaa acgagagccg ttgttatcca
     1201 ctgcgtgatt aacgtggttg cgggtagctt taccatttac cggtgaggtt ttatctgagg
     1261 tgctttccca cttaatacga gcagaaccaa taaattcaat ttgtgtaccg gtttctttat
     1321 gatcgtaaat cgtaatagct agagctgagt ttgcgattaa ggctgatgct aaaatcgcta
     1381 atgttgtttt tttcataaaa atcactccat atgaatagat aaagggaggt agtgtatgtg
     1441 aatattcatt gctagacaat aatgttttgt tagtttatat gatgattggt tataatagag
     1501 tcacttacag atcttcaata atatttaata tgaaacaaca attccccgtt attggcttag
     1561 ttgctggtga ggtctctggc gatattcttg gtgccgggtt aattaaggcg ttaaaagtac
     1621 attacccgaa tgctcgattt atcggtgttg caggctctcg aatgattgag gctggctgcc
     1681 agtctttgtt tgatatggaa gagctttctg taatggggct agcagaggtg gttaaacatt
     1741 tacctcgttt acttaagcgt cgtaaacagg ttattgatga gatgcttaaa ttgaaaccag
     1801 atgtatttat cggtattgat gcaccggatt ttaatttaac cgttgaagaa aaactcaaag
     1861 cgagtgggat taaaacaatc cattatgtta gcccttcggt gtgggcttgg cgacaaaagc
     1921 gggtgtttaa aattgccaga gcagctaatt tagtgttggc atttctgcca tttgagaaag
     1981 cgttctatga taaattcaat gtgccttgtc gttttatcgg gcatacgatg gcagatgcga
     2041 tcccactcga accaaatcgt acagaggcat gtttagcgtt aaatattgat gaatccaagc
     2101 gatatatggc aattttggtg ggaagccgtg gcagtgaaat tcagtttttg gctgagcctt
     2161 ttttgaaggc tgctcagttg ttaaaagccc gttttccgga tttacagttt ttagtgccaa
     2221 tggtcagcca aaagcgaatg gagcaattta atgcaattaa acagcaagta gctcccgagt
     2281 tggaactgaa tattattcaa ggcaaggctc gtcaggctat gattgcgtca gaatgtactc
     2341 ttttggcatc gggaacagca gcactagagg caatgttgtg caaatctccg atggtagttg
     2401 gttatagaat gaaaccgctg acttattggt tagcaaaaaa attagtgaaa accgattata
     2461 tctccttacc gaatttattg gctcaagccc cattggtacc agaattgatc caagaagcat
     2521 gcaacccgga aaatttagct caagagttat ctctattttt agctgatgat gagaaaaacc
     2581 aacaacataa agcattgcta aaacagcagt ttatgcagct acatcaatca attcaatgta
     2641 atgctgatga gcaagctgcc caagcggtta tcgatctcct aaacggctcg acaagcggtc
     2701 aaatttagtt agttttttgt aaaaaggaaa atttatgtgg aaagaagtat tacttaatta
     2761 tggtattttt ttattagagt tattaactgt atttggcatt attgcagtgg tggtgatgct
     2821 gattttggaa tcgaaaaagc agaatgaaaa cggctcaatt tcaattacca atttatctga
     2881 gaaatataaa gagcagcaaa aatctctttc cggctttttt ctaagtgagg aagaattaaa
     2941 acatcaggaa aagcaggaga aaaaagtagc aaaagagaaa gctaaagcag aaaaaaaacg
     3001 cttaaaagca ggcgaagaat gtgtagaaag cccttcccgt ttatttgtat tgaattttaa
     3061 tggcgatatg atggctcacg aagtaaattc cctacgccgt gaaattgatg cggtaattag
     3121 cctcgcgaat ccggaaaaag atgaagtttt acttaaactt gaaagtccgg gcggagtggt
     3181 gcacggttat ggattggctg cttcgcagtt acaacgtttg cgtgatcgca atatttattt
     3241 aaccgcagcg gtagataaag tagctgccag cggtggttat atgatggcgt gtgtggcgaa
     3301 taaaattgtc tctgccccat ttgcggtgat tggctcagtg ggggtggtgg ctcaggtgcc
     3361 gaatatccac cgcttactga aaaaacacga tattgatgtg gatgtgatga ctgcaggtga
     3421 atataaacgc acggtgacgc tggtgggaga aaataccgaa aaagggaaac agaaattcca
     3481 gcaagaactg gaagaaacgc actctctgtt taagcaattt gtggctcgac accgcccaca
     3541 actggatatt gagcaggttg caacaggcga gcattggttt ggtcagcaag cgattgagct
     3601 aaatttggtt gatgagattg caaccagtga tgatctgctc ctcaatgcaa ttaaggaaaa
     3661 agaagtgatc gagctaaaat ataaagagaa gaaaaaactt gctcagaaat tgggttctca
     3721 attcgagcaa tcagcagaga acctgctcgc taagatttta cataaaagtc gttcaacgct
     3781 gatgtagcgt atatattatt aaagggctat cgtttatata gccccttatt ttatcagtta
     3841 gtaatagaat gaagtatttg ttcactattt aaatgacctg gaatactttt ctttaaattg
     3901 agtgaagaat ctaacaa
//
LOCUS       DANLYI010000201         3915 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.203, whole genome shotgun sequence.
ACCESSION   DANLYI010000201 DANLYI010000000
VERSION     DANLYI010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3915
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.203"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            complement(<1..866)
                     /gene="galU"
                     /locus_tag="PXD81_002203"
     CDS             complement(<1..866)
                     /gene="galU"
                     /locus_tag="PXD81_002203"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HDL5538187.1"
                     /translation="MKVIIPVAGLGTRMLPATKAIPKEMLILADKPLIQYIVNECVAA
                     GFKEIVLVTHSSKNAIENHFDTSFELETMLEKRVKRQLLDDVRSIVPKDVTLIHVRQG
                     QAKGLGHAVLCGRTVVGDEPFAVVLPDVLLGEFTANQKTENLAAMVKRFQETGYSQIM
                     VAPVPMENVSSYGVADCHGVDIPLGGAAPIAKMVEKPSIEEAPSNLAVVGRYVFSSNI
                     WNLLEKTPVGVGDEIQLTDAIDMLIHQETVEAFHMTGRSFDCGYKVGYMKAFVEYSLN
                     HDKCGKEFKAFI"
     gene            complement(1002..1187)
                     /gene="csrA"
                     /locus_tag="PXD81_002204"
     CDS             complement(1002..1187)
                     /gene="csrA"
                     /locus_tag="PXD81_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HDL5538188.1"
                     /translation="MLILTRKIGEVLLVGDDVEITVLSIRGNQVKLGVNAPKEISVHR
                     REIYERIKASVEEENAE"
     gene            complement(1277..3904)
                     /gene="alaS"
                     /locus_tag="PXD81_002205"
     CDS             complement(1277..3904)
                     /gene="alaS"
                     /locus_tag="PXD81_002205"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005622703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HDL5538189.1"
                     /translation="MKTTSDIRQSFLEFFQTKGHTIVPSSSLVPENDPTLLFTNAGMN
                     QFKDVFLGLEKRPYSRATTAQRCVRAGGKHNDLENVGYTARHHTFFEMMGNFSFGDYF
                     KQDAIKFGWEFLTSPQWLGLPKEKLYVTVYETDDEAYDIWHKEVGVPSEHIIRIGDNK
                     GAPYASDNFWAMGDTGPCGPCTEIFYDHGPEHWGGLPGSPEEDGDRYIEVWNIVFMQF
                     NRLADGTMEKLPKPSVDTGMGLERMTAVMQHVNSNYETDIFQTLIKEVATLLNVKDLD
                     NKSLRVVADHIRACSYLIADGVLPSNEGRGYVLRRIIRRAVRHGNILGAKEAFFYKLV
                     PTLATVMGQAGEVLTQKQAHIQKTLKAEEEQFARTLERGLNLLEEALAKVENNTLSGE
                     VAFKLYDTYGFPLDLTADVCRERDIKIDEDGFNAEMTAQRERAKASSSFGTDYNNVIK
                     VDSKTCFSGYTETSLNDATVIGLFSNGKSVESIQSGENAVVILDKTPFYAEMGGQVGD
                     SGQISAEICNFEVVDTQKYGQVFGHIGQLTSGSLSVGDKVNTIVDRERRHAITLNHSA
                     THLLHSALRQVLGDHVAQKGSLVSENILRFDISQPEAITKSQLEEVERIVNAKVRENI
                     QVIIEEMDIESAKAKGAMALFGEKYGEVVRVVEMSDFSIELCGGTHVKQTGDIGLFKI
                     TSEGAVAAGVRRIEAVTGENAIAWLHTLQQAVQQSAELLKADSHSFVEKIVQLQEKAK
                     RTEKELQQLKDKLAAQAGSDLAKQATQINGVNVVIQQLENVEPKALRTMVDDLKNQLG
                     SAVVVFATTAEDKVNLIVGVTKDLTDKVNAGQLVGAMAEKVGGKGGGRPDMAMAGGSE
                     PQHLLQALSLAQEWITAKL"
BASE COUNT         1101 a          898 c          667 g         1249 t
ORIGIN      
        1 ttaataaatg ctttaaattc ttttccgcat ttgtcatgat ttaaactata ttctacaaat
       61 gctttcatat agccgacttt ataaccacaa tcaaatgagc gccctgtcat atgaaaagct
      121 tcaaccgttt cttggtgaat caacatatca attgcatccg tcagctggat ttcatcacct
      181 acgcctactg gcgttttttc taataaattc caaatatttg aggaaaatac ataacgtcct
      241 actaccgcta aattagaagg cgcctcttca atacttggtt tttcaaccat tttcgcaata
      301 ggcgctgccc ccccgagagg gatatctaca ccatggcagt cagcaacccc atagctactt
      361 acattttcca ttggaaccgg cgcaaccata atttggctat atccggtttc ttggaaacgc
      421 ttcaccatcg cagctaaatt ttccgttttt tggtttgctg taaattcacc aagtaataca
      481 tctggtaata caaccgcaaa cggttcatcg ccaaccactg ttcgtccaca tagaaccgca
      541 tgacctaaac ctttggcttg cccttggcga acgtgaatta aggtaacatc ttttggtaca
      601 atagaacgta catcatctaa taattgacgt tttactcgtt tctccagcat cgtttccaat
      661 tcaaaagagg tatcaaaatg gttttcgata gcattctttg aagagtgagt aaccaataca
      721 atttctttaa aacctgcagc cacacattca tttacaatgt attgaataag aggtttatct
      781 gcaaggataa gcatttcttt tggaatagct ttggttgcgg gtaacatgcg cgttcccaat
      841 ccagcaacag gaataatgac tttcatttgg aatcctttga tgacttaaca tattagatat
      901 aactgaaata aaccgcttag acataacagg cggtcaattt tcagaaaatt ttgcattgcc
      961 tgcttcgctg ttatcatcta gcagctttac agttaaaatg cttattccgc attttcctct
     1021 tctactgagg cttttattct ttcataaatc tctcggcggt gtacagagat ttcttttggt
     1081 gcattcacac ctaatttaac ttgatttcct cggatgctga gtaccgtaat ctcaacatca
     1141 tctcctacga gtaaaacttc cccaatctta cgagttagaa taagcataat tgtctcctaa
     1201 aaagtgaaaa actctataaa tacttaaata tagagtaaag ttttaaaggc gagttcagct
     1261 cgccttgtta ttatttttaa agttttgctg taatccattc ttgagcaagt gacaatgctt
     1321 gcaataaatg ttgtggttct gagcctccgg ccattgccat atcaggacgt ccgccccctt
     1381 tacctcccac tttttcagcc attgcaccaa ctaattgacc agcattgact ttatcggtta
     1441 aatctttggt cacacctaca atcaaattga ctttatcttc cgccgtagtc gcaaatacaa
     1501 caactgcact gcctaattga ttttttaggt catcaaccat cgttctcaat gcttttggtt
     1561 caacattctc taattgttga atcactacat tcacgccatt gatttgggta gcttgtttgg
     1621 ctaaatctga acctgcttgt gctgcaagtt tatcttttag ctgttgaagc tctttttctg
     1681 tacgtttagc cttctcttgt aattgaacaa ttttctcaac gaatgaatga ctgtctgctt
     1741 ttaacaattc agcactttgt tgaacggctt gttgtaaggt atgcaaccaa gcaatcgcat
     1801 tttcaccagt gacagcctct atacggcgta cacctgctgc taccgcacct tcagaggtaa
     1861 ttttgaataa gccaatatca ccggtttgtt taacgtgtgt gccgccacaa agttcaatcg
     1921 agaaatctga catttccact acacgcacta cttcgccata tttttcacca aataacgcca
     1981 ttgcaccttt cgcttttgca gattcaatat ccatttcttc aatgattacc tgaatatttt
     2041 cacgcacttt agcattcacg atacgttcaa cttcttcaag ttgtgattta gtaattgctt
     2101 ccggctgaga aatatcaaaa cgtaaaatat tttctgacac taatgagcct ttttgagcaa
     2161 cgtggtcgcc tagcacttgg cgtaacgcag aatgcagtaa gtgggtagca ctgtggttta
     2221 atgtaatagc atgacgacgc tctctatcta cgatcgtatt aactttgtcg ccaacagaaa
     2281 gtgagccgga agttaattgc ccaatatgcc cgaatacttg accatatttt tgcgtatcta
     2341 ctacctcaaa attgcaaatt tctgcggaaa tttgaccgct atctcccact tgtccgccca
     2401 tttctgcata gaatggggtt ttatctaaaa tcactaccgc attttcaccc gattgaatag
     2461 actcaaccga ttttccatta ctaaataacc caattacagt agcatcattt aagctggttt
     2521 cagtatatcc actaaagcat gttttactgt ccactttaat cacattgtta taatctgtgc
     2581 caaagctact gctggctttc gcacgctcac gttgtgcggt catttctgca ttgaagccgt
     2641 cttcgtcaat tttaatatca cgttcacggc aaacatctgc ggttaaatct aacgggaagc
     2701 cataagtatc ataaagtttg aacgcaactt cgccagataa ggtgttattt tcaaccttcg
     2761 ctaatgcttc ttctaataaa ttcaatccac gctcaagagt acgggcaaat tgctcttctt
     2821 ctgctttgag tgtcttttga atatgcgctt gtttttgggt cagcacctca ccagcttgcc
     2881 ccattaccgt tgcaagtgtt ggcaccaatt tatagaaaaa ggcttctttt gcacccaaaa
     2941 tattaccgtg acgaaccgcc cggcggataa tacggcgtaa tacatagcct cttccttcat
     3001 ttgaaggtaa aacaccatcg gcaatcagat aggaacaagc acgaatatgg tctgccacta
     3061 cacgtaaaga tttattatct aaatctttca cattaagcaa agtagcgact tctttaatta
     3121 aagtttggaa aatatccgtt tcgtagttgg agtttacgtg ttgcattacc gcagtcatac
     3181 gttctaaccc catccctgta tctacagaag gcttaggcag tttttccatc gtaccgtctg
     3241 ctaaacgatt gaactgcata aatacaatat tccatacctc aatatagcgg tcgccatctt
     3301 cttctgggct acccggtaag ccaccccaat gttcaggccc gtgatcatag aaaatctcgg
     3361 tacaagggcc acaagggccg gtatcgccca tcgcccagaa gttatctgaa gcataaggtg
     3421 cgcctttatt atcgccaatg cgaataatat gttccgatgg tacacctact tctttatgcc
     3481 aaatatcgta ggcttcatca tcagtttcat acacagttac atataatttt tcttttggta
     3541 agccaagcca ttgtggagaa gttaaaaact cccaaccgaa tttgattgca tcttgcttga
     3601 agtaatcacc aaagctgaaa ttccccatca tttcaaaaaa agtatgatgg cgagcggtat
     3661 aacctacgtt ttctaaatca ttgtgtttac cacctgcacg tacacaacgt tgtgccgtag
     3721 tcgctctgct ataagggcgt ttttctaacc caagaaaaac atctttgaat tggttcatgc
     3781 ccgcattggt aaataataat gtcggatcat tctcgggaac aagagagctg cttggaacaa
     3841 tcgtatgtcc tttagtttgg aaaaactcta gaaaagattg tctgatatct gaagttgttt
     3901 tcattaaaaa tcact
//
LOCUS       DANLYI010000202         3821 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.204, whole genome shotgun sequence.
ACCESSION   DANLYI010000202 DANLYI010000000
VERSION     DANLYI010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3821
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.204"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            7..792
                     /locus_tag="PXD81_002206"
     CDS             7..792
                     /locus_tag="PXD81_002206"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005716895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HDL5538190.1"
                     /translation="MPLETDHDVSVWAARYIVDRINAFQPTKEKPFVLGLPTGGTPEK
                     TYKELIKLYQAGEVSFKNVVTFNMDEYVGLPKEHPESYHSFMFKHFFDHVDIPLENIN
                     ILNGMAEDVNAECERYEEKIRFYGKIHLFMGGVGVDGHIAFNEPASSLSSRTRIKTLT
                     EDTRIANSRFFNNDVSQVPKFALTVGVGTLLDAEEVMILVTGHNKALALQACVEGAVN
                     HLWTISCLQLHRRGIVVCDEPATQELKVKTVKYFKELEKNVAR"
     gene            873..2015
                     /gene="nagA"
                     /locus_tag="PXD81_002207"
     CDS             873..2015
                     /gene="nagA"
                     /locus_tag="PXD81_002207"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HDL5538191.1"
                     /translation="MNHYALTNCVIYTGEQVLYQHALVVKEDKIDSIVLEENLPLDLQ
                     KIDLKGANLSAGFIDLQLNGCGGVMFNEEITTRTLEIMQETNLRSGTTSYLPTFITSP
                     DEGVKQAVAVMREYLSKHKNQALGLHLEGPYLSVAKKGVHRPEFIREISPEMLDFLCD
                     NGDVITKITIAAENPTADHIKRFNDAGIIVSIGHSNATFAQTKQRIAEGAAFATHLHN
                     AMSPVSNGREGGVVGAVLDSDIYTGIICDGLHVEWGNIRIAKKAKGDKLVIVSDAVAA
                     AGSDIESFIFVGKKVTVKDGKCYDEFGALGGAAITMIESIRNVVQHCDISLNEAIRMS
                     TLYPAKAIGVEETLGSLEAGKIANLAIFDNQFEVKATAVNGQLQWH"
     gene            2097..2411
                     /locus_tag="PXD81_002208"
     CDS             2097..2411
                     /locus_tag="PXD81_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2322 family protein"
                     /protein_id="HDL5538192.1"
                     /translation="MQFKDYLATFPTIDHLAGLNVINSGEVIQHIPAIEGKLGSLKLY
                     NALFAKFDGKLTASAAKQGLEWFAEHVEDAKNNVGKHPNIDLLFRVIEQDLSLSLEPI
                     VK"
     gene            complement(2490..3680)
                     /locus_tag="PXD81_002209"
     CDS             complement(2490..3680)
                     /locus_tag="PXD81_002209"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010944663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HDL5538193.1"
                     /translation="MTQAIESVLAELKRGVENIYSEEDLITKLKENRPLRIKLGADPT
                     APDIHLGHTVVLNKLRQFQQFGHEVYFLIGDFTGMVGDPSGKNSTRPPLSREDVLRNA
                     ETYKEQIFKILDPQKTKIVFNSEWLGKLGTEGMIRLASNYTVARMLEREDFKNRFTNQ
                     QPIAIHEFIYPLLQGHDSVELKADVELGGTDQTFNLLVGRELQKSAGQKPQVAMTLPL
                     LVGLDGEKKMSKSLGNYIGVTEAPSEMFGKIMSISDDLMWDWYNLLSFRPLDEIAQLK
                     ADVENGKNPRDVKILLAKEIIARFHDEAAADAAEQEFINRFQKGAIPDEVPEFTFEGE
                     IGLAALLKEAGLVPSTSEAIRSAQQGGVKIDGEKVEDVKQTAQKGTFVYQVGKRKFAR
                     ITVK"
BASE COUNT         1099 a          736 c          867 g         1119 t
ORIGIN      
        1 cgtttaattc cgttagaaac tgatcacgat gtgagtgttt gggctgctcg ttatattgtt
       61 gatcgtatca atgcattcca accaaccaaa gaaaaacctt ttgtgcttgg cttaccaacc
      121 ggcggtacgc ctgaaaaaac ctataaagaa ttaattaaat tatatcaagc tggtgaagtg
      181 agctttaaaa atgtggtgac tttcaatatg gacgaatacg tcggcttacc taaggaacac
      241 cctgaaagct accacagttt tatgttcaag cacttttttg accacgttga tattccgctt
      301 gagaatatca atatcctcaa cggtatggcg gaagacgtga atgcagaatg cgaacgttat
      361 gaagaaaaaa tccgttttta tggtaaaatc catctgttta tgggcggggt cggtgtggat
      421 gggcatattg cgtttaatga gcctgcatct tcgctttctt cccgcactcg aattaaaacg
      481 cttaccgaag atactcgcat tgcaaactca cgtttcttta acaatgacgt aagccaagtg
      541 ccgaaatttg ctttaaccgt tggggttggt acgttgcttg atgccgaaga agtgatgatc
      601 ttagtgaccg gccataacaa agccttagca ttacaggcgt gtgtggaagg ggcggtaaat
      661 catctttgga ccatcagttg cttacaatta catcgccgtg gtatagtggt gtgtgatgag
      721 ccggcaaccc aagagctgaa agttaaaaca gttaagtatt tcaaagagtt agagaaaaac
      781 gtagctcgct aattctgatt ttacaagcgg ttaattttgt aatatttttt acaaaaacga
      841 ccgcctgtta gcatttaaag agaagagaga aaatgaacca ttatgcctta acaaactgtg
      901 tgatttacac cggtgaacaa gtgctttacc aacacgcatt agtggtgaaa gaagataaaa
      961 tcgactccat tgttttggaa gaaaatttac cgctggatct gcaaaaaatc gacctaaaag
     1021 gagcgaatct ttcagccggt tttattgact tgcaactgaa cggctgcggt ggggtgatgt
     1081 ttaatgaaga aatcactacc cgcactttag aaattatgca ggaaaccaat ctgcgttcag
     1141 gcacaaccag ctatttaccg acttttatta cctcgcctga tgagggagtg aaacaagcgg
     1201 tggcggtaat gcgtgagtat ttgtcgaaac acaaaaacca agcactcggc ttgcatttgg
     1261 aaggacctta tctaagtgtg gcaaagaaag gagtacaccg tccggaattt atccgtgaaa
     1321 tcagccctga aatgttagat ttcttatgcg ataacggcga tgtaattacg aaaatcacga
     1381 ttgcagcaga aaacccgacc gcagaccata tcaaacgctt taatgatgca ggaattattg
     1441 tatctatcgg gcattccaat gccacttttg ctcaaaccaa acagcgaatt gccgaagggg
     1501 cagcgtttgc tacgcattta cacaatgcaa tgtcgccggt cagcaacggg cgtgaaggcg
     1561 gtgtagtagg ggcggtgttg gatagcgata tttacaccgg cattatttgc gatggcttgc
     1621 acgttgaatg gggcaacatt cgcattgcca aaaaagccaa aggcgataaa ttagtgattg
     1681 tcagcgatgc tgttgctgcc gcaggaagtg atattgagtc gtttattttt gtcggtaaaa
     1741 aagtcacggt gaaagatggc aaatgctacg atgaattcgg agcattgggc ggtgcggcaa
     1801 tcacgatgat cgaatccatt cgcaacgtag tgcaacattg cgatattagc ctgaatgaag
     1861 caatccgaat gagtaccctt tacccggcaa aagcgattgg ggtagaggaa acactcggct
     1921 cacttgaagc cggcaaaatt gccaaccttg cgattttcga taatcaattt gaagtaaaag
     1981 cgactgctgt aaacgggcaa ttacagtggc attaattttg ctacaaattc taagcataac
     2041 agcatataat ccaaaggcag gcattgtgtc tgccttttat ttatagggag aataacatgc
     2101 aatttaaaga ttacttagcc acttttccaa ctattgatca tctagccggc ttaaacgtga
     2161 taaatagcgg tgaagtgatt caacatattc ctgcgattga aggtaaatta ggttcactaa
     2221 aactttacaa tgcactgttt gcgaaatttg atgggaaatt aaccgctagt gcagcaaaac
     2281 aagggttgga atggtttgcc gaacacgttg aagatgcgaa aaataatgtg ggtaagcacc
     2341 cgaatattga tctattattt agggtaattg agcaagattt atctttaagt ttagagccga
     2401 ttgttaaata agattgcact agtgaaaaat aacaagcggt cattatctgc aaaatttttg
     2461 caaataatga ctgcttgtgt ttaagaggct tatttcacgg taatgcgtgc aaatttgcgt
     2521 ttgccgactt ggtaaacaaa agtgcctttt tgagcagttt gtttcacatc ttccactttt
     2581 tcaccatcaa ttttaacccc gccttgttgg gctgagcgaa ttgcctctga agtggacggt
     2641 actaagccgg cttctttcag tagagccgct aggccgattt caccctcaaa ggtaaattca
     2701 ggcacttcat caggaattgc ccctttttgg aaacggttga taaactcttg ctctgctgca
     2761 tctgctgccg cttcatcgtg gaaacgagcg ataatttctt ttgccagtag aattttcaca
     2821 tcacgcgggt ttttgccatt ttcaacatcc gcttttaatt gtgcaatttc gtctaatgga
     2881 cggaatgaga gcaggttgta ccaatcccac attaaatcgt ctgaaatcga cataattttg
     2941 ccgaacattt cgcttggagc ttcagttaca ccaatgtagt tgccgagaga tttcgacatt
     3001 tttttctcgc cgtctaagcc gacaagtagt gggagtgtca ttgccacttg tggtttttga
     3061 ccggctgatt tttgtaactc acgtccaact aacaagttaa aggtttggtc tgtaccacct
     3121 aattccacgt cagctttcag ttctaccgag tcgtgacctt gtagtagcgg ataaataaat
     3181 tcgtggattg cgatcggttg ttggttcgtg aaacggtttt tgaaatcttc acgttctaac
     3241 attcgagcaa cggtgtagtt ggaggctaag cggatcatac cttccgtacc taacttgcct
     3301 aaccattcgg agttgaatac gatcttggtt ttttgtggat ctaaaatttt gaagatctgc
     3361 tctttatagg tttcggcatt gcgtaacacg tcttcacggc taagtggcgg acgggtagag
     3421 ttttttcctg atggatcgcc taccataccg gtgaaatcac cgattaagaa atagacttcg
     3481 tgtccgaatt gttggaattg acgaagtttg ttcagcacaa cagtatgacc taagtgaata
     3541 tccggtgcag ttgggtctgc ccctaattta atacgcaatg ggcggttttc ttttaatttg
     3601 gtgattaaat cttcttctga ataaatattt tccacgccac gtttaagttc ggcaaggact
     3661 gattcgatag cttgagtcat ttatcttcct tttagtcttg aatataaaaa atggatctat
     3721 tatagcggta tgaattgaaa ttgataggct ataggcgaaa aaaaacgccg tattttgtgg
     3781 gggaatacgg cgtaaagagt tggagtgatt acctattatt t
//
LOCUS       DANLYI010000203         3817 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.205, whole genome shotgun sequence.
ACCESSION   DANLYI010000203 DANLYI010000000
VERSION     DANLYI010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3817
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.205"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            86..1270
                     /locus_tag="PXD81_002210"
     CDS             86..1270
                     /locus_tag="PXD81_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005608962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HDL5538194.1"
                     /translation="MKQKKLNTTLLFITLSLISACSSLPTSGPSYSEILASNENTAET
                     QLPEVNLIKLDNITVQNLYQEQQSQRFSGFDGSMGVGGYAGTVNVGDILEISIWEAPP
                     AVLFGGTFSAEGQGNGHLTQLPQQMVNKNGTVTVPFIGNIKVAGKTPEVIQNQIVGAL
                     SRKANQPQALVRIANNLSSDVTVIRQGNSIRMPLSANNERILDAVAAVGGTSENIEDV
                     TVRLTRGNKVRSMAFETLIADPSQNITLRSGDVIALMNTPYSFTGLGAVGNNQQMRFS
                     SKGITLAEAIGKMGGLIDERSDPRGVFVFRHIPFTQLDYTSQAHWREKGYYEGMDIPT
                     VYQVNLLEPQSIFLLQRFPIQDKDIVYVSNAPLSEFQKFLRMIFSITTPITGTANTIR
                     AY"
     gene            1342..2445
                     /locus_tag="PXD81_002211"
     CDS             1342..2445
                     /locus_tag="PXD81_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis protein"
                     /protein_id="HDL5538195.1"
                     /translation="MSKKKKHFLANPLLWLTVIIPTACSGIYYSLFASDIYVSESSFV
                     VRSPNNQSSLTGVGALLQNSGFARSQDDTYSVQEYMRSRTALEQLEAEIPVKRFYSEQ
                     GDLLSKFNGLGFNDTQEAFYRYFKERLNIDVDSISGIATLRVRAFNAEEGQKINENLL
                     QQGETLINRLNERARQDTVEFAEERVKEAEKDVNNTALALSQYRTKNKIYDLPAQSSV
                     QLSLISTLKSELIRVETQLAQLKSITPDNPQVAALQMRQKSLKKEIDDQSKQLAGGNT
                     SVATQTAEYQRLVLANELAQQQLAAAMTSLQNTRGEADRQQLYLEVIGRPSKPDWALE
                     PYRIYNIISTFFIGLILFGVLSLLLASIREHKN"
     gene            2445..3242
                     /locus_tag="PXD81_002212"
     CDS             2445..3242
                     /locus_tag="PXD81_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_157140410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL5538196.1"
                     /translation="MRYGDNTTFKRSCVIQKRVIHALLMRELITRYGRKNIGFLWLFI
                     EPLLLTLVITVAWKYIKADKFSTLNIIAFMITGYPIAMMWRNASNRAQGAISANLSLL
                     YHRNVGVLDTIFTRVLLEVTGASIAQILLMTIFIAIGWIEMPNDIFYMLCAWILMAFF
                     ALGLGLIICTLAHYIEPFGKIWGTFSFFLLPLSGAFFFVHNLPLTAQNIALWFPMIHG
                     TEMFRHGYFGETVITYESISYLIVSDIILLFIGLLCVRSFSKGIEPR"
     gene            3239..>3817
                     /locus_tag="PXD81_002213"
     CDS             3239..>3817
                     /locus_tag="PXD81_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015702020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL5538197.1"
                     /translation="MISIRNVSKQYRTNNGWKTILKDINFELKKGEKVGILGRNGAGK
                     STLIRLMSGVEPPSTGTIERQMSISWPLAFSGAFQGSLTGMDNLRFVSRIYGADFDYV
                     KSFTEEFSELGEYLYEPVKNYSSGMKARLAFALSLSIEFDCYLIDEVIAVGDARFAAK
                     CKYELFEKRKDRSLILVSHSPASIKEYCDSAMV"
BASE COUNT         1227 a          645 c          739 g         1206 t
ORIGIN      
        1 aactctctgc caagaaatta ttttctatta ggggaaattt agtctagtta tttaggatga
       61 atccaaatta attataggta ctataatgaa acaaaaaaaa ttaaatacta cattattatt
      121 tataacatta tcattaattt cagcctgctc tagcctacca acgtccggcc caagctatag
      181 cgaaatctta gcaagtaatg aaaatacagc tgaaacgcag ttgcctgaag tcaatttaat
      241 caagttagat aacatcaccg tacaaaactt ataccaagag caacaatctc aacgtttttc
      301 aggatttgat ggctcaatgg gtgtgggtgg ttatgctgga acagttaatg taggcgatat
      361 attagaaata tccatttggg aagcgccacc tgccgtgcta tttggtggaa catttagtgc
      421 tgaagggcaa ggtaatgggc atttaaccca attacctcag caaatggtga acaaaaatgg
      481 aacggtaact gtgcctttta tcggtaatat taaagttgct ggaaaaacac ctgaagtaat
      541 tcagaatcaa attgtcggag cattgagccg taaagctaat caacctcaag cattagttcg
      601 tattgcgaat aacttgtcgt ctgatgttac ggttatccgc caaggaaata gcattcgtat
      661 gccattaagt gccaataatg agcgtatttt agatgcagtt gcggcagtag gtggtacaag
      721 cgaaaatatt gaagatgtaa cagttcgttt aacacgtgga aataaagtgc gttcaatggc
      781 gtttgaaaca ttaattgctg acccctcaca aaatattaca ttacgttcag gtgatgttat
      841 tgctttaatg aatactcctt atagctttac cggattagga gcagtaggta ataatcaaca
      901 aatgcgtttt tcaagtaaag gcattaccct tgctgaagct atcggtaaaa tgggaggatt
      961 aattgatgag cgttcagacc ctcgtggtgt gttcgtattc cgccatattc ctttcaccca
     1021 gttagactat actagtcaag ctcattggcg agaaaaaggc tattacgagg gaatggacat
     1081 tccaaccgtt tatcaggtaa atttattaga accacaatca atttttttat tacagcgttt
     1141 ccctattcaa gataaagata ttgtgtatgt gtcgaatgct ccactgtctg aatttcaaaa
     1201 attcttgaga atgattttct ccattacaac gccaattaca ggtacagcta atacaattag
     1261 agcatactaa ttagctcata gataaaatgg taaaaaatca ttaaaaaatg accgcttgta
     1321 acacttttcg ttgaggattt tatgtcaaag aagaaaaaac attttttagc taaccctttg
     1381 ttgtggctaa ctgtaattat tccaactgct tgttctggaa tttattattc cttatttgcc
     1441 tctgatattt atgtttcaga atctagcttt gtggttcgtt cgcccaataa tcaatcgagc
     1501 ttaacaggcg ttggtgcttt attgcaaaac tccggtttcg cccgttctca agatgatacc
     1561 tattcagttc aggaatatat gcgttcccgt accgcattag aacaacttga agcagaaatt
     1621 ccggtaaagc gtttttatag tgagcaaggt gatttactaa gcaaatttaa cggtttagga
     1681 tttaacgata cccaagaagc attttatcgt tattttaaag aacgtctgaa tattgatgtc
     1741 gattctattt caggtattgc aacgctacgc gttcgtgctt ttaatgcaga agaaggacaa
     1801 aaaattaacg agaatttatt acagcaaggg gaaacattaa ttaaccgcct taatgaaaga
     1861 gcaagacaag atacggttga gtttgcagaa gagcgtgtaa aagaggccga aaaagacgtc
     1921 aataatactg cgttagcatt aagccaatat cgaactaaaa ataaaattta tgatttaccg
     1981 gcacaatcca gcgttcagct ctcattaatt tctaccttaa aaagtgaatt aattcgggta
     2041 gaaacacaat tagcacaatt gaagtcaatt acgccggata atcctcaggt agccgcatta
     2101 caaatgcgtc aaaaaagttt gaaaaaagaa attgatgatc agtccaaaca acttgcagga
     2161 ggtaatactt ctgtagcaac gcaaactgct gaatatcaac gtttggttct tgctaacgaa
     2221 ttagctcagc aacaacttgc agcagcaatg acttcattac aaaatacacg aggtgaagct
     2281 gatcgccaac aactttattt agaggtaatt ggtaggccaa gcaaaccaga ttgggcatta
     2341 gaaccctaca gaatttataa tattatatca acttttttta tcggcttaat tctatttgga
     2401 gtattaagtt tgttactagc aagtattaga gagcataaaa actaatgcga tatggtgata
     2461 atacaacctt taaacgttcc tgtgtgattc aaaaacgggt gattcacgcc cttttaatga
     2521 gggaattaat tactcgttat gggcgtaaaa atattggctt cttatggctt tttatcgaac
     2581 cattattgtt aacgttagtg attactgttg cttggaaata tataaaagca gataagtttt
     2641 caactttaaa tattatcgct tttatgatta caggctaccc tatcgcaatg atgtggagaa
     2701 atgcctctaa tagagctcaa ggagcaatat cagcaaactt aagtttgctt taccacagaa
     2761 atgtgggagt gctagatacg atatttactc gagttttatt agaagttact ggcgcatcta
     2821 tcgcccaaat tctcttaatg actatattta tcgctattgg ttggatagaa atgcctaacg
     2881 atattttcta tatgctctgt gcttggatat taatggcatt ttttgcctta ggtttaggtt
     2941 taattatctg tactttagca cattatattg aaccttttgg taagatttgg ggaacattca
     3001 gcttctttct acttcctctt tctggtgcat tcttctttgt ccataattta ccgctaactg
     3061 ctcaaaatat tgccttatgg tttccaatga ttcatggtac agaaatgttt agacatggtt
     3121 attttggtga aacggtaatt acctatgaga gcattagtta tcttatagta agtgatatta
     3181 ttcttctatt tataggactg ctttgtgtga gaagttttag taaagggata gaaccaagat
     3241 gattagtatt agaaatgtaa gtaagcaata tcgaactaat aacggttgga aaaccatatt
     3301 aaaagatata aattttgagc tgaaaaaagg cgaaaaagtg gggattttag ggcggaatgg
     3361 tgcaggaaaa tcaaccttaa tccgtttaat gagcggggta gaacccccat caacaggcac
     3421 aatagaacgc caaatgtcta tttcttggcc cctagcgttt agcggtgctt tccaaggaag
     3481 tttaaccggt atggataacc tacggtttgt aagtagaatt tatggtgcag attttgatta
     3541 tgtaaaatca tttaccgaag aattttctga actcggggaa tacctatatg agccggtcaa
     3601 aaactattct tctggtatga aagcaagact cgcttttgct ttatctctct ctattgaatt
     3661 tgattgttat ttaattgacg aagtaattgc tgtgggcgat gcccgatttg cagcaaaatg
     3721 caagtatgag ctttttgaaa aacggaaaga ccgttcattg attttagttt ctcatagccc
     3781 agcctcaatt aaagaatatt gcgattctgc aatggtt
//
LOCUS       DANLYI010000204         3747 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.206, whole genome shotgun sequence.
ACCESSION   DANLYI010000204 DANLYI010000000
VERSION     DANLYI010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3747
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.206"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            complement(<1..1925)
                     /locus_tag="PXD81_002214"
     CDS             complement(<1..1925)
                     /locus_tag="PXD81_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(Met) cytidine acetyltransferase"
                     /protein_id="HDL5538198.1"
                     /translation="MRTIAIITSPNNADFIQQGYFYIPENHQLKAIPFSNAKTILGQE
                     YPFAIYTMQAENGVNFNLDAFAILAGTIQENGTLFLLCPQWDNLENELDFDALRWNEN
                     QAIACPNFYQHFKQLVAKFGFEVRTNLPKLPATSGQIPSKIYQLTQEQQNILQNLPLD
                     PADIHLITAPRGRGKSTLAGRLAKALPAQNKVLITARSYSALPSFWRSAEQKIPFFAP
                     DNLIEQVKSKNISPNQWLFIDEAASLPLPILHTLCAYFEKVVLTTTTQNYEGTGRGFE
                     LKFPVMLDKPFKHWTLSQPLRWSENDPLEAFVTELLLLSEEKSPLFSKEGKGEIYSLR
                     SHYVPLLKQRSNAKRLWQKQLSKNISYSNKHLQEKIAFYHLLANAHYKTTPTDLRRLF
                     DGENQLLFSQQENEQLIGGIWAMQEGGLDEKLTQAIWLGERRPQGSLVAQYLCFQGNL
                     PQACKLHSIRISRIAVLPEKQNQGVGKRLISQFILQNSVEKRPLDFISVSFGLTTPLH
                     AFWQSCGFQLVQITPNKEASSGYQSAMMIYPISEQGKLFAQQATQAFARDFALQPFFT
                     EICEELQNFEQFRPLATHNLDERDWKNLTAFAHGKRTFSAAYVSLKRLYLSDKARFSE
                     LDIFERKGQKISKMEVE"
     gene            complement(2003..2485)
                     /gene="ybeY"
                     /locus_tag="PXD81_002215"
     CDS             complement(2003..2485)
                     /gene="ybeY"
                     /locus_tag="PXD81_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HDL5538199.1"
                     /translation="MENLYIDLQIACENTENLPSEQQFYTWVQKALAVEAKTDDFPES
                     EITIRIVDEAESHELNLTYRGKDKPTNVLSFPFEAPEGIEMPLLGDLVICRQVMEKEA
                     EEQGISAESHWAHLAIHGTLHLLGYDHIEEDEAVEMESLETEIMQSLGYEDPYLSEKI
                     "
     gene            complement(2518..2973)
                     /gene="moaE"
                     /locus_tag="PXD81_002216"
     CDS             complement(2518..2973)
                     /gene="moaE"
                     /locus_tag="PXD81_002216"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005597033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HDL5538200.1"
                     /translation="MFETLIEVQEAEFDQNRIYQWLSEQHSVGAATLFIGKVREMNLG
                     DNVSGLFLEHYPAMTKKALQEIVAQARTRWDLQRVAVIHRIGQLHTGDEIVLVGVSSA
                     HRGDAYHANEFIMDYLKTKAPFWKREQTQAGERWIEGRESDQQAAKKWQ"
     gene            complement(2975..3220)
                     /gene="moaD"
                     /locus_tag="PXD81_002217"
     CDS             complement(2975..3220)
                     /gene="moaD"
                     /locus_tag="PXD81_002217"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005631889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HDL5538201.1"
                     /translation="MIKILFFAQVRELVATDRLDVEPTFTTAEALREHLSQQGKKWQL
                     ALEKGKLLVAINQTICPLETEIKDGDEVAFFPPVTGG"
     gene            complement(3213..3689)
                     /gene="moaC"
                     /locus_tag="PXD81_002218"
     CDS             complement(3213..3689)
                     /gene="moaC"
                     /locus_tag="PXD81_002218"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005597027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HDL5538202.1"
                     /translation="MTTFTHINQNGEANMVDVSMKQETVRIARAEAFVRMNAETLQMI
                     VSGNHHKGDVFATARIAGIQAAKRTWELIPLCHPLLLSKVEVQLEALPESNQVRIESL
                     CKLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMIIENVRLLSKSGGKSGDFKVD"
BASE COUNT          978 a          814 c          787 g         1168 t
ORIGIN      
        1 ttttccacct ccattttgga gattttttgc ccttttcgtt caaaaatatc cagctctgaa
       61 aagcgtgctt tatccgataa ataaagccgt tttaaactca catacgccgc tgaaaacgtc
      121 cgcttgccat gagcaaacgc ggttaaattt ttccaatcac gttcatctaa attgtgtgtg
      181 gcaagcggtc gaaattgttc gaaattttgc aattcctcac aaatttccgt gaaaaatggc
      241 tgaagggcga aatctctggc aaaggcttgg gttgcctgct gtgcaaataa ctttccttgc
      301 tccgaaatcg ggtaaatcat cattgcactt tgataaccac tgcttgcctc tttattcggt
      361 gtgatttgca caagttggaa accacaagat tgccaaaatg cgtggagtgg agtagttagc
      421 ccaaagctca cagagataaa atcaagcggt cgtttttcta ccgaattttg caaaataaac
      481 tgagaaatca accgcttgcc aacgccttga ttttgctttt caggcaatac cgcaatccga
      541 gaaatgcgaa tggaatgcaa tttgcaagcc tgcggtaaat tcccttgaaa acagagatat
      601 tgagccacta gactgccttg cggacggcgt tcccctagcc aaatggcttg ggttaatttt
      661 tcatctaaac caccctcttg cattgcccaa attccgccga taagttgctc gttttcctgt
      721 tgtgaaaaca aaagctgatt ttcgccatca aataaacggc gtaagtcagt tggagtggtt
      781 ttgtaatggg cgttggcgag taagtggtag aaagcgattt tttcctgtaa atgcttattc
      841 gaataactta tattcttcga taattgcttc tgccacaaac gctttgcgtt tgaacgttgc
      901 tttagcaacg gcacatagtg tgagcgaagc gaataaatct cccccttccc ctctttgcta
      961 aagaggggag atttttcttc ggaaagtaat agcaactcag tcacaaatgc ttcgagagga
     1021 tcattttcgc tccaccgcaa tggttgagat aacgtccaat gtttaaacgg tttatccaac
     1081 attaccggga atttcagctc aaatcctcgc cccgtgcctt cgtaattttg ggttgttgtg
     1141 gttaaaacca ctttttcaaa ataggcacaa agtgtgtgga gtatcggcaa gggtaggcta
     1201 gctgcctcat caataaagag ccattggtta ggtgaaatgt ttttactttt aacttgctcg
     1261 attaaattgt caggtgcgaa aaaagggatt ttttgctctg ccgaacgcca aaaactgggc
     1321 aatgcagaat aagaccgtgc ggtaattaat accttatttt gtgcgggtaa cgcttttgca
     1381 agtcgccctg caagggtaga tttgccccgc ccacgagggg cggtaatcaa atgaatatcg
     1441 gcaggatcaa gcggtagatt ttgcaaaata ttttgctgtt cttgcgttaa ttggtaaatt
     1501 tttgaaggaa tttgaccgct tgtagccggt aattttggca aattagttct gacttcaaaa
     1561 ccaaatttag cgactaattg tttaaaatgt tgatagaaat tcgggcaggc aatagcctga
     1621 ttttcgttcc aacgtaacgc atcgaaatct aattcatttt ccaaattatc ccactgagga
     1681 caaagcaaaa ataaagtacc gttttcttga atagtgccgg caagtattgc aaaagcatct
     1741 aagttaaaat tcaccccatt ttctgcttgc atagtataaa tggcaaaagg gtattcttgt
     1801 cctaaaatcg ttttggcatt ggaaaagggg atagctttaa gctgatgatt ttcgggaatg
     1861 taaaaatagc cttgttgaat aaagtcggca ttgttaggag atgttatgat tgcgatcgtt
     1921 ctcatataac ttgtaggaac agctcctgtg cctgtccgat tataatggag tactggaggg
     1981 cacaggaccc tcccctacaa gactatattt tttctgaaag atacggatct tcatacccta
     2041 aactttgcat aatttcggtt tcgaggcttt ccatttccac tgcttcatct tcctcaatat
     2101 ggtcgtagcc gagcagatga agtgtgccgt gaatcgccaa atgagcccaa tgggattcgg
     2161 ctgaaatacc ttgttcttcc gcctcttttt ccattacttg acgacagatg actaaatcac
     2221 ccaatagtgg catttcgatt ccttcggggg cctcaaacgg gaaagatagc acattggttg
     2281 gcttgtcttt accacgataa gtcagattta gctcgtggct ttctgcttca tcgacaatcc
     2341 gaatggtgat ctcactctca gggaaatcat cggtttttgc ctcaactgcg agggcttttt
     2401 gtacccaagt ataaaattgt tgttcacttg gtaagttttc ggtattttcg caggcgatct
     2461 gcaaatcaat atataaattt tccattcgtt accttttgca aattattgcc attttttcta
     2521 ttgccatttt ttggcggctt gttggtcact ctcacgccct tcaatccaac gttcaccggc
     2581 ttgggtttgt tctcgtttcc aaaatggggc tttggttttg agataatcca taataaactc
     2641 gttggcgtgg taggcatcac ctcgatgagc agaactcaca ccgactaata cgatttcatc
     2701 accggtatgc agttgcccaa tgcggtgaat caccgcaacg cgttgtaaat cccaacgagt
     2761 gcgagcttga gccacaattt cttgcaatgc ttttttagtc attgccggat agtgttcaag
     2821 aaacaaaccg gaaacattat cgcccaaatt catttcacgt actttaccga tgaatagagt
     2881 ggctgccccc acgctatgtt gttcactgag ccattggtaa atgcggtttt ggtcaaattc
     2941 agcttcttgt acttcaatca gggtttcaaa cattttagcc cccggttacc ggtgggaaga
     3001 atgccacttc atcgccgtct ttgatctccg tttcaagcgg gcagatcgtt tgattaattg
     3061 caaccagcag tttgcctttt tcaagggcaa gttgccattt tttaccctgt tggcttaagt
     3121 gttcacgcaa cgcttcggca gtggtaaaag taggctcaac atctagtcta tccgttgcca
     3181 caagttcacg cacttgggcg aaaaaaagta tcttaatcaa ccttgaaatc tcctgatttt
     3241 ccgccacttt tactaagtaa tcttacattt tcaataatca tatctttttg cacggcttta
     3301 cacatatcgt aaatagtcag agcagcaaca ctagctgcgg ttaatgcctc catttccacg
     3361 ccggttttgc cggttagttt acagagtgat tcaattcgca cttggttgct ttccggcaac
     3421 gcctccagtt gaacttccac tttagaaagc aataacgggt ggcagagtgg aatcagctcc
     3481 caagttctct tcgccgcctg aataccggca attcgagcgg ttgcgaatac atcgcctttg
     3541 tggtgattac cactgacgat catttgtaat gtttcggcat tcattcgcac aaatgcctcc
     3601 gctcgggcaa tgcgtacggt ttcttgtttc attgaaacat caaccatatt ggcttcgccg
     3661 ttttggttga tgtgggtgaa ggttgtcatt tgtttgtctc tttaaatttg attgtaaaat
     3721 taatattgag cgtcttcttg ttacttt
//
LOCUS       DANLYI010000205         3736 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.207, whole genome shotgun sequence.
ACCESSION   DANLYI010000205 DANLYI010000000
VERSION     DANLYI010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3736
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.207"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            9..395
                     /locus_tag="PXD81_002219"
     CDS             9..395
                     /locus_tag="PXD81_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015432850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HDL5538203.1"
                     /translation="MTVIHTENAPAAIGPYVQAVDLGNMVLTSGQIPVNPETGEIPSD
                     IVQQTRQSLNNVKAIIEQAGLTVADIVKTTVFVKDLNDFAKVNAEYQAFFQENEHPNF
                     PARSCVEVARLPKDVGIEIEAIAVRR"
     gene            427..1164
                     /gene="pgeF"
                     /locus_tag="PXD81_002220"
     CDS             427..1164
                     /gene="pgeF"
                     /locus_tag="PXD81_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005616864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HDL5538204.1"
                     /translation="MDKIIPNWNVPKHIHALTTLRTGGVSKPPFDSFNLGDHVNDEPQ
                     DIAQNRALLVEKFQLPQAPLFLTQTHSTRVIELPYSGDNIKADAIYTNQPNQVCLVMT
                     ADCLPVLFASQDGSEIAAAHAGWRGLCDGILEATVEKFNCPPHEISAWLGPAIGPNAF
                     QVGSEVADQFCAFDPRAKEALIEDSTTSGKFLGNLYQIATQRLNKLGITAISGGEYCT
                     YSQPELFFSYRRDKQTGRMATLIWRTE"
     gene            1258..1333
                     /locus_tag="PXD81_002221"
     tRNA            1258..1333
                     /locus_tag="PXD81_002221"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1291..1293,aa:Ile,seq:cat)
     gene            1536..2750
                     /locus_tag="PXD81_002222"
     CDS             1536..2750
                     /locus_tag="PXD81_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDL5538205.1"
                     /translation="MTATKKTLSDTLLKNLKYSPNAKPIADSNGLFILAQKKAKVWIY
                     SYISPKTGKRSTKNRIGSYPQMSLAEARLKRDELNTLVLQGLDPFEHLAKMQREAKEK
                     QESIQSFAYKWADWKLSKGKCKPKTMEKCLQRLENHLFPRFEGYTLEDFTLTDTINRL
                     TDLDRIMPDTLHRIAGNLIEILDYAVLCGRINFNPISVIKKAFSSVSTTHQPAIMPEE
                     LAEFMQDLQKSNRTPQIKLLVEWQLLNILRPFEAVAVQWSDIDWETKTLNIPADRMKG
                     GKNPHSVPLTKQAIAILEEMKKYNGHHKHIFTHRTDRNKPANSQTVNNAIKRLSGGKY
                     KGLLVAHGLRSIASTYLHERFTTETLVIEACLSHTNKDTVMSAYHRKNWLDRRRGIMQ
                     EWADFVEQCKSI"
     gene            3220..3426
                     /locus_tag="PXD81_002223"
     CDS             3220..3426
                     /locus_tag="PXD81_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HDL5538206.1"
                     /translation="MENTPAKQRFMKIDEVSAVTTLSETQIYRKMKAGDFPQSISIGA
                     NSKVWLESEIQQWIQRKIEQHRNQ"
     gene            3436..>3736
                     /locus_tag="PXD81_002224"
     CDS             3436..>3736
                     /locus_tag="PXD81_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5538207.1"
                     /translation="MTLETFNQQKAKFHEQEKAVLAIQTELDKAKNILEALQNEKAEF
                     VARQKTNLANIGTLSADEYVEIKNKNSGLQARIEYYQALIVDLENKCYAEQEILFQ"
BASE COUNT         1282 a          805 c          721 g          928 t
ORIGIN      
        1 aacacaagat gacagttatc cacacagaaa atgcaccggc agcgattggg ccttatgtgc
       61 aagcagttga tttaggcaat atggttttaa cttctgggca aattcccgtg aatcctgaaa
      121 ccggcgaaat cccgagtgat attgtgcaac aaacccgcca atctctgaac aacgtgaaag
      181 ccattatcga acaagccggc ttaaccgttg ccgatattgt aaagaccacc gtatttgtca
      241 aagatcttaa cgacttcgca aaggtaaatg cggaatacca agccttcttc caagaaaacg
      301 aacaccctaa ttttccggct cgttcttgcg tagaagtggc tcgtttacca aaagatgttg
      361 gcattgagat cgaagcgatt gcagtacgcc gataacaagc ggtcattttc ttgagatttt
      421 ttgcaaatgg ataaaattat tccaaattgg aatgtgccta aacatattca cgcactgacg
      481 accttaagaa caggcggagt aagcaaaccg ccttttgaca gtttcaactt aggtgatcac
      541 gttaatgacg agccacaaga tatcgctcaa aatcgtgctt tattggttga gaaatttcaa
      601 ttaccacaag ctccgctgtt tttaacccaa actcacagca cccgagtgat tgaacttccc
      661 tattcaggtg ataatattaa agccgatgcg atttacacca atcaaccaaa ccaagtctgc
      721 ttagtgatga ctgcagattg tttgccggta ttatttgcca gccaagacgg gtcggaaatt
      781 gcggcggcac acgccggctg gcgtgggtta tgcgatggca tattagaggc gacggtagag
      841 aaattcaact gcccgccaca cgaaatatca gcttggctag ggccggctat cggaccgaat
      901 gcttttcaag tcgggagtga agtggcagac caattctgtg cttttgaccc aagagcgaaa
      961 gaagccctta ttgaagacag cactacaagc ggtaaatttt taggaaatct ttaccaaatt
     1021 gccacccaac gtctgaataa attaggcatt accgccattt caggcggtga atattgcacc
     1081 tacagccaac cagaactgtt tttttcctac cgccgagaca aacaaaccgg gcgaatggca
     1141 accttaattt ggcgaacaga ataaccctat tttctctcgt tcaataatta accgagtaat
     1201 cttctgcctc tagacgacta ctcggttttt ctctataatt ccaaccactt ttcaaatccc
     1261 cccatagctc agtggttaga gcaggcgact cataatcgct tggtcgctgg ttcaagtccg
     1321 gctgggggga ccatctaagc gttgcatatt gtctttcatt gtctcaaatt tactataaaa
     1381 tcaagaactt agattctttt atagtctctt attgtcttct ttagttgcat tgtatcttag
     1441 tgtttatgat ccctaaaatg atccctattt tgaaaacggg aaaatttagg gatcataaac
     1501 ttcgataaac ccttatatat caaggattag aagaaatgac tgcaactaaa aaaacacttt
     1561 cagacacatt actaaaaaat ctcaaatatt ccccaaatgc gaagcctatc gcagacagca
     1621 acgggctatt tattcttgca cagaaaaaag cgaaagtatg gatttatagc tatatcagcc
     1681 ccaaaacagg caagcgaagc acaaaaaata gaatcggtag ctatccgcaa atgagcttag
     1741 ccgaagcacg cttaaagaga gacgaactaa atacccttgt tttacaaggc ttagaccctt
     1801 tcgagcattt agccaaaatg caaagagaag ctaaggaaaa gcaagaatct attcaatctt
     1861 tcgcctataa atgggctgat tggaagctat caaagggaaa atgcaagcct aaaacaatgg
     1921 aaaaatgctt acagcggtta gaaaatcatt tattcccacg ttttgaaggc tatacgctgg
     1981 aagattttac cttaaccgat acaatcaacc gcttaacgga cttagatcgg ataatgcccg
     2041 acactttgca cagaatagca gggaatttaa tagaaattct tgattatgcg gtattgtgtg
     2101 ggagaattaa ttttaaccca atcagcgtca ttaaaaaggc gttttccagc gtaagcacga
     2161 cacaccagcc agcaataatg cccgaagagt tagccgaatt tatgcaagac ttgcagaaat
     2221 caaatagaac gcctcaaatt aaattattag ttgaatggca actcttgaat attcttagac
     2281 cgtttgaagc cgtagcggtg caatggagcg atatagattg ggaaactaag acgctcaaca
     2341 ttccggcaga tagaatgaaa ggcggaaaaa atccgcattc tgtaccgcta acaaaacaag
     2401 ctatcgcaat cctagaggaa atgaagaaat ataacggaca ccataaacat atttttacac
     2461 accgaacaga ccgaaacaaa ccggctaata gccaaaccgt gaataatgca ataaaacgct
     2521 taagcggtgg aaaatataaa gggcttttag tggctcacgg tttaagaagc attgccagca
     2581 cctatttaca cgaacgtttt actactgaaa cgctagtaat tgaggcttgt ttatcccaca
     2641 cgaacaaaga caccgtaatg agtgcatacc accgtaaaaa ctggctagac cgcagacgtg
     2701 gaataatgca ggaatgggcg gattttgtag aacaatgcaa gagcatttaa atgacaaagg
     2761 cggagcaatc cgcctttctt tttgcttgat gaaaaatttt cattttttcc tatcagctcc
     2821 cagcccgtgc cgtctatccc acgcctcgcc cgcacactta aagtctcgat ttctacgcaa
     2881 atgcaaaatt aagcccaaac attgccccgt atagcgtgaa aaactttcat atagccgttt
     2941 tttattacgc aattttacgc aagaaaacgc aaatttacgc aggcagaaac accactttta
     3001 aaaactcgat gacagaacga tgaaagaacc gctgaaaact tgcaaaaaat cagagaaaaa
     3061 taaccgcttg caagttttcc aaatcagaat taagcccctc taatctgtaa aatctccttt
     3121 cacagcccta acaaatagca aaaaatccaa gatgatttat tctttcacac gttagcaggc
     3181 aatagcacgc caaagcaaat ttttacggag taaaacaaaa tggagaacac accagcaaaa
     3241 cagcgattta tgaaaataga tgaagtttca gccgtaacta cgctttcaga aacgcagatt
     3301 tatcgcaaaa tgaaagccgg cgattttccc caaagtatca gtattggggc aaatagcaaa
     3361 gtatggctag aaagtgaaat ccagcaatgg attcaacgca aaattgaaca acaccgcaac
     3421 caataagggc aaattatgac acttgaaaca tttaaccaac aaaaagctaa attccacgaa
     3481 caagaaaaag ccgttttagc tattcagacc gaattagaca aagcgaaaaa tattcttgag
     3541 gcattgcaaa acgaaaaagc ggaatttgtc gcacggcaaa aaacgaattt agccaatatc
     3601 ggcacgcttt cagcagatga atatgttgaa atcaaaaata aaaatagtgg tttacaagca
     3661 cgcatagaat attatcaagc gttgattgtt gatttagaaa ataaatgcta cgcagagcaa
     3721 gaaatattat tccaac
//
LOCUS       DANLYI010000206         3704 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.208, whole genome shotgun sequence.
ACCESSION   DANLYI010000206 DANLYI010000000
VERSION     DANLYI010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3704
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.208"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            139..579
                     /locus_tag="PXD81_002225"
     CDS             139..579
                     /locus_tag="PXD81_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HDL5538208.1"
                     /translation="MTACKNGLGFLVLRLFLAYEFFEAGLSKLNGENWFASIQESFPF
                     PFNVLPADLSWAIASGAEILLPILLTLGLFTRFSALGLMILTAVAWYAVHAGNGYNVC
                     NNGYKMALIYLVMLVPLLLQGAGSLSLDFLLQKKSPTKRWLKFL"
     gene            605..892
                     /locus_tag="PXD81_002226"
     CDS             605..892
                     /locus_tag="PXD81_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5538209.1"
                     /translation="MKKATLMALAGALTMATATVNAETKSVKADACMEVKDGKCVKTK
                     ATEGKCGEGKCGATSSEPKATEGKCGEGKCGATAPKAAEGKCGEGKCGASK"
     gene            993..1916
                     /locus_tag="PXD81_002227"
     CDS             993..1916
                     /locus_tag="PXD81_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 family protein"
                     /protein_id="HDL5538210.1"
                     /translation="MLKFTGAGLGYRRDLADGFLSLTDNNIINFMEVAPENWLKMGGQ
                     ARYNFDKVAEKIPVIIHGLSLSLGGQAPLDMTLLKGIKNMQQQYNSFFFSDHLSYCEC
                     EGHLYDLLPMPFTDEAVHHVANRIKQVQDYLGMQISLENTSYYLHSPTSTMNEVEFLN
                     AIAQEADCGIHLDVNNIYVNGVNHGLLDPFVFIDQVDKSRVNYIHIAGHDEDHGAAED
                     VKMTEGESFNMVKGELRHLPTLLVDTHGEAVQKTVWDLLEYTYEHLPHIPPTLLERDF
                     NFPPFQELISEVQHIMSLQKKYAKTRLKNAA"
     gene            1906..2646
                     /locus_tag="PXD81_002228"
     CDS             1906..2646
                     /locus_tag="PXD81_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding domain-containing protein"
                     /protein_id="HDL5538211.1"
                     /translation="MPLKLVEEHKPLLKEIQAEFANAIRHNDVKLMSGNISPQRLGVY
                     SRLVRNNTLGFIDRCYVQLPQHLSPEEWVAVKEKFIREGKAHSPFFQDIAGEFLNFCR
                     ENGCMPPHLLELMDFENAQLLAEVSMATVPSEFEWDNDTIMQFSGAAELRQYAVNFIR
                     SEFKQFDFEPTNVLIWRDTEFGIYYNRLEELDFFLLSSLQEGANSLNGLLAELSEFTE
                     QNLELKALLRQLWEKWVEAEVIYPVYPE"
     gene            2865..3446
                     /locus_tag="PXD81_002229"
                     /pseudo
     CDS             2865..3446
                     /locus_tag="PXD81_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252416.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
     gene            3447..3617
                     /locus_tag="PXD81_002230"
     CDS             3447..3617
                     /locus_tag="PXD81_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HDL5538212.1"
                     /translation="MLKCKQITELVSLSQEQKLTLKQRVNVQVHLMLCSRCRAFSQNC
                     KTVDKLMKEFNS"
BASE COUNT         1147 a          714 c          787 g         1056 t
ORIGIN      
        1 ctataaaaat aggttataat ttttcacaca ttttttgtaa taaacgtata cttattctgt
       61 ctaataatca catttttagt taggagcatt tttatgtcat cattttcgtc atcattttca
      121 actcagtcag cttgctcaat gacagcttgt aaaaacggct tgggctttct tgtattacgt
      181 ctatttcttg cttatgaatt ttttgaagca gggttatcaa aactaaacgg tgaaaattgg
      241 tttgcctcaa ttcaagagag cttcccattc ccctttaacg tactaccagc cgatcttagc
      301 tgggctattg ctagcggagc ggaaatctta ctgcctattt tactcacttt gggtctgttc
      361 acccgcttca gtgcgttagg attaatgatc ttaacagcgg tagcgtggta tgctgttcac
      421 gctggcaatg gctacaatgt ctgcaacaat ggctataaaa tggcactcat ttatttagtg
      481 atgttagtac cacttttatt acaaggtgct ggctcgctct ctctcgattt tctgttacag
      541 aaaaaatccc caaccaaacg ctggttgaaa ttcctttaat cccttaattt atggagaatt
      601 tttaatgaaa aaagcaactt taatggcatt agctggtgca ttaactatgg caaccgcgac
      661 tgtaaacgct gaaactaaat cagtgaaagc agatgcttgt atggaagtaa aagatggcaa
      721 atgtgtaaaa accaaagcta cagaaggtaa atgtggcgaa ggcaaatgtg gagcaacctc
      781 ttctgagcca aaagcaaccg aaggtaaatg cggcgaaggg aaatgtggag caacggcacc
      841 aaaagcggca gaaggtaaat gcggtgaagg caagtgtgga gcttctaagt aagtagcgaa
      901 ggtgcgtttt ctaaacgcac ctaacctctt ataaacacaa tctacaagcg gttaaattcc
      961 gctgattttt tgcaaattaa aggaacaaga cgatgttaaa atttactggt gccggtctag
     1021 gctatcgccg tgacctagcg gacggatttt tatctctcac agataacaat ataattaatt
     1081 ttatggaagt agccccagaa aactggctga aaatgggcgg tcaggctcgg tataacttcg
     1141 ataaagtcgc tgaaaaaatc cctgttatta ttcacggttt atccctctct ctcggcgggc
     1201 aagcgccttt agatatgacg ctactaaaag ggatcaagaa tatgcagcag cagtataact
     1261 cctttttctt ctctgatcat ttaagctatt gcgaatgtga agggcattta tatgacctgc
     1321 tgccaatgcc gtttaccgat gaagccgtac atcacgtggc aaatcgcatt aaacaagtgc
     1381 aagattattt aggtatgcaa atttcccttg aaaatacgtc ctactatctg cattcaccaa
     1441 ccagtacgat gaatgaggta gagtttctca atgcaattgc ccaagaagcg gattgtggca
     1501 ttcaccttga tgtgaataat atttatgtta atggtgtcaa tcacggcttg cttgatccat
     1561 ttgtatttat tgatcaagtg gataaatctc gtgtcaatta tattcatatt gcaggccacg
     1621 atgaagatca cggtgcggcg gaagacgtta aaatgaccga gggtgaatca tttaatatgg
     1681 taaaaggcga actacgtcat ctgccaactt tactggtcga tacacacgga gaagctgtac
     1741 aaaaaactgt gtgggattta ctagagtaca cctatgaaca cctaccgcat attccaccaa
     1801 cactgttaga acgggatttc aacttcccac cgtttcaaga actgattagt gaagtacagc
     1861 acattatgag cttacagaaa aaatatgcaa aaacgaggct aaaaaatgcc gcttaaactc
     1921 gttgaagaac acaaaccgtt actcaaagag attcaagccg agtttgcgaa tgcaattcgc
     1981 cataacgatg taaagctgat gagtggtaat atctctccac agcggcttgg cgtctattct
     2041 cgcttggttc gcaataatac tctcggtttt atcgaccgtt gttatgtaca gctccctcaa
     2101 catctaagtc ctgaagaatg ggttgccgtt aaagaaaaat ttatacgaga aggtaaggct
     2161 cattcgccct ttttccaaga tattgctggc gaatttctca atttttgccg agaaaatggc
     2221 tgtatgccac cacacttgct tgagttgatg gactttgaaa atgctcaatt actggctgag
     2281 gtatcaatgg caaccgtacc atcagaattt gaatgggata acgatactat aatgcagttt
     2341 tctggtgcag ctgagctgcg tcaatatgcg gtaaacttta tccgtagcga gttcaaacag
     2401 tttgactttg agccgaccaa cgtactgatt tggcgagata cggaatttgg catctattac
     2461 aaccgcttgg aagagctgga tttcttcctg cttagctcgt tgcaagaggg ggcaaattcc
     2521 ctgaatggct tactagcaga actcagcgag tttaccgagc aaaatctgga gctaaaagcc
     2581 ttgttacgcc aactttggga aaaatgggtt gaagcagagg tgatttatcc tgtctatcct
     2641 gaatgaggat aaggtatgtg atatgtactg acatacatag taggggcggg tcctgtgccc
     2701 gcccgactaa tattaagata acgggcgggc acaggacccg cccctacgat tagtatgtta
     2761 ggtttaaaaa tacttcatac caatatcccc taagttgggt aaaaccttat gaaataggat
     2821 acaagcggtt agtttctctc aattttttac caaaggatca cgcaatgagc caaccggctt
     2881 tacggcaaat ttccccctcg cagctagaag atattcgtct gcaaatggtg aagtttgcct
     2941 ttttacagtt aaaaaataac gaacttgccg aagatgtggt gcaagaggcg tttgtcagcg
     3001 cttataaata tgccgacagc ttcaaaggtg aaagtgcgtt aaaaacgtag atttttgcaa
     3061 ttttaaaaaa caagatcgta gatttagtga aagccaaagg caaattggtg gcagtatctg
     3121 agctgatgga agaagacgat cacgatcttg ccgataaatt gtttaacgaa aatggcggtt
     3181 gggataaaga tgcttatcaa tctaatgagt ggaagaacac cgaaagccat acctattccg
     3241 ctcaattctg gcaagtattt gaattttgcc taaataatct gccggcaaat caagcaaggg
     3301 tgtttatgat gcgttcccat ttggaaatgg aaaccgaaga aatttgcgca gaatgtgaga
     3361 tttcaccagc aaatgtgcat accttactgt atcgggcaag attgcagtta caggtctgtt
     3421 taagtcaaaa atggtttgga ggttaaatgt taaaatgtaa gcaaattacc gaattagttt
     3481 ctctgtccca agaacaaaaa ctgacattaa aacaacgtgt taatgtgcaa gttcatttaa
     3541 tgctttgttc tcgttgcaga gcctttagcc aaaactgcaa aaccgtagat aagctaatga
     3601 aagaatttaa ctcttagcgt aaaagcaagc ggtaaaattt ttaataaata tttcaaactt
     3661 aagtgagcat aaggcttatt ataaataaaa cgttccgaaa aaac
//
LOCUS       DANLYI010000207         3656 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.209, whole genome shotgun sequence.
ACCESSION   DANLYI010000207 DANLYI010000000
VERSION     DANLYI010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3656
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.209"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            74..1258
                     /locus_tag="PXD81_002231"
     CDS             74..1258
                     /locus_tag="PXD81_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005602767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HDL5538213.1"
                     /translation="MSRPLNFVMISPHFPTNFETFAIRLREAGFNTLGIADAPYESLS
                     ENLRHSLTEYYRVDNMEDYEQVYRAVAYFAHKYGRIDRIESHNEYWLELDAKLRTDFN
                     VFGYKSDDMLSIKTKAQMKEVFRKTGLKVAQGRVFKDDEDARKLAKQLKFPVIIKPNS
                     GVGASDTYKIKTAEELEAFFGYKTPTVEYIMEEFIDGDIITFDGLTDKEGNIVFYSSL
                     EYSEAVLDTVSKDGDMFYYVPCEISPKLVEMGKQCVKAFNVKERFFHFEFFRVKGSKK
                     EELLPLEINCRPPGGLTIDMWNYANDFDVFREFANIVKENKFYADIQHPWNVVYISRK
                     ANQNYVNSIEAVCEKFAGNIISVQSVPGVFAKVMGEQGILARTETMEQMREIAQFAQA
                     KQ"
     gene            1269..2021
                     /locus_tag="PXD81_002232"
     CDS             1269..2021
                     /locus_tag="PXD81_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018650716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HDL5538214.1"
                     /translation="MHFEKRSHWSGELGREMSFNVYGHAGKPVIVFPSSGGNENEYGN
                     FGMIEACRSFIERGLIKFYTPDSYDAESWLSKHKSGHDMALAHNAYDRYIIHELVPLI
                     RHESHWNGTMIATGCSMGAFHTVNFALRHPDLFDIAIALSGVYDARFFSGEFYGDKAV
                     YFNSPIDYVWGQNDDWFLDRYRQNHYVIAVGQGAWEEQHIEDTRRLQEAFNAKGIDAW
                     FDYWGFDIDHDWPTWRKQMPYFLGKLEEQGII"
     gene            2042..2494
                     /locus_tag="PXD81_002233"
     CDS             2042..2494
                     /locus_tag="PXD81_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF302 domain-containing protein"
                     /protein_id="HDL5538215.1"
                     /translation="MNLKAVAVSALAMSSVAMADIADVKPVVFESKFDFQTTVTTLTN
                     TLKEKGMTIFAEIDHQAAAKQAGLEMQPATVLVYGTPKAGTPLMVKDPMLALQLPLKV
                     LITEPNKGKVEVMLNRADQVVAHANTDYADVENNLAKAEKLIKATVAK"
     gene            2598..>3656
                     /locus_tag="PXD81_002234"
     CDS             2598..>3656
                     /locus_tag="PXD81_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL5538216.1"
                     /translation="MKLNQAIADRIVKRTMQIIPNSVNVMDSEGVIIASGDVGRIGQR
                     HTGAVIALRNDQAVEIDENLAKQWCDEVKAGINLPLHYLGSTIGVVGISGQPQQVRQY
                     AQLVKMAAELIMQQAFELEQERWQRRYREEFVRALLKGSLSTAEIQQQAVFFGQDFAK
                     PLSVMVIKIHGSTAEKLQRLIDFFEHYFPSLLTAVIGLDKIALLNHLSNEKSLIRNAL
                     NKLNDPDISFKTVVGLAVENLSQTHISYQTACHTLNYAEQIQSRKQIIHFEDFKLPAL
                     LHSFSHSWEAKTLLSPFQALLVSDKKQVLLKSLRYYFFANCDLDHAAKKLFIHPNTLR
                     YRLEKIEQITGLSFNNIEQ"
BASE COUNT         1104 a          724 c          834 g          994 t
ORIGIN      
        1 gaaaattaat aaatttagcc taaaatctat tctaatcatt ttaataatat gcaaacttta
       61 taaaggaaga actatgtcta gaccacttaa ttttgtgatg atttccccgc acttcccaac
      121 gaactttgaa acctttgcca ttcgtttgcg tgaagccggt tttaatacat tgggaattgc
      181 agatgcccct tatgaatcat tgagcgaaaa tctgcgtcat tccctgactg aatattatcg
      241 tgtagataat atggaagatt acgaacaagt ttatcgtgcg gtcgcgtact ttgcccataa
      301 atacgggcga attgaccgca ttgaatcgca caatgaatat tggctggagt tagatgcgaa
      361 attgcgtacc gatttcaatg tgtttggtta taagagcgat gatatgctct caattaaaac
      421 caaagcccaa atgaaagagg tattccgcaa aaccggcttg aaagtggcac aagggcgagt
      481 atttaaagat gatgaagatg cccgcaaatt agcaaaacag cttaaattcc cggtgattat
      541 caagccaaat tcaggggttg gggcaagcga tacttacaaa atcaaaactg cggaagagtt
      601 agaagctttc ttcggttata aaacgccaac ggttgaatat attatggaag agtttattga
      661 cggcgatatt attacctttg acggtttaac cgataaagag ggcaatatcg ttttctactc
      721 cagccttgaa tattcagaag cggtattaga taccgtttca aaagatggcg atatgttcta
      781 ctatgtccca tgtgaaattt cgccaaaatt agtggaaatg ggcaaacaat gtgtgaaagc
      841 ctttaacgta aaagagcgtt ttttccattt tgagttcttc cgtgttaaag ggagcaaaaa
      901 agaagagttg ttaccactcg aaatcaactg ccgtccgccg ggtggcttaa ctattgatat
      961 gtggaactac gccaacgatt tcgatgtgtt ccgtgaattt gccaatattg tgaaagaaaa
     1021 caaattctat gccgatattc agcacccttg gaacgtggtt tatatttccc gcaaggcaaa
     1081 ccaaaattat gtgaattcga ttgaagctgt ttgtgagaaa tttgccggca atattatcag
     1141 cgtgcaaagc gtaccgggcg tatttgccaa agtgatgggc gaacaaggca ttttagcccg
     1201 taccgaaaca atggagcaaa tgcgtgaaat tgcacaattt gcacaagcta aacagtaaga
     1261 ggtgaagtat gcattttgaa aaaagaagcc actggagtgg agaattaggt cgtgaaatgt
     1321 cgttcaacgt ttacggacac gcaggtaaac cggttattgt tttcccttcc tccggcggca
     1381 atgaaaacga gtatggtaat tttggaatga ttgaggcttg ccgttccttt attgaacgtg
     1441 gcttaatcaa attttacacg cctgattctt acgatgcgga atcttggtta tctaaacata
     1501 aatccggaca cgatatggca ttagctcata atgcttacga ccgttatatt attcacgagc
     1561 tagtgccgtt aatccgccat gaatcgcact ggaacggcac gatgattgcc accggttgta
     1621 gtatgggggc tttccatacg gtaaactttg ccttacgtca tccggatctg tttgatattg
     1681 ccatcgcctt aagcggtgtt tatgacgctc gctttttcag tggtgagttt tatggcgata
     1741 aagcggttta ttttaactcg ccgattgatt atgtatgggg tcaaaatgac gattggttct
     1801 tagaccgcta ccgccaaaat cactatgtga ttgcagtcgg acaaggtgcg tgggaagaac
     1861 agcatattga agacacccgc cgattacaag aggcttttaa cgcaaaaggg atcgatgctt
     1921 ggtttgacta ctggggcttt gatattgacc atgactggcc gacttggcgt aaacagatgc
     1981 cttatttctt aggcaagttg gaagaacaag ggattattta atctatttga ggagaaaaga
     2041 aatgaactta aaagcagtcg cagtttctgc attagcaatg agcagcgtgg caatggcaga
     2101 tattgccgat gtcaaaccgg tggtatttga aagtaaattt gatttccaaa ctacagtcac
     2161 aactttaacc aacaccttaa aagaaaaagg aatgacgatt tttgccgaaa tcgatcacca
     2221 agcggcggca aaacaagccg gcttagaaat gcaaccggca accgtattgg tttacggcac
     2281 accgaaagcc ggtacgccat taatggtgaa agatccgatg ttagcgttgc aattaccgtt
     2341 aaaagtgtta attaccgaac cgaacaaggg caaggtcgaa gtaatgttaa atcgtgccga
     2401 tcaagtggtg gctcacgcta ataccgatta tgctgatgta gaaaacaacc ttgccaaagc
     2461 ggaaaaatta attaaagcga ctgtggcaaa ataacttcac gctatgatct attctcagga
     2521 agtggaagcg tagataattt gcaaatccct cgaaagaggg atttttcata gtattgggaa
     2581 tgagtaggaa tcatataatg aaactcaacc aagcgattgc cgaccgcatc gtcaagcgca
     2641 cgatgcagat tattcccaac tccgtcaatg tgatggatag tgaaggggtg attattgctt
     2701 ccggcgatgt cggtcgtatc gggcaacgac acacaggagc ggtgattgcg ttacgcaacg
     2761 atcaagcggt cgaaattgat gaaaatcttg caaaacagtg gtgcgatgaa gtgaaagcgg
     2821 gcattaactt accgcttcac tatttaggct caaccattgg tgtagtgggg atttcagggc
     2881 agccacaaca agttcggcaa tatgctcaat tggtgaaaat ggcggcggag ctgattatgc
     2941 agcaagcgtt tgagttggaa caggaacgtt ggcaacgccg ctatcgggaa gagtttgtgc
     3001 gggccttgct caaaggtagc ctaagcacag cagaaatcca gcaacaagcg gtctttttcg
     3061 ggcaagattt tgcaaaaccg ctgtcggtga tggtgattaa aattcacggc tctactgcgg
     3121 aaaaactcca acgcctgatt gattttttcg aacattattt cccgagccta ctcaccgccg
     3181 tgattgggtt agataaaatt gccttgctga atcatttatc caacgaaaaa tcgttaatcc
     3241 gcaatgcact aaacaaactt aacgatccgg atatttcctt caaaacggtg gttgggctgg
     3301 cggtcgaaaa tttatcgcaa acccatattt cctaccaaac cgcctgccat accctcaatt
     3361 atgccgagca gatccaaagc cgtaagcaga ttattcattt tgaagatttt aaactgccgg
     3421 ccctcttgca cagtttcagc cactcttggg aagcgaaaac cttgctctcg ccgttccaag
     3481 ctttactggt ttccgataaa aaacaggtgt tactgaaaag tctgcgttac tattttttcg
     3541 caaattgtga tctggatcac gctgccaaaa aattgtttat tcacccaaat acgctacgtt
     3601 accgtttaga gaagattgag cagataactg gtttatcttt caataacata gagcaa
//
LOCUS       DANLYI010000208         3640 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.210, whole genome shotgun sequence.
ACCESSION   DANLYI010000208 DANLYI010000000
VERSION     DANLYI010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3640
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.210"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            125..487
                     /locus_tag="PXD81_002235"
     CDS             125..487
                     /locus_tag="PXD81_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HDL5538217.1"
                     /translation="MKPTNKADFQRVINAAGYSMKGLKAAYINEAAFRQEIWCATILF
                     PLGLILGETNIEKALLVGTVLLVLVTELLNSAVESVVDRIGADFHELSGRAKDIGSAA
                     VFMAMVLLAITWILILFC"
     gene            524..2167
                     /locus_tag="PXD81_002236"
     CDS             524..2167
                     /locus_tag="PXD81_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HDL5538218.1"
                     /translation="MRLKKTLLSVALLSATSAMAYETGKTYRFTVLHTNDIHGHFWQS
                     DKGEYGLAAQKTLVDNIRKEVAEKGGSVLMLNAGDANTGVPESDMQNARPDIEGMNAI
                     GYDAMVLGNHEFDYPLQILSMQEKWAKFPFISANVINKKTDKPIVKPYVMLERGGLKI
                     AVIGLTTEDTAKLGNPDVTENAFFKNPIETAKNTLAEINQKEKPDVRIALTHMGYYFD
                     GKHGTNAPGDVTMARTLEKGAFDLIIGGHTHDTVCIDEKGQFKAKYTPGEECKPDFQN
                     GTWIVQAGEWGKFVGRADFEFKDGKATLVKYELIPVNLKKTIKKEDGTKEYRLYQPEI
                     KADEATYTLLKKYQDEGDKKLNIEVGRVKGLLDGKREHIRFQQTNLGRLIAQSQMERV
                     KADIGIMNSGGIRTSLEEGVITYKHILTVQPFGNMIATVDLKGQELLDTLTTIALKEV
                     DTGAYPQFAGISMVVDRTAKTISDVKIGGKPFDKEKTYKVSLPDYVAAGGDGYPLLKK
                     HPSYVNTGFIDAEMLKKFFEENKIIEADKYDPKNQIIFK"
     gene            complement(2374..3243)
                     /locus_tag="PXD81_002237"
     CDS             complement(2374..3243)
                     /locus_tag="PXD81_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDL5538219.1"
                     /translation="MSSRGKILNTISSLQNSLTKTEKKIAAAILSQPELLSQCSLSEV
                     AKQLDVGEATFIRFCRTLGFKGYTDFKLELAIELATQNQDNRVLLDTDVSESDTSKDI
                     AEKLKVSLDNVIEETINLLDFNVLEKVVEELRKAKRIFLFGVGSSGLTAEDAKHKLMR
                     IGLQTDAVTNNHFMYMQAALVKEGDLVIGISHSGYSEEIVKSLRFSRANKATTVAITH
                     NLRSPVTEEANYVLINGNRQGHMQGDSIGTKMSQLFVLDLIYTLLVKAEPENALKQKQ
                     KTLNVILEQRVKG"
     gene            complement(3253..>3640)
                     /locus_tag="PXD81_002238"
     CDS             complement(3253..>3640)
                     /locus_tag="PXD81_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HDL5538220.1"
                     /translation="KSLDLVNLELGWQELENGIRMNVMSFETTPAEGKKAELHRKFID
                     IQLLIDGEEMIEYGLSQPDLSKYDEYRDEDDYQLTAEIDDKNNLILKPNMFAIFLPYE
                     PHKPGNSVNGNKTLKKLVVKVPVELI"
BASE COUNT         1161 a          702 c          648 g         1129 t
ORIGIN      
        1 tttacaaaaa agtgaagttt tttacattcc atggctatca tattattttt tccttaggtt
       61 ctttgaacat caccattctt tcagtacaat agttctctaa actcttttat ttattttatt
      121 tactatgaaa ccaaccaata aagcagattt tcaacgtgtg atcaatgcag ctggctactc
      181 tatgaagggg ttaaaagccg cttatattaa tgaagccgcc tttcgccaag aaatttggtg
      241 cgctactatt ttattccctc tagggcttat tctaggcgaa accaatattg aaaaagcatt
      301 attagtaggc acagttttac ttgttttagt tacagaacta ctcaatagtg cggttgaatc
      361 tgttgttgac cgcattgggg ctgatttcca cgagctttct ggtagagcta aagatattgg
      421 ctctgcagct gtatttatgg cgatggtttt actcgctata acttggatat taattttgtt
      481 ttgttaactc tatttttcaa ccaacactct tactgaggta tttatgcgct taaaaaaaac
      541 attactttcg gtagcactac ttagtgcaac atcagcaatg gcctacgaga caggtaaaac
      601 atatcgtttt acggttttac atactaacga tatacacggt cacttttggc aaagtgataa
      661 aggcgaatac ggtctcgctg ctcaaaaaac attggttgat aatattcgca aagaggtcgc
      721 agaaaaaggt ggttcggtat taatgctgaa tgcaggtgat gcgaatacag gcgtgccgga
      781 gtcagatatg caaaatgcac gtcctgatat tgaaggaatg aatgcgattg gttacgatgc
      841 aatggttcta ggtaatcacg aatttgatta cccactgcaa attctctcta tgcaagaaaa
      901 atgggctaaa ttccccttta tttcggccaa tgtcattaat aaaaaaacgg ataaacccat
      961 tgtaaaacct tacgtgatgt tagaacgagg cggactaaaa attgctgtta tcggattaac
     1021 aaccgaagat accgcaaaat taggtaatcc agatgtaacg gaaaatgcct ttttcaaaaa
     1081 ccctattgaa acagcaaaaa acacattagc tgaaattaat caaaaagaaa aacccgacgt
     1141 acgtatcgct ttaacccata tgggttacta ttttgacggt aaacatggta ctaatgcacc
     1201 aggtgatgta acaatggccc gtaccttaga aaaaggagcg ttcgatttaa tcattggcgg
     1261 tcatacccac gatactgttt gtattgatga aaaaggtcaa ttcaaagcca aatatacgcc
     1321 gggtgaggaa tgtaaaccag acttccaaaa cggtacttgg attgtgcaag ccggcgaatg
     1381 gggcaaattt gttggacggg ctgattttga gtttaaagat ggaaaagcta ccttggtaaa
     1441 atatgaatta attccggtca acttaaaaaa aaccattaaa aaagaagacg gtactaaaga
     1501 ataccgactt taccagcctg aaatcaaagc cgatgaagca acctatacct tattgaaaaa
     1561 atatcaagat gagggcgata aaaagcttaa tattgaagtt ggtcgtgtga aagggttatt
     1621 agacggcaaa cgtgagcata ttcgcttcca acaaaccaat ctcggtcgtt taattgcaca
     1681 atcgcaaatg gaaagggtaa aagccgatat cgggattatg aactccggcg gtattcgtac
     1741 ctcccttgaa gaaggtgtaa taacttataa acatattctg actgttcaac ctttcggtaa
     1801 tatgattgca acggtggatt taaaaggaca agaattgtta gataccctca ccactattgc
     1861 tctgaaagaa gttgatactg gcgcttatcc tcaatttgcc ggcatttcaa tggtagtcga
     1921 ccgtacagca aaaactattt ctgatgttaa aatcggtggc aaaccttttg ataaagagaa
     1981 aacttataaa gtttctctgc ctgactatgt tgcggctggt ggcgatggct acccattact
     2041 gaaaaaacac cctagctatg tcaatacagg ctttattgat gccgaaatgc tgaaaaaatt
     2101 cttcgaagaa aataaaatta ttgaggcaga taaatacgat ccaaaaaacc agatcatttt
     2161 caaatagagc tatagaaaat gaaactcact ctggcagtct ctgctcaaaa gagctgtggc
     2221 tgcctgtaga ttgttttctt actttcaagt gttataggaa atcgataaaa aatccctacc
     2281 aaattaattc atcaccatat gatgaaaatt aaaaaacctc tggcaatact ttgccaaaag
     2341 tctttgactt ttatagggta atatcactgc ttattagcct ttaacacgtt gttccaaaat
     2401 cacattcagt gttttttgtt tctgtttcaa cgcattttcg ggttcggctt taactagtaa
     2461 ggtataaatt aaatcaagaa cgaaaagttg tgacatttta gtaccgatag aatctccttg
     2521 catatgcccc tgtcgattac cattaattaa cacataatta gcctcttccg tgacggggga
     2581 acgtaaattg tgtgtaatcg caaccgttgt cgctttattt gcacgagaaa aacgtagtga
     2641 tttcacaatt tcttcagaat agcctgaatg actaatccct ataactaaat ccccctcttt
     2701 caccaatgcc gcctgcatat acataaaatg attatttgtg actgcatcgg tttgtagccc
     2761 aatacgcatt aatttatgtt ttgcatcttc tgcggttaac ccagaagaac ccactccaaa
     2821 taaaaagatc cgttttgcct tacgtaattc ttcaacaact ttttccaaca cgttaaagtc
     2881 aagcaaatta atagtctctt caataacgtt atctaaactc actttgagct tctcagcaat
     2941 atctttcgat gtatccgatt ctgaaacatc cgtatcaagc aatactctat tatcttgatt
     3001 ttgcgtagcc aactctattg caagctctaa tttaaagtcg gtataacctt taaaacctaa
     3061 tgtacgacaa aaacgaataa aagttgcttc accaacatct aattgtttgg ccacttcaga
     3121 taatgaacat tggctaagta attccggctg agataaaata gcagcagcta ttttcttctc
     3181 tgttttggtt aaactatttt gtagcgagct aattgtatta agaatttttc cccgactaga
     3241 catagtagcc tcctaaatca attcaacagg tactttcacc accagttttt tcaatgtttt
     3301 gttgccattc accgaatttc caggcttatg tggttcataa gggaggaaaa tagcgaacat
     3361 atttggcttg aggatcaagt tatttttatc atcaatctca gctgttaatt ggtagtcatc
     3421 ttcatctcga tattcatcat acttagataa atctggctgg cttaacccgt attcaatcat
     3481 ttcttcgccg tctatcaata attgaatatc gataaatttt cggtgtaact ctgctttttt
     3541 cccctcagct ggcgtagttt caaatgacat tacattcatg cgaataccat tttctaattc
     3601 ttgccagcct aattccaaat taactaaatc taaacttttt
//
LOCUS       DANLYI010000209         3592 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.211, whole genome shotgun sequence.
ACCESSION   DANLYI010000209 DANLYI010000000
VERSION     DANLYI010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3592
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.211"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            complement(<1..476)
                     /locus_tag="PXD81_002239"
     CDS             complement(<1..476)
                     /locus_tag="PXD81_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL5538221.1"
                     /translation="MIITMKDIAKEANVSLGTVSNVLNGKSNVSLAKIEKVQAAVEKL
                     GYRKNIQASTLKSGTSNKIAIILPNITELKYACLYENLDLICTQNDLSLILYLTHNRE
                     DREKQIIQNIKQDNYLRIILDSCLNNADYYYDEVKDQDKFIFVYRYLKNAKHFIGF"
     gene            complement(638..1978)
                     /gene="gdhA"
                     /locus_tag="PXD81_002240"
     CDS             complement(638..1978)
                     /gene="gdhA"
                     /locus_tag="PXD81_002240"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005616859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HDL5538222.1"
                     /translation="MSDLNALFQKIKQRDPNQAPFHQAVEEVFGSLAPFLAKNPKYTQ
                     QGLLGRIVEPERVISFRVTWVDDKGQVQVNRGYRVQMNSAIGPYKGGIRFHPTVDLGV
                     LKFLAFEQVFKNALTTLPMGGGKGGSDFDPKGKSDAEVMRFCQAFMSELYRHIGADTD
                     VPAGDIGVGGREIGYMFGQYKKLRNEFTSVLTGKSLTWGGSLIRPEATGYGAVYFAES
                     MLATKGQQIEGKSVVISGSGNVAQYAAEKVIQKGGKVLTVSDSNGYVLFPESGMTEAQ
                     LQALLELKNERRERLSVYATEQGLKYFDGQKPWGVKCDIALPCATQNELDTEDAKELV
                     KNGCICVAEGANMPTTLGGVEVFIGAKILYAPGKAANAGGVATSGLEMSQNAIRLSWS
                     REEVDQRLFNIMKAIHENCVENGTEADGFVNYVNGANIAGFKKVANAMLEQGIV"
     gene            complement(2171..2512)
                     /gene="bamE"
                     /locus_tag="PXD81_002241"
     CDS             complement(2171..2512)
                     /gene="bamE"
                     /locus_tag="PXD81_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HDL5538223.1"
                     /translation="MKMKSLLAAMLLALGVTACSTVEKVVYRIDVPQGNYLEQDKIAQ
                     LKVGMTAEQVEYLLGTPMLKNIFETHRWDYVFIKREGYKDPEQRNLFIYFDAKGLVSK
                     IDDSNANAEQK"
     gene            complement(2579..3580)
                     /gene="yejK"
                     /locus_tag="PXD81_002242"
     CDS             complement(2579..3580)
                     /gene="yejK"
                     /locus_tag="PXD81_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HDL5538224.1"
                     /translation="MSINVSEIVLHQLQKTEGENPELNTFLRENLLAISPEVDQMMLQ
                     LHQAYQSKAKAYGIFKPESVFAQQLNRLLEQETDFLPFSHSCAKMLAAELIKYPFADG
                     GTFILCRYTFLATEYLFIALIDSRNSMLVDEQLEIKRTQYLEIAQYDIACRINLTELK
                     LDATSNRYLTFVKGRVGRKIADFFMDFLSAEEGFNPQLQNQTLLQAVSDYCEQGELSA
                     PQTREVKKQVFEYCKGQINSGEEIALNELSNALPTLNEIDFAQFTQEQEYGLEENIPP
                     MRNALKTLTKFSGSGKGVTISFNAELLGERLIWDELNDTLTIKGLPANLRDQLERNK"
BASE COUNT         1070 a          762 c          593 g         1167 t
ORIGIN      
        1 tcaaaaccaa taaaatgttt tgcattttta aggtaacgat aaacaaaaat aaatttatct
       61 tggtctttta cttcatcgta ataatagtct gcattattta ggcaactatc taaaataata
      121 cgcaaatagt tatcttgctt tatattttga ataatttgtt tttctctatc ttctcgatta
      181 tgcgttaaat aaagaatgag agataaatca ttttgcgtac aaattaaatc aagattttca
      241 tataaacagg catatttcag ctctgtaata ttgggcaata taatagcaat tttattactt
      301 gtgccacttt ttaacgtaga ggcttgaata tttttacgat aacccaactt ttcaacggca
      361 gcttgtactt tttctatttt agctaagcta acattacttt ttccgtttaa tacattcgat
      421 actgttccta acgaaacatt tgcttccttt gcgatatctt tcatcgtaat gatcatatac
      481 tgccctttca catactgcta aaactgagat tggcatagat tttaccataa ttggcaaatg
      541 aaatacgatt tcaatttagc ttgtctctag ctacaaccta aaataaaaaa actgcctcga
      601 tatatcgagg cagttcttga ataataggga taataaatta aacaataccc tgttctaaca
      661 tcgcatttgc cacttttttg aaaccagcaa tattcgcacc gtttacatag tttacgaaac
      721 catctgcttc agtaccattt tctacgcagt tttcgtgaat tgctttcata atgttgaata
      781 aacgctgatc tacttcttca cgactccaag atagacgaat tgcattttgg ctcatttcta
      841 aacctgaggt tgccacacca cctgcgttag ccgctttacc cggagcataa aggattttcg
      901 caccgataaa gacttcaaca ccacctaatg tggttggcat atttgcacct tctgccacac
      961 aaatacagcc gtttttcact aattctttag catcttcggt atctaattcg ttttgggttg
     1021 cacaaggtaa agcgatgtca catttcacac cccacggttt ttgaccgtca aagtatttta
     1081 agccttgttc tgttgcatac acagataagc gttcacggcg ttcgtttttc aactctaata
     1141 atgcttgtaa ttgtgcctct gtcataccgg attcagggaa taagacataa ccgttagaat
     1201 ctgaaaccgt taatacttta ccgccttttt gaatcacttt ttcagctgca tattgtgcca
     1261 cgttacccga accggaaatt accactgatt ttccttcgat ctgctgacct ttagtcgcta
     1321 acatagactc tgcaaaatac accgcaccat aaccagttgc ttccggacga attaagctac
     1381 caccccaagt cagcgattta ccggttaata ctgaagtaaa ttcattgcgt aattttttgt
     1441 attgaccgaa catatagccg atttcacgac ctcccacgcc gatatcgcct gcaggaacgt
     1501 cagtatcagc accaatgtgg cgatataatt cgctcataaa cgcttggcag aaacgcatta
     1561 cttcggcatc agatttgcct ttcgggtcaa agtctgaacc acctttacca ccgcccattg
     1621 gcaatgtcgt taacgcattt ttgaatactt gctcaaaggc taagaatttt aatacgccta
     1681 aatcaacggt tgggtggaaa cgaataccgc ctttgtatgg accgattgct gagttcattt
     1741 gtacacggta accacggttt acttgcactt gacctttatc atcaacccaa gtgacacgga
     1801 agctaattac acgctcaggc tcaacaatac gccctaataa accttgctgg gtatatttcg
     1861 gatttttagc taagaaaggg gcaaggctac cgaacacttc ttcaaccgct tgatggaaag
     1921 gggcttggtt cggatcacgt tgtttgattt tttggaaaag ggcgtttaaa tcgctcatat
     1981 tatactcctg aataaattga tgatgttgtg tatttaagat gagcgaagtc tagcaagcaa
     2041 ttttagcttt gaaaagatag attagaaaaa tttatcatta aaataagaaa aattaagttt
     2101 aaatgcttat tttttattca aaaaaataag cccactttta aaagtgggct tttcgaacca
     2161 acaaatcgtc ctatttttgt tctgcattcg cattgctgtc atcaattttt gatactaacc
     2221 ctttagcatc aaaataaata aacagattgc gttgttcagg atctttataa ccttcacgtt
     2281 taataaacac ataatcccaa cggtgggttt caaaaatgtt ctttaacatt ggtgtaccaa
     2341 gcaaatattc aacttgttcc gctgtcatac cgactttaag ttgagcaatt ttatcttgtt
     2401 ctaagtaatt gccttgcggt acatcaatac ggtacaccac tttctcaacg gttgaacaag
     2461 ccgttacacc cagtgcaagc aacattgcag ctaaaagaga tttcattttc ataattgacc
     2521 ttttgataat ccaaaattca gataacgaga taatacctaa actgataaaa attttccatt
     2581 atttgttacg ttccagctgg tcacgcagat ttgccggtaa tcctttgatt gtcagtgtgt
     2641 cgtttagttc atcccaaatt aaacgctctc ctaataattc tgcgttaaag ctaatcgtta
     2701 ctcctttgcc cgaaccggaa aatttcgtca gggttttaag ggcatttcgc ataggcggaa
     2761 tattctcttc caagccgtat tcttgctctt gcgtaaattg ggcaaaatcg atttcattca
     2821 gtgttggcaa tgcgttggaa agctcattta acgcaatttc ctctccacta tttatctgcc
     2881 ctttacaata ttcaaacacc tgctttttca cttctcgagt ttgaggggca gataactccc
     2941 cttgctcaca ataatcgctc accgcttgca acaaggtttg attttgcaat tgtggattaa
     3001 acccttcttc cgcacttaaa aaatccataa agaaatcggc aattttacgc cctactctgc
     3061 ctttcacaaa agttaaatag cgatttgacg ttgcgtcaag tttcagttca gttaaattaa
     3121 tgcggcaggc aatatcatat tgtgcaattt cgagatattg cgtgcgttta atctctaact
     3181 gctcatcaac cagcattgaa ttacggctat caatcaacgc aataaacaaa tattcggtag
     3241 ctaaaaaagt gtaacgacac aaaataaaag tgccaccatc ggcaaacgga tatttaatca
     3301 gctctgctgc aagcattttg gcacagctat ggctaaaagg taaaaaatcg gtttcttgct
     3361 ccaacaagcg gttgagctgt tgagcgaaaa ccgactccgg tttaaatata ccgtaagcct
     3421 ttgctttact ttgataggct tgatgtaatt gcaacatcat ttgatcgact tcaggggaaa
     3481 ttgccaataa attttctcgc aaaaacgtat taagctccgg gttctcgccc tctgtttttt
     3541 gtaattgatg taacacgatt tcagaaacat taatgctcat taattcactt ct
//
LOCUS       DANLYI010000210         3569 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.212, whole genome shotgun sequence.
ACCESSION   DANLYI010000210 DANLYI010000000
VERSION     DANLYI010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3569
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.212"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            12..626
                     /locus_tag="PXD81_002243"
     CDS             12..626
                     /locus_tag="PXD81_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HDL5538225.1"
                     /translation="MTLFSDKNDIYSHQVRIVLAEKGVPYEIENVMLGSISEDLLELN
                     PYGNIPTLVDRELVLFNPRIIMEYLDERFPHPPLMPVYPVSRGQCRLNMYRIEQDWYS
                     QIDIVNKDPESAEGKKALAQLKEDVLALAPIFAAKPYFMSDDFSLVDCYIAPLLWRMQ
                     ILGVKFSGATAKPINAYMTRVFQRDSFIQSVGGEAPKHLMDDKD"
     gene            626..1033
                     /locus_tag="PXD81_002244"
     CDS             626..1033
                     /locus_tag="PXD81_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005604075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HDL5538226.1"
                     /translation="MKPLRPYLYNAYYNWIIDSDNTPYLLVNAEFPDVDVPVEFVKEG
                     KIILNISPRSIGQYIVADEYISFSARFQGMLRDIYIPFGAMVAIYAQETGDGIMFQEE
                     AYYTEEAYQARVRAVEQPKKIKAKKTSTLKLVK"
     gene            1230..2189
                     /locus_tag="PXD81_002245"
     CDS             1230..2189
                     /locus_tag="PXD81_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL5538227.1"
                     /translation="MKFHKTLLVTLLSSLSVAALAQDKIQNVAITAIVEHPALDSIRK
                     GVIEELAREGFVDGKNIKIDYQSAQGSTATAAQIARKFVGDKADIIIPITTPSAQPVV
                     AATRSIPIVFSGVTDPVAAKLVKSWEPSGTNVTGISDHKAMAPQVKLIQTLVPDLKAV
                     GYVYSAGEVNSAIVLEELKQEAQKQGFTIVPVAVQRSADIGTAARSLNGKVQAIYISE
                     DNAVVSAYEALHKAALEAKIPVIAADRDTVQRGAVAAYAVNQYDIGVATGKVAARVLK
                     GEKAGTIPIQEVSKLELSINTKTAKELGITIPEVLIKEAKQTF"
     gene            2269..2529
                     /locus_tag="PXD81_002246"
     CDS             2269..2529
                     /locus_tag="PXD81_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005718542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HDL5538228.1"
                     /translation="MGWIAAIIVGAIIGWIASAVMKSEGGLCKNIIIGILGSSLGRWV
                     FGDVLGIGSAYSAGDFNIVGIFFGVLGAALLIFILRKLSIIN"
     gene            complement(2579..3469)
                     /locus_tag="PXD81_002247"
     CDS             complement(2579..3469)
                     /locus_tag="PXD81_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDL5538229.1"
                     /translation="MDKLNAISLFCKVIETQSFTQAAQQAQISLPMASKLVAQLEEHL
                     NVRLLQRTTRKITPTEAGLLYYQQCLPILNELKEADASVSNINSTLQGKITMSVPMDF
                     GSRFIAPYLGKFMEDYPNLALHIEFSDRRVDVIAEGFDLVLRIGSLADSSIVAKKIAD
                     SHLVLVASPDYLARFGEPKGLEALVERDCLLYEYHPQWQFSQQGQKMQIKPQGKIYSN
                     NGYALVQMAKAGLGIINIPKFLIKHELKNGELIPIMPFLTQEPLQISLLYPNRRYLSP
                     KVRILIDFFTQLMQEQKEYI"
BASE COUNT         1086 a          593 c          750 g         1140 t
ORIGIN      
        1 aacgttcggt aatgactctt ttttcggata aaaatgatat ttacagccac caagtgcgta
       61 ttgtgttagc agaaaaaggg gtgccttatg aaatagagaa cgttatgcta ggcagtattt
      121 ctgaagattt attagaattg aatccttacg gtaatattcc tacattggtt gatcgtgaat
      181 tagtgctgtt taaccctcgt attattatgg aatatttaga tgagcgtttc cctcacccac
      241 cattaatgcc ggtttaccct gtttctcgtg gtcagtgccg tttaaatatg tatcgtattg
      301 aacaagattg gtattcgcaa attgatattg tgaataaaga tcctgaatca gcggaaggca
      361 agaaagcgtt agcacagctt aaagaagatg tattggcatt agcgcctatt tttgccgcta
      421 aaccttattt tatgagcgat gatttcagct tggttgattg ctatattgca ccgttattat
      481 ggcgtatgca aatcttaggc gttaaattca gcggtgcaac ggcaaaacca attaatgcgt
      541 atatgacccg tgtattccaa cgtgatagtt ttatccagtc tgttggcggc gaagcgccaa
      601 aacatttaat ggacgataaa gactaatgaa accattaaga ccttatcttt ataatgccta
      661 ctataactgg attattgata gcgataacac gccttactta ttagtgaatg cagaatttcc
      721 agacgtagat gtgccggtag agtttgtcaa agaagggaag attattttaa atatttcgcc
      781 tcgttccatc gggcagtata ttgtggctga tgaatacatt agctttagcg ctcgttttca
      841 gggaatgtta agagatattt atattccttt tggggcaatg gtggcgattt atgcgcaaga
      901 aaccggtgat ggtattatgt tccaagaaga agcctactac actgaagagg cttatcaagc
      961 tcgagttaga gcagtagagc agcctaaaaa aatcaaggcg aagaaaacat ctaccttaaa
     1021 gttggtgaaa taagagatct atttcatcaa aacacattac aagcggttaa atttttctga
     1081 ttatttgcaa atatcagtca aaatttaacc gcttgtttgt tttgtgacgg aaataatcaa
     1141 attccacttg cactccgact aaaaattgct tatctttgtt ttgtgtttgt actgttgtga
     1201 tagtacagta atgatcttaa ggagagaaaa tgaaattcca taaaacctta ttagtgactt
     1261 tgttaagttc actttctgta gcggcattag ctcaagataa aattcaaaat gtagcaatta
     1321 cagcgattgt ggaacaccct gcattagatt caattcgtaa aggtgtgatt gaagaattag
     1381 cccgagaagg gtttgttgat ggtaaaaaca ttaaaatcga ctatcagtct gcacagggta
     1441 gtactgcaac agcagcacaa attgcgcgta aatttgttgg ggataaagca gatattatta
     1501 tcccgatcac cacaccttcg gcacaacctg ttgttgctgc aacacgttct atcccaattg
     1561 tgttttcagg cgtgacagac ccggttgctg caaaattagt aaaatcgtgg gagccaagtg
     1621 gcactaacgt gacggggatt tcagaccaca aagcgatggc tccacaagtg aaattaattc
     1681 aaacattagt accggatttg aaagctgttg gttatgtgta tagtgcaggt gaagttaatt
     1741 ctgcgattgt gttagaagag ttaaaacaag aggctcaaaa acagggcttt actatagtgc
     1801 cggttgctgt tcaacgcagt gccgatattg gcactgctgc tcgcagccta aacggaaaag
     1861 tgcaggctat ttatatttct gaagataatg cagttgtttc ggcttatgaa gccttacata
     1921 aagcggcttt agaagcaaaa attccggtga ttgctgctga ccgtgataca gtacaacgtg
     1981 gtgcagtggc agcttatgcg gttaatcaat atgatattgg tgtggcaacc ggtaaagttg
     2041 ctgctcgtgt attaaaaggg gaaaaagcag gtacaattcc aatccaagaa gtcagcaaat
     2101 tagagctttc aattaatact aaaacggcaa aagaattggg cattactata ccagaggtat
     2161 taatcaaaga agctaaacaa actttttagt gtaaaaaata aaaatttgtt ttatttgtca
     2221 taatcttgta gagtaaaaat tcatttcaac tcagctaagg agaatattat gggatggatt
     2281 gctgcaatta ttgttggcgc tataattggt tggatagctt cggctgtaat gaaaagtgaa
     2341 ggcggacttt gcaaaaatat tattattggt attttgggtt cgtcacttgg acgctgggta
     2401 tttggtgatg tattaggtat tggttccgct tatagtgccg gagactttaa tatagtcggt
     2461 atcttttttg gtgtattagg ggcagctctt ttgattttca ttttgagaaa attaagcata
     2521 attaactaat ttcgtatttc agaaaggaag cagttgtttt ataactgctt cttttttgtt
     2581 aaatatactc tttttgctct tgcatcagtt gtgtgaaaaa atcaattagg attcgcactt
     2641 taggagagag ataacgacgg tttggataga gtaagctgat ttgtaaaggt tcctgtgtca
     2701 aaaaaggcat aataggaatc aactctccgt tttttagctc gtgttttatt aaaaactttg
     2761 gaatgttaat gatgcccaaa ccagcttttg ccatttgtac taacgcataa ccattgttac
     2821 tgtaaatttt accttgtggt ttaatttgca ttttttgtcc ttgttgagaa aattgccatt
     2881 gaggatggta ttcatagagt aggcagtctc gttccactaa tgcttctaag cctttaggtt
     2941 cgccaaatcg agctaaataa tctggagaag caactaaaac caaatgggaa tctgcaattt
     3001 ttttcgctac aatggaacta tctgcaaggc taccaatacg cagcaccaaa tcaaagcctt
     3061 ctgcaatcac atctacccga cggtcactaa attcaatatg aagggctaaa ttagggtaat
     3121 cttccataaa tttcccaagg taaggggcaa tgaagcgaga gccaaaatcc attggtacag
     3181 acattgttat tttcccttgt agcgttgagt taatattact tacgctggca tcggcctctt
     3241 ttagttcatt taaaataggc aagcattgct gatagtagag caaccctgcc tcagttggcg
     3301 taatttttcg agtggttcgt tgtagtaaac gcacattgag atgttcttct aactgagcca
     3361 ccaatttact tgccatgggt aacgaaattt gagcttgttg tgctgcttga gtgaaacttt
     3421 gggtttcaat tactttacaa aataaactga ttgcgtttag tttatccatt ttctctcttc
     3481 tttatattgt aatttgtgtt aaggcttgct atcgtaaaaa tagtgtctaa aaaaatcaac
     3541 ttaaaaagaa ataatttcaa acaatttaa
//
LOCUS       DANLYI010000211         3550 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.213, whole genome shotgun sequence.
ACCESSION   DANLYI010000211 DANLYI010000000
VERSION     DANLYI010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3550
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.213"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            78..1148
                     /gene="dhaK"
                     /locus_tag="PXD81_002248"
     CDS             78..1148
                     /gene="dhaK"
                     /locus_tag="PXD81_002248"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012073177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HDL5538230.1"
                     /translation="MKKLINSIETVLDEQLSGFVKAHSDLTLNTEPVYIYRTTPSNQP
                     KVALISGGGSGHEPMHAGFIGKGMLDGACPGAIFTSPTPDQMFECGLAVDRGEGVLLI
                     IKNYTGDVLNFETATELLADSGVKVATVLVDDDVAVKDSLYTAGRRGVANTVLIEKLL
                     GAAADKGYNLSELAKLGHQLNNNGHSIGIALSACTVPAAGKPSFTLAENEMEFGVGIH
                     GEPGIERRPFENLDSTVKQMFETLIAHGDYQRKIRHWNTQTQQWDEIEESKVALKKGD
                     RVIALVNNLGAVPLSELYAVYDALEKECQTFGLSIERSLVGSYCTSLDMQGISITLLK
                     VDEDILKLWDAPVNTPAMRWGE"
     gene            1169..1795
                     /gene="dhaL"
                     /locus_tag="PXD81_002249"
     CDS             1169..1795
                     /gene="dhaL"
                     /locus_tag="PXD81_002249"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase ADP-binding subunit
                     DhaL"
                     /protein_id="HDL5538231.1"
                     /translation="MTISKQQILKWLENCQQVMTEKRDYLTELDTVIGDGDHGLNMQR
                     GFSKALEKAHTVQDKDIGTLLKTVGMTLLSQVGGASGPLYGSFFIKAAQGATGKEQLS
                     FNELLALLQQGAEAIVQRGHAQVGDKTMCDLWLPLLEDLAQADSTQPIAQILLQAVEK
                     AENFANQTAPLMAKKGRASYLGERSIGHKDPGATSSYYIFKALAEALQ"
     gene            1805..2212
                     /gene="dhaM"
                     /locus_tag="PXD81_002250"
     CDS             1805..2212
                     /gene="dhaM"
                     /locus_tag="PXD81_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HDL5538232.1"
                     /translation="MVNLVIVSHSAKLAEGVGEILAQMAQGGCKIALAGGIEDTSNPI
                     GTDAVKIMQAIESVFSPDGVIVFVDLGSAILSTQTAIDLLEPELAEKVIISYAPLVEG
                     AFAAVVAASAGDSLEAVLNEANEAAALKQEQAN"
     gene            complement(2252..3097)
                     /locus_tag="PXD81_002251"
     CDS             complement(2252..3097)
                     /locus_tag="PXD81_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone YbbN"
                     /protein_id="HDL5538233.1"
                     /translation="MDYFVNVNEQNFSEVWAAAAQVPTVFHFISPREVASLEMDALLR
                     RISDENPNQFLLATVNCDEQQALAAQFRIQQIPTLYLFAGGQPIDAIQGAVSEQELRI
                     RLANILPKEEELKFQQALELLQDQRYTEALPLLKSAWEMTDKKNSDFALLYAETYIAM
                     KSVEEAREILKAIPIQDRDSRWHGLQAQIELLEQASESPELQQLQADYANSKTPEIAI
                     KLANQLHQAHKNEEALTLLFDWLKSDLNVANGEVKSQFLAILSALGTDPIVPKFRRQL
                     YSLLY"
     gene            complement(3141..3467)
                     /gene="glpE"
                     /locus_tag="PXD81_002252"
     CDS             complement(3141..3467)
                     /gene="glpE"
                     /locus_tag="PXD81_002252"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005595720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HDL5538234.1"
                     /translation="METTFKEISPQQAWELIENEGAVLADVRDARRYVYSHPQDAFHL
                     TNQSYGKFLDEVDYDEPVVVICYHGVSSQSTAQFLIEQGFENVYSVKGGFEAWERNGL
                     PIETSY"
BASE COUNT         1049 a          728 c          776 g          997 t
ORIGIN      
        1 tgtgacaaag ttaacagaaa tccgactgtg tttcttgtaa attgtgacag tctaattcat
       61 ttaaaagggg caaaaagatg aaaaaattaa ttaattcaat agaaaccgtc ttagatgagc
      121 aactttctgg ttttgtgaaa gcacattctg atttaacact taacacagaa cctgtctata
      181 tttaccgcac aacgccaagt aatcaaccta aagtagccct tatttccggt ggtgggagtg
      241 ggcacgagcc aatgcacgcc ggttttatcg gtaaaggaat gttagacgga gcttgccccg
      301 gagcaatctt tacatctcca acgccggatc aaatgtttga atgcggttta gcggttgatc
      361 gtggcgaggg tgtattgcta attattaaaa actataccgg tgatgtgctg aattttgaaa
      421 cagccaccga gctacttgca gatagcggtg taaaagtcgc aaccgtgttg gttgatgatg
      481 atgtggcagt caaagacagt ttatatactg ccggccgccg cggtgttgca aatacagtat
      541 taattgaaaa gctgttagga gctgcagcgg ataaaggcta taacttatcc gaattagcca
      601 aattagggca tcagttaaac aataacgggc attctatcgg aattgcctta tctgcttgta
      661 ctgtgccggc tgccggtaag ccatcattta ctttagctga aaatgagatg gaatttgggg
      721 taggtattca cggagaaccg ggcattgaac gtcgcccctt tgaaaatctg gatagtaccg
      781 tgaaacaaat gtttgagacc ttaattgctc acggcgatta tcaacgtaaa attcgccatt
      841 ggaatacaca aacccaacaa tgggacgaaa tcgaagaaag caaagtggca ttgaaaaaag
      901 gtgatcgtgt tattgcttta gttaataact taggagcagt gccactatcc gaactttatg
      961 ctgtttatga tgcgttagaa aaagagtgcc aaactttcgg cttaagcatt gaacgcagtc
     1021 ttgttggatc atattgcacc tcgctggaca tgcaaggtat ctctatcaca ttactgaaag
     1081 ttgatgagga tattctcaaa ctatgggatg ctccggtaaa tacaccggca atgcgttggg
     1141 gtgaataatt agattgaaag gataagttat gacaatatca aaacaacaga tcttaaaatg
     1201 gcttgaaaat tgccaacagg taatgactga aaaacgggat tatttaaccg aattggatac
     1261 cgttattggc gatggcgatc acggtttaaa tatgcagcgt ggatttagta aagcacttga
     1321 aaaagcccat accgtgcaag ataaagatat cggcaccctg cttaaaaccg ttggaatgac
     1381 attattatcg caagtagggg gagcgagcgg ccctctctat ggttcatttt ttattaaagc
     1441 ggctcaaggt gctacaggca aagagcaact gagctttaac gagttactgg cacttctaca
     1501 gcaaggagca gaagctatcg tacaacgtgg acacgcacaa gttggcgata aaacgatgtg
     1561 tgatttatgg ctgccattac tcgaagactt agctcaagcc gatagcactc aaccaattgc
     1621 tcaaatctta ttacaagcgg tcgaaaaagc agaaaatttt gcaaatcaaa ccgctccatt
     1681 aatggcgaaa aaagggcgtg ccagttactt gggcgaacgc agtatcgggc ataaagatcc
     1741 gggagcaacg tccagctact atatttttaa agccttagct gaagcattac agtaggaggg
     1801 gtaaatggtt aatttagtga ttgtttcaca tagtgctaaa ctggcggaag gcgtggggga
     1861 aattttggct caaatggcac aggggggctg taaaatcgcc cttgccggcg gcattgagga
     1921 tacctcaaat cctatcggca ctgatgcggt aaaaataatg caagcgattg aatcggtatt
     1981 tagcccagac ggtgtgattg tgtttgtcga tttaggcagt gcaattttaa gtacccaaac
     2041 cgccatagat ttactggagc cggaacttgc cgaaaaagtg atcattagct acgctccttt
     2101 agttgaaggt gcttttgctg ctgtagttgc agcctctgcc ggcgatagtt tagaagcggt
     2161 attaaatgaa gcgaacgaag ccgctgctct taagcaggaa caagccaatt agtaaaaatg
     2221 ccgtatcgaa aaatacggca ttttagtttt attagtacaa tagtgaataa agctgtcttc
     2281 taaatttcgg cacaatcggg tctgtaccca gtgcagataa gattgccaga aattggcttt
     2341 ttacctcgcc gttcgctaca tttaaatccg attttaacca atcaaaaagt aacgtgagtg
     2401 cttcttcatt tttatgagct tggtgtagct gatttgccaa tttaatcgca atttcagggg
     2461 ttttgctgtt cgcataatcc gcttgaagct gttgtaattc gggcgattcg ctggcttgtt
     2521 ccaataattc gatttgggct tgcaaaccgt gccaacggct gtctctgtct tgaatcggga
     2581 tcgctttaag gatttcacga gcctcttcca cgcttttcat tgcaatgtaa gtttcggcat
     2641 agagcaaagc gaaatcgctg tttttcttat cggtcatttc ccacgctgat ttcagcagcg
     2701 gcaaggcttc ggtataacgc tgatcttgca ataattccaa ggcttgttga aatttcagct
     2761 cttcctcttt cggtaaaata tttgccaacc gaatccgtaa ttcctgctca cttactgcac
     2821 cttgaattgc atcaatcggc tgaccgccgg caaacaaata gagcgttgga atttgttgaa
     2881 tgcgaaattg agcagccaat gcttgttgct catcacaatt taccgtcgcc aataaaaatt
     2941 ggttagggtt ttcatccgaa atacgacgta gtaaggcatc catttccaat gaagccactt
     3001 cccttggcga aataaaatga aacaccgtcg gcacttgtgc cgctgctgcc catacttcac
     3061 taaaattctg ctcgtttaca tttacaaagt agtccataca aaaccttctt aagctaaaaa
     3121 ttacttttta tattgtagca ttaataagaa gtttcaatag gtagcccgtt tctctcccac
     3181 gcttcaaaac caccttttac actataaaca ttctcaaacc cttgttcgat taaaaactgt
     3241 gctgtacttt ggctactcac tccgtgatag caaatcacaa ccaccggttc gtcataatcg
     3301 acctcgtcta aaaacttacc ataactctgg ttggttaaat gaaacgcatc ctgtgggtgg
     3361 ctgtaaacat aacggcgggc atcacgcaca tcggcaagca ccgcaccttc gttttcgatt
     3421 aattcccaag cctgttgcgg gctaatttct ttaaatgtag tttccataat cactttccct
     3481 caaaaaacgt gcaactatac cataaaattc taaaaagaat ggctattatt ccaagttgaa
     3541 aatttgatgt
//
LOCUS       DANLYI010000212         3541 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.214, whole genome shotgun sequence.
ACCESSION   DANLYI010000212 DANLYI010000000
VERSION     DANLYI010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3541
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.214"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            complement(<1..2537)
                     /locus_tag="PXD81_002253"
     CDS             complement(<1..2537)
                     /locus_tag="PXD81_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005614417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1A"
                     /protein_id="HDL5538235.1"
                     /translation="MRIAKIIFSALLSLLVLGGVIGGLLYMHFKSDLPDVATLKNVEL
                     QQPMQIFTLDGKLIGEVGEERRIPVKLDDIPPMLVKAILATEDARFYEHKGIDPKGIM
                     RAIWRTSQGDTQGASTITQQLAKNFFLTPERSLERKAKEAILALEIEQVLSKNEILEL
                     YLNKIYLGYRSHGVAAAAKTYFNKSLKDLTLSEIAIIAGLPKAPSTMNPLYSVKRAED
                     RRNVVLGRMLETGDITKEEYEKAKAEPVVANYYGATLEFRADYVTEMVRQEIVKRYGE
                     NIAYNKGLKVYATVLSADQKAAQDALRENLIDYDRRHGWRGAEKLWTGSSAWDDEKII
                     DHLSKLPKSEPFTPAVVMQADKSKYTILLANGDISVLKRTNASFAPKGLKAGEQIWVR
                     QNKNREWALGQIPEVNSALVSINSENGAIESIVGGFSFEQSRFNRATQSLVQVGSAIK
                     PFIYTAAMNKGLSLSTTISDSPIVIKKKGQKEWRPKNADGVYGGPLRTRVALGKSKNM
                     VAIRILQMAGIDYVADYLQRFGFNRNQYVATESLALGAASFTPLEMARAYAVFNNGGY
                     LIEPYIIDRIIDAQGYELYQANPVVACNSCDSPVVYPEPKYFESVKIHDEAQVGVQTI
                     ANSSNNDDDDVVEDVGDSEPELQANNSEKNAPSLMAESSHHIKGLRYAPHVISDELAF
                     LMRSALATAITGEPEYGWRGTSYKMLNTIKRADVGGKTGTTNNAKATWYAGFGANIST
                     VVYVGFDDNKRVLGRGASGSSTALPAWTNYMKVALSDKPVEKSTLPKNIIEVNIDPNS
                     GFLGNGRKEYFIKGTEPTKRYIVERAYTEKAPQKTESAPLRLGLPPPG"
     gene            2661..3359
                     /locus_tag="PXD81_002254"
     CDS             2661..3359
                     /locus_tag="PXD81_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComA"
                     /protein_id="HDL5538236.1"
                     /translation="MKLLKAHSKTPSNRIILGLSEDEQRYCLVKNKAEKFTVFWQAKP
                     ASIEELIGNLTPQEQAMMTLVRPIPYQYIWRKTVFMANTLNEAQLHRKVIHILKNEQP
                     LALDLLNIDYQRFPSSNDNLDKVVIYAVRKSYTENLSQFNSILDCELHCYARAIIHLT
                     QSQNNQPAPCFSFKQKYFQFTETELLILQTEPEDCIHLSDLKIDHLEIECEQEKQLYC
                     LALGASLWNGKVLI"
     gene            3335..>3541
                     /locus_tag="PXD81_002255"
     CDS             3335..>3541
                     /locus_tag="PXD81_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5538237.1"
                     /translation="MEWQGIDLSANRKNNWFAGSDKGIKNVIYTLLLSALISLALIFL
                     YLQKNQEIQPLAQQLELVKTDILNI"
BASE COUNT         1068 a          784 c          598 g         1091 t
ORIGIN      
        1 cctggtggag gtaatcctaa acgtaacggc gcagattctg ttttttgagg cgctttttca
       61 gtataagctc tctctacaat ataacgtttt gtaggctcag tacccttaat aaaatactct
      121 ttacgtccat ttcctaagaa accactatta gggtcaatat taacctcaat aatatttttt
      181 ggaagggtac ttttttcgac aggcttatct gacaatgcaa ctttcatata atttgtccaa
      241 gctggtaaag cagtactaga acctgaagca cctctaccta atactctttt attatcatca
      301 aagccaacgt acacaacagt agaaatatta gcaccaaatc ccgcatacca agttgccttg
      361 gcattattgg ttgtaccagt cttcccgccc acatccgctc gttttatcgt attgagcatt
      421 ttatagcttg ttcctcgcca gccatattca ggctcaccag taattgctgt agctaaagca
      481 ctacgcatta aaaaagcgag ttcatcacta ataacatgag gagcgtaacg taaccctttg
      541 atatgatgag aagattctgc cattaagctc ggcgcatttt tctcgctatt gttcgcttgt
      601 aattcaggtt ctgaatcgcc aacatcttcc acaacatcgt cgtcatcatt attcgaggaa
      661 ttcgctatag tttgtacacc gacttgagct tcatcatgaa ttttgactga ttcaaaatat
      721 ttaggctcag ggtaaacaac tgggctatcg caactattac aagctactac aggatttgct
      781 tgatataact cgtagccttg ggcatcaata atacggtcaa tgatatatgg ttcaatcaaa
      841 taacctccat tattaaatac tgcataagct cgagccattt ctaatggcgt aaaagatgca
      901 gcacccaatg caagagattc tgttgcaaca tactgatttc ggttaaagcc aaaacgttgt
      961 aagtagtctg caacataatc aatccctgcc atttgtaata tacgaatagc caccatattt
     1021 ttcgatttac ctaatgcaac acgagttctc aacgggccgc cataaacccc atcggcattt
     1081 ttaggtcgcc actctttttg ccctttcttt ttaatgacaa taggagaatc gctaattgtt
     1141 gttgaaagac ttagcccttt attcattgcc gctgtgtaaa taaacggttt aatcgcagag
     1201 cctacctgaa ctaatgactg agtcgcacga ttgaaacggc tttgttcaaa gctaaagcct
     1261 ccaacgatag attcaatggc accgttttca ctattaattg acactagggc tgaatttacc
     1321 tccggaattt gtcctaaagc ccactcccta tttttatttt gacgtaccca aatttgttca
     1381 cctgctttta accctttagg tgcaaaagaa gcatttgtac gttttaaaac agagatatca
     1441 ccatttgcca ataaaattgt atattttgat ttatctgctt gcattacaac agctggtgta
     1501 aacggctctg attttggcag cttacttaaa tgatcgataa ttttctcatc gtcccaagca
     1561 gaactacctg tccataattt ttcagcgcct cgccaaccat gacggcggtc ataatcaatc
     1621 aaattttcac gtaacgcatc ttgtgctgct ttttggtctg ctgaaagtac cgtggcgtac
     1681 acttttaagc ctttgttata agcaatattt tctccatagc gtttcacaat ttcttgacga
     1741 accatttctg tcacataatc cgctcgaaat tctaatgtcg caccatagta gttagctact
     1801 actggctctg ctttagcttt ctcatattcc tcttttgtaa tatctccggt ttctagcatt
     1861 ctcccaagca ctacattacg acggtcttcc gcccgtttta ctgaataaag tggattcatc
     1921 gttgaaggcg cttttggaag ccctgcaata attgctattt cagaaagggt taaatctttc
     1981 aaagatttat taaaataagt tttagccgca gcggcaacac cgtgagaacg ataccctagg
     2041 taaattttgt ttaaatacag ctccagaatc tcatttttac tgagaacctg ttcaatttct
     2101 aaagctaaaa ttgcctcttt ggccttacgc tctaaactac gttcaggtgt taagaaaaag
     2161 tttttcgcca gctgctgggt gattgtactt gcgccttgag tatctccctg gctagtgcgc
     2221 catattgcac gcataatacc tttaggatcg atccctttat gctcgtaaaa acgtgcatct
     2281 tcggtagcaa gaatcgcttt tactaacatc ggtggaatat catcaagctt gaccggaata
     2341 cggcgttctt ctcctacttc accaattaat ttaccatcta aggtaaaaat ctgcataggc
     2401 tgctgtaact ctacattttt cagcgttgcc acatcaggca aatcagattt gaaatgcatg
     2461 tacagtaaac caccaattac cccacctaaa accaagagtg aaagtaaagc actaaatatt
     2521 atttttgcga tccgcatcga aaaaatctct tttggttaat gacgaaacat aaatttaaag
     2581 ccaatattag gtcactaagt ataagctaaa aacgcttact tcgcataact aattgcaaaa
     2641 ctttccttac tatacttact atgaaattac ttaaagcaca ttcaaaaaca ccttcaaacc
     2701 gtatcatctt aggcttaagt gaagatgaac aacgttactg cttagtcaaa aataaagcag
     2761 aaaaattcac cgttttttgg caagccaagc ctgcgagcat cgaagagctt atcggtaatc
     2821 tcaccccaca agaacaagca atgatgacgc tggttcgccc gatcccttac caatacattt
     2881 ggcgaaaaac cgtttttatg gctaacaccc tcaatgaagc tcaattacac cgtaaggtaa
     2941 ttcacatttt aaaaaatgag cagccacttg cattggatct actcaatatt gactatcagc
     3001 gatttccaag cagcaacgat aatctggata aagtggttat ctacgccgtg cgaaaaagtt
     3061 ataccgagaa tttgagccaa tttaacagca tcttagattg tgagctgcat tgctatgcac
     3121 gggctataat tcatttaaca caatcccaaa ataatcagcc tgcgccctgt ttttcattta
     3181 aacagaagta cttccaattt accgagacgg aattactgat attgcaaaca gaacctgagg
     3241 attgtattca tctatctgat ctcaaaattg accatcttga aatcgagtgt gagcaagaga
     3301 aacagctata ttgcctcgcc ttaggagcaa gtttatggaa tggcaaggta ttgatttaag
     3361 tgctaatcga aaaaataact ggtttgccgg cagtgataaa ggcatcaaaa acgtgattta
     3421 tacactttta ttatctgccc tcattagctt ggctctgatt tttctctatt tgcaaaaaaa
     3481 tcaagaaatt caaccgcttg cacagcaatt agagctggta aaaaccgata tattaaatat
     3541 t
//
LOCUS       DANLYI010000213         3530 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.215, whole genome shotgun sequence.
ACCESSION   DANLYI010000213 DANLYI010000000
VERSION     DANLYI010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3530
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.215"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            <1..2004
                     /locus_tag="PXD81_002256"
     CDS             <1..2004
                     /locus_tag="PXD81_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HDL5538238.1"
                     /translation="TSQAELNAIEVKEKLAATTGYIPVYTTVGTKTLTSLARTPFSIN
                     VVSDDLMHDQAVRSVTDSVAYTSGMMGGYRGNNSLIEISIRGIGNKSDGSTIPTYLNG
                     SRYQTAFEIDPFFLESINVIKGPNSILYGQANPGGVMDIITKKAGSNPHRHLQFVVGN
                     HQRYQIGLDIEENLSNTLSARLIGQLERTNWNTQFVKEKGGAFAPSILWKPTTNTELN
                     VYAYYAKKPKAGDRNFLPKEGTINSVNGKKLPYDMFLSDLNYHGLKAEQMQVGYSFSH
                     KFTPNLTFRQNASYHNGEENFKNLVYWGDVGSVLERRARHWDVKTQEINLDNQLEWVF
                     STANINHTLLAGIDYKRVRKDLDNYMGIAPDFDWQNPVYGVTVTEPSLFSSEVKHLKQ
                     LGVYLQDQIEIGNFDFLLGGRYDRAKNSNQDRLNSKTENNQEGKFTWRTGVVYNFENG
                     IAPYISYSTSFVPSAEKDAQGNFLKPTTAGQWEVGMKYQPTQDTLLTLAGFKITQKDL
                     ANYQWQTRSYEQIGEVQTKGIEVSLNQQLSDKFSIAASYAYLKKEITADKDGTTIGKT
                     QWGVPRHQASLWAKYKFFDKLTAGVGVRYMGTTFGDNQNTFTVPAYTLYDMMLAYDFM
                     PNLNLQLNAQNLTNKKYVASCANNFSCFYGKDRNLSATLNYHF"
     gene            2048..3484
                     /locus_tag="PXD81_002257"
     CDS             2048..3484
                     /locus_tag="PXD81_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3327 domain-containing protein"
                     /protein_id="HDL5538239.1"
                     /translation="MKKTTACILTFLVSSVLYANNITIELKQNDFVQGKLIQKAQQPL
                     SLSIIFPDQSSRLLAQNVFGEQDFMFKSELIGTATFSILANGATVSDQDFTLTIERHI
                     PISAQIAPPKVIENTQLEELSQKIAKNPEKQTALINDFWDKAKQAGTPLIEPLDEQQS
                     RVTFLWRGATKNARIWGGVSTEHDFMQRFGETDIWYKSYIVPNDTLVEYKLAPDVPTL
                     PVDENTQRRALLATAQADPLNKTAYFEKIGQNIHNTDKFNYASLLKLPNAPTQSFLTE
                     APNIAKGKLQQLIFESATLGNKHRLFVYLPDGFSAEKNYAVLYLFDGVEYTERVPTKT
                     ILDNMIAQKVILPTVAVFIENPSSESRRVELPANPIFTQVLAKELVPFVEKQLNIKTK
                     ERIIGGSSYGGLSSAYTVLNYPNVFSKALILSGSFWWQPKGTPSEQSHYIAQQFIQKE
                     KLPLCFFISAGFYESGKSAICKRSNYFA"
BASE COUNT         1166 a          711 c          672 g          981 t
ORIGIN      
        1 acctctcaag cagaactaaa tgctattgag gtcaaagaga aattggcagc taccaccggc
       61 tatattcctg tttatacgac tgtaggaacg aaaacattaa cctctttggc tcgtacacct
      121 tttagtatca atgttgtctc tgatgattta atgcatgacc aagctgttcg ttctgttacc
      181 gattcagtgg cttatacctc cggtatgatg ggtggctatc gtggtaacaa ttcgcttatc
      241 gaaatctcca ttcgaggcat tggcaataaa agtgatggca gtacaattcc gacctattta
      301 aacggttctc gctaccaaac tgcatttgaa atcgatccgt tcttcttaga aagtatcaat
      361 gtgattaaag gtccaaactc cattttatac gggcaagcta atcccggtgg cgtaatggat
      421 attatcacca aaaaagccgg cagtaaccca catcgccatt tgcaatttgt ggtgggaaac
      481 catcaacgat accaaatcgg gctggatatt gaagaaaatc tctccaatac cttatccgct
      541 cgtttaatcg gtcaattaga acgaacaaac tggaataccc aatttgtaaa agaaaaaggc
      601 ggtgcttttg ctccaagcat tttatggaaa ccgactacaa atacagaact taatgtttac
      661 gcttactatg caaaaaaacc gaaagccggt gatcgtaact ttttaccgaa agaaggaacg
      721 attaatagcg taaacgggaa gaaactaccc tatgatatgt tcctcagcga tctgaactat
      781 cacggtttaa aagccgaaca aatgcaagta ggttacagtt tctcacataa atttacgcca
      841 aacctaacgt tccgccaaaa tgcaagttac cataatggcg aagagaattt caaaaatcta
      901 gtttattggg gcgatgtcgg ttcggtttta gaacgtagag ctcgccattg ggatgtcaaa
      961 acccaagaaa tcaacttaga taaccaatta gaatgggtat ttagcacagc aaatataaat
     1021 cataccttgt tagccggtat tgattataaa cgagtgcgta aagatcttga taactatatg
     1081 gggattgccc ctgattttga ttggcagaac cctgtttatg gcgtgaccgt tactgagcct
     1141 agcttatttt caagtgaagt gaagcattta aaacaacttg gggtttatct acaagatcaa
     1201 atagaaatcg gtaattttga cttcctgtta ggcggtcgtt acgatcgagc aaaaaacagc
     1261 aaccaagatc gtttgaacag taaaacagag aataaccaag aaggaaaatt tacttggcgt
     1321 acaggcgtag tttacaactt tgaaaacggt atcgctccgt atatcagcta cagcacttct
     1381 tttgtgccat cggctgaaaa agatgctcaa ggaaatttct taaaaccaac aactgctggg
     1441 caatgggaag tgggaatgaa ataccaacct acccaagaca ccttactgac cttagcagga
     1501 tttaaaatta cacaaaaaga tttagccaat tatcaatggc aaacccgctc ttatgagcaa
     1561 attggtgaag tgcaaaccaa aggaattgaa gtgagtttaa atcagcaatt atccgataaa
     1621 ttttcaatag cggcttctta tgcttactta aaaaaagaga ttactgcaga taaagatggc
     1681 acgacgattg gaaaaacgca gtggggagtg cctcgccatc aagcatctct ttgggcaaaa
     1741 tataaattct ttgataagct gaccgccggt gtaggtgtac gctatatggg aacgactttt
     1801 ggcgataacc aaaatacttt taccgtaccg gcatacacac tttacgatat gatgctagct
     1861 tatgatttta tgcctaactt aaacttacag ctcaacgcac aaaacctgac gaataaaaaa
     1921 tatgtcgcaa gctgtgccaa taatttttcc tgtttctatg gaaaagatcg caatttaagt
     1981 gcaacgttaa actatcattt ttaatctcaa cagccctcaa atgagggctt aaacttttgc
     2041 aaaaaatatg aaaaaaacga ccgcttgtat tcttactttt ttggtttcat ctgtgcttta
     2101 tgctaacaat ataaccattg agcttaaaca gaatgatttt gtgcaaggca aattgattca
     2161 gaaggctcaa cagccacttt ccttgtcgat tatttttcct gaccaaagct ctcgcctgct
     2221 ggctcaaaat gtctttggtg aacaagattt tatgtttaaa tcagaactaa tcggcacagc
     2281 tactttttct attctagcga atggtgcaac cgtttcagac caagatttta ccttaacgat
     2341 tgagcggcat attccaatct cggcacaaat tgctccacct aaagtaattg aaaatacaca
     2401 gcttgaagaa ctcagccaaa aaattgcaaa aaatccagaa aaacagaccg ctttaattaa
     2461 cgatttttgg gataaagcaa aacaagccgg cacaccattg attgaaccat tggacgaaca
     2521 gcaaagccgt gtaacttttt tatggcgtgg agcaacgaaa aatgcccgta tttggggcgg
     2581 tgtgagtact gaacacgatt ttatgcaacg ctttggtgaa acggatatat ggtataaaag
     2641 ttatatagtg cccaatgaca ctttagtgga atacaaatta gctccagatg tgccgacatt
     2701 gcccgttgat gagaataccc aacgcagagc gttactggct acggctcaag ccgatccact
     2761 caataaaaca gcatacttcg agaaaatcgg acaaaatatt cataataccg ataaattcaa
     2821 ctacgcttcg ctacttaaat tgccaaacgc acctactcaa tcttttttga ctgaagctcc
     2881 aaatattgca aaaggtaaac tgcagcagtt gatttttgag agtgcaacac tcggcaataa
     2941 acacagatta ttcgtctatc tgcctgacgg ttttagtgca gaaaaaaact atgcggttct
     3001 ctatttattt gatggtgttg aatataccga acgagtgcca actaaaacca ttttagataa
     3061 tatgatcgct cagaaagtga ttctgcctac tgtagcggtg tttatcgaaa atccatcgag
     3121 tgaatcaaga cgtgtcgagc tgcctgccaa tcccatattt acgcaagtat tggcaaaaga
     3181 acttgtacct tttgttgaaa aacagctcaa tataaaaaca aaggagcgaa ttattggcgg
     3241 ctctagctat ggcgggctat cctctgctta caccgtactc aactatccta atgtatttag
     3301 taaagcattg attctatccg gttcattttg gtggcaaccc aaaggcacac ctagtgaaca
     3361 aagccactat attgctcaac aatttatcca aaaagaaaaa ttaccactct gttttttcat
     3421 cagtgcaggt ttttatgaaa gcgggaaatc ggctatttgt aagcggtcga attattttgc
     3481 atagaattac actcaatttt gcatagataa aaattaaaaa ctatgcaaaa
//
LOCUS       DANLYI010000214         3437 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.216, whole genome shotgun sequence.
ACCESSION   DANLYI010000214 DANLYI010000000
VERSION     DANLYI010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3437
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.216"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            complement(<1..795)
                     /locus_tag="PXD81_002258"
     CDS             complement(<1..795)
                     /locus_tag="PXD81_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chain length determination protein"
                     /protein_id="HDL5538240.1"
                     /translation="MADSVKSHEQSSDEIDLIEIFRALWDKKWWIVITTFITTLLAGI
                     YAFTAKEQWTSKATVIAPKLTELGEYLNVRREYSRILRVDPVDPNALSASLFNNFNRL
                     AQSQDTKNEFFITSNVYKTLAEGKDEQGQRTVLSNLSNENTSFIIPDAKKDPNAIGRT
                     LSFSAETPMEAQDTLGKLVQFINSKAFAVELEDFLVDFQNVLTALQYEKSLIQEDLSV
                     NKSVQLENLNKAYETAQKAGIKEYSKAFNQSDASNIAAALSDTKISL"
     gene            944..1285
                     /gene="erpA"
                     /locus_tag="PXD81_002259"
     CDS             944..1285
                     /gene="erpA"
                     /locus_tag="PXD81_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005706127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HDL5538241.1"
                     /translation="MSDIQIPLIFTDAAARKVKSLIEGEENPNLRLRVYITGGGCSGF
                     QYGFTFDDQVNEGDLTIENQSVGLVVDPMSLQYLIGGTVDYTEGLDGSRFVVNNPNAS
                     STCGCGSSFSI"
     gene            complement(1390..1713)
                     /gene="yidD"
                     /locus_tag="PXD81_002260"
     CDS             complement(1390..1713)
                     /gene="yidD"
                     /locus_tag="PXD81_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HDL5538242.1"
                     /translation="MVKTHSPEQKAGTTESINQPPRLSLLAKILLLPIYFYRYAISPL
                     IGPRCRFYPTCSTYAVEAIKIHGAIKGGYLAFKRILRCNPLSEGGEDPVPLCCKGNCK
                     HNDKK"
     gene            complement(1662..2036)
                     /gene="rnpA"
                     /locus_tag="PXD81_002261"
     CDS             complement(1662..2036)
                     /gene="rnpA"
                     /locus_tag="PXD81_002261"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HDL5538243.1"
                     /translation="MKKLNFSRELRLLAPVQFKAVFEEPLRASTPQMTLLARENNLEN
                     PRLGLTVAKKHLKRAHDRNRIKRIVRESFRLKQHELPNFDFVFVAKGGIGKLDNATLF
                     ETLEKLWSRHIRLSKKPAQQNP"
     gene            complement(2072..2206)
                     /gene="rpmH"
                     /locus_tag="PXD81_002262"
     CDS             complement(2072..2206)
                     /gene="rpmH"
                     /locus_tag="PXD81_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011508608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HDL5538244.1"
                     /translation="MKRTFQPSVLKRARTHGFRARMATKNGRQVLARRRAKGRKSLSA
                     "
     gene            2443..3405
                     /gene="hemH"
                     /locus_tag="PXD81_002263"
     CDS             2443..3405
                     /gene="hemH"
                     /locus_tag="PXD81_002263"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HDL5538245.1"
                     /translation="MNQPQQIGILLANLGTPDEPTPSAVKRYLKQFLSDPRVIDLPKF
                     KWQAILNCIILPRRSPKVAKLYQSVWSEEGSPLLAISRQQQRAVQRYFDAKGKNVVVE
                     LGMSYGNPSIESATDNLIKQGVERIIVLPLYPQYSSTTTASVFDAFANGLKAQRKIVP
                     FDFIHSYHNDPLYIQALANSIDVKPDELLLFSFHGIPKRYETEGDFYAKHCQETAELV
                     AEKLGLPREKWYLSFQSRFGDEEWLQPYTDQTLESFPQKGIKKVAVVCPGFSADCLET
                     LEEITEENRENFEHAGGESYRYIPALNASGDHIKALVKLIEAKI"
BASE COUNT         1041 a          659 c          687 g         1050 t
ORIGIN      
        1 aagggagatt ttagtatcac ttaaagctgc tgcaatattt gatgcatctg attgattaaa
       61 tgcttttgaa tattctttaa tacctgcttt ttgtgcagtt tcataggctt tatttaagtt
      121 ttccaactga accgacttat tcacagataa atcttcctga attaatgatt tttcatattg
      181 taatgcagta agcacatttt ggaaatcgac tagaaaatct tctaattcaa cagcaaatgc
      241 tttgctatta atgaattgaa ccaacttacc aagggtgtcc tgcgcctcca taggtgtctc
      301 tgcagaaaaa gatagtgtac gacctattgc attaggatct ttctttgcat caggaataat
      361 aaagctggta ttttcattac tcaaattgga taaaactgtg cgttgcccct gctcatcttt
      421 tccttcagct aacgtcttat acacattgga agtgataaaa aattcatttt tagtatcttg
      481 agattgtgct aatcggttaa aattgttgaa caaactcgca gacaatgcat ttggatcaac
      541 aggatctaca cgcaaaatcc gactatattc tctgcgaaca tttaaatact cacctaactc
      601 tgttaattta ggggcaataa ctgttgcctt agacgtccat tgctcttttg cagtaaatgc
      661 ataaatacca gcaagtaatg tggtaatgaa tgttgttata acaatccacc attttttatc
      721 ccatagagca cgaaaaatct caattaaatc aatctcatcc gagctttgct catgactttt
      781 tactgaatct gccatagttt cctctaaaat ttgcatgttt ctgtacagca ttatacataa
      841 ttttcttatt tccactgcat tcatttactc ttttttaaga ttgcacttcc tatgtaatta
      901 ttagataata tccaatcaca gtaaaatttt gatctaaaac aaaatgagcg atattcaaat
      961 tcctttaatc tttactgatg cggcggctcg taaagtaaaa agcttaattg agggcgaaga
     1021 gaacccgaat cttcgcttgc gtgtgtatat tacgggtggg ggctgtagtg gattccagta
     1081 tgggttcacc tttgatgatc aagtcaatga gggagacctt accattgaga atcaaagcgt
     1141 tggcttggtg gttgacccga tgagcctaca atatttaatt ggtggcacgg tggattatac
     1201 cgaagggctg gacggctcac gttttgtcgt gaataatcca aatgcaagct caacctgtgg
     1261 ttgtggttct tcctttagca tttaattcac tcgaattatt gccttattca atatcagtct
     1321 cacaagtaaa ttttttacta aaaatgaccg tttgtattaa acaaaatagc ctaacttatt
     1381 gagtttaggc tattttttat cattatgttt gcagttgcct ttacaacaaa gtgggacagg
     1441 atcttcgcct ccctcactca aagggttgca acgcagaata cgcttaaacg ctaaataccc
     1501 acctttaatt gctccgtgga ttttaatcgc ttcgaccgca taggtggagc aggttgggta
     1561 aaatcggcaa cgtgggccaa tcaatgggct gatagcatag cgatagaaat aaatcggtag
     1621 caatagaatt ttggctaata ggctgagacg aggtggctga tttatggatt ctgttgtgcc
     1681 ggctttttgc tcaggcgaat gtgtcttgac catagttttt ccaatgtttc aaacagcgtt
     1741 gcgttgtcta atttgccaat gccgcctttt gccacaaata caaaatcgaa attcggcaat
     1801 tcgtgttgct tgaggcgaaa actttcacgc actatccgct taatgcgatt acggtcgtga
     1861 gcccgtttta aatgtttttt ggcaacagtt aagcctagac gaggattttc tagattattc
     1921 tctctagcaa gaagagtcat ttggggtgtg ctggctcgaa gtggctcttc aaacaccgcc
     1981 ttaaattgaa cgggagctaa caaacgtagc tcccgagaga aatttagctt cttcacactt
     2041 aatcagaaag gtgttttggc tggttctgca attatgcaga taaagattta cgacctttag
     2101 cacgacgacg cgctaacact tgacgaccgt ttttagtagc catacgagca cggaaaccgt
     2161 gagtacgagc acgttttaat actgatggtt gaaatgtacg tttcattgct tatctaccta
     2221 aaatttaaaa agttggcata aaatggtttc tgccaaacaa ggaccataaa aaagagttgc
     2281 aattctacag gcaattaaaa taaaaagcaa gaaacaatcc aagcacaact cgggttgttt
     2341 gcaaaatttt tgaaaaatac gaccgcttgt tataatcgca gaaatttata ttggtttgtg
     2401 gcataatcga aaaaatatta actattgatg agaaatagaa caatgaatca accccaacaa
     2461 ataggtattt tgctggctaa tttaggtacg ccagatgaac ccacgccaag tgcggtgaag
     2521 cgttatttaa agcaattttt aagcgatcct cgtgtgatcg atctaccaaa atttaaatgg
     2581 caagcaatcc taaactgcat cattttgcca agacgttcgc caaaagtggc aaaattatat
     2641 caatcagtat ggagcgaaga aggctcaccg ttattggcga tttctcggca acaacaacga
     2701 gcggtacaac gctattttga tgccaaaggc aaaaatgtgg tggtggaatt gggaatgtct
     2761 tacggcaatc caagtattga aagtgcgacc gataacctca tcaagcaggg ggttgagcgg
     2821 attattgtgc taccgcttta tccgcaatac agtagcacta ctaccgcttc ggtgtttgat
     2881 gcgtttgcca acggtttaaa agcacagcgt aaaatcgtgc cgtttgattt tattcacagc
     2941 tatcataacg atccgcttta tattcaagcg ttggctaact ctattgatgt taaacctgac
     3001 gagttattac tgttttcatt tcacggcatt cctaaacgtt atgaaacgga aggtgatttt
     3061 tacgccaagc attgccaaga aacagcagaa ttagtggctg aaaagctagg tctgccaaga
     3121 gaaaaatggt atttgtcttt ccagtcccgt tttggtgatg aagagtggct acagccttat
     3181 accgatcaaa cccttgaaag cttcccacaa aaagggataa aaaaagtggc tgtcgtttgc
     3241 ccgggctttt cagcagattg tttggaaacc ttagaagaaa ttacagaaga aaaccgcgag
     3301 aactttgaac atgccggcgg agaatcttat cgctacattc cagcgttaaa tgcgagtggc
     3361 gatcatatca aggcattagt aaaattaatc gaagcgaaaa tataatataa tttgggcgga
     3421 taataaatcc gcccatt
//
LOCUS       DANLYI010000215         3412 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.217, whole genome shotgun sequence.
ACCESSION   DANLYI010000215 DANLYI010000000
VERSION     DANLYI010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3412
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.217"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            80..1882
                     /gene="frdA"
                     /locus_tag="PXD81_002264"
     CDS             80..1882
                     /gene="frdA"
                     /locus_tag="PXD81_002264"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010944100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HDL5538246.1"
                     /translation="MQSVNFDVAIIGAGGGGLRAAIAAAEANPNLKIALISKVYPMRS
                     HTVAAEGGSAAVIKDTDSFDNHFNDTVGGGDWLCEQDIVEYFVEHSPIEMTQLERWGC
                     PWSRREDGEVNVRRFGGMKIERTWFAADKTGFHILHTLFQTSIKYPNIVRFDEHFVLD
                     ILTDNGEARGCVAMNMMEGTLVQINANAVVIATGGGCRTYRFNTNGGIVTGDGLSMAY
                     RHGVALRDMEFVQYHPTGLPNTGILMTEGCRGEGGILVNKDGYRYLQDYGLGPETPIG
                     KPENKYMELGPRDKVSQAFWQEWRKGNTLKTAKGVDVVHLDLRHLGEKYLLERLPFIC
                     ELAKAYEGVDPAKAPIPVRPVVHYTMGGIEVDMNAETSIKGLFAVGECASSGLHGANR
                     LGSNSLAELVVFGKVAGENAARRALEVTARNQPQIDAQAKDVVARLHALANQEGNESW
                     SDIRNQMGDAMEEGCGIYRTQESMEATVNKIQELKERYKNISVKDKSSVFNTDLLYKI
                     ELGFILDVAQSIACSAVERKESRGAHQRLDYVERDDVNYLKHTQAYYNAEGTPTIKYS
                     DVKITKSQPAKRVYGAEAEAQEKVKKAAEAQAQQ"
     gene            1898..2629
                     /locus_tag="PXD81_002265"
     CDS             1898..2629
                     /locus_tag="PXD81_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005639269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HDL5538247.1"
                     /translation="MANLDKMTIEVLRYNPESDSEPHLDRYEVPYDSQTSLLDALGFI
                     KDELEPELSYRWSCRMAICGSCGMMVNGKPKLACKTFLRDYSGFMRIEPLANFPIERD
                     LVVDLSHFIDSIEAIKPYIIDNKAPEGQRTKQTPAQLEKYRQFSMCINCGLCYAACPQ
                     FGLNPEFIGPAAITLAHRYNLDNRDNGREERMKLLSSKNGVWSCTFVGYCSEVCPKHV
                     GPASAVNQGKLESAKDYVISMIKPR"
     gene            2639..3031
                     /gene="frdC"
                     /locus_tag="PXD81_002266"
     CDS             2639..3031
                     /gene="frdC"
                     /locus_tag="PXD81_002266"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HDL5538248.1"
                     /translation="MSATKRRPYVRGMKASWWKKLDFYKMYMVREATCLPTVWFCIVL
                     FYGAVALSKGTFTTDFVDFLQNPLVVILNIISLAAMLYHAATLFVMTPAVMSVMVKGK
                     HLPVSVGRNILWAATGVISVIALILVYI"
     gene            3045..3392
                     /gene="frdD"
                     /locus_tag="PXD81_002267"
     CDS             3045..3392
                     /gene="frdD"
                     /locus_tag="PXD81_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HDL5538249.1"
                     /translation="MSLDQNPKRSNEPPVWLLFSAGGMVSALFFPVVIFIIGLLLPFG
                     LVSPDNIISFAHTWIGKLAIMVLAIFPMWAGMHRIHHGLHDLKVHTPAGNVIFYGLSI
                     LYTILVIIAVVQI"
BASE COUNT          990 a          632 c          825 g          965 t
ORIGIN      
        1 aggttatctc attgctcaaa tagtggcaca atatgattac tttttaatga attaagttat
       61 ttaactttcg gagtgcatta tgcaaagtgt taattttgat gtcgcgatta taggtgccgg
      121 tggtggtggc ttgcgtgctg ctattgcggc ggctgaagca aatccaaatc taaaaattgc
      181 tttaatttca aaagtttacc caatgcgtag ccatacggtt gcagcagaag gtggttcagc
      241 ggcggtaatt aaagataccg actctttcga caatcacttt aatgataccg tcggtggtgg
      301 tgactggtta tgtgagcaag atattgtgga gtattttgta gagcattcgc cgattgaaat
      361 gacacaatta gaacgctggg gttgcccttg gagtcgtcgt gaagacggtg aagtgaatgt
      421 tcgccgtttt ggaggaatga aaattgagcg tacttggttt gcggcggata aaaccggttt
      481 ccacattttg catactttgt tccaaacttc gattaaatat ccgaatattg tgcgttttga
      541 tgagcatttt gtgctggata ttctcaccga caacggcgaa gctcgtggtt gtgtggcaat
      601 gaatatgatg gaaggcacac ttgttcaaat caatgcaaat gcagttgtga ttgcaaccgg
      661 tggcggctgc cgtacttatc gctttaacac taacggcggt attgtaaccg gcgacggttt
      721 atcaatggcg tatcgtcacg gtgtggcttt gcgtgatatg gagttcgtac aatatcaccc
      781 aacaggtttg ccaaataccg gtattttgat gacagaaggt tgtcgtggtg aaggtggtat
      841 tttagttaat aaagatggct accgttacct gcaagattat ggtttaggac cggaaacacc
      901 tatcggcaaa ccggaaaaca aatatatgga attaggccct cgtgataagg tttcacaagc
      961 attctggcaa gaatggcgta aagggaacac cttaaaaacc gcaaaaggcg tggacgtagt
     1021 tcacttagat ttacgccatt taggtgaaaa atatttactt gagcgtttac cgtttatttg
     1081 tgaattagcg aaagcctatg aaggggtcga tccggctaaa gcgccgattc ctgttcgccc
     1141 ggtggttcac tacacgatgg gtggtattga agtggatatg aatgcggaaa cctcaattaa
     1201 aggcttattc gcagtaggtg aatgtgcttc atcaggcttg cacggtgcga accgtttggg
     1261 ttctaactca ttagcagagc ttgtggtgtt cggcaaagtt gccggtgaaa atgcggctcg
     1321 ccgtgcgtta gaagtgactg ctcgcaacca gccacaaatt gatgcacaag caaaagatgt
     1381 ggtggctcgc ttgcacgcat tagcgaacca agagggtaat gaatcttggt cggatatccg
     1441 taaccaaatg ggtgatgcga tggaagaagg ttgtggtatt taccgtaccc aagaaagtat
     1501 ggaagcgact gtaaacaaaa tccaagagct aaaagagcgt tataaaaaca tcagcgtaaa
     1561 agataaatca agtgtgttta acactgattt actctacaaa attgaattag gcttcatttt
     1621 agatgtggct caatccattg cttgctctgc ggtagagcgt aaagaatctc gtggggctca
     1681 ccaacgttta gactatgttg agcgtgatga tgtgaactac ttaaaacaca cacaagcgta
     1741 ctacaatgca gagggtacac cgacgattaa atacagcgat gtgaaaatca ctaaatcaca
     1801 accggctaaa cgtgtgtatg gtgcagaggc tgaagcccaa gaaaaagtca aaaaagcagc
     1861 agaagctcaa gcacaacaat agagaaggag tagtaaaatg gcaaatttag ataaaatgac
     1921 catcgaagtg ctacgctaca accctgaatc agatagcgag ccacatttag acagatatga
     1981 agtgccgtat gacagccaaa cttcactttt agatgcattg ggcttcatta aagacgaatt
     2041 agaaccggaa ctttcttatc gctggtcttg ccgtatggcg atttgtggtt cttgcggaat
     2101 gatggtaaac ggtaagccga agttagcttg taaaacattc ctgcgtgatt acagcggttt
     2161 tatgcgaatt gagccgctgg cgaacttccc gattgagcgt gacttagtag tggatttaag
     2221 ccactttatt gatagcatcg aggcaattaa accttatatt attgataata aagcaccgga
     2281 aggacagcgt actaaacaaa cgccggcaca attagagaaa tatcgtcaat tctcaatgtg
     2341 tatcaactgc ggtttatgtt atgcggcttg cccacaattt ggtttgaacc ctgaatttat
     2401 cggacctgct gcgattacat tggctcaccg ctataacttg gataaccgtg ataatggtcg
     2461 tgaagagcgt atgaagctac tcagcagcaa aaacggtgtt tggagctgta ctttcgtggg
     2521 ttactgctcg gaagtctgtc cgaaacacgt cggccctgct tcggctgtaa accaaggtaa
     2581 acttgaaagt gcgaaagatt atgttatctc aatgatcaaa ccgagatagg aggaagaaat
     2641 gtcagcaaca aaacgtagac cttatgtaag gggaatgaaa gccagctggt ggaaaaaact
     2701 cgacttctat aaaatgtata tggtgcgtga agctacctgt cttccaactg tttggttctg
     2761 cattgtatta ttttatggag cagttgcgtt aagtaaagga acatttacta ccgattttgt
     2821 ggatttctta caaaatccgt tagttgttat cctaaacata atttcattgg cagcaatgtt
     2881 atatcatgcg gcaacacttt ttgtgatgac acctgccgtc atgtccgtga tggttaaagg
     2941 taaacactta ccagtctcgg ttggaagaaa tattctttgg gcagcaacag gcgtaattag
     3001 cgtaattgca ttaatcttgg tttatattta agaaggggaa gaaaatgagt cttgatcaaa
     3061 acccaaaacg ttcaaatgaa ccgccggtat ggttgctttt tagtgctggg ggaatggtaa
     3121 gtgcattatt tttccctgtg gtaatcttta ttatcggttt attactgcca ttcgggttag
     3181 tatcaccaga taatatcatt tcctttgccc atacttggat cggcaagctc gctattatgg
     3241 tattagcgat tttccctatg tgggcaggaa tgcaccgtat ccatcatgga ttacacgacc
     3301 tgaaagttca cacgccagca ggtaatgtga ttttctacgg attgtctatc ttatacacaa
     3361 ttctagtaat catcgcagtc gtgcagattt aaatgagaaa gaccatcttg ta
//
LOCUS       DANLYI010000216         3349 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.218, whole genome shotgun sequence.
ACCESSION   DANLYI010000216 DANLYI010000000
VERSION     DANLYI010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3349
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.218"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            17..1951
                     /gene="cpdB"
                     /locus_tag="PXD81_002268"
     CDS             17..1951
                     /gene="cpdB"
                     /locus_tag="PXD81_002268"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005614893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HDL5538250.1"
                     /translation="MLVLGTQPSAFAQTAKNKAGLRVIATTDIHGFLTDFDYYKDAPT
                     EKFGFTRTASLIKQARAEVKNSVLVDNGDLIQGNPISDYQSTKGAKEGKPEPSIMALN
                     AMQYEVGTLGNHEFNYGLSYLDNAIKQAKFPIVNANIVKPGTDEPFFTPYVIQQKEIV
                     DEKGNKHTIKIGYIGFVPPQVMIWDKANLEGKVEARDIVKTAQKYVPIVKKEGADIVV
                     ALAHTGPSDEPYKEGAENCAFYLADVKGIDAVIFGHSHRLFPNKEFANSPNADIAKGT
                     MKGVPESMAGYWGNNISVIDLSLAENDGKWGVVDGRAELRPIYNTEEKKATVENHPEI
                     VALLKETHEATRQFVSQPIGKATDNMYSYLALVQDDPTIQIVNQAQKAYVENVVKGLP
                     ELAGLPILSAGAPFKAGGRKNDPTGYTEVDKGELTFRNAADLYLYPNTLVVVKATGAE
                     LKEWLECSAGMFKQIDVASDKPQYLLDWTGFRTYNYDVIDGVSYQFDITQPARYDGEC
                     KLINPNSHRVVNLTFDGKPVDPKQEFLIATNNYRAYGAKFPGTGDKHIVFAAPDENRQ
                     VLANYITTESKAHGQVSPKADNNWRIAPISSNAKLDIRFETSPTDKAKAFIKENAQYP
                     MEYIENDETGFAVYRIDLTK"
     gene            complement(2024..>3349)
                     /locus_tag="PXD81_002269"
     CDS             complement(2024..>3349)
                     /locus_tag="PXD81_002269"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HDL5538251.1"
                     /translation="VTRVVSFGFQAFILKYLIHYFNDNFFSRSQHDVVSEYTQFISDT
                     LNIEDSGEHIAQLHQLGYLPIRIKAIPEGKSVAVKVPLMTIENTHSSFFWLTNYLETL
                     INVSLWQPITSASIAFAYRKALAEFSEQTCDDNSHLPFQAHDFSMRGMSSLESAESSG
                     AGHLTSFLGTDTIPAISFVTAYYGSQHLIGTSIPASEHSVMSSHGVDELPTFRYLMAK
                     YPNNMLSIVADTTDFWHNITVNLPLLKQEILARPESAKLVIRPDSGDFFTIICGNNSS
                     PVEHERKGLIECLWDIFGGSINQKGYKVLNPHIGAIYGDGVTYDKMIRILEGLTAKGF
                     ASSNIVFGVGAQTYQRNTRDTLGFAIKATSITINGVEKAIFKAPKTDNGLKKSQKGRV
                     KVISYDHYVDELTALDDFSDDLLEVVFENGKLVKSTNFDEIRQNIAQQF"
BASE COUNT         1108 a          646 c          685 g          910 t
ORIGIN      
        1 gcttggtgca actgcaatgc ttgtattggg gacacaaccg tctgcatttg cacaaacagc
       61 caaaaataaa gctggcttgc gtgtcatcgc cactacggat attcacggtt tcttaactga
      121 ttttgattat tacaaagatg ctccaacaga aaagtttggt tttacccgta cggcaagcct
      181 aattaaacaa gctagagcag aagttaaaaa cagtgtgtta gtcgataatg gtgacttaat
      241 tcaaggtaat cctatttctg actaccagtc aaccaaaggt gcaaaagagg gcaaacctga
      301 accttcgatt atggcattaa atgcgatgca atatgaagtc ggaacgctgg gaaatcatga
      361 atttaactac ggtttaagct atttagataa tgctattaag caagcaaaat tccctattgt
      421 caatgcaaac attgtgaagc cggggacaga tgaaccattc tttacacctt atgttattca
      481 acaaaaagaa attgtagatg aaaaagggaa taagcatacc attaagattg gttatatcgg
      541 ttttgtacca ccacaagtga tgatttggga taaagctaat cttgaaggaa aagttgaagc
      601 tcgagatatt gtgaagaccg cacaaaaata tgtgcctata gtaaaaaaag aaggggcaga
      661 tattgtcgtt gcattggcac ataccggccc ttcagacgaa ccctataaag aaggcgctga
      721 gaactgtgcg ttttatctag ctgatgtaaa aggtattgat gcagttattt tcgggcattc
      781 acatcgcctg ttcccaaata aagaatttgc aaattcacca aatgccgata ttgcaaaagg
      841 aacaatgaaa ggcgtaccgg aaagtatggc tggctactgg ggaaataaca tcagtgtgat
      901 tgatttaagc ttggccgaaa acgatggtaa atggggcgtt gtggacggta gagcagagtt
      961 gcgtccaatt tataatacgg aagagaaaaa agcaaccgtt gagaaccacc ctgaaatcgt
     1021 tgcattatta aaagaaaccc atgaagcaac ccgtcaattt gtgtctcaac ctatcggtaa
     1081 agcaactgat aatatgtata gttaccttgc gttagttcaa gatgacccta ccattcaaat
     1141 tgtgaaccaa gctcaaaaag cgtatgtaga aaatgtggta aaaggcttgc cggagcttgc
     1201 aggattacca attctaagtg ccggagcacc gtttaaagcc ggtggacgta aaaatgaccc
     1261 gacaggctat accgaagtcg ataaaggcga attaactttc cgcaatgcgg cagacttata
     1321 cctttaccca aataccttag tggtggttaa agcaacagga gcagaattaa aagagtggtt
     1381 agagtgcagt gcaggaatgt tcaaacaaat tgatgtcgct tcggataaac cgcaatattt
     1441 actggattgg acaggtttcc gcacctataa ctacgatgta attgacggtg tcagctatca
     1501 atttgatata acccaaccgg ctcgttatga cggcgagtgc aaattgatca accctaattc
     1561 gcaccgtgtg gtgaacttaa cctttgacgg caaaccggtt gatcctaaac aagaattctt
     1621 aattgccacc aacaattacc gtgcttatgg agcaaaattc ccgggaacag gcgataaaca
     1681 tattgtgttc gccgcaccgg acgaaaaccg ccaagtgtta gcgaattata ttaccaccga
     1741 aagtaaagct cacgggcagg taagtccgaa agcggataac aactggcgta ttgcaccaat
     1801 tagctcaaat gcaaaactgg atattcgttt tgaaacctct ccaacagata aagctaaagc
     1861 tttcattaag gaaaatgcac aatatccgat ggagtatatc gaaaacgatg aaaccggctt
     1921 cgcagtttac cgtattgatt taaccaaata atcagagcta aatacaagcg gtctaatttg
     1981 caaaaaaatt aacaaatttg accgcttgtt tcattccatt tttctaaaat tgttgtgcta
     2041 tattttgccg aatttcatca aaattggtcg atttgaccaa cttaccattt tcaaaaacca
     2101 cctctaaaag atcatctgaa aaatcatcca gtgcggttag ctcgtcaaca taatgatcat
     2161 aagagattac ttttactcgc cctttttgcg atttttttaa gccgttatcg gttttaggag
     2221 ctttgaaaat tgccttttcg acaccgttaa ttgtaattga agtcgctttg attgcaaaac
     2281 ccagcgtatc acgagtattg cgttgataag tttgagcccc aacgccaaac acaatattgc
     2341 tggaagcaaa accttttgcc gttaagccct ctaaaatccg tatcatttta tcataagtga
     2401 cgccgtcacc ataaattgcc ccaatatgcg gattaagcac tttataacct ttttggttaa
     2461 tactaccacc aaaaatatcc cacaaacact caatcagacc tttgcgttcg tgttcaacgg
     2521 gggaggaatt gttaccgcaa ataattgtaa aaaaatcacc gctatcaggg cggataacca
     2581 attttgctga ttccggtcga gctaaaattt cttgttttaa taatggcaaa ttaacggtga
     2641 tgttatgcca gaaatcggtt gtatctgcca caatagatag catattattt ggatattttg
     2701 ccattaaata acggaaagtc ggcagctcat caacaccgtg tgaactcatc accgaatgtt
     2761 ctgatgccgg aatagatgta ccgattaaat gttgagaacc ataataggct gtcacgaatg
     2821 aaattgcagg aatggtatct gtgcctaaaa atgaggttag atgtccggca ccggagcttt
     2881 ctgctgattc taaggaactc attccacgca tagagaaatc gtgtgcctgg aagggtagat
     2941 ggctattatc atcacaggtt tgctctgaaa attcggctag agccttacga taagcaaatg
     3001 caatggaagc tgatgtaata ggctgccata atgacacatt aatcagggtt tccaaataat
     3061 tagtcagcca aaaaaagctt gagtgagtgt tttcaattgt cattaaagga actttcactg
     3121 caacagattt tccttctgga atagctttaa ttctgatagg taagtaaccg agctgatgca
     3181 attgtgcaat atgttcgcca gagtcctcaa tattcaatgt gtcagaaata aattgagtat
     3241 attcagaaac aacgtcatgt tgtgagcgag aaaaaaaatt atcgttaaaa taatgaatca
     3301 agtattttaa aataaatgcc tgaaagccaa atgatacaac acgtgtaac
//
LOCUS       DANLYI010000217         3305 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.219, whole genome shotgun sequence.
ACCESSION   DANLYI010000217 DANLYI010000000
VERSION     DANLYI010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3305
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.219"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            <1..2031
                     /locus_tag="PXD81_002270"
     CDS             <1..2031
                     /locus_tag="PXD81_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein"
                     /protein_id="HDL5538252.1"
                     /translation="LPHCEIRRRPLVGKTFIAQKRPNQIIGWGLRPSAVGSRAYAQKH
                     QLPYIALEDGFLRSLGLGVKGYPPFSLVYDDIGIYYDTTCPSRLEKLLLEKPLQDQEQ
                     IKLAERALSQILTYGLSKYNHALDFSEIPSAKEKIVLVIDQTFGDMAVKYGQASEVDF
                     NKMLETAISENPEAQIWVKTHPDVLSGKKKGYLTDLPQYTDRVRIITEDVNPASLIKL
                     CEKVYCVTSQMGFEALLLGKKVVTFGVPWFAGWGVTDDRNEAVQLLVQQNRRKDRSLL
                     QLFYAAYFDYSRYINPNTGEKGSIFDVIDYLYKAKRLNQRLSGNLYCIGMSLWKQAVI
                     KPFFNLPQCRLHFVRSLNSLKKRPLADNAKILVWSNGKADVLQFAQQNNIPLLRMEDG
                     FIRSVGLGANLVAPLSLVVDDLGIYFNAENTSRLEQILLSAEHFSETDLSYAETLRQK
                     LVASHIGKYNVGGGDVKFNAQGKKIILVPGQVEDDASIKSGSPQIKRNLDLLKKVREL
                     NPSAYIIYKPHPDVVSGNRVGQIPTAETRRFADQMVDNANILDCILIADEVHTMTSLA
                     GFEALLRGKKVHCYGLPFYAGWGLTEDRLKSARRGKSLTLSQLIAATLIYYPLYVDPI
                     SRQFIDANQAIHILSQQKRNLTNQRIKRSWVSKQYGKLQHLYRAFKYASHTK"
     gene            2054..3286
                     /locus_tag="PXD81_002271"
     CDS             2054..3286
                     /locus_tag="PXD81_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis protein"
                     /protein_id="HDL5538253.1"
                     /translation="MEHYLDELIINSQRILLLQGPIGSFFTEFARWLESEQKEVFKLN
                     FNGGDEYFYPNNSPNTFPYYDSLDNFEAYLIQFCQTHQIDNILCFGDNRYYHKVAKNV
                     CLKLKLVFWAFEEGYFRPEYITLEKWGVNAFSPIPKNHTFFLPYAELPVVSEPEKVSK
                     GFRCMAGKAIPYYWNAYKKRKAYAKYQHHRFLNLKYYLNLWTRSGIKRICYWLHDKGF
                     AKRVEKGEFGEFFIVPLQVYDDTQVRIHSEQKSVERFLREVLNSFAKFAPAHLNLIIK
                     HHPMDRGFIDYKMVIYEFLEQYPQLNGRVFYIHDVPLPVFLRYGKGMVTLNSTSGLSA
                     LLHNMPVKTLGRANYDFEGLTDKQPLDTFWANPQPPNSEVFKGYRQFHLHKTHINGNF
                     YHKSILRYPYNWENEAHR"
BASE COUNT         1056 a          536 c          687 g         1026 t
ORIGIN      
        1 ttacctcatt gtgaaattag gcgaagacct cttgtgggaa aaacctttat tgctcaaaaa
       61 agaccgaacc aaatcatcgg ctgggggctt cgtccttcag cggtgggatc aagagcttat
      121 gcacaaaaac atcaacttcc ctatattgca ttagaagacg gctttttacg ctcactaggg
      181 ctgggtgtaa aaggctatcc gccattttct ttagtttatg atgatatagg tatttattac
      241 gataccacct gtccttctcg tttagaaaag ttattgcttg aaaaaccttt acaggatcaa
      301 gagcaaatca agcttgcaga gagagcatta tctcaaatct taacttacgg actttctaaa
      361 tataatcacg cactagattt tagtgaaatc ccctctgcca aagaaaaaat cgtgttagtt
      421 attgaccaaa cctttggtga tatggcggtg aaatatggtc aagcgagtga agtggatttc
      481 aataaaatgt tggaaacggc aatcagtgag aatccagagg ctcaaatttg ggtaaaaact
      541 caccccgatg tattaagtgg caagaagaaa ggctatttga ccgatttacc tcaatatact
      601 gatagggtca gaattattac tgaagatgtg aatccggcaa gtttgattaa attgtgtgag
      661 aaagtctatt gcgtaacttc gcaaatgggg tttgaggcat tattattagg taaaaaagtg
      721 gttacctttg gtgtaccgtg gtttgccggt tggggggtaa cagatgatcg taacgaagcg
      781 gttcaactat tagttcaaca aaatagacgg aaagatcgtt cattactgca acttttttat
      841 gctgcgtatt ttgattacag ccgttatatt aatccgaata ccggtgagaa aggctctatt
      901 tttgatgtca tcgattatct atataaagcc aaaagattaa atcagcgctt aagtgggaat
      961 ctatactgta ttggtatgtc gctatggaaa caggcggtaa ttaagccgtt ttttaatttg
     1021 ccacaatgtc gattacattt tgttcgttcg ctaaattctt taaaaaaacg accgcttgca
     1081 gataatgcaa aaattttagt ttggagcaat ggcaaagcgg atgtcttgca atttgcacag
     1141 cagaataata ttccattgtt aagaatggaa gatggattca ttcgctctgt cggactaggc
     1201 gctaatttag ttgcaccgct ttctttagta gtcgatgatt taggcattta ttttaatgca
     1261 gagaacactt cacgattaga acaaatttta ttgagtgcag agcatttttc tgaaactgat
     1321 ttgtcttatg ctgaaacatt aaggcagaaa ttggtggcta gccatattgg aaaatataat
     1381 gtgggcggtg gcgatgtaaa atttaatgct caaggtaaaa aaataattct tgtaccgggg
     1441 caggtagaag atgacgcttc gatcaaatca ggttctccac aaattaaaag aaatctggat
     1501 ttactgaaaa aagtgaggga attaaaccct agtgcctata ttatttataa gcctcaccct
     1561 gatgtggtaa gtggtaatcg agtcggtcag attcctacgg cagaaacacg caggtttgcg
     1621 gatcagatgg tggataatgc caatatttta gattgtattt taattgcaga tgaagtgcat
     1681 acaatgacat ctttagccgg tttcgaagca ttattaagag gtaaaaaagt gcattgctac
     1741 ggattacctt tttatgctgg ttggggattg acggaagata gattgaagtc ggcacgcaga
     1801 ggtaaatctt tgacattatc tcagttaatt gccgcaacat tgatatatta ccctctgtat
     1861 gttgatccta taagcaggca gtttattgat gccaatcaag cgattcatat attgtcacaa
     1921 cagaaacgaa acctaaccaa tcaaaggatt aaacgttctt gggtgagtaa acaatatgga
     1981 aaattacagc atctttatcg tgcgtttaaa tatgcttccc acactaaata agcgtagagg
     2041 gtaggaaatt aagatggaac attatttaga tgagctaatc atcaatagcc agagaatttt
     2101 attgttacaa ggcccgatag gctctttttt cactgagttt gcccgttggt tagaaagtga
     2161 gcaaaaagag gtcttcaagt taaattttaa tggcggagat gaatatttct atccaaataa
     2221 tagcccgaat acttttcctt actatgatag cttagataat tttgaagcct atttaattca
     2281 gttttgccaa actcatcaaa tagataatat cttatgtttt ggcgataatc gttattatca
     2341 taaagtggct aaaaacgttt gcttgaagtt aaagttggtc ttttgggcgt ttgaagaagg
     2401 gtactttagg ccagaatata ttacgttgga aaaatggggt gtaaatgctt tttctccaat
     2461 ccctaaaaat cacactttct ttttgcctta tgccgagtta ccagtagtca gtgaaccaga
     2521 aaaagtatct aaaggcttta gatgtatggc gggaaaagca atcccgtact attggaatgc
     2581 ctataaaaaa cgtaaagcat acgcaaaata tcagcatcat cgttttctga atctcaaata
     2641 ctaccttaat ttatggacaa ggtcgggaat taagcgaata tgttattggt tacatgataa
     2701 aggctttgcc aagcgggtag aaaaaggaga gtttggtgaa tttttcattg tgcctctaca
     2761 agtttatgat gacactcaag tgaggattca tagtgaacaa aaaagtgtag agcgtttttt
     2821 aagggaagtt ctgaattcct ttgccaaatt tgcaccggca catctcaatt taattattaa
     2881 gcatcacccg atggatagag gatttataga ttataaaatg gtgatttatg aattcttaga
     2941 gcaatatccc caattaaacg gtagagtttt ttatattcac gatgtgccgt tacctgtctt
     3001 tttacgttat gggaaaggaa tggtgacttt aaattcaacc agtgggcttt ctgcattgct
     3061 ccataatatg ccggtcaaaa ccttaggtag agccaattat gattttgagg gattaaccga
     3121 taaacagcca ttagatactt tttgggctaa tcctcagcca cctaattcgg aagtatttaa
     3181 aggctacaga caatttcatt tacataaaac gcatattaac ggtaattttt accataaatc
     3241 gattttgcgt tatccttata attgggaaaa tgaagcacac aggtgatgga aaatattaaa
     3301 ataag
//
LOCUS       DANLYI010000218         3260 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.220, whole genome shotgun sequence.
ACCESSION   DANLYI010000218 DANLYI010000000
VERSION     DANLYI010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3260
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.220"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            complement(<1..595)
                     /locus_tag="PXD81_002272"
     CDS             complement(<1..595)
                     /locus_tag="PXD81_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDL5538254.1"
                     /translation="MQERITQQNERLSAYMDGQNVDTAFVETLTNSPELQQKWASYHT
                     IRSVMQGDEIILGRDFSAKMEALLENEEIESQANVEKPKGLLLKLKRWGTPIMQAGIA
                     ASVCLVAVLGVNSMNASDEIAQTQQPVLQTLPFSNSVEAVSYNAPVQDQPTEEQLELQ
                     QRKINALLENHELQRRTNNVKNLTLSEEEKQKAQTSSN"
     gene            complement(641..1213)
                     /gene="rpoE"
                     /locus_tag="PXD81_002273"
     CDS             complement(641..1213)
                     /gene="rpoE"
                     /locus_tag="PXD81_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000003307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HDL5538255.1"
                     /translation="MSEQITDQELVEKAQKGDKKAFNLLVVRYQNRVAGLLTRYVARD
                     DIPDIVQESFIKAYRSLESFRGESAFYTWLYRIAVNTAKNHLTSLGRRPPKEDILAED
                     AESYDVGAQLRESDTPENLILSEELKKVVFDTIENLPDELKTAIRLRELEGLSYEEIA
                     EVMECPVGTVRSRIFRAREAIDAKIKPLMQ"
     gene            complement(1435..2979)
                     /gene="leuA"
                     /locus_tag="PXD81_002274"
     CDS             complement(1435..2979)
                     /gene="leuA"
                     /locus_tag="PXD81_002274"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HDL5538256.1"
                     /translation="MSNNNIIIFDTTLRDGEQSLKASLTVKEKLQIALALERLGVDVM
                     EVGFPVSSAGDFESVQTIARHIKNSRVAALSRAVAKDIDAAYEALKVAEAFRIHTFIA
                     SSALHVEAKLKRSFDDVVEMAVAAVKRARNYTDDVEFSCEDAGRTGIDNICRIVEAAI
                     NAGATTVNIPDTVGFCLPNEYGNIIHQVMNRVPNIDKAIVSVHCHNDLGMATANSLTA
                     VQNGARQIECTINGIGERAGNTALEEVVMAIKTRQEFMGVDTRINTQEIHRVSQMVSQ
                     LCNMPIQPNKAIVGSNAFAHSSGIHQDGMLKNKNTYEIMSPETIGLKKEKLNLTARSG
                     RAAVKGHMADMGYTETDYDLDKLYEAFLQLADKKGQVFDYDLEALAFIDMQQGDEDRL
                     KLDVITTQLISGLPASAFVQVELDGVRQSATSQGGNGPVEATYNAIMNIVGMELKMSH
                     YNLTAKGEGAEALGQVDIVVEYEGRKFHGVGLATDIVESSARALIHAINAIYRSQKVA
                     DLKAKK"
BASE COUNT          886 a          719 c          580 g         1075 t
ORIGIN      
        1 tattggaaga agtttgtgct ttttgctttt cttcctcaga taaggttaaa tttttaacgt
       61 tattagtacg gcgttgtaac tcatggtttt ctaataacgc attaatctta cgctgttgaa
      121 gctctaactg ttcttctgtt ggttgatctt gaactggcgc attataactt accgcttcaa
      181 ctgaattgct aaatggcaat gtttgtaata ctggttgttg cgtttgagct atttcatcac
      241 tcgcattcat tgaattaacc cctaataccg caactaagca cactgaagct gcaataccgg
      301 cttgcataat tggagttccc caacgcttga gcttgagcaa taagccttta ggtttctcta
      361 catttgcttg agattcaatt tcttcatttt ccagtaatgc ttccatttta gcagagaaat
      421 ctctccctaa aatgatctca tcaccttgca tcacactacg aatagtgtga taacttgccc
      481 atttttgctg taattcaggg ctgttagtta atgtttctac aaatgctgta tcaacatttt
      541 gcccatccat ataagctgaa aggcgttcat tttgttgagt gatgcgttct tgcataattg
      601 actccaatga tttaaatctc ttctaaaata atctttctat ctattgcata agtggcttga
      661 ttttagcatc aatcgcttcc cttgctcgga aaatacggga acgtacagta ccaaccggac
      721 attccatcac ttctgcgatt tcttcgtaac ttagcccttc caattctctt aaacgaatgg
      781 cggttttaag ctcatcaggt aaattttcaa tcgtatcaaa cacgactttc tttaattcct
      841 cggataatat caaattctct ggcgtatcac tttcacgaag ttgcgcgcct acatcataac
      901 tttcagcgtc ttcggcaaga atatcttctt ttggcggtct tctgcctaaa gaggttaaat
      961 gatttttcgc agtattcact gcaatgcgat aaagccacgt ataaaaagca ctttcgccac
     1021 ggaaagactc taaagaacga taggctttaa taaaagattc ttgcacaatg tccggaatat
     1081 catctcttgc aacataacga gtcagcaaac cggctactct attttgataa cgtacaacaa
     1141 gtaaattaaa tgctttttta tcaccttttt gtgctttttc gactaattct tggtcggtga
     1201 tctgttcgct caactgcgga ctctcctaaa tatcaatatg tcattttcca ttgcacactt
     1261 tgttagtgtg tgctttatga caaaagttca aaaaatttga aaaattgtgt aattttttac
     1321 attcctagat aaaacctact taataaacta agcaagcggt cggattttag cgaaattttg
     1381 caaaaatttc aaaaaatccg accgcttgtc tatgttatgc tttcgctcta taagttattt
     1441 cttcgctttt aaatctgcca ctttttgtga acggtaaatc gcattaatgg cgtgaattaa
     1501 agctcgggct gaagattcca caatatcggt tgccaaaccc acaccgtgga attttcgacc
     1561 ttcatattcc actacaatat ccacttgccc taatgcttct gctccttcgc ctttcgcagt
     1621 cagattatag tgcgacattt ttagctccat tcccacaatg ttcataatgg cattgtaagt
     1681 tgcctcaact ggaccatttc ccccttgaga tgtagcactt tgacgaacgc cgtctaattc
     1741 cacctgtaca aaagccgaag ccggtaaacc gctgattagt tgggtggtaa ttacatctaa
     1801 ttttaagcgg tcttcatcgc cttgctgcat atcaatgaag gcaagagctt ctaagtcata
     1861 atcaaacact tgcccttttt tatcagcaag ttgcaagaag gcctcataaa gtttatctaa
     1921 atcgtaatcc gtttccgtat aacccatatc agccatatgc cctttcaccg ctgctctacc
     1981 tgaacgggca gttaaattca gcttctcttt tttcaagccg atggtttccg gcgacataat
     2041 ttcataagta tttttatttt tcagcatacc gtcttggtga atacctgaag agtgagcaaa
     2101 ggcattgcta ccgacaatcg ctttgttcgg ctgaatcggc atattgcaaa gctggctgac
     2161 catttggctg acacggtgaa tttcttgggt attaatgcgg gtatcaaccc ccataaattc
     2221 ttggcgggtt ttaattgcca ttaccacttc ttcaagagcg gtgttaccgg cacgttcgcc
     2281 aataccgttg atagtacact caatctgtct cgctccgttt tgcaccgctg tcaatgagtt
     2341 ggcggtagcc atacctaaat cgttatggca gtgaacagag acaatcgctt tatcgatatt
     2401 cggaacacgg ttcatcactt ggtggataat attgccgtat tcattcggta aacagaaacc
     2461 gactgtatcc ggaatattca ctgtggttgc accggcatta atggcagctt ctacaatgcg
     2521 gcagatgtta tcaattccag tacgcccagc atcttcacag gaaaactcaa catcatcagt
     2581 ataatttctg gcgcgtttta ccgctgcaac cgccatttcg actacatcat caaatgaacg
     2641 ttttaattta gcctcaacgt gtaatgccga gctggcaata aaggtatgga tacggaatgc
     2701 ttctgctact tttaaggctt cataagctgc atcaatgtct tttgcaaccg ctcgggaaag
     2761 cgccgccacc cggctgtttt tgatatgacg agcgatggtt tgtactgatt caaaatcgcc
     2821 cgctgaagaa actggaaaac cgacttccat cacatcaacc cctaaacgtt ctaaagcaag
     2881 ggcaatttgc aatttttctt ttacggttaa acttgctttt aatgattgct caccatcacg
     2941 taaagtcgta tcaaaaataa taatgttatt gttgctcata gaagcctctt tttaggtgtt
     3001 tttatttgat aaaaaaagcc cgcattttaa ggtgcgggca agaatgtgga tagtccattt
     3061 actttttcca atcaatcttt atcctcgcac aaaatacgat aacagagata atcggagtaa
     3121 ggtaaatttt tccatgttgt gttgtatgtt tatgtttgca ttgttattca gtagttgagg
     3181 tgtatattat ccttatccaa atcaatgtca atagcatttt tctcttcacg caaccgttta
     3241 ccagcccatt aattcaagtc
//
LOCUS       DANLYI010000219         3215 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.221, whole genome shotgun sequence.
ACCESSION   DANLYI010000219 DANLYI010000000
VERSION     DANLYI010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3215
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.221"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            32..178
                     /locus_tag="PXD81_002275"
     CDS             32..178
                     /locus_tag="PXD81_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5538257.1"
                     /translation="MDTTQKRVALFIIGLSVVMWLRKHNCCTVIFVLLLAALAFSYTM
                     EHLY"
     gene            194..400
                     /locus_tag="PXD81_002276"
     CDS             194..400
                     /locus_tag="PXD81_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5538258.1"
                     /translation="MVKNVVHEEILHNRAELQQAVEKEEFCAKCDNSVVFHLRDNYHE
                     FTLGLDTILQCLHFAEEKGAIRIP"
     gene            397..1173
                     /locus_tag="PXD81_002277"
     CDS             397..1173
                     /locus_tag="PXD81_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5538259.1"
                     /translation="MNIEYSLNHCQGDGVAFYGRLGTDDLIQLFNHLNPQKRKQKMFA
                     NLLTHIESWETHDDIGFEIIRNRFGYRYSHWNTMELYAKTADQLVFFNDSDARRNWYF
                     PTSKVYKYKGLWDEFIQDLEQYIKGTSREMESVGYQIIEASPYQTETVFKFNTENYLV
                     ELKRKPSEFYSELVDWAFGDMCDFEATIEQICQGQLCYADFEATVTDKKSEIQLGLAR
                     LSGISYSPNDRSFSGYRQELIGEAIASARHNAARFAYLHA"
     gene            1288..1700
                     /locus_tag="PXD81_002278"
                     /pseudo
     CDS             1288..1700
                     /locus_tag="PXD81_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484619.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemophilus-specific protein"
     gene            1714..1869
                     /locus_tag="PXD81_002279"
     CDS             1714..1869
                     /locus_tag="PXD81_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5538260.1"
                     /translation="METRIVKVIYDDGDYFFTRINADEQAIRNYYAIGSSIGFDDIRK
                     IIAVEIL"
     gene            1949..2923
                     /locus_tag="PXD81_002280"
     CDS             1949..2923
                     /locus_tag="PXD81_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012340807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1738 domain-containing protein"
                     /protein_id="HDL5538261.1"
                     /translation="MNTQPKFDLYQHITDRIVVALEQGTAPWLKPWSHPECNMALPRN
                     AVSNRLYSGINTLLLWIATAEHGYKQCKWITAKKANELGGYIRKGEKATVVMNYRPDE
                     REKLDEEGNIVYDEEGNPEMERHFFINKHNLFNLDQCENLPVELYDPVSEPKQPKSEP
                     NQYDIFAEIRQIIKGMDLQVEMKPSNRAFYQPGEDKVVMPEMKQFANAQAFHSVLLHE
                     MTHATGANNRLNREGITSGKAKFGNKVYAFEELIAEMGGAFLCAYLGFDTVPQNAAYI
                     ESWIRVLKEDKRAIVRASGKAREACQYMLDALNVAQLADKYDVFEEAA"
     gene            3010..3207
                     /locus_tag="PXD81_002281"
     CDS             3010..3207
                     /locus_tag="PXD81_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5538262.1"
                     /translation="MTKSTEIPRGHDNWVITGRDNDGKWHKERFLYYSRREAIQQFRR
                     KHNCVGKHSVIESITPQFGWY"
BASE COUNT         1005 a          558 c          710 g          942 t
ORIGIN      
        1 aaaatggcag ccttacaaga aagaatgaca gatggatacg acacaaaaac gtgtggcttt
       61 attcatcatt ggattatctg ttgtgatgtg gctaagaaag cataactgct gtacagtgat
      121 atttgtattg ttgttggcag cgttagcatt tagctacacg atggaacatt tatattagct
      181 ggggaactga attatggtaa agaatgtggt tcatgaagag atattacata accgtgcaga
      241 attacagcaa gcggttgaaa aagaagaatt ttgtgcaaaa tgtgataact cagtagtatt
      301 ccatttacgt gataattatc acgaatttac gcttgggtta gatactatat tgcagtgtct
      361 gcattttgct gaagagaagg gagctatccg gataccttga atattgagta ttcactcaat
      421 cattgccaag gagatggagt cgcattttat ggtcgacttg gtacagatga tctaatccag
      481 cttttcaatc atctcaatcc acagaaacgt aaacagaaaa tgtttgctaa tttgctaacg
      541 cacattgagt cttgggaaac ccacgatgat attggttttg aaattatacg aaatcgcttt
      601 gggtatcgtt actcgcattg gaatacgatg gagctttatg ctaaaactgc agatcagtta
      661 gtgtttttca atgatagtga tgctcgaaga aactggtatt ttccgacttc aaaagtgtat
      721 aaatacaaag gcttgtggga tgagtttatt caggatttag aacagtatat taagggtact
      781 tcccgtgaaa tggaatcggt cggttatcaa attattgaag cctcgccgta tcaaacggaa
      841 accgttttca aatttaatac ggaaaactat ctagtagaac tgaaacgtaa accttctgag
      901 ttttattcag agcttgttga ttgggcattt ggtgatatgt gtgattttga agcaactata
      961 gagcagattt gtcaagggca actctgttat gctgattttg aagcaactgt taccgataag
     1021 aaaagtgaaa ttcagttagg tcttgctcgc ttgagtggaa taagttactc gccgaatgac
     1081 cgttcgttta gtggttatcg acaagagctt attggtgagg cgattgcctc tgctcgccat
     1141 aatgcagcac gttttgccta cttgcacgcc taatttgcag atttgtactt caatttgacc
     1201 gcttgtagtg gttaattttc accccgtggg gaaatatttt ccctattggg caagatgttt
     1261 ctccactttt taatttggag aaatgtgatg aaaaatgaac acaatttaga gcaacttaaa
     1321 tatatttggg ataaccgcaa ccttgaaaat aaagcctatc aatacctgtt tcacatttgc
     1381 attgagccga tgaaaaaagg agattggaat cgttatacga tttggcttaa ttttcacggc
     1441 aacttaatca acgtgtcaga cgttgtatat cgagttgtgg aatctcaaaa gctcaaggat
     1501 gacatcaaaa tgtggtatga caataatacc attgtcgtcc gataaaagtt gaaacaacag
     1561 gcttcatctc atatttactg caccagttct atcaggggat agcctatatt ttactggaac
     1621 agggaatgat tactgaaact gaagcagccg agaaaagtta taacttcaga gctgcaaatt
     1681 gttttcaacg tttgctttaa tggaggtgag ataatggaaa ctcgcattgt taaagttatt
     1741 tacgatgatg gtgattattt ctttacccga atcaatgctg atgaacaggc tatccgtaat
     1801 tactacgcca taggcagttc aataggtttt gatgatataa gaaaaattat cgctgttgaa
     1861 attttataaa ccacccatag gggaaactat ttccccatag ggtgatagcg tttccccatt
     1921 tttctacccg aactttggag aaacacttat gaacactcaa cctaaatttg atttatacca
     1981 acacatcact gaccgaattg ttgtagcact ggaacaaggt acagccccgt ggttaaaacc
     2041 gtggagccat ccggaatgca atatggcatt acctcgcaat gcggtatcta accgcttgta
     2101 ttccggtatc aatacactct tactctggat tgctactgct gaacatggtt acaaacagtg
     2161 caagtggatt acagcaaaaa aagccaatga actcggcgga tatatccgta aaggggaaaa
     2221 agctactgtg gtgatgaact accgtcctga tgagagagag aaactcgatg aggaagggaa
     2281 tattgtgtat gacgaggaag gaaatccgga aatggaacga cactttttca ttaataagca
     2341 caatctgttc aatctggacc agtgtgaaaa tttaccggtg gaactgtacg atcctgtatc
     2401 agaaccgaaa caacctaaat ctgagccaaa ccaatacgat atttttgctg agattcggca
     2461 gattatcaaa ggaatggatt tgcaggtgga aatgaagcct agcaatcggg cattttatca
     2521 accaggtgaa gataaggtcg taatgccgga gatgaagcag tttgcaaatg ctcaagcgtt
     2581 tcatagtgta ttgttacacg aaatgactca cgcaacgggg gcaaataatc ggcttaatcg
     2641 ggaaggtatt acttccggaa aggctaaatt tggaaacaaa gtctatgctt ttgaggagtt
     2701 gattgctgaa atgggcggtg catttttatg tgcctacctt ggttttgata ctgtgcctca
     2761 aaatgcggcg tatattgaaa gttggattcg agtattgaaa gaagataaac gggcgattgt
     2821 ccgtgcttcc ggaaaagctc gtgaagcctg ccaatacatg ttagacgcat taaacgtcgc
     2881 acagttggct gacaaatacg atgtatttga agaagctgct taacaaattt gccgaaaggc
     2941 atatctcaag gggagaccta tccctttggg catagtgttt ccctattttt tattaaatgg
     3001 agaaaagcta tgacaaaatc aactgaaata cctcgtggac atgataactg ggttattaca
     3061 ggcagagaca atgatgggaa atggcataaa gaacgttttc tttattattc tcgccgtgaa
     3121 gccatccaac agtttcgtcg aaaacacaac tgcgtaggta aacatagtgt tattgaatca
     3181 ataacacctc aatttggctg gtattaaccg gcttt
//
LOCUS       DANLYI010000220         3196 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.222, whole genome shotgun sequence.
ACCESSION   DANLYI010000220 DANLYI010000000
VERSION     DANLYI010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3196)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3196)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3196
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.222"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            6..842
                     /locus_tag="PXD81_002282"
     CDS             6..842
                     /locus_tag="PXD81_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012621753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDL5538263.1"
                     /translation="MLFACINTLIPKLTSISPIDSNVIALVQYLAAFIFLVPSMNNIG
                     FFSSLKTEHFGQHCFRIFLSAIGIQCWTMALANQLPIWQGIALLMTSPLFVTIGSGLF
                     LKEKVDKKRWIATAMGFVGAMIILEPWSDHFEMIALLPIAAAFFWAGYSLMVKKLSRY
                     DSPTTMVAYLFILITPFNLLIALTNLSPSGFSLPSWSDFGFLILLGFFTALAQLAVAK
                     AYSVADASYIQPFDFIKLPLNVLAGWLVFNWVPPGKLWLGAAIIIAATIYITHAETKQ
                     SN"
     gene            complement(921..1412)
                     /gene="ilvN"
                     /locus_tag="PXD81_002283"
     CDS             complement(921..1412)
                     /gene="ilvN"
                     /locus_tag="PXD81_002283"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005647229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HDL5538264.1"
                     /translation="MRRTLAVLLENESGALSRVVALFSQRGFNIESLTVAPTDDPSLS
                     RMTIVAQGEEQVLEQIEKQLHKLIDVFKVSNLSAYEHVEREVLLVKVRATGSSRDELK
                     RMVDIYRGQIVDLTPKLYTIQLSGSTEKLNAFIEAVKEETTIVEIVRSGVISLSRGDK
                     NAL"
     gene            complement(1405..3126)
                     /locus_tag="PXD81_002284"
     CDS             complement(1405..3126)
                     /locus_tag="PXD81_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HDL5538265.1"
                     /translation="MKKLSGAEMIVQSLKDEGVEYVFGYPGGSVLDIYDAIHTLGLNH
                     VLVRHEQGAVHMADGYARSTGKVGCVLVTSGPGATNTITGILTAYTDSVPLVILSGQV
                     PSSLIGTDAFQESDMIGISRPVVKHSFLVKNTEEIPEIIKKAFYIASTGRPGPVVIDI
                     PKDVVNPLNKYPYEYPQEVSMRSYNPTVQGHKGQIKKALKALLVAKRPVLYVGGGVIS
                     AEGSPEITEFAQKLNLPVTCSLMGLGAYPASDKQYLGMLGMHGTFEANNAMHESDLIL
                     GIGVRFDDRTTNNLAKYCPNAKVIHVDIDPASISKTVQAYIPIVGSAKDVMEEFLSLL
                     GEENLAKNQVDLTAWWQQIDAWKARNCLAFEENSDVIKPQQVIRMIHKLTNGEAYVAS
                     DVGQHQMFAALHYGFDKPRRWINSGGAGTMGFGLPAAMGVKFAHPDATVVCVTGDGSI
                     QMNIQELSTAKQYDTPIVIVSLNNRFLGMVKQWQDIIYSGRHSQVYMNSLPNFAKLAE
                     AYGHVGIQIDHPSELEAKLTEAFSIKDKLVFVDVLVDETEHVYPMQVRGGAMNEMMLS
                     KTERTDA"
BASE COUNT          881 a          693 c          600 g         1022 t
ORIGIN      
        1 ccggtgtgtt atttgcctgt atcaatacct taattcccaa attaacgtcc atttcaccga
       61 ttgactcgaa cgtgattgca ttggtgcaat atttagctgc ctttattttc ctcgttccca
      121 gcatgaataa tatcggtttc ttctcttcat taaaaaccga gcattttggg caacactgtt
      181 tccgtatttt tctatctgcg attggtattc aatgttggac aatggcactc gctaaccaat
      241 tacctatttg gcagggaatt gcgttattga tgacttctcc attgtttgtg accatcggct
      301 ccggtctgtt tttgaaagag aaagtggata aaaagcgttg gattgcaacc gcaatggggt
      361 ttgttggggc gatgattatt ctagagcctt ggagcgatca ttttgaaatg attgcgttac
      421 tgccgattgc cgcggctttt ttctgggcag gttattcgct aatggtgaaa aaactctctc
      481 gttacgatag cccaactacg atggtcgctt acctgtttat tctaattacg ccttttaatt
      541 tgttgattgc actcacgaac cttagcccta gtgggtttag tctgccttct tggagcgatt
      601 tcggcttttt aatcttactt ggtttcttta cggcgttagc gcaactggcg gtagccaaag
      661 cctacagtgt agcagatgct tcctacattc agccgtttga ttttattaaa ttacctttga
      721 acgtattagc cggttggctt gtatttaact gggtgccacc gggtaaatta tggctaggag
      781 ctgcgattat tattgctgcg accatttata ttactcacgc tgaaactaaa cagagtaatt
      841 agcaacaaat tcacgctaca taaaaatccc tttatctcaa agataaaggg atttgcaaat
      901 tttaagctaa aattgaccgc ttacagagcg tttttatcgc cacgagatag actaatcacg
      961 ccagagcgaa caatctctac aatcgttgtt tcttctttaa ctgcctcaat aaatgcattg
     1021 agcttctcgg tagaaccgga taattgaatg gtgtaaagtt tcggtgttaa atctacgatt
     1081 tgcccacggt aaatatctac catacgtttc agttcatcac gagatgaacc tgtagctcgc
     1141 acttttacca ataacacttc acgctcaacg tgttcataag cacttaagtt actcacttta
     1201 aacacatcaa tgagtttgtg tagttgcttt tcgatttgtt ctaacacctg ttcttcgcct
     1261 tgcgcgacaa ttgtcatacg ggaaaggctt ggatcatcag taggggcaac ggtcaagctt
     1321 tcaatgttaa aaccacgctg cgagaaaagg gcaactacac gagataatgc acctgattca
     1381 ttttctaata atacggctaa tgttctacgc atctgttctc tccgttttac ttaacatcat
     1441 ttcattcatt gctcctccac gcacttgcat tgggtaaaca tgttccgttt catcgaccaa
     1501 tacatcaaca aatacaagct tatctttaat tgagaatgcc tcagttaatt ttgcctccaa
     1561 ttcagaaggg tggtcgattt gaatcccaac gtggccataa gcctctgcta atttagcaaa
     1621 gttaggtaga gaattcatat acacctgtga atggcggcca gagtaaataa tatcttgcca
     1681 ctgtttcacc atacctaaga aacggttatt taagctgaca atcacaatcg gtgtgtcgta
     1741 ttgctttgcg gtggaaagtt cctggatatt catttggata ctaccatcac cggttacaca
     1801 aacaacagtg gcatcagggt gggcaaattt tacccccatc gcagcaggta agccaaagcc
     1861 cattgtgcct gcaccgcctg aattaatcca acgacgtggt ttatcgaacc cataatgcaa
     1921 tgcagcaaac atttgatgtt gtccaacatc tgaggcaaca taagcctccc cattggtcaa
     1981 cttgtggatc attctaatca cttgttgtgg cttaatcaca tcactgtttt cttcaaaggc
     2041 taagcaatta cgagctttcc aagcatcaat ttgttgccac caagcggtta aatctacctg
     2101 attttttgca agattttcct cacctaataa agataaaaat tcttccatta catccttagc
     2161 actgcctaca attggaatat aagcttgtac tgttttggaa attgaggctg gatcaatatc
     2221 cacgtggatc acttttgcat tcgggcaata ttttgctaag ttatttgttg tacgatcatc
     2281 aaaacgtacc ccaataccta aaatcaaatc actttcgtgc attgcattat ttgcctcaaa
     2341 agtgccgtgc atgcctaaca tacctaaata ttgcttatca gaggcaggat aagcacctaa
     2401 tcccattaat gaacaggtta caggtagatt tagcttttgt gcaaactctg taatttctgg
     2461 cgaaccctct gcactaatta caccaccgcc tacataaagc actggacgtt ttgctactaa
     2521 tagtgctttt aatgcttttt taatttgccc tttatgccct tgaactgttg ggttataaga
     2581 acgcattgat acttcttgcg gatattcata gggatattta tttaatggat tgactacatc
     2641 tttaggaata tcaataacta ccgggcctgg tcgaccggtt gatgcaatat aaaaggcttt
     2701 tttgataatt tcaggaattt cttctgtatt ttttactaag aaactgtgtt ttacaaccgg
     2761 acgagaaatg ccgatcatat cactttcttg gaatgcatct gtcccaatta aacttgatgg
     2821 aacttggccc gacagtatta ctaacggaac agagtcggta taagcagtta aaataccagt
     2881 aattgtattc gttgctcccg gacctgaagt cactagcaca cagccgactt tacctgttga
     2941 acgggcatag ccatcagcca tatgtacagc accttgctcg tgtcgaacta aaacgtgatt
     3001 caatccaagt gtatgaattg catcataaat atcgagtaca gagccacccg gatagccgaa
     3061 aacgtattct acgccttcat cctttaagga ctgtacaatc atttctgcac cagaaagttt
     3121 tttcattgaa tcctccaaca aaatagcatt ttcttctcta agatggttgg attttgcagt
     3181 aaaattttat tgatgt
//
LOCUS       DANLYI010000221         3161 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.223, whole genome shotgun sequence.
ACCESSION   DANLYI010000221 DANLYI010000000
VERSION     DANLYI010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3161)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3161)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3161
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.223"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            <1..524
                     /gene="proQ"
                     /locus_tag="PXD81_002285"
     CDS             <1..524
                     /gene="proQ"
                     /locus_tag="PXD81_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HDL5538266.1"
                     /translation="EQKVEQTQNKTNPSTKEVIAYLAEKFPLCFTLEGEVKPLKVGLF
                     QELAEALKDDEKISKTLLRQVLRNYTNSWRYLAASQANVARVGLQGEEVGVVTEDEAT
                     HAAEALAAAKAVVAERKAQERKAQRKEYFKQKAREENAKKRAEFKAKKAIDNLPKASE
                     EKLAALADKFGRK"
     gene            610..2622
                     /gene="prc"
                     /locus_tag="PXD81_002286"
     CDS             610..2622
                     /gene="prc"
                     /locus_tag="PXD81_002286"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HDL5538267.1"
                     /translation="MKLTRLNKIFALALGLMIGNAFAVQPDIKESAIVMPKPNEQHSL
                     STKRVTARLTQSHYHKFQLNDEFAGKIFNRYVDWLDPSHNTFLQADIDEMRSKYATTL
                     DDDLYAGKLDAAFAMYELMSKRRYERYSYALSLLDKEPDLKGNDEIEIDREKAAFPKS
                     VEEANTLWEQRVKNDIINLYLKDKKWPEIKKTLTKRYNFAIKRLTQVKADDILQTYLN
                     SFSREIDPHTSYLSPRAAKAFQESMNLSLEGIGATLTQEDDVTSIRALVPGAPAAKSK
                     KLVVGDKIVGVGQGERGEIEDVIGWRLDNVVDKIKGKKGTKVRLEIEPEKGGKTKIIT
                     LVRDKVRIEDSAAKLTVEKVDGKNVGVIKIPTFYIGLTSDVRKLLTQIEKKNVEGLII
                     DLRENGGGSLTEVIELTGLFIKDGPVVQVQDAFNRIKVHEDPESKSVYDGKVMVMINR
                     HSASASEIFAAAMQDYNRAIIVGQQTFGKGTVQQSRSLNFVYDLDQEPLGFIQYTIQK
                     FYRINGGSTQLKGVEADIKFPEIINAAKTGESFEDNALPWDKIPAANYKEAGNARNAV
                     AALTAKHQERIAKNPEFIVLNENIAIRKERDERKFLSLNLEERLKENKADESKRLKDI
                     NARFAREGKKALKDIDDLPKDYEAPDFFLKEAEQMMLDWSEFDKKS"
     gene            2713..>3161
                     /locus_tag="PXD81_002287"
     CDS             2713..>3161
                     /locus_tag="PXD81_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HDL5538268.1"
                     /translation="MKTFKLLPIMVAFSGMALAETATVAQVASEPVVQDNILPQLSFA
                     AQQARAAELVKQADYALEQEQIRQEQARKFAEAEARIKQEIGNNALLLSQATTKVYLD
                     SEFATMWNDKAAEKAFLKEYALFAVSGVSSKSAKALQQILNQPEGLG"
BASE COUNT         1095 a          497 c          703 g          866 t
ORIGIN      
        1 ttgagcaaaa agtagaacaa actcagaata aaaccaatcc aagtaccaag gaagtgattg
       61 cttatttagc ggagaaattt ccgctttgtt tcacgcttga aggtgaagta aaaccattga
      121 aagttgggtt gtttcaggaa ttagctgaag cattaaaaga tgatgagaaa atcagtaaaa
      181 cgttattgcg ccaagtattg cggaattaca ctaattcttg gcgttacttg gctgccagcc
      241 aagctaatgt tgctcgtgtc ggcttacagg gtgaagaagt tggcgtggta acagaagacg
      301 aagctactca cgccgcagaa gctttggctg ctgcgaaagc ggtggttgca gaacgtaaag
      361 cacaagagcg taaagcacaa cgtaaagagt acttcaaaca gaaagcacgt gaagaaaatg
      421 cgaaaaagcg tgcagaattt aaggcgaaaa aagcaataga taatttacct aaagcttcag
      481 aagaaaaatt agctgcatta gcagataaat tcggtcgtaa atagttttca agtcgctttg
      541 tgcttacaag cggtcggatt ttagtaaaat tttgcaaaaa aatgaccgct tgattatttg
      601 gaaaagaaga tgaaattaac aagattaaat aaaatttttg ccctagccct aggtttaatg
      661 ataggaaacg cttttgccgt tcagccggac attaaagaaa gtgcgattgt gatgccaaaa
      721 cctaatgagc agcatagctt atcaaccaag cgtgtaacag cccgattaac tcaatcgcat
      781 tatcataaat ttcaactgaa tgatgagttt gcagggaaga tttttaaccg ctatgtcgat
      841 tggcttgatc cttctcataa tacttttctt caagccgata ttgatgaaat gcgtagtaaa
      901 tatgctacaa cattagatga tgatttatat gctggtaaat tagatgcagc atttgcaatg
      961 tatgagttaa tgtctaaacg tcgttatgag cgttatagct atgccttatc tttactagat
     1021 aaagagcctg acttaaaagg gaatgatgaa attgagattg accgtgagaa ggccgcattt
     1081 ccaaaaagtg tagaagaggc aaatacactt tgggagcagc gagttaaaaa cgatattatc
     1141 aatttatatc ttaaagataa aaaatggcct gaaattaaga aaacactcac taaacgttat
     1201 aactttgcga ttaagcgttt aactcaagtt aaggctgatg atattttaca aacttactta
     1261 aattcattct ctcgtgagat cgatccccac actagctatt tgtctcctcg tgcggcaaaa
     1321 gcgttccaag agagcatgaa tctgtcgcta gaaggaattg gtgcaacgct aacccaagaa
     1381 gatgatgtaa ccagcattag agcattagtg ccaggagccc ctgccgctaa aagtaagaag
     1441 ctggtagtcg gcgataaaat tgtcggtgta ggtcaaggtg agagaggtga gattgaagat
     1501 gttatcggtt ggcgtttgga taatgtagtc gataaaatca aaggtaaaaa aggcactaaa
     1561 gttcgtttag aaattgagcc tgaaaaaggt ggaaaaacta aaattattac ccttgtgcgt
     1621 gataaagttc gtattgagga tagtgctgct aaattaacgg tagaaaaagt agatggaaaa
     1681 aatgtagggg taattaagat ccctactttt tatatcggtt tgacttcaga tgtacgtaaa
     1741 ttacttactc aaatagagaa aaaaaatgtt gaaggattga tcattgattt acgtgaaaac
     1801 ggtggtggtt cgttaactga agtgattgaa ttaactggtt tatttattaa agatgggcct
     1861 gtagtgcaag tacaagacgc attcaatcgt atcaaagtac acgaagatcc agaatcaaaa
     1921 tcagtttatg atggaaaagt gatggtgatg attaatcgcc atagtgcttc agcctctgaa
     1981 atcttcgctg cggcaatgca agattacaac cgtgcaatta ttgtagggca acaaaccttt
     2041 ggtaagggaa ctgttcaaca gagccgttca ttgaattttg tatatgattt ggatcaagag
     2101 cctcttggtt ttatccaata tacgattcaa aaattctacc gtatcaatgg tggaagtacc
     2161 cagctaaaag gggttgaggc ggatattaaa ttcccggaaa tcattaatgc tgcaaaaaca
     2221 ggtgaaagtt ttgaagataa tgcgcttcct tgggataaaa ttccagcagc taattataaa
     2281 gaggctggga atgctcgtaa cgcggttgct gcattaacag cgaaacatca agagcgtatt
     2341 gctaaaaacc cagagtttat agtattaaac gagaatattg cgattcgaaa agaacgtgat
     2401 gagcgtaagt ttttatcgtt aaatttagaa gaacgcctga aagaaaataa agcagatgaa
     2461 agcaagcgct taaaagatat taatgctcgt tttgcccgtg aaggcaaaaa agcattaaaa
     2521 gatattgatg atttaccaaa agactatgaa gcaccggact ttttcttaaa agaagctgaa
     2581 cagatgatgc ttgattggtc agaatttgat aaaaaatcat aaatgtgttt ataatgcgca
     2641 cataacgtac cgcttagctc cgctaagcgg tttatttttt tataaatttg caaatattaa
     2701 gaggaagagt gggtgaaaac ctttaaatta ttacctatta tggtggcgtt ttctggcatg
     2761 gctttggctg aaacagccac agttgctcag gtggcttctg agcctgttgt acaagataat
     2821 attttaccac aattaagttt tgcagctcag caggctcgtg ctgcagaatt ggtaaaacag
     2881 gccgattatg cgttagaaca ggaacaaatt cgtcaagagc aagcaagaaa atttgcagaa
     2941 gcggaagcaa gaattaagca agaaataggt aataatgcct tattgctgag ccaagcaaca
     3001 actaaggttt atttagacag tgaatttgcg acaatgtgga atgataaagc cgcagaaaaa
     3061 gcatttttga aagaatatgc attatttgca gtaagtgggg tatcctctaa atcagctaag
     3121 gcattgcagc aaatcttaaa tcaaccggaa ggtttaggta g
//
LOCUS       DANLYI010000222         3155 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.224, whole genome shotgun sequence.
ACCESSION   DANLYI010000222 DANLYI010000000
VERSION     DANLYI010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3155
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.224"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            complement(102..1079)
                     /gene="rluD"
                     /locus_tag="PXD81_002288"
     CDS             complement(102..1079)
                     /gene="rluD"
                     /locus_tag="PXD81_002288"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HDL5538269.1"
                     /translation="MTQQKILTAEITADLLGARLDQALAQLFPDYSRSRIKVWIESDL
                     VKVNGNIVNKAREKVFGGETVEITAEIEEEIRFEPQDIPLNIVYEDDDIIVINKPKDL
                     VVHPGAGNPDGTVLNALLHYYPPIAEVPRAGIVHRLDKDTTGLMVVAKNIPAQTHLVT
                     ALQKRRITREYEAIASGIMTQGGKVDEPMARHPTKRTAMAVHPMGKPAVTHYRIMERF
                     RNYTRLRLRLETGRTHQIRVHMAHIAHPLLGDQLYGGRPRPPKGASDSFLSVLRGFQR
                     QALHATMLRLEHPITGEMMEWHAPLPDDFIELIQALKADYELYKDQLDY"
     gene            1220..1999
                     /locus_tag="PXD81_002289"
     CDS             1220..1999
                     /locus_tag="PXD81_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HDL5538270.1"
                     /translation="MRKFKSLATLVLAGLFVAGCSNSNKELEQSNVQDLYGKGQTYLQ
                     DGDYNSAIRYLEAVGTKGGQYSGLGEQTQLSLIYAQYKVGEYYKALDAAERFARSYPN
                     AANMDYVFYLAGLSNARLSDNFIQDFFGVNRASRAVDNVRNAYGNFQTIVQRYPQSQY
                     AQDAQNWMNYLFNRLAEHELAVVKFYDERNAYVAVVNRIEEMLRFYPNTLATKQALPY
                     LKEAYKQMNIPDAEQKTELLIQEYESKELPNPEKLKYGEQF"
     gene            2072..3103
                     /gene="tsaD"
                     /locus_tag="PXD81_002290"
     CDS             2072..3103
                     /gene="tsaD"
                     /locus_tag="PXD81_002290"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005598001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HDL5538271.1"
                     /translation="MRILGIETSCDETGVAIYDEDKGLVANQLYSQIDMHADYGGVVP
                     ELASRDHIRKTLPLIQEALKEANLQPSDIDGIAYTAGPGLVGALLVGSTIARSLAYAW
                     NVPALGVHHMEGHLLAPMLEENAPEFPFVALLISGGHTQLVKVDGVGQYELLGESIDD
                     AAGEAFDKTGKLLGLDYPAGVAMSKLAESGTPNRFKFPRPMTDRPGLDFSFSGLKTFA
                     ANTIKANLNENGELDEQTKCDIAHAFQQAVVDTILIKCKRALEQTGYKRLVMAGGVSA
                     NKQLRADLAEMMKKLKGEVFYPRPQFCTDNGAMIAYTGFLRLKNDEQTDLSISVKPRW
                     AMTELPPIN"
BASE COUNT          938 a          676 c          627 g          914 t
ORIGIN      
        1 tagttaatac taattttttc atagaaagct ccttttttgt tgtttactta tgaaaacgat
       61 ttcaatatag caactttaca ttatttaagc aacacctaaa cttaataatc taattgatct
      121 ttataaagct cataatccgc ttttagtgcc tgaatcaatt caataaaatc atcaggtaac
      181 ggggcgtgcc actccatcat ttcaccagta atcggatgct ctaatctcaa cattgtggcg
      241 tgtaaggctt ggcgttggaa gcctcgtaac accgataaaa acgagtcact cgcaccttta
      301 ggtgggcgag gtctaccgcc atagagctga tcacctaata atgggtgagc aatatgcgcc
      361 atatgtaccc gaatttggtg agtacgccct gtttctaatc ttaaacgtaa acgtgtgtaa
      421 ttacgaaaac gctccataat acgataatgc gtaaccgccg gttttcccat tgggtgaaca
      481 gccattgccg tacgtttagt tgggtggcga gccattggct catcaacttt tccgccctgt
      541 gtcataatgc cgctggcaat cgcttcatat tcacgggtaa tacgacgttt ttgtaatgcg
      601 gttactaagt gagtctgtgc cggaatgttt ttagccacta ccattaagcc tgtcgtgtct
      661 ttatccaatc ggtgaacaat ccctgctcga ggcacttctg caatcggtgg ataataatgt
      721 agcaaagcat tcagcactgt accgtccgga ttaccagccc ctgggtgaac cactaaatcc
      781 ttcggtttat taatgacgat aatatcgtca tcttcataca caatatttag aggaatatct
      841 tgaggctcaa aacgaatttc ctcttctatt tcagcggtaa tttcaacagt ttcaccacca
      901 aatacttttt cccttgcttt attcacgata ttgccattaa ctttcactag atcactttca
      961 atccacactt taatgcgtga acgagaatag tccggaaaaa gttgtgccag agcctgatct
     1021 aggcgagcac ctagtaaatc tgcagtaatt tctgctgtta aaatcttttg ttgagtcatt
     1081 aaaccataaa tcctaattag tcatttgctt acttgcgtga tgttatatag aatagcgaga
     1141 tgaacttcat aagttattct tagtcagagg acattgtaca ttaacttttc gtcctagaca
     1201 ctttttatag gtaaaacata tgcgtaaatt taaatctctt gccacattag tattagccgg
     1261 actgtttgtt gcaggttgtt caaattcaaa taaagaatta gaacaatcca acgttcaaga
     1321 tctttatggc aaaggtcaga cctatttaca agatggagat tataattctg ccattcgcta
     1381 cttagaagcg gttggaacca aaggaggcca atattcagga cttggtgagc aaacccaact
     1441 aagtttaatt tatgctcaat ataaagttgg cgaatactat aaagcacttg atgccgcaga
     1501 acgttttgct cgctcttacc cgaatgcagc taatatggat tacgtattct atctagcagg
     1561 attatcaaat gcacgtctaa gtgataattt tattcaagac ttctttggtg taaatcgcgc
     1621 ctctcgagcc gtagataatg ttcgtaatgc ttacggtaac ttccaaacta ttgtacagcg
     1681 ctaccctcaa agccaatatg cacaagatgc tcaaaattgg atgaattact tatttaaccg
     1741 tttagcggaa cacgaattag ccgttgttaa attttatgat gaacgtaatg cctatgtggc
     1801 tgtagtaaac cgtatcgaag aaatgttgcg tttttacccg aatacactgg caaccaaaca
     1861 agcattgcca tacttaaaag aagcatataa gcaaatgaat atcccagatg cagagcaaaa
     1921 aacggaactc ttgatccaag aatatgaaag caaagagcta ccgaatcctg aaaaactgaa
     1981 gtatggcgaa caattctagt cgtacagaga ataatgtgag gggcgttctt cgcccctttt
     2041 ggttttctaa cttattttga cttctccaac tatgcgaatt ttaggtattg aaacctcttg
     2101 tgatgaaacc ggtgttgcca tttatgatga agacaaaggc ttagtggcaa accagcttta
     2161 tagccaaatt gatatgcacg ccgattacgg tggcgtagtc cctgaactgg cttctcgaga
     2221 ccatatccgt aaaacgttgc cactaattca agaagcctta aaagaggcca atctgcaacc
     2281 ctcggatatt gacggcattg cctatactgc cggcccaggc ttggtcgggg ctttattggt
     2341 cggctcaacc attgcccgtt cgctggctta tgcttggaat gttccggcat tgggcgttca
     2401 ccatatggaa gggcatttac ttgccccaat gttggaagaa aatgcccctg aatttccgtt
     2461 tgtggcatta ttgatttcag gtggacacac ccaactggta aaagttgacg gcgttgggca
     2521 atacgaacta ctcggggaat caattgatga tgctgccggt gaagcctttg acaaaacagg
     2581 caaactactc ggtttggatt accctgccgg tgtagcgatg tcaaaattag ccgaatccgg
     2641 cacgccaaat cgttttaaat tccctcgtcc aatgaccgac agaccgggac tggatttcag
     2701 tttctccggt ttaaaaacct ttgctgcgaa tacgattaaa gccaatctta atgaaaatgg
     2761 tgaactcgat gagcaaacca aatgcgatat tgcccacgca ttccaacaag ccgtggttga
     2821 tactatttta attaaatgca agcgagcgtt agagcaaacc ggctataaac gcttagtaat
     2881 ggcaggcggc gtaagtgcca ataaacaatt acgagcagac cttgcggaaa tgatgaaaaa
     2941 attaaaaggc gaagtattct accctcgccc acaattttgc actgacaacg gcgcaatgat
     3001 tgcctacact ggctttcttc gcttaaaaaa cgatgaacaa accgacttaa gcattagcgt
     3061 aaaaccccgc tgggctatga ccgaattacc accgattaat taacctttca agcggtgaaa
     3121 tttcttgtta attttgcaaa aatttaatca aaaat
//
LOCUS       DANLYI010000223         3148 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.225, whole genome shotgun sequence.
ACCESSION   DANLYI010000223 DANLYI010000000
VERSION     DANLYI010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3148
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.225"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            complement(3..1187)
                     /locus_tag="PXD81_002291"
     CDS             complement(3..1187)
                     /locus_tag="PXD81_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HDL5538272.1"
                     /translation="MTSSNTIKQLDKDYIAQTYGRFDLALSHGKGCEVWDFEGNKYLD
                     FTSGIGVNSLGWADEDWLQAVTTQAGKLAHTSNLFFTEPASQLAQKLVAASGLKRAFF
                     CNSGAEANEGAIKTARKYSFDKYGSGRSTVLTLVNSFHGRTISTLASTGQEVFHKHFF
                     PFTEGFEYLPVNGIEALKARLETENVCGILLEVVQGEGGVCPLEADYLHAVQSLCQEK
                     DILFMIDEVQTGIGRTGTLFAYQQFDLTPGLVTLAKGLGGGLPIGAFLLGEKVEMTLG
                     KSDHGSTFGGNPVSCAAANVVIDKINAEFLAQVQQKGQLLQQRLASLPKVKSVSGLGL
                     MLGVEFEEGILAADIVAKCINKGVLFLTAKSKLRLLPPLMISEQQIEQGIRVLEDVLK
                     DS"
     gene            complement(1200..2054)
                     /gene="argB"
                     /locus_tag="PXD81_002292"
     CDS             complement(1200..2054)
                     /gene="argB"
                     /locus_tag="PXD81_002292"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005611371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HDL5538273.1"
                     /translation="MQQQEINQFANLLEKSTACLVKWQGKIIVVKYGGNAMINEELKK
                     SVMADLLLLHRLGVKVVLVHGGGLEISQGVKLLGKEPQFINGLRVTDSDTINVVLQML
                     AGKVNKSLVALLKGKGIGLCGIDGAMLQCKKLEAEVDYGFVGDIVKVDPTVIEIALNA
                     GLIPVVSTVGVDENGQAYNINADTAASEIAIALQAEKLVSMTDIAGLLRDRFDESTLI
                     PEVEVSEVQSLIEQGIIAGGMIPKIACCTDFIRAGGSEANIIDGRIDHAILVEMFGGK
                     NGTRFYKK"
     gene            complement(2146..3087)
                     /gene="argC"
                     /locus_tag="PXD81_002293"
     CDS             complement(2146..3087)
                     /gene="argC"
                     /locus_tag="PXD81_002293"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HDL5538274.1"
                     /translation="MSKYKIFVDGAVGTTGLRIHERLSQADDIELLTLPEEQRKDLNA
                     RVAKVAEADLTFLCLPDEASKELVANAPENTRICDTSTAHRVNPNWVYGFPELSGQRE
                     KIQNAKRVAVPGCHATGYIALVRPLVEKGILPADYPLSCHSLTGYSGGGKAMIADYEN
                     ENSPNAFNAPRVYGLTLQHKHLPEMQAVTQMQNPPIFTPVVADYYSGMLVTVPLQIFA
                     LQAVKSGEEIANLLADYYQTSRLIHVHPFQSLPEDGMLAANAYAGKDSLEIFIYGNST
                     QLLLAARFDNLGKGASGAAVQCMNIMLGRDEVEGLEL"
BASE COUNT          820 a          792 c          632 g          904 t
ORIGIN      
        1 actcaagaat ctttcaagac atcctctaac actctgattc cttgttctat ctgttgttca
       61 gagatcatca gcggtgggag tagccttaac ttgcttttag cggttaaaaa cagcacacct
      121 ttgttgatac attttgcaac aatatcagcg gctaaaatgc cttcctcaaa ttctacgccg
      181 agcattaaac ctaagcctga aacggattta acttttggta agctcgctaa gcgttgctgt
      241 aacaactgcc ctttttgttg cacttgggcg agaaattcgg cgtttatttt atcgattact
      301 acatttgcag cagcacagga aacaggattg ccaccaaagg ttgagccgtg gtcgcttttg
      361 cctaatgtca tttccacttt ttcgcctaac aggaaagcac caatcggcaa gccgccgccc
      421 aagcctttag cgagcgtgac taaaccagga gttaaatcaa attgttgata agcaaaaagt
      481 gtgcccgttc gcccaatgcc ggtttgcacc tcatcaatca taaataaaat atctttttct
      541 tggcacaggc tttgcacggc gtgcagataa tccgcctcta atgggcaaac accgccctcg
      601 ccctgcacca cctccagcaa aatcccgcaa acgttctcag tttccaagcg agctttcagt
      661 gcctcaatgc catttacagg taaatattca aagccctctg taaaggggaa aaaatgcttg
      721 tggaatacct cttgcccagt ggatgcgagg gttgaaatgg ttcgtccgtg aaaagagttc
      781 acgagggtta aaaccgttga acgaccggaa ccgtatttgt cgaaactata tttgcgggcg
      841 gttttaattg ctccttcatt tgcctctgcc cctgaattgc aaaaaaatgc ccgttttaga
      901 ccgcttgcag ccactaattt ttgggctaat tggcttgccg gttcggtaaa aaacaggttt
      961 gaggtgtgag ctaatttgcc ggcttgtgtg gtaacagctt gtagccaatc ctcatccgcc
     1021 cagcctaaac tatttacccc aatgccactg gtaaaatcaa gatacttatt gccctcaaaa
     1081 tcccacactt cacagccttt tccgtgcgac agtgcaaggt caaatctgcc ataggtttgg
     1141 gcgatataat ccttatctaa ttgcttaatc gtattactgc ttgtcatttc actttcctgt
     1201 tattttttat aaaaccgtgt gccgtttttg ccaccgaaca tttctaccaa aatggcgtgg
     1261 tcgattcgcc cgtcaataat atttgcctca gagccaccag ctctgatgaa atcggtacag
     1321 cacgcaattt tggggatcat cccaccggca ataatgcctt gctcaatcag ggactgcact
     1381 tcgcttactt ccacttccgg aattaaggtg ctttcatcaa agcgatctcg caataaacct
     1441 gcaatatcgg tcatcgagac caatttttcc gcttgtaacg caatcgcaat ctcgctggca
     1501 gcggtgtctg cattaatgtt ataagcctgc ccgttttcat caacgcccac cgtggaaaca
     1561 accggaatca agcccgcatt aagagcaatt tcaatcacag ttggatcgac tttcacaata
     1621 tcgcccacaa agccgtaatc cacctctgcc tctaattttt tgcattgcaa catcgcccca
     1681 tcaataccac acagcccaat gcctttccct tttaataagg cgacaaggct tttattaact
     1741 ttgcctgcca acatttgcaa aaccacgtta atcgtatcgc tatcggtaac acgcaacccg
     1801 ttgataaatt gcggttcttt acccaataat ttcacgcctt gtgaaatctc cagcccgcca
     1861 ccgtgtacca gtaccacttt tacgcctaaa cgatgaagta acagcagatc tgccatcact
     1921 gattttttca gttcctcatt aatcattgcg ttaccgccat atttcaccac aatgatttta
     1981 ccctgccatt tcaccaaaca agcggtggat ttttctaata aatttgcaaa ttgattgatt
     2041 tcttgttgtt gcatatttct ttctcttgta gggacgggtc ctgtgcccgt ccgaatatgg
     2101 gcgatgatta tcggacaggc acaggacctg tccctacatt tatatttaaa gctctaaccc
     2161 ctcaacctca tctcttccca acataatatt catacactgc actgcggcac ctgatgcccc
     2221 tttgcctaaa ttgtcaaaac gggcagcaag taaaagttgg gtgctgttgc cgtaaataaa
     2281 aatctctaag ctgtctttac ctgcataagc attcgcggca agcattccgt cttcgggtag
     2341 agattggaaa gggtgaacgt gaattagtcg gctggtttgg tagtaatcgg cgagcaaatt
     2401 tgcaatttct tcgccggatt tgaccgcttg taaggcgaag atctgcaatg gcacagtgac
     2461 taacattccg ctgtaataat cagcaaccac aggggtgaaa atcggtgggt tttgcatttg
     2521 ggtaactgcc tgcatttcag gcaaatgttt gtgttggaga gttaaaccgt aaacacgagg
     2581 ggcattgaaa gcatttgggc tgttttcatt ttcatagtca gcaatcatcg ctttgccacc
     2641 gcctgaatag ccggttaagg aatgacaact taaagggtaa tcggcgggca gaatcccttt
     2701 ttccaccagt ggacgaacca acgcaatata gcccgtggca tggcaacccg gcaccgcaac
     2761 acgcttggca ttttggattt tttcacgttg tccgctcaat tccggaaaac cataaaccca
     2821 attaggattg acccgatgag cggttgaagt atcgcaaatt cgggtatttt ccggtgcatt
     2881 tgctactaat tcttttgagg cttcatccgg caaacagagg aaagttaaat ccgcctctgc
     2941 gacttttgcc acacgggcgt tcaaatcttt ccgctgctct tcgggcaagg ttaataattc
     3001 aatatcgtcc gcttggctta aacgttcgtg aatgcgtagc ccggttgttc ctactgcccc
     3061 atcaacaaaa atcttgtatt tgctcattga aaatccttct ttgacttttt ctgctcggta
     3121 gtctaaggct atttaaggct aaactcaa
//
LOCUS       DANLYI010000224         3093 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.226, whole genome shotgun sequence.
ACCESSION   DANLYI010000224 DANLYI010000000
VERSION     DANLYI010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3093)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3093)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3093
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.226"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            complement(<1..232)
                     /locus_tag="PXD81_002294"
     CDS             complement(<1..232)
                     /locus_tag="PXD81_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd biosynthesis protein"
                     /protein_id="HDL5538275.1"
                     /translation="MIHKLYNLTAKGLLKALSFILAAVLFATIWVNSTAFALSFGGKT
                     PYLAMLVFYGMAILWVHGIGFEIRAAIWKVIFL"
     gene            complement(232..330)
                     /locus_tag="PXD81_002295"
     CDS             complement(232..330)
                     /locus_tag="PXD81_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CydX/CbdX family cytochrome bd oxidase small
                     subunit"
                     /protein_id="HDL5538276.1"
                     /translation="MFYVTWVLGVLLAIFFATVITISIEKTGKFDE"
     gene            complement(345..1478)
                     /gene="cydB"
                     /locus_tag="PXD81_002296"
     CDS             complement(345..1478)
                     /gene="cydB"
                     /locus_tag="PXD81_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HDL5538277.1"
                     /translation="MIDYEILRFIWWILIAVLLIGFAITDGFDMGVLNLLPIIGKSNV
                     ERRIMINTIAPHWDGNQVWLLTAGGAIFAAWPTVYATSFSGFYIAMVLVLAALFFRPV
                     GFEYRAKIDNPKWRSAWDWGLFIGGFVPSLVFGVAFGNLLQGVPFEFNNINQSQYTGS
                     FFGLLNPFALVCGIISLMMLTTQGATWLQMKTTDELHNNSRKVAQVTASIVLVAFILA
                     GVWLYFKDGFVITSELDHNAQSLLTNKTVAVQAGAWFRNYAEMPVLWVVPALVVVGAL
                     LTIIASKANRSGLAFFSSSLMIVGVILTAAVSMFPFIMPSITHPEMSLTVWDATASHT
                     TLTVMLFVAGIFVPLVLAYTIWSYIKMYGRLDASYLEENKAAY"
     gene            complement(1491..3041)
                     /locus_tag="PXD81_002297"
     CDS             complement(1491..3041)
                     /locus_tag="PXD81_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HDL5538278.1"
                     /translation="MLDVVELSRLQFALTALYHFLFVPLTLGLSFVLVVMETLYVTTG
                     KEVYKDMTKFWGKLFGINFALGVTTGITMEFQFGMNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWERLSKAKHLLATYCVAFGSNFSALWILVANGWMQNPIASEFN
                     FETMRMELSSFSELVLNPVTQSKFLHTVTAGYVCGAIFVLGISSYYILKGRDLGFAKR
                     SFSVGASFGLIAIISVLIMGDESGYEIGKAQPTKLAAMEGEFETQPAPAAWNAFVIPN
                     TAEMKNEFSIHIPYAAGIIATRSLDKEIKGLKDIRVINEQRVRTGMVAYGLLEKLRSG
                     NYTEADKEAFKAVQKDLGFGLLLKQYTPNVVDATEEQIQKAADNTIPNVGPTFWAFRV
                     MMLSGFLMLILITTAFIQNMRGKVESSRFLLRGLLFGLPLPWIAIESGWFLAEYGRQP
                     WAIYEMLPVGVSNSALTTGDLWFTIGLLCGLYTLFLVVEMYLMFKYGRLGPSALKTGR
                     YYFEQSTK"
BASE COUNT         1099 a          640 c          499 g          855 t
ORIGIN      
        1 gtaaaaaaat cactttccaa atagcagctc ggatttcaaa tccaattcca tgtacccata
       61 aaatagccat accataaaat accaacattg ctaaatacgg cgtttttcca ccaaaagaaa
      121 gagcgaaagc ggtcgaattt acccaaatag ttgcaaataa tacagccgct aaaataaaag
      181 aaagggcttt taacaagccc tttgctgtta agttatagag tttatgaatc attactcgtc
      241 aaacttacct gttttttcaa tgctgatcgt gatgactgtt gcaaagaaga ttgcaagcaa
      301 tacacctaag acccaagtta cataaaacat attgtgtctc cttattagta agctgcttta
      361 ttttcttcta aataactagc atctaaacga ccatacattt tgatatatga ccaaatagta
      421 tatgctaata ctaacggtac aaagatacca gcaacaaata acattacagt taatgttgtg
      481 tggctagcag tagcgtccca aaccgttaag ctcatttctg gatgagtaat tgacggcata
      541 atgaatggga acattgatac tgcagcagtt aaaatcaccc caacaatcat caatgatgat
      601 gagaagaatg ctaaacctga acgatttgct tttgaggcaa taatagtcag taatgcacct
      661 actacaacta atgcaggaac aacccataat acaggcattt cagcataatt tctaaaccat
      721 gcaccagctt gaaccgctac agttttattc gtcaatagag attgtgcatt atgatctaac
      781 tcgcttgtga tcacaaaacc atctttaaaa tataaccata cacctgcaag aataaatgcc
      841 actaatacaa tagaagctgt cacttgtgcc acttttcttg agttgttatg tagctcatca
      901 gtagttttca tttgtaacca agttgcaccc tgtgtggtta acatcattaa gctgataata
      961 ccgcacacta atgcaaatgg atttaataaa ccaaagaatg aacccgtata ttgtgattgg
     1021 ttaatattat taaattcaaa tggtacacct tgtaataagt taccgaatgc aacaccgaat
     1081 actaatgatg gaacgaaacc accaataaat aatccccaat cccaagcact acgccatttc
     1141 ggattatcaa tttttgcacg atactcaaaa cctacagggc ggaaaaataa tgctgcaagc
     1201 acaagcacca ttgcaatata gaaacccgag aacgaagttg catagactgt tggccatgca
     1261 gcgaagatcg caccaccagc ggtcaataac catacttggt taccatccca gtgaggggca
     1321 attgtgttga tcataatacg acgttcaaca ttacttttac caatgatagg taaaaggttt
     1381 aatacgccca tatcaaaacc atcagtaatt gcaaagccaa ttaacaatac cgcaattaag
     1441 atccaccaaa taaagcggag aatttcataa tcgatcattt ctaactccta ttatttagtt
     1501 gattgttcaa aataataacg gcctgttttt aatgcactag ggccaagacg accatatttg
     1561 aacattaaat acatttctac aactaagaat agtgtatata aaccacacaa taagccgatt
     1621 gtaaaccata aatcacctgt tgttaatgca gagtttgaca caccgacagg taacatttca
     1681 tagatagccc aaggctgacg gccatattcg gcaaggaacc aaccagattc aatcgcaatc
     1741 caaggtaacg gaagaccgaa taacaagcct cttaataaga aacggctact ttctacttta
     1801 ccacgcatat tttgaataaa tgctgtcgta atcaagataa gcatcaagaa gccagataac
     1861 atcattacac ggaatgccca gaatgtaggg cctacatttg gaattgtatt atctgctgct
     1921 ttttgaattt gttcttcagt cgcatcaacc acattaggtg tatattgttt taacaataac
     1981 ccaaagccta aatctttttg tacagcttta aatgcttctt tatccgcctc tgtataattg
     2041 ccagaacgta atttttctaa tagtccgtag gctaccatac cggtacgaac acgttgttca
     2101 ttaattacac gaatatcttt taggccttta atttctttat ctaaagaacg ggttgcaata
     2161 ataccagctg cataaggaat atgaattgag aattcatttt tcatttccgc agtatttgga
     2221 atcacaaaag cattccaagc agctggagca ggctgagttt cgaattcacc ttccattgca
     2281 gctaatttag tcggctgagc tttaccgatt tcataaccag attcatcacc cataatcagt
     2341 acagagatga tcgcaattaa accaaaactt gcacctactg agaatgaacg ttttgcaaag
     2401 cctaagtcac gaccttttaa gatatagtat gaactaatac ctaatacaaa aatagcacca
     2461 caaacatagc ctgcggttac agtatgtaag aatttagatt gtgtaactgg gtttaataca
     2521 agttcagaga aacttgaaag ctccattcgc atggtttcaa agttgaattc agaagcaatc
     2581 gggttttgca tccaaccatt agcaactaaa atccatagtg cagagaagtt agaaccaaac
     2641 gcaacacagt aagttgctaa taaatgtttc gctttcgata aacgttccca gccaaagaag
     2701 aacaagccca caaaggtaga ttctaagaag aacgccatta aaccttcaat cgctaatgga
     2761 gcgccaaaaa tatcacctac ataatgagaa taatatgacc agttcatacc aaactggaat
     2821 tccattgtaa taccagttgt tacccctagg gcaaagttaa taccaaataa tttaccccag
     2881 aatttagtca tatctttata tacttcttta cccgtagtta catacagggt ttccatcacc
     2941 acaaggacga aagaaagacc taatgtaagc ggtacgaata agaagtgata gagtgcagtt
     3001 agagcaaact gcaaacgtga gagttcaact acatctaaca tctcaaacct cttatattaa
     3061 tatactaatt tgacaattag attactgtaa aag
//
LOCUS       DANLYI010000225         3036 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.227, whole genome shotgun sequence.
ACCESSION   DANLYI010000225 DANLYI010000000
VERSION     DANLYI010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3036
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.227"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            complement(<1..340)
                     /locus_tag="PXD81_002298"
     CDS             complement(<1..340)
                     /locus_tag="PXD81_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014668079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CMP-N-acetylneuraminate-beta-galactosamide-
                     alpha-2, 3-sialyltransferase"
                     /protein_id="HDL5538279.1"
                     /translation="MNLIICCTPLQVLIAEKIIEMHPNERFYGVMLSTVKNAKFDFYQ
                     ARLAQKCEQFFAMQQHSERIQLLKENIYLKRTFGGKKFNKVFLANINELEIQFLLSAI
                     SFQELNTFDDG"
     gene            complement(337..1542)
                     /locus_tag="PXD81_002299"
     CDS             complement(337..1542)
                     /locus_tag="PXD81_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005694190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="HDL5538280.1"
                     /translation="MKAVKDSVIYLIGELASKSIPFLLLPYFSRKLGVAGYGELSYYQ
                     TFAALFVIFIGLSQDGAVARYFYFYGKRSLNLVLSTGYLYTLCATLLILLGCWYFQSE
                     IIAYVAIGAMFQSFLNVQLSVRQCQKQALAYTTIQLMSAIFSAVITVAMMEYFKTDLV
                     EKRILAILMSNGLVFLIAYFLYAKRAKTKKFSLARHKTALLYLFGFGIPLILHHSSIF
                     LKGQLDRVFIYHKFSEEDLGLYAMGAQIAMILAIVVQALNKATLPYFYEALKQKKINV
                     SHIHKWALYALFIVPLPAIVMWFIPEELVVWILGSQFVGTKYYIILFLIATMLVVPYL
                     ILVNYLFFYGKNKLISLCSILSTIFYLVALFALTFSKIEYIPFASIIGAISIIPILYI
                     MTAKVGKTV"
     gene            complement(1552..2736)
                     /gene="neuC"
                     /locus_tag="PXD81_002300"
     CDS             complement(1552..2736)
                     /gene="neuC"
                     /locus_tag="PXD81_002300"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (hydrolyzing)"
                     /protein_id="HDL5538281.1"
                     /translation="MKNIAYITGSRAEYGIVKRLLQKLAQDPTVNFSLVVTAMHLDPQ
                     YGNTVTVIEQDGFEIAARIPLTLNSENNQTIIHSMAECLDRFGEHFQQHRYDAVIVLG
                     DRYEMLAVATAAAMHNIPLIHLHGGEQTLGNYDEFIRHCITKMSKLHLTSTEEYRQRV
                     IQMGELPENVVNTGSLGAENSLDLVLPTQAELVERLGIPASPYFLVVFHPETLTDVSV
                     LEQIEALLNAFEQFKDKYQFVFIGSNSDTNSEQIYARFKAYTDENQFKFFTSVKPEEY
                     LALIKYSNGLIGNSSSGLIEAPSCGVGTLNIGNRQQGRVRDESVIDVESNEQAIIDGI
                     EKLISADFQHRLPSMKNPYFQDNAMEKAYNAIKIFLADSHHTQPKGFYDVAFSQAVKK
                     SE"
     gene            complement(2737..>3036)
                     /locus_tag="PXD81_002301"
     CDS             complement(2737..>3036)
                     /locus_tag="PXD81_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase"
                     /protein_id="HDL5538282.1"
                     /translation="EKILADINHLIAQLTAIRGDINLYFLEITPTAFRVDRNNNDIRL
                     LNQYLKDNLAVKWISLDQAFSDKYGKLDLSYSDDGLHLNAKGYEKLTAILEKELN"
BASE COUNT          999 a          619 c          510 g          908 t
ORIGIN      
        1 tgccatcatc aaaggtattg agttcttgaa atgaaatggc acttaacaga aattgaattt
       61 ctaactcatt gatatttgct aaaaagactt tattaaactt cttaccgcca aaagtacgtt
      121 ttaaataaat attttctttt aataattgaa tacgctcgct atgttgttgc atcgcaaaaa
      181 attgttcgca cttttgggct aatctagctt ggtaaaaatc aaatttcgcg ttttttaccg
      241 tagaaagcat caccccatag aatcgctcat tagggtgcat ttcaatgatt ttttcagcaa
      301 tcaacacttg caagggcgtg caacaaataa ttaaattcat acggtttttc ctacttttgc
      361 cgtcattata taaagaatcg gaataatgct gattgcacca atgatgctgg caaaaggaat
      421 atattcaatt ttagaaaaag ttaaagcaaa tagtgcaact aaataaaaaa tagtagataa
      481 aatcgagcaa agcgaaatta gcttattttt gccataaaaa aacaaataat tgactaaaat
      541 gaggtaaggc acaaccaaca ttgtcgcaat taaaaagaga ataatgtagt attttgttcc
      601 tacaaattgg ctacccagaa tccataccac taattcttcc ggaataaacc acatcacaat
      661 agcaggtaat ggtacaataa ataaggcata caacgcccat ttgtggatat ggctgacatt
      721 aatttttttc tgctttaagg cttcataaaa ataaggcagt gttgctttat ttaaggcttg
      781 aaccacaatg gctagaatca tcgcaatttg agcccccatt gcatataaac ctaaatcctc
      841 ttcgctaaat ttatggtaaa taaatactct gtctaactgt ccttttaaga aaatactgga
      901 atggtgcaaa atcaacggaa taccaaagcc gaataaatac aataaagcag ttttatggcg
      961 ggcaaggcta aatttcttcg tttttgctct tttagcatac aaaaagtaag cgattagaaa
     1021 gactaagcca ttactcatca aaatcgctaa aatccgcttt tccactaaat cggtcttaaa
     1081 atactccatc attgcaacgg taataactgc cgaaaaaatc gccgacatta attgaatcgt
     1141 tgtataagca agtgcctgtt tttgacactg ccttacacta agctgtacat tcaaaaacga
     1201 ctggaacatc gccccaatcg ctacataagc aataatttca gactggaaat accagcaacc
     1261 caacaggata agcaaagttg cacaaagagt atataaatac cctgtactca agactaaatt
     1321 tagtgaacgc ttaccataaa aatagaaata gcgagcaacc gcaccgtctt ggctaagccc
     1381 gataaaaatt acaaataaag cagcaaaggt ttggtagtag gaaagctccc cataacctgc
     1441 cacgccgagt ttgcgtgaaa agtagggtaa gagcaagaaa ggaattgact ttgacgccaa
     1501 ttccccaatt aaataaatca cactgtcttt aactgctttc atagttgcaa attattctga
     1561 ttttttgacc gcttgcgaga aagcaacatc ataaaatcct ttcggttgag tatggtggct
     1621 gtctgctaag aatattttga ttgcattgta agccttttcc atcgcgttgt cttgaaaata
     1681 tggatttttc atactcggta aacggtgttg gaaatcggct gaaatcaatt tttcgatacc
     1741 atctatgata gcctgttcat tactctctac atcaattacg ctctcatccc gtactcgccc
     1801 ttgctggcga ttaccgatat ttagcgtacc aacaccacaa ctcggtgctt caatcaagcc
     1861 tgatgagcta ttaccaatca acccatttga atatttaatc aacgctaaat actcttccgg
     1921 tttgactgag gtgaaaaact taaattgatt ttcatcggtg taagccttaa aacgtgcata
     1981 aatttgctct gaattggtgt ctgaatttga gccgataaag acgaattggt atttgtcttt
     2041 aaactgctca aaagcattga gtaacgcctc aatttgctcc aacacggaaa catcggttaa
     2101 ggtttccggg tggaagacca ctaaaaaata aggcgatgcc ggaatcccta atctctcgac
     2161 taattctgct tgggtcggca acactaaatc caaactattc tccgccccta gagaaccagt
     2221 attcaccaca ttttcgggca gctcgcccat ttgaatcaca cgctgtctat attcttcagt
     2281 tgaagttaaa tgcaatttcg acattttggt aatacaatgg cgaataaatt catcgtaatt
     2341 acctaatgtt tgctcaccac cgtgcagatg aatcagcgga atattatgca tcgccgcagc
     2401 cgttgccacc gccaacatct cgtaacgatc gcctagcaca atcaccgcat cataacgatg
     2461 ttgctggaaa tgttcgccaa atcgatctaa acactctgcc attgaatgaa taatggtctg
     2521 attattttca ctgtttaagg taagcggaat acgagctgca atctcaaagc catcttgctc
     2581 aatgaccgtc accgtattgc catattgcgg atccaaatgc atcgcagtaa caaccagaga
     2641 gaaattcaca gtcggatctt gtgctaattt ttgtaataaa cgctttacaa tcccgtattc
     2701 ggcacgagat ccggtaatat aggcaatatt tttcatttag ttgagctctt tttccaaaat
     2761 agcggttaat ttttcataac cttttgcatt taaatgtaat ccgtcatcgc tatagcttaa
     2821 atccaatttg ccatatttat cgctaaatgc ctgatctaag ctaatccatt tcaccgctaa
     2881 attatcttta agatattgat ttaacaagcg aatatcgttg ttattacgat cgactctaaa
     2941 tgcagttggg gtaatttcca agaaatagag attaatatct ccacgaattg ctgttaattg
     3001 tgcaattaaa tgattaatat ctgctagaat tttttc
//
LOCUS       DANLYI010000226         2911 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.228, whole genome shotgun sequence.
ACCESSION   DANLYI010000226 DANLYI010000000
VERSION     DANLYI010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2911
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.228"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            237..1355
                     /locus_tag="PXD81_002302"
     CDS             237..1355
                     /locus_tag="PXD81_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HDL5538283.1"
                     /translation="MKKTLVALAVTAFAASASATTVYDAEGSKVDFDGSLRIVLDRTN
                     SKELDNHNVVKTKKDAHTGIRNTGSRFGVRVKHDLAQDFYALGRVEFRFNKNTSDDGF
                     GDLYTKRAYVGLGSKQYGELTFGRQVTIADDLSQSVDYNYGINPKGDYIQTDGNQVIR
                     YNYRGVENLHVSANYNFAQDRKDGKVLANKLQNAYGIGALYTVDNWDLRAAYGHTNYK
                     TATDSTKHRKDGVLGSVGYKFGDLKLSVDTGYSREKGLAVTDDNGVTTFYNGVKSNAF
                     YVSPNFEYQVVPASKVYGNYLYERVKSKSNGSTYAAGKEKTHGFLLGVDYQFHKQVVA
                     FVEGSYTRTKDYDKVTNGYEYSGKSTNKAIGVGMRVYW"
     gene            complement(1508..2872)
                     /gene="glmU"
                     /locus_tag="PXD81_002303"
     CDS             complement(1508..2872)
                     /gene="glmU"
                     /locus_tag="PXD81_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012262868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HDL5538284.1"
                     /translation="MTQLSVVILAAGKGTRMYSDLPKVLHPVAGKPMVKHVIDTAKQL
                     DAQQIHLIYGHGADLLKARLSEEPVNWVFQAEQLGTGHAMQQAAPFFADDENIVMLYG
                     DAPLITKATLERLIAAKPENGIALLTVELENPTGYGRIIRENGSVVAIVEQKDANEAQ
                     LKVKEVNTGVMVASGASFKKWLKNLNNNNAQGEYYITDVIAMANQEGFKVQAVQATEF
                     MEVEGANNRLQLAALERFYQKREAEKLLLAGVTIIDPSRFDLRGTVTHGKDVQIDVNV
                     ILEGEIKLGNNVKIGAGCVLKNCEIGDNVEIKPYSVIEDSIVGAKSAIGPFSRLRPGA
                     ELAEETHIGNFVEIKKATIGKGSKVNHLTYVGDAEIGKECNIGAGVITCNYDGANKFK
                     TIIGDNVFVGSDSQLIAPVTIASGSTIGAGATVTKDIAENELVISRVPQRHIQGWQRP
                     TKKK"
BASE COUNT          840 a          530 c          572 g          969 t
ORIGIN      
        1 aatgagtgga ttttctccaa atgaaataat gtaagtataa ccatttgatt ttaaaagaaa
       61 aaatgccgtc tttgttttgg ggtttgaact attgacattg agttttcttt ttgcaattat
      121 tctggcgaaa acaatgttag gcgttttgct gatacattgt atcttggata attactctta
      181 caccttaatg agggtaatta cttatcatat aacatataaa atctaaggtg aaaattatga
      241 aaaaaacatt agttgcatta gcagtaaccg cgttcgcagc gtcagcatca gcaaccaccg
      301 tttacgatgc agaaggttca aaagtagatt ttgatggttc tttacgtatt gttcttgata
      361 gaacaaattc taaagagcta gacaatcata atgttgttaa aactaaaaaa gatgcacaca
      421 caggaatccg taacactggt tcacgttttg gtgtaagagt aaaacatgat ttagctcaag
      481 atttctatgc attaggtcgt gttgagttcc gttttaacaa aaataccagt gatgatggtt
      541 ttggtgattt atatactaaa cgtgcttatg ttggtttagg tagtaagcaa tatggtgaat
      601 taacttttgg tcgtcaagta acgattgctg atgatttaag tcaatcagta gattacaact
      661 acggtattaa tccaaaaggt gattatattc aaaccgatgg taaccaagtt attcgttata
      721 actaccgtgg tgttgaaaac ttacatgtaa gtgcaaacta taactttgca caagatcgta
      781 aagatggtaa agtattagca aataaacttc aaaatgcata cggcattggt gcattataca
      841 ctgtagataa ctgggattta cgtgcagctt atggtcatac aaactacaaa acagctactg
      901 atagcactaa gcaccgtaaa gatggtgtat taggttctgt tggttataaa tttggtgact
      961 taaaattaag tgttgataca ggttattctc gtgagaaggg tttagctgtt acggatgata
     1021 atggtgtaac tacattctat aatggtgtta aatcaaacgc tttctatgta tctccaaact
     1081 ttgaatatca agtagtgcct gcatctaaag tatatggtaa ctacttatat gagcgtgtga
     1141 aatctaaatc aaatggttct acatatgctg ctggtaaaga aaaaactcat ggtttcttac
     1201 ttggtgttga ttaccaattc cataaacaag tagttgcttt tgttgagggt tcttatactc
     1261 gtactaaaga ctatgataaa gtaactaatg gttatgaata ctcaggcaaa tcaactaaca
     1321 aagctatcgg tgttggtatg cgtgtatact ggtaatcaat tgattttcta aattgataaa
     1381 cccctaacaa tatttattgt taggggtttt gttttaaata tattgttcat tatccttttt
     1441 aaagtgtatg gctttgctgt tattggcaaa gccaattttt aaatataata acttttgacc
     1501 actggcacta tttcttttta gtcgggcgtt gccagccttg aatatggcgt tgtggtacac
     1561 ggctaattac cagttcattt tccgcaatgt ctttagtgac tgttgcaccg gcaccaatgg
     1621 ttgaaccgct ggcaatcgtg accggggcaa tgagttggct gtctgagccg acaaatacgt
     1681 tatcgccaat aatggttttg aatttattcg caccatcata gttacaagtg attacacctg
     1741 ctccgatgtt acattctttg ccgatttccg catcgcctac ataagtgaga tgatttactt
     1801 ttgaaccttt gccgatggta gcttttttga tttccacaaa gttaccgata tgggtttctt
     1861 ccgctaattc tgcccccggg cgtaagcgag agaatggacc gatagcggat tttgccccga
     1921 caatcgaatc ttcaatcaca gaataaggtt taatttcaac attatcgcca atttcgcagt
     1981 ttttcagtac gcagcctgca ccaattttta cattgttacc gagtttgatt tcaccttcta
     2041 gaatgacatt cacatcgatc tgaacatctt ttccgtgggt gacagtgcca cgaagatcaa
     2101 aacggcttgg gtcgataata gttacaccag ccaataacag tttttccgcc tcacgttttt
     2161 ggtagaaccg ttcaagtgca gcaagttgca aacggttgtt tgccccttct acttccataa
     2221 attcagttgc ctgaaccgct tgtactttaa agccttcttg gtttgccatt gcgatcacat
     2281 cagtgatgta atattcacct tgagcattgt tgttatttag gtttttcaac cattttttga
     2341 agctcgcacc gcttgctacc atcacgccgg tattcacttc tttgacttta agctgtgctt
     2401 cattggcatc tttttgctca acaatcgcta ccacagaacc attttcacga ataattcgtc
     2461 cgtagcctgt cgggttttcc aattcaacgg ttagtaatgc aataccattt tccggtttgg
     2521 ctgcgattaa acgctctaaa gtggcttttg tgattagggg ggcatcacca taaagcatta
     2581 caatgttttc atcatcagcg aaaaaaggag ctgcttgttg cattgcatgt cctgtgccta
     2641 attgttcggc ttggaatacc cagtttacag gctcttcgct taaacgtgct tttaacagat
     2701 cggcaccgtg tccgtaaatt aaatgaattt gctgggcatc tagctgtttg gcagtatcaa
     2761 taacgtgttt taccatgggt ttaccggcaa ctgggtgtaa cactttgggt aaatctgaat
     2821 acatacgagt gcctttaccg gcagctaaaa taacaacgct cagttgagtc atatagaatc
     2881 cttatttgac taaatagatg aaggcttata g
//
LOCUS       DANLYI010000227         2905 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.229, whole genome shotgun sequence.
ACCESSION   DANLYI010000227 DANLYI010000000
VERSION     DANLYI010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2905
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.229"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            70..2109
                     /gene="malQ"
                     /locus_tag="PXD81_002304"
     CDS             70..2109
                     /gene="malQ"
                     /locus_tag="PXD81_002304"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HDL5538285.1"
                     /translation="MSNKQNTAHYFYSPNIKEYASPSAFEKIERLLNTDNDLQKNNQI
                     LPLVKVITQPNAEQQAVEISLNFTNVAKWQIVLENGEVRNGEIKAEQSVLTLPADLPL
                     GYHQLSISTENDEESCSLIVTPPKAFQPKPLEERKKLWGAFLQLYTLRSDNNWGIGDF
                     GDLKQFLANISDYGADFVGLNPIHALFPADPDAASPYSPSFRLWQNIIYIDVSAVEAF
                     QQSEQAQQWFHTKETQQQLAELRAKDFVDYSQVAQLKLKALQFAFEHFSQSSQQQSQT
                     AFDAFIEQAGEPLKIQATFDALHHWISSEFGEKGGWTNWAEEYQSYYSPKVAEFQISQ
                     ATQIRFYMWLQFVANQQLAECNRFAQSLSMPIGFYRDLAVGVTQSGAETWADKALFVQ
                     EASIGAPPDMLAPNGQSWGLSPMHPHILQSRGYQPFIDLVRANMKHCGALRIDHILGF
                     ARMWWVANGESAKEGIYVRYPLEDLLSILALESQRHQCLVIAEALGIVPEGILDSLEQ
                     KGIFAYNVFYFEKAENAFKPLENYPYQAMTTLSTHDLPTIQGYWKGYDFELGEKFDTY
                     PSKAVLRKLKEERSYDRLNIRNAVEKVIALEPNEVGVTIKFTHQLQRYVAHTHSALFG
                     TQPEDWLNMLEPVNIPGTSTEYPNWRRKLSATTEQIFANKDIQQLLKDINEIRKG"
     gene            complement(2177..>2905)
                     /locus_tag="PXD81_002305"
     CDS             complement(2177..>2905)
                     /locus_tag="PXD81_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HDL5538286.1"
                     /translation="GCSSFSTKLKQEFFSGWTTFEASWLIVFLAIQIGIFIYQPDTWI
                     ATIAAITGILCVVFVGKGKISNYLFGLISVSLYAYISYTFQLYGEMMLNLLVYVPVQF
                     IGFYFWRKNMTSENTVNNAGVEEVIAKALTAKQWVIVAITTIIGTFLYIELLKYLGSA
                     LAILDGATVVISIVAQILMVLRYREQWALWIIVNIMTISLWTAMYFQNGETSLPLLVM
                     YVMYLCNSIYGYYNWIKLHRKHQQ"
BASE COUNT          929 a          616 c          571 g          789 t
ORIGIN      
        1 ctcttcttac tcatattaat cttcacaaga tcgttatttt aacaaataca gtaaaggaaa
       61 aaagtcataa tgtcaaacaa acaaaataca gctcactatt tttactcccc aaatattaaa
      121 gagtatgcca gcccctctgc ttttgagaaa atagagcggc ttttaaatac cgataacgat
      181 ttgcaaaaaa ataatcaaat tctaccgctt gttaaagtga ttactcagcc aaatgccgag
      241 caacaagcgg tcgaaatttc gctgaatttt acaaatgtag caaagtggca aattgtgctt
      301 gaaaatggtg aggttcggaa cggtgaaatt aaggcagagc aatcagttct aaccctgcct
      361 gccgatttgc ctttaggcta tcatcagctt tctatttcaa ctgaaaatga tgaagaatct
      421 tgctcgctga ttgtaacacc gccgaaagct ttccagccta agccattgga agaaaggaaa
      481 aagctctggg gtgcattttt acagctttat actttgcgtt ccgataacaa ctggggaatt
      541 ggcgattttg gcgatctcaa acaattcctt gccaacattt ctgattatgg tgcagatttt
      601 gtgggcttaa accctattca cgccctcttt ccggcagatc ctgatgcggc aagcccttac
      661 agcccgtcct ttcgactttg gcaaaatatt atttatattg atgtttcagc agtagaagcc
      721 tttcaacaat ctgaacaggc tcaacaatgg tttcacacca aagaaactca acagcaactt
      781 gccgaattgc gagccaaaga ttttgtcgat tacagccaag tagcgcaact gaaattaaag
      841 gctctgcagt ttgcttttga gcatttttca cagtcatctc aacaacaatc gcaaacagcg
      901 ttcgatgcct ttattgagca agcaggcgag ccattaaaaa tccaagcaac gttcgatgct
      961 ttgcaccact ggatttcatc tgaatttggc gaaaaaggcg gctggacaaa ctgggcggag
     1021 gaatatcaaa gttactattc gcctaaagta gctgaatttc aaatatcgca ggcaacgcag
     1081 atacgttttt atatgtggtt gcaatttgtg gcaaaccagc aattagcaga atgcaaccgg
     1141 tttgctcaat cactttcaat gccgattggt ttttaccgcg atttagccgt tggtgttact
     1201 caaagcggag cggaaacttg ggcggataaa gccctatttg tgcaagaggc gtcaatcggt
     1261 gcaccacctg atatgcttgc cccgaacgga cagagctggg gactttcgcc aatgcacccg
     1321 catattctac aaagccgagg ttatcagccg tttattgatt tagtgcgtgc taatatgaaa
     1381 cattgtgggg cattgcgaat cgaccatatt ctcggcttcg cccgtatgtg gtgggtggcg
     1441 aacggtgaat cggcaaaaga agggatttat gtccgttatc cgctcgaaga tttgctctct
     1501 attttggcat tagaaagcca acgtcaccaa tgtttagtga ttgcagaggc acttggcatt
     1561 gtgcctgaag gcattttgga ctctctcgag caaaagggca tttttgccta caatgttttt
     1621 tattttgaaa aggcagaaaa cgcgtttaaa ccgctggaaa attatcctta ccaagcgatg
     1681 acaactctaa gcacccacga tttaccgact attcaaggct attggaaagg ctatgatttt
     1741 gagctaggcg aaaaatttga cacttaccca agcaaagccg tattgagaaa gctgaaagaa
     1801 gagcgttcgt acgaccgctt gaatatccgt aatgcggtgg aaaaagtgat agcactcgag
     1861 ccaaatgaag tgggagtaac aataaaattt acccaccaat tacaacgcta tgtggctcac
     1921 actcacagtg cattattcgg tacacagccc gaagattggc tcaatatgtt agagccggtg
     1981 aacattccgg gaaccagcac cgaataccct aactggagaa gaaaattatc cgcaaccacc
     2041 gagcagattt ttgcaaataa agatattcaa caactgctaa aagatataaa cgaaattcgt
     2101 aaaggataat aacaagcggt catttttagg taaattttta caaaaaatga ccgcttgtaa
     2161 tttgtattta ttcgctctat tgctgatgtt ttctgtgaag tttaatccag ttgtaataac
     2221 cgtaaataga gttacataaa tacatcacat acatcaccaa cagcggcaag ctggtttcgc
     2281 cgttttggaa gtacattgcc gtccataaag aaatggtcat aatattcacg ataatccaca
     2341 atgcccattg ctcacggtag cgaagtacca tcaaaatttg tgccacaatc gaaataacca
     2401 ctgttgcacc atctaatata gctaacgcac tgcctaaata tttaagcaat tcaatataaa
     2461 gaaatgtgcc gataatggtg gtaatggcga caatcaccca ctgttttgcg gttaaagctt
     2521 tagcaattac ctcttccaca ccagcattat ttaccgtgtt ttcgctggtc atatttttac
     2581 gccagaaata gaaaccgata aactgcaccg gcacatatac aagtaaattc agcatcattt
     2641 caccatataa ctgaaaggta taagagatat aagcataaag cgaaacggaa attaagccga
     2701 atagatagtt actgattttt cctttaccga caaataccac gcataaaata cctgttattg
     2761 ccgcaatagt tgcaatccaa gtatcgggtt gataaataaa aataccgatt tgaatcgcta
     2821 aaaaaacaat caaccaactt gcctcaaaag tagtccaacc actaaaaaac tcttgtttta
     2881 acttagttga aaaagatgaa cagcc
//
LOCUS       DANLYI010000228         2865 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.230, whole genome shotgun sequence.
ACCESSION   DANLYI010000228 DANLYI010000000
VERSION     DANLYI010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2865)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2865)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2865
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.230"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            <1..878
                     /locus_tag="PXD81_002306"
     CDS             <1..878
                     /locus_tag="PXD81_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lysine exporter LysO family protein"
                     /protein_id="HDL5538287.1"
                     /translation="FLGYLITLKNHRHMAIVNRVVSLCLYLILLVMGISLGQIDNILT
                     ELPQIGGTAFSLVVILLLSNIIGLVIYDKLQPMKRDKVNQDQLPSRLHLLIDSFKLIG
                     ITLLGGIIGYFTKGVVDFPLHASTYVLEVMIFGVGIQLRNSGIPLREVFFNKRGIYTS
                     IVMVASSLIGGVISAFTLDLSLAKGLTFASAFGWYSLSSVLVNDAWGPIYGSIAFFND
                     ISREILCLFLVPLFMRQFPSTAVGIGGATSLDCMLPIIQKSGGIQIVPLAISFGFIVN
                     LAAPLLLAVFIGLEG"
     gene            1033..2799
                     /gene="aspS"
                     /locus_tag="PXD81_002307"
     CDS             1033..2799
                     /gene="aspS"
                     /locus_tag="PXD81_002307"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HDL5538288.1"
                     /translation="MMRSHYCGALNRSHLGQTVTLSGWVHRVRNLGRFIFMQIRDREG
                     IVQVFFDEKDEALFKQASALRSEACVQIQGEVIARDESQINKEMATGEIEVLVKNLVV
                     YNNAEVLPLDFNQNNTEEQRLKYRYLDLRRPEMAEKLKTRAKITSFVRRFMDDNGFLD
                     IETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFR
                     DEDLRADRQPEFTQIDVETSFMTAEEVRALMEAMIHGLWKETLGVDLGKFPMMTWNEA
                     MTRFGSDKPDLRNPLEMVDVADILKSVDFKVFSGPANDPKGRVVALRVPNGAALTRKN
                     IDDYTQFVGIYGAKGLAWLKVNDMNTGLEGIQSPVAKFLNEEVLKALVKRLNLQKDDI
                     VFFGADNWHVATNAIGALRLKVGNDLGLTDTTAWKPLWVIDFPMFERDDEGNLSAMHH
                     PFTSPKELSPEELVQDPENAVANAYDMVINGYEVGGGSVRIFSQQMQQTVFSILGINE
                     EEQKEKFGFLLDALKFGTPPHAGLAFGLDRLTMLITGTDNIRDVIAFPKTTAAACLMT
                     EAPSYANPKALEELAIRTIPQE"
BASE COUNT          789 a          595 c          654 g          827 t
ORIGIN      
        1 tttttctcgg ttacttaata accctcaaaa accaccgcca tatggcaata gtaaacagag
       61 tggtcagcct ctgcctttac ttgattttat tagtgatggg gatttctctc ggacaaattg
      121 ataacattct caccgaactg ccacagattg gcggtacggc gtttagttta gtggtgattt
      181 tactcctttc aaacattatc ggcttggtga tttacgacaa attacaaccg atgaaaagag
      241 acaaagtcaa tcaagatcaa cttccctccc gcctgcattt gttgattgat tcctttaaat
      301 taatcggcat taccttatta ggcgggatca tcggctattt caccaaaggc gtggtggatt
      361 tccctctgca cgccagcacc tatgtgttag aagtgatgat tttcggggtg gggattcaac
      421 tgcgaaacag cggtattccc ctgcgtgaag tgttctttaa caagcggggg atttatacct
      481 cgattgtaat ggtggcaagt tccctgattg gtggcgtgat ttcggcattc accttagatt
      541 tatcccttgc taaaggctta acctttgcct ctgcattcgg ttggtattcc ctttcaagcg
      601 tattggtaaa cgatgcttgg ggcccgattt acggcagtat tgcctttttt aacgacatct
      661 cgcgtgaaat tttatgcctg tttttagtac cactgtttat gcgtcaattt ccgtccaccg
      721 cagtcggcat tggcggagcg acttcattag attgtatgct ccctattatt caaaaatcgg
      781 gcggtattca aattgtgccg ctggcaatca gcttcggttt tattgttaat ttagccgctc
      841 cattgttatt agccgttttt attgggctgg aaggataaat cacaagcggt gatttttatc
      901 ccactttttg caaaaaatgg aataaaaatc accgcttgtt tgcttttatt tatccatctt
      961 cgctgaattt tccgctataa tccttaagtt ttccccgaaa cttggggtat ttattttcaa
     1021 aaggataatt ttatgatgcg ttctcactac tgcggtgcat taaaccgctc acaccttgga
     1081 caaacagtta ccttaagcgg ttgggttcat cgtgttcgta accttggtcg ttttattttt
     1141 atgcaaattc gtgatcgtga aggcattgtg caggtgtttt tcgatgagaa agatgaagca
     1201 cttttcaagc aagcatcggc cttacgttct gaggcgtgcg tgcaaattca aggtgaagtg
     1261 attgcccgtg atgaatcgca aatcaataaa gagatggcaa ccggtgaaat tgaagtgtta
     1321 gtaaaaaatt tagtggtgta taacaacgct gaagtattgc cgttagactt caatcaaaac
     1381 aataccgaag aacagcgttt aaaataccgc tatttggatt tacgccgccc tgaaatggca
     1441 gaaaagctca aaacccgtgc taaaatcacc agttttgtcc gccgttttat ggacgataac
     1501 ggtttcttag acattgaaac cccgatgctg accaaagcta ctcctgaagg tgcgagagat
     1561 tacctcgtgc caagccgtgt gcataaaggc aaattctatg ctctgccaca atcacctcag
     1621 ctatttaaac agttgctaat gatgtcgggc ttcgaccgtt attaccaaat cgtgaaatgt
     1681 ttccgtgatg aagatttgcg tgccgaccgc cagccggaat ttacccaaat agacgtggaa
     1741 accagcttta tgactgccga agaagtgcgt gcattaatgg aagcgatgat ccacggctta
     1801 tggaaagaga ccttaggcgt tgatctcggt aaattcccaa tgatgacgtg gaatgaagca
     1861 atgacccgtt tcggttcaga caaaccggat ttgcgtaacc cactggaaat ggttgatgtg
     1921 gcagatattt taaaatcggt tgatttcaaa gtatttagcg gtccggcgaa tgatccgaaa
     1981 gggcgtgtag tggcacttcg tgtgccaaac ggtgcagctc taacccgtaa aaatattgat
     2041 gactacaccc aatttgtcgg catttatggg gcaaaaggct tggcttggtt aaaagtgaat
     2101 gatatgaaca caggtttaga aggcattcaa agcccggttg caaaattctt aaatgaagaa
     2161 gtgctaaaag ccttagttaa acgcttaaac ttgcaaaaag acgacattgt attcttcggt
     2221 gcggataact ggcacgttgc gactaacgcc atcggtgcgt tacgcttaaa agtggggaac
     2281 gatttaggct taaccgatac cacagcgtgg aaaccgttat gggtgattga tttcccaatg
     2341 tttgaacgtg atgatgaagg caacttatcg gcaatgcacc acccgttcac ttcaccgaaa
     2401 gaattatcgc cggaagaatt agtgcaagat ccggaaaatg cggtggcaaa cgcttacgat
     2461 atggtaatta acggctacga agtgggcggt ggctcggtac gtattttcag ccagcagatg
     2521 caacaaaccg tgttcagcat tttaggcatt aacgaagaag agcaaaaaga gaaattcggt
     2581 ttcttattag atgcgttaaa attcggtact ccgccacacg caggcttagc cttcggttta
     2641 gaccgcttaa cgatgttgat tacaggaact gataacattc gtgatgtcat cgctttcccg
     2701 aaaaccaccg ctgcagcgtg tttaatgaca gaagcaccaa gctatgcgaa tccgaaagcg
     2761 ttggaagagt tagcaatccg tacaattcca caagagtaat cggtaggggc gaaacatttt
     2821 tcgccctttt tatttaacaa gcggtcgaaa aagtgaaaaa ctttg
//
LOCUS       DANLYI010000229         2857 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.231, whole genome shotgun sequence.
ACCESSION   DANLYI010000229 DANLYI010000000
VERSION     DANLYI010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2857
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.231"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            complement(<1..681)
                     /locus_tag="PXD81_002308"
     CDS             complement(<1..681)
                     /locus_tag="PXD81_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraI domain-containing protein"
                     /protein_id="HDL5538289.1"
                     /translation="MRSMFKKFFGKNKSQSAVKSDTIFSIEQDNEGWLYPEKASQLLN
                     TELRQKYLGLLWQQVSMNRQMFEVLYQQPIERYAEMVQLLPASESHHHSHIGGMLDHG
                     LEVIAFSAKVRQSYILPPNVAPEEQSKQRDAWTAAAIYLALVHDIGKVVVDIEITLKD
                     GSRWFAWNGQPPQPYKFKYIKGRDYHLHPVLGGFLANQLIPKSAFDWLGQYPEVISSL
                     MYAMSGHND"
     gene            complement(785..1249)
                     /locus_tag="PXD81_002309"
     CDS             complement(785..1249)
                     /locus_tag="PXD81_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArdC family protein"
                     /protein_id="HDL5538290.1"
                     /translation="MMNTQQPKFDLYQHITDRIIAALEQGTAPWLKPWSHPECNMALP
                     RNAVSNRLYSGINTLLLWIATAEHGYKQCKWITAKKANELGGYIRKGEKATVVMNYRP
                     NEREKLDEEGNIVYDEEGNPEMEQHFFIAKHNLFNLDQCENLPAELYDPVIP"
     gene            1458..1721
                     /locus_tag="PXD81_002310"
     CDS             1458..1721
                     /locus_tag="PXD81_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme-like superfamily protein"
                     /protein_id="HDL5538291.1"
                     /translation="MRPVTRNNRGTSFDPRHNKYINTLATALLARWHIEQFSERNIPV
                     NDENLYMAHNIGLDGFHRALLGQSTRKDIENMRKNGMKPWMSI"
     gene            complement(1805..2830)
                     /locus_tag="PXD81_002311"
     CDS             complement(1805..2830)
                     /locus_tag="PXD81_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03752 family integrating conjugative element
                     protein"
                     /protein_id="HDL5538292.1"
                     /translation="MTAGDTPHDTLKTLVGALKDTREEARKANERGDRFEQEVLRLQA
                     REAEVDSRIAGEVESRVESRLAAAWDGFNRQIEELKGRVQSGNQQQKEAEAIPVGGAS
                     SGFGTESAGQTGNVDNNGIRWVDPDDMVVTDEQGKPVTGNYSGKTKTGFPSPFKAEGE
                     SSNTGTALNNNAGTENMTGNTQIQQIKRTPYYTIPENSTLMGSTAMTALLGRVPIQGA
                     VTDPFPFKVLIGRENLIANGIDLPDVEGAIVSGTSTGDWTLSCVRSEVTSITFVFADG
                     RISNGRASGESRAVKRNQASNLTPFCLTVSLSGFNDIPHSDGNPGFLTMSAVDYVDVY
                     LMSHLAR"
BASE COUNT          796 a          642 c          557 g          862 t
ORIGIN      
        1 atcgttatgc ccagacattg catacatcag tgaagaaatg acttctggat actgtcccag
       61 ccaatcaaac gcagacttgg gaatgagctg atttgcaagg aatcctccta gcacgggatg
      121 aagatggtag tctcgacctt tgatgtattt gaatttataa ggttgaggtg gctgtccatt
      181 ccaagcaaac caacgagagc cgtctttaag agtgatttca atatcaacca caactttgcc
      241 aatatcatga accaaggcaa gataaattgc ggcagcagtc caagcatctc gttgctttga
      301 ttgttcttcg ggagctacat tcgggggcaa aatatagctt tgacgaactt tagctgaaaa
      361 agcaataact tctaaaccgt gatcaagcat tccaccgata tgtgaatgat ggtgtgattc
      421 agaagctggt aatagttgaa ccatctcagc gtaacgttct attggctgct gataaagtac
      481 ctcaaacatt tgacggttca ttgatacctg ttgccaaagt agcccaagat atttttgtcg
      541 taattctgta ttaagtaatt gagaggcttt ttccgggtat aaccaccctt cattatcttg
      601 ctcaatagaa aaaatagtat cggatttgac cgcactttga gatttatttt taccaaaaaa
      661 ttttttgaac attgaacgca ttggaagaga ccgaaaatat gaatgctgta ttgaatgaaa
      721 gccgtttagc cctttgccct tttgggtaag acctttcccc ttggaaaggt tactcttatc
      781 ccccttaggg gattacagga tcgtacagtt ccgccggtaa attttcacac tggtctaaat
      841 tgaacagatt gtgcttagca atgaaaaagt gttgttccat ttccggattt ccttcctcgt
      901 catacacaat atttccctcc tcatcgagtt tttctctctc attaggacgg tagttcatca
      961 caacagtagc tttttcccct ttacggatat atccgccgag ttcattggct tttttcgccg
     1021 taatccactt acactgtttg tagccgtgtt cagcggtagc aatccaaagc aataacgtat
     1081 tgataccgga atacagacgg ttagaaaccg cattgcgagg taatgccata ttgcattccg
     1141 gatggctcca cggttttaac cacggagctg taccttgttc caatgcagca atgattctat
     1201 cagtaatatg ttggtataaa tcaaatttag gttgttgagt gttcatcatg tgtttctcca
     1261 aagtttgggt aaaaaaatgg ggaaacacgg ttaccctatg gggaacttgt ttccccgatg
     1321 ggtagtttat aaaatttcaa tggccacaat ttttctagaa tcatcaaagc caacagaact
     1381 gcctatggcg tagtaattac gaatagtctg ttcatcggca ttgattagca gatacctatc
     1441 gaggaagaga gattgggatg agaccggtaa cgagaaataa tcgtggaacc agttttgacc
     1501 ctcgtcacaa taaatacatc aatacattgg caaccgcatt attggcacgt tggcatattg
     1561 agcaattctc tgagcgtaat atcccagtca atgatgaaaa tctctatatg gctcacaata
     1621 ttggcttaga tggtttccat cgagcgttat tagggcaatc tactcgtaaa gacattgaga
     1681 atatgcgtaa gaatgggatg aaaccttgga tgtccattta gctctgtttt caaatgacct
     1741 aatccagttt gttccggaga gagtaccgaa attttgcact ctttcatccg tggtgtatca
     1801 aacttcaacg tgctaaatga gacattaaat atacatcaac ataatctacc gcactcatcg
     1861 ttaagaaacc gggattccca tcagaatgag gaatatcgtt aaagcccgat aaggagacgg
     1921 ttaaacaaaa tggtgttaaa ttacttgctt gatttcgttt cacagctctg ctttctccac
     1981 tcgctcgccc attcgaaata cgaccgtctg caaacacaaa ggtaatgctc gttacctcag
     2041 aacgaacgca agatagcgtc caatctccgg ttgatgtacc ggatacaatc gccccctcaa
     2101 catcaggtaa atcaatgcca ttggcaatta aattttctcg cccgattaat actttaaacg
     2161 gaaacggatc tgtcacagca ccttgaatag gtacacggcc taacaatgcc gtcatcgcag
     2221 tagaacccat taaagtcgaa ttttcgggaa tagtgtaata aggtgtacgc tttatttgct
     2281 gtatctgagt attaccagtc atattttctg tcccagcatt attatttaat gctgtcccag
     2341 tatttgaact ttccccctct gctttaaaag ggcttggaaa gccggttttt gttttcccgg
     2401 aataattgcc tgtaaccggt ttaccttgtt catcggttac caccatatca tcaggatcga
     2461 cccagcgaat gccattgtta tctacattcc ctgtttgtcc tgctgattca gtcccaaagc
     2521 cagaagaagc ccctcctaca gggattgcct ctgcttcttt ctgctgctga ttaccacttt
     2581 gaacccgacc tttcagttct tcgatttggc gattaaatcc atcccaagca gcagccaaac
     2641 ggctttctac acgactttct acttcacccg caatacgaga atccacttcg gcttctcttg
     2701 cttgtaagcg taatacctct tgctcaaagc gatcaccacg ctcatttgct ttacgagctt
     2761 cttcacgagt atcttttaat gctcctacca acgtttttaa tgtatcgtga ggcgtatctc
     2821 ctgcagtaat acctaatgta ttcgcctcct catcact
//
LOCUS       DANLYI010000230         2837 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.232, whole genome shotgun sequence.
ACCESSION   DANLYI010000230 DANLYI010000000
VERSION     DANLYI010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2837)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2837
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.232"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            <1..508
                     /locus_tag="PXD81_002312"
     CDS             <1..508
                     /locus_tag="PXD81_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5538293.1"
                     /translation="AFTALILFLAIVFPENRYVEHALEPVINLLSGTGKVSSSTDNLV
                     KNHLFLPTLNQFISGDGLYYTETGGYYGGSDSGYIRQVLYGGVSYMLACFAFTYYFVR
                     KVAVNWFEGSWIFTLSTIGILAILNIKADTFAFPGLMMLFLMFLSLFGTSGRNVTFFC
                     KNKEAEYV"
     gene            501..1466
                     /locus_tag="PXD81_002313"
     CDS             501..1466
                     /locus_tag="PXD81_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005578999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDL5538294.1"
                     /translation="MFSIIVPSYNRNQEINALLESLKQQTAYNFEVIIVDDCSKIPVS
                     ETLGILNEFNDISTKEKVRELLTRDSLSHIYSYPFDVKVVRNNPNVGAAQSRNVGATN
                     ATQEWLLFLDDDDRFMPEKCERLAAEIANNPTANFVYHPAKCEMVNEGFTYVTKPFKH
                     KSELTKENILKANKIGGMPMIGLKKSLFCKIGGLSDRLRSLEDYDFLLKLLEEPSFEP
                     LYVDEALTYCTFHTKRSSVSTDTTNTEKAIEYIQQTYIKTPEANNFKLNSLYILSYPH
                     VMNLSRKAAGYYFEMFKQTKNIKQLIIALVIFISPKLAINLKRFM"
     gene            1466..2266
                     /locus_tag="PXD81_002314"
     CDS             1466..2266
                     /locus_tag="PXD81_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_162680708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDL5538295.1"
                     /translation="MKFSVLMSLYIKEQPQYLRECFESLKVQTVQADEIVVCFDGAVT
                     PELEAIVEEYSAILPINAVKFPQNRGLGKTLNDGLTYCKNEWVFRMDTDDICMPERFE
                     KQVAFIEQNPETVIFGGQIAEFGQDIHDLVAYRNVPTTVEEIVRFTRLRCPFNHMTVA
                     YQKSKVLEVGGYQDLQEDYYLWIKLIAQFKQVANLPDLLVYARVGNGMVGRRRGMAQA
                     EAEWRLYKLKNRCQIHDPISGFGIFIMRAIPRLLPVSALKKVYGLLRK"
     gene            complement(2303..2809)
                     /gene="msrA"
                     /locus_tag="PXD81_002315"
     CDS             complement(2303..2809)
                     /gene="msrA"
                     /locus_tag="PXD81_002315"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HDL5538296.1"
                     /translation="MQNIREIYLAGGCFWGVESFMQRIKGVIKAQSGYANGNTDNPSY
                     QEVCAGSGHAEVVKVTYDANQISLAKLLDYFFKVIDPVSINKQGGDIGIQYRTGIYYT
                     DPQDEPVVTAALAQLQTQFDQPLAVENQMLEQYFPAEEYHQEYLVKNPNGYCHIDIQL
                     MNEILKNQ"
BASE COUNT          838 a          501 c          592 g          906 t
ORIGIN      
        1 tgccttcact gcgttaattc tatttttggc gattgttttt ccggaaaacc gttatgttga
       61 acacgcctta gaaccggtaa ttaatttatt gtccggcacc ggaaaagtca gctcttcaac
      121 cgataattta gtgaaaaacc atctgttttt accgacttta aatcagttta tttccggtga
      181 tggtttatat tacaccgaaa ctggtggcta ttatggtgga tcggattccg gctatattcg
      241 ccaagtatta tatggtggag taagttatat gttggcgtgt tttgccttca cttactattt
      301 tgtacgcaaa gtggcggtga attggtttga aggcagttgg atttttacct tatccaccat
      361 cggcatttta gctattttaa atattaaagc agataccttt gctttcccgg ggctgatgat
      421 gctgtttcta atgttcttgt cgctgtttgg aacaagcggt cgaaatgtaa catttttttg
      481 caaaaacaaa gaggctgaat atgtttagta ttattgtgcc ttcttataac cgtaatcaag
      541 aaatcaatgc gttgcttgag agtttaaagc aacaaactgc atataatttt gaagtgatca
      601 ttgttgatga ctgctcaaaa ataccggtca gtgaaacttt aggaatttta aatgaattta
      661 acgatatttc tactaaagaa aaagtaagag aattacttac tagagatagt ttatctcata
      721 tttatagcta tccatttgat gtgaaagtgg tgcgaaataa cccaaatgtt ggggcggcac
      781 aaagccgaaa tgtaggggca actaatgcca cacaagaatg gctactgttt ttagatgatg
      841 atgaccgctt tatgcctgaa aaatgcgaac gtttagcggc agagattgcg aacaatccaa
      901 ctgccaattt tgtgtaccac ccggcaaaat gtgaaatggt aaatgagggt tttacttatg
      961 tcaccaagcc gtttaaacat aaaagtgagc taactaaaga aaacattcta aaagcgaata
     1021 aaattggcgg aatgccgatg attggcttga aaaaatcact cttttgtaaa attggtggat
     1081 tatctgaccg cttgcgatca cttgaagatt atgatttctt actgaaatta ttggaagaac
     1141 cgagttttga gccattgtat gtggacgaag ccttgaccta ttgtactttc cataccaaac
     1201 gctccagtgt ttcaaccgat accactaata cagaaaaggc tattgagtat attcaacaaa
     1261 cgtatatcaa aacacctgag gcgaataatt ttaagctcaa ttcgctttat attttaagtt
     1321 atccacacgt aatgaattta tcccgcaagg cagccggtta ttattttgag atgtttaaac
     1381 agactaaaaa tatcaaacaa ctgattattg cgttggttat ttttatttcg ccgaagttgg
     1441 cgattaattt gaaaaggttt atgtaatgaa attttccgtt ttaatgtcgt tatacattaa
     1501 agagcaaccg caatatttaa gagaatgttt tgaaagcttg aaagtacaaa ctgtgcaggc
     1561 tgatgaaatc gttgtttgtt ttgatggtgc agttacgcct gaattagagg cgattgtaga
     1621 agaatatagt gcaattttgc cgattaatgc ggtgaaattc ccacaaaatc gaggtttagg
     1681 taaaacgcta aatgatgggc taacctattg taagaatgaa tgggttttca gaatggatac
     1741 agacgatatt tgtatgccgg aacgtttcga gaaacaagtg gcatttattg aacagaatcc
     1801 tgaaaccgtg atttttggcg gccaaattgc tgaatttggg caagatattc acgatcttgt
     1861 cgcttaccgc aatgtaccaa ccacggtgga ggaaatcgtc agattcactc gcttgcgttg
     1921 cccgtttaac catatgacgg ttgcttatca gaaatccaaa gtgttggaag tcggtggcta
     1981 tcaagatctg caagaagatt actatttatg gatcaaactc attgctcaat ttaaacaagt
     2041 ggcaaacttg ccggatttat tagtgtatgc ccgagtcggt aacggaatgg ttggcagacg
     2101 ccgtggaatg gcccaagcag aggcagaatg gcgtttatat aaattaaaaa accgctgcca
     2161 gattcacgat ccgatttcag gttttgggat tttcataatg cgtgcaattc ctcgtttatt
     2221 accggtttca gcactgaaaa aagtgtacgg tttgctcaga aaataaagct aaaaaaacca
     2281 cagattgctc tgtggttttt tgttattgat tcttcaaaat ctcattcatt aattgaatat
     2341 caatatggca gtagccgttc gggtttttta ctaaatattc ttggtggtac tcttcggcag
     2401 ggaaatattg ctctaacatt tggttttcca ctgccagcgg ctgatcaaat tgggtttgta
     2461 attgtgcaag tgcggcggtt accaccggtt cgtcttgtgg gtcggtataa taaataccgg
     2521 tgcggtattg aataccgatg tcgccacctt gtttgttaat gctcaccggg tctattactt
     2581 taaagaagta atctagtaat tttgccaagc taatttgatt ggcatcataa gtcactttta
     2641 ccacttcagc gtgtccgcta ccggcacaca cctcttgata gcttgggtta tcggtattac
     2701 cgttggcata gcctgattgt gccttaatga cacctttaat ccgttgcata aaagattcca
     2761 cgccccagaa acagccaccg gctaagtaaa tttcacgaat attttgcata ttacctctct
     2821 ataattgtaa atttttc
//
LOCUS       DANLYI010000231         2815 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.233, whole genome shotgun sequence.
ACCESSION   DANLYI010000231 DANLYI010000000
VERSION     DANLYI010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2815
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.233"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            107..697
                     /locus_tag="PXD81_002316"
     CDS             107..697
                     /locus_tag="PXD81_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5538297.1"
                     /translation="MTTQITTAQINDLLKSHDIQGYANEWQGRRIYINLSAKNNESRG
                     DRNYQLYIDLATGELVSKLGKGLTTREFDAAVKAFENAFNAKDTAENTSPAESKETEQ
                     ENKQETTKLVITKPTLKGSEKQIAWADEIINLFIAKIGDTSIPVTATQEQITQAQAMV
                     DNFFAMTNAGKWIEKFKGLNDSSEPKQFWLSVKYGW"
     gene            767..1285
                     /locus_tag="PXD81_002317"
     CDS             767..1285
                     /locus_tag="PXD81_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5538298.1"
                     /translation="MTTQVTPQIMRIIGQIVAATYGDDVPTNVQTIILRYPIRGIGFI
                     SSRRELSINNGEIARLMDKIPGDLEDPKDGMPFDCQGAFWLGYYQYCKLSNDVKNYTS
                     KELSIIGESLYGTQWQSNLARDLRLSDARRVREWVAGERKIPFGVWADLTELVKAKKA
                     NLSSILKKLTID"
     gene            complement(1432..1671)
                     /locus_tag="PXD81_002318"
                     /pseudo
     CDS             complement(1432..1671)
                     /locus_tag="PXD81_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249173.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1832..2125)
                     /locus_tag="PXD81_002319"
     CDS             complement(1832..2125)
                     /locus_tag="PXD81_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5538299.1"
                     /translation="MTTFNKILNPMYSTIASYSTQDDGSLNAKYVVGTGDDTDGEVTN
                     FVIITSEYKYIDAQSAKAITDAPLTKEDIGKTPTQIMLGRIYKHLKETGQIVV"
     gene            complement(2240..2812)
                     /locus_tag="PXD81_002320"
     CDS             complement(2240..2812)
                     /locus_tag="PXD81_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009875117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5538300.1"
                     /translation="MNENFRSLSLQRKGRANFSNKIATIQAASDEIIVIKYSDGQVCL
                     LYRENGIATLATENATFAEYLIFSTNVPKSGGYGIEVYNSAGNVVFSSNHKFLRPIEY
                     VDLNPSKNVFRFTGQANRQYGIILSNFGFDFVINYDNGYSTSRSAKVSGNFIEFGFCK
                     YHYSGLYKTGMSYNSKAMYFNALVVDITGY"
BASE COUNT          927 a          533 c          522 g          833 t
ORIGIN      
        1 aaaaatttaa aaaaactctt gcaataaaat tattatgcgc ctataattag gctcataaag
       61 caaggctagg gagccagcta ataaccatca gccaaagggg gctaaaatga caactcaaat
      121 tacaaccgca caaatcaacg acttattaaa atctcacgat atccaaggct acgccaacga
      181 atggcaaggt cgccgcattt acattaacct ttcagcaaaa aataacgaaa gcagaggtga
      241 tcggaattat cagttgtaca ttgacttagc cacaggtgag ttagtgagca aacttggcaa
      301 aggattaaca acaagagagt tcgacgctgc cgtaaaagca tttgagaacg cttttaatgc
      361 taaagacacc gctgaaaata catcgccagc agaaagcaaa gaaacagagc aagagaacaa
      421 acaagaaaca actaagcttg taatcacaaa accaacatta aaaggctctg aaaaacaaat
      481 cgcttgggca gacgaaatca tcaatctttt tattgcgaag attggtgata cctctatccc
      541 agtaacagca acgcaagaac aaattactca agctcaagca atggtagata atttttttgc
      601 aatgacaaac gctggaaagt ggatcgaaaa atttaaaggt ctaaatgata gtagcgaacc
      661 aaagcaattt tggttatccg taaaatatgg atggtagatt gagataacaa ataagccgta
      721 agagcaaagg cttacggcta aaacaaacag ataactggag attattatga ctacacaagt
      781 aacaccacaa attatgcgca ttatcgggca aattgtcgct gctacttatg gtgacgatgt
      841 gccaacaaac gtacagacaa taatcttgag ataccctata agagggatag ggtttatctc
      901 tagtcgcaga gagctatcta ttaacaatgg agagatagca agattaatgg ataaaatacc
      961 aggcgacctc gaggacccta aagacgggat gccttttgat tgtcaggggg cgttttggct
     1021 tggatactat caatactgca aattaagtaa cgatgtaaaa aattacacat caaaagagct
     1081 atccatcatt ggcgagtcat tgtatggcac tcaatggcag tctaatctag cgagagattt
     1141 gagattatct gatgctcgcc gtgtccgcga atgggtggct ggtgaacgta aaatcccttt
     1201 cggggtttgg gctgatttga ccgaattggt taaagccaaa aaagctaact tatcatcaat
     1261 tcttaaaaaa ttaacaatag attagcactg actttaaagc ccctctcggg gcttaattat
     1321 agaaaataaa atcacaatgg ctaattttgc caaatttgcc tgttaaaacg tgcgaaaaat
     1381 tacaaaaaac ggctagagct atagccgttt tgagtgcaga tgttaacaga gttaagctct
     1441 agcacgtcct gggccttgat actgcacacc atcagttaaa tgatcttgat tttcggttgc
     1501 tgctacagca gattctgatc ttacatttaa actttgtgac tgttgtaatg catcaataag
     1561 tttatttacc actgttccga gtgttttaat ttgctcggtt tgctcattaa tagtctgctt
     1621 taactcttgc agttttaatg attgctcatc cacaaccact aatcgacggt tagctcaatt
     1681 tgaggctctg tgctgccctc tactatggcg tgtaaatcca gcctaatacc ctctaaattt
     1741 acaggctcgc catttgtttg cctaaaaaaa aggcgataac ttacgtcatc gcctctgaaa
     1801 aacttaaatt tatgcttttg catttagacc cctacactac aatctgcccc gtctctttta
     1861 agtgcttata gatacgacct aacataatct gcgttggcgt tttgccaata tcctcttttg
     1921 ttagcggtgc atctgtaatc gctttggctg attgagcatc aatatatttg tactcacttg
     1981 tgatgattac aaagtttgtt acctcgccat cggtatcatc acctgtgcca acaacatact
     2041 tagcattgag tgagccgtca tcttgagttg agtaacttgc gatagttgag tacattgggt
     2101 ttaagatttt gttaaaagtt gtcataattt ttccttttgt ggattgttga taaaaaaaga
     2161 ccgcttgtat tgctacaagc ggcgatgaat gtgtaatcag aattttagtt cgcctctatt
     2221 aaatgaaggc gtttaatgtt taatagcctg ttatatctac aaccagagca ttaaaataca
     2281 ttgcttttga attataactc attcccgtct tatacaaacc agaataatgg tatttacaga
     2341 acccaaattc aataaaatta ccacttactt tggcagatct agatgttgaa tatccgttat
     2401 cgtagttaat gacaaaatca aagccaaaat tggataagat aatgccatat tgcctatttg
     2461 cttgccctgt aaatctaaaa acattttttg atggatttag atcgacatat tcaataggtc
     2521 ttaaaaattt gtggtttgat gaaaatacaa catttcctgc tgagttataa acctctatgc
     2581 cgtaacctcc gcttttcggt acatttgtac taaaaatgag atattcagca aatgttgcgt
     2641 tttctgtagc taatgtggca ataccatttt ctcgatatag caaacatact tgcccatcag
     2701 aatatttaat gacaataatt tcatctgacg ctgcctgaat agttgcaatt ttattagaaa
     2761 agttagctcg tccttttctt tgtaaggata aacttctgaa attttcattg atgtt
//
LOCUS       DANLYI010000232         2815 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.234, whole genome shotgun sequence.
ACCESSION   DANLYI010000232 DANLYI010000000
VERSION     DANLYI010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2815
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.234"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            <1..>2815
                     /locus_tag="PXD81_002321"
     CDS             <1..>2815
                     /locus_tag="PXD81_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015587035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HDL5538301.1"
                     /translation="WEIYDLAPILSKMLGVEISFDKWALYDVARYCDQLVPDGMGGME
                     PRFTCNVWLTEVKTAYDLLNDFCSVFRAIPIWTGTEVSVIIDRPRDPVWTYTNANVVG
                     GFERSYSARKSRHNAVQVTYSDKTNGYESAIEYVSDDEEIKKHGLNLSQITAFGCTSR
                     GQAHRTGKWILETEKREKETITFTVGREGLMHLPGDIIRVADSHYAGTEIGGRVLAIN
                     GRKVTLDREISIDNASYFTYINGEATHSSKIQSVNGKEITLDSTPTGLETYGVWSLST
                     KQISSGLYRSISIVENADGTNTITALQHEPQKEAIVDNSAHFVETARTLYKAPQINAV
                     EVSTGYDGKLYITGDISSGDGKLTYDIKITKDGNLYQYKKGLSDPNIELSDLPNGDYT
                     VIIYGKNAKGQIVTEKTQTFTIDRPPAPTGVVVTGGLGQITLEWDWVNEVTQTEIFAA
                     ETDNFALAKKIAKVTARTYAHTLKGNKVVRYYWLRHTRGINVGPFYQQQGVRGQTAVD
                     LDARLTELNTQLSRNIVNEVFDVAAPARGLELVKTVANLTDKGTKLASSQVYNQADGK
                     LYTWNGTAYSATVAAEDVTGKLSKSKIDTSLISQLTGADNTANLARRLAETAQSNINQ
                     EITNRQDAVTAEANNRTKAIQAESANLTKKIQAEATARGAAVTQLQNVDAQQAQLISA
                     VTAKADNALSGLEEEKTARANGDKAESQAREALTARMGAAESNIVTIQRTVANNAQSI
                     SEVSQNLNAKIDNINIGGRNLLRDSEFNAYNKWGSPQIEFAENANRRTIKVTSTGTNG
                     PVGIVSSNRHSTSYFQQGETYTLSLFARGSKALDYLYLMRQDGNNVRLPVINVASETE
                     FNHYKLTFKAPFTTQQGYVLIGFRQTSTEQFVEFHSVKLEKGNVATDWTPAPEDVESA
                     VSAVSADLTSYKQTQAATDSAQA"
BASE COUNT          904 a          583 c          628 g          700 t
ORIGIN      
        1 cttgggaaat ttacgattta gctccgattt taagcaaaat gcttggggtt gaaatcagtt
       61 ttgacaaatg ggcgttgtat gatgttgctc gctattgcga tcaacttgtg ccggacggta
      121 tgggtggaat ggagcctcgt ttcacctgta acgtatggct cacggaagtt aaaactgcct
      181 atgacttgtt gaatgatttc tgctcggtat tccgtgcgat tccgatttgg accggaacag
      241 aagtatcggt cattatcgac cgtccgcgtg atccggtttg gacttacaca aatgctaatg
      301 tagttggtgg atttgaacga tcctactctg ctcgcaaatc acgccataat gcagtgcaag
      361 ttacttattc ggataaaaca aatggctatg aaagtgcgat tgaatatgtc tctgatgacg
      421 aggaaatcaa aaagcacggc ttaaatttaa gtcaaattac tgctttcggt tgtacctctc
      481 gggggcaagc acaccgtacc ggaaaatgga ttttggaaac cgaaaaacgg gaaaaggaaa
      541 ccattacttt taccgtggga cgtgagggct taatgcactt gccgggcgat attattcgtg
      601 tggcagacag ccattatgcc ggtacggaaa ttggcggacg tgtactggcg attaatgggc
      661 gaaaagtcac gttagatcgt gaaattagca ttgataacgc cagttatttt acctatatca
      721 acggtgaggc aacgcactct agcaaaatcc aatcggtaaa cggcaaagaa attacgctgg
      781 atagtacacc aaccggctta gaaacctacg gtgtttggtc gttatcgact aaacaaatca
      841 gctctggatt atatcgctca atctcgattg tcgaaaatgc ggacggcaca aacactatca
      901 cagcgttaca acacgaaccg cagaaagagg caattgttga taactccgct cactttgtgg
      961 aaacggctag aacactctac aaagctccac aaattaacgc agtggaagtc tcgaccggtt
     1021 atgacggtaa gctctacatt accggtgata ttagctccgg tgatggcaag ctaacctatg
     1081 atattaaaat taccaaagat ggcaatttat atcaatacaa aaaaggctta agcgatccga
     1141 acattgaact aagtgattta cctaacggcg attataccgt cattatttac ggcaaaaacg
     1201 ccaaagggca aatcgtcacc gaaaaaacac aaacctttac tattgatcga cctccagctc
     1261 cgaccggtgt agtagtaaca ggtggattag ggcaaattac cctcgaatgg gattgggtaa
     1321 atgaggttac gcaaaccgag attttcgcgg ccgaaaccga taatttcgcc cttgcaaaaa
     1381 aaatcgcaaa agtgaccgct agaacctacg ctcacacact aaaaggtaac aaggttgtac
     1441 gctattattg gctacgtcat accagaggca ttaatgtcgg accgttttat caacaacagg
     1501 gggtaagagg acaaacagcg gttgatttgg atgctcgatt aaccgaatta aacactcagt
     1561 tatcacgcaa tattgttaat gaggtttttg atgttgctgc accggcaaga ggtttagaat
     1621 tagtgaaaac agtcgctaat ttgaccgaca aaggtactaa actggcgtca tctcaagttt
     1681 acaatcaggc tgacggcaag ctctatactt ggaacggtac agcatactca gctacagtgg
     1741 ctgcggaaga tgttaccggc aaattatcaa aatctaaaat tgatacgtcc cttatttcgc
     1801 aattaacagg tgcagataac accgcaaatt tggcaagaag attagcggaa acagctcaaa
     1861 gcaacattaa ccaagagatt actaaccgac aagatgccgt tactgccgaa gcaaataacc
     1921 gtactaaagc cattcaagcc gaaagtgcga atttaacgaa aaaaattcaa gctgaagcaa
     1981 ccgctcgtgg ggctgctgtt acacagttac aaaatgttga tgctcaacaa gcacagttga
     2041 tttctgctgt cacagcaaaa gctgataatg ctctttcagg cttggaagag gaaaaaacag
     2101 cacgagcaaa cggcgataaa gcggagtctc aagcacgtga agcattaaca gcaagaatgg
     2161 gagcagctga aagcaatatc gtaactattc aacgtacggt agcaaataat gcccaaagca
     2221 ttagtgaagt cagtcagaat ttaaatgcta agattgataa tattaatatc ggcggtcgga
     2281 atttattaag agacagcgag ttcaatgcgt acaataaatg gggtagtcca caaattgaat
     2341 ttgctgaaaa tgcaaatcgc cgaactatca aagtaacctc aacaggtact aacggtcctg
     2401 ttggtattgt ttcatctaat cgccattcca catcttattt ccaacaagga gaaacttata
     2461 cactctcatt atttgctaga ggaagtaagg cactagacta tctctatcta atgcgtcaag
     2521 atgggaataa cgttagatta ccagtgatta acgttgcctc tgaaactgag tttaatcatt
     2581 acaaactcac gttcaaggct ccttttacca cgcaacaagg ttatgtctta atcggttttc
     2641 ggcaaaccag cacagagcag tttgttgagt ttcacagcgt caaacttgaa aaaggcaacg
     2701 tagccacaga ctggacacca gcaccggagg atgtggagag tgcggttagt gctgtttcgg
     2761 ctgatttaac gagttataaa caaactcaag cggctaccga ttcagcacaa gcaca
//
LOCUS       DANLYI010000233         2814 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.235, whole genome shotgun sequence.
ACCESSION   DANLYI010000233 DANLYI010000000
VERSION     DANLYI010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2814
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.235"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            366..656
                     /locus_tag="PXD81_002322"
     CDS             366..656
                     /locus_tag="PXD81_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HDL5538302.1"
                     /translation="MALRPLHDKVILKREEVETKSAGGIVLTGSAATKSTRGKVIAVG
                     TGRILDNGSVQPLAVKVGDVVIFNEGYGVKTEKIDGEEVLILAEHDILAIVE"
     gene            725..2365
                     /gene="groL"
                     /locus_tag="PXD81_002323"
     CDS             725..2365
                     /gene="groL"
                     /locus_tag="PXD81_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011608625.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HDL5538303.1"
                     /translation="MAAKDVKFGNDARVKMLKGVNILADAVKVTLGPKGRNVVLDRAY
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIVNE
                     GLKAVAAGMNPMDLKRGIDKAVAAVVEELKAISKPCETSKEIEQVGTISANSDSTVGQ
                     LIAQAMEKVGKEGVITVEDGTGLEDALDVVEGMQFDRGYLSPYFINKPEAGTVELENP
                     YILLVDKKVSNIRELLPVLEGVAKAGKPLLIIAEDIEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIAILTAGTVISEEIGMELEKATLEELGQAKRVVISKDNTTIID
                     GIGDETQIKGRVAQIRQQIEDSTSDYDKEKLQERVAKLAGGVAVIKVGAATEVAMKEK
                     KDRVDDALHATRAAVEEGIVPGGGVALVRAANKVADTLKGDNEEQNVGIKLALRAMEA
                     PLRQIVANAGEEASVVARNVKEGSGNYGYNAGTEQYGDMLEMGILDPTKVTRSALQFA
                     ASIAGLMITTECMITDLPKEEKLDPAAMGGMGGMGGMM"
     gene            complement(2490..2777)
                     /locus_tag="PXD81_002324"
     CDS             complement(2490..2777)
                     /locus_tag="PXD81_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5339 domain-containing protein"
                     /protein_id="HDL5538304.1"
                     /translation="MVSSNAIASVKTSSLTVAKPTLSKNLPTECEKMFNTANKLVSDA
                     ERQPGTHTQVLKMKNKLSLTKQQILKMDSELQQKSCEKGLTALNSLKQKHQ"
BASE COUNT          898 a          451 c          653 g          812 t
ORIGIN      
        1 gaaataaatt ttatctgatt caaccaaaat tgcttgtaca ggttcttcaa attcttttcc
       61 tctaaaatca taactttcgt taaagcatgc ggagagaaac catagtgagc agagcattaa
      121 gtattttttc attttattat cctatcttga aattaatagt ttaaatggta taagagaatt
      181 ggggtttttc taaacagaaa cagatttctt aggtatatag ttttatcaaa aatggtcaaa
      241 tttgtaaaaa attttacaaa tttgaccgct tgcacttgaa actctaaaat ctgcctttat
      301 attgttaggc gttacgcatt ttgcgtgaag ttttatttat tcatttttaa gaaaaggaac
      361 atattatggc acttcgtcca ttacacgata aagttatttt aaaacgtgaa gaagttgaaa
      421 caaaatcggc aggcggtatt gttttaaccg gttcggcagc aacaaaatca acccgtggca
      481 aagtaattgc agtaggaaca ggtcgcattt tagacaatgg ttctgttcag cctttagcag
      541 ttaaagtggg cgatgtagtg atttttaacg aaggctacgg cgtaaaaacc gagaaaattg
      601 acggcgaaga agtgctaatt ttagcagagc acgatatttt ggcgattgta gaataatttc
      661 gttctgcaca agcggttaaa aaagcaaaat tttttacata tttcaaatac aaaggattta
      721 gaaaatggca gcaaaagacg tgaaatttgg taacgatgca cgcgtaaaaa tgttaaaagg
      781 cgtgaatatt ttagccgatg cggtaaaagt aactttaggt ccgaaaggtc gtaatgtggt
      841 gttagatcgt gcttatggtg caccaacgat tacaaaagac ggtgtgtcgg ttgcccgtga
      901 aatcgaatta gaagataaat ttgaaaatat gggggctcaa atggtgaaag aggttgcctc
      961 taaagccaat gatgccgccg gtgacggtac aactaccgca actgtattag ctcaagcgat
     1021 tgtaaacgag ggcttaaaag cagtggctgc gggtatgaac ccaatggatt taaaacgtgg
     1081 tatcgataaa gcggttgcag cagtggttga agagttaaaa gcgatttcaa aaccgtgcga
     1141 aacctcaaaa gaaattgagc aagtagggac gatttctgca aactcagatt caacagtcgg
     1201 tcaattaatt gctcaagcaa tggaaaaagt gggcaaagag ggcgtgatta ctgttgaaga
     1261 cggtacaggt ttagaagatg cattagacgt ggttgagggt atgcaatttg accgtggtta
     1321 cttatcgcca tactttatca ataaaccgga agccggtacg gttgagttag aaaacccata
     1381 tattctttta gtggataaaa aagtgtctaa cattcgtgaa ttattaccgg tgttagaagg
     1441 tgttgcaaaa gcaggtaaac cgttattaat tattgccgaa gatattgaag gcgaggcgtt
     1501 agctacctta gtggtgaaca ctatgcgtgg tatcgtgaaa gtggcggcag tgaaagcacc
     1561 tggctttggt gatcgccgta aagcaatgtt acaagatatt gcgatcttaa ctgcaggtac
     1621 tgtgatttct gaagagatcg gtatggagct tgaaaaagca acccttgaag agcttggtca
     1681 agcaaaacgt gtggttatca gcaaagataa caccacaatt attgacggta ttggtgatga
     1741 aactcaaatc aaaggtcgtg tagcacaaat ccgccaacaa atcgaagatt caacttcaga
     1801 ttacgacaaa gaaaaactac aagagcgtgt agcaaaatta gccggtggtg ttgcggtaat
     1861 taaagtaggt gcggcaactg aagtggctat gaaagagaaa aaagaccgtg ttgatgatgc
     1921 tcttcacgca acccgtgctg cggtggaaga aggtattgta ccggggggcg gagttgcttt
     1981 agtgcgtgca gccaacaaag tggctgatac actaaaaggc gataacgaag agcaaaatgt
     2041 aggtatcaaa cttgccttgc gtgcaatgga ggctccatta cgccaaatcg ttgccaatgc
     2101 aggtgaagaa gcctcagttg tagcccgcaa cgtgaaagaa ggttcaggca actacggcta
     2161 taacgcaggt acagagcagt acggcgatat gcttgaaatg ggaattttag acccaactaa
     2221 agtgactcgt tcagcacttc aattcgcggc ttctattgca ggtttaatga tcactacaga
     2281 atgtatgatt accgatctgc caaaagaaga gaaattagat cctgccgcaa tgggcggtat
     2341 ggggggaatg ggcggaatga tgtaattgcc cattgagtga acaaaagcta ctgaaattaa
     2401 ttcagtagct tttttgttgg taacaagtgg ttaaatttat gctatttttt gcaaataaaa
     2461 aaccaagcaa gtaagcttgg ttttagcgtt tattgatgtt tttgtttaag gctgtttagt
     2521 gctgttaaac ctttctcaca acttttttgc tgtaattcag aatccatttt taggatttgt
     2581 tgctttgtta aagaaagttt atttttcatt tttagcactt gagtatgagt accaggctgt
     2641 ctttctgcat cagagaccaa tttatttgcc gtgttaaaca ttttctcaca ttcagttggc
     2701 aaatttttac ttaaagtagg ttttgcaact gtaagtgatg aggttttaac acttgctata
     2761 gcattactgg aaaccaatat ggtacaaagt gttattccta aaattctttt tagc
//
LOCUS       DANLYI010000234         2744 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.236, whole genome shotgun sequence.
ACCESSION   DANLYI010000234 DANLYI010000000
VERSION     DANLYI010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2744
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.236"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            complement(<1..1247)
                     /locus_tag="PXD81_002325"
     CDS             complement(<1..1247)
                     /locus_tag="PXD81_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HDL5538305.1"
                     /translation="MKTLTLTANKITAVEGAAIGANQGSFAQKLNSTKARIDQLNAYR
                     IERAINANNHHFMHVFSLIPLLIHLNHPVLPAYVENAPQGIWHFQLSDYQKQFLVSYR
                     FDEAISADHLGPHPSFDALYSMGSTGTITQTSCSDLDLWLCYPNHFTIEQYKLMELKI
                     NKLQEWAKTFQIDINIYLMNPEQFKSQTYNSSVTDEHSGSAQHFFLLDEFYRSAILLA
                     GKRLLWLHLHKNEYQNVHNNPEIDLTEWIDFGDFSSLSTSEFFGASLWQLYKGINNPY
                     KSAIKILLLECYAHTYPKTKLISKEFKKKLLSDNALEYHFDPYLAMLELVTEHLRSRK
                     EWVKLDRLRVCFYTKAVEGEINNNWRSRDLDELVNQWSWSEKQIQLLQNRQHWKIKQV
                     MNHENMLIDHLLQSYRNLIHFAR"
     gene            1508..2725
                     /gene="fabB"
                     /locus_tag="PXD81_002326"
     CDS             1508..2725
                     /gene="fabB"
                     /locus_tag="PXD81_002326"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HDL5538306.1"
                     /translation="MKRVVITGLGVISSIGNNKEEVLESLKAGKSGIEFVPEFAEVGM
                     RSQVAGTIKLNPAELIDRKIYRFMGDAAAYAYLSMKEAIEDSGLTEEQVSNDRTGLVI
                     GAGTGSAHSQLVACDAVRGPRGVKAVGPYAVTKTMASSVSACLATPYKIRGVNYSISS
                     ACATSAHCIGHAMELIQLGKQDVVFAGGAEELSWECATEFDAMGAVSTKYNETPTKAS
                     RAYDANRDGFVIAGGGAVVVVEELEHALARGAKIYAEIVGYGATSDGYDMVAPSGEGA
                     ERCMRQAMATVNGEIEYINVHGTSTPVGDVKELGAIRNVFGDKTPAISSTKSMTGHSL
                     GAAGAHEAIYSLLMLDNNFIAPSINIETLDEQAQGMNIVTERQDKALTTVMSNSFGFG
                     GTNACLVFQRYSK"
BASE COUNT          838 a          465 c          611 g          830 t
ORIGIN      
        1 tttcgagcaa aatgaattaa atttcgataa ctttgtagta aatgatcaat cagcatattc
       61 tcatgattca tcacttgctt aattttccaa tgttgacggt tttgaagtaa ttgaatttgt
      121 ttttctgacc aagaccattg gttaaccagc tcgtctaaat cacgacttct ccagttgtta
      181 tttatttctc cctcaaccgc ttttgtataa aaacagactc gcaagcggtc taatttcacc
      241 cactctttgc gtgatcttaa gtgttctgtt accaattcaa gcatggctaa gtagggatca
      301 aagtgatatt ccaaagcatt gtcacttagt agttttttct taaattcttt ggagatgagc
      361 tttgttttag gataagtgtg agcgtagcat tcgagcaata aaattttaat tgccgattta
      421 taagggttat taattccttt gtagagctgc cataagcttg caccaaaaaa ttcactggtt
      481 gaaaggctgg aaaaatcgcc gaaatcaatc cattccgtta agtcaatttc agggttattg
      541 tgaacatttt ggtattcatt tttatgtaaa tgcaaccaga gtaaacgctt accggcaagt
      601 aaaatagctg aacgataaaa ttcatctaat agaaaaaaat gctgtgctga accactgtgt
      661 tcatcggtta cgctactgtt ataagtttgg ctcttaaatt gttctgggtt cattaaataa
      721 atattgatgt cgatttggaa tgtctttgcc cattcttgca atttattaat tttgagctcc
      781 attaacttat attgttcaat tgtaaaatgg ttcggatagc atagccataa atctaaatca
      841 gaacagcttg tttgggtaat agtaccggtg ctacccatcg aataaagagc atcaaagctg
      901 ggatgggggc ccaaatgatc ggcagagatt gcttcatcaa aacggtaaga aaccagaaat
      961 tgtttttgat aatcagagag ttgaaaatgc caaatccctt gtggtgcatt ttcaacataa
     1021 gccggtaaca cagggtgatt aagatgaata agcaatggaa tgagagagaa aacatgcata
     1081 aaatgatgat tattagcatt gatagctcgt tctattcggt atgcattaag ttgatcaatt
     1141 cgtgctttag tagagtttaa tttttgggcg aagctcccct ggttcgcccc aatggcagcg
     1201 ccttctacgg ctgttatttt gttggctgtc agggttaatg ttttcacgtc agctttcctt
     1261 actaaagtgt gaactgttta gcattttagc ggaaaagtga ttattatgta atagggtaaa
     1321 aaatcgggaa attaaatttc attccctatt ttgcaaaaaa tgtctgaaat tcaaccgcta
     1381 tcttgataga aagagttcag accagtaatg aaattgtgta aaataatcaa gttttagtag
     1441 cttatttgag tgaacaagtg taagctgaat caagtatttt tcagatcaaa atgatgaagg
     1501 aaacaaaatg aaaagagtcg ttattacagg attaggtgta atttccagta tcggtaacaa
     1561 caaagaagaa gtgttggaat cactaaaagc aggtaaatca ggcattgaat ttgtgcctga
     1621 gtttgctgaa gtgggtatgc gtagccaagt ggcaggtacg attaaattaa atccggctga
     1681 gttaattgac cgtaaaatct atcgttttat gggagatgct gcggcttacg cttatctttc
     1741 aatgaaagaa gcaatcgaag actcaggctt aaccgaagaa caagtttcaa acgatcgtac
     1801 cggtttggtg atcggagctg gtacaggctc ggcgcacagt caacttgtag cttgtgatgc
     1861 agtacgtggc ccacgtggtg tgaaagccgt tgggccttac gcagtaacca aaacaatggc
     1921 atcaagtgtg tcagcatgct tggcaacacc ttataaaatt cgtggtgtaa actattcaat
     1981 cagctcagct tgtgcgactt cagcacactg tattggacac gcgatggagt taatccaatt
     2041 aggtaaacaa gatgtggtgt ttgcaggtgg tgcagaagaa ctttcttggg aatgtgcaac
     2101 cgagtttgat gcaatgggtg ctgtttcaac taaatataat gaaacaccaa caaaagctag
     2161 ccgtgcttat gatgctaatc gtgacggttt tgtgattgcc ggtggtggtg cagtggttgt
     2221 ggtggaagag ttagaacacg ctcttgctcg tggtgccaaa atctatgctg aaattgtggg
     2281 ctatggggca acctcagacg gttacgatat ggtcgcgcca agtggtgaag gtgctgagcg
     2341 ttgtatgaga caggcgatgg caactgtaaa tggtgaaatc gagtatatta acgtacacgg
     2401 cacttctact ccagtgggtg atgtaaaaga gttaggggcg atccgtaatg tctttggtga
     2461 taaaacgcca gcaatttcat caaccaaatc aatgaccgga cactcattag gtgcagcagg
     2521 tgcgcacgag gcaatttatt cattattaat gttagataat aattttattg caccaagcat
     2581 taatattgaa acccttgatg aacaagcaca aggtatgaat attgtgactg aacgccaaga
     2641 caaagcatta acaaccgtga tgtcaaacag cttcggcttt ggtggtacta atgcgtgttt
     2701 agtgttccaa cgctattcta agtaacataa aaagagcaag tgaa
//
LOCUS       DANLYI010000235         2729 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.237, whole genome shotgun sequence.
ACCESSION   DANLYI010000235 DANLYI010000000
VERSION     DANLYI010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2729
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.237"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            6..1073
                     /gene="hisC"
                     /locus_tag="PXD81_002327"
     CDS             6..1073
                     /gene="hisC"
                     /locus_tag="PXD81_002327"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HDL5538307.1"
                     /translation="MTISQLSRKNIQALTPYQSARRLGGSGDVWLNANEYALSPNFDL
                     TDRTFNRYPEPQPQAVIEGYARYAGVAPENVLVSRGGDESIELIIRAFCEANDSILYC
                     PPTYGMYAVSADTCGIATKTVPLTADFQLDLAQIKANLEGVKVVFVCSPNNPTGNLIK
                     RSDLFELLQITAGKAIVVVDEAYIEFCPEARLVSELANFPHLAIIRTLSKAFALAGLR
                     CGFTLANAELIGVLQKVIAPYPLPVPVSDIAAQALSPQGIEQMKTRVAEVITLRAELQ
                     KNLENLPLVEQVFDSEANYLLFKCQDGQKVFKALWEQGIILRDQHKALGLQNCIRITV
                     GAREENLRVVEAITALSQFQR"
     gene            1258..2346
                     /gene="hisB"
                     /locus_tag="PXD81_002328"
     CDS             1258..2346
                     /gene="hisB"
                     /locus_tag="PXD81_002328"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018355873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HDL5538308.1"
                     /translation="MQPTLFIDRDGTLIDEPKTDFQIDSLEKLKFEKNVIPALLKLKG
                     KYRFVMVTNQDGLGTSSFPQADFDKPHNAMMALFNSQGIEFDDVLICPHKPEDGCDCR
                     KPHTKLLQKYIDRQLFDPANSFVIGDRATDVQLAENLGIRALQYHPEKLNWDLIAEKL
                     LGEAVTNIGERPKRFAEVIRKTKETDIKVQVWLDETGNNEISTGVGFFDHMLDQIATH
                     GGFRMNITTKGDLWIDEHHTVEDTALALGTALKQAIGDKRGIARFGFVLPMDESRAEC
                     AMDLSGRPFIKFKADFKRDKVGDFSTELTEHFFQSIAFTMLATLHIKAKGDNDHHKIE
                     SLFKVFGRTLRQCIRIEGNELPSSKGVL"
     gene            2441..2725
                     /locus_tag="PXD81_002329"
     CDS             2441..2725
                     /locus_tag="PXD81_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5538309.1"
                     /translation="MIREVIDFIKNNKWYVLLVVLLLGLYFYQFHGGLSSDSEKWDHF
                     GSYIGGIFSAVTLLSVLHGMQLERKRFKEQKEEFENDKRVLEKRRRKEDF"
BASE COUNT          783 a          634 c          660 g          652 t
ORIGIN      
        1 acaatatgac aatttcacaa ctttcccgta aaaacattca agctctcacc ccttaccaat
       61 cggcacggcg tttggggggt agtggcgatg tgtggctgaa tgccaatgaa tatgcccttt
      121 cgccaaactt tgatttaacc gaccgcactt ttaaccgcta ccctgaacct caaccgcagg
      181 cggtgattga aggttacgcc cgatatgcag gcgttgcacc tgaaaacgtg ctggtttccc
      241 gtgggggcga tgagagcatt gagctgatta tccgagcctt ttgcgaggca aacgacagca
      301 ttctctactg cccaccaacc tacggaatgt acgccgtgag tgccgacacc tgtggcattg
      361 cgaccaaaac tgtgccttta accgccgatt tccagttgga tttagcccaa atcaaagcta
      421 acctcgaggg cgtgaaagtg gtgtttgtgt gcagcccgaa caacccgaca ggcaacttaa
      481 tcaagcggtc ggatttgttc gaacttttgc aaatcaccgc aggcaaagcg attgtggtgg
      541 tggacgaagc ctacatcgaa ttttgccctg aagctcgctt ggtaagcgag ctggcgaatt
      601 tcccacattt ggcgattatc cgcaccctat ccaaagcctt tgccttggcg ggtttgcgtt
      661 gtggctttac cttggcaaac gctgagctga tcggcgtgtt acaaaaagtg attgccccgt
      721 atccgctgcc tgtgcctgtt tccgacatcg ccgcccaagc cctctcccca caaggcattg
      781 agcagatgaa aacccgtgtg gcggaagtca tcaccctgcg tgccgaattg caaaaaaatc
      841 tggaaaactt accgcttgta gaacaagttt ttgacagcga agccaactac ctcctattca
      901 aatgccaaga cggacaaaaa gtcttcaaag cactttggga gcaaggcatt attttgcgtg
      961 accaacacaa agcgttagga ttgcaaaatt gcattcggat tacggtgggg gcgagagagg
     1021 agaatttgag ggtggttgag gcgattacag cactgagcca atttcagaga tagaatttaa
     1081 cgagcagtat tttaagagac atttatagcc tagcacggca tagccgtact aggttacgca
     1141 taagtcagtc cctttgggac tgaatacaga gaggcaaagt ctctcactaa tgcttaaccc
     1201 catatgcctt ggcgtgtggg gagagtaatg aataagatac agatacagga gaaaccaatg
     1261 caacccaccc tttttatcga ccgtgacggc acgttaattg atgagccaaa aaccgatttt
     1321 caaatcgaca gcttggaaaa gctgaaattc gagaaaaacg tgattcctgc cctgctcaaa
     1381 ctcaagggca aataccgctt tgtgatggtt acgaaccaag acggcttagg cacgagttcg
     1441 ttcccacagg cagatttcga caaaccgcac aatgcaatga tggcgttgtt caactcgcaa
     1501 gggattgaat ttgatgatgt gctgatttgt ccacacaagc ccgaagacgg ttgcgattgc
     1561 cgcaaaccgc acaccaaact gctacaaaaa tatatcgacc gccaactgtt cgacccagcc
     1621 aacagctttg tgattggcga ccgtgctacc gatgtgcaac tggcggaaaa ccttggcatt
     1681 cgggcgttgc aatatcaccc tgaaaaactg aactgggatt tgattgctga aaaactactg
     1741 ggcgaagccg tcaccaacat tggcgagcga ccaaagcgct ttgccgaagt tatccgtaaa
     1801 accaaagaga ccgacatcaa agtgcaagtg tggctggacg aaacaggcaa caacgaaatc
     1861 agcaccggcg tgggcttttt cgaccatatg cttgaccaaa ttgccaccca cggcggtttt
     1921 agaatgaaca tcaccaccaa aggcgacttg tggattgacg aacatcacac ggtggaagac
     1981 accgccctcg ccctcggcac cgcattaaaa caagcgattg gcgacaaacg cggcatcgcc
     2041 cgtttcggct tcgtgttgcc gatggacgaa agccgtgcgg aatgtgcgat ggacttatca
     2101 ggcagacctt tcatcaagtt caaagcggat ttcaaacgcg acaaagtggg cgatttcagc
     2161 accgagctca ccgaacactt cttccaatcc atcgccttca cgatgctcgc caccctgcac
     2221 atcaaggcaa aaggcgacaa cgaccaccac aaaatcgaaa gcctgttcaa agtcttcggc
     2281 agaacactca gacagtgcat tcgtattgaa gggaatgaac taccaagcag taaaggtgta
     2341 ttataatcac cgtaggggcg ggttttaacc cgcccgaaag tgcggttgaa atttgcaaag
     2401 tttttgggga ttttgaccgc ttgttataaa ggatatattg atgattagag aagttattga
     2461 ctttataaag aataataaat ggtatgttct gttagttgta ttgttattag gattatattt
     2521 ttatcagttt cacgggggat tatcctcaga tagtgaaaaa tgggatcatt ttggtagcta
     2581 tattggtggt atattttctg ctgttacttt attgagtgtt ttgcatggaa tgcagttgga
     2641 acgtaaaaga tttaaggagc aaaaagaaga atttgaaaat gacaaaagag tattagaaaa
     2701 gagaagaaga aaagaagatt tttagcgta
//
LOCUS       DANLYI010000236         2700 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.238, whole genome shotgun sequence.
ACCESSION   DANLYI010000236 DANLYI010000000
VERSION     DANLYI010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2700
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.238"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            170..1588
                     /gene="leuC"
                     /locus_tag="PXD81_002330"
     CDS             170..1588
                     /gene="leuC"
                     /locus_tag="PXD81_002330"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HDL5538310.1"
                     /translation="MQQAKTLYQKLFDSHVVYEAEGETPIIYINRHLIHEVTSPQAFD
                     GLRVAGRQVRQVSKTFGTMDHSISTQVRDVQQLEGQAKIQVLELEKNCEETGIPLFDI
                     TKKQQGIVHVMGPEQGLTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTL
                     KQARAKSMKVEVRGKVNPGITAKDIVLAIIGKTTMAGGTGYVVEFCGEAIRDLSMEGR
                     MTVCNMAIEFGAKAGIVAPDETTFAYLKDRPNAPKGQDWEDAIEYWKTLKADEGAVYD
                     AEVILEAKDIAPQVTWGTNPGQVIGIDDVIPNPEDMADPVTRASAEKALAYIGLEAHT
                     DMKDVPVDQVFIGSCTNARIEDLRAAAAVMKGRKKADNVKRILVVPGSGLVKEQAEKE
                     GLDKIFIEAGAEWRNPGCSMCLGMNDDRLGEWERCASTSNRNFEGRQGRNGRTHLVSP
                     AMAAAAAVFGKFMDIRKVSLNA"
     gene            1601..2203
                     /gene="leuD"
                     /locus_tag="PXD81_002331"
     CDS             1601..2203
                     /gene="leuD"
                     /locus_tag="PXD81_002331"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005595839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HDL5538311.1"
                     /translation="MAGIKQHSGLVVPLDAANVDTDAIIPKQFLQAITRVGFGKHLFH
                     EWRYLDAEETQINPDFVLNFPQYQGATILLARKNLGCGSSREHAPWALADYGFKVMIA
                     PSFADIFYNNSLNNHMLPIKLTEEEVEEIFQWVWANEGKQIHVDLEAMTVTVGEKVYT
                     FELDEFRRHCLLNGLDNIGLTLQHEAAIAAYEKNIPAFLR"
     gene            complement(2275..2700)
                     /locus_tag="PXD81_002332"
     CDS             complement(2275..2700)
                     /locus_tag="PXD81_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011200188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5538312.1"
                     /translation="MGLFACTPSPQSNSTKQQAQVGYLKQNISQAELNNPKDFKRYSY
                     YCKNFATGSSSYLATYFPLWSESRKQENFGIYFQLDGGKVYPFDHLANISLNARATKF
                     EVRYRSYQPIEGSFVELVAREFTSTYYKNNVPWLECREG"
BASE COUNT          800 a          560 c          607 g          733 t
ORIGIN      
        1 taggcttatt tagcagaata aaaaccctta tttagcataa aaatactgtt ttactttcat
       61 ttttttaatc gttatattca ctccatatca gcaagcggtc atttttgcaa aattttttgc
      121 aaatttaacc gcttgtatta aaagttaccc ttttgagttg gaacatttta tgcaacaagc
      181 caaaacccta tatcaaaaac tattcgactc acacgttgtg tatgaagccg aaggcgaaac
      241 gccgattatt tatattaacc gccacttaat tcacgaagtt accagcccgc aagcgtttga
      301 cggcttgcgt gttgccggtc gccaagttcg ccaagtcagt aaaaccttcg gcacaatgga
      361 ccacagtatt tcaacccaag tgcgtgatgt acagcaactt gaagggcaag cgaaaattca
      421 agtattagaa ttagaaaaaa actgcgaaga aaccggtatc ccactgtttg acatcactaa
      481 aaaacaacaa ggtatcgttc acgtgatggg gccggagcaa ggcttaactt tacccggtat
      541 gaccattgta tgtggtgact cgcacaccgc cactcacggg gcctttggtg cgttagcctt
      601 cggtatcggt acttccgaag tggaacacgt tttagccacc caaaccttaa aacaggcccg
      661 tgcaaaaagt atgaaagtgg aagtccgtgg caaagtaaac ccgggcatta ccgcaaaaga
      721 tattgtgctt gcgatcatcg gtaaaacaac tatggctggc ggtacaggct atgtagtgga
      781 attttgtggc gaagcaattc gtgatctttc aatggaaggg cgaatgacag tgtgtaatat
      841 ggcgattgag tttggtgcga aagccggtat tgttgccccg gatgaaacca cgttcgctta
      901 cttgaaagac cgcccaaatg caccaaaagg tcaagattgg gaagatgcca ttgaatattg
      961 gaaaaccctc aaagccgatg agggtgcagt ttacgatgcg gaagtgatct tagaagcaaa
     1021 agacattgct ccacaagtga cttggggaac caacccgggg caggtgatcg gtattgatga
     1081 tgtaattcca aatccggaag atatggccga tcctgttact cgtgcctctg cggaaaaagc
     1141 cttagcttac atcggcttag aagcccatac agatatgaaa gatgtaccgg ttgatcaagt
     1201 ctttattggt tcttgtacta atgctcgtat tgaagatttg cgtgctgcgg cagcagtaat
     1261 gaaagggcgt aaaaaagccg acaatgtaaa acgtattctc gttgtgccag gttcaggttt
     1321 ggtaaaagag caagcagaaa aagagggctt ggataaaatc ttcattgaag ccggtgcaga
     1381 atggcgtaac ccgggttgct caatgtgttt gggaatgaac gatgaccgct tgggcgaatg
     1441 ggaacgttgt gcctctacgt ctaaccgtaa ctttgagggc agacaaggac gtaacggtcg
     1501 tacccactta gtcagcccgg caatggcggc tgcggcggcg gtgttcggta aatttatgga
     1561 tattcgcaaa gtgtcattaa acgcataata ggaggaaaaa atggcaggta ttaaacaaca
     1621 ttcaggcttg gtcgttcctc ttgatgcagc gaacgtagac accgatgcaa tcattccaaa
     1681 acaatttttg caagccatta ctcgagtcgg ctttggtaag caccttttcc acgaatggcg
     1741 ttatttagat gcggaagaga ctcaaatcaa ccccgatttc gtactgaact tcccacaata
     1801 tcaaggtgca accattttac tcgcccgtaa aaatttaggc tgtggctctt ctcgtgagca
     1861 cgccccttgg gcattggcag attacggttt taaagtaatg atcgccccaa gttttgcgga
     1921 tattttctac aataacagct taaataacca tatgttgccg attaaattaa ccgaagagga
     1981 agtcgaagag atcttccaat gggtgtgggc aaatgaagga aaacagatcc acgttgattt
     2041 agaagcgatg actgtgactg ttggcgagaa agtttatacc tttgagttag acgaattccg
     2101 ccgccactgc ttattaaacg gtttggataa tatcggctta accctacaac acgaagctgc
     2161 gattgctgct tatgagaaaa acattccggc atttttacgc taattaaaat acaaaaaaac
     2221 ggctaacatc tttaaggcgt tagtcgtttt ctttttagcc attttctttg gcaattagcc
     2281 ttctcggcac tctaaccaag gcacattatt tttgtaataa gttgaagtaa actctcgtgc
     2341 cactaactct acaaaactgc cttcaatcgg ctgataagaa cgataacgca cttcaaattt
     2401 tgttgctctt gcattcaaac tgatgttggc aagatggtcg aacggataaa cctttccgcc
     2461 atcaagttga aaatagatac caaaattctc ttgcttacgg ctttcagacc aaagtggaaa
     2521 ataggttgcc aaatatgaac tgctgcctgt tgcaaaattc ttacaataat agctataacg
     2581 cttaaaatct ttcggattat tcaattccgc ttggctgata ttctgtttca aatatccaac
     2641 ctgtgcttgt tgcttagttg agtttgattg tggtgagggc gtacaagcaa ataagccaat
//
LOCUS       DANLYI010000237         2690 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.239, whole genome shotgun sequence.
ACCESSION   DANLYI010000237 DANLYI010000000
VERSION     DANLYI010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2690
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.239"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            72..830
                     /locus_tag="PXD81_002333"
     CDS             72..830
                     /locus_tag="PXD81_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDL5538313.1"
                     /translation="MIPLERQREILRLINNNGVASINFLAEQLKVSHMTIRRDIQKLE
                     EQGKVSLVSGGVQSLERLIMELPHDDKSMLFQEQKSAIGSLASQLIPSNTTIYLDAGT
                     TTLEIAHRIIDRNDLLVVTNDFVIAHYLSNSGKCDLIHIGGSVCKENLSTTGSLAAEF
                     LKNLSIDMAFISTSSWNLKGLTTPNENKIPVKKAILEASKQNILVTDSSKYGKVATFF
                     VCPLTVFDQIICDKGLFENVRDALKGLSIKLDLG"
     gene            complement(970..2361)
                     /gene="ffh"
                     /locus_tag="PXD81_002334"
     CDS             complement(970..2361)
                     /gene="ffh"
                     /locus_tag="PXD81_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011199698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HDL5538314.1"
                     /translation="MFENLSDRLSKTLRNITGKGRLTEDNIKDTLREVRMALLEADVA
                     LPVVRDFINKVKERALGVEVNKSLTPGQEFLKIVQSELETAMGEANEELNLAAQPPAV
                     ILMAGLQGAGKTTSVGKLAKFLKERHKKKVLVVSADVYRPAAIKQLQTLAEALKVDFF
                     PTETSQKPVAIAEAALKHAKLNFFDVLIVDTAGRLHVDGEMMEEIQQIHAVLNPIETL
                     FTVDAMTGQDAANTAKAFNEALPLTGVILTKVDGDARGGAALSIRQITGKPIKFLGVG
                     EKTDALEPFHPDRVASRILGMGDVMSLIEDIQRSVDQEKAEKIAAKFKKGDEFTLEDF
                     REQLIEMKKMGGMMSMLDKLPGAKNLPDHVKSQVDDKMFMKMEAIINSMTLKERANPD
                     IIKGSRRRRIALGSGTQVQDVNKLLKQFDDMQRMMKKMRKGGMAKMMRGVKGMMGGGL
                     GGLGGLGSMFGKR"
BASE COUNT          813 a          557 c          518 g          802 t
ORIGIN      
        1 aatttttgtg attttaattt tggaaactaa atctgatata atctctttta acaattaaaa
       61 taggttaaaa aatgatcccc ttagagagac aacgagaaat actacgactt attaataata
      121 atggtgtcgc aagtattaat tttctggcag aacagctaaa agtatcacat atgacaattc
      181 gccgtgatat tcaaaagctg gaagagcaag gtaaggtatc cctagtatct ggtggagttc
      241 aatctcttga acgattaatc atggaattac cacacgatga taaatcaatg ttattccaag
      301 agcagaaatc agctatagga tctttagcat cacagctaat tccctcaaac acaactattt
      361 atcttgatgc tggaactact acattagaaa tagctcatcg gataatcgat agaaatgact
      421 tactagttgt aacaaacgat tttgttattg ctcactatct tagtaattca ggaaaatgtg
      481 atttaattca tataggagga agtgtctgta aagaaaacct atcgacaact ggttcacttg
      541 cagcagaatt tctgaaaaac ctatcaatcg atatggcatt tatatctact tcatcctgga
      601 atttaaaagg tctaacaacg ccgaacgaaa ataaaatccc cgttaaaaaa gcaattttag
      661 aggcatccaa gcaaaacatt ttagtgacag actcttcaaa atatggaaaa gtcgcaactt
      721 tttttgtttg ccctcttact gtatttgacc agattatttg tgataaagga ttatttgaga
      781 atgtacgaga cgcactgaaa gggctaagta ttaaattaga tttaggataa tcttcttacc
      841 aattttttct gagaacgaca agcaatcaaa aagtattatt tttgcaaata agatattaat
      901 agggaaagtt aacaatccta gcaacggatt gttaactgat tttcggctgg tactaataaa
      961 ataggaatat taacgtttac caaacatact gcctaagcca ccgagaccgc ctagaccacc
     1021 acccatcata cctttaacac cacgcatcat ttttgccatt ccgcctttac gcattttttt
     1081 catcatacgt tgcatatcat caaactgctt gagcagctta tttacatctt gcacctgtgt
     1141 gccggagcca agtgcgatac ggcggcggcg tgagccttta ataatatccg gatttgcccg
     1201 ctcttttaag gtcatagaat tgatgatggc ttccattttc atgaacattt tatcatcaac
     1261 ctgactttta acgtggtcag gcaggttttt cgcccccggt agtttatcga gcatcgacat
     1321 cattccaccc atttttttca tttcgataag ctgctcacgg aaatcttcca aggtaaattc
     1381 atcgcctttt ttgaatttgg cggcaatttt ttccgctttt tcttgatcaa ccgagcgttg
     1441 aatatcttca attaaagaca ttacatcgcc catacctaaa atgcgggaag ccacacggtc
     1501 agggtggaac ggctcaaggg catcagtttt ttcacccaca cctaagaatt taatcggttt
     1561 accggtgatt tggcgaatag aaagtgccgc accgcctcgg gcatcgccat cgactttggt
     1621 aagaataaca ccggtaagcg gcaacgcttc gttaaaggct tttgcggtgt tggcggcgtc
     1681 ttgaccggtc atcgcatcaa ccgtaaatag cgtttcaatc ggattcagca cagcgtggat
     1741 ttgttggatc tcttccatca tttcgccatc aacgtgtaaa cgaccggcag tatccacaat
     1801 caatacatca aagaaattaa gcttagcgtg cttcagtgcc gcttctgcaa tcgctaccgg
     1861 tttttggctg gtttcggtcg ggaagaagtc cacttttaac gcttcagcaa gggtctgtag
     1921 ctgcttgata gcggcagggc ggtaaacatc ggcagaaact accaacacct ttttcttgtg
     1981 gcgttctttc aggaattttg ccaatttgcc gacagaggtg gttttacccg caccttgcaa
     2041 acctgccatt aaaattaccg caggcggttg ggccgcaaga ttcagctcct cgttggcttc
     2101 tcccattgcc gtttctaatt cgctttgtac gatttttaag aactcttgcc ccggcgtgag
     2161 gcttttgttt acctcaaccc ctaacgcacg ctctttgacc ttgttgatga aatcacgcac
     2221 aacagggagg gcaacatccg cctccaacaa tgccatacga acttcacgca gggtatcttt
     2281 aatattatct tccgttaggc gacctttgcc cgtaatgtta cgcagcgttt tagacagtct
     2341 gtcagagagg ttttcaaaca tatcagcttt ctcaatttgc aaaattttag gaaaaaagta
     2401 ccgcttgtga gcggaaagtt gccgccatta tacaaaaaaa agcgttacgg ttatagggaa
     2461 ataaaaaaac ggcatagcga ttaaactatg ccgtaaaatt tgacgatatt cgaggaatca
     2521 ttatttagtt atattgtgcg atcataatac tccgaattgc gtataaaagt gaagtgatat
     2581 tttcaaattt atgttagaaa tgagaggtaa attacaaaat taaaggggat attattgttt
     2641 gaaaaaataa gagcgaaaag aaaaccctga tgattgctca tcagggcttc
//
LOCUS       DANLYI010000238         2672 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.240, whole genome shotgun sequence.
ACCESSION   DANLYI010000238 DANLYI010000000
VERSION     DANLYI010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2672
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.240"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            71..565
                     /locus_tag="PXD81_002335"
     CDS             71..565
                     /locus_tag="PXD81_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HDL5538315.1"
                     /translation="MTEYLTSFFSLFFSEQNQLFTMFLSGFLSATLLPGNSEIIFTTL
                     ASQHLITYGSLSSFSLFNLLLVATLGNSLGSITTYAIGLLLPRPQNTTNRYSQWALAK
                     SEKYGVLVLLFSWLPIVGDIFCGIAGWLRFSLWQSILFIVIGKLLRYLFLLATLYSVL
                     PHIL"
     gene            complement(619..1488)
                     /gene="htpX"
                     /locus_tag="PXD81_002336"
     CDS             complement(619..1488)
                     /gene="htpX"
                     /locus_tag="PXD81_002336"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005604779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HDL5538316.1"
                     /translation="MAKRVILFLLTNLAITFVLGIVLNLIFKVTGIQGQSTAGILVLS
                     LVFGFSGSLISLFMSKSMALRSVGAEVITQPRNEAEQWLFNTVQRQAQQANIPMPDIA
                     IYHSADVNAFATGATKSNSLVAVSTGLLNSMTKDEAEAVVAHEVAHIANGDMVTMTLL
                     QGVLNTFVIFLARIISTMAASSRDENGNSSTNTLVFWVVDIVLQMVFGVLATMIAMWF
                     SRQREFRADAGSAQLVGKEKMIAALQRLQRVHEPQEMEGSLAAFMINGSRGKELFMSH
                     PPLEKRIEALRNL"
     gene            complement(1576..2658)
                     /gene="dinB"
                     /locus_tag="PXD81_002337"
     CDS             complement(1576..2658)
                     /gene="dinB"
                     /locus_tag="PXD81_002337"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HDL5538317.1"
                     /translation="MSIQAKHKRKIIHIDMDCFYAAVEMRENPALVGKPVAVGGNANQ
                     RGVLTTCNYEARKFGLHSAMPTATALKKCPNLILLPVNMALYKSVSAQIHEIFHRYTD
                     VIEALSLDEAYLDVTECERYFGSATWIAQAIRNDIFNELHLTASAGVAPLKFLAKITS
                     DQNKPNGQCVITPDDVQNFVKNLPLAKIPGVGKVTNEKLKQLGFETCGDIQQAEKAVI
                     YRAFGKFGQRLWDFSHGIDERAVEPYRERKSLAVENTLLHDISTLEQAEKIVADLFDK
                     LVFRLQRNWGDTPLTEFKKLGIKLKFDDFSQTTLERTTDGFAYERFGELLQQIFTRRK
                     ERKVRLIGLSVHFPEKKVNKQLNLWE"
BASE COUNT          766 a          614 c          473 g          819 t
ORIGIN      
        1 aaatccataa aaaacacaac attcaagaaa atttataact acattttaat aacaaaccaa
       61 ctacaatttt atgacagaat atctcacttc ctttttcagt ttattctttt ccgaacaaaa
      121 tcaactcttt actatgtttc tgagtggatt tctaagcgct accctcttac ccggaaattc
      181 tgaaattatc ttcactacct tagcaagcca acatctcatc acttatggct ctttatcctc
      241 attttcactg ttcaatttac tcttggttgc aaccttaggt aatagtttag gtagcataac
      301 cacttatgcg atagggctat tactccctcg cccacagaat actaccaatc gttattcaca
      361 atgggcatta gccaaaagtg aaaaatatgg cgtgttagtc ttgcttttta gttggttacc
      421 gattgtaggc gatattttct gtggcattgc cggctggcta cggtttagcc tttggcaaag
      481 cattctcttt attgtgattg ggaaattgtt acgttatctg tttttgctgg caactctcta
      541 ttcggtgtta ccgcatattt tatagaaaac aaaagcggct gttttgcaaa aaattttaca
      601 aaaacaaccg cttgtgcttt ataagtttct taacgcttca atacgttttt ctaacggagg
      661 gtggctcata aacaactctt taccacggct accgttaatc ataaaggcag caagagagcc
      721 ttccatttct tgcggttcgt gaacacgttg caagcgttgt aatgccgcaa tcattttctc
      781 tttaccgact aattgagcag agcctgcatc tgcacgaaat tcacgttggc gagagaacca
      841 cattgcaatc attgttgcta acacaccaaa caccatctgt aacacaatat ctactaccca
      901 gaacaccagc gtattggtgc ttgaattgcc attttcgtca cgtgaacttg ccgccattgt
      961 cgaaataata cgagctaaga aaatgacgaa agtatttaac acgccttgta ataaagtcat
     1021 tgtaaccata tcgccattcg caatgtgcgc cacttcgtgt gctactaccg cttcagcttc
     1081 gtctttagtc attgaattca gcaagccggt gctgactgca actaaagaat tacttttcgt
     1141 tgctcctgta gcgaatgcat ttacatcagc cgaatggtaa atcgcaatgt ccggcattgg
     1201 gatattcgct tgctgagcct gacgttgtac agtgttgaat aaccattgct ccgcttcgtt
     1261 acgaggctgt gtaatcacct ctgccccaac cgaacgcaat gccattgatt tcgacataaa
     1321 tagcgaaatt aaagacccac tgaaaccgaa gactaacgaa agcaccaaaa taccggcagt
     1381 gctttgccct tgaatgccgg tcactttaaa aataaggttt aatacgatcc ctaaaacaaa
     1441 ggtaatcgct aagttagtta ataaaaatag aatgacacgt tttgccataa tgttttcaat
     1501 ctccaaaaat atagatgcaa tgattgcacg aataagataa tgactttagc cttaatttca
     1561 agtcattatt tacaattatt cccacaaatt taactgttta ttcacttttt tctcgggaaa
     1621 gtgaacactt agaccgatta atcgcacctt tcgttccttt cttcgggtaa aaatttgctg
     1681 tagcaactcg ccaaatcgct catacgcaaa gccatcggtg gttcgttcaa gcgtggtttg
     1741 gctaaaatca tcaaacttga gtttaatgcc taacttttta aactcagtta atggcgtatc
     1801 gccccagttt cgttgcaacc gaaaaaccag tttgtcgaat aaatctgcca caatcttttc
     1861 cgcttgctca agggttgaaa tatcgtgcaa cagcgtattt tccaccgcca gcgattttct
     1921 ttcacgataa ggctctaccg ctcgttcatc aataccgtgg ctaaaatccc aaagccgttg
     1981 cccgaatttg ccaaacgctc ggtaaataac cgctttttct gcttgctgaa tatcaccaca
     2041 ggtttcaaag ccaagctgtt ttaatttttc attggttact ttccccacac ccggaatttt
     2101 tgcaagcggt agatttttca caaaattttg cacatcatca ggcgtaatca cacattgtcc
     2161 gttaggtttg ttttgatcgg aggtaatttt tgctaaaaat ttaagcggag caactcctgc
     2221 cgaggcagtt aaatgcaatt cattgaaaat atcattgcgg attgcttgag caatccaagt
     2281 ggcggagcca aaatagcgtt cgcattcggt tacatctaaa taggcttcat caagcgacaa
     2341 ggcttcaatc acatccgtat agcgatgaaa aatttcgtga atttgagcgg aaaccgattt
     2401 atacaacgcc atattcaccg gcaataaaat taaattcggg catttcttca acgcagttgc
     2461 cgttggcatt gcactgtgta agccgaattt tcgagcctcg taattacagg ttgtcagcac
     2521 acctcgttga ttagcattgc cgcccaccgc caccggctta ccgaccaacg ccgggttttc
     2581 ccgcatttcg accgctgcat aaaaacaatc catatcaata tggataattt ttcgcttatg
     2641 tttagcttga atcgacattt gcaatatttt cc
//
LOCUS       DANLYI010000239         2614 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.241, whole genome shotgun sequence.
ACCESSION   DANLYI010000239 DANLYI010000000
VERSION     DANLYI010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2614
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.241"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            <1..>2614
                     /locus_tag="PXD81_002338"
     CDS             <1..>2614
                     /locus_tag="PXD81_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HDL5538318.1"
                     /translation="DFPAIQKSFLGTKLFFKDDSTEVVLSKLAKKQTDSLLLEIEKVV
                     ASNIKVKVKEGFQHFANLAENQYLRDSDIPTLNDRVRLSVLSYGDNKEHFQKYFDESL
                     VKKIQYISSLFPVNAINAQTIRREYEQHVLNHRKHFFDVIESNPLTEQQRLSVIRNND
                     INMVLAAAGTGKTSVMVAKALDLIDSGACKSEDILILAYNRDAATELSERLQECAREA
                     NLDLENSPSISTFHALGRRIIKECGKSVHLSKFAEDPMKLDRWFNKWLEDYIKEDPDN
                     LAKFIQLAYQPIDPFQFKTKDEYDRYVNDNDYRTLQGERVRGYQELLIANWLFSHSVE
                     YEYEPRYVSKRRIEIGFDYKPDFSLGDGVYLEHFGIDRQGRTRADINAQEYNANIQRK
                     RELHAEHNTTLLETYHYNWVENTLYKRLEQLMNEQFIPLKPKSQQEILDALNESGIFK
                     ENKNRYLKCLQAIRTERLDYQQILKRLTDAKIVYAKEYATLLMRIHDAYVKELRSANE
                     IDFDDMILLATQLVKTGEFKPKWKHILVDEFQDISMARLELLKEIYTKGPRPIWTVVG
                     DDWQSIYRFSGGKLEVTTRFNDMVGSHTLSKLEKTYRYNNSIADTAGQFIMQNPEQYQ
                     KNVVTHTKVADSCVHLYDSHVVKDEKSEANISLKASAILKLIRQKAPEATVAILARYR
                     YLLEDAKSVIKDKNVKFWTFHGSKGLEADYCILLGFFQGKVGFPNQNKEEAVVEALLP
                     MLDSFPHSEERRLFYVALTRAKKESYLIADANAPSEFINELLSPKYKIDIVSKRFQER
                     YRTIFKCNECSTGYFVMKQGKFGQFYSCTSGEVCRSSPRICEKCGRPMKLREGKFGQF
                     WGCSGYGIKGDQCTN"
BASE COUNT          898 a          474 c          513 g          729 t
ORIGIN      
        1 tgactttcca gctatccaga agtctttctt aggtactaaa ttatttttta aagacgatag
       61 cacagaagtt gttttatcaa aacttgcgaa aaaacaaact gatagtctat tacttgaaat
      121 cgaaaaagta gttgcatcta atattaaagt gaaagtaaaa gagggttttc aacactttgc
      181 aaatttagcc gaaaatcaat atctcagaga ttccgacatt cctacattaa atgatcgtgt
      241 aagattgtca gtgctttcct atggggataa caaagaacat tttcaaaaat actttgatga
      301 aagtttggtt aagaaaattc aatatatctc ttcacttttt cctgtaaatg ccataaatgc
      361 tcaaacaatt cgaagagaat acgagcaaca tgttttaaat catcgcaagc atttctttga
      421 tgtcattgaa tctaatccgc ttactgagca acaaagattg tcagtcattc ggaacaacga
      481 catcaatatg gttcttgctg ccgcggggac aggaaaaacg tctgtaatgg tcgcaaaagc
      541 attggactta attgattctg gtgcttgtaa aagcgaagat attttgattc ttgcatacaa
      601 ccgcgatgca gcaaccgaac tatccgaacg cttacaagaa tgtgcacgag aagccaattt
      661 agatctggaa aatagcccta gcatctcgac tttccatgcg cttggccgaa gaattattaa
      721 agaatgtgga aaatcagtcc acttgtcaaa atttgccgaa gatccgatga aattagatag
      781 atggttcaac aaatggctag aggactacat caaagaagat cctgataatc ttgcaaaatt
      841 cattcaactt gcataccaac ctatcgatcc attccaattc aaaacgaaag acgaatacga
      901 tagatatgta aatgacaatg actatcgaac cttacaaggc gaacgcgttc gtggttatca
      961 agagttattg atcgcgaact ggctttttag ccacagcgta gaatatgagt atgaaccaag
     1021 gtatgtatca aaacgccgta tcgagattgg ttttgactac aaacccgatt tctctttggg
     1081 tgatggtgtt taccttgaac atttcggcat tgaccgacaa ggtagaactc gcgcagatat
     1141 taacgcgcag gaatataatg ccaatatcca aagaaaacgt gagttacacg ccgaacataa
     1201 tacaacatta ctggagacat accactacaa ctgggtggaa aatacgttat ataaacgact
     1261 agagcaattg atgaatgaac agttcattcc tctgaagcca aaatcacaac aagagatttt
     1321 agatgcactc aatgaatcag gaatcttcaa agaaaacaaa aatcgctatt tgaagtgcct
     1381 tcaagctatt cgtacagagc gattagacta tcaacaaatc ttaaaacgtc taaccgatgc
     1441 gaaaattgtc tatgctaaag aatatgcgac attgctcatg cgtattcatg atgcctacgt
     1501 taaagaacta cgtagcgcaa atgaaatcga ctttgacgat atgattttgt tggctactca
     1561 actggtgaaa acaggtgagt ttaaacctaa atggaaacat attttagtcg atgaatttca
     1621 agatatttct atggctcgat tggagttatt gaaagaaatt tatactaaag ggcctcgacc
     1681 gatttggact gttgttggag atgactggca atcaatctat cgtttctctg gcggtaaact
     1741 agaagttact acgcgattta atgatatggt cggctcacac acgctaagta aactagaaaa
     1801 aacctaccgc tataacaata gtattgctga cacagcaggt caattcatta tgcaaaatcc
     1861 tgagcagtat cagaaaaatg ttgtaacgca tacaaaggtc gctgattctt gtgttcattt
     1921 gtatgactct catgtcgtaa aggatgaaaa atcagaagcc aatatcagtt taaaagcctc
     1981 agcaatatta aaactgatac gtcaaaaagc acctgaagcc actgtcgcaa ttttggctag
     2041 ataccgttac cttttagaag atgcaaaaag tgtaatcaaa gataagaatg ttaaattttg
     2101 gacgttccac ggctcaaaag ggttggaagc agattactgc attttacttg gtttcttcca
     2161 aggaaaagtc ggatttccca accaaaataa agaagaagcc gttgtagaag cattgcttcc
     2221 tatgctagac agttttccac actcagaaga acgtaggtta ttttatgtgg cattaacgcg
     2281 tgcgaaaaaa gaaagttatt tgatcgcaga tgcgaacgcg ccatcagaat ttatcaatga
     2341 attattatca ccgaaataca agatagatat tgtgtcaaaa cgattccaag aacgttatcg
     2401 gacaatcttt aaatgtaatg aatgtagcac tggctatttc gtgatgaaac aaggtaaatt
     2461 tggtcagttc tattcttgta catcagggga agtatgccgc tcttcgccac ggatttgtga
     2521 aaaatgtggc cgtcctatga agctgcgaga aggtaaattt ggtcagtttt ggggatgctc
     2581 aggatatgga atcaaaggcg atcaatgtac gaat
//
LOCUS       DANLYI010000240         2602 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.242, whole genome shotgun sequence.
ACCESSION   DANLYI010000240 DANLYI010000000
VERSION     DANLYI010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2602
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.242"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            104..391
                     /locus_tag="PXD81_002339"
     CDS             104..391
                     /locus_tag="PXD81_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDL5538319.1"
                     /translation="MAKTLRISSRAQNDIEEILASVIEYTGFESSGIRLQEDIYQKFE
                     TIAYMPSAAGRLREDGTREAFTRRYRIVYTELENEVWIITIIHSSRLYPRI"
     gene            complement(517..2580)
                     /locus_tag="PXD81_002340"
     CDS             complement(517..2580)
                     /locus_tag="PXD81_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012263170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HDL5538320.1"
                     /translation="MSVLAANAVAQTASTDAVVLDTVTVTDNQGLKVQSNVVTTKKKD
                     ESTQTDLRGLLKDEPAISLGGGNGTSQYLYIRGMGQNSIDVKVDNTYSDSQILYHQGR
                     HMLDPALVKIVSVQKGAGSASAGIGQTNGAVIAKTVDALDLLKNSDKNFGAKLGTGFS
                     TNHAHNFNATLYGKGEIFDALVSGNLVRDRDYKGGKDYVNQFGTNRVPYSALDKTSFL
                     AKLGATLGDHRFVLSHSSERNEGQRLVREEFDVAPNGTKVSPYNRLTVERQAPANRRI
                     TVQRTNLEWTGKNLGFAQEATANVYQLVQGRWSENETGNGYAGGVDYATKTKIVTHGA
                     NVNFDSALHENVLLKYGVNYRHQEAKPHTKFNPLVKNQEKTDTGVYVEAITAPVDKVT
                     LTTGVRYDHFKFKAMDGKERSNGAFSPSVGLIYEPIEHLSLSASHNYATRSPRLYDAL
                     TAHGNRKIVSIGSNTKAEKARNTEIGFNYNNGTFSFDGSYFWQDIKDALGGGRDSHNP
                     NNELIMNLGKIKNRGYELNAGYHNGGFTARIGVAHSKPRFYSPTIWEVNRQTGKREEK
                     PLLSTNPEYASAIGRTWTASLSYRFEQPNLEVGVHHRIVEKVKPEDNFFVTGGTLQTN
                     TGTGKDGYNVTDITLNWKPLKDDSVNVNFAVDNVANKKYNAHGQRGQLSARGREFRAG
                     VNYTF"
BASE COUNT          742 a          527 c          490 g          843 t
ORIGIN      
        1 tcaggtaatg gtctttcact cgatgaatca aaagcacgaa ccaaacaact gattgaacgt
       61 aaagcaagag aactcgcaaa ctttgaacaa gagaatatca tttatggcta aaacattacg
      121 aattagcagt agagctcaaa atgatattga agaaatttta gcgagcgtaa ttgaatatac
      181 tggttttgaa agtagtggaa ttcgcttaca agaagatatt taccaaaaat ttgagactat
      241 cgcttatatg ccctctgcgg ctggacgttt aagagaagat ggcacaagag aagcattcac
      301 taggcgttat cgtattgttt ataccgaatt agaaaatgaa gtgtggatta ttaccattat
      361 tcatagtagc agactttatc cacgcattta aataacaaac gactaaaata gtcggttttt
      421 tatttgcaaa aaattaataa aatatcaccg cttgtaagct ccataaaaaa aagcccgatt
      481 gtaacatcgg gcgttttagt ctgaatttaa gtcagattag aatgtgtagt tcacacctgc
      541 acggaattca cgtccacgag ctgataattg accacgctga ccgtgtgcgt tgtatttttt
      601 attcgccacg ttatccactg cgaagtttac attcacgcta tcatctttaa gtggtttcca
      661 gtttaaggtg atatcagtta cattgtagcc gtctttgcct gtaccggtat ttgtttgtaa
      721 tgttccgccc gtaacgaaga aattatcttc aggtttcact ttctcaacaa tacggtggtg
      781 tacgccaacc tctaaatttg gttgttcaaa gcgatagctt aaagaagctg tccaagtacg
      841 accgatagct gaggcgtatt ctgggttagt acttaataat ggtttttctt cccgcttacc
      901 agtttgtctg tttacttccc aaatagttgg tgagtagaaa cgcggtttgc tgtgtgcaac
      961 accaatacga gcagtaaaac caccattatg gtaacctgca tttaactcat aaccacggtt
     1021 tttaatttta cctaggttca taattaactc attattagga ttatggctat cacgaccacc
     1081 acctaatgca tcttttatat cttgccagaa atagctacca tcaaaactga atgtaccatt
     1141 gttatagttg aaaccgattt cagtattacg agctttctcc gctttagtat tgctaccaat
     1201 actaacgatt tttctatttc cgtgagccgt tagtgcatcg tataaacgag ggctacgagt
     1261 tgcatagttg tggcttgcac ttaagcttaa atgttcaatt ggctcataga taagcccaac
     1321 gcttgggctg aatgcaccat tactacgctc tttaccatcc attgctttga atttgaagtg
     1381 gtcgtaacgt acaccggtag ttaaagttac tttatctact ggagcagtaa ttgcttcaac
     1441 atacacgccg gtatcggttt tctcttggtt tttcacaaga gggttaaatt tagtgtgtgg
     1501 tttagcttct tggtggcgat agttcacacc gtattttaat aatacatttt cgtgaagggc
     1561 agaatcaaag tttacgtttg caccgtgggt tactatttta gttttggttg cataatctac
     1621 accaccagca taaccattac ctgtttcatt ctcagaccaa cgaccttgta ctaactgata
     1681 cacgttagct gttgcttctt gagcaaaacc taagttttta ccagtccatt ctaagttagt
     1741 tctttgaacc gtaattctac ggttagctgg agcttggcgt tctacagtta agcggttgta
     1801 cggagaaacc tttgtgccgt taggtgctac atcaaattct tcacgcacta aacgctgacc
     1861 ttcatttcgc tcacttgaat ggcttaatac aaaacggtga tctcccaatg ttgcacctaa
     1921 tttagctaaa aagctggttt tatctagtgc actatacgga acacggtttg tgccgaattg
     1981 gtttacgtaa tctttaccgc ctttatagtc gcgatctctc acaagattac ctgagactaa
     2041 agcatcaaag atttcgcctt taccatataa ggttgcattg aaattgtgag cgtggtttgt
     2101 gctgaagcca gtacctaatt ttgcaccgaa gtttttatcg ctgtttttta ataaatctaa
     2161 tgcatcaacg gttttagcaa ttactgcacc attagtttga ccaataccgg ctgaagcact
     2221 gcctgcacct ttttgtacag aaacaatttt caccaatgcc ggatctaaca tatgacgacc
     2281 ttggtgatac aaaatttggc tatctgagta agtgttatct acttttacat caattgagtt
     2341 ttgacccata ccacgaatat agagatattg tgatgtaccg ttaccaccac ctaagctaat
     2401 tgccggctca tcttttaata aaccacgcaa atcggtttgg gtgctttcat cttttttctt
     2461 agttgttacc acattgcttt gtacttttaa accttgatta tctgttacgg taacagtatc
     2521 taatactact gcatctgttg aagcagtttg agccactgca ttagcagcaa gtacgctcaa
     2581 taccgcagca gaaacaggga gc
//
LOCUS       DANLYI010000241         2530 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.243, whole genome shotgun sequence.
ACCESSION   DANLYI010000241 DANLYI010000000
VERSION     DANLYI010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2530
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.243"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            176..1291
                     /gene="wecB"
                     /locus_tag="PXD81_002341"
     CDS             176..1291
                     /gene="wecB"
                     /locus_tag="PXD81_002341"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015703970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HDL5538321.1"
                     /translation="MRYLIVFGTRPEAIKMAPLVEGFKKSGLNFKVCVTAQHRQMLDQ
                     VLELFEITPDYDLDIMSNKQTLSTVTSAILEKIQPVIAEYKPTTIFVHGDTATTLASS
                     LAAYYNQIDIAHIEAGLRTHNIYSPWPEEGNRKLTAALAKYHFAPTQSTKKNLLNENI
                     AEGTIYVTGNTVIDALFLACKKLDENIDLLSSLQEKFSYIKSKRTILITGHRRENFGN
                     GFENICKAVSTLASDFPDVQFIYPVHLNPNVREPVNRLLADKSNVHLIEPCDYLSFVY
                     LMRESYLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVEAGTVKLVGTAQSEIIKQV
                     TELLNDQAAYTKMSEAHNPYGDGTAVKQILSVFQSKQ"
     gene            1306..>2530
                     /gene="wecC"
                     /locus_tag="PXD81_002342"
     CDS             1306..>2530
                     /gene="wecC"
                     /locus_tag="PXD81_002342"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012263280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HDL5538322.1"
                     /translation="MSNFNTISVVGLGYIGLPTATVFAQHGLNVIGVDVNQHAVDHIN
                     QGKIHIVEPDLDVAVHQCVSSGKLKATLTPEPAEAFLIAVPTPFKGEDYEPDLSYIQA
                     ACKAIAPVLEKDNLVILESTSPVGATEQMAEWLAEYRPELSFPQQAGEDSDIRIAHCP
                     ERVLPGQVMRELIENDRIVGGMTNKCSKQAVDLYKTFVKGDCIVTNARTAEMCKLTEN
                     SFRDVNIAFANELSIICDRLDINVWELIALANRHPRVNILQPGCGVGGHCIAVDPWFI
                     VNKTPDLAKIIRTAREVNDYKPEWVISKVNEAVIEALQKTGKSIDQIKIACLGLAFKP
                     DIDDLRESPALKITEKLAEKYVGQILAVEPNVEELPKKLAKKNISLVTVDEALKLADV
                     VVVLVDHREFRELAAI"
BASE COUNT          823 a          489 c          479 g          739 t
ORIGIN      
        1 tttctaacct accggatact tacaagaatt tacataaaat atctacaaag taaaagtttt
       61 tatacaccta actccataaa aacattataa tgaacacgat ctttataact aatttaacaa
      121 aattacttac atctagtgta ttttagtttt atctatattt ttaaaggagt aataaatgcg
      181 ctatctcatc gtcttcggta ctcgtcctga ggcaatcaaa atggctccct tagttgaggg
      241 ctttaagaaa agcggtctaa attttaaagt ctgtgttact gctcagcacc gccaaatgct
      301 ggaccaagtt ttagaattat tcgaaattac tccagattat gacctggata ttatgagcaa
      361 taagcaaaca ctttctactg taacttctgc tattttagaa aaaattcaac ctgttattgc
      421 cgaatacaaa ccaactacta tttttgttca tggcgataca gcaaccactc ttgcctcttc
      481 attagcagcc tattacaacc aaattgatat cgctcatatt gaggcaggtc taagaacgca
      541 taatatctac tctccttggc cggaagaagg caatcgcaaa ttaacagctg ctttggcaaa
      601 ataccatttc gcaccaacac aaagcaccaa gaaaaatcta ctcaacgaaa atattgctga
      661 gggcaccatt tatgtaacgg gtaatacggt aattgatgcc ttattcctcg cttgcaaaaa
      721 attagatgaa aatattgatt tattaagctc gctacaagag aaattttctt atataaaaag
      781 taagcgtact attttgatca ccggacatcg ccgcgaaaat ttcggtaatg gttttgagaa
      841 catctgtaaa gccgtttcaa cattagcgtc tgatttccct gacgttcaat ttatctaccc
      901 cgtacacctc aacccaaatg taagagaacc ggtcaatcgc ctattagcgg ataaatccaa
      961 tgtgcatctc attgagcctt gcgactatct ctcctttgtt tatttaatga gagagtctta
     1021 ccttatctta actgactccg gaggtattca agaagaagcc ccatcattag gaaaaccggt
     1081 attagtaatg agagacacaa cagaaagacc ggaagctgta gaagccggaa cagtaaaact
     1141 tgttggaact gcacaatctg aaattattaa acaagttact gagttactca atgatcaagc
     1201 tgcttataca aaaatgtctg aggctcacaa cccttatggc gatggcacag ctgtgaaaca
     1261 aattctctca gtatttcaat caaaacaata ataagggata aatgtatgtc gaactttaat
     1321 accatttcag tagtaggctt aggctatatt ggcttaccaa ccgccactgt atttgcccaa
     1381 cacggtctaa atgtaattgg cgtagatgtt aatcagcacg ccgtagacca tatcaatcaa
     1441 gggaaaattc acattgtcga gcctgattta gatgttgctg ttcaccaatg tgtatctagc
     1501 ggaaaattaa aagccacact gacacctgaa ccagcagaag catttttaat tgctgtacca
     1561 acgcctttta aaggcgagga ttatgagcct gatttaagtt atattcaagc tgcttgtaaa
     1621 gctattgcac ctgtgctgga aaaagataat ttagtcattt tagaatccac ctctccggtt
     1681 ggcgcaacag aacagatggc tgagtggctt gccgagtacc gtccagagct aagcttccct
     1741 cagcaagcag gtgaagattc tgatattcgt atcgcccatt gcccagaacg agtgctacca
     1801 gggcaagtga tgcgtgaatt gattgaaaat gaccgtattg tgggtggtat gacaaataaa
     1861 tgctctaaac aagcggtcga tttatacaaa acttttgtaa aaggcgattg tatagttacc
     1921 aatgctcgta ccgcagaaat gtgcaaattg accgaaaata gtttccgtga tgtcaatatt
     1981 gcttttgcaa atgagctttc tattatttgt gatcgcctgg atattaatgt atgggaacta
     2041 attgctttgg ctaacagaca cccacgagtc aatatcttac aaccgggttg tggtgtgggt
     2101 ggacactgca ttgctgtaga tccttggttt attgtgaata aaactcctga cttggcaaaa
     2161 attattcgta ctgctcgtga agtaaatgac tataaacctg aatgggttat ttcaaaagta
     2221 aatgaagcag taattgaagc tttgcaaaaa acaggaaaat ctatcgacca aatcaaaatt
     2281 gcttgcctag gtttggcttt taaacctgat attgatgatt tacgtgaaag cccggcctta
     2341 aaaattaccg aaaaattggc agaaaaatat gtaggtcaaa tcttagcagt tgagccaaat
     2401 gttgaagagc taccgaaaaa attggctaag aaaaatatct ctttagttac tgttgatgaa
     2461 gcactaaaac tggctgatgt agtggttgtg ttggtggatc atagagaatt tagagaactt
     2521 gctgcgattc
//
LOCUS       DANLYI010000242         2509 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.244, whole genome shotgun sequence.
ACCESSION   DANLYI010000242 DANLYI010000000
VERSION     DANLYI010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2509
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.244"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            complement(<1..204)
                     /locus_tag="PXD81_002343"
     CDS             complement(<1..204)
                     /locus_tag="PXD81_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDL5538323.1"
                     /translation="MRIIKRRYPKLNANEITTDMLIEWRNQIVGDKIKAVTWNNYVRH
                     LKSLYNFGLSQGVLNINQNPFNQL"
     gene            complement(303..2282)
                     /locus_tag="PXD81_002344"
     CDS             complement(303..2282)
                     /locus_tag="PXD81_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraI domain-containing protein"
                     /protein_id="HDL5538324.1"
                     /translation="MQSIFKKIFGKHTSQSAVKSDTVFSIEQDDDGWLYPQNATQLLN
                     TELRQKYLGLLWQQVSMNRQMFETFYQQPIERYAEMVQLLPASESHHHSHIGGMLDHG
                     LEVIAFSAKARQSYILPPNVAPEEQSKQRDAWTAAAIYLALVHDIGKVVVDIEITLKD
                     GSRWFAWNGEPPEPYKFKYIKGRDYHLHLVIGSFFANQIIPKSAFDWLAQYPEVISSL
                     MYAMSGHNDKAGLLSEIVQKADQYSVTLALGGDVNKLVQRPVNSLSKQLVLALRYLLE
                     QKIKLNTPKGPADGWFTEEGLWLVSKTAADNIRTYLLGQGISVPKDNPKLFDEMQSLG
                     IVEESPSGGAIWSCRIQADSGWTPANVFTLLRIKPEVAWENINDRPNYFSGKVKIVEQ
                     IMEDSNMENHPPLVDESTKLVTLENVQPETIITSKEVEISQVEASSDNAPFSEIIIND
                     ENDLTSFVLDMFAPTESSEPSLADISIGKGQEIATMPSKQEVQDKPTTKKLNVTQSAV
                     ETPETLTLSELQSQNKENSITGDNFVNWLKSGIATNKFAINKPTAKLHIVEDHLFLVT
                     PVIFEMYLQESGLPYDKEMINSLQYDFQGLGIHLPKNTTINGKPDTINFWKCDVIGPR
                     KTSSLTGYLIKNTHLFFGDKTLLNNHCLTLQKEET"
BASE COUNT          722 a          485 c          473 g          829 t
ORIGIN      
        1 taactgatta aatggatttt gatttatatt gagtacccct tgcgatagac caaagttata
       61 taacgatttt aaatgtctga cataattatt ccatgttaca gctttgattt tatcgccaac
      121 aatttgatta cgccattcaa ttagcatatc agtggtaatc tcatttgcat ttaactttgg
      181 gtaacgtctt ttgataattc ggattacatt attatagctc cgcttagtat cagggcgaag
      241 atagtaacga tagaaaaaaa aatcggtcta gtagcgaatc aaaagagata gatgtcgtca
      301 ttttaagttt cctctttctg taaagttaaa cagtgattat ttaatagtgt cttatcccca
      361 aagaagagat gtgtattttt tatcaaatat ccagttaaag aggaagtctt tctcggacca
      421 attacatcac atttccaaaa attgatggta tctggcttac cgtttattgt cgtattctta
      481 ggtaaatgga ttcctagccc ttggaaatca tactgaagac tatttatcat ttccttatca
      541 taaggcagac cactctcctg caaatacatt tcaaaaataa cgggggtaac taaaaacagg
      601 tggtcttcaa caatatggag cttggcagta ggcttgttaa ttgcaaattt gttggtcgca
      661 atacctgatt tgagccaatt tacaaagtta tctcctgtaa ttgagttctc cttattctgt
      721 gactgtagct ctgatagtgt taatgtttcg ggcgtttcta ctgcactttg tgttacgttg
      781 agctttttag tggtaggttt atcctgtact tcttgtttgg atggcatcgt tgcaatttcc
      841 tgtcccttac caattgaaat atcagcaagg cttggctcag agctttccgt aggtgcaaac
      901 atatccagca caaaagaggt taaatcattt tcatcattta tgatgatttc tgagaacggt
      961 gcattatctg agcttgcctc tacctgagat atttcaactt ccttagaggt aataatagtc
     1021 tctggttgaa cattctctaa tgtaacaagt ttagtgcttt catcaactaa tggaggatga
     1081 ttttccatat tgctatcttc cattatctgc tcgactattt taactttccc ggaaaaataa
     1141 tttgggcgat cattaatatt ctcccaagcc acttcaggtt taattctcaa taatgtaaag
     1201 acgtttgccg gtgtccaacc tgaatcagct tgaatacggc aactccaaat cgccccacca
     1261 gaaggagatt cttctacgat accgagagat tgcatttcat caaataactt tggattatcc
     1321 ttaggcacgg aaatcccctg ccctagtaag taagttcgta tattatcagc ggcagttttg
     1381 ctaacaagcc acaatccttc ctcggtaaac caaccgtctg cagggccttt cggagtattt
     1441 aacttgattt tctgttccag taaataacgt agtgccaaga caagctgctt tgacagagaa
     1501 ttgacaggac gttgaaccag tttatttaca tcgccaccga gtgcgagggt aacactatac
     1561 tgatcagctt tttgcacgat ttcagataaa agcccagctt tatcattatg tccggacatt
     1621 gcatacatca gagaagaaat gacttctgga tactgtgcca gccaatcaaa tgcagatttg
     1681 ggaataatct gatttgcaaa gaagctgcct atcacaagat gaagatggta gtctcgacct
     1741 ttgatgtatt tgaatttata cggttcagga ggttctccat tccaagcaaa ccaacgtgag
     1801 ccatctttga gtgtgatttc aatatctact acaactttgc caatatcgtg aactaatgca
     1861 agataaattg ctgcggcagt ccaagcgtct cgctgctttg actgttcttc gggagccaca
     1921 ttcggtggca aaatataact ctggcgagct ttagctgaaa aagcaataac ttccaaaccg
     1981 tgatcgagca ttccaccaat atgagaatga tgatgtgatt ctgaagctgg caatagttga
     2041 accatttcag cgtaacgttc tattggttgc tgataaaatg tctcgaacat ttgacggttc
     2101 attgatacct gttgccaaag tagtccaaga tacttttgac gtaattccgt attcagtaat
     2161 tgagtagcat tttggggata tagccaacca tcgtcatctt gctcaataga gaaaacagtg
     2221 tcagatttga ccgcactttg agatgtgtgt ttaccaaaaa ttttcttgaa aattgattgc
     2281 attgaaagag accgaaaagt atcaattttg tgttaaatga aagccgttta gccctttgcc
     2341 cttttgggca agacctttcc ccttggaaag gttacctttc accctttagg ggttatcctt
     2401 tgcccttaag gactccttta tccttttgcc atttagggtg agcttggaat atattttgta
     2461 gtgtataaat atatttctgt taagttatct aaaaactctc aaatatttt
//
LOCUS       DANLYI010000243         2472 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.245, whole genome shotgun sequence.
ACCESSION   DANLYI010000243 DANLYI010000000
VERSION     DANLYI010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2472
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.245"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            139..1860
                     /gene="ptsI"
                     /locus_tag="PXD81_002345"
     CDS             139..1860
                     /gene="ptsI"
                     /locus_tag="PXD81_002345"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014992137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HDL5538325.1"
                     /translation="MITGIAASPGAVFGKALVLKEEPIVLNTRKITESEVEAEKAKFF
                     TGREKAAAQLTAIKEKARRTLGEEKEAIFEGHLMILEDEELEEEILSYLTDNLVTADV
                     AASKVIEMQASMLAEIDDEYLKERAGDIRDIGNRLVRNILNMNIVDLGDIQDEVILVA
                     YDLTPSETAQLNLDKVLGFITDVGGRTSHTSIMARSLELPAIVGTNNVTSLVKTGDFL
                     ILDAVNNEVHLNPSEEKIANFKALQEKLNAEKEELAKLKELPAQTLDGHKVDVAGNIG
                     TIRDVEGVLRNGGEAVGLYRTEFLFMDRTQLPGEEEQFQAYKEIVEAMNGQMVILRTM
                     DIGGDKELPYLDLPKEMNPFLGWRAVRIGFTRREILDTQLRAVLRASAFGNLAVMFPM
                     IISVEEIRLLKSIVAELKDQLRAEGHAFDENLKLGIMVETPSAAVNARHLAKEADFFS
                     IGTNDLTQYTLAVDRGNEIIAHLYNPLSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     VRATALLLGMGLDEFSMSGISVPHVKKLIRSINFADAKALADEALAQPTAADIERLVD
                     EFYQKLN"
     gene            1945..2445
                     /gene="crr"
                     /locus_tag="PXD81_002346"
     CDS             1945..2445
                     /gene="crr"
                     /locus_tag="PXD81_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005702996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDL5538326.1"
                     /translation="MGLFDKLFGSKQKESQEIKIYAPLTGEIVNIEDVPDVVFSEKIV
                     GDGVAIRPSGDTIVAPVNGTIGKIFETNHAFSIESEEGVELFVHFGIDTVELKGEGFT
                     RLAQEGQTVKVGEPIIKFDLALLEGKAKSVLTPIVISNMDEISNLSKLNGQVVAGESV
                     VLTLTK"
BASE COUNT          782 a          414 c          532 g          744 t
ORIGIN      
        1 ttcctacttt agaataattt ttctattctc ggatttaaga tttatgtcac tttattgtag
       61 cttaataagc tacaataaag tgttttgcta tgtaacatcc agatgatttt tacatcatct
      121 taaattaagg acacaattat gattacaggt atcgcagctt cccctggtgc agtatttgga
      181 aaagcattgg ttttaaaaga agagccgatt gtactaaata ctcgcaaaat tactgaaagc
      241 gaagttgagg cagaaaaagc aaaattcttt acaggccgcg aaaaagcagc tgctcaatta
      301 accgcgatta aagaaaaagc tcgccgtaca ttaggtgagg aaaaagaggc gattttcgaa
      361 ggccacttga tgatcttaga agatgaagag cttgaagaag aaattttgag ttaccttact
      421 gataatcttg tgactgcgga tgttgcagca agtaaagtga ttgagatgca ggcttcaatg
      481 cttgcggaaa ttgacgatga atatttaaaa gaacgtgcag gtgatattcg tgatattggt
      541 aaccgcttgg tacgcaatat tttaaatatg aatatcgtag atcttggtga tattcaagat
      601 gaagttattt tagtcgctta tgacttaacc ccttccgaaa cagctcaatt aaatttagat
      661 aaagtattag gctttattac cgatgtcggt ggtcgtactt ctcacacttc tattatggct
      721 cgttccctag agcttcctgc cattgttggt actaacaatg ttacttccct agttaaaact
      781 ggtgattttc tgattttaga tgcagtgaac aatgaagtac atttaaatcc atcggaagaa
      841 aaaatcgcta atttcaaagc attacaagag aaattaaatg cggaaaaaga agaattagcc
      901 aaattaaaag agttgcctgc tcaaacatta gatggccata aagttgatgt ggcagggaat
      961 atcggtacta tccgtgatgt ggaaggtgta ttacgcaacg gtggtgaagc ggttggttta
     1021 taccgtactg agtttttgtt tatggatcgt actcaattac cgggagaaga agagcaattt
     1081 caagcctaca aagagattgt tgaagcaatg aatggtcaaa tggtcattct gcgtacgatg
     1141 gatatcggag gtgataaaga gttaccatac cttgatttac cgaaagaaat gaacccattc
     1201 ttaggttggc gcgcggtgcg tatcggtttc acccgccgtg aaattttaga tactcagtta
     1261 cgtgcggtat tacgtgcttc tgcgttcggt aatttagcgg taatgttccc gatgattatt
     1321 tcggtggaag aaatccgttt attaaaatcc atcgttgcgg aattgaaaga tcaattacgt
     1381 gcagaaggtc acgcttttga tgagaactta aaactaggta ttatggtgga aaccccatca
     1441 gcagcggtaa atgcacgtca tcttgcaaaa gaagccgact tctttagtat tggtactaat
     1501 gacttaaccc aatatacact tgcggtagat cgtggtaatg aaattattgc tcatttatat
     1561 aacccattaa gcccatcagt cttaaactta atcaagcaag ttattgatgc ttcacacgct
     1621 gaaggcaaat ggacaggtat gtgtggtgaa ttagccggtg acgtgcgtgc aactgcgttg
     1681 ttgctcggta tgggattaga tgaatttagt atgagcggta tctctgtgcc acacgttaaa
     1741 aaactcattc gctcaattaa ttttgccgat gctaaagcat tagctgatga ggcgcttgct
     1801 cagccaactg ctgcagatat tgaacgctta gtagatgagt tttatcagaa attaaactaa
     1861 ttttaattat ttagcataca aatacaaaat tctcgtataa aatgtacgag aattttgtat
     1921 ctaatatcta agggagattt taagatgggt ttatttgata aattattcgg ctcaaaacaa
     1981 aaagagagtc aagaaatcaa aatctatgcg ccattaacag gtgaaatcgt aaatattgaa
     2041 gatgtgcctg atgttgtttt ctctgaaaaa attgtaggtg acggtgtggc aattcgcccg
     2101 agtggagaca ctatcgttgc tccagtaaat gggactatcg gcaaaatttt tgaaaccaac
     2161 cacgcttttt cgattgaatc tgaagaaggc gtggaattat ttgttcactt cggtattgat
     2221 actgttgaat taaaaggcga aggctttact cgccttgcac aggaaggtca aaccgtaaaa
     2281 gtgggcgagc cgatcattaa atttgattta gctctacttg aaggcaaagc caaatcagta
     2341 ttaacaccaa tcgtgatttc aaatatggat gaaatcagca acttatctaa attaaatggc
     2401 caagttgttg ccggtgaaag cgtggtttta accttaacca aataatacct cctacttagc
     2461 tggaagccac ga
//
LOCUS       DANLYI010000244         2450 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.246, whole genome shotgun sequence.
ACCESSION   DANLYI010000244 DANLYI010000000
VERSION     DANLYI010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2450
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.246"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            complement(96..380)
                     /gene="raiA"
                     /locus_tag="PXD81_002347"
     CDS             complement(96..380)
                     /gene="raiA"
                     /locus_tag="PXD81_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005823326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HDL5538327.1"
                     /translation="MSLNISSKQMDITPAIRTHIEERLAKLEKYHTQLINPHFMLIKQ
                     PNSFQVEATIGTPLGDLVAKAEHEDLYTAIFDVKKKLEGQLIKLKEKNEH"
     gene            560..1501
                     /locus_tag="PXD81_002348"
     CDS             560..1501
                     /locus_tag="PXD81_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005604585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxL/LpxP family Kdo(2)-lipid IV(A)
                     lauroyl/palmitoleoyl acyltransferasee"
                     /protein_id="HDL5538328.1"
                     /translation="MSEKKQLPPFQTQFLHPKYWGLWLGLGVFKLILCLPYPILVKIG
                     LGLGKLFTKLGFGKRRMRIARRNLELCFPHYSAEKVEELVEENMKSVGMAIIETGMAW
                     FWSDKRILKWSKIEGLEHLTQRDQERGILLVGVHFLTLELGARIIGLHHQGVGVYRPN
                     DNPLLDWIQFRGRVRSNKAMLDRKDVRGMIRALKNGEMIWYAPDHDYGRRNSVFVPFF
                     AVEETCTTAGSYMLLRSAPKSLVIPFSPIRNLDYSGYTVKVSPAVDFSQCQNEVETAI
                     LMNRVVEREIMAALTQYMWLHRRFKTRPNEGDQSLYD"
     gene            1597..>2450
                     /gene="dapA"
                     /locus_tag="PXD81_002349"
     CDS             1597..>2450
                     /gene="dapA"
                     /locus_tag="PXD81_002349"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HDL5538329.1"
                     /translation="MATPLFHGSIVALVTPMTHGEVDYQDLKKLVEYHIQAGSHAIVS
                     VGTTGESTTLSIDENVKVIKKTVEFADGRIPIIAGTGSNATSEAITMTKLLANSGVAG
                     CLSVVPYYNKPTQEGMYLHYKAIAESTDLPQILYNVPGRTGSDLKPETIGRLAKIENI
                     VAVKEATGDLSRLPLIKQLAGEDFIFLSGDDATGFESMKLGGQGVISVTNNVAAADMA
                     KMCELALAGQFTEAEEINNRLMALHHDLFVESNPIPVKWAAYKLGLISEPNLRLPLTT
                     LSESAQPVV"
BASE COUNT          750 a          384 c          515 g          801 t
ORIGIN      
        1 aatacggtaa attttaccgc ttactcttgg aataaaaaat agggctaaat gatatctctc
       61 atttagccct tattattaga tgtaaagttt aaacatcaat gttcattttt ctcttttagt
      121 ttaattaact gaccttctaa tttcttctta acatcaaaaa ttgcagtata taaatcttca
      181 tgttcagctt tagcgactaa atcacctaaa ggggtgccaa ttgttgcttc aacttggaat
      241 gagttaggtt gttttatcag cataaaatgt gggtttatca attgggtgtg gtatttttct
      301 aacttagcta aacgctcctc aatatgagta cggattgcag gggtaatgtc catttgtttg
      361 cttgaaatat ttaatgacat aacattttcc tcttgtttag ttaaggcttt tgccgttgat
      421 attctaataa ccaaaatatg ctcatcactc caaaaattca agtgaaaaat gaaataagtt
      481 tccaaaaatt tgatctactt aacactttta gcagttttta gctataatgt aatcataatc
      541 tagtatgtga gaataattaa tgagtgagaa aaaacagtta cctccattcc agacacaatt
      601 tttacaccct aaatattggg gattgtggct cggtttaggt gtttttaagt taattttatg
      661 tttgccttat cctattttag taaaaattgg tctgggacta ggtaaattgt ttactaaatt
      721 gggttttggt aaacgtcgta tgcgtattgc tcgccgtaat ttagagctct gttttcccca
      781 ttactctgct gagaaagtag aggaattagt cgaagaaaat atgaaatcgg ttggaatggc
      841 gattattgaa actggtatgg cttggttttg gtcggataaa cgtattctta aatggtctaa
      901 aatcgaaggg ctagagcatt taacacagcg tgatcaggaa aggggtattt tacttgtagg
      961 ggtgcatttt ctgactttag aactcggtgc aagaattatt gggctacatc atcaaggagt
     1021 aggggtttat cgaccgaatg ataatccatt attagattgg atacagtttc gaggacgtgt
     1081 tcgctcaaat aaagcgatgt tagatcgtaa agatgtgcgt ggaatgattc gagctttaaa
     1141 aaatggcgag atgatatggt acgcgcctga tcacgattat ggaagacgaa atagcgtttt
     1201 tgtaccattt tttgcagtgg aggaaacttg tactactgcg ggtagttata tgttacttcg
     1261 ttctgcccca aagagtttag tgatcccgtt tagcccgatt cgaaatttgg actattcagg
     1321 ctacacagta aaagtcagtc ctgccgttga ttttagtcaa tgtcaaaatg aagttgaaac
     1381 ggctatttta atgaatcggg tggtagaacg tgaaattatg gctgcgctaa ctcaatatat
     1441 gtggctacat cgccgtttta aaactcgccc aaatgaaggt gatcaaagcc tatatgatta
     1501 attttaaata atttcaaaaa agtgcttgca tatttaagct gaattttgca atctagcaat
     1561 gttatcaagc ataataaaaa aatacaggag atgattatgg cgaccccttt atttcacggt
     1621 agtatcgtag cattagttac accaatgact catggtgagg tagattatca ggatttaaaa
     1681 aaattagttg agtatcatat tcaagcaggt tctcacgcta ttgtatctgt tggtacaaca
     1741 ggtgaatcta ccacattaag cattgatgaa aatgttaagg tgattaagaa aacggttgaa
     1801 tttgctgatg gacgtattcc aattattgca ggaactggtt ctaatgcgac cagtgaagct
     1861 atcactatga ctaaattatt agcaaacagt ggtgtagctg gttgtttaag tgtagtgcct
     1921 tattacaaca agccaacgca agaggggatg tacttacact acaaagcgat tgcagaaagt
     1981 acagatttac cacaaattct gtataatgtg ccggggcgaa cagggagtga tttaaaacct
     2041 gaaacaatcg gtcgcttagc gaaaattgag aatattgttg ctgtgaaaga agcaacgggt
     2101 gatttaagcc gcttaccttt aattaagcaa ttagccggtg aagattttat tttcttaagt
     2161 ggtgatgatg ctaccggttt tgagtctatg aaattaggtg gtcaaggggt gatctcggtc
     2221 accaataatg ttgctgctgc tgatatggca aaaatgtgtg agttagctct tgccggtcaa
     2281 tttactgaag cagaagaaat taataaccgc ttaatggcgt tacaccatga cttatttgtc
     2341 gaatctaacc cgattccggt gaaatgggct gcttataaat taggtttaat tagtgagcct
     2401 aatttacgtt taccgcttac cacactgtct gaatcagcac agcctgtagt
//
LOCUS       DANLYI010000245         2415 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.247, whole genome shotgun sequence.
ACCESSION   DANLYI010000245 DANLYI010000000
VERSION     DANLYI010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2415
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.247"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            <1..412
                     /locus_tag="PXD81_002350"
     CDS             <1..412
                     /locus_tag="PXD81_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HDL5538330.1"
                     /translation="LEKFSKLQKYKCRIINVVDKTALVSKNSTLGIGVFVGKMAIVNS
                     GVTVGDNVIINTKSLVEHGCFIGSHCNISTNTTLNGDVIVEDHAFIGSSSVVNGQLRV
                     GESALVGSGAVVIRNVEPRTVVAGVPAKYIKDIK"
     gene            412..1449
                     /gene="neuB"
                     /locus_tag="PXD81_002351"
     CDS             412..1449
                     /gene="neuB"
                     /locus_tag="PXD81_002351"
                     /EC_number="2.5.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="HDL5538331.1"
                     /translation="MVNVFIVAEIGCNHNGDPALAKKMVDVAKECGVDAVKFQTFKAD
                     KLISKYAPKAEYQKVTTGTADSQLEMTRKLELPYEEFVKLEAYARSLGLEVFSTPFDF
                     ESIDFLASQQQKVWKIPSGELTNLPYLEKIARLPIEGKTIVISTGMATIEETKASLKV
                     LEDNGVAKKDITILHCNTEYPTPYEDVNLNAFHQLKQEFGDYSLGFSDHSAGYFAGLA
                     AVPYGITFIEKHFTLDKNFEGPDHKASVTPEELKLLCEGIRAVEKSLGSSEKLVTNSE
                     AKNKIVARKSIIAARPIKKGEIFTTENITTKRPGNGISPMFWYDVLGKEAQQDFEEDQ
                     LIQDSRFENQM"
     gene            1509..>2415
                     /locus_tag="PXD81_002352"
     CDS             1509..>2415
                     /locus_tag="PXD81_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase"
                     /protein_id="HDL5538332.1"
                     /translation="MKKIAIITARAGSKGLPNKNVLLANGKPLMAYSIEAAIESGQFE
                     KIIVSTDSQEYIDLFSHYPIEFIKRAEHLASDKASSFVVIEDVLNQYRSVEFDYFMLL
                     QPTSPLRSGEHIKESCEKFEKNFEQFDFLVSVSDAHKPTTLTRMIEEDECLKHFQLDY
                     SNYARQLHHPEYSPNGAIFTAKPEAYLKQKHFYGAKCLAYFMDKDVSIDIDDRQDFEY
                     FYFILQQRNKEKILLETIKRSILMKKENFKEVKEIALIGHSIIEYWDIEHLNGKAVNN
                     LGIAGISTKQYSDLILDEGLISALPK"
BASE COUNT          794 a          394 c          510 g          717 t
ORIGIN      
        1 tttagaaaaa ttctctaagc tacaaaaata taagtgtaga ataattaatg tggtagataa
       61 aaccgcattg gtgtccaaaa attccactct aggcattgga gtgtttgttg gaaaaatggc
      121 aattgtaaac agtggcgtaa ctgtaggcga taatgtgatc attaacacta aatcgctggt
      181 tgagcatggt tgttttatcg gcagtcattg caatatttca accaatacga cactaaatgg
      241 cgatgtaatt gtcgaagatc atgcttttat tggtagttcc tctgttgtga acggacagct
      301 aagagtgggt gaaagtgcat tggttggctc cggtgcagtg gtaattagaa atgtggagcc
      361 tcgcacagtt gtagcaggcg ttccagcaaa atatattaag gatataaaat aatggtaaat
      421 gtatttattg tggcagaaat cggttgtaac cataacggcg atcctgcgtt agcaaagaaa
      481 atggttgatg tagcgaaaga gtgtggtgtc gatgcggtga aattccaaac ctttaaagcg
      541 gataagttga tctctaaata tgcccctaaa gccgagtatc aaaaagtaac gaccggcacc
      601 gctgattccc aattagaaat gacccgcaaa ttagagttac cttatgagga gtttgtgaaa
      661 ttagaggctt acgctcgttc attggggtta gaggtattct ctacgccatt cgatttcgag
      721 tcgattgatt tcttggcaag ccaacaacaa aaagtgtgga aaattccgtc tggtgaatta
      781 accaatttgc cgtatttaga aaaaattgca cgacttccga ttgaaggcaa gacgatcgtt
      841 atttctaccg gtatggcaac cattgaagaa acaaaagcct cattaaaagt attggaagat
      901 aacggtgtag cgaaaaaaga tattacgatt ttacattgta ataccgagta cccgacacct
      961 tatgaagatg tgaatttaaa tgcgttccat cagctaaaac aggagtttgg cgactatagt
     1021 ttaggttttt ctgaccactc tgccggttat tttgcagggt tggcggcagt accttacggc
     1081 attaccttca ttgaaaaaca ttttacctta gataaaaact ttgaaggtcc ggatcataaa
     1141 gcttcggtta cacctgaaga gctgaaatta ctgtgtgaag gcattcgtgc ggtggaaaaa
     1201 tcgctgggtt cttccgagaa attagtcact aattcggaag caaaaaataa aattgtggca
     1261 agaaaatcaa ttattgcggc acgtccaatt aaaaaaggcg aaatttttac aaccgaaaat
     1321 attacgacta aacgccccgg taatggcatt agcccgatgt tttggtatga tgtgctaggt
     1381 aaagaagcac aacaggattt tgaagaagat caattaattc aagattctcg ttttgaaaat
     1441 cagatgtaat ataatatagt gatgggatga aagctccatc attttgtgtt taacaagcgg
     1501 tcaaatttat gaaaaaaatt gcaattatta cagcccgagc cggttcaaaa ggcttgccga
     1561 ataaaaatgt attattagcc aacggtaagc cgttaatggc ctattctatt gaagctgcga
     1621 ttgagtcggg gcaatttgag aaaattatcg ttagtactga ttcccaagaa tacattgatt
     1681 tgttctcaca ttatccaatt gaatttatta aacgtgccga gcatttagcc agtgataagg
     1741 cgagttcttt tgtcgtgatt gaagatgtgt taaatcaata tagatcagta gagtttgact
     1801 attttatgct acttcaacca acttcgccgt tgagatcagg agagcatatt aaggaatcgt
     1861 gtgaaaaatt tgagaaaaat ttcgaacaat ttgatttctt agtatctgtt tctgatgccc
     1921 ataaacctac gacattgact cgtatgattg aggaagacga gtgcctaaag cattttcagt
     1981 tggattattc taactatgca cgccaattgc atcatcctga atattcaccg aatggagcta
     2041 tttttacagc taagccagaa gcctatctta aacaaaaaca cttctatggt gcgaaatgtc
     2101 tagcctattt tatggataaa gacgtttcta ttgatattga tgatcgccaa gatttcgagt
     2161 acttctactt tattctgcaa caacgcaata aagaaaaaat cttacttgaa accattaaac
     2221 gttcgatttt aatgaaaaaa gagaacttta aagaagtgaa agagatagcc ttaatcgggc
     2281 attcgatcat tgaatattgg gatattgaac atttaaacgg taaggcagtt aataatttag
     2341 gaattgcagg tatttcaaca aaacaataca gtgatctgat tttggatgaa ggtttaattt
     2401 ctgctttacc taaag
//
LOCUS       DANLYI010000246         2407 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.248, whole genome shotgun sequence.
ACCESSION   DANLYI010000246 DANLYI010000000
VERSION     DANLYI010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2407
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.248"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            126..2402
                     /gene="gshAB"
                     /locus_tag="PXD81_002353"
     CDS             126..2402
                     /gene="gshAB"
                     /locus_tag="PXD81_002353"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HDL5538333.1"
                     /translation="MKLQKLIRENHLALLFQQGNFGLEKESQRVDRNGNIVTTPHPAV
                     FGNRSYHPYIQTDFAESQLELITPPNAKLEDSFRWLSAIHEVVLRSLPEEEYIFPLSM
                     PAGLPPEEQIQEAQFDNPEDVQYRQHLSKNYGKYKQMVSGVHYNFQLSTEFVEKAFAL
                     QTEYSELKAFQNALYMKLANNFLRYQWILLYLLAATPTVESQYFYEKSPLARGQLVRS
                     LRSSPYGYVNSEKVVVNHDSLESYVESLEANVANGLLIAEKEFYSNVRLRGGKKAREL
                     LENGVKYAEFRLFDLNPFAPYGIELNDAKFIHYFLLGMLWLEETSGQKEVEIGKQKLY
                     QVALEDPRTETAFRAEGEAILNQLLAMLEELSAGQESLDLVREKLAQFADPSKTLNGR
                     LIAAIEEVGSYKALGAKLAQTYKAQAFERFYALSAFDNMELSTQALFFDLIQQGIEVE
                     VLDENDQFLALKFGDHLEYVKNGNMTSRDQYISPLIMENKVVTKKVLAKAGFNVPKSV
                     EFTNLEQAVAHYPLFEGKAVVIKPKSTNYGLGITIFQQAVKNREDFAKAVEIAFREDK
                     EVMVEDYLVGTEYRFFVLGDETLAVLLRVPANVVGDGIHSVKELVERKNDDPLRGDGS
                     RSPLKKIALGEIEQLQLKEQGLTVDSVPAKGQTVQLRANSNISTGGDSIDMTDQMHES
                     YRQIAVGIAQAMGAKVCGVDLIIPDLTKPAEPNLSSWGVIEANFNPMMMMHIFPYQGK
                     SRRLTKAVIKMLFPEIGN"
BASE COUNT          730 a          494 c          540 g          643 t
ORIGIN      
        1 gaagcagtat ttctgttata accaaatcat ctacttcatt ccaataaact aaaacccact
       61 atttcactaa gtctttcatt tgtgttattg tgccgataca tttttttgac tacaaaggat
      121 agatgatgaa actacaaaaa cttatccgag aaaatcattt agcattgctg tttcaacaag
      181 ggaattttgg gcttgagaaa gagagccaac gggttgatcg caacggcaat atcgttacta
      241 ctccccaccc tgccgtattc ggtaatcgct cttaccaccc ttatattcaa accgattttg
      301 ccgaaagtca gctagagtta atcactcccc cgaatgctaa attagaagac agttttcgtt
      361 ggctctctgc tattcacgaa gtggtattgc gttcgttgcc agaggaggaa tatatcttcc
      421 ctctcagtat gccggcaggc ttgccgccgg aagagcaaat tcaagaagca caatttgaca
      481 accctgaaga tgtgcaatat cgccaacacc tttccaaaaa ctatgggaaa tataagcaaa
      541 tggtaagcgg cgtgcattat aattttcaac tttcaacgga atttgtcgaa aaagcctttg
      601 ctttacaaac ggaatatagc gagctgaaag ccttccaaaa cgcactttat atgaagttag
      661 ctaataactt tttgcgttat caatggattt tgctctattt attggctgcc acgcctacgg
      721 tggaatcgca atatttttat gaaaaatcac cgcttgcgag agggcaactt gtccgcagct
      781 tgcgttcaag cccttatggt tatgtgaatt cggaaaaagt ggtggttaat cacgacagct
      841 tagaaagcta tgttgaaagc cttgaagcca atgttgccaa cggattatta attgccgaaa
      901 aagaattcta ttccaatgtg cgtttacgag ggggcaaaaa agcccgtgag ctattggaaa
      961 acggtgtgaa atacgccgaa ttccgtttat ttgatttaaa ccctttcgcc ccttacggca
     1021 ttgagctgaa tgatgccaaa tttatccatt atttcttact cggtatgctt tggctggagg
     1081 aaacaagcgg tcaaaaagag gtcgaaatcg gcaaacagaa actttaccaa gtcgcacttg
     1141 aagatccaag aacagaaacc gctttccgtg ccgaaggtga agccatttta aatcaacttt
     1201 tagcgatgct tgaagaatta tctgccggtc aggaaagcct agatttggtg cgggaaaaat
     1261 tagctcaatt tgctgatcca agcaaaaccc tgaacggacg tttaattgcc gcaatagagg
     1321 aagtcggcag ttataaagca ttgggggcta aattggctca aacctataag gcacaagcct
     1381 ttgagcgttt ttatgcgtta tcggcatttg ataatatgga actttccact caagccctgt
     1441 tttttgattt gatccaacaa gggattgagg tggaagtact ggacgaaaac gatcaattcc
     1501 tcgccctaaa atttggcgat catttggaat atgtgaaaaa cggcaatatg accagccgag
     1561 atcaatacat ttcaccgctg attatggaaa acaaagtggt gacgaaaaaa gtattggcaa
     1621 aagccggctt taacgtgccg aaaagcgttg aatttaccaa ccttgaacag gctgtggcac
     1681 attatccgct atttgaaggc aaagcggtgg tgattaagcc gaaatcgact aactatggtt
     1741 tagggattac catcttccaa caagcggtca aaaatcgaga agattttgca aaagcggttg
     1801 aaattgcgtt ccgtgaagac aaagaggtga tggtggaaga ctatttagtc ggtactgaat
     1861 accgcttctt tgtactgggc gatgaaaccc ttgcggtgtt actgcgtgta ccggcgaatg
     1921 tagtgggaga tggcattcac agtgtaaaag agctggtgga gcgtaaaaat gacgatccgc
     1981 tacgtggcga tggctctcgt agcccgttga aaaaaattgc cttaggtgaa attgagcagt
     2041 tacaacttaa agagcaaggc ttaaccgtag attccgtacc ggcgaaaggt caaacagttc
     2101 aactgcgtgc caattccaac atcagcaccg gcggcgattc gattgatatg accgaccaaa
     2161 tgcacgaaag ctatagacaa attgcagtgg gaattgctca ggcaatgggg gctaaagtgt
     2221 gtggggtgga tttgatcatt cctgatctca ccaaacctgc cgagccaaat ttaagctctt
     2281 ggggcgtcat tgaggcaaac ttcaacccaa tgatgatgat gcacattttc ccttaccaag
     2341 gcaaatcccg ccgtttaacc aaagcggtaa tcaaaatgct gtttcctgag ataggcaatt
     2401 aaatttt
//
LOCUS       DANLYI010000247         2392 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.249, whole genome shotgun sequence.
ACCESSION   DANLYI010000247 DANLYI010000000
VERSION     DANLYI010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2392
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.249"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            <1..334
                     /locus_tag="PXD81_002354"
     CDS             <1..334
                     /locus_tag="PXD81_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HDL5538334.1"
                     /translation="DELVTINEKIVNLTYGDQDALNIYFKGGWGALPIEYNYQVDAIL
                     ELVLRREQEELARKNGYLDVIPKIIHYTSKFKPWKKELHTMQISTRKKYWFYYHLEWN
                     DILEQHQK"
     gene            complement(380..667)
                     /locus_tag="PXD81_002355"
     CDS             complement(380..667)
                     /locus_tag="PXD81_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HDL5538335.1"
                     /translation="MKNDKPIECEGCNTFDVGSILDNSETVATIQRTYESENEAKAAL
                     EKFTQKARDVESEPCEISSEITPIESGFELVAHFKFSCQAEVVIFQLGTRF"
     gene            787..1200
                     /locus_tag="PXD81_002356"
     CDS             787..1200
                     /locus_tag="PXD81_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PACE efflux transporter"
                     /protein_id="HDL5538336.1"
                     /translation="MTAWERVFHALLFECFAIIFTVILTSWLTSHRMVDLTAVIVMIS
                     VIAMVWNVIFNWGFDKVFTGERVQRGLGIRILHSFLFEGGLLVFTIPLVMYMLDIGWW
                     QAFVMDIGMTLFVLVYSVVFNWVYDHLRLRWVKSG"
     gene            1285..2277
                     /gene="mglB"
                     /locus_tag="PXD81_002357"
     CDS             1285..2277
                     /gene="mglB"
                     /locus_tag="PXD81_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HDL5538337.1"
                     /translation="MKRTILSAFALSVICGVNLSYAQSESRVGVTIYKYDDHFMTQMR
                     KDMQNEAKGFAGLKWFMNDAQNSQVKQLAQIETLLQHKMRVLAVNIVNANESKTIVEK
                     AKAYNVPVVFFNRTPSKQALESYEKAYFVSADPKEAGRIQGELIAKVWKSNPDFDLNK
                     DGKLQFVLLKGEPSDVSAERSQAVVESLNQQGVQAEEIYSDTARWRRTLARNKMNEWL
                     VENRAAEIEVVISNNDEMAIGALDALNAHEKRLPIFGIDALPETLALMKKGEIAGTVL
                     NDSSAQAKAVVKLVSNLAQGKPALKDTQWESHSSKTIYLPHIGIDKENLGQFLP"
BASE COUNT          711 a          377 c          556 g          748 t
ORIGIN      
        1 tgatgagttg gttactatca atgaaaaaat tgtaaactta acttatggcg atcaagatgc
       61 tctcaatatt tactttaaag gcggttgggg agcattgccg atagagtata attatcaagt
      121 tgatgcaatt cttgaactag tattaagaag agaacaagag gaacttgcga gaaaaaatgg
      181 ctatttagat gtgattccga aaattatcca ctatacgagt aaattcaaac cttggaaaaa
      241 agaactgcat acaatgcaaa tatctacgcg taaaaaatat tggttttact atcatttaga
      301 gtggaacgat attttggaac aacatcaaaa atagctatca tacaaaagct gattcaagtg
      361 tttgaatcag ctttttgtat tagaaacgtg ttcctaattg aaaaataacc acttctgctt
      421 ggcagctaaa tttgaagtga gcaaccagtt caaaaccgct ttcaatcggg gtgatttcac
      481 tgctgatttc acagggttca gattcgacat cacgggcttt ttgagtgaat ttttcaagtg
      541 ccgctttagc ttcattttcg ctttcataag tgcgctgaat agtagcgact gtttcgctgt
      601 tatctaaaat agaacctaca tcaaatgtgt tacagccttc gcattcaatc ggtttatcgt
      661 ttttcattgg ctaatccttc taaattgatg aataatgtag gtactttaaa actaaattgc
      721 gtaacctgca atcatcagtg aacgattatt tgatttagtt ggtattttgc aaagtttgga
      781 ggaaaaatga ccgcttggga gcgtgtgttt cacgcgctgt tatttgaatg tttcgctatt
      841 atttttacgg ttatcttaac ttcttggctg acttctcatc gtatggttga tttaaccgca
      901 gtgattgtga tgatttcggt gattgcaatg gtgtggaatg ttatttttaa ctggggtttt
      961 gataaggtgt ttaccggtga gcgagtgcaa cgtggtttag gcattcgtat tttgcattca
     1021 ttcctttttg agggcggttt gctggttttt actatcccat tggtaatgta tatgctcgat
     1081 atcggctggt ggcaggcgtt tgtgatggat attggaatga ccttatttgt attagtttat
     1141 tcggtagttt ttaattgggt gtacgatcat ttacgcctga ggtgggttaa atctggctaa
     1201 attgtgaact agatcacggt ttccttacac aaaattcgct atgcttgaat tggttcatat
     1261 ttggcatagc aagagggaca ttctatgaag cgtacaatct tgagtgcttt tgcactttct
     1321 gttatttgcg gtgtgaatct aagttacgca caatctgaaa gcagagtggg ggtaacgatt
     1381 tacaaatatg atgaccattt tatgactcaa atgcgtaagg atatgcaaaa cgaggcgaaa
     1441 ggcttcgctg ggttgaagtg gtttatgaat gatgcacaaa attcccaagt gaagcaatta
     1501 gcgcaaattg aaaccttact tcagcataaa atgcgtgtgt tggcggtgaa tattgtgaat
     1561 gcaaatgagt cgaaaactat tgtggaaaaa gcgaaggctt ataatgtgcc ggttgtcttt
     1621 tttaaccgca caccgagtaa acaagcgtta gaaagttacg aaaaggcgta ctttgtgagc
     1681 gctgatccta aagaggccgg tagaatccaa ggggaattaa ttgcgaaagt gtggaaatca
     1741 aaccccgatt tcgatctaaa taaagacggg aaattgcaat ttgtattgct taagggcgag
     1801 ccgagtgatg tgagcgctga gcgttctcaa gctgtggttg aatcgcttaa tcaacaaggt
     1861 gttcaggcag aagaaattta cagtgacacg gcgagatggc gtagaacatt ggctcgcaat
     1921 aaaatgaatg agtggctggt ggaaaaccgt gcagctgaga ttgaagttgt gatttcaaac
     1981 aatgatgaaa tggcaattgg tgctttagat gcgttaaatg cacacgagaa aagattgccg
     2041 atctttggta ttgatgcctt gcctgagacg ttagcattaa tgaaaaaagg ggaaatagcc
     2101 ggcacggtat taaatgatag ctctgcacaa gcgaaagcgg tggtgaaatt ggtatctaat
     2161 ttggcacaag gtaagcctgc gctgaaagat acgcagtggg aaagccattc ctctaaaacg
     2221 atttatcttc cacatattgg tattgataag gaaaatttag ggcaattttt accttaaatg
     2281 gaggagcggg gcgttataaa tacgcccctt attgatgata acaagcggtc atttttcagt
     2341 aaaatcttgc aaatcctttc tcttttcttc caacagattt gaatagagcg aa
//
LOCUS       DANLYI010000248         2389 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.250, whole genome shotgun sequence.
ACCESSION   DANLYI010000248 DANLYI010000000
VERSION     DANLYI010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2389
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.250"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            <1..1882
                     /locus_tag="PXD81_002358"
     CDS             <1..1882
                     /locus_tag="PXD81_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HDL5538338.1"
                     /translation="NGDIPHIQCLVRKKDIKLTPEEAVRQLYLMNLHYEYDYPFERME
                     LEYSVVFGREKKRADIVIFDRDNPNAVYIMVELKKPKLKDGKEQLKSYCNATGAPIGV
                     WTNGESISYYNRRDPNYFKDIPDIPNAQQKLSDLLQERWTIQDLIDNDKIAKQRKSLK
                     DLVIEMEDEVLANAGVDVFEELFQLIFTKLYDEMESTRNKSRYLEFRNYGDTESELKA
                     KIQSLFDRAKNKWEGVFAESAKINLSPSHLAICVSSLEEVKLFNSNLDVVDEAFEYLI
                     NKSSKGEKGQYFTPRYVIDMCVKMLDPKPEETVIDTAAGSCGFPVHSIFHVWEKQLKA
                     RGLERSHLFTAEEKLPEQTDYVKEKVFAIDFDEKAVRVARTLNLIAGDGQTNVLHLNT
                     LDYERWEDFTKEEEWNDVYGEGWKKLRKLRKTKNENRDFQFDVLMANPPFAGDIKETR
                     ILARYELGKNSKGKQQSKVGRDILFIERNLDFLKDGGRMAIVLPQGRFNNSSDKYIRD
                     FIAERCRILAVVGLHGNVFKPHTGTKTSVLFVQKWHDELCPKVEDYPIFFATMQKSSK
                     DNSGDKIYVKNADGTLKLDNHQHLIVEHDLFNHDNSTQDGIAEAFIEFAKKEKLSFVG
                     KD"
     gene            1981..>2389
                     /locus_tag="PXD81_002359"
     CDS             1981..>2389
                     /locus_tag="PXD81_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HDL5538339.1"
                     /translation="MDSTFFLKKYLNHPSFNFPLSLNEVSLIKSGTTPTDRDDNLKEG
                     VVLLKTNDIRNNLLNKYSSVDYFISEDINEKMISSQLKEGDVLVNIVGATLEVVGRVA
                     YVSSTFPKANITQAMSFVRLKSKYNKELLPTYLF"
BASE COUNT          832 a          366 c          477 g          714 t
ORIGIN      
        1 aaatggagat attcctcata ttcaatgtct tgttcgtaaa aaggatatta agctcacacc
       61 tgaagaagca gtacgccaac tctatttaat gaatttgcat tatgaatatg attatccatt
      121 tgaacgaatg gaattagaat attctgttgt attcgggcgt gagaagaaac gagcggatat
      181 tgtcatcttt gatagagata atcctaacgc tgtctatatt atggtggagc tgaaaaagcc
      241 gaaattaaaa gatggtaaag aacaattaaa atcttattgt aatgcgactg gagcacctat
      301 tggtgtatgg acaaatggcg aaagtatttc ttactataat cgccgtgatc ctaattattt
      361 taaagatatt cccgatattc cgaatgctca acaaaaactt tctgatttat tacaagaacg
      421 ttggactatt caagatctga tagataatga caaaattgcc aaacaacgta aatcattgaa
      481 agatcttgtt attgaaatgg aagatgaggt actggcgaat gctggagttg atgtttttga
      541 agagttattt caacttattt ttactaaact ctatgatgaa atggaaagta cacgcaataa
      601 aagtcgttat ttagagtttc gtaactatgg tgataccgaa agtgaattaa aggcaaaaat
      661 tcaatctcta tttgatcgtg caaaaaataa atgggaaggc gtatttgctg aaagtgccaa
      721 aatcaacctt tccccttcgc atttggcgat ttgtgtatct tcattggaag aagtaaagct
      781 gtttaattct aaccttgatg tagtggatga agcatttgag tacttaatta ataaatccag
      841 taaaggagag aaaggacaat actttacgcc tcgttacgtc attgatatgt gcgtaaaaat
      901 gctcgatcca aaacctgaag aaacagtcat tgataccgca gcaggaagct gtggttttcc
      961 agttcattct atttttcacg tttgggaaaa acaacttaaa gcgagaggat tagaacgctc
     1021 acacttattt actgctgaag aaaaattgcc tgaacaaaca gattatgtga aagaaaaagt
     1081 ctttgcgatt gatttcgatg aaaaggcagt acgtgttgct cgtaccctaa acttaatcgc
     1141 aggagatggg caaaccaacg tactacattt aaatacctta gattatgaac gttgggaaga
     1201 ttttactaag gaagaagaat ggaatgatgt atatggagaa ggctggaaaa aattaagaaa
     1261 attacgtaaa actaaaaatg aaaatcgaga tttccaattt gatgtgctaa tggcaaatcc
     1321 gccttttgcc ggtgatatta aagaaactcg tatattagct cgctatgaat taggcaaaaa
     1381 tagcaaagga aaacagcaat ccaaagtcgg acgagatatt ttatttattg aacgcaattt
     1441 agatttctta aaagacggtg gacgaatggc gattgtgcta ccacaagggc gttttaacaa
     1501 ttccagcgat aaatatattc gtgattttat tgcagagcgt tgccgcattt tagctgttgt
     1561 gggcttacac ggaaatgtgt ttaaaccaca taccggcaca aaaacttccg tactatttgt
     1621 gcaaaaatgg cacgatgaac tttgcccgaa agtagaggat tacccgattt tctttgccac
     1681 aatgcagaaa tccagcaaag ataattcagg cgataaaatc tatgttaaaa acgctgatgg
     1741 cacactcaaa ttagacaacc atcaacacct aattgtggag cacgatctat ttaatcacga
     1801 taatagtacc caagacggca ttgcagaagc ctttattgaa tttgctaaaa aagaaaagct
     1861 ctcttttgtg ggaaaggatt aagcccattt gaccctgcta aatatcaggc attgttagat
     1921 gggctagagg tgagcgaggt ttatttgagt aaggttcgtt ctgcaagtta ttcggaacga
     1981 atggatagta ctttcttttt gaaaaaatac ttaaaccatc cgagttttaa ttttccatta
     2041 tcgctgaatg aggtttctct gattaaaagt ggtacaacac caacagatcg agatgataac
     2101 ttaaaagagg gggttgtctt attaaaaacc aatgatatta ggaataatct attaaataag
     2161 tactcttcgg tagattattt tattagcgaa gatatcaatg agaaaatgat ttcatcacaa
     2221 ctgaaagaag gggatgtttt agtaaatatc gttggtgcaa ctttggaggt tgttggtcgg
     2281 gtagcttatg ttagtagcac ttttccaaaa gcaaatatta cacaagcaat gtcttttgta
     2341 agattaaaat caaaatataa taaagaatta ttaccaacgt atttatttg
//
LOCUS       DANLYI010000249         2331 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.251, whole genome shotgun sequence.
ACCESSION   DANLYI010000249 DANLYI010000000
VERSION     DANLYI010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2331
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.251"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            <1..144
                     /locus_tag="PXD81_002360"
     CDS             <1..144
                     /locus_tag="PXD81_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HDL5538340.1"
                     /translation="KKKIHNLISRAHYDMATNEDSASEHLELFGEDIAEDSFDYPKPE
                     KLI"
     gene            151..222
                     /locus_tag="PXD81_002361"
                     /pseudo
     CDS             151..222
                     /locus_tag="PXD81_002361"
                     /inference="COORDINATES: protein motif:HMM:NF013704.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            232..750
                     /locus_tag="PXD81_002362"
     CDS             232..750
                     /locus_tag="PXD81_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HDL5538341.1"
                     /translation="MNRRYIGIEIGEHAKTHVVPRLKKVIEGEQGGISKAVNWQGGGS
                     FRFCELGEEVFDAFGSLNPNIRFDDLAAHIWYLENHFPLQKNSEKSPLVGTFNGKAYY
                     LLYNGILGDKRPQSGNVLTRKLMAELPYFAEFIQQGMDIVVYGEACRLGEVQLAEKKI
                     TFKQIPYDVMSR"
     gene            766..1755
                     /locus_tag="PXD81_002363"
     CDS             766..1755
                     /locus_tag="PXD81_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HDL5538342.1"
                     /translation="MKLKNYQNEALNRVSHYFSACLQSDAKTAFSQIQPEYSYKIPSE
                     HSNLRDVPYVCVRIPTGGGKTLLASHSIARIAKQYLDCDFPVTLWPVPSRTIKTQTTE
                     ALKNPQHPYRAVLDKTFNREVMVIESEDFDLLRPQDFGNKAIVIVSTIQNFRVENQDG
                     RKIYAFNEKLTAHFERLLPHIQANLEKISTTDLQENGLTSKQTGKIKCSFANLLRAYQ
                     PLVIVDEAHNARTSLSFDVLSQLSPSAILEFTATPNTDRKAGSNVLYHVSASHLKAEE
                     MIKLPIVLTEHHNWQQAIDEAVINRGALAQKAQYESEYVRPIYCFKLSRKTAK"
     gene            1722..2198
                     /locus_tag="PXD81_002364"
     CDS             1722..2198
                     /locus_tag="PXD81_002364"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5538343.1"
                     /translation="MFQAEPKNGKVTVEVLKQYLIDELKIDEEEIAIVIGNQHELDNI
                     DLFNPQCKINYIITVEALKEGWDCPFAYIFCSVQNVSSSKEAEQLLGRVLRMPYAKHR
                     QIEDLNRAYAPLSSKSFGETARNLQDKLIGMGFEALEVAEMLRIPQQENWEGFLYH"
BASE COUNT          762 a          403 c          476 g          690 t
ORIGIN      
        1 aagaaaaaaa ttcataactt aattagcaga gctcattatg atatggcaac aaatgaagac
       61 tctgctagtg aacatctaga attatttggg gaggatattg cagaagattc atttgattat
      121 cctaaacctg aaaaattaat ctaatttgta ttagaagcat cgactgaacc atatgatctc
      181 gtcctcgata gctttcttgg ctcaggtaca actgcagcag ttgctcataa aatgaaccgt
      241 cgttatatcg gtattgagat tggcgaacat gctaaaaccc atgttgtccc acggttgaaa
      301 aaagtaattg aaggtgagca aggcggtatt tcaaaagcag taaattggca aggtggtgga
      361 tcgtttcgct tttgtgagtt aggcgaggaa gtatttgacg cattcggttc acttaatccg
      421 aacattcgtt ttgacgattt agccgcccat atttggtatt tagaaaatca tttccctttg
      481 caaaaaaaca gcgaaaaatc accgcttgtc ggcactttca acggcaaagc ctactatttg
      541 ttatataacg gaattttagg cgataaacgt ccgcagagtg gtaatgttct gacacgaaaa
      601 ttaatggcgg agttaccata ctttgctgaa tttattcaac agggaatgga tattgtggta
      661 tatggcgaag cgtgccgttt aggagaagtg cagttagctg aaaagaaaat tacatttaaa
      721 cagattccat atgatgtaat gtctcgttaa tagtaggaga aaaaaatgaa attaaaaaat
      781 taccaaaatg aggcattaaa ccgtgtcagc cactattttt cggcttgttt gcaatctgat
      841 gcaaaaaccg catttagcca aattcaacct gaatatagtt acaagatccc aagtgaacac
      901 agtaatttgc gtgatgtgcc ttatgtttgt gtacgaattc caactggtgg cggtaaaacg
      961 ttgttagcct cgcatagtat tgctcgtatt gcaaagcagt atttggattg cgatttcccg
     1021 gttaccttgt ggccagtgcc ttctcgtaca attaaaaccc aaactacaga agcattaaaa
     1081 aatccgcaac acccttaccg tgcagtactt gataaaactt ttaaccgaga agtaatggtt
     1141 attgagtctg aagattttga tttacttcgc ccacaagatt ttggtaataa ggcgattgta
     1201 atcgtttcta ctattcaaaa ttttcgggta gaaaatcaag atgggcgaaa aatctatgct
     1261 tttaatgaaa aattaacagc tcattttgag cgtttactac cccatattca ggcaaattta
     1321 gaaaagattt ccactactga tctacaagag aacggtttaa cctccaaaca aacgggcaaa
     1381 attaagtgtt cttttgcaaa tttgttgcga gcttatcagc ctttggtcat tgttgatgaa
     1441 gcacataatg ctcggacttc actctctttt gatgtgctat ctcaactttc cccatcagct
     1501 attttagaat ttactgctac gccaaacaca gatagaaaag caggaagtaa tgtgttgtat
     1561 cacgtttcgg caagccatct aaaagcagaa gagatgatca aattgcctat tgtgctgacc
     1621 gaacatcaca actggcaaca agcaatagat gaggctgtaa tcaatagagg agcattagca
     1681 caaaaagcac agtatgagag cgagtacgtt cgtccgattt attgtttcaa gctgagccga
     1741 aaaacggcaa agtaacagta gaagtcttaa aacagtattt aattgatgag ttaaaaattg
     1801 atgaagaaga gattgccatt gtgataggta atcaacacga attagataat atcgatttat
     1861 ttaatccaca atgcaagatc aattacatca tcacggttga ggcattaaaa gaagggtggg
     1921 attgcccttt tgcttatatt ttttgctcag tacaaaatgt ttcctctagc aaggaagcgg
     1981 agcagctatt agggcgagta ttgcgaatgc cttatgctaa acaccgtcaa attgaagatt
     2041 taaatcgtgc ttatgctccg ctttcctcta agtcgttcgg cgaaacggct cgtaatttac
     2101 aagataaatt gattggaatg gggtttgaag cattagaagt agctgaaatg cttcgtatcc
     2161 cacaacaaga aaattgggag ggttttcttt accattagct catcagaatt tttaccctga
     2221 ttttattgta gagcttattg atggtcgaat tttaattgtg gaatataaag gggaacaatt
     2281 aaaaagtaat gaagattcta aagaaaaagc cttaattggc aatctttggg a
//
LOCUS       DANLYI010000250         2279 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.252, whole genome shotgun sequence.
ACCESSION   DANLYI010000250 DANLYI010000000
VERSION     DANLYI010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2279)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2279)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2279
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.252"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            126..1280
                     /locus_tag="PXD81_002365"
     CDS             126..1280
                     /locus_tag="PXD81_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HDL5538344.1"
                     /translation="MNQFFPLIFSLTLFSGVTLALEKAPRTMPTEGINKPLLGQILFF
                     DNMLSHNGSQNCSSCHNPDYAFIDNRTTPAKGVVSQGDTPTKFGKRNSPTMLYAKYSP
                     EFHFDEQSQEYIGGQFWDGRAKNLAEQAGMPPIDPNEMGMPNKLEVAKRLYNVPMYQR
                     FLGQFYGEKVWESYESVYAAMEDAIATFQQENKLLAPFDSKYDLFLKGEVQLTALEEK
                     GRALFFNKAQTNCSSCHQLQSAANHQEETFSNYHYYNVGVPGNPDLIAVNGFAPDFVD
                     LGLFENPNVKGDETQKGKFKTPTLRNVAVTALYMHNGVFKELKTVLLFLDSYNNPERK
                     INPETGTAWQQAEFEPTIAHQKLKAKPLSDEEIDALEAFLKTLTDERYRK"
     gene            complement(1328..>2279)
                     /locus_tag="PXD81_002366"
     CDS             complement(1328..>2279)
                     /locus_tag="PXD81_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="virulence RhuM family protein"
                     /protein_id="HDL5538345.1"
                     /translation="SNVALLVVENEAWLTQNQLAELFDTSVQNISEHIKNILKDKELD
                     ENSVIKDYLITAQDGKNYQVKHYSLEMILAIGFRVRSPRAVQFRKWANSTLRTYLEKG
                     FLIDSERLKNPQGRLDYFDELLEQIREIRASELRFYQKVRELFKLSSDYDKTDKNTQM
                     FFAETQNKLIYAITKHTAAELICQRADATKMNMGLTSWKGKVVRKGDIVIAKNYLDNN
                     ELDSLNRLVMIFLESAELRVKNNQDLTLGFWRRNVDMLIEFNGFSVLGNGGTITHKQM
                     ESFVREQYEKFDIQRKCLKQKEADWEDLQALEKLESELTR"
BASE COUNT          709 a          490 c          403 g          677 t
ORIGIN      
        1 agccactacc tcattttgag gggagattta tacgcagtga aattttaaaa taagctaagg
       61 tttacggata atcgttcaac actattgaat tatcactgta gttatcgtta aaaaggtatg
      121 cttttatgaa ccaattcttc cctctaatct ttagcttaac tctattttcc ggcgtaactt
      181 tggcactaga aaaagcacct cgaactatgc cgacagaagg aataaataag cccctacttg
      241 gacaaattct attctttgac aatatgctgt cacacaacgg ttcacaaaac tgctccagct
      301 gccataatcc ggattatgcc tttattgata accgcactac tccggcaaaa ggggtggttt
      361 cacaaggaga tactccaacc aaattcggca aacgcaactc cccgaccatg ctttatgcga
      421 aatactcgcc tgagttccac tttgatgagc aaagtcaaga atatatcggc gggcagttct
      481 gggacggacg agccaaaaat ctcgccgagc aagccggtat gccgcctatc gacccaaatg
      541 aaatgggaat gcctaacaaa ttagaagtag ccaaaagatt atataatgtg ccgatgtatc
      601 agcgattttt agggcaattt tacggtgaaa aggtatggga aagttatgaa agcgtttatg
      661 cggcaatgga agatgcgatt gcgacattcc aacaagaaaa caaacttctc gctccatttg
      721 attcaaaata tgatctattt ttaaaaggcg aggtacaatt aactgcattg gaagaaaaag
      781 gcagagcttt atttttcaat aaagctcaaa ctaattgtag tagttgccac caattacaga
      841 gtgctgcaaa ccaccaagaa gaaaccttta gcaactatca ctattacaat gtaggcgtac
      901 ccggtaaccc agacttgata gcggttaacg gcttcgcacc agacttcgtc gatttaggct
      961 tatttgaaaa cccaaatgtt aaaggtgatg aaacacaaaa aggcaaattc aaaaccccaa
     1021 cattgcgtaa tgttgccgtg actgcacttt atatgcacaa cggtgtgttt aaagaattaa
     1081 agactgtttt gctgttttta gacagctaca ataacccgga gcgaaaaatc aaccctgaaa
     1141 ccggcacggc gtggcaacaa gcagaatttg aacccactat cgcccaccaa aaactcaaag
     1201 ccaaaccctt aagcgatgaa gaaattgatg ccctcgaagc atttttaaaa accttgacgg
     1261 atgagcggta tagaaaataa ggctacaagc ggtaaaattt tggggaaatt ttaccgcttg
     1321 cgtttaacta ccgtgttagc tcactttcta acttttccaa tgcctgcaaa tcttcccaat
     1381 ctgcctcttt ctgttttaaa catttacgct gaatatcaaa tttttcgtat tgctctctga
     1441 caaagctttc catttgttta tgcgtgattg ttccaccatt tcctaataca gaaaagccgt
     1501 taaactcaat caacatatct acatttcttc gccagaaacc taacgtaaga tcttggttgt
     1561 tcttcacacg taattcggca ctttcaagaa agatcattac caagcgattt aaagaatcca
     1621 gctcattatt atctaaatag tttttcgcaa tcacaatatc gcctttacgc acgacttttc
     1681 ctttccagct agttaatccc atattcatct tagtcgcatc agcacgttga caaatcaact
     1741 cagcggcagt gtgtttggtt attgcataaa tgagcttatt ttgcgtttct gcaaaaaaca
     1801 tttgcgtatt tttatctgtt ttgtcgtaat cgctagagag tttgaaaagc tctcgtactt
     1861 tttgatagaa acgcaattca cttgcccgaa tctcacgaat ctgttcaagt aattcatcga
     1921 aataatctaa acgaccctgc ggatttttca aacgctcgct atcaattaaa aaaccttttt
     1981 cgagataagt gcgtaatgtg ctgtttgccc attttctaaa ctgtactgct cttgggctac
     2041 gaactcgaaa ccctatcgcc agaatcattt caagtgaata atgttttact tgataattct
     2101 tgccatcttg agcagttatc aagtaatcct tgataactga attctcatct aactctttgt
     2161 cttttaagat atttttaatg tgttcggata tattttgcac agaggtgtca aaaagttctg
     2221 caagttgatt ttgtgtcagc caagcttcat tttcaaccac taacaaggct acattgctt
//
LOCUS       DANLYI010000251         2262 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.253, whole genome shotgun sequence.
ACCESSION   DANLYI010000251 DANLYI010000000
VERSION     DANLYI010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2262
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.253"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            <1..143
                     /locus_tag="PXD81_002367"
     CDS             <1..143
                     /locus_tag="PXD81_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2523 domain-containing protein"
                     /protein_id="HDL5538346.1"
                     /translation="PDLNALFGQLPESMWYFLNIFQIPMAISLMISAMISRFVRRMPF
                     IG"
     gene            153..692
                     /locus_tag="PXD81_002368"
     CDS             153..692
                     /locus_tag="PXD81_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005566788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5538347.1"
                     /translation="MAVSAYIGIPGSGKSYEVVKSVILPAVASNRRVVSNIYGLNKEK
                     IHQYLLRKNKKLMIEELGEVIYVTNEQCLESDFLPSMDEQDSFCKAGDLIIIDEVWRI
                     WGNDKDIPKNHRSFIAEHRHFADPITGVTCDLVVINQDISQIPRFIKEGLRQLTECKN
                     TLVWGCVIVIGLMFFKERI"
     gene            623..1186
                     /locus_tag="PXD81_002369"
     CDS             623..1186
                     /locus_tag="PXD81_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5538348.1"
                     /translation="MQKHVGLGLRNRYRVDVFQGKNLAKKNRTNSYQEKYDKKIFELY
                     KSYESDGAREAVTDSRQSIFSSFWVKVWIILLPLALFYCGYNLYIFLNGSESEVSHNT
                     EQAKFASDENHNDIVIHLLPTIGKPLSTKWRITGELKRDNKSFVILADNENRLRLEPS
                     SQFSFSGRMLQGEIDGGIVSYFSGESK"
     gene            1183..>2262
                     /locus_tag="PXD81_002370"
     CDS             1183..>2262
                     /locus_tag="PXD81_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspD"
                     /protein_id="HDL5538349.1"
                     /translation="MKIHQLGLLLMISSFSYAKNVDFKLEAVPLPKAISIIYDEVLQK
                     PYMLDPKLSNDSRVISFHTTEQQNFEQFIQRYFSNMNISVSEKKGVVYIGHIEPKQPK
                     VIKRSFVYSPIHRDVDYLASFLKDGTEGNQVFASGDKLVYYGTSEDIARVKSILKSID
                     TKSREVVVTGYVFEVQNIEKEGSGINLLAKLLSGKLGISVGYKQSYENFITVNAGNLD
                     AMFELFRTDQRFRVVSSPTLRVKSGSRGNFSVGSDVPVLSNVTYQDGRPIQSIEYRSS
                     GVIFDIQPTIKDKAIDLKIQQLSNFIKTDTGVNNSPTLVKRDLVTDVTVKSGDVIVLG
                     GLAENKISNGETSFSLLPKGWLSGKS"
BASE COUNT          760 a          312 c          468 g          722 t
ORIGIN      
        1 tacctgattt aaatgcgtta tttgggcaat tgcctgaaag tatgtggtat tttttaaata
       61 tttttcagat accaatggct atttctttaa tgatttcagc aatgatttca cgttttgtta
      121 gacgtatgcc atttattgga tagaggagtg ctatggctgt ttcagcgtat atagggattc
      181 ctggaagcgg caaatcgtat gaagtggtta agtcagtaat tttacctgct gttgcttcga
      241 atcgaagagt tgtttccaac atttatggat taaataaaga gaaaattcat caatatttac
      301 tgcgtaaaaa taaaaaatta atgattgaag agcttggtga agtcatatat gtaacgaatg
      361 aacaatgctt ggaatcggat tttttaccat caatggacga acaagattca ttttgcaaag
      421 ctggggactt gataattatt gatgaagttt ggcgtatttg gggaaacgac aaggatattc
      481 caaaaaatca tcgttcattt attgctgaac atcgacattt tgcagatcct ataacaggtg
      541 taacttgtga tttagtcgta ataaatcaag atatttctca aattccaaga tttattaaag
      601 aaggattgag acaacttacc gaatgcaaaa acacgttggt ttggggttgc gtaatcgtta
      661 tcgggttgat gtttttcaag gaaagaattt agctaaaaag aaccggacaa attcttatca
      721 agaaaaatat gataaaaaaa tatttgagct ttataaatct tatgaatctg atggtgcaag
      781 agaagcggtt acagatagta ggcaatctat attttcatct ttttgggtca aggtatggat
      841 tattctttta ccattggcat tattttattg tggctataac ctttacattt tcctaaatgg
      901 ttcagagagt gaagtaagcc ataatactga acaggctaaa tttgcttctg atgaaaacca
      961 taacgatata gtaattcatt tacttcctac tattggaaaa cctttatcaa ctaaatggcg
     1021 aataacaggg gaactaaaaa gggataataa atcatttgtg atcttagctg acaatgaaaa
     1081 ccgtttaaga cttgagccta gtagccagtt tagttttagc ggtcgaatgt tgcaaggaga
     1141 gatagatggt ggaatagtta gttatttttc gggagaatct aaatgaaaat ccatcaatta
     1201 gggctattgc tcatgattag ctcattttct tatgctaaga atgttgattt taaattagaa
     1261 gctgtgccgt taccgaaagc aatatcaata atatacgatg aagtgctaca aaagccatat
     1321 atgcttgatc ctaaattatc taatgattca agggttatta gctttcatac aacagagcaa
     1381 cagaattttg agcagtttat tcaacggtat ttcagcaata tgaatatcag tgtatcagag
     1441 aaaaaaggtg ttgtttatat tggacatatt gaaccaaaac agccgaaagt aataaaacgt
     1501 agttttgtgt attcgcctat tcatcgtgat gttgattatt tggctagctt cctgaaagat
     1561 ggaacagaag gaaaccaagt ttttgcaagt ggcgacaagc ttgtatatta tggtacttct
     1621 gaagatattg cacgagtgaa aagtatcttg aaatcaatcg acacaaaaag ccgtgaagta
     1681 gtggtaactg gttatgtttt tgaagtgcag aatattgaaa aagagggtag tggcataaac
     1741 ttgttagcga aattgctatc aggaaaatta ggcattagtg ttggttacaa gcaaagttat
     1801 gaaaacttta tcaccgtgaa tgcaggaaat ttagatgcaa tgtttgaatt gtttcgcaca
     1861 gatcagcgtt ttagggttgt cagcagtccg acattaagag taaaatcagg ctcaagaggt
     1921 aatttttcag taggctctga tgtccctgta ttatcgaatg taacctatca ggacggaagg
     1981 cctattcagt cgattgaata ccgttcaagc ggtgtaatat ttgatattca gccaacgata
     2041 aaagataaag cgattgattt aaaaattcaa caactatcaa atttcatcaa aacagatacc
     2101 ggtgtaaata atagcccaac gttggtaaag cgtgatctgg taacagatgt tacggttaaa
     2161 agcggtgatg ttatcgtttt aggtgggtta gctgaaaata aaatatctaa tggcgaaacc
     2221 agtttttcat tgctacctaa aggttggtta agtggaaaat ca
//
LOCUS       DANLYI010000252         2239 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.254, whole genome shotgun sequence.
ACCESSION   DANLYI010000252 DANLYI010000000
VERSION     DANLYI010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2239
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.254"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            129..377
                     /gene="rpsP"
                     /locus_tag="PXD81_002371"
     CDS             129..377
                     /gene="rpsP"
                     /locus_tag="PXD81_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HDL5538350.1"
                     /translation="MVTIRLTRGGAKKRPFYQIVVVDSRSPRDGRFIERIGFFNPLAA
                     GQAERLRLDVAKVDAWVAKGADLSDRVASLVKEARKAA"
     gene            410..937
                     /gene="rimM"
                     /locus_tag="PXD81_002372"
     CDS             410..937
                     /gene="rimM"
                     /locus_tag="PXD81_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005626427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HDL5538351.1"
                     /translation="MSEQKIEVVGKLGSTYGIRGWLRLYSSTEQTESIFDYQPWFLKI
                     KGQWQQVELESWRYHSNDLIVKLKGTEDREAAQLLTNAEIGVDLSVFPELEEGDYYWH
                     DLIGCSVVNLEGYTMGTVAEMMETGSNDVLVVRNNGKDAFGKQERLIPFLYEQVVKRV
                     DLATKTITVEWDAGF"
     gene            982..1737
                     /gene="trmD"
                     /locus_tag="PXD81_002373"
     CDS             982..1737
                     /gene="trmD"
                     /locus_tag="PXD81_002373"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HDL5538352.1"
                     /translation="MWIGIISLFPEMFKAITDFGVTGRAVKQNLLQVECWNPRDFTFD
                     KHKTVDDRPYGGGPGMLMMVQPLRDAIHAAKKAALAVEGGEAKVIYLSPQGRKLDQNG
                     VQTLASNQKLILICGRYEGVDERLIQTEVDEEWSIGDYVLTGGELPAMTLIDAVARFV
                     PGVLGKQASALEDSFAEGLLDCPHYTRPEVLDGMPVPEVLMSGHHEQIRKWRLEQSLE
                     RTWLRRPELLDSLALTDEQRVLLAKIKKQHKIS"
     gene            1764..2114
                     /gene="rplS"
                     /locus_tag="PXD81_002374"
     CDS             1764..2114
                     /gene="rplS"
                     /locus_tag="PXD81_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HDL5538353.1"
                     /translation="MSNIIKQLEQEQLKQDLPSFRPGDTLEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTLRKVSNGVGVERVFQTHSPVIDSISVKRKGAVRKAKLYYLRE
                     RSGKSARIKERLGD"
BASE COUNT          657 a          401 c          535 g          646 t
ORIGIN      
        1 gaatctccct tgtctttccg caaaaaatct gtataatttg accgcttgtt ttctcttttg
       61 aaacaagttc gttaccctca cctcgaacga gggtttattt tttactatca acatttagag
      121 gaaaggttat ggtaaccatt cgtttaactc gtggcggagc taaaaaacgc ccattttacc
      181 aaatcgtggt agtggacagc cgttcacctc gtgatggtcg ctttatcgag cgtatcggtt
      241 tcttcaaccc attagcagca ggtcaagctg agcgtttacg cttagatgtg gctaaagtag
      301 atgcttgggt tgctaaaggt gctgatttat cagaccgtgt tgcttcttta gtaaaagaag
      361 ctcgcaaagc agcttaattt ttaagccaaa attaaataag taggtgagta tgagcgaaca
      421 aaagatcgaa gttgtcggaa aattaggttc aacctatggt atccgtggtt ggttgcgtct
      481 ctattcatcg acagaacaaa ctgagagcat tttcgactac cagccgtggt ttttaaaaat
      541 taaaggtcag tggcagcagg tcgaattaga aagctggcgt tatcatagca atgatttgat
      601 tgttaaattg aaaggcactg aagaccgtga agccgcacag ttattaacca acgctgaaat
      661 tggcgtggat ttgtctgtgt ttcctgaatt ggaagaggga gattactatt ggcacgattt
      721 gatcggctgt tcggtagtaa acttagaggg ctacacaatg ggaacagttg ccgaaatgat
      781 ggaaacaggt tcaaatgatg tgcttgtggt gcgtaataac ggcaaagatg ctttcggtaa
      841 acaagaacga ttaattccgt ttttgtatga acaagtagtt aaaagagtag atctcgccac
      901 caaaaccatt acggtggagt gggacgctgg tttctaacct caggtatgcg gtgagctttg
      961 gaaaacattt aaggcgttaa aatgtggatt ggtattattt cactgtttcc tgaaatgttt
     1021 aaagcaatta ccgatttcgg ggtgacagga cgtgcagtta aacaaaatct actgcaagtt
     1081 gaatgttgga atcctcgtga ttttacattc gataaacata aaactgtaga tgatcgccct
     1141 tatggcggtg gtccgggaat gttgatgatg gtgcaacctt tgcgtgatgc gattcacgca
     1201 gcgaaaaagg cggcattagc agtagaaggc ggtgaagcta aagtgattta tctttcgccg
     1261 caaggacgta aactcgatca aaacggtgtg caaacactgg cttcaaatca gaaactgatt
     1321 ttaatttgtg gacgatatga aggcgtggac gagcgattga ttcaaacaga agttgatgaa
     1381 gaatggtcaa tcggtgatta cgttctcaca gggggggaat tacctgcaat gacattaatt
     1441 gatgcagttg caaggtttgt tcccggggta ttaggcaaac aggcttccgc gttagaggat
     1501 tctttcgcag agggattatt agattgccca cattataccc gccctgaagt attagacggt
     1561 atgcctgttc cggaagtgct gatgtctgga caccatgaac aaattcgtaa atggcggttg
     1621 gaacaatcgc tggaacgaac gtggttaaga cgccctgagc tattggatag cctagctctg
     1681 actgacgaac agcgtgtgtt gttagctaaa attaaaaaac agcacaaaat cagttaattc
     1741 taggattata gaaggttttt aaaatgagta acatcatcaa acaacttgaa caagaacagt
     1801 taaaacaaga cttacctagc ttccgtccgg gtgacacatt agaagttaag gtatgggtag
     1861 tagaaggtag taaaaaacgt ttgcaagcgt tcgaaggcgt ggttattgca attcgtaacc
     1921 gtggcttgca ctctgcattc actctacgta aagtttcaaa tggcgtaggc gttgagcgtg
     1981 tattccaaac tcactcacct gtaattgaca gcatttctgt taaacgtaaa ggtgcggtac
     2041 gtaaagctaa actttactac ttacgtgagc gttctggtaa atctgcacgt atcaaagagc
     2101 gtcttggcga ctaattgcta caaaacagtg aaatgcacaa aagccttgag gaaactcaag
     2161 gcttttgctt tttgtactta caagcagtta tttttcctca atgatttaca aatcttgctt
     2221 tcttttttgt tataatgtt
//
LOCUS       DANLYI010000253         2218 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.255, whole genome shotgun sequence.
ACCESSION   DANLYI010000253 DANLYI010000000
VERSION     DANLYI010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2218
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.255"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            34..>2218
                     /locus_tag="PXD81_002375"
     CDS             34..>2218
                     /locus_tag="PXD81_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HDL5538354.1"
                     /translation="MEKMMRKINQLIISPYFFLSILSASANSDFWKSDLNFNLTNITK
                     RVGVDNFTTPVAGEPWAGIGPNGEAAGTAPLYYSRIPAMQVTEDNKLVVMFDLRWNRA
                     YDQDRIDPGIAISSDGGHTWTKKTAWSFDRTNHPARRSMDPTLLHNPIDNSLYVMHGT
                     WSMSDQQWYGGRVPHFNSGSWAATIYKSTDGGLNWEKNTEFSKFSNLDVFSKVTKNGK
                     PTLGFLGGVGSGIVMQDGTLVFPIQTAHQNGIATTIMYSKDNGKTWEMPEIDNPVAPN
                     QSSLENMVFELEPGKLVMTGRGNSRWAYSSTDMGKTWELFTPTNGLLPTSSQPTQGSS
                     IYVTLPNGRKVLLISKPDGKNDGWKRGDITLWMLDAKDPSHKHKVHIIRPGSGNAAGA
                     GYSSLAYKEGNLFVAFENDGDISVKNLTEHIAEMEAKAIEWGLPDELTPALDKINALP
                     YLNKAQKETLVEKMRRANDTAIAQSFVINKEMYNLKNNSDELDKLAKNITKALPSQQN
                     IYQRALAYVNKVTNTADTTYLNYNGIMDTYANLYESYLALNTKLDFTRYIQKARKFNE
                     YNTDILYRSFDNFFAHYDTGVKHNNLSLGLNTKLSENLRGGLFFEYSNKNRNSVQIGA
                     RAKYEMDNHQLSGFLRYRGVKHKDMLARNNSVDGYLNYAYRIQLDDKLSISPSVGAYV
                     SRSSRSLIDEDLAINSRTVYASDVGLNIHYKLNDIDAYIRPSIGFV"
BASE COUNT          718 a          397 c          433 g          670 t
ORIGIN      
        1 gctaaaaatt agtctttgca ctttatcccc ctaatggaga aaatgatgag aaaaatcaat
       61 cagttaatca tatccccata ctttttccta agtattttat ctgcttccgc taactctgat
      121 ttttggaaaa gcgaccttaa ttttaatctc acaaatatta ccaagcgtgt cggagtcgat
      181 aactttacca ctccagtagc tggcgagcct tgggctggta ttggtccaaa tggtgaagct
      241 gcaggtactg cgccattata ttatagccgt attccagcta tgcaagttac cgaagataac
      301 aaattagttg ttatgtttga tttacgctgg aatcgtgcgt atgaccaaga tcgtattgat
      361 cctggtattg cgatttcttc cgatggtggc catacttgga cgaagaaaac cgcttggtct
      421 tttgatagaa ctaatcaccc agcacgccgt tcaatggatc caacgctatt acataatcct
      481 attgataata gtttatatgt aatgcacgga acttggtcta tgtcggatca acaatggtat
      541 ggtgggcgtg ttcctcactt taatagtggc agctgggcag ctaccattta taaatctact
      601 gatggtggtt taaattggga gaaaaatact gagtttagta aattcagcaa tttagacgta
      661 ttttctaaag tcaccaaaaa tggaaaacct accttaggct ttttaggcgg ggtcggatct
      721 ggaattgtaa tgcaagatgg tacgttggtt ttcccaattc aaaccgcaca tcaaaatggt
      781 attgcgacca ctattatgta ttctaaggat aatgggaaaa cttgggaaat gccagaaatt
      841 gacaacccag ttgcaccgaa ccaaagttct ttagaaaata tggtgtttga actcgaaccg
      901 ggtaagctag tgatgactgg acgaggaaac agtcgctggg catactcttc tactgatatg
      961 ggtaagacct gggaattatt tactccaact aatggattat taccaactag ctcacaacca
     1021 actcaaggct cttctatcta tgtaacacta ccaaatggtc gaaaagtgct actaatctct
     1081 aaaccagatg gaaagaatga tgggtggaaa aggggtgata ttacactttg gatgctagat
     1141 gcaaaagatc catcgcacaa acacaaggtg catattattc gtccaggttc aggtaatgcc
     1201 gctggtgctg gttattcctc tttagcctat aaagaaggga atttatttgt tgcttttgaa
     1261 aacgatggtg atatttcagt aaaaaattta actgaacata ttgctgaaat ggaagcaaaa
     1321 gctatcgaat ggggattacc agatgaactt actcctgctt tggataaaat caatgcactg
     1381 ccttatctca ataaagctca aaaagagacg ctagtcgaaa aaatgcgtcg tgctaatgat
     1441 actgcgattg cacaatcttt tgtgattaac aaagaaatgt ataatctgaa aaacaatagt
     1501 gatgagctgg ataaattggc aaaaaatatt actaaagcac tgccgtctca acagaatatt
     1561 tatcaaagag ccttagccta tgtgaataaa gtcaccaata cagcggatac cacttattta
     1621 aattataatg gtattatgga tacctatgct aatttatatg aaagctattt ggctttaaat
     1681 actaaattag acttcactcg ctacatccaa aaagcgagaa agttcaatga atataacact
     1741 gatattttat atcgtagctt tgataatttc tttgcccatt acgacacagg tgtaaaacac
     1801 aataatctat ccttaggctt gaatacaaaa ttaagtgaga atttacgtgg tggcttattc
     1861 tttgaataca gtaataaaaa tcgtaatagc gtacaaattg gtgcaagagc taaatatgaa
     1921 atggataatc accaactttc tggcttctta cgctatcgtg gcgtaaaaca taaagatatg
     1981 ctagcacgta ataacagtgt tgatggctac ctaaattatg cttatcgtat tcaattagat
     2041 gataaattaa gcattagccc atcagttggt gcttacgttt cacgttcttc tcgctcatta
     2101 atagatgaag atcttgcgat taatagccgc acggtctatg caagtgatgt aggattgaat
     2161 atccattata agctaaatga tattgatgct tatattcgtc caagtatagg ctttgtca
//
LOCUS       DANLYI010000254         2218 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.256, whole genome shotgun sequence.
ACCESSION   DANLYI010000254 DANLYI010000000
VERSION     DANLYI010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2218
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.256"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            80..1675
                     /locus_tag="PXD81_002376"
     CDS             80..1675
                     /locus_tag="PXD81_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HDL5538355.1"
                     /translation="MQRHEQLAQVQQTQKWDFIIIGGGASGLGIAVDAASRGYKTLLL
                     ESHDFAKGTSSRSTKLVHGGVRYLANGDVALVREALRERGRLAKNAAHLFKNQSFIIP
                     NYKFKDCVMYRVGLGLYDMLSGNLSLGKTENISKTEAKRRLPTLNDTGLRTGTVYQDG
                     QFDDSRLAVNMAQTVVEHGGTVLNHAKVTSLHKTPQGKIDGVTFYDELNQQEHTVFGT
                     AVINATGVFMNDILSMDHGSTKKFVVPSQGIHLVLDKSFLPSNDALMIPKTSDGRVLF
                     AVPWHEVLVVGTTDTLIENPSYEPIPLEQEVNFILETAAQYLTKKPTRDDVKAVFAGQ
                     RPLAAPEKEGQSTKEVSRSHKVLTDATSGLITITGGKWTTYRQMAEDTVEEALKVHSE
                     LGNKPCVTVNLPIHGNIPAAQVNLQDHLYVYGSDIPQLRALMTETPEYAQKIHPRLDY
                     TVAEVIWAVRHEMAQTVEDVLARRVRLLFTDARAAIDSAAKVAAIMAKELGKDEMWQQ
                     QQVIQFLDVAKHYLLVDYQPQPI"
     gene            complement(1762..2061)
                     /locus_tag="PXD81_002377"
     CDS             complement(1762..2061)
                     /locus_tag="PXD81_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5538356.1"
                     /translation="MENIKMEELKLTEKELLVLKGNALRMRFKAETNKTKNEIMQPFK
                     QVKQTGLALVNSPAVKTIALDFLVKRLLSMKGISFSVLGLTAFFLLQQKHKRDDS"
     gene            complement(2048..>2218)
                     /locus_tag="PXD81_002378"
     CDS             complement(2048..>2218)
                     /locus_tag="PXD81_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HDL5538357.1"
                     /translation="FFSIAIIMFIAVTICGMMILSALKKQRHFMQSTLTELKLDIAAF
                     KSALTLKQNGEH"
BASE COUNT          720 a          418 c          448 g          632 t
ORIGIN      
        1 taatttccta aaaaggaatt actcagaggt tttactaagt aatgattaaa tgttttaatt
       61 cgagcaaaag gagcaaaaaa tgcaaagaca tgaacagcta gctcaagtac aacaaacgca
      121 aaaatgggat tttattatta tcggtggtgg cgcaagtggt ttggggattg cggtagatgc
      181 agcttcccgt ggctataaaa ccttattatt agaaagccat gactttgcga aaggtacatc
      241 gagccgaagc acaaaattag tccacggtgg cgttcgctat cttgcaaatg gcgatgttgc
      301 actcgtgaga gaagcattgc gtgaacgagg tcgtttagct aaaaatgcag ctcatttatt
      361 taaaaaccaa agctttatta ttccaaacta caaattcaaa gactgtgtaa tgtatcgtgt
      421 tggtttaggc ttatacgata tgctttcagg caacctgagt ttaggaaaga ctgaaaacat
      481 cagtaaaact gaagcgaaaa gacgtttacc aaccttaaat gatactggtt tacgtacagg
      541 cactgtttat caagatgggc aatttgatga ctcacgtctt gcagtgaata tggcacaaac
      601 ggtagtagag cacggcggta cagtactaaa tcacgccaaa gtgacttcac tgcacaaaac
      661 gccacaaggc aaaattgatg gggtgacttt ctacgatgaa ctcaatcaac aagaacatac
      721 tgttttcggc actgcggtga ttaacgcaac cggcgtattt atgaatgata ttttaagtat
      781 ggatcacggt agcacgaaaa aatttgtcgt accaagccaa gggattcact tagtattaga
      841 taaatcattc ttaccaagta atgatgcgtt aatgattcca aaaacctctg acggacgagt
      901 gttatttgct gtaccttggc acgaagtgtt agtcgtaggg acaacagata ctctgattga
      961 aaatccttct tatgagccaa ttccattgga acaagaggtg aactttattt tagagacagc
     1021 cgcacaatat ctgacaaaaa aaccaactcg tgatgatgta aaagcagtct ttgccggtca
     1081 acgtccgctt gcggcaccgg aaaaagaagg tcaaagcact aaagaagtat ctcgtagtca
     1141 caaagttctc actgatgcaa ccagcggttt gattacgatt acaggtggta aatggacaac
     1201 ttatcgtcaa atggcagaag ataccgtaga agaagcgtta aaagtccact ctgaactagg
     1261 caataaacct tgtgtgaccg tcaacttacc gattcacggt aatattccgg cagcacaagt
     1321 gaatctacaa gatcatctat atgtatatgg ctcagatatt ccacagttac gcgccttaat
     1381 gactgagacc ccggaatatg cacaaaaaat ccatccgaga ctagattata ccgttgcaga
     1441 agtgatctgg gcagttcgcc acgaaatggc acaaacggtt gaggatgtat tagctcgccg
     1501 tgttcgtttg ctcttcactg atgcgagagc ggcgattgat agtgcagcaa aagtggctgc
     1561 tattatggcg aaagaattag gtaaagatga aatgtggcaa caacagcaag ttatccagtt
     1621 ccttgatgtt gcgaagcact atttattggt agattatcag ccacagccaa tctaatacgc
     1681 taaaagcaag cggttaaaat cgataaaaat attgcaatta cgttatttgc aactttttaa
     1741 accattttaa ccgcttgtat attagctatc atctctttta tgtttctgtt gtaataaaaa
     1801 gaatgctgtt aaccccaata cagaaaagct aattcctttc atactcaata atctttttac
     1861 caaaaaatct aatgcaatgg ttttaactgc cggtgaattg accagtgcaa gccctgtttg
     1921 tttcacttgt ttaaaaggct gcataatctc atttttggtt ttattggttt ccgctttaaa
     1981 tcgcattcta agtgcattac cttttagaac tagaagttcc ttttcggtta atttaagctc
     2041 ttccatttta atgttctcca ttctgtttca aagttaaagc actcttaaat gcggcaatat
     2101 ctaattttag ctcggttaat gtactttgca taaaatggcg ttgttttttc aaggcagaaa
     2161 gtatcatcat tccgcaaata gtcacagcaa taaacataat aatggcaata gaaaagaa
//
LOCUS       DANLYI010000255         2207 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.257, whole genome shotgun sequence.
ACCESSION   DANLYI010000255 DANLYI010000000
VERSION     DANLYI010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2207
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.257"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            90..2021
                     /locus_tag="PXD81_002379"
     CDS             90..2021
                     /locus_tag="PXD81_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL5538358.1"
                     /translation="MIFFTDLILKRGHSVLIDNSSVTIHTGQKVGLVGKNGCGKSSLF
                     ALLKNELQPEGGDVSLPKNWAISWVNQETPALAISALDYVIQGDREYTHLMAELDIAN
                     EKNDGNRIAIIHTQLDTIDAWTIQSRAATLLNGLGFSTEQLQLPVKSFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLERWLSNYRGTLLLISHDRDFLDPIIDRVIH
                     IEHQKLNEYTGNYTSFEIQRATKIAQQNAAYQQQQRKVAHLQSFIDRFKAKATKAKQA
                     QSRIKALEKMELIAPAYADSPFEFEFRPPLALPSPLLMMEKVSAGYAEKTVLESVKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELPPQNGSIQLAKGVQLGYFAQHQVDTLRFDE
                     SPLWHLQKLAPEKTEQELRNYLGGFDFKGDKVVQAVKSFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLEMRQALVDALTYYEGSLVVVSHDRHLLRSTVNEFYLVHDRKVEKF
                     KGDLDDYQKWLNEQNALANEPKSVLAKDSANSTACSSTNENSAAQRKEQKRLEAERRQ
                     QTAPLRKKITQLEKELEKATEKLNKLEAMLTESDIYEAENKAKLTETLAKQLEAKRLV
                     EEIEMQWLEAQEEFETMLR"
BASE COUNT          694 a          471 c          478 g          564 t
ORIGIN      
        1 actgaatttc ttattttagt tcacaaagca atggctttgc ttttttatgc taaaatacgc
       61 aaaattttta acaaaataga ccgcttgcaa tgatcttttt tacagactta attttaaaac
      121 gagggcattc cgttctcatt gataacagct ccgttaccat tcacaccggg cagaaagtcg
      181 gtttggtggg aaaaaacggc tgtggcaaat cctctctttt tgccttactg aaaaatgaat
      241 tacaacctga aggtggcgat gtatcgttgc cgaaaaactg ggcgatctcg tgggtaaatc
      301 aagaaacgcc tgcgttagct atttcagcgt tagactatgt gattcaagga gatcgggaat
      361 atacccatct tatggctgag ctagatatcg caaatgaaaa gaatgacggc aaccgcattg
      421 cgattatcca tactcaactt gataccattg atgcttggac aattcaatcc cgtgccgcta
      481 ccctgcttaa cggcttgggg tttagcaccg aacaactgca actgccggtg aaatccttct
      541 ccggtggctg gcgtatgcgg ttaaatttgg cacaagctct gatttgtcgt tccgatctgt
      601 tattgcttga cgagccaacc aaccacttag acttagatgc ggttatttgg ttggagcgtt
      661 ggctgagtaa ctatcgtggc actttattgt taatttcgca cgaccgtgat tttctcgatc
      721 cgattatcga tcgggtgatt catattgaac atcaaaagct caatgaatac accggcaatt
      781 acacctcgtt tgagattcaa cgtgcgacca aaatcgccca gcagaatgcc gcttatcagc
      841 aacagcaacg taaagttgct catttgcaga gttttatcga tcgctttaaa gccaaagcga
      901 ctaaagcgaa acaggcacaa agccgaatta aagcgttgga aaaaatggag cttattgccc
      961 cagcctatgc tgattcgccg tttgaatttg aatttcgccc accgcttgca ctgcctagcc
     1021 cgttattaat gatggaaaaa gtcagtgccg gctatgcgga aaaaacggtg ttggaatcgg
     1081 tcaaactgaa tttggttccc ggctctcgta ttggtttact cgggagaaac ggtgcaggga
     1141 aatccacgct gattaaatta ttggcgggcg aacttccgcc ccaaaacggc tcaattcaac
     1201 tggcaaaagg cgtgcaactc ggctatttcg cccaacatca ggtggataca ctacgttttg
     1261 atgaaagccc actttggcat ttacaaaaac tcgcaccgga aaaaaccgag caggaattac
     1321 gcaattattt aggcggtttc gattttaaag gcgataaagt ggtacaagct gtgaaatcat
     1381 tctccggcgg agaaaaggcc cgtttagtgc ttgccttaat tgtatggcaa cgcccgaatt
     1441 tactgctatt agatgagcca accaaccatt tagatttaga aatgcgacaa gccctggttg
     1501 atgcactgac ctattacgaa ggctcgctag tggtggtttc gcacgaccgc catttattgc
     1561 gtagcaccgt gaatgaattt tatttagtgc acgaccgcaa agtggaaaaa tttaaaggtg
     1621 atttagacga ttaccaaaaa tggctcaatg agcaaaacgc cttagccaac gagccgaaat
     1681 ccgtacttgc aaaagattcg gcaaattcga ccgcttgctc tagcaccaat gaaaatagtg
     1741 cagctcaacg caaagagcaa aaacgcttag aggcagagcg ccgccaacaa accgccccat
     1801 tgcggaaaaa aattactcaa ctggaaaaag agttagaaaa agcaaccgaa aaattaaata
     1861 aactcgaagc aatgcttacc gaaagcgaca tttatgaagc cgaaaataaa gcaaaactaa
     1921 ctgaaacact tgcgaaacag ttagaggcaa aaaggctggt tgaagaaatt gaaatgcaat
     1981 ggttggaggc tcaggaggag tttgagacca tgttacgcta gggttctaca agatagcgca
     2041 agcgtccacg cttgtgccat ttaagggaac tatacacctc aaaaaaacaa tgatcattaa
     2101 gctagctttt ttataaattt aaaaacctat agattaaata aataatgata ttaatagtaa
     2161 aatcacgagt gcaactacac tgacgataat aaaatcaggc atactta
//
LOCUS       DANLYI010000256         2185 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.258, whole genome shotgun sequence.
ACCESSION   DANLYI010000256 DANLYI010000000
VERSION     DANLYI010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2185
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.258"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            <1..682
                     /gene="fdhD"
                     /locus_tag="PXD81_002380"
     CDS             <1..682
                     /gene="fdhD"
                     /locus_tag="PXD81_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HDL5538359.1"
                     /translation="VMMATPANLEDFALGFSLAEGILNAPSELYGLDVIEQENGVEIQ
                     LEISSRQFVALKDKRRSMAGRTGCGICGVEQLEQVNQSCKIVKKNNRLQNVNPLILED
                     CLLQLENAQFLSKETGASHAAAFFTPDGKLLAIREDVGRHVALDKLIGWYAKADFPIG
                     FVFVTSRASFEMVQKCLAVGIEMLCAISATTEMAVNMARENQFTLLAFARKGKATIYA
                     GEERLALN"
     gene            685..1614
                     /locus_tag="PXD81_002381"
     CDS             685..1614
                     /locus_tag="PXD81_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005601223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HDL5538360.1"
                     /translation="MEIALLLADKILQLTLMVLLGYLIVKTKLLKSEHSYPISVIGLY
                     IISPAMLITAFQVDYTTEILNGLYLAFLISVLLNGLLILLGWLLKRALKLDNIEQATA
                     IYSNSGNLIIPIVASLFGNEWVIYSTAFIVVQNVLFWSHLRLLICGKGNVPFRKMIFN
                     INIIAIVIGMMLFLLHIKLPATLANTLSTLGHMLGPLAMLVAGMLIAAIPFKEIITDK
                     RIYLVAFLRLIFIPLILLAVIKLGGFASWIENGKTVVMITFLATISPSAATATQMAVA
                     YGQDAKKASAIYGVTTLLCVFTMPLIIWLYQFL"
     gene            complement(1706..>2185)
                     /locus_tag="PXD81_002382"
     CDS             complement(1706..>2185)
                     /locus_tag="PXD81_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HDL5538361.1"
                     /translation="IKIELNFEKAPITAKNFEDYAKEGFYNGTIFHRVIDGFMIQGGG
                     MTAGMAEKPTKDPIQNEANNGLSNKRGTIAMARTSDPHSASSQFFINVADNTFLDYRS
                     KEMFGRSVVQDWGYAVFGEVVEGMDVVDKIKGVKTGNKGFHQDVPVEDVVIESVSVA"
BASE COUNT          597 a          448 c          468 g          672 t
ORIGIN      
        1 tgtaatgatg gcaacaccgg ctaatttgga ggacttcgct ttaggattct cattggcgga
       61 agggatttta aatgccccat cggaattata tggtttagat gtaattgaac aagaaaatgg
      121 agtagaaatt cagcttgaaa tatcgagccg ccaatttgtt gcattaaaag ataaacgccg
      181 ctcaatggca ggcagaacag gttgcggtat ttgcggtgta gagcaattag agcaggttaa
      241 tcaaagttgc aaaattgtta agaaaaataa ccgcttgcag aacgtcaatc cactgatttt
      301 agaggattgt ttattacagc tagaaaatgc tcaattctta tcgaaagaaa ccggtgcaag
      361 ccacgccgct gcatttttta ccccggatgg aaaactgctt gccattcgtg aagatgtggg
      421 cagacacgtg gcgttggata aattaatcgg ttggtatgct aaagctgatt ttccgattgg
      481 atttgtgttt gtaaccagtc gtgccagctt tgaaatggtg cagaaatgct tagccgttgg
      541 cattgaaatg ttgtgtgcca tctcagcgac cactgaaatg gcagtgaata tggctcgtga
      601 aaatcaattt accttacttg cttttgcccg caaaggcaaa gcgaccattt atgcaggaga
      661 agagagactc gctcttaatt gataatggaa atagcccttt tattagccga taaaattcta
      721 caattaaccc tgatggtgct gctcggctac ctcatcgtta aaactaaatt actcaaatcg
      781 gagcacagtt atccgatttc ggttatcgga ctttacatta tcagcccggc aatgctgatt
      841 actgcttttc aagtagatta caccaccgaa atactaaacg gcttgtatct ggcattttta
      901 atctccgtct tattaaacgg cttgttaatt ctgcttggtt ggctgctcaa aagagcgttg
      961 aaattagaca acatagaaca agccaccgct atttattcca actccggcaa cttaattatt
     1021 ccgattgttg cctccctgtt cggcaatgaa tgggtgattt attcaacggc ttttattgtg
     1081 gtgcaaaatg tgttgttttg gtctcactta cggctgctga tttgtggcaa aggcaatgtg
     1141 ccgtttagaa agatgatctt taatatcaac attattgcga ttgtgattgg tatgatgttg
     1201 tttttgctac atatcaaact gcccgctacg cttgcaaaca cgctctcaac tttagggcat
     1261 atgttagggc ctttagcaat gcttgtggca gggatgttaa ttgcagcaat tccatttaaa
     1321 gagattatta ccgataaacg catttacttg gtggcttttc tccgcttgat ttttattccg
     1381 ctgattttgt tagcggtgat taaactcggc ggttttgcga gctggataga gaatggaaaa
     1441 acggtggtaa tgattacatt cttagccacc atcagccctt ctgctgcaac ggcgacccaa
     1501 atggcggtgg cttacgggca agatgccaaa aaagccagtg cgatttatgg cgtaaccact
     1561 ttactttgtg tgtttacaat gccgctgatt atttggctgt atcaatttct ataaaaacaa
     1621 gcagtcgatt tctgctactt ttttgcaacg ctgagaaatg caaaaaattg tgaaaaatca
     1681 accgcttgta ggctaatttg cggaattagg caacgcttac agactcaatc accacatctt
     1741 caaccggcac gtcttggtgg aagcctttat tgccggtttt tacgcctttg attttatcga
     1801 ccacgtccat accttcaacc acttcgccaa atactgcata gccccaatct tgcactacac
     1861 ttctgccgaa catctctttt gagcgataat ctaagaaagt attatccgcc acattaatga
     1921 aaaactgtga cgaagcagaa tgcggatcag aagtgcgagc cattgcgatt gtgccacgct
     1981 tgttgcttaa accgttgttc gcttcgtttt gaatcggatc ttttgtcggt ttttcagcca
     2041 tacctgccgt cattccgcca ccttgaatca taaagccgtc aatcacacgg tggaaaatcg
     2101 tgccgttata aaacccttct ttagcgtaat cttcaaagtt tttagcggta atcggggctt
     2161 tttcaaagtt taattcgatt ttaat
//
LOCUS       DANLYI010000257         2179 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.259, whole genome shotgun sequence.
ACCESSION   DANLYI010000257 DANLYI010000000
VERSION     DANLYI010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2179)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2179)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2179
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.259"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            316..>2179
                     /locus_tag="PXD81_002383"
     CDS             316..>2179
                     /locus_tag="PXD81_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraI domain-containing protein"
                     /protein_id="HDL5538362.1"
                     /translation="MHSILKKILGKSKSSSQSSVKSDNVFAIEQDSEGWLYPKNASQL
                     LNTELRQKYLGLLWQQVSMNRQMFEVLYQQPIERYAEMVQLLPASESHHHSHIGGMLD
                     HGLEVIAFSAKVRQNYILPPNVAPEEQSKQRDAWTAAAIYLALVHDIGKVVVDIEITL
                     KDGSRWFAWNGQPPQPYKFKYIKGRDYHLHPVLGGFLANQLIPKSAFDWLGQYPEVIS
                     SLMYAMSGHNDKAGLLSEIVQKADQHSVTLAIGGDVNKLVQRPVNSLPKQIVLALRYL
                     LEQKIKLNTPKGPADGWFTEDGLWLVSKTAADNIRTYLLGQGISVPKDNPKLFDEMQS
                     LGIVEESPSGGAIWSCRIQADSGWTPATTFTLLRVKPEVAWENINDRPAYFAGKVKVI
                     EQITEEVNAEVQPPLVEETTKISLLPSKQSEAIIPSEETEISKAITSSDNATSAEVII
                     NNENDLTSFVLDMFAPTETIEQNISVISSNKEQQIVMTTLTEQEKVPPKKVKLEKSKK
                     KNLQLTTLEPWNDMINPITGKEFVNWLKEGIATNKFAINKPSAKLHIVDGNLFLVTPV
                     IFEMYLQECGQPYDKETINNLQYDFQGLGLHSLKKTMTNGKPDTINFWKCDVIGP"
BASE COUNT          705 a          418 c          449 g          607 t
ORIGIN      
        1 atatcaaagt tatctggtta ctatcattcg tgtattgtaa tctcgcaatt caacatcact
       61 aataaaaaat tctccaacat attcagagag ttttttctac tgataccaac taaattttgg
      121 ttcaatactc gaagttaaat ttggttcaga gaaaaaagcc tctaaatggc aaaagggtaa
      181 ggggccctaa cgggcaaggg taatccccta agggggataa gagtaacctt tccaagggga
      241 aaggtcttac ccaaaagggc aaagggctaa acggctttct ttcaacgcaa acatcatctt
      301 tttcggtcta tttcaatgca ctcaattctc aaaaaaattc ttggcaaaag taaatcctcg
      361 tctcaaagtt cggttaaatc tgacaatgtt ttcgctattg agcaggatag tgagggctgg
      421 ttatatccga aaaatgcctc ccaactactt aatacggaat tacgccaaaa atatctgggt
      481 ctactttggc aacaggtatc aatgaaccgt caaatgtttg aggtacttta tcagcagcca
      541 atagaacgtt acgctgagat ggttcaacta ttaccagctt ctgaatcaca tcatcattca
      601 catatcggcg gaatgctcga tcacggtttg gaagttattg ctttttcagc caaagtccgt
      661 caaaactata tattgccacc gaatgtcgca cctgaagaac aatctaagca acgagacgct
      721 tggactgccg ctgcaattta tcttgccttg gttcatgata ttggcaaagt tgtggttgat
      781 attgaaatca ctcttaaaga cggctctcgt tggtttgctt ggaatggaca gccacctcaa
      841 ccttataaat tcaaatacat caaaggtcga gactaccatc ttcatccggt gctaggagga
      901 ttccttgcaa atcagctcat tcccaagtct gcgtttgatt ggctgggaca gtatccagaa
      961 gtcatttctt cactgatgta tgcaatgtct gggcataacg ataaagccgg gcttttatct
     1021 gagatcgtgc agaaagccga tcagcatagt gttacgctag caatcggtgg cgatgtgaat
     1081 aagctggtac agcgaccagt taattcattg ccgaagcaaa ttgtgctggc tctacgttat
     1141 ttactggagc agaaaatcaa attaaatacg ccaaaaggac cagcagacgg ctggtttacc
     1201 gaagatgggc tgtggctggt aagtaaaact gctgccgata atatccgaac ttatttactg
     1261 ggacagggga tttccgtgcc taaagataat ccaaaactgt ttgatgaaat gcaatctctt
     1321 ggtattgtgg aagagtctcc ttccggcggt gcaatttgga gctgccgtat ccaagccgat
     1381 tccggttgga caccggcgac aacatttacg ctattacgag ttaaaccgga ggttgcttgg
     1441 gagaatatca acgatagacc ggcgtatttt gccggaaaag tcaaagtgat tgaacagata
     1501 actgaagagg tcaatgcaga ggttcagcct ccattagttg aagaaaccac taaaatttct
     1561 ctattaccga gcaagcaatc ggaggctatt attccgtctg aggaaacgga aatatcgaag
     1621 gcaattacaa gttcagacaa tgcaacatct gcggaagtaa tcataaataa tgaaaatgat
     1681 ttaacttctt ttgtattgga tatgtttgct cctacagaga caattgagca aaatatttct
     1741 gtcatttctt caaataaaga gcagcaaatc gtaatgacaa cacttacgga acaagaaaaa
     1801 gttccgccga aaaaagttaa attagagaaa agtaagaaaa aaaatcttca attaaccact
     1861 ctagaaccat ggaatgatat gattaatcca attactggaa aagaattcgt aaattggttg
     1921 aaggagggga tagcaaccaa taaatttgca attaacaaac cttctgcaaa attacatata
     1981 gtagatggta atttattttt agtcacacca gttatttttg aaatgtattt gcaagaatgt
     2041 ggccaacctt atgataagga aactataaac aatcttcagt atgatttcca ggggcttggt
     2101 cttcattctc ttaaaaaaac catgactaat ggtaagccag atacaataaa tttttggaag
     2161 tgtgacgtga ttggtccta
//
LOCUS       DANLYI010000258         2141 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.260, whole genome shotgun sequence.
ACCESSION   DANLYI010000258 DANLYI010000000
VERSION     DANLYI010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2141
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.260"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            17..>2141
                     /locus_tag="PXD81_002384"
     CDS             17..>2141
                     /locus_tag="PXD81_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent
                     hemoglobin/transferrin/lactoferrin family receptor"
                     /protein_id="HDL5538363.1"
                     /translation="MQIKQTVLAIAITPFISQLSLAQETKISSIELDTIEVNEQQENL
                     LKQQAVMSNEDLVRYSTDMGVPQTGRFGPQGFNIRGVEGNQVAVTVDGISQAASNNYA
                     TFSRYSYYNKSRPEIDTELMSDVTIEKGSSTKVGNGALGGSVNYKTKTVSDILMKGRQ
                     FGGMLKYTYNGQNKENIETAGIAADGQYADALVMYSHRQGHETQTTSKGPDIMGSGRG
                     LPNPYKNNQYSYLAKLGLKWNDENRLELKTSKQRSKNAGQELSYSTVTLRDFLDVQEI
                     ETYGVTYLYTPKNSILENIKVAVTKQDTEISGINDQITIARGTKTDNTKRAFNTKNVE
                     GSLGVTFKPVDLGNTEHKLSTELGYGVSDFSFDMQTYTAGITPTDQNMQSPAKTKSFH
                     ILMGDSILLTQNLSAHLNARYEQSKLHSDDNSLSDKTFNNWAGDIGLNYQLSPVWQIG
                     YKLSRGFRTPTASEMFFNREVQGRGASQTFLANTNLKPETSLGNEISLVGNGSLGSLS
                     VAAYHTHYRNLIDLATIGNNIYQSQNVNRAKIKGVDINGTLDLNTAWNAIPQGFELNG
                     GFGYARGKRNDGTDLLTVQPLKALFGIGYRAVNNDWAIHLNGTYTQGKKAKYAQQYAI
                     LANTGYLSYDKSLTTYPYLSNSATVFDLVAHKKFGERAIVKVGVYNLFNKKYWTWDSL
                     RTIGITSPGVANSITGEGLNRYLAPE"
BASE COUNT          727 a          387 c          432 g          595 t
ORIGIN      
        1 ctagaggatc taaaatatgc aaataaagca aactgtatta gcaatagcga ttactccctt
       61 tatttctcaa ctctccttag ctcaagaaac aaagattagt agcattgaac tagatactat
      121 tgaagtaaat gagcagcagg agaacctact taaacagcaa gcggtgatga gcaatgaaga
      181 tttagtacgt tatagtactg atatgggcgt tccgcaaaca ggccgctttg gcccacaagg
      241 gtttaatatc cgtggtgttg aaggtaatca agtggcagta acagttgatg gcatatcgca
      301 agcagcatca aataactatg ccacctttag tcgctatagt tactacaata aatctcgccc
      361 tgaaatcgat actgaattaa tgagcgatgt aaccattgaa aaaggctcaa gcacaaaagt
      421 aggcaatggg gcattaggcg gctctgtgaa ctataaaact aaaaccgtaa gtgatatttt
      481 aatgaaaggt cgccaatttg gtgggatgtt gaaatatacc tataacggtc aaaataaaga
      541 gaatattgag acggcaggta ttgctgcgga tggtcaatat gcggatgctt tggttatgta
      601 tagccaccgt caaggacacg aaacacaaac aacgtctaaa ggtccggata ttatgggaag
      661 tggtcgtggt ttaccaaatc cttacaaaaa taatcaatac agctacttgg caaaattagg
      721 gctaaaatgg aatgatgaaa atcgccttga attaaaaact tctaagcaac gcagtaaaaa
      781 cgccggtcaa gagctttctt attctaccgt cacattacgt gattttttgg atgtgcagga
      841 aattgaaact tatggcgtta cttatttata cactccaaaa aacagtattc ttgaaaatat
      901 taaagttgcg gtaaccaaac aggatactga aatttcaggt attaacgatc aaatcactat
      961 tgcccgtggt acaaaaacag ataataccaa acgggctttt aatactaaaa atgttgaagg
     1021 tagcttaggt gttacattca agcctgttga tctaggtaat accgaacata aattgtcaac
     1081 ggaattaggc tatggtgtat ctgatttcag ttttgatatg caaacctata cagcaggtat
     1141 tacgccaacc gatcaaaata tgcagtctcc ggcaaaaacc aagtctttcc atattttgat
     1201 gggagactct attttactga cacaaaatct atctgctcat ttaaatgctc gatatgagca
     1261 gtcgaaatta cattctgatg ataatagttt gtcagataaa acctttaaca attgggcggg
     1321 agatattggt ttaaattatc agctctctcc tgtatggcaa attggctata aactcagccg
     1381 tggttttaga acacctaccg caagtgaaat gttctttaac cgtgaagttc aagggcgtgg
     1441 agcaagccaa actttcttag caaatactaa cttaaaaccg gaaacaagct taggtaatga
     1501 aatttcactg gtaggtaatg gttcattagg tagcttgagc gtagccgctt accataccca
     1561 ctatagaaat ctgattgatc ttgccactat tggtaacaat atttaccaat cgcaaaatgt
     1621 taaccgagct aaaatcaaag gtgttgatat taacggaact ttagacttaa acacagcgtg
     1681 gaatgcaatt ccacaaggct ttgagcttaa tggtggattt ggttatgctc gtggcaaacg
     1741 taatgatggt accgacttac tcacggttca accgttaaaa gcattgttcg gcataggtta
     1801 tcgagctgtg aacaatgact gggcgataca cctaaacggc acatacacac aaggcaaaaa
     1861 agcaaaatat gcacaacagt atgctatttt agccaatact gggtatttaa gttacgataa
     1921 atcattaacc acttacccat acttaagtaa ttcagccacc gtatttgact tagttgcaca
     1981 caaaaagttt ggagaaagag cgattgttaa agttggggtt tataatctct ttaataaaaa
     2041 atattggact tgggatagtt taagaactat tggtataact agtcctggag ttgccaacag
     2101 tataactggc gaaggcttaa atcgttacct tgctccagag c
//
LOCUS       DANLYI010000259         2119 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.261, whole genome shotgun sequence.
ACCESSION   DANLYI010000259 DANLYI010000000
VERSION     DANLYI010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2119)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2119)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2119
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.261"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            <1..353
                     /locus_tag="PXD81_002385"
     CDS             <1..353
                     /locus_tag="PXD81_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014992182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HDL5538364.1"
                     /translation="LQGVDSDFATFIRTVVIIIALIAFLTYSGKWQPLDSLSSKNWLF
                     LILSGLATGLSWSAYFKALQMGNASQVAPIDKFSLVLVTLFAVIFLGERPSGQDWLGI
                     GLVAGGVLLLAFKR"
     gene            359..1081
                     /locus_tag="PXD81_002386"
     CDS             359..1081
                     /locus_tag="PXD81_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HDL5538365.1"
                     /translation="MQNFANIEIAFIHRKAPLPSDFSYPEMPGGLTEKQVQKWQSRRV
                     AFFLLTELFKKHHLDLDLLADMQRSPSGRTFVDSEQIDFNISHSGEWIAVIFSYSFAK
                     LAVGIDIEHPQKIRRYHDLICHYGNAEEQNVLLANHSPILSNLAERFYLSWCLREAIL
                     KSQGVGIVKLSEVKHLPLEKQIFSAHCPKGKLHFVSELPFYLSYFYQQPQNMLLSEPL
                     LYHWQNGQFQPIKCQSLVYDVN"
     gene            1091..1510
                     /gene="psiE"
                     /locus_tag="PXD81_002387"
     CDS             1091..1510
                     /gene="psiE"
                     /locus_tag="PXD81_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HDL5538366.1"
                     /translation="MPKKSLEEHHSSFGKIASETFQWIMNIALLVTGALLSYSLFAEA
                     YSLYELSFSDSEKFQIVEKIVIFFLYFEFLALIVKYFKSNYHFPLRYFLYIGITAMVR
                     LIIVDHSNALHTLLFALSILVMIIALYIVHTDRLHKS"
     gene            1519..>2119
                     /locus_tag="PXD81_002388"
     CDS             1519..>2119
                     /locus_tag="PXD81_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HDL5538367.1"
                     /translation="MKLTQYQHNDLQILNRETVYKGHFELQKITFRHKLFSGEMSGEV
                     VRELLIKGEAAALIAYDPTLDKVVLIEQVRIGAYDAGQKKSPWLLELVAGMVDKGNEE
                     PAEVAMREAKEEAGLEVTTVQHALSVWDSPGGQLERLHLFLGLIDSTQVKSGEVHGLL
                     EEENEDILVHVVSREQAYQWVEDGTIDNVIAVIGLQWLQL"
BASE COUNT          603 a          429 c          457 g          630 t
ORIGIN      
        1 gcttacaagg cgtagattct gatttcgcca cttttattcg cacggtagtt attattattg
       61 cacttattgc ctttctcact tacagcggaa aatggcagcc gttagatagc ttgagcagca
      121 aaaactggct gtttttaatc ttatccggct tggcaacagg attgtcttgg tcggcgtatt
      181 tcaaagcatt gcaaatgggt aatgcttccc aagtcgctcc aatcgataaa ttcagcttgg
      241 tgttagttac cctttttgca gtaattttct taggcgaacg cccaagcgga caagattggc
      301 ttggcattgg cttagttgct ggtggcgtac tgttacttgc cttcaagcgg tgatttttat
      361 gcaaaatttt gcaaatattg aaattgcgtt tattcatcgg aaagcacctc tgccgagtga
      421 tttctcctac cctgaaatgc ccggcggttt aactgaaaaa caggtgcaaa aatggcaaag
      481 ccgccgagtc gccttttttc tgctgacgga attgtttaaa aaacaccatc tggatttaga
      541 tttattagct gatatgcaac gctccccaag cggtcgcact tttgtcgatt ctgagcaaat
      601 tgactttaac atcagccatt caggggaatg gatagcggtc attttttcgt attcttttgc
      661 aaaattagcg gtcggcattg atattgaaca cccacaaaaa atcagacgtt accacgattt
      721 aatctgccat tacggaaacg ctgaagaaca aaatgtttta ttagccaacc actcccccat
      781 tctaagcaac ctcgccgaac gcttttattt gagctggtgc ttgcgagagg cgatattgaa
      841 atcgcaaggg gtggggattg tgaaactgtc cgaagtaaaa catttaccgt tagaaaaaca
      901 gatcttttcc gcccattgtc cgaaaggcaa gctgcatttt gtgagcgaac tgccgttcta
      961 tttaagctac ttctatcaac aacctcaaaa tatgctatta tctgaacctt tgctatacca
     1021 ttggcaaaac gggcagtttc agcctataaa gtgccaatca cttgtttatg atgttaatta
     1081 aggataacga atgcctaaaa aatctttaga agaacaccat tccagttttg gcaaaattgc
     1141 ttccgaaacc tttcagtgga ttatgaatat tgccttgcta gtaacaggag cattgctttc
     1201 ttactcgctg tttgctgaag cctattcact ctatgaactt tcctttagcg acagtgagaa
     1261 attccaaatt gtagaaaaaa tagtaatttt ctttctctat tttgagtttc ttgcgttgat
     1321 tgtgaaatat ttcaaatcga attatcactt tccgttacgc tatttcctct atatcgggat
     1381 tactgccatg gtgcgtttga ttattgtcga tcattctaat gcgttgcata cactattatt
     1441 cgcactctct attttggtga tgattatagc attatatatt gtacacaccg atcgtttaca
     1501 taaaagctag gaactaatat gaagctaacc caatatcagc acaatgattt acagattcta
     1561 aaccgagaaa ccgtctataa agggcatttt gagctacaaa aaatcacttt ccgccataag
     1621 ctgttttccg gtgaaatgag cggtgaagtg gtgcgtgaac tcttaattaa aggcgaagcg
     1681 gcggctttaa ttgcctatga tccaacctta gataaggtag tattaattga gcaagtgcgg
     1741 attggagctt acgatgcagg acagaaaaaa tccccttggt tgctggaatt ggttgccggt
     1801 atggtagata aaggcaacga agagccggca gaagtggcaa tgcgtgaagc caaagaagaa
     1861 gccggcttgg aagtcacaac agtgcaacac gccttaagcg tatgggacag tcccggtgga
     1921 caactggaac gcttgcacct gtttctcggc ttgattgaca gcacccaagt gaaatccggc
     1981 gaggtacacg gcttactgga agaagaaaat gaagacattt tggttcacgt tgtaagccgt
     2041 gaacaggcat atcaatgggt agaagacggc acaattgaca atgtgattgc ggtaattggg
     2101 ctacaatggt tgcaattaa
//
LOCUS       DANLYI010000260         2098 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.262, whole genome shotgun sequence.
ACCESSION   DANLYI010000260 DANLYI010000000
VERSION     DANLYI010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2098)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2098)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2098
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.262"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            93..788
                     /locus_tag="PXD81_002389"
     CDS             93..788
                     /locus_tag="PXD81_002389"
                     /EC_number="5.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HDL5538368.1"
                     /translation="MARNIIGILGGMGPAATADMFQKFIRLTPADCDQAHIPLLISSI
                     PDIPDRTRYILANGENPAPVMEKYIEGLESAGATCIIIACNTAHYWFEHLKAKSQVEM
                     LSMIDCVVDEVLKCGKWRVGLLATDATLAVDLYKKQLEAQGLTFICPTENGQKAVMDS
                     IYLLKSGELEKATELMLQQRDELIRLGAEVIILGCTEVPIILAQEIEREPMRYVDSTL
                     VLVKKAIDWYQTH"
     gene            804..>2098
                     /locus_tag="PXD81_002390"
     CDS             804..>2098
                     /locus_tag="PXD81_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter"
                     /protein_id="HDL5538369.1"
                     /translation="MIYAEIFVVLAAIYLGLRSGGLGIGLYGGLGLAILTLGFGLPMG
                     SIPVDVMLIIMTVVIGAAVLQAVGGMDLLVRYAEVLMRKNPKYINFIAPMITWLMTIM
                     AGTGFIVFSTLPVIAEVAKESGIRPSRALAGSVVSSQLAIAGSPLSAAMAAMIAVMEN
                     NSVTFFQVIAVCLPVSFIASMVAAFVASKQGCELQNDVVYLERLKAGIVSPSRKLASE
                     STYSKGAKLSIGIFLLATVLVVLLAAVPGLKPVYENGKSMSTRDIIIVMMLASSCLMM
                     LVGKIKPDTIVASPIFRSGMTSIAVIIGIVTLGMTFVDAHLIEIKASIGNILTEYPML
                     LAVVLFFTCALLYSQGSTSALIIPLAVSLGIPNWAILASFIAITGIFILPTYPTSLAA
                     MELDTTGSTRAGKYIIDHPFMLPALVGVAVGLAFGFVWAP"
BASE COUNT          582 a          337 c          469 g          710 t
ORIGIN      
        1 tttgcatact gattcgattt attgcatttc ttttttgcat aacttgtttg tataagtcat
       61 cgcagtaaat taaatcttat aaggagaagt gtatggcaag aaatattatt ggcattttag
      121 gtggaatggg acctgcagca acagcagata tgttccaaaa atttatccgc ttaacaccag
      181 ctgattgtga tcaagctcat attccattat tgatttcctc tattcccgac ataccagata
      241 gaactcgcta tattttagca aatggagaga atccggctcc ggtaatggaa aaatatattg
      301 aagggcttga gagtgcaggg gcaacctgca tcattattgc atgtaatact gcacattatt
      361 ggtttgaaca cttgaaggcc aaaagtcagg tagagatgct aagtatgatt gattgtgtgg
      421 ttgatgaggt tttgaagtgt ggaaaatggc gggttggatt gttggcaact gatgcaactt
      481 tggcggtaga cctatacaaa aaacagcttg aagcacaagg actgactttt atttgcccga
      541 cagaaaatgg tcaaaaggca gtaatggata gtatctattt attaaaatca ggtgagttag
      601 agaaagccac agagctaatg ttacaacaac gtgatgaact cattcgttta ggtgcagaag
      661 taattatttt aggttgtaca gaagtaccta ttattttagc tcaagaaata gagcgtgagc
      721 caatgcgtta tgtagattca acgttggtat tggttaaaaa agcaattgat tggtatcaaa
      781 ctcattaatt taaggagatg gatatgattt atgcagaaat ctttgtggta cttgctgcaa
      841 tctatttagg actgcgttct gggggcttag gaataggact atatggtggt ttagggcttg
      901 cgattctaac tctaggtttt ggtttaccta tgggttctat tccagttgat gtaatgctga
      961 ttattatgac tgttgtaata ggtgctgctg tgttacaagc agtaggaggt atggatttac
     1021 ttgttcgtta tgcggaagta ttgatgcgta aaaatcctaa atatattaac tttattgcac
     1081 cgatgattac ttggttaatg acgattatgg ctggtacagg ttttatcgtc ttttccacct
     1141 tacctgtgat tgcagaagtg gcaaaagaga gtggtattcg cccttcaaga gcattagccg
     1201 gctcggttgt gtcttctcaa ttagctattg caggttctcc attgagtgct gcaatggcgg
     1261 ctatgatagc agtaatggaa aacaacagtg ttacattttt tcaagtaata gcagtatgct
     1321 tacctgtttc ttttattgcc tctatggttg ctgcatttgt tgcttctaag cagggctgtg
     1381 aattgcaaaa tgatgttgtt tatttagaac gtttaaaagc aggaatcgtc agcccatctc
     1441 gtaagttagc gagtgaatct acctattcaa aaggagcaaa actatcgatt ggcattttct
     1501 tactagcaac tgtattggtt gttctacttg cagccgttcc agggttaaag ccagtatatg
     1561 aaaatgggaa gtcaatgagt acccgagata taattattgt gatgatgtta gcctcttcgt
     1621 gcttaatgat gttggtagga aaaattaaac ctgatacgat tgtggctagt ccaattttcc
     1681 gctcaggaat gacttctatt gcggtaatta tcgggattgt tacattaggt atgacttttg
     1741 tcgatgcaca tttaatagaa attaaagcct ctattggaaa tattttaact gaatatccaa
     1801 tgttgttagc tgtagtactc ttttttactt gtgcattact ttattcacaa gggtcaactt
     1861 ctgctcttat tattccatta gcagtaagct tggggatccc taactgggca attttggctt
     1921 ctttcattgc aattacaggc atttttatcc tgccaaccta cccaacttca ttagcagcga
     1981 tggaattaga tactacgggt tcaactcgtg ctggcaaata tattatagat catccgttta
     2041 tgcttccagc tttagttggg gttgctgttg ggcttgcttt tggattcgta tgggctcc
//
LOCUS       DANLYI010000261         2039 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.263, whole genome shotgun sequence.
ACCESSION   DANLYI010000261 DANLYI010000000
VERSION     DANLYI010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2039)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2039)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2039
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.263"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            complement(134..352)
                     /gene="ilvM"
                     /locus_tag="PXD81_002391"
     CDS             complement(134..352)
                     /gene="ilvM"
                     /locus_tag="PXD81_002391"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HDL5538370.1"
                     /translation="MEEYQLTLKANKRPETLERILRVIRHRGFEVKCLNVESTDSAFN
                     LNFTLASKRPLSLLSHQLEKLFDIIEIN"
     gene            complement(362..2014)
                     /locus_tag="PXD81_002392"
     CDS             complement(362..2014)
                     /locus_tag="PXD81_002392"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014992040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HDL5538371.1"
                     /translation="MNGARFITESLKAHGVTTLFGYPGGAIMPTYDAIYDSGLDHLLC
                     RNEQGAAMAAIGYARATGKVGVCIATSGPGATNLITGLGDAALDSIPVVAITGQVASH
                     LIGTDAFQEADVLGLSLACTKHSFIVQNIEELPEILALAFQIAQSGRPGPVLVDIPRD
                     VQLAQTSALPIVYPKEPADAQDPNKLVVAKQLLATAKRPVLYVGGGVSMADGVQAVRN
                     FVKIANIPSVSTLKGLGTIDPTDPLYMGMIGMHGTKAANYAVQECDLLIACGARFDDR
                     VTGKLDTFAPHAKVIHIDIDNAEINKLRQAQVALKGNLIEAVNYLAQPLDIEPWREDV
                     RNLKQQFDFQYQENSGDGNIDAVALLHTLSQRKAKNSIVTTDVGQHQMWSAQHLTYHD
                     PKNYITSAGFGSMGFGLPAAIGAIKARAEDQVILITGDGSIMMNIQELGTLKRADLAV
                     KILLLDNQRLGMVRQWQSLFFHGRHSQTILDDNPDFVLLAKAFDIDGERIEKASEVSD
                     AIDRMFNAKGAYLLHVCIPQEDNVWPLVPPGACNADMLDDEV"
BASE COUNT          587 a          444 c          429 g          579 t
ORIGIN      
        1 gaaaataaag agcaataagt gaaaaaaaag agttgaaatt tgaagaaatg aataaaatca
       61 aaaggatagc gaatgataaa ttttaagcat aaaaaagccg tgtatttttt tacacggcga
      121 atgaaaagtc tgattaattg atttcaatga tatcaaacag tttttctaat tgatggctta
      181 aaagagagag tggacgttta cttgctaatg taaaatttag gttaaaggcg ctgtctgttg
      241 attctacatt taagcatttc acctcaaaac cacggtgacg tataacccgt aaaatacgct
      301 ctaaggtttc agggcgtttg ttagctttaa gcgttaattg atattcttcc atcttattct
      361 cctatacctc atcgtctaac atatcggcat tacacgcccc cggcggtacc agtggccaaa
      421 cgttatcttc ctgtggaata caaacgtgaa gtaagtaagc ccctttggca ttgaacattc
      481 tgtctattgc atctgacact tcgctcgctt tctcaattcg ctcaccgtca atatcaaagg
      541 ctttcgccaa caacacaaaa tcagggttat catctaaaat ggtttggctg tgtcgcccgt
      601 ggaagaacag cgattgccat tggcgaacca tacctaaacg ttggttatcc agtaatagga
      661 ttttgaccgc taaatcagct cttttaagcg tgccgagttc ttgaatattc atcataatcg
      721 agccatcacc ggtaattaaa ataacctgat cttccgctcg agctttgatt gccccaatcg
      781 ccgcaggcaa gccaaaaccc atagagccaa aaccggcaga ggtgatatag tttttcggat
      841 cgtgataagt taaatgttgt gccgaccaca tttgatgctg ccctacatcg gtagtgacga
      901 tgctgttttt ggctttccgc tgtgaaagcg tgtgtaataa cgccacggca tcaatattgc
      961 catcaccact attttcttga tattgaaagt caaattgctg ctttaaatta cgcacatctt
     1021 cacgccatgg ttcaatatct aacggttgag ccaagtaatt cacggcttca atcaaattcc
     1081 cttttaaggc aacttgtgct tgacggagct tgttgatttc cgcattgtcg atatcaatgt
     1141 gaatcacttt agcgtgaggg gcaaaagtgt ctaatttgcc ggtaacacgg tcatcaaagc
     1201 gggcaccaca ggcaatgagt aaatcacact cttgtaccgc gtaatttgct gcttttgtgc
     1261 cgtgcatacc gatcattccc atatagagcg gatcggttgg atcgatagtg cctaaccctt
     1321 ttaaggtaga aacagacgga atatttgcaa tttttacaaa atttctgacc gcttgtacac
     1381 catcagccat actaacgcca ccgccgacat aaagtaccgg gcgtttggcg gtggctaata
     1441 attgtttggc aaccactaat ttgttgggat cttgtgcatc ggcaggttct ttaggataaa
     1501 cgataggcag agccgaagtt tgagctagtt gcacatcacg tggaatatcg accagcaccg
     1561 gaccaggtct gccactttgt gcaatttgga atgcgagggc taaaatttca gggagctcct
     1621 caatattctg aacaataaaa ctgtgtttag tgcaagcgag tgacaaacct agtacatcag
     1681 cttcttggaa ggcatcagtg ccgattaaat ggctggcaac ttgtccggta attgctacca
     1741 caggaataga atcaagtgcg gcatcgccta aaccggtgat taaattagtt gccccagggc
     1801 cggaggtcgc aatacaaacc cctactttac ctgttgcacg ggcataaccg atagctgcca
     1861 ttgcagcacc ttgttcattg cggcaaagta aatgatctaa gcctgaatca taaattgcat
     1921 cataggtagg cataatagcc ccgcccggat agccaaaaag tgtcgttacc ccgtgagctt
     1981 ttaaactctc ggtgataaat cttgctccat tcatggatgt tgtgtcctta atattttta
//
LOCUS       DANLYI010000262         2038 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.264, whole genome shotgun sequence.
ACCESSION   DANLYI010000262 DANLYI010000000
VERSION     DANLYI010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2038)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2038)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2038
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.264"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            complement(46..675)
                     /locus_tag="PXD81_002393"
     CDS             complement(46..675)
                     /locus_tag="PXD81_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005620726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid transporter"
                     /protein_id="HDL5538372.1"
                     /translation="MDIFIQGFIVCFGLIVSIGAQNAFLLKQGILKQHIFWIALLCFL
                     GDVFLMTIGVLGLGTLIAELPVVSFIISLLGAIFLLSYGSRSFISVFKSGEYLVASGE
                     NATSLKKALLITFAITFLNPHVYIDTVVIVGSIGGKLDFNGKIYFLAGALLCSFIWFF
                     GIGYGASLLSPYFAKRRTWQILDAVTGLIMYFIAFSLLIYTYQLALQIF"
     gene            779..1426
                     /gene="udk"
                     /locus_tag="PXD81_002394"
     CDS             779..1426
                     /gene="udk"
                     /locus_tag="PXD81_002394"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HDL5538373.1"
                     /translation="MTDTSQTSSCVVIAIAGASASGKSLIASTIYKELKNELGTDNIG
                     IISEDAYYRDQSHLTMEERIKTNYDHPNSMDHHLLVEHLRQLKQGNAIEIPEYDYTEH
                     NRKTTTKHFEPKKIIILEGILLLTDENIRNEINVSIFVDAPLDICFIRRLQRDMVERG
                     RSMESVISQYRKTVRPMFLQFIEPSKQYADIIVPKGGKNRIAIDILKAQIKQLLK"
     gene            1441..2022
                     /gene="dcd"
                     /locus_tag="PXD81_002395"
     CDS             1441..2022
                     /gene="dcd"
                     /locus_tag="PXD81_002395"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HDL5538374.1"
                     /translation="MRLCDTDIKRYLDEGIIAITPRPSDDKISGATADVRLGNSFRVF
                     REHKTPYIDLSGPREEVAAQLNKVMSDEIIIGDDEAFFLHPGDLALATTLESVSLPAN
                     IVGWLDGRSSLARLGLMVHVTAHRIDPGWEGKIVLEFFNAGKLPLALRPNMAIGALSF
                     EILSGDAAKPYNARKDAKYKNQQSAISSRINQD"
BASE COUNT          709 a          395 c          352 g          582 t
ORIGIN      
        1 tagcaaaaaa gcccgtcatt catcaatgaa aaaatttaaa ctccactaga aaatttgcag
       61 agcaagttga taagtataaa tcagtaaact aaaagcgata aagtacataa ttagcccggt
      121 aacagcatct aaaatttgcc aagttcttcg ctttgcaaaa taaggggaaa gtaagcttgc
      181 tccataacct atgccaaaaa accaaataaa tgagcaaagt aatgcacctg ctaaaaagta
      241 tattttaccg ttaaaatcta atttcccacc gatactgcct acaatcacaa ccgtatcaat
      301 ataaacgtga ggatttaaga atgtaatcgc aaaggtaatc agtaaagcct tttttaagct
      361 cgttgcattc tctccacttg ccacaaggta ttcaccactt ttaaatacgc tgataaatga
      421 acggctaccg tagcttaata gaaaaatggc ccctaaaagc gaaataatga aacttacaac
      481 aggcaattcc gcaattaacg tgcctaagcc tagcacacct atcgtcatta aaaatacatc
      541 acctaaaaaa cagagcaatg caatccaaaa tatgtgttgt tttaaaatcc cttgctttaa
      601 taaaaaggca ttttgtgcgc caatagagac aattaagcca aaacacacta taaatccttg
      661 aatgaaaata tccatttttt ctccaaaatc gcataacaag cggtcaatta tacaaaaaat
      721 tttgcaaaac ttcggtataa tttatcgaat actcttttga ctgaatagga aattttttat
      781 gactgatact agccagactt catcttgcgt tgtcattgcg attgccggcg catctgcctc
      841 aggtaagagc ctgattgcct caaccatcta taaagaactc aaaaatgaat tgggaacaga
      901 taatatcggt attatttccg aagatgctta ttatagagat caatctcatc taacaatgga
      961 agaacggatt aaaaccaatt acgatcaccc taattcaatg gatcatcatt tattggttga
     1021 acatttacgc caattaaaac aaggtaatgc gattgaaatt ccagaatatg actataccga
     1081 acataatcgt aaaacgacca cgaagcactt tgaaccaaag aaaatcatta ttttagaagg
     1141 tattttattg ctgacagatg agaatattcg caatgaaatt aatgtttcta ttttcgtaga
     1201 tgctcctctg gacatctgct ttatccgccg cttacaacgt gatatggtgg aacgtggacg
     1261 ttcgatggaa tcagtgatta gtcaatatcg caaaaccgtt cgcccaatgt tcttacaatt
     1321 tatcgaaccc tctaaacaat atgctgatat tattgtgcca aaaggtggca aaaaccgtat
     1381 tgccattgat attcttaaag cacaaattaa gcaattacta aaataaaata ggagctaaat
     1441 atgcgtttat gtgatacaga tattaaacgt tacttagatg aaggtattat tgccataacg
     1501 cctcgacctt cagacgataa aatttctggc gccactgcag atgtgcgttt aggtaattcg
     1561 tttcgtgtat ttcgtgaaca taaaacgccc tatattgatt taagtggccc tagagaagaa
     1621 gttgccgctc agttaaataa agtgatgagt gatgaaatta tcatcggtga tgatgaagca
     1681 tttttccttc atcccggtga ccttgcactc gcaacaaccc ttgaatcggt ttccttacca
     1741 gccaatatcg ttggatggtt agatggtcgt tcgtcacttg ctagattagg cttaatggta
     1801 cacgttaccg cccacagaat cgatccgggt tgggaaggca aaattgtatt ggaatttttt
     1861 aatgctggaa aattacccct agctttacgc cctaatatgg caattggcgc attaagcttt
     1921 gagattttaa gtggtgatgc tgcaaaaccc tataatgcgc gaaaagatgc aaaatataag
     1981 aaccagcaaa gtgccatttc aagtcggatt aatcaagatt aatatagtac atgccttg
//
LOCUS       DANLYI010000263         2028 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.265, whole genome shotgun sequence.
ACCESSION   DANLYI010000263 DANLYI010000000
VERSION     DANLYI010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2028
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.265"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            109..438
                     /locus_tag="PXD81_002396"
     CDS             109..438
                     /locus_tag="PXD81_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF496 family protein"
                     /protein_id="HDL5538375.1"
                     /translation="MSEVNKSFHDMLEYVHLYRLKNKLHRETADNDRKIRDNQKRVLL
                     LDNLTQYITDEMSIEEVRAIISNMRDDYENRVDDYMIRNAELSKQRREIRQKMASHKQ
                     APTNKKA"
     gene            complement(502..1932)
                     /locus_tag="PXD81_002397"
     CDS             complement(502..1932)
                     /locus_tag="PXD81_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIBC"
                     /protein_id="HDL5538376.1"
                     /translation="MSTLTYLQKIGRALMVPVAALPAAALLMGIGYWIDPDGWGTNSQ
                     LAALLIKSGAAIIDNMSILFAVGVAFGMSKEKDGAAALSGLVGYLVITTLLSPGTVAQ
                     LKGIAVDQVPAAFGKINNQFIGILVGVISAEIYNRFYQVELPKALSFFSGKRLVPIVV
                     SFILLYVWPVIFGALVSFGESIKDMGALGAGIYAFFNRLLIPVGLHHALNSVFWFDVA
                     GINDIPNFLGGAKSLAEGKAVIGVTGMYQAGFFPMMMFGLPAAALAIYHSAKPAKKMQ
                     VASIMFAAALASFFTGITEPLEFAFMFVAPVLYVVHALLTGLSVYIAASMEWIAGFGF
                     SAGLVDLVLSTRNPLAVQWYMLLAQGIVFAVIYYVLFRVVIQAFNLKTPGREEDSEVQ
                     TVNTVSRSEQAAKFVEALGGKENITSIDACITRLRLTLNDTAKADETKLKALGSKGNI
                     KLGENGYQVILGPEAEFVADAMKKLN"
BASE COUNT          686 a          460 c          352 g          530 t
ORIGIN      
        1 agaattaatt taaatcataa aatgtgataa atttttttga cgaattgacg gaaaaagaat
       61 aggatttacg agcttactta attcaacact acatcaaggg agattgatat gtctgaagtg
      121 aataaatcat tccacgatat gctggaatat gtgcatcttt atcgtctaaa aaacaaactg
      181 caccgtgaaa cggcggataa tgatcgcaaa attcgagata atcagaagcg tgtactttta
      241 ttagataacc taacgcaata tattaccgac gaaatgtcta tcgaggaagt tcgagcgatt
      301 atttccaata tgcgtgatga ctatgaaaat cgagtagatg attatatgat ccgtaatgcg
      361 gaactttcca aacaacgccg tgaaatccgc caaaaaatgg cctctcacaa acaagcacca
      421 accaataaaa aagcataaaa agccaagcgg tctgatttgc aaaaaaatat gcaaatcaga
      481 ccgcttgtga ttaagcagag attaattcag ctttttcatt gcatctgcca caaattctgc
      541 ttccggtccc aaaatcactt ggtagccatt ctcgcctaat ttgatattac cttttgaccc
      601 taacgctttc aatttggttt cgtctgcttt cgccgtatca tttaaggtta ggcgtaagcg
      661 ggtaatacaa gcatcaatgc tggtaatatt ctctttacca cctaatgctt caacaaattt
      721 ggcagcttgt tcgctacgag aaacagtatt cactgtttgt acttcgctat cttcctcacg
      781 ccccggcgtt tttaaattaa aggcttggat cactactcgg aacaatacat agtaaattac
      841 tgcgaatacg ataccttggg ccaataacat ataccactgc actgccagcg gatttcgtgt
      901 cgataacact aaatcgacca accctgcact aaaaccgaaa ccggcaatcc attccattga
      961 ggctgcaata taaacagata atccggttaa taatgcgtga accacataaa gcacaggtgc
     1021 aacaaacata aaggcaaatt caagcggttc cgtaatgccg gtgaagaacg aagctaaagc
     1081 agcagcaaac ataatagagg ctacttgcat tttcttagcc ggtttcgcac tgtggtaaat
     1141 agctaatgct gccgcaggta agccaaacat catcatcggg aagaaaccag cttgatacat
     1201 acctgttact cctatcactg ctttgccctc agccaatgat tttgccccac ctaagaagtt
     1261 tggaatatca ttaatgcctg caacatcaaa ccagaaaacc gagtttaatg cgtggtgtaa
     1321 tccaaccgga attaataaac gattaaagaa agcataaata cctgccccta acgcccccat
     1381 atctttgata gattccccaa aagagactaa agcaccaaaa ataaccggcc agacataaag
     1441 caagataaat gacaccacaa tcggcaccaa tcgtttaccg ctaaagaaag acaatgcttt
     1501 cggtaactct acttggtaga aacggttata aatttcggct gaaataaccc ctactaagat
     1561 cccgataaat tggttgttga ttttaccgaa tgctgcaggc acttgatcaa ctgcgatccc
     1621 ttttagctga gcaacggtcc ccggagacaa caaagtggta attactaaat acccgactaa
     1681 accggataat gctgctgccc catctttttc ttttgacata ccaaatgcca cacccacggc
     1741 aaatagaata gacatattat caataattgc cgccccagat ttaatcagca atgccgctaa
     1801 ttggctattt gttccccagc cgtccggatc aatccaatag cctataccca tcaataatgc
     1861 tgctgctggt aatgcagcaa ccggcaccat taatgcacgc ccaatttttt gtaaataggt
     1921 taatgtactc ataaaagcct ccaaaaatac ctcataatga gtaacttaag catagaaaaa
     1981 aaccgattaa agaacaatca aataaatttg gttttgtgat ccatttca
//
LOCUS       DANLYI010000264         2000 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.266, whole genome shotgun sequence.
ACCESSION   DANLYI010000264 DANLYI010000000
VERSION     DANLYI010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2000
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.266"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            complement(3..695)
                     /locus_tag="PXD81_002398"
     CDS             complement(3..695)
                     /locus_tag="PXD81_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDL5538377.1"
                     /translation="MILFAGDPHGSYEHLYPILQQQTEEVALVILGDLQLTTPDELDR
                     LSQYCDIWFIHGNHDSKTVAAFDAIWNSSWKERNIHGKVVDIQGIKIAGIGGVFRGHI
                     WMPPNRPMFFDPIHFCQYSAPEKIWRGGIPLRHRSSIFPSDFEALENEKADILITHEA
                     PKPHPQGFSVINQLARKLGASKIFHGHYHDNFNYGNLNVSRECAIYNIGFRSLADING
                     NYLILGVDDREK"
     gene            complement(713..1987)
                     /gene="nadR"
                     /locus_tag="PXD81_002399"
     CDS             complement(713..1987)
                     /gene="nadR"
                     /locus_tag="PXD81_002399"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HDL5538378.1"
                     /translation="MTDFAYLQQKRKQLKLKVNDICEQAGVTRAYFNQLVSGKIKNPS
                     ANKLKALHSVLDIVEDSNQRSGVIFGKFYPIHTGHINMIYEAFSKVDVLHVIVCTDSE
                     RDLRLFQESKMKRMPTNEDRLRWVQQIFKYQQKQIFIHHLVEDGIPSYPNGWEDWSGR
                     VKALFEEKSINPTIVFSSEIQDKESYEKYLNLEVQLVDPTRESFNISATQIRNNPFQY
                     WRFIPKEVRPFFVKTIAILGGESSGKSVLVSKLANVFNTTSAWEYGREFVFEQLGGNE
                     QAMQYSDYPQMVLGHQRYVDYAMKHAHKVAIIDTDYITTQAFCIQYEGKAHPFLDSMI
                     REYPFDVTILLSNNTKWVADGLRSLGSKKQRQQFQHLLKKLLEKHHIPYIKIESPSYL
                     DRYNQAKEVVEAILNEEPIPLQFKQYNIHEFE"
BASE COUNT          604 a          393 c          365 g          638 t
ORIGIN      
        1 ggctatttct ctcgatcatc aacacctaaa attagataat ttccattaat atccgctagg
       61 ctacgaaagc cgatattata aatagcacat tctctgctga catttagatt gccataatta
      121 aaattatcat ggtagtggcc atggaaaatt ttgctcgccc ctaattttct tgctagctgg
      181 ttgattacag agaagccttg agggtgtggc tttggtgctt cgtgtgtaat taaaatatcg
      241 gctttttcat tttccaatgc ttcaaaatca gaagggaaga tcgaagagcg gtgccttaat
      301 ggaataccac cacgccaaat tttttccgga gcagagtatt ggcaaaaatg gatgggatca
      361 aaaaacattg gacgattggg cggcatccaa atatgacctc ggaagacccc tccaatacct
      421 gcaattttta ttccttgaat gtcaactact ttaccgtgaa tattgcgttc tttccagcta
      481 ctattccaaa tagcatcaaa ggcagcaacg gttttactgt catggttacc atgaataaac
      541 caaatatcac aatactgcga aagccgatct aattcatcag gagtagtcaa ttgaagatcg
      601 cctagaataa ctaaagctac ctcctccgtc tgctgttgta gaatcggata aagatgctcg
      661 tagctaccgt gtggatcgcc tgcaaaaagt atcatgttat ttcctgtggc agttattcaa
      721 attcgtgaat attatattgc ttaaactgta atgggattgg ttcttcattt aaaatagctt
      781 ctaccacctc tttggcttga ttgtagcgat ccaaataact tggagattca attttaatat
      841 aaggaatatg gtgtttttct agtagttttt tcagtaggtg ttgaaactgt tgacgctgtt
      901 ttttcgatcc taaactgcgt aaaccatcgg ctacccattt tgtattgtta gaaagtaaaa
      961 tcgtcacatc aaaaggatat tcgcggatca ttgaatccag aaacgggtga gccttccctt
     1021 catactgaat acagaacgct tgagttgtga tgtaatcggt atcaataata gccactttat
     1081 gagcatgttt catggcataa tcaacgtagc gttgatgtcc aagcaccatt tgcggatagt
     1141 cggaatattg cattgcttgt tcattgccac caagttgctc aaacacaaac tcacgcccat
     1201 attcccaagc agaggtagtg ttaaatacat tcgctaattt gctcaccaac actgatttac
     1261 cactgctttc accacctaaa atcgcaatag ttttaacaaa gaaaggacga acttctttcg
     1321 gaataaagcg ccagtattga aaaggattat tacgaatttg tgttgctgaa atattaaagg
     1381 attcacgagt aggatcgaca agttgaactt ctagattcaa atatttttca taagattctt
     1441 tatcttgaat ttcactacta aaaacaatag taggattaat gcttttttct tcaaatagcg
     1501 ctttaacacg accagaccaa tcttcccagc catttggata gctcggaata ccgtcttcaa
     1561 ctaaatggtg aataaagatc tgtttttgtt ggtatttaaa aatttgctgt acccagcgta
     1621 gccgatcttc atttgtcggc attcgtttca ttttactttc ctggaaaaga cgtaaatccc
     1681 gttcagaatc ggtacaaaca ataacatgta aaacatctac tttactaaat gcttcataga
     1741 tcatattaat atgacctgtg tgaataggat agaatttgcc aaaaataacc ccggaacgct
     1801 ggttgctatc ttctacgata tctaaaacgg aatgcaaggc tttaagctta ttggcgcttg
     1861 gatttttgat ttttccgctt actaactgat taaaataagc tcgggttacc ccagcttgtt
     1921 cacaaatatc attcactttg agtttaagct gtttgcgctt ttgttgaaga taagcaaaat
     1981 ctgtcataat gattccctcg
//
LOCUS       DANLYI010000265         1945 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.267, whole genome shotgun sequence.
ACCESSION   DANLYI010000265 DANLYI010000000
VERSION     DANLYI010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1945
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.267"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            complement(<1..430)
                     /locus_tag="PXD81_002400"
     CDS             complement(<1..430)
                     /locus_tag="PXD81_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HDL5538379.1"
                     /translation="MLEKIEKLIDEINKLHLAFSKDYFETGKIEKVNLKHTFAKVPTP
                     HILAYRLNLHESINDYLYRADLEGISYFYRVKTSESILDKIERFKQRSEGYPVNSILN
                     DIFGARIIVSSEVIQQVMESLDYWQEKYGLKNWYLRDKDEY"
     gene            complement(451..1722)
                     /locus_tag="PXD81_002401"
     CDS             complement(451..1722)
                     /locus_tag="PXD81_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HDL5538380.1"
                     /translation="MSIQQKKRHSRWYERIFFSTAVQSILGVMFSSVLPAIYFWGEKF
                     NSIETRSNTIIATSLAFVMIAISLRVLMKYPGNKSASFVFSTVISWYAVLLIVLTIFR
                     FDYSNTLLGTSFILTILFCFIGYFLSRSWIIPKIALVPFGRAINLNRIQNVEWIQLSE
                     PILGEKRINSIAVDLHYPDLSAEWHKFLAKCTLNGIPVYNVRQLEESLTGRVKIRHMY
                     ENDLGSLLPSPIYSLVKRIIDIALIVATFPITLPIMTITAIAIRLESEGGAMFIQKRV
                     GQGGKEFNIYKFRSMCKDSEKDDAQFASSADMRVTRIGKFIRKTRIDELPQFFNVLKG
                     DMSLIGPRPEQTAFVDKFEEEIPFYNYRHIVKPGISGWAQVVQGYAADVDDTKVKVEH
                     DFYYIKHFSLWLDILIIFKTLKTMLTGFGAR"
BASE COUNT          663 a          379 c          286 g          617 t
ORIGIN      
        1 catattcatc tttatctctc aaataccaat tttttagtcc atatttttct tgccaataat
       61 ctaatgactc cattacttgc tgaatcactt cactagaaac aataatacga gctccaaaaa
      121 tatcgttgag aatgctattt actggataac cttcactacg ctgtttaaaa cgctcgattt
      181 tatctaaaat cgattctgag gtttttactc ggtaaaaata agaaattccc tctaaatctg
      241 cacgatataa ataatcatta atagattcgt gtaaattaag acgataagct aaaatatgtg
      301 gcgtaggcac tttggcaaaa gtatgcttaa gattcacttt ttcaatctta cctgtttcaa
      361 agtagtcttt cgaaaaagct aagtgcaact tgttgatttc atcaatgagt ttttctattt
      421 tctcaagcat gctattcact ctctaattta ttatcttgcc ccaaatccag ttagcattgt
      481 tttcaatgtt ttaaaaataa ttaaaatatc aagccataaa gaaaaatgct taatatagta
      541 gaagtcatgt tctactttta cttttgtatc atcaacatct gccgcataac cttggacgac
      601 ctgagcccaa ccggaaatac ccggtttaac gatatggcga taattataga atggaatttc
      661 ctcttcaaat ttatctacaa aggctgtctg ttctgggcga ggaccaatta agctcatatc
      721 accttttaat acattaaaaa actgaggtag ctcatcaata cgagttttac ggataaattt
      781 cccaatacga gttaccctca tatccgcact cgaagcaaac tgtgcatcat ctttttcgga
      841 gtctttacac atactacgaa atttataaat attaaactcc tttccacctt gcccaacgcg
      901 tttttgaata aacattgctc caccttcact ttctaagcga atagcaatag cggtgattgt
      961 cataataggt aaagtaatgg gaaacgtagc aacaatcaat gcaatatcaa tgatacgctt
     1021 cactaatgag taaattggag aaggaagtaa tgaacctaag tcattttcat acatatggcg
     1081 aattttgaca cgcccggtta aagattcctc aagctggcga acgttatata caggaatacc
     1141 attcaatgta catttcgcta aaaatttatg ccattctgcg gacaaatcag gatagtgtaa
     1201 atctaccgca atagagttaa ttctcttttc ccccaatatt ggttcactta attgaatcca
     1261 ctctacattc tgaattcggt tcaaattgat agctcttccg aatggaacca aagcgatttt
     1321 agggataatc caacttcttg aaagaaaata accaataaaa caaaatagaa ttgtgagtat
     1381 aaagctcgta cctagcaatg tattagaata atcaaatcta aatattgtta acacgattaa
     1441 taaaaccgca taccaagaaa taacggtgga aaaaacaaaa cttgctgact tattacctgg
     1501 atatttcatt aacactctta agctgatagc tatcattaca aaggcaagag aagtcgctat
     1561 aatcgtatta gaacgagttt caatagaatt aaatttctct ccccaaaaat agatagcggg
     1621 taagacactt gagaacataa cgcccaaaat actttgaaca gctgtactaa aaaagatacg
     1681 ttcataccaa cgagagtgtc ttttcttttg ctgaatgctc ataatttatc cttttcttaa
     1741 tttcttataa agtcttccta aaagagatct aggcaataac cgtggtaagg ctctaagtat
     1801 aaaaaacatg cttgatgaga ttattgattg taatcccaac tcttttttca attttgctaa
     1861 ttggtactca ctcttaatgt aattccaccc tttacgccga gcgtacattg cagaacctga
     1921 acgaacattt accagtacat ctggt
//
LOCUS       DANLYI010000266         1943 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.268, whole genome shotgun sequence.
ACCESSION   DANLYI010000266 DANLYI010000000
VERSION     DANLYI010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1943
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.268"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            74..448
                     /locus_tag="PXD81_002402"
     CDS             74..448
                     /locus_tag="PXD81_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance protein NlpE N-terminal
                     domain-containing protein"
                     /protein_id="HDL5538381.1"
                     /translation="MKKIALIGFTTAMLGACSLLPQKSVSGTYAGELPCADCEKIQAQ
                     LTLNADKTYQYDTVYFKDKKEYTYRDKGTYSWEPNKPNVIRLEKASGNLAFQVSEQHV
                     ELCDPNGNTVKNSNNYKLGKVR"
     gene            complement(624..713)
                     /locus_tag="PXD81_002403"
     tRNA            complement(624..713)
                     /locus_tag="PXD81_002403"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(677..679),aa:Ser,seq:gga)
     gene            complement(805..1116)
                     /locus_tag="PXD81_002404"
     CDS             complement(805..1116)
                     /locus_tag="PXD81_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA/IbaG family iron-sulfur metabolism protein"
                     /protein_id="HDL5538382.1"
                     /translation="MSVEQTIIQKIKTEFQPIFFNVENESYMHSSGRGAESHFKLTLV
                     SDKFEGQRTVARHRAVYACLAEELANGVHALALHLYTQGEWEATGGLVPKSLNCLGVG
                     Q"
     gene            1208..1795
                     /locus_tag="PXD81_002405"
     CDS             1208..1795
                     /locus_tag="PXD81_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012262747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5538383.1"
                     /translation="MKRSKKTLVLATTFVAALLAGCQSQSNTLTFSTPTPTAVFNTNN
                     QTAAVNVMTQDLRTSREIASYTKNGEVIRLTAVPEVAAMFQQAVQQDLNAKGFQIVQG
                     AANANVVVNIRKFFATVEQGNLRYKADAQVSVKVAVQGSKGNFSKNFNATRGYEGAFG
                     ASNDEIKKVLDQAYSDVVKAIYNDNEIANAIHQFK"
BASE COUNT          663 a          405 c          351 g          524 t
ORIGIN      
        1 acctgaaata acccaacttg aatttagagt aaatccgagt gttacataat ttaattttga
       61 agaggaaaat agtatgaaaa aaatcgcact cattggtttt accaccgcaa tgctaggggc
      121 ttgctcattg ctaccacaaa aaagcgttag cggtacttat gcaggtgaat taccgtgtgc
      181 tgactgtgag aaaatccaag cccagctaac cttaaatgca gataaaacct accaatatga
      241 tacggtatat tttaaagata aaaaagaata cacttaccga gacaaaggca catacagttg
      301 ggaacccaat aagccaaatg tgattcgtct ggaaaaagca tcaggcaatc ttgccttcca
      361 agtgtctgaa caacacgttg aactttgcga cccaaatggc aacacggtaa aaaacagcaa
      421 taactataaa ctaggtaaag tacgttaaaa gcagataaaa aggattctta ggaatccttt
      481 ttctttgtaa tctatcgccg atcgcataaa aaaaccccca agcaatgttt gctcggggat
      541 aacctaaaaa tgaaaaagta ttatctactt taacttaacc aaggaaatat ctatgaaaac
      601 agtaaggaat ctttcagtat gaatggcggt gagaggggga ttcgaacccc cgatacagtt
      661 tcccatatac acgctttcca ggcgtgctcc ttcaaccact cggacatctc accgaatcaa
      721 caaaaagatg gcgtaaatat agcaatttta cacggctctt tcaagtattt tcttcacaaa
      781 ataaaataaa tcatttttat ccacttattg cccaacacct aagcaattta gcgatttagg
      841 tacaagtccg ccagtcgctt cccattcacc ttgggtatat aggtgcaaag caagtgcgtg
      901 aacgccatta gctaattcct cagctaagca agcatagaca gcacgatgac gtgcaactgt
      961 tctctgccct tcaaatttat ctgacaccaa agttaattta aaatgcgatt ctgctcctct
     1021 acctgagctg tgcatatagc tttcgttttc gacattaaag aaaatcggct gaaattctgt
     1081 cttaattttt tgaataattg tctgttctac cgacattttt tgctctcgtt cattgaaaat
     1141 caaaacgaaa taatactata ctaagcagaa attttctcga aatttattca atatagaagg
     1201 atttactatg aaacgctcta aaaaaacgtt agtgttagcc actacttttg ttgccgcatt
     1261 actagccggc tgccaaagcc aatccaacac attaacattc tctaccccaa cgccaactgc
     1321 tgtatttaac acaaacaatc aaacggctgc ggtgaatgta atgacacaag atttacgcac
     1381 ctcgcgtgaa atcgcaagct acactaaaaa cggagaggtc attcgtttaa ccgcagtgcc
     1441 tgaagttgct gcaatgttcc aacaagccgt ccaacaagac ctgaatgcaa aaggtttcca
     1501 aattgtacaa ggtgcagcga atgccaatgt ggtagtgaat attcgtaaat tctttgccac
     1561 agtagaacaa ggaaacttac gctacaaagc cgatgcacaa gtgagtgtaa aagtggcagt
     1621 gcaaggttct aaaggaaact ttagcaaaaa cttcaacgct acccgtggtt acgaaggtgc
     1681 atttggtgca agcaatgatg aaatcaagaa agtgttagac caagcctatt ctgatgtggt
     1741 gaaagctatt tacaacgata atgaaatcgc caatgcaatt caccagttca aataattaat
     1801 tttttctggc aagcggtcat ctttcttcga ttttttgcaa aaaacgtgga aaaatgaccg
     1861 cttgttgttt tctcgttttg tgatgtggct cgaaaaataa gataaattaa cttgattaaa
     1921 cgatcagtag cccccacaat aga
//
LOCUS       DANLYI010000267         1938 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.269, whole genome shotgun sequence.
ACCESSION   DANLYI010000267 DANLYI010000000
VERSION     DANLYI010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1938)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1938)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1938
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.269"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            complement(50..340)
                     /locus_tag="PXD81_002406"
     CDS             complement(50..340)
                     /locus_tag="PXD81_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5538384.1"
                     /translation="MNNLSEFKLRYINNPYSNKDTFFIFDELSILLKSLTSDELKKIG
                     TAKNLADRQLNKQRKRCKRYVSEAYRLVEDNKEPYSKFFNHDGITAYNIKAF"
     gene            complement(390..1154)
                     /locus_tag="PXD81_002407"
     CDS             complement(390..1154)
                     /locus_tag="PXD81_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005704989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDL5538385.1"
                     /translation="MPNITPKLVINALEPAKEKGIGDTLKRAIRLLNEVMNFAVNADV
                     IEFNKCQNVGSNFSVAKSEHNPTIHPKELPQFLAALRDYNLSFQTKMLIKWQLLTMTR
                     SREASETLWSEIDLEAKTWTISAERMKMKRPHVIPLSRQAVEILERMRKITGNSPFVF
                     QSEMKPRQPMNSQTANRAIGLLGYAGTLTAHGMRSIASTYLNEQLVNYDVVEACLAHV
                     IKDQTRKAYNRSDYLDQRVDVMQQWADYVESCSTGI"
     gene            complement(1123..1602)
                     /locus_tag="PXD81_002408"
     CDS             complement(1123..1602)
                     /locus_tag="PXD81_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HDL5538386.1"
                     /translation="MARVTTELNNTQIKNAKAQTKDYKLMDGKGLFLLVKKNGSKLWR
                     FRYKKPITGKESDLGIGSYPETTLAQARTIREEYRALLAENIDPLEHRQRLEAEALAE
                     IENTFYKVCLKWRDKCESRVKNGELQALTLKKNWRILEKYLLPCRLLYSKHHAKTSY"
BASE COUNT          543 a          374 c          362 g          659 t
ORIGIN      
        1 acacattaaa gaggtttcag gacgatataa aaaaggctct ctaaatagat tagaaagcct
       61 tgatattgta tgcggttatc ccatcgtggt tgaaaaactt agagtaaggt tctttattat
      121 cttcaacaag acgataagcc tctgatacat accttttaca tctttttctt tgcttattaa
      181 gttgtctatc tgccagattt ttagctgttc ctattttctt taattcatcg gaagtcagtg
      241 attttaatag tatgcttaat tcatcaaata taaaaaatgt atctttatta gaataagggt
      301 tattgatata tcgtaattta aactcagaaa ggttattcat atttgtacac ctctaattaa
      361 acaagcggtc aaattccgcc aaaaatttac taaatccccg ttgagcagct ttctacataa
      421 tccgcccatt gctgcattac atctacacgc tgatctaaat aatcagaacg gttataagcc
      481 tttcgggttt ggtctttgat aacgtgagct aaacaagcct ctactacatc ataattgact
      541 aactgctcat tcaaataggt tgaggcaata gaccgcatac cgtgagccgt tagagtgccg
      601 gcatagccta ataagcctat ggctctgttg gcggtttggc tgttcatcgg ttggcgtggt
      661 ttcatttcgc tttgaaaaac aaatgggcta ttccctgtga tttttcgcat tctttctaaa
      721 atttcgaccg cttgccgtga aagagggata acgtgagggc gtttcatctt cattctttcg
      781 gctgaaatgg tccacgtttt cgcctctaaa tcaatttcac tccacaacgt ttcgcttgcc
      841 tctcgtgagc gtgtcattgt taatagttgc catttaatca acatcttagt ttgaaatgat
      901 aggttgtaat cacgcaatgc agctaaaaat tgaggtagct cttttgggtg tatcgttggg
      961 ttatgttcag atttcgctac gctaaaattt gagcctacat tttggcattt gttaaactcg
     1021 ataacgtccg catttaccgc aaaattcatc acttcattaa gtagcctaat tgctcgttta
     1081 agggtgtcgc ctatgccttt ttctttagca ggctctaaag cattaataac tagttttggc
     1141 gtgatgtttg gaatagagta gtcggcaagg caaaagatac ttttcaagga ttcgccaatt
     1201 cttcttcagc gttaaggctt gaagttcgcc attttttaca cggctttcgc atttatcacg
     1261 ccatttcaag cagactttat aaaaggtgtt ttctatttcg gctaacgctt cagcctctaa
     1321 tctttggcgg tgttcgagtg ggtcgatatt ttccgctagt aatgccctat attcttctct
     1381 aatcgttctt gcttgggcta gggtagtttc ggggtaactg cctatgccta aatcgctctc
     1441 tttgccggta atcggctttt tatacctaaa ccgccataac ttcgagccgt ttttcttaac
     1501 cagcaaaaac agccccttgc cgtccattag cttgtagtct ttggtttgag cctttgcgtt
     1561 tttgatttga gtgttattta attctgtggt aactctagcc ataaaaattt acctaaaatc
     1621 gcattgtgct ttatcagaat cataagggat ttaagggtat gggtttcata cccttttacg
     1681 ttataccctc aaatataccc ttaaaataag gggatgtaaa cgcaagaaca cgaaagaata
     1741 cgaatgaaga aataatttaa aattgctcta aagccagtat catcaatggt tttgcgtaag
     1801 aatgcgaatg aagaaataag agtggaaaat ttaagttggt cccagcctcc tctgaactcg
     1861 cttataactt attgatttat aaagagataa aataaactct acaaaaatag ggatcatttt
     1921 agggatcatt tacataaa
//
LOCUS       DANLYI010000268         1901 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.270, whole genome shotgun sequence.
ACCESSION   DANLYI010000268 DANLYI010000000
VERSION     DANLYI010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1901
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.270"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            17..967
                     /gene="coaA"
                     /locus_tag="PXD81_002409"
     CDS             17..967
                     /gene="coaA"
                     /locus_tag="PXD81_002409"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005608808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HDL5538387.1"
                     /translation="MSLEKTSKITPFLTFDRQQWADLRKSVPLKLTEQDLKPLLGFNE
                     DLSLEEVRTIYLPLARLIQYYIEENLKRQTVLHRFLGIEPPKVPYIISIAGSVSVGKS
                     TSARILQALLSHYPKERKVDLITTDGFLYPLATLTEKNLLKKKGFPESYDIHSLIRFV
                     SDIKSGKRNVKAPIYSHLTYDIIPNQFNTVDQPDIVILEGLNVLQSGMNYPSSPHNVF
                     VSDFVDFSIYVDAEESLLKKWYINRFLKFRRSAFADPNSYFHHYASLSEQEAIETAST
                     IWEEINGLNLRQNILPSRERANLILIKGQDHAIETVKLRK"
     gene            1120..1659
                     /locus_tag="PXD81_002410"
     CDS             1120..1659
                     /locus_tag="PXD81_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005598787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbE family thiol:disulfide interchange protein"
                     /protein_id="HDL5538388.1"
                     /translation="MNKKFLLPLILFLALSVAFLVQLARNSQGDDPKKLESALIGKTV
                     PSFNLESVLDEKQTIDQNIVKTGKPRLLNVWATWCPTCYAEHEYLTILAKQGVEIVGI
                     DYKDERPKAIKFLANYGNPYKAIIFDPKGSLGLDLGVYGAPETFIIDGNGVIQYRHAG
                     DVNDQVWNQVLKPIYDGLK"
BASE COUNT          596 a          390 c          329 g          586 t
ORIGIN      
        1 aattgagatt ttggctatga gccttgaaaa aaccagcaaa attactcctt ttctcacttt
       61 tgatcgccaa caatgggctg atttacgcaa atccgttcca ttgaagctga ccgagcaaga
      121 cctaaaaccg cttttaggct ttaatgaaga tctttcatta gaagaagtac gcactattta
      181 tttaccgctt gcccgtttaa ttcaatacta tatagaagaa aacttaaaac gccagacagt
      241 cttacatcgc tttctcggca ttgaaccgcc aaaagtccct tacattatta gtattgccgg
      301 cagcgtatcg gttgggaaaa gtacctctgc ccgtattctg caagctctgc tctcccatta
      361 ccctaaagag cgaaaagtgg atttaattac caccgacggt tttttatatc cacttgcaac
      421 gctaaccgaa aagaacttac tcaagaaaaa aggctttccg gaatcttatg atattcacag
      481 cctgattcgc tttgtatctg atattaaatc cggcaaacgc aatgtgaaag ctccgattta
      541 ttcacatttg acctacgata ttatcccaaa tcaatttaat acggtggatc aacccgatat
      601 tgtgatttta gaagggctaa acgtgttaca aagtggaatg aactacccgt ccagcccaca
      661 taatgtattt gtatcggact ttgtggattt ttctatttat gtggatgccg aagaaagttt
      721 gctgaaaaaa tggtatatca accgtttctt gaaattccgc cgcagtgcct ttgccgaccc
      781 aaactcctat ttccaccatt atgcgagttt gtccgagcaa gaagcgattg aaaccgcctc
      841 aacgatttgg gaagagatca acggcttaaa tttacgccaa aatattctcc caagccgaga
      901 gcgtgccaac ctcattttaa tcaaagggca agaccacgcc attgaaacgg taaaattacg
      961 caaataatat tttgcaaata attgggcttt ttcgaccgct tgtaatacct taaaatccgt
     1021 tgaataccac tcaacggatt ttttatttaa atcaatgctt attcaactat tttttggtaa
     1081 gctaacgact ttgagagtga ttgatattag gcatatacaa tgaacaaaaa atttctactt
     1141 cctttaattc tctttttggc gttatctgtg gcatttttgg ttcaactcgc tcgcaactcg
     1201 caaggcgatg atccgaaaaa attagaatca gccttaattg gcaaaacagt tccgagcttt
     1261 aatttagaat cggtattaga tgaaaaacag acaatcgacc aaaatatcgt caaaaccggc
     1321 aaacctcgcc tgcttaacgt gtgggcaact tggtgtccga cttgttatgc cgaacacgaa
     1381 tacttaacca ttttggcaaa acaaggggtg gaaattgtcg gtattgatta taaagacgaa
     1441 cgcccaaaag cgattaaatt cttagccaat tacggcaacc cttataaagc gattattttt
     1501 gacccgaaag gctcactcgg tttagattta ggtgtttatg gtgcacccga aacctttatt
     1561 attgacggca acggcgtgat tcaataccgc cacgccggcg atgtaaacga ccaagtgtgg
     1621 aaccaagtgc ttaaaccgat ttatgatgga ttaaagtaaa tgtttgtatt tcctgctgac
     1681 attaagaaat ctataaaaag caattatcaa agagaatata actttatttt tataatagtt
     1741 ggtttatcaa ctattatagg aataattcta actattttat cctcattttt attgctaaac
     1801 tatttagtta gcattgatgc aagaaattta ttcattaact cattaaacag taacttctca
     1861 accatattta ttcctatagc attattttta tttttcccta t
//
LOCUS       DANLYI010000269         1897 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.271, whole genome shotgun sequence.
ACCESSION   DANLYI010000269 DANLYI010000000
VERSION     DANLYI010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1897)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1897)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1897
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.271"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            complement(<1..426)
                     /locus_tag="PXD81_002411"
     CDS             complement(<1..426)
                     /locus_tag="PXD81_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HDL5538389.1"
                     /translation="MPNFSFTHLYKKHTKELPTVTLLSALIGLSQVSYANPLPLGNTT
                     GTNNLAVGEGSFALGTGSQAIGKNAIATGGNISPQEFNVELENFRDLVNQIEELRTQI
                     AEQEKQGSVNQQLQDALKSQIAQYDTILERVREKQAQQAI"
     gene            complement(620..1849)
                     /locus_tag="PXD81_002412"
     CDS             complement(620..1849)
                     /locus_tag="PXD81_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HDL5538390.1"
                     /translation="MQFREINPSPRLLMCPGPTDADPRVLRAMSAPILGQFDPEFTSL
                     MNETMEMERRLFQTKNEQTYVVNSTSRGGLETVLTAAICPGDKVLIPAFGRFGYLLNE
                     ILARSGADITIIEREWGTVFEPEEIEAELKKGGYKAVAIIHGETSTSMMQPLEEIGKI
                     CKQYDAMLIVDTVATLGGVDIRVDEWGIDACIGGTQKCISAPSGTALITYNKRIEEII
                     AKRKRVEKGIRAESDVDGTLPPIPSNYLDLAQLQDYWSPRRLNHHTETTSAQYGVHEA
                     LRIILQEGLEARFARHILNDKALCAGLEAMGLKIFGDRKHKAPVVTAFHIPEGMSGPA
                     LRSDILNHFGIELATSFGPLDGKVIRIGNMGFSSQKRNVLLTLGALEAVLLSHKVKVP
                     SGDAVAAALAVYKEADK"
BASE COUNT          537 a          366 c          380 g          614 t
ORIGIN      
        1 tattgcttgt tgtgcttgtt tttcacgtac acgctctaaa atagtgtcat attgagcaat
       61 ctgactcttt aaagcatctt gtaattgttg attaacagag ccttgttttt cttgttcagc
      121 aatttgagta cgtaactctt caatttgatt aactaaatca cggaagtttt ctaactccac
      181 attgaattcc tggggggaga tattaccacc ggttgcaatg gcattcttgc cgattgcttg
      241 agaacctgtg cctaacgcaa aactaccttc acctacagca aggttattgg tgccagtggt
      301 gttgcctaaa ggaagtggat tggcataaga gacttggcta agccctatca gagcgctaag
      361 caaggtaaca gtagggagct cttttgtgtg ttttttatac agatgggtga aactaaagtt
      421 tggcatatca aaatttcctc attaaaactt ttacttttca ttcttaaaaa aatgctccgt
      481 gtcataatat ttaatttgat taaatgaaat aggaaaagat taaatttgtg aaaatatttt
      541 gagtttctgt ggttttactg tggataaata tccacagtat taattattag tgagttgtat
      601 tgtaaattta ggaaggtggt tatttatcag cctctttata gactgccaag gcagctgcaa
      661 ccgcatcgcc tgaaggcact tttactttat ggcttaaaag tacggcttct aacgcaccta
      721 aggttaaaag tacgttacgt ttttggcttg agaaacccat attaccgata cggattactt
      781 ttccatctaa ggggccgaat gaggtggcta attcaatacc gaagtggttt aaaatatcac
      841 tacgcaatgc tgggccactc ataccttctg gaatgtggaa tgccgttaca acgggggctt
      901 tatgtttgcg atcgccaaaa attttcaaac ccattgcttc taaacctgca cataatgctt
      961 tgtcgtttaa aatatggcga gcaaaacgag cttctaagcc ttcttgtaaa ataatgcgta
     1021 acgcttcgtg tacaccatat tgtgcagaag tggtttctgt gtggtgattt aaacggcgtg
     1081 ggctccagta atcttgtagc tgtgccaagt ctaagtagtt acttggaatt ggcggtaatg
     1141 tgccatctac atcagactct gcacgaatgc ctttttccac acgtttacgt tttgcaataa
     1201 tctcttcaat ccgtttgtta taagtgatta aagctgtgcc tgatggggcg gaaatacatt
     1261 tttgtgtacc gccgatacaa gcatcaatgc cccattcatc cacacgaata tctacaccgc
     1321 ctaaggttgc aacggtatct acaattaaca ttgcatcata ttgtttacag attttgccga
     1381 tctcttctaa cggttgcatc atagaggttg aggtttcgcc gtgaataata gcaactgctt
     1441 tatagccgcc ttttttcaac tctgcttcaa tttcttctgg ttcaaatacg gttccccact
     1501 ctctttcgat aatggtaata tctgcaccgc tacgagctaa aatttcattt aataaataac
     1561 caaaacgacc aaatgcagga attaacactt tatcgcctgg gcaaatcgct gcggttagaa
     1621 cagtttctaa gccaccacga gaggtggagt tgactacata agtttgctca tttttggttt
     1681 ggaataaacg gcgttccatt tccattgttt cattcattaa tgaggtaaac tcagggtcaa
     1741 attgccctaa aatgggtgca gacattgcac gcaaaacacg tgggtcagca tctgttgggc
     1801 cagggcacat taataaacga ggggagggat taatttctct gaattgcata attatttcct
     1861 ataattgaat gaattaaaaa aaacttatgg attctat
//
LOCUS       DANLYI010000270         1896 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.272, whole genome shotgun sequence.
ACCESSION   DANLYI010000270 DANLYI010000000
VERSION     DANLYI010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1896
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.272"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            305..1837
                     /locus_tag="PXD81_002413"
     CDS             305..1837
                     /locus_tag="PXD81_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HDL5538391.1"
                     /translation="MNLVDYSTSLWSVLPALIALILAIVTRKVIISLSIGILFGAFML
                     TNSNFLDALTYIKNSVVSLVYKGAEEGLNSNNVNIILFLLLLGVLTSLLSISGSNQAF
                     ANWAQKKVKGKRGAKLMAAGLVFFTFIDDYFHSLAVGAIARPVTDKFRVSRAKLAYIL
                     DSTAAPMCVLMPISSWGAYIITLIAGLLATHSITGYSPLGAFMTMSAMNFYAIFAMIM
                     VFFVAYFSFDIGSMARFERQAAHLAELDDEQIETKGHVRDLILPIIALIVATVSMMMK
                     TGADALAESGASFSILGAFENTTVGISLVVGGVAGLIVATICIITSGNISFKNYVRAY
                     GLGVKMMSGAITILFFAWTINGVVSDMQTGKYLSGLVAESIGAEFLPAILFVLAAAMA
                     FSTGTSWGTFGIMLPIGASMAVHSDPALIIPCLSAVMAGAVCGDHCSPVSDTTILSST
                     GAQCNHMDHVISQLPYALLVATASIIGYLVVGFTQSAVLGFIATAVVMAILILLFKEK
                     KH"
BASE COUNT          509 a          311 c          412 g          664 t
ORIGIN      
        1 taaacactgt cctacaaatt gtagaggtgc gaattcaata agtatttctt ctgagtggaa
       61 aacgatgaag gggaaggaaa ggtgaatttg ccgaaatcaa ttaagcgtca tcttaattgg
      121 ttggggtcgt tgtcgaaaga aacgacactg tcgtagtaaa tgctacggag tgctactgtt
      181 agggtgggtt tcagatattt cacatttgcc ttcctttcat atttattttt ataaatatcc
      241 cagtagatct cttgtttggc tattcttatt tatacaaatt atacacagtc attttagagg
      301 ttttatgaat ctagttgatt attctacgtc actttggtcg gtattgccgg cactgattgc
      361 gctaatattg gcaattgtga cacgcaaggt gattatttca cttagcatcg gtattctctt
      421 tggtgcattt atgcttacta atagcaattt tcttgatgca cttacttata ttaaaaatag
      481 cgttgtttct ttagtttata aaggggctga agaaggatta aattcaaata atgttaacat
      541 tattttattt ttgcttttat taggggtatt aacgtcatta ttaagtattt caggtagtaa
      601 tcaggcgttt gcgaattggg cgcagaaaaa ggtaaaaggc aaacgaggcg caaaattaat
      661 ggcggcaggt ttggttttct ttacctttat tgatgactat ttccacagtt tagcagtagg
      721 tgcgattgcc cgccctgtta cggataaatt ccgtgtttct cgtgctaaat tagcttatat
      781 tttagattct accgccgctc caatgtgcgt gttaatgcca atttcaagct ggggggctta
      841 tattattacc ttaatagctg gcttattggc tacgcattcc atcacaggct attcgccact
      901 aggcgcattt atgacaatga gcgcaatgaa cttttacgcc atttttgcga tgattatggt
      961 gttctttgtg gcatatttct catttgatat tggttcgatg gcgcgttttg aacgtcaagc
     1021 agcgcattta gcagaattag atgatgagca aattgaaacg aaaggacacg tgcgcgattt
     1081 gattttgcca attattgcac ttattgttgc tacagtctca atgatgatga aaacaggtgc
     1141 agatgcactg gccgaatcag gtgcttcatt ttctatacta ggtgcttttg aaaatactac
     1201 ggtaggcatt tcattagttg ttggtggtgt ggcagggctt atagttgcaa ctatttgtat
     1261 cattacaagc ggtaatattt cttttaaaaa ttacgtccgt gcttatggct taggtgtgaa
     1321 gatgatgtct ggtgcaatca ctattctctt ctttgcgtgg acaataaacg gcgtagtaag
     1381 tgatatgcaa acagggaaat atctatcagg attggttgca gaaagcattg gagcagagtt
     1441 tttacctgca attctatttg tgttagccgc agcaatggcg ttttcaacag gaactagctg
     1501 gggaacattt ggtattatgt tgccaattgg agcctcaatg gcagtgcatt cagatccagc
     1561 attaattatt ccttgtttat ctgcagtaat ggctggtgcg gtttgtggtg atcactgttc
     1621 tccagtatct gataccacta ttctttcttc aacaggcgca caatgtaatc atatggatca
     1681 tgtcatttct caattacctt atgctttatt agttgcaaca gcttctatta ttggttactt
     1741 agtggttggc tttacccaat cagcggtatt gggatttatt gctacagctg ttgttatggc
     1801 aatactcatt ttgcttttta aagaaaaaaa gcattaattt ccgaaaatgt gaagggaatc
     1861 acagaatgag atttattgat gagataaaat agcact
//
LOCUS       DANLYI010000271         1883 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.273, whole genome shotgun sequence.
ACCESSION   DANLYI010000271 DANLYI010000000
VERSION     DANLYI010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1883
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.273"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            complement(50..1765)
                     /gene="proS"
                     /locus_tag="PXD81_002414"
     CDS             complement(50..1765)
                     /gene="proS"
                     /locus_tag="PXD81_002414"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HDL5538392.1"
                     /translation="MRTSQYLFSTLKEMPNDAQVVSHQLMLRAGMIRPMASGLYNWLP
                     TGIKVLKKVENIIREEMNKGGAIEVLMPVVQPAELWVESGRWNDYGPELLRFKDRGER
                     DFVLGPTHEEVITDLVRREVSSYKQLPLNLYQIQTKFRDEVRPRFGVMRSREFVMKDA
                     YSFHTTQESLQETYDVMYQVYSNIFTRLGLDFRAVQADTGSIGGSASHEFQVLASSGE
                     DDVVFSTESDFAANIELAEAVAVGERQAPTAEMQLVDTPNAKTINELVENHGLAIEKT
                     VKTLVVKGATEEQPLIALVIRGDHELNEIKAQKHPLVADPLEFADEAEIKAKIGAGVG
                     SLGVVNMPIPVIIDRSVALMSDFGCGANIDGKHYFNVNWERDVAVPEIADLRNVVEGD
                     PSPDGKGTLQIKRGIEVGHIFQLGKKYSEAMKATVQGEDGKPLVMTMGCYGIGVTRVV
                     AAAIEQHYDDRGIIWPCDEMAPFTVAIVPMNMHKSEKVQAFAEELYANLKAQGVEVIF
                     DDRKERPGVMFADMELIGVPHMVVIGEKNLENGEIEYKNRRSGEKQMIAKESLLAFLK
                     ANVKA"
BASE COUNT          493 a          489 c          349 g          552 t
ORIGIN      
        1 gctttatatt aaagggcgta ttttatacgc ccaattttgt tatttataat taagccttta
       61 cattcgcttt taagaacgca agtaagctct ctttggcgat catctgtttt tcaccgctgc
      121 ggcggttttt gtattcgatt tcgccgttct cgaggttttt ctcaccaata accaccatat
      181 gcggcacacc gataagctcc atatccgcga acatcacgcc cgggcgttct ttacgatcat
      241 caaaaatcac ctcaacgcct tgagctttta agttagcgta taactcttca gcgaatgcct
      301 gcactttttc cgatttgtgc atattcatcg gcacaatcgc caccgtaaat ggcgccattt
      361 catcacaagg ccagataata ccacgatcat cataatgttg ttcaatcgca gctgccacca
      421 cacgggtaac gccgataccg taacaaccca ttgtcatcac tagcggttta ccgtcttcac
      481 cttgcacagt ggctttcatt gcttctgagt attttttgcc taattggaaa atatgcccta
      541 cttcaatacc tcgtttgatt tgaagtgtgc ctttaccgtc cgggcttggg tcgccttcca
      601 ccacattgcg taaatctgca atttccggca cagcaacatc acgctcccag ttcacgttaa
      661 agtagtgttt gccatcaata ttcgcaccgc agccaaaatc tgacatcaac gccactgagc
      721 ggtcaataat caccggaatc ggcatattca ccacacctaa cgagccaaca ccggcaccga
      781 ttttggcttt aatttccgct tcatcggcaa actcaagcgg atcagccacc aacgggtgtt
      841 tttgagcttt aatttcgttt aactcgtggt cgccacgaat cactaacgca attaacggtt
      901 gctcttcagt tgcccctttc accactaagg ttttcaccgt tttttcaatg gctaaaccgt
      961 gattttctac taattcattg atggttttcg catttggcgt atccactaat tgcatttctg
     1021 ccgttggtgc ttggcgttca cccaccgcaa ccgcttcggc taattcaata ttggcagcaa
     1081 aatctgattc ggttgaaaat accacatcat cttcaccgct tgaggctaac acttggaact
     1141 cgtgagaggc tgaaccgccg atagagccgg tatctgcctg caccgcacgg aaatctaagc
     1201 ccaaacgggt gaaaatattg ctgtaaacct gatacatcac atcataggtt tcttgcaggc
     1261 tctcttgtgt ggtgtggaaa gaataggcat ctttcatcac aaattcacgc gaacgcatta
     1321 ccccgaaacg agggcggact tcatcacgga atttcgtttg aatttggtat aaattcagcg
     1381 gaagttgttt gtacgaagaa acttcacggc gaaccaagtc ggtaatcact tcttcgtgag
     1441 tcgggccaag cacgaaatca cgctcgccac gatctttaaa acgtaataat tccgggccgt
     1501 aatcattcca acgaccggat tccacccata attccgccgg ctgaaccacc ggcattaata
     1561 cctcgatagc cccgcctttg ttcatctctt cacgaataat attttccact tttttcaaca
     1621 ctttgatccc ggtcggcaac cagttgtata aacctgacgc catcgggcga atcattccgg
     1681 cacgcaacat taattgatgg ctaactactt gtgcatcatt cggcatttct ttaagggttg
     1741 aaaataaata ttgacttgta cgcattgcta ttcctattct ttttaaactt tgaaacctga
     1801 aaaaatctgc ttattttaaa acaaagcggt taaattttca ggaaattttg caaaatccta
     1861 taaaaaaata accgcttgta agc
//
LOCUS       DANLYI010000272         1881 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.274, whole genome shotgun sequence.
ACCESSION   DANLYI010000272 DANLYI010000000
VERSION     DANLYI010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1881
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.274"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            complement(344..1585)
                     /locus_tag="PXD81_002415"
     CDS             complement(344..1585)
                     /locus_tag="PXD81_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA domain-containing protein"
                     /protein_id="HDL5538393.1"
                     /translation="MMNKRIYVFADWETFSQPELVGVLSSDVVRGKENFRFSYDNEWL
                     SSPYVQQIDPNLYLYPGEQYSTDTQNFHIFLDSCPDRWGRLLMKRREAALASQENRKP
                     KLLIESDYLLGVHDMNRMGALRFKTELNGPFLDNNTRLAAPPLSSLRELEFAAIQLES
                     SDNVDDPDYLKWLHMLISPGSSLGGARPKASVIDELNQLWIAKFPSRYDDYDIALWEY
                     LAYRLAIQAKIEMATCRIERFNQEHHTFLTQRFDRHCGKRRHFTSALTQLGYYDGDYE
                     ASYLEIAQFLTESGSNTRIDLAQLWRRIVFNIAISNTDDHLRNHGFIYAQEGWILSPA
                     YDLNPVPLSQGLHLNITDSDNRLDYQLAFDVADFFQLSKQQAVQIYDEVLKEINNWQN
                     VAKEIGIPRQEQEQMKFAFNV"
     gene            complement(1585..1788)
                     /locus_tag="PXD81_002416"
     CDS             complement(1585..1788)
                     /locus_tag="PXD81_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HDL5538394.1"
                     /translation="MFTQTLLAQRTGLSRVTVRKIMNGDPTVAIGYYLMVLGVLGLEQ
                     DFTDVGKDDELGRKLQDIELLRK"
BASE COUNT          547 a          378 c          331 g          625 t
ORIGIN      
        1 aaatgttagt ttttctgatg tctgccatct gctaggcatt atatatgcct agattagttt
       61 cctacatttg cgtttttaag gctacgcttc cttgcggata tggttgagaa aaaagtcaag
      121 tgttactaat ggttttataa gatgttggtt tgttctggtg tctgccaccg ctaggtcttt
      181 ataccctaga ttagtatcag gtctttctgg tgcctgccac cgctaggcct ttacatccta
      241 gattaatatc agattgacaa tgagccaaca actcattgcg ttattaaggt aacgctactt
      301 ttttagaaca tagactacga tgattggttc cacaagtcaa ttactataca ttaaatgcaa
      361 atttcatctg ctcttgctct tggcgtggaa taccaatttc ttttgcgaca ttttgccaat
      421 tattaatttc tttcagtact tcatcataaa tctgtacggc ttgttgctta gataattgaa
      481 aaaagtcagc aacatcaaag gcgagctgat aatccaatcg gttatcgctg tctgttatgt
      541 ttaaatgtaa tccttgagat agtggtactg ggtttaaatc gtatgcggga gataatatcc
      601 acccctcttg agcgtagata aatccgtggt tacgcaaatg atcatcagta ttcgaaatag
      661 caatattgaa tacaattctt ctccatagtt gagccaagtc aattcgagta tttgaaccac
      721 tttctgttaa aaattgagca atctcaaggt agcttgcctc atagtctccg tcataataac
      781 ccagctgggt taatgctgac gtaaaatggc gacgttttcc acaatgtcga tcaaatcttt
      841 gtgtcaaaaa agtatggtgt tcttggttaa agcgttcaat tctacaagta gccatttcaa
      901 ttttggcttg aatagccagt cgataagcta aatattccca aagagcaata tcataatcat
      961 cgtaccgact tggaaatttg gcaatccata gttgatttag ttcatctatt accgaagctt
     1021 ttggtctagc tccgcctagt gacgaccctg gtgatatcaa catatgaagc cattttaaat
     1081 aatccggatc gtccacatta tcactgcttt caagttgtat tgctgcaaat tctaattctc
     1141 ttaaggaaga cagtggtggt gctgcaagac gagtattatt gtccaaaaat ggaccattca
     1201 attcagtttt aaaacgtaaa gcccccattc tattcatatc gtgtacgcct aaaaggtaat
     1261 cactttctat tagtaactta ggtttacgat tctcttgact agctagagct gcctcccgac
     1321 gtttcattaa caatctaccc catctatcag ggcaagaatc taaaaagata tgaaaatttt
     1381 gtgtatctgt actgtattgc tctccagggt aaaggtataa gttaggatca atctgctgta
     1441 catagggtga acttaaccac tcgttatcgt agctaaaacg gaaattttct ttgcctctca
     1501 ctacatcaga agaaagcacc ccaactaatt caggctgtga aaaagtttcc caatctgcaa
     1561 acacataaat tcgtttattc atcattattt ccttaataat tcaatatctt gcaatttacg
     1621 tcctaactca tcatcctttc caacatcagt gaaatcttgt tctagaccga gaacacctaa
     1681 aaccattagg tagtatccaa tcgcaacagt tggatcacca ttcataattt ttctcactgt
     1741 tacacggcta agcccagttc tttgagccaa taatgtttga gtaaacattc tcctttttga
     1801 ggctagccta atattttctc caagttgaag caaggcattt tggtacttag gaaacagagt
     1861 tggtgatttt gttttactga g
//
LOCUS       DANLYI010000273         1878 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.275, whole genome shotgun sequence.
ACCESSION   DANLYI010000273 DANLYI010000000
VERSION     DANLYI010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1878
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.275"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            2..493
                     /locus_tag="PXD81_002417"
     CDS             2..493
                     /locus_tag="PXD81_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDL5538395.1"
                     /translation="MQTKPILDACCGSRMFHFDKQNPHVLFADNRQIETTMKDSGKVR
                     HLEIKPDVLHDFTNMPYENESFWHVVFDPPHLVQGGENSWLVKKYGKLPCNWREVLKK
                     GFDECMRVLKPNGTLIFKWNETQIPVSEILKVIDCQPIYGHKSGRLNQTHWLAFVKIE
                     QSL"
     gene            519..794
                     /locus_tag="PXD81_002418"
     CDS             519..794
                     /locus_tag="PXD81_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4224 domain-containing protein"
                     /protein_id="HDL5538396.1"
                     /translation="MEQPTEFLTKDELTDLTGYRQKKRQIEWLRERHYIIQDENKYKP
                     LVLYKDVYGHPRQQYPEAQSTGKWKSPAYLMGVKNGKTTQTTESLLT"
     gene            754..1809
                     /locus_tag="PXD81_002419"
     CDS             754..1809
                     /locus_tag="PXD81_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDL5538397.1"
                     /translation="MGRPRKQQNRYLPENLRPKTVTRKSGKTVIYWLYRTHGKTEISL
                     GTNRNEAFIKAAQLNIERESQTARITFGLVAKRYRDEVIPQKKASTAQTDLCKIKNLL
                     LFFNDAPLEEITPQHIREYMIWRKDTPGSANNEFSMFSHIWQYAREWGYTNLPSPNIG
                     MKRYPIRKREIYVENNIFQMVYEVADQDMRDLMDLAYITGQRPVDLVNIRKEHIFDGY
                     LHIVQQKTQAKLRIEIVGKLADIIQDRLEKATGTHLFSTKKGEKLTARKLGKRFTYIR
                     EQAVKNNPEYANEIMAFQFRDLRAKSGTDKALSLGEEAARQQLGHTSVQMTKTYIRKA
                     PIVTPIKESVPSSKHQS"
BASE COUNT          673 a          371 c          373 g          461 t
ORIGIN      
        1 aatgcaaaca aaaccgatat tagatgcctg ttgtggttcc cgtatgtttc attttgacaa
       61 gcaaaatcca cacgttttat ttgctgataa tcgacaaata gaaaccacaa tgaaagacag
      121 tggcaaagtc cgccatttag aaatcaaacc tgatgtttta cacgacttca cgaatatgcc
      181 atacgaaaac gagagttttt ggcacgttgt ttttgatccg ccacatttag ttcaaggtgg
      241 agaaaatagc tggcttgtga agaagtacgg caaattacct tgcaactggc gagaagtctt
      301 gaagaaaggc tttgacgaat gtatgcgagt gctaaaacca aacggcacgc ttattttcaa
      361 gtggaatgaa acacaaatcc ccgtttcaga aattttaaaa gttattgatt gtcagccgat
      421 ttatggacac aaaagcggac gcttgaacca aacacattgg ctggcgtttg tgaaaattga
      481 gcaatccctc taaattgagg gggttatttg gagagaatat ggaacaaccg actgaatttt
      541 taacgaaaga cgaacttact gatttaacag gctatcgtca aaagaaaagg caaatcgaat
      601 ggttgaggga gcgtcactat ataattcaag atgaaaacaa gtataagcca ttagtgctat
      661 acaaagatgt ctatgggcac ccccgccaac aatatccaga agctcaatcc actgggaaat
      721 ggaaatcacc tgcttattta atgggagtga aaaatgggaa gaccacgcaa acaacagaat
      781 cgctacttac ctgagaattt acgtccaaaa acagttacac gaaaatcagg caaaactgtc
      841 atttattggc tatatcgcac gcacggaaaa acggaaatct ctctaggcac aaatagaaat
      901 gaggctttta ttaaggctgc tcaactcaat atagaaagag aaagccaaac tgccagaatt
      961 accttcgggc ttgtagccaa acgttatcga gatgaagtta tacctcagaa aaaagcgagc
     1021 acagcacaaa cagatttgtg caagataaaa aatctactgc tattctttaa tgacgctcct
     1081 ctcgaagaaa tcacccctca acatatccga gagtatatga tttggcgaaa agacacacct
     1141 ggatctgcta ataacgaatt ttcaatgttc agtcatattt ggcaatacgc tcgagagtgg
     1201 ggttatacaa acttaccaag ccctaatatc ggaatgaaac gctaccctat cagaaagcgt
     1261 gaaatttacg ttgagaacaa tatatttcag atggtatatg aagtggcaga tcaggatatg
     1321 agagacttaa tggatctcgc ctatatcacc ggacaacgtc ctgtcgattt ggtcaatatc
     1381 cggaaagaac acatttttga tggttatctc catattgttc agcaaaaaac acaagccaaa
     1441 ctacgtattg agatagtggg aaaactggct gatattatcc aagataggct ggagaaagct
     1501 actggaacac acttattcag cactaagaaa ggggaaaagc taaccgctag aaaactaggt
     1561 aaaaggttca cttatattcg ggaacaagcg gtcaagaata acccggaata cgcaaatgaa
     1621 ataatggcat tccaatttcg ggatctaaga gctaaatcag gaacagataa agccctttct
     1681 ctaggtgaag aagccgctcg ccaacaacta ggtcatacta gcgttcaaat gaccaaaaca
     1741 tacattcgaa aagccccaat agtaacccct atcaaagaaa gtgttccctc ctcaaaacac
     1801 cagtcttaaa agacagtttc ggaacatttt gcattttttc ggaacgttta cataaaaatc
     1861 acttataata gattgaaa
//
LOCUS       DANLYI010000274         1806 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.276, whole genome shotgun sequence.
ACCESSION   DANLYI010000274 DANLYI010000000
VERSION     DANLYI010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1806
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.276"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            322..576
                     /locus_tag="PXD81_002420"
     CDS             322..576
                     /locus_tag="PXD81_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5538398.1"
                     /translation="MICHADPEYVKYNYAGLWEKGSDGTFFCNYQQFMPEVEAKESLR
                     KQEPELDDRYNEEEYSEEEYPDENDASDENDNYDETYNVF"
     gene            776..952
                     /locus_tag="PXD81_002421"
     CDS             776..952
                     /locus_tag="PXD81_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5538399.1"
                     /translation="MATSSFDKRFVVKDPKAVKVLIEALQSPIKVVLPKKDLEKEEKE
                     ALCAIKKRLASMGL"
     gene            913..1464
                     /locus_tag="PXD81_002422"
     CDS             913..1464
                     /locus_tag="PXD81_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5538400.1"
                     /translation="MCNQKTLSLYGLVKELGIEKTQLFLEQAFSCSRNKDVESFLLNK
                     AIRFETALAAATHLIINEQGDILAYFTLSFKEIEVEVSKSQLKKLTAGLSNDSHLRVL
                     LIGQIGKNEKVIDNPAKLPDILNHIYAQLYKAQRVLGGRVVILECENTPKLLELYKQN
                     KFQILNTIGSENGLKTLFFIPDF"
     gene            1534..1764
                     /locus_tag="PXD81_002423"
     CDS             1534..1764
                     /locus_tag="PXD81_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5538401.1"
                     /translation="MSLLKKAQTFISIFNDVRTKNEAQRQSSNNLNVALKNAAEMRAE
                     MNGFSLKPQKRSTYFADHYGCGIHAVETYDNV"
BASE COUNT          659 a          312 c          310 g          525 t
ORIGIN      
        1 ccgctactca acatacaggg atggttatct tgataacgct cacgatactc ttgagcaata
       61 gctagtaatt gctgattagt catataaagt ctccaataac ataataaacc tgaaaacgtt
      121 cttagtattc tggcaacatt tttataaata gaaaccaaaa actatattca aatcattttt
      181 agtttctgct caacattcct atgtttttcc atacactgaa gtaaatttac ctaaccctat
      241 tttgctgtga aaccaaaact cctcacgctt atccttagta gcatttcgat gtcgctcact
      301 tatgccaccc ctcaattagg gatgatatgc cacgctgatc ctgaatatgt aaaatacaac
      361 tacgccggac tttgggaaaa aggcagcgat ggcacttttt tctgcaatta ccagcaattt
      421 atgcctgaag ttgaagctaa agaatcacta aggaaacaag aaccagaact tgatgataga
      481 tataacgaag aggaatatag cgaagaagaa tatcctgatg aaaatgatgc cagcgatgaa
      541 aacgataact acgatgaaac ttataatgtt ttctagtatg tatatggaaa ccggaggaat
      601 ccgtgattga aaaggcttgc atttccaaat atagtctata tattgtggta attgaaagta
      661 tttctatgct aacaagcata aggctatgta atcgaaaacc acccacaagg cctaaataca
      721 gtattattac agccatgagt aaccatggta atttaaatat ttaaggagtt ctataatggc
      781 aacatcatca tttgataaac gctttgtagt caaagatcca aaagctgtca aggtattaat
      841 tgaggcatta caaagcccaa tcaaagtagt tttacctaaa aaagacttag aaaaagaaga
      901 gaaagaagca ctatgtgcaa tcaaaaaacg cttagcctct atgggcttgt aaaagaacta
      961 ggtattgaaa aaacacaact ctttttggag caagcatttt cttgcagtag aaataaagat
     1021 gttgaaagtt ttctacttaa taaagcaatc agatttgaaa ccgcattagc agcagcaaca
     1081 catttaatca ttaatgaaca gggcgatatt ttggcttact ttaccctctc ttttaaagaa
     1141 attgaggtgg aagtaagcaa aagccaatta aaaaagctaa ctgctggttt aagtaatgat
     1201 tcacatttaa gagtattgct tatcggtcaa atcggcaaaa atgaaaaagt aattgacaat
     1261 ccagccaaat tgccagatat tctaaatcat atctatgcac aactttataa agcacaacga
     1321 gttttaggtg ggcgagtcgt tatattagaa tgtgaaaata cgcctaaatt attagaacta
     1381 tataaacaaa ataaatttca aattctcaat accattggaa gtgagaatgg tctaaaaaca
     1441 cttttcttta ttcctgattt ttaactacag ctaatatttc attagaagaa agtcgccagc
     1501 tttgtcagga agtcatatat gggagaaaga acaatgtctc tacttaaaaa agcacaaacg
     1561 tttatttcta tttttaatga cgtgcgaact aaaaatgaag ctcaacgcca gtcttcaaac
     1621 aatttaaatg ttgccttgaa aaatgctgct gaaatgagag ctgagatgaa tgggttctcc
     1681 cttaagcctc aaaagcgttc tacttacttt gctgaccatt atggttgtgg tattcatgca
     1741 gtagaaacct atgataatgt gtagaagatc agacttgatc tgacaattca ctctaaaaag
     1801 tgagag
//
LOCUS       DANLYI010000275         1782 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.277, whole genome shotgun sequence.
ACCESSION   DANLYI010000275 DANLYI010000000
VERSION     DANLYI010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1782
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.277"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            163..1485
                     /gene="pepP"
                     /locus_tag="PXD81_002424"
     CDS             163..1485
                     /gene="pepP"
                     /locus_tag="PXD81_002424"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HDL5538402.1"
                     /translation="MDLAYLAKMPQEEFTARRERVLEQMQDNSALLVFTESEKRRNSD
                     SEYLFRPDSYFWYLTGLAEPKSAVLLVKSEGKTESIIFVRKKDPLMETWNGRRLGIEE
                     APKTLQLNAAFDIDDIETVLAKKLENLTACYCALGIQEWGDNILFATFEKMKVNRQKV
                     PTTLIDWQPMLSEMRLIKSEFEIALIQQACHISSMAHIRAMKQARPNRYEWEIEGEIQ
                     HEFSRFGARFPAYNSIVAGGENACILHYNENDQVLKGGDLLLIDAGAEFAYYAGDITR
                     TFPINGKFSEPQKELYELTLTMQKEAINLLVPNSSIKAANDKAVQILTQGLVRLGILN
                     GDVESLIEQKAYRQFYMHGLGHWLGLDVHDVGDYGTERDRPLQIGMVLTVEPGIYIPK
                     DADVPEQYKGIGIRIEDNLLITEYGNKNLSSGCPKEIADVEQLMQSLN"
     gene            1601..>1782
                     /locus_tag="PXD81_002425"
     CDS             1601..>1782
                     /locus_tag="PXD81_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1508 domain-containing protein"
                     /protein_id="HDL5538403.1"
                     /translation="MYFEIYKDAKEEFRWRLKAGNHEVIATNQAYTTKQGCQKGIEAV
                     KKVTPETEVKDLTKAEA"
BASE COUNT          567 a          294 c          412 g          509 t
ORIGIN      
        1 ctgtttagat atgtaaaggt tatatttaat cttaatctgt tttgtttttg caatcaagat
       61 ctcaaatata atctttcatc tgttttggtt aggctgaatt tatgctgcgt ttgtgctaaa
      121 ataaggcagg tttcgatata gatataataa aaaggagaaa taatggatct cgcttatttg
      181 gctaaaatgc cacaagaaga atttaccgca cgccgtgagc gtgtgcttga gcaaatgcag
      241 gataattctg cgttattggt ttttaccgaa tcggaaaaac gccgtaatag tgactctgaa
      301 tacttgttta gacctgatag ttacttttgg tatttaaccg gtttggcaga acctaaatca
      361 gccgtattgc tggtaaaaag cgaaggtaaa acggaaagta ttatttttgt gcgtaaaaaa
      421 gaccctttaa tggaaacctg gaatggcaga cgtttaggga ttgaggaagc tccaaaaacg
      481 ttacagctta acgcagcttt tgatattgat gacattgaaa ccgttcttgc aaaaaaatta
      541 gaaaatctga ccgcttgtta ttgtgcgtta gggatacaag agtggggcga taatattttg
      601 tttgcaacat ttgaaaaaat gaaagtcaat cggcaaaaag tgccgacaac cttgattgat
      661 tggcagccaa tgttatcgga aatgcggttg attaaatcag aatttgaaat tgccttaatt
      721 cagcaggcgt gtcatatttc ttcaatggca catattcgag cgatgaaaca agctcgcccg
      781 aatcgttatg agtgggaaat tgagggggaa attcaacacg aatttagccg ttttggagca
      841 aggtttccgg catataattc aatcgttgcc ggcggtgaga atgcctgtat tcttcactac
      901 aatgaaaatg atcaggtgct taaaggcggc gatttattac tgattgatgc aggggcggaa
      961 tttgcgtatt acgccggcga tattacccga accttcccta ttaatgggaa attctccgaa
     1021 ccgcaaaaag agctgtacga attaacctta actatgcaaa aagaggcgat taatcttctt
     1081 gtgccaaaca gctcgattaa agcggcaaac gataaggctg tacagattct cacgcagggc
     1141 ttggttcgct taggaatttt aaacggtgat gtggaaagcc tgattgagca aaaagcctat
     1201 cgccaatttt atatgcacgg attagggcat tggctagggc tagatgttca cgatgtgggg
     1261 gattacggca cggagcggga tcgtcctttg cagattggta tggtgttaac tgttgaaccc
     1321 ggtatctata ttcctaaaga tgcggatgta ccggagcaat ataaaggtat tgggattcgg
     1381 attgaagata atttgctgat taccgaatac ggcaacaaaa acttaagcag cggttgccct
     1441 aaagaaattg cggatgttga gcagttgatg caatcgttaa attaattcgc aaatttttgt
     1501 aaaaaaatga ccgcttgctt tgatattttg gggaatttag cttaagctaa cccgacacaa
     1561 acacagtgtt tgggcaatca caacacgaag gagtgtttat atgtattttg aaatttacaa
     1621 agatgcaaaa gaagaatttc gttggcgttt aaaagcgggt aaccacgaag ttattgcaac
     1681 caaccaagct tatacgacta agcaaggttg tcaaaaaggc attgaagcag ttaaaaaagt
     1741 tacacctgaa acagaagtaa aagatttaac caaagctgaa gc
//
LOCUS       DANLYI010000276         1754 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.278, whole genome shotgun sequence.
ACCESSION   DANLYI010000276 DANLYI010000000
VERSION     DANLYI010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1754
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.278"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            19..465
                     /gene="matP"
                     /locus_tag="PXD81_002426"
     CDS             19..465
                     /gene="matP"
                     /locus_tag="PXD81_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010944225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HDL5538404.1"
                     /translation="MRYQKLITQETTWKWQYLLKKHREGENITKYEEQSLIDLKVQFL
                     STLQNSPDEVEKWIKSEMTPEQRKKMRQSVRAKRKRFFNAEKQTTKKKSIDLEYSSWL
                     RLSKYAKSQKLTLSEAIVKLTDELENKQLYLEQVAKMKSSLKDLLK"
     gene            complement(531..1667)
                     /gene="ompA"
                     /locus_tag="PXD81_002427"
     CDS             complement(531..1667)
                     /gene="ompA"
                     /locus_tag="PXD81_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_119863613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HDL5538405.1"
                     /translation="MKKTLVALAVLSAAAVAQAAPQANTFYAGAKAGWASFHDGLTQF
                     DNTYDYGSGINLGINLGINRNSVTYGVFGGYQILNQNNFGLATELGYDYFGRVRGNTT
                     FSGDDKEKTAAKHSAHGAHLSLKPSYEVVPNLDVYGKVGVALVRNDYYVQQHVDKDSR
                     IKVHNLKPSLLLGAGLEYAITPELAARVEYQYLNRVGNLDKAVRTTVGYLIPRGTNFQ
                     YSPDIHSVSAGLSYRFGQGAAPVEAPEVVTKNFAFSSDVLFDFGKSSLKPAAATSLDA
                     AHAEISNLGLANLAIQVNGYTDRIGKEASNLKLSQRRAETVANYIVSKGTNPANVTAV
                     GYGEANPVTGHTCDAVKGRKALIACLAPDRRVEIQVQGSKEVTM"
BASE COUNT          570 a          331 c          324 g          529 t
ORIGIN      
        1 tcctatcata ttcagattat gcgatatcag aaactcatca cccaagaaac aacttggaaa
       61 tggcaatatc tcctaaaaaa acatcgtgaa ggagaaaata ttactaagta cgaagaacaa
      121 agcttgatcg atttaaaagt tcagttcctc tctactttgc aaaactctcc ggacgaagtg
      181 gaaaaatgga ttaagtcgga aatgacgcct gagcaacgta agaaaatgcg tcaatcagta
      241 cgcgccaaac gcaaacgctt ctttaatgct gaaaagcaaa caaccaagaa aaaatccatt
      301 gatttagagt attcgagctg gttaaggctc tctaaatatg ccaaaagcca aaaactaacg
      361 ctttctgaag ctattgtcaa attaaccgat gaactcgaaa ataaacaact ttacttagag
      421 caagttgcta aaatgaaatc aagtttaaaa gatttactga aataaaaaaa gaaaaaagcc
      481 cactaacaag cgggcttttt ttgattcaat ttttcaactg aataactata ttacatagtt
      541 acttcttttg aaccttgaac ttggatttca acacgacgat ctggtgctaa gcaagcgatt
      601 aatgctttac gacctttaac tgcatcacat gtgtgaccag ttactgggtt tgcttcaccg
      661 taacctacag ctgttacatt agctgggtta gtacctttag aaacgatgta attagctact
      721 gtttcagcac gacgttgtga aagttttaag ttagatgctt ctttaccgat gcggtctgtg
      781 taaccattaa cttggatagc taggtttgct aagcctaagt tgctgatttc agcgtgtgct
      841 gcatctaaag aagttgctgc tgctggtttt aagcttgatt taccgaaatc aaataaaaca
      901 tcagagctga atgcgaagtt tttagttaca acttctggtg cttcaactgg tgctgcacct
      961 tgaccgaaac ggtatgataa acctgcagat actgagtgga tatctgggct gtattggaaa
     1021 tttgtacctc ttggtattag ataaccaact gttgtacgaa ctgccttatc taagttacca
     1081 acacgattta agtattgata ttcaacacga gcagctaatt caggagtaat tgcatactca
     1141 agaccagcac ctaataatag agatggtttt aagttgtgaa ctttaatgcg tgaatcttta
     1201 tctacatgtt gttgtacgta gtaatcgttg cgaactaatg caacacctac tttaccgtaa
     1261 acatctaagt taggaacaac ttcgtagctt ggttttaagc ttaagtgcgc accgtgagct
     1321 gaatgtttag ccgcagtttt ttctttatca tcaccagaaa atgtagtgtt accacgaaca
     1381 cgaccaaagt agtcataacc taattctgtc gctaaaccaa agttgttttg gtttaagatt
     1441 tggtaaccac cgaatacacc gtaagttaca gagttacggt taataccgag gttaataccg
     1501 aggttaatac cggatccata atcatatgtg ttgtcaaatt gagttaaacc gtcgtggaat
     1561 gatgcccaac cagctttagc acctgcgtag aaagtgttag cttgtggagc tgcttgagct
     1621 actgcagctg ctgataatac tgctaatgca actaatgttt ttttcatttt gatgatcctc
     1681 ttaaatattg tcatcgatta aaataaattc ttaataccca caaaaagtat taaaacttta
     1741 actttatgct tcaa
//
LOCUS       DANLYI010000277         1721 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.279, whole genome shotgun sequence.
ACCESSION   DANLYI010000277 DANLYI010000000
VERSION     DANLYI010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1721
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.279"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            369..746
                     /locus_tag="PXD81_002428"
     CDS             369..746
                     /locus_tag="PXD81_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HDL5538406.1"
                     /translation="MDKSQGVVSVNIRGGSGFGRANNAVGPNYMGMLAGKYQFNDSTW
                     IGALFGHSWRKISQNYKIGGGRKVTDSSIDLSKIDDLDKESSTTSPFNAAHLKQKPMS
                     RMAKLEYGDKYQTITLLPKSGSG"
     gene            1413..>1721
                     /locus_tag="PXD81_002429"
     CDS             1413..>1721
                     /locus_tag="PXD81_002429"
                     /inference="COORDINATES: protein motif:HMM:NF012801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HDL5538407.1"
                     /translation="MGEFNCPDVNFPHTATGKHKRINYSATLSAYIHDLFTPFISYSQ
                     THRAPNIKEIFFSNLGDYGVNTALQPEQAKTTQFGINGFKERVFSEEDKLGFKVLFYN
                     T"
BASE COUNT          606 a          295 c          318 g          502 t
ORIGIN      
        1 aatattgaac atgattaata ttttcgtctt gtgctatact agactcgtgc aataaaggca
       61 cacttctttc ttcagtgtct ttagcgttgc gatttaaatt taattacatt ttttatagga
      121 aagacaatag aaatgaagat caaaaaatat gtatttcacc tgacttatac aacacttatc
      181 tgccccttac ctagggtatt cgcagaggaa ggttatatgt tagatacggt tgaggttaaa
      241 gcccaagcaa taaaaaaaca gatgaagttt tcattaaaac aggggcaata agcacccgag
      301 ataaaatctt ggaatcaaac caaagcttag agactattgt tcgttctatg cctggcgcat
      361 ttacacaaat ggataaatct caaggtgtgg tttctgttaa tattcgagga ggttctggat
      421 ttgggcgagc taataatgca gtaggcccaa attatatggg aatgctcgct ggcaaatacc
      481 aattcaatga ctctacttgg ataggagcat tatttgggca tagttggcgc aaaatctctc
      541 aaaattataa aataggaggc ggacgaaaag ttacggatag ctcgattgac cttagtaaaa
      601 ttgatgatct agataaagaa agttctacca cctcaccgtt caatgcagct catttaaaac
      661 aaaaaccaat gagtcgtatg gctaaattgg aatatggcga taaataccaa accatcacac
      721 tattaccaaa gtcaggtagc gggtagacat ttaaaaaacc aaaacaacca gttaaattat
      781 cgttttcact tgcccgagtc accttggtta gatgttcaag tggcctatgc ggaaaatcaa
      841 ggcaaacaaa actacaacaa agggagctga attaataata aatatttaat tcaaggtttg
      901 gcaattaaca acaaagcgac aaccttaaat ttgagtaaca cctttaaagt gtctccgttt
      961 aaagatggag aatgggaagc aatattaggt tttaatcgtt taaagaaccg ctatagtaaa
     1021 aatagacatc ctgcggaatt aaactataac ttagagaatg gcgaaaccag cgaaaacaac
     1081 gattctgtag gaatgacagc cgttcgtaaa tcgctttata cttcaacttt ccagcctgat
     1141 ggcgaacaga agttcgatac tttttatctc gacaatcact ttaactatgg tatatgggga
     1201 ttggatataa acgcaaatat taataccgct cggtttattg gtcagcgttt taaatatttt
     1261 ccttttgcga ttagagaatt ggagtcagat attagacagg caagtagaaa taggcagttt
     1321 gagcatttaa ggtcattaca atctcaatta gacaacttgc aaaaaaacat tgtacatata
     1381 ttccagatga ggattttcct aatgatcctg aattgggaga gtttaactgt cctgatgtca
     1441 attttcctca tacagcaaca ggaaaacata aacgaattaa ttattcagca accttgtctg
     1501 cttatattca cgatttattc acgcccttta ttagttattc tcaaacacat cgagccccta
     1561 atattaaaga aatattcttc tctaatttag gtgactatgg tgtaaatacc gcactacaac
     1621 cagagcaagc gaaaacaaca cagtttggta ttaatggatt caaagaacgg gtatttagtg
     1681 aagaagataa attaggattt aaagtgctat tttataacac t
//
LOCUS       DANLYI010000278         1710 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.280, whole genome shotgun sequence.
ACCESSION   DANLYI010000278 DANLYI010000000
VERSION     DANLYI010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1710
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.280"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            complement(167..1669)
                     /gene="ppx"
                     /locus_tag="PXD81_002430"
     CDS             complement(167..1669)
                     /gene="ppx"
                     /locus_tag="PXD81_002430"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HDL5538408.1"
                     /translation="MSSNIAPREFAAIDLGSNSFHMIVARIVNGSIQVLSRLKRRVRL
                     ADGLDENLVLSQDAIDRGIACLALFAKRLEGFSAENVKVVGTYTLRRAINNQAFLEQA
                     KAVFPYPISIISGQQEARLIYSGVSHTQPETGRKLVVDIGGGSTEMTIGDDFTPIRAE
                     SRHMGCVSFAKRFFPQGELTIERFDSAYQLAMEKIEDLMWEYKELGWDYALGSSGTIK
                     TVHQVLIANGYRDGLITESRLQKIIDICLEFKSLNEIKLRGLLDERADVLVPGLAILL
                     ALFHTFRIDVLRYSNGALREGVMYGLEKSFQVGDIRQRTAEALAEQFALDKQQAKRVE
                     HTALILFDQIKTWKNRRQVQDLRSMLKWASLLHEIGIVVNHNNVHRHSAYLIANRDLP
                     GFDFEQQTLLSVLMRYHLKGFKRSDIRSINRYQCRDVLTLVRVFRLAVLLNRSRQATV
                     VPKQLNLVIENNAWQLTFEQGFLANNPLISIDLEEEQKILASTNLQFAVS"
BASE COUNT          539 a          387 c          296 g          488 t
ORIGIN      
        1 ggataattcg taaaatatat aatctcaata agcaaacatc aaaaatgaga agttgatcaa
       61 attttaaaaa atactttttg caaaaaatta gctaaatttg accgcttgtt cgcaaaaggg
      121 aatgcaataa acgaaaaagg aagccttcgc ttccttcatt taaatgttaa gagaccgcaa
      181 actgaagatt tgtactcgcc aatatttttt gctcttcttc taaatctatt gaaatcagtg
      241 gattattcgc taagaagccc tgttcaaagg taagttgcca agcattattt tcaatcacca
      301 aattcaactg tttcggcact acagtcgctt ggcgagaacg gtttaatagc accgctagcc
      361 ggaaaacccg taccaacgtt aatacatctc tacattggta acggttgata ctacgaatat
      421 ccgagcgttt gaatccttta aggtggtatc gcattaatac cgatagaagg gtttgctgtt
      481 caaaatcaaa tcccggtaaa tctcggtttg caatcaaata agcagaatga cgatgcacat
      541 tattgtggtt tacaacaata ccaatctcgt gtaataaact tgcccatttc agcattgaac
      601 gcagatcttg cacttggcgg cggttcttcc aagttttgat ttggtcaaat aaaatcaacg
      661 ccgtatgctc cacccttttt gcttgctgtt tatctaaagc aaattgctca gctaacgctt
      721 ctgcagtccg ttggcgaata tccccaactt ggaaactttt ctccaaacca tacattaccc
      781 cttctcttaa agccccgttg gaataacgta atacatcaat tctaaaagtg tgaaaaagtg
      841 ctaaaagaat agctaagccc ggaactagca catctgctcg ttcatcaagt aagcctcgta
      901 gcttaatctc atttaacgat ttaaactcta ggcaaatatc aataattttc tgtaggcggc
      961 tttctgtaat taagccatca cgatagccat tggcaatcag cacttgatgg acggttttaa
     1021 tcgtaccgga agaacctaaa gcatagtccc aacctaactc tttgtactcc cacattaaat
     1081 cttcgatttt ttccattgca agctgatagg cactatcaaa acgctcaatc gttaattccc
     1141 cctgcgggaa aaagcgtttt gcaaaactca cacaacccat atgacgactt tccgctcgaa
     1201 tcggggtaaa gtcatcacca atagtcattt ccgttgaacc accgccaata tctaccacta
     1261 attttctgcc agtttcgggt tgggtatggc taacgcccga ataaatcagc ctcgcctctt
     1321 gttgtccgga aataatggaa atcgggtaag gaaatactgc tttcgcttgc tccagaaaag
     1381 cttggttatt gattgctcta cgtaaggtat aagtgccaac caccttcaca ttctctgcag
     1441 agaacccttc taaacgtttt gcaaataaag ctaaacaagc aatgcctcta tcaatcgcat
     1501 cttggcttaa tactaagttt tcatcaagcc catctgccag ccgtacacgc cgtttcaaac
     1561 gggaaagcac ttgaatggaa ccatttacga tacgggctac aatcatatga aagctgttcg
     1621 agcctaaatc aatagctgca aactctctag gagcaatatt tgatgacata atgaaaccct
     1681 gtaccaataa ctgaaataag taagatatta
//
LOCUS       DANLYI010000279         1700 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.281, whole genome shotgun sequence.
ACCESSION   DANLYI010000279 DANLYI010000000
VERSION     DANLYI010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1700
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.281"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            <1..613
                     /locus_tag="PXD81_002431"
     CDS             <1..613
                     /locus_tag="PXD81_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5538409.1"
                     /translation="LAAISANLGKVTAGTITGTTISGGTISGTTITGGIIKGARIEGL
                     TGKFTGELEINQLIGGNIYEAKTWTSKATGKSKTYSETKNSFALSDTYYEYVCQFVLP
                     ATKATRTLYVVHTPTGESKSETFYGSGSNRTPTTITLPSVIDISNTISPNVSNPFNDS
                     GAILIANTQYVVTCRAWCAGRGRSAKVNFRAIISSTNGSSYQL"
     gene            623..781
                     /locus_tag="PXD81_002432"
     CDS             623..781
                     /locus_tag="PXD81_002432"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5538410.1"
                     /translation="MKKIILLTTIMVSACTTQYKQDYTTKPTFCYQLAPQEQLPGKNC
                     IGSGGHSG"
     gene            complement(790..1089)
                     /locus_tag="PXD81_002433"
     CDS             complement(790..1089)
                     /locus_tag="PXD81_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HDL5538411.1"
                     /translation="MKTLDQMLNELSPERRQKVEDMAQEMIREIQLSKLREELNLSQQ
                     QLATALGISQPAIAKLEQKDNDPRLSTLKRYIEAMGGKLKLAVELPTGDERVIRI"
     gene            complement(1061..1450)
                     /locus_tag="PXD81_002434"
     CDS             complement(1061..1450)
                     /locus_tag="PXD81_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDL5538412.1"
                     /translation="MADPIEWSIITTDCFDEWFGQQDGSTQERVLACLIYLRKFGYSL
                     GRPYADTVYQSKFPNMKELRIQNKGKPIRAFYAFDPLRQAIVLCAGEKGKDKRFYEKM
                     IALADAEFTSYLNNLEKENENVRSNAQ"
BASE COUNT          556 a          308 c          318 g          518 t
ORIGIN      
        1 tttagcggca atatcggcaa atcttggtaa ggtaactgct ggtacaatta cagggacgac
       61 aattagtggt gggacaattt caggtacaac aattacaggg ggaataatta aaggagcaag
      121 aattgaggga ttaacaggta aatttactgg agaacttgaa atcaaccaat tgatcggagg
      181 taatatctat gaggctaaaa cttggacatc aaaagcaaca gggaaaagta aaacatattc
      241 agaaacgaaa aacagttttg cattaagtga tacatattat gagtatgtat gtcaatttgt
      301 actacctgct acaaaagcga cgagaacatt gtatgttgtc cacactccaa caggagagag
      361 taaaagcgaa actttttatg gtagtggtag taatcggaca ccgacaacaa ttacattacc
      421 gtctgtaatt gatattagca atactatttc acctaatgta tctaacccat ttaatgattc
      481 tggggcaata ctcattgcta atactcaata tgtagtaacg tgtcgtgcgt ggtgtgcagg
      541 gcgtgggaga agtgctaaag tgaattttcg agcaattata tcatctacaa atggttcatc
      601 ttatcaatta taggaggcaa atatgaaaaa aattatacta cttacaacaa ttatggtttc
      661 agcttgtaca actcaatata agcaagacta tacaacaaaa ccaacctttt gttatcaact
      721 agcccctcaa gagcaattac cggggaaaaa ctgtattggc tcaggagggc attcaggtta
      781 acaaagtaat taaatcctaa ttacacgctc atcgcctgta ggcaattcaa cggcaagttt
      841 taacttaccg cccattgctt caatatagcg tttgagcgtg gataatcttg gatcgttatc
      901 tttctgttca agtttagcaa tagcaggctg tgaaataccg agagctgtag ctaattgttg
      961 ttgagagaga tttagctctt ctcttagttt tgaaagttgt atctctctaa tcatttcttg
     1021 agccatatct tccacttttt ggcggcgttc aggagagagt tcattgagca tttgatctaa
     1081 cgttttcatt ttctttctct aaattattta aataggaggt aaattccgca tcagcaagag
     1141 cgatcatttt ttcatagaat cttttgtctt tacctttctc gccagcacaa agtactatcg
     1201 cttggcgtaa agggtcaaaa gcataaaatg ctcgaattgg cttacctttg ttttgaatgc
     1261 gtaactcttt catattaggg aatttagact gataaactgt atcagcataa ggtctgccta
     1321 gactataacc gaatttccgc agatagatta aacaagccaa gacacgttct tgagtacttc
     1381 catcttgttg accgaaccat tcatcaaagc agtcagtcgt gataatagac cattctatag
     1441 ggtctgccat ttttgaacct tttataactt actatttata aatgaagtat aaataaaagg
     1501 ttatattatg taaagcaatt tactcttcct actaagccgt ttgtatgact acaagcggtc
     1561 tttttttatc aacaatccac aaaaggaaaa attatgacaa cttttaacaa aatcttaaac
     1621 ccaatgtatt ccaccatcgc aagctactca acgcaagatg acggctcact caatgctaag
     1681 tatgttgttg gcacaggtga
//
LOCUS       DANLYI010000280         1694 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.282, whole genome shotgun sequence.
ACCESSION   DANLYI010000280 DANLYI010000000
VERSION     DANLYI010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1694
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.282"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            82..1089
                     /gene="rfbB"
                     /locus_tag="PXD81_002435"
     CDS             82..1089
                     /gene="rfbB"
                     /locus_tag="PXD81_002435"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HDL5538413.1"
                     /translation="MIILITGGAGFIGSALVRYLIEHTPHTVINVDKLTYAGNLASLE
                     SVAHHERYHFEKADICASGQISEIFGKYQPDVVMHLAAESHVDRSISGAADFIQTNIV
                     GTYTLLEAARAYWDTLPEPRKSAFRFLHISTDEVYGDLAKSEELFTEKTPYNPSSPYS
                     ASKAASDHLARAWHRTYGLPTVITNCSNNYGAYQYPEKLIPLMILNALEGKSLPVYGN
                     GLQVRDWLFVEDHIRALYLVLQKGQIGETYNIGGHNEKTNIEVVRSICSILQEMVPNN
                     PLVVGQYADLITYVQDRPGHDFRYAIDTSKIEKELDWKPKETFESGLRKTVEWYLANR
                     N"
     gene            complement(1191..>1694)
                     /locus_tag="PXD81_002436"
     CDS             complement(1191..>1694)
                     /locus_tag="PXD81_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HDL5538414.1"
                     /translation="IQKRYNAAKNDIIVFLEKGNRHSSLGARFDMWENALIGIREKPL
                     LGHGSTGYETFKKKQIETKQMAKTTLRFNSLHNQYLEAFVKRGVVGFMALMAILIIPF
                     VIFAKRLKAKNREVKCMAILGIVHIISHSFFFLSQSFLAHNSGSLFYFFVLILLYHLV
                     KQNEEII"
BASE COUNT          517 a          333 c          325 g          519 t
ORIGIN      
        1 taaaaaagac gctgaaaaga atattcaaag ccatattaaa tacaaacgaa atttcgtgaa
       61 tcaaatttca tattcacctt tatgatcatc ctcatcactg gcggagccgg ttttatcgga
      121 tctgcattag ttcgctatct gattgaacat actccacaca cagttatcaa tgtggataag
      181 ctcacctatg cgggaaattt agcctcattg gagagtgtcg cacatcacga acgctaccat
      241 tttgaaaaag cagatatttg tgcaagcggt caaatttctg agatttttgg caaatatcag
      301 cctgatgtgg taatgcactt agcagcggaa agccatgttg atcgctctat ttctggggca
      361 gcagatttta ttcaaaccaa tattgtcggc acttatactt tattagaggc agcgagagcc
      421 tattgggaca cgttacctga gccaaggaaa tcggcgtttc gctttctaca tatctcgacc
      481 gacgaggtct atggtgattt agccaaaagc gaggaattat ttaccgagaa aaccccttat
      541 aatccaagta gcccctattc ggcatctaaa gctgccagcg atcatctggc tcgggcttgg
      601 catcgcacct acggtttacc gactgtcatc accaactgtt ccaataatta tggggcatac
      661 caatatcccg aaaaacttat tccgttaatg attttaaatg ccttagaagg taaatcccta
      721 cctgtttacg gcaacggctt gcaggtcaga gattggctgt ttgtggaaga tcatattcgt
      781 gccctctatt tggtgttgca aaaagggcaa atcggagaaa cctacaatat tggcgggcat
      841 aatgaaaaaa ccaatatcga agtggttcgt tctatctgtt ctattttgca agaaatggtg
      901 ccaaataacc cgcttgttgt aggacaatat gctgatttaa ttacttatgt gcaagatcgc
      961 cctggccacg atttccgcta tgcaattgat acttcaaaaa tcgaaaagga actcgactgg
     1021 aaaccgaaag aaacttttga aagtggcttg cgtaaaacag tggaatggta tttggctaat
     1081 cggaattagt agtaagaagc agaaaaagag gaaatatccc gacatttatc ctgtggtaga
     1141 agatgaagtt accacctcgg ttatattccc aactttcttc tattgtttta tcatattatc
     1201 tcttcgttct gtttgactaa atggtagagt aaaattaaca caaagaaata gaataatgag
     1261 cctgaattat gggctaaaaa tgattggctt aggaaaaaga atgaatgcga aataatatgt
     1321 acaatgccta atatcgccat acattttacc tctctatttt ttgcctttaa tcgtttggca
     1381 aaaataacaa atggaataat taaaatagcc attagcgcca taaaaccaac tacacctctt
     1441 ttcacaaagg cttctaggta ttggttgtgt aagctattaa acctaagcgt tgtttttgcc
     1501 atttgttttg tttcaatctg tttctttttg aatgtttcat agcctgtgct accgtgtcct
     1561 aatagcggtt tttcacggat tccgataagc gcattttccc acatatcaaa acgagctccc
     1621 agagatgaat gtctatttcc tttttctaaa aatacgataa tatcattctt agctgcatta
     1681 tatcgttttt gtat
//
LOCUS       DANLYI010000281         1690 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.283, whole genome shotgun sequence.
ACCESSION   DANLYI010000281 DANLYI010000000
VERSION     DANLYI010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1690
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.283"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            complement(302..994)
                     /locus_tag="PXD81_002437"
     CDS             complement(302..994)
                     /locus_tag="PXD81_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HDL5538415.1"
                     /translation="MTELTPKQEAFCFAYIETGNASEAYRQAYETEDMKSETVHRKAK
                     ELMDNGKITARIEELKAEHAERHKLTVDDLLKELEEARILAREKENPNAMTQATMGKA
                     KLLGLDKQVTDPCFDGETGKGDMILSVNCSSIKDGKALDETCILKAGTHEFITRDSFI
                     FYRHIRIDSLDEIKANIEAGYFIQKALINDEAYQQILKGVLSSKSVTPRYKRFLKNAI
                     RQSACPDILAEP"
     gene            complement(991..1416)
                     /locus_tag="PXD81_002438"
     CDS             complement(991..1416)
                     /locus_tag="PXD81_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5538416.1"
                     /translation="MKHELNLAGITALMNEPIKATLSFLDFPHIRIERLHHDNGRPFF
                     IGVLSLDLEDHINIEMAGATGFLEALAIIAYHQQLTATPLLVNDDLLSEINKLPHPQR
                     TNPFIDRHGNEITREQMSKEYREKIDYAFAEKRMAEGKE"
     gene            complement(1423..1575)
                     /locus_tag="PXD81_002439"
     CDS             complement(1423..1575)
                     /locus_tag="PXD81_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5538417.1"
                     /translation="MPELLIVLGNRIAKLEQQHNAMPSETDSITLGILRKQLSQLLKV
                     AERIKE"
BASE COUNT          408 a          328 c          362 g          592 t
ORIGIN      
        1 aggctatttt gtgtacaatt attcggatcg tgtataggga aatttagttc gtagttttct
       61 atagtagttt aagtttataa agaaaaaatt taaaactttt tgaaatttta cccactttac
      121 ccactgattt gttggaagcc ttgatatata aggctttata gccagtggga agcaagtggg
      181 ttacagtggg ttaagtgggt aactttgatt tttacccact gttagccact tttagagggg
      241 aaatattagc ggttttggta gtggttaaag ggcaaaaaag gccttaattc tcatcaacgg
      301 cttaaggttc ggctagaata tcagggcaag cactttggcg gattgcattt tttaggaagc
      361 gtttatatct aggcgttact gattttgacg atagtacgcc ttttagtatt tgttgatagg
      421 cttcatcatt aattagggct ttttgaatga aataaccggc ttctatattt gccttaatct
      481 catctaacga atcgattctt atatggcggt agaaaataaa gctgtcccgt gtgataaatt
      541 cgtgtgttcc tgcctttaaa atacaagttt catcaagtgc tttcccgtct ttaatgcttg
      601 agcagtttac acttaaaatc atatctcctt taccggtttc accatcaaag cacggatcgg
      661 taacctgctt atccagccct agcaacttcg ctttgcccat tgtggcttga gtcattgcgt
      721 tagggttttc tttctctctc gccagtatac gagcctcttc cagctccttt aaaaggtcat
      781 caacggttaa tttatggcgt tctgcgtgtt cggctttcag ttcctcaatc cttgccgtaa
      841 tcttgccgtt atccattagc tctttagctt ttcggtggac ggtttcggac ttcatatctt
      901 cggtttcata ggcttggcgg taggcttcgc tagcattgcc ggtttcaata taagcaaagc
      961 aaaaagcctc ttgttttggt gtgagttcgg tcattctttc ccctccgcca ttcgcttttc
     1021 agcaaaggca taatcgattt tttctcggta ttctttgctc atctgctccc gtgtaatttc
     1081 gttgccgtgt cggtcaataa atgggtttgt tcgttgtggg tgggggagct tgttgatttc
     1141 actcaataag tcatcattca ctaatagcgg cgtggcggtt agctgttgat gataggcaat
     1201 aatcgctaga gcctctaaaa agcccgtagc ccctgccatt tcaatattga tatgatcttc
     1261 aaggtcaaga gaaagcacac cgataaaaaa cggtctgccg ttatcgtggt gcagtcgttc
     1321 aattcggata tgcggaaaat ctaaaaagct caaggttgct ttgataggtt cattcattaa
     1381 agcagtaata cccgctaaat taagctcgtg tttcattttg gtttattcct ttattctttc
     1441 cgccactttt aacagttggc taagttgttt tcttaaaatg ccgagtgtga ttgaatcggt
     1501 ttcgcttggc attgcgttat gttgttgttc cagcttcgct attcggttgc ctagcacaat
     1561 taaaagctcg ggcaatgggt aagctcggtt tcagaataga ggcttaaaaa cgtgaatagt
     1621 ctgctaaata atcaggtgtt atctcagaaa aatcgagctt tttccctagt gctgcttcta
     1681 attcttgttt
//
LOCUS       DANLYI010000282         1677 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.284, whole genome shotgun sequence.
ACCESSION   DANLYI010000282 DANLYI010000000
VERSION     DANLYI010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1677
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.284"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            154..1125
                     /locus_tag="PXD81_002440"
     CDS             154..1125
                     /locus_tag="PXD81_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element ISApl1 family transposase"
                     /protein_id="HDL5538418.1"
                     /translation="MSTSYRHLTINEREKIMILLAQGKKQAEIAKALGRSSSTISREL
                     KRHALESYSATNAQNSYLKHRQNSKAQRKLEQPEYFNLVQEKFLTENWSPEQISARLK
                     LEKSELSISYSTIYRGIYSGLFDIGERKASRKLRHKGKTRHTKNHHEKRGKIQISNHL
                     NDRPISAQNRSRFGHWKADTVLGKAGGACLLTLTERKSRFELVKKIPAKKAEAVQKAM
                     IELLDSHILRSITPDRGKEFAQHRLVTEALGVEFYFPEPHQPWTRGTNENTNGLLREY
                     FPKHQDINQWSEVDIQQVVNKLNLRPRKCLGWKTPYEVYFKKTLHLV"
     gene            complement(1398..>1677)
                     /locus_tag="PXD81_002441"
     CDS             complement(1398..>1677)
                     /locus_tag="PXD81_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012340803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDL5538419.1"
                     /translation="QLFNINRFSLVTTSRNKKMTEGQLVHFFRHISEFIGFTVTSHRF
                     RHTTATEVLKQSGDIYAVKQLLGHKDLTVTLTYIHNDPEMLRKHVNSL"
BASE COUNT          570 a          320 c          321 g          466 t
ORIGIN      
        1 ctcccacttt ttttctataa ataaataatt tctgttttat cttctaaatg aactgatttt
       61 atacgctgaa tttacaatcc aagtgcaaca aaaaaagaag tactcatcaa ctagaatata
      121 attttgtttc cacacaaaaa ccacttccaa atgatgagta cttcctaccg acatcttaca
      181 ataaacgagc gagaaaagat aatgatttta ctcgcacagg gcaaaaaaca agcagaaatt
      241 gccaaagcac tgggacgtag ctccagcacc atttctcgcg agctgaaacg acacgctcta
      301 gaaagctaca gtgcaacgaa cgcacaaaac agctatttga agcatcgtca aaatagcaaa
      361 gcacagcgca aattagagca gcctgaatat ttcaatttgg tgcaagaaaa gtttctgaca
      421 gaaaactggt cgcccgaaca aatcagcgca cgattaaaat tggaaaaatc tgaattatcc
      481 attagttatt caaccattta tcgtggtatt tattcagggt tgtttgatat aggcgaacgc
      541 aaagccagtc gcaaactgcg ccacaaaggc aaaacacggc atacaaaaaa tcatcatgaa
      601 aaacgtggca aaattcagat atccaaccat ttgaacgacc gtcccatttc ggcgcaaaat
      661 cgcagtcgct ttggacattg gaaagccgat accgtactgg gtaaagcggg tggagcttgt
      721 ttgctgacgc tgacggaacg caaaagtcgt tttgagttgg tgaagaaaat tcctgccaaa
      781 aaagccgaag cagtccaaaa agccatgatt gaattgctgg attcacatat attgcggtca
      841 attacgccag accgtggtaa agaatttgcc caacatcgtt tggtaacaga agcactgggt
      901 gtagaatttt acttccccga gccgcatcaa ccgtggacac ggggaacgaa tgaaaataca
      961 aatgggttac ttcgtgaata ctttccgaag caccaagaca tcaatcagtg gagcgaagtt
     1021 gatattcaac aggtggtcaa taaactgaat ttacgaccgc gtaaatgttt gggttggaaa
     1081 acaccttatg aagtttactt caaaaaaacg ttgcacttgg tttgacaatt caagacaata
     1141 attttgtcac ccttaggaaa attattcatg gtcctaagat tcagtaaaga ttgtggaata
     1201 aataagtgat cgtcatttat caatgtgaga gcaaagactc tggttttagt tctaagagct
     1261 ttatcaaaag aaaggtttaa ataaattgca cccgatgaca aagttatctc tggattaatt
     1321 aaaaaatcgg ggctgacgta gactagcata cttactaatc tacgtcagtc ccttatttta
     1381 tctatgtagc atcatttcta caacgaattg acatgttttc ttagcatttc aggatcattg
     1441 tgaatatatg ttagagttac agtgagatct ttatgtccaa gtaattgctt cactgcatag
     1501 atatcccctg attgctttag gacttctgtt gcagttgtat gtcggaatcg atgtgaagtt
     1561 actgtaaatc ctataaattc agaaatatgg cgaaaaaaat gaactagttg tccttctgtc
     1621 attttcttat ttcttgatgt agtaactaat gaaaagcgat taatgttaaa taactgc
//
LOCUS       DANLYI010000283         1676 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.285, whole genome shotgun sequence.
ACCESSION   DANLYI010000283 DANLYI010000000
VERSION     DANLYI010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1676)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1676)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1676
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.285"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            <1..821
                     /locus_tag="PXD81_002442"
     CDS             <1..821
                     /locus_tag="PXD81_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HDL5538420.1"
                     /translation="QKIYQDKQSYLVEIEKSMNDYAELVKRYEKLLKVGHSSHDEVNI
                     QKSRYFQQKSLFNELKQELIQLKSTQLSLENEIETRKTEFNNQIIRYEMQKSDLNIRL
                     MEFESVSDIIVNASLDGKIESTSVTVGQIIKENDPLAQILPQNKGNYQLVMWVPNSAI
                     SFIKQGDEVNIRYEAFPFEKFGQFKGKITSISTLPASLQELSFYKNLPVNLEQGIPLY
                     KILIELADQNVKYNDTSLYFMSGMKAEATLFLEKRKLYEWIFFPMYQLRKNMEQ"
     gene            821..1612
                     /locus_tag="PXD81_002443"
     CDS             821..1612
                     /locus_tag="PXD81_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C39"
                     /protein_id="HDL5538421.1"
                     /translation="MTGIKQLKFDFRRRIPVILQTETAECGLACLAMMLGYYDKNTNL
                     FELRSQYGTSSRGVTLHTLISIANDCGLITRPLSLELDEVPQLRLPCILHWDFNHFVV
                     LTHASEKQFIIHDPAFGKRKLSRAEFSNHFTGVALEVWSEVKFEKSNNENTISLYETL
                     KHISGIKGALVKIFALSMLIELIALLMPIGTQLVMDHVMQAKDQSLLLIICIGLFLFT
                     LFRTMVSMFRAWVSLKMNYLIDFQWTASFFSHLLKLPLDFFENAK"
BASE COUNT          556 a          274 c          295 g          551 t
ORIGIN      
        1 gtcaaaaaat ttatcaagat aagcagagtt atttagtgga aattgaaaaa agtatgaatg
       61 actatgcgga gttggttaaa cgctatgaga aattattaaa agtagggcat tccagccacg
      121 atgaagtgaa tattcagaaa agccgttatt tccaacaaaa atcactgttt aatgagctaa
      181 agcaagaatt aattcaatta aaatccaccc aacttagctt ggaaaatgaa attgaaaccc
      241 gtaaaaccga atttaataat cagattattc gctatgaaat gcaaaaaagc gatttaaaca
      301 ttcgtttaat ggaatttgaa tctgtttcag atattattgt taatgcctct ttagatggaa
      361 aaattgaaag taccagcgta acagttgggc aaattattaa agaaaatgac ccgcttgcac
      421 aaattctgcc acagaataaa ggaaattatc agctagtgat gtgggtaccg aatagtgcca
      481 tttcatttat taaacaaggc gatgaggtga atattcgcta tgaggctttt ccgtttgaaa
      541 aattcgggca gtttaagggc aaaattacgt caatttcaac cttaccagct tctttgcaag
      601 aacttagttt ttataaaaat ctccctgtaa atttagagca aggtattccg ctttataaaa
      661 ttctgattga gcttgccgat caaaacgtaa aatataatga tacctccctt tattttatga
      721 gtggaatgaa agccgaagcc acgttatttt tagaaaaacg taaactgtat gagtggatat
      781 ttttccctat gtatcaatta cgcaaaaata tggagcaata atgacaggaa ttaagcagtt
      841 aaaatttgat tttcggagaa gaatacctgt tattttgcaa actgaaacag ctgaatgtgg
      901 cttagcctgt ttagcgatga tgttgggcta ttacgataaa aacaccaatt tatttgaatt
      961 gcgaagtcaa tatggtactt cctctcgtgg cgtaacgctg catactttaa tttctattgc
     1021 aaatgactgt ggtttaataa ctcgccctct ttctctagag ttagatgaag taccacaact
     1081 tcgtctgcct tgtattcttc attgggattt taatcatttt gtagtattaa ctcacgcaag
     1141 tgaaaaacag tttattatcc acgatcctgc tttcggtaaa cgtaaattat cccgtgctga
     1201 attttccaac cattttaccg gagtcgcttt agaagtttgg tctgaagtta aatttgagaa
     1261 aagcaacaat gaaaatacca ttagtttgta tgaaacctta aagcatattt ctggcataaa
     1321 aggtgcatta gtgaaaattt ttgccctttc aatgctcatt gaactcattg ccttattgat
     1381 gccaattgga acacagttag tgatggatca cgttatgcaa gcgaaagatc aatcattact
     1441 tttaattatc tgtattgggc tatttttatt tacccttttt cgcacaatgg tcagtatgtt
     1501 tagggcttgg gtttccttga aaatgaatta tttaattgat tttcaatgga cagccagttt
     1561 cttttctcat ctcttaaaat taccgcttga tttctttgaa aacgccaagt aggtgatatt
     1621 cattcccgat ttagttcatt aaaaaccatt cagaaaactc taacttcaag cattat
//
LOCUS       DANLYI010000284         1645 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.286, whole genome shotgun sequence.
ACCESSION   DANLYI010000284 DANLYI010000000
VERSION     DANLYI010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1645
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.286"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            complement(<1..478)
                     /locus_tag="PXD81_002444"
     CDS             complement(<1..478)
                     /locus_tag="PXD81_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005595838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5538422.1"
                     /translation="MADPHIQSPMDFWDYLTVIIYRSGFVLATLMLLLLPYYPQTAQY
                     GLLVSGTMLASSLHLYAKSFRLIFQFSVWIGLLGAVFNFPLLAFGAMLLVIGGLSYKE
                     YFCFRVFGLNFQPLLVAVLWIAFALEWSLLVQILSLVCGLLLLVLSIQKWRMPLHFD"
     gene            complement(501..1478)
                     /gene="dusA"
                     /locus_tag="PXD81_002445"
     CDS             complement(501..1478)
                     /gene="dusA"
                     /locus_tag="PXD81_002445"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HDL5538423.1"
                     /translation="MTNEIQPFYRGRFSVAPMLDWTTRHCRYFHRQFSRQALLYTEMI
                     TAPAIIHAKYDLLEYDPSENPVALQLGGSDPAQLAHCAKLVAERGYAEINLNVGCPSD
                     RVQNGMFGACLMAKADLVADCIKAMQDAVEIPVTVKHRIGIDDLDSYEFLCDFIEKVQ
                     PYSNDFIVHARKAWLTGLSPKENREIPPLDYDRVYQLKRDFPHLKISINGGIKTIEEI
                     KQHLQFVDGVMVGREAYQNPSMLGEIDSQIFNENRPLVTAKEAVEKMFPYIERELAKG
                     VYLNHIVRHMLGAFQHCKGARQWRRHLSENATKSGAGVEVVEQALAFIG"
BASE COUNT          544 a          397 c          295 g          409 t
ORIGIN      
        1 tatcaaaatg cagcggcatt cgccattttt gaatactcag cacaagaagc aacaagccac
       61 agacaaggct taaaatttgc accaataaag accattctag tgcaaaagca atccacaata
      121 ccgccactaa aagcggttgg aaatttaagc cgaatactct aaaacagaaa tattctttat
      181 agcttaatcc gccgatcacc agtagcattg ccccgaacgc aagcaaaggg aaattaaaca
      241 cagcaccaag taagccaatc caaacagaaa attggaaaat caggcgaaac gatttggcat
      301 aaagatgcaa agaagatgcc aacattgtgc cagatactaa taaaccatat tgtgccgttt
      361 gcggataata aggcaataaa agcaacatca atgtagctaa cacaaaaccg ctgcggtaaa
      421 tgatgacggt taaataatcc caaaaatcca ttggcgattg aatatgtgga tctgccataa
      481 ctgcctcctt aaacaataaa ttagccaata aacgcaaggg cttgttccac tacttctaca
      541 ccggctcccg acttagtggc attttcactt aaatgacgac gccactgcct cgctcctttg
      601 caatgttgaa acgcaccaag catatggcga acgatatgat ttaaatagac acctttggca
      661 agctcacgtt caatataagg gaacatcttt tccaccgctt cttttgccgt tacaagcggt
      721 cgattttcat taaaaatttg cgaatcgatc tcgcctagca ttgacgggtt ttgataggcc
      781 tcacgcccta ccattacccc atcaacaaac tgcaaatgct gtttaatttc ctcaatggtt
      841 ttaatgccgc cattgatgct aatctttaaa tgcggaaaat ctcgcttgag ctgataaacc
      901 cgatcataat ctaaaggcgg aatttcacgg ttttccttcg ggcttaaccc tgtcagccac
      961 gccttgcggg cgtgtacgat aaaatcattg ctataaggtt gcactttttc gataaaatca
     1021 cacaaaaatt catagctgtc taaatcatca atcccaatac gatgtttaac cgtgaccgga
     1081 atttccaccg catcttgcat tgctttaata caatccgcca ctaaatctgc ttttgccatc
     1141 aaacaagccc caaacatacc gttttgcact cggtctgacg ggcaaccgac atttagatta
     1201 atctcggcat agcctcgctc tgccaccaat ttggcacaat gagccaactg tgccgggtcg
     1261 cttccaccca gttgcaaagc caccggattc tcgctcggat catattccaa caaatcatat
     1321 ttggcgtgaa taatcgccgg ggcggtgatc atttcggtat aaagcaaggc ttgacgacta
     1381 aattggcgat gaaaatagcg gcagtgacgg gttgtccaat ctaacattgg agcaaccgaa
     1441 aaacgtccac ggtaaaaagg ttgaatttcg ttagtcataa aaatatcaaa ataaagcggt
     1501 aaaaaatcag taaaaaataa ccgcttgtaa gcgattcaaa acaagcagcc atcatactcg
     1561 aaaaagccag ataaacatag tttcctcaag gcttccccat ctatctgcgc acttttaaaa
     1621 caaccttaaa acacaatata tagtg
//
LOCUS       DANLYI010000285         1645 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.287, whole genome shotgun sequence.
ACCESSION   DANLYI010000285 DANLYI010000000
VERSION     DANLYI010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1645
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.287"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            <1..1642
                     /locus_tag="PXD81_002446"
     CDS             <1..1642
                     /locus_tag="PXD81_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HDL5538424.1"
                     /translation="DLNADTSQAFLNELDQTLWTAADKLRKNLDAANYKHIVLGFIFL
                     KYISDSFTDFQAKLKTQLTTPESELYLDPALFDEQEFSQILAEELEQRDYYAAENIFW
                     VPEQARWDNIKSLSKLNLGDELPWGDKFKGVSRLIDDAFEAIERENPKLKGVLQRIAG
                     FGVPDEMLTGLIDLFSRTNFTQPMHNGEPVHLQAKDILGHVYEYFLGQFALAEGKKGG
                     QYFTPKSIVTLIVEMLEPYSGRIYDPAMGSGGFFVQADRFIQAHAGNRNAISVYGQES
                     NSTTRKLAVMNMAIRGIPFDFGDKPEDTLLNPLHIDKKMDVVMANPPFNQKEWWNESL
                     ANDPRWAYGTPPQGNANFAWLQHMIYHLSPKGKMALLLANGSMSSQTSGEGDIRKNIV
                     QADLVEAMIALPNQLFTNTQIPACIWIINKAKARKGEVLFINATQIGYLKDRVLRDFT
                     ADDIAKISDTYHNWQKQNGYENIPAFCYCATLDEIAKNDFVLTAGRYVGAVQEENDGV
                     PFAEKMQELTALLNEQFKQGRELEQQIAENLKGLGYGI"
BASE COUNT          486 a          437 c          356 g          366 t
ORIGIN      
        1 tgatctcaac gctgacacaa gtcaagcctt tttaaacgaa ctcgaccaaa ccctctggac
       61 tgccgccgac aaactgcgta aaaacctcga tgccgccaac tacaaacaca tcgttcttgg
      121 ctttatcttc ctaaaataca tctccgacag ctttaccgat ttccaagcca agctaaaaac
      181 ccagcttacc acccccgaaa gcgaactcta tcttgaccct gcactatttg acgaacaaga
      241 atttagccaa attcttgccg aagagttgga acagagagat tactacgccg ctgaaaacat
      301 cttttgggtg ccggagcaag cccgctggga caacatcaaa tcattaagca aactcaatct
      361 tggcgatgaa ttgccttggg gagacaaatt taaaggtgtc agccgcttga ttgatgatgc
      421 ctttgaagcc atcgaacggg aaaaccccaa actcaaaggc gtactccaac gcattgccgg
      481 ctttggcgtg cctgatgaaa tgctcacagg cttaattgac ctgttctcac gcaccaattt
      541 cacccagcct atgcacaatg gcgaacctgt gcatctgcaa gccaaagaca ttttagggca
      601 cgtctatgaa tactttcttg ggcaatttgc ccttgccgaa ggcaaaaaag gcggtcaata
      661 cttcacgcca aaatccatcg ttaccctgat tgttgaaatg ctcgaaccct attcagggcg
      721 gatttacgac ccagctatgg gcagcggcgg cttttttgtg caagctgacc gctttattca
      781 ggctcacgca ggcaaccgca acgccatttc cgtttatggg caagaatcca actccaccac
      841 tcgcaaactg gcggtgatga atatggcgat tcgtggtatt ccctttgact ttggcgacaa
      901 gcccgaagat accctactaa accctttgca catcgacaaa aaaatggatg ttgtgatggc
      961 aaatccgccc tttaaccaaa aagagtggtg gaatgaaagc ctagcaaacg atccacgctg
     1021 ggcatacggc acaccgccgc aaggcaacgc caactttgcg tggttgcaac atatgattta
     1081 ccacctctcc cccaaaggca aaatggcact cctgctcgcc aacggctcaa tgagcagcca
     1141 aacttcaggc gaaggcgaca ttcgcaaaaa catcgtgcaa gctgaccttg tcgaagcgat
     1201 gattgccctg cccaatcagc tattcaccaa cacccaaatc cctgcctgca tttggattat
     1261 caataaagcc aaagccagaa aaggtgaagt gctgtttatc aacgccaccc aaataggcta
     1321 cctgaaggac cgcgtcttgc gtgattttac cgctgatgac atcgccaaaa tcagcgacac
     1381 ctaccacaac tggcaaaaac agaacggcta cgaaaatatc cctgcgtttt gttattgtgc
     1441 cacgctggac gaaatcgcca aaaacgattt tgtgctgacc gcagggcgat atgtcggtgc
     1501 ggtacaagaa gaaaatgacg gcgtgccgtt tgcagaaaaa atgcaggaat tgaccgcttt
     1561 attgaatgaa caatttaaac aagggcggga attggaacag caaatcgcag agaatttaaa
     1621 ggggttggga tatggcattt aatca
//
LOCUS       DANLYI010000286         1636 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.288, whole genome shotgun sequence.
ACCESSION   DANLYI010000286 DANLYI010000000
VERSION     DANLYI010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1636
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.288"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            27..980
                     /gene="msrP"
                     /locus_tag="PXD81_002447"
     CDS             27..980
                     /gene="msrP"
                     /locus_tag="PXD81_002447"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011609172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HDL5538425.1"
                     /translation="MKKLTIQDVTPETIFYQRRKIITALGLGIAASALPKVSMAETQR
                     HLQSLQFKPDPNKGLELTPENKVIGYNNFYEFGVDKSAPAKNAANFKTDPWTLEIGGE
                     VENPFSLNHQQLLNTFPLEERIYRFRCVEAWSMVVPWIGFELARLIEMAKPTSKAKYV
                     VFHTLYDPAQMPGQKNRLLGGGIDYPYVEALRLDEALNSLTLLSVGLYGKTLPPQNGA
                     PIRLVVPWKYGFKSIKSIVKITFAEKRPLTTWESLAPNEYGFYANVNPNVDHPRWSQA
                     SERVIGAGGLLSVKRQPTLMFNGYENEVANLYRGLDLKVNY"
     gene            980..1564
                     /locus_tag="PXD81_002448"
     CDS             980..1564
                     /locus_tag="PXD81_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoxide reductase heme-binding subunit YedZ"
                     /protein_id="HDL5538426.1"
                     /translation="MLTWLRILIHLGCLLPLVWVAQLLYTGNETILGADPIKELEHFL
                     GYGAIVIFCIMFLLGIALQYLKKNQYQILRRPLGLWAFVWAALHISSYLLLELSLDVK
                     LFFSELAGRPYLILGAAAFFILSIMAITSLPMLKRKLGTRWGTIHQWAYPALVLAMVH
                     YYWSVKSVTFDPIIIGVLVLFIVAHKIQAKFAKK"
BASE COUNT          508 a          317 c          330 g          481 t
ORIGIN      
        1 acaattcata aaaaaaggat agttcgatga aaaaattaac aattcaagat gttacacccg
       61 aaactatttt ctaccaacgc cgtaaaatta ttaccgcgtt aggtttaggt attgcagctt
      121 ctgcattgcc taaagtaagt atggcagaaa cacaaagaca cttgcaatca ctacagttta
      181 aaccggatcc aaataaaggg cttgaactca ctccggaaaa caaagtaata ggctataaca
      241 atttctatga gtttggcgtg gataaaagtg caccagcaaa aaatgcggca aacttcaaaa
      301 ccgatccttg gacattggaa attggcggag aagttgaaaa tccgtttagc ttaaatcatc
      361 aacaattgct taatacgttt ccactagaag aacgcattta ccgttttcgc tgtgttgaag
      421 cgtggtcaat ggttgtgccg tggatcggct ttgagttagc tcgcttaatt gagatggcaa
      481 aaccaaccag taaagccaaa tatgtggtct ttcacacttt atatgatcct gcacaaatgc
      541 cggggcaaaa aaatcgcttg ctaggcggtg ggattgatta tccttatgta gaagctttac
      601 gcttagatga agccttaaat tcattaacac tactatccgt cggtttatac ggtaaaaccc
      661 tgccaccgca aaacggtgct cctattcgct tagtcgtgcc ttggaaatat ggctttaaaa
      721 gtattaagtc gattgtgaaa atcacctttg ctgaaaaacg cccactcact acttgggaaa
      781 gtcttgctcc aaatgaatat ggcttttatg ctaatgtgaa tccaaatgtt gatcatccac
      841 gttggtcgca agcatctgaa cgagtaattg gtgcaggcgg gttattatcc gtcaaacgcc
      901 aaccaacact aatgtttaat ggctacgaaa atgaagtggc aaatttatac cgtggcttag
      961 atttaaaggt gaactactaa tgctaacctg gttacgtata ctcattcatc ttggctgttt
     1021 attaccgcta gtttgggtag cacagctatt atatacgggt aatgaaacaa tattaggtgc
     1081 cgatccgatt aaagagctgg agcattttct cggttatggt gcaatcgtca ttttttgtat
     1141 aatgtttcta cttggtattg ccctacaata cctaaagaaa aaccagtatc aaattttacg
     1201 ccgtccattg ggcttatggg cgtttgtatg ggcagctcta catatttcaa gctatctatt
     1261 gttagaatta agccttgatg taaaattatt ttttagcgag cttgccggtc gcccatattt
     1321 gattttagga gcagctgctt ttttcattct aagcataatg gcaataactt cgctgcctat
     1381 gctaaaacga aaattaggaa cacgctgggg gacgattcac cagtgggctt atcctgcatt
     1441 agtattggcg atggtacatt attattggtc ggtaaagagc gtaacattcg atccaattat
     1501 catcggagtc ttagtgctgt ttattgttgc tcataaaata caggctaaat ttgcaaaaaa
     1561 atagcaaaat cacaccgctt gcagcagtca aactacatac aaaaaataag gggctgtcct
     1621 agataacgac taaaac
//
LOCUS       DANLYI010000287         1635 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.289, whole genome shotgun sequence.
ACCESSION   DANLYI010000287 DANLYI010000000
VERSION     DANLYI010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1635
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.289"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            <1..218
                     /locus_tag="PXD81_002449"
     CDS             <1..218
                     /locus_tag="PXD81_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5538427.1"
                     /translation="KNLTDESEEQAILAYLTENIMARIEKEQPLEKTLILYSEQLASF
                     EQKSPMALHREKFEAKPTGLAELINNL"
     gene            229..1179
                     /locus_tag="PXD81_002450"
     CDS             229..1179
                     /locus_tag="PXD81_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P2 family phage major capsid protein"
                     /protein_id="HDL5538428.1"
                     /translation="MSLSTFAHALKLDKQGNVILFDDSPEEQAKLISRLKQNRLFKLL
                     NLVQTKHAKGEAIGLFTPIAGTTNTESGEEREPKQALAPAQKYHCEQINIDSYIRYEK
                     IDAYNLENSLEERLNQLLDDNLLRGLLMVGWQGVSRADTSNATNNQLAQDVKKGWLQK
                     IREGKPEAVINGATIGKEQQYKSLNALIKQGLGKISPAYAMGGDMIAICGRDIIADDV
                     IQLEHNDLSENLADLLTLCQQAKGGLKAVSIPYFPSNAILITRLDNLSLYLHLNNIRR
                     KAFDTPQKDSVETFYSLNVDYIVEDFNACCLIENIEVIEE"
     gene            1176..1370
                     /locus_tag="PXD81_002451"
     CDS             1176..1370
                     /locus_tag="PXD81_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5538429.1"
                     /translation="MSLESYIQQAEEMELLEKLDFVSDLLELEKVSEIEAYRILTAHG
                     EPKPQPAQEKLIGCVDFLDD"
BASE COUNT          611 a          329 c          309 g          386 t
ORIGIN      
        1 ataaaaacct cacagatgaa agtgaagagc aagctattct ggcatatcta acagaaaata
       61 taatggctag aattgaaaag gaacagccac ttgaaaaaac attaattctt tattctgaac
      121 agttagcaag ttttgaacaa aaatcgccaa tggcattaca ccgtgaaaaa tttgaagcaa
      181 aacctaccgg cttagctgaa ttaattaata acttataaga ggtaaaaaat gagtttatcc
      241 actttcgccc acgcattaaa actagataaa caaggtaacg ttattttatt tgacgatagc
      301 cccgaagaac aggcaaaact aattagccgc ttaaaacaaa atcgcctttt taaattattg
      361 aatttagtgc aaaccaagca cgcaaagggc gaagcgattg ggctattcac accgatagcc
      421 ggcacaacca acaccgaaag cggagaggaa cgagagccaa aacaagccct tgcacccgct
      481 caaaaatacc actgcgaaca aatcaacatt gatagctata ttcgctatga aaaaatcgat
      541 gcttacaacc tcgaaaacag cttagaagaa cgcttaaatc agttacttga tgacaacctt
      601 ctaagagggc ttttaatggt tggctggcaa ggcgtaagcc gtgcagatac atcaaacgcg
      661 acaaacaacc aactcgccca agacgtgaaa aaaggctggc tacaaaaaat cagagagggc
      721 aagcccgaag cggtaattaa tggagcaacc ataggcaaag agcaacaata caaaagccta
      781 aatgctctga ttaagcaggg attaggcaaa attagcccag cgtatgcaat gggcggagat
      841 atgatcgcca tttgtgggcg ggatattatc gctgatgatg taattcagct tgaacacaac
      901 gatttaagcg aaaacctagc cgacttatta accttatgcc aacaagctaa aggcggacta
      961 aaagcggtaa gtattcccta cttcccaagc aatgcgattt taatcactcg attagataac
     1021 ctttcgctat atcttcattt aaacaatatc cgccgtaaag cgtttgatac gccacaaaaa
     1081 gacagcgtag agacatttta ctccctcaat gtggattaca ttgtagaaga ttttaacgcc
     1141 tgttgtttaa ttgaaaatat tgaggttatt gaagaatgag cctagaaagc tatatccaac
     1201 aagcggaaga aatggagctt ttagaaaaac tcgattttgt ttcagacctg ttagaacttg
     1261 agaaagtcag cgaaattgaa gcctatcgca ttttaaccgc ccacggcgaa cctaaacccc
     1321 aaccggcaca agaaaagcta ataggttgcg tagattttct tgatgactaa cccaagcccc
     1381 gaaaggggct ttttgcattt ttgcaacaaa aaacgaccgc ttgcaccctt tgaaattatt
     1441 gctatcatta ataaaaaaca ataaccaaat caaatgagag tacagcaatg acaaactata
     1501 aactaccggc tagaaagtgg tacaccttag agcaggcaac aaaacgcatt aaacaactca
     1561 ctggcgaaga tatagaaata gaagatttat tacattattg gtatttaaga aagctagaat
     1621 taagtattta tttaa
//
LOCUS       DANLYI010000288         1604 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.290, whole genome shotgun sequence.
ACCESSION   DANLYI010000288 DANLYI010000000
VERSION     DANLYI010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1604
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.290"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            complement(13..189)
                     /locus_tag="PXD81_002452"
     CDS             complement(13..189)
                     /locus_tag="PXD81_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5538430.1"
                     /translation="MAYVRIYQGEAYGWCAEKGEANTECPEALLVSLTGDIFEAVGGD
                     KLKGAKKWQPYKKE"
     gene            203..460
                     /locus_tag="PXD81_002453"
     CDS             203..460
                     /locus_tag="PXD81_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5538431.1"
                     /translation="MICHADPEYVKYNYAGLWEKGSDGTFFYNYQQFMPEVEAKESLR
                     EQEPELDDGYNEEEYSEEEYSDENDASDENDNYDETYDDNF"
     gene            598..753
                     /locus_tag="PXD81_002454"
     CDS             598..753
                     /locus_tag="PXD81_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5538432.1"
                     /translation="MLFKEKGYDEFLTQKIAKGRAELESGKGIPLEQATLLVQSTIEE
                     TAQELDD"
     gene            807..965
                     /locus_tag="PXD81_002455"
     CDS             807..965
                     /locus_tag="PXD81_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5538433.1"
                     /translation="MTEEYQNFLIEQVKQGLDDIENGRVISLEESRQLCQKTIYETLQ
                     ELQGAVYA"
     gene            958..1275
                     /locus_tag="PXD81_002456"
     CDS             958..1275
                     /locus_tag="PXD81_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDL5538434.1"
                     /translation="MLSRKEVLMTRRASMNLQQIIRNVVEYTYSYESGDKLSAEIHNT
                     LDNLSYFPRIGVIGSVENTRECFCRGYRIIYRETENQVQILAIIHCRQQYPTDYYRSA
                     FKA"
     gene            1396..>1604
                     /locus_tag="PXD81_002457"
     CDS             1396..>1604
                     /locus_tag="PXD81_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5538435.1"
                     /translation="MSLLKKAQTFISIFNEVRAKNEAQRQSSNNLNVALKNAAEMRAE
                     MNGFSLKPQKRSTYFADHYGCGIHAV"
BASE COUNT          570 a          306 c          287 g          441 t
ORIGIN      
        1 cgtatccatc tgtcattctt tcttgtaagg ctgccatttt ttggcacctt tcagcttatc
       61 tccgccgact gcttcaaaaa tatcgccggt taaactcacc aataaggcct cagggcattc
      121 tgtatttgcc tctccttttt cagcacacca accataagcc tcaccttggt aaatacgaac
      181 atatgccacc cctcaattag ggatgatatg ccacgctgat cctgaatatg taaaatacaa
      241 ctacgccgga ctttgggaaa aaggcagcga tggcactttt ttctacaatt accagcaatt
      301 tatgcctgaa gttgaagcta aagaatcact aagggaacaa gaacctgaac ttgatgatgg
      361 atataacgaa gaggaatata gcgaagaaga atattccgat gaaaatgatg ccagcgatga
      421 aaacgataac tacgatgaaa cttatgatga taatttttaa atattcccaa accacacttc
      481 caaaaaccct gctcttgaca ctctattgct ttaagcgtaa attattccaa tatttcgcat
      541 ttaggagaac ataatcccta tgcctgaaag ccttgtaata agtgaaagga acataaaatg
      601 ctatttaaag aaaaaggcta cgatgagttc cttactcaaa aaattgcaaa aggcagagct
      661 gaattagaat ccggaaaagg tattcctctt gagcaagcca cattgttagt tcaatcaact
      721 attgaagaaa ctgctcaaga actggacgat taacaatttg taacattgct aatatatcta
      781 tatacaacta atgataggag ttatccatga cagaagaata ccaaaacttc ttaatagagc
      841 aagtaaaaca aggacttgat gatatagaaa acggacgtgt tatttcatta gaagaaagcc
      901 gtcaactgtg tcaaaaaacc atatatgaaa ccttgcaaga gctacaggga gccgtgtatg
      961 cttagtcgaa aagaggtgtt aatgactcgc agagcaagta tgaatttaca acaaatcatt
     1021 cgtaatgttg tggaatatac ttatagctat gaaagtggtg ataaattatc tgcggaaatt
     1081 cataacactt tggataatct ttcttatttt ccacgaattg gtgttatagg ttcagtagaa
     1141 aatactagag aatgcttttg ccgaggttat agaattatat atcgagaaac agaaaatcaa
     1201 gtgcaaattt tagccattat ccattgtcgt caacaatacc caaccgacta ttatcgctct
     1261 gcattcaaag cataacagga gctataaaat ggaaataaaa agaaaaatga ccgtagccga
     1321 attaaaactc aaggcaatgc tcgccaagcc taccatagtt caataatatt aggtgcaaaa
     1381 aacaggagaa ccacaatgtc tctacttaaa aaagcacaaa cgtttatctc tatttttaat
     1441 gaagtgcgag ctaaaaatga agctcaacgc cagtcttcaa acaatttaaa tgttgccttg
     1501 aaaaatgctg ctgaaatgag agctgagatg aatgggttct cccttaagcc tcaaaagcgt
     1561 tctacttact ttgctgacca ttatggttgt ggtattcatg cagt
//
LOCUS       DANLYI010000289         1602 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.291, whole genome shotgun sequence.
ACCESSION   DANLYI010000289 DANLYI010000000
VERSION     DANLYI010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1602
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.291"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            <1..321
                     /locus_tag="PXD81_002458"
     CDS             <1..321
                     /locus_tag="PXD81_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDL5538436.1"
                     /translation="KLSSELQNQINQLNQLRKQALKQEQDEQKLFEKNKIEDLPHFGT
                     QLNAQRKLLGIELTTLEMQTGISSSTLKRLFKDPSQVKFQTICIVAETLGFNLCAINT
                     HKQE"
     gene            complement(398..1416)
                     /gene="glpX"
                     /locus_tag="PXD81_002459"
                     /pseudo
     CDS             complement(398..1416)
                     /gene="glpX"
                     /locus_tag="PXD81_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484632.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
BASE COUNT          505 a          365 c          281 g          451 t
ORIGIN      
        1 aaactatcaa gcgaactaca aaatcagata aaccaactaa atcagttacg aaaacaggca
       61 ctcaaacaag agcaagacga gcagaaattg tttgaaaaaa ataaaattga agatttgcca
      121 cattttggca cgcagcttaa tgctcagagg aaactattag gcatagaatt aaccaccctt
      181 gaaatgcaaa ccggcatttc aagctctacc ctaaaacgct tatttaaaga cccaagccaa
      241 gtgaaatttc agacaatttg tattgtggca gaaacactcg gctttaattt atgtgcaatt
      301 aatactcaca aacaagagta agcgtgacaa tgtagtgatt ttgattactt tgggaagaca
      361 aaaaatcggg ctaaaagccc gatttatcag agaaaagcta taaatccaat aaagaaagga
      421 gttcgttatc tttacgatcg acataatggt cggattgaat tttacgaatg gttctagatt
      481 tgccacgaat aagtaaggtt tcagtgctgg caatattgcc tctacgttta atccctttta
      541 acaattcacc tgaagtaatg ccggttgcaa caaagacgat attgtcatca cgcaccaaat
      601 cttctaattt caacaccgta ttcaccggta cattcatttc attacagcgt aaaagctctt
      661 tttcggtagc agttaaattt tccgggctat tccccttcac ttggttgcga ggaattaaac
      721 gtgcttgcat atcgccaccc aatgcacgaa ccgcagcccc cgccactacc ccttcaggtg
      781 ctccgccgat accataaagc agatccaatt cgctctctgg caaacagcat tgcaccgcag
      841 ccgcaacatc gccatcagga atcgcaatca ctcttacccc taatttttgc acatcggcaa
      901 ttattttttc gtggcgaggc ttagcaagat agcaatggtt aattgggaaa gtaatttgcc
      961 ctttttcgag gcgacacggc gaagattttg ttcaagcgga agatttaaat caatcattcc
     1021 tttgcattcc tgccccacca ctaatttttc catatacata tcaggggctt ttaaaaaggt
     1081 ttctttgccg cctgctgcta ataccggcaa cgcatttgcc tgccccattg cagtcatacg
     1141 agtaccgtca atcgggtcaa ccgcaatact gatttcctca tcttctaggc gggatagacc
     1201 gattttctcg ccaatataaa gcatcggtgc ttcgtcaatt tcgccctcac caattaccac
     1261 ttcgccacgg atttccattt ggttcagcat aaaccgcatt gctttcaccg cagcttcatc
     1321 agcggcattt ttatccccac ggcctagcca agagtaagct gctagagccg ctgcttccgt
     1381 aacacgagaa aattcaaaag aaagagttcg tttcattttt gttccttagt ttcttgaaag
     1441 aaaaacttta aaagaagaga gattatattt tatcagtaac ctgcaagcta cgttactaaa
     1501 aaatcagcgt ttgaataaaa attcacaaat agccaaactt ttttataaaa ataaagtcag
     1561 atttagagta taatctttac aagtttttat attattttag ag
//
LOCUS       DANLYI010000290         1553 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.292, whole genome shotgun sequence.
ACCESSION   DANLYI010000290 DANLYI010000000
VERSION     DANLYI010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1553
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.292"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            <1..315
                     /locus_tag="PXD81_002460"
     CDS             <1..315
                     /locus_tag="PXD81_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HDL5538437.1"
                     /translation="FDRQDDWWHTGQNLYLDNIQGQGFERVGTPQIGDVILMQVGADV
                     PNHAAIYIGEQMVIHHSPNRLSKRDLYDGYWLRHTHSIWRHKLADKLDFDGILNDIAV
                     NN"
     gene            584..1174
                     /locus_tag="PXD81_002461"
     CDS             584..1174
                     /locus_tag="PXD81_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HDL5538438.1"
                     /translation="MITVKFYGHLRKFGTDFKLEVKDTAEAIRALCTQIRGLREALRD
                     GVYKVRIGKQYLDPSALEKGLFYCLKKGQTIHFTPVIKGAKSGGVFQAVLGVALIGAA
                     FFLGPVGWGVLGASSAMMMGAMGASMLLGGVSQMLTKMPKAPTMGNETEKESSTAFSN
                     LNNLVAQGKPVPLAYGLIRTGSLVISQGVETMTVEV"
     gene            1177..>1553
                     /locus_tag="PXD81_002462"
     CDS             1177..>1553
                     /locus_tag="PXD81_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5538439.1"
                     /translation="MGLFSRKRKNHTPKEAPETGRSKQIVNIVELLCEGEIEGLVDGF
                     KSIYLDGTKIQNDDDSYNFNNVSGQLNVGTQDQEVLEGYDSSQNEVSVGVEVKKKNGA
                     IVRTVTDERISRLRLTLGVRSLF"
BASE COUNT          474 a          287 c          371 g          421 t
ORIGIN      
        1 ttcgatcgac aagatgactg gtggcatacc gggcaaaatc tttatttgga taatatccaa
       61 gggcaaggtt ttgagcgagt gggaacccca caaatcggcg atgtgatttt aatgcaagta
      121 ggtgctgatg tgccgaacca cgctgccatt tacattggcg aacagatggt aatccatcat
      181 agcccgaacc gattatctaa gcgtgattta tacgacggct attggttacg tcatacccac
      241 agcatttggc ggcataaact ggcggataag ttagattttg acggcatatt gaatgatatt
      301 gctgtaaata actaacaaaa aatgaccgct tgtaacaaaa acaaaccccg aagcgtttgc
      361 agcacttcgg ggtttttcat atccacttac ctaactaaga ggacataaat taaatgaatg
      421 ataaacgatt tacattcaaa tttctaggag ttcttatgga agcaattaat attaccccaa
      481 aagaaatcag aaaaacaatg tggacagccg caataattct ctttttgttt gcgttcatct
      541 ggaaagcccc tgaatttatc actgctattc gttggtggta actatgatta cagttaaatt
      601 ttacggacac ttaagaaaat ttggtacaga ctttaagctt gaggtaaaag acaccgctga
      661 ggcaattcgt gccttatgta cacagattag aggattgcgt gaggcattgc gagacggtgt
      721 ttataaagtc cgtatcggta agcaatattt agacccatca gcccttgaaa aagggctttt
      781 ttattgcttg aagaaagggc aaaccattca ttttacccct gttatcaaag gggcgaaaag
      841 cgggggtgtt ttccaagctg tgttaggtgt agccttaatt ggagccgcat tttttctcgg
      901 gccggtcggt tggggagttc taggggcttc ttccgcaatg atgatgggag caatgggggc
      961 atcaatgtta ctaggaggag tttcccaaat gctcaccaaa atgccaaaag cccccacaat
     1021 gggtaatgaa acagaaaaag aaagctcaac ggctttttca aatttaaata acttggtggc
     1081 tcaaggcaaa cctgtgccgt tggcttatgg cttaattcgg accggctcgc ttgtgatttc
     1141 acagggagtt gagacgatga ctgtagaggt ataattatgg gattatttag tcgtaagaga
     1201 aaaaatcata ctcctaaaga agcaccggaa actggtcgct ctaaacagat tgtgaatatc
     1261 gtggagttac tctgcgaggg tgaaattgaa ggcttggtag atggctttaa atcgatttat
     1321 cttgacggca cgaaaatcca aaacgatgac gacagctata attttaataa tgtgagcggt
     1381 cagttaaatg tcggtacgca agatcaagag gtattagaag gttacgacag ctcacaaaat
     1441 gaagtgagtg tgggtgtaga agtcaagaaa aaaaacggtg caatcgtccg caccgttact
     1501 gatgaacgca tcagccgttt gcgtttaacg ttgggcgtgc ggtcactatt cca
//
LOCUS       DANLYI010000291         1551 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.293, whole genome shotgun sequence.
ACCESSION   DANLYI010000291 DANLYI010000000
VERSION     DANLYI010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1551
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.293"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            <1..1442
                     /locus_tag="PXD81_002463"
     CDS             <1..1442
                     /locus_tag="PXD81_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5538440.1"
                     /translation="KGSHIDADSVRAGILTAGAIRTEHLAAGQISADKLAIGLGGNLL
                     VNPIFATPDLSIAPFGWNYWRGEVGDRLNIDTRNIFNRNEQDNYGLKNGGLPNELVFS
                     MRYSTTKETSDTGRIGWLSQDINVVPNKKYIASVWLACHRGQAKLVIENIEKPGGAYL
                     GRIGNSEVITGYSAHQGEFKNMKRVKVVFTAPTSGCIRFALRLDNIQGKANPFLFARR
                     PMLEEASDNVTDITQPSPWQNAGVTAIHGGSIVTNTVTAQQIAANTITANEIATGTIA
                     ARNMAANSINASHIVGSSITADKLNVNNLAAISANLGKVTAGTITGTTITGNTISGGS
                     INGTTISGTTISGGTIKGARLEGATGKFTGELEVSQLIGGGVIEQVTGKLKYSHRNRI
                     YEGQEHGENSSSPIYRHEYIYKATVTINAAPTRRYIEIIGSESFILEANQSITKTIEK
                     SLNRNASGSSLDFIVLAYSISTAKYISIS"
BASE COUNT          494 a          283 c          348 g          426 t
ORIGIN      
        1 ttaaaggtag ccacattgat gcagactcag taagagccgg aatattgacg gcgggagcta
       61 tccgtacgga acacctggca gcagggcaaa tatcggcgga taagttagcg attgggctag
      121 gtgggaattt gctggttaat cctatttttg caacacccga tttgtcaata gctccgtttg
      181 gctggaacta ctggcgagga gaagttggtg atcggctaaa tattgacacc cgtaatattt
      241 tcaataggaa tgaacaagat aattacggac taaaaaatgg aggactacca aatgagttag
      301 tattttcgat gcggtatagc actacaaagg aaacctctga tactgggcga attgggtggt
      361 tatctcagga tattaatgtt gtgcctaata aaaaatatat tgcctccgta tggttagctt
      421 gtcatcgtgg gcaagctaaa ttagtcattg agaatatcga aaaacctggt ggtgcttact
      481 taggtcggat aggcaacagc gaggtaatta ctggctatag tgctcatcaa ggcgaattta
      541 aaaatatgaa acgggttaag gttgtattta ctgcacctac ttcaggctgt attcgtttcg
      601 cattacgatt agataatatt caaggtaaag ctaatccatt tttatttgct cgtaggccaa
      661 tgcttgagga ggctagtgat aatgttacag atatcactca gcctagccct tggcaaaatg
      721 ccggtgtaac cgctatccac ggtggatcaa tagttactaa taccgttact gctcaacaaa
      781 ttgcagcaaa taccattacc gccaacgaaa tcgcaaccgg cacgattgca gctcgaaata
      841 tggcagcaaa ctcgattaat gcaagtcata ttgtggggag ttcgatcact gcggataagc
      901 taaatgtaaa caatttagcg gcaatatcgg caaatcttgg taaggtaact gctggtacaa
      961 ttacaggtac gacaattact ggtaatacaa ttagtggcgg aagtattaat ggtacaacaa
     1021 ttagcggtac aactatctct ggcggcacaa taaagggtgc aagattggag ggggctactg
     1081 gcaaatttac cggcgaactt gaagtatctc aattaatagg gggaggtgtt atagagcagg
     1141 ttacaggcaa attaaaatat tcacatcgta atcgcattta tgaggggcaa gaacatggag
     1201 aaaactcctc ctctccaatt tatagacacg aatatatcta taaagcaact gtcaccataa
     1261 atgcagcgcc gacaaggagg tatattgaaa tcatcggaag tgagtcattc attttagaag
     1321 caaatcaatc aatcacaaaa acaatagaga aatcattaaa tagaaatgca agtggtagct
     1381 ctttagattt tattgtttta gcctattcca tttcgacggc taaatatatc tctatcagtt
     1441 agttatcgcc tcaatgtagt actgagtgcg gatagccaca gcttttttaa atatccaaaa
     1501 ccaaccgctt gcaggtcaat tcaggcgatt gtttttattt caactatcga t
//
LOCUS       DANLYI010000292         1541 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.294, whole genome shotgun sequence.
ACCESSION   DANLYI010000292 DANLYI010000000
VERSION     DANLYI010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1541
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.294"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            25..1374
                     /locus_tag="PXD81_002464"
     CDS             25..1374
                     /locus_tag="PXD81_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF560 domain-containing protein"
                     /protein_id="HDL5538441.1"
                     /translation="MAETVEFQQDPKEFDGQRQAVKFEQEIAKKDLKLTAYLEEDNSL
                     KGIEQHINQAILRQDWAKLERLLGDYAKQEQFDPLLLDYALAALAYAKKDYRSAAFYY
                     QQLLKKQPDLTYPRFDLALILKEDYRYREAEQEFKQLKTDDLRNAVNSALAQIEEEES
                     WKPDFYLQYTQTNNVNNASSSPTVNVNGRIFIKESDSLPQSAKGVRYGVGLSKMRNIG
                     GNHFVGVDLGYNGVYYWNNHDYSEQSVSLTPKYSYRTATYRIGVSPFIEQNWLGSSRY
                     NYQFGSSINAVKLFERGLISGSFSHLQKRYSEQHIADRYNGYRNQLTLGTQWQAVKNW
                     RFFANLNASREITREKAQSSNKWLATIGVIYQTPSWASSVSVGYGKRYFADKHYLFGY
                     KRQDKEYQANVAVWNRSWHWQGIVPKLNFRYQKIDSNIADFYSRSNKEVFMTLEKLF"
BASE COUNT          480 a          280 c          351 g          430 t
ORIGIN      
        1 tttttacttg tttcgccttt agtaatggcg gaaacggttg agtttcagca agatcccaaa
       61 gagtttgatg ggcagcgaca agcggttaaa tttgagcaag aaattgcaaa aaaggattta
      121 aaattaaccg cttacttgga ggaagataat tctcttaaag gtattgaaca gcacattaac
      181 caagcgattc ttagacaaga ttgggcgaag ctggaaaggc tgcttggcga ttatgcaaag
      241 caagagcaat ttgatccgct acttttagac tatgctttgg cggcattggc ttatgctaaa
      301 aaagattatc gctctgcggc gttttattat cagcagttgc tgaaaaaaca gcctgattta
      361 acctaccctc gttttgattt ggctctgatt ttaaaagagg attatcgcta cagggaagca
      421 gagcaagaat ttaagcaact caaaaccgat gatttacgca atgcggtgaa cagtgcgttg
      481 gctcaaatag aggaggaaga gagctggaag ccggattttt atttgcaata tactcaaacc
      541 aacaatgtga ataatgcttc ttcgtcgcca acggtaaatg taaacgggcg gatttttatt
      601 aaagaatcag acagcttgcc gcagtcggca aaaggcgtaa gatatggcgt tggcttgagt
      661 aaaatgcgaa atatcggcgg taatcatttt gtgggagtcg atcttggcta taacggcgtt
      721 tattactgga ataaccacga ttatagcgaa cagtcggtct cactaacacc aaaatatagc
      781 tacagaacgg caacttatcg tatcggggtt tcgcccttta ttgagcagaa ttggctgggt
      841 agctcacggt ataactatca atttggtagc agtatcaatg ctgttaaact ttttgaacga
      901 gggctgattt ccggctcatt cagccatctg caaaaacgtt atagtgaaca gcatattgcc
      961 gaccgttaca atggttatcg caaccaactc acgctaggta cgcaatggca agcggtcaaa
     1021 aattggcgat tttttgcaaa tcttaacgct agccgtgaaa ttacccgtga gaaagctcaa
     1081 tcttccaata agtggctggc gaccattggc gtaatttacc aaaccccaag ctgggcaagt
     1141 tcggtaagtg tcggatatgg caagcgttat tttgctgata aacattatct gtttggctat
     1201 aaacgccaag ataaggaata tcaagcgaat gtggcggtgt ggaatcgaag ttggcactgg
     1261 caagggattg tgccgaaatt gaatttccgt tatcaaaaaa tcgatagcaa tattgccgat
     1321 ttctactctc gtagcaataa agaagtcttt atgaccttag agaaactatt ttaaggctgt
     1381 gtatgaatgt cggggagaaa aattttctcc ccgatattta tgtgtagaag atcagacttg
     1441 atctgacaat tcactctaaa aagtgagagt ttcccgttta gaatatgagt gtcaaaaatc
     1501 aatctaaaca aaaaggaaac tctcatgttt tattctaaca a
//
LOCUS       DANLYI010000293         1538 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.295, whole genome shotgun sequence.
ACCESSION   DANLYI010000293 DANLYI010000000
VERSION     DANLYI010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1538
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.295"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            complement(<1..1407)
                     /locus_tag="PXD81_002465"
     CDS             complement(<1..1407)
                     /locus_tag="PXD81_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-histidine dipeptidase"
                     /protein_id="HDL5538442.1"
                     /translation="MSEISTLAPQLLWQWFDKACAIPHPSYHENELAEFIVNWAKSKN
                     FFVERDEVGNVLIRKPATKGMENRQTVALQAHLDMVPQANTGTVHDFTKDPIQPYIDG
                     EWVKAKGTTLGADNGIGLASCLAVLDSDDLAHPTIEVLLTMTEEAGMEGAIGLRPNWL
                     TADIMINTDTEENGEIYIGCAGGENVNVTLPIFLVPHQQDSAVTVTLKGLNGGHSGCD
                     IHTTRANAIKVFARILEQAHNEVEFQIAEIQGGSVRNAIPREAHATLVFSAKNQQKLT
                     ACLSQTAEQVKAELKIAEPNFQFLIEEAQLPEQAFDLASTARVIHFLNVMPNGVVRNS
                     DVVENVVETSLSIGVLNTEASEVKATILARSLIEGGKEEIRSKIRSLTALVGAEVEFS
                     GNYPGWEPNPNSKITPLTKAIYDEILGYESQIKVIHAGLECGLINKIYPNMDFVSIGP
                     TILNAHSPDEKVHIPAVEI"
BASE COUNT          392 a          354 c          333 g          459 t
ORIGIN      
        1 aatctccact gccggaatat gcactttttc gtccggagaa tgggcgttga gaatagttgg
       61 tccaatcgag acaaaatcca tattcgggta gattttgttg attaagccac attctagccc
      121 tgcgtgaatc actttaattt gagattcata acctaaaatt tcatcataaa tcgctttggt
      181 taatggcgtg attttggaat ttgggtttgg ctcccagccc gggtagttgc ccgaaaattc
      241 cacttctgct cccactaatg cggttaatga gcggatttta ctgcgaattt cctcttttcc
      301 gccctcaatt aatgagcggg caagaatggt cgctttcacc tcactcgctt cggtgttaag
      361 cacaccaata ctgagtgagg tttccaccac attttccacc acatcgctat tacgcaccac
      421 accatttggc atcacattta agaaatggat tactcgagca gtgctggcaa ggtcaaaggc
      481 ttgctcaggc agttgagctt cttcaattaa aaattggaag tttggctcgg caattttgag
      541 ttctgctttt acttgttcgg cggtttggct taaacaagcg gttaattttt gctgattttt
      601 tgcagagaaa accaaggttg cgtgggcttc tcgtggaatg gcattccgca ccgaaccgcc
      661 ttgaatttct gcaatttgga attccacttc gttgtgggct tgttccaaaa tgcgggcgaa
      721 cactttaatc gcattcgctc gggtggtgtg aatatcacag cccgaatgcc cgccgtttag
      781 gcctttaagc gtaacggtta ctgcactatc ttgctgatga ggcaccaaga aaattggcag
      841 tgtcacattc acattttccc cacctgcaca gccgatgtaa atttccccgt tttcttcggt
      901 gtcggtgtta atcataatat cggcggttag ccagttcggg cgtaaaccaa tcgccccttc
      961 cataccggct tcttcggtca ttgtgagcaa cacttcaatg gttgggtggg ctaaatcatc
     1021 gctatccaac accgccaagc acgaagccaa tccaatgccg ttatctgccc ccaatgttgt
     1081 gcctttggct tttacccatt cgccgtcaat ataaggctga atcggatctt tagtgaaatc
     1141 gtgaaccgtg ccggtattgg cttgtggcac catatcaaga tgggcttgca gagcgacagt
     1201 ttggcggttt tccatacctt tagttgccgg tttacggatt aacacattac ccacttcatc
     1261 acgttcaaca aagaaatttt tcgattttgc ccaattcaca ataaattcag ccagttcatt
     1321 ttcgtggtaa gacgggtgcg gaatagcaca ggctttatca aaccactgcc aaagtagctg
     1381 cggtgcgagc gttgagattt cagacataat aagccccttt tgtaaaattt taagaaaaaa
     1441 taaccgcttg tattcaagct gatatagacc tctgacattg tagaatattt aggagaaatt
     1501 taacaaaaaa atcttaaaaa gtacttgatg acgagttg
//
LOCUS       DANLYI010000294         1530 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.296, whole genome shotgun sequence.
ACCESSION   DANLYI010000294 DANLYI010000000
VERSION     DANLYI010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1530
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.296"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            complement(<1..>1530)
                     /locus_tag="PXD81_002466"
     CDS             complement(<1..>1530)
                     /locus_tag="PXD81_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5538443.1"
                     /translation="EQGKKLYSRVGYDKGVWEALSVLLPERFDFEVFEVIDYSKEWSW
                     FEIIVENLFWRQPEKINMQKLHQFFKNSPYFNQATWINILYQLATEINHPLNIKYLHN
                     KLYSFSLADRDEIWTAFIALESDQCPAIERLLTWCQKSSGQENIEPEALLLGAIAVSW
                     LFTSTDKRSRNGYINALAHLLWNRLEIALALFKEFIEVNDPYVMEGILSAIHGAVAYS
                     ENSSGLKALALEIDNQIFNQPETEEIYPNVLVRDYARHIIEYSLQKDEYSSDDIEIIR
                     ERITPPYRSSFPQNLPTNEEIDKKYHSDGQRIIIRSMTTEYGRGMCGYGDFGRYTFEA
                     KFRNWESYKFGNLINVDLLSNYACQMIFEQFGYDDKKHLEFDKETRSRYWRRDDTNKI
                     ERIGKKYQWLALYEILARVMDNFPTFSNRYADEIGLEYITNLDNFYLPKTQIKIVPPT
                     KTDVFFQYINQKQCKTKHYFTIQNDELIDDWVKINDFPDVSNLLEVTINDEQWLVLER
                     H"
BASE COUNT          498 a          281 c          215 g          536 t
ORIGIN      
        1 cgtgtctttc tagaacaagc cactgttcat cattaatcgt tacttccaaa agattactaa
       61 catcaggaaa atcatttatt tttacccaat catcaattaa ttcatcattt tgaatggtaa
      121 aatagtgttt tgttttacat tgtttttgat ttatgtattg aaaaaataca tctgttttag
      181 ttggcggtac aattttaatt tgggttttgg gtagataaaa gttgtccaaa ttagtaatat
      241 attcaagccc tatttcatcg gcatagcgat tagaaaaagt aggaaaatta tccatcaccc
      301 ttgccaagat ttcatacaaa gccaaccatt gatatttctt gccaattcgc tcaattttat
      361 tagtatcatc tcttcgccaa tatcttgaac gagtttcttt atcaaactca aggtgcttct
      421 tgtcatcata gccaaattgc tcaaaaatca tttggcaagc ataattactt aataaatcaa
      481 cattgatgag attgccgaat ttataactct cccaatttcg gaatttggct tcaaaagtat
      541 atctgccaaa atcaccatat ccacacatac cacgaccata ttcagttgtc attgaacgga
      601 taatgattct ttgtccatca gaatgatatt ttttatcaat ttcttcattg gttggtaagt
      661 tttggggaaa tgaacttcta taaggtggcg taattctttc tctgattatc tcaatatcat
      721 ctgatgaata ttcatctttt tgcaaagagt attcaataat atggcgagca taatcacgaa
      781 ccaaaacatt gggataaatt tcttctgttt caggttgatt aaatatttga ttatctatct
      841 ctaatgcaag tgcttttaag ccagatgaat tttcagaata agcaacagct ccgtgaatgg
      901 cagataaaat accttccata acataagggt cattaacctc tataaattcc ttgaataacg
      961 ccaatgcaat ttccaatcta ttccataata aatgagccag tgcattaata tagccatttc
     1021 tacttcgttt atcggtagaa gtaaatagcc aactaacagc aattgcaccc aataatagtg
     1081 cttcgggttc aatattttct tgaccacttg atttctgaca ccaagttagt aatcgttcaa
     1141 ttgctgggca ttgatcagac tccaaagcaa taaaagctgt ccaaatctca tctctatctg
     1201 ccaaagaaaa agaataaagt ttgttatgta aatattttat atttaatggg tggttaattt
     1261 cggtagccag ttgatataaa atattaatcc aagttgcttg attaaaatat ggactatttt
     1321 tgaaaaattg atgtagtttt tgcatattga ttttttcagg ctgcctccaa aagagatttt
     1381 ctacaataat ctcaaaccaa gaccattcct ttgaataatc aatcacttca aatacttcaa
     1441 aatcaaatcg ttctggcaac aatacactta atgcttccca tacgccctta tcatagccaa
     1501 ctctactata tagctttttt ccttgttcag
//
LOCUS       DANLYI010000295         1524 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.297, whole genome shotgun sequence.
ACCESSION   DANLYI010000295 DANLYI010000000
VERSION     DANLYI010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1524
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.297"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            535..810
                     /locus_tag="PXD81_002467"
     CDS             535..810
                     /locus_tag="PXD81_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014667974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDL5538444.1"
                     /translation="MAKATVARRIKNAINGNFGDHKSLAGTGGLYEMRIATGAGYRIY
                     YAQQGNVIYILLHGGDKSTQQADIEKAKALWAELKTTGESNGNNTNQ"
     gene            788..1111
                     /locus_tag="PXD81_002468"
     CDS             788..1111
                     /locus_tag="PXD81_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative addiction module antidote protein"
                     /protein_id="HDL5538445.1"
                     /translation="METIQINEQAAEKHGLRPFDVAEFLTDEATIQAYLAEVLKEGDQ
                     DEFLEALNNVARARGMAELAKQTGLGRESLYKTLAKGSKPRFDTIQKILGAFNLVLIP
                     QVKNG"
BASE COUNT          527 a          274 c          275 g          448 t
ORIGIN      
        1 ctaaaccttt atatatcaat gatttaatat tgaatttttg tcctattaac gctgaaattg
       61 tcctatttta gggtgttttt gtcctatttt caggggtttt tgtcctatca aaaaatgaac
      121 agtttactat tttgtcctag aattgtccta tattgtccta tattgtccta tgccaatagg
      181 acaattttag gggcgatgaa cccttgaaaa tcctagaatt gtcctattgt cctaattgtc
      241 ctatactttt ttctatattt tataaaagtg ttttggaaaa gtgatttttg ataaaaatcc
      301 aaatctaccc aaattgtaat tttatgctac ataatttaaa agcgtgaaca atgttcaatg
      361 atatgaataa ttgaaactgc tctcttttca aaatttaacc acttgattat ttgtatctta
      421 atggatacac taagcaataa atagaataag aggctggcaa tgacagagca aaccactaat
      481 caaatcatca ttaccgataa tttttcaaaa tagcttgaga atttaaagaa ccctatcgca
      541 aaagcaacgg tagcaaggcg gataaaaaat gcgattaatg gcaactttgg cgatcataaa
      601 tctttagccg gtacaggcgg actttatgaa atgcggatag caacaggagc cggatataga
      661 atttattacg cacaacaagg aaatgtaatt tatatcttgt tacacggcgg cgataaatca
      721 acacagcaag ccgatataga gaaagcgaaa gcattatggg ctgaattaaa aactacaggg
      781 gaaagcaatg gaaacaatac aaatcaatga acaggcagca gaaaagcacg gtttacgccc
      841 ttttgacgtg gcggagtttt taaccgatga ggcaactatt caagcctatt tagcggaagt
      901 gttaaaagag ggcgaccaag acgaattttt agaagccttg aacaatgtgg caagggcaag
      961 gggaatggcg gagcttgcca agcaaacagg gctagggaga gagagcctat ataaaaccct
     1021 agccaaaggc agcaaaccac gctttgacac tattcaaaaa attctagggg cattcaattt
     1081 agtgcttatt ccacaagtca aaaatggcta aaacttatca cttgctacct gccaaaacct
     1141 cacattcaaa tagccgttga ttgatattaa cggctttttt attgcttaat tctgtaccaa
     1201 ttacgcaccg attgcggatc aatacggggc aagttttcgc aaaaccttaa tgaaatatga
     1261 ccgcttgtga ttttgtacaa aacgacacaa accaatgtat aaaatcaaaa cttgccacaa
     1321 atggcggaat gatattaaat gccccacaaa ctagcagtat ttcttaatga cttttcattt
     1381 taagggtaca tttaagggta tgtttaacaa gtcttaaatg cacttctcaa taataacaag
     1441 cctcatagca tacaattcaa actcggctgg ggaccattta agcgttgcat attgtctttc
     1501 actgtctcaa atttactata aaat
//
LOCUS       DANLYI010000296         1486 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.298, whole genome shotgun sequence.
ACCESSION   DANLYI010000296 DANLYI010000000
VERSION     DANLYI010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1486
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.298"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            218..679
                     /locus_tag="PXD81_002469"
     CDS             218..679
                     /locus_tag="PXD81_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009874984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HDL5538446.1"
                     /translation="MTACPCQTGKRYAECCQPFHLKQAFASTAEQLMRSRYSAYTLIN
                     IPYIVETTVPSQQKLLDQTAMKEWAENTKWVGLSVLNHLPSLSKIHSQVEFKAFFDTD
                     EGIQAHHETSLFVQIDKRWFFVDPTVPLPHQKQPCVCGSGKKFKHCCGNYL"
     gene            751..1380
                     /locus_tag="PXD81_002470"
     CDS             751..1380
                     /locus_tag="PXD81_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HDL5538447.1"
                     /translation="MDFSKILSQVLDTAKDAATNGLIKGNSKNDQIAKIGGGAAAIGL
                     ISMLFGRNGGSSLAKLGSLAALGSLAYQAYQKYQAQNASASELSQNSFENVDKNVASK
                     VILQAMIAAAASDGAITEEEKAAIISQVGDDVEVIEWIRQEMDSPASVEEIARQVGND
                     QALATQVYLAARAVCTNLERKEIVFLANLAEALGLAEALVEQLEKDAGF"
BASE COUNT          483 a          243 c          286 g          474 t
ORIGIN      
        1 accaataaat taacctgtgg ataaaatctt aagttatcca caatctatag aataaaggct
       61 ttttaaagca atgaataccc aaaaaattca cgctttttgc ataagttatc cacaaggggt
      121 atgtcacgct tttttataaa atttattttt ctttatttat agcgtaaact tttcacatct
      181 ttatttttat gcggaaatgt aaaaatgtta gaaaaaaatc accgcttgtc cttgccaaac
      241 aggcaaacgc tacgctgaat gttgccaacc tttccattta aaacaggcat ttgcatcaac
      301 ggcagagcaa ttaatgcgtt cccgttacag tgcttatact ttgattaata ttccctatat
      361 tgtggaaacg acagtgccaa gtcagcagaa attacttgat cagactgcaa tgaaagagtg
      421 ggctgaaaat acaaaatggg taggattatc cgtgctaaat cacttacctt ctctttcgaa
      481 aatacatagt caggttgaat ttaaagcctt ttttgataca gatgagggga tacaggcaca
      541 ccacgaaacc tctctttttg ttcaaattga taagcgatgg ttttttgttg atcccaccgt
      601 acctttgcct catcaaaaac aaccttgtgt atgtggttcc ggaaagaagt ttaagcactg
      661 ttgtggaaat tatctttaat ttgaattgaa ctcttgtttg aatctaagga ctaatgagta
      721 aatttcattt acttaccaag aggaacaaaa atggatttca gtaagatttt aagtcaagta
      781 ttagatacag caaaagatgc tgctacaaac ggattaatta aaggtaattc taaaaacgat
      841 caaatcgcta aaattggtgg cggcgctgcc gctattggtt taatttcaat gttatttggg
      901 cgtaatggcg gttcaagttt agcgaaatta ggctctttag ctgctttagg tagccttgct
      961 tatcaggcat atcaaaaata ccaagcacaa aatgcatcag catcagagtt aagccaaaat
     1021 agttttgaga atgttgataa aaatgtagca agtaaagtga ttttacaagc aatgattgcc
     1081 gctgccgctt cagatggtgc tattactgaa gaggaaaaag cagctatcat ttctcaagtt
     1141 ggagatgatg tagaagtaat tgagtggatt cgtcaagaaa tggattcccc agcttcagtc
     1201 gaagagattg ctcgtcaagt aggtaatgat caagcattag ctactcaagt ttacttagct
     1261 gcccgggcag tatgtaccaa tttagaacgc aaagaaatag ttttcttagc taatctagct
     1321 gaagctctag ggttagcaga ggctttagtg gaacaattag aaaaagatgc aggcttttaa
     1381 ttttgcttat ttttagtaac aagcggttat attttgagag ttttttgcga aaaacttgtg
     1441 aaatatgacc gcttgttctt ttttatgttt tattgtaaaa attgta
//
LOCUS       DANLYI010000297         1479 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.299, whole genome shotgun sequence.
ACCESSION   DANLYI010000297 DANLYI010000000
VERSION     DANLYI010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1479
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.299"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            18..836
                     /locus_tag="PXD81_002471"
     CDS             18..836
                     /locus_tag="PXD81_002471"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphatase"
                     /protein_id="HDL5538448.1"
                     /translation="MNYQAIAFDLDGTLLSSNATILESSKEAIKKVREKGIKVYFVTG
                     RHHTAVRPYYVELGLDTPVVCCNGTYVYDFKNEKVLSGNPLSAEIASKLVNDAQAQGI
                     HTAVYFEDAMTYESLNIHFTKFQKWVESCAPEVRPNVYQVENFQQEIDKGVTVWKVLI
                     SDPDLAKMQKFVEKLPPDQVSPEWSWVDRVDITSVGNSKGARLAEVLKTEGIDLQKVI
                     AFGDNFNDVSMLETVGLGIAMGNSEPDIQQKAKKTIGSNNEDSIANELKAQFNL"
     gene            893..>1479
                     /locus_tag="PXD81_002472"
     CDS             893..>1479
                     /locus_tag="PXD81_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pili methyl-accepting chemotaxis
                     transducer N-terminal domain-containing protein"
                     /protein_id="HDL5538449.1"
                     /translation="MIGFASIISGISLGIMWSNKSDASLINVSGSLRMQSYRLLSEMD
                     SKKALLPERLLEYHRTLHATELSSLKQSVLLPDSVIESYQQLLKDWYEMQSYVAQGDK
                     VNYASHIEDYVQRVDDFVFRLQEFAELKLKIATGVIAIAMLLIIALAYIGVWYTRKKI
                     IGPLRQLVKASWQIQNKDFDHVKLAVNEPNELGLLS"
BASE COUNT          492 a          219 c          316 g          452 t
ORIGIN      
        1 atttataggg gaatactatg aactaccaag ctattgcatt tgatttagac ggaacactac
       61 tttcatcgaa cgcaacaatt ttagaatcca gtaaagaggc tattaaaaag gtaagagaaa
      121 agggaattaa agtctacttt gttaccggta ggcaccatac tgcggttcgc ccttattatg
      181 tagaactagg gctggatacg ccagttgtgt gttgcaatgg cacttatgtg tatgatttta
      241 aaaatgaaaa agtattaagc ggtaatccgt tatcagcaga aattgcttca aaattagtta
      301 atgatgcaca agctcaaggg attcatactg cggtttattt tgaagatgca atgacttatg
      361 agtctttgaa tattcacttc actaaattcc aaaaatgggt tgagtcttgc gcaccagaag
      421 ttcgtccgaa tgtttatcag gtagaaaatt tccaacaaga aattgataaa ggtgtaaccg
      481 tatggaaagt gcttattagc gatccagatc ttgccaaaat gcaaaaattt gttgaaaaat
      541 taccgcctga tcaagtaagc ccggaatggt cttgggttga tagggttgat attaccagtg
      601 ttggtaacag caaaggggca agattagcgg aagtattaaa aacagaaggt attgatttac
      661 aaaaggtgat tgcctttggc gataatttca atgatgtttc tatgctagaa accgttggat
      721 taggcattgc aatgggaaat tcagaaccag atattcagca aaaagcgaag aaaactatag
      781 gttcaaataa tgaagatagt attgctaatg aattgaaagc tcaatttaat ctttaattat
      841 gataaaagca aaatactcta ttgctaggcg gataggactc tattttttaa ttatgatcgg
      901 atttgcttcg attatcagtg gaatctcact tggtattatg tggagcaata aatctgatgc
      961 gagcctgatt aatgtttcag gctcacttcg tatgcaaagt tatcgattgt tatcagaaat
     1021 ggattcaaaa aaagcgctgc tacctgagcg attgttggaa tatcatcgaa cgctacatgc
     1081 tacagagctt agttcattaa agcaaagtgt tttattgcca gatagcgtga tagaaagcta
     1141 tcaacaatta cttaaagatt ggtatgaaat gcaatcttat gtcgctcagg gagataaagt
     1201 taattatgct tcgcacattg aagattatgt gcagagagtt gatgattttg ttttccgctt
     1261 acaagagttc gctgagttaa aattaaagat tgcaacaggc gtgattgcga ttgcaatgtt
     1321 actgattatt gcccttgctt atattggcgt ttggtatact cgaaaaaaga ttattggacc
     1381 tttaaggcaa ttggtaaaag cgagttggca aattcaaaat aaagattttg accacgtaaa
     1441 attagcggtt aatgaaccca atgaattagg gctactttc
//
LOCUS       DANLYI010000298         1478 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.300, whole genome shotgun sequence.
ACCESSION   DANLYI010000298 DANLYI010000000
VERSION     DANLYI010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1478
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.300"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            65..1381
                     /gene="argA"
                     /locus_tag="PXD81_002473"
     CDS             65..1381
                     /gene="argA"
                     /locus_tag="PXD81_002473"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HDL5538450.1"
                     /translation="MQNTELALAQWFRQSTPYVNMHRGKTFVIMLDGDVIESPNFVNI
                     INDISLLHSLNIKLVIVFGARPQINQLLKQNHIEPEYYHNIRVTNPQSLEYVKQAVGK
                     VHYDFFARLSLRLPHSEVINVVSGNMILAQPIGVVDGVDYTLSGKIRRINIESIKQQL
                     AQNSIVLLGPIAPSVTGEMFNLPFEDIATQVAIKLRADKLIGFCEEQGILDENGKPIP
                     DYLPQDGETLLQQLIERGEYHTPKARFLQAAITVCREGVKRSHLLSYKEDGSLLQELF
                     SRDGIGTQFSMESSEQVRIANSSDIPGLLDLIRPLEQQGILVKRSREQLEMEITNYTI
                     IERDGVVIACAALNPYPEEKMAEMACVAVHPDYRDSSRGDILLENIQKRAVQLGMEKL
                     FVLTTRTTQWFQERGFRLAGAQDLPRYKRENYNYQRRSKVLILPLW"
BASE COUNT          458 a          275 c          327 g          418 t
ORIGIN      
        1 aatttaaggc ttgcggattt ttctaaaaaa gataatatgc gtttatatta taaagaggaa
       61 atatatgcaa aacacagaac tcgcattagc tcaatggttt aggcaatcta caccttatgt
      121 taatatgcac agaggcaaaa cctttgtgat tatgcttgat ggcgatgtta tcgaaagccc
      181 gaattttgtc aatattatca atgacatcag tctcctgcac agcctgaata tcaaattagt
      241 gattgtattt ggggcaagac cgcaaattaa ccaattgtta aaacaaaatc atattgagcc
      301 ggagtattac cacaatatcc gtgtgactaa tccacaatcg ctagaatatg tgaaacaagc
      361 ggtagggaaa gttcattatg atttctttgc ccgcctttct ttgcgtttac cccattctga
      421 agtgattaat gtagtcagtg gtaatatgat tttagctcag ccgattggcg tggtggatgg
      481 ggtagattat acactaagcg gtaaaatccg tcgtattaat attgagagca ttaagcagca
      541 actggcacaa aattcgattg tattacttgg tccgattgcc ccgtctgtta ccggtgaaat
      601 gtttaatctg ccgtttgaag atattgccac acaggttgca attaaacttc gtgccgataa
      661 attaatcggt ttttgtgaag agcaggggat tttagatgaa aacggcaagc caattccaga
      721 ttatcttccg caagatggcg aaaccttgtt acagcagtta atcgagcgtg gcgaatatca
      781 caccccgaaa gctcggtttt tgcaggcggc gatcacggta tgccgagagg gagtgaaacg
      841 ctcgcattta ttaagctata aagaagatgg ctcattgctt caagagttat tctctcgtga
      901 tggtattggc acacagttct caatggaaag ctcggaacag gtacgtattg ctaattccag
      961 tgatattccg ggattattgg atttaatccg cccacttgaa cagcagggca ttttagtcaa
     1021 acgctctcga gaacaattag aaatggaaat cacaaactac accattattg agcgtgatgg
     1081 tgtggtgatt gcttgtgcgg cacttaaccc gtatccggaa gaaaaaatgg cggaaatggc
     1141 gtgtgtggcg gttcaccctg attatcgtga ttcctcaaga ggggatattc tactggaaaa
     1201 tattcaaaaa cgggcagtgc aactgggaat ggagaaactc tttgtgctga ctacaagaac
     1261 cacacaatgg ttccaagagc gaggatttag gctggcaggt gcccaagatt tacctcgcta
     1321 taaacgggaa aattacaatt atcaacgtcg ttctaaagta ttaattttac cactttggta
     1381 aaatattcaa tgtttttgac cgcttgttac caagagaact ataaaaggag aactatgaaa
     1441 aaacatcaac ccatcaaagg ggctatggca attattac
//
LOCUS       DANLYI010000299         1476 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.301, whole genome shotgun sequence.
ACCESSION   DANLYI010000299 DANLYI010000000
VERSION     DANLYI010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1476
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.301"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            <1..183
                     /locus_tag="PXD81_002474"
     CDS             <1..183
                     /locus_tag="PXD81_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HDL5538451.1"
                     /translation="PNHAAIYIGEQMVIHHSPNRLSKRDLYDGYWLRHTHSIWRHKLA
                     DKLDFDGILNDIAVNN"
     gene            452..1081
                     /locus_tag="PXD81_002475"
     CDS             452..1081
                     /locus_tag="PXD81_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HDL5538452.1"
                     /translation="MITVKFYGHLKTFGTDFKILVKDIAEAIRALCSQLKGLREALRD
                     GVYKVRIGKQYLDPSALEKGLFYCLKKGQTIHFTPVVKGAKSGGIFQAVLGVALIGAA
                     FALGPIGLGLVGSATAMTVGAMGASMLLGGVSQMLTKMPKAPTMGNETEKESSTAFSN
                     LNNLVAQGKPVPLAYGLIRTGSLVISQGVETITIKENQPASNKKTGFRK"
     gene            1091..>1476
                     /locus_tag="PXD81_002476"
     CDS             1091..>1476
                     /locus_tag="PXD81_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL5538453.1"
                     /translation="MRIYGAKGGGGSSHTPIEAPETGRSKQIVNIVELLCEGEIEGLV
                     DGFKSIYLDGTKIQNDDGTYNFNNVSGQLNVGTQDQDVLDGYDSSQNEVNVGVEIKKK
                     NGAIVRTVTDERINRLRLTLGVRSLF"
BASE COUNT          457 a          277 c          347 g          395 t
ORIGIN      
        1 ccgaaccacg ctgccattta cattggcgaa cagatggtaa tccatcatag cccgaaccga
       61 ttatctaagc gtgatttata cgacggctat tggttacgtc atacccacag catttggcgg
      121 cataaactgg cggataagtt agattttgac ggcatattga atgatattgc tgtaaataac
      181 taacaaaaaa tgaccgcttg taacaaaaac aaaccccgaa gcgtttgcag cacttcgggg
      241 tttttcatat ccacttacct aactaagagg acataaattg aatgaatgat aaacgattta
      301 cattcaaatt tctaggagtt cttatggaag caataaatat taccccaaaa gaaatcagaa
      361 aaacaatgtg gacagccgca ataattctct ttttgtttgc gttcatctgg aaagcccctg
      421 aatttatcac tgctattcgt tggtggtaac tatgattaca gttaaatttt acggacacct
      481 taaaacattc ggcacagatt ttaaaatttt agttaaagac atcgctgagg caattcgtgc
      541 tctgtgttca cagcttaaag gattgcgtga agcgttgcga gacggtgttt ataaagtccg
      601 tatcggtaag cagtatttag acccatcagc ccttgaaaaa gggctttttt attgcctgaa
      661 gaaagggcaa accattcatt ttacccctgt tgtcaaagga gcgaaaagcg gtggtatttt
      721 tcaagcggtg ttaggtgtgg cgttaattgg ggctgcattt gcgttaggtc ctatcggctt
      781 aggattggtt ggctctgcta cagcaatgac agttggagca atgggggcat caatgttact
      841 aggaggagtt tcccaaatgc tcaccaaaat gccaaaagcc cccacaatgg gtaatgaaac
      901 agaaaaagaa agctcaacgg ctttttcaaa tttaaataac ttggtggctc aaggcaaacc
      961 tgtgccgttg gcttatggtt tgattcgtac cggctcgctg gtgatatcgc agggagttga
     1021 gacgatcacc attaaagaaa accaaccggc aagcaacaag aaaaccggct ttagaaaata
     1081 agggataatt atgcgtattt acggagcaaa aggtggtggc ggcagtagcc ataccccgat
     1141 tgaagcaccg gaaaccggtc gctcaaaaca gattgtgaat attgtggaat tgctgtgcga
     1201 gggcgaaatt gaaggcttgg tagatggctt taaatcaatt tatcttgacg gcacgaaaat
     1261 ccaaaacgat gacggcacct ataattttaa taatgtgagc ggtcagctaa atgtcggtac
     1321 gcaagatcaa gatgtgttgg acggttacga tagctcacag aatgaagtga atgtgggtgt
     1381 tgagattaag aaaaagaatg gggctattgt ccgcaccgta actgatgaac gaattaaccg
     1441 cttgcgttta acgttgggag taaggtcatt attcca
//
LOCUS       DANLYI010000300         1474 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.302, whole genome shotgun sequence.
ACCESSION   DANLYI010000300 DANLYI010000000
VERSION     DANLYI010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1474
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.302"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            24..>1474
                     /locus_tag="PXD81_002477"
     CDS             24..>1474
                     /locus_tag="PXD81_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HDL5538454.1"
                     /translation="MLSKLQRFTLMIKSTIIKHPLEILFVTYVTILLINNVSDYELIK
                     RFQNLIALFPICFILLYLIRDTKAYWLFAPIPIAIVFFFGQINLTLLEDSRYWATIFC
                     VFLCLISQYWYKNNHYFVAGTTNKLVNIVFATVLSGIIFATLSAITIAIIALFDLNKQ
                     NNFHIFEKFGIFSSFWAFPVLFLTLEQQTTNDSRYLNRISEMLLNWILSPALIIYTAI
                     IYAYVIFIAAKGQMPDGVVANVALPYLAFGLAIQAVQLLLKNAKWQWFYSHFTYLALL
                     PLTLIWYAIYIRFSHYGLTEARIYLVIGTITLSICYTLLLFKQLAQYRLFSAVSIAII
                     LISVFILDPKTVALKDQTKRLDNYLLKHKLLDVNNKISTKAVLKYQESLNENRDEAEY
                     FYSMLHYVARGMPAAQFKEKYGVNSSYDLMSEIRERNTDPRYFEATNSELVRLTKADM
                     ANAREYITFSSQLYRPNSFSHQKIENKLCQKLLS"
BASE COUNT          462 a          265 c          224 g          523 t
ORIGIN      
        1 ttttcatagg caggagatct attatgctaa gtaagctaca acgctttacc ttaatgataa
       61 aaagcaccat cattaagcat ccactggaaa ttttattcgt tacttacgtc acgattttac
      121 ttatcaataa tgtgagcgac tacgaattaa tcaaacgctt tcaaaatcta attgctcttt
      181 ttcctatttg ctttattttg ctttacctga tacgcgatac caaagcttat tggctattcg
      241 ctccgattcc gatagcaatc gtctttttct ttggtcaaat taatttaaca ttattggaag
      301 attctcgtta ttgggctacc atattttgtg tattcctttg tttgatcagt caatattggt
      361 acaaaaacaa ccattatttt gtagccggaa caactaataa attagttaac attgtctttg
      421 ctacagtttt atctggcatt atttttgcaa cattaagcgc tattaccata gccattattg
      481 ctttatttga tttaaataaa caaaacaact ttcatatttt tgaaaaattt ggcatttttt
      541 ctagtttttg ggcattccct gttttatttt taactcttga acaacagact accaatgatt
      601 ctagatatct aaaccgcatt agtgaaatgt tactaaactg gattttgtct ccggcattaa
      661 ttatctatac tgcaattatt tatgcttatg taatttttat tgcggctaag gggcaaatgc
      721 cagatggggt tgttgcaaac gtagcgcttc cctatttagc tttcggccta gctatacaag
      781 cggtgcaatt attactaaaa aatgcaaaat ggcagtggtt ttattctcac tttacctatt
      841 tagcactttt gccgttaaca ttaatttggt atgccatcta tattcgtttc agccattatg
      901 gcttaactga agcccgtatt tatttagtga ttggcacaat aaccttatct atttgctata
      961 ccttattact atttaaacag ttggcgcaat atcgtctctt ttctgcagtg tcgatagcaa
     1021 ttattttaat ttcagtgttt attttagatc cgaaaacagt tgcgcttaaa gatcaaacca
     1081 agcggttgga caactatttg ctaaaacaca aattattaga tgtaaataac aaaatcagca
     1141 caaaagctgt acttaaatac caagaatcat taaatgaaaa ccgagatgaa gccgaatatt
     1201 tttattcaat gctccactat gttgccagag gaatgcctgc tgcacaattt aaagagaaat
     1261 atggcgtaaa ttcaagctac gatttaatga gtgaaataag agaaagaaat actgacccac
     1321 gttattttga agctactaat tctgagctgg ttcgcttaac taaagcagat atggcaaatg
     1381 ctcgtgaata tattacattt agtagccaat tatatcggcc taattcattt tcccatcaaa
     1441 aaatagaaaa taagctatgc caaaaactcc tttc
//
LOCUS       DANLYI010000301         1364 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.303, whole genome shotgun sequence.
ACCESSION   DANLYI010000301 DANLYI010000000
VERSION     DANLYI010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1364
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.303"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            complement(80..>1364)
                     /locus_tag="PXD81_002478"
     CDS             complement(80..>1364)
                     /locus_tag="PXD81_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL5538455.1"
                     /translation="IRQKIAHNKMTSFQWLVVGLTVLLNMLDGFDLMALAFTAKSIRE
                     ELQLDGAAIGSLMSAGLFGMAIGSLFLSPLADKFGRRPLLLIGTGLATLGMLLTYFAT
                     SAFEIGVWRVLTGLGVAAILTGTNVLVSEYSSQKWRGLAIAIYASGFGIGAMLGGMSA
                     VLLQGEYGWRAVFLVGSILTGAVFVLLFFVLPESVEFLISKHPVNAKARLNQIAVKLG
                     LGNGWELPEIEAKSKSKVAITRLFEPEYRRNTLLIWLAFIAISASFYFISSWTPALLE
                     EAGMPKTQSQTVGMAISVGGTIGSLIFGFLVSRWAAKHILQAFSILAASAVVAFVFAG
                     NLGLAITLAILVGGLINGCITGLYTINPTLYAPDFRSTGVGTAIGVGRLGSIVSPTLA
                     GILLDAGWKKNELYLGAAVVLLIAAFTVHFLKQRK"
BASE COUNT          434 a          320 c          277 g          333 t
ORIGIN      
        1 aataaattta atcaaaaaaa ttatataatc ttgccccgta attaattgaa taaaacctca
       61 attaattacg gggtagctct tattttctct gttttaaaaa atgtaccgtg aaagcagcaa
      121 ttaataagac gactgctgca cctaaataaa gctcgttctt tttccagccg gcgtctaata
      181 aaatacctgc aagagttggg gaaacaattg agcctaaacg ccctacaccg atggcggtgc
      241 ctacgcccgt gctgcggaaa tccggtgcgt atagggttgg gttaatggta taaagcccgg
      301 taatgcagcc gttaattaat ccgccaacca aaatggctag tgttatcgca agccctaaat
      361 tgccggcaaa cacaaaggca accacagcag atgcagccag aatactgaat gcctgcaaaa
      421 tatgttttgc cgcccagcga ctgactaaaa agccgaaaat taacgagccg attgtaccac
      481 caactgaaat tgccattccc acagtttggc tttgggtttt aggcattccg gcttcttcta
      541 atagtgccgg agtccaagag ctgataaagt aaaatgaggc tgaaattgca ataaaggcaa
      601 gccaaattag caacgtattg cggcgatatt caggctcgaa taaacgggta attgccactt
      661 tgcttttact ttttgcctca atttcaggaa gttcccagcc gtttcctaag cctaatttaa
      721 cggcaatttg atttaaacga gctttagcat tcaccgggtg ttttgaaatt aaaaattcca
      781 ccgattcggg caatacaaag aataataaaa cgaataccgc accggttaaa atcgacccaa
      841 cgaggaaaac cgcacgccag ccatattctc cttgcaataa caccgcagac attccgccca
      901 gcattgcacc aatcccaaag cctgaggcat aaattgcaat cgctaagcca cgccattttt
      961 ggctggagta ttcactcact agcacattag tgccggttaa aatggcggct acgcctaaac
     1021 cggttaaaac acgccataca ccaatttcaa acgcagaagt agcgaaataa gttaggagca
     1081 ttcctaacgt ggctaagcct gtgccgatta aaagcaacgg gcggcgacca aatttatcag
     1141 caagtggtga aagaaagagt gagcctatcg ccataccgaa taaaccggca ctcattaagc
     1201 tgccgatggc tgcaccgtct aactgcaatt cttctcggat gcttttggcg gtaaaggcga
     1261 gtgccattaa atcaaagccg tccagcatat taagtaacac cgtaagccca accactaacc
     1321 attggaatga agtcattttg ttatgagcaa ttttttgacg aata
//
LOCUS       DANLYI010000302         1335 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.304, whole genome shotgun sequence.
ACCESSION   DANLYI010000302 DANLYI010000000
VERSION     DANLYI010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1335
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.304"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            <1..582
                     /gene="purU"
                     /locus_tag="PXD81_002479"
     CDS             <1..582
                     /gene="purU"
                     /locus_tag="PXD81_002479"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005641775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HDL5538456.1"
                     /translation="VILVTKEAHCLGDLLMKNYYGGLDVEIAAVIGNHETLKSLVERF
                     DIPFHLVSHENLTRVEHDKLLAEKIDEYSPDYIVLAKYMRVLNPEFVARYPNRVVNIH
                     HSFLPAFIGAKPYHRAYERGVKIIGATAHFVNDELDEGPIIMQNVINVDHTYTAEAMM
                     RAGRDVEKTVLSQALELVLADKVFVYKNKTIIL"
     gene            complement(619..1302)
                     /locus_tag="PXD81_002480"
     CDS             complement(619..1302)
                     /locus_tag="PXD81_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HDL5538457.1"
                     /translation="MNQTRDRLEKLPLFHKMQQFIIKALKIIAIFPLLGIAILLVVDS
                     LTSYIVRDKIYTDSKQIPYREYAVVLGTAKFYSKDVINQYYKYRLEAAKQLVQEGKVN
                     QLLLSGDNKTPYYNEPKTMTSDLLKMGIATSKIKQDYAGYTTFYSIIRAKKVYKLPPF
                     TIISQKFHCERALFIAKFRDIDAICYAATYPEGAYQVRMREILARSAMVFSLLIGKEP
                     ESLEDIRSK"
BASE COUNT          416 a          258 c          229 g          432 t
ORIGIN      
        1 gttattttag ttaccaaaga agcacattgt ttaggtgact tactgatgaa aaattactat
       61 ggtggattag atgtagaaat tgctgccgtg attggaaacc acgaaacatt aaaaagttta
      121 gttgaacgtt tcgatattcc attccattta gtcagccacg aaaatttaac ccgagtggaa
      181 cacgataaat tattagcgga aaaaattgat gaatattcac ctgattatat tgtgttggct
      241 aaatatatgc gggtgctgaa tccggaattt gtcgctcgtt atccaaaccg agttgtcaat
      301 attcaccact cgttcttacc cgctttcatt ggtgcaaaac cttatcatcg tgcatacgaa
      361 cgtggagtga aaattatcgg tgcaaccgcc cattttgtga atgatgaatt agatgaaggc
      421 ccgattatta tgcaaaatgt gattaatgtt gatcacacct acaccgcaga ggctatgatg
      481 cgtgccggac gtgatgtcga gaaaaccgta cttagccaag ccttagaatt agtgcttgct
      541 gacaaagtat ttgtatataa aaacaaaacc attattttat aagcaaaata gccggtcaac
      601 attatctgac cggctatctc atttagaacg gatatcttcc aatgattcag gctctttacc
      661 tatcaacaaa ctaaaaacca ttgccgatct ggctaaaatt tcccgcattc gtacctgata
      721 agccccctct ggatatgtag cggcataaca aattgcatca atatccctaa attttgcaat
      781 aaacaatgcc ctttcacagt ggaatttttg ggagataatc gtaaatggcg gtaatttata
      841 tactttttta gccctaatta ttgaataaaa agtggtatac cccgcataat cctgcttaat
      901 ttttgaagtc gcaatcccca ttttaagtag atcacttgtc attgtttttg gctcattata
      961 gtacggcgtt ttattatctc cacttaacaa aagctgattg acctttcctt cttggactaa
     1021 ttgcttagcg gcttccaaac gatatttata atattggtta atcacatctt tagagtaaaa
     1081 ttttgccgta ccgagtacaa ctgcatactc ccggtaagga atctgtttac tatctgtata
     1141 aattttatcc ctcacaatat aacttgtaag tgaatcaacc actaataata tagctattcc
     1201 caataagggg aaaattgcaa ttattttcaa tgccttaata ataaactgct gcattttatg
     1261 gaaaagtggc aatttttcta acctatcacg cgtttggttc aaatgagatc ctttactttt
     1321 gctaccctat ttaag
//
LOCUS       DANLYI010000303         1324 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.305, whole genome shotgun sequence.
ACCESSION   DANLYI010000303 DANLYI010000000
VERSION     DANLYI010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1324
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.305"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            131..406
                     /locus_tag="PXD81_002481"
     CDS             131..406
                     /locus_tag="PXD81_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5538458.1"
                     /translation="MKNNILITYDLNKSGQNYAALIEKIKTLGAWAKVQQSAWYLHTS
                     YSSKEVLEMLSTVTDYNDSLFVAQLSEANWRGLSNEVSKFIQEQWWK"
     gene            complement(409..660)
                     /locus_tag="PXD81_002482"
     CDS             complement(409..660)
                     /locus_tag="PXD81_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5538459.1"
                     /translation="MKNKNFEIAKVVILNEQLGSPKDLELKVVENGLSDEDKYHIKQA
                     VLKAVENGCLDPEMLASRCCSAIETINKSGINTGNGSCE"
     gene            complement(1113..>1324)
                     /locus_tag="PXD81_002483"
     CDS             complement(1113..>1324)
                     /locus_tag="PXD81_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5538460.1"
                     /translation="NNHKDYILCGRKEKRTSDFINVFEVFENETTQEFVIERAMFRNG
                     KLIDWNQSDKMNAEQAQQLWQAYIH"
BASE COUNT          365 a          280 c          229 g          450 t
ORIGIN      
        1 aacacacttg gtgaaatatg ctttggaata agtgcctttc tttatacttg taaggctcaa
       61 gtcccttatt gcctctcaca actctaagga atataatcaa ggctctcaca actcaaataa
      121 ggattaagtt atgaaaaata atattttaat tacttatgat ttaaataaat cagggcaaaa
      181 ttacgcagca ctcattgaaa aaattaaaac tcttggagct tgggctaaag tacagcagtc
      241 agcttggtat cttcatactt catattcctc aaaagaggtt ttagaaatgc taagcaccgt
      301 aactgattac aatgactctc tttttgttgc tcaattatcc gaggctaatt ggagaggatt
      361 atccaatgag gttagtaaat ttattcaaga acaatggtgg aaatagacct attcgcaact
      421 tccattacct gtatttattc cgcttttatt gattgtttca attgcagaac aacatcgtga
      481 tgcaagcatt tctgggtcca agcaaccatt ttctacagct ttaagtacag cctgtttgat
      541 atggtattta tcctcatcag acaggccatt ctctacaacc tttaattcca aatctttagg
      601 gcttcctagt tgttcattta aaataacaac tttcgcaatt tcaaaatttt tgtttttcat
      661 aattacctcg tgtttaaatt attccaaaac ccactcataa taggctttgg aataaatgcg
      721 tgtctgccca cgccgtcgcc cgccctgcat ttcgcctgtt cagtcgggtg ctgcttgtat
      781 ccgtacttac tcaaacttga gataagcgtg attgcttagg actgatacca gtgttgcctt
      841 ttcagctctc cacaccttgc gagtgtgttg cctaaacctt gccacctctg tttggcaagt
      901 cgttcaaatt tgtatgtcag ggtctaacca accttgccga gtgataaagc attactttac
      961 aactgctcga ggttgttaat atcgtccgca caagcactaa attgttaaag aacatcccac
     1021 agtctctgct tatctctcgg ctattgcctg ctatgtgggc taggtctaga acctttattc
     1081 aagccctcag taaagggctt tgataaaaat tcttagtgaa tgtaggcttg ccaaagttgt
     1141 tgagcttgtt ctgcgttcat tttgtcgctt tggttccagt cgattaactt gccatttctg
     1201 aacattgctc tttcaatcac aaactcttgc gtggtttcat tttcaaagac ctcaaacaca
     1261 ttgatgaaat cactagtgcg tttttcttta cgtccgcata aaatgtagtc tttgtggtta
     1321 ttta
//
LOCUS       DANLYI010000304         1315 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.306, whole genome shotgun sequence.
ACCESSION   DANLYI010000304 DANLYI010000000
VERSION     DANLYI010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1315
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.306"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            <1..329
                     /locus_tag="PXD81_002484"
     CDS             <1..329
                     /locus_tag="PXD81_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glucose-1-phosphatase/inositol phosphatase"
                     /protein_id="HDL5538461.1"
                     /translation="VGHDSNIIALLSALNVKPYKLAHQYEQTPIGGKVFIEIWKEKSS
                     QAQKVKLEYVYQSTDQIRKAIPLSLDKPPQHHILSLDYCQADQYGLCDYMEFKQLLEK
                     VIADTL"
     gene            637..>1315
                     /locus_tag="PXD81_002485"
     CDS             637..>1315
                     /locus_tag="PXD81_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL5538462.1"
                     /translation="MSQDIQADLKKILALLSRNRRFQSLFLLLLRSEQFINSDALSTE
                     LQLSVRTVKKEIKLLKEKLEPYGIQIFSRPFYGYKLLIQFEELHTQLKQYFQISQPMT
                     VNNKFESRVNAILRRLLVSQRALKVEDFQSELYLSFTSALTKEFSQVKALLGKYGLIL
                     EAKPYHGMNIVGESYRKIMLTVRLYKYFTQLEHPFGIKEFEQCFACPMTEKIRERLAK
                     TIIHFNLV"
BASE COUNT          461 a          217 c          229 g          408 t
ORIGIN      
        1 tagtcggaca cgattccaat attatagcct tactttctgc attaaatgtt aagccttata
       61 aattagcaca tcaatatgaa caaactccaa taggagggaa agtctttatt gaaatctgga
      121 aagaaaaaag tagccaagca caaaaagtta aactggaata tgtatatcaa tctactgacc
      181 aaattcgtaa agcgattcca ctaagccttg ataaaccacc gcaacatcac attttgagtt
      241 tggattattg ccaagctgac caatatggat tatgcgatta tatggaattt aaacagttac
      301 tggaaaaagt cattgctgat acattgtgag attttgacaa agtgagtgaa aaaggtagca
      361 taaaaaagtc gggacagttg ccagctgctc cctgtaagat gttgtttaca gcactagtaa
      421 tgctactatc cccaaaaagc tgctgaaagg cagctttttt gttgtatata aaagtacacc
      481 tttctacgct tcattctttt tctccaatta aacctaattt aggacttcta gtgataaatt
      541 cggtatcata tcttaattca ctacatatcg tgcaagcctt aaaattgaaa gggctgacga
      601 aatcctattt aattctgaca cacataaggg aatattatgt cgcaagatat tcaagccgat
      661 ttaaaaaaga tattggcact attaagccgt aaccgtcgat ttcaatcatt atttttatta
      721 ctgttaagaa gtgaacaatt tattaatagt gatgcacttt ctacggagtt acagctttca
      781 gtacgaacgg taaaaaaaga gattaagtta ttaaaagaaa aacttgaacc ttacggcatt
      841 cagatttttt ctcgaccgtt ttatggctat aaattattga ttcagtttga agaattacac
      901 acgcaactaa aacagtattt ccaaatatca cagccgatga cggtaaataa taaatttgag
      961 tctcgggtaa atgcgatttt acgccgatta ctggtgagcc agcgtgcatt gaaagttgaa
     1021 gattttcaga gtgagcttta tttaagtttt accagtgcct taacaaaaga atttagtcaa
     1081 gttaaagcct tgttaggaaa atatggattg atactcgaag cgaaacccta tcacggaatg
     1141 aacattgtag gtgaaagtta tcgtaaaata atgctcacag tacgattata taaatatttt
     1201 acccagcttg aacatccatt tggaattaaa gaatttgaac agtgttttgc ttgtcctatg
     1261 actgaaaaaa tccgtgaaag gttagctaag acaattattc atttcaattt agtat
//
LOCUS       DANLYI010000305         1274 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.307, whole genome shotgun sequence.
ACCESSION   DANLYI010000305 DANLYI010000000
VERSION     DANLYI010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1274
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.307"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            complement(794..>1274)
                     /locus_tag="PXD81_002486"
     CDS             complement(794..>1274)
                     /locus_tag="PXD81_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transferrin-binding protein"
                     /protein_id="HDL5538463.1"
                     /translation="PSLFIFHKNYEKPVNIKGIELEFSYDIGSFYGNLAYARQNTNQP
                     VSFTDASSRVDAASHYGFKTQGFGASKISALPRDYGSLELGTRWFDQRLQIGGVAKYY
                     GKSKRIAIDEFNYTYYPGTHIVKETHRLEETVNKQPIIVDFYVSYEPIKSLILKLEL"
BASE COUNT          434 a          241 c          194 g          405 t
ORIGIN      
        1 ataaatggat atgagttggc ataatacaat aggcatacaa aatcattccc ttatgttttc
       61 tgcagtaatc taaggcatca acaattacct gaaaataaat ctctctcaca aacacaccaa
      121 cccaataaac agttgcaaaa ctgacaaaat aagcaccgct cttttcgtgg aatttatatt
      181 ttcggctcat agcctttctc aacttaaaaa ggaataagaa aaatacccta ttcaaaatta
      241 aattccacaa gccaatataa ctttttcgat ctagatcgca aaaatcatta aaacttgcaa
      301 agtgttgata aaatttaacc gcttgtgata agccaaagaa atactgaaat gatagcaatg
      361 gatggataaa taaaatgatg acgcaagcgt ccacgcttgt gccaaaaaca aaagcccaaa
      421 taaaccaaat tttacttggc tgatttaggc ttttatctta tgactgaaat actttcagaa
      481 caggaattaa ctggcacaag cgtggacgct tgcaccatct gggttaaatc cttatattat
      541 aaggttttaa cctactgtgc agactcaatt ttagtcacta tgccaaataa ttagttaaag
      601 gaaatttaag ctagcttatc taggacaacc ccatttagaa tttataactt gtgctaaaca
      661 ctatagttct ccctcttgca taattattga gcgtataaat aggttcatca ctgatatcag
      721 aattgtaaac cctctgactt gctgcatcat tattagcatc aagaggatca atgtactttt
      781 tgtcaaagac attttatagt tctaacttta atataagcga cttgattggt tcgtagctca
      841 cgtaaaaatc aacaataata ggttgtttat ttacggtttc ttcaagccga tgagtttctt
      901 tcacaatatg tgttccagga taataagtat agttaaactc atctatcgca atacgtttac
      961 tttttccgta atattttgcc actccgccaa tttgtagtct ttgatcaaac catcttgtcc
     1021 ctaactccaa tgagccatag tctcttggta acgctgaaat tttgctggca ccaaaacctt
     1081 gagttttaaa accataatga gaagcggcat ctacccttga acttgcatct gtaaaggata
     1141 cgggttgatt cgtattttga cgagcataag ctaagttgcc atagaaagag cctatatcat
     1201 aactaaattc taattctatc cctttgatat ttactggttt ttcatagttt ttgtggaaaa
     1261 tgaataaact agga
//
LOCUS       DANLYI010000306         1262 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.308, whole genome shotgun sequence.
ACCESSION   DANLYI010000306 DANLYI010000000
VERSION     DANLYI010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1262
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.308"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            32..751
                     /locus_tag="PXD81_002487"
                     /pseudo
     CDS             32..751
                     /locus_tag="PXD81_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005616566.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
     gene            811..1260
                     /locus_tag="PXD81_002488"
     CDS             811..1260
                     /locus_tag="PXD81_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudoazurin"
                     /protein_id="HDL5538464.1"
                     /translation="MLAKLKWNQLACVTLAFCSASVFSAHHEIKMLDNNAEGSMVFEP
                     GYVKAEVGDTVTFIPSHKGHWVESRSIPEGAEKFLSKEDEEFTITLTHEGVYVYYCPP
                     HRTMNMAGIIQVGQALNLEATQKEVEKIEKRTKENKGRLFEYLEQVK"
BASE COUNT          412 a          224 c          285 g          341 t
ORIGIN      
        1 gttttagtcg ttatctagga cagcccctac ttttaatact cgtaagcccc cttttgataa
       61 ccctaaagtt cgtcaagcat tgaattatgc ggtgaataaa actgaaattt taacttccgt
      121 ttaccaaggc tctgcaacgc cagctaaaaa cccaatgcct tctacgattt ggggatataa
      181 caacgaagta gccggttatg aatttaatcc ggaaaaggcg aagagcttgc ttaaagaggc
      241 cggattagaa aatggctttg aaacagaaat ttgggcaatg cctgtttctc gcccttacaa
      301 tccaaatgcc cgtagaatgg ctgaaatgat tcaagaggat tggaaagcaa taggggtaga
      361 ggcaaagata gtgagctatg aatggggaga atatcttaaa cgaatgggaa acggcgagca
      421 cgctaccgga atgattggtt ggaccggaga taacggcgat ccagataatt tcctcaatgc
      481 cctactcagt tgtaatgcgg tagaacaagg ctcaaactat gcagggtttt gtaatccaga
      541 gtttgaccgt ttaattaatg aggctgcgac tatcagtgat caaaacaagc ggtcagattt
      601 atataaaaaa gcgcaattca tctttaatga gcaagcacct tggttaccga ttgcacattc
      661 aacggtgtat tttccggtaa ggaaagaggt aaaaggctat gttgtcagcc cgtttattgc
      721 tcataatttt tatggtgttg agctggaata acctaaggta taatgaattg aactcatttt
      781 gtgcctcata aatttaggag caataaatat atgttagcga aacttaagtg gaatcaatta
      841 gcttgtgtaa cattggcatt ttgttcagcc tcagtatttt cagcccatca cgaaatcaaa
      901 atgttagata acaatgcgga aggatcaatg gtatttgagc ccggttatgt aaaagcagag
      961 gtgggcgata cggtaacatt tattccgtct cataaagggc attgggttga aagccgctcg
     1021 attccggaag gtgcggagaa gttcttatct aaagaagatg aggaatttac tattaccctt
     1081 actcacgaag gtgtttatgt ttactactgt ccgccacacc gaaccatgaa tatggccgga
     1141 attatccaag taggacaagc tctcaatctt gaagccacac aaaaagaagt ggaaaaaatt
     1201 gaaaaacgca cgaaagaaaa taaaggacgt ttatttgaat atttagagca agttaagtag
     1261 aa
//
LOCUS       DANLYI010000307         1240 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.309, whole genome shotgun sequence.
ACCESSION   DANLYI010000307 DANLYI010000000
VERSION     DANLYI010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1240
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.309"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            complement(<1..547)
                     /locus_tag="PXD81_002489"
     CDS             complement(<1..547)
                     /locus_tag="PXD81_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein Hlp"
                     /protein_id="HDL5538465.1"
                     /translation="MNTLAKISLSVLFTLFLTACEQPNSTKTQSSAESPVQVKEESKE
                     EVKPADTGAQDYKMLREWQDTQEKALNDAIKAATETLTDKQKADSTLMQETVNNALLA
                     QIDHIKISAETLNIQNNEVKALKDKTLEVLTLGAQMIVEGAKMEKNPTPEAHKAFGEL
                     QTKLNQLAEEGQQLENILRAKY"
     gene            complement(614..1198)
                     /locus_tag="PXD81_002490"
     CDS             complement(614..1198)
                     /locus_tag="PXD81_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein HlpB"
                     /protein_id="HDL5538466.1"
                     /translation="MNKLTKISATALVALFLAACDKPAEKAPATEPTKTTEAAPTTTA
                     PAAEAAKPAETAVAAPSEAAKADYQKLVDWNTAQESTMATAQAELQKKLATQDPKQIQ
                     EGLSAFNKKVEETVKSLDAIEVSDAQIKTFKEKTKSVLTLSSEVISEQVKTISTPNDQ
                     AALQAVQQKAQSLIEAGNELQKLNVELQQRFSAK"
BASE COUNT          294 a          210 c          232 g          504 t
ORIGIN      
        1 catatttagc tcttaatata ttttctaact gttgcccttc ttcagctaat tgattcaatt
       61 ttgtttgtaa ctcgccaaat gctttatgtg cttccggtgt aggatttttc tccattttag
      121 caccttctac gatcatctga gcccctaaag tcaggacttc taaggttttg tcttttagtg
      181 ctttgacttc attattttga atattcaggg tttcagcact tatcttaata tgatcaattt
      241 gagctaataa tgcgttatta actgtctctt gcattaaggt agaatcggct ttttgtttgt
      301 cagtaagtgt ttctgttgcc gctttaattg catcatttaa ggctttttct tgagtgtctt
      361 gccattcccg taacatttta taatcttgag cgccggtatc agcaggttta acttcttctt
      421 ttgactcttc tttcacttga actggcgatt ctgctgagct ctgtgttttg gtagaattag
      481 gttgttcgca agcggttaaa aaaagggtaa ataatacaga taggctaatt ttagcgagcg
      541 tgttcatagt aatccttagt aattgcaaaa aaaagctaga gcaaaatact ctagcttttc
      601 attgaatggt aaattattta gctgagaatc tttgctgtaa ttctacgttt aatttttgta
      661 actcattacc agcttcaatt aaagactgag ctttttgttg tacggcttgt aatgctgctt
      721 ggtcatttgg tgtagagatc gtttttactt gttcagaaat cacttcactt gaaagtgtta
      781 aaactgattt tgttttttct ttaaatgttt tgatttgtgc atcagatact tcaatcgcat
      841 ctaagctttt tacagtttct tctacttttt tattaaatgc acttaatcct tcttgaattt
      901 gtttaggatc ttgtgtagct aatttctttt gtagttctgc ttgtgctgtt gccattgtgc
      961 tttcttgtgc cgtattccaa tcaactaatt tttggtaatc tgcttttgca gcttcgcttg
     1021 gagcggctac agctgtttca gcaggtttgg ctgcttctgc agcaggagct gttgtagttg
     1081 gtgcagcttc agttgttttt gtaggttctg ttgccggtgc tttttctgct ggtttatcac
     1141 aagctgctaa gaataatgcg actaaagcgg ttgcactgat ttttgttaat ttgttcatta
     1201 aaaaattcct caagaatata aaaataaaag ccaataaagg
//
LOCUS       DANLYI010000308         1204 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.310, whole genome shotgun sequence.
ACCESSION   DANLYI010000308 DANLYI010000000
VERSION     DANLYI010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1204)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1204)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1204
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.310"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            <1..990
                     /locus_tag="PXD81_002491"
     CDS             <1..990
                     /locus_tag="PXD81_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5538467.1"
                     /translation="SSSTPNHPKPVLVPKTQNNLQAQNVPQAQNASQAQNAPQAQNAP
                     QAQNAPQVENAPQAQNAPQVENAPQAEVTPPVPQPQSQKIDGSFDKIGSVKLNKEAQT
                     LELSRFTLVDKLGTPPKFDKVSGKKIIEEKDFLVLNLSDINAEQLSGDFLIRRSDDLF
                     YGYYHDTNGKNLVDAADKFSQYFVVYDEKRVNDNISDKLTATYRKKEGFVYGSNPHTK
                     EFAARISKLGDVEIKFENGQAQGSIKDEKDGNAEIFTIKGDTKQLEITPTESNRIIIA
                     ILDQNQKSYTPGMEKAIMETKFIDSKAGNSDQKYLIGEAKSDNWQAIMVSEKK"
BASE COUNT          440 a          233 c          215 g          316 t
ORIGIN      
        1 tcgtcttcaa caccgaatca ccccaaacca gtactagtac caaaaacaca aaataatctt
       61 caagcacaaa atgttcctca ggcacaaaat gcctctcagg cacaaaatgc ccctcaggca
      121 caaaatgctc ctcaggcaca aaatgctcct caggtggaaa atgctcctca ggcacaaaat
      181 gctcctcagg tagaaaatgc tcctcaagca gaggttactc cgcctgtacc acagccacaa
      241 tcacaaaaaa ttgacggttc ttttgataaa attggttcag taaaactcaa taaagaggct
      301 caaactcttg agcttagtag attcactttg gtggataaat taggcacacc accgaagttt
      361 gataaagtaa gcggtaaaaa aattatagaa gaaaaagatt ttctcgtatt aaatttgtct
      421 gatattaatg ctgaacaact ctctggcgat tttcttattc gccgtagcga tgatctattc
      481 tatggctact atcacgatac aaatggcaaa aatcttgtcg atgctgccga taaattcagt
      541 caatattttg tcgtgtatga tgagaaacgg gtaaatgata atatctctga taaattaaca
      601 gcaacttacc gtaaaaaaga aggctttgta tatggttcaa atccacatac taaagaattt
      661 gccgcacgga tcagcaaatt gggggatgta gaaattaaat ttgaaaatgg tcaagctcaa
      721 ggaagtataa aagacgaaaa agatggaaat gctgagatct ttactattaa aggtgataca
      781 aaacagttag agattacccc aacggaaagt aaccgaatca ttatagcaat tttagaccaa
      841 aatcaaaaaa gctatactcc aggaatggaa aaagcaatta tggaaactaa gtttattgat
      901 tcaaaggctg gtaattccga ccaaaaatac ttaatcggtg aagcaaaaag cgataactgg
      961 caagcaataa tggttagcga gaaaaaataa agttatcttt tgctaaaaac tgaaataaaa
     1021 aggctgagtc cgggtaatat cggcctcagt cttttaaatt gtagaaaatc atctgtagaa
     1081 gatcaaacct aatctgacag tcccccgttt taaattaccg tgtctgtcag attaatttga
     1141 gcttaaattc ttttccaccc aaatccgttt tccatcaagt aaggttgcca tcggtgttct
     1201 gcca
//
LOCUS       DANLYI010000309         1178 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.311, whole genome shotgun sequence.
ACCESSION   DANLYI010000309 DANLYI010000000
VERSION     DANLYI010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1178
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.311"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            174..743
                     /locus_tag="PXD81_002492"
     CDS             174..743
                     /locus_tag="PXD81_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinG"
                     /protein_id="HDL5538468.1"
                     /translation="MANKPPKQHKCKECGSYYIKFQSTQQVCSVKCAMAMGKRKTEAK
                     RKKSAKIDRLEQRKRLEKLKSRRDFEKDLQKIFNKFIRLRDKDLPCISCGRYHQGKYD
                     AGHYKTVGGNPELRFNEDNCHSQCVPCNRHLHGNIVNYRVNLIEKIGLERVEFLERKD
                     HPPLKLTIEQIKDLIKVYKAKCKELERVT"
     gene            733..>1178
                     /locus_tag="PXD81_002493"
     CDS             733..>1178
                     /locus_tag="PXD81_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5538469.1"
                     /translation="MLPRKIKNKWLEPEKQKWIEELLRLWGAWEFGGLDLESRVNMIY
                     RLMKSAEGVKSLSSREVCNDSLGTLINEIFTVVTKRDVMLADILKQKYWFGRSERQIA
                     IYYQLRDSEGRKERRWQEIIQDKCKKAERIIADILESKISSSNSTD"
BASE COUNT          394 a          180 c          289 g          315 t
ORIGIN      
        1 ttgttattgg ttgtggttta tttctgttat gcggaatgtt atttaaggag attaataagt
       61 gagccagtgc aatgaactga ctgtaatttt aatcgccatc gctattctta gtgtatgtgc
      121 cggttcactc ttttttattg agtggtttga taagagagat ggtgagggtt ggaatggcta
      181 acaaaccacc taaacaacac aaatgcaaag agtgcggcag ttattacatc aaatttcaaa
      241 gcacgcagca ggtctgctct gttaagtgtg ctatggcaat gggtaagcgt aagacagagg
      301 cgaaacgcaa aaaaagtgca aaaatcgacc gcttggagca acggaagcgg ctggaaaaat
      361 tgaaaagccg tagagatttt gagaaagatt tacaaaagat atttaataaa ttcattcgct
      421 tgcgtgataa agatttaccc tgtatttcct gtggccgcta tcatcagggg aaatatgatg
      481 cagggcatta taaaaccgtt ggtggtaatc ctgagctacg ttttaatgaa gataattgcc
      541 acagtcaatg tgtgccgtgt aaccgacatc tgcacggcaa tattgtgaat tatcgagtaa
      601 atctgattga gaaaatcggg cttgagcgtg tggaattttt agagcgtaaa gaccacccac
      661 cattaaaact tactatcgaa caaatcaaag atttaatcaa agtctataaa gcgaagtgca
      721 aggagcttga gcgtgttacc tagaaaaatt aaaaacaagt ggttagagcc tgaaaagcag
      781 aaatggatcg aagagttgct aaggttatgg ggggcgtggg aatttggcgg attggattta
      841 gaaagtcggg taaatatgat ttaccggctg atgaaatctg cggagggggt taaaagtctt
      901 tcatctagag aggtttgtaa cgatagcctc ggcacgctga tcaatgagat ttttacggta
      961 gtcaccaaaa gagacgtaat gttagccgat atactcaaac aaaaatactg gttcgggcgt
     1021 tcggagcggc aaatcgctat ttattatcaa ctaagggaca gcgagggtag aaaagaaaga
     1081 cgatggcaag aaattattca agataaatgc aaaaaagcgg aaagaattat tgcagatatt
     1141 cttgaaagca agatttcatc cagtaattct acagataa
//
LOCUS       DANLYI010000310         1060 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.312, whole genome shotgun sequence.
ACCESSION   DANLYI010000310 DANLYI010000000
VERSION     DANLYI010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1060)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1060
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.312"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            complement(87..296)
                     /locus_tag="PXD81_002494"
     CDS             complement(87..296)
                     /locus_tag="PXD81_002494"
                     /inference="COORDINATES: protein motif:HMM:NF015431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDL5538470.1"
                     /translation="MPLNIWQKAVALLADSTVDRLVAENGLVEVLGETENPKFDLTMI
                     YAAHRYPSSIVRAFVGFVLEQVKAN"
     gene            complement(238..948)
                     /locus_tag="PXD81_002495"
     CDS             complement(238..948)
                     /locus_tag="PXD81_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDL5538471.1"
                     /translation="MNLNTLRLFVAVIQHGSLSKASERLNVPIATISRQIADLEKELN
                     IQLFDRQKSGVKPTMAGQRLYEQVHLSIEQLATAKEVLFEEQGNLKGKLRLSASPACE
                     PVLAWIADFQAAYPQVQVHCTLTDRLLDLSADGIDVAFRIGQLHGEQFIAKKVGQIGS
                     KWVAHPKFLARFGTPSTVKDLKNFPIAAWAKNEASVITLGTGRQAVEIPYLFSSNDSY
                     AIEYMAKSSGSACRQHGG"
BASE COUNT          286 a          245 c          228 g          301 t
ORIGIN      
        1 attaaaagag tagttgcaaa aaaacataaa aaaatgaccg cttgtgagtt taaaatccac
       61 aagcggtcat ttttctttaa aaatactcaa ttagctttta cttgttccaa cacaaaccct
      121 acaaacgccc gcacaatgct agacggataa cgatgggcgg cataaatcat cgtcaaatca
      181 aatttggggt tttctgtctc acccaagact tccaccaagc cattttctgc caccaatcta
      241 tccaccgtgc tgtcggcaag cagagccact gctttttgcc atatattcaa tggcatagct
      301 gtcattgctg gaaaaaagat agggaatttc taccgcttgt ctgccagtgc ctagcgtgat
      361 gacgcttgct tcattttttg cccaagcggc aatggggaaa tttttcaaat ctttgacggt
      421 gctgggcgtg ccaaagcgtg ccaaaaattt agggtgtgcc acccatttac tgccgatttg
      481 ccccactttt ttggcgataa actgttcgcc gtgtagttgc ccaatccgaa acgccacatc
      541 aatgccatct gccgacaaat ccaaaagacg gtcggtcagc gtgcaatgca cttgtacttg
      601 tggataagct gcctgaaaat cagctatcca cgccaaaaca ggctcgcagg cagggcttgc
      661 cgacaggcgt aatttaccct ttaaattgcc ttgctcttca aacaacactt ctttggcagt
      721 ggcaagctgt tcaatggaca aatgcacctg ttcatacagg cgttgccctg ccattgtggg
      781 tttaacgcct gatttttggc ggtcaaagag ttgaatgtta agctcttttt ccaaatcggc
      841 aatttgacgg ctaatggttg caatcggcac attgagtcgc tcggaggctt tggagagact
      901 gccgtgctgg ataacggcga caaagaggcg gagggtgttg aggttcattg tttttctccc
      961 caatgttcaa tttcttgaat aaaatgttta atactgcctt ggacttgttg atttttacca
     1021 tatagataat aaacgcctga aagttcttgt aaaaatttac
//
LOCUS       DANLYI010000311         1054 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.313, whole genome shotgun sequence.
ACCESSION   DANLYI010000311 DANLYI010000000
VERSION     DANLYI010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1054)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1054)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1054
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.313"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            2..>1054
                     /locus_tag="PXD81_002496"
     CDS             2..>1054
                     /locus_tag="PXD81_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDL5538472.1"
                     /translation="MAIYAIWNNKGGVGKSYLTFQIACEYAKQYPAKKVLVVDLCPQA
                     NSSSMLLGGIEKGEQELAKIHSQVNKNTISGYISERLVSPYRSINSATKYVTQVSTIN
                     EYVPKNVYLVVGDEELEIQSSGILHASQAVHVPDSWRLVHLWVRDLVSDIQNAWDNDD
                     NVVFIDCNPSFTIYTELALAASDRLIIPFSADGSSKRAVKSVLSLVYGVKRHSGDVSS
                     HFYTESQKSKLALPQIYMYVGNRLTINAKASATAFKTVVEEIGDEIYNIWKTNANLFC
                     IHPAGVGTPTNKSSFRKMFQYEVRDANTASVVSGALGIPISRLSSGKRDVLGKNVMVN
                     QSQLDAQVPNIQNLVQC"
BASE COUNT          343 a          159 c          204 g          348 t
ORIGIN      
        1 tatggctatt tatgcaatat ggaataataa aggtggggtt ggtaaaagtt atttaacttt
       61 tcaaatcgct tgtgagtatg caaaacaata cccagcaaaa aaagttctcg ttgttgattt
      121 atgtcctcaa gcaaattcat catctatgct attaggcggc attgaaaaag gtgagcaaga
      181 gttagcaaaa attcattctc aagtaaacaa gaatactatt tcaggatata tttcagaaag
      241 attagtaagt ccttaccgtt ctattaatag tgcaacgaaa tatgtaactc aagttagcac
      301 tataaatgaa tatgtaccaa aaaatgttta tcttgttgtt ggtgatgagg aattagaaat
      361 ccaatcatct ggtattttac atgcttccca agctgttcat gttcctgatt cttggcggct
      421 agtgcatctt tgggtgcgtg atttagtttc tgatatacag aacgcttggg ataatgatga
      481 taacgttgta tttattgatt gcaatccaag tttcactatt tatactgaat tagcgttggc
      541 tgcttcagat agattgatca tccctttttc cgctgatggt tcatcaaaac gagctgttaa
      601 gtctgtatta tctctagtat atggggttaa aaggcactct ggggatgtca gctctcattt
      661 ttatacagaa tctcaaaaga gtaagttagc attacctcaa atttatatgt atgtaggtaa
      721 tagattaacg ataaatgcca aagcatcagc aaccgcattt aaaacagttg tagaggaaat
      781 aggtgatgaa atctataata tatggaaaac taatgctaat ttgttctgta ttcacccagc
      841 aggtgttggt actcctacaa ataaatcaag cttcagaaaa atgtttcagt atgaggtgag
      901 agatgcaaat acagcatctg ttgtttcagg tgctttaggt attccaatta gtcgcttatc
      961 atcaggaaaa agagatgttt taggtaaaaa tgtaatggtg aatcagtcac aattagatgc
     1021 tcaagttcct aacattcaaa atcttgttca gtgt
//
LOCUS       DANLYI010000312         1051 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.314, whole genome shotgun sequence.
ACCESSION   DANLYI010000312 DANLYI010000000
VERSION     DANLYI010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1051
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.314"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            <1..1011
                     /locus_tag="PXD81_002497"
     CDS             <1..1011
                     /locus_tag="PXD81_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005600133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HDL5538473.1"
                     /translation="WLYNLGSYVPKSPSSEQIFQWTNAVREGDSGQFVTDLVPRNHLY
                     RDTAQRVLAMAGGSSAPKAKKGKAVTEVAGGGNGASFYKLALNAQRLRIIPSFNNGIF
                     VNIPSYQLYYFRDGNLALQSKVIVGREDRRTPVMYSKLSNVVVNPPWNVPTSIMTKDI
                     IPKLARDPGYADRASFEILDSSGNKINPRSVNWSQYLNAKSSPYRIRQKAGDDSALGR
                     YKFNMPSSDAIYLHDTPNRGLFGKTGRALSSGCVRVEKSDELATILLKEAGWGSDKKN
                     QVLGSKKTTSAQIRSDNPVYLYYVTAWVEGGKVQTLPDIYNFDAKIPQHSVNWGKLKS
                     II"
BASE COUNT          322 a          182 c          236 g          311 t
ORIGIN      
        1 tggttatata acttaggcag ttatgtacca aaatcgccct cttcagagca aatcttccaa
       61 tggactaatg ccgtgaggga aggcgattct gggcagtttg taacagacct tgttccaaga
      121 aaccatttat atcgtgatac ggcacagaga gtattggcaa tggcaggagg ctcttcagca
      181 cctaaagcca aaaaaggtaa agctgttacg gaggtagctg gtggcggtaa tggggcttct
      241 ttctataaat tagcgctcaa tgctcaacgt ttacgtatta ttccaagttt taacaatgga
      301 atttttgtga atatcccaag ctatcaatta tattatttcc gtgatggtaa tttagctctt
      361 caatctaaag tgattgttgg gcgagaagat cgccgtacac cggtaatgta tagtaagctg
      421 agcaatgtgg tagtgaatcc accttggaat gtaccgacta gtattatgac taaagatatt
      481 attcctaaac tagcgcgtga cccaggttat gcagatcgtg ctagtttcga aattcttgat
      541 agtagtggca ataaaattaa tccgcgtagc gtaaattggt cacaatattt aaatgctaaa
      601 agttcaccat accgtattcg ccagaaagca ggtgatgata gcgctttagg acgttataaa
      661 tttaatatgc caagttcgga tgcaatttat ttgcatgata cgccaaatag aggattattt
      721 ggtaaaacgg ggcgtgcgtt aagttctggt tgtgtccgtg tagaaaaatc tgatgaattg
      781 gcaaccattt tattaaaaga agcgggttgg ggatctgata aaaagaatca agtcttaggc
      841 agtaagaaga cgacctcggc tcagattcgt tcagataacc cggtttatct ctattatgtg
      901 acggcttggg ttgaaggtgg aaaagtgcaa acccttccgg acatttataa ctttgatgcc
      961 aaaatcccac aacattctgt gaattgggga aaattaaaga gcattattta atcgtgctat
     1021 ttctgataaa ttccaagaag atatcatctt c
//
LOCUS       DANLYI010000313         1040 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.315, whole genome shotgun sequence.
ACCESSION   DANLYI010000313 DANLYI010000000
VERSION     DANLYI010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1040
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.315"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            121..708
                     /locus_tag="PXD81_002498"
     CDS             121..708
                     /locus_tag="PXD81_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SocA family protein"
                     /protein_id="HDL5538474.1"
                     /translation="MFDELKIAQATAYLLHKAGGTMEHIKVMKLLYLADRLSWQRYGY
                     SISNDEYYSLPYGPVLSNTLNLMRGEEASLYQKTSIWGEWIADKEDHKISLKKSVNTE
                     DEYFFDCLSESDTEILEEVFATFGNYKPFALVEYTHNPKYIPEWEDPQGRSKPISLST
                     LLTKLGKTPSEIESILEQQAEKGTMDNLFKGLGNA"
     gene            701..1018
                     /locus_tag="PXD81_002499"
     CDS             701..1018
                     /locus_tag="PXD81_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5538475.1"
                     /translation="MLEIKRQVTILRPTNYGKEENHYKHLYILLTDPCFDGETGKGDM
                     ILSVNCSSIKDGKALDETCILKAGTHEFITRDSFIFYRHIKIDSLDEIKANIEAGYFI
                     QKP"
BASE COUNT          371 a          195 c          215 g          259 t
ORIGIN      
        1 ggtagctatc cgcaaatgag cttagtggaa caatccccac aggtatttga acaaatcgca
       61 cgtctttaaa atattaaatt aagagaaagc actatttgcg atgtagcagg agataaaaca
      121 atgtttgatg aattaaaaat agcccaagcg accgcctatt tgctacataa ggcaggcgga
      181 acaatggagc atattaaggt aatgaaattg ctttatcttg cggatcgctt atcgtggcaa
      241 agatacggct attctatttc taatgatgaa tattattcat tgccttatgg accggtatta
      301 agcaacacac tcaacctaat gcgaggagaa gaagcgagct tataccaaaa aaccagtatt
      361 tggggcgagt ggatagcaga taaggaagat cacaaaatca gccttaaaaa atccgtaaat
      421 accgaagatg aatacttctt tgattgcctt tcagagagcg acaccgaaat tttagaagag
      481 gtatttgcga cctttggaaa ttataagcca ttcgccctag tggaatatac ccataaccct
      541 aaatacattc ccgaatggga agacccacaa ggcagaagca agccgatttc tttaagcacg
      601 cttcttacta agctagggaa aacgccaagc gagatcgaaa gcattttaga gcaacaggcg
      661 gaaaaaggga caatggataa ccttttcaag ggcttaggca atgcttgaaa tcaaaagaca
      721 agtaacgatt cttagaccga ccaactacgg caaggaagag aaccactaca agcatttata
      781 tattctgctt accgatccgt gctttgatgg tgaaaccggt aaaggagata tgattttaag
      841 tgtaaactgc tcaagcatta aagacgggaa agcacttgat gaaacttgta ttttaaaggc
      901 aggaacacac gaatttatca cacgggacag cttcattttc taccgccata taaaaattga
      961 ttcgttagat gagattaagg caaatataga agccggttat tttattcaaa agccctaatt
     1021 aatgatgaag cctatcaaca
//
LOCUS       DANLYI010000314         1032 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.316, whole genome shotgun sequence.
ACCESSION   DANLYI010000314 DANLYI010000000
VERSION     DANLYI010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1032
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.316"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            <1..>1032
                     /locus_tag="PXD81_002500"
     CDS             <1..>1032
                     /locus_tag="PXD81_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5538476.1"
                     /translation="YNLNYQQISALDMYQTEKKDYELKLNTLTKELNETKLSLKQTKE
                     LLSSTQKKVLATKEQIENTLSFKLGYTLIHSTKSFKNFISLPKDLYALAIIARDRKSY
                     KTTLSSIKPKAKLTNFNKVDSKFKKKYGFKFISILDEISHTSFDSEFKLFPLNKANFE
                     SQIKGSLSLGLFIESCWKGNFGAWEYAFTSPNLQHQNAQNLLKALDVCKNRNFPIVFW
                     NKEDPMHYEKFLPISSKCDVIFTTDSNKVKDYQRDVPNAKVETLMFAANINICNPANR
                     FRYEAENICFAGSYYGVGHDDRKKQMDALLPTIIKFKGAIYDRMSQVQSADERYSYPK
                     QYRKFIRPTV"
BASE COUNT          379 a          150 c          160 g          343 t
ORIGIN      
        1 tataatctta actatcaaca aatatcagca cttgatatgt atcaaactga gaaaaaagat
       61 tatgagctta aattgaatac tttaacaaaa gaattaaatg aaaccaagct tagtttaaaa
      121 caaacaaaag agttattaag tagtacacaa aagaaagtat tagccactaa agaacagata
      181 gaaaatactc tatcttttaa attaggctat actttaattc attctacgaa gtcgtttaag
      241 aactttatct cgttgcctaa agacttatat gctcttgcta ttattgcaag agatagaaaa
      301 tcttataaaa caacattatc gtctataaag ccaaaagcta aacttactaa tttcaataaa
      361 gtagatagta aatttaagaa aaaatatggt tttaaattta tctctatttt agatgaaatc
      421 tcacatacct cttttgatag cgaatttaaa ctctttccgt taaataaagc aaattttgag
      481 agtcaaatta aaggttcttt atcattaggg ttgtttattg agagttgttg gaaagggaat
      541 tttggtgctt gggagtatgc ctttacttcg ccaaatttac agcatcaaaa tgctcaaaat
      601 ctactaaaag ctcttgatgt ttgcaaaaat agaaattttc ctattgtatt ttggaataaa
      661 gaagatccga tgcattatga gaagttttta ccaatttcat ctaaatgcga tgtgattttt
      721 actacagata gcaacaaagt gaaagattat caaagagatg ttcctaatgc taaagtggaa
      781 acattaatgt ttgctgcaaa tatcaatatt tgtaatccag caaaccgttt ccgatatgaa
      841 gctgaaaata tctgttttgc tggttcttat tatggtgttg gtcatgatga tcgtaagaag
      901 caaatggatg ctcttttgcc tactattatc aaatttaaag gagctattta tgaccgtatg
      961 tctcaggtac agtcagctga tgagcgttat tcttatccta aacaataccg caaatttatc
     1021 agaccgacag tc
//
LOCUS       DANLYI010000315          957 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.317, whole genome shotgun sequence.
ACCESSION   DANLYI010000315 DANLYI010000000
VERSION     DANLYI010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..957
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.317"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            <1..456
                     /locus_tag="PXD81_002501"
     CDS             <1..456
                     /locus_tag="PXD81_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015587011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HDL5538477.1"
                     /translation="AFAKLYTMKAAISAADMLNDKVLPFFESQGLPMLRILTDRGSEY
                     CGKVENHDYELYLAINDIEHTKTKVKHPQTNGICERFHKTILQEFYQVAFRKKIYTDL
                     TTLQADLDEWLMYYNHHRTHQGKMCCGRTPMATLLDGKRIWVEKNLSSN"
     gene            588..830
                     /locus_tag="PXD81_002502"
     CDS             588..830
                     /locus_tag="PXD81_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5538478.1"
                     /translation="MFNLNLFKRPIELSTLESWSKFCDDVGKVAFIAIPPMIYSNYDL
                     CIKIGNISALLAITYGFLLSGRTIRQFIEKEKSRWD"
BASE COUNT          314 a          167 c          197 g          279 t
ORIGIN      
        1 gcatttgcaa aactgtatac gatgaaggca gcaattagtg cagcagatat gctcaatgat
       61 aaagtcctac cgttctttga gtcccaagga ttaccgatgt tacgcatatt aactgaccga
      121 ggaagtgaat attgtggcaa ggtggaaaat cacgattatg agctttattt agcgataaat
      181 gacatagagc atacgaaaac gaaagtgaag catccccaga cgaatggtat ctgcgaacgg
      241 ttccataaaa caatcttaca agaattttac caagtggcat ttaggaagaa aatttatacg
      301 gatttaacga cattacaagc ggatttagat gagtggttaa tgtattataa tcaccatcga
      361 acacatcaag gaaaaatgtg ctgtggcaga acaccgatgg caaccttact tgatggaaaa
      421 cggatttggg tggaaaagaa tttaagctca aattaatctg acagacacgg taatttaaaa
      481 cgggggactg tcagattagg tttgatcttc tacagctttc ccgtggggag atactctccc
      541 ttcggagcat tgtgtctccc tttttattca cttaactaga ggacacaatg ttcaatctaa
      601 acttatttaa acgccctatt gagctttcaa cgcttgaatc ttggtctaaa ttctgtgatg
      661 atgtcggtaa agttgcattt attgcgatac ctccgatgat ttatagtaat tatgatctat
      721 gtatcaaaat aggaaacata agtgctttat tggcaattac ctatggattc ttattatcag
      781 gcaggactat ccgtcaattt attgagaagg agaaatcaag atgggattaa caatcggctt
      841 atggatcatt gcttttatag gctttgcact ggttttttgg gcaaatagct tactcaaaga
      901 gcaagatgaa cgccttaaac agctaaaaaa atagctcaac tttacactca atacggc
//
LOCUS       DANLYI010000316          941 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.318, whole genome shotgun sequence.
ACCESSION   DANLYI010000316 DANLYI010000000
VERSION     DANLYI010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..941
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.318"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            <1..>941
                     /locus_tag="PXD81_002503"
     CDS             <1..>941
                     /locus_tag="PXD81_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HDL5538479.1"
                     /translation="GWYLVNQAPKSRYDSENRYVHYNKSPIKLIAWGYFNGIINAATN
                     LHTVSPNLDIEKLRQFITDLRLSFPIQAPEVTNDDMHHPNEIRNLVIALNLVQDPTEH
                     IDELPKKVYPSDLFNFGKAQKTLVGSVSIIYRNVWNEIRTQHFDGEDAILKALKLVSN
                     RIYQGNSSPQSVNVFCYSKYLRSELRDFVADLVHKCITIQTGCLLQENEFSTLKVAGK
                     TWQFLFHKKTFSIKQIDEQQAVENLNSFAKTKQNAKSENEKKKEFPQQISEFASEGFL
                     QFFFEDNVDNSFNVYVVDERNNVESYYNCFGNKEEKI"
BASE COUNT          335 a          142 c          168 g          296 t
ORIGIN      
        1 ggttggtatt tagttaatca ggcacctaaa agtcgttatg actctgaaaa tcgttatgtc
       61 cattataata aaagcccgat taagcttatt gcttgggggt attttaacgg cattattaac
      121 gcagcaacta atttacatac tgttagccct aatttagata tcgagaaatt gcgacaattt
      181 atcaccgact tgcgcttatc tttcccaatt caagcgccgg aagtgaccaa tgatgacatg
      241 catcacccta atgaaattcg caatttggtt atagcgctta atttagtaca agatccaacc
      301 gaacatatcg atgaactacc aaaaaaagtt tatccaagcg atctgtttaa ttttggtaag
      361 gcacaaaaaa cgttagttgg tagtgtgagc attatttatc gaaatgtatg gaatgaaatt
      421 cgaactcaac attttgacgg tgaagatgct attttaaaag cactaaaatt ggtatccaat
      481 cgaatttatc aaggaaattc ttcacctcaa tcagttaatg ttttttgcta tagtaaatat
      541 ctacgtagtg agttacgtga ttttgtggca gatttagttc ataaatgtat tacaattcag
      601 actggttgtt tactgcaaga gaatgaattt agcactttaa aagtagcagg aaaaacctgg
      661 cagtttcttt ttcataaaaa aacatttagc attaaacaaa ttgatgaaca acaagcggtt
      721 gaaaatttaa attcttttgc aaaaaccaag caaaatgcta aaagcgaaaa tgaaaagaaa
      781 aaagaatttc cgcagcaaat tagtgaattt gccagtgaag gctttttaca gtttttcttt
      841 gaagacaatg tcgataatag ttttaatgta tatgttgtag atgaacgtaa taatgtcgaa
      901 agttactata actgtttcgg caataaagaa gagaaaatca g
//
LOCUS       DANLYI010000317          915 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.319, whole genome shotgun sequence.
ACCESSION   DANLYI010000317 DANLYI010000000
VERSION     DANLYI010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..915
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.319"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            52..900
                     /locus_tag="PXD81_002504"
     CDS             52..900
                     /locus_tag="PXD81_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HDL5538480.1"
                     /translation="MEKKALFRGFLLSTVALAVAACGNLSKVSDEGTTENPVFPKISE
                     SEFNHDGSQFGSWPNWENVRQIEKGMNKDQLYNLIGRPHFEEGLYGVREWDYAFNYRE
                     NGVHKICQFKILFDKNMNAQSFFWYPNGCNGNSSFALTGDFLFDFDKATLTTKGKEVV
                     DNVAQQLKASKAQQVKVAGYTDRLGSAAYNLDLSQRRSNTVKARLVQQGVTAQIEAVG
                     YGKANQVKACDGETGQALKDCLRPNRRVEISANCGVMKQNEGGNVAGPNGPAPLYQTP
                     AYDGSK"
BASE COUNT          309 a          146 c          184 g          276 t
ORIGIN      
        1 ttgtgaagct atttttttat acagattact ataaggaaac ttaatatgaa aatggaaaaa
       61 aaagcccttt tccgtggatt tttattatcg acagtggcat tagcagtggc tgcttgtggt
      121 aacttaagta aagtgagtga tgaaggaaca actgaaaacc cggtattccc gaaaatctca
      181 gaatctgaat ttaaccacga tggttcacaa tttggttcat ggccaaactg ggaaaacgtt
      241 cgtcaaattg aaaaagggat gaacaaagat caattatata atctaattgg tcgtcctcat
      301 tttgaagaag gcttatatgg tgttcgtgaa tgggactatg cattcaatta ccgtgaaaat
      361 ggcgtacata aaatttgcca atttaaaatt ttattcgata aaaatatgaa tgcacaaagt
      421 ttcttctggt atccaaatgg atgtaatgga aactcatcat ttgctttaac tggcgatttc
      481 ttattcgatt tcgataaagc gactttaact acaaaaggta aagaagttgt tgataatgtt
      541 gctcaacaat taaaagcatc taaagcacaa caagttaaag tagcaggcta tacagaccgt
      601 ttaggctctg ctgcttataa cttagactta tcacagcgtc gttctaatac agttaaagct
      661 cgtttagtac aacaaggcgt tacagcacaa attgaagcgg taggttatgg taaagcgaat
      721 caagtaaaag cttgtgatgg tgaaactggt caagcattaa aagattgttt acgccctaac
      781 cgtcgtgttg aaatttctgc aaattgtggt gtaatgaaac aaaatgaagg tggcaatgtt
      841 gcaggtccta atggtccggc tccactttat caaaccccag catatgatgg cagtaaataa
      901 tttctagttt tgact
//
LOCUS       DANLYI010000318          907 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.320, whole genome shotgun sequence.
ACCESSION   DANLYI010000318 DANLYI010000000
VERSION     DANLYI010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 907)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 907)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..907
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.320"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            14..>907
                     /locus_tag="PXD81_002505"
     CDS             14..>907
                     /locus_tag="PXD81_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005824742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDL5538481.1"
                     /translation="MNSKFSWLTLNNKSDSILFVESTWQGYKNRWKYKIASYPDHPKR
                     TNQKLLRLVEKAKDKGIPTVFWNKEDSIHFDRFIDSAKHFDHIFTVDQNAIARYREVV
                     PDTTTVNVGMFPVQPRIHNFQGFNFKHLAANFVGSYSHHIHNKRRERQDMLFGAALKA
                     GLPVTIFDRNSDRKSENYRYPKNSFNLNVQPAVSYTETANIYRDYLVSLNINTVEDSP
                     TMFSRRVVEILACGGILVSTPSMAIDNIFKEYCHIVQSEDEAIELFQRLKRGASRDDL
                     NKAIAGSEFVLSNFTWEHFLSQ"
BASE COUNT          297 a          154 c          165 g          291 t
ORIGIN      
        1 ttcactagaa ggcatcaact ctaaattctc ctggttgacg ttgaataaca aatcagattc
       61 aatattattt gtagaatcta cttggcaagg ctacaaaaac cgttggaaat acaaaatagc
      121 atcttatcct gaccacccca aacgaaccaa tcaaaaattg ttgcgtttgg tggaaaaagc
      181 aaaagataaa ggtattccaa cggtattttg gaacaaggaa gattccatac atttcgaccg
      241 atttattgat tcagcgaagc attttgatca tatatttact gtagatcaaa atgctattgc
      301 tcgttataga gaggttgtac ctgatacaac cactgtcaat gtaggtatgt ttcctgttca
      361 gccaaggatt cataattttc aaggatttaa ttttaagcac cttgccgcaa attttgtagg
      421 gagttacagt catcatatcc acaataagcg tagagaaaga caggatatgt tgtttggtgc
      481 agcacttaaa gcaggtcttc cggtaaccat ctttgataga aactctgata gaaaatcgga
      541 aaattatcgt tacccaaaaa atagctttaa tttaaatgtt caacctgctg ttagttatac
      601 tgaaactgct aatatttatc gtgattatct tgtttctttg aatattaata ctgtcgaaga
      661 ttcccctacg atgttttctc gcagagtcgt tgaaatatta gcttgtggag gaatattagt
      721 ctctacacca agtatggcta tcgacaatat atttaaagaa tattgtcata tcgtacaaag
      781 tgaagatgag gctatagaat tattccaacg tttaaaacga ggtgcttctc gtgatgattt
      841 gaataaagcg attgcaggct ctgaatttgt attgagtaac tttacttggg aacatttctt
      901 atcccaa
//
LOCUS       DANLYI010000319          893 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.321, whole genome shotgun sequence.
ACCESSION   DANLYI010000319 DANLYI010000000
VERSION     DANLYI010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 893)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..893
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.321"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            12..638
                     /locus_tag="PXD81_002506"
     CDS             12..638
                     /locus_tag="PXD81_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HDL5538482.1"
                     /translation="MTKIKFYGNLKKFGSEFSLEVKDTAEAIRALCTQISGLREALRD
                     GVYKVRIGKQYLDPSALEKGLFYCLKKGQTIHFTPVIKGAKSGGVFNFVLGAALIGAA
                     FFTGGASIAAWGAGAKMMGMLGASMLLGGVSQMLTKMPKAPTMGNETEKESSTAFSNL
                     NNLVTQGKPVPLAYGLIRTGSLVISQGVETITIKENQPASNKKTGFRK"
     gene            687..>893
                     /locus_tag="PXD81_002507"
     CDS             687..>893
                     /locus_tag="PXD81_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL5538483.1"
                     /translation="MNLTKAESNLNAKITEEKKARVDADKVNADKLTDITSRVANAES
                     TITNFQSTKANKSEVASLAQSSLQA"
BASE COUNT          297 a          167 c          206 g          223 t
ORIGIN      
        1 aatggtgagt catgactaaa attaaatttt acggcaattt aaaaaaattt ggtagtgaat
       61 ttagcttaga agttaaagat actgccgaag ctatccgagc cttatgtaca cagattagcg
      121 gattgcgtga ggcattacga gacggtgttt ataaagtccg tatcggcaaa cagtatttag
      181 acccgtcagc ccttgaaaaa gggctttttt attgtctgaa gaaagggcaa accattcatt
      241 ttacccctgt tatcaaagga gcgaaaagcg gtggcgtgtt taattttgtt ttaggtgcag
      301 ccttgatcgg agccgcattt tttactggcg gtgcttctat tgcggcttgg ggagctggag
      361 caaaaatgat gggtatgtta ggggcttcga tgttactagg aggagtttcc caaatgctca
      421 ccaaaatgcc aaaagccccc acaatgggta atgaaacaga aaaagaaagc tcaacggctt
      481 tttcaaattt aaataacttg gtgactcaag gcaaacctgt gccgttggct tatggcttaa
      541 ttcggaccgg ctcgcttgtg atatcgcagg gagttgagac gatcaccatt aaagaaaacc
      601 aaccggcaag caacaagaaa acaggcttta gaaaataagg gataattacc gattcagcac
      661 aagcacagca acttaatcag ttatctgtga acttgaccaa agcagagagc aatttaaatg
      721 ccaaaatcac ggaagaaaaa aaagctcgtg ttgatgcaga taaagtgaat gcagataaac
      781 tcacagacat aacaagccgt gttgcaaatg cggaatcaac tattaccaat ttccaatcaa
      841 ccaaagcgaa taaaagtgag gttgcgagcc ttgcacaaag ttcgttgcag gca
//
LOCUS       DANLYI010000320          883 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.322, whole genome shotgun sequence.
ACCESSION   DANLYI010000320 DANLYI010000000
VERSION     DANLYI010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..883
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.322"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            complement(15..245)
                     /locus_tag="PXD81_002508"
     CDS             complement(15..245)
                     /locus_tag="PXD81_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005720191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5538484.1"
                     /translation="MALHRCPECRKRISDSLEHCIHCGFSFKEADLAIYRQKLEQRRL
                     HNQEINRKSVKLQLIWLVIFIAVIALASWITN"
     gene            complement(253..>883)
                     /locus_tag="PXD81_002509"
     CDS             complement(253..>883)
                     /locus_tag="PXD81_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5538485.1"
                     /translation="GYPWWHVEIGNASMARSLAYVFGTSVILFLCYALGYSLLNKVFQ
                     LHYFRNLGWLRVIVLVSVFTVPVILTFYLFTGIVPIYISFIYIFVSGVSVFFFRKRWN
                     NTKFDLDFRKMLLGGSFALFNIFIFLYFSLLSLHIGYLRAGLRTTYDYIEIENQKYYI
                     LSTNSDNGYIMGTKIRGNQSFLFFNRDTHQYYHVHIEQCTDSRLCKVIV"
BASE COUNT          308 a          175 c          149 g          251 t
ORIGIN      
        1 accgggcttt tatttcaatt ggttatccaa cttgcgagag caattaccgc aataaaaata
       61 actaaccaaa taagttgtaa ttttacgctt ttgcggttga tctcctggtt gtgtaatcgg
      121 cgctgttcta acttttgccg atatatcgct aaatcggctt ctttaaacga gaagccacaa
      181 tgtatgcaat gttctaaact gtcgctaata cgctttcgac actccggaca acggtgtaat
      241 gccataatta cctcaaacta ttactttgca taatcggcta tccgtacatt gttcaatatg
      301 aacatggtag tattgatgag tatcacgatt gaaaaacaag aaagattgat tacctcttat
      361 ttttgttccc ataatgtagc cgttatcact gttggtggat aatatgtagt atttttgatt
      421 ctctatttcg atgtaatcat aggtagttct tagtccggca cgcaaataac caatatgcaa
      481 tgagagcaaa ctgaaataga gaaaaataaa gatgttaaac agggcaaaac ttcctcctaa
      541 tagcatcttt ctaaagtcca gatcaaactt ggtattattc catctcttgc ggaagaagaa
      601 tacgctcaca ccacttacaa aaatatagat aaaagagata taaatgggca ctatcccggt
      661 aaataagtaa aaagtaagaa taacaggcac tgtaaacaca gaaaccaaca caatcaccct
      721 taaccaacct agatttcgga aataatgcag ctgaaaaact ttatttagta aactataacc
      781 tagtgcataa cacaagaata agatgacgga agtaccgaat acataagcca aacttcttgc
      841 catactggca ttgccgattt caacatgcca ccaagggtag cca
//
LOCUS       DANLYI010000321          879 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.323, whole genome shotgun sequence.
ACCESSION   DANLYI010000321 DANLYI010000000
VERSION     DANLYI010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..879
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.323"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            <1..626
                     /locus_tag="PXD81_002510"
     CDS             <1..626
                     /locus_tag="PXD81_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HDL5538486.1"
                     /translation="NTVLNTLQCDYVLFSSEDNYQVIINLKKADYPKNEANFITMTLN
                     KKFGDAKFSGANHYLRCASFFNKKSTNNNEKSVLVDFTNTKTEEDNKCYFDNLLSSYK
                     NNNVKLEPLDIKIIDELGDDKAVIAQKEIQAEIALCKRIFKQLDWSAVDFRIVKRLYR
                     KGFSENEIAVALVRFTDFEDRHCDSHDYLTRTITKAIQNYQQCSKAC"
BASE COUNT          354 a          130 c          139 g          256 t
ORIGIN      
        1 taaacacagt tttaaacaca ctacaatgcg attatgtgct gttttcttct gaagacaact
       61 accaagtcat catcaatcta aaaaaagctg attaccccaa aaatgaagca aatttcatta
      121 caatgacttt aaacaagaaa tttggtgatg caaaattttc cggtgcaaac cattacttaa
      181 gatgtgccag cttcttcaac aagaaaagca caaacaacaa tgaaaaatca gttttagttg
      241 attttacaaa cacaaagaca gaagaagaca ataaatgtta ttttgataac ttattatcta
      301 gttataaaaa taacaatgta aaacttgagc ctttagacat caaaattatt gatgagcttg
      361 gagatgataa agcagttatt gcacaaaaag aaattcaagc agaaattgct ttgtgtaaac
      421 gtatattcaa acaattagat tggtctgctg ttgattttag aattgttaag cgtctgtaca
      481 ggaaaggatt ttcagaaaat gaaattgctg ttgcacttgt cagatttact gattttgaag
      541 acagacattg tgattcacat gattatttga caagaacaat cacaaaagcc attcaaaact
      601 atcaacaatg ctcaaaagct tgttagatgt tataaggaaa gttaaaaatc aacaaaagat
      661 ttgacaaatt tatataaaat ccttattata tatgaaaagg aagcgtagcc ttaaaacgca
      721 aatgtgggtt agcacatggt caatctgaaa ctaatctagg atatttaagg tctagcagat
      781 ggcaagcatc agaaaaactt gaaactaatc taggatattt aaggcctagc agatggcaga
      841 catcagaaaa actaacactt aaattttata tttattaag
//
LOCUS       DANLYI010000322          879 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.324, whole genome shotgun sequence.
ACCESSION   DANLYI010000322 DANLYI010000000
VERSION     DANLYI010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..879
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.324"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            <1..202
                     /locus_tag="PXD81_002511"
     CDS             <1..202
                     /locus_tag="PXD81_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CMP-N-acetylneuraminate-beta-galactosamide-
                     alpha-2, 3-sialyltransferase"
                     /protein_id="HDL5538487.1"
                     /translation="EDYIFQEFSHKKCRVYTYFSSAVINILNKSNHIEVVALRVNVDN
                     PAYIESYELLEKLGVQIVDIRE"
     gene            204..>879
                     /locus_tag="PXD81_002512"
     CDS             204..>879
                     /locus_tag="PXD81_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 25 protein"
                     /protein_id="HDL5538488.1"
                     /translation="MKKYLISLEKDVKRRELFFSQPNTEDFEVFSAINTMSSDWEALN
                     NHFDLALFEQRYGRKTTKGEVGCTLSHLAVYQKIAEDSTIAEDDYCLVCEDDALFADD
                     FQKHLDALLAQNLKVDIILVGQSKIPTFNDSELELNYPSTFGFLQKNIPNSQYKYAYS
                     YLNYFAGTVAYLIKKSTACQFLAQCAGKNPYWLADDFILFEKNFKFDVLIVRPLMAIE
                     NPVLISN"
BASE COUNT          296 a          122 c          166 g          295 t
ORIGIN      
        1 tgaagattat attttccaag aatttagcca taaaaaatgt agagtctata cttattttag
       61 tagtgcggtg atcaatattt taaataaaag taaccatatt gaagtggtag cattaagagt
      121 gaatgtagat aatcctgctt atattgagag ctatgaattg cttgaaaaat taggcgttca
      181 gattgttgat attagagaat aaaatgaaga aatatttaat ttcattagaa aaagatgtaa
      241 aaagacgaga gctatttttt agccagccta atacggaaga ttttgaggtg ttcagtgcca
      301 ttaatacaat gagtagtgat tgggaggcat taaataacca ttttgatttg gctctgtttg
      361 aacagcgata tgggcgtaaa accactaagg gcgaagtagg ttgtacttta agccatttag
      421 ccgtttacca aaaaattgcc gaagatagca ctattgcgga agatgattac tgcctagtct
      481 gtgaagatga tgccttattt gccgatgatt tccaaaaaca tcttgatgct ttattagcac
      541 aaaatttaaa agtcgatatt attttagttg ggcagtcgaa aattccaact tttaacgata
      601 gcgaattaga gcttaattac ccatcaacct ttggcttttt gcaaaaaaat attccgaata
      661 gccaatataa atatgcttat tcttacctga attattttgc tggtacggtt gcatatttaa
      721 ttaaaaaatc gaccgcttgt cagtttttag cacaatgtgc cggaaaaaat ccatactggc
      781 ttgccgatga ttttatttta tttgaaaaga atttcaaatt tgatgtgtta attgtccgcc
      841 cgttaatggc gatagaaaat cctgttctaa ttagtaatt
//
LOCUS       DANLYI010000323          855 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.325, whole genome shotgun sequence.
ACCESSION   DANLYI010000323 DANLYI010000000
VERSION     DANLYI010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 855)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..855
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.325"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            <1..638
                     /locus_tag="PXD81_002513"
     CDS             <1..638
                     /locus_tag="PXD81_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HDL5538489.1"
                     /translation="ITDQMTKSKNLWLKYEEEAKTTLKPYQEETISRVIQDEINKKMA
                     EIGTTTLEIGGKEHELEDLENQINALALTDEEKLAEDVENDLISKLDEAKAALAKNQQ
                     TLKEKRDELLGLNNKLDSSPLVKKGKNALAEGTNAFASGENAIAFGTDSQATGNNAIA
                     LGANSKANAESAIAIGKGAQALKEKALALGENAIANRASAIAIGDNHSSKL"
     gene            complement(692..>855)
                     /locus_tag="PXD81_002514"
     CDS             complement(692..>855)
                     /locus_tag="PXD81_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015587011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS481 family transposase"
                     /protein_id="HDL5538490.1"
                     /translation="YTDLTTLQADLDEWLMYYNHHRTHQGKMCCGRTPMATLLDGKRI
                     WVEKNLSSN"
BASE COUNT          320 a          160 c          158 g          217 t
ORIGIN      
        1 aaattacaga tcaaatgaca aaatccaaaa atctatggtt aaaatatgaa gaggaagcca
       61 aaacaacatt aaaaccatac caagaagaaa caatatctag ggttatacaa gatgaaatta
      121 ataaaaaaat ggctgaaata ggtactacaa cactagagat tggtggtaaa gaacacgagc
      181 tcgaggatct tgaaaaccaa attaatgctt tagctttaac tgatgaagaa aaacttgcgg
      241 aagatgtgga aaatgattta atctcaaagc ttgatgaggc taaagcagcc ttagcaaaaa
      301 accagcaaac gctcaaagaa aagcgtgatg aattactagg gctaaataac aaattagata
      361 gctcaccact tgttaaaaaa ggtaaaaatg cacttgcaga aggcacgaat gcctttgctt
      421 ctggtgaaaa tgccatcgca ttcggcacgg attcacaagc tacaggtaat aacgctatcg
      481 cactaggtgc aaattcaaaa gcaaacgcag aaagtgcgat tgcaattggt aaaggtgcac
      541 aagcattaaa agaaaaagcc cttgctttag gcgaaaatgc cattgccaac agagcatctg
      601 ctattgcgat tggtgataac cattcatcta aattgtagaa gatcaaacct aatctgacag
      661 tcccccgttt taaattaccg tgtctgtcag attaatttga gcttaaattc ttttccaccc
      721 aaatccgttt tccatcaagt aaggttgcca tcggtgttct gccacagcac atttttcctt
      781 gatgtgttcg atggtgatta taatacatta accactcatc taaatccgct tgtaatgtcg
      841 ttaaatccgt ataaa
//
LOCUS       DANLYI010000324          853 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.326, whole genome shotgun sequence.
ACCESSION   DANLYI010000324 DANLYI010000000
VERSION     DANLYI010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..853
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.326"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            141..>853
                     /locus_tag="PXD81_002515"
     CDS             141..>853
                     /locus_tag="PXD81_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HDL5538491.1"
                     /translation="MLNWAGKSTAYQTLQTPTSKTLTPCEEESVDFENTQNIFIEGEN
                     LDVLKALQKSYFNSVKMIYIDPPYNTGNDFIYNDSFADTKADYAEKVGDVDEHGKLKR
                     AFVRNSKENGHYHSNWLNMMLPRLHLAKNLLRDDGVIFISIDDNEQAQLKLLCDEVFG
                     EENFVAILSVENNPKGRKNSKFISVSNDFCIIYAKNKEMGKFVENIPKNAKDMCQDEN
                     GHYVHASGKRVLVGENKFN"
BASE COUNT          296 a          144 c          164 g          249 t
ORIGIN      
        1 ttgccaactt ttcgcttaaa gatcaacaac tttctcaatt aaaatccctt ttacctgaaa
       61 ttttcaccga aggtaaaatc gacttcgaca aattccaaca acttttttcg gataattttt
      121 ccaccgaacc tgaccgctat atgctcaact gggcaggaaa atcaacagcc tatcaaacct
      181 tacaaacccc aaccagcaaa acgctcactc cttgcgagga agagagtgtg gattttgaaa
      241 atacgcagaa tatctttatt gagggtgaaa atttagatgt attaaaagcc ctgcaaaaat
      301 cctacttcaa ttcggtcaaa atgatttata tcgatccgcc atacaataca ggcaacgatt
      361 ttatttataa cgacagtttt gccgatacca aagcagatta tgcggagaaa gtgggcgacg
      421 ttgatgaaca cggcaaattg aaacgagcct ttgttcgtaa cagcaaagaa aatgggcatt
      481 atcacagtaa ctggctgaat atgatgttgc ctcgtttgca tctcgcaaaa aatttactgc
      541 gtgatgacgg ggtgattttt atttcaattg atgataacga acaggcacag ctgaaattgc
      601 tttgtgatga agtgtttggg gaggagaatt ttgttgccat attatcagtt gaaaataatc
      661 cgaaaggtag aaagaattct aaatttattt ctgtaagtaa tgatttttgt attatttatg
      721 ctaaaaataa agaaatgggg aaatttgttg aaaatattcc taaaaatgct aaggatatgt
      781 gtcaagatga aaatggacat tatgttcacg caagtggaaa acgagtatta gttggggaaa
      841 acaaatttaa tca
//
LOCUS       DANLYI010000325          849 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.327, whole genome shotgun sequence.
ACCESSION   DANLYI010000325 DANLYI010000000
VERSION     DANLYI010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..849
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.327"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            <1..301
                     /locus_tag="PXD81_002516"
     CDS             <1..301
                     /locus_tag="PXD81_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS1595-like element ISMha2 family transposase"
                     /protein_id="HDL5538492.1"
                     /translation="KVKPDSLVYADFYRSYDVLDVSEFNHFRINHSTHFAENHNHING
                     IENFWSQAKRHLRKFNGIPKAHFELYLKECEWRFNHSDLKSQIFLLKQLVKGGLG"
     gene            complement(500..739)
                     /locus_tag="PXD81_002517"
                     /pseudo
     CDS             complement(500..739)
                     /locus_tag="PXD81_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651213.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
BASE COUNT          271 a          161 c          141 g          276 t
ORIGIN      
        1 gaaagtgaag ccagacagcc ttgtttacgc cgatttttat cgaagctatg atgtgcttga
       61 tgtgagtgaa tttaaccatt ttcgtatcaa tcacagcaca cacttcgctg aaaatcacaa
      121 tcacattaac ggaattgaga atttttggag tcaagcaaaa cgtcatttac gcaagtttaa
      181 tggtattccg aaagcccatt ttgagctata tttgaaagaa tgcgaatggc gttttaatca
      241 cagtgactta aaatctcaaa tattcctttt aaaacaattg gttaaagggg ggttaggtta
      301 gttatctagg acagccccaa aaataatggc ataagtgact cacttatgcc attttttata
      361 tgctttaacg tttattaaaa atcttaaaaa tattgttggc ttcctctaaa taatgcaaga
      421 ttttccgcac tcatattgcc gttaccaaca cctgctacga caataccttg atagcctaca
      481 tctaacaagg ctttagtggg ccaattttca caatctgctc acctttgata ttcgccaatg
      541 ttttcatttc tggcactgct tcaattaagg tttcgatatt caattgccct gcttgataag
      601 cactactatt tgcattctgc tgactaccag caatcgttcc gcctgtcgct aaaatagtga
      661 tattaggtaa ttctgccgca ttagctgtta caaataagcc acctaacatt gctgctaatg
      721 ctaatttttt gaatttcata taaggctcct taattaaaaa aactaaagac agataatacc
      781 gataaatcaa tgtaaattat ttgatctgat acataaaagc gggaattaat tactgggtag
      841 aaataggtt
//
LOCUS       DANLYI010000326          830 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.328, whole genome shotgun sequence.
ACCESSION   DANLYI010000326 DANLYI010000000
VERSION     DANLYI010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..830
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.328"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            153..797
                     /locus_tag="PXD81_002518"
     CDS             153..797
                     /locus_tag="PXD81_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDL5538493.1"
                     /translation="MFGHIWQYAREWGYTTLASPNTGMKRFPIRKREIYVENNIFEMV
                     YEVADQDIKDLMDLAYLTGQRPVDLVNIQRDHIFDGYLHIVQQKTQAKLRIEIVGKLA
                     DIIQSRLEKTTRNFLFTTSKGEKMTSVKLGRRFTYIREQAAKKHPEYANEIKEFQFRD
                     LRAKSGTDKALAQGEEAARQQLGHTSVQMTKVYIRKAPIVTPIQESVSSQKHKP"
BASE COUNT          330 a          163 c          141 g          196 t
ORIGIN      
        1 aaacacttta ctaaatgttt aatatttact aaactacacc catcaaaaca aacaacgcca
       61 cagacaaagg ggaaaccaaa atgaacgcac aaacaacgct aaacaaccac aaagactaca
      121 ttcactaaga atttttatca aagccctttt caatgttcgg tcatatttgg caatacgcaa
      181 gagagtgggg ttacacaacg ttagctagtc ctaatacagg aatgaaacgc tttccaatta
      241 gaaaacgtga aatttacgtt gagaacaata tttttgaaat ggtgtatgaa gtagcagatc
      301 aggatattaa agatttaatg gatctcgctt accttacagg acaaagacct gtagatttag
      361 ttaatatcca acgcgatcat atatttgatg gatatctcca tattgtgcaa caaaaaacac
      421 aagccaaact gcggattgag atagttggca aacttgctga tataattcaa agtaggttag
      481 aaaaaaccac aaggaatttc ttatttacaa cgtcaaaagg agaaaaaatg acctctgtaa
      541 aattaggaag acgctttact tatatccgag aacaagcagc aaaaaaacat cccgaatacg
      601 caaatgaaat aaaggaattc caatttagag atctaagggc taagtcaggg acagacaaag
      661 cccttgcaca aggagaagaa gctgcacgcc aacaattagg tcatactagc gttcaaatga
      721 caaaagtcta tatccgaaaa gccccgatag taaccccaat tcaagaaagc gtttcctccc
      781 aaaaacacaa accttaaaac ctagtttcgg aacatctgag aatttttcgg
//
LOCUS       DANLYI010000327          792 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.329, whole genome shotgun sequence.
ACCESSION   DANLYI010000327 DANLYI010000000
VERSION     DANLYI010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..792
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.329"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            complement(<1..668)
                     /locus_tag="PXD81_002519"
     CDS             complement(<1..668)
                     /locus_tag="PXD81_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4189 domain-containing protein"
                     /protein_id="HDL5538494.1"
                     /translation="MFMVIQLALYNLQTYEVGRNATTIFMTTDKVYTGGSTWTYLFAD
                     ADNKITSVREMNGFGDVEKEFKCGEYKEAVTIASKEDLAKRKTQPRLWSSIYMSPEQN
                     MMWVTDNTSKNLENAKQDAFKQCEKNGGRNCQMILSFSNMCLAFAQGYKDGQGFEDFG
                     YGIVSSLAEENALENCNQKGGSSCLLSAPAVCAIPCDILSNNKCMYDQPQIIIPGKNN
                     NQPI"
BASE COUNT          254 a          168 c          115 g          255 t
ORIGIN      
        1 ttaatcggtt ggttattatt cttacctgga ataataattt gaggctgatc gtacatacat
       61 ttgttgttcg acaatatatc acagggaata gcacatacgg caggggcaga aagaagacaa
      121 cttgaaccac ctttttgatt acaattctct aatgcatttt cttcagcaag actactaacg
      181 ataccatacc caaaatcctc gaagccttga ccatctttat aaccttgtgc aaaagcaagg
      241 cacatattac taaacgataa gatcatttgg caattacgac caccgttctt ttcacactgt
      301 ttaaacgcat cttgcttcgc attttctaaa tttttagacg tattatcagt cacccacatc
      361 atattttgtt caggtgacat ataaattgat gaccataaac gaggctgtgt tttacgcttt
      421 gctaagtcct ctttactggc aatggttacc gcttctttat attcaccaca tttaaactct
      481 ttttcgacat caccaaaacc attcatttca cgaacagaag tgattttatt atcagcgtca
      541 gcaaaaagat atgtccaagt cgatccacct gtataaacct tatctgttgt cataaatata
      601 gtggttgcat tacgaccaac ttcataagtt tgcaaattat acaaagctag ttgaataacc
      661 ataaacatag cttaattcat tatcttttac gcattgaacc gctgtatcag gtaaacgttt
      721 acgtgcaaaa aattcagata catctttacc acgccaataa acactatcta gtggcttaat
      781 cattgtagaa ca
//
LOCUS       DANLYI010000328          790 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.330, whole genome shotgun sequence.
ACCESSION   DANLYI010000328 DANLYI010000000
VERSION     DANLYI010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..790
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.330"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            <1..>790
                     /locus_tag="PXD81_002520"
     CDS             <1..>790
                     /locus_tag="PXD81_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5538495.1"
                     /translation="ASIAQQNLQSIWRTDAQSAVDALKIGGANLLVDSEYLTTARWGG
                     SSRVASSQYGDRRLTQVFVTQAGTGHFGVTQGTQKATTRIRQGETYTLSLNAQGTAGF
                     TRTGLNYVYLIREDGGNFRLPTLPLTASLSQRPKVTFTAPWTSNQVRLLIGANGIFEA
                     TDWFAFHSVKLEMGNVATGWTPTAKDIDDKVSAVQSNLTAYQAAQAKADQAKATQISG
                     LTTRMGAAESNLTRTERAVTELNQTTVTTLRDLTARTKTTEGSLS"
BASE COUNT          212 a          198 c          210 g          170 t
ORIGIN      
        1 gcaagtattg cccagcagaa cttacagagc atttggcgca ccgatgcgca aagtgcggtg
       61 gatgccttga aaatcggtgg tgcaaatcta ctggttgata gtgagtattt gacgacagcc
      121 cgttggggcg gtagctcacg agtggcaagc tcgcaatatg gtgatcgtcg cttgactcaa
      181 gtgtttgtga cccaagcagg cacagggcat tttggtgtga ctcaaggcac acaaaaagcc
      241 actacgcgta ttcgtcaggg tgaaacctat accttgtcac taaatgcgca ggggacggca
      301 ggttttaccc gcacagggtt aaactatgtg taccttatcc gcgaagatgg tggcaatttt
      361 agattgccaa ctttgccgct tactgcatca ttgtcccaac gccctaaagt gacatttact
      421 gcgccgtgga cgagcaatca agttcggtta ctgattggcg caaatggcat atttgaggcg
      481 acagactggt tcgcatttca cagcgttaag ctcgaaatgg gtaatgttgc cacaggctgg
      541 acaccaaccg ccaaggatat tgacgacaaa gtatcagcgg ttcagtccaa cctcaccgct
      601 tatcaggcgg cacaagctaa agcagatcaa gccaaagcta cgcagatttc ggggctcaca
      661 acccgaatgg gggcagcgga aagtaacctc actcgcaccg aacgggcagt gacggaactt
      721 aatcagacaa cggtgacgac actgcgtgat ctaaccgcgc gcaccaaaac aacggaaggt
      781 tcgctctctc
//
LOCUS       DANLYI010000329          760 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.331, whole genome shotgun sequence.
ACCESSION   DANLYI010000329 DANLYI010000000
VERSION     DANLYI010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..760
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.331"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            <1..713
                     /locus_tag="PXD81_002521"
     CDS             <1..713
                     /locus_tag="PXD81_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HDL5538496.1"
                     /translation="HNKFKAKIALQGAQLLSWQPYNAEQDVLWLSEIEPFRIGSAIRG
                     GIPICYPWFGSIKEPAHGTARIQEWRLVEHTCSDENVGLVFALENKAKMEMMLGETCE
                     LYFTHLDPEPAQLALHSYFNIGDIEKVEVQGLPTRCFDKLTDQEVEVPSPRKISENVD
                     CIYPAQAVNFIQDFANQRTIKVEHINATETVLWNPWHNTVSAMTETAYQNMVCVETAR
                     INTLLQQNETLGVRVLVR"
BASE COUNT          241 a          127 c          175 g          217 t
ORIGIN      
        1 atcacaataa attcaaagct aaaattgcat tacaaggagc acaattgcta agttggcagc
       61 cgtataatgc agagcaagat gtgctgtggt taagtgagat agaaccgttt agaattggct
      121 ctgcgattcg tggcggtata ccaatttgtt atccgtggtt cggttcaata aaagagcctg
      181 cccacggcac agctcgcatt caagaatggc gtttagttga gcatacttgt tctgatgaaa
      241 atgtcggttt ggtatttgcc ttagagaata aagcgaaaat ggaaatgatg ttaggcgaaa
      301 cttgtgagtt atattttacc catttagatc cagaaccagc acaactggca ttgcactctt
      361 attttaatat tggtgatatt gaaaaagtgg aagtgcaagg cttgccaact cgctgttttg
      421 ataagctaac cgatcaagaa gttgaagtgc catcacctcg caagataagt gaaaatgtgg
      481 attgtattta tccggcacaa gcggtgaatt ttattcaaga ttttgcaaat cagcgcacca
      541 ttaaagttga gcatattaat gcgaccgaaa ccgtactttg gaatccttgg cataataccg
      601 ttagtgcgat gacagaaacg gcgtatcaaa atatggtatg tgtggaaacc gctcggataa
      661 atactctact tcaacaaaat gaaacgctag gagtgagggt tttagtgcga tagtgtttaa
      721 tgagagcaag cagctgaatt agtaatattg tttacaaatt
//
LOCUS       DANLYI010000330          724 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.332, whole genome shotgun sequence.
ACCESSION   DANLYI010000330 DANLYI010000000
VERSION     DANLYI010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..724
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.332"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            18..668
                     /locus_tag="PXD81_002522"
     CDS             18..668
                     /locus_tag="PXD81_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017806412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HDL5538497.1"
                     /translation="MILGGALLTGTAVAHQAGDVIFRAGAVHVKANSSSDAKSKAATG
                     IDVDLEVKNNTQLGLTATYMLADNVGIELLGATPFSHKINAKAKVLESGAEVDLDRVV
                     ILKQLPPSLYAQYYFFELTAKVKPYVGAGLNYTRFYHAKSVSPAVTDLSVKKHSFGPV
                     ANLGVDVKLTDNIYFNAAAWYTRIKTTAKFKAAGADHEVKVKLDPVVLFAGFGVKF"
BASE COUNT          229 a          128 c          152 g          215 t
ORIGIN      
        1 aaaaatcaac tttagcaatt attttaggtg gggcattatt aactggaact gcggtggcac
       61 atcaagcagg tgatgtgata ttccgtgccg gtgcggtaca tgttaaggca aattcaagtt
      121 cagatgcaaa atcaaaagca gcgacaggta ttgatgtaga tttagaagtg aaaaacaata
      181 ctcagttagg cttaactgca acttatatgt tagccgataa tgtgggtatt gagttattag
      241 gtgcaacccc tttctctcat aaaattaatg ccaaagccaa agttttagaa tcaggtgcag
      301 aagtagattt ggatagagtt gttatcttaa aacaattacc accgagcctt tatgcccaat
      361 actatttctt tgagctaact gcaaaagtaa aaccttacgt tggtgcaggt ttaaattaca
      421 ctcgcttcta ccacgctaaa tcagttagcc ctgctgttac tgacttaagt gtaaaaaaac
      481 attctttcgg accggtagca aacctaggtg ttgatgtaaa attaaccgat aacatctact
      541 tcaacgcggc agcttggtac acacgcatta aaacaaccgc aaaatttaaa gcagcaggtg
      601 cagatcacga agtaaaagta aaacttgatc cggttgtctt atttgccggt ttcggggtga
      661 agttctaatt atttgttggg aacgagttgg gctaatctta gtgattagtc cttttttgtt
      721 aaag
//
LOCUS       DANLYI010000331          722 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.333, whole genome shotgun sequence.
ACCESSION   DANLYI010000331 DANLYI010000000
VERSION     DANLYI010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 722)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 722)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..722
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.333"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            complement(200..>722)
                     /locus_tag="PXD81_002523"
     CDS             complement(200..>722)
                     /locus_tag="PXD81_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5538498.1"
                     /translation="IEYQKEVCNKFNFSEKGKYLIEEYIPDIYGDLIPRDFKFFIFQG
                     EIAIIMEVNRNKGVIQKHRFYEPDFTTIKNGKVIGLTPEFQLLRLPKPEFADALINLA
                     KRVSIALPTPFARVDLYWDGKQAKVGEITLTPFYGYPYKWTLSQDIIMGRMWLEALDR
                     MGKKLEDSFDWGN"
BASE COUNT          252 a          133 c           99 g          238 t
ORIGIN      
        1 tgtgattgat aataaattta cataagattg tgcgattata taaataagga ttgttcctaa
       61 aataatagca agagaagagc ttaatagagt accttttgaa agaaaaaggt ctccaggcat
      121 cacataagcc ccccaaccaa taatagcacc aaaactaatc gctatagcct gaaagaacga
      181 tacctttgtt gatttcacat taatttcccc aatcgaaaga atcttccaat tttttcccca
      241 ttctatctag tgcctccaac cacattcttc ccattatgat atcctgtgat aatgtccatt
      301 tgtatggata accatagaaa ggagttaatg taatttctcc aactttagct tgctttccat
      361 cccaatataa atccactcga gcaaatgggg taggtaatgc aatagatact cgttttgcta
      421 gattaatcaa agcatcggca aattcaggtt taggtaaacg taataattgg aattctggag
      481 ttaagccaat aacttttcca ttcttaatag tggtaaaatc aggctcgtaa aatcgatgtt
      541 tttgaattac acctttatta cgatttactt ccataataat tgcaatttca ccctggaata
      601 taaaaaattt aaaatctctc ggaattaaat caccataaat atcagggata tattcttcaa
      661 taagatattt ccctttttct gaaaaattga atttattaca aacttccttt tgatattcaa
      721 ta
//
LOCUS       DANLYI010000332          710 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.334, whole genome shotgun sequence.
ACCESSION   DANLYI010000332 DANLYI010000000
VERSION     DANLYI010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..710
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.334"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            <1..663
                     /locus_tag="PXD81_002524"
     CDS             <1..663
                     /locus_tag="PXD81_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005714533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL5538499.1"
                     /translation="PEIRRKKLTEWFNTRDIPEKEKSYISQLKSGKSSFGERAARRLE
                     NEYGMPSFYLDYDEETESAIVSAMSIQETQSHQYPIHLIDFKAKAGETGFVNTNYPEI
                     IQSIYFSLDGLLEIVGRKSSNGIEMITIPTDSMSPTINKGDVVFIDTTINYYDNEGVY
                     IFVIDDEVYIKRLQKIPGGIYRALSDNKTYEPFDIKRETLDTAIILGKFIRVLPINPK
                     DL"
BASE COUNT          251 a          116 c          138 g          205 t
ORIGIN      
        1 ccagagattc gcagaaaaaa acttacagaa tggttcaaca caagagatat tccagaaaag
       61 gaaaaaagct acatatcaca attaaaatca ggaaaatcat cttttggtga gcgtgcagca
      121 agaagattag agaatgaata tggaatgcct agcttttatt tggactatga tgaagaaaca
      181 gaaagtgcga ttgtgtctgc tatgtcaatt caagaaacac agtctcatca ataccccatt
      241 catctaattg acttcaaagc taaggcgggc gaaacaggtt ttgtcaatac taactatcct
      301 gaaattattc aatctatcta tttctcttta gacggcttgc ttgaaattgt agggcgaaaa
      361 tcaagcaatg gcattgagat gatcaccatt ccaactgata gtatgagccc aacaataaac
      421 aaaggggatg ttgtttttat tgatactaca atcaattatt acgataacga gggtgtttat
      481 atttttgtta ttgatgatga agtttatata aaaagactac aaaaaattcc aggtggtatt
      541 tatagagccc tatcggacaa caagacttac gaaccttttg atataaagag agaaacctta
      601 gatactgcta ttatccttgg gaaatttatt cgagtattgc caataaatcc taaagacttg
      661 taggcattag cgtagcggtt gagcatcgga gctatttgta gccagcggtt
//
LOCUS       DANLYI010000333          667 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.335, whole genome shotgun sequence.
ACCESSION   DANLYI010000333 DANLYI010000000
VERSION     DANLYI010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..667
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.335"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            <1..286
                     /locus_tag="PXD81_002525"
     CDS             <1..286
                     /locus_tag="PXD81_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5538500.1"
                     /translation="YNKNGQGAVSERNIIDAKNRFKASHPYAKIISCVRGSETYVTRP
                     AKANATTKSVKEKQSQPQESSSLSSMLLGAAVTAGVGFVANKWFSKKDNT"
     gene            314..>667
                     /locus_tag="PXD81_002526"
     CDS             314..>667
                     /locus_tag="PXD81_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5538501.1"
                     /translation="MAGLLPPYFKKKNSDHYKDAVKQFGKEQVDEVIAQCDELESWVK
                     EMPTKQLIMYVGCFHTFNKLSQSKAYVQENFPNVLENPFLFQTLSAASHNELKINRGY
                     KFKEKMLDEFYNLIEL"
BASE COUNT          247 a          105 c          120 g          195 t
ORIGIN      
        1 atataacaag aatgggcaag gtgctgtttc tgaaagaaat attatagatg ctaaaaatcg
       61 ttttaaagcg agccatccat acgcaaaaat tatttcttgt gttagagggt ctgagacata
      121 tgtaacacgt ccagcaaaag ctaatgctac aacaaaatct gtcaaggaaa agcaatctca
      181 accacaagaa agttcatcgc tcagttcaat gttacttggt gcggctgtaa ctgctggggt
      241 tggttttgta gccaataaat ggtttagcaa aaaagataac acttaattta tctctaacca
      301 atgtggagta aaaatggctg gattattacc accttatttc aaaaagaaaa atagtgatca
      361 ctataaagat gctgtaaaac aatttgggaa agaacaagtt gatgaggtta tagcacagtg
      421 tgatgaactt gaatcttggg taaaagaaat gccaacaaaa caacttatta tgtatgttgg
      481 ttgtttccat acttttaaca aattatctca atctaaagct tatgttcaag agaactttcc
      541 aaacgtactg gaaaacccgt ttcttttcca aacactttcg gcagcgtctc ataatgaact
      601 taaaataaat cgaggatata aattcaaaga aaaaatgctt gatgaattct ataatcttat
      661 agagttg
//
LOCUS       DANLYI010000334          644 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.336, whole genome shotgun sequence.
ACCESSION   DANLYI010000334 DANLYI010000000
VERSION     DANLYI010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..644
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.336"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            22..>644
                     /locus_tag="PXD81_002527"
     CDS             22..>644
                     /locus_tag="PXD81_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL5538502.1"
                     /translation="MGMSRDTSYRYQQAVEQSGVDALLNQNRRVPNLKNRVDEATEQA
                     VVKFAFDNPAFGQVRVSNELRKQGIFISAGGVRSIWLRHNLANFKQRLNALEKAVAEK
                     GIILSENQVQALERKKEDDVACGEIETAHPGYLGSQDTFYVGNLKGVGRIYQQTFIDT
                     YSKVAFAKLYTMKTAISAADMLNDKVLPFFESQGLPMLRILTDRGSE"
BASE COUNT          191 a          118 c          160 g          175 t
ORIGIN      
        1 atctctcaag cctgtaaggt gatggggatg agtcgagata catcctatcg ctatcaacaa
       61 gcagttgagc aaagtggggt ggatgcctta cttaatcaaa atcggcgtgt tcctaatcta
      121 aaaaatcgtg tggatgaggc aacagagcaa gctgttgtga agtttgcatt cgataatccg
      181 gcatttgggc aggtacgagt cagtaacgaa ctccgtaaac aaggtatttt tatttcagca
      241 ggtggtgtgc gttctatttg gctacgccat aatctcgcta actttaaaca gcgtttaaat
      301 gcgttagaaa aagcagtggc tgagaaaggc attattctga gtgaaaacca agttcaagcc
      361 ttggagcgta aaaaagaaga cgatgtggct tgtggcgaaa ttgaaacggc tcatccgggc
      421 tatctaggtt cacaagatac cttctatgtg gggaatctta agggggttgg acgcatttat
      481 caacagacct ttattgatac ttatagtaaa gtcgcatttg caaaactgta tacgatgaag
      541 acagcaatta gtgcagcaga tatgctcaat gataaagtcc taccgttctt tgagtcccaa
      601 ggattaccga tgttgcgtat tcttaccgac cgaggcagtg agta
//
LOCUS       DANLYI010000335          634 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.337, whole genome shotgun sequence.
ACCESSION   DANLYI010000335 DANLYI010000000
VERSION     DANLYI010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..634
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.337"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            complement(116..191)
                     /locus_tag="PXD81_002528"
     tRNA            complement(116..191)
                     /locus_tag="PXD81_002528"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(156..158),aa:Thr,seq:ggt)
     gene            complement(195..269)
                     /locus_tag="PXD81_002529"
     tRNA            complement(195..269)
                     /locus_tag="PXD81_002529"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(234..236),aa:Gly,seq:tcc)
     gene            complement(313..397)
                     /locus_tag="PXD81_002530"
     tRNA            complement(313..397)
                     /locus_tag="PXD81_002530"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(361..363),aa:Tyr,seq:gta)
     gene            complement(432..507)
                     /locus_tag="PXD81_002531"
     tRNA            complement(432..507)
                     /locus_tag="PXD81_002531"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(472..474),aa:Thr,seq:tgt)
BASE COUNT          198 a          130 c          139 g          167 t
ORIGIN      
        1 tttctaaaag cctcttaaac aaaaacggtt acaatggaac aaaagaaccc attaaccata
       61 atgtagaaat taataccaat aaaaatttgc gtgagaaaga tagaattcag agaagtggtg
      121 ctgataggcg gatttgaacc gccgacctca cccttaccaa gggtgcgctc taccaactga
      181 gctatatcag cgcttggagc gggcagcggg aatcgaaccc gcatcattag cttggaaggc
      241 taaggtaata gccattatac gatgcccgct gttctaaatt cgtctgacct acgcgattca
      301 aaatatcaga gatggtggag ggagaaggat tcgaaccttc gaaggctgag ccaacagatt
      361 tacagtctgc cccctttggc cgctcgggaa cccctccacg ataattgaat acttatttac
      421 gcctgagtta atggtgccga ctaccggaat cgaactggtg acctactgat tacaagtcag
      481 ttgctctacc tactgagcta agtcggcgtt gctgtgtcgt aagcaagtga tgtgcattat
      541 agggaaattt taacagcgtg caagtgtttt ttaaaaaaat attaaaaaaa gcgttctttc
      601 gatttttaaa tatacaaaat gactattttt tgta
//
LOCUS       DANLYI010000336          633 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.338, whole genome shotgun sequence.
ACCESSION   DANLYI010000336 DANLYI010000000
VERSION     DANLYI010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..633
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.338"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            <1..494
                     /locus_tag="PXD81_002532"
     CDS             <1..494
                     /locus_tag="PXD81_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="primosomal replication protein"
                     /protein_id="HDL5538503.1"
                     /translation="IKQNLSELNVFSTDNIFLNSPYFSQQTGLVSVFIAEIEKTVELL
                     LNQTEVLYSEFYAEKLVKQVDALKNAVEKIQSKPESAQFHSSYQFSPNIHRLAPNKRL
                     QEYRKALRALNEKISWLVEQNLNTQNEATKQTLQNQITETEYRKMKCLKAIEDLEQEL
                     LFK"
     gene            complement(498..571)
                     /locus_tag="PXD81_002533"
     tRNA            complement(498..571)
                     /locus_tag="PXD81_002533"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(537..539),aa:Cys,seq:gca)
BASE COUNT          230 a          117 c          121 g          165 t
ORIGIN      
        1 aaataaaaca aaacctctcg gaattaaatg tattttcaac ggataatatt tttctgaatt
       61 caccctattt ctcacagcaa accggtttgg tgagtgtgtt cattgctgaa attgaaaaaa
      121 cagttgagct attattgaac caaactgagg tgttatatag tgaattttac gctgaaaaat
      181 tggttaaaca agttgatgca ttaaagaatg ccgtagaaaa aatccaaagt aagccagaat
      241 cagctcaatt ccattcttcc tatcaatttt ctccaaatat tcatcgttta gcgcccaaca
      301 aacgcttaca agaataccgt aaagcgttac gtgcattgaa cgagaaaatt agctggttag
      361 ttgagcaaaa tttaaatacg caaaatgaag caactaaaca aaccctgcaa aatcaaatta
      421 cagagacaga atatcgcaaa atgaaatgtt taaaagcaat tgaagattta gaacaagagt
      481 tgctatttaa atagtgatgg aggcgtgtcc cggagtcgaa ccgagctaca tggatttgca
      541 atccagtgca taaccgcttt gctaacacgc cataagattt ggaaattgga gcgggaaacg
      601 aggctcgaac tcgcgacccc gaccttggca agg
//
LOCUS       DANLYI010000337          625 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.339, whole genome shotgun sequence.
ACCESSION   DANLYI010000337 DANLYI010000000
VERSION     DANLYI010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..625
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.339"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            <1..609
                     /locus_tag="PXD81_002534"
     CDS             <1..609
                     /locus_tag="PXD81_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015586989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin-binding protein-like solute binding
                     protein"
                     /protein_id="HDL5538504.1"
                     /translation="AEEAKKIAEAKGIGNKEYKDGLNELEEKNETGKNGEEIRTHGYL
                     YNSHYSVVTAKMKQTLQENGRQMEPTVEVKGLKTENLPTEGKATYKGEAFDSHGNNSN
                     SVVGGELIYNVDFSSRTGSGLVKNVQGGSIELAQGEIKNDSIIASAHQKYNDQAVGNG
                     SYNIQFFGPNAEEIGGKIELNGEGEGSMKQMLGIAGTREEQK"
BASE COUNT          246 a           89 c          143 g          147 t
ORIGIN      
        1 gctgaagaag caaaaaaaat tgcagaggca aaaggaattg gcaataaaga atataaagat
       61 ggattaaatg agttagaaga aaaaaatgaa acaggtaaaa acggagaaga gattcgaacc
      121 catggttatc tctataacag ccattattcc gttgtaactg ccaaaatgaa acaaacttta
      181 caggaaaatg gtcgtcaaat ggaacctacg gttgaagtga aagggcttaa aactgagaat
      241 ttaccgacag agggcaaagc aacatataaa ggagaagcct ttgattctca cggtaataac
      301 agcaattcag tagtaggtgg agaattaatc tataatgttg atttctctag tcgtaccgga
      361 tcaggtctag ttaaaaatgt acagggcggt tctatcgagt tagcacaagg cgaaataaaa
      421 aatgatagta ttatagcttc tgctcaccaa aagtataatg atcaggctgt tggaaatggt
      481 agttacaata tccaattctt tggtccaaat gcagaagaaa ttggagggaa aattgagctt
      541 aatggtgagg gcgaaggctc aatgaagcag atgttaggta ttgcaggaac ccgagaagaa
      601 caaaaataac atttaatacc caatg
//
LOCUS       DANLYI010000338          597 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.340, whole genome shotgun sequence.
ACCESSION   DANLYI010000338 DANLYI010000000
VERSION     DANLYI010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..597
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.340"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            422..>597
                     /locus_tag="PXD81_002535"
     CDS             422..>597
                     /locus_tag="PXD81_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012340801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5538505.1"
                     /translation="MKEANMTNKFTGYKKLSINDLKKFEYNLEHIRNAAIFFKDHFLS
                     KEMIYSTEKESTSV"
BASE COUNT          214 a           98 c          101 g          184 t
ORIGIN      
        1 tgcgtagaac tgttcaaata atattcgcaa atacacactt actacgatgt atccagccaa
       61 tacatccact aggattttct gtccgaattc cagacgatcc tgatcttaca cattctttca
      121 agtttgtaaa gcctgataat gattttatag tatatttttt acaggttaga ttacaaatat
      181 aatatatact agcttgactt taggagataa tcattataat ctgattgtca aggtggcgtt
      241 accttaataa cgcaatgagt tgttggctca tcgtcaatct gatattaatc taggatgtaa
      301 aggcctagcg gtggcaggca ccagaaaact ctgatactaa tctaggatgt aaaggcctag
      361 cggtggcaga caccagaata aaccaacatt taggcccact taattgtggg cttaatactt
      421 tatgaaagaa gcaaatatga ctaataaatt tactggatac aaaaaactaa gcattaatga
      481 cttgaaaaaa tttgagtata atttagagca tataagaaat gccgcaatct ttttcaaaga
      541 tcattttctt agtaaagaaa tgatatatag tactgaaaaa gaaagtacat ccgttat
//
LOCUS       DANLYI010000339          583 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.341, whole genome shotgun sequence.
ACCESSION   DANLYI010000339 DANLYI010000000
VERSION     DANLYI010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..583
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.341"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            <1..206
                     /locus_tag="PXD81_002536"
     CDS             <1..206
                     /locus_tag="PXD81_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Type II secretory pathway, pseudopilin PulG"
                     /protein_id="HDL5538506.1"
                     /translation="YTPTHQQIVLKNKSLYPKSFINVDGVSGQLESKCIKLSLNKENE
                     ILQFEQLGRVYVAAKNKRSTCKD"
     gene            238..>583
                     /locus_tag="PXD81_002537"
     CDS             238..>583
                     /locus_tag="PXD81_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5538507.1"
                     /translation="MASALGAFLLLLVASSYADFYYTQTKQKEQLYLQAEAHQLLNYF
                     QQHFQHIGYQGSHRTDSNFDLFQQAGKSLNIVHKNCIIGFYDLNQDGCLGKRKTKTAA
                     CKLGTVNNTREVL"
BASE COUNT          208 a           92 c          109 g          174 t
ORIGIN      
        1 tttatacgcc gactcatcag cagattgtat taaaaaataa aagtttatat ccgaaatcct
       61 ttattaatgt tgatggggta agtggtcaat tagaatcaaa atgtattaag ctcagtttaa
      121 ataaagaaaa tgaaattttg caatttgaac aattaggacg ggtttatgtg gcagcaaaaa
      181 ataaacgtag cacctgcaaa gattagttta tctgctttta gcctaattga agtattaatg
      241 gctagtgcat taggggcgtt tttattattg cttgttgctt caagttatgc cgatttctat
      301 tacacacaaa ccaaacaaaa agaacagctc tacttgcaag cagaagctca ccagttacta
      361 aactacttcc aacagcattt tcaacacatt ggctatcaag gctctcacag aacagacagt
      421 aattttgatt tatttcagca agcagggaaa agcctgaata ttgtgcataa aaattgcatt
      481 attggatttt acgatttaaa ccaagatggc tgtttgggaa aacgtaagac aaaaacggcc
      541 gcttgtaagt taggaacagt gaataatacc agagaggtgt taa
//
LOCUS       DANLYI010000340          582 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.342, whole genome shotgun sequence.
ACCESSION   DANLYI010000340 DANLYI010000000
VERSION     DANLYI010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..582
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.342"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            <1..>582
                     /locus_tag="PXD81_002538"
     CDS             <1..>582
                     /locus_tag="PXD81_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HDL5538508.1"
                     /translation="GVNQSNIKALQFFIYQSQQTEEPLQRRFENSEIYGPFVVKSENH
                     TYNQYFIKAVDAQKEWINTILDSPFIMAVLLMFAGIPLLIWLSFKITKPVKELTISAN
                     AVASGNLETNPKLEAEWIYEIGEVGKSFNHMVISLKNLTQQQQRMISDISHELKTPLA
                     RLQLAIAILRRKTCGLAEIDRIEAEVGKLDQLI"
BASE COUNT          209 a           93 c          105 g          175 t
ORIGIN      
        1 tggcgtaaac caaagtaata ttaaagcatt gcagtttttt atttatcaat cacaacagac
       61 tgaagagccg ttacaaagac ggtttgaaaa tagtgaaatc tatggtccat ttgtcgtgaa
      121 atccgaaaac catacttata accaatattt tattaaagcg gtagatgctc aaaaagagtg
      181 gattaatacg attttagact caccttttat tatggcagta ttacttatgt ttgccggaat
      241 tcctttatta atatggttat cctttaaaat taccaaaccg gttaaagaat taactatctc
      301 ggcaaatgcg gtagcctctg gtaatttgga aactaatcct aaattagaag cagaatggat
      361 ctatgaaatt ggggaagttg gaaaaagctt taatcatatg gtgataagct taaaaaactt
      421 aactcagcag caacaaagaa tgatttcgga tatatctcat gagttaaaaa cacctttagc
      481 cagattacaa ctcgctattg ctatattacg ccgtaaaacc tgtggtctgg cagaaatcga
      541 tcgcattgaa gcagaagtag gtaaattaga ccaattgatt aa
//
LOCUS       DANLYI010000341          579 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.343, whole genome shotgun sequence.
ACCESSION   DANLYI010000341 DANLYI010000000
VERSION     DANLYI010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 579)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..579
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.343"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
BASE COUNT          191 a          116 c           83 g          189 t
ORIGIN      
        1 tactttaatt gcatcttcag aaagtaaatc acaacgaggt aatacttcat tgatagcctt
       61 ccaagaaagt aaatcttgct actctatagc acctttcatt aaaagtggtt gtttctcaga
      121 ataactttca cgaaattctt gataagaaat tggaaattga attttcattt tcatctccac
      181 tagccttgat aatctgtacc atcaccacaa tcgtagtttg agccagattg gttattattc
      241 gttctacctg gaactccaaa cgtaggcttt attggtaaag atgatggaat taattctttt
      301 acaccgctag gtaaagaatt ttcatgtatt gtaataggta ggctcctcgc taaatcaagg
      361 gctaaattaa cagaagtact ggataaaccg cctaaagccc ccctcttgca aaccctgtag
      421 caaaagcaac gccataagat acaagatctg gttgattagc cttattttca gaattccctc
      481 caactactaa gcttaattgt aaaattgatg gttctttcat ttcattctcc ctttaatcta
      541 attctgatca caaaaaccaa tattaaaaat aattctaaa
//
LOCUS       DANLYI010000342          577 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.344, whole genome shotgun sequence.
ACCESSION   DANLYI010000342 DANLYI010000000
VERSION     DANLYI010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..577
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.344"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            complement(<1..>577)
                     /locus_tag="PXD81_002539"
     CDS             complement(<1..>577)
                     /locus_tag="PXD81_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5538509.1"
                     /translation="NFFPEFITILGTGAFNGFLDFPSLVGKVFPEIIDTINEGLDLDI
                     AIENFEVLSQEEMTECMGLYEQALIKKAEEGEIGLLNVDEINSFYEDNSELILLAKQP
                     MVEEIIPKPDSKASSAILKFFDSNSNILIAKLKESGLQLSAVALQNDQNIARVANVVY
                     NLLPGMVRIFVSYDTVENFLLNNRQWLINKLV"
BASE COUNT          190 a          113 c           79 g          195 t
ORIGIN      
        1 aactaattta ttaatcaacc attggcggtt atttaataag aaattttcta cggtatcgta
       61 acttacgaaa attcttacca tgcccggcaa taagttataa acgacatttg ctactcgtgc
      121 gatattttgg tcgttttgta atgccacagc acttaattgc aaaccacttt cttttaattt
      181 agcaattaag atatttgagt ttgaatcaaa gaattttaga attgcagagg aggctttgct
      241 atctggttta gggattatct cttcaaccat aggttgttta gcaagtaaga ttagctcact
      301 attatcttca taaaagctat ttatttcatc aacatttaat aaaccaattt cgccttcttc
      361 agctttctta atgagagcct gctcatataa acccatacat tcagtcattt cttcctgact
      421 caacacttca aagttctcaa tcgcaatatc taaatctaaa ccctcattta tcgtatcaat
      481 aatttctgga aaaacctttc ctaccaaact agggaagtct aaaaatccat taaacgcacc
      541 agtcccgaga atagtgataa attcgggaaa aaagtta
//
LOCUS       DANLYI010000343          573 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.345, whole genome shotgun sequence.
ACCESSION   DANLYI010000343 DANLYI010000000
VERSION     DANLYI010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..573
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.345"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            <1..>573
                     /locus_tag="PXD81_002540"
     CDS             <1..>573
                     /locus_tag="PXD81_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDL5538510.1"
                     /translation="PTMMSRRVYELLACGTPVISTPSLAIDEQFKGIVQVAKDAKEAN
                     KIAKRLLENEWEWLRISHLGYREVMLKHTYEHRAVQIANTLGQDIKPEAPLASIVVAS
                     NRPYFIDRIVDNVTNQSYPNIEVIVIAQKYTAEQLDNLKQKLSKSNKLKNVIVVQNDS
                     DDTLGKRLNQGIKLSKGEYVAKFDDDDFYFP"
BASE COUNT          214 a           83 c          110 g          166 t
ORIGIN      
        1 tcctacaatg atgtcacgca gagtgtatga attattggca tgcggtacac ctgtaatttc
       61 tacaccatca ttagcaatag atgaacaatt taaaggtatt gtacaagtag ctaaagatgc
      121 caaagaagcc aataaaattg ctaagagatt gttagaaaat gaatgggagt ggctaagaat
      181 ttctcattta ggctatcgag aggtaatgtt aaaacatact tatgagcata gagctgtaca
      241 aatagcaaat acattagggc aggatattaa gcctgaagcc ccattggcaa gtattgttgt
      301 tgcatctaat cgaccatatt ttatagatag aattgttgat aatgttacaa atcaaagcta
      361 tccaaatatt gaagtgatcg tcattgctca aaaatacact gctgaacaat tagataactt
      421 aaaacagaag ctctccaaga gtaataaact gaaaaatgtg attgttgttc aaaatgacag
      481 tgatgatact ttgggtaaac gattgaacca aggaattaaa ttatccaaag gggaatatgt
      541 agcaaaattt gatgatgatg acttttattt ccc
//
LOCUS       DANLYI010000344          572 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.346, whole genome shotgun sequence.
ACCESSION   DANLYI010000344 DANLYI010000000
VERSION     DANLYI010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..572
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.346"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            <1..>572
                     /locus_tag="PXD81_002541"
     CDS             <1..>572
                     /locus_tag="PXD81_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018650961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HDL5538511.1"
                     /translation="QYDLVLLDVMMPVLSGVKTLKRIRQKYSLPVLMLSARDDQIDRI
                     LGLELGADDYVPKPFNNRELVARMKAILRRTTSRPASTDHSEQNYTENISKSLQFAGV
                     ELHSGRQQAYYQGCDLDLTGTEFALLQMLMRNSGEILSRELLSLKILGKNLTPYDRAI
                     DMHISNLRKKLPEREDGLPWFKTLRGRGYL"
BASE COUNT          196 a           89 c          113 g          174 t
ORIGIN      
        1 atcaatatga tttagtatta ctagatgtga tgatgccggt attaagtggc gtgaaaacat
       61 taaaacgtat acgacaaaaa tactcattac ccgttttaat gttaagtgca agagatgatc
      121 aaattgatag aatcttaggt ttagaattag gggctgacga ttatgtaccg aaacctttta
      181 ataatcgtga actagtcgct agaatgaaag cgatacttcg aagaacaacc tctcgcccag
      241 cttcaacaga ccattctgaa cagaattata ctgaaaacat ctctaaatca ttacaatttg
      301 ccggagttga attacattca ggacgtcagc aggcttatta tcaaggatgt gatcttgatt
      361 taacggggac tgaatttgca ttattacaaa tgttaatgcg aaactcaggg gagattttgt
      421 ccagagaatt attgagttta aaaattttag ggaaaaattt aacgccttat gatcgtgcaa
      481 ttgatatgca tatttctaat cttcgtaaaa aattacccga acgggaagat ggattacctt
      541 ggtttaaaac cttaagaggt cggggttact ta
//
LOCUS       DANLYI010000345          569 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.347, whole genome shotgun sequence.
ACCESSION   DANLYI010000345 DANLYI010000000
VERSION     DANLYI010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..569
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.347"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            124..549
                     /locus_tag="PXD81_002542"
     CDS             124..549
                     /locus_tag="PXD81_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HDL5538512.1"
                     /translation="MYKHILVAVDLSEESLVLVRKGAALAEKCGAKLSLIHVDVNFSD
                     LYTGLIDINMSSVQYSVVEETNTALAELASKVNYPVSECLNGTGDFSQVLEEAVEKHN
                     VDLLITGHHQDFWSKFMSSTRRVMNNITVDMLVVPLADE"
BASE COUNT          190 a           84 c          114 g          181 t
ORIGIN      
        1 aaagaggtat gaaaaatata atcaaatttt tatttttgag atctacttca agtttttatc
       61 aaaaagagtg tgttagtatg tttatacaca aagtaaacaa ctctcacaag caaggagcat
      121 attatgtata aacatatctt agttgcagtg gatctttctg aagaaagtct tgttttagtg
      181 cgtaaggggg ctgcgttagc agaaaaatgc ggagctaaac tttccctcat tcacgttgat
      241 gttaattttt cggatctcta tacaggatta attgatatta atatgtcttc ggtacaatac
      301 agtgttgttg aagaaacaaa tacggcactg gctgaacttg cctcaaaagt aaattatcct
      361 gtatctgaat gtttaaacgg aacgggagat tttagtcaag ttttggaaga ggccgtagaa
      421 aaacacaatg tcgatttact catcactggc catcatcagg acttctggag taaatttatg
      481 tcttcaactc gtcgagtcat gaataatatc actgttgata tgctggttgt gccattagca
      541 gatgaatagc aagaaagtga atgaaaaat
//
LOCUS       DANLYI010000346          569 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.348, whole genome shotgun sequence.
ACCESSION   DANLYI010000346 DANLYI010000000
VERSION     DANLYI010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..569
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.348"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            80..517
                     /locus_tag="PXD81_002543"
     CDS             80..517
                     /locus_tag="PXD81_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF412 domain-containing protein"
                     /protein_id="HDL5538513.1"
                     /translation="MLSLFDAGQRYLNTLPNQKKLANFMPDYHIIRLVKFSSKVMPAF
                     ACFALVWQYCFTDPTQSILANSVLTAVFAISIPFQGLYWLGKRARSPLSLSLLEWYES
                     LRQKLISAQVKLEETQAVPSFQDFANLLKLAEETWGDEYFDEL"
BASE COUNT          184 a          101 c           98 g          186 t
ORIGIN      
        1 ttgagccaaa ctgcattaac ctattgtaga ttttaatcag ttagctaaaa taaaagaaaa
       61 atttgaaaga gataatccta tgttaagctt atttgatgca ggacagcgtt acctcaacac
      121 tctgccgaat cagaaaaaat tagccaattt tatgccagat taccacatta ttcgcttggt
      181 aaaattttcc tctaaagtaa tgcctgcttt tgcttgcttt gcattagttt ggcaatattg
      241 ctttaccgat ccgacccaat ctattttagc caattcggta cttactgctg ttttcgccat
      301 ttctattccg tttcaagggt tatattggct tggaaaacgg gcaagatcac cgttatcttt
      361 atcattattg gaatggtatg agtcgcttag acaaaaatta atttcggcac aagtcaaatt
      421 agaagaaaca caagcggtac cttctttcca agattttgca aatttactca agctagcaga
      481 agagacttgg ggagatgaat attttgatga gttataaaat aaaaaaagcc ccacttaaaa
      541 taagtggggt aaaacttttg ttctaattc
//
LOCUS       DANLYI010000347          566 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.349, whole genome shotgun sequence.
ACCESSION   DANLYI010000347 DANLYI010000000
VERSION     DANLYI010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..566
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.349"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            20..331
                     /locus_tag="PXD81_002544"
     CDS             20..331
                     /locus_tag="PXD81_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HDL5538514.1"
                     /translation="MAKSLDAKREELTNEIRDILKNAEELFNETSDSSTEEYKKLKDK
                     LAKQFDDVKDRFATLKDDTVDSARTVVKQTDTLVQDNPYKAITVAGVVGLLLGVLISR
                     R"
     gene            411..>566
                     /locus_tag="PXD81_002545"
     CDS             411..>566
                     /locus_tag="PXD81_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HDL5538515.1"
                     /translation="MLDNIKSGFRNSLITLLEIAQVRGEMARIEINEQKNQLISVLIF
                     ILLAFVFL"
BASE COUNT          209 a           62 c          108 g          187 t
ORIGIN      
        1 ttaaataagg agagcaatta tggctaaaag cttagatgca aaacgtgaag agttaacaaa
       61 cgagattcgc gatattttga aaaatgcaga ggaattattt aatgaaacat ctgattcttc
      121 tacggaggaa tataaaaagt taaaagataa attagcaaaa cagtttgatg atgtaaaaga
      181 tcgttttgca acattaaaag atgataccgt tgatagtgca agaactgtag ttaaacaaac
      241 tgatacctta gttcaagata atccgtataa agcaattacc gttgccggtg tggtagggtt
      301 attattaggt gttttaattt ctagaagata attatctgta attcgtcatt aagtcattaa
      361 gccacgccat atttggggtg gcttattatt taatataagg aatggttata atgttagata
      421 atattaaatc cggatttaga aatagtctca ttacattatt ggaaatagct caagtgcgtg
      481 gtgagatggc aagaattgaa attaatgagc aaaaaaatca attaatttca gtacttatat
      541 ttatcttatt ggcttttgta ttttta
//
LOCUS       DANLYI010000348          559 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.350, whole genome shotgun sequence.
ACCESSION   DANLYI010000348 DANLYI010000000
VERSION     DANLYI010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..559
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.350"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            <1..>559
                     /locus_tag="PXD81_002546"
     CDS             <1..>559
                     /locus_tag="PXD81_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="adenine methyltransferase"
                     /protein_id="HDL5538516.1"
                     /translation="YSSSSYNESQLRTDFLDPFFGLLGWDITNSKNKLTSEREVIVEE
                     PLKEKVSENVKKPDYTFRFNSQRKFFVEAKKPSVKIKTDPLPAHQVRRYGFTAKLSIS
                     VLSNFEYLAIYDCSEIVDVQQDALYARVRLYHYTEYVKFFDEIMNLLGRESVYSGHFD
                     NEWREISQKIENSKQTVDELFFEQIN"
BASE COUNT          207 a           80 c           90 g          182 t
ORIGIN      
        1 ttattcttca tcctcataca atgagtctca gttaagaact gactttttag accctttctt
       61 tggtttactt ggatgggata taactaactc aaagaataaa ctcacatctg aaagagaagt
      121 gattgttgaa gaaccactaa aagaaaaagt ttcagaaaat gtaaaaaaac cagattatac
      181 atttagattc aattctcaaa gaaagttttt tgttgaagct aaaaagccaa gtgtcaaaat
      241 caagactgac cctttacctg ctcatcaggt aagacgatat ggcttcacag caaaattaag
      301 tatttctgtt ttatctaatt ttgaatatct tgctatatat gattgttcag aaatagttga
      361 tgtacaacaa gatgctctat acgcaagggt acgattatat cactatacag aatatgtgaa
      421 attttttgat gaaattatga atctcttagg tagagaaagt gtttattcag gtcattttga
      481 taatgaatgg cgtgaaatct ctcaaaaaat agaaaactct aagcaaacag tggatgaact
      541 attttttgaa caaattaat
//
LOCUS       DANLYI010000349          557 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.351, whole genome shotgun sequence.
ACCESSION   DANLYI010000349 DANLYI010000000
VERSION     DANLYI010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..557
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.351"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            34..234
                     /locus_tag="PXD81_002547"
     CDS             34..234
                     /locus_tag="PXD81_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDL5538517.1"
                     /translation="MYYKEKPAYLEMCLDSLVSQTLPASEIVVVFDGIIPGDLERIVQ
                     HFTEKLPLVVVSLPQNVGLGRP"
     gene            216..557
                     /locus_tag="PXD81_002548"
                     /pseudo
     CDS             216..557
                     /locus_tag="PXD81_002548"
                     /inference="COORDINATES: protein motif:HMM:NF012745.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
BASE COUNT          178 a           73 c          112 g          194 t
ORIGIN      
        1 aaagtagttg tgaagttttc gatattaata tctttgtatt ataaagaaaa accagcttat
       61 ttagagatgt gtttagattc tttagtatcc caaactttac cagcaagtga aattgttgtg
      121 gtttttgatg gtataattcc tggagattta gagcgaattg tgcaacattt tactgaaaaa
      181 ttaccgcttg ttgttgttag tttaccacaa aatgtgggtt taggccggcc ttaaatgaag
      241 gcttaaaaca ctgctctaat gagtgggtgt ttagaatgga tactgatgat atttgcttac
      301 catctcgatt tgaaaagcaa attgatttta ttagtaaaaa tcctgatgtg gttttattgg
      361 ggggacaagt tgaagaattt gacgaaacaa tgtctaattc tctgggtata aaacaagtgc
      421 ccattaatga tgatgaaatt cgtatttcag cattactaag aaatcctttt aaccatatgg
      481 cagtagccta taaaaaatct gtgatcgagc atgttggagg ctatcagcat catctttata
      541 tggaagatta caattta
//
LOCUS       DANLYI010000350          549 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.352, whole genome shotgun sequence.
ACCESSION   DANLYI010000350 DANLYI010000000
VERSION     DANLYI010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..549
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.352"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            complement(212..373)
                     /locus_tag="PXD81_002549"
     CDS             complement(212..373)
                     /locus_tag="PXD81_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007525341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HDL5538518.1"
                     /translation="MEKIENSSNLVLKLSLENQMLILESLEHPKEPNLVMLEALKKYS
                     LYVAKTSNK"
BASE COUNT          170 a           92 c           92 g          195 t
ORIGIN      
        1 tgtagaaaga gcataaaatg ctctaactgt tctagtctct tatctgtttc cttttttgac
       61 tcaaaacgct tttgcttaaa acgaacttct atcgaatcta caccgatgta aattcgatgt
      121 tcctgcattt tgagtagtcg gacagtttca aaaaaatgtt taggggtgat gtgagaaagg
      181 gttatatttg ccatagttta gcttgaaaac gctatttatt acttgtttta gcaacataca
      241 aagaatactt ttttaaagcc tctagcatta ctaaattagg ctctttagga tgttctaagc
      301 tctcaagaat tagcatttga ttttcaagag aaagctttag aacaagattt gaactattct
      361 ctattttttc cattttaatt atctcattag atgactttcg cataacaaca ggttatgtgt
      421 aaattctcgt gcagtgggac aagcccgatt gtactgcact cgaattgtaa cataacctgt
      481 aggacattat gcgaaaatta ataatataag tttttcatca atcggcggta ttattgaata
      541 cgccatcac
//
LOCUS       DANLYI010000351          546 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.353, whole genome shotgun sequence.
ACCESSION   DANLYI010000351 DANLYI010000000
VERSION     DANLYI010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..546
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.353"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            complement(<1..520)
                     /locus_tag="PXD81_002550"
     CDS             complement(<1..520)
                     /locus_tag="PXD81_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDL5538519.1"
                     /translation="MGRPELERAVLSVQNQTYPCKHYVFVDGEKYHQQAADILKNFPS
                     VIVTYLPMNTGADGWVNSSINAIAPFLVKEDIICYLDDDNWFEPTHVESGVHALLEMQ
                     SDYAYSLRNFYDLDGEFLCIDTIESLGEYNCVYQEPLECNYQLGGVKGVLSINLNDCQ
                     HIDTNCYFIRSEI"
BASE COUNT          201 a          111 c           69 g          165 t
ORIGIN      
        1 ctatctcact acgaataaaa tagcaattcg tatcaatatg ctgacaatca ttcagattta
       61 ttgataaaac acctttaaca ccacctaatt gataattaca ttctaatggt tcttgataaa
      121 cgcaattata ttctcccaac gattcaatcg tatcaataca caaaaactcg ccatctaaat
      181 cataaaaatt acgtaaagag taagcataat cagattgcat ttctaataaa gcatgcacac
      241 cagattcaac gtgagtaggt tcaaaccaat tatcatcatc cagataacaa ataatatctt
      301 ctttcactaa aaatggtgca attgcattaa tgctgctatt tacccaacca tcagccccgg
      361 tattcattgg gagataagta acaataacac ttggaaaatt ctttaaaata tcagccgctt
      421 gttggtgata tttctctcca tcaacaaaaa cataatgttt acaaggataa gtctgatttt
      481 gtacgcttaa aacagctctt tctaattcag gacgaccaat tgttgaactg ataacagcta
      541 cagttg
//
LOCUS       DANLYI010000352          535 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.354, whole genome shotgun sequence.
ACCESSION   DANLYI010000352 DANLYI010000000
VERSION     DANLYI010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..535
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.354"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            20..220
                     /locus_tag="PXD81_002551"
     CDS             20..220
                     /locus_tag="PXD81_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL5538520.1"
                     /translation="MNLTSYLSDRPRGFKADFAKKLGISKSYLRQVETGYSPMPAYLA
                     KKIEEVTNGEVAKSELRPDLWD"
     gene            241..372
                     /locus_tag="PXD81_002552"
                     /pseudo
     CDS             241..372
                     /locus_tag="PXD81_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250081.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
BASE COUNT          164 a           97 c          117 g          157 t
ORIGIN      
        1 ttacttaacg ggagtgttaa tgaacttaac tagttatttg tcagatcgcc ctcgtggttt
       61 caaagctgac tttgcaaaaa agcttggtat ttccaaatct tatttacgac aagttgaaac
      121 agggtattca ccaatgccag cctatcttgc taagaaaatt gaagaagtca cgaatggcga
      181 agtagccaaa tctgagcttc gccccgatct gtgggattaa tttacccaaa ggaatgtgca
      241 atgccacgca atgaattaac aaaaaatgca agagcaatcg gactgagtga attacaattt
      301 agccgtacat ttgcagataa tgtggaaatg gtggcggtga ttattgatta cttaagtatt
      361 gagcttgcgg atattcgttt cgcattacga ttagataata ttcaaggtaa agctaatcca
      421 tttttatttg ctcgtaggct aatgcttgag gaggctagtg ataatgttac agatatcact
      481 cagcctagcc cttggcaaaa tgccggtgta accgctattc acggtggctc aatag
//
LOCUS       DANLYI010000353          534 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.355, whole genome shotgun sequence.
ACCESSION   DANLYI010000353 DANLYI010000000
VERSION     DANLYI010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..534
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.355"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            <1..262
                     /locus_tag="PXD81_002553"
     CDS             <1..262
                     /locus_tag="PXD81_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005608319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5538521.1"
                     /translation="LYGFLRDGATLVANGIVNEPSVDCFSQEIAQFTGCHIFSSLYIA
                     FNTQRSFKSHWDSRDIFAVQMQGKKRWIIHTPTFKKTIVYAS"
     gene            252..>534
                     /locus_tag="PXD81_002554"
     CDS             252..>534
                     /locus_tag="PXD81_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin-like domain-containing protein"
                     /protein_id="HDL5538522.1"
                     /translation="MHRSKDMPEYAPNLDDVYMDIVLEAGDVLYLPRGWWHDPIPVGE
                     ETVHLAVGIFPAYTHNYLTWVSQNMVEKEIARASLSHYESDKELIAQLAE"
BASE COUNT          158 a           81 c          109 g          186 t
ORIGIN      
        1 tttatatggt tttttaagag atggggcaac attagtcgca aatggtattg taaatgaacc
       61 aagtgttgat tgcttctctc aagagatagc tcaatttaca ggctgtcata ttttttcaag
      121 tttatatatt gcgtttaata ctcaaagatc ttttaaaagc cattgggata gtcgtgatat
      181 ttttgcagtt caaatgcagg gtaaaaagcg ttggattatt cacactccaa catttaaaaa
      241 aaccattgtt tatgcatcgt agtaaagata tgcctgaata tgctcctaat cttgatgatg
      301 tatatatgga tattgtatta gaagctggag atgttcttta tttacctcgt ggctggtggc
      361 acgatcctat tcctgttgga gaagaaactg tgcatttagc tgtaggcatt tttcctgctt
      421 atactcataa ctatctgact tgggtatctc aaaatatggt tgagaaagaa attgcccgag
      481 cttctttatc gcattatgag tcagataaag agttgatagc acagttggca gaac
//
LOCUS       DANLYI010000354          529 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.356, whole genome shotgun sequence.
ACCESSION   DANLYI010000354 DANLYI010000000
VERSION     DANLYI010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..529
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.356"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            <1..266
                     /locus_tag="PXD81_002555"
     CDS             <1..266
                     /locus_tag="PXD81_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5538523.1"
                     /translation="FLTGATLVFARDALKKLSFGDLNRGEDSNILEQAKKLGIKMYVT
                     DPFNFVVFRSKELSNHTWQVDDNFFTNKGVFVGEELAKDITKL"
     gene            276..>529
                     /locus_tag="PXD81_002556"
     CDS             276..>529
                     /locus_tag="PXD81_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5538524.1"
                     /translation="MININTIKYSEIFNLRLKRKEKNVPWHIVPKDGTKAFCKQIGAS
                     AVEILREFPTLSDINLNNLPDSFVLKPENGHSDGGVMLLK"
BASE COUNT          202 a           74 c           90 g          163 t
ORIGIN      
        1 atttcttaac aggagcaact ttagtattcg caagagatgc cttaaagaaa ctatcatttg
       61 gagacttaaa tcgtggagaa gattcaaata ttctagaaca agcgaaaaaa ttaggtatca
      121 aaatgtatgt taccgaccca ttcaattttg tcgttttccg ttcaaaagaa ctttctaatc
      181 atacttggca agttgatgat aatttcttca ctaataaagg tgtttttgtt ggcgaagaat
      241 tagctaaaga cattacaaaa ttataggaaa caaagatgat aaatataaat acaattaaat
      301 actctgaaat ttttaattta cgtttaaaaa gaaaagaaaa aaatgtacct tggcatatcg
      361 taccaaaaga tggaacaaag gcattttgta aacaaattgg tgctagtgct gtcgaaattc
      421 tcagggaatt tccaacttta tctgacataa atctgaataa tctgccagat agttttgtat
      481 taaaacctga aaatggccat tcagatggcg gggtaatgct tttaaagaa
//
LOCUS       DANLYI010000355          525 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.357, whole genome shotgun sequence.
ACCESSION   DANLYI010000355 DANLYI010000000
VERSION     DANLYI010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..525
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.357"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            complement(203..>525)
                     /locus_tag="PXD81_002557"
     CDS             complement(203..>525)
                     /locus_tag="PXD81_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="endonuclease domain-containing protein"
                     /protein_id="HDL5538525.1"
                     /translation="RAKQLRTNMTEAEKVLWQALRAKRFLAIKFKRQQVIGNYIVDFV
                     CFSAKLVIELDGSQHLDQQNYDNLRTHFLNGQGFRVLRFWNNQIHSDLEAVLDTIFYE
                     ITRG"
BASE COUNT          177 a          100 c           78 g          170 t
ORIGIN      
        1 gaaaaatcta tccctctccc acaaggggag agggttattc tttcaaaaat tcaaaacaac
       61 taaaaaatta gcattctcct tttgtaggag aaaatgttgt tctttcaaaa ttctaaaata
      121 accgcaaaat taactctcgc cccttgtggg agagagacag ataaatcatt cgttcagaat
      181 gatttatcag agagaggggg aattaccctc tcgtaatttc ataaaaaatt gtatctaaaa
      241 cagcctctag atcgctatga atttgattat tccaaaatct taaaaccctg aacccctgac
      301 cgtttaaaaa atgagttcgt agattatcat agttttgttg atctaaatgt tgtgaaccat
      361 ctaattcaat aactaatttc gctgaaaagc aaacaaaatc gacaatatag ttacctataa
      421 cttgttgccg tttaaatttt atcgctaaaa atcgttttgc tcttaatgct tgccaaagaa
      481 ccttctctgc ttctgtcata ttagtccgaa gctgtttagc tcttt
//
LOCUS       DANLYI010000356          522 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.358, whole genome shotgun sequence.
ACCESSION   DANLYI010000356 DANLYI010000000
VERSION     DANLYI010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..522
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.358"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            <1..>522
                     /locus_tag="PXD81_002558"
     CDS             <1..>522
                     /locus_tag="PXD81_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="HDL5538526.1"
                     /translation="TANKIKRSKDDSIEWHRKNEKEIPFWVITETWDFGALSKFYSLL
                     NGQHQRKIAKKFNVDENTLKNWLNEINIVRNLSAHHSRIWNRKFSDIAIPKLDDPRWS
                     KFKKMQQYFSNLSLDDKAKERIFGRVAILWFLVSQTSTNYHWLDKFEDLLNDFPNLPN
                     TKVELMGFNREILE"
BASE COUNT          191 a           68 c          102 g          161 t
ORIGIN      
        1 acagctaata aaattaagcg tagtaaagat gattctattg aatggcatag aaaaaatgag
       61 aaggaaatcc ctttttgggt aattactgaa acctgggatt ttggtgcatt atctaagttt
      121 tactcattgt taaatgggca acaccaaagg aaaattgcta agaaatttaa tgtagatgag
      181 aatacgttaa agaattggtt gaatgaaata aacattgtaa gaaacctttc tgctcaccat
      241 tctcgtattt ggaacagaaa atttagcgat attgctattc ctaagctaga tgatcctcgt
      301 tggtcgaaat ttaaaaaaat gcagcagtat ttttcaaact tatcgctgga tgacaaagca
      361 aaggagagaa ttttcggaag ggtagctatt ctgtggtttt tagttagtca aaccagcact
      421 aattaccatt ggttagataa gtttgaagat ttattaaatg acttccctaa tttaccgaat
      481 acaaaagtag aactgatggg atttaacaga gaaatattag ag
//
LOCUS       DANLYI010000357          515 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.359, whole genome shotgun sequence.
ACCESSION   DANLYI010000357 DANLYI010000000
VERSION     DANLYI010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..515
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.359"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            complement(<1..>515)
                     /locus_tag="PXD81_002559"
     CDS             complement(<1..>515)
                     /locus_tag="PXD81_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AlwI family type II restriction endonuclease"
                     /protein_id="HDL5538527.1"
                     /translation="IKSPEQAATYVNDERYRRFNTLIDKKFNDSVLIELLNCFEKRDD
                     KRIEELVTDEAAIPTIFEYILGIIWYKVSERQGNILDFMKLSLEANLLPKTHAAGGYA
                     DIIYEYEACTSYPKHSLLLEATLADGNNQRRMEMEPVSRHLGDYRIRFNNPFDYSLFV
                     STYLDKNVISD"
BASE COUNT          157 a          102 c           71 g          185 t
ORIGIN      
        1 aatctgagat tacattttta tctaagtatg tgctaacaaa taggctgtaa tcaaacggat
       61 tattaaatct aatcctataa tcgcctaaat gtcttgaaac aggctccatt tccattcttc
      121 tttgattatt tccatctgca agggttgctt caagcaataa tgaatgtttc ggataagagg
      181 tgcacgcttc atattcatat ataatgtctg cgtaacctcc agctgcgtgg gtcttcggca
      241 ataaattggc ttctaatgat aacttcatga aatccaaaat atttccttgt ctttcgctca
      301 ctttgtacca tataattccc aaaatatatt caaaaatagt aggtattgca gcctcatctg
      361 taacaagttc ttcaatcctt ttatcatcac gtttctcaaa acaatttagt aactctataa
      421 gcacagaatc gttaaatttc ttatcgatca aagtattaaa tcttctgtat cgttcatcat
      481 ttacataagt tgcagcttgt tccggactct taatt
//
LOCUS       DANLYI010000358          509 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.360, whole genome shotgun sequence.
ACCESSION   DANLYI010000358 DANLYI010000000
VERSION     DANLYI010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..509
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.360"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            complement(<1..395)
                     /locus_tag="PXD81_002560"
     CDS             complement(<1..395)
                     /locus_tag="PXD81_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mov34/MPN/PAD-1 family protein"
                     /protein_id="HDL5538528.1"
                     /translation="MELEQQIIDYALQHEPHEMCGFVVFDGNKNRFIPCENQAEDKAN
                     YFEISDLDYIKAEEKGELVAVVHSHPEPNGKPILSVLDRKMQVQTGLDWWLVHNRQIH
                     KFRNVPHLIGREFKHGTMDCYTLYRDAYML"
     gene            complement(399..>509)
                     /locus_tag="PXD81_002561"
     CDS             complement(399..>509)
                     /locus_tag="PXD81_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HDL5538529.1"
                     /translation="ATSDPKADKCSHCLNGCELRGNQRNFGGFVSVNKLG"
BASE COUNT          125 a          115 c          107 g          162 t
ORIGIN      
        1 agcatatagg catcacggta gagcgtgtaa caatccattg tgccgtgttt aaactcacgc
       61 ccgattaaat gtggtacatt gcggaattta tggatttgcc gattatgcac tagccaccaa
      121 tctaaaccgg tctgtacctg cattttgcga tcaagcacag ataatatagg cttgccgttt
      181 ggctcgggat gtgaatgtac caccgctacc agttcgcctt tttcttctgc tttgatgtaa
      241 tcaagatcgg atatttcaaa gtaattggct ttgtcttccg cttggttttc gcaagggata
      301 aatcgatttt tattgccgtc aaaaacaaca aagccgcaca tttcgtgcgg ttcgtgttgg
      361 agggcgtagt cgattatttg ttgttccaat tccattcatt accccaattt attcaccgat
      421 acaaatccac caaaattacg ctgattgcct cgcaattcac aaccgttcag gcaatggctg
      481 catttatcgg cttttgggtc tgatgttgc
//
LOCUS       DANLYI010000359          506 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.361, whole genome shotgun sequence.
ACCESSION   DANLYI010000359 DANLYI010000000
VERSION     DANLYI010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..506
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.361"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            <1..>506
                     /locus_tag="PXD81_002562"
     CDS             <1..>506
                     /locus_tag="PXD81_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015587035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HDL5538530.1"
                     /translation="FTAEWTSERAYLLVGVNGTYETTDWLAFHSVKLEKGNVATDWTP
                     APEDVDSAVSAVSAKIDTIQETLANADSALSSRIDAVTASVGSNLAKITQVSNAVAGV
                     DGKLSATHTIKTETIAGGRKAIAGIALGAAADNRNAESSVIVMADKFEVVKNAQDGQP
                     VRLFGVAQ"
BASE COUNT          139 a          111 c          128 g          128 t
ORIGIN      
        1 cctttactgc cgaatggaca agtgagcgag cctatctgct tgttggtgta aatggtactt
       61 atgagaccac agattggtta gcgtttcaca gcgtcaaact tgaaaaaggc aacgtagcca
      121 cagactggac gccagcacct gaagatgtgg acagtgcggt tagtgcagtt tcggctaaaa
      181 ttgatactat tcaggagact ttagcgaatg ccgactctgc attaagctct cgtattgatg
      241 ccgttactgc ttctgttggc tcaaacttgg caaaaattac tcaagtgagt aatgctgtag
      301 ccggtgtgga cggcaaatta tctgctaccc ataccatcaa aaccgaaacc attgccggtg
      361 gacgtaaagc gattgccggt atcgcacttg gtgccgcggc agacaatcgc aatgcagaat
      421 cctctgtcat tgtaatggca gacaaatttg aggtagtaaa aaatgctcaa gacggccaac
      481 cggtacgatt gtttggtgtg gctcaa
//
LOCUS       DANLYI010000360          502 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.362, whole genome shotgun sequence.
ACCESSION   DANLYI010000360 DANLYI010000000
VERSION     DANLYI010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..502
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.362"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            complement(<1..451)
                     /locus_tag="PXD81_002563"
     CDS             complement(<1..451)
                     /locus_tag="PXD81_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5538531.1"
                     /translation="MSALINLFYLYDPWFFHFLRMSFVVGLVACGYLVFQIIKGKSNG
                     IIIPKDSLIAIIALIGFSIIPLLINSTKEIGVLAMYVKMLILFVFGIAIYNVFYHNQQ
                     GKTLFVRDLKIGIAIQWICGVTALLGLPFMVDFLLSSNAMMPRFFGSE"
BASE COUNT          196 a           98 c           74 g          134 t
ORIGIN      
        1 gttccgaacc aaaaaaacgt ggcatcattg cattgctgga taataaaaaa tccaccataa
       61 acggcaaacc taataaggct gtcacaccac aaatccattg aatagcaatg ccgattttta
      121 aatcacgaac gaataaagtt ttaccttgtt ggttatgata aaaaacatta taaatcgcaa
      181 tcccgaacac aaacaaaatc agcattttga catacattgc cagcacacca atttcttttg
      241 tactgttaat caataatgga ataatgctaa agccgattaa tgcaataatt gcaatcaggc
      301 tatctttagg aataataatg ccattcgatt tgcccttgat tatttggaaa accaaatagc
      361 cacaagcaac aagcccgact acaaacgaca ttcttaaaaa atggaaaaac cacggatcgt
      421 aaagataaaa taagttaatg agtgctgaca tttcagacct attatgaatt tcgctgagta
      481 aaattacgtt taattgccag ta
//
LOCUS       DANLYI010000361          496 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.363, whole genome shotgun sequence.
ACCESSION   DANLYI010000361 DANLYI010000000
VERSION     DANLYI010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..496
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.363"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            <1..>496
                     /locus_tag="PXD81_002564"
     CDS             <1..>496
                     /locus_tag="PXD81_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HDL5538532.1"
                     /translation="VSIINTLNEPFSMLSSVALIKTSQEIDNEYLNYVMNSHYFYNIS
                     IGSMSGTGIPRITLTKLESYLVPVPPLLEQQRIVQKIEELLPLVERYEQTEQQLTKLN
                     NTFPEQLKKSVLHAAIQGKLTEQDPNDELASCLIERIKAEKNRLIAEKKLKKPKSVSE
                     IVMR"
BASE COUNT          183 a           91 c           88 g          134 t
ORIGIN      
        1 gagtaagtat tataaatact cttaatgagc catttagtat gttatccagt gttgccttaa
       61 ttaagacctc acaagaaata gataatgagt acttaaatta tgtaatgaat agtcattatt
      121 tttataacat ttctattgga agtatgtctg gaacaggtat tccaagaata acattaacta
      181 aattagaatc ttatttagtc ccagttccgc cactcctaga acaacagcgc attgtgcaaa
      241 aaatcgagga attattaccg cttgttgaac gctacgagca aaccgaacag caactcacta
      301 aactcaacaa tacattcccc gaacagctga aaaaatcggt gttacacgca gctattcagg
      361 gtaaattaac cgagcaagat ccgaatgatg aactggcatc ttgcctgatt gagcggataa
      421 aagcggagaa aaatcgtctt attgccgaga aaaaactcaa aaaaccaaaa agtgtttcag
      481 agattgtaat gcgtga
//
LOCUS       DANLYI010000362          477 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.364, whole genome shotgun sequence.
ACCESSION   DANLYI010000362 DANLYI010000000
VERSION     DANLYI010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..477
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.364"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            <1..>477
                     /locus_tag="PXD81_002565"
     CDS             <1..>477
                     /locus_tag="PXD81_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDL5538533.1"
                     /translation="ILAIIMTLRCTGIRRGQLVKLKIGDVNLSNRTIYISPEINKNHD
                     YHIVPISDSLYLHIERLITELKIRKQTDNCQLFNLNLFSRCSRRRGQPMSQNQLTHIF
                     RHISDLVGFTSSPHRFRHTVATQLMKKPENVYVVQKLLGHKDISVTLGYIEHDVEM"
BASE COUNT          161 a           83 c           87 g          146 t
ORIGIN      
        1 ttattcttgc gattattatg acattgagat gtacaggcat tcggcgagga caattagtga
       61 aactgaaaat tggtgatgta aatctttcca acaggacgat ttatatttcg ccagaaatca
      121 ataaaaatca tgattaccat atcgtaccga tttcagattc actttatctt catatagaaa
      181 ggcttattac agagttaaaa atcagaaaac aaacagataa ttgccaattg ttcaatctca
      241 atttattttc tcgatgctct cgaagaagag gacagccaat gagccaaaat cagcttacgc
      301 atatatttcg gcatatttct gacttggtcg gttttacttc atcaccacat agatttcgac
      361 atacggttgc aactcaatta atgaagaagc ccgaaaatgt ttatgtggtt cagaaacttc
      421 tagggcataa ggatatcagc gtaacactag gttatattga gcacgatgtg gaaatgt
//
LOCUS       DANLYI010000363          475 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.365, whole genome shotgun sequence.
ACCESSION   DANLYI010000363 DANLYI010000000
VERSION     DANLYI010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..475
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.365"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            <1..>475
                     /locus_tag="PXD81_002566"
     CDS             <1..>475
                     /locus_tag="PXD81_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDL5538534.1"
                     /translation="VIGKINAEKDSITQLGNKNIFCNITSKLSPLFHCPILLSEHFQL
                     WLLAESTVGSLINQDNAIFLQLDDTVNLSVLLSGSLLSKQSKMNVDNMLMPRFSHLSD
                     EIFPELDEIHRYKLVNQVTFPAIVRLIDRYLPNNLKSQEQKISWLCQKIDENQPLA"
BASE COUNT          158 a           77 c           82 g          158 t
ORIGIN      
        1 gtgatcggta aaattaatgc agaaaaagac agtattactc aattgggaaa caaaaatata
       61 ttctgtaaca ttacatctaa actctcgccg ctgtttcatt gtcctatttt attaagtgaa
      121 cattttcagc tgtggttgct tgcagaatct acggttggct cattaattaa ccaagataat
      181 gctatttttc tacaattaga tgatactgtg aatctaagtg tattgcttag tggtagttta
      241 ctgtctaaac aatcaaagat gaatgttgat aatatgctta tgcctagatt tagccatctc
      301 agtgatgaaa tattccctga actagatgaa attcatcgct ataaattagt caatcaggtg
      361 acgttcccag ctattgtgag actcattgat cgttatttgc ctaataattt aaaaagtcag
      421 gaacagaaaa tcagctggct ttgtcaaaaa attgatgaaa atcaaccgct tgcat
//
LOCUS       DANLYI010000364          467 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.366, whole genome shotgun sequence.
ACCESSION   DANLYI010000364 DANLYI010000000
VERSION     DANLYI010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..467
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.366"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            <1..>467
                     /locus_tag="PXD81_002567"
     CDS             <1..>467
                     /locus_tag="PXD81_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5538535.1"
                     /translation="KSVKDCSRDREKISHCTLLNACSKALGFSGGFAGFQEDYLSNLT
                     QFMEKHGLLYLVDLTKQKYPVNDMCMTRSLALKKQDISERIFFSGKEIPKRIFTGFNF
                     RYDMHLANGFRIGNSLKTPYSDIGICEDINEALKNPSIKIDCRDGTIRSMADVV"
BASE COUNT          170 a           64 c           86 g          147 t
ORIGIN      
        1 aaatctgtta aagattgttc tagagataga gaaaaaattt ctcactgtac tttgttaaat
       61 gcttgctcaa aagctttagg attttctgga ggctttgcag ggtttcaaga agattattta
      121 tctaatttga cgcaattcat ggaaaaacat gggctcttat atcttgtaga tttaacaaaa
      181 caaaaatatc cagtaaatga tatgtgcatg acaaggagct tagctctgaa aaagcaagat
      241 atatctgaaa gaattttctt tagcggaaag gaaatcccta agagaatatt tactggattt
      301 aatttccgat atgatatgca tttagctaat ggttttcgta tcggaaattc cttaaaaacc
      361 ccatattctg acataggtat atgtgaagac ataaatgaag cattaaaaaa tccatcaata
      421 aaaattgatt gccgtgatgg cacaatcaga agtatggcag atgtagt
//
LOCUS       DANLYI010000365          461 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.367, whole genome shotgun sequence.
ACCESSION   DANLYI010000365 DANLYI010000000
VERSION     DANLYI010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..461
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.367"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            complement(<1..>461)
                     /locus_tag="PXD81_002568"
     CDS             complement(<1..>461)
                     /locus_tag="PXD81_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HDL5538536.1"
                     /translation="FDSFDLYQHYSYGNGEALNLSSVGLSNKIRPLVNKDKAFGKSNG
                     SIESRFISQYKNEGRTSFSNAYDFTKEASGIADPLWALGTATISGELKDIQIFPGGIA
                     RGNIHYSIRDRFTDPFDTFNFTKGEWNPNGTPYIIQDSWIKSVRFSIDKKI"
BASE COUNT          142 a           89 c           69 g          161 t
ORIGIN      
        1 ttatcttctt gtctatcgaa aatcgtaccg attttatcca gctatcttga ataatataag
       61 gtgttccatt tgggttccat tctcctttag taaaattgaa tgtatcaaat gggtcagtga
      121 atctgtccct gatactataa tggatatttc ccctagcaat accaccggga aaaatttgta
      181 tatctttcaa ttctccacta atggtagctg ttcctaacgc ccataacgga tctgcaatac
      241 ccgatgcttc ttttgtaaaa tcataagcat tggagaaaga tgtgcgtcct tcatttttat
      301 attgcgaaat aaatcggctt tctatacttc cattggattt accaaatgct ttatctttat
      361 tcactagagg acgaatttta ttacttaatc caacggatga aagatttaat gcctctccat
      421 taccataaga ataatgctga tataagtcaa aagaatcaaa t
//
LOCUS       DANLYI010000366          459 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.368, whole genome shotgun sequence.
ACCESSION   DANLYI010000366 DANLYI010000000
VERSION     DANLYI010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..459
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.368"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
BASE COUNT          160 a           62 c           88 g          149 t
ORIGIN      
        1 atcatcaaaa aaggattgtt aagtggttat gggctgaatt ttgggataag aatattatct
       61 cgatgacact tagaattgat ggttatagaa atgctaactt atggcattat ttacgttatc
      121 aatcacggat tgaggtattg tataaaatag caaatgaaca cccgaatcta ctaccattat
      181 tagcctatgt taaacctgag tactggaaat gctcggactt atttagttat caaaattggg
      241 taagaagccg tgataaacag ccttttattt tgtatgctaa atgccaagat aagatgttat
      301 gtgaatcaaa ggggcaatgg agatggttaa aaaaacagtc gagaatagtt gtagatcaat
      361 gttttctaca tgttaatcaa tggcagtaat taacagaaat tcaaatgcct ttcaagatcc
      421 cagtatgtgt catatgtttc atacttacta attttagca
//
LOCUS       DANLYI010000367          456 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.369, whole genome shotgun sequence.
ACCESSION   DANLYI010000367 DANLYI010000000
VERSION     DANLYI010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..456
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.369"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            <1..259
                     /locus_tag="PXD81_002569"
     CDS             <1..259
                     /locus_tag="PXD81_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL5538537.1"
                     /translation="TYRSYDALDVSEFHHERINHSELFAEKQNHINGIENFWNQAKRV
                     LRKYNGINRKNFPLFLKECEFRFNFGTPKEQLKTLRKWCEI"
BASE COUNT          160 a           80 c           77 g          139 t
ORIGIN      
        1 tacctatcgt agctatgatg cccttgatgt gagcgaattt catcacgagc gaatcaacca
       61 ttccgaactg tttgcggaga aacaaaacca tatcaatggc attgaaaatt tttggaatca
      121 agcgaagcga gtactccgaa aatataacgg aattaaccga aaaaatttcc ctttattctt
      181 gaaggaatgt gaatttcggt ttaactttgg tacaccaaaa gaacagctca aaacgttgcg
      241 aaaatggtgt gagatttagg gctaatctac tacagcccca aaaattttca taagggaaac
      301 gtttacgtaa aactcctcag accactcatt cttattttat ataaaaaatg tgatagactt
      361 ctcgcagttt cgttttatat atttaaggaa taactaagtg aaattcaata aaaaattaat
      421 tttaacattt gctgcaacct tagttttaag tgcttg
//
LOCUS       DANLYI010000368          455 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.370, whole genome shotgun sequence.
ACCESSION   DANLYI010000368 DANLYI010000000
VERSION     DANLYI010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..455
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.370"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            <1..>455
                     /locus_tag="PXD81_002570"
     CDS             <1..>455
                     /locus_tag="PXD81_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AlwI family type II restriction endonuclease"
                     /protein_id="HDL5538538.1"
                     /translation="WNWNNEMQEKYYDFMKDRAFLTGEAKRKDKDAREKTSGLVDIGL
                     ITEDRLITDAGRELLKVTSSGDFETNNVFNINRDSFIYLKQLLKTSIDVSGSIVRPFI
                     AVVKCLTELEFLSYDEFTYFVPLIRDDESAKQIISDIKLYREGKILPEE"
BASE COUNT          176 a           50 c           97 g          132 t
ORIGIN      
        1 ttggaactgg aacaatgaaa tgcaagaaaa atattatgat tttatgaaag atagagcttt
       61 cctaacagga gaagcaaaaa gaaaagacaa agatgccaga gaaaaaacat caggattggt
      121 tgatattggt ttaattacag aagatagatt gattacggat gcaggaagag aactccttaa
      181 agtaacaagt agtggggatt ttgaaaccaa taatgtgttt aatattaata gagatagctt
      241 tatctatcta aaacagcttc ttaaaacgag tatagatgta agtggtagta tcgttagacc
      301 gtttattgcg gttgtaaagt gcctgacgga acttgagttt ttaagttatg acgagtttac
      361 ttattttgtt ccgctaatta gagatgacga aagtgcaaaa caaatcatat cggatattaa
      421 attatatcga gaaggtaaaa ttttaccgga agaaa
//
LOCUS       DANLYI010000369          449 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.371, whole genome shotgun sequence.
ACCESSION   DANLYI010000369 DANLYI010000000
VERSION     DANLYI010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..449
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.371"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            <1..>449
                     /locus_tag="PXD81_002571"
     CDS             <1..>449
                     /locus_tag="PXD81_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcription antiterminator BglG"
                     /protein_id="HDL5538539.1"
                     /translation="KNYGYSLSIREQYFIFDIFSGMVNRIELERKPLRLAIIALNGRL
                     STQQLKRNIKRYFSDYILKIETKVLYELAIMEDKPYDYYLCNEYGKNMAIPYKPIYFA
                     DSSLNENEYATSLNEVFYHAYQYDEVLPSIKSIEIEAQYRLNEFHLE"
BASE COUNT          154 a           75 c           77 g          143 t
ORIGIN      
        1 aaaaaactac gggtatagcc tatctattcg agaacagtat tttatttttg atattttttc
       61 cggaatggtt aatcgaattg aattagaacg taaaccgctc cgactggcga ttattgccct
      121 taatggcaga ttaagcaccc aacagttaaa acggaatatt aagcggtact tttccgacta
      181 tattttgaaa attgaaacca aagtgctata tgagcttgcc ataatggaag ataaacctta
      241 tgactactat ctctgtaatg aatatggaaa aaatatggct attccctata agcctattta
      301 ttttgctgac agtagtttaa atgaaaatga atatgcaaca tcactcaacg aagtatttta
      361 tcacgcttat caatatgatg aggtgttgcc tagcattaaa tcgattgaaa ttgaagcaca
      421 atatcgccta aatgagtttc atcttgagc
//
LOCUS       DANLYI010000370          445 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.372, whole genome shotgun sequence.
ACCESSION   DANLYI010000370 DANLYI010000000
VERSION     DANLYI010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..445
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.372"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            <1..>445
                     /locus_tag="PXD81_002572"
     CDS             <1..>445
                     /locus_tag="PXD81_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HDL5538540.1"
                     /translation="MPRGDKNAMKGFIFNIPSIEYQKKCIANYFAFDQKIQLNTQTNQ
                     TLEAIAQAIFKSWFVDFDPVRAKAAALSEGKSEHEANLAAMSVICGKDTSELNDTEYK
                     ALWQIAEAFPSEFGDEGLPIGWKFNQADNLFDVGIGKTPPRKESEW"
BASE COUNT          153 a           96 c           96 g          100 t
ORIGIN      
        1 aatgccacga ggggataaaa atgcaatgaa aggttttatt ttcaatatcc caagtattga
       61 atatcaaaaa aaatgtattg ctaattattt tgcctttgac caaaaaatcc aactcaacac
      121 ccaaaccaac caaaccctag aagccattgc acaggcaatc ttcaaaagct ggtttgtgga
      181 ttttgaccct gtgcgcgcca aagccgccgc cctaagcgaa ggcaaaagcg aacacgaagc
      241 caaccttgcg gcaatgtcgg tgatttgtgg taaagacacc agcgagttaa acgacaccga
      301 atacaaagcg ctttggcaaa tcgccgaagc cttcccaagt gagtttggag atgaagggtt
      361 acctattggc tggaaattca atcaagcaga caatttattt gatgttggta ttgggaaaac
      421 cccaccaaga aaagagagtg aatgg
//
LOCUS       DANLYI010000371          432 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.373, whole genome shotgun sequence.
ACCESSION   DANLYI010000371 DANLYI010000000
VERSION     DANLYI010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..432
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.373"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            116..>432
                     /gene="msrA"
                     /locus_tag="PXD81_002573"
     CDS             116..>432
                     /gene="msrA"
                     /locus_tag="PXD81_002573"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HDL5538541.1"
                     /translation="MRSENLQDIYLAGGCFWGIEAYMEKIYGVKEATSGYANGKTEQT
                     AYRIIGQTDHAETVHVTYDANKISLEKLLQYYFKVIDPTSKNKQGNDIGRQYRTGIYY
                     QNE"
BASE COUNT          156 a           78 c           83 g          115 t
ORIGIN      
        1 taaaattaca taagttataa aggagaactg gatgaaatac aatcaactgt tactatgttt
       61 ggtgtctgct atctttacca cctcctcaat agcaaacatc acaaccccta acgagatgcg
      121 atctgaaaac ttacaggata tttacttggc aggaggatgt ttctggggca ttgaagccta
      181 tatggaaaaa atatatggcg taaaagaggc tacatcgggc tatgccaatg gcaaaactga
      241 gcaaactgct taccgaatta tcgggcaaac cgatcatgca gaaacagttc acgtgaccta
      301 tgatgctaat aaaatttctt tagaaaagct gttgcaatat tactttaaag taattgatcc
      361 aaccagtaaa aataaacaag gtaatgatat tggccgccaa tatcggacag gtatttatta
      421 tcagaatgag ca
//
LOCUS       DANLYI010000372          425 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.374, whole genome shotgun sequence.
ACCESSION   DANLYI010000372 DANLYI010000000
VERSION     DANLYI010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..425
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.374"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
BASE COUNT          144 a           80 c           60 g          141 t
ORIGIN      
        1 taaaatttcc caagaactaa tttttagcta cggcgatagc tcgaaatatc tgtttataaa
       61 ataacctaat taataaggca ctttggattt tagttatatc ctgaaaaatc aaattataaa
      121 gaaaccttat ctatcaatac ttgaagccat taagttcaag tctcgaccag gcaccatttg
      181 actaaattta gttcaacaga gctaaacgaa aaaaccttag aaagcctgta aaatcaacgt
      241 ttacaggctt ttttgttgtt ttagctcaat ggcactaaac atttcatatc gcaaatttta
      301 agtagcctag caaattttcc gccaaatatg accgcttgta atgccgttag gttattgcct
      361 ggcggccttt ttgttacagc tcttatattt tttacaccaa agttcagaaa aagtcacttt
      421 tatca
//
LOCUS       DANLYI010000373          422 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.375, whole genome shotgun sequence.
ACCESSION   DANLYI010000373 DANLYI010000000
VERSION     DANLYI010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..422
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.375"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            148..>422
                     /locus_tag="PXD81_002574"
     CDS             148..>422
                     /locus_tag="PXD81_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HDL5538542.1"
                     /translation="MKNRIEHPKIFISYAWGSQEHDEKVIALATNLKGDGIDVIFDKW
                     QLKEGNDTFKFMEKSVLDESITNVLILIDPIYAKKANERAGGVGTET"
BASE COUNT          160 a           45 c           96 g          121 t
ORIGIN      
        1 ttgataaata ccatgaaatg ccattagaga ctgtagattg gcatgatggg atgataaaag
       61 aggctactgg agagtatatg tcatagtgat tataagatag tgagaaagaa tacttatttt
      121 gaaaggaata catgggaatt gatacagatg aagaatagaa ttgaacatcc taagattttt
      181 atttcatatg cttggggatc acaggaacat gatgagaagg ttattgcttt agctacaaat
      241 cttaaaggag atggtataga tgtcattttt gataaatggc agctaaaaga aggcaatgat
      301 acctttaaat tcatggaaaa aagtgtttta gatgagagta ttaccaatgt actgattctt
      361 atagatccta tttatgcaaa gaaagcaaat gaaagagccg gtggcgtagg tacagaaaca
      421 ca
//
LOCUS       DANLYI010000374          418 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.376, whole genome shotgun sequence.
ACCESSION   DANLYI010000374 DANLYI010000000
VERSION     DANLYI010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..418
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.376"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            275..>418
                     /locus_tag="PXD81_002575"
     CDS             275..>418
                     /locus_tag="PXD81_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL5538543.1"
                     /translation="MKVHEKIKLLRENNHWTQDEMAQKLSMTTKGYAKIERGETISNL
                     PKIE"
BASE COUNT          167 a           74 c           54 g          123 t
ORIGIN      
        1 aaaataatta tatccataag agataacata ttctccccaa attgatagtc tttatatgct
       61 gcataaagag gcaaaattac tgatgcagct gacgctaaaa tacctagcat tccatataat
      121 ggattatatt gattattcat gaatgtttct tctcctgtga gcttaagata aaatttactc
      181 tattctaaat tgctcaataa actgctacaa tccacaaaat ttatccttta ccacatcaag
      241 ttatatccca actcccacaa tctataggtg ttatatgaaa gtacacgaaa aaatcaaatt
      301 actgcgagaa aataatcatt ggacacaaga tgaaatggct caaaaattaa gtatgacaac
      361 caaaggttat gcaaaaatag aacgtggtga aactatctct aatttaccga aaatagaa
//
LOCUS       DANLYI010000375          402 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.377, whole genome shotgun sequence.
ACCESSION   DANLYI010000375 DANLYI010000000
VERSION     DANLYI010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..402
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.377"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            <1..>402
                     /locus_tag="PXD81_002576"
     CDS             <1..>402
                     /locus_tag="PXD81_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HDL5538544.1"
                     /translation="LVTAIPNFLEKVNNDFAISARNRDLYSHYLPYQGIDDWENSNFN
                     FRTLNGVYYDDNLTSTELLRKFMIQRDYNTLYALSSARTGNADSLAKNYHLVVAKMSY
                     ENLKKELSKKPDSTFNKLKKLYETNGEFLNEY"
BASE COUNT          146 a           66 c           64 g          126 t
ORIGIN      
        1 ttagtaacag ccattcctaa cttcctggag aaagttaata atgattttgc tataagtgct
       61 agaaatagag acttatatag tcactacctt ccttatcagg gtattgatga ttgggagaat
      121 tctaatttta actttcgaac actaaatgga gtttattatg atgataatct aacttcaaca
      181 gaactcctgc gaaaatttat gattcaaaga gattataaca ctctttatgc tctaagttct
      241 gctcgcacag gaaatgctga tagtctggct aaaaattacc atcttgtagt tgcgaaaatg
      301 tcctatgaaa acctgaaaaa agaattatcc aagaaacccg actctacatt taataaactg
      361 aaaaagctgt atgaaaccaa tggtgaattc ttaaatgaat at
//
LOCUS       DANLYI010000376          396 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.378, whole genome shotgun sequence.
ACCESSION   DANLYI010000376 DANLYI010000000
VERSION     DANLYI010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..396
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.378"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
BASE COUNT          143 a           72 c           67 g          114 t
ORIGIN      
        1 tcaatatatg tacttccaaa gagaactttt gtataaggcg tatcatgata gatcgtgaca
       61 tcagccttca atttgtactc gccttccatg cattccccaa aatggtattg actaaaaaca
      121 atggttatga tataatgaca tggctttgct caataggtaa tcagaccact ttgaaagctc
      181 ggtccttttc aaaagaaaag gtcttactaa ttactcacaa aatctggaga acaatatgaa
      241 aaacactaca gcgcaacaaa agccaatgaa agttacagct aaacatatta gagaatctac
      301 tactcgttgt taagagctta gctttgatat aaaaagacca actttttata tcggcgtagt
      361 aggtctaatt tgagcctacg aaatgaaagt gagtta
//
LOCUS       DANLYI010000377          393 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.379, whole genome shotgun sequence.
ACCESSION   DANLYI010000377 DANLYI010000000
VERSION     DANLYI010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..393
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.379"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            145..393
                     /locus_tag="PXD81_002577"
     CDS             145..393
                     /locus_tag="PXD81_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5538545.1"
                     /translation="MISGEKMTWESLGASAIGGAIGGGIGATMKAGAVTASTLSALGG
                     GMGEGLYKSTSQGIKNSIGIKASCNQSGSDYGDGCDYQ"
BASE COUNT          110 a           44 c          115 g          124 t
ORIGIN      
        1 tagtatttgt tgtaattata aacatcatag aggaatatct cttatgaaag agttaaaaat
       61 tgaggattgc caatttgttt cggcaggctt gcacttgtta ttgtaggagc tgttgctgga
      121 gctgcatatt atgttgggaa tggtatgatt tctggggaaa aaatgacttg ggagagtttg
      181 ggagcatcag ctataggtgg ggctattggt ggcggtattg gagcaacaat gaaagctggt
      241 gcggtgacag cgtcaacctt atctgcttta ggtggtggta tgggggaggg cttgtataaa
      301 agtacttctc aaggtatcaa aaatagtatt ggtataaagg cgagttgcaa tcaatcaggt
      361 tcggattatg gtgatggatg tgattatcaa taa
//
LOCUS       DANLYI010000378          377 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.380, whole genome shotgun sequence.
ACCESSION   DANLYI010000378 DANLYI010000000
VERSION     DANLYI010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..377
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.380"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            10..>377
                     /locus_tag="PXD81_002578"
     CDS             10..>377
                     /locus_tag="PXD81_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase family
                     protein"
                     /protein_id="HDL5538546.1"
                     /translation="MKSLVIKLFGTKKEKKTFKFSKIESVLLKPIGDAIGDAVVHIAH
                     LEQIKKALPNVKIAVLVTSRNKLIFSQANVVDMILEDKVSTYLSQRGHWDLYLDFKPT
                     FTTKSIILDSILKPKFIINFG"
BASE COUNT          137 a           59 c           60 g          121 t
ORIGIN      
        1 attatagaga tgaaatcctt agttataaaa ctttttggta caaaaaaaga gaaaaaaacc
       61 ttcaaattta gcaaaataga atccgtatta ctcaaaccta tcggagatgc tattggcgat
      121 gctgttgtgc atatagctca tttggagcaa attaaaaaag ctctcccaaa tgtaaaaata
      181 gcggttttag ttacatctcg taacaaattg attttctctc aagcaaacgt ggtagatatg
      241 atcttagaag ataaagtatc cacttatctc tcgcagcggg gacattggga tctttattta
      301 gatttcaaac cgacatttac cacaaaaagt attattttag atagtatttt aaagcctaaa
      361 tttattatta acttcgg
//
LOCUS       DANLYI010000379          374 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.381, whole genome shotgun sequence.
ACCESSION   DANLYI010000379 DANLYI010000000
VERSION     DANLYI010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..374
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.381"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
BASE COUNT          119 a           65 c           63 g          127 t
ORIGIN      
        1 agagtttatt attagagagg atatcgccct tctatacttc atacttatgg gattatatcc
       61 tctgtatttc gtaaatataa accgtgaata cgtccattat gatgatcaaa gttattcttt
      121 ataagatcgt aaaatgagct atcattatgt tcataatgcc taaatatggc ataggaaacc
      181 aaatcagcta ataaattaat cttgatgact ttgagtcaag aaacagcgga gcttctgaga
      241 agttcctgag cttcccataa gtatgatcac tatccttaaa tgttcttgca agtgccttaa
      301 ttgacttctc cgttgatgat ttatcaaaga ttgagctcct ttgagtattg ccttgataat
      361 gcaaatgccc aaga
//
LOCUS       DANLYI010000380          373 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.denovo.382, whole genome shotgun sequence.
ACCESSION   DANLYI010000380 DANLYI010000000
VERSION     DANLYI010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..373
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.denovo.382"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            <1..343
                     /locus_tag="PXD81_002579"
     CDS             <1..343
                     /locus_tag="PXD81_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5538547.1"
                     /translation="REEAIEYQSKKWKSTAVLFSRVPALVVFSISFFIFTAFILFIVF
                     GNYTRRETVVGELVMQSHPILLSAPKSGYISENYIQPHQQVKKRAIRWLKLRWIELRI
                     AKILMLILFFR"
BASE COUNT          120 a           68 c           65 g          120 t
ORIGIN      
        1 cagagaagaa gctattgaat atcaatcgaa aaaatggaaa agcacagctg tactatttag
       61 cagagttcca gctttggttg tcttttccat ttctttcttt atctttactg cctttattct
      121 atttattgtg tttggtaatt atacccgccg tgaaacggtt gtgggtgaat tagtgatgca
      181 atctcatccg attttattat ctgccccaaa atccggctat atatccgaaa attatattca
      241 accacatcag caggtaaaaa aaagggcgat ccgctggtta aaattacgct ggatagaatt
      301 acgcatagcg aaaatattaa tgttaattct attctttcgt tgaaatcaca aattcaagca
      361 acagaacaag cga
//
LOCUS       DANLYI010000381         7230 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.guided.124, whole genome shotgun sequence.
ACCESSION   DANLYI010000381 DANLYI010000000
VERSION     DANLYI010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 7230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7230
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.guided.124"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            151..606
                     /locus_tag="PXD81_002580"
     CDS             151..606
                     /locus_tag="PXD81_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005819327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF411 domain-containing protein"
                     /protein_id="HDL5538548.1"
                     /translation="MKLHKFTRSLLLLSLGITAFAQAQTLSMEVWKSPTCGCCNEWIS
                     YMQKNGFEVKVNETGNYDAHKRFNIKYEHASCHTAVIDGYVIEGHVPAEDIKRLLAEK
                     PDAIGLSAPGMPIGSPGMDGEAYGGRKDPYDVVLIKKNGESEVFKSYNQ"
     gene            620..2167
                     /locus_tag="PXD81_002581"
     CDS             620..2167
                     /locus_tag="PXD81_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012340840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase family protein"
                     /protein_id="HDL5538549.1"
                     /translation="MKRRDFLRYGIGISLASSSLYSLANMMNHAQHGNMAMMHSVPTG
                     LMPTEAMPSGLSLNGILPKLANQSTQAGLFKATLKAEPIKIRLADNKETEFWAYNGQL
                     PGPQIEVFEGDTVEIEFINHLPQPSTVHWHGLDVPNEADGNPMDMVEPQGKKVYRFTL
                     PQGSAGTYWYHPHPHDHVSEQVYKGLAGTFVVKAKNDPLAHLPEQHWVISDLRLNADG
                     TIPANTMLDWINGREGEFVLINGQYQPQIQVKTNERIRLWNATSARYFRLNIPGVKWI
                     VVGTEGGLLEKPRSPVDELLLTPAERVEVIMVGETQGTVNLQSGYYDRRKMMVQEQPK
                     DLTLATIQVKSEPVQLPESLRSLPNWDEPKQVRQIRFSEKMMNHTNMPMMNHGTHHAT
                     TTLTDNPIPPMMNGMFLINGQTFDMNRIDFVTKLNEVEQWEIFNESHMDHPFHLHGTQ
                     FEVIQHTLNGKTFKPEGRALKDVVNLRPYEKVIIRFKQGHTGLKMYHCHILEHENLGM
                     MGMFKVE"
     gene            complement(2257..2412)
                     /locus_tag="PXD81_002582"
     CDS             complement(2257..2412)
                     /locus_tag="PXD81_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012340841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HDL5538550.1"
                     /translation="MGLNRAEMMYREGAYVIDPVFPATLGYAGAGVVVAVGDDAGEFQ
                     IGDKVSV"
     gene            2501..2680
                     /locus_tag="PXD81_002583"
     CDS             2501..2680
                     /locus_tag="PXD81_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5538551.1"
                     /translation="MFLVNQLLAITIATSAVLTNAQLAILAVAFWIAILPCARLATAV
                     GVWVFDDVVHNNSPN"
     gene            2729..3247
                     /locus_tag="PXD81_002584"
     CDS             2729..3247
                     /locus_tag="PXD81_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5538552.1"
                     /translation="MPFKTVTFAQATTNDGQSLLAVNDLFIGPKSHTSAQYILQWNGA
                     EEVQSSSGIIVSTGLGSTGWFQSILAGAMAITGEASHPLLQGFSWSDRKLQFSVREPF
                     PSRTTGVALTFGTIEPDSPLQLGSLMPENGVIFSDGIEDDYLQFNAGCIAHIGIADIQ
                     GQLISQKGRQRI"
     gene            complement(3250..3873)
                     /gene="tetR(H)"
                     /locus_tag="PXD81_002585"
     CDS             complement(3250..3873)
                     /gene="tetR(H)"
                     /locus_tag="PXD81_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(H)"
                     /protein_id="HDL5538553.1"
                     /translation="MAKLDKEQVIDNALILLNEVGIEGLTTRKLAQKIGVEQPTLYWH
                     VKNKRALLDALAETILQKHHHHVLPLPNETWQDFLRNNAKSFRQALLMYRDGGKIHAG
                     TRPSESQFETSEQQLQFLCDAGFSLSQAVYALSSIAHFTLGSVLETQEHQESQKEREK
                     VETDTVAYPPLLTQAVAIMDSDNGDAAFLFVLDVMISGLETVLKSAK"
     gene            3965..5167
                     /gene="tet(H)"
                     /locus_tag="PXD81_002586"
     CDS             3965..5167
                     /gene="tet(H)"
                     /locus_tag="PXD81_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(H)"
                     /protein_id="HDL5538554.1"
                     /translation="MNKSIIIILLITVLDAIGIGLIMPVLPTLLNEFVSENSLATHYG
                     VLLALYATMQVIFAPILGRLSDKYGRKPILLFSLLGAALDYLLMAFSTTLWMLYIGRI
                     IAGITGATGAVCASAMSDVTPAKNRTRYFGFLGGAFGVGLIIGPMLGGLLGDISAHMP
                     FIFAAISHSILLILSLLFFRETQKREALVANRTPENQTASNTVTVFFKKSLYFWLATY
                     FIIQLIGQIPATIWVLFTQYRFDWNTTSIGMSLAVLGVLNIFFQAIVAGKLAQKWGEK
                     TTIMISMSIDMMGCLLLAWIGHVWVILPALICLAAGGMGQPALQGYLSKSVDDNAQGK
                     LQGTLVSLTNITGIIGPLLFAFIYSYSVAYWDGLLWLMGAILYAMLLITAYFHQRKTT
                     PKAVISTP"
     gene            complement(5263..6573)
                     /locus_tag="PXD81_002587"
     CDS             complement(5263..6573)
                     /locus_tag="PXD81_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011200814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HDL5538555.1"
                     /translation="MSNILNWPDYKVLQVSELEHDYQVHAEVSEPPTQCPHCNHPEIV
                     GFGRRDEVIMDTPVHGRRTGIMLNRRRYRCQSCRKTFLEPVPHKDEKRQMTNRLIQYI
                     ERESLRRTFSSVAEDVGVDEKTVRNIFNDYCERLEKTLNFEMPQWLGIDEIHIIKPRC
                     VITNIQQQTIVDMLDNRNKTTVTRYLSKRTDRDLVRYVAMDMWRPYRQAVETMIPDAT
                     VIIDKFHVVRMANESLERARKAIRSALTPQQRRGLMRDRFVLLKRRHELTDAEYMRFS
                     GWTLNYPEIGQAYELKEAFFEIWDCQTRHQAQEAYYSWLRQITPEMKAHYDPLIKAMG
                     NWHDDIFAYFDHPITNAYTESLNNLIRVVNRVGRGYSFEALRAKILFTEGFQKIKKPR
                     YQRQRIPEGAMGRMPFYGVAEAGPSTNYGADISTLVREIEAGRL"
     gene            complement(6573..6875)
                     /locus_tag="PXD81_002588"
     CDS             complement(6573..6875)
                     /locus_tag="PXD81_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011200813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5538556.1"
                     /translation="MKIKLYFPDESIATIKRMGLRQMSPETLRWTADHPSSSYGMGAL
                     LRGKSGEILDGKSFAAMVHAFGAWIETDSEDTSRRVHNALVTAATGTEESVKVAKE"
     gene            complement(6916..7104)
                     /locus_tag="PXD81_002589"
     CDS             complement(6916..7104)
                     /locus_tag="PXD81_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011200812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5538557.1"
                     /translation="MSYDANDALNEIEEALSELERVAEDLINNNPNKESELRGQGVHQ
                     ATKHLRFRIRNIRRGEAI"
BASE COUNT         1959 a         1601 c         1599 g         2071 t
ORIGIN      
        1 ggatggtacg ttggcatatc tctactacaa gcagtaggat tttgagaaaa attttcaaat
       61 gatagaattt tttcagatta cttgacctta accctaggtt aagctttaaa atgatgtcca
      121 ttcaatctca ataaaaaata aggacatctt atgaaattac ataaattcac ccgctcattg
      181 ttgcttttaa gtttaggcat aaccgctttt gctcaagcac aaacactttc tatggaagtg
      241 tggaaaagcc cgacttgtgg ctgttgtaat gaatggatca gctatatgca gaaaaacggt
      301 tttgaggtta aagtcaacga aacaggcaat tatgatgctc ataagcgctt taacattaaa
      361 tatgaacatg cttcttgcca cacagccgtg attgatggct atgtgattga aggacatgta
      421 cctgctgagg atatcaaacg tttactcgca gaaaaacccg atgcaatagg cttgtctgca
      481 ccgggaatgc caattggttc accaggtatg gacggtgaag catacggcgg acgcaaagat
      541 ccttatgatg ttgttttgat taagaaaaac ggtgaaagtg aagtgtttaa atcctataac
      601 caataggggg ggggaaagaa tgaaacgtcg agatttttta cgttatggaa ttggaattag
      661 cttagccagt agctcgcttt atagcctcgc gaatatgatg aaccacgcac aacatggcaa
      721 tatggcgatg atgcattcag tacctactgg cctaatgcca actgaggcaa tgccttcagg
      781 tttatcgctt aatgggatat taccaaaact tgctaatcaa tcgactcaag ctggactgtt
      841 caaagccact ttaaaagcag agccaattaa aattcgcctc gcagacaata aagaaacgga
      901 attttgggct tataacggac aactaccagg gccacaaatt gaagtatttg aaggagatac
      961 cgtagaaatt gaatttatca atcacctacc acaaccctca acagtacatt ggcacggctt
     1021 agatgtacca aatgaggcgg atggtaaccc gatggatatg gttgaaccgc aagggaaaaa
     1081 agtctatcgc tttacgctac ctcaaggcag tgctgggaca tattggtacc accctcatcc
     1141 acatgaccat gtttccgaac aagtctataa aggcttagca ggcactttcg ttgttaaagc
     1201 gaaaaatgat ccgcttgcac accttcctga acaacattgg gtgatttccg atctgcgttt
     1261 aaatgccgat ggcaccattc cagcaaacac gatgttagat tggataaacg gtcgtgaggg
     1321 tgaatttgtg ttaatcaacg gtcaatatca gccccagatt caagtgaaaa cgaatgaacg
     1381 aatccgtctt tggaatgcca ctagcgctcg ttattttcgt ttaaacattc caggggtgaa
     1441 atggattgtg gtgggtaccg aaggtggttt acttgaaaaa cctcgctcgc ctgttgatga
     1501 actgctactt acaccagccg aacgcgttga agtgattatg gtgggtgaaa cgcaaggaac
     1561 agtcaattta caaagcggtt attatgatcg ccgtaagatg atggttcaag aacagcctaa
     1621 agatctcact ttagccacaa ttcaggtgaa atcagaacct gttcagttac ctgaaagctt
     1681 aaggtccttg cctaactggg atgagccaaa gcaggttcgc caaattcgct ttagtgaaaa
     1741 aatgatgaac catacgaata tgccaatgat gaatcacggt acacatcacg ctactacaac
     1801 actaacggac aaccctattc caccaatgat gaatggtatg ttcttaatca acggtcaaac
     1861 ctttgatatg aaccgtattg attttgttac caaattgaat gaagtggaac agtgggaaat
     1921 cttcaatgaa agccatatgg atcatccatt ccatttacac ggcactcaat ttgaagtaat
     1981 tcaacatacg ttaaacggta aaacctttaa gccagaaggc agagcgttaa aagatgtggt
     2041 taatttacgc ccttatgaaa aagtgattat tcgctttaag caaggacata ctgggttgaa
     2101 aatgtaccat tgccatattt tagaacatga aaatctcggt atgatgggga tgtttaaagt
     2161 ggaatagtgg tatctaacta taaaaataga gggatttcgt cataaaaaat ctgcacctta
     2221 atcagttggt ttttagtcca acttttgggg tgcagatcag acgctcactt tgtcgccgat
     2281 ttgaaattcg ccagcgtcat cgcccactgc cacgaccacg cctgcccctg cataacccaa
     2341 agtcgcaggg aacacagggt caatcacata cgccccttcc cgatacatca tctcggcacg
     2401 gtttagtccc aatgcacggc acggttttta aacattttca ttatagtttt ccttatcaaa
     2461 atgggatttg gcaaacattg ggcaaaccta agcccgccca atgtttttag ttaatcaatt
     2521 acttgcaatc acaatcgcca caagtgcagt tttgacaaat gcacagctcg ccattttggc
     2581 agttgcattt tggattgcaa ttttgccctg tgcaagattg gcaaccgcag tgggcgtttg
     2641 ggtttttgat gatgtcgttc ataacaattc tccaaattaa aggttgatta tcaaaacaaa
     2701 aaccgttttt aaacccatta ataaaaaaat gccatttaaa acggtcactt ttgcacaagc
     2761 aacaaccaat gatggtcaat ccttattagc ggttaatgac ttatttattg gccctaaaag
     2821 ccacacttcc gcacagtata ttttgcaatg gaatggcgct gaagaagtgc aatcttcatc
     2881 aggcattatt gtatcaacag gattgggatc aacggggtgg tttcaatcta ttcttgctgg
     2941 tgcgatggca attacaggag aagcttcgca ccctctatta caaggcttta gctggagtga
     3001 tcgaaagcta caatttagtg taagagagcc atttccaagt agaacaacag gtgttgcact
     3061 gacttttggc actattgagc ctgactcacc actgcaatta ggatctttga tgccagagaa
     3121 tggcgtaatt ttctctgatg gcatcgaaga tgactattta caatttaatg caggttgtat
     3181 tgctcacatt ggtatcgctg acatacaagg gcaactaatt agccaaaaag ggcgtcagcg
     3241 aatttagatt tatttagcgc tctttaatac tgtttcaagt ccagagatca tcacatcaag
     3301 gacaaacaaa aatgcagcat caccattatc actatccata attgcaacgg cttgggttaa
     3361 taatggcgga taggcaacag tatccgtctc tactttttca cgctcttttt ggctttcttg
     3421 atgctcttga gtttccagta cggagcctaa tgtaaaatgc gcaatagagc ttaatgcata
     3481 cacggcttga gatagactaa acccagcatc acacaaaaac tgtagttgct gttctgatgt
     3541 ctcaaattga ctttcagagg ggcgtgttcc cgcatgaatt ttgccaccat cacgatacat
     3601 taataaggct tggcggaagc ttttcgcgtt atttcgcaaa aagtcctgcc atgtttcatt
     3661 cggcaatggc aaaacatgat gatggtgctt ttgcaaaata gtttctgcta atgcatctaa
     3721 caaagcgcgt ttatttttta catgccaata caatgtgggt tgttccacac ctattttttg
     3781 cgccagctta cgcgttgtta atccttcaat accaacttca ttaagtaaaa tcaacgcatt
     3841 atcaataact tgttctttat ctagctttgc cattacctac ctcaaaataa aatagccttg
     3901 acaatctatc actgatagag atatattaca cccactctat cactgataga tttttaggat
     3961 ctcaatgaat aaatcaatta ttattatact gctgatcacc gtattagatg ccattggtat
     4021 cgggcttatc atgccagtac tccctactct attaaatgaa tttgtcagtg aaaattcact
     4081 ggcaacccat tacggtgtgc tattagcgct ctatgctacc atgcaggtta tttttgctcc
     4141 tattctagga cgactgtctg ataaatacgg cagaaaaccc atcttgctgt tttccctttt
     4201 aggcgcggca ctcgactatc ttttaatggc attctcaacc acactttgga tgctctatat
     4261 tgggcgcatc attgcgggga tcacaggcgc aacaggtgcc gtatgtgcat cagcgatgag
     4321 tgatgtgact cccgctaaaa atcgaactcg ctattttggt ttcttaggtg gtgcttttgg
     4381 tgttggcctt attatcggcc caatgctagg gggattatta ggtgatatca gtgctcatat
     4441 gccatttatt tttgccgcta tttcacactc gatattatta atactctctt tgctcttttt
     4501 ccgagaaaca caaaaaagag aagcgcttgt tgccaatagg acacctgaaa accaaactgc
     4561 ctcaaataca gtcactgttt tttttaagaa aagcctctac ttttggttag caacctattt
     4621 tattatccag cttatcgggc aaattcctgc caccatctgg gtgctgttta cacaatatcg
     4681 ttttgattgg aacacaactt ctatcggtat gtctttggcg gttctgggtg tattaaatat
     4741 tttctttcag gcgattgtcg ctgggaaatt ggcacaaaaa tggggcgaaa aaaccaccat
     4801 tatgatcagt atgtctattg atatgatggg ctgtttatta ttagcgtgga taggccacgt
     4861 ttgggtcatc ttaccagcat taatttgctt agcggcagga ggtatggggc aacccgcatt
     4921 acaaggttat ttatcaaaat ctgtcgatga taatgcgcaa gggaaattac aaggtactct
     4981 ggtgagccta accaatatta ccgggatcat tggtcccctt ttatttgcct ttatttatag
     5041 ttatagcgtc gcttattggg atggtctgtt atggctgatg ggggcaatac tttatgctat
     5101 gttgcttatt accgcttatt ttcaccaaag aaaaaccaca cctaaagctg ttatttcaac
     5161 cccttaatac tagaaattat aaagatcata aaagtcctgt ccgctcttaa taacgttgat
     5221 aggactttat ttgggtatcc ggattacagg gttgaaacag ggctataaac gccccgcctc
     5281 aatctcccgc accagtgttg atatgtcagc gccgtagtta gtggatggcc ccgcttcggc
     5341 aacgccatag aacggcatcc tgcccatagc gccctcgggt atccgttgac gctgatagcg
     5401 tggcttcttg atcttctgga agccctcagt aaacaggatc ttggctcgca gcgcttcaaa
     5461 gctgtagcca cggccaacac gattaacaac acgaatcagg ttgttcagtg actcggtgta
     5521 ggcgttggta atggggtggt cgaagtaagc gaaaatgtca tcgtgccagt tgcccatcgc
     5581 tttgataagc gggtcatagt gggctttcat ttccggcgta atctgtcgca gccaagagta
     5641 atatgcctcc tgtgcctggt gccgggtctg acagtcccaa atctcgaaaa aggcttcttt
     5701 cagttcgtag gcttggccaa tctcagggta attcaatgtc cagcctgaaa aacgcatgta
     5761 ttcggcatct gtcagctcgt gacggcgttt cagtaggaca aacctatctc gcatcaaccc
     5821 acggcgctgc tgtggcgtca gggcgcttct gatggctttt ctggcgcgtt ccagtgattc
     5881 gttcgccatc ctgacaacgt ggaacttgtc gatgatcacc gtggcgtcag gaatcatggt
     5941 ttctactgct tgccggtacg gcctccacat atccattgcc acgtagcgca ccaggtcgcg
     6001 gtcagtgcgt ttgctcaggt agcgggtgac ggtggtttta ttgcggttat ccagcatgtc
     6061 cacaatggtc tgctgctgaa tgttggtgat cacgcagcgg ggtttgatga tgtggatttc
     6121 gtcgataccc aaccactgcg gcatttcaaa attgagcgtc ttttcgaggc gttcacaata
     6181 gtcgttgaaa atgttacgga cggtcttttc atcgacgccc acatcctcgg caacactgga
     6241 gaacgtgcgg cgcaggctct cgcgctcgat gtactggatc aggcggttgg tcatctgccg
     6301 cttttcgtct ttgtgcggta caggctccag aaacgtcttt cggcaagact ggcagcgata
     6361 gcgacggcgg ttaagcatga tgccggtacg ccttccgtgg acgggtgtat ccatgatcac
     6421 ctcatcacgg cgaccaaagc cgacgatttc aggatgattg cagtgggggc actgcgtagg
     6481 cggttcggaa acttcggcgt gtacctggta atcgtgctcc agttcggata cctggagcac
     6541 tttgtaatca ggccagttga ggatattcga cattactcct ttgccacctt aaccgattcc
     6601 tcggtgccag tggcagcagt gactagcgcg ttatgaactc gacgactggt atcttcactg
     6661 tctgtttcaa tccacgcgcc aaaggcatgc accattgcag caaaagactt cccatcaagg
     6721 atttcaccag acttgcctct gagcaaagcg cccataccat agctgcttga cggatgatcg
     6781 gcagtccacc tgagagtctc gggagacatt tggcgaagcc ccattcgctt tatcgtggcg
     6841 atagactcgt ccggaaaata gagtttgatt ttcatgctga cctcgctgaa agccgcccct
     6901 agggcggcat gttagtcaaa ttgcttcgcc gcggcggatg ttgcgaatac gaaaacggag
     6961 gtgctttgtc gcttggtgaa ccccttggcc gcgtaattct gattctttat tcgggttgtt
     7021 gttgattagg tcttcagcaa ccctttcaag ctcgctaagc gcctcttcga tttcattcag
     7081 cgcatcattt gcatcgtatg acatggcttc tatcctgcac caagttcggc ggtgcgcccg
     7141 ttgggcttat gcccgagccg ttgttctcag ctcttgaatc taatattagg gcatctggcc
     7201 ttttccgtca accctaaaat ccggtttatt
//
LOCUS       DANLYI010000382         4183 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749890-rid16721653.guided.192, whole genome shotgun sequence.
ACCESSION   DANLYI010000382 DANLYI010000000
VERSION     DANLYI010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749890
            Sequence Read Archive: SRR17934078
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574562.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574562.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:13:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,483
            CDSs (with protein)               :: 2,483
            Genes (RNA)                       :: 51
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 16 of 59
            Pseudo Genes (internal stop)      :: 9 of 59
            Pseudo Genes (multiple problems)  :: 1 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4183
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749890-rid16721653.guided.192"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KS"
                     /collection_date="2020-06-30"
                     /collected_by="Kansas State University Veterinary
                     Diagnostic Laboratory, Manhattan, KS"
     gene            47..1073
                     /locus_tag="PXD81_002590"
                     /pseudo
     CDS             47..1073
                     /locus_tag="PXD81_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249101.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="colicin transporter"
     gene            1269..2678
                     /locus_tag="PXD81_002591"
     CDS             1269..2678
                     /locus_tag="PXD81_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF560 domain-containing protein"
                     /protein_id="HDL5538558.1"
                     /translation="MKKYPLLFSLFSPVVWAEKVIEHNTDPISFERQPQAVKFEQNVV
                     KNSQKPTAYQPAYSVNQNVEQQINQAILSQDWATLEPLLSRYPQTENFDPLLWHYAQA
                     ALAYAKKDHQTAVFHYRQLLAKPPSLVYPRFDLALILAEDQQFRAAEQEFKAIQAHLP
                     PELAKIARNTQVQIAENEAWQPDFYLQYTQTDNVNNASSSPIVNVNGRIFRKNADSLP
                     QSAKGVRYGLGLSKLHNVKGNHFVGLTLNYSGIYYWDNRDYREQSVSVSPNYSYRTVA
                     YRLTFSPFIEQNWLGSSRYNVQFGATLNGLRYLNSQWLVSGSFTHLQKRYFEELTASR
                     YNSYQHQLSGGVQWQAVKNGRFFANLDGVREIAREKAQSSNKWLARVGAVFQGERWAT
                     QASIGFGKRYFADKHYLFGYKRLDTEYQANLAVWNRTWHWQGIVPKLNFRYQKIDSNI
                     ADFYSRQNKEVFFTLEKLF"
     gene            2824..3297
                     /gene="gpt"
                     /locus_tag="PXD81_002592"
     CDS             2824..3297
                     /gene="gpt"
                     /locus_tag="PXD81_002592"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HDL5538559.1"
                     /translation="MSEKYTNEKYVVTWDMFHMHARKLAENLLPATQWKGIIAVSRGG
                     LFPAAVLARELGIRHVETVCIASYDHDSQGELNVLHAADTDGEGFIVVDDLVDTGNTA
                     KEIRRMYPKARFVTVFAKPHGAPLVDDYVIDIPQETWIEQPWDLGITFVQPLARR"
     gene            3410..4033
                     /gene="gmk"
                     /locus_tag="PXD81_002593"
     CDS             3410..4033
                     /gene="gmk"
                     /locus_tag="PXD81_002593"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HDL5538560.1"
                     /translation="MSLGNLYIISAPSGAGKSSLINALLADLPRSEVQLSISHTTRQP
                     RPAEQDGVHYYFTSHDEFKQLIEQGYFLEWAEVFGNYYGTSLPMIERSLEQGIDVFLD
                     IDWQGARQIREKVPNVKTVFILPPSKTELEKRLIGRGQDSAETIAKRMAEAVSEMNHY
                     NEFDYVIINDDFQQALAELKAILIAERLSLGSQQIRHQHLITDLLNN"
BASE COUNT         1292 a          783 c          974 g         1134 t
ORIGIN      
        1 gttttataca catctatttt tgtttatctt acaaaaggaa cattttatgt ctatcaaaaa
       61 agtaacttta gccagtttga ttttattggg tttaacggcg tgtagtagtg gtggttcggg
      121 cagtagctca aacaatgtgg caaaaccgaa tactaaaccg caaactactc agcaacaaac
      181 cgataaagcg gcagcagaaa aagcagcaaa agacaaggca gcgaaagaga aagcagaagc
      241 agaacgttta gcagcagaaa aagcagctaa agagaaagcg gaagcagaac gtttagcggc
      301 ggaaaaagca gcgaaagaaa aagccgaagc ggaacgtagc agcgaaagaa aaagccgaag
      361 cagaacgttt agcggcggaa aaagcggcga aagagaaagc ggaagcagaa cgtttagcgg
      421 cagaaaaagc ggcgaaagag aaagcggaag cggagcgttt agcagcagaa aaagaagtag
      481 atcctatagc tattattaaa gcaaaaggta ttgatgatac aaattacggc ttaaaacttg
      541 gtaatagtaa agaaactatt gagggtaaag tcacagtcag aacacagagt tatttatata
      601 atcagccata ttctgtagta acagcacaag cagttcaaag aaatggctgg gaaaacggta
      661 attatattga taaagttgat ggaacagtta cagttaaagg attgaaaaca gagaaattac
      721 caacagaggg tactgcaaca tattcaggaa aagcatttaa ccacgatgga gctaaaggtt
      781 ttaatggtgg tgatctaacc tataatgtta atttctctaa gcgtaccggt tcaggtagag
      841 ttgagggaga atttggaaac ttaatcgtat tagaagaggg gacgattaat aatactcaga
      901 tttcttcaac agtacatggc aataatcctt taaacctttc tggaaaatat acagtcgaat
      961 ttttcggtaa aaatgcagag gaaattgccg gtgaaattca gacaaaagca aatcttactg
     1021 gggaagtgag taattttgga cttgccggca ctcgtggcga tatccaaaaa taaccttttc
     1081 taacgttcaa tgccgtttaa cgaaaaatta aacggcattt gtcttgatat tgcttgtaga
     1141 tacaataaga cacaatagtt tagatttcaa tttacagctt ctaataaccc tctcccacaa
     1201 ggggagagag tgataaatcc cgtagaggcg aacatatagg tccgccccta ccaattttct
     1261 ttatctttat gaaaaaatat ccccttttat tttcgctgtt cagccccgtt gtatgggcag
     1321 aaaaggtgat tgaacacaac actgacccga tttcctttga gcgtcagccg caagcggtta
     1381 aatttgagca aaatgttgta aaaaattcac aaaaaccgac cgcttatcag cctgcgtatt
     1441 cagtcaatca gaatgttgaa cagcaaatta accaagcgat tctcagccaa gattgggcaa
     1501 cgcttgaacc gcttttaagc cgttatccgc aaactgaaaa ttttgacccg ttgttgtggc
     1561 actatgcgca agccgcactt gcctatgcga aaaaagatca tcaaaccgca gtttttcact
     1621 atcgccaact gttagctaag cccccgagtt tggtttaccc tcgttttgat ttggcgttga
     1681 ttttggcaga agatcagcaa tttcgtgcgg cagagcagga atttaaggct attcaagccc
     1741 atttaccgcc ggaacttgct aaaattgccc gcaatactca agtccaaatt gctgaaaatg
     1801 aggcgtggca gcccgatttt tatctgcaat atacccaaac agataacgtg aataatgcct
     1861 catcttcgcc gattgtgaat gtaaatgggc ggattttccg caaaaatgca gacagtttgc
     1921 cacaatcagc aaaaggcgtg cgttatggct tagggttgag taaattgcac aacgtgaaag
     1981 gcaatcattt tgtcggttta acgctcaatt atagcgggat ttactattgg gataaccgag
     2041 attaccgtga gcaatccgta tccgtttcgc cgaattatag ctaccgcacc gtcgcttatc
     2101 ggctgacttt ttcgcctttt attgagcaaa attggctggg gagtagccgt tataatgttc
     2161 aatttggggc aacactcaac ggtttgcgtt accttaattc gcaatggttg gtgtcgggga
     2221 gttttaccca tttgcaaaaa cggtattttg aggagctaac cgcaagccgt tataacagct
     2281 atcagcacca actcagtggc ggtgtgcagt ggcaagcggt caaaaatggg cggttttttg
     2341 caaatttaga cggcgtgcgt gaaattgcac gggaaaaagc acaaagctcg aataagtggc
     2401 tggctcgagt gggggcggtt tttcaaggcg aacgctgggc aacgcaggca agtatcggct
     2461 ttggcaagcg atattttgcc gacaagcact atttgtttgg ctataagcgg ttagacacgg
     2521 aatatcaagc caatttagcg gtttggaacc gcacttggca ttggcagggc attgtgccga
     2581 agctgaattt ccgttatcaa aaaattgaca gcaatattgc tgatttttac tctcgccaaa
     2641 acaaggaagt ctttttcact ttagaaaagc tattttagtg ttggtttttt gtgctaagca
     2701 tacgattgcg tagctgccgg cacaaaaaaa taaatgaaat gatggcgaaa aaagcatttt
     2761 cgggttatcc tagtgcaatt ttttatatcg gggtagcccc caattttata ttaggttgtt
     2821 tctatgagtg aaaaatacac aaatgaaaaa tatgtcgtga cgtgggatat gttccatatg
     2881 cacgctcgca agttggcaga aaatttatta cctgccacac agtggaaagg gattattgcg
     2941 gtgagccgtg gcggcttgtt tccggcagcc gttttagcac gtgagttggg tattcgccac
     3001 gtagaaacag tgtgcattgc cagctacgat cacgatagcc aaggtgagtt aaatgtgtta
     3061 cacgcagccg ataccgatgg cgaaggcttt attgtggtag atgatttagt ggataccggc
     3121 aacacggcaa aagaaattcg cagaatgtac ccgaaagctc gttttgtaac cgtgtttgcg
     3181 aaaccgcacg gtgcaccgtt ggttgatgat tatgtgattg atattccaca agaaacgtgg
     3241 attgagcagc cttgggattt aggcattacc ttcgttcagc ctttggcacg tagataattt
     3301 tgtgtaatgg acaatgaaag ttgtccatta ttttttattt attcctatga aatgtagggg
     3361 cgaatcatca ttcgccctat tcccaaaatc aaataggtat atacaaataa tgagtttagg
     3421 caatctttat attatttctg ccccaagtgg agcaggaaaa tcttccttaa ttaacgcctt
     3481 gttggcggat ttaccccgtt cggaagtaca gctttcgatt tcacacacta cccgtcagcc
     3541 tcgtccggca gaacaggacg gcgtgcatta ttatttcact tcgcacgatg agtttaagca
     3601 gctgattgag caagggtatt ttttagaatg ggcggaggtg tttggcaatt attacggcac
     3661 atcattgccg atgattgaac gtagcctaga gcagggcatt gatgtctttt tggatattga
     3721 ctggcaaggg gctagacaaa ttcgggaaaa agtgccgaat gtgaaaaccg tttttatttt
     3781 gccaccgtca aaaacagaat tggaaaaacg tttaatcggg cgtgggcaag attccgctga
     3841 aaccattgcc aaacgtatgg cagaggcggt gtcggaaatg aaccattaca atgagttcga
     3901 ttatgtgatt attaatgatg atttccaaca ggcattggca gagctaaaag cgattttaat
     3961 tgccgagcgt ttgagcttag gttcgcaaca aattcgtcat cagcatttga ttactgattt
     4021 gttaaataat taaatgaaca gttgttcatt taactggaaa ggcaatctgt ttttgggtag
     4081 aatacgccca ttttcccctc tttattttca aaaggaaatg taatgaaact ttcaaaatta
     4141 gcattttctg tggcgttggc tctaaccgta acggcttgta cca
//
